LOCUS       DAUCWU010000001        95701 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUCWU010000001 DAUCWU010000000
VERSION     DAUCWU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 95701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95701
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.001"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..174
                     /locus_tag="VGY24_000001"
     CDS             <1..174
                     /locus_tag="VGY24_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HEQ4283031.1"
                     /translation="SRSKKKNMLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIA
                     CIYLPYKLKKIID"
     gene            238..1029
                     /locus_tag="VGY24_000002"
     CDS             238..1029
                     /locus_tag="VGY24_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ4283032.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            1029..1664
                     /gene="trmB"
                     /locus_tag="VGY24_000003"
     CDS             1029..1664
                     /gene="trmB"
                     /locus_tag="VGY24_000003"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ4283033.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGMFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            1729..1815
                     /locus_tag="VGY24_000004"
     tRNA            1729..1815
                     /locus_tag="VGY24_000004"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1763..1765,aa:Ser,seq:gga)
     gene            1923..2459
                     /gene="rimP"
                     /locus_tag="VGY24_000005"
     CDS             1923..2459
                     /gene="rimP"
                     /locus_tag="VGY24_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ4283034.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            2634..3791
                     /gene="nusA"
                     /locus_tag="VGY24_000006"
     CDS             2634..3791
                     /gene="nusA"
                     /locus_tag="VGY24_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ4283035.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEEPVVDDVMTAEEV"
     gene            3807..4103
                     /locus_tag="VGY24_000007"
     CDS             3807..4103
                     /locus_tag="VGY24_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ4283036.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            4096..4398
                     /locus_tag="VGY24_000008"
     CDS             4096..4398
                     /locus_tag="VGY24_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ4283037.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            4418..7279
                     /gene="infB"
                     /locus_tag="VGY24_000009"
     CDS             4418..7279
                     /gene="infB"
                     /locus_tag="VGY24_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ4283038.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPSHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVRNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLTSLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            7485..7835
                     /gene="rbfA"
                     /locus_tag="VGY24_000010"
     CDS             7485..7835
                     /gene="rbfA"
                     /locus_tag="VGY24_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ4283039.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDNKS"
     gene            complement(7966..8955)
                     /locus_tag="VGY24_000011"
     CDS             complement(7966..8955)
                     /locus_tag="VGY24_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4283040.1"
                     /translation="MKHPIRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKR
                     DKSYYSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKP
                     DQKTIIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVN
                     LYQHYYHKNQNVFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEI
                     PEYEDADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFY
                     PDIRDYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTPQ"
     gene            9127..9561
                     /locus_tag="VGY24_000012"
     CDS             9127..9561
                     /locus_tag="VGY24_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ4283041.1"
                     /translation="MQQISAAEWEVMRVVWTYGTVKSSDIIMILGKGRKWSDSTIKTL
                     IGRLVKKNLLTSYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYDL
                     PMTLEEIGAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            9561..11792
                     /locus_tag="VGY24_000013"
     CDS             9561..11792
                     /locus_tag="VGY24_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ4283042.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVRDAGYGAQVFDEHAKHMQTGRRDEGLKVIWYQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDWYPFIYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGQAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATDVRVWRSSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESFPVEKGEKDNVFAGSLNGKGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLI
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            11806..12009
                     /locus_tag="VGY24_000014"
     CDS             11806..12009
                     /locus_tag="VGY24_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ4283043.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(12213..12983)
                     /locus_tag="VGY24_000015"
     CDS             complement(12213..12983)
                     /locus_tag="VGY24_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4283044.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            13220..13693
                     /locus_tag="VGY24_000016"
     CDS             13220..13693
                     /locus_tag="VGY24_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4283045.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            13686..13991
                     /locus_tag="VGY24_000017"
     CDS             13686..13991
                     /locus_tag="VGY24_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4283046.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIESQMTDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFDLPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            14017..15468
                     /locus_tag="VGY24_000018"
     CDS             14017..15468
                     /locus_tag="VGY24_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ4283047.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            15725..16150
                     /gene="lacA"
                     /locus_tag="VGY24_000019"
     CDS             15725..16150
                     /gene="lacA"
                     /locus_tag="VGY24_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ4283048.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            16165..16680
                     /gene="lacB"
                     /locus_tag="VGY24_000020"
     CDS             16165..16680
                     /gene="lacB"
                     /locus_tag="VGY24_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ4283049.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLESHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            16690..17029
                     /locus_tag="VGY24_000021"
                     /pseudo
     CDS             16690..17029
                     /locus_tag="VGY24_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            17179..18156
                     /gene="lacD"
                     /locus_tag="VGY24_000022"
     CDS             17179..18156
                     /gene="lacD"
                     /locus_tag="VGY24_000022"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ4283050.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            18353..18970
                     /locus_tag="VGY24_000023"
     CDS             18353..18970
                     /locus_tag="VGY24_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ4283051.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            18972..19796
                     /locus_tag="VGY24_000024"
     CDS             18972..19796
                     /locus_tag="VGY24_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4283052.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(19832..20389)
                     /locus_tag="VGY24_000025"
     CDS             complement(19832..20389)
                     /locus_tag="VGY24_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4283053.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            20682..21524
                     /locus_tag="VGY24_000026"
     CDS             20682..21524
                     /locus_tag="VGY24_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4283054.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(21652..22374)
                     /locus_tag="VGY24_000027"
     CDS             complement(21652..22374)
                     /locus_tag="VGY24_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283055.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSNSGDKQSSKE"
     gene            22576..24207
                     /locus_tag="VGY24_000028"
     CDS             22576..24207
                     /locus_tag="VGY24_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ4283056.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            24325..25473
                     /gene="nagA"
                     /locus_tag="VGY24_000029"
     CDS             24325..25473
                     /gene="nagA"
                     /locus_tag="VGY24_000029"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ4283057.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPEREGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHSEPGMVGAVYNLPNTYAELICDGHHVSPVACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            25595..26434
                     /locus_tag="VGY24_000030"
     CDS             25595..26434
                     /locus_tag="VGY24_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ4283058.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNAWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            26566..27099
                     /locus_tag="VGY24_000031"
     CDS             26566..27099
                     /locus_tag="VGY24_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ4283059.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            27471..28388
                     /gene="glyQ"
                     /locus_tag="VGY24_000032"
     CDS             27471..28388
                     /gene="glyQ"
                     /locus_tag="VGY24_000032"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ4283060.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LSINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            28766..30805
                     /locus_tag="VGY24_000033"
     CDS             28766..30805
                     /locus_tag="VGY24_000033"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ4283061.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGVLPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWCIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAAAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK"
     gene            30959..31216
                     /locus_tag="VGY24_000034"
     CDS             30959..31216
                     /locus_tag="VGY24_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ4283062.1"
                     /translation="MDSKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            31343..31735
                     /locus_tag="VGY24_000035"
     CDS             31343..31735
                     /locus_tag="VGY24_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ4283063.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            32095..33621
                     /gene="glpK"
                     /locus_tag="VGY24_000036"
     CDS             32095..33621
                     /gene="glpK"
                     /locus_tag="VGY24_000036"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ4283064.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            33637..35475
                     /gene="glpO"
                     /locus_tag="VGY24_000037"
     CDS             33637..35475
                     /gene="glpO"
                     /locus_tag="VGY24_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ4283065.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLT
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQT
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGHQEG"
     gene            35477..36178
                     /locus_tag="VGY24_000038"
     CDS             35477..36178
                     /locus_tag="VGY24_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ4283066.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            36287..37633
                     /locus_tag="VGY24_000039"
     CDS             36287..37633
                     /locus_tag="VGY24_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4283067.1"
                     /translation="MTLTIKTIHDIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QSNIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDADLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            37623..39122
                     /locus_tag="VGY24_000040"
     CDS             37623..39122
                     /locus_tag="VGY24_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4283068.1"
                     /translation="MKIEDLMDKERRAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMDQETDFSQQLSDDILALLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRNFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            39248..39892
                     /gene="fsa"
                     /locus_tag="VGY24_000041"
     CDS             39248..39892
                     /gene="fsa"
                     /locus_tag="VGY24_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ4283069.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            40110..42095
                     /gene="tkt"
                     /locus_tag="VGY24_000042"
     CDS             40110..42095
                     /gene="tkt"
                     /locus_tag="VGY24_000042"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ4283070.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     ANEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHDGTRVYGGTFFVFSNYLLPAVRMAALQS
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            42287..42553
                     /locus_tag="VGY24_000043"
     CDS             42287..42553
                     /locus_tag="VGY24_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ4283071.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            42587..43321
                     /locus_tag="VGY24_000044"
     CDS             42587..43321
                     /locus_tag="VGY24_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283072.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            43326..44954
                     /locus_tag="VGY24_000045"
     CDS             43326..44954
                     /locus_tag="VGY24_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283073.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAMFLVIYFFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MMDGVFTDYPTIPYFKGFYDVVQVPFATVALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGDGQWLTMGLIFGNLIVAGVLGFGAVIIANIIQQ
                     PLLISILLSCLILMVLGLAQLWIQKTFWKSLERL"
     gene            45018..45839
                     /gene="proB"
                     /locus_tag="VGY24_000046"
     CDS             45018..45839
                     /gene="proB"
                     /locus_tag="VGY24_000046"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ4283074.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGASSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD"
     gene            45832..47082
                     /locus_tag="VGY24_000047"
     CDS             45832..47082
                     /locus_tag="VGY24_000047"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ4283075.1"
                     /translation="MTDITMLGQRAKQASLAIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKIHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            47102..47263
                     /locus_tag="VGY24_000048"
     CDS             47102..47263
                     /locus_tag="VGY24_000048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283076.1"
                     /translation="MTNEREKEEAKVTLEELLHQIQVIHLELKIVQNWDYFVFLEMVC
                     WSAGSNVEK"
     gene            47365..47529
                     /locus_tag="VGY24_000049"
     CDS             47365..47529
                     /locus_tag="VGY24_000049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283077.1"
                     /translation="MSWDKTFGIWLDNLLTDCCVIKEAKNLLQAVETLEAVAKSVLAK
                     LADSEQKEQD"
     gene            47553..48566
                     /gene="rsmH"
                     /locus_tag="VGY24_000050"
     CDS             47553..48566
                     /gene="rsmH"
                     /locus_tag="VGY24_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ4283078.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLAFDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            48571..48894
                     /gene="ftsL"
                     /locus_tag="VGY24_000051"
     CDS             48571..48894
                     /gene="ftsL"
                     /locus_tag="VGY24_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ4283079.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            48898..51153
                     /locus_tag="VGY24_000052"
     CDS             48898..51153
                     /locus_tag="VGY24_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4283080.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            51155..52165
                     /locus_tag="VGY24_000053"
     CDS             51155..52165
                     /locus_tag="VGY24_000053"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ4283081.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLAGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQGQFDVLLLIGTMIGALLGFFL
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVILQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            52323..53666
                     /locus_tag="VGY24_000054"
     CDS             52323..53666
                     /locus_tag="VGY24_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4283082.1"
                     /translation="MSFKDYHFKQYIQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDETKAEVQVVITAPSRELATQIFDACKQIAKHSQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            53861..54664
                     /locus_tag="VGY24_000055"
     CDS             53861..54664
                     /locus_tag="VGY24_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4283083.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKCLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            54664..55407
                     /locus_tag="VGY24_000056"
     CDS             54664..55407
                     /locus_tag="VGY24_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283084.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            55511..55735
                     /locus_tag="VGY24_000057"
     CDS             55511..55735
                     /locus_tag="VGY24_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ4283085.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            55799..56716
                     /gene="trxB"
                     /locus_tag="VGY24_000058"
     CDS             55799..56716
                     /gene="trxB"
                     /locus_tag="VGY24_000058"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ4283086.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIKGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKNLEITDSEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            56885..58264
                     /locus_tag="VGY24_000059"
     CDS             56885..58264
                     /locus_tag="VGY24_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ4283087.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            58435..59889
                     /locus_tag="VGY24_000060"
     CDS             58435..59889
                     /locus_tag="VGY24_000060"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ4283088.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVKEFDAIPLLVDIFVKGELVYQLPTLAEIKAYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            59891..60715
                     /gene="nadE"
                     /locus_tag="VGY24_000061"
     CDS             59891..60715
                     /gene="nadE"
                     /locus_tag="VGY24_000061"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ4283089.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            60900..62237
                     /gene="pepC"
                     /locus_tag="VGY24_000062"
     CDS             60900..62237
                     /gene="pepC"
                     /locus_tag="VGY24_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ4283090.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQY"
     gene            complement(62704..64869)
                     /locus_tag="VGY24_000063"
     CDS             complement(62704..64869)
                     /locus_tag="VGY24_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4283091.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDTSNSTNTDNNGSHPSTDDKKTTH"
     gene            complement(64856..65464)
                     /gene="recU"
                     /locus_tag="VGY24_000064"
     CDS             complement(64856..65464)
                     /gene="recU"
                     /locus_tag="VGY24_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ4283092.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            65545..66060
                     /locus_tag="VGY24_000065"
     CDS             65545..66060
                     /locus_tag="VGY24_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ4283093.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLISYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPMQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            66182..66508
                     /gene="gpsB"
                     /locus_tag="VGY24_000066"
     CDS             66182..66508
                     /gene="gpsB"
                     /locus_tag="VGY24_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ4283094.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            66522..66890
                     /gene="rnpB"
                     /locus_tag="VGY24_000067"
     ncRNA           66522..66890
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGY24_000067"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            66953..68107
                     /locus_tag="VGY24_000068"
     CDS             66953..68107
                     /locus_tag="VGY24_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ4283095.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWENYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKINGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            68121..69590
                     /locus_tag="VGY24_000069"
     CDS             68121..69590
                     /locus_tag="VGY24_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283096.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALESSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDAVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(69736..70218)
                     /locus_tag="VGY24_000070"
     CDS             complement(69736..70218)
                     /locus_tag="VGY24_000070"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ4283097.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(70292..71623)
                     /locus_tag="VGY24_000071"
     CDS             complement(70292..71623)
                     /locus_tag="VGY24_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEQ4283098.1"
                     /translation="MEIIGSLGVLIGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTSTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(71694..72473)
                     /locus_tag="VGY24_000072"
     CDS             complement(71694..72473)
                     /locus_tag="VGY24_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ4283099.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETFLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(72506..73165)
                     /locus_tag="VGY24_000073"
     CDS             complement(72506..73165)
                     /locus_tag="VGY24_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ4283100.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(73167..73817)
                     /locus_tag="VGY24_000074"
     CDS             complement(73167..73817)
                     /locus_tag="VGY24_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ4283101.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(73841..75028)
                     /locus_tag="VGY24_000075"
     CDS             complement(73841..75028)
                     /locus_tag="VGY24_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ4283102.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            75229..76125
                     /locus_tag="VGY24_000076"
     CDS             75229..76125
                     /locus_tag="VGY24_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4283103.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSISIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCIPMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            76255..77862
                     /locus_tag="VGY24_000077"
     CDS             76255..77862
                     /locus_tag="VGY24_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ4283104.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            77972..78607
                     /gene="gmk"
                     /locus_tag="VGY24_000078"
     CDS             77972..78607
                     /gene="gmk"
                     /locus_tag="VGY24_000078"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ4283105.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            78623..78940
                     /locus_tag="VGY24_000079"
     CDS             78623..78940
                     /locus_tag="VGY24_000079"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ4283106.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            79005..81389
                     /locus_tag="VGY24_000080"
     CDS             79005..81389
                     /locus_tag="VGY24_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ4283107.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERPIDRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            81451..82386
                     /locus_tag="VGY24_000081"
     CDS             81451..82386
                     /locus_tag="VGY24_000081"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ4283108.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMELGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIAAGQGA
                     LSLLVVQPAGKPKMAIADFLNGLGRKLEVGDFIGR"
     gene            82376..83698
                     /gene="rsmB"
                     /locus_tag="VGY24_000082"
     CDS             82376..83698
                     /gene="rsmB"
                     /locus_tag="VGY24_000082"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ4283109.1"
                     /translation="MADNWKISIRGKALLTLEHIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEAAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            83736..84476
                     /locus_tag="VGY24_000083"
     CDS             83736..84476
                     /locus_tag="VGY24_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ4283110.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            84473..86371
                     /gene="pknB"
                     /locus_tag="VGY24_000084"
     CDS             84473..86371
                     /gene="pknB"
                     /locus_tag="VGY24_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ4283111.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            86494..87186
                     /locus_tag="VGY24_000085"
     CDS             86494..87186
                     /locus_tag="VGY24_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ4283112.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            87183..88187
                     /locus_tag="VGY24_000086"
     CDS             87183..88187
                     /locus_tag="VGY24_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283113.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            88180..88821
                     /locus_tag="VGY24_000087"
     CDS             88180..88821
                     /locus_tag="VGY24_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4283114.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            88858..90258
                     /locus_tag="VGY24_000088"
     CDS             88858..90258
                     /locus_tag="VGY24_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4283115.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVEEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAAIGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEEVIIETIEVFD"
     gene            90258..90635
                     /locus_tag="VGY24_000089"
     CDS             90258..90635
                     /locus_tag="VGY24_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ4283116.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(90653..91594)
                     /gene="cysK"
                     /locus_tag="VGY24_000090"
     CDS             complement(90653..91594)
                     /gene="cysK"
                     /locus_tag="VGY24_000090"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ4283117.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLIGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(91722..92354)
                     /locus_tag="VGY24_000091"
     CDS             complement(91722..92354)
                     /locus_tag="VGY24_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ4283118.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            92410..93735
                     /locus_tag="VGY24_000092"
     CDS             92410..93735
                     /locus_tag="VGY24_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4283119.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            93707..94372
                     /locus_tag="VGY24_000093"
     CDS             93707..94372
                     /locus_tag="VGY24_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ4283120.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            94452..95000
                     /gene="raiA"
                     /locus_tag="VGY24_000094"
     CDS             94452..95000
                     /gene="raiA"
                     /locus_tag="VGY24_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ4283121.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT        29698 a        17020 c        20395 g        28588 t
ORIGIN      
        1 agtcggagca agaagaaaaa tatgttaagt tttttacgag gtttaagctt tttaatgtta
       61 atcgtcaata tgctgtgttg ctcatcagcg ccaaaagcac tcattttgat tgtaggtatg
      121 gttttcatag cttgtatata tctaccttat aagttaaaga agatcattga ctaagtagcc
      181 taaaacaagt aaaatagggg taaacgacta taaaagatag aaaaggaggg cgataaggtg
      241 acaacgacgg aacaagaact taccttgact cccttacgtg ggaaaagtgg caaagcttat
      301 aaaggcactt atccaaatgg ggaatgtgtc tttataaaat taaatacgac ccctattcta
      361 cctgccttag caaaagaaca gattgcgcca cagttacttt gggccaaacg catgggcaat
      421 ggtgatatga tgagtgccca agaatggctt aacggccgta cattgaccaa agaagatatg
      481 aacagtaagc aaatcattca tattctattg cgccttcaca aatctaaaaa attagtcaat
      541 caactgcttc agctcaatta taagattgaa aacccatacg atttattggt tgattttgag
      601 caaaatgcac ccttgcaaat tcagcaaaat tcatatttac aagctatcgt taaagaatta
      661 aaacggagct taccagagtt caaatcagaa gtagcaacga ttgtgcatgg agatattaaa
      721 catagcaatt gggtgattac tactagtggt atgatttttt tagtagattg ggattctgtt
      781 cgtctgactg atcggatgta tgatgttgct tacctgttga gccactatat tccacggtct
      841 cgttggtcag aatggctgtc ttattatggc tataaaaata atgacaaggt tatgcaaaaa
      901 attatttggt atggtcaatt ttctcacctg acacaaattc tcaagtgttt tgacaagcgt
      961 gacatggagc atgtgaatca ggagatttat gccctcagaa aatttagaga aatatttaga
     1021 aagaaataat gcgagttaga aaacgaaaag gtgctgagga gcacttagct aataacccac
     1081 actatgtgat attaaatcca gaagatgcca agggccgttg gcatgacgtt tttggaaatg
     1141 accgtcctat tcatatcgaa gttggttcag gaaagggcgg atttatcaca gggatggcct
     1201 tgaaaaatcc tgatattaac tacattggca ttgatattca attatctgtc ctaagctacg
     1261 ccttggataa ggtattagct tcagaagtac ccaatgtcaa acttctgcga gtggatggat
     1321 caagtttaac caactatttt gaagatggtg aggtggatat gatgtacctc aacttttcag
     1381 atccttggcc aaaaaccaag catgaaaaac gtcgtttgac ctacaaggat ttcttagaca
     1441 cttacaagcg tatcttgcca gaacacggtg aaattcattt taaaaccgat aatcggggca
     1501 tgtttgagta cagtttagct agtttttctc aatacggcat gaccttgaga cagatctggc
     1561 ttgacttaca cgctagtaac tacgaaggaa atgtcatgac agaatatgaa gagaagttct
     1621 ctaataaagg tcaagtcatt taccgagttg aggcaaattt ctagtagtat tttgtttcaa
     1681 aatatgataa cataaaggag ttgatacagt attaacttct ttagtaatgg agaggtcgca
     1741 tagtggctta gtgcgcacgc ttggaaagcg tgtagtcctt aacggggctc gggggttcga
     1801 atcccctcct ctccttgtga gtaaaacaaa gtgcatatcc ttgcattcct cttaaattat
     1861 ggtataatag gcttaaataa tacagaaagg tggtgggata tgtctcgcca cttttgtatt
     1921 aagtggattc ccaaggacca attatcttag agaagagcat aaaaatagag gaggtaatca
     1981 aaatagcaaa tacatctatt atcgatatcg taaccaagac agtaacacct gagattaagg
     2041 caccttatga attagtagac gttgagtatg acaaaatggg tagtgactac atccttagta
     2101 ttttagtcga taaggaaggt ggaatcacag tggaagatac atctgacttg acgaatatta
     2161 ttagtccgct gttagatacg attgatccag atccttttcc aaaccaatat atgctagaag
     2221 tctctagtcc aggtttggag cgtccgctta agacagctga cagtttaaaa gcagcagttg
     2281 gctcttatat caatgtgagt ctttatcaag ctattgataa ggtgaaagtt tttcaaggag
     2341 acttattagc ctttgatggt gaaacgttga ccattgatta tcttgataaa acacgtcata
     2401 agatcgtgaa tataccatat caagcagttg ctaaggtgag aatggctgtc aaattataac
     2461 ttttatttca aagcactaaa cttttttagg aacgcttaag caaatttgaa acaacaccta
     2521 tagagtttat acaagctaaa caaaaggaat taagggagcc tttagggggc tattgatcgt
     2581 attaatttaa gaaaggattt ttgccaagct ttagaagcaa ggtagataac attatgagca
     2641 aagaaatgct agaagccttc cgtattttgg aagaagaaaa acacattgat aaagcagata
     2701 ttattgacgc agtgacagaa tcattaaagt ctgcctataa acgtcgctat ggtcaatcag
     2761 aatcatgtgt tattgaattt aatgaaaaaa cagctgattt tcaagtattc acggttcgtg
     2821 aggttgttga agaagttttt gacagccggc ttgagattag tttgaaagat gctcttgcta
     2881 tcagttctgc ctatgaattg ggtgataaga ttcgctttga agaatctgtc aatgaatttg
     2941 gacgtgtcgc agctcaatca gctaaacaga ctattatgga aaagatgcgc cgccaaatgc
     3001 gtgaagtgat gttcaatgaa tacaaagagc atgaaggtga aattatgaca ggaacagttg
     3061 aacggtttga tcaacgtttt atttatgtca atcttggttc cttagaagca caattgtctc
     3121 accaagacca gattccaggt gaaactttca aatcacatga ccgtattgac gtttatgtct
     3181 acaaagttga aaataatcct aaaggagtta acgtctttgt tagtcgtagc catccagaat
     3241 tcatcaaacg tattatggag caagaaattc ctgaagtctt tgatggtact gttgaaatca
     3301 tgagtgtttc tcgtgaagca ggtgaccgca ccaaggttgc tgtgcgcagt cataatccta
     3361 acgttgatgc catcggaaca attgttggac gtggtggaag taatattaaa aaggtcatca
     3421 gcaagttcca tccaaaacgt gtagatgcta agacggggct tgaaattcca gttgaagaaa
     3481 atattgacgt gattcaatgg gtagatgatc cagctgagtt catctacaat gccattgctc
     3541 cggctgaagt agacatggta ctttttgacg atgaagatct taaacgtgct acggtagtag
     3601 ttcctgatag caaattatca ttagctatcg ggcgtcgcgg ccaaaatgtt cgtttagcgg
     3661 cacatttgac tggctaccgt attgatatca aatcagctag cgaatatgat cgtcttgagg
     3721 cggaaaaaga agcagcaaca gctgtggaag agccagttgt tgacgatgtt atgactgccg
     3781 aggaagtata agaaaagagg tgtttgatgt ctaaagtcaa aaaaatacct ttacgaaaat
     3841 cacttgtttc tggtgaaatt attgctaaac gagatcttct taggattgta aaaactaagg
     3901 atggtcaagt tttcattgat ccaactggta aacaaaatgg tcgtggtgct tatatcaaat
     3961 tagacaatca agaggcctta atggccaaga aaaaacaagt tttcaacagg agtttctcaa
     4021 tggacattcc agaaagtttt tacgatgact tgattgctta tgttgatcac aaaatcaaaa
     4081 gaagagagtt aggtcttgac taatttagaa agattatcta gtcttattgg tctagctcaa
     4141 cgggctggaa aagtgatttc aggtgaagaa ctagtagtaa aagctattca acaccagcag
     4201 gtaatattag tatttttagc caatgatgct ggtcctaatg tgacaaaaaa agtgacagat
     4261 aaaagtaatt attataatgt agaagtctcc acagtgttga atgcactgga attaagtgct
     4321 gcactaggta aaccaaggaa ggttgctgcc attgcagatg ctggattttc aaagaaaatg
     4381 aggactctta tggaatagat gaataggagg acacaaattg tcaaagaaaa gattacatga
     4441 aattgccaag gaaattggta aaagtagtaa agaggtggtt gaacacgcta aatacttagg
     4501 attagatgtg aaaagccatg catcaagtgt tgaagaagcg gatgcgaaaa agattatttc
     4561 aagtttttca aaggcttcta aacctgatgt gacagcttct caaacagtca aacccaaaga
     4621 agttgctcaa ccctctgtaa cagtggtcaa agaaacaggt tctgagcatg tagagaaaac
     4681 acaggtctct aagccaaaga gtcgtaattt taaagctgaa agagaggctc gtgcaaagga
     4741 acaggctgct cgaaaacaag caaatggctc ttctcatcgt tctcaagaac gtcgaggagg
     4801 ttatcgccag cccaataatc atcaaactaa cgaacaagga gataagagga ttacccatcg
     4861 ttctcaaggt gatacaaacg ataagcgtat tgagcgaaaa gcatcgaatg tatcacctag
     4921 ccatgataat catcagctag ttggtgatcg taaccgttcc tttgctaagg agaatcacaa
     4981 gaatggtcga ttcacaaacc aaaaaaagca aggacgacaa gagcctcaat cgaaaagtcc
     5041 taaaattgat ttcaaagctc gtgctgctgc cttaaaagct gaacaaaatg ctgaatactc
     5101 acgccaaagt gaaacacgtt ttcgtgcaca gcaagaagct aagcgtttag ctgagttggc
     5161 aagacaagaa gctaaagaag cggctctaaa agcacaagct gaggagatga gccatcgtga
     5221 agcagcattg aaatctattg aagaagcaga aactaaatta aagtcatcaa atattagtgc
     5281 taaatctact gctgataatc gccgtaaaaa acaagcacgt cctgaaaaga atcgtgagtt
     5341 aactcatcat tcacaagaag gacaaaagaa aaataagaag agttggaata gtcaaaacca
     5401 agtgagaaat caaaaaaata gtaactggaa taaaaataaa aagaccaaaa aaggcaaaaa
     5461 tgttaggaat accaacaccg ctccaaaacc agtaaccgag cgtaaattcc atgaattgcc
     5521 aaaagaattt gaatatactg aaggcatgac agttgccgaa atcgcaaaac gtatcaaacg
     5581 cgaacccgct gaaattgtta aaaagctgtt tatgatgggt gttatggcaa ctcaaaacca
     5641 gtctttagat ggggatacta tcgaattatt gatggtagat tatggtattg aggctaaggc
     5701 taaggtagaa gtagatgatg ctgatatcga gcgcttcttt gaggatgaaa attacctcaa
     5761 tcctgaaaat attgtagaac gcgcacctgt tgtaactatc atgggacacg tagaccacgg
     5821 taagacaact ttgcttgata ctttgcgtaa ctcacgtgtg gcaacaggag aagctggagg
     5881 aattactcag catattggtg cttaccagat tgaagaagct ggcaagaaaa ttaccttctt
     5941 ggatactccc ggacacgcgg cctttacaag catgcgcgca cgtggagctt ccgttaccga
     6001 tatcacgatt ttgattgttg ctgctgatga tggtgttatg ccacaaacga ttgaagcgat
     6061 caatcactca aaagctgctg gtgttccaat tattgttgct atcaacaaaa ttgataaacc
     6121 aggtgctaat ccagaacgtg ttatcgcaga attggcagaa tatggtatca tctcaactgc
     6181 ttggggcgga gagtgcgaat ttgtagagat atctgctaaa tttaataaaa atattgatga
     6241 actattagaa acagtccttt tggttgctga agtggaagaa ttgaaagctg atccgacggt
     6301 tcgtgctatt ggtactgtga ttgaagcacg cttagataaa ggtaaaggtg ctattgctac
     6361 gcttcttgtc caacaaggaa ctcttcatgt tcaagaccca atcgttgttg gtaatacctt
     6421 tggtcgtgtc cgtgctatgg taaatgacct tgggcgtcgt gttaaatctg cggagccatc
     6481 aacaccagtt tccattactg gtttgaacga aacaccaatg gctggtgatc actttgctgt
     6541 ttacgctgat gaaaaagctg cgcgtgcagc tggtgaggag cgatcaaaac gtgccctcct
     6601 caaacaacgt caaaatacac agcgtgttag ccttgataat ctctttgata cccttaaagc
     6661 aggagaaatc aagactgtta atgtcatcat caaagcagac gttcaaggtt ctgttgaagc
     6721 ccttgcagca tctcttgtaa agattgaagt ggaaggtgtc cgtgtcaatg tcgttcactc
     6781 tgctgtaggt gctatcaatg agtctgatgt gacccttgct gaagcatcaa atgctgttat
     6841 tatcggtttc aacgttcgcc ctaccccaca agctcgtcaa caagcagata cggatgatgt
     6901 ggaaattcgt cttcatagca ttatctataa agtcattgaa gaagtagaag aagccatgaa
     6961 agggaaactt gaccctgttt accaagaaaa aattcttggt gaagccatta ttcgtgaaac
     7021 cttcaaagtt tctaaagtgg gaacaattgg tggctttatg gtaatcaacg gtaaggtgac
     7081 acgtgattcg agtgttcgtg ttatccgtga tagtgtcgtt atttttgatg gtaaacttac
     7141 cagcttgaaa cattacaaag acgatgtcaa agaagtcggt aatgctcaag aaggtgggtt
     7201 gatgattgaa aactttaacg atcttaaagt tgacgatact attgaagcct atattatgga
     7261 agaaattgtg agaaaatagg ggttaagaag gtctgagatg taacgttttt cctctgtcgg
     7321 aaataatgac ttgttctacc agatgagcta cttggggcac tccttccagt ctcgtcatgg
     7381 aattctcaga ttccctttaa ttgtcgttat caaaacaggt agcaatgtca taagctgggc
     7441 tttcagccta gcttttgttt taaaaatgtg gaaaggaaat ggctatggct aatcatcgta
     7501 ttgaccgcgt tgggatggaa atcaagcgcg aagtaaatga tattcttcag aaaaaagtgc
     7561 gtgatccacg cgttcaggga gtaaccatta cagaagtgca aatgcagggt gacttgtcac
     7621 ttgctaaagt ttattacact attatgagtg acttggcatc tgataatcaa aaagcacaaa
     7681 cagggcttga aaaagcgact ggaacgatta aacgtgaact tggcaaacaa ttgaccatgt
     7741 acaaaattcc agatttggtg tttgaaaaag acaattccat cgcctatggt aataaaattg
     7801 atcaactctt acgagaactt gacaacaagt cctaagtatt gcggtgcgga tgttatgaca
     7861 gaaagagcct tatcattatg atagggctct ttttaattgt tattttgtgg tgcaaactac
     7921 aaaataacca ccagattaac tggtggcagt taggaattat tcccctcatt gaggagtctt
     7981 gttgatggca tcaatgattt ggtattgagc tgtttttgcc tcttcgatag gataaattgg
     8041 gtaaacgtgg ttcatacctt cttgggtaat aaaggtaata ttgtggttag cggctttcag
     8101 ttttgcggca taatcgcgaa tatcaggata aaagatctct cgtgtcccag taaataaagt
     8161 aattggtggt agataagtga tcggaccatt tttaggactg acataaatat ggttggtatt
     8221 atcagcagag taagcccaca attctcccac tcgttttaat ccccaagaag atagaatagg
     8281 atcagcatct tcgtattctg gaatctctgg gtgtgacata gtaacatcta accaaggtga
     8341 taaaagaacg agctgctttg gttgagggac tgattcattg tgtagggcat gagctagacc
     8401 taaggctaaa cctccacctg ctgaatctcc cataaggaaa acattctgat ttttatggta
     8461 atagtgttgg taaagattga ccaattttgg catagtatag ttataggtat aacgtggggc
     8521 tttaggataa atgggtaaaa caattttagc gtcaagactt gtggataatg tctttaacat
     8581 attaatatgg tatgtcgttg gattgttaag ataagaacca ccggcaagat aaataattgt
     8641 tttttgatca ggttttccat tatcattcca agagtaaacc tgcatgccat aaagattttc
     8701 ttgaacaagg ggaccattga ctaaattagt atctagttga taaggtttat cagattgttc
     8761 acgacaggca tcaaggtatc tttggaagct tgggtcatct tgtttagaat agtaactctt
     8821 gtcacgtttt aaccagaaat attgctcgat aaaccagctc ttccaagaac gagaactagc
     8881 tgcaactgga gtcggcaggc ataaggtcag taatagacct aaggtgacaa gtgtttttcg
     8941 aataggatgt ttcaagggta tctccttaat gtaaatgtta tttttattgc aataatatta
     9001 tagtaaaaac gattcatttg tcaatataag gatagaaaaa tgagaaaagt tatttattta
     9061 caaatgtagt taaattcgtt ataattaatt tacaaacgta aatatattgt gaatgaaggt
     9121 gacgttatgc aacagatatc agctgccgag tgggaagtca tgcgcgtggt ctggacctat
     9181 ggtactgtta aaagtagtga tattattatg attctaggaa aaggtaggaa atggtcagat
     9241 tccactatta agaccctcat tggtaggcta gttaaaaaaa atcttttaac gtcttatcgg
     9301 caaggaagag cttatattta tcaagctctt ttggatgaga ctttgctaca aaaagaagca
     9361 ctagcgactg ttttagacgg tatttgtcag cggcaacaca cgcgtctact tcttgaacgg
     9421 ctttatgact taccgatgac cttagaggaa atcggggcat tccaagagct actagaagtc
     9481 aaaaaagaaa atgcagttct tgaggtgccg tgtaattgcc tgcctgggca gtgccactgt
     9541 cagaaaaagg agacgtatta atggctaaag agatattttt agtggaggga atgagttgtg
     9601 cctcttgtgc tctaacaatt gaaaaaacgg tcaatcaatt gcctgaagta gaacaggccg
     9661 ttgtgaattt agcgaccgag aagctcacgg ttacttatca agatgacgat ttagaaacgg
     9721 ccaaagttat tcaagcggta agagatgctg gttatggtgc acaagtcttt gatgaacatg
     9781 caaaacacat gcaaacaggt cgtcgtgacg aaggcctaaa ggttatttgg tatcaggtct
     9841 tgtggtctgc cctattcacc attcctatat tatacctagc tatgggtcac atggtgggct
     9901 tatggcttcc tccatttctg caaccagatt ggtatccttt catttacagc tttagtcagc
     9961 tactcttgac gattccagtc ttagtattaa atcgtcatta ttaccataat gggtttaaag
    10021 ctttgtttaa gggacatcct aatatggact ccttggtagc tttagcaacc agctttgctt
    10081 ttgcttatag tctttatgga gtggttgaaa tcagtttggg tcaggcgcac tttgtgcact
    10141 ccctttattt tgaatcggtg gtagtgattt tgacactcat cggtctagga aagtattttg
    10201 aaagtcgttc taagggccgt acttctcaag ctattcaaaa gctcctatcc ttgaaggcta
    10261 ctgatgtgag ggtatggaga agcagccaat ggcaattgtt accattagaa gaagttagct
    10321 atgatgacct tgtcctggtt caaccaggtg aaaaagtttc tattgacggt attatcgtgg
    10381 aaggacatag cagtttggat gaatcctttt taacagggga aagttttcct gttgaaaagg
    10441 gagaaaaaga taatgtcttt gcgggcagct tgaatgggaa aggcgccctc acgattaaac
    10501 cagataagct tgggaatgat accttgttag cacaaattat ccaactagtt gagaatgctc
    10561 aagaaaataa agcacctatt gcagctattg ctgatagagt ctcaggtgtc tttgttccgg
    10621 tggttttggt attagctctg ttaacagggg tgttttggtt ggtattcatg caggaaagtc
    10681 ttcgttttag tttgacaaca gccatcgctg ttctagtcat tgcttgtcct tgtgcgcttg
    10741 gtttagcaac cccgacagcg attatggttg gaacagggcg tgcagctgaa aatggtattc
    10801 tttttaaagg tggtgacatc ttagaacaag cccatcaggt agatacggtt gtttttgata
    10861 aaacggggac gattacggag gggaaaccaa gtcttcaaaa gctcctaatt ttttcgggag
    10921 atgatcacct tatcttgcaa gaagctgctt ccttggaggc ctattctcaa caccccttag
    10981 gggaggctat tgttcgtgct gctcaggaag ctggttacga tagtctacca gtggaagagt
    11041 ttgagtcttt aaccggccta ggagtcactg gtcagcttga agggcgtcgt ttggcgattg
    11101 gaaatgctgc tttaatggga attcttggta tcgatttatc ttgtgttcag tcagtttgtg
    11161 ctaaggcttc aggaaaaggc caaacccttg tatattatgc taaagagggg cagctacgag
    11221 ctcttttcag tattgctgac gctgtcaaag aagatagtca agctaccgtc gaagctttgc
    11281 atcaactggg aattcacacc atcatgctaa caggtgacca cgatgctacc gctaaggcta
    11341 ttgcctctca agtggggatt acagatgtga tcagtcaagt cttacctgat caaaaagcag
    11401 gcgtgatagc agacctccgc tctcaaggga gaaaagttgc aatggtaggt gatggtatta
    11461 atgatgctcc ggcattggct gtagcagaca ttgggatagc tatggggtca gggacagata
    11521 ttgccattga atcggcggat gtgattctga tgaaacctga tatgctggat ttggtgaaag
    11581 ctatgtcatt gagtcgagcg actatgagga ttgtcaagga aaatttattt tgggcattca
    11641 tttacaatgt cctcatgatt ccagtggcca tgggcttact tcatcttttt ggaggtccac
    11701 tattgaaccc gatgttagca ggctttgcca tgagttttag ttcagtatca gttgttttga
    11761 atgccctgcg attaaaagga aaaaccattt gaaggagaaa acgaaatgga aaaacattat
    11821 caggttacag gaatgacgtg tgacggttgt gcaagaacgg ttactgaaaa attatcagct
    11881 gtccccggtg tgcaatcggt ccaggtaaat ttggaaaaag gacaggctaa ggtgacagga
    11941 cgtcctttaa cgttcctact caagcgtgct ttgaaggata ccaaatttga attacataaa
    12001 tggtcttagg ttattttggt agccttactt catctcaaaa gaggttccag ttttttaatc
    12061 tgggcttctt tttttgtggt cactcataaa gactctgacg ttagcaggtg tgttccgagc
    12121 aaaacaaaaa cacctctctg ggggcaaatc acttttgcca gaaggtgttt aaggacgatg
    12181 cgctgacagg ctgtccattc ttgatgaaac ggttactcct tgttttcgtg ggctactaat
    12241 aattgcgtga actgactata acgttcgatg acgtcctcag ttatttgcga atctgtaaca
    12301 acagcatcaa tgttgtttaa atggtaaaaa atgaagaagt cgtaggcatt gaatttttgg
    12361 ttatctacca agaggtattt ttcaatagaa ttattaaaag ctagcttttg aatctctcct
    12421 tcagtttcgc tataagttgc aatatcgttt ttataaatac cattacagct aataaaagcc
    12481 ttggcaaatt ttaaggatga gatattttga ctggcaaggg accctacaaa agctcctgta
    12541 atagatcggt attcaccacc gataagaatc aggtcaatgg tttcgctgtc ctgaaggatg
    12601 ttaaagacag ggaggctatt ggtcacaatg cgaatctgac gattttttaa ttgctcagcg
    12661 aaacattcta aagttgttcc tggaccaata aagattgttt caccatcatt gactagttgt
    12721 gtcgcatatg aagctagttc ccatttttcc tttgtttgaa cggtttgttt ttcaatattt
    12781 gaccgctctt ttttattagg catggtaata ctttgtgcac ctccatggat gcggatcaat
    12841 ttgccggctt tttccagttc atctaagtct ctacgaactg tcatatcaga aacgtttaaa
    12901 gtctgaataa tgtcattcac agtaacaata ccttgctcat tgactatttc ggtaattttt
    12961 agaagtcttt ctctttttaa catctaaggg gctcctttct atagtttatt tacttatgtt
    13021 tatcatagca tatagacctt catatttcaa taaaacaaaa taaacaaaac aaaaacaaac
    13081 atatagaaca aaaaatattg cgtaaaaatg ttgaattatg ttgaaggtta tactataatg
    13141 attttgtaaa cggttaccaa tttgttgatg atgagatgac agttgttaaa aattgatact
    13201 tatagaaagg cggtagggag tgtttcctaa tattctattt actgaagcaa ggacgcagcc
    13261 agagcttttt gatcttgttg ctagccactt ggaaaaggtt gggtatgtga cgcaagagta
    13321 ccatcaggct ttagtggagc gtgaggcggt atttccgaca ggactcaaag tggacctcaa
    13381 agatggatca gatattttgt atgcggctat tccgcataca gaaaccaagt attgcttggt
    13441 tgatcaagtg gtctatgtta gaaatagtca agcccttacc tttaagcaca tgattaatcc
    13501 agaggaagac tgtttggtaa cggatttctt ctttattatc aatagtcaaa acgagggtca
    13561 aacgacgatt ctatccaatc tcattacatt ctttattacg aaaggcaatc tgagctattt
    13621 agccagtcta aaagatgata aacaagctat ttcaaactat ttaatagaaa aaggagtgtt
    13681 tcagcatgat taaaattcta gcagcctgcg gtgcaggggt caactcaagc catcaaatta
    13741 aagatgctat cgaaagccaa atgactgatc gtggctatga tgtgcattgt gatgcggtga
    13801 tggtcaaaga catcacagaa gaattggtgt ctcgttatga tatttttacg ccgattgcaa
    13861 aaactgactt gggatttgac ctgccgattc ccatagtaga agcaggaccg attctctatc
    13921 gtatcccaat catgagtgag cctgtttttg ctgaattaga gcgcgtgatt aaagaaaatc
    13981 atttgcagtg agttaataaa aacataagga gacaagatga tgcaaccttt tttagacatt
    14041 attaacaaga ttctgggatt tcctattcag ttgggatcag gtgtggtcat gctgattgtc
    14101 atgacaggtt tagccatgat tttcggggtt aaattcacaa aggcactgga aggtgggatt
    14161 aagttagcca tcgctttgac agggattgga gccattattg gaattttgac gggggctttt
    14221 tcagagtctt tacaggcctt tgttaaaaat acagggatca gtttaaacat tattgatgtc
    14281 ggttgggcac cgcttgcaac aattacctgg ggttcccctt acacccttta tttcttgctg
    14341 gtgatgctgg tggttaatat tgtcatgatt gtcatgaaaa aaacggacac ccttgacgtc
    14401 gatatttttg atatttggca tttgtctatc acaggtctat tgattatgtg gtatgcggct
    14461 cgcaaccatt taccagtctt tgtgtctctt ttgattgcta ctgtggcagt tattcttgtt
    14521 ggggtattga aaatcattaa ctctgatttg atgaaaccga cctttgatga ccttcttggg
    14581 acagggtctc aatcaccaat gacttcaact cacatgaact acatgatgaa tccaatcatt
    14641 atggtcttag ataagatttt tgacaaggtc tttccggggc tggataaata tgactttgat
    14701 gctgctaaat tgaacaaaaa aattggcttc tggggctcta agttctttat cggaatggct
    14761 ctaggttttg tgattgggat tatgggagat cctcatttca ctgttgaatc tatcaaaaat
    14821 tggtttggtc taggctttac cgcgggtgcc tgcttggaac ttttctcttt gattggttct
    14881 tggtttatcg cagctgttga gcctttgtca caagggatta ctaattttgc caatgctaga
    14941 atgcatggac gtcgtttcaa tatcggtttg gactggcctt tcattgctgg tcgcgctgaa
    15001 atctgggcct gtgccaatat tcttgcccca atcatgttga ttgaagccgt tctcctatca
    15061 aaagttggca atgggatttt accactggca ggaattattg ctatggggat gacaccagct
    15121 cttcttgtgg tcacacgtgg tcgtttgatt cgcatgatta tttttggttc cctcttattg
    15181 ccattattcc tcttgtcagg aactatgatt gctccatttg cgacagagtt ggctaaaaaa
    15241 gtaggggctt ttccagcagg tacaagcgct ggttccttga ttacgcattc aacacttgaa
    15301 ggaccaatgg aaaaaatttt tggttatgtg attggtcaag cgacaacagg tcagattgcc
    15361 tcaatcatca cgctaatcat ctttgtggcc atttacctag gtctctttgc ttggtatgct
    15421 aaccaaatga aggcacgtaa cgctgagtat gcgaaaacaa tgaaataaaa tcaggtcagg
    15481 tgactgacta aaaaattagg gagaatagca gataactgcg taaggtattt ctgccttacc
    15541 ccctaacgtg aaaagagtat ttctcatgta aggcaaggcg ctctgtggga actcacaagt
    15601 gctttgtctt gaaagaaata atggaagaca ctaagactac tggcaatcat cttgatctaa
    15661 ggacaggtaa tcatgtcccc cttctctaga ttggcaagcg ttttgggtta gaaaaggaaa
    15721 agctatggct attattttag gtgcagatgc acacggaaat gcattaaaag aattgatcaa
    15781 aagttttctt caagaagaag gctatgacat tattgacgtg actgatatta acagtgattt
    15841 tattgataac acattagcgg tggcgaaagc cgttaatgaa gcagaaggtc gtttaggcat
    15901 tatggtggat gcttacggag caggtccctt tatggtagcc acaaaattaa aaggaatggt
    15961 tgctgctgaa gtgtcagatg aacgctctgc ttacatgacg cgcggtcaca acaatgcccg
    16021 catgatcaca ataggggcag agattgtagg tccagaatta gcgaagaata tcgtcaaagg
    16081 ttttgtgact ggtccctacg atggtggtcg tcatcaaatt cgtgttgaca tgctcaataa
    16141 aatggcctaa aggaggataa agtgatgaaa attgcgattg gttgtgacca tattgtcacc
    16201 aacgaaaaaa tggctgtttc tgattttttg aaatctaaag gttatgacgt tattgactgt
    16261 ggcacttatg accatacaag aacccattac cctatttttg gaaaaaaagt cggcgaagct
    16321 gtggttagtg gtcaagcaga cttgggtgtt tgtatttgtg gaactggagt tggcattaat
    16381 aatgccgtga acaaggtacc aggtattcgt tcagcccttg ttagagatat gacaacagcg
    16441 ctttatgcca aggaagaatt gaatgccaat gtgattggct ttggtggtaa aatcactggt
    16501 gaacttctta tgtgtgatat tatagatgct tttatcaagg cagaatacaa agaaactgaa
    16561 gagaataaga agctaattgc taaaatagcc cacttggaaa gtcatcatgc caaccaagaa
    16621 gatcctgatt tctttacaga atttttagaa aaatgggacc gtggtgagta tcacgattag
    16681 gaagaagaga tgagtttaac agttatctta aatccttctg tcgctatttc ctatccttta
    16741 gattgtctgg ctatggacac ggttaatcgt gttgacagaa cggcaaaaac agctggtgat
    16801 aaggagctaa atgtgaccaa aagctggtca gttatggcaa caggatttag tggtggtaaa
    16861 ctgggtgact ttatcattca tcagttacaa gaacagggca tttctaacca atttttcaaa
    16921 atcaaagggg aaacgagaaa ttgtattgct gttttacatg aaggcatgca gacaaaaatt
    16981 ttggaagctg gtccctacag tgatgtggac gaagccgaag tctctttgag ccacatgagt
    17041 actattgcta agccgtttga tgtcttaact tctcaggcag tctgcctaag agactagcta
    17101 ctcactatta tcaagatttg atgactatgg caacttttaa acaattatat cagcaacttc
    17161 aagtaagaga ggtatcacat gacaatcaca gcaaataaaa gacattactt agaaaaagtg
    17221 agccatcaag gaatcatatc agctttagct tttgatcaac gaggtgcact gaagcaaatg
    17281 atggcagcac atcaagaagg agaagcgaca gttacacaga tagaaacatt gaaagttctc
    17341 gtttctgagg aattgacgcc ttatgcttca tctattttat tagatccaga atatgggttg
    17401 ctggctacta aggtaagagc taatcagaca ggcttactgt tagcttacga aaaaacaggt
    17461 tatgatgcta caaccactag ccgtttaccc gattgcttag tggaatggtc ggtcaaacgg
    17521 ttgaaagcag caggtgcaga tgctattaaa tttttgctct attatgatgt tgatggagat
    17581 gaacaaatca atttacaaaa acaagcttat attgaacgca ttggttcaga atgtacagct
    17641 gaagacatcc ctttttttct tgaacttttg agttatgatg agagaatatc agataacaac
    17701 agtgcggcat acgcaaagct taagccacat aaggttaacg gtgctatgtc agtattttcg
    17761 gataagcgtt ttggcgttga tgtgctaaaa gtagaggttc ctgttaatat ggcttatgta
    17821 gaaggtttta ctgaagggga agtccattac agtcaagctg aggctattaa agcttttcaa
    17881 gaccaagaag ctgccagcca cctcccttat atctatttga gtgcaggcgt atctgccaaa
    17941 ctttttcaag agacattata ctttgcagca gcagcaggtg ctcaatttag cggtgttttg
    18001 tgtgggcgtg ccacgtgggc aggatccgtt ccagtttata ttactaaagg agaggacgaa
    18061 gcaagaaagt ggctatgcac tgaaggcttc caaaatattg atgagttaaa cagggtactc
    18121 gaagagaccg ctagcccttg gacggaaaaa atctaaaaca accttatcct atcaagcagc
    18181 tctcattgat gtgataagat aagtatgggg ataattgttt gaatcttttg tagcaaggat
    18241 gaaaatgggt tttatccttg cttttttatc cttacatttt atctttttct acttattttt
    18301 taaaggaaaa cgtggtaaac taaaagaaga ctaaaagaca atagggtagc ttatgacaat
    18361 tgatatgtgg gaaattttaa atatcatcgg gaccattgcc tttgctcttt ctggtgctat
    18421 tgttgccatg gaagaagaat ttgatatttt agggattttt attttaggtt tcgtgacagc
    18481 ctttggaggc ggagccattc gaaacaccct aattggttta cctatagagg ccttatgggg
    18541 gcaaaagcca gaattcactt gcgctttctt tgctatggtt ttgattatgc tttttcctaa
    18601 gctaatagct agaggttggg tacgggcagc tgttttgact gatgccattg gtctggcagc
    18661 ttttagtgtc caaggagcct tgcatgctgt ccgtcttaac cagcctttaa gtgctgtcat
    18721 tgtagcagct gtgttgactg gtgcaggcgg tggtgttgtc agagatattc tggcagggcg
    18781 aaaacctagt gttttgcgta gtgaaatcta tgctgggtgg tctattcttg cagctattgt
    18841 tttgcatttt aaactagcag attcaacgat tgaatgttat gccttggttg ttttactcac
    18901 gactttacgc atgattggca accgtaaaaa atggaacctc cctaaaatca aatggaaaga
    18961 ggaaagatag gatgattcaa ctaattgcaa ttgatttgga cggcacctta ttaaaccagg
    19021 acaaacagat tccaaaagag aatatcacag ccatccaaga agcagctcag tcaggcttaa
    19081 aaattgtctt atgcacaggc cggcctcagt cagggacaag accctatttt gaccaattag
    19141 gtttgacaca agaggaattc ctcattatca ataatggctg tagtacttat agtagtcctg
    19201 actggcaatt acgccattca aaaatgttga aggtttcaga tattgaattg ctggaagagc
    19261 taagccaaag ttttccagat atttacctga ctttaactga ggaaaacgat tatttggtgc
    19321 ttgaagaaga agtaccagac cttgtgcagg aagatggaga tttggtattt accattgtca
    19381 agcctgtctc cttagcagag ctgtcggaca ctcctcgctt gatttttcaa gccatgtact
    19441 taggagaaaa agctgcgcta gatgcctttg aacgtgcggt gagaaatcag cttagtcaga
    19501 gttttcatgt tgttcgtagc caagataata ttttagaaat tctccctcag ggagtcagca
    19561 aggcatcggc tctcaaagaa ttagtagaag atttgggttt aactgctgac caagtgatgg
    19621 ctattgggga tgctcctaat gacattgaga tgttaactta tgctggttta ggagttgcta
    19681 tggaaaatgc ttcagcagct atcaagccat tagctgacaa agtcactttg actaatgata
    19741 tggcgggtgt ggcccaggct atccgtcagt ttgcacttgc agctcaaaaa gactaactat
    19801 tatataaaaa aggttcccaa aaggaacctt tttacgttaa tagaggcaag cccatagcag
    19861 ccatttcttc ggcaggaacg atcatgtcat ctgaaatcca gtttgtcaag acagaaacaa
    19921 tagcattgct ccaaaaaagg cgcttatagt gcgattgttt tttgtctttt aatttttgat
    19981 aagctttcag acgtttagtg actaatcgtg ttagaagatg gtgcaggtga tgtttgaaaa
    20041 tcattttaag aagctgagct tcttttttag cttcagtaaa aagatagagc caggcttgat
    20101 aagcctgagt tttaatatca aattgtttta gtccacgaaa aatacgacgg ataaccccta
    20161 ctaacagttg ttctataatg gcttctttag aggtataatt acgataaaaa gcgttacgag
    20221 atactcctgc tttcgtcacc aattctgaaa tcgtaatatc acctaaaggt tttgtttcta
    20281 gaagatttag taaggcgatt tccatagatt cacgtgctat gcgtcgtgtt tctttattaa
    20341 attgtgttaa gttttgaagt gatctttttg agagtgcttt atcagtcatc acaaatacct
    20401 ttcaagtgtt acatctgatg agttatcagt agcaaaaacc gacttaataa tgatataatt
    20461 ttatgggaaa agtcttagtt tgtcaactaa agttagttat caggagcctc attacctcct
    20521 tgtatagctc tattggatga ctttactaac tattgaaaag gctagtaata aaacatagga
    20581 tttaatgact agttattacc attagcctag gcatcttagc gaaggcttaa taatctggtc
    20641 tttgttaccg aacacaaatt agagaaaaag aaggataaaa aatgacttgg aaaattgtaa
    20701 cagattcagg atgtgatctt agaagtttga ctagacaatc caaagagttg cgctttgaac
    20761 gtgtgccttt gaccctacag attggcacag aaatttttag agatgatgat ggattagata
    20821 tcgataatat gatgacaact atgtaccagt cgtctaaagc aacgacatcc agctgtccaa
    20881 gcccagaagc ctttttgcag gcctatagag gtgcagataa tgttatcgtg atgacaatta
    20941 cagggacctt atctggcagt cataatagtg cacgactggc taaaaatgaa cttcttgaag
    21001 aaaatcctaa tgtcaatatt cacttaatcg attctttatc agcaggtggc gagatggatt
    21061 tgctcgtact ggagcttgag cggttgatca atcttggatt atcctttgaa gaagttgtca
    21121 aacagatcac agcttatcag caaaaaacac gtttgatttt tgtgcttgcc aaggttgata
    21181 atttggttaa aaatggtcga ctgagtaaat tagtaggtaa agtaattggc ttactcaata
    21241 ttagaatggt tgggaaagct agcaataaag gaacgcttga acttcttcaa aaagcgcgtg
    21301 gtcaaaaaaa agcagtttct gctttgatag aagaaattca aaaagagggt tatgtaggcg
    21361 gaaaagttta cattgctcat gctcaaaatc caaaaatttg tgagcagatc agtgaaaaaa
    21421 tcaaatcttt atatcctgat gctgtgattc aaactggaag gacctcaggt ttgtgtagct
    21481 tttatgctga agacggagga cttttaatgg gctacgaaat ttaatgctat ctaacaaaac
    21541 aaaaagctag actcttttga tttgagctag ctttttgttt tgttaggagg actaatcact
    21601 aagatagccg tctaaaacgt ttattgattg attttacaaa aaatttttgt attactcttt
    21661 tgatgattgc ttgtctcctg agttggaagg ttctgaagag gtctgaggtg gaattggatc
    21721 aggatgacta ggttgtggcg taacatctga agctgaagat tctgagcttg ggtctaaagt
    21781 aggtgactgg gaattatcat aagtatctga gacgtctcct gggaaaggtg tccagtaaga
    21841 gttatctgtt atttctccac tttgtcttgg aaattcatca ataatgggtt tcgctggtaa
    21901 aatagcgtct aaccgagatt gtaaagcggc ttttttacct ttgttactta agtcgttaac
    21961 tgctttttgg gcgatatctt taagattttg attgagagtc tcctcagcag ctttaactgc
    22021 ttgagtagct gcatcttctt gctcttgctt tgctttggca acgtttagct gttcttgtag
    22081 cgtttttttc ttagaagatt ctgctaattt atcaacagct ttctgggctt cttggatagc
    22141 agctgcagtg gctaaacgat cagcgtcatt aactgcttta actgcttttt ttagtaaact
    22201 agcagcactt ttagaatgtt tagctacctt atcttgcgtt tgagggtggg atccaaaaat
    22261 aaggcaggag ccacctatta gagtgatagt tagaacactc aacatccaat aaagcgcctc
    22321 tttttgtgat ttagtcatgg aatctccttt atgatattgt ttcatcgtag ccatattata
    22381 cctctaatta aacgaaatga aaagatttgt gcaaaaacaa atgatgtaaa atatcggaaa
    22441 agcctaacac cttatcaaac caataatatg caaatgttga catcggtata cacagaaacg
    22501 cttaaaaaaa ccttgtgata aagactgtaa tacattaaaa tagaatagac taaaaaattg
    22561 agcgaggaca atattgtgtc agtaaattgg caagacattg catttcattt ctttggaggc
    22621 ttaggtctct ttcttttcag tatcaagtat atgggagatg gtctgcaaca agcagcagga
    22681 gataaactaa ggtattacat tgataaatac accagtaatc ccttttttgg tatcttagtc
    22741 ggaattgcca tgtctgcctt aattcagtcc agttcaggcg tcactgtgat tacggttggt
    22801 ttggtttctg cgggtttgct aaatttgcga caagccattg gaattgttat gggggctaat
    22861 attggaacga ctatcacttc gtttttaatt ggttttaaac taggagatta cgccttgccc
    22921 atgattttta ttggtgcagc ctgtcttttc tttacctcta ataaaaagct caacaatttt
    22981 gggcgtatca tttttggtgt cggaggaatt ttcttctcgc tgaacctaat gggagatgcc
    23041 atggatcctt tgaaatctgt gtcagccttt caaaattatt tagcgacatt aggtgataaa
    23101 cccttccaag gtgtctttat cggaacggct ttgaccatgt tgattcagtc atcggcagcc
    23161 attatcggga ttttacaggg actgttttca ggcggtcttt tgaccttaca gggggctatt
    23221 cctattttgt taggctcaaa tataggaacc tgcattacag ctgtcttagc agctataggt
    23281 tcaaatattg ctgctaaacg tgtggcagca gctcatgtgt tatttaattt gatcggtaca
    23341 attattttta tgattatcct agtcccattc acatcattga tgttatggct acaatcaaag
    23401 ttgagtttaa cgcctgagat gactatcgct ttttctcacg gttcttttaa tatcactaat
    23461 accatcttat taattccttt cattagtcta ctagctatga ttgtgactag actcattcca
    23521 ggagaagatg aagtggtcaa atacgaagcc ctttatttag atcgcttact tattacccaa
    23581 gccccttcta tcgcactagg aaatgcccac aaggaattgg ttcatttagc ctcttatgcg
    23641 attcaagctt ttgaagcttc ttacagttat attatgacag ctgacgataa atttggggaa
    23701 aaggtcaagc gttatgaaag agcagttgat accatcgatg aggagttaac cacttatttg
    23761 gttgatattt ctaacgaagc cctcagccca agtgaaaatg aagtcttggc tggtattctt
    23821 gactcttcac gtgatttgga aagaattgga gatcattctg agtctttagg cattctaatt
    23881 gaaggcatca tatctaaaca aattggtttt tcaatatctg ctcgtcaaga attgacagag
    23941 atgtatcaat tgacgcattg tttaacactg gatgccatta gagctatcgt tgatagtgat
    24001 acagacttgg cgcaaactat tgtgactcgc cataaagaaa ttgaagaaaa agagcgacga
    24061 ttacgtaaaa cccacattaa gcgcttgaat tgtggagaat gtacggcaca agcaggtatt
    24121 aattttattg atattatttc ccactatacc cgcatcacag atcatgcctt gaatttagca
    24181 gagaaagtac tttcacacca gctttaaaaa aagagaaaac gtgaatgatc atccacgttt
    24241 tcttttttga atccaaaaag tggtatatac catttacaaa gtaaagaata aggcgtataa
    24301 taaggaaaaa agggaggaaa aaagatgaca tgctatctta aagcagattg tttttattac
    24361 ccaacagagg tgaaaccggc tggctaccta agtcttcatg atggtgtttt tggggagtgg
    24421 acagaaattg ttccggcaga tgcgcaaatc attgactaca ccggttatca aattgcacca
    24481 ggtcttgtgg atacgcatat ccatggctat gcgggagctg atgtcatgga taattctgcc
    24541 caagggattc atcaaatgag tgaaggacta ttagcaacag gagtaacgtc ttttttaccg
    24601 acgaccttaa cttctacctt tgagcagtta gaaaaagtat cagggactat tgcttcagtt
    24661 gctgatcagg taaaaggtgc taaaatacaa ggtatctact ttgaaggtcc ttattttacc
    24721 gaagaatata agggtgctca aaacccaagt tatatgaaaa ccccacgctt ggaggaattt
    24781 gatgcttggc aaaaagctgc taaagggctc atcaaaaaaa ttgcccttgc tcctgagcgt
    24841 gagggtgtca aagaatttgt gtctgcagtg acaaaacaag gcgtaacagt ggccttaggg
    24901 cattcaaatg gtacttatca agaggctaaa gaagctgttc aagctggtgc tagtgtttgg
    24961 gttcatgctt ataatggcat gcgaggattg acccacagtg aaccaggaat ggtgggagcg
    25021 gtttataatc ttccaaatac ttacgcagag ttgatttgtg atggccacca tgtgtcacca
    25081 gttgcttgtg atattttgat gcagcaaaaa gggcatgatc atgtagccat gattacggac
    25141 tgtatgcgag caggtggttc accagatggt gattacctct tgggggaatt ctctgtggtt
    25201 gtggcaaatg gcactgctcg tcttaaagag agtggaaact tggcaggatc tattttgaaa
    25261 ttaaaagacg gccttagaaa tgtagtggcc tggggaatcg caactccggc agaagccatt
    25321 catatggcaa cttatgtgcc agctgtttct gtcggtattg acgacgtatg tggtcaaatc
    25381 aaagctgggc atgcggctga ctttattgtt cttgataagg atttaacact tgttgcaacc
    25441 tatttaaatg ggcaaaaagc ttttgatgcc taagagatac aaaaggattg gggtagctat
    25501 gtctggcagg ccaattcttt ttgtattttc tttctttatg atagaataga ttaaagtaat
    25561 cgcttacaga tattgataaa aggaggtttt tgtgatggta acaacagtaa aaatgacaag
    25621 tggctatgag attcccgttt taggatttgg aacttaccaa gcagccgatg gtgaggaagc
    25681 ttaccaatct accttagcag ccatcaaggc tggctatcgt catattgata ctgcagctat
    25741 ttataaaaat gaagaaagtg tgggtcgtgc catcaaggat tctggggttc tacgagaaga
    25801 cttatttatt acaactaaac tctggaatga cgcccatagt tatgaagggg caaaagacgc
    25861 tttagctgct tctcttgacc gtcttggtct ggattatgtg gatctctatc ttatccattg
    25921 gccaaaccca aaagctctgc gtaacgcttg gaaagaggca aatgcccaag catggcaata
    25981 tatggaagag gcggttgaag cagggcttat taaaagcatt ggcgtcagca attttatggt
    26041 gcatcatcta gaagctcttc aagagacagc taaaataact ccagcaatta atcagattcg
    26101 attagcccca ggttgctatc aaaaggaagt ggttgattat tgtaaggcaa atgagatttt
    26161 attggaagct tggagtcctc taggacaagg ggagattttt gataatgaga ctatgcagca
    26221 gttagctaat aaatatgaca aaacagtggc acaagttgcc ttagcttggt cgctggcaga
    26281 aggttttatt cccttgccaa aatcggtgca tgatgagcgc atcaaggaaa atatggctat
    26341 ttttgatgtg agcttgacac aagaagataa aaaaacaatt cgctatctct caggaatgtc
    26401 agcgattcct aaccctgata ctacaagttt ttaaggaact acaaagattt agccataatt
    26461 cgctaaatct tttttgaaaa cggtttgaca aaggtttatc atttagctat aatctttatt
    26521 agtgttttgt gaaagaaact attattgaaa ataaggagcc gtattgtgac catcttttta
    26581 gtgatgttag ctatgtttac tattcgttta gtatttttga agaaatcgat aacaaatgaa
    26641 aaagccatat tagcccaagg aggacaggaa tttggtgctc agaacacgaa atttttgacg
    26701 ttacttcaca ttatgattta tgtgtttgcg gtgattgaag cgctgcttaa acaaattaag
    26761 tttgatggca ttagtttttt aggtcttttg ttgatgttat tatcagtagc tgttttatat
    26821 gaagtgacac gtatcttagg tgacatttgg actgtaaaat tgatgttagc aaaagaccac
    26881 aaatatgtgg atcactggtt gttcaaaacg attaaacatc ctaactactt tttaaatatc
    26941 gcccctgagc tggtgggcat agccttactt tgccatgcta aaattacggc tatgcttctt
    27001 ttcccatgct acattgtagt aatatattta cgtattcgag aagaaaacaa gctattagcg
    27061 gaagtgatta ttccaaacgg tactcgaaca aaaccttaag ctcatgatgg acaaactaat
    27121 ggctttatgc taaactagac tctaggaaaa aggatgcaag tatcttatct aaagcgagtt
    27181 cggggtagtt agagccgaat ggtaggactg cagattggcg cttccgtttg ggcagtgtga
    27241 ttaagtatat ttgtcaatat tgcccaaaaa gatactatat aaatgaagta ataaattagg
    27301 gtggaaccgc gttttgacgc ccctaggtct tatgatttag gagcatcaga atgcggtttt
    27361 ttggtgccta gacctgtaat acatgctagt aacatcgtca ggtattttac tgatgacaaa
    27421 aaaacgattg tcaatgatat accattttac tactatcaaa ggagtacgaa atgtctaaaa
    27481 aacttacctt tcaagaaatc atcttgacct tacaacaata ttggaatgat cagggttgta
    27541 tgctcatgca agcctatgac aatgaaaaag gagctgggac catgagtcct tacacgttct
    27601 tacgtgctat aggtcctgag ccatggaatg cggcttatgt agagccatca cgtcgtcctg
    27661 cagatggccg ttacggagaa aacccaaatc gtctttacca acaccaccaa tttcaagtgg
    27721 tcatgaaacc atcaccatca aatattcaag aactttacct tgcttctttg gaaaaattga
    27781 gcatcaatcc tttagaacat gatattcgtt ttgttgaaga taactgggaa aacccatcaa
    27841 caggatcagc agggcttggt tgggaagttt ggcttgatgg aatggaaatc acacaattta
    27901 cgtatttcca acaagttggt ggccttgcga catctccagt gacagcagaa gtaacttatg
    27961 gtcttgagcg cttagcttcc tatattcaag aagtggattc ggtctatgat attgagtggg
    28021 caccaggtgt taaatatggt gaaatcttct tacaacctga gtatgagcat tcaaaatatt
    28081 ccttcgaaat atcagaccaa gacatgctgc ttgaaaactt tgaaaagttt gaaaaagaag
    28141 cctcgcgtgc cctagaagaa ggtttggttc acccagctta tgattacgtg ctcaaatgtt
    28201 ctcatacgtt taacctgctg gatgcccgtg gcgctgtttc cgtcacagaa cgtgcaggct
    28261 acattgctcg tatccgtaat ttggcacgtg tcgttgctaa gacctttgtt gcagaacgca
    28321 agaagcttgg atttccactg ttggatgaag caacacgcgc aatcttgcta gctgaagatg
    28381 acgaataagt cgatttaaag atgatctagt tgcgagcttt gtaatcttaa gagcgagcaa
    28441 gaagtgggcg accgaaggtc gccgagtcaa ggttactccc gctgtagtga caattcggta
    28501 aggttactta tcgaacgagg gagttggcga gactttaggc tggctaacta ggaatggaac
    28561 acttttgttt tctgaaatta tccactggat aatttcactt tgcgtccccc ctacctaagg
    28621 gaacaatcaa aaagatactt tttagagaaa ggaactgaac acgggctacg gactatgtca
    28681 aaaagaccaa gacttcctag gtgcaagcac ctgcgtcgct ttgctatttt gatggtgtcc
    28741 gcttaacgcc cttggtatct taattatgag taaaaatcta ctaatcgaac ttggtctgga
    28801 agaacttcca gcctacgtgg tcacaccaag tgaaaaacaa ctgggagagc gcttggcgac
    28861 tttcttaaca gaaaatcgtc tctcttttga agatatacaa accttttcaa ctccacgccg
    28921 tttggcagtg cgtgtgtctg gattagctga tcagcaaacg gatttgactg aggactttaa
    28981 agggcctgct aagaaaattg cccttgatgc tgatgggaat ttctcaaaag ctgctcaagg
    29041 atttgttcgt ggcaaaggct tgaccacaga tgctatcgaa ttccgcgaag tcaaaggaga
    29101 agaatacgtt tatgtgacca aacacgaagc tggaaaacct gctaaggaag tgcttctagg
    29161 cgttactgaa gtgttgtcag caatgacctt cccagtcagc atgcattggg ctaacaatag
    29221 ttttgaatac attcgtcctg ttcacacctt gacagttctg ttgaatgatg aggccttgga
    29281 acttgacttc ttggatattc attctggacg cgtcagccgt ggtcaccgtt ttttaggtac
    29341 tgaaaccact atcacaagtg ctgattctta tgaggctgat ttgcgcagcc agtttgttat
    29401 cgctgatgct aaagagcgtc aagagatgat tgtggagcaa atcaagacgc ttgaagtgga
    29461 gcagggtgtg caagttgaca ttgacgaaga cttgcttaat gaagttttga acttggtcga
    29521 gtttccaaca gctttcatgg gaagttttga ggctaaatac cttgatgtcc cagaagaagt
    29581 attggtaaca tcaatgaaaa accaccaacg ttattttgta gtacgtgatc aggctggtca
    29641 cttgatgcca aactttgtat cagttcgtaa tgggaatgat caagccattg aaaatgttat
    29701 taaagggaac gaaaaagttt tggtagctcg cttagaagat ggtgaattct tctggcgtga
    29761 ggatcagaaa ttgcaaattg ctgatttggt tgccaaattg accaatgtga ccttccatga
    29821 aaaaatcggc tctcttgctg aacacatgga tcgtacgcgt gtcattgccg catcacttgc
    29881 taaggaagcc aacctatctg ctgaggaagt cacagctgtt gaccgtgctg cccaaattta
    29941 caaatttgac ctgttaacag gtatggtggg tgaatttgat gagctgcaag ggattatggg
    30001 tgaaaaatat gcccttctag ctggtgaaga tgctgctgtg gcaacagcaa tccgtgaaca
    30061 ctacttacct gatgctgcag gaggtgtgct acctgaaacc aaagttggcg ctgtcctggc
    30121 cttggcagat aaattggata ctctcttgtc attcttttct gttggcttga ttccatctgg
    30181 ctctaatgat ccttatgctc tacgtcgtgc aacccaaggg attgtccgta ttcttgatca
    30241 ttttggatgg tgcatcccaa tggataagct ggtagatagc ttatatgact tgtcctttga
    30301 tagtttgacc tatgccaata aggcggatgt gatgaacttt atccgtgctc gtgtggataa
    30361 gatgatgggc aaagccgcac caaaagacat tcgggaagct attctagcaa gttcaacctt
    30421 tgtggttcca gaaatgttgg ccgcagctga agctcttgtc aaagccagcc atacggaaaa
    30481 ttacaaacca gcagttgaat ctttgtcacg tgcttttaat ttggcagaaa aagcggacgc
    30541 ttctgttcag gtggacccta gcctctttga aaatgagcaa gaaaacactt tgtttgcagc
    30601 tattcaaggc ttgactctag caggcagtgc tgcccagcag ttagaacagg tctttgccct
    30661 tagcccagtg attaatgatt tctttgataa cacaatggtt atggcagaag atcaagccct
    30721 taagaacaac cgtttagcta ttttatcaga tttagtaagc aaagctaaga ccattgctgc
    30781 ttttaaccaa ctaaacacca agtaaataga aaggaaagag ccgttgggga cttagctaga
    30841 aaagtgagtt cagtacccga gtggaaaaaa caaggccttt aaacattgct tttcacagtg
    30901 agagtcaagt cagtttttta agccttatac ttttttccca ggctcttagt ggcttaacat
    30961 ggattctaaa aaaattgctc gtatcaatga gctggctaaa aagaaaaaaa cggtaggctt
    31021 gactggacct gaaaaagtgg aacaggcaaa actccgtgaa gaatacattg aaggctatcg
    31081 tcgctctgtc agacaccata tcgaaggcat caaattggtg gacgaagaag gaaacgacgt
    31141 gacccctgaa aaattaagac aggtgcaacg tgaaaaaggc ttgcacggcc gttcattaga
    31201 tgacccaaaa tcataagaca aaaagcagct ctgaactgct ttttgtttat cgtgatgact
    31261 gttagctttt attttaaatg gtatgatact tcaaagtgac cacacgtttt tatattgtat
    31321 agtcaaaata ttaggaaaaa ccatgtgttt gagttataat agaagaaatc aaagaaagaa
    31381 ggtagtactt atgacaatta aaaaattctc tctactagcc atagccagcc tctctttact
    31441 cagtcttgca gcctgtgata tggatgataa agacgatcat atggataatc aaccaaagac
    31501 aagtcaaact agcaagaaag ttaaactttc agaagacaaa gccaaatcaa ttgcgttaaa
    31561 agatgcttct gttacggaag ctgatgctca aatgctatca gttacacaag ataatgaaga
    31621 tggcaaagcc gtgtatgaaa tcgagtttca aaacaaagat caagaataca gctataccat
    31681 tgacgccaac tcaggggata ttgtggaaaa atcttctgaa ccaatcaatg actaatcaag
    31741 aactgtaatg aataagccag ccttaactct tgtgtccaat tgtctttgcc tcttaggcat
    31801 ggctgtttga taccgtgttg cctttgaggc ttagttctct ttatgaccca atgatgactc
    31861 tggattagca gagtgattgt tgggtctttt gattttcttt gataagaaag attacctcgt
    31921 tagaaatgag gagacgtggt tgtcatcagt ggatttttca agatcatcta gtgggataag
    31981 ctacctatct tatcagcttt tttttagctt taaaaattct tgataatata tttctgataa
    32041 aattagctat aatagaatca aataggaaaa cgcttactta aaggagggta atgaatgtcg
    32101 caagaaaagt atatcatggc tattgatcag ggaacaacaa gttcacgagc gattattttc
    32161 aatcaaaaag gggaaaaggt tagcagtagc caaaaagaat tccctcagat tttcccacac
    32221 gctggttggg tagagcacaa tgctaatcag atttggaatt cggttcagtc tgttattgcg
    32281 ggtgctttta ttgagtccag catcaaacca agccaaattg aagcaatcgg aattaccaac
    32341 caacgtgaga ccacagtggt ttgggacaaa aaaacgggtg tacctattta taatgctatt
    32401 gtgtggcaat cacggcagac ggcaccaatt gctgagcaac tcaaacaaga tggccataca
    32461 aaaatgatcc atgaaaagac aggccttgtt attgatgctt acttttctgc aaccaagatc
    32521 cgttggattt tggaccatgt tcctggtgct caagaacgag ctgaaaaagg cgagttactt
    32581 tttggaacca ttgatacttg gctggtttgg aaattgacag atggtgctgt tcatgtgacg
    32641 gattattcca atgcggctcg taccatgctc tataatatta aagacttaac atgggatgat
    32701 gagattttag agcttttaaa tattccaaaa gatatgcttc cagaggttaa atccaattct
    32761 gaaatttatg gcaaaacagc tgcctttcat ttttatgggg gcgaagtgcc tatttcaggt
    32821 atggcgggtg accagcaggc agctttgttt ggccaactag cttttgagcc tggtatggtg
    32881 aaaaacactt atggcaccgg ttctttcatt atcatgaata ctggtgatga gatgcagtta
    32941 tcgagtaata atcttttgac caccattggt tacgggatta atgggaaggt tcactatgcc
    33001 ttggaaggtt ccatctttat tgcaggctct gccattcaat ggttgcgaga tggtcttaaa
    33061 atgattgaga cttcaccaga atctgaacag ttcgctctag catcaactag tgacgatgaa
    33121 gtttacgttg tgcctgcttt taccggttta ggagctcctt actgggattc taatgcgcgt
    33181 ggatctgtct ttggcttgac acgtggtacc agcaaagaag actttgtcaa agcaacctta
    33241 caatctatcg cctatcaagt gcgagatgtt attgatacca tgcaggtgga ttcaggtatt
    33301 gacattcaac agctccgtgt ggatggcggt gcagccatga ataatatgct catgcaattt
    33361 caggctgata ttttaggaat tgatattgcc cgtgctaaaa atttagaaac aacagctcta
    33421 ggagcagctt tcttagcagg gcttgctgtg gggtattggg aagatatgga tgctttgaaa
    33481 gagttaaatg ccacaggcca gctctttaag gctagcatga atgaatcgcg caaagaaaaa
    33541 ttatacaagg gttggaagcg cgctgttaaa gcaactcaag ttttcaccca agaagaagat
    33601 gcagatgacg atgcaaaata actagaggag gatggaatgg aattttcaag agaaacaaga
    33661 cgcttagctc tccaaaaaat gcaagaaaga gatttagatc tactgattat tgggggaggt
    33721 attacgggtg ctggtgtggc gcttcaggcg gcagctagtg gcctagatac gggtctgatt
    33781 gagatgcaag attttgcgca aggaacttct agccgttcaa ccaaattggt tcacgggggg
    33841 cttcgttact tgaaacaatt tgatgtggag gtggtttcag atacggtgtc agagcgggct
    33901 gtggtgcaac aaattgcacc acacattcca aaaccagacc ctatgttatt acctgtttat
    33961 gacgaacctg gcagcacctt tagcatgttc cgtttgaagg tcgctatgga tttgtatgat
    34021 cttttagcag gcgtgtccaa tacgccagcg gccaacaagg tgttaaccaa agaagaagtc
    34081 ttaaaacgag aaccagactt aaaacaagaa ggcttgcttg gtggtggggt ttaccttgat
    34141 ttccgtaata atgatgcaag gcttgttatt gaaaatatca aacgagctaa tcgtgatggg
    34201 gctcttattg ctagtcatgt gaaagcagaa gatttcttgc tagatgacaa tggtaaaatt
    34261 atcggtgtta aggcgcgtga tttattaaca gatcaagaaa tcatcatcaa ggctaaattg
    34321 gtcatcaata ccacaggacc atggagtgat gagattcgtc aattctctca taagggacaa
    34381 ccgattcatc aaatgcgccc tacaaaaggg gtgcatctgg tagtggaccg tcaaaaatta
    34441 ccagtgtctc aacctgtcta tgttgacaca gggttaaatg atggtcgtat ggtctttgtc
    34501 ttgccacgtg aagaaaaaac ttattttgga acaacggaca cggactacac tggagacttg
    34561 gagcacccac aagttactca agaagatgtg gattatttgt taggcgttgt caataaccgc
    34621 tttccaaatg ccaacgtgac cattgatgat attgaaagca gttgggctgg tcttcgcccc
    34681 ttgttatcag gtaatagtgc ttctgactac aatggtggca acagcggtaa agtcagtgat
    34741 gatagttttg atcacttggt tgatactgtc aaagcctata ttaaccacga agatagccga
    34801 gaagctgttg aaaaagctat taagcaggtt gaaaccagca catctgaaaa agaattggat
    34861 ccgtctgcag tgtcacgagg ttcaagtttt gaccgtgatg agaatggact ctttaccttg
    34921 gcaggtggta agattaccga ctatcgcaaa atggctgaag gagcattgac agggattatt
    34981 caaaccctca aagaagagtt tggcaaatcc ttcaagctta tcaattcaaa aacctatcct
    35041 gtttcaggag gtgaaatcaa tccagccaat gtagattcag aaatagaagc ctatgctcaa
    35101 ttaggaactc ttagtggtct ttcgatggat gatgctaggt atttggcaaa cctttatggt
    35161 tctaatgcgc caaaagtctt tgccttaact cgtcaattaa cagcagctga agggttaagt
    35221 ttagctgaaa ccttgtcctt acattatgcg atggattatg aaatggccct taaaccgaca
    35281 gattatttct tgagaagaac aaatcacctc ttatttatgc gagatagcct agatgctttg
    35341 attgacccag tgattaatga aatggctaaa cattttgaat ggtctgatca ggaaagagtg
    35401 gcacaagaag acgatcttcg tcgcgtgatt gcagacaatg atttgagtgc cttaaaaggc
    35461 catcaggagg gttaatatgg atatctttgg cgaatttttg ggtacagcct tattggtttt
    35521 actaggaaat ggtgtggtag caggggttgt cttgcccaaa acgaaaaccc atgcttctgg
    35581 ctggatcgtg attgcgactg gctggggaat tgcagttgct gtggcagtct ttatcagtgg
    35641 caaagttgcc cctgcccacc tcaatccagc tgttagcctt gcttttgcca tgagtggaac
    35701 cattgcctgg tcaacagcca ttgcctatag ccttgcccaa ctattgggag ctatggttgg
    35761 ttcaactctg gtattccttc agttcagacc acattatctg gctgctgaga gtcaggctga
    35821 tattttaggg acatttgcga caggtcctgc tattcgagat actagctcaa acctattgag
    35881 tgaaatcttt gggacctttg tcttgatgct tggtattttg gcatttggct tatatgatat
    35941 gccagcagga ctaggaaccc tctgtgtagg taccttggtt attgggattg ggttatcctt
    36001 aggaggaacg acaggttacg ccattaaccc agcgcgtgat ttaggacctc gtctagttca
    36061 tgctattcta ccactcaaca acaagggaga ttctgattgg tcttatgctt ggatacctgt
    36121 tgtaggacca attattggag cggccttagc agttttgctt ttccaagtca tgtcctaacc
    36181 atcactaaag gggttggcta tcataggcca gctcctttgc attttcttta taggattagg
    36241 taaaacgctt tcatatctta aaaacaactg ctataataat actattatga cattaacgat
    36301 aaagaccata catgatatcg gggcatcctt tgctggtcta gcctttgttg ataaatataa
    36361 ggacctcaat ccagattccc aaatcattct cattgataag gaaagctgtc ccaattatat
    36421 tccaaatggt atcaaccaat tattcagagg agacattcaa gacctgtctg atgctatgtg
    36481 gggaagagcc tgcctagcag cacaaataga gtctaaccat cggtttattc aagcagaagt
    36541 gttagctatc gaagctccct caaacacctt gcttctcaaa gacagtcaag gacgagtttt
    36601 tgaagaaggt tatgaaacgc ttgtttgtgc catgggtgct agcccccagt ctcattatat
    36661 tgagacgtca cagaccaaca aagttttggt aaccaaatat tatgaagaaa gccaagctag
    36721 tttaaaactg attgaggcta gccaagaggt cttggttatt ggtgccggct tgattggctt
    36781 agaccttgcc tacagtttgt ccttacaagg caaacgggtc aaactgattg aagcagccga
    36841 acgtccagat ttctatcaga ctgacgcaga actgatagcc cctgtaatgg cagaaatgtc
    36901 cactcaccat gtcaccttta ttaataataa gcgtgttacg gctatccatg agatagaggg
    36961 aaaagttgtc gctcataccg agcaagggga caccttccaa ggtgatttgg ctattttagc
    37021 cattaacttt aggccaaata cacacctttt gcaaggacaa gtggcttgtg ctctggataa
    37081 gaccattttg gtcaatgaaa acttacagac cagccagtct aatatctatg ccattggaga
    37141 catggtttca ttgcattttg gtatattggg aatggattac tatacacctt tgattaacca
    37201 agcaatgaag acaggtcagg ctctggctct gcatttagct ggatatccta ttcctccatt
    37261 acagacggtc aaggtattag gaagttctca ctttggttat tatcgcgcga gtgttggtgt
    37321 gacagaagaa gaagcagagc tttatatgga tacgtgtagc tatttgtacc aaaatgggga
    37381 ttctaaaaac ctcttttggc tgaaattaat agctagaaaa acggatggga ttttaatagg
    37441 cgctcagctc ctatccaaaa ccaatgctct tgtcatcgcc aatcaattag gtcaggctct
    37501 ggctctgaaa gttacagacg ctgacttagc atttcaagat tttctctttt tacaaggtca
    37561 cagtgatttg gcttatcatc ttcatgaagc ttgtctcaaa ttatttgaaa agaggctccg
    37621 ccatgaagat tgaagactta atggataagg aacgaagggc ccaatatcgt ctcttggtaa
    37681 ccctttatca tgctaaagaa acgttaaggc taaaagacct aatgaggcta tcaaacttgt
    37741 ctaaagtgac cctattaaaa tacattgaca acctaaatca tttgtgtcga gaacaaggtt
    37801 tagcctgtca attactttta gaaaaagaca gtctgagctt aaaagaaaat ggccagttcc
    37861 attgggagga tttggtagct ctgcttctca aagaatcggt tgcctatcag atattgacct
    37921 acatgtactg ccatgaacac tttaacatca ccaacttatc cgttgaactg atggttagtg
    37981 aagctactct aaacagacaa ctagctcacc tcaaccagct tttatcagaa tttgatctgg
    38041 cgctctcgca aggcagacaa ctgggaagtg agcttcagtg gcgttatttc tattttgaat
    38101 tgttccgcca taccttgacc agacaaggca tagacgcctt ggtcaatcag ttagacgctt
    38161 cgcatttggc taccttaatt gagcggctga ttggccagtc attatcggca gaagccttag
    38221 aacaattatt gatttggttg gcgatttcgc aagcgcgaat gtcctttcaa aaaggctaca
    38281 atgatcattt tttaagagat agtgacttta tgacatctaa tattttcttt aagcgcttag
    38341 aaagcatgct tttacattac ttaagacgct atgcccttga atttgatgct tttgaagcta
    38401 agagcctctt tgtctttttg catgcttatc ctctacttcc tattgccagc atgaagtata
    38461 gtttgggttt tggaggtcct attgctgatc acatttcaga agccttatgg cttttaaaga
    38521 aggctcatgt gattatccat cagacaaagg aggaaatcat ctatggcttg gggatatttt
    38581 tttccaaagc ttactttttt aaaggagcta tccttagtca accaaccaat agccaatacc
    38641 tttatcaatt agtaggcgaa gataaaagag cgcttttaag ggttatcata aaccatttag
    38701 tgctgcaaat ggatcaggaa acggatttca gtcagcagtt gagtgacgac attttagcat
    38761 tgttgatttt ttccattgag cgtcaccatg agcctctttt agtagggctt gccttggggc
    38821 aaaataaggt ggaagcagcc attgcggaat tggccattag acgtcatttg ggtcatcgta
    38881 ggaattttca attaatgccc tatgatcatc agaaagttta tgattgtctg attacctatc
    38941 agactgtctg tttaccgaga caagatttac cctattaccg gttaaaacag tatagctccc
    39001 cctatgaact cactgcttta gaagcttttc tcaaagacct tttccagcaa aaaaatacac
    39061 gagaagagga actctcactc agcccagcag ctaagtctag ttttgcacac aaaactgtgt
    39121 gatacttggt cttttctaag gactgttaca cttttataat aataaagtag gatagtctaa
    39181 aggtagatga tttgaaaaag cttatcaaat agagcttaac aaaatcgaaa atttttggag
    39241 gtaagaaatg aaatttttcc tagatacagc taatgtagcc gctattaaag cgattaatga
    39301 acttggcgtt gtagatggtg tcaccaccaa tccaaccatt atctcacgtg aaggacgtga
    39361 ttttgaaaca gttatcaaag aaatctgtga catcgtcgat ggcccaatca gcgctgaagt
    39421 gacagggtta acagcagatg ctatggtaga agaagctaga agcattgcta agtggcatga
    39481 caatgtcgtc gttaaaattc caatgactac agaagggtta aaagcaacaa acattctttc
    39541 aaaagaaggg attaaaacta atgtgacctt gattttcaca gtaagccaag gtttaatggc
    39601 aatgaaagca ggcgcaactt acattagccc atttatcggt cgtttggaag atattggcac
    39661 agatgcttat caattaatca gcgaccttcg tgaaatcatt gacttgtacg atttccaagc
    39721 agaaatcatc gcagcaagta ttcgtacaac agcccatgtg gaagctgttg ctaaattagg
    39781 cgcacacatt gcgacgattc cagacccctt gtttgctaag atgacgcaac atcccttaac
    39841 gacaaatggt ttgaaaacct ttatggaaga ttgggcttcc tttaaaaaat aaggctaaag
    39901 agtctgtggc cactgtgtca acaggctctc ttatattcat cgtcaaaaag aattcaccaa
    39961 tgatgtccaa actagttttt ttctggcaaa ataaagaaaa agaatttagg gactttggag
    40021 gattttctga aaaaagcgtc tatttttgtg atacaataga caatagaaaa cgtttaattt
    40081 tagttgtaat aaacagagag gttttactta tgacatttga tgcaattgac cagttggcag
    40141 taaacactgt ccgcacccta tcaatggatg ccattcaagc ggcaaattct ggacacccag
    40201 gacttccaat gggggcagca cctatggcct atgttctttg gaatcacttc atgaatatca
    40261 atcccaaaac aagccgtaat tggtcaaaca gagaccgttt tatcctatca gcaggtcatg
    40321 gaagtgccat gctttatagc ttgttacact tagctggtta tgatttatct gtagaagatt
    40381 taaagaactt ccgtcaatgg ggttctaaaa caccaggtca cccagaagtg aaccacacag
    40441 acggtgtcga agcaaccaca ggacctcttg gtcaagggat cgcaaatgcc gttggtatgg
    40501 ccatggcaga agctcaccta gcagctaaat ttaacaaacc aggctttgac atcgttgatc
    40561 actacacatt tgctttgaat ggtgacggtg accttatgga aggggtcagc caagaagcag
    40621 caagtatggc aggacattta aaacttggga aattggtctt gctatatgat tcaaacgaca
    40681 tctctcttga tggtccaacc tctatggctt tcacagaaga tgtgaaagga cgtttcgaag
    40741 cttacggttg gcaacatatc cttgtgaaag acggaaacga tttagaagaa attgcagctg
    40801 ctattgaagc agctaaagct gaaactgaga aaccaaccat catcgaagtc aaaaccatta
    40861 ttggttttgg tgccgaaaaa caaggaactt cagctgttca cggcgctcct cttggagcag
    40921 aaggcattgc ttttgccaaa aaagcttacc aatggactca tcaagatttt gaagtgcctg
    40981 ctgaagtaac agagcgcttt gctcaaggtc ttcaagcgcg tggtgaaaaa gcagaacaag
    41041 cctggaatga cttgtttgca gcttatgaag cagaatatcc agaattagca gcagaatacc
    41101 aaaaagcctt tgctaacgaa gctgctcaag tagaacttga agcgcacgaa cttggtagct
    41161 caatggcgag tcgtgtatcc agtcaacaag ccatccaaca aatttcagaa caagtagcct
    41221 ctttctgggg tgggtcagca gacctttcag catctaacaa cactatggtt aaggctgaaa
    41281 cagatttcca accaggtcat tatgaaggcc gtaacatctg gtttggcgtt cgtgaatttg
    41341 ccatggctgc agccatgaac ggtattgccc ttcacgatgg aacacgcgtt tacggtggaa
    41401 ccttctttgt cttctcaaac tatcttcttc cagctgttcg tatggcagcg ctccaaagct
    41461 taccaacagt ttatgtcatg acacatgatt ctatcgctgt cggtgaggat gggccaacgc
    41521 acgaaccaat tgaacaattg gcaagtgttc gttcaatgcc taacttgaac gttattcgtc
    41581 cagcagacgg taacgaaaca aatgctgctt ggaaacgtgc cattgctgaa acagatcgcc
    41641 caaccatgct tgtattgacg cgtcaaaacc tcccagtgct tgagggcaca aaagaattgg
    41701 cagaagatgg tcttaacaaa ggagcttaca tcttgtcaga agccaaaggc gaccttgaag
    41761 gtatcttgat tgcaacaggt tcagaagtta aattagcttt agatactcaa gataaacttg
    41821 agtcagaagg cattcatgtt cgcgtcgtat ctatgccatc gcaaaatatc tttgacgaac
    41881 agtcagcaga gtataaagaa agcatcctac cagcagctgt gactaaacgt ctcgctatcg
    41941 aagcaggttc aagctttggc tgggcaaaat atgtcggcct agcaggaaaa acattgacca
    42001 ttgacacttg gggtgcttca gctccaggaa atcgtatctt cgaagaatat ggtttcactg
    42061 tggcaaatgc aactgagtta tacaaatcac tctaggttac acatcccaaa cagagtaaat
    42121 catttgattt actctgtttt tttaaaataa aatacccagt tctttctttt cctcaaactg
    42181 cctaatacac ttcttttgat aagaagtcat tgacaaaaca attcataaaa attataattt
    42241 aattgttatt gtattagcgt aaaagtacaa aaataaagga gtataaatgg aaaagaaacg
    42301 acagcgctta tacgatgtga ttagacaggc ttatgattat ccagaaaatc gtgaaaatgt
    42361 ggccttgtcg cagctacttt tagcggcttc taatagatta attaaacaca gtaacccact
    42421 tcttgttgcc taccaactaa accaagatgt ggataattac ttacttgata atgacatttt
    42481 acttccaaaa tcgctttgcc gttttaagca gtcattggaa aagtatattt tagaggaagc
    42541 ctcatcagtc tagggagaaa aagagaagaa aaggaaaaag gataccatga tagaatttaa
    42601 acatgtttca aagttgtatg gtgacaaaga agctctcagc gatttaaacg tcaccatcaa
    42661 tgacggtgaa atttttggcc ttatcggaca taatggtgca ggtaaaacca cgaccatcag
    42721 tattttgact tctattatcg aagcgagtta tggcgaagta tttgtggatg gtcaacttct
    42781 gacagaaaat agagaagcta tcaaaaaaca aattgcttat gtgccagatt ctcctgatat
    42841 tttcttgaat ctaactccaa atgagtattg gcagttttta gctaaaattt atggggtgtc
    42901 agatgaggac cgagaagaac ggttagcaca gctgacaaca ctttttgaac tgaaggaaga
    42961 agttaatcaa accattgata gtttctcaca tgggatgcgc caaaaagtga ttgtgattgg
    43021 tgctcttgta tcaaatccta acatctggat tttggatgaa ccattaacag gtttggatcc
    43081 tcaggcttct tttgacctta aggaaatgat gaaagctcat gcagcttctg gacatacagt
    43141 cttattttcg actcacgtgc tatcagtcgc tgagcagtta tgtgatcgta tcgggattct
    43201 aaaaaaaggg aaattgattt ttgttggaac gattgatgag ttgaaagaac accatcctga
    43261 caaagacttg gagagcatct accttgaact cgctgggcgt aaggcgcaag aagaggggtg
    43321 atggcatgaa ttggtctact atttgggaac ttatcaagat aaacattctt tattccaatc
    43381 cacaaagttt agctaacctc aaaaagcgtc aggaaaaaca cccgaaagaa aacttcaaag
    43441 cctataaaag catgatgagg caacaggcct taatgattgc catgtttttg gtgatttact
    43501 tttttatgtt tattggtgtc gatttcagtc attatccagg acttttctcc tttgacgttg
    43561 ctatgttttt tatcatgtca accttgacag cctttagctc tctttacacc attttttatg
    43621 aaagtaatga cctaaaactt tatattcact taccagtaac gtcagaagaa ctctatattg
    43681 caaaaattgt ctcgtcgcta gggatgggtg ctgtcttttt gatgcctctt atctctcttc
    43741 tcttaattgc ctactggcaa ctattgggaa atcctttgtc tattttagta gctattgtgc
    43801 tctttttagt gttactagtg agttcgatgg tattagctat ttatatcaat gcttgggtgg
    43861 gcaaaatcat tgtaagaagt cgcaaacgaa aactcatttc aactatcatg atgtttgtct
    43921 caacttttgg cgctttcgtc ttaatttttg cgattaatat tagtaacaac aagcgtacga
    43981 tgatggatgg cgtatttact gattacccaa ctattcccta tttcaaagga ttctatgatg
    44041 ttgttcaggt accgtttgct actgtggccc tccttaactt ttggttgcca ttgctactta
    44101 ttctagctat ggtatatggt attgtcacaa aagtgatgcc aacttattat cgtgaggctt
    44161 tttatattag taacgagaac aaagccaagc aaactaaaaa accagttaat cgtcctcatc
    44221 agaatcaatc actggcgcag ttgttgcgaa aacatcacct attaacgtta caaaatgcaa
    44281 ctttactgac acaaacctat ctcatgcccc tgatgtatgt gatgcttttt atcggtccaa
    44341 gtttgtcacg tggcacaggt ttctttaagc atatttcccc agattacttt ggggtggcct
    44401 tattatttgg ggttagtttg ggtgtcatgt gtgcaacacc aaccagcttt attggagtag
    44461 gtatttcact tgaaaaggat aactttacct ttattaaaag cttaccaata acgttaaaaa
    44521 aattcttgct ggataaattt tgcttgcttg taggtctgca actgattgtg cctatggtga
    44581 tttatcttgt gtttggtctc tttgtgttgc acctgcatcc tcttctaacg attgcttttt
    44641 gtcttggcta cgccctatca ctgattgtgc aaggggaatt gatgtatcgt cgtgactacc
    44701 ggcttctgga tttaaaatgg caagacatga cgcaactctt taccagagga gatgggcaat
    44761 ggttaacaat gggacttatc tttggtaatt taatagttgc aggtgtgcta ggttttggag
    44821 ctgttatcat tgccaatatc atccaacaac ctctgttgat tagtattcta ttgagttgtc
    44881 taatactaat ggttttaggt cttgctcaat tatggattca aaaaaccttc tggaaaagct
    44941 tagagaggct ttaacgaaga gggatcatta ttcggaaaaa cagtcctctg gggctgtttt
    45001 tatgctataa tttgggtatg atgaaacgac aatttgaaga tgtaacacgt atcgtaatta
    45061 aaattggcac tagttcatta gtgctaccaa caggtaaaat caatttagaa aaaatcgacc
    45121 aactggcttt tgtgatttca agtttgatga ataagggcaa ggaagttatt ttagtatcat
    45181 cgggagcgat gggatttgga ctagatattt tgaaaatgga aaaacgtccg accaatcttg
    45241 ctaaacaaca ggcagtttcc agtgttggtc aggtagccat gatgagtctt tattcacaaa
    45301 tttttgcaca ctaccaaacc aacgtgtcac aaattttatt gacgagagat gtggtggttt
    45361 tcccagaaag tctagccaat gttaccaatg cttttgaaag tctgattagt ttgggtattg
    45421 tgccgattgt taatgaaaac gatgcggtta gtgtggatga aatggatcat gcgactaaat
    45481 ttggagataa tgaccgttta tctgctgttg ttgctggtat tacaaaagct gatttattaa
    45541 tcatgttgtc tgatattgat ggcctttttg ataaaaaccc tactatttat gaggatgcgc
    45601 agctacgcag ccatgttgca gtcattacgc aagaaatcat tgcgtcagca ggtggtgcga
    45661 gcagtaaatt tggcacaggt gggatgctta gcaaaattca gtctgctcag atggtatttg
    45721 aaaacaaagg acagatggtc ttgatgaatg gagctaatcc cagagatatt ttaagagtgc
    45781 ttgaagggca accactaggc acctggttca agcagataga agaggtaagg catgactgat
    45841 ataacaatgt tagggcaaag ggcaaaacaa gctagtcttg caatagcacc tttgtcgacg
    45901 caaattaaaa atcgcttttt aagcacctta gcaaaggcat tagttgacga tacacagact
    45961 ctcttagccg ctaaccaaaa agatttagcg aatgctaaag aacatggtat ttctgacatc
    46021 atgatggatc gcttgcgctt aacttcagaa cggattaaag cgatagcaca gggagttcaa
    46081 caggttgctg acttagctga tccaatcgga caagtcatta agggctatac taacctcgat
    46141 ggccttaaaa tcctccaaaa gagagttcct ctaggggtga ttgcgatgat ttttgagagt
    46201 cgcccgaatg tttcagtgga tgcttttagt ctggcgttca aaaccaataa tgccatcatc
    46261 ctaaggggag gaaaagatgc ccttcactca aataaagctt tggtgaagtt gatccgacaa
    46321 agtctggaaa aatcagggat caccccagat gctgtccagc tagttgagga tccctcacat
    46381 gctgtcgctg aagaattgat gcaggcaacg gattatgtag atgttttgat tccacgtggt
    46441 ggtgctaaat taattcagac cgttaaggaa aaagcaaaag tgcctgtcat cgaaacaggt
    46501 gtggggaatg tgcatatcta tgtggatgct caagctgact tagatatggc tactaagatt
    46561 gttatcaacg ctaaaacaaa gcgcccaagt gtttgtaatg cagctgaggg attggtaatt
    46621 cacgaagctg tagcagcaag gtttattcct atgcttgaaa aagcgatcaa tcaggttcag
    46681 ccagtagaat ggcgggcaga tgacaaggct ttgccgttat ttgagcaggc agtgcctgca
    46741 aaagcagagg attttgagac cgaattttta gattatatca tgtctgttaa ggttgtttcc
    46801 tctttggaag aagctatttc ttggataaat cagcatacta gtcaccattc cgaagctatc
    46861 atcacaaggg atattaaggc agcagagacc tttcaagatt tagtggatgc tgctgctgtt
    46921 tatgtcaacg cgtcaacacg atttaccgat ggttttgttt ttgggcttgg tgctgaaatt
    46981 gggatttcta ctcaaaaaat acacgcccgt gggccaatgg gcttagaagc cttgaccagc
    47041 actaaatttt atattaatgg ggatggacat atcagagaat aagctactag aaggtccctt
    47101 aatgactaat gaaagagaaa aagaagaagc aaaggttact ttagaagaac ttttacatca
    47161 gattcaggtg attcatttgg aattgaaaat tgtccaaaat tgggactatt ttgttttttt
    47221 ggagatggta tgctggtcag ctggatcaaa cgtcgaaaaa tgagcaaggt taaccaatta
    47281 ttaatagact tataagttac tcttcaaaac tgccgaagat agctggcaga cgtcgatagg
    47341 ctattattag ctgacttacc gaatatgagt tgggataaaa cctttggtat ttggttagac
    47401 aacctgctga cagattgttg tgtgattaag gaagctaaaa atcttttaca agcagttgaa
    47461 actctagagg cagtggctaa gtcagtgctt gctaagttgg ctgatagcga acaaaaagaa
    47521 caagattaga gaagaggaag attcccacga gaatgaaatc cttttctctt actttttcat
    47581 ttctaaacct tttgaagtat ggtacaatta aagttatgac aaaagaattt catcacgtga
    47641 ccgtactcct tcacgaaaca gtggacatgc ttgacataaa gcctgatggg atttatgttg
    47701 atgcgacgct aggtggctca ggccactcag cttatttgtt gtccaaactt ggtgaagaag
    47761 ggcacctcta ttgttttgac caagaccaaa aggctattga caatgcacaa gttaccctca
    47821 aatcttatat tgacaaagga caggtaactt ttattaaaga taattttaga cacctcaaag
    47881 cacgtttaac agcgcttgga gttgatgaaa ttgatggtat cttatacgac cttggtgttt
    47941 ccagcccgca attggatgaa agagaacgag ggttttctta taaacaagat gctccattgg
    48001 atatgcgcat ggatcgtcag tcgctcttaa cagcttacga agtggtgaat acctatccat
    48061 tcaatgattt ggttaagatt tttttcaaat atggtgaaga taaattctcc aagcagattg
    48121 ctcgaaaaat tgaacaagca agagctatta agcctattga gacaacaaca gagttggcag
    48181 aattgattaa ggcagcaaag ccagctaaag agttgaagaa aaaaggccac cctgctaaac
    48241 agatttttca agctattcgc attgaagtca atgatgaatt gggagcggcc gatgaatcta
    48301 ttcaggacgc tatggaatta ttagcctttg atggtcgtat ctcagtcatt accttccatt
    48361 ctctggaaga tcgcctaacc aagcagttgt ttaaagaagc tagtacggtg gatgtgccaa
    48421 aagggcttcc tctaattcct gaagatatga aacctaagtt tgaacttgtt tcacgtaagc
    48481 cgatcttacc tagtcattca gagttaacag ctaataaaag ggcacactca gccaagctac
    48541 gtgttgccaa aaaaattcgg aaataagcct atgacaaatg aaaaaagaac tcaggcagtt
    48601 acaaatgcct tgcaaaaacg aataaaaact ttctctagaa ttgagaaggc tttttatact
    48661 gctatcattg taacagcgat tacgatggca gtaagtatta tttacctgca aagtcgtaag
    48721 ttgcagctac aacaagaaat cactagttta aatagtcata tttcggatca aaaactagag
    48781 cttaataatg ccaagcaaga agtaaatgag ttatctcgcc gtgatcgtat tatagatatt
    48841 gctggtaaag caggcttaag caaccgtaat aataatatca agaaagtcga gtaatctatg
    48901 aaaaaatggc aaaaatatgt tttagattat gttgtgcgcg ataggagaac tccagtcgaa
    48961 aatcgcgttc gagttggaca aaatatgatg ctcttaacta tctttatttt ctttattttc
    49021 attattaatt tcatgattat tattggaaca gatcaaaagt ttggagttag tttgtcagaa
    49081 ggggcgaaga aggtttatca agaaaccgtt acgatccaag ctaagcgtgg gaccatttat
    49141 gatcgaaatg gtacagctat tgcagtggat tctacgactt atagcatata cgcaattttg
    49201 gataaatcat ttgtctcggc ttcagatgaa aagttatatg tacaaccttc tcagtatgaa
    49261 acagtagctg atattttaaa aaagcatctg ggaatgaaaa aaacagatgt aattaaacag
    49321 cttaagcgta aaggactttt ccaagtctcg tttggaccgt cagggtctgg catttcatat
    49381 agtactatgt ctactattca aaaggctatg gaagatgcca aaatcaaggg aattgctttc
    49441 acaactagtc ctggtcgtat gtatccaaat gggacatttg cttcagaatt tataggctta
    49501 gcatctctaa cagaagataa aaagacaggt gttaagagtt tagttggaaa aacaggtcta
    49561 gaagcttctt ttgataaaat tttatcaggt caagatggtg ttattactta tcaaaaagat
    49621 cgaaatggga ccacactctt gggtacaggt aagactgtca agaaagctat tgatggcaaa
    49681 gatatttaca caacgctatc tgagcctatc cagaccttct tagaaaccca gatggatgtt
    49741 tttcaagcca aatcaaatgg tcagttggcc agtgcaacac ttgttaatgc taaaactggt
    49801 gaaattttgg caacaacaca acgccccact tataatgctg atactctgaa aggacttgaa
    49861 aatacgaact acaaatggta cagtgcactt catcaaggaa attttgagcc aggctcaacc
    49921 atgaaagtga tgactctggc agcggctatt gatgataaag ttttcaaccc aaacgaaacc
    49981 tttagcaatg ctaatggttt aacaattgca gatgctacta ttcaagactg gtctattaat
    50041 gaaggcattt ccacaggaca gtacatgaat tatgcacaag gatttgcctt ctcaagtaac
    50101 gttgggatga ctaaacttga acaaaaaatg ggtaatgcaa aatggatgaa ttatttgacg
    50161 aagttccgct ttggttttcc tactcgtttt ggtttaaaag atgaagacgc aggtatattt
    50221 ccttctgata atatcgtgac tcaagctatg agcgcttttg gtcaagggat ttctgtaacc
    50281 cagattcaaa tgcttagagc ctttactgct atttctaata atggtgagat gttagagcca
    50341 caatttatca gtcaaattta tgatcctaac acagcaagtt ttagaacggc aaataaagaa
    50401 attgttggaa aacctgtatc aaaaaaagcc gctagtgaaa caagacaata catgattggt
    50461 gtaggaacag accctgagtt tggaacactc tattcaaaaa catttggacc aattattaaa
    50521 gtgggtgatt tacctgttgc tgttaaatca ggaacagcac aaattggttc agaagatgga
    50581 agtggttatc aagatggtgg attgactaac tatgtctatt cggttgtggc aatggtgcca
    50641 gctgataaac cagacttttt gatgtatgtt actatgacta aaccacaaca ttttggtccc
    50701 cttttttggc aagatgtggt taacccagta ttggaagaag catacttaat gcaagataca
    50761 ctaactaagc cagtagtatc agatgctaat cgtcaaacaa cttataaatt accaaacttt
    50821 gtaggaaaga atcctggtga gacatcaagc gagttgcgtc gaaatcttgt ccagccagtt
    50881 gtccttggta ctggcagcaa gatcaaaaaa gtatcgcatc agcccggtca aacgttaaca
    50941 gaaaaccaac aagttctcat attatcagac cgttttgtgg aggtaccaga catgtatggc
    51001 tggacaaaat ccaatgttaa aacctttgct aaatggactg gaatagacat cagctttaaa
    51061 ggaacagatt ctggtcgtgt tatgaaacaa agtgttgatg ttggtaagtc cttgaaaaaa
    51121 ataaaaaaaa tgaccattac tttaggagat taacatgttt ttaaccctta ttgcagcaat
    51181 catatccttt atggtatctg ctttcacaat gccttatttt atcaaattct atcaattgaa
    51241 aaaaattggt gggcaacaaa tgcatgaaga tgtcaagcaa catttagcta aagctggaac
    51301 accgactatg gggggaactg tttttctttt agtagctacg gcagtatcgt tattggttag
    51361 tctattttcg atcaaaaaca ctcaaagttt agctttgatt tcaggcattt tatcaattgt
    51421 tgtcatttac gggattatcg gatttttgga tgatttttta aaaattttta agcaaatcaa
    51481 tgaaggattg acagccaaac aaaaattagc cttgcagttg gctggaggat tgatgttcta
    51541 ttttttacat gttagtccaa gtggcatttc ctctattaat gtgtttggtt atcagctgcc
    51601 gctgggaatt ttctacctat tctttgttct attctgggtg gttggtttct caaatgccgt
    51661 taatttgaca gacggtatcg atggcttagc ttctatctca gtggtgatta gtttagtgac
    51721 ttacggcgtt attgcctacg ttcaggggca atttgatgtc ttattattga ttggaaccat
    51781 gattggtgct ttacttggtt tcttcctatt taaccacaag cctgccaaag tatttatggg
    51841 agatgtgggg agtttggcct tgggagctat gttggcagct atttctattg cgcttcgtca
    51901 agaatggact ctcctgatta ttgggatcgt ttatgttctt gaaacaagtt ctgtgatatt
    51961 gcaagtgtcc tatttcaagt acaccaagaa aaaatatgga gaaggtcgtc gtatttttag
    52021 aatgacgcct ttccaccacc atttggaatt gggaggcttg tctggtaaag gcaagaagtg
    52081 gtcagaatgg caggtagatg cttttttgtg gggagtagga agtctagcta gtttactagt
    52141 tttagctatc ttatacgtct tctaaggggg attatcaaga atagtcacca atagcatttt
    52201 gattaacatc atcaataaga gagtggagat gacccacaaa tgctgatttt gtgagtgttg
    52261 tcccactcct tttgcttgtc tctaataata atgatataat atggaagaaa acgaggtaaa
    52321 acatgtcatt taaagattat cattttaagc aatacatcca gcaagcgctg gaagaaattg
    52381 gttttgtcaa tccaacagag gtccagaagc ggctgattcc tattgttaac tccggtcgtg
    52441 acttagttgg ggaatcaaaa acaggttcag ggaaaaccca cacctttttg ttgcccattt
    52501 ttgaaaaact tgatgaaact aaagcagaag tgcaagtcgt tattacagca ccaagccgtg
    52561 aattagcaac tcaaattttt gatgcttgta aacagattgc taagcactcc caagaagaga
    52621 ttcgtttggc caactatgtt ggaggcacag ataaactccg tcagattgaa aaactaaaag
    52681 gcagccagcc gcatattgtc atcgggaccc caggtcgtat ttatgacttg gtcaagtctg
    52741 gtgatttagc gattcataaa gcaacgacct ttgttgtgga tgaggccgat atgaccatgg
    52801 acatgggctt tttggatacg gtagataaga ttgcagcttc gcttccaaaa tctgttcaga
    52861 ttttggtgtt ctcagcaact attccacaga aattacaacc ctttttgaaa aaatacttga
    52921 ccaatcctgt tattgaacaa atcaagacaa agaccgttat tgcagatacc attgataact
    52981 ggcttgtgtc gacaaaaggg cgtgataaga atggtcaact gttagaaatt ctcaaaacta
    53041 tgcagcctta tatggctatg ctttttgtca ataccaagga acgcgcagat gatttgcatg
    53101 cctttttaac agctaatggc ttaaaagtgg ctaagattca tgggggcatt cctccaagag
    53161 aacgtaagcg catcatgaac caagtcaaaa aactggactt tgaatacatt gttgccacag
    53221 acttagcagc gcgtgggatt gacattgaag gggtcagcca tgtgatcaat gacgccatcc
    53281 cacaagactt gtctttcttt gtccatcgtg taggccgaac aggtcgaaat ggtatggcag
    53341 gaactgccat tacactttac cagccaagtg acgactcaga tatcaaagag ctggaaaaaa
    53401 tggggattgc ctttacaccg aaggtgctca aaaatggaga atttcaagat acctatgacc
    53461 gtgatcgccg tcaaaaccgt gaaaaagcct atcaaaaatt agacacagaa atgattggtt
    53521 tggtcaaaaa gaaaaagaaa aaagtcaaac caggttacaa gaaaaaaatt cagtgggcgg
    53581 ttgatgaaaa acgtcgcaaa gaacgtcgtg ctgaaaatcg tgctaagggc cgcgctgagc
    53641 gtaaggccaa aaaacaacat ttttaagaac aaagagcaag caagtgatca attggcatct
    53701 aatcttgctt tttttacacc tgctacttgt attgtttacc atttgttaac acaaaaagtg
    53761 atcatagctt ttgcttattg ctataatacg gatttactat tttacatgtg ggtctaagag
    53821 tgttagaata ttaagacaaa caaatacaaa ggggaaatac atgacatcag tgtttttaac
    53881 cagtggttgg gctttttatg attacctcat ctcaccgatt ccacatggga aactttttag
    53941 ttggcatgct gtttttgatg ccattccaaa tattatccaa cggcttccaa ttacgcttgg
    54001 tttgacacta tcaggagcaa cctttggctt ggttttggct ttgatttttg cccttgtcaa
    54061 aattaataaa gtgaagctac tataccctat tcaagctatt tttgtgagtt tcttgcgagg
    54121 aactcctatt ttggtacagt tgatgttgac ctactacggt attcccctct ttctaaaatg
    54181 tctcaatcag aagtatggct ttgattggaa tgttaatgca attccggctt ctatttttgc
    54241 cattacagcc tttgctttta atgaagcagc ctacgctagt gaaactattc gggcagccat
    54301 tttatcagta gatacaggtg aaattgaagc agcaaaaagt ctaggcatga cttctgtaca
    54361 ggtttaccgt cgtgtcatta ttcctaatgc aactgttgtg gctattccaa ccttgattaa
    54421 tgggttaatt ggcttaacta aaggaacatc gcttgccttt aatgcaggga ttgtcgaaat
    54481 gtttgcccag gctcaaattc taggcggatc agattaccgt tattttgaac gttatatctc
    54541 tgttgctctt gtctattggt ctatcagtat tttgatggag caagtaggtc gcttgattga
    54601 aaacaagatg gccatcaaag caccagaaca agctagaaat gaaaagttag gagaattgcg
    54661 ttgatgatta caattagaaa tttgagcaag acattctcag ggcaaaaagt tttagatagt
    54721 ttggctcttg atattgaaaa aggtcaagtg attgctttag ttggtgcttc aggagcaggg
    54781 aaatcgactt tccttcgtag cctaaactat ttggaaaaac cagattcagg ctccattagc
    54841 attggtgact ttacggttga ttttgagacc attaccacag agcaagtcct tattttacgt
    54901 cggaaacttg ccatggtttt tcaacaattt aatctttttg agcgtcgtac agcccttgag
    54961 aatgtcaaag aaggtcttaa ggtggtcaaa aaactgtctg accaagaagc gactaaactg
    55021 gcgcaagcag aattggctaa ggttggttta gcagatcgaa aacatcatta cccacgtcat
    55081 ttatcagggg gacaaaaaca acgggtagct ttagcaagag ctctggctat gaaaccagat
    55141 gttttattac tagatgagcc gacctcggct cttgacccgg aattagttgg cgaagtggaa
    55201 aagtctatta ctgatgctgc taaatccgga cagaccatgg ttttagtgag tcacgacatg
    55261 aattttgttt accaagtggc agatagagta ctctttttag atcaagggaa aatcttggaa
    55321 caaggaacac ctgaagaagt ctttagacat cctcaaaaag aacgaaccaa agaatttttt
    55381 gcaagctatt caaaaacata catttaatgt tattcttttg ttataataaa aaggtctagg
    55441 tatccctggg cttttcttgt tatgtttttt agaaaaactt atgggaaaga aataatgact
    55501 aggtggaaat atgttagtag aaatctttag cctttatgta aaaggtctca ttctctcaag
    55561 ccttactatg attttaattt gtttaatatg gactttatgg cgagcaaaaa ccaaaagaga
    55621 taaaacattg attgaaagac aagcttttct ttatgagatg ctaatggttg ctattttaac
    55681 catcccaatt ttatcgtttg cttttatgag tattttggtt gtcataaaat cctaatcatt
    55741 gcaagaaggg attatcattg ctaaaatatt ggcaagagta gattaaagga gaaaaatcat
    55801 gtacgataca ttaattattg gttcaggacc agcaggaatg actgccgctt tatacgctgc
    55861 tagaagtaat ttatctgttg ccattattga acaaggggca cctggtggtc agatgaacaa
    55921 cacttttgat atagagaatt acccgggata tgatcatata tctggaccag aattagctat
    55981 gaaaatgtac gaacctttag agaagtttaa tgttgaaaac atttatggta ttgttcaaaa
    56041 aatagaaaat tttggtgact ataagtgtgt gttgactgaa gatgctagtt atgaagctaa
    56101 aacggttatt attgcaacgg gagctaaata ccgtgtacta ggtgttccag gtgaagaata
    56161 ctatactagt cgaggagttt cttattgtgc agtatgtgat ggtgcgtttt tccgtgacca
    56221 ggatcttttg gtcgttggtg gtggtgactc agcagttgaa gaagctattt atcttactca
    56281 gtttgctaaa aaggtaaccg ttgttcatcg ccgtgatcaa ctaagagctc agaaaatctt
    56341 gcaggaccgt gccttcgcta atgacaaagt tgactttatc tgggattcag ttgtcaaaga
    56401 aatcaaaggt aacgacatta aggtctccaa tgtcctaatc gaaaatgtga aaacaggcca
    56461 agtcactgac cacgcttttg gaggtgtgtt tatctatgtt ggtatgaatc ctgttacagg
    56521 tatggtaaag aatctagaaa ttaccgattc agaaggttgg attatcacag atgatcatat
    56581 gaggacaagt attcctggta tttttgcgat tggagatgtt cgtcaaaaag accttcgcca
    56641 aatcacgacg gctgttggag atggtgcaat tgcagggcaa ggtgtttatc actaccttga
    56701 aagtttccct tcataaggtt aaaactcctt catttttcct tgtctttccc ttaaaaaagc
    56761 taaattgcat aagaccttta agaaatataa aggacagttt tccttaatat ctttcctaga
    56821 taaatgttga aaattttctg aaaaaaggat ataataaata gtattttagt gaatggagtt
    56881 tagaatgtct ataaaagaac aaacagacaa taacgaacta gaaaatggaa tggtaagggg
    56941 cttagaaaat cgtcatgtgc agttaattgc gattgcagga acgattggaa caggtctctt
    57001 tttaggggca ggacgctcta ttgccttaac aggaccatct attatttttg tgtatatgat
    57061 tacaggtgcc ttcatgttta tgatgatgcg tgccatcggt gaaatgctct attatgatcc
    57121 agatcaacac acctttatca attttatttc caaatacatt ggtccaggct ggggttattt
    57181 ttcaggtcta tcctattgga tttcccttat ttttattgga atggcagaaa tcacagcagt
    57241 aggtgcctat gtgcaatttt ggttcccaag ctggccagcc tggttgattc agttggtttt
    57301 cttagtctta cttagttcga tcaacttaat tgctgtgcgc gtttttgggg aaacagagtt
    57361 ttggtttgcc atgattaaga ttttagctat tttagctttg attgcaacag ctattttcat
    57421 ggtattgaca ggttttgaaa ctcacacagg ccatgctagc ctttccaata tttttgacca
    57481 tttttccatg ttcccaaatg ggaaactaaa gttctttatg gccttccaaa tggttttctt
    57541 tgcttatcaa gctatcgaat ttgtggggat taccacttct gagacggcta acccaagaaa
    57601 agttttacca aaggctattc aagaaattcc aacccgtatt gtgatctttt atgtgggagc
    57661 cttggtctct attatggcaa ttgtgccatg gcatcagtta ccagttgatg aatctccttt
    57721 tgtgatggtg ttcaaattga ttggtattaa atgggcagca gccttgatta actttgtggt
    57781 cttgacatca gcagcgtcag cgcttaactc aaccctttat tcaaccggtc gtcatctcta
    57841 tcagattgct aatgagactc caaacgcttt gaccaatcgc ttaaagatta atactttatc
    57901 tcgacaaggg gtgccaagtc gtgccattat tgcttcagcg gttgtggttg gtatctcagc
    57961 tttgattaat atcctaccag gagttgcaga tgcgttctca ctcattacgg cctcctcatc
    58021 aggtgtatat attgctatct acgccttaac catgattgct cactggaagt accgtcaatc
    58081 taaggacttt atggcagatg gttatttgat gccaaaatat aaagtgacaa cgcctttgac
    58141 ccttgctttc tttgcttttg tctttatctc tcttttctta caagaatcca cctatatcgg
    58201 cgccattgga gcaacgattt ggattattgt ttttgggatt tatagcaatg tcaaatttaa
    58261 ataataatta gcgatataga actgaatcca ttaactcttt cttatttgag gatgtcatga
    58321 tggatcagtt ctttttttac tttagcgata actcaagtta tttctaaatc ctagccaaag
    58381 agtccttttt aaaatatgtt ataatagtaa caataattca aaggagtccc ctttatgtat
    58441 aaagatgata gtttaactct ccatactgat ttgtatcaga ttaatatgat gcaagtttat
    58501 tttgagcagg gcattcataa tcgtcatgcg gtgtttgaag tctatttccg caaggaacct
    58561 tttaacaatg gttatgctgt ttttgccggt ttacagcgca tggtggagta cctagagcaa
    58621 tttcaatttt cagaaacaga tttggcttac cttgaagaac tgggttatcc agaaaacttt
    58681 ttgacctacc ttaaagagtt acgattagaa ttgaccatcc gttcagctaa agaaggagat
    58741 ttggtttttg ctaatgagcc gattgtgcaa gtagaaggcc ctctaggtca gtgtcagttg
    58801 gtcgaaacag ctcttttaaa cattgttaac ttccagacct tgattgctac aaaggctgct
    58861 cgtattcgtt ctgtgattga ggatgaaccc cttttggaat ttggcacccg tcgtgcacag
    58921 gaactagatg ctgcgatttg gggaacccgc gcagcgatga ttggtggagc agatgcaaca
    58981 agtaatgtga gagcaggtaa acgctttgat atacctgttt caggtacgca tgctcatgct
    59041 ttagtgcaag cctatggtaa tgattatgat gctttcatgg cttatgctaa gacccataaa
    59101 gattgtgtat ttcttgttga cacctatgat acacttaaag taggtgtgcc aacagctatc
    59161 cgagtagcca aagaaatggg tgacaagatt aattttctag gtgttcgatt ggactctggt
    59221 gacttggctt acctctcaaa aacagttcgt cagcagttag acgacgctgg ttttaccgag
    59281 gctaagatct atgcttctaa tgacctcgat gaaaatacca tcctcaactt aaaaatgcaa
    59341 aaggccaaaa ttgatgtttg gggtgttggg accaaactga tcacagccta tgatcagcct
    59401 gccttaggcg ctgtttataa gattgtctct attgagcaag aagatggtag catgcgtgat
    59461 actatcaaat tatcaaataa tgcagaaaaa gtatccacac caggtaaaaa acaagtttgg
    59521 cgcatcacca gtcgtgaaaa aggcaaatca gaaggcgact atatcacgtt tactgatatt
    59581 aatgttaatg aattaactga gattgagatg ttccatccga cctatacgta tattaagaaa
    59641 accgttaaag aatttgatgc aattcctctc ttagttgata tttttgtcaa gggagaatta
    59701 gtttaccagc taccgacctt agctgaaatc aaggcttatg ctaaaaaaga atttgacaag
    59761 ctttgggatg aatacaagcg tgtcttaaat cctcaagact acccagtaga cttggcacga
    59821 gatgtgtggc aaaataagat gaccttgatt gataacattc gtaaagatac tcatggaaag
    59881 agtgaatgac atgacattac aagaagagat aattcgacaa ttaggcgtca aggctagcat
    59941 tgatccacaa gaagaaattc gaaaaaccgt tgatttttta aaagcttatt tgcgtaagca
    60001 ctcattttta aaaacttatg tcttaggcat ttcgggaggt caggattcta ccctcgcagg
    60061 aaagctagcc cagatggcca ttgcagagtt acgggaggaa gcaagtgatc aagcttacca
    60121 atttatagct gttcgcttgc cttatggggt tcaggctgat gaagcagatg cgcaaaaagc
    60181 cttggctttt attgcaccag atcagaccct taccattaat atcaaggcag ctgtggacgg
    60241 tcaagtagag gctctacaag cagcaggcgt tgagatttct gatttcaata aaggtaatat
    60301 taaagcacgt cagcgaatga tcagtcaata tgccattgct ggccaaatgg ctggtgctgt
    60361 tattggaacg gatcatgcgg cagaaaacat cactggtttc tttactaaat ttggtgatgg
    60421 tggagcagat attcttcccc tctttaggct taacaaacgt caaggaaagg ctcttttgaa
    60481 agttttagga gcagatgctg ctttgtatga aaaagttcca actgctgatt tagaagatca
    60541 aaaaccaggc ttggctgatg aggttgccct aggggtgacc tatcaagaca tcgacgacta
    60601 tttggaaggg aagctgattt ccaaagtagc tcaagcaact attgaaaaat ggtggcataa
    60661 aggacaacac aagcgtcacc tgccaatcac catttttgat gatttttgga aataagagct
    60721 gatgttcaaa gagtaaaaaa aggaaacaaa aaaagttagg ttatcctaac ttttttcttg
    60781 aagaaaatca aataaggtct ataatagaag taatccttta gaatagaatg gctggataat
    60841 aaccatcttg ccaatattat ttcaggtgaa gcttatctta atattttagg aggacaataa
    60901 tgtcagcatt aacagagaca tttacagaac aattatttgc tcattacgaa gccaatgcaa
    60961 aattttcagc tattgagaat gccgttacgc ataatggttt gctaaaatca ttagaaaccc
    61021 gtcaaagtga agtggacaat gattttgtgt tttctattga tttaactaaa gataaggtat
    61081 ctaaccaaaa ggcttctggt cgttgctgga tgtttgctgc cttgaacact ttccgacaca
    61141 aattaatcac agaatttaag ttagaaaatt ttgaactgtc acaagctcat actttctttt
    61201 gggataagta tgaaaaagct aactggttta tggagcaggt cattgctaca gctgaccaag
    61261 aattaaccag tcgtaaagtt aagtttctct tggatgttcc acaacaagat ggtggtcagt
    61321 gggacatggt ggtttccttg tttgagaagt atggagtggt gccaaaatct gtttacccag
    61381 aatcaatctc atcaagtaac agtcgtgaat taaaccagta tttgaataaa ttattgcgcc
    61441 aagatgctca aattcttcgt gacctgattg cgtcaggtgc taaggcagac caagttgagg
    61501 atcgaaaagc agagcttctt caggaaattt ttaacttctt ggccatgacc cttggtttac
    61561 cacctcgtca ttttgatttt gcctaccgtg ataaagatga ccactatcat gttgagaaag
    61621 gtttgacacc gcaagcgttt tacgacaaat ttgttggctt aaagctttct gattatgtgt
    61681 ctgttattaa tgcgccaact gccgataagc cttatggcaa atcatacact gttgagatgc
    61741 taggaaatgt agtcggtagt cgtgaggttc gctacctcaa ccttgatatg aaacgtttca
    61801 aagaactagc tatcaagcag atgcaggctg gtgagtccgt ttggtttggc tctgatgtgg
    61861 gtcaagtttc agatcgtcaa aaaggcattt tggcaactaa tacttacgat tttgaagcta
    61921 gtatggatat caatcttagt caagataagg ctggacgttt ggattatagt gaaagtttga
    61981 tgacacatgc tatggtttta acaggtgttg acttagatga aacaggaaaa ccgttaaaat
    62041 ggaaggttga aaattcatgg ggtgagaagg ttggtgacaa aggttacttt gttgcttcag
    62101 atgcttggat ggatgaatat acctatcaaa ttgttgtccg taaagaattc ttaacagctg
    62161 acgagttagc ggcttatgaa aaagagccac aagtactagc tccttgggat ccaatgggtg
    62221 ctttagcaca atactaattc ctttctttgc gactgtatga aatctaaaag ctggacaaaa
    62281 cgaacagcaa acggattgga ctccgcataa gaaagtgaac atgaaaaaaa ttattatgct
    62341 aagaaaatga catatgaaaa aacacgtctt cggtatatgg aaacgtgttt ttttcatgta
    62401 tactctctta tggcattagt tgctatttat aacaatttta aataagtcta ttttgtttta
    62461 ggttaacgtt tgaaaaatca gttgctaatt cccttaacgt tgtacctttt ctttaatacg
    62521 attaaagaat tctgtttttt gttttatatt gtatgtggta agtcatttgt gtcatataaa
    62581 aagcgagtcc tcacggaacg tggactcgct ttttatttag attatcactt aaggtagata
    62641 ttaaaagtaa ctaaatggat atgtaataaa aggtaggtac agacaataag tagaggttag
    62701 agattaatga gttgtctttt tatcatcggt agatggatga ctgccattgt tgtctgtatt
    62761 ggttgaatta cttgtatcat tgctactact agtatcatct gaagtcgttt gactagaagc
    62821 tgttgtcgtg ttattgctgt aaagattgtt gtaaaccgaa ttagtatagt cggtgttgct
    62881 cgcataggtt ccgcttaagt agaggaatcc accactgcga taaagaccat ttggcatcgt
    62941 ccaatcttca ctgtaaccat ttgttaagta agtcatcatg ctacgataaa cgtcagatgc
    63001 aatctctaga cttgatccgt atactggggt caagcggttt ttgtaacctg tccaaacagc
    63061 catggcgtag cgcttagtaa agccaacaaa gttttcgtct ggcgctaatg taccaacata
    63121 atctggataa aggccgtatt tttcaccaat tttagctagt tcctcatcag tgtagttaga
    63181 agtccctgtt ttaccagctt gcgcaacacc aggaatggca gcagcagtac ctgtaccata
    63241 agtgagaaca gtttttaaca tatctgtcat catataggcc gtggtttctt tcatggcacg
    63301 ttttcctttt tcatcaaaag ttttacttgt accatcacta aattccactt tattgacata
    63361 ccttggttta tgataaatac caccatttgc aaaagcagcg tatgcagcgg ccattttttc
    63421 actacttgca ccatattttt tatctgagct actgttatta cttgagatag cgtttgagta
    63481 gtgcatttcg ggatagttaa tacctaaact gcttaagaaa gatcgagcat agtcaagtcc
    63541 tgcggcttcc agtgctcgga ctgctgggac atttcgcgat agcataatag cagcttggat
    63601 tgtcatccat ccgttatatc taaggtccca gttaaacaat tgggtagtgg ttccaggcca
    63661 ataatagact gagtcattag tcgactgagc agtagaagta taaacaccag attcaatagc
    63721 aggagcatag gctgtgattg gcttcatggt agaaccccag tcacgatcag ttaaaacagc
    63781 ttggttagtc ccaaatgaaa cattttcatc ttgattacgt ccgccaagct gagcaataac
    63841 atgaccattt gtcacatcaa caatcgttga agcaacttgg aaatcttggt ctggatagta
    63901 aacataatca ccagaatgat aaatattata aagagtctgc tgcgcatcgg ggataatatt
    63961 ggtataaact tttaaaccag cggtaaaaat atctttattt gtttcttttt tgacttcttc
    64021 aataacttgt tttagataat tatccatata ttttggataa gttgagcgct gttggagtga
    64081 ttgtagacct tcagcgacgg gagttgcgat ggcagtttca tattctgctt tcgtcagatg
    64141 tttttccatg tacatctgtt gcaacacgac gttacggcga ttttgagcag cttcaggatg
    64201 aaggtaagga tcatattgac taggagcttg agggattcca gccaataggg ctagctgggc
    64261 ataagataaa tccttaagat ccttgccata ataagactta gcggctgtca gcataccata
    64321 gttgccatta cccatatata ctttgttgat gtaaaaagtc aggatttctt gtttagtata
    64381 ttttcgctcc atttgaagag caagccaaac ttcttgagcc ttacgtttta aggtttgatc
    64441 agattcatta gtagaaaaat aggctagttt aatgagttgc tgatcaagcg ttgacccccc
    64501 ttgagtggtc tgactcgtta gattatgaaa ggcagcacca aaaatacgat aaagatctac
    64561 tccacgatgg ttaaagaaac gtttatcttc aattgaggta atagcattaa ctagattaat
    64621 agggatacta tcagctgtta cattttcacg cttttcagaa cccaaatcag caatcagatt
    64681 gttattaccg tcataaacca agctagagtt tgttgatttt aactgggctt ctgacagttt
    64741 cggagcactg ctaatgtaga aggtaaacaa aagaccacca ataataataa caaggataat
    64801 aaggctaaga attgcactta atacatactt tagccactta aggatttttg gatttttaat
    64861 tgtaatcacc gcctaaaaaa ttttgttcaa ttatgtttaa ataagggact tgtggaaagg
    64921 ctccaaatgc taccttaaag ccattttttc tgatataatc aatgggcatc gatttactgc
    64981 cattatctat ctgataaaat gaaatcaagg cttgagcagg taggtagtaa gtctccttca
    65041 gtgtggaaaa gtgaagaagg acaaagcaga ttcctttttg atgtaaaacg caagccatat
    65101 gttcaatctg atgtaaatgg aaatttttca tgggcatagc tgttttttgc ctggtttctt
    65161 ttgcttcaaa atcaacataa tgtcctttat aaacgccgca atagtcagtc gttgaagctt
    65221 gtctaaagta cgcttcaacg atttttgccc gactgcgttt agggtaatcc actttgacaa
    65281 tttggacagg agtaggtttt ttgtgaatga cagctatttg acgagataaa taataatcat
    65341 ttgtcgcgtt gatagcagct tcaaaggaca tacctctatt ggcgaagtcc accttatttt
    65401 gctttgtttg cttttgaacg gaacttactt tttgtcgaat aagattatga ggataattaa
    65461 ccattgttct cctaatatta tctagttaat gctaaaaatt taagcacttt tattataaca
    65521 tattttttga aaggataaca cctaatgact gcgattctaa ttacaggtta ccgaagcttt
    65581 gaaataggga tttttgacca taaagaccca cgagtttcaa ttattaaaca ggctattcgt
    65641 aaagatttaa taagttattt ggaaaatggt gtggattggt ttatctttac agggaacttg
    65701 ggttttgaac aatgggcttt ggaagtagct aatgagctaa aagaggagta tcccttgcag
    65761 atagctacta tattcttgtt tgaaacgcat ggagataggt ggaatgaaaa aaatcaagaa
    65821 gttttaagtc agtttagggc tgttgacttt gtaaaatatt actttccaaa ttacgaacaa
    65881 ccgatgcaat ttagtcaata ttatcagttt ttattagaaa agacagaagg ggcttatgtt
    65941 ttttatgaca cagaaaacga gaccaactta aaatattttc tcaagaaagc aaaagatatg
    66001 ccgcattatc agttgctatt gctaaccttt gatcggctaa atgatatgtc acaaagctag
    66061 cattgtattt ttaggggtat tggtatttaa atacttgctt tttgaactat ttttctgtat
    66121 cattaataat aatactgaaa tataattttg gtaaaaagat tgaccgttcg gagagtaaag
    66181 aatgacaagt attatttata gtcctaaaga tatttttgag caagaattta agactagtat
    66241 gcgagggttt gataagaaag aagttgatga atttcttgat aatgtaatta aagattatga
    66301 aaatttcaat gctcaaattg aagctttaaa agctgagaac gaagccttaa aaaaggccaa
    66361 gtttcaagct agaaatacag tttcagcgac tgttcaacaa ccagtaccgc aaccgactcg
    66421 tgttgctcaa tcagctacta attttgacat cttgaaacgt attagtaaat tggaaaaaga
    66481 ggtttttggt aaacaaatta ttgagtaatg aaaactgtgc aatttttgga taatcgcgta
    66541 gtattttaat actatgagga aagtccatgc tagcactggc tgtgatgcca gtagtgattg
    66601 tgctaggcga acacataagc ctagggatgt gcatacacat tacggcgaag gaaatggcta
    66661 agtcgtttga tatgccaaag tacttctgaa agtgccacag tgacgtagtt tttatggaaa
    66721 cgtaaaaaat ggaacgcggt aaacccctca agctagcaac ccaaactttg gtaggggcat
    66781 gggatagttg gaaacgaaca agctatcctg actgtcaaca gacggtagac agatgattat
    66841 cgaaggaaat aattcctagt tatttccgga acaaaacatg gcttatagaa aattgcataa
    66901 taggttaagc tagcacttgc tagcttttat ttgatttaaa agaaagagat atatgaaaga
    66961 aacatttaga ttagtcgcaa ctgcagccgc aggtttagaa gctgtcgttg gtaaagaagt
    67021 cagagcacta ggctttgatt gtcaggttga aaatgggaaa gtttattttg aaggtgatgt
    67081 agaagcgatt gttaaaacaa atctttggtt acgtgcagca gatcgtatca aaattatcgt
    67141 aggacaattt cctgctagaa cctttgaaga attattccaa ggtgtctttg ccttggattg
    67201 ggaaaattat ttaccgctag gagctaaatt tccaatctca aaagccaaat gtgttaagtc
    67261 taaattgcat aacgagccaa gtgttcaggc gattaccaaa aaggccgttg ttaagaaact
    67321 gcaaaagcat ttccacaggc cggaaggagt tcctcttcaa gaagttgggt caacgtttaa
    67381 cattgaggta tctattttaa aagaccaagc cactattatg attgacacga caggatctag
    67441 cctatttaaa cgagggtatc gcgttcaaaa aggtggagca ccgatcaagg aaaatatggc
    67501 agctgctatc ttggccttat ctaactggtt tccagataaa cctctggttg atcccacctg
    67561 tggatcggga acgttttgca tcgaggcagc cctgattggg atgaatattg ctccaggatt
    67621 taaccgttcg tttgcttttg aagaatggag ctgggtagat aaagacatgg tgcaacaagt
    67681 tcgtgatgat gctgaacaag aagctaatta tgaaatcgaa ttagatattt ctggctttga
    67741 tatcgatggc cgcatgattg agattgctaa aagtaatgct gaagaagctg gtttatctga
    67801 tgtgattacc tttaagcaaa tgcgtctgca agactttcga acagacaaga ttaatggtgt
    67861 agtgatatca aatcctcctt atggagaacg gttgcttgat gacaaagcgg ttgacatctt
    67921 gtataatgaa atgggacaaa cctttgcacc attgaaaaca tggagcaaat ttatccttac
    67981 aagtgatgaa ctttttgaac taaaatacgg gcaaaaagct gataaaaaac gtaaacttta
    68041 taacggcacc ctaaaagttg atttgtacca attttatggg gaaagggtaa agcgacatct
    68101 gatatagaga ggtagtaagc gtgtcagaag aaagcaaaga agtcgaagta acaaaagaat
    68161 cgcaaacatt ggggcttaat gaagccaagt caatgacaat tggtgaagct gtacgcaaac
    68221 agtcagagat aaaagcaggt gttactaagg atgatagtat tttagataag tacattaagc
    68281 aacaccgtga cgaagtttct tctcaaaagt ttgatgctaa gtatacagag cttgatacag
    68341 ctagtttaga taattttatc aaaaaacaaa gagaagcatt gagtaaagcc ggtcttgttg
    68401 atgacgaacc tgtgtctgcg gagtctgctg agcaagatag cactttggtt gaagaagtag
    68461 ctgaagactt agctccaatg gagactactg cggtggtaac tgggattccg gtggaagcga
    68521 cagtccctgt gttagatcta gatccatcag agagggtcat tccagaacca cagatgacca
    68581 aggaagaacc aaaaagagat cagtttcttt ctgaagatag ccaccatcca gccaaacaaa
    68641 acacgaaaaa aggttggtta atagctttat ttctgctatt attggccatt cttgcagttg
    68701 tatttggttg gaaccatttc ttgagacaag acagtggtaa aacaactcaa acagcaagta
    68761 aacagacaaa gacctctttg cagaccgaca gtgctaaaaa agcaacgcgc ttgaaggctg
    68821 ctgctaaagc tttcgaaaaa caatatggta ctttttatac agatgccaca aagagcaaat
    68881 taaaaaatag tgcctttgct actttgccag atttagaagc tgccttgaaa gctttggaaa
    68941 gctctgctta ctatgataag gcgaaagcaa aagtcgattc ccttaagaaa gctattgctg
    69001 ctattacagc ggttaacggt aaatttgttt ccgatgctgt tgtagatggt gaaaaggttt
    69061 cggctgaagt caaggctgat gctaattttg atgatttatc aagtgcaacc ttgacaacag
    69121 gaaatgcaaa tcttgatgct gtcttgcagg caagcatcac agaaggtcgt cagcaactag
    69181 ccagcaaggc tgaggccgct aaagcagcta atgagcaagc agcccaggct caagcagctc
    69241 aaggacaaag tacatcagta gcaccatctg gctatggctt gacaagttat gaccctgcaa
    69301 gcttacaacg gcatttatca cgtgtacctt acaatcaaga tgttattgct gacagagcta
    69361 acccatcttg ggcatttaat ccaggagttc ttgaaaaaat tgtagcaacc tcacaagcta
    69421 gaggttatat ttcaggcaac caatatattt tagagccggt taatattatt aatggcaatg
    69481 gttactataa tatgtttaag cctgatggca cttatctgtt ctctattaat tgtaagacgg
    69541 gttattttgt aggaaatggc aaaggttatg ctgatgcttt agattactga gaaaatcaaa
    69601 aaaactgaga taaggcaact tgtctcagtt tttttgatta ataaagtatg ttgtcttaaa
    69661 atctgcagat agacttgttt tttgtaacaa gtcaaatagg tcagtaggat tgttatgttt
    69721 gacaacacac tagtatcaga tgacatggcg actaaaagga tcgtctgaaa tcccttgatc
    69781 aataatcaat tgagcccatt ctttggcggc aaagaggcta tgatccttat agttcccaca
    69841 ggattcaaga gttgttccag gaacatcttc ccaagtaatc ccagttgcaa tttcttctag
    69901 gctggattta atcaccttgg caatatcagt agaactgtgt tttccccaca tgataaggtg
    69961 aaaacctgtt cgacagccaa aaggagagca atcaatcatc ccatcaatgc gttggcggat
    70021 gagcttggca agtaagtgtt caatggtatg caaaccggct gtttcaatag aattttggtt
    70081 aggctgcact aggcgaacat caaaatttgt aatacgatcg cccttgggtc caaattcttc
    70141 agaaataaga cgaacataag gggcctttac aatagtatga tctagctcaa aactttcgac
    70201 gataacttct tttgtcattt caatctcctt tgttttacac tttatcataa cacaaatagt
    70261 gagaaagtgg ctacttgtgg aggagaaaag gctaagaaaa cagatcaaag gctatcataa
    70321 tgacctgtgc gataaaagta cttacagaaa cagtaataaa ggtttcttta aaagcatttt
    70381 tgtggttaag tcctgtcaat gctaagaagg tgagaaagac accactttgg ggcacaatgg
    70441 taaagatatt agaagcaata gtggcaacac ggtgaatcat ttcgggatta aggccttgat
    70501 ctaaataata ctgggcaaag ttaggcatga caattcctag agcaccggaa gaagagccgg
    70561 taatagctga catagaggaa gtcaacacgg ctaaactaat aagaggatta ccgggaatat
    70621 tcaaaatgag atctgagaag aatgtgaaac ctggggcaat catcacaacg gctccgaagg
    70681 caactgcaga tgctgtcgca aaaataggtg caatagagct gctagctcct aaattaaaga
    70741 cagcaatctt attaggaata aactgtctaa agagccagct tgcggtgaga atggcagcta
    70801 aaagtgctat gaaaataatg ttcttcttaa aaaaatcgtt tccgaaaagg ctaccggtta
    70861 gggcgataat aatgagcaac aataaaggta agatactggc aaggaaatgt gggagatttt
    70921 ttgttttcac ttgtatctca ttgtcaaaag cataagtggc ataagtttca ccccttgcca
    70981 tactttttgc tagacaatat ttcatataaa agagcccaaa agtcacgcag ccaattgagc
    71041 caacgatact cggaatggcg gcagcagtta gtgaggtgtc caagtattga ataggaataa
    71101 cattttgaat ggcaggtgtt ccgggtaaaa tcgtcatggt aaaagttgca ataccgagcc
    71161 aaagaggaac ttgaattaag ttccaagcca agtccatttt tttgaataga cttcgtgcaa
    71221 gcggcaagac agcaaacatg acaacaaaga ggctgattcc cccgtaagtt aatatagcac
    71281 tgattagaaa aatagcaacg agtactttat aaggactatc atgaccaact ttttttaaaa
    71341 tataatctgc aatagaagtg gttgcaccgc ttgtttccat taatttggct aagatagaac
    71401 ctaataaaaa gatagcaaaa taattgagaa tataagtgga gagtgccccc ataaattggt
    71461 tgggttcttt tccaagtaag gttgaggttg gatccatttg attaaacaaa atgaccaagc
    71521 tggtagcaag aggcgcagcg atgataatgt taacttcttt gacagacagg taaataataa
    71581 caataacacc tatcaagacc cccagagaac caataatttc catagtttta tgacctcctt
    71641 attagaaaat aaagagacag atcacaagat acagcctctt tatctctcca aatttattga
    71701 gcagtgtagc caccgtctaa gatacaggct tgacctgtaa cgccttttgc cttatcactg
    71761 gcaaggaaag acacatagtc tgcaatttct tgaacgtcaa tgaggcgttt ttgaggaaca
    71821 agtgggtata gcacttcttc aagaacattt tcaaggggaa tacctcttgt tttggaaagg
    71881 tcttcaaact ggccacgaac cagtggtgtg tcaacatatc caggacaaat ggcattgacc
    71941 gtaatgcctg aatcagcagc ttctaaggca gttactttgg tcagaccgat caagccgtgc
    72001 ttagctgaat tgtaggcgga tttgccagca aaaccaatga caccattgat agaagccata
    72061 ttaataatac gaccgtgttt ttgagctttc attgtaggaa aagcacgttt aatggcaata
    72121 aatggtgctg tcaacatgat tttaatcatg aattcaaatt tttcagttgg aaaatcttca
    72181 atcatggcaa catgttgaag gcctgcgttg ttaaccaaaa tatcaatacg accatatttt
    72241 tcaacaaccg tatcaatagc agcattgatg gcttcttctt tggtgacatc gcacacaaca
    72301 ctaaaagcgt catagccttc tttagtatag tcagcaacaa cctgatccag tttttcttga
    72361 ttaatgtcag aaaaaacaac cgttttacct tcttttaaga aggtttcccc gatttgtttt
    72421 ccaatcccgc ttgcagcacc agtgataaat gcaacttctt tagtatgtgt catgatacta
    72481 tcctcgtctt tcttttggtt agtctttagt ttgttttatc aattagggta acttcggtaa
    72541 cgttttgcac gtcttcaaag gtgtaatctg ggttaatttc aagggcgcat agaccttcat
    72601 cttgatattc aaagactccc atttcagtaa tgattaggtt aacaacattt tgagcagtga
    72661 gaggaagggt gcatttgtct aaaatttttg ctttgccttt attggtatgc tccattgcaa
    72721 caatgacttt tttagcacct accaataagt ccatggcccc tcccatccca ggaaccattt
    72781 ttccaggaat caggtaatta gcaatgctag cttctttatc aacttgtaag gcacctaaga
    72841 cggttgcggc cacatggccg cctcgaataa taccaaagga agttgagcta tcgaagaaag
    72901 cccctccagg agcaatacta acaggttgcc caccagcatt aacaatacta ggatcgtagt
    72961 gatcatctgt taatccagtg agaccgacaa aaccattttc ggactgcagg gtaatggtta
    73021 caccttcagg aatatagttg gctacttttg ttggcaaacc tatgcctaag ttgaccagtg
    73081 tattgtgttc taattcttgt gcaacgcgct tagcaatacg tgtttgaatc tcttcttttg
    73141 aaagaacagt agctgatgtt cccatgttag gctcctttca ctaagtagtt aacaaaaatg
    73201 ccaggagtat ggacaaaatt aggatccatt tgccccacgt caacaatttc tcttgcttca
    73261 acaatggtgg ttttcgcatt agcagccatc acatgattaa agttattttc agaacctgcg
    73321 tattgcaaat tgccattttt atctgccttg ttagcaaaaa taagtgcgat atcagctttc
    73381 aaaggttttt caagaaggta gtctttgccg tcaatagtaa tgacttcttt tcctttggca
    73441 acttcggttc ctattccagt aggtgttaag aaaccgccta gcccaaaacc gccactgcgg
    73501 atgcgctcag ctaatgttcc ctgaggtaca agatcaatta cagtctcgcc ctcagtcatt
    73561 tgtcggcccg cttcacggtt gagaccgata tgtgatgcaa taatagttga aaactgttta
    73621 ttggcaacca tcttaccaac gcctttgtca ggaaaaccgg catcgttaca gattaaagtg
    73681 aggtctttga cacctttttc cacaagtgca tcaattaatt tttctggtgt gccatttgtc
    73741 atgaagccac caaccataat ggtatcacca tctttaacgt gagctaccgc ttcttttatt
    73801 gtgatttctt tacacatttt ttctaccaac tttcacgaga ctaagtattg tttttcacga
    73861 taacagcagt tccttgtcct ccaccaatgc aaagtgttgc tagtcctctg gttacttggc
    73921 gttttttcat ttcgtggatt aatgtgacta gaatgcgagc gccactggca ccaataggat
    73981 gacctaaagc gatggctcct ccattaacat tcacgatttc tgggtttaac tttaaatctt
    74041 ttaaaaccgc caaggactgt gctgcaaatg cttcattgga ctccactaag tccaaatcag
    74101 aaattgttaa tccagctttc ttaagggctt tttgagtggc aggaattggt ccagttccca
    74161 tcagttcagg tgcaacacct gcactagcat agctttcaat agtgactaaa ggtgttaacc
    74221 ctaattcaag agctttttct tcagtcatta acatcaataa ggctgcacca tcgtttattc
    74281 ctgaggcatt cccagcagta acggttcctt ctttagggag gaaagcagga cgtaattgct
    74341 gtaatttttc aaggcttgta tcaacttttg ggtattcatc tgttgtaaca attaaagggt
    74401 ctttgcgtcg ttgaggcaca gagacaggga caatttcgtc agcaaaacga tgattttcta
    74461 atgctttagc agctttttct tggctttcta aagcaaattg atcttgctct tctcttgaga
    74521 tgccatattt ctgagcaacg ttttcagcag taatacccat gtggtaattg ttaaaagcat
    74581 ctgttaagcc atcatgaaca agagtatcaa tcaattgact attaccaaga cgttgaccaa
    74641 agcggtgctg agttgagaca taagctgctt gtgacatgtt ttcgacacca ccagcaataa
    74701 cgatatcagc gtcaccaagc atgatgcttt gcgcagctaa ttggattgct ttaagtccag
    74761 aaccgcaaac catatctaat gtgaaagctg ttttatcttc aggaattcct gagtggactg
    74821 caacttgtct tgccacattt tgtcctaggc ctgcatgaag gacgtttcct aggatgactt
    74881 catcaaccat ttcaggcttt acttgcgttt tatcaaggat ttttttcagt acagttgttc
    74941 caagttcaac agcagagaca cttttaaagg agcctccaaa agctccgata ggagttcgta
    75001 aagcttcaac gataactatg tttttcataa aataaacatc ctttcagttt tagaaagcgc
    75061 ttttcatgtc tctaagataa caagaaaacg ctaaaaataa aaatatagaa tttttataag
    75121 catataattt tttttgatag agttggcaat agcatttaac atctacttgt gtcataatat
    75181 aggtaattat accggaaaaa gtaattgata agacaaggag aatcctattt ggatattaaa
    75241 cagattcgtt attttatcgc aatcgttgaa aatcatttta acttgagcca ggcggcggaa
    75301 ctgttgtatg tttctcaacc gacgctgagt atgatgatta acgattttga aaaaagagaa
    75361 aatgttaagc tcttcaaacg aaaaagaggt cgtattattg ggttaaccta tcttggtgat
    75421 aattattata aggacgctca aaaggttctc agtctctatg atgacatgtt tttgaaattg
    75481 catgatcaca gtaaagggtt aaagggaagt atcagtattg gtattcctcc tcttatttta
    75541 tcagttgttt tctcagaagt aatgccaaaa ctgattttag aaaatccggg gattcaattc
    75601 aatgttaagg agattggtgc ttaccaacta aaaaatgaac tactcgtggg aaatgttgac
    75661 gtcgcagtct tgttatcccc aacaggcatc gctgataatt tggttgagac ttatgaaatc
    75721 cagagatcag aattatctgt atgcctgtct ccacgccatc gtttagcttc caaaaaagtt
    75781 attcagtggg aagatttaac agatgaacaa cttgccctat ttgatcctag tttcatggtt
    75841 caccatttag tgcttgaagc ttgcgagcgt caccaagtca gacctaatat tattttgacg
    75901 tcctcttcct gggattttat gcttaattct accaagatta atcacaacgt cttaactatt
    75961 tgtcccaaac ctattacaga attataccaa ttcaaggaca ttaagtgtat tccaatggaa
    76021 cgtcccattt cttggcgtgt tgtcttaaca cgcctccgga aaaaaagtta ttctgaaata
    76081 gaagcctaca tcatggacga cttgctacag tcttttttta agtagggttc tcaactactt
    76141 ggcccaaatg ttaatttttt taaaaatgaa ttggaattgt gtaaacgctc aatttatgat
    76201 aaactaagag atggctttct attaatgaaa tctagaatat attgaggtac aaacatggtt
    76261 aatattattt tattaattgt ttctgccctc attggtttaa tattaggtta tgcacttatt
    76321 tcgattagac tcaagtctgc gaaggaagct gcagagttga ctcttttaaa cgctgaacaa
    76381 gaagctgttg atattcgtgg caaagcagaa gtagatgctg aacacatcaa aaaaacagct
    76441 aaacgtgaaa gtaaagcaaa tcgtaaagaa ttacttttag aagcaaaaga agaggcaaga
    76501 aaatatcgtg aagagattga acaagaattt aagtctgaaa gacaagagct taaacaactc
    76561 gagacacgct tagcggagcg ctccttaact cttgaccgta aagatgaaaa cctatcaagt
    76621 aaagaaaagg tactagatag taaagaacaa agtctgaccg ataaatctaa acacattgat
    76681 gagcggcaac ttcaagtgga aaaacttgaa gaggagaaaa aagcagaact ggaaaaagtt
    76741 gctgcgatga cgattgcaga agcgcgtgaa gtgattttaa tggagacgga aaacaaactg
    76801 acccatgaaa ttgcgacgcg cattcgagat gccgaacgtg acatcaagga ccgaacagtt
    76861 aaaacagcca aggacttgtt agcgcaagcc atgcaacgcc ttgctggtga gtatgtgact
    76921 gaacaaacta ttaccagtgt ccatctccca gacgacaaca tgaagggccg aattattgga
    76981 cgtgaaggcc gtaatattcg tactttagag agcttgactg gcattgacgt tattattgac
    77041 gatactcctg aagttgttat cttgtcagga tttgatccta ttcgacgtga aattgctcgt
    77101 atgaccttgg aatctctgat tgctgatggt cgcatccatc cagctcgtat cgaggaattg
    77161 gttgagaaaa atcgtcttga aatggataat cgtattcgtg agtacggtga agctgcagcc
    77221 tatgagattg gtgcaccaaa ccttcatcct gatttgatta aaatcatggg acgcctgcaa
    77281 ttccgtacct cgtttggtca aaatgtccta cgtcactctg ttgaggttgg taagttagct
    77341 ggtattttag ctggtgagtt aggtgaaaat gttgctcttg cccgccgtgc tggtttcttg
    77401 catgatatgg gtaaagctat tgaccgtgag gttgaaggta gtcacgttga gattggaatg
    77461 gaatttgcac gtaaatacaa agaacatcca gttgttgtca acactattgc tagccaccac
    77521 ggagatgtgg agccagattc tgtgatcgct gtgctagtag ctgcagcaga cgctctcagt
    77581 tcggctcgcc caggcgctcg taatgagtca atggagaatt acatcaagcg tcttcgtgat
    77641 ttagaagaaa tcgcgacaag ttttgatggg gtacaaaata gttttgctct acaagctgga
    77701 cgtgagattc gtattatggt tcaacctgaa aaaatttcag atgatcaggt tgtcattttg
    77761 tcgcataaag taagagaaaa aattgaaaac aatctagatt acccaggaaa tattaaagta
    77821 actgttattc gtgagatgag agcggttgat tatgccaagt agaaaaagag gaattatcct
    77881 ctttttcttt atgacttgcc tttgaaaatg ttcttgaaaa agactagcct ttttgataaa
    77941 ctattaaaaa gcaactaata aggagaatac aatgtctgaa cgcggtttgt taatcgtctt
    78001 ttctggccct tcaggtgtcg gaaaaggaac ggtaagacaa gaaatttttt caacgccaga
    78061 tcataaattt gaatattcgg tttcaatgac gactcgtccg caaagaccgg gcgaggttga
    78121 tggggttgat tatttcttcc gcacacgtga agaatttgaa gaactcatta aaactggtca
    78181 aatgctagag tatgctgaat atgttggtaa ttactatggc acccctttaa cttatgttaa
    78241 tgagactctt gataagggaa ttgatgtctt tttagaaatt gaagttcaag gagctcttca
    78301 ggttaagtca aaagtaccag atggtgtttt tgttttttta acaccaccag atttagatga
    78361 attagaagac cgcctggttg gtcgtggtac agatagtcaa gaagtcatag ctcaacgtat
    78421 tgaaagagca aaagaagaaa ttgcccttat gcgcgaatac gattatgcag ttgttaatga
    78481 tgaagttgcc ttggcagctg agcgggttaa gcgtatcatt gagacagaac actttcgtgt
    78541 ggagcgcgtg attggtcgtt atgataaaat gattaaaata acaaaaaatc cctttaaagc
    78601 taaatagaaa agagtaagca ttatgatgtt aaaaccttcc attgatacct tattagataa
    78661 agtaccatct aagtattcac ttgtgatttt acaagccaaa cgtgcccatg aactagaagc
    78721 tggtgccact ccaactcaag aatttaaatc agtgaagtca acacttcagg cacttgaaga
    78781 aattgaatca ggaaacgttg tgattcaccc agatccttca gctaaacgtg aagctgtccg
    78841 tgctaagatt gaggcagaac gcttggccaa agaggaagag gaacgtaaaa tcaaagaaca
    78901 aattgccaaa gaaaaagaag aagaaggaga aaaaatctaa ggtttcagaa atcttagatt
    78961 ttttcatatt tagtacgatg tccatgacag aaaaggagaa gcaaatggaa aaagtagctc
    79021 atgtgattgt agatattcca ttgatgcaaa cggacaagcc attttcttat ggtattccaa
    79081 aagagttagt ttctctcgtg cagcttggtt ctcgtgtgca tgttcctttt ggaaaaggga
    79141 atcgtttgtt gcaaggcttt gttattggtt ttggccagga agattcaagc tctcttaaac
    79201 tgattcagac cgttttagat cctgagccag tcctaaacca agaacaattg acccttgctg
    79261 atcagcttcg aaaaacagtt ttttcttata aaatcacgct cttaaaagca atgattccta
    79321 atcttcttaa ctctaactat gataaagtgc taaggccaga gtcaggtcta aaaaagcctg
    79381 atagggactt tctttttgaa ggaaaaccat ctgtcttata ttctacctta gaccgcgaga
    79441 aggaaaaaat agctcttaag ggcatacaag ctgggcatat tactgttagt tatttagcca
    79501 aagacaagaa aaacctaaag acagaaaaat attatcacgt cgacttagac gccttagcgg
    79561 tacatcccat ctctagtcgt gccaaaaagc gtcaattatt aaaagactat ctgctaaccc
    79621 atacgaagga agctaaactt gcaacgcttt atcaagcctt ttctagagat gtggtggctt
    79681 actttgtgac aaatcactta attcgtattg acgaacgtcc aattgatcgc agtgagtcct
    79741 attttgacca gatcaaacca tcttcttttt taaccttaaa tgaacagcag gcgtcagctg
    79801 ttactgaaat tgttgaacaa attgggaaac cgagtaaacc ttttttgatt gaagggatta
    79861 caggttcggg taagacagaa gtttatcttc atatcatcga agcggtttta aaacaggata
    79921 agacagccat tgttttagtg ccagaaattt cgttgactcc tcagatgacc tctcgtttta
    79981 tttcccgttt cggaaagcaa gtggctatta tgcattcagg cttatcagat ggtgaaaagt
    80041 ttgatgagtg gcgcaagata aaaacaggac aggctaaggt tgtggttggt gcccgctctg
    80101 ctattttctc cccgttagaa cgtattggtg caattattat cgatgaggag catgagtcaa
    80161 cttacaaaca agaaagtaat ccacgatacc atgccagaga ggttgccctg ctcagagcta
    80221 aacaccatca agcagttgtg gttatgggct ctgctacgcc aagtattgaa agccgagcaa
    80281 gggctagtaa gggggtctat cattttattc aattaacgca aagagcaaac cctcttgcta
    80341 agattccaga agtaacgatt gttgattttc gtgattatat tggacagcaa gcagttagta
    80401 attttacccc atatttgatt gataagatta aagaacgttt ggttaaaaaa gaacaagttg
    80461 tcttaatgtt aaatagacgt ggttattcta gttttgtgat gtgtcgggac tgtggttatg
    80521 tggacaaatg tcccaactgt gatatctctt taacccttca catggatact aaaactatga
    80581 attgccatta ctgtgggttt caaaaaccta tcccaattac ttgtcctgag tgtcatagca
    80641 atagtatccg ctattatggc actggaactc aaaaagcttt tgatgaattg caaggggtta
    80701 ttccagaagc taagatcttg agaatggatg ttgacaccac acgaaaaaaa agaagtcaca
    80761 aaaccatact agatagtttt ggtcgccagg aagcagatat tttgttaggg acgcaaatga
    80821 ttgccaaagg actggatttt ccaaatgtga ctttagttgg ggtactaaat gcagatacct
    80881 ccttaaattt gccagatttt cgagcgtcag aaaaaacgtt tcaattgtta acacaagttg
    80941 ctggtcgggc aggccgtgcc cataagccag gggaagtctt gattcaaact tataatcccg
    81001 atcactatgc tattcaatta gctaagaaac aagattttga agccttttac cgttatgaaa
    81061 tggcgattcg ccgtcagatg gcttatcccc cttactactt tacagtgggt attacgcttt
    81121 cgcatcgttt agaagcatcg gttgtaaaaa aagcttatca agttacagaa ctgctaaaga
    81181 gtcacttatc ggataatatt aaaatattag gaccgactcc taagccaatt gctagaacac
    81241 acaatttata ccactatcaa attttgttaa aataccgttt tgaagataac ctagaagaga
    81301 cgctcaatcg tattcttgat tggagtcaag aagcagataa tcgtcattta aaactaatta
    81361 ttgattgtga accgcaacaa tttttataag gtaaatcact aaatctagga tgatcagtta
    81421 tgatataata aaaatacaaa gaggaaagtc atgataaaat tattatttat gggaactccc
    81481 caattttcag caactgtatt gaagggcttg ctagataatc ctgcctatga gattttagga
    81541 gtggttactc agcctgatcg agctgttgga cgcaaaaaag atattaaggt aacacctgta
    81601 aagcaattag ctcttgagca tggaatatca atctatcagc cggaaaaatt atcaggctct
    81661 caagaactca ttgagattat ggaacttggt gcagatggta ttattacagc agcatttggc
    81721 caatttttac caacaatatt acttgattca gtttcatttg ctattaatgt acatgcatca
    81781 ttgttaccta aatatcgagg tggagcacct atccattatg ctattatgaa tggagataaa
    81841 gaggctggcg ttactattat ggaaatgata aaagaaatgg atgcaggtga catggtcgct
    81901 aaagcaagca ctccaattct tgaaaccgat aatgtaggta ctctttttga gaaattagcc
    81961 atcattggtc gagacttact tttggatagt ttgccagctt atctttctgg agaattaaaa
    82021 cctattcctc aagatcacag ccaagctact ttttccccta atatctctcc agaacaagaa
    82081 aaattagatt ggacaatgtc tgatcaagaa gtgttcaatc atattcgagg tatgaaccct
    82141 tggccggtag cgcatacgtt tttagagggg caacgtttaa aaatttacga agctcaatta
    82201 gctgaaggag aaggattacc tggacaagtt attgtcaaaa ccaaaaaatc tttggtaata
    82261 gctgctggtc aaggagccct gtcattgctg gttgtgcaac ctgcggggaa acctaaaatg
    82321 gctattgcag atttcttaaa tggccttggt agaaaacttg aagtgggtga tttcattggc
    82381 agataattgg aaaataagta ttcgaggaaa agcacttcta accttagaac atatttttga
    82441 tcagggggct tacacaaaca tagcgcttaa tcagcagcta agcaataaag ctttatctgc
    82501 taaggacaga gctctattaa ctgagattgt atatggtacc gtttcacgta aaattagctt
    82561 ggaatggtat ttagcccatt atgtcaaaga ccgagataaa ttggacaaat gggtttatta
    82621 tctccttatg ctcagtcttt atcaactgat ttatttagat aagttaccag cgcatgccat
    82681 tgttaacgat gcggttggta ttgccaaaaa tcgtggcaat aaaaaaggtg cagagaagtt
    82741 tgtcaatgct attttacgtc aattcaccag ccatcctctc ccagatatgg aaacaattaa
    82801 gcgtcgaaat aagtattatt cggtcaagta ttcactgcct gtttggttgg ttaaaaagtt
    82861 agaagaccaa tttggttctg acagatcagt ggccatcatg gaaagtttat ttgtgcgtag
    82921 taaggctagt attcgcgtga cagacccttt gaagctagag gaagctgctg aggctttaga
    82981 tgcagaacgt tcccttttat cagcaactgg tttaaccaaa gcgtcaggtc attttgcagc
    83041 cagtgattat ttcacaaatg gggacatcac tattcaagat gaaagcagtc agcttgtagc
    83101 tcctacttta aacattgacg gtgacgacat aattcttgat gcatgtagcg ctccaggggg
    83161 caagacctct catatagcat cttacttaaa aacaggtaaa gttattgcct tagatctcta
    83221 tgatcataaa ctagagttgg tgaaggaaaa tgctaaccgc ttaggagttg ctgataacat
    83281 tgagacaaga aaactagatg ccagagaggt tcatcgtcat tttgaaaagg atagctttga
    83341 taagattttg gtggatgctc cttgctcagg aattggtttg attcgtcgaa aacccgatat
    83401 caaatataat aaagaaagtc aagatttcaa tgctctacag gctatccagc tagagatatt
    83461 gtctagtgtt tgtcaaacct tgcgtaaagg tggtataata acatacagta cttgcactat
    83521 ttttgatgag gagaatcgtc aagtcatcga agcattttta caaagtcatc ctaattttga
    83581 acaagtaaaa ctgaaccata cacaagcaga tattgtcaag gatggttatc ttattatcac
    83641 accagaacaa taccagactg atggcttctt tattggacaa gtcagacgag tcttgtagta
    83701 ttttaggagg aaactgactg agtcagaaaa aacgtatgaa gatttcatta aaaacagaca
    83761 ttgggcaaaa acgctcaaac aatcaagatt ttatcaataa gtttgataat aaaaaaggca
    83821 tcactttagt gattttagca gacggtatgg gtggccacag agctggaaat attgccagtg
    83881 agatgacagt cacagacctg ggaagagaat gggtaaaaac agacttcaca gagttaagtc
    83941 agattagaga ttggttattt gaaactatcc agtcagaaaa tcaacggatt tatgatctag
    84001 gccaatcaga agactttaaa ggaatgggaa cgactgttga agcagtagct ttagttgaat
    84061 ctagcgccat ttatgctcat attggtgatt cacggattgg tcttgtccat gatgggcatt
    84121 ataccttatt gaccagtgat cattctttag tgaatgagtt ggttaaggca ggacagatca
    84181 cagaagaaga agctgctagc catccacaaa gaaatattat cactcaatct attggtcaag
    84241 cttccccagt agagcctgat ttaggagtga gggttttgga gccaggggat tatcttgtca
    84301 ttaacagtga tggtctaacc aatatgattt caaatgatga aattgtgact atcttgggca
    84361 gtaaggttag tcttgatgaa aaaaaccaag aaatgattga cttagctaac ctaagaggag
    84421 gcctagataa tattacaatc gctttggttc acaacgaaag tgaggacgtc gaatgattca
    84481 gattggcaaa ttatttgctg gtcgttatcg cattctgaaa tctattggcc gcggtggtat
    84541 ggcggatgtt tatttagcaa atgatttgat cttggataat gaagacgttg caatcaaggt
    84601 cttgcgtacc aattatcaaa cagatcaggt agcagttgcg cgtttccaac gagaagcgcg
    84661 ggccatggct gaattgaacc atcccaatat tgttgccatc cgggatatag gtgaagaaga
    84721 cggacagcaa tttttagtaa tggaatatgt ggatggtgct gacctaaaga gatacattca
    84781 aaatcatgct ccattatcta ataatgaagt ggttagaatt atggaagaag tcctttctgc
    84841 tatgacttta gcccaccaaa aaggaattgt acacagagat ttaaaacctc aaaatatcct
    84901 actaactaag gagggtgttg tcaaagtaac tgatttcggc atcgcagtag cctttgcaga
    84961 aacaagcttg acacaaacta attcgatgtt aggcagtgtt cattacttgt ctccagaaca
    85021 ggctcgcggc tccaaagcga cgattcaaag tgatatttat gcgatgggga ttatgctctt
    85081 tgagatgttg acaggccata tcccttatga tggcgatagt gctgttacga ttgccttgca
    85141 acattttcaa aagcctcttc catctattat cgaggagaac cacaatgtgc cacaagcttt
    85201 ggagaatgtt gttattcgag caacagccaa gaaattaagt gatcgttacg ggtcaacctt
    85261 tgaaatgagt cgtgacttaa tgacggcgct tagttataat cgtagtcggg agcgtaagat
    85321 tgtctttgag gatgttgaaa gtaccaaacc cctccccaaa gtggcctcag gtcccaccgc
    85381 ttctgtaaaa ttgtctcccc ctaccccaac agtgttaaca caggaaagtc gattggatca
    85441 aactaatcaa acagatgctt tacagccccc caccaaaaag aaaaaaagtg gtcgtttttt
    85501 aggtacttta ttcaaaattc ttttttcttt ctttattgta ggtgtagcac tctttactta
    85561 tcttatacta actaaaccaa cttctgtgaa agttcctaat gtagcaggca ctagtcttaa
    85621 agttgccaaa caagaactgt atgatgttgg gctaaaagtg ggtaaaatca ggcaaattga
    85681 gagtgatacg gttgctgagg gaaatgtagt tagaacagat cctaaagcag gaacagctaa
    85741 gaggcaaggc tcaagcatta cgctttatgt gtcaattgga aacaaaggtt ttgacatgga
    85801 aaactacaaa ggactagatt atcaagaagc tatgaatagt ttgatagaaa cttatggtgt
    85861 tccaaaatca aaaatcaaaa ttgagcgcat tgtaactaat gaatatcctg aaaatacagt
    85921 catcagtcaa tcgccaagtg cgggtgataa atttaatcca aacggaaagt ctaaaattac
    85981 gctcagtgtt gctgttagtg atacgatcac tatgcctatg gtaacagaat atagttatgc
    86041 agatgcagtc aataccttaa cagctttagg tatagatgca tctagaataa aagcttatgt
    86101 gccaagctct agctcagcaa cgggctttgt gccaattcat tctcctagtt ctaaagctat
    86161 tgtcagtggt caatctcctt actatggaac gtctttgagt ctgtctgata aaggagagat
    86221 tagtctttac ctttatccag aagaaacaca ctcttctagt agctcatcga gttcaacgtc
    86281 aagttcaaac agttcttcaa caaatgatag tactgcacca ggtagcaaca ctgaattaag
    86341 cccatcagaa actactgctc aaacacctta acaaagaata gttagtctca tgttttacta
    86401 gcatgccact aactattctt tttaactact cgtcatataa agatgcaatt ttccaggaat
    86461 tctgataaaa tacaaagaaa taaggggaaa ctaatgaaaa aatttcaatt ctttttactt
    86521 atcgagtgta tactgctagc tatgggtatc atgacaatct tggataatga tttatcaagt
    86581 tttatcctaa ttctagttct tatcttatta gcattacgct tttataacca agatagccgc
    86641 aataattttt tactaactgt cagtttattg tttctttttt taattttcat gcttaatccc
    86701 tacattatca tggctgttct tttggggata gtttacattt tcattaacca tttttcacaa
    86761 gtcaaaaaga agaatcgctt tgccttgatt cgtttcaagg aagaaaagat tgaagtgaac
    86821 aataccaagc atcaatggat tggtactgct aattatgaaa gtgattatta ttgttttgat
    86881 gacattaaca tcattcgaat ctcaggaaat gacacggttg acttaaccaa tgttattgtg
    86941 acaggaatgg ataatatcat tgtgatacgt aaaatttttg gaaatacgac tatattggta
    87001 cctattgatg tcactgttac tttagatgtt agttccatat acggaagcgt tgattttttt
    87061 agatgtcagc aatatgattt gcgcaacgaa tctattaagt taaaagaaac ggataaccaa
    87121 tcccttaaaa aagtcaaaat tatcgttaca actattgccg gaaatgttga ggtggatagg
    87181 cgatgaaaaa acgttactat gctcttgttt ggctctactc aaccattacc attttatcta
    87241 ttgtttttgt ggtcatggat aatttaggaa tcacttttaa ctaccttcgc aatcatttat
    87301 ggcaggtcga acgtctaggg ttttcgattt tattgttgat agtttctgtg actttattat
    87361 tgttgttatt gtggattatt atggatgata acagtaagcg taacatcaac caaaatctaa
    87421 aatatattct caataatcga cgactctatc tagatgagac atcagaaatc aatactaatt
    87481 taagtcgact ttctaaaaaa atgtctcacc tgactgctaa catgcaaaag aaagaaagtg
    87541 cttatattct tgatagccaa gaagttgtaa aacaagagcg caaacgaatt gcaagagatt
    87601 tacatgacac agtcagtcaa gagctattcg cttcgtcatt gattttatca ggaatttcga
    87661 tgagtttgga acaactggac aaaacacaat tacaaacaca gttaacaacg gttgaagcaa
    87721 tgttgcaaaa tgctcaaaat gatctacgca ttctcctttt gcatcttaga cctaccgagc
    87781 tagctaatcg gactttatct gagggccttc atatgattct taaggaatta acagataaaa
    87841 gtgatattga agtcatttat aaggaaacca ttgctcagct tcctaaaaca atggaagata
    87901 atctttttag aattgcccaa gaattcatta gcaacacgtt aaaacatgct aaagctagtc
    87961 gaattgaagt ttatctcaat caaacctcaa cagaattaca attgaagatg attgatgatg
    88021 gtgtaggatt tgatatggat caggtaaggg atttgagtta tggtctgaag aatattgaag
    88081 accgtgtcaa tgatttagca ggaaacctac atttaattag tcaaaaaggc aaaggagttt
    88141 ccatggatat tagactgccg atagtgaagg gagatgacga tgagtaagat aaaagtgata
    88201 ttggtcgatg atcatgaaat ggtccgcatg ggactcaaga gttttttgaa tttacaagct
    88261 gatattgatg tcgttggtga ggcctctaat ggacgtgaag gggttgattt ggcattggct
    88321 ttgaagccag atgttttggt tatggatcta gtgatgccag agttaggcgg tgttgaggca
    88381 actttagaag tcctaaaaaa atggaaagag gctaaggtac ttgtgttaac ttcctatcta
    88441 gataatgaaa agatataccc tgtcattgat gcaggagcga agggttatat gttaaaaaca
    88501 tcgagtgcgg ctgaaatttt aaatgccatt cgcaaggttt caaagggaga gttagctatt
    88561 gaaacagaag ttgacaaaaa aattaaggcg catgatcaac accctgactt gcatgaggaa
    88621 ctaacagcgc gtgagtatga tattttacac cttttagcta aagggtatga taatcagacc
    88681 atcgctgatg aactctttat ttccttaaaa accgtcaaaa cacatgtgtc caatattcta
    88741 gccaagttag aagttgatga ccgaacccaa gcggttgtct atgcttttcg acatcattta
    88801 gtcccccaag acgataatta atgctataat atagggtata gcaaattgga ggaaagtatg
    88861 gacgcaaaac taaaatacaa agctaaaaaa atcaaaatgg tgttctttga tattgatgat
    88921 accttgaggg tcaaagatac gggttatatg cctgagtcga ttcaaagagt atttaaggct
    88981 ttgaaggcaa aaggcatctt ggttggtatt gcttcaggtc gcgctcggta cggtgttcca
    89041 caagaagttc aagatttaca tgctgactat tgtgtcaaat taaatggagc ttatgtcaaa
    89101 gacgatgcta aaactattat tttccaagct ccgattccag cagatgttgt tgtggcctat
    89161 aaaaaatggg cggatgatat gggcattttt tatggaatgg cgggtcgtca tgaagcggtg
    89221 ttgtccgctc gaaatgacat gattagcaat gctattgata atgtctacgc tcaactagag
    89281 gtctgtccgg attataatga gtatcatgat gtttaccaga tgtggacttt cgaggacaag
    89341 ggagatggct tgcagttgcc tgctgaatta gcagagcacc ttcgtttggt cagatggcac
    89401 gataactcat cagatgttgt cttgaaaggt acgtcaaaag cgctaggtgt ttcaaaagta
    89461 gttgaccatt taggattaaa acctgaaaac attttggttt ttggagatga actcaatgac
    89521 ctagagttat ttgactatgc tggtatcagt attgcaatgg gagtatcaca tccattactg
    89581 caagaaaaag cagattttat cacaaaaaaa gttgaagaag atggcatttt atatgccttg
    89641 gaggaattag gattgattga caaagaatta cagtttccac aattggattt gccaaaccat
    89701 aaaggaccaa aagcaaccat taaaacaaac catggcgata tgaccctggt cttgttccca
    89761 gatcatgctc ctaaaacagt agccaatttc ttaggtttgg ctaaagaagg gtactatgat
    89821 gggattatct tccaccgtat tattcctgaa tttatgattc aagggggcga tccaacagga
    89881 acaggaatgg gtggacaatc catctatggt gaaagttttg aagatgaatt ttcggatgaa
    89941 ctctataatc tccgtggggc cttgtcgatg gctaatgcgg gaccaaatac taacggcagt
    90001 cagttcttta ttgttcaaaa tagtaagatt ccttatgcca aaaaagaact agaacgtggt
    90061 ggttggccgg ctccaattgc agcttcttac gctgcaattg gtggaactcc tcaccttgat
    90121 cgtcgtcata cggtctttgg tcagcttgtg gacgaaactt cattccaagt tttggatttg
    90181 attgctggcg ttgaaactgg cgcacaggat aaacctaaag aagaggttat cattgaaaca
    90241 atcgaggtat ttgactaatg aaaattggcg acaaactgca tggcaccatc actgggatta
    90301 aaccatacgg agcctttgtt gcccttgaaa atgggacaac aggtctcatt catatttcag
    90361 aaataaaaac gggatttatt gacgatattg atcaattatt ggctataggc aaccaggttt
    90421 tagtacaagt aattgatatt gatgaatata gtaaaaagcc aagtttatct atgcggacct
    90481 tagcagaaga aaaacagcat tttttccatc gtcatcgtta ttccaacagt cggcacaaga
    90541 ttggctttag gccacttgaa gagcagttac ctcagtggat tgaagaaagc ctgcagtttt
    90601 taaaaaagaa cactaaaaag agtctggaac cttagtagtt ccaaaccctt ttttatttcg
    90661 tcttaattac tggcacatca tagagttcag tcgataaata gcgttcgcca ttatctggta
    90721 aaatagttaa cacatgtttg ccttttccta attgtttagc gacttcaata gcggcgtaaa
    90781 gagcagctcc agaagaaatc ccaatcagga agccttcctt agctcctgtt agtcgagcag
    90841 tttctaaagc atctttcgat ttaacacgga taatttggtc ataggctttg gtatctaacg
    90901 tgtttgggat aaatccagct gatatacctt gaattttatg tggtccaggc tcttgaccag
    90961 ataagacagc agattcttca gcttcaacag catagataac agtttcaggg ctagcttttt
    91021 tcaagacatg tgaaacacca gaaagtgttc ctccagtacc aacaccagat acgaatgcat
    91081 ctaaagaaat ctccttaaaa gcttccaaaa tttcttgagc tgttgttttt tcatggatgc
    91141 ttggattggc agggttatta aattgcatag gcatccaagc acctagttct attgctaaag
    91201 tttcagcttt tgcaatagcc cctttcatac cttctgctcc aggtgttaag acaagctctg
    91261 caccataagc ctgaatgatt tgtcgtcttt ccaagctcat agtttcgggc ataacaataa
    91321 tgactcgata ccctttagca gcacctaccc atgcaagacc aatacctgta ttaccgcttg
    91381 ttggttcgat aataacgtca ccaggactta tcagaccttc agcttcagca gcttcaatca
    91441 tcgataaagc aatacgatct ttaacagaag atcctgggtt aaaagcttct aattttacat
    91501 aaacgtcagc agcttcgttt ggaattaaac agttaagttt gataataggt gtttgaccta
    91561 ctaattctgt tatagttttg taaattttag tcatagttta gcctcttttc ttgatacaaa
    91621 ataaatacgc tgtgtattta agtataagga gagatattat taaagtaaaa tacagattta
    91681 ctatcactct gataataaag ccatatcact tttgttgatg attaatcaca ggggaattca
    91741 ataactttgg atccgatttt ctcacagctt atctgaccat gataataatt tgttaaagta
    91801 tcaatgagat tttccacacg ttcagtatca caataaagtg ttgtctttat cgtgtctgaa
    91861 aacttagttt ctgtttcggt taaagccaat tgatctagca agttagagta tagttggtat
    91921 tggggataag aaagtgtgat ttctagtcct atctgctgtt taacttcaat gatgccaaat
    91981 cgtttaatag ctgttgcggt catgttagag tatgctctga ttagcccacc tgttcctaat
    92041 ttaataccac caaagtaacg cgttaccaca actactacat ttgttaaaca ctgcttttct
    92101 aacacagaaa ggataggtat tccagcagtt cctgaaggtt caccatcatc acttgagcgc
    92161 ttgatctgac tgttgttacc gataatcatc gcaaagcagg aatgattagc tttgtaatgt
    92221 tcttttttga tagcattaac aaaattctta ccatcttctt cagtgcttac tcgtttgata
    92281 tgacaaatga aacgagattt tttaatactt tcttcgaaaa aaccactggc tttgattgtc
    92341 ttaaaatgtt ccataaaaaa attgtacaag aaaagataaa aattagcaaa aaaaagcgaa
    92401 taataaagta tggagtctat tgaaaatagt tatggacgat tgttgctaga aagccagtta
    92461 ccagattcag caaagcagct tgctcaacct ttaaaaagtg tagtaatatc gagaggaaaa
    92521 atgatttgtc aacgttgtca ctatcagctt gatgaagaag cacgattgcc aaatggagcc
    92581 tattattgcc gattttgtct tgtctttgga cgaaatcaat cagataaatt gctctatgct
    92641 atcccaccta tgcattttcc aaaaggtaat tatctggttt ggagcggtca actaactgct
    92701 tatcaagaga tgatttccca acaattatta atcaatatgc aaaatcaaaa aacaacatta
    92761 gttcatgctg taactggtgc aggaaaaaca gaaatgattt atgctgcgat tgaggcggta
    92821 attaacacag gtggctgggt ttgcattgcc agcccaagag tagatgtttg tgtagaggtg
    92881 gctactcgtt tatcccaagc cttctcctgc agcatctgtt taatgcacgc agagtctctt
    92941 ccttatcaaa gagctcctat tatagtagcc acaacgcatc agctgctaaa atttcacaag
    93001 gcttttgatc tcttaattat tgatgaagta gacgcattcc cttttgtaaa taatattcaa
    93061 cttcattatg ctgcaagtca ggctttaaaa gaaggtggcg caaagatttt attaactgca
    93121 acttcaacta gaactttaga aagaaaggta aacaagggag aagtagtcaa gctgacattg
    93181 gcgagacgct ttcataatag gccattagtt attccgaaat ttataagaag ttttaatcta
    93241 tttaaaatga tacatcggca aaaacttcct cctaaaattt taaaatactt aaaaaaacaa
    93301 agaaaaacag gctatcccct tttaattttt ctacccacta ttataatggc agaatctgtt
    93361 acagcaatct taaaagagtt attgcccgcc gaacaaatag cttgtgtttc tagtcaatca
    93421 caaaacagga aagaagatat cacagctttt cgtcaaggga aaaaaacgat tttaattaca
    93481 acaacaattt tagaaagggg tgtgacattt ccccaaattg atgtttttgt tcttgggtct
    93541 catcatcgtg tctatagttc ccaaagtctt gttcaaattg ctggacgagt tggaagatct
    93601 atagacagac cagacggaac gttatatttc tttcatgaag gtatcagtaa agcaatgcta
    93661 ctagcaagaa aagagattaa agaaatgaat tataaagggt attcgcatga tctgtctact
    93721 atgtcaacaa attagtcaaa caccaataag tattacagaa atcatctttt taaggcgtat
    93781 ctcttcaccg atttgtcaac aatgtcaaaa aagctttcaa aagataggaa aaagtgtttg
    93841 tgcgacatgt tgtgcaaact cagatataat agcttgtcga gattgtctaa aatgggaaaa
    93901 caaaggatac aatgtaaatc atagaagctt atattgttat aatgctgcta tgaaagcata
    93961 cttcagtcaa tataagtttc aaggagacta tttattaaga aaagtttttg cagtagaact
    94021 tgccgatgtt attaccaagt actataaagg ctatatccca gtcccggttc ctgtaagtcc
    94081 cggttgtttt cgagaaagac aatttaatca agtgagcgct attcttgagg cagctaatgt
    94141 tagctacctt tctctttttg aaaagctaga taatactcac caatcttcca gaacaaaaaa
    94201 agagagatta ttagtagaaa aatcttatcg actactaaaa gtatcaaaca ttcctgataa
    94261 aatccttata gtagatgata tttatactac tggtagtaca attatcgctc ttagaaaaca
    94321 attggctaaa gtagcaaata gtgacattaa aagtttgtca attgcacgtt aaaacttgca
    94381 aattttcatg aaagcagtat aatagtatta aagataaagt aagcgcttca agcgcgagaa
    94441 agaggtatcc tatgattaag ttcagtattc gtggagaaaa tatcgaagtg acagaagcta
    94501 ttcgcgatta tgtagaatca aagcttacta aaatcgaaaa atattttgct aaagaccaag
    94561 aaatagatgc acgtgttaat ttaaaagttt atcgggagag atcttcaaaa gttgaggtaa
    94621 cgatcccatt agattcagta acccttcgtg ctgaagatgt ttcacaggat atgtacggct
    94681 ctattgattt agttgttgat aagatcgaac gccaaattag aaaaaataaa accaaaattg
    94741 ctaaaaaaca tcgcgaaaag gttccaactg gtcaagtctt tacaactgag tttgaagctg
    94801 aagaagtcga tgagatacca gaagtacaag ttgtacgtac taaaaatgtg acattaaaac
    94861 caatggatgt tgaagaagcg cgtttacaga tggaactatt gggacatgat ttctttatct
    94921 acactgattc tgaagatggt gcaacaaaca ttttataccg tcgcgaagat ggaaacctag
    94981 gattaataga agctaaatag ttcacaacta aaacctacag ggatgattat acgtatttta
    95041 aaagtgggaa aaagctattc atgtttagag gtgacatgat gaaaatgaga ttaagtgata
    95101 gatacgacag tcattatgct aacaaaagta tctattacag ttcctcttta agtaaatctt
    95161 acgtaaaatt agtttgaaaa aagattagtc ccaattctgg gctagaaatt ctcaaaaaat
    95221 atgaaaaagc gcgttttcag aatataaagc gcgttttttt ctttttccat ttaacaataa
    95281 aaaactcatg acgaacatgg tatcataaga gaatttttac tttttaaaca aaggtcaaag
    95341 actttctgac accaaataaa aatagaattt tttctaacta aaaataatag cacaatcttt
    95401 ggcagcaatc tgaattgttt ttccataaaa gatttaagca cttacgtttg cttaaaatat
    95461 aggttatgtg acgtgcgtgc aagatcagat tataacaaaa tagttccaaa taagcctatc
    95521 actgagaaag catcacagaa gggttaaact ttttttgtat tagcgtttta tcagtacaac
    95581 ctaacactta tttttataaa aaaacaaaaa agctattgac aatcttatac aagataacgt
    95641 atactaataa agctgttgta aatatggtaa agcaatagat gctatcagga aaaaatgtga
    95701 a
//
LOCUS       DAUCWU010000002        72908 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUCWU010000002 DAUCWU010000000
VERSION     DAUCWU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72908
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.002"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            61..273
                     /locus_tag="VGY24_000095"
     CDS             61..273
                     /locus_tag="VGY24_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ4283122.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(370..510)
                     /locus_tag="VGY24_000096"
     CDS             complement(370..510)
                     /locus_tag="VGY24_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283123.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(646..2139)
                     /gene="lysS"
                     /locus_tag="VGY24_000097"
     CDS             complement(646..2139)
                     /gene="lysS"
                     /locus_tag="VGY24_000097"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ4283124.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGYIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            2313..3215
                     /locus_tag="VGY24_000098"
     CDS             2313..3215
                     /locus_tag="VGY24_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ4283125.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            complement(3323..3946)
                     /locus_tag="VGY24_000099"
     CDS             complement(3323..3946)
                     /locus_tag="VGY24_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ4283126.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATATIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNASLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(4287..4766)
                     /locus_tag="VGY24_000100"
     CDS             complement(4287..4766)
                     /locus_tag="VGY24_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ4283127.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            complement(4856..5419)
                     /gene="thiT"
                     /locus_tag="VGY24_000101"
     CDS             complement(4856..5419)
                     /gene="thiT"
                     /locus_tag="VGY24_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ4283128.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     regulatory      complement(5464..5560)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5688..6536)
                     /locus_tag="VGY24_000102"
     CDS             complement(5688..6536)
                     /locus_tag="VGY24_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ4283129.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     gene            6861..7364
                     /locus_tag="VGY24_000103"
     CDS             6861..7364
                     /locus_tag="VGY24_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4283130.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            7348..7734
                     /locus_tag="VGY24_000104"
                     /pseudo
     CDS             7348..7734
                     /locus_tag="VGY24_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
     gene            complement(7780..8259)
                     /locus_tag="VGY24_000105"
     CDS             complement(7780..8259)
                     /locus_tag="VGY24_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ4283131.1"
                     /translation="MPNLYDFTIKAQNGNVLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFAWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(8252..10051)
                     /gene="pepF"
                     /locus_tag="VGY24_000106"
     CDS             complement(8252..10051)
                     /gene="pepF"
                     /locus_tag="VGY24_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ4283132.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIREKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNELNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIISYTEEMSHA"
     gene            10210..13008
                     /gene="ppc"
                     /locus_tag="VGY24_000107"
     CDS             10210..13008
                     /gene="ppc"
                     /locus_tag="VGY24_000107"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ4283133.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVISLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMSSSVGLNTSAWPSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            13148..14452
                     /locus_tag="VGY24_000108"
     CDS             13148..14452
                     /locus_tag="VGY24_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ4283134.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            14805..16001
                     /gene="tuf"
                     /locus_tag="VGY24_000109"
     CDS             14805..16001
                     /gene="tuf"
                     /locus_tag="VGY24_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ4283135.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            16242..17000
                     /locus_tag="VGY24_000110"
     CDS             16242..17000
                     /locus_tag="VGY24_000110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ4283136.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(17099..18334)
                     /locus_tag="VGY24_000111"
     CDS             complement(17099..18334)
                     /locus_tag="VGY24_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ4283137.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYVKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(18321..19547)
                     /locus_tag="VGY24_000112"
     CDS             complement(18321..19547)
                     /locus_tag="VGY24_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ4283138.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDLDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(19547..20356)
                     /gene="yidA"
                     /locus_tag="VGY24_000113"
     CDS             complement(19547..20356)
                     /gene="yidA"
                     /locus_tag="VGY24_000113"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ4283139.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGIHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(20812..22113)
                     /locus_tag="VGY24_000114"
     CDS             complement(20812..22113)
                     /locus_tag="VGY24_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ4283140.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPKQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            22195..22581
                     /locus_tag="VGY24_000115"
     CDS             22195..22581
                     /locus_tag="VGY24_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ4283141.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            22811..25492
                     /locus_tag="VGY24_000116"
     CDS             22811..25492
                     /locus_tag="VGY24_000116"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEQ4283142.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAQEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(25576..26571)
                     /locus_tag="VGY24_000117"
     CDS             complement(25576..26571)
                     /locus_tag="VGY24_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4283143.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(26635..28542)
                     /locus_tag="VGY24_000118"
     CDS             complement(26635..28542)
                     /locus_tag="VGY24_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ4283144.1"
                     /translation="MSLAFTRFKETVNPDFCRNYLLDYQTDSYADQKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQKLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFNALTKQEESLIL
                     ASLEKQLQFLHANYLDKYSLSNWGILQTTAILLADAYYGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSLCLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQIDSSWAYLSYPKSNYCHLRQNGHIYF
                     IDGSYQTQFSDRNNYQHDRQILILPPGILLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKLIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(28629..29450)
                     /locus_tag="VGY24_000119"
     CDS             complement(28629..29450)
                     /locus_tag="VGY24_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4283145.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            complement(29437..30219)
                     /locus_tag="VGY24_000120"
     CDS             complement(29437..30219)
                     /locus_tag="VGY24_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ4283146.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWSFAILGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(30238..30726)
                     /locus_tag="VGY24_000121"
     CDS             complement(30238..30726)
                     /locus_tag="VGY24_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ4283147.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(30762..31961)
                     /locus_tag="VGY24_000122"
     CDS             complement(30762..31961)
                     /locus_tag="VGY24_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ4283148.1"
                     /translation="MTRPLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKDLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(31961..32398)
                     /locus_tag="VGY24_000123"
     CDS             complement(31961..32398)
                     /locus_tag="VGY24_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4283149.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            32730..33524
                     /locus_tag="VGY24_000124"
     CDS             32730..33524
                     /locus_tag="VGY24_000124"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ4283150.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            33549..34190
                     /locus_tag="VGY24_000125"
     CDS             33549..34190
                     /locus_tag="VGY24_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ4283151.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            34219..35220
                     /locus_tag="VGY24_000126"
     CDS             34219..35220
                     /locus_tag="VGY24_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ4283152.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACEAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILAREAKKRDI
                     CISFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRKKADATMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDVQR"
     gene            35225..35860
                     /locus_tag="VGY24_000127"
     CDS             35225..35860
                     /locus_tag="VGY24_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ4283153.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            36157..36807
                     /locus_tag="VGY24_000128"
     CDS             36157..36807
                     /locus_tag="VGY24_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ4283154.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            37503..38627
                     /gene="queG"
                     /locus_tag="VGY24_000129"
     CDS             37503..38627
                     /gene="queG"
                     /locus_tag="VGY24_000129"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ4283155.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPERLLESAKTIISIGVAYPHKLPQQPQKTLYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSQKELELIRRKWQV"
     gene            38696..39794
                     /gene="prfB"
                     /locus_tag="VGY24_000130"
     CDS             join(38696..38767,38769..39794)
                     /gene="prfB"
                     /locus_tag="VGY24_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ4283156.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            39813..40505
                     /gene="ftsE"
                     /locus_tag="VGY24_000131"
     CDS             39813..40505
                     /gene="ftsE"
                     /locus_tag="VGY24_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ4283157.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            40498..41427
                     /locus_tag="VGY24_000132"
     CDS             40498..41427
                     /locus_tag="VGY24_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283158.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(41736..42434)
                     /locus_tag="VGY24_000133"
     CDS             complement(41736..42434)
                     /locus_tag="VGY24_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4283159.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            42602..43366
                     /locus_tag="VGY24_000134"
     CDS             42602..43366
                     /locus_tag="VGY24_000134"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ4283160.1"
                     /translation="MSKVAVVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKIVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            43474..45975
                     /locus_tag="VGY24_000135"
     CDS             43474..45975
                     /locus_tag="VGY24_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ4283161.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFAR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            46310..47503
                     /locus_tag="VGY24_000136"
     CDS             46310..47503
                     /locus_tag="VGY24_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ4283162.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            47524..48870
                     /gene="asnS"
                     /locus_tag="VGY24_000137"
     CDS             47524..48870
                     /gene="asnS"
                     /locus_tag="VGY24_000137"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ4283163.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            49283..50173
                     /gene="rapZ"
                     /locus_tag="VGY24_000138"
     CDS             49283..50173
                     /gene="rapZ"
                     /locus_tag="VGY24_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ4283164.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIIPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            50170..51147
                     /locus_tag="VGY24_000139"
     CDS             50170..51147
                     /locus_tag="VGY24_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ4283165.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNENDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCKAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            51144..52055
                     /gene="whiA"
                     /locus_tag="VGY24_000140"
     CDS             51144..52055
                     /gene="whiA"
                     /locus_tag="VGY24_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ4283166.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSFIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            52188..53585
                     /locus_tag="VGY24_000141"
     CDS             52188..53585
                     /locus_tag="VGY24_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ4283167.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTSKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYSHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRNFALAWCQKTYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            53737..55284
                     /locus_tag="VGY24_000142"
     CDS             53737..55284
                     /locus_tag="VGY24_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ4283168.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            55432..56153
                     /locus_tag="VGY24_000143"
                     /pseudo
     CDS             55432..56153
                     /locus_tag="VGY24_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            56172..57371
                     /locus_tag="VGY24_000144"
     CDS             56172..57371
                     /locus_tag="VGY24_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ4283169.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCNSANRNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQRYLFESVASTDLVAAP
                     DYYLVEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERQLQELADLGFERLVYLGSASLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(57468..57728)
                     /locus_tag="VGY24_000145"
     CDS             complement(57468..57728)
                     /locus_tag="VGY24_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ4283170.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(57843..58784)
                     /locus_tag="VGY24_000146"
     CDS             complement(57843..58784)
                     /locus_tag="VGY24_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ4283171.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWMSQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            59178..59627
                     /locus_tag="VGY24_000147"
     CDS             59178..59627
                     /locus_tag="VGY24_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ4283172.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            59803..60087
                     /locus_tag="VGY24_000148"
     CDS             59803..60087
                     /locus_tag="VGY24_000148"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ4283173.1"
                     /translation="MRLEEIRQEINSIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLKGGDTND"
     gene            60080..61342
                     /locus_tag="VGY24_000149"
     CDS             60080..61342
                     /locus_tag="VGY24_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ4283174.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNSYLRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            61457..61804
                     /gene="rplS"
                     /locus_tag="VGY24_000150"
     CDS             61457..61804
                     /gene="rplS"
                     /locus_tag="VGY24_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ4283175.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            62051..62122
                     /locus_tag="VGY24_000151"
     tRNA            62051..62122
                     /locus_tag="VGY24_000151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62084..62086,aa:Arg,seq:cct)
     gene            62820..63389
                     /locus_tag="VGY24_000152"
     CDS             62820..63389
                     /locus_tag="VGY24_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ4283176.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            63390..65342
                     /gene="gyrB"
                     /locus_tag="VGY24_000153"
     CDS             63390..65342
                     /gene="gyrB"
                     /locus_tag="VGY24_000153"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ4283177.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            65710..67434
                     /gene="ezrA"
                     /locus_tag="VGY24_000154"
     CDS             65710..67434
                     /gene="ezrA"
                     /locus_tag="VGY24_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ4283178.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(67566..68024)
                     /locus_tag="VGY24_000155"
     CDS             complement(67566..68024)
                     /locus_tag="VGY24_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ4283179.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            68251..69558
                     /gene="eno"
                     /locus_tag="VGY24_000156"
     CDS             68251..69558
                     /gene="eno"
                     /locus_tag="VGY24_000156"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ4283180.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(70040..71069)
                     /locus_tag="VGY24_000157"
                     /pseudo
     CDS             complement(70040..71069)
                     /locus_tag="VGY24_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(71336..72877)
                     /locus_tag="VGY24_000158"
     CDS             complement(71336..72877)
                     /locus_tag="VGY24_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4283181.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLIEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHVVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYHDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNPFHKDKWT
                     PKKVKELFELVMTKTIGKTLKASLRPLLGDNILNSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
BASE COUNT        22189 a        12980 c        15340 g        22399 t
ORIGIN      
        1 tgaaaaaaag atgatttttt ccactttaat ttcgaatagt atagatgagg aggaaaacta
       61 atggcagaat ttacattcaa cattgaggaa catttactca ccttatcaga aaatgataaa
      121 ggttggacga aagaattgaa ccgcgtcagc tttaatggcg cagaagcgaa atgggatatc
      181 cgcacgtgga gtccagacca tacgaaaatg gggaaaggca ttactcttac taatgaagaa
      241 tttaaaacca tcttagacgc ttttcgtaag taatgctacc ttaatcgaaa cataatccct
      301 cgttcgccct tgtcctctta atctggtgat gacatcgcta ggttaagagg acaggggcga
      361 atagaaggat tatttttttg aattgaggcg gtcagcaagg ccgacaagta ccaaaattaa
      421 gcctagccca agaaaaatcc atttggcagc taaaaggcca taaagcaaga agatagcagc
      481 actaatgtag gagatatatt ctttggtcat cttgttaagt cactttctat aagctactgt
      541 taataaacca gtcttcatgg taatgaagac tggttagaga taataaaacg ttatgttata
      601 ttgtcactta tcggttttaa gatatgaata caaatcttaa aaaagttaag gtttcatcgt
      661 tgggaataaa agcacatctc tgattgttgt tgtgtttgtt aataacatgc aaagacgatc
      721 aatcccgatt ccgagtccac cggttggcgg cataccatat tcaagagctt caacgaagtc
      781 gtagtcaatg ccagttgctt cgtcatcacc aagttcttta gcttgtgctt gtgcttcaaa
      841 acgagataat tggtcaatgg gatcatttaa ctctgtgaaa gcattggcgt attctttggt
      901 catgatgaaa agttcgaagc ggtcggtgaa gcgggtatct tctggattct tcttagccaa
      961 aggtgatact tcaacaggat gaccaaagac aaaggttggt tggaccaagg tttcttcaac
     1021 gaattcttcg aagaaagcat tgatgatata tccgacactt gtgaaatgtt tttcgagtgg
     1081 gacctgtttt tctttggcaa gtgcaatagc ttcttcgaca gtcatttcag gccagaaatc
     1141 aacacctgtc acttccttga tagcatctac catgtggaca cgtttgaaag gttcattgat
     1201 acggatttca gttccttgat aatcaatagg cccatcacct ttaacagctt tagcagcatg
     1261 ttggataatg ccttctgtca ggttcatgat atcaagatag tctgcatagg cttggtaaac
     1321 ctcaatagaa gtgaactcag gattatgagt agcatccatt ccctcgttac ggaagatacg
     1381 accaatttca tagacacgtt ccataccacc aacaatcaaa cgtttcaaat gaagttctgt
     1441 tgcgatacga agcaccatat caatgttttg agcattatgg tgagtaacga aaggacgagc
     1501 agcagcacca ccagcctcgt tatgaagcac aggcgtttct acttctagga aatcaagacc
     1561 gtctaaataa cgacggattt ctgaaatcat tttggaacgg gtcacgaagc gatcaaagct
     1621 ttcacgattt gaaattaaat caaggtgacg cttacggtaa atggtttcaa tatcagttaa
     1681 tccgtggaat ttttcaggaa gaggacgaag tgatttagaa aggtgagtca acttggttgc
     1741 cttgattgac aactcgccca tgtctgtacg catgacttcg ccttcaacgc cgataaagtc
     1801 gcctaaatcg gcttttttga agatttcata gttgtcttcg ccaacagagt ccttgcgaac
     1861 ataaagttgg atttggcctt cacggtcttg aagatgagca aaaccaacct tacctttgcc
     1921 acgttttgtc atcaagcggc cagcaacaat agctgtttcg tttaactcat gtaattcttc
     1981 ttttgtttta tcagcatact tttcttttaa ttcagcagaa ttggctgtgc ggtcaaagcg
     2041 tttaccgaaa ggatcaattc cttgttcagc cagcgccgtc attttttcac gacggacaat
     2101 ctgttggtca tttaattctt cgatgtgttg attagacata ttttcctcca atacgatttc
     2161 ctttccattg tatcataaaa gaggctagaa tacacctata tttcgttgga aaatgcgact
     2221 gcagcggggc acttttttga aaataaaatt gaaaagccta atggaaaata gtataatgat
     2281 tgggatatta aactagaaaa gaaaagagac tcatgattac tgctattgtt tttgatgtgg
     2341 atgacacaat ttacgaccag caagcgccct atcgaatcgc catggaaaag tgcttcccag
     2401 attttgatat gtcagtaatg aatcaagctt atattcgctt tcgtcattat tcagatgttg
     2461 gattcccaag agtaatggca ggggagtgga ccacagaata tttccgattc tggcgttgca
     2521 aggaaacact gcttgagttt ggctatcgag aaatcgacga agctgcggga gtacattttc
     2581 aagaagtcta tgagcacgaa cttgagaaca ttaccatgtt agatgagatg cgcatgaccc
     2641 ttgacttttt aaaatcaaag aatgttccta tggggattat taccaatggg ccaacagaac
     2701 atcagctgaa aaaagttcgt aaattagggc tttatgatta tattgatcct aagcgtgtca
     2761 ttgtgagcca agcaacaggc tttcaaaaac cagaaaaaga aatttttaac ctggcagcag
     2821 aacaatttga catgaaccca caaaccacac tatatgttgg ggactcttat gacaatgata
     2881 tcatgggtgc ctttaatggt ggttggcatt ctatgtggtt taatcataga ggtcgtcagc
     2941 taaaaccagg aactaagcct gtttatgatg tggctattga taactttgaa cagctctttg
     3001 gtgccgttaa agtactcttt gatttacctg ataataaatt tatttttgat gtcaatgaca
     3061 agaaaaatcc aattttacag atgggaatca ataatggctt gatgatggca gcagagcgtt
     3121 tacttgaaag caacatgagc attgacaagg tggttatctt gcttcggtta accaagcaac
     3181 aagaaaaagt tttgcgctta aaatatgcca gatagatgaa accataatag cccactagat
     3241 aataataaaa aaagctagca aagtgagtgt cttgtcagta agagatcaat gtgttagctt
     3301 tttttattat tttctagtgg ttttacacct ctagaaaaga tttatcattc caaattaaac
     3361 agtcataagt taaataatct ttagtttcaa gaatagtaag actggcatta tctaagccac
     3421 ctttagcgag taaaagagca ggttcaaaac ctaataatga gcgaatagtt gctgttaagt
     3481 tagcgccgtg tcctacaata agaacgtttt gataatgttt atctttaaac gattggataa
     3541 gatggcagac acgttgagtc gtttggtaaa ttgattcagc ctcgaactga tctggtttga
     3601 actgggctag attctcccga aaagccagca tttgttgggg atatatagct gccattgttg
     3661 ctattttagc cccttccaat ttacctaatc tccactcccg aagctggtcg gtgtggtaaa
     3721 gttttggctg ttggtcaaaa gcatctaaga taatagttgc tgttgccata gctctttgaa
     3781 ggtcgctagt ataaacggca tcaaaagcca ccttagctaa ctctttgcca agtaagtgaa
     3841 tttcgtcttt tgcctcttca agtaatggcg aatcgccccc agcgccttga aagcgacctt
     3901 ctaaattcca aagggttttc ccatgtctta caaaatataa tttcatttaa gcctcctttt
     3961 agagataact gatgcgagcg ctaaaaataa aaaagtaacc agaaaaagtt taggcatcac
     4021 caagcaaaaa taatacctat agtgaaaaga taaagtaaaa aagcgtagta taagtgtata
     4081 atagctaggt tctgattaaa gtgtaaagcc catattagat ctaagacaaa tcgacctgaa
     4141 tttgtcgtga tatcaagtct ctacaaaaga aaaagattaa caggatcaat gttaaagcag
     4201 ttgagatcaa aataatacta aacaaagact agcttaatca gggcgaaaaa cttaaaaaag
     4261 aaaccagata aagttggcag tagtgttcat tttctcttct ttaaatcagc aaagcttgcg
     4321 ccaacgaaat cagctagagc ctggctagaa atctttatag aacgcccaac ttcaccagct
     4381 gaaacgatta tttgaccttt ttccagtgca gtttggtcaa taaaaatagg atatgaatgt
     4441 ttttgacgaa tcccaacagg attattggca ccgtgaatat agcctgttgt cttttgcaag
     4501 tctttttgag gaaccatgga cacttttttg ttacctgata cttttgccaa ttgtttttct
     4561 gataagtgtt ctgttaatgg aataatgcca attagaggac ctgtttgatc accagtcaga
     4621 gcaagtgtct tgtaaatatc tgatggttga agatcatctg gaaagtcgcc ttctaaagca
     4681 ttaagcttga gtccttgatg agcaatatta gccttatcga ggatttgttc tactaaggtt
     4741 tttttgagtt ttgttttttt agccatatca agtccttacc tttatctaaa cataaaaaat
     4801 ggtataacaa cattattata ccattttttt aatgtaagat tcaaatgact aaaaattagt
     4861 ctttgggatc aaataattga ggatgaattg gtaaagcaat cattaacgct aaacatgtta
     4921 ttaaaaaagt taggacacca gctgtaccat taacgctaaa agaataccag acagcggaca
     4981 tgcctttagg ggcgtaactg ccccagaaaa tgattcctgc aaggaagtgc cacaagtaac
     5041 gtactgtaac ggctagtaaa ctagccatta ttgcaagaaa aacggcaaaa aaggtttgtt
     5101 gtcttcttaa agttttaatc aaactatcag agaaactacc ggctaatccc atagaggtaa
     5161 aggctaagat atattcaata aagacttggg acatgctgag atagtaaact tttccaagta
     5221 caaagtgaag cagcccccaa atgagacccg ccaacatgcc gtactttaac ccacggcgca
     5281 aagagaaaat aaccagtgca atagcaccat aagaaggact aaaccagcca gcaaaatctg
     5341 gaataaatga aagggccatc gccaatgcag caaaaatagc agcttcgata aggtatttga
     5401 cattggtgtt tggtgacata aaaaactcct tttgtgcaca aaaagagtat cgctttccaa
     5461 tcattgatta ttggtcacaa tccctacgct tgtactaaca agatcaggtc ctcaggattt
     5521 cgcaattgcg atctcagcca aagcactcct ttgtgaaata ttatagtgct ttcattatat
     5581 gatgagataa tctccctgtc aaggataagg gggggtaaat tattgaccta gagtttttct
     5641 tggtaagatg taaactataa aaaaagatta gccatgatgc taacctctta attgttggag
     5701 ttgccaaata acttctcaaa cgttttttta gtgtcttttg ttacggcaat ttgatttaaa
     5761 tctaaagcat tgttaaaacc actgagataa ccttgtgagg tatattggtg taaatcgtaa
     5821 tctaaggttg tattaggagc agcctcataa tagccagaat cactaccata agtcggaatc
     5881 catacagcat cgaaaccttt tgttgaaata tcttgttctg ccataaaata agttccaata
     5941 tagagaccaa cattttcagc accaagtttt ttaagctctt ctcggaaggc tgttacgcct
     6001 ttattcatat ctttcatagt ggcttcttcg acatcaatcc agtagtaagt tggattatag
     6061 ggagcagcgt ttttgtagaa agctcgggcc tcttctttca tctctttagt actacgacca
     6121 agtgcgtaac tgtaaacagc tacaggaaca tttcgttttt gaaattcttt gatatgcgtt
     6181 ttaaaagatt tgtcaattcc agttgtaaaa gcagcgttgt tatgagctgt aatttgcgat
     6241 cctccataga cacgtacaat tgcgccagag atgtgacgtg acaatgtgtc ataatcaatt
     6301 tcttcaggga gttgccagcc ggaaacatca acaataggat ttaaaataaa gtcttcggtt
     6361 tcagaagtgc tagtttttgc tttactagga ttactggttg caataggaat atgactctta
     6421 gcgtcttcaa cttctttttg tttcgcatga ttagcttgtc tttttccgat aatcaatacc
     6481 attgccagta aaataaagaa gactagcaca acaataggtt ttatttttct tctcattaac
     6541 tgttattgta ccctaattac taaaaaaaat caaaatatcc taactgtttt cttatattgt
     6601 cttgcatttt aaggtattat cttttagaag ttacaaatga gatcctctct aactatgtca
     6661 agtttatgtc actatctctt atagttggta gctatattct ttacaatatt agtttagtct
     6721 ttgactatat catgacagtc agcaagtact gttttgttat ttaattctca tgttattatc
     6781 atttgataaa taagtgagct tcaaactggt aaacgctctg taaatctgtc ataatagaga
     6841 aaaaaacgag gaagaagttt atgtcagcta agcttatgag tcgcattgct atcatgtcag
     6901 ccctatgtgt tgtgttgaga atggttttta gcagcctgcc taacgtgcag cctgtgacgg
     6961 catttttact cagttatctt ctttactttg ggttagccga ggctgtatta gttatgatgt
     7021 tgtgcttgtt tttatcagcc tttttgctgg gatttggccc ttgggtcttt tggcaagtta
     7081 cttgttttgt gcttgttctt ttattatggc gttttgtctt gtaccctttg tctcagcaat
     7141 tccccaaata tcaacttggc tgtcaggctt tcttggtcgc cttatgtggc ctcctttatg
     7201 gggtgttgat agatacttgt tttgcgtatt tgtatagtat gccttggtgg agctatgttt
     7261 tagcagggat gccctttaat atagcgcatg ctttatcaac tctcgtcttt tttccagttg
     7321 tgatgatgtt atttaggagg ttaatcggtg aacaaaagat ttaaaacagg atttctagcg
     7381 cttgttgcta tgctacttgt tgcttgctcg caaggaacaa aacaaataca gacgactcca
     7441 agcgttccaa aagcagacca tcacgttcgc ttagttgtca aagaagatac caataccatt
     7501 gatgaaaaag tttcttttgg aaaaggagat acggtgcttg agattctcaa agataattat
     7561 gaggttaaag aaaaagatgg ttttatcaca gcaattgatg gtatcgagca agacacgaaa
     7621 gctaacaaat actggctttt ttaggtcaat ggaaagatgg cagataaggg tgccgatcaa
     7681 attaccgtta aagatggtga tagcattgaa ttttatcaag aagtttttaa atagtaacta
     7741 taatataaaa tagacttggc acgccaagtc tattttatat taaccaagaa gtgctttaag
     7801 gtcttcttca attagtttag gatctgtctt tgaggaatag cgtttaatga cttgaccatt
     7861 ttggtcaatg agaaatttag tgaaattcca ttcgatgcgt ttcccaaggg gaccactttt
     7921 ttcttctttt aaccaggcaa acaaaggatc agcgtcttta ccattcactt tgatttttgc
     7981 aaagcgaggg aaagtagtgt gataggtcag actgcaaaag tgattaatct cctcagcatc
     8041 tcctggagct tgatttaaaa attggttgca aggaaaatct aagacctcaa atcccttgtc
     8101 atggtatgtg tcatatagtg cttgcaaggc ctggtattgt ggggttaatc cacacttggt
     8161 agctgtgtta acaatcaaaa caaccttttt tttataagca gctaaggaaa ggacatttcc
     8221 gttttgagcc ttaatcgtaa aatcatagag attaggcatg tgacatctcc tcggtgtaac
     8281 tgataatttg gtcaacggta tcactcaaga attgaatggt atctcgaaga ggcttttcag
     8341 tggcaatgtc tgccccaatg agcattgcag tatcaagagg cgtacggctg ccacctgatt
     8401 ttaggaagtc aagccattct tttgccccat ctgggttgtt tttaaggtta aggtaaccag
     8461 ctgttgacat aacaagccca gcagaatagg tataactata taagcccatg tagtaatgcg
     8521 cttggcgcat ccaggtaaga gctgcgtcat cgtcaatgtc aacggcatct ccccagaagt
     8581 ctgttaagac ctctttcatc attgcattta attggtcagc tccaaaggta ccgccttctt
     8641 caatcagggt gtaaacctta cgttggaaag ccgcttccaa taggtgagtg atgaagttat
     8701 gaaagtaagt atctgttaaa cggtgagcaa gagcaaaacg tttttgacga gggtcatcaa
     8761 actgatgttc caagtaatca ctaagcatca attcattaaa ggtcgatggc gcttcgacgt
     8821 aataagttga catatgggtg ttgaagtaac tttggtgatt atctgaaaaa atgaattgac
     8881 cagagtgacc gatttcatga ataagggtat aaacatcaga cattcttcca gtccaactca
     8941 ttaagacata cggatgaacc ttgtagggat cagcggcata gccaccagaa tccttattgg
     9001 cgttagcagc aaagtcaacc cagcgttctg tctgataacg ttcgatttct ttcgtgtatt
     9061 cttgccctaa tggggctaga gatttcatca cgaggtcata ggcaccatca atgctgacct
     9121 ctggattgag ctcattatca atatctagtt tccaatcagc aaaagtcatt ttctcaagac
     9181 cattgacttg ggcgacatgt tttaagaatt tctgagcaac cggtccaaat tcagtcatga
     9241 tgagatcaat ttggcggtcg aacaagctac gatccacttc ctgttcagct aggaggtaat
     9301 caaagacaga agcgtagcct ttcatatccg ctaacaattt ttcagattta accttggcta
     9361 agtaagccgc agcagctgta ttttggtgtt tacggagacc ttttgaaaaa gagcggaagg
     9421 ctttttcacg aatctcagca ttttcgtgat tttgatagaa attttcgtaa gaaacaaagc
     9481 tatttttata ggttttacca tccacctcaa aatcctccat gtcaaagtca ccagcccgca
     9541 ttttggtgta aatatcatag ggcgcattga taacctcgcg taagttagcc aaagcttttt
     9601 ccacatcagg acttaagagg tgttcttttt gaatttttgc catgcgaata gctgcgctga
     9661 aataagggtt cttttctaat gtatctaaca cgtcaaggtc ggcattggct aaggcggtgt
     9721 caaagaagct aagggcaacg ctagctttag tcatgaaatc atcgccagcc tgagcaattt
     9781 gagcaaaact ttcatctgaa aagtcagtgg tttgaggcat aaaggcataa gtgccaatat
     9841 gactcatttg aatataaata tgctcaattt ctattaaggc ctgagtgaaa tcgtccaccg
     9901 atgttaagcg gccttcataa ttgcgcttaa acaggtcaat atcttgtaag gctttttcaa
     9961 tagcgagtaa aaaatcttgg cggtctttgt agagcgctgt tagatcccaa agttcatttt
    10021 cagggaattc tgaacgtttt ttcaattcca tagttgtctc cttaacaata tagtcaagac
    10081 tagtataaca gaattttagt ggaatatgga tactaaacct atccaaacat tctttgaaat
    10141 aaagcgctta cttttgttaa aaaacaaacg ttatgaacat gatcactttt atttttggta
    10201 aaaagtggta tgatagaaga aactagcaaa gaaggagatg gtcatttgcc acttaaaaag
    10261 ttagagagta gtaacaatca agctatcatt gctgaagaag ttgctctttt gaaagaaatg
    10321 ttggaaaata tcacacgacg gatgattgga gatgacgcgt ttacagttat cgagtctatt
    10381 atggtgttat ctgaaaaaca agattacata gaattagaaa aagttgtcgc caatatttca
    10441 aatcaagaaa tggaagtgat ttccaggtat ttttctattt tacccttgct aatcaatatt
    10501 tcagaagatg tcgatttagc ctatgaaatt aattatcaaa ataatacgga tactgattat
    10561 ttaggaaaat tagccctaac catcaaggat cttgctggaa aagataatgg taaagacatt
    10621 ttggagcagg ttaatgtggt tcctgtttta acagcacatc cgacgcaagt acaacgtaaa
    10681 acgattctgg aattgacaac tcacattcat aaacttctgc gcaaataccg tgatgctaag
    10741 gctggtgtga ttagtcttga gaaatggcgt caggagctat atcgttatat tgaaatgatc
    10801 atgcagacag atattattcg cgagaaaaag cttcaggtga aaaacgagat aaaaaatgtc
    10861 atgcagtatt acgatggttc cctaattcag gctgtgacca agttgacaac agagtacaaa
    10921 aacttagccc aaaaacacgg tctagagtta gacaatccca aacccattac catggggatg
    10981 tggattggtg gggaccgtga tgggaacccc tttgtcacag ctgaaacctt gtgcttgtca
    11041 gctacggttc aaagtgaggt tatcctcaat tactacattg acaaattagc agccctttac
    11101 cgaacatttt cactgtcgtc aaccttggta caacccaact cggaagtgga acgtttagcc
    11161 agtttgtctc aggaccaatc gatttaccgt ggaaatgaac cttatcgcag agcttttcat
    11221 tatattcaat cgcgtcttaa acaaacacaa attcaactaa ctaatcagcc agcagctcgt
    11281 atgagtagta gtgtgggact aaacacatct gcttggccct caccagctag tttagaaaat
    11341 cccatcttag cctatgactc tcctgttgat tttaaagctg atttaaaggc tattgagcag
    11401 tcgctacttg ataatggcaa ctccgctttg attgaggggg atttgcgaga agtcatgcaa
    11461 gctgttgata tttttggctt cttcttagca agcattgaca tgaggcaaga ctccagtgtc
    11521 caagaagctt gtgtggcaga gttattaaaa ggagccaata ttgttgacga ttatagctct
    11581 ctttcagaaa ctgaaaaatg cgatgttctc ttacaacaat taatggagga gccaaggact
    11641 ttatcctcag cggcagttgc taaatcagac ctattggaaa aagaactggc tatctatact
    11701 acggcacgag aattgaaaga taagcttgga gaagaggtta ttaaacaaca tattatttca
    11761 catacagaaa gtgtctctga tatgtttgag ttggccatca tgcttaaaga agtgggattg
    11821 gttgaccagc agcgggctcg tgtgcaaatt gttcccctct ttgaaaccat tgaggattta
    11881 gataatgccc gtgacatcat ggcagcctat ttaagccatg acattgtcaa atcttggatt
    11941 gcaacgaatc gtaattacca agaaatcatg ttaggttatt ctgacagtaa taaagatggt
    12001 ggctacttgg catcgggttg gactctttat aaagctcaga atgaattaac ggctattggt
    12061 gaggaacatg gagtgaaaat caccttcttc catggtcgtg gaggaacagt tggtcgagga
    12121 ggcggccctt cttatgatgc cattacttca caaccttttg gttccatcaa agatcggatt
    12181 cgtctgacag agcaagggga aattattgaa aacaaatacg gcaataaaga tgtggcttat
    12241 taccatttag aaatgttgat ttcagcttct atcaatcgta tggtgactca aatgataaca
    12301 gatcctaatg agattgatag ttttcgagaa atcatggata gcattgttgc tgatagcaac
    12361 accatttatc gaaaattggt ttttgacaat ccccatttct atgattattt ctttgaggca
    12421 agcccgatta aggaagtttc cagtctcaac attgggtcaa gaccagcagc ccgtaaaacg
    12481 attacggaaa ttacaggctt gcgtgccatt ccttgggtct tttcatggtc acaaaatcgc
    12541 atcatgttcc caggttggta tggggtaggt tcagccttta aacgttatat tgaccgagca
    12601 caaggtaacc ttgaacgcct ccaacacatg tatcagacgt ggcctttttt ccattccttg
    12661 ttatcaaatg ttgatatggt attatctaaa tcaaacatga atattgcctt tcagtatgca
    12721 caactagctg aaagtcagga tgttcgagat gttttttatg agattttgga tgagtggcaa
    12781 ttgacaaaaa atgttattct tgctatccaa gaccatgatg atctattgga ggataatcct
    12841 tcccttaagc atagcttgaa gtcacgcttg ccgtatttta atgtgttgaa ctatattcaa
    12901 attgaattga ttaaacgttg gcgtaataat caacttgacg aaaatgatga aaaattgatc
    12961 catacaacta ttaatggcat agcgactggt ttacgtaatt caggctaatg ctcttgagta
    13021 ttaattggta tttatagaag ttttcaagtg actaatcaga attttaaagg ttagcccttg
    13081 gagcttcttt ttttgaggat tcttgacttt tccttttgtt tgatatagaa agatgttata
    13141 atgctaaatg attatctcaa gaagtagagg gaaaactatg aagattgata aaaggcacct
    13201 cttaaattat tctatattat tgccttattt aattttatcg gtgataggct taataatggt
    13261 ttactcaacc acaagtgtca gcttgattca agcacatgct aatccgttta aatcggtgat
    13321 taaccaggga gttttttgga taataagtct agtggctatt acatttatct ataaattaaa
    13381 attaaatttt ttaacgaata caagggtatt aactgtggtt atgttaggag aggcctttct
    13441 tttaattatt gcccgcttct ttacaactgc tatcaaagga gcgcatggtt ggattgtgat
    13501 cggaccagtc agctttcagc ctgctgagta tttaaagatc attatggtat ggtatttggc
    13561 attgaccttt gctaagatcc aaaaaaacat tagcctttat gattatcaag cactaactcg
    13621 tcgaaaatgg tggccaacac aatggaacga tttacgggac tggcgtgttt attctttgtt
    13681 gatggtactt ttggttgctg ctcagcctga cttgggaaat gcttccatta ttgtattgac
    13741 agccattatt atgttttcta tcagtggcat tggttaccga tggttttcgg ctatcctagt
    13801 catgattacc ggtctatcca cggtctttct tgggactatt gccgttattg gggtagaaag
    13861 agttgctaag attcctgttt ttggctatgt cgctaaacgt tttagtgcct tttttaatcc
    13921 ttttcatgac ttgaccgatt caggtcacca attagccaat tcttactatg ctatgagtaa
    13981 tggtgggtgg tttggccaag ggttaggaaa ttctattgaa aagcgtggct atttgcccga
    14041 agcacagaca gactttgtct tttcagttgt cattgaagag ttaggtctaa taggagcagg
    14101 gtttattttg gcacttgttt tcttcttaat tttacgtatt atgaatgttg gtattaaagc
    14161 aaaaaatcct tttaatgcca tgatggcttt aggagtaggc ggtatgatgc tcatgcaagt
    14221 ctttgttaat attggtggta tctcaggctt aattccatct actggtgtta ccttcccttt
    14281 tttgtctcaa ggtggaaata gcttacttgt cttatctgtt gcagtgggtt ttgtactaaa
    14341 tattgatgct agcgaaaagc gagatgatat atttaaagaa gctgaattat cttatcgaaa
    14401 ggacactcga aaagaaaata gtaaagttgt aaatatcaag caatttcaat aatattctaa
    14461 aatctccttt aaggagattt tagaatatag acaatcccag tttgatgctc aaaaaacttg
    14521 aactgctctg ctgatttctt atttttgcgt gtgatatgag cccaaacagc tgcttttgct
    14581 gtggcaaaaa taaggcgtgg aattccaaag gaagtcgctg ctataagcac cacctaagga
    14641 gagtatcgaa aaagactttt tcttctattt aaaaaataaa gaaatggcaa aaaacataaa
    14701 atacttgcat tttcattgta atttgctaca atagtaaggt aaagttagac tgtatggcct
    14761 accgtctatc tataaaatat attttattgg aggcttttcc taaaatggca aaagaaaaat
    14821 acgatcgtag taaaccccac gttaacattg gtacaatcgg acacgttgac catggtaaaa
    14881 ctactttaac agctgcaatc acaactgtat tggcacgtcg cttgccttca tcagttaacc
    14941 aaccaaaaga ttacgcttct atcgatgctg ctccagaaga acgcgaacgc ggaatcacta
    15001 tcaacactgc acacgttgag tacgaaactg caactcgtca ctatgcgcac atcgacgctc
    15061 caggacacgc ggactacgtt aaaaacatga tcactggtgc cgctcaaatg gacggagcta
    15121 tccttgtagt tgcttcaact gatggaccaa tgccacaaac tcgtgagcac atccttcttt
    15181 cacgtcaggt tggtgttaaa caccttatcg tgttcatgaa caaagttgac cttgttgatg
    15241 acgaagagtt gcttgaatta gttgagatgg aaattcgtga ccttctttca gaatacgatt
    15301 tcccaggtga tgaccttcca gttatccaag gttcagctct taaagctctt gaaggcgaca
    15361 ctaaatttga agacatcatc atggaattga tggatactgt tgattcatac attccagaac
    15421 cagaacgcga cactgacaaa ccattgcttc ttccagtcga agacgtattc tcaattacag
    15481 gtcgtggtac agttgcttca ggacgtatcg accgtggtac tgttcgtgtc aacgacgaaa
    15541 tcgaaatcgt tggtatcaaa gaagaaacta aaaaagctgt tgttactggt gttgaaatgt
    15601 tccgtaaaca acttgacgaa ggtcttgcag gagacaacgt aggtatcctt cttcgtggtg
    15661 ttcaacgtga cgaaatcgaa cgtggtcaag ttattgctaa accaggttca atcaacccac
    15721 acactaaatt caaaggtgaa gtatatatcc tttctaaaga cgaaggtgga cgtcacactc
    15781 cattcttcaa caactaccgt ccacaattct acttccgtac aactgacgta acaggttcaa
    15841 tcgaacttcc agcaggtaca gaaatggtta tgcctggtga taacgtgaca atcaacgttg
    15901 agttgatcca cccaatcgcc gtagaacaag gtactacttt ctcaatccgt gaaggtggac
    15961 gtactgttgg ttcaggtatc gtttcagaaa tcgaagctta attttaatta agttcccaga
    16021 atagcaattt aagtcagaca acttaagttt gaagaagccc ccaattcggg ggctttttgc
    16081 tatcttaaac tccataaaat gctgataaat aagaatgaaa agctttcaga aaaagggtgt
    16141 ttatttgtga aaatattcct aaaatataag caattccttt taatcgtcgg tcatttgtgc
    16201 taaaatagaa gatataaata aaaaaagaag aggtatgtga tatgtcacgt aaaccaatta
    16261 ttgctggtaa ctggaaaatg aacaaaaacc ctcaagaagc aaaagcattc gttgaggctg
    16321 tagcaagcaa attaccttca actgaccttg ttgatgttgc cgtagcagcc ccagctgttg
    16381 atttggtaac cactattgaa gctgctaaag attcagttct taaagttgct gcacaaaact
    16441 gctactttga aaacacaggg gcattcactg gtgaaacatc accaaaagtg cttgctgaaa
    16501 tgggagctga ctatgttgtg attggtcact cagaacgccg tgattacttc catgaaacag
    16561 atgaagacat taataaaaaa gccaaagcaa tctttgctaa cggtttaact ccaattgttt
    16621 gttgtggtga gtctcttgaa acttatgaag ctggtaaagc cgttgagttt gtaggtgcgc
    16681 aagtttcagc agcacttgca ggtctttcag cagagcaagt agcttcactt gttttagctt
    16741 atgagccaat ctgggctatc ggtactggta aatcagctac tcaagatgat gctcaaaaca
    16801 tgtgtaaagc tgttcgtgac gtggtagcag cagactttgg gcaagaagtt gctgataaag
    16861 ttcgcgttca atacggtggt tctgtaaaac ctgaaaacgt taaagattac atggcttgtc
    16921 cagatgttga cggtgccctt gtaggtggtg catcacttga agctgatagc ttccttgctt
    16981 tgcttgattt ccttaactaa gtcatctaaa aaagaaaagg ttaaatcctt ttctttttta
    17041 gatgacttaa ctcttactat gagagaataa aggtgattct acaggtaaat gcattagcct
    17101 aatagcggtt taatagcttt tttgccaatc taatgaaatg atatttgatt ggaaatggat
    17161 aacatctaaa tgtccctggt ttttgcaaaa taaagccatt gaaattctgt ttaaagcgca
    17221 agacaccgtc tgaaccatca aactttccag taataccaag aaaattatat tgtttgatgc
    17281 ccttgtgaat tgccttgagc atagcatgct cttgtaatag agctgggcta taaaatttat
    17341 taaatttggg gtaagatcct gaaaagaggt agacggcctc ttgttcggta tagataaaaa
    17401 gacttcccgc aaggaacaca tctttagttc cgtactcttc aagaaaatgg agagcttcag
    17461 aaattcttac ctgaaaggtt tcgaattggc tggatagttc ttttaatcta ttttgctttt
    17521 tttcagaatg aggatttttg ccaaggtctc ctttaacttt gttgatgctg gttgctagtt
    17581 gtagttgtct ttgtttaagg ttattaaggt aatcttcaaa attaagagtc gcaaccataa
    17641 attcacaaga gtctccaaaa ctatcataaa agtcctgata gtaagataat gatttgtcaa
    17701 ggtaatctct acgatcagag gttgcttcag taatttcttt gaaatggtgt aactcatctc
    17761 gtttcagttg acgtaattta ataccaaaag tattagcttt ttttattaaa gcttttcctt
    17821 ttttgctgaa ggaatgggtt aaacgtgagg aatcaattcc ttctaacttt ttaacgtaat
    17881 gccaaacagg ttcaccctca ggatagcctg tttttaaacc gtcatgttta tatcctaacg
    17941 ccgttaaaag gctaataagg tcagtgtttg gatgagaaat agggatgcca tcagtatcaa
    18001 atgactgata attgtcgtaa ggttttaaaa tgagttcgat aacgtctttt tgtttgacat
    18061 agtctttaag ttgagtgtaa aaatgttcta attcttcggg atgattggta ttagggcctg
    18121 cattgagttc catacgccaa cctccagcca ctttttggct aaagaccata gcagcaacct
    18181 ttagttcacc atctttttca agtccaagaa attgtggttt tgcccctctt ttagccatca
    18241 agctggccat ttctgaggtt tggataaagg aatgatggac gagactgtgg cagtaatggt
    18301 caaattgttc ttgtgaaatt tcaattagtg ccattagttg tgctccttaa tttttttctt
    18361 aagttatagg tcaagatggc taagcttgaa acaaggccga caggaatgtt gaattcacct
    18421 gcaaattctt cgatggtggg gttgagtcta gctttgaaat gatagagtcc accatcttgt
    18481 tgattttcca cacctcctaa attatgccag cggcagcctc ttttaaaagc ttctttagcc
    18541 gtttcatacc aggttaaaag aggagcttga taattgcgat aatcatcgtc cattcccgca
    18601 taaagatttt cagaagtctc gccataaatc aaggttaggg tggcggctag tgggatgcgg
    18661 gttttgcctg cagcgagctg ctcgcttaaa atggttaaat ctttttgtag ccgtgcaatg
    18721 gttttttgat tttctgctac tttgctaggt ttactcttat ctgttaatcg tgcttgttct
    18781 gctaaggcct tgtccaactg ttggatcaag agtttttttt gttcaggtaa gtctaaacta
    18841 gccatagtaa tgtaggactg accagcataa atatctagta atttttgata gtaggattta
    18901 cctcgaagga taatgccttt tcgattctcg gtttttttca tcaaggctga aaagtcgtct
    18961 agcaattcac tgccaccaat cgtgacatta acacctttat tggtagcggt acgaatggat
    19021 tgcttggctt ttttgggcag gctgtcaagg tcaaaatcct tggcgtagat gttggcttgt
    19081 attcttggtt gaatagtgtc ttcgagttct tttgttctgc cagaccattc gacaccaagc
    19141 ttttttagaa aagcaataat agagagtgtc acatcatttt ctttgctctc ttttccttct
    19201 aaagtttgtt tgatgactaa acttggatca attttgacaa aaagcgctcg tttgcttttt
    19261 ccgaaagttt tgagcgtttt aataacgaaa tcaaggagtt caaaattggc gtagtccatg
    19321 attggcccac gtgggatata aatcatagtg aaaccaagag gaagttttct gattaaacaa
    19381 gcagcagcag caacttgtac cccattttca taaaagctga tgcgttcgtg tttccagtta
    19441 tctttgactt ttccccactt tgaactttga agtaagccag cttgtggctg tgctaagaca
    19501 aactgatcat gttcttcagg agatattccg attttatatg tatacattag tttaataccc
    19561 acttgcgaat agcatgtgcc acaccactgt cattgttgga tttggtgatg tatttggcta
    19621 tttttttcaa ttcaggaacc ccattttcca tgacaacagg gttggcaacg acttctaaca
    19681 tagcacgatc attttcagca tccccgatgg ccatggtttg tgacatatct agcccgagtt
    19741 ttttagctaa gtgtttgatg gcattaccct tgctgacggt ttttggcatg aactctaaat
    19801 aaaaaggggt tgacttgacg atggtgtact tatcaaagaa atgttgagga atttgcttga
    19861 tggccgcgtc caaaaggtca ggttcatcaa tcatcatcat tttgatgatt tctttattgg
    19921 tcatttcttc gggagtgcga tagaaaatag gcatgtttac caaagtcgat tcatgaactg
    19981 tgtattttcc aatattccga ttggcagtat aaataccttc tttggtaatg gcatgcatgt
    20041 gaattcctaa ttttcggctc aaaaattccg tttccagata atcgtcgtaa gtcatcaatt
    20101 ctttaacaat ttcttcgccg gtctcggcat cttgcaccaa gccaccatta aaagtgataa
    20161 cgtggtttcc tttgtggttg agttctaatt gttcgagtaa tgaaatcact ccggcaatag
    20221 gtctgccagt agcaatcacc acatgaaccc cttgggcttt agcctcttgg acagcttgaa
    20281 aaacatcatc tgtaattcga cggtcatcag tgagtaatgt gccatcaata tcgacagcaa
    20341 ccagtttgat agacatggca tcctccttgt ttttttgtgg tttatcttaa aaatgttctc
    20401 cctcctatct tactaaaaat aggtgctttt gtcatgggat gggaaaggaa acttaaggaa
    20461 aaacgagaga aaacgtttta gtttagctca atcgcctctg tagtaactaa aaagattgaa
    20521 gaaagtactc tttcttcaat cttagaatgg agacaaaccc agtttgatgc tcaaaaagca
    20581 tgaactgctc tgctgatttg acaactgatt tcttatttct gcgtgtgacg tgagccaaaa
    20641 cagctgcttt ttgctgtggc aaaagtggtg tcaatactgg cagtgacgtt acgcggggtt
    20701 tttgagaccc taggctcaaa aataaagcat agaattccaa aggaagtcgc tgccataagc
    20761 atcacctaag aaaagtatca aaaaagactt tttctgcaat cttaaagggc tttactgact
    20821 aaagtgaaaa tgcccattgg aaatataact cataaaagtt tccttgctag gagcaaagag
    20881 gtcatcgagc tctaacattt cctttggaaa ataaaagcga cggtctccgt aggttgtccc
    20941 tgttaatgct ttgacaatag gagataactg agataattca gcaaggctcc cgtctttttg
    21001 tatcatttca atctgagttc ttggattttc gagttctggt cgataaatat cataaggcag
    21061 gtcgaagttg atatggatac cggtgtaata atctggatca aagccaactg attcgaccaa
    21121 ttggcgcaag cgttctaact ctccttggga atcttggtca aaggtaacag acttcagaat
    21181 ttttcgatta ataaagcgac tggccaaatc tgataagata tggtcttcgc ttgccatcca
    21241 gacttggaag taagtattca tgactccgtc atctagggct atataatctg ctaaattagc
    21301 cttcttttca aagaagggga tgagtccagg agctgttttt tggaaataag cctgttgctt
    21361 aggatataag tgttgggctc ttttgaggag attttgtaag atgagctcaa cagcacggct
    21421 ggcgggatga aaatagacct gcatgtacat ttgaaaacga ctgacaatat agtcctcgac
    21481 agcatgcata ccactatgtt caaagacaat gccatcttca acaggtcgaa tcacacgtag
    21541 aatgcgcatt aaatcaaact gaccataatt ggcagcagaa aaataagaat ctcttaaaag
    21601 gtaatccata cggtcgcagt caatctggct ggaaatcagt tgcacaactt gcttgttagg
    21661 ataagtatga ttaatcacgc tggcaacttt gtctggaaaa tcaggagcat gacgcaccaa
    21721 aatggcattg atttctgtct caggattggt aattatctcc tgagtaaagg cctcgtgatc
    21781 cgtatggaaa agggcctcaa aggtatgaga ataggctcca tgaccaatgt catgtaagag
    21841 ggcagccgtc atagtgacca gactttcatc tttattccag atatcagcgt atttctcttc
    21901 aaaaatagct gtcacccgac gggcaatttc gtaaacacct aggcagtgag aaaaacggct
    21961 atgttctgcg ccgtggaagg tgaaagctgt tgtagggact tgtttgatgc gtcgtaagcg
    22021 ttggaattct ttggtattga taaggtcata aattaatgga ttatcaatgt ggatatagtt
    22081 gtgaacaggg tcacgaaata ctttttcatt cataattttt attatatcaa aaattgataa
    22141 aataaggaga aattgataga ataaacagac gagcttagaa tagaaagaag agttatgaaa
    22201 ttacagttaa ccaaccatat tcgctttgga gatgagacag agattattca agaaattcat
    22261 gattgtgagt ggcgagaaaa agggggctat cagtacctca tctatcaaaa cactgacaag
    22321 gaaaaggttg tcatcaagta taatgagaca gaactgacca tgagccgttt ttccaaccct
    22381 cagtcaatca tgaaattttt tgcaggcaag aaggtcctga ttgctctacc tacaccgatg
    22441 ggggttcaac aatttttgac agatacaagt cattaccact tagattgttc ttgtcaaaaa
    22501 ttggaccttc attatcattt gcttcaagct caaacagaaa tgctatttgc aagctatcat
    22561 cttgagctga gttgggatta actaaaaaga tagcagtaaa tcatctgatt agggattatt
    22621 cttgtttttt agtcaggttt ttactataat ggttattata tcaagggagt agcagacggc
    22681 tagccgtgat tgtatcgtca atacggaatt tcggtttccg gtacaatctt aaacagcgag
    22741 acttgtttaa gaaaataaac aggtcttttt atttgaacaa ctaaaataaa aagaccaagg
    22801 aggtactaat ttgtctaaag aacaaagaca tgaagcattt tacacgcaaa gtgaagagac
    22861 cgtactggct caattagaga ccagtcgcga aggtcttacg tctgcccaag caaaagagcg
    22921 cttagcagaa tatggtcgta atgagcttga tgaaggcgaa aaacgaagtt tatttatgaa
    22981 attccttgat caatttaagg atttgatgat tattattttg attgtggcag cccttctttc
    23041 agttcttaca gaggggatgg aaggtttgac agacgccatt attatcttgg ctgttgttat
    23101 cctaaatgct gcttttggtg tttaccaaga aggacaagct gaagctgcta ttgaagcctt
    23161 gaaatcaatg tcaagtccac tggctcgtat tcgccgtgat gggcatgtga ctgagataga
    23221 ctctaaagaa ttggtccctg gagatattgt cttgctagaa gcgggagatg ttgtccctgc
    23281 tgatctgcgt cttttggaag ctaactcctt aaaaatcgaa gaagcagcgc ttactggaga
    23341 atctgtaccg gttgaaaaag acttgtcaac ggctgtttca gaagatgccg gtattggtga
    23401 tcgcgtcaat atgggatatc aaaactcaaa tgtgacatat ggtcgtggga ttggggttat
    23461 cactaacact ggtatgtaca ccgaggttgg gcatattgct ggtatgcttg ctaatgctga
    23521 tgagacagat acaccattga agcaaaacct tgacaacctt tctaagattt tgacttatgc
    23581 gatccttgtg attgcagcgg tgacttttgc tgttggagtc ttcttgcgtg gtcaacaccc
    23641 gcttgaaggc ttgatgactt cagtagcgct tgctgttgca gctattccag agggacttcc
    23701 tgcgattgtt actgttgtgc tttctcttgg aactcaagta cttgctaaac gtaacgccat
    23761 tatccgtaaa ttaccagctg ttgaaacgct aggatcaact gaaatcattg cttctgataa
    23821 aacgggtacc ttgacaatga atcaaatgac cgttgaaaaa gtttatacca acgggacact
    23881 ccaaagttca tcagctgata ttgcttttga caatacgaca cttcgtgtaa tgaactttgc
    23941 taatgatact aaagttgatc cttcagggaa gttaattggt gatccaacag aaacagcttt
    24001 ggtacagttt gggttagacc acaattttga cgtacgtgag gctatggtgg cagaacctcg
    24061 tgtggcagaa ctgccatttg attctgaccg taagttgatg tcaaccattc ataaacaagc
    24121 tgatggcaaa tatttcatcg ctgttaaagg tgcaccagat cagttattga aacgtgtcac
    24181 acagattgaa gaaaatggac aaattcgtcc tattacggat gctgataaaa aaaccattct
    24241 tgataccaat aagagtcttg ccaaacaagc tcttcgtgtc ttgatgatgg cttacaaata
    24301 ttctgatgct cttccaacac tggaaacaga aattgttgag gctaatcttg tcttctctgg
    24361 tttggtaggt atgattgacc ctgaacgtcc tgaagcagca caggctgtta aagtagctca
    24421 ggaagctggc attcgtccaa tcatgattac tggtgaccac caagatacag caaaagctat
    24481 tgccaaacgt cttggtatta tcgaagaaga tggcgttgat catgtcttta caggtgctga
    24541 actcaacgaa ttatcagatg aagagttcca aaaagtcttt aaacaatact ctgtgtatgc
    24601 ccgtgtatct cctgagcata aagttcgtat cgttaaggct tggcaaaatg aaggcaaagt
    24661 tgttgccatg actggtgatg gggttaacga tgcaccatct cttaaaacgg cagatattgg
    24721 tattggtatg ggaattacag gtacagaggt ttctaaggga gcttctgaca tggttcttgc
    24781 cgatgataac tttgcaacca ttatcgttgc ggttgaagaa ggacgtaagg tcttctcaaa
    24841 tattcaaaaa acaattcaat atttactttc tgctaacatg gcagaagtct ttaccatctt
    24901 ccttgcaacc ttgtttggtt gggatgtgct tcaaccagtt cacttgcttt ggattaactt
    24961 ggtaactgat accctaccag ctattgctct tggtgttgaa cctgctgagc ctggtgtgat
    25021 gaagcacaaa cctcgtggac gtaagtcaag cttctttgat ggtggggtga aggaagccat
    25081 tctttaccaa ggtgccttcc aaaccatttt ggtgcttggg gtttatggtt ttgccttgat
    25141 gttcccagaa cacacaagct atcatgatgt ccatgcagat gctttgacca tggcttatgt
    25201 gaccctcggt ttgattcagt tggtacatgc ctacaacgtg aaatctgttt accaatccat
    25261 ctttactgtt ggattgttca aaaacaaatt gttcaactat tctattccag tggcctttgt
    25321 agcattaatg gcaacagttg ttgttcctgg atttaatcaa ttctttcatg tgacacactt
    25381 gacgatcaca caatggttag tggttattat cggaagtctc ttgatggttg ttcttgttga
    25441 attggtgaaa gctgttcaac gatcacttgg tcaagatgaa aaagcaattt aatagacgct
    25501 aaaaggtttg agacaattgt ctcaaacctt tttttataat cgttacaacg ataaggacta
    25561 ttggtatgcc tgacattaaa aagttgattc ttgccaattg acttgacagt caaaaatttg
    25621 ttgtttggct tcttgactgt gaccttcaat atcatcgatt aaaatcttag tagcctgttc
    25681 gccttcttca taggcgggtt ggataatagt tgttagtgtt ggtgaagaaa attttgtcca
    25741 ctcaatgtta tcaaatccta ctagtccaat ctctggtata ttaaatttaa gagacttcat
    25801 agctgtaaat acttttggaa gagcccaaca attggggaca aaaaccaaac tctttttagt
    25861 cagtgatcct tgcaaaaact gagcaattgc ctctgaagaa gtctgatttt catcaataat
    25921 catttcttga tgagtaagat gattagcttc taacacatca atgaagcccg aagcgcgttc
    25981 aatacgagtg ctaagtaaat tggggttacc cgtgatcatg ataaaatgtt cataaccttt
    26041 atcgatacat tgttgaatgg tatcataaac agcatcataa ttattagttt tgacccagtt
    26101 tgtgcgatgt tcgtaaagtt ggctatcaaa aaagaccact tttttcttct taatatcgat
    26161 aatgcgagaa tacttcctga aattagaggt aggctgaatg ataaagccat ccactccgag
    26221 gttcagcatc ttttcaatga gttcatcttc gcgagaaggg tcgtagttgc tgtttccgat
    26281 gataatttgg taaccaaatt cctgggcttt gctttcaatt cccttaacaa tttgattgga
    26341 aaaactattg gtaatatcac caataaccac gccaatgagt ttagtagatt tggcgtttag
    26401 acttctggca gcaatgcttg gtttataatt ggtagcttca atcgtgcttt taatgcggtt
    26461 tttagtttct tcagacatct tgtcaaacct accatttaag tagaaagaaa ctgttgtttt
    26521 tgaagttttc gctaattcag caatatcttt gatggttact ttgcgctgca ttgcatacac
    26581 tcctttaaaa tagtaatggt tagtcatatt ataacataag gtctcgtaaa agatttagtg
    26641 tttcaatcga ataatctcat tcgtgatatg gttgataaca accacctttc ctctgatttt
    26701 atgaccattt aggagaacta gtttttctcc tttaattaaa tcctcttgga gaacacagat
    26761 actgtagtca aactgctttc cttttaaatg ccaggaaagg gcagtctcaa ttgggttgcg
    26821 tttgcctgtt tgaagtggtg ttaagggagt aactttggta tcgtcaggga ctaatagggt
    26881 actggtgcaa cccttgtctt taaagggttt tcgtttgatg agtttgtgac tagtcaggta
    26941 ttggttgtat ttttttgaca aaatcgtttc ttcgatagtg aaagggcagt cactgattag
    27001 tctgaggtca ctaaggtgat ctgtctttac ttctaagtga ttatcaagaa taaattgact
    27061 gacaagacag tggttacctt gagcctgaat cgtatcaatg ataagcaaaa ttcctggtgg
    27121 taagatgaga atttgtcggt catgttggta gttgttccta tcagagaatt gcgtctggta
    27181 gctaccatca ataaagtaaa tgtgtccatt ttgtcgaaga tgacaataat tggatttggg
    27241 gtaagaaagg taagcccagg agctatcaat ctgctcaggt aattgttctt ctaagaaggc
    27301 ggtagaatga tggctagcgc tttttagggc atagcgtaat ggttcttctt tgtaagtgta
    27361 tcgcccagca tcacagaaaa gtggttgtcc tttataatag agacagagac tattttggtc
    27421 gctatgggta tgggaactac cgatgggccc atttttgaaa aaaagataat aattttcttg
    27481 attgatggta atgtgcccgg aatgttcaaa gtgatgcgca aaagttggta aggtttggac
    27541 gggtaactgt tcaaaagtgt ggacaccttt ttctcctaga aggagaagac tatcgatatc
    27601 aagtgtggga aaagcagcag cttttaagtg tggttcttca aggatagtgg ctgctagtgt
    27661 tagaatatct ctggtatcgg taaagtcact gtctcctaag gggatctgtt tgtggtcagg
    27721 gccagtcatt ttgaggagat aatcactcat ggcaagcaaa gtaggtctaa gctgtggaag
    27781 ataatcaggg actagagcag ttaattccag aagggctttg agtacttcca catgatacat
    27841 ggttgattgt tcaaattgac tgccgtcttc gagaatttgg agtgcgattt gttgcgtcaa
    27901 ttcttttcta gcgaaagctg ttgctgcagc aatatcaagg tcagaaccat agtaggcatc
    27961 agctaataaa atcgcagtgg tttgcaagat tccccaattg ctgaggctgt atttatcaag
    28021 gtagttggca tggagaaatt gaagttgttt ttccagggaa gctaggatga ggctttcctc
    28081 ttgcttggtt aaggcgttaa aaagattaag gtaaatcaaa catttgaccc aagcaaaaca
    28141 acggatgcca gtatctaagg ttctagtagc taacccctta gggtctaaag gaatagctga
    28201 ttcaatccaa ttgaggatga aacttttggc ttttaggaga taccgttcat ctctttcaac
    28261 aaggtagacg aggattaatt tttggagata ggtctgtcga tttaacataa aattccactc
    28321 aggatcatct gtgacagcct cttgccaagt aatgggatct aagtggtaag ggatgtggca
    28381 gggctccata tcccaattat cttcaaataa aaaggtattg gttaagagta ggtcagctat
    28441 tcttttttga tcagcatagc tgtcagtctg atagtctagc aggtaattcc tgcaaaagtc
    28501 tggattgact gtttctttaa agcgtgtgaa ggcaagagac ataggacatt tcctttccaa
    28561 aagaaaagag acctcatcat gtgcttagga actatttccc tatgctcata acaggtctct
    28621 gtgaaccatt aagctaaaat acctaaccag ctaccgataa taccgataag aacagtgagg
    28681 agaaccaatt tgtaggttgt ccattttttg tttttaataa ggtaatacat gagtaaggta
    28741 aagagagctg gtaaaagtgc aggagcaatt ttatcaagca ttccttgaat agtcacaaat
    28801 ttttgtgctg tattggccgc atccaccttg ccagccgcaa aagtaatggg aacagttatt
    28861 ttaacagaag tagcagctaa tccagcaata acggtaacac cgacaatatt tgccatgcgc
    28921 gacacaaccg ccatttgttc acttaattta tcaataaagc ttgtgccgac tttatagcca
    28981 tagagaccag aaccgatttt aatagcagtt aggataatat tcatggctag gaagaagaaa
    29041 attggaccta aaactaaacc atcactagct aaggaagctg cgatcgttga aaagagtggc
    29101 gctaagcaaa attgtgataa ggagtcaccg ataccagcta aaggccccat aagtgccatt
    29161 ttgatgtttc gggtttcttc ttcaggacga tcattttcta acatgaccaa gtgcagactg
    29221 gtgatgaaag gaaggaagtg agggttagta ttgtaaaact cacagttatc ttctaaagct
    29281 tgatataagc cctttttatc actgccaaaa tgttttttca aggcaggata gatgacattg
    29341 gcgtatccga taccttggta attactgtaa ttaaacccat tttgtaggaa gaaagctctc
    29401 agagctgttt tgaggtaatc tttcttggtt aatttattag atccagtcat cttgttggcc
    29461 tccttggaca gttgttgcat cgacttgttt aggtttgttg taaaattcat tcaaggcgaa
    29521 aatgatacca ataatggcga taccgatggt tggaatttgg aggtaggctg cacaaacata
    29581 accaatcagt acaaaaggaa tcaactcctt cttggccata actgataaga tcatggcaaa
    29641 tccgatagca ggtaacattt taccagcaac agtcaatcca ttgagcaaga caggtgggat
    29701 ataatccact aagtgaagga gagtatccat tgacaaggca cctaataaac caagtcctaa
    29761 tccaaggata gcaaaagacc aaatggtccc attagcagcg aatttgaagc ctcttatatt
    29821 gcctttttgc aattgctttt tagcggtttc aggagcccca gcaaaagcgg tataggcgaa
    29881 tgtctgaaga aattggatgg caactgcgat tggtgttgat aaggctaggg ccgcttctgg
    29941 ggtgacttta ccagcactgg taatagccat caaggtacca aagataccag ggccgatagg
    30001 attaggagga acggtaccac cggcacccac tccaaatccc atataagcta attcggagat
    30061 agctcccatg ctaagtgcag ttggcaagtc acctaagatc atccctacac caaaagacag
    30121 aataatacaa cggttggtgt agatgccaag taacattccg ctaaaacaaa aggctgtcca
    30181 aagaccaatt aaaagtgcct gtaacaaatt gatatccata tcaatctcct ttattcatta
    30241 aatataatct aagatgtcaa cgtcagaggc gctattgcca gctggtgttg ttttggtgtt
    30301 aaaaaccact tggtggtcat gggctaagca acgaatagca gacttgtctg tttctcccag
    30361 cgaaataaac tgggtaatcg caaccttatc atcagtttta tgaatattac cgatattgat
    30421 ttctgtgatg ggaacacccc cttcaactag gcgtttggca tcttgcaaat ctttgaccac
    30481 gatgaagata ctttgagcag gacttgcttt atgaatgatg tcaatcactt tttgaatgga
    30541 gaaaaaacga atggcaatac tggatgggat aactgttttc atgagggatt gttgaatctt
    30601 atcttcagaa accgcatcgt tagcgacgat aacagtgttg cagttgagga atttgaccca
    30661 taattgcccc tgtccgtgaa tcaagcgctc atcaactcgc gtcatgataa tgtttggttg
    30721 tgtcataaaa tctccttttt aaatgtgatt atcggtgttt gttaccagta agggttccag
    30781 tctttgtaga aacgaagcag agcttcaagg tagtaatagt ctccccagat atttccttca
    30841 tccacccctt ttccagagtg ccaagaatag actccgtgaa gcagtagagg agcaccagga
    30901 ttgagatctt tgttagcata atccttaatc aaggctctta gcatgctgtg catggcagct
    30961 tcatatgttt ttttatcagg atcatgatct ggtaaggttt ttaacatttc atgaatacca
    31021 cacacggcaa tggctgttgc tgaactatca cgagactgct cgctaccatc tccaaaaatt
    31081 aaatcccagt aggaaacctg gtctttagga agacggttca agaaataatg ggtcatgccc
    31141 ttgaagagtt gaatgagttc aggttctttt aggaaacgat aggtcaaagg aataccataa
    31201 atgccccaag cttgccctct ggcccaagca gaatcatcgc tataaccttg tcgggtgacc
    31261 cctttaacag gatcgcctgt ttctggatca aagtagaagg tgtggtaagc agaggcgtca
    31321 tctcggatga catgattggc tgaggcgtag aaatggttga tcgccatgtc acggtaacgg
    31381 ttgtctccag tttcttggct agcaaaaaag agtaattgga tgtttaggag acaatcaatg
    31441 attaaacggt aatcttcttt tttccccagt tctccccagg cctgaataaa gccgcctttg
    31501 tcttgatagc gttggactaa tttgtcggct gctttcaaag cagcttctct agcctcaggt
    31561 gtttttaaaa gtttccactc agccatgcaa gaaggggtgt aaagaaagcc caaatcatgg
    31621 tgatctagct caatgtctct tgttactcga tctaggaaag aaaggtcatt tgcttgagcc
    31681 agagctttaa tagcatcatc cccactgtat tcgtaagcta accaaaggca acctgtccaa
    31741 aaggcattgg tccattctgt attatccata atggcatatt ggttatcttt ggtagctggc
    31801 gttggaaagt cttctttaaa atagtccata ttaagccgaa cttgtttgag ggctaagtca
    31861 agggcctgag taatgtcttc ttggcttagg aaatcttctt tggtaaatcg ttcaggctgc
    31921 ttgataggtt caagagcaat ggtttttaaa ggtcgtgtca ttaaatgcct cctaactcat
    31981 cgtctgtcgc accgtcttct tgctgagaaa gagttttcca gtcaacaatg ccttctttag
    32041 aacatgttat aagtcctgat actaagtcgt caaaagaagc tgctgtttgt cttgcaaaac
    32101 tagcttcaat cagcatggct aagttaaggc cagaaagaac attacacgtc gtgtttggaa
    32161 gagattccat taaggttgca gcgactttaa aaggggtacc tcctagaagg tcacaaagaa
    32221 ctaaggtttc ctcttctggg attaaagcac gagataattg gtcctgcaca tcagcggcgg
    32281 tcatctcagc agtaaaatca attgcagtta ctttttcttg ttgtccggca attaactcta
    32341 gggatgagac aatcccactt gcaaagtgtc catgtcccat aacaataata gcaatcatga
    32401 taaaactcct tttggtttac cggtttactc aaaaatattg tagcgctttc aaaatttttt
    32461 gtcaatactt ttttaaaaag atttttgaaa aagaaaaagt gaacggttgt gctaaaaaag
    32521 aatcaaaaat ggtctttttt gtcactataa tgtagaaaag tcttaatagc tattaagtat
    32581 aggagaagac taaacaagaa tgacagtttg gataagagca ctcttttaaa ttttgaaccg
    32641 ttttcataaa catcttgaca aaattagttt actttgttat agtcttggta aacaggttta
    32701 cttaatttaa taacaaaaag gagaatccta tggaaaatat gttttcgtta caaggtaaga
    32761 ttgccttgat taccggagca tcctacggta ttggttttga aattgccaaa gcctatgctc
    32821 aagcaggtgc aaccattgtg tttaatgaca tcaaacaaga gttagttgat aaggggttgg
    32881 ctgcctatcg agagttaggg atagaagcgc atggctatgt ttgtgacgtg actgatgaag
    32941 ctggtatcca acagatggtt agtcaaattg aagatgaagt aggtgttatt gatattctgg
    33001 tcaacaatgc aggtattatt aggcgtacgc cgatgcttga aatggccgca gaagattttc
    33061 gtcaagtcat tgatattgat ttaaatgctc cttttattgt gtcaaaagca gttttaccat
    33121 caatgattgc aaaaggacat ggtaaaatta taaatatctg ttctatgatg agtgaattgg
    33181 gccgtgaaac agttagtgcc tacgcggcgg ctaaaggtgg cttgaaaatg ttaaccaaaa
    33241 atatagcctc tgagtttgga gaagccaaca ttcaatgtaa tgggattggt cctggctaca
    33301 ttgcgacgcc ccaaacagct ccgttgagag agcgacaagc agatggtagt cgtcatcctt
    33361 ttgatcaatt tatcattgct aaaacaccag cagcacgttg ggggacaaca gaagacttag
    33421 ctggtccagc tgtttttcta gctagcgatg ccagtaattt tgtcaacggc catattcttt
    33481 atgtcgacgg tggcatttta gcttatattg gaaaacaacc ttaatcacac actagaaaga
    33541 gattttatat gaaaattgca ttaattaatg aaaatagcca agcggctaaa aatggtatta
    33601 tttacgatgc ccttacctca gtgacagata aacacggcta ccaagtcttt aactatggca
    33661 tgtatggcac agaaggtgaa agccagttga cctatgttca aaatggactc ttagcttcta
    33721 ttttattgac cactaaggcg gctgattttg tggtgactgg ttgtgggaca ggtgttggag
    33781 ctatgttggc cttgaacagc ttcccaggtg ttacctgtgg ttttgcgagt gagccaacag
    33841 aagcttatct cttttctcaa atcaatggtg gtaatgcctt gtccattcct tttgccaaag
    33901 gttttggttg gggagcagag ttgaatttga ccttaatatt tgaacgcctc tttgcagaac
    33961 caatgggtgg cggttaccct aaagagcgag ctattcctga acagcggaat gcacgtattt
    34021 taagtgattt gaaaaaaatt acttacagag atttgttggc aattgtcaaa gacatcgacc
    34081 aagattttct caaagaaacg atttctggcg ctcattttaa agagtacttc tttgccaatg
    34141 cggagccgtc agaattagtc gcttatttga aatcagtact tgaacagtaa ttccattttg
    34201 acgaggaaag gaggtcacat gtctaagtta ttattggttg gtgaaccgct gattcgcgtt
    34261 agccccaatc agtttcagcc tttgaccaat gcctgcgagg ctcagctttt tttcggcggt
    34321 tcagaagtta atattgcaag aactctaggc ggttttggct tagaagcaag gctatttacg
    34381 gccctaccag ataatcctgt tggtcacgct tttcatcagt ttttgaagca aagcggcgtg
    34441 gatatgtcct tgactgcttg gcaaggtcat cgcgttgggc tatattacct tgaaaatggc
    34501 tttggctgtc gggctagtca ggtttattat gaccgctgtg gctctagctt ttcggcctta
    34561 gataaggata gtcttgattt agctgccatt tttgaaggca ttagtcactt tcattttagt
    34621 gggatcagtt tggcgctggg caaaaaaact caggatttga ttgaaatact tgcgagagaa
    34681 gctaagaaaa gggatatttg tatttccttt gaccttaact ttagatctag tatgattgcc
    34741 gtagctgatg ctaaacgact cttttctcac tttgctcaat acgcggatat tatttttggc
    34801 atggaacctt tgctgttaga cagtgacgat tttgacatgt ttgatagaaa aaaggcagat
    34861 gctacgatga ttagagaacg tctggcagga ctttatcagc gctatcagtt acaagctatt
    34921 taccatacgg agcgttcaaa tgatgcccaa ggcagtaatc gtttcaaggc ttatgcctat
    34981 gatgggcagt tttacgaatc ctgtgaagtg acgaccccag tcttgcaacg ggttggaagt
    35041 ggagatgctt ttgtggcggg tttactttat cagttgttag aaggtaacga aaagcagcgc
    35101 aaccttgatt ttgctgtggc aacagctagc ctaaaatgta cagtagctga agatcagctc
    35161 tatcacagcg tccaacaagt agaagcagtc ttagctaatc aacgagatgt ccagaggtga
    35221 tgatatggga aaaatagaaa tcttaaccaa acttaaggct aatcggcttg tcctggttgt
    35281 acgaggagag tctagtgagg aagctttagc ttgcagcctg gccagtattg aaggtggcat
    35341 taagacaatt gaagtcacct atactaaccc ctttgctagt gaggttatcg gtcaattagc
    35401 tgaacgcttc aaagaagatc ctgaggtatt gataggtgct gggacagttt tggatgatgt
    35461 cactgcccgc caagctattt tagcaggagc acagtttatt gttggtccta atttcaatcg
    35521 agcagtggct ttgatttgtc atcgttatag cataccttat ttaccaggtt gtatgacggt
    35581 taatgaagtg gttacagcat tagaatcagg ggttgatatg gtcaaaattt ttcctggttc
    35641 tactgtaggg atatccttta ttcgggcaat caaatctcct ttgccacaag tagaggttat
    35701 ggttacaggc ggagtttctt cagataatct caaagactgg ctagctgctg gagtagatgt
    35761 tctaggcata ggaggcgagt ttaatcagtt agcgagccaa aaacaatata acctaatcac
    35821 taagaaagcc gctcattata tcaaaagttt gcgacaataa tctaaaaaac gtttttacct
    35881 tggctttagt gctagtaaaa acgttttttt atcattcatt caattaatat gcttctgctt
    35941 atgtttttcc agatagtata gatataagag cagctaattt aacaagatga gcctgtcgtt
    36001 tgatatatag gatataaact ttttatgatc aaacaaaagt aatacctttt gaattcgtta
    36061 gtattgacct gatctaaagt gatatcactt agaatggact agctgtacag cttttacagc
    36121 aacttatgct aaaatagaaa aaaaggagaa atgttgatga ttaaaggaat tatttttgat
    36181 atggatggtg ttttatttga tacagaacct ttttatctga ggcgacgaga agattttttt
    36241 aagacaaagg gaattcccat tgatcacttg aactctaaag attttattgg gggcaatctc
    36301 caagaattat ggaaagagtt gttaggtaaa aatagggatg atgctatcgt taaggcaatt
    36361 acaactgact atgacgctta caaacaagcg cataagcctc cttatcaaaa actgttgatt
    36421 acagaagtga actcttgtct tgaacagttg aaaaaacaag gtatcaaact ggctgtggca
    36481 tcaaactcga agcgtcagga tgttttgttg gcgttggaga caacgcaaat aaaagattat
    36541 tttgaaataa tcctagcgcg tgaagatgtt tctagaggca aaccttatcc agatatttat
    36601 aataaagcag tacaaaaact aggattacaa aaaaaacaac tgcttgtagt agaagacagc
    36661 caaaaaggca ttgctgccgc caaagcagcc aatctgacag tttttgccat taccgactac
    36721 cgatatggca ttgatcagag tcaagctgat cacaagatag atcatttagg acaactgtgt
    36781 gtaaaaatcg gttgttttgg atcgtgaagt aacgcaacta actgatcatt gatgatactt
    36841 caagacactt aagaatacgt ctaggacttt ctacagacca ataacaagaa tacgtttatg
    36901 tgtttttact gttttttagc tacctattta caatatgaca ctgaaaagag tactatgttt
    36961 aagttattat caaaaaaacc gtcgcataag atcacctaga aaagaattat gatttttgtg
    37021 atttggatgc gatcgttagt cgttgaccat ctttatgtgg caggagatat gtttaaccgt
    37081 ggtcattatt tggatttgtt tttagattag ctagtgagta aatatagaag gatcataata
    37141 ctgctttgac aaaggcagta tctgagtcca tgagttgcac ggataatgtg actcggtttt
    37201 ggttaggtat aataatattt ggaagtgtta tgctgcttgt cttcgtaggt ccattgatta
    37261 tagtcagact tactttagag ctgagctgac tgtctagaca agacgtgatg gagatgtgat
    37321 ttctgaccaa gtagaggtaa ccagaaaagg gacttttacg atgtaattga atttacttgt
    37381 tgcattcttg tgcatgttaa gagagaaaac ccagataaat caggaagtaa aatggttatg
    37441 tttgattaga attgtccttc tttttccttg aagtcttaac ttattttgat tatgatagta
    37501 gtatgactat taaggcagaa attaaggcgc tcgccaaaga aattggtatc tctaaaattg
    37561 gttttacgac ggcagataac tttgattatt tggaaaagtc attgcgtgct tctgtggaag
    37621 aggggcgcaa ttcaggattt gaacataaag tcatagagga tcgtatctcc cctgaaaggc
    37681 ttttagaatc ggctaaaacg attatttcaa ttggcgttgc ttatcctcac aaacttcctc
    37741 agcaacctca aaaaacactt tataaacgtg gcaaaattac acctagttcg tgggggcttg
    37801 attatcatta tgtggtcggc gaaaagttag acagactatc aaaaggcatt gaagagctgt
    37861 gtcgagattt tcctttgcaa caaaaagcaa tggtcgatac tggagcttta gttgatactg
    37921 cagttgccca aagagcgggt attggtttta taggaaaaaa tggtttagtt atttctaaag
    37981 aatatggctc ttatatgttt ttgggagaat taattaccaa tttagagatc gagccagaca
    38041 agcctgtcga ttatgattgt ggtgactgtc gtcgttgtct agatgcctgt ccaacttctt
    38101 gtttaattgg tgatggctca atgaatgcca agcgttgtct atcatttcaa acccaagata
    38161 agggaatgat ggacatagag tttcgaaaaa agattaaaac agttatctat ggttgtgata
    38221 tttgccaaat ttgctgtcct tataacaagg gtataaacaa ttcaccagct acagagattg
    38281 atcctgagtt ggctcaacca gaattaatac catttttgag tttatcaaat ggacaattta
    38341 aagaaaaatt tggtatgatt gctggaagtt ggcgaggtaa aaatatttta cagcgtaatg
    38401 ccattattgc gctagcaaat gctcatgata aaaccgcagt ggtcaaatta atagagatta
    38461 ttgataaaaa taataaccct attcatactg caacagctat atgggcattg ggagaaattg
    38521 tcaaaaaacc aaacgatgag atgctggcgt ttatgagtca tttaacctta aaagatgaag
    38581 atagccaaaa agagttggaa ttaatccgtc gtaaatggca agtttaagcc ttttatggta
    38641 tttttaagaa ccttatgata aaataaaaca gattaacaaa gattggagaa atctcatgga
    38701 agtggctgaa attcgccaaa aaatagtaga aaataaagag aagttgacta gcttcaggag
    38761 gtctctttga cttagatcgt ttggaagagg agattgctct tcttgaaaat cacatgactg
    38821 agccagactt ttggaatgac aatattgcag cgcaaaagac atctcaagaa ctaaatgagt
    38881 taaaaggtaa gtatgatact ttccataata tgcaagaatt atctgacgaa acagaactct
    38941 tacttgagat gcttgacgaa gatgattctt tgaaagaaga attagaagaa aatctaatgc
    39001 agctagataa gataatgggt gcttatgaaa tgacgcttct cttatctgaa ccctatgacc
    39061 acaataatgc tatcttagaa atccatcctg gttcaggagg aactgaagcg caagattggg
    39121 gagatttact cttacgaatg tatacccgtt ttggcaatgc taatggtttc aaagtagaag
    39181 tattggatta tcaagcagga gatgaagctg gtattaaatc ggttaccctc tcttttgaag
    39241 gtcccaatgc ttatggtttg ttgaaatcag aaatgggagt tcatcgcctc gttcgtatat
    39301 caccttttga ttcggctaaa cgtcgtcata cctcatttgc ttcagttgaa gtgatgccag
    39361 agttagatga tacgattgaa gtagaagtac gtgatgatga cattaaaatg gatactttcc
    39421 gttccggagg agctggtggt cagaatgtca ataaagtatc aacaggtgtt cgtttgacgc
    39481 atattccaac tggaattgtg gtatcgtcta ccgttgaccg tactcaatat ggaaaccgcg
    39541 atcgcgctat gaagatgttg caggctaagc tatatcagtt agaacaagaa aagaaagcac
    39601 aagaagtaga tgctctcaag ggtgataaaa aagaaatcac ttggggaagt cagattcgtt
    39661 cttatgtttt caccccttat acgatggtga aggatcatcg tactaacttt gagcttgctc
    39721 aggtagataa ggttatggat ggagaaatca atggctttat tgatgcctac cttaagtggc
    39781 gcattgaaga ctaaaaataa taaaggagaa acatggcact aatagaaatg tctggtgtta
    39841 ctaaaaaata ccgccgctca acgaccgccc tcagagacgt caatgtttct gttaaccaag
    39901 gtgagtttgt ttatctcgta ggcccatcag gtgctggtaa atcaactttt atcaaactcc
    39961 tttaccgtga agaacaactg acaactggta agctatatgt gggtgaattc aatttaacta
    40021 agctaaaagc acgtgatgtt ccgattcttc gtcgtcacat cggagtagtt tttcaagatt
    40081 acaaactgct accaagaaaa acagtctttg aaaatgtagc ttacgccatg gaagttattg
    40141 gtgaaaagcg ccgccacatt aaaaaacgcg tgccagaggt actcgattta gttggcttga
    40201 aacataagat gcgttctttt ccaagtcagc tatcaggagg ggagcagcaa cgtgtggcca
    40261 ttgcgcgtgc gattgttaac aaccctaaat tattgattgc agatgaaccg acagggaatt
    40321 tagatcctga gatttcatgg gaaattatgc agttacttga acgtattaat gttcagggaa
    40381 caactatctt aatggcgaca cacaacagtc atattgtgaa tacattccgt catcgtgttg
    40441 ttgctattga agatggtcgc atagtacgtg atgaagaaaa aggagattat ggttacgatg
    40501 attagatact ttttccgtca tatttgggaa tccattaaaa atcttaaacg caacttttgg
    40561 atgacatttg cttcagttag tatggtggct gttacattga ctttagttgg tgtttttgcg
    40621 gcgacattgt tgaatataca gcgtgttgct tcgggtgttg aaaataatgt ccatatcaat
    40681 acctacttac aagttgattc aactgatgct gcaaaagtta ttcaaaatac tgccggtgaa
    40741 cctgttaata acgataacta ccacagtgtt tacgacaaaa ttgctcagat aaaaggtgtc
    40801 aaaaagatta cattttctag caaggatgaa caattgaaaa aattgcaaga aaccttaggt
    40861 gatgtttgga atatgtatga ccaagacact aatccgctcc aagatatcta tcttattgag
    40921 acccagacgc ctaaacaagt taaggcgatc actaaaaaga tcagaacgat tgaaggcgta
    40981 gaagccgcag attatggagg gattaactcc gataaactat ttaagttttc aacattaatt
    41041 caaacttggg ggctgatcgg aacagcaatg ctattatttg tagctgtctt tctcatttca
    41101 aacactattc gtatgactat tatgtctcgt aaacgcgaca ttgaaattat gcggttagtt
    41161 ggtgctaaaa attcttatat tcgagggcct ttcttttttg aaggggcttg ggttggcttg
    41221 ttaggagctg ttttaccatc tcttctgatt tattatggat atgatcttgt ttataagcat
    41281 tttgcacaag aattacaacg taataatctc tcgatgtatc cgcttgatcc ttatgtatat
    41341 tacctgattg gtgctttgtt tgtcataggt attatgattg gttcgcttgg ttcagtgctt
    41401 tctatgagac gttatttaaa attctaaaaa agcgctgctg acgaaacgta gcgaccctca
    41461 aaagttggac ctcttatgtt attccaagga cttcgttttg tattgcacag gactggtcct
    41521 tttcattttg ttttgattca tttgtttatt tgtaataggc aatatagttt aaaatagcag
    41581 tttctaaggt ttcaagagaa gaaaagcacc ccaaaagtta gatgtttgat gtatctaact
    41641 tttggggtgt cattcaaaat ttagtggctc ttttttagta aaatactatg atttcagttt
    41701 taggataacc tcaaacagta tctaaaatag ctatgtcaat gaaaaaaggg gttagcattt
    41761 ttttcgtgac aaatggtagt agaaggacca tgcccaggat aaactcggta atgattaggt
    41821 aaggtgaata gttcttggcg aataccagta atgaggtcct caaagttact ggtaggtaag
    41881 tcagtacgac cgatagtttc acgaaataga gcatcgccag taacgactaa ttcatctgag
    41941 tggaaaacaa atgagactcc tccccaagaa tgaccaggtg ttggtaaaac tgtgaattca
    42001 aaaccattta gttgataggg ttgctttaat ttaaaaaagt tctctgccgg tcttgctata
    42061 acagtaatga tatcatcatg acggcctaag ccagataaat tatcatctgg ggaagatagc
    42121 caagcggctt ctttttctga aacataaaca ggtggatgat caaaagcatc tctgaccaaa
    42181 tctaaactaa aaatatgatc ataatgagta tgcgttaaga ggatagccac taaaggtttt
    42241 tcaaaggatt taattttagc aatgatttct tggccgttac ttcctggatc aatgaggata
    42301 acagcttgat cattaacgag gtaataagta ttttcaccag caatgtggtt tattgtttta
    42361 tagattttca taaggatagt gtaccaaaaa atcaaggctt tgttaaaaaa agagtggcgg
    42421 aaaataaatg gcaattagtt tcagaaaata tgagatctca cagcaatttc ttgacaccgt
    42481 tttcataaag gtgtatcata aaagtaatca ataagccaag cctaaagctg aacttagttt
    42541 aagaacactg actttttgag agcaacctaa tcggttgact aaaaaatatg aggtggaaga
    42601 tatgtcaaaa gttgctgttg ttactggtgc tggtcaagga attggatttg ctattgcgaa
    42661 acgtcttcat gcagacggct ttaaaatagg cattttagac tataatgaag agactgctca
    42721 ggctgcggtt gataaactat cacctgagga tgctgttgca gttgtagcag atgtttcaaa
    42781 acgtgaccaa gtttttgatg ctttccaaaa aattgtagat acttttggtg atttgaatgt
    42841 tgttgttaac aatgcaggcg ttgccccaac aacccctctt gacacgatta ctgaagagca
    42901 gtttgaaaag gcttttgcaa tcaatgtcgg aggaacgatt tggggaagcc aagcagctca
    42961 aaaacatttt agagaattag gtcatggcgg aaaaattata aatgcaacgt cacaagcagg
    43021 atgtgaaggg aatcctaatt tgactgttta cggtggtaca aaatttgcag ttcgtggtat
    43081 tacacagact ttggcaaagg atttggcatc agaaggaatc acagtcaatg cttacgcgcc
    43141 aggtattgtt aaaacaccta tgatgtttga tattgcccat gaagttggta aaaatgcagg
    43201 caaagatgat gaatggggta tggagcaatt tgcaaaagat atcactttga aacgcttgtc
    43261 tgaaccagaa gatgttgcta atgcagtagg cttcttagcg ggagatgatt caaattatat
    43321 cactggacaa acaatagttg tcgatggtgg tatggtattc cattaaaaat gattaatact
    43381 gacactgtgg gtttcactca cagtgttttt taagaagtat aaaaaattat cctatcttgg
    43441 ttattggagt gatggcacct ttgaggtata gtcatgtttt gttttataga tatagcgtgc
    43501 tataatagac ttactatgac ccagaaaaaa cttagaaaat atgcagtagt tgacttggag
    43561 gctactggag caggtcccaa tgcttctatt attcaggttg ggattgttat tatccaagga
    43621 aataaaatta ttgatagtta tgaaacagat gttaatcctc atgaatcttt ggatgagcat
    43681 attgttcatt tgacaggtat tacggataag cagttggcta aagctccgga ttttggtcaa
    43741 gtggcgcatc acatttatca attgattgaa gattgtattt ttgttgccca caatgtaaag
    43801 ttcgatgcaa atttattggc agaagctctt tttttagaag gatatgagtt aacaatacca
    43861 cgtgttgata ctgttgaatt agcccaacta tttttccctc gctttgaaaa gtataacctt
    43921 agtcacctat ctaggcagct caatattgat ttggcagaag ctcatactgc tattgcagat
    43981 gcaagagcta cagctatact gtttttaaga cttttacaaa aaattgagag ccttccaatt
    44041 gagtgcttag aaagcttatt agtctattca gatagcctgc tatttgaaac agcaatggtc
    44101 attcaagaag gattagccaa agccaaacct tatgatccca acaaatacat taaaatcaga
    44161 cagattctct taccaaaggg ctcaaaagct ctcaagcctt atcaaatatc aaaaagcttc
    44221 cctattaata tggcactatt aggtctggaa gaaaggccta aacagactca atttgctcag
    44281 ttaattgacg aagattacca tcagggagta gctagtttta ttgaagctca gacaggtatt
    44341 ggaaaaacct acggctacct gctacccctt ttggcaaaag aagatcaaaa ccaaatcatt
    44401 gtcagtgtgc ccaccaaact tcttcaagac caattaatgg ctggtgaagt agctgctatt
    44461 caagaacaat ttcacatcgc ctgccatagt ttaaaagggc ccgccaatta cctaaagctt
    44521 gatagctttg cagatagttt ggaccaaaat gaccagaacc gtttggtgaa tcgttataaa
    44581 atgcaattat tggtttggct cttagaaact aagacaggag accttgacga aatcaaacaa
    44641 aagcagcgct ttgcagctta ttttgagcag cttaagcacg atggagacat taagcagtct
    44701 tctgaatttt acgattacga tttttggaga gttagttatg aaaaagccaa aacagcccgt
    44761 ctcctgatta ccaaccatgc ttatttttta caccgtgttc aagatgacaa ggactttgct
    44821 agaaacaagg tcttggtctt tgatgaggcc caaaagctga tgctccaatt agatcaatta
    44881 tcacgtcatc agcttaacct gacggttttt ttacaaacaa tacaggccaa gttaagcaac
    44941 cctttacctc ttttagaaaa acgcttattg gaaagcttgt cgtttgaact gggacaagtg
    45001 tcaagtgatt actatcaaaa taaagagcac cagcttgctc atgattggtc tcgtatagct
    45061 ggctacgcca aagaattaac aggagcagat tatcaagagt tacaagcctt ttttgctacc
    45121 tcagatggtg actactggct tagtagcgaa aagcaagagg agaagcgagt gacttatcta
    45181 aacagtgcta gtaaggcatt tattcatttc caacagctct taccagaaac ggtgaaaact
    45241 tattttgttt ctgccacttt aaccatcagc tcagaagtaa ccttggcaga cctattaggt
    45301 tttgaagagt atctctatca tgtcattgaa aaggataaaa agcaagacca gttggtgctt
    45361 gttgatcaag aggcgcctat cgtgacagaa gtcagtgacc aaatctatgt ggaagcaatt
    45421 gcaaaacgaa tcgaaagctt gaagcaagag ggttatccta ttttagtgct ttttaattct
    45481 aaaaaacact tgctgttggt ttcagactat ctagaccaat ggcaggtgcc tcacttagcg
    45541 caggaaaaga atggaacagc ttataatatt aaaaaacggt ttgatcaagg agagcaaact
    45601 attttgcttg gtttaggttc tttttgggaa ggagttgatt ttattcaagc ggatcgtatg
    45661 attaccctta tcgcacgatt gccctttgat aacccagagg atttctttgt taagaaaatg
    45721 agtcattatc tattggaaaa aggtaaaaat cctttcagag attacttctt gccaatgact
    45781 attttgcgat taaaacaggc tattggtcgc acgatgcgtc gacaagatca aaagtcagtg
    45841 gttattattt tggatcgtcg tctccttaca aagtcctatg gacaagttat tttggaaggt
    45901 ctcggtcagg aatttttgat ttctcaacaa aattttcacg attgtctagt agaaacggac
    45961 tgttttctga tataatggaa aggatctatt atatcagaaa aatttttaat ggcgttaagc
    46021 acaatcaact aacatttaaa ttagtttgaa cttttattat gctcttttag acaactgttc
    46081 aaatgttttc gaattgtttt tttgataagg acaataaatg aacaaatttt tggttttagt
    46141 aaacccaatt actgttcttt tcattagtgt cagttgctat caaaacagct tgtcatagtt
    46201 cgtgcgatca gtagcgagta ttggtaaaac tgaaaccgta gattatgaaa aaatgatctg
    46261 ttgatcatta attaatctga agataagaag tcgataaaaa ggagatacga tgcccaaact
    46321 atcaaagcga gttttagaaa tgaaagagag tgttactctg gctgcaggtg ctagagctaa
    46381 ggctttgaaa gcgcagggcc gcgatgtact gaatttaact ttaggagaac cagatttttt
    46441 tacacctaag cacattcaag acaaggcgat tgaatctatt caaaatggta cggctagctt
    46501 ttatacaaat gcttctgggc ttccagaatt aaaagctgcg attgccacat acctgaaaaa
    46561 tcagtatggg tatcacttgt ctcctgatca gattgtggca ggaacaggtg ccaagttcat
    46621 cctttatgcc ttttttatgg ctgttttaaa tcctggtgac caagttctca ttccaacacc
    46681 atattgggta tcttattctg accaagtaaa aatggcagaa ggtcagccga tcttcgttca
    46741 aggactagaa gaaaatcaat tcaaggtaac tgttgaccag ctggaaagag caaggacaag
    46801 taagactaaa gttgtcctta tcaattctcc ttccaatcca acaggcatga tttatggtgc
    46861 tgaggaatta agggccattg gagaatgggc ggtgcacaac gatattctta ttctggcaga
    46921 tgatatttac gggtccttag tctataacgg caatcagttt gttcctattt caacactctc
    46981 agaagcgatt cggcgtcaaa ccattacggt caatggcgtg gctaaaagtt atgccatgac
    47041 aggctggcgg gttggttttg cagcaggtga accagagatc atttcggcaa tgagtaagat
    47101 catcgggcag actacctcta acctgacaac cgtttcacaa tatgctgcta tcgaggcttt
    47161 ctgtggtagt cagtcatcac ttgaagagat gcgattagca tttgaagagc gccttaatat
    47221 cacttatcca ttgttgtgtc aggtccctgg gtttgaggtt gttaaaccac aaggagcttt
    47281 ttacttcttt ccaaacgtta agaaagccat ggaaatgaca ggtttttccg atgtgacaag
    47341 ctttgctaat gctattttag aggaagtcgg attagctgtt gtgagtggag caggctttgg
    47401 cgcaccggaa aatgttcgtc tgagttatgc tacagatatc gaaaccctta aagaagctgt
    47461 tcgtagactg catgtcttta tggaaagtaa tgaaattaat taaataaaaa tagagagaaa
    47521 cttatgtcaa aaaaacttat ttcaattgtt gatgttaaag attatgttgg tcaagaagtt
    47581 actattggtg cttgggttgc taacaaatca ggtaaaggta agattgcgtt tgtacagtta
    47641 cgcgatggtt ctgctttttt ccaaggtgtc gcttttaaac caaacttcat tgaaaaatat
    47701 ggtgaagaat caggtcttga aaaatttgac gttattaaac gtctcaacca agaaacatct
    47761 gtttatgtaa cagggattgt taaagaggat gagcgttcta aatttggcta tgaacttgat
    47821 attactgacc ttgaaatcat tggagagtca catgaatatc ctataactcc taaagagcat
    47881 gggacagact tcttgatgga caaccgtcat ctttggttgc gttctcgtaa acaaatggct
    47941 gttatgcaaa ttcgtaacgc tattatttat gcaacttatg aattctttga ccaaaatggt
    48001 tttatcaaat ttgatagccc aattttatca gaaaatgcag cagaagattc aacagagtta
    48061 ttcgaaacag actattttgg gaaaccagca tttcttagcc aatctggtca attatacctt
    48121 gaagctggag caatggctct aggccgagtt ttcgactttg gtccagtctt tcgtgctgaa
    48181 aaatctaaaa cacgtcgcca tttaacagaa ttttggatga tggatgctga gtattctttc
    48241 ttatcacatg aggaatcact tgaccttcaa gaagcttatg ttaaagcatt gatccaaggc
    48301 gttctagatc gtgcgcctca agcacttgat attttagaac gtgatgttga ggctctcaaa
    48361 cgttatatca cagagccatt caagcgtgtt tcttatgatg atgctattac acttcttcaa
    48421 gagcatgaag cagatgaaga tacagattat gaacatttag agcatggtga tgactttggt
    48481 tccccacatg aaacttggat ttctaattac tttggagtac cgacatttgt agtgaattac
    48541 ccagcaagct ttaaagcatt ttatatgaaa ccagttcctg gaaatccaga acgcgttctt
    48601 tgtgctgatc ttttagctcc agaaggttat ggagagatca ttggcggctc aatgcgtgaa
    48661 gacaactacg atgctcttgt ggctaaaatg gatgaactag gtatggataa atcagaatat
    48721 gacttttacc ttgaccttcg taaatatggc tctgttcctc atggcggttt tggtataggt
    48781 attgaacgta tggtaacttt tgttgctggt accaaacata ttcgcgaagc tattccattc
    48841 ccacgtatgt tacatcggat tagaccctaa taatacaaaa agttcatgtc atctatgaac
    48901 tttttgtatt ggtaagaagt gagtgtgtta taaaaaaatc aatatgtcct aaaggagaga
    48961 taagaaatag tagagtttga acctacatcc tttgtgaaga taaattttgg aagtttagta
    49021 gatcagttag cagataagac aatatagcct tgctcaaggg atattttata gacaagtatt
    49081 tggactacga ttatgaaata gacactctca tttgatacta aaaaggtctc gtctattcat
    49141 ataataggag agctacggga gttattatta aattagctga tttttacagt tcaaaatgtg
    49201 ttaccacttt ttcacttctt agctttctta tgtatgtggt ataatgaagg tggacggttc
    49261 gatagagaaa gggaaattgt aaatgtcaga caaacacatt aatttagtta ttgtgacagg
    49321 aatgagcggc gctggaaaaa cagttgccat tcagtctttt gaggatctag gctactttac
    49381 cattgataat atgcccccag ccttggttcc aaaattttta gaattaattg aacaaaccaa
    49441 tgaaaatcgt agggtggctt tggttgtcga tatgagaagt cgtttgtttt tcaaggaaat
    49501 taattctacc ttagatagta ttgaaagcaa tcctagcatt gattttcgga ttcttttttt
    49561 ggatgcaacg gatggagaat tggtgtcacg ctataaagaa accagacgga gccacccttt
    49621 ggctgcggac ggtcgtgtgc ttgatggtat tcgattggaa agagaactcc tatctccttt
    49681 gaaaagcatg agccaacatg tggtggatac aacaaaattg acccctagac aattgcgtaa
    49741 aaccatttca gaccagtttt ctgaagggtc taatcaagcc tctttccgta ttgaagtgat
    49801 gagctttggg ttcaaatatg gtcttccttt ggatgcggat ttggtttttg atgtgcgttt
    49861 tctacccaat ccttattata aggtagagct tcgtgaaaaa acaggactag atgaggacgt
    49921 ttttaattat gtgatgtctc acccagaatc agaggtgttt tacaagcatt tgttaaacct
    49981 tattatccct atcttaccgg cttaccaaaa agaagggaag tctgtcttga cggtggctat
    50041 tggctgcaca ggaggccaac accgcagcgt tgcctttgcc cattgcttgg cagaaagtct
    50101 ggcaacagat tggtcggtta atgaaagcca tcgtgatcaa aatcgtcgta aggaaacggt
    50161 gaatcgttca tgaaaaatcc taaaatgact gttataggtg ggggtactgg catctccatt
    50221 atcctaaaaa gccttcgtaa tgaagcagtt gatattactg ctgtggtcac tgttgcagat
    50281 gatggtggtt cctcaggaga acttcgtaat gccatgcaat tagccccgcc aggagactta
    50341 cggaatgtct tgttagcgat gagtgacatg ccaaaatttt atgagcgggt tttccaatac
    50401 cgttttaacg agaatgatgg agccttggct ggtcatcctc taggcaatct cattattgct
    50461 ggtatctctg aaatgcaagg ctcaacctac aatgccattc aaattttaac caaatttttc
    50521 catattacag gtaaaattta cccctctagc gagcaggcct tgacccttca tgctgttttt
    50581 aaagatgggc atgaagtagc aggagaaagt tctattgcca aataccaagg catgattgac
    50641 catgtctatg tgaccaacac ctacaacgac caaaagcctc aggccagtcg taaggtggtt
    50701 gaggctattc tagaaagtga catgattgtc ttgggaccag gctcactgtt tacctctatt
    50761 ttacctaatt tggtcattcc agaaattaaa gaagctttgc gccagactaa agcagaagtg
    50821 gtgtatattt gtaatatcat gacccagtat ggtgaaacag aacaattttc agatgcagac
    50881 catgtggctg ttttaaatca gcacttagga agagatttga ttgatacggt tttggtaaat
    50941 gtggctaagg tgccgcaggc ttacatgaat tctaacaaat ttgatgaata cctcgttcag
    51001 gttgatcacg attttgctgg cctttgcaag gctgctaagc gtgtgatttc gtcttatttt
    51061 ttacggttgg agaatggcgg agcttttcac gatggtaact tagtggtgga agaattaatg
    51121 aatttagtga ggatagtcaa gcaatgagtt ttacaaccaa ggtcaaagag gaattaattc
    51181 acttgtccac aggtgacaac aatgaattgg ccgccattat caaattatca ggcagcttgg
    51241 gcttagctca ccaaagcctt catttatcca ttaccactga aaatgctaaa attgcccgct
    51301 acatttattc ttttattgaa gatgcctatg ttatcgtgcc agaaatcagg tatcatcaaa
    51361 agacgaacct gcgcaaaaac cgcgtttaca ccgtctatgt tgagcaaggt gtggaaacca
    51421 ttttagccga tttgaaatta gcggactcct tttttggtct ggaaacaggc atcgaacctc
    51481 aggttttatc agacgataat gctggccgtt cttatcttaa aggggctttt ctagcggcag
    51541 gtagcatcag agatcctgag tcaggcaagt atcagttaga aatctattca gtttatcttg
    51601 atcatgctca agatttagct caactcatgc aaaaatttat gctagatgcc aaaaccattg
    51661 agcataaaag cggtgcagtt acttatttgc aaaaggcaga agatatcatg gactttttaa
    51721 ttataattgg tgccatgtcc tgcaaagaag actttgaagc catcaaatta cttcgagaag
    51781 ctcgaaatga tattaaccgt gccaataatg cagaaacagc taatattgcc aaaactatca
    51841 gtgctagcat gaaaaccatt aataatatta ttaaaatcat ggacacaatt ggtttagaaa
    51901 gtttaccgat tgaattgcaa caggtagccc agcttcgggt taagcatcca gactattcca
    51961 ttcaacaagt tgctgatgcc cttgagttcc caatcaccaa gagcggggtc aaccatcgct
    52021 tgcgtaaaat caataaaatt gcagatgact tatagaacat ttttatcgta tgagcctttg
    52081 ttcatacgat ttttctgtct aaaagttgtt ttccattgtt tttttgaagt gtctgatgtt
    52141 ctgtgatatg ataaaaggga taataacgtt tgtaaaggag actgataatg gcatgtacaa
    52201 ctatactcgt cggtaaaaag gcatcttatg atggctcaac catggttgct cgaacagaag
    52261 attctcaaaa tggagatttc acgtctaaaa aaatgattgt ggtgaaacca gaagatcaac
    52321 cgagacatta ccgttcggtg caatcttcct ttgagatgga cttaccggac aatcccatga
    52381 cttacacgtc tgttcccgat gcgcttggta aagatggtat ctgggcagaa gcaggcgtga
    52441 acgaggctaa tgtggccatg tcagcaactg aaaccatcac taccaattct cgtgttttag
    52501 gggcagatcc tctagtagct tctggtattg gtgaggagga catggtgacc ttagtgctcc
    52561 cttatatccg ttctgcgcgt gaaggggttt tgcgccttgg tgctatttta gaagactatg
    52621 gcacttacga gtcaaatggt gtggcttttt cagatgaaca tgacatttgg tggctggaaa
    52681 cgattggtgg tcatcactgg attgcaagac gggtgccaga tgatgcttat gtcaccaatc
    52741 ccaatcaatt tggcattgat cattttgaat ttaacaatcc tgaagattac ctttgctcag
    52801 ctgatttaaa ggactttatt gacacgtatc acttagattt aacctatagc catgagcatt
    52861 ttaatccgcg ctatgctttt ggtagtcaac gtgacaaaga ccgtcactat aatacgccac
    52921 gtgcttggat catgcaaaaa ttcttaaacc cagaaattgt gcaagacccg cgcaactttg
    52981 cccttgcttg gtgtcaaaaa acttaccgta aaataacagt agaagatgtc aaatatgtat
    53041 tgagtagtca ctatcaggac acggtttatg acccttacgg ttcagaagga actcctgtta
    53101 gtaagaaagt ctttcgccct attggcatta accgtaccag ccaaaccgct atcttacaca
    53161 ttcgtcctaa taaacctcaa gaaattgccg ctattcaatg gatggcttat gggtcaatgc
    53221 catttaatac catggtgcct tttttcacac aggtgaagac tattccagat tattttgcca
    53281 acacttacga aaatgtgtct accgacaatt tctattggac caaccgtctg attgcggctt
    53341 tggcagatcc tcactacaat catcatgaaa ctgatttgga caattacttg gaagaaacca
    53401 tggcaaaagg tcatgccatg ttacatgctg tggaagcaca gttattggct ggtgaaacag
    53461 ttgatttgga agaggaaaat caaaaaatga gtgactatgt gcaaggagaa acgcagacgc
    53521 tgttgaacaa gattctcttt gacgctagca atctaatgac taatcgtttc agtttgagcg
    53581 attaaaagtg aagctaaggc tgagcaactg cttagccttt tttacgatta ctttttagga
    53641 aaagtaatcg caaaaagaca aaaacttgga aaaaaaatca aagtatgcta tacttaactg
    53701 gtcaattttt aaccagttaa taacaaagga gggatcatga aaaagaaaat tcttttaatg
    53761 atgagtttaa tcagtgtctt ttttgcttgg caacttactc aggcaaaaca agtcttagca
    53821 gagggtaaag tgaaggtggt gacaactttc tatcctgttt atgaatttac aaaaggggtt
    53881 attggtaatg atggcgatgt ttccatgctt atgaaagcag gaacggaacc tcatgatttt
    53941 gagccttcta caaaagacat taaaaaaatc caagatgcag atgcatttgt ttatatggat
    54001 gacaatatgg aaacttgggt ttctgatgtg aaaaaatcat tgacatctaa aaaagtgacc
    54061 atcgtcaagg gaactggtaa catgctcttg gtagcaggag ctggacatga ccatcaccat
    54121 gaggatgctg acaaaaagca tgagcataat aaacatagcg aagaaggaca caaccatgct
    54181 tttgacccac acgtgtggtt gtcaccatac cgtagcatta cggtcgttga aaatattcgc
    54241 gacagtcttt caaaagctta cccagaaaaa gcagagaact tcaaagccaa tgccgctact
    54301 tatattgaaa aattaaaaga gcttgacaaa gactatacgg cagcactttc agatgctaag
    54361 caaaaaagct ttgtgacaca acacgcagct tttggttata tggcacttga ctatggcttg
    54421 aaccaaattt ctattaatgg tgtcacacca gatgcagaac catcagcaaa acgtattgct
    54481 actttgtcaa aatacgttaa aaaatatggc atcaaataca tttattttga ggaaaatgcg
    54541 tcaagtaaag tcgcaaaaac cctagctaaa gaagcaggag ttaaagcggc tgtgcttagt
    54601 ccgcttgaag gtttgactga aaaagagatg aaagctggcc aagattactt tacggtcatg
    54661 cgtaaaaacc ttgaaacctt acgcttaacc actgatgtgg ctggtaaaga aattcttcca
    54721 gaaaaagaca cgactaagac agtttacaat ggttatttca aagacaaaga agtcaaagat
    54781 cgtcaattat ctgactggtc aggtagctgg caatctgttt acccctacct acaagatggt
    54841 actttagacc aagtttggga ctataaggct aaaaaatcta aaggtaaaat gacagcagct
    54901 gaatacaaag attactacac tactggttat aaaactgacg tggaacaaat caaaatcaat
    54961 ggtaagaaaa agactatgac ctttgttcgt aatggtgaaa agaaaacctt cacttacaca
    55021 tacgccggca aagaaatctt gacctatcca aaaggaaatc gtggagttcg tttcatgttt
    55081 gaagctaaag aaccaaatgc tggcgaattc aaatacgttc aatttagtga ccatgccatt
    55141 gctcctgaaa aagcagagca tttccacctg tactggggtg gtgacagcca agaaaaatta
    55201 cataaagagt tagaacattg gccaacttac tatggttcag atttatctgg tcgtgaaatt
    55261 gcccaagaaa tcaatgctca ttaatcttaa ctcactaagc aaatcttctt ttaaggaggt
    55321 ttgctttttc tttgtggagt aaaaaaattt aataattctc aacattaatt tgacggacac
    55381 ttatgattat aatatacttt aagtggttat aacaagttag aggtgaataa gatgccaaaa
    55441 gaacaaccac tctatctaca gattgttgat gacttggaag tgaaaatcaa aaagagcatg
    55501 acagaaaacg ataagttgtt gtcagaacga gaattaagtg acctttacgg tgttagtcgt
    55561 atcacgatta gactggctct taaggaattg gaattacgag acttaattta taaaaagcag
    55621 ggcaagggga cttatgtttc aggaatcaaa gaacctgcca ctgatttatc aagtacttac
    55681 agctttacag agcagatgaa aaaaatgggg aaaactccta aaacagagat tctttctttt
    55741 gaacagtatc aggtaacacc ttacttgtct ggtctcttag agcttgatcc cgatacagaa
    55801 gtttttgaat tagaacgcct aaggattgca gatgatatgc ctttgatgtt tgaacgatcc
    55861 tatattcctg ctcagctttt tcaagggtta agcattgagc acttgaagcg taagccaatg
    55921 tacgatttat ttgcagagga ttaccaagaa actattcgcc tagcagaaga agatttttat
    55981 gctagcattg ccttggacta tgaagcagac cttttgggaa tcaaaaaagg agatcctgtc
    56041 ttacatatta ttcgtaaaac ttataatgat aaaaatatct taattgaatt gactttcagc
    56101 attgcaaggg cggatcagtt taggtaccgg tgcaacatca ccctaatggt taaggaattg
    56161 gaggttagga catgtttaca aaaacgcagg aagctttgga ggctcttggt gcagctatca
    56221 ctaccaagga aattaaacaa gaaccccgtt tatggcaaga aaccatgacg ttttttgaag
    56281 aaacaagaga ttctctggat tcttttttaa agagagtttg taacagcgct aacagaaata
    56341 acgttcatgt catctttaca ggtgcgggta catctgagta catcggtaat accatttgcc
    56401 cttacttgaa gaaggtgggg aatcggcagc gctatctctt tgagagcgtt gcttcaaccg
    56461 atttggttgc ggcgccagat tattatcttg ttgaggaaga gacggtacta ttagtctcct
    56521 ttgctaggag tggtaatagt ccagaaagtg tagcggctgt taacctggtc aatcaactag
    56581 tgccaaatag ttatcattta accattacct gcgccaaaga tggagaactg gcgaaaaaag
    56641 cacagcaaga tgagcgttct tacctttatt tgatgcctga agacgctaat gatgctggtt
    56701 ttgccatgac tggtagtttt acctgtatga tgctagcggc tcttttgatt tttgatgatg
    56761 ctaacagtat agctcagaaa gaagcttatg tgactgatat gattcttgct gggcaagctg
    56821 ttattgacca agaaagacag ctacaagagc tagctgattt aggatttgaa cgtttagttt
    56881 atctaggttc tgctagttta gccaagttaa cccaagaagc tcaattaaaa atgttagaat
    56941 tgacagctgg acaagttgcg accctttacg agtcctctat gggctttagg catggtccga
    57001 aatcttttat taatgaccgt accttagtca ttggatttgt caataatgat gcttacgtca
    57061 gacagtacga tttggacatg ttagaggaaa tccaagcaga tggcattgcc cttaaaatct
    57121 tggctctatt gcaaggaggg gatatcaact tttcagagga ccaatttcga ctagacaccc
    57181 gtcacttatt gccagaagct tacttagctt ttcccatgat tttagtcgcg cagaccttgg
    57241 ccctcttaac ggccgttaca ttaggaaatt cccctgacac cccatctgcg acaggaacgg
    57301 ttaatcgtgt ggtcaaagga gtaacgattc acccttaccc gactagtagt cgtcaatgca
    57361 acaaagctta aagcttttgt gtagaagccg taaaacgaaa tgattttgtg ctagtaggcc
    57421 aaaagaaaaa ccctcctgat acaggaaggt tttttgtatt gtcttgctta ttttgctgcg
    57481 tttgcgtctt tgagaccgta ttttttgttg aagcggtcca cacgtccatc tgcttgagtg
    57541 aacttttgac gtcctgtgta gaatgggtgt gaatctgatg aaatttccac acggataagt
    57601 gggtaagttt caccttcaaa ctcaacagtt tctttagaag cttttgttga gccgctaagg
    57661 aactggtaac ctgtagttgt atctaggaaa acaactggac gataatctgg atgaatgtct
    57721 tttctcatct taaaaaattc ctttctgcca tgacacttga tgcatcatag ttattaacta
    57781 ttctagtgta acaaaacttg aatgacttga caaggctttt ctttccttat tttttaaaaa
    57841 tcctaaatct cttggcaaac agcgatgagt tctcggaaaa tagcttgatt ttcttctaaa
    57901 ttagctgagt tggctccgct agcaagggga tgcccccctc caccgtgacg cttagcaatg
    57961 ccattaataa tcttttcttt actgcgcata cgcacacggt agttgccgtc agttaactca
    58021 acaaaaatag cccaagcttg aacgttatca attttaccag gagcacagac aatggcagag
    58081 ctttctgcta gggtcacgtc aaaatgtttt aaggtttctt ggctgacaag gacataagca
    58141 gccccactct catcaattgt taaatgctca aagacgtagc tttgcagctt tgctattttc
    58201 aaaggaaacg aatccatttg ccttgaaatc gcagcaaagt cgaactcgaa atgtctgagt
    58261 tggctagcaa tggaaagggt tttactagtg gttgaggcat aaagaaatcg ccctgtatca
    58321 ccaacgatac cagtgtataa gagcttagca gccttgtcag agagagtaag attctgacta
    58381 aaggcaaagt ctgcaatgat ttcacttgcg ctagaagcgc ttgtgtccac ataatagaag
    58441 tcaccataca catcatcgtt gggatggtga tcaatcttaa ttaagcactg ccccagtgtg
    58501 tagcgctcat catcaatcct tggtcgattc gctgtatctg taatgatgac caaagcctct
    58561 ttatagtctt tgtcagtcac ctgatccatt tggctcatcc aagctaaact aggctcatca
    58621 aaaccagtca tcaaaacctt tttatctggg aaattttgtg caataatttc tttcaagccg
    58681 gcctgactac caagagcatc agggtcagga ttttgatggc gatggataat aatagtttgg
    58741 tgagctttta ttttatctaa aattgtttca aaagttgtta tcatgcttta atctttcttt
    58801 ttcaaaaaga ggaaaataac cgtttaacat agtcttcaaa aaacggtttg tgtcataagg
    58861 aggtgtgcgt tatgttaagt ggctaatgct agaatttttt aagattgtca aagaccataa
    58921 attgacattt tatctgaaaa tcatacccca ttttattata gcataaaagt aagctctaga
    58981 taatggtaaa ttaatctagg aagaaaagag gttagttatc agttatgata ctatcaaagc
    59041 tagtcttgca aatgcaagtg aaaaaatagt ttttaagtgt tttaagtaat catatcaacc
    59101 ttctcgttaa acaattagtg cttttcctaa aaaagtgtgc taaaataaac atagtttagt
    59161 aattttggga ggaatttatg gcattagcaa aaatcgttta tgccagcatg acgggaaata
    59221 ctgaagaaat cgctgatatc gttgcaaata aattgcaaga attgggccat gatgttgaca
    59281 ttgatgagtg cacaacagtt gacgcttcag agtttgagaa tgctgatatc gctgtcgttg
    59341 cgacttatac ttatggtgac ggtgacttac cagatgaaat cgttgatttt tacgaagatt
    59401 tacaagattt agatttagaa ggtaaaatct atggcgttgt cggctctggt gacacttttt
    59461 atgactattt ttgcaaatct gttgatgact tttcagagca atttgcgctt actggtgcca
    59521 tcaaaggtgc agagccagtc aaagtagatt tggcagcaga agatgaagac atcgaccgtt
    59581 tagaggcctt tgcagagcaa ctctcacagg ctgttaataa tctataaaac tatttagtaa
    59641 aaatgtcagg gacttccttg gcatttttag atttttaacc taaaataaag ggaatgtcaa
    59701 ttcagggtag gaattgaaaa aaacaatcag cttcaccttg cttctgatcc tcttattaac
    59761 tataataaaa agataatcta atacatctag aaaaggaaag ttatgcgttt agaagagata
    59821 cgacaagaaa tcaatagtat tgatcaccac ttggttgcct tattagaaaa gcggatggca
    59881 cttgtagagc aagtaacagc ttataaacta gccaatcact tgcctgtctt agatcaggcg
    59941 agggaaaatc agatcctaga tagggtttct tatctcgtta aagaccaagc atttgagcca
    60001 gctatccatg agacttttaa aaccattatg tctctttcac ggcagtatca aacgcaacgc
    60061 ttaaaaggtg gtgacactaa tgattaaatc aattgcaaca ctgcctaaac cctccttaaa
    60121 agaagcgtta ggactaatag gcttggcgct tcccatagga gccacggttg gagtggttga
    60181 tgtgattttt ggtaaagggc ttttgtttct ttccgaatat cgcgatcacc accttttctt
    60241 gcttcttcct tttcttgccc tagcaggtct tgttatcgtt tttctttacg ataagttagg
    60301 caaagaggtt cgtcaaggga tgggattggt ttttcaggta gggcatggtc aaaaagatca
    60361 gattcctcct attctcattc ctcttatttt gttctcgact tgggtaaccc acttatttgg
    60421 agcgagtgcg ggtcgagaag gggtagccgt tcaaatagga gctaccatct ctcattattg
    60481 tcagagattt gtgactagtc aagaagccgc acgacacctt ttgattatgg ggatggcagc
    60541 cggttttgca ggactttttc aaacgccgat tgctgctgtc gtatttgccc ttgaggtttt
    60601 gttggtagga acgcttcgct actgcgcttt attgccttcc ttagttgctg cttgcgtggc
    60661 ttcttggacc tctcattctt tgggattaga gaaatttacg attgttcttg aagaaacatt
    60721 gaccataact cccttaacct tagttaaatt aattgggtta ggtcttattt ttggtctggt
    60781 gggaaatagt tttgcctgtt tacttggctg gtttaaacct tacctgagtc aaaagctacc
    60841 aaattcttat ctccggattg ctttcattgg tgttttgtta tctatctgtt tgatgattgg
    60901 tcacgttgga agatatagtg gtctagggac tcatttgatt gcggcagctt tttctggcca
    60961 aactatccta acttacgact ggcttttaaa aatgatcgtg acggtgatta gcttatctgc
    61021 aggctttcaa ggtggtgaag tgacgcctct atttgctatt ggagcttctt taggcatcgt
    61081 tttggcccct tacttaggtc ttcctgtact gttggttgct gcacttggtt acaccacggt
    61141 atttggaagt gcaacaaata ccttttgggc tcctatcttt attgggatag aggtctttgg
    61201 tccagagaat gccctagcat attttgtcac ctcggctgca gcctatatgg ttagtcacag
    61261 acattccatt tacagttatc aaaaagtagt catgatcaat ttaaaagaca tgacggtaaa
    61321 tcagatggat ttaaaaaaat agctttacaa taaagaagct ttttgttaga ataaatctcg
    61381 tgtgtaatgg acacacgaaa attacggcta atccgctaag acaagtactt aagattagta
    61441 agataggaga ataaaaatga atccattaat ccaaagtttg acagaaggtc aacttcgctc
    61501 tgatatccct aacttccgtc ctggtgacac tgttcgcgtt cacgcaaaag ttgtcgaagg
    61561 aactcgcgaa cgtatccaga tctttgaagg tgttgttatt tcacgtaaag gtcaagggat
    61621 ctcagaaatg tacactgttc gtaaaatctc aggtggtatc ggggtagagc gtacattccc
    61681 aatccacaca ccacgtgttg acaaaatcga agttatccgc cacggtaaag tccgtcgtgc
    61741 gaaactttac tacttgcgtg cattacaagg taaagctgct cgtatcaaag aaattcgtcg
    61801 ttagtttgaa ccgaaacgta acgtaataga ataaaaagac tagagtctct agtcttttta
    61861 tgtcgtcgtg acaatgtgac aattggtgat taacgctatg ctcgtaggac actttgaaag
    61921 ttccttagca gttataatta gtatattgta ataacaaaat gaagcaaaat aaactttcag
    61981 ttatctgata tttcatcttg cattacctta ttttctaggt taaaatagaa gacaaggtga
    62041 atataagata ggccccttag ttcaatggat ataacaactc cctcctaagg agtagttgct
    62101 ggttcgattc cggcaggggt cattattgtc actgaaaaag cctttacatc aaaggctttt
    62161 ttgtattact caattaatgt tagaagcaca aaagaggcag ttcgattatt aaccaattcc
    62221 tccctattta cagttgtatg gctataaatg gacaatctta agttagggtc tgagtgacta
    62281 aacctgtccc taaccgcttt tggtggtaat cctttttcag ctaaaagcga aatgtgcgag
    62341 tgtttgaata agtgagtatg gtaatcacca taaattttta ggcgcttatt gatgtgtgca
    62401 tttaaaattg gtaaaccttt tgaatatgga aagctgttag cactctgctt gtgattaatc
    62461 tatcgtatgt taaaagcgac atttaaatta aatcattttt tatacatata tttcaaaagg
    62521 atttatcata ttggaaaata gcggtgcttc tattggcatt ttactattga caggaatttc
    62581 cctgcctttt gtctcctaaa gaagatcagc tttgatttct aacttgatta gagtgggctt
    62641 ggtactgttt atgaattatc agtgcatact ttctggagat caaaaaggaa aggagcagct
    62701 aaagaggtgt atgtgttacc aattagagga atagaacatc gcaagactcg tttattcagt
    62761 cattacgata cctgttttat ttgggaaata actataatat taagtgaatt tgtgatataa
    62821 tgaaaggtat gaattatcaa gattatatat gggatttggg aggtacctta ctcgataatt
    62881 acgagctttc aacccaagct tttgtccaaa cgctagcttt tttcagcctt ccaggagatc
    62941 acgatgctgt ttaccaaaaa ttaaaagaat caaccgctat cgctgtggca atgtttgctc
    63001 ctaatgagcc agaatttttg catgtgtata ggctaagaga agcagacaaa ttggcacaac
    63061 ccatttggtg tttgggagct aaagaaattt tggaaaagat agcgacttca ggttcgcgaa
    63121 attttttaat ttctcatcga gattgtcagg tgaaccaact tttagagcaa gctgggttgc
    63181 taatctattt tacagaagtc gtgacagctt caaatggctt tgctcggaaa ccaaatcctg
    63241 aaagtttgtt ttatttaaaa gaaaagtatg atattaatag tggattagta attggagatc
    63301 ggctaattga taaacaagca ggacaagcag cgggttttaa taccttgctt gttgacggta
    63361 gaaaaaacct attggagata gtaacgtaga tgattgaaga aaataaacat tttgaaaaaa
    63421 aaatgcaaga atacgatgcc agtcaaattc aggttctaga agggctggag gctgtccgca
    63481 tgcgtccagg gatgtatatt ggctcgacag ctaaagaggg tttgcatcat ttagtctggg
    63541 aaattgttga caactcaatt gacgaagcat tagcaggttt tgcctctcat attaaagtct
    63601 ttattgaagc agataattcc attacagtag tagatgatgg ccgtggaatt ccagttgata
    63661 tccaagccaa gacaggacgt cccgccgttg aaacagtttt tacagtctta cacgcaggtg
    63721 gtaaatttgg tggaggcggc tataaggttt ctggaggatt acatggtgta gggtcatctg
    63781 ttgttaatgc tttatcaaca caattagatg tacgtgttta taaaaacggc caaattcatt
    63841 accaagaatt taaacgcggg gctgttgtag cagatcttga ggtcattgga accactgatg
    63901 tgactggaac aacggtacac tttacacccg atccagaaat ttttaccgaa acgactcagt
    63961 ttgattacag tgttttagca aaacgtattc aagagttagc ctttttaaac cgtggtttaa
    64021 aaatttccat tacagataag cgctcaggta tggaacaaga agaacatttc cattatgaag
    64081 gtggaattgg ttcttatgtt gaatttttaa atgataaaaa agatgttatc tttgaaacgc
    64141 ccatctatac agatggtgaa ttagaaggta ttgcagttga agtagccatg caatacacga
    64201 ctagctatca agaaacagtc atgagttttg ctaataatat tcatactcat gaaggtggaa
    64261 cgcatgaaca aggctttaga gcggctctta ctcgggtcat caatgactac gctaagaaaa
    64321 ataaaattct taaagaaaat gaggacaatt tgacaggaga agatgttcgt gaaggtttga
    64381 cggcggtaat ttctgttaag catccaaatc ctcaatttga aggtcaaacc aaaacaaaat
    64441 tgggcaactc agaagtggtt aagatcacta atcgtctctt tagtgaggcc tttcaacgtt
    64501 ttcttttgga aaacccacaa gttgctcgta agattgtgga aaaagggatt ttggcttcta
    64561 aagctagaat tgcagctaag cgagcccgcg aagtcacccg caaaaaatca ggcttagaaa
    64621 tttcaaactt acctggaaaa ttagcagact gttcgtcaaa tgatgctaac caaaacgaac
    64681 ttttcatcgt cgaaggagat tcagcgggtg ggtcggccaa atcaggtcgt aaccgagagt
    64741 ttcaagctat cttgcctatt cgcggtaaaa ttttgaacgt ggaaaaagca actatggata
    64801 agattcttgc caacgaagaa attagaagtc tctttaccgc tatgggtaca ggttttggtg
    64861 cagactttga cgtgtcaaaa gctcgctacc aaaagctggt tatcatgacc gatgccgatg
    64921 tggatggcgc tcatattaga accttgcttt taactttgat ttaccgcttt atgaggcctg
    64981 ttctagaagc tggctatgtt tacatcgccc agccacctat ttatggtgtt aaggtcggta
    65041 gtgagattaa agagtatatt cagccaggta ttgatcaaga agaccaatta aaaacagctc
    65101 ttgaaaaata tagtattggt cgttcaaaac caactgttca acgttataaa ggtcttgggg
    65161 aaatggatga ccatcaactt tgggaaacta ctatggatcc tgaaaatcgt ttgatggcgc
    65221 gtgtgacagt tgatgatgcc gcagaagcag ataaagtatt tgatatgtta atgggagatc
    65281 gtgttgaacc aagacgtgat ttcatcgagg aaaatgcggt ttatagtaca ctggatattt
    65341 agtcaccttt ttagtttcac aaggaatctg ctaccaaaga ggacgaattg actaaaaatc
    65401 atgatgcatg atgccaacag ctttgttagc tagtacgtct tcatcttgag ggacagcgtt
    65461 aatgcatagg tattgagatc gccccttaat acctatgtta gtccttatta agaaagcctt
    65521 gtgagtcgct ggtacgagat tgttgaatta gtcaaatata ttttgaaatg cattttaaag
    65581 tatgttataa tcgtaatgtt atagtgtatt ttaaataaaa ttttaataaa aggcgattga
    65641 ggaatttcag tttttagtta ttactggaac aatgttgata aatcgtgtct aaataataag
    65701 gagattcaga tgtcaagcgg aattatcttg ctgattgtgg ctatagtcct actagtgatt
    65761 atcgcctatc tagtgggtgt catcatacga aagcgtaatg attccctaat taccagtcta
    65821 gaagaacgaa aacaggcctt gtttgctttg cctgttaatg atgaaattga agaagtaaag
    65881 tcacttcatt tgataggaca gagccaaacc tcttttcggg aatggaatca gaaatgggta
    65941 gacttaactg taaattcgtt tgcagatatt gaaaatcata tttttgaagc tgaaaatttg
    66001 aatgatacgt ttaattttat tcgcgccaag catgagatta atagcgttga aagtcagctt
    66061 aatcttgttg aagaagatat cgcttctatt cgtgaagcac ttaatatctt aaaagagcaa
    66121 gaagaaaaaa atagtgctcg tgtcacccat gctcttgatt tatatgaaaa gctccaagct
    66181 tctatttctg agaatgaaga taattttggt tcaactatgc ctgaaattga caaacagatg
    66241 aaaaacattg aaactgaatt ttcacagttt gtcgcactca actcatcagg tgatcctgta
    66301 gaagcttctg aagttctgga tagagcagaa gagcatacta ttgctctagg gcagattaca
    66361 gagcagattc cagctattgt ggccaagttg gaagatgatt ttccagatca gttagatgat
    66421 ttggaaacag gatatcgccg tttacttgaa gaaaactatc attttccaga aaaaaacatt
    66481 gaggcacgtt ttcaggagat tcgtgagtct attcgtgcca attcatcaga gttagttaca
    66541 cttgacttgg atcgtgctag agaagaaaac acgcatattc aagaacgcat tgattctctt
    66601 tatgaagtct ttgagcgcga aattgccgct tataaggtag ctgctaaaaa tagtaagatg
    66661 ctacctcgtt acctagcaca tgtgaaacgt aataatgaac aactcaaaga tgaaattgca
    66721 cgtttatcac gtaaatatat tctaagtgaa acggaaagtc tgactgttaa ggcatttgag
    66781 aaggatatta aagagatcga agacagtaca cttgctgttg cagaacaatt tggtttacaa
    66841 gaaaaaccat tttcagaact gcaagtgact tttgaacgta gtataaagac gcttactaat
    66901 gtcgagtcag gtcaaatgga tgtttttgca gcagttaaag atattgaaaa aattgaatca
    66961 caggcacgcc ataaccttga tgtttatgtt actcagcttc atatgattaa acgttatatg
    67021 gaaaagcgtc acttgcctgg aattccacaa gactttctta gtgctttctt tacaacgagt
    67081 tcacaattgg aagctttgat ggatgagctt agtagaggac ggatcaatat tgaggcagtg
    67141 tcacgtttgt cggaagttgc gacggttgct attgctaatc tagaagattt aacctatcaa
    67201 gtggttcaaa atgctacctt gactgaacaa ttactacaat attctaatcg ctatcgttct
    67261 tttgaagcag gagttcaaag tagttttgaa catgctttga gacttttcga agtagaaaat
    67321 gattatcagg cttcttttga tgaaatttct tatgctcttg aaacagttga acctggggta
    67381 acagatcggt ttgttaactc ttatgaaaaa acacgagagc acattcgttt ttgatacttg
    67441 aaagtcgtga tattctcacg actttttatt agctttttgg accaatacgt gtttttgact
    67501 agaatcttct ttttaatgat caaacgaaga agtagtaaca gtgttttttg ctattgagag
    67561 aatagttatt ttttcaaaaa tcttttccaa aaggatttgg ttttttcagg agtttgtttg
    67621 ctaagttgat cgttttttgt ctgctttaag atgacttcta aacgtgtttc ctttagattt
    67681 attgcgtggt cggtatgaag gataaacgca aaaggtgaag tcatatgcgt ttctgtaata
    67741 atagtaacag gtaaatggtt ttcttttgct aatttcatat agagtatttg aagttgaggt
    67801 gccagcttgg aagagatttt taagtgtaaa ggttgataga ctaactgaag gctcggtaat
    67861 tctcttttca aatagtcttt tactgtttta gtttgagctt cgtctaataa agcacttaaa
    67921 acgactcgtt cagcatacgt tcctagatag tagcgttgct gctcaggatt taatcttctt
    67981 tctccccagg ctccttttaa tagcttatct tctaaattag tcatagatac cctcgcttct
    68041 ttataatagt ttctttatta tatcgatttc tacaataaaa taccaatttg aaaattttcg
    68101 gtaaatgtgt taaaaaacaa gaaagaaagt gatttttctt gcaaaaaaca aaggaagctt
    68161 ttcatgagat tgctttttaa gttgattaaa ggcctagttt gtggtattat gaacatgtaa
    68221 aatattaaaa ctcataagga gagtattaca atgtcaatta ttactgatgt atacgctcgc
    68281 gaagtccttg actcacgcgg taacccaaca cttgaagtag aagtttatac agaatcaggt
    68341 gcattcggac gtggtatggt tccttcagga gcttcaactg gtgaacatga agccgttgaa
    68401 cttcgtgatg gtgacaaatc tcgttacctt ggtctaggta ctcaaaaagc agttgataac
    68461 gttaataaca ttatcgctga ggcaatcatc ggttacgatg ttcgcgatca acaagctatc
    68521 gaccgtgcaa tgatcgctct tgacggtact cctaacaaag gtaaacttgg tgctaatgct
    68581 attcttggtg tttctatcgc tgttgctcgt gcagctgctg actatcttga agtgccactt
    68641 tacacttacc ttggcgggtt caacactaaa gttcttccaa ctcctatgat gaacatcatc
    68701 aacggtggat cacactcaga tgccccaatt gcattccaag agttcatgat catgccagtt
    68761 ggtgcaccta ctttcaaaga aggtcttcgt tggggtgctg aagttttcca cgctcttaag
    68821 aaaattctta aagaacgcgg acttgttaca gctgtaggtg acgaaggtgg atttgctcct
    68881 aaatttgaag gaactgaaga cggtgtagaa actatcctta aagctattga agcagctggt
    68941 tacgaagctg gtgaaaacgg cattatgatc ggttttgact gtgcatcatc agaattctac
    69001 gacaaagaac gtaaagttta cgactacact aaatttgaag gtgaaggcgc tgctgttcgt
    69061 acatctgcag aacaagttga ttaccttgaa gagttggtta acaaataccc aattattact
    69121 atcgaagatg gtatggatga aaacgactgg gatggttgga aagttcttac tgaacgccta
    69181 ggcaaacgtg ttcaattggt tggtgacgac ttcttcgtta caaacactga ataccttgct
    69241 cgtggtatca aagaaaatgc agctaactca atccttatca aagttaacca aattggtact
    69301 ttgactgaaa cgtttgaagc tatcgaaatg gctaaagaag ctggatatac tgccgttgta
    69361 tcacaccgtt caggtgaaac tgaagattca acaatcgctg acatcgcagt tgcaacaaac
    69421 gctggccaaa tcaaaacagg ttcattgtca cgtacggacc gtattgctaa atacaaccaa
    69481 ttgcttcgta tcgaagatca acttggtgaa gttgctcaat acaaaggtat caaatcattc
    69541 tataacttaa aaaaatagac ggtaagacag aaattggtca ttcgtagaat gcgatttcgt
    69601 cgtcgcaccc ccgtcaggct gactaggttg gtcacgacaa aaggagtgga catccagtca
    69661 gctacgacgc aaaagttaaa gattaaagaa tagaaagctt gggaaaatcc caagcttttt
    69721 gctttctaaa tagtaaaaat ttactgtggg ctaatttatc ctgagctaat gtattagcga
    69781 ggtaaagtta ttgaggaagg cttggatttg tactgcctcc aaaaagttgg acactatatt
    69841 ttaaatacag aactaagtcc ttttatacca attgtcaagc acatcaaggt tttaactgaa
    69901 aaaatgtttt agacaatagc ctaggtttag ctgttgtctt acttgttgtg ccctgattag
    69961 cataaaaaag tgtacaagaa aaaaaacctg cctagtattt ttaagatcaa ggtgttacaa
    70021 tgatagagca acaacgactt taatgatttt gggtcgaagc gtaattgtaa agtttgttat
    70081 gcgttatgag gtaaagcatt atcctgacaa ggcaatgtct agaggcaatc gtatgtggct
    70141 tggttgaagc tgtttgcgat tgcctttttc gtttctcata gaagtctgcg atatgacaag
    70201 gattggtatg actagcagaa gcaatgtcat ggatacactt gaaaagaatc tttggagcat
    70261 aaggattccc acgtttggta atgtgctctt gagctagata gttgccagac tcataatgtc
    70321 taaagtcaat cccaatgaaa gcattaattt gattagcaga ttgaaaacga cgaatagcac
    70381 cgagtttgcc aataatactt attgcagttg tttctgcgat acttggaata gagcgtagaa
    70441 tcttgtcttc tggtaaaggt tgagccaacg ccaccatctt atcaaaaata gcttgacgac
    70501 gttcagaaag tctaagtaat tctttggcat agtagcgaac ctcttctatc atcggagagg
    70561 ttttcgtaac agtaaaataa gactgcttgg ctaatgagac aagtttttcc actaagtaga
    70621 tcccacgctt gtcagagata cgtttagagg tagattgacg gatgatagct gacaatgctt
    70681 tgtcagctag ctctagtaca aagctattac atggaaaagc cgttaccaca ttccagtatt
    70741 actcaccagt tggtgcaaat aagatgtgtt ccagttcagg aaaagtgact tgtaagatct
    70801 tgtgaaggcg attcttaact ctcgaacaat attatcctca gtcaagtttt gatagaagcg
    70861 actcaagtca cgtagcagct gataaacttc ttcctgaacg taagttggtt ttctaactag
    70921 aataaattga gactgagcca atttttcagt gtctaactta tccgctttac gcacccgtag
    70981 actatctagt tttttaaagg attgagttgt gtataagcgt agccattttt ctcaaggaag
    71041 gactgaagcc gacgagaata gactcctgtt gtgatggtct cccttttgcc ttttaaacaa
    71101 tagtatagcc gtttttcaaa atgttattaa tgattttttc ctttattcta gaagaataag
    71161 tctgatactg tttcttatta ataatctcaa tgccctaaca atccatcagt cgaacggtat
    71221 ttctcaaatt actgcagttg actcttgcgt tcagttccct caccttgtgg atgccgttta
    71281 gaacaatatt agcttgctga gccttttggt aatgtgaaca aagcatcttt atcactcaag
    71341 gagaagacag gttcttgtgg taagtgtcca acaaggtcac gataaactca tcgtaccgtt
    71401 tttggcggaa gtctagaaat gccttatgaa tgtaagcctc agaaacgtct ctaaaactaa
    71461 acaagaaata caagaacaaa tgcccttgat tctggtacaa atgctgtatc aggttcaggt
    71521 attgcctatc tgcgataatg acactctgct tttccttaac aatatctttg aaaggaatga
    71581 tttcgacatt gacaggcata accgttgcaa tagctggcgg gaagtaaatc gagagtgcat
    71641 tggtcatcaa atccagcgca gcagtgttat tggtcaaaag atagacaggt atcggtggca
    71701 aatatgtttt taggagttcc tctaactgta tcgtgaagag cagtaaatga taggacggca
    71761 gtttccccac gatgccttct gcgctcatcc attcctcaac gatggttttt agagtattgt
    71821 agaagctctt gtttcgtttt tgctgtcttc tgaggtaatg gaacaagtgt atatcaggta
    71881 acaaaacttg aagattactg ataaagagac gtgagaagga aaccaaaatc cgtttgaaag
    71941 gaagggagtt taggatatta tcgcctagta gtggacgcag ggatgctttc aaggtttttc
    72001 caattgtttt tgtcatcacc aactcaaaca attctttcac ttttttaggt gtccatttat
    72061 ccttatggaa aggattattg gtggtgcaaa aacataagaa aatataatcc aaatcctcat
    72121 cagttaaatc aggatggtac cattttccga tgatttcctg actggtcatt ttgatatcgt
    72181 gatagataga cagttttttc aagtgtttga atgcatctgt ctgaggaatc cccaagttat
    72241 catgtcgccg tttccatata agggatatca gaatggcaaa gtacgaaaat tcataagggg
    72301 ctttactggc atcattaagt gtgatggctt gattggagtt tataatcagc gaaaccactt
    72361 tattcattga tgttttatca aaatcataaa tggtcatacc aaactgatag tgcaacaagg
    72421 cgatcagaaa gcggatacgg tattctggtc caacaacatg gttttgccga acgtttaaac
    72481 ctaccttttt caagtatttc cgacaattgg tcctgatacg gtaagcagtg gcttgggaaa
    72541 tatactctcg tttaatgaag tcgtgtaggc ttagctctcc tggttcaatt aaaaaacata
    72601 acatctttaa gaatatcgag gtttgataga gtttcttaaa ataaggcatt ttaagttggt
    72661 aggtcttata ggggatagaa acagtgtcgt cacaaatggt taaggtgagg gtgtcttcta
    72721 aatccttaat catttcaatg agtaagttgt agacacgttg ttttttgagt tcggtttcct
    72781 gacacaactg gtctatggag acttcggaaa cgtctaacaa aatgttgagc agtgataatt
    72841 tatcttgtaa tttggtttct agatgaaggt gtaacataac tgaactcctt tcatttttat
    72901 agcagaaa
//
LOCUS       DAUCWU010000003        68400 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUCWU010000003 DAUCWU010000000
VERSION     DAUCWU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.003"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(64..427)
                     /locus_tag="VGY24_000159"
                     /pseudo
     CDS             complement(64..427)
                     /locus_tag="VGY24_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(848..1381)
                     /locus_tag="VGY24_000160"
     CDS             complement(848..1381)
                     /locus_tag="VGY24_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ4283182.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1461..2237)
                     /gene="recX"
                     /locus_tag="VGY24_000161"
     CDS             complement(1461..2237)
                     /gene="recX"
                     /locus_tag="VGY24_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ4283183.1"
                     /translation="MKITKIEKKKRLYLIELDNDDSLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEVLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2352..3707
                     /gene="rlmD"
                     /locus_tag="VGY24_000162"
     CDS             2352..3707
                     /gene="rlmD"
                     /locus_tag="VGY24_000162"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ4283184.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITAVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLARFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVAKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
     gene            4371..5726
                     /gene="rocA"
                     /locus_tag="VGY24_000163"
     CDS             4371..5726
                     /gene="rocA"
                     /locus_tag="VGY24_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ4283185.1"
                     /translation="MLEDFLQFLGFIFLDIIEIMLTLKLFSFVSAIPLRLKNIFYLSL
                     SMVLFQVVFWAFFPDHFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSIS
                     LVKRFIVFFVMPLFGMPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQ
                     SHRASKLLVFTNSSMALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIH
                     LERYVKQNSIEAIVQQKEYRELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQND
                     ISIVRLTYEGILNAEKNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVS
                     IPNCIATFFLEVVDFTKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTK
                     QGQDDSQSVFAIPALKKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKP
                     D"
     gene            complement(5827..8532)
                     /locus_tag="VGY24_000164"
     CDS             complement(5827..8532)
                     /locus_tag="VGY24_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ4283186.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLGYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKKYHFGQRFPKEVYQEAQEYLARLSQ
                     SFQIIDTSGQVRPEAEILGTSIAFDYDLPKRSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETVSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISCQPNHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPVSADKLLQA
                     EMEAVIDITERQSGRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPNAAFWKNPSNPQHQECFVSLFDGEKGVTIGNYGLNE
                     YEILPDSNTIAITLLRSVGEMGDWGYFPTPEAQCLGQHSLSYSFESIDRQTQFASYWR
                     AQEGQVPILVEQTSHHTGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(8547..9833)
                     /locus_tag="VGY24_000165"
     CDS             complement(8547..9833)
                     /locus_tag="VGY24_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ4283187.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLFTGDIPAMWLRDSTAQMKPYLFLANEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANEKGHQTDHTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVMLSYLEDLYNNLFHNESVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            9966..11006
                     /locus_tag="VGY24_000166"
     CDS             9966..11006
                     /locus_tag="VGY24_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4283188.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPQLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            11057..12751
                     /locus_tag="VGY24_000167"
     CDS             11057..12751
                     /locus_tag="VGY24_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ4283189.1"
                     /translation="MTIYHTLKDYQEVMTRGDYLVFDTPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFPMRGIIEGFYGTPWTREERLDCLRFIGNKRM
                     NTYMYAPKDDDYQRKQWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEIAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQPDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVELVNTLRKLDKPAFQQAIAPWFERVAKDQVFWQAM
                     LNQEPQLETLYADVQEHNYRIGSDIPSRYYRIYYQQRDKLTANQGQVTQAIPEDYA"
     gene            12776..14218
                     /locus_tag="VGY24_000168"
     CDS             12776..14218
                     /locus_tag="VGY24_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4283190.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAADFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(14829..15707)
                     /locus_tag="VGY24_000169"
     CDS             complement(14829..15707)
                     /locus_tag="VGY24_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4283191.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAVNKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQRDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            15903..16853
                     /locus_tag="VGY24_000170"
     CDS             15903..16853
                     /locus_tag="VGY24_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4283192.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLVGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            16863..17789
                     /locus_tag="VGY24_000171"
     CDS             16863..17789
                     /locus_tag="VGY24_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ4283193.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            17870..19315
                     /locus_tag="VGY24_000172"
     CDS             17870..19315
                     /locus_tag="VGY24_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4283194.1"
                     /translation="MTKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            19621..20226
                     /locus_tag="VGY24_000173"
     CDS             19621..20226
                     /locus_tag="VGY24_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ4283195.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            20216..21940
                     /locus_tag="VGY24_000174"
     CDS             20216..21940
                     /locus_tag="VGY24_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283196.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDKVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            21940..23424
                     /locus_tag="VGY24_000175"
     CDS             21940..23424
                     /locus_tag="VGY24_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4283197.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAATLTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            23354..26860
                     /locus_tag="VGY24_000176"
     CDS             23354..26860
                     /locus_tag="VGY24_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ4283198.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPNSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIYLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFTANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENASYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEKVSQMATLLSQLDHPQGLIGLNYADGKDYDRLHEEHSDWLLYGSET
                     VSAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDSPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKASQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKASTPHILITSYQHY
                     LPLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLAASQKRLTYCYQYDTVQAIGA
                     ISLQNHTSTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLAVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            26998..27876
                     /locus_tag="VGY24_000177"
     CDS             26998..27876
                     /locus_tag="VGY24_000177"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ4283199.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            28111..28866
                     /locus_tag="VGY24_000178"
     CDS             28111..28866
                     /locus_tag="VGY24_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ4283200.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            28919..29257
                     /locus_tag="VGY24_000179"
     CDS             28919..29257
                     /locus_tag="VGY24_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ4283201.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEV"
     gene            29259..29564
                     /locus_tag="VGY24_000180"
     CDS             29259..29564
                     /locus_tag="VGY24_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ4283202.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPKDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(30000..31073)
                     /locus_tag="VGY24_000181"
     CDS             complement(30000..31073)
                     /locus_tag="VGY24_000181"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ4283203.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQTKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRSLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKQIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(31129..31881)
                     /locus_tag="VGY24_000182"
     CDS             complement(31129..31881)
                     /locus_tag="VGY24_000182"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ4283204.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(32122..32192)
                     /locus_tag="VGY24_000183"
     tRNA            complement(32122..32192)
                     /locus_tag="VGY24_000183"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32158..32160),aa:Thr,seq:ggt)
     gene            32359..32613
                     /locus_tag="VGY24_000184"
     CDS             32359..32613
                     /locus_tag="VGY24_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ4283205.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            32631..33026
                     /locus_tag="VGY24_000185"
     CDS             32631..33026
                     /locus_tag="VGY24_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ4283206.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASLKK"
     gene            33302..33592
                     /locus_tag="VGY24_000186"
                     /pseudo
     CDS             33302..33592
                     /locus_tag="VGY24_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            33711..36359
                     /locus_tag="VGY24_000187"
     CDS             33711..36359
                     /locus_tag="VGY24_000187"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ4283207.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDNRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFNSNINYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMQKIKS"
     gene            36513..38921
                     /gene="cas3"
                     /locus_tag="VGY24_000188"
     CDS             36513..38921
                     /gene="cas3"
                     /locus_tag="VGY24_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HEQ4283208.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQSMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDMTDRPVYQLSTNMCAQHRLDLIAKIETELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFEATKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            39054..39782
                     /gene="cas5c"
                     /locus_tag="VGY24_000189"
     CDS             39054..39782
                     /gene="cas5c"
                     /locus_tag="VGY24_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ4283209.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLSAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGINIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            39782..41677
                     /gene="cas8c"
                     /locus_tag="VGY24_000190"
     CDS             39782..41677
                     /gene="cas8c"
                     /locus_tag="VGY24_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ4283210.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFSYYSAEMG
                     QTQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQHNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKALHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLMI
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            41682..42530
                     /gene="cas7c"
                     /locus_tag="VGY24_000191"
     CDS             41682..42530
                     /gene="cas7c"
                     /locus_tag="VGY24_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ4283211.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            42532..43206
                     /gene="cas4"
                     /locus_tag="VGY24_000192"
     CDS             42532..43206
                     /gene="cas4"
                     /locus_tag="VGY24_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ4283212.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            43203..44204
                     /gene="cas1c"
                     /locus_tag="VGY24_000193"
     CDS             43203..44204
                     /gene="cas1c"
                     /locus_tag="VGY24_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ4283213.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLESYPPFMV"
     gene            44215..44508
                     /gene="cas2"
                     /locus_tag="VGY24_000194"
     CDS             44215..44508
                     /gene="cas2"
                     /locus_tag="VGY24_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ4283214.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   44657..45149
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=44657..44688
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            45309..46019
                     /gene="ccdA2"
                     /locus_tag="VGY24_000195"
     CDS             45309..46019
                     /gene="ccdA2"
                     /locus_tag="VGY24_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ4283215.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRSFLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            46032..46655
                     /locus_tag="VGY24_000196"
     CDS             46032..46655
                     /locus_tag="VGY24_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ4283216.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKGLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            46698..47804
                     /gene="msrB"
                     /locus_tag="VGY24_000197"
     CDS             46698..47804
                     /gene="msrB"
                     /locus_tag="VGY24_000197"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ4283217.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNVPVDQKVIYLAGGCFWGVEEYFSQVNGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVIRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            47892..48632
                     /locus_tag="VGY24_000198"
     CDS             47892..48632
                     /locus_tag="VGY24_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ4283218.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLDLSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            48629..50362
                     /locus_tag="VGY24_000199"
     CDS             48629..50362
                     /locus_tag="VGY24_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283219.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNSEIQDFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDIAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDHFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            50435..51514
                     /locus_tag="VGY24_000200"
     CDS             50435..51514
                     /locus_tag="VGY24_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ4283220.1"
                     /translation="MNLATDYFSRQTPIVEKLVAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPKR
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKRNWISIILDDKVTDDQLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETDIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            51528..52244
                     /locus_tag="VGY24_000201"
     CDS             51528..52244
                     /locus_tag="VGY24_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ4283221.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLAES
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(52410..52883)
                     /locus_tag="VGY24_000202"
     CDS             complement(52410..52883)
                     /locus_tag="VGY24_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4283222.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            53025..53705
                     /locus_tag="VGY24_000203"
     CDS             53025..53705
                     /locus_tag="VGY24_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ4283223.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFTYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
     gene            53979..55214
                     /gene="arcA"
                     /locus_tag="VGY24_000204"
     CDS             53979..55214
                     /gene="arcA"
                     /locus_tag="VGY24_000204"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ4283224.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESSYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            55314..55742
                     /locus_tag="VGY24_000205"
     CDS             55314..55742
                     /locus_tag="VGY24_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4283225.1"
                     /translation="MADKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            55782..56795
                     /gene="argF"
                     /locus_tag="VGY24_000206"
     CDS             55782..56795
                     /gene="argF"
                     /locus_tag="VGY24_000206"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ4283226.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            56965..58458
                     /locus_tag="VGY24_000207"
     CDS             56965..58458
                     /locus_tag="VGY24_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ4283227.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            58475..59806
                     /locus_tag="VGY24_000208"
     CDS             58475..59806
                     /locus_tag="VGY24_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ4283228.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPKGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTEPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEICLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAAQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            59826..60776
                     /gene="arcC"
                     /locus_tag="VGY24_000209"
     CDS             59826..60776
                     /gene="arcC"
                     /locus_tag="VGY24_000209"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ4283229.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            61089..62081
                     /locus_tag="VGY24_000210"
     CDS             61089..62081
                     /locus_tag="VGY24_000210"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ4283230.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLGF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKQHIGEVQTSVWPQEVRDSYDNIL"
     gene            62204..62743
                     /gene="rsmD"
                     /locus_tag="VGY24_000211"
     CDS             62204..62743
                     /gene="rsmD"
                     /locus_tag="VGY24_000211"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ4283231.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            62733..63224
                     /gene="coaD"
                     /locus_tag="VGY24_000212"
     CDS             62733..63224
                     /gene="coaD"
                     /locus_tag="VGY24_000212"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ4283232.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            63211..64248
                     /locus_tag="VGY24_000213"
     CDS             63211..64248
                     /locus_tag="VGY24_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ4283233.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            64662..65639
                     /locus_tag="VGY24_000214"
     CDS             64662..65639
                     /locus_tag="VGY24_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4283234.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            66063..66707
                     /locus_tag="VGY24_000215"
     CDS             66063..66707
                     /locus_tag="VGY24_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ4283235.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISPFIIRKKDK"
     gene            66711..67790
                     /gene="rlmN"
                     /locus_tag="VGY24_000216"
     CDS             66711..67790
                     /gene="rlmN"
                     /locus_tag="VGY24_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ4283236.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(67872..68264)
                     /locus_tag="VGY24_000217"
     CDS             complement(67872..68264)
                     /locus_tag="VGY24_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEQ4283237.1"
                     /translation="MVHWVSASYCYLLLFSLLFSIFDWRSQEYPFILWLFSFVSLLLF
                     YSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALVLDLTSLIWLIQLASLAGITA
                     CLLLGIKRIPFIPYLSFGLFWIVLLEHF"
BASE COUNT        20852 a        12471 c        14632 g        20445 t
ORIGIN      
        1 aaagacactt tacaatgctc tcaagtcatg tgttatggta actttagcag ttacagtaag
       61 acaaaaggtg acttggaaaa gcttatgcag acgattctta actcccacaa tatcttctgt
      121 cagattttat tagacttctt ctcagtgtca attttgtctg ttttacgaac gtgcaggcta
      181 ttcaattgct tcttagcttc taaaggatta aaccgagtat aagcgtagcc attatttgcg
      241 aaaaaacctg aagacgacga gaatagacat taatcgtttt aaggataatc tcagggttat
      301 ggacggtttt caagtttcca agaagatgat tgaggccgat ggtatcgttt tgtcttgaat
      361 gattccttat tttagtggtt tcattttcaa tacacgacgt caagcgtcct acatactttg
      421 ataaaattct acctaaaaga ggtgtcttgt cagttttggt tacaacatca agtgccaaaa
      481 gactttgcga cttaacaaga cacttctact ttaacgcaaa gaaaaagtag tgagtactct
      541 ctttcgtcgg agatttcctc gctactaaac ttagtatttt ttgatacttt ccttaggtag
      601 tgcttaaggc agcgactttc tttggaattc catgccttat ttttgagatc ctaggctcaa
      661 aaatgccaag tggcgtcaac attaacattg acgccacttt taccacaacg aaaaacatct
      721 gttttggctc actccacacg cagaaatagg aaattggatg tcaaatcagc agagctgttc
      781 atgctttttg agcctaaaac tgagtttgtc tgcgttctga gcactccttg ggagtgctgg
      841 acaactctta acgattcttc agttcaagat aacgtttgta ccaaatagtg atataggcct
      901 tggaaaatgg tcctttttca tgattaatcc aatcaaccaa tattttgaca ttttctttta
      961 ggataaagtc catatcggct gaatactgca tctctttttt atgaatctcg tattcatcaa
     1021 catcaagtaa tctcttttca ccatccgcaa agactttgac atctaaatcg taatcaatgt
     1081 atttaagggc ctcagtgtcc atcatgtatg gactagctaa gttacagtaa taagagacgc
     1141 cattatctcg aatcatggct ataatgttaa accagtattt tttatgaaag taaacgattg
     1201 caggttcgcg ggtcacccat cgtctaccgt cactttctgt gacaagagtg tgatcattaa
     1261 ctccaatgag ggcattttca gttgttttta gtaccatagt atcgcgccaa gtacggtgca
     1321 aactaccatc atgcttataa ctttgaattg taataaagtc gccttcttta ggtaatttca
     1381 tgccttacca actttctaca attaaactta tcctctatag tctatcataa atgtgtgaaa
     1441 aaatcacttc ttttgtcagt ctataaataa tttcttaact tgcaattaat atcgtcgctg
     1501 ttgtagcctt ttcggtagag agcctgatag agcttttgct ttaaggtata accatcatat
     1561 ttgcgactga gtttacgata ttgtttgtct aattctttgt caagaagaac ttctatttct
     1621 tgattatctt ggtcaaaatt aaggtgattg aggctatgtt tagctaggtc gtatgaaaag
     1681 cctttggtca gtaatgcttg ggtgatttta tctttcaagg cttttggtgg caatttttct
     1741 tgatatttgt caaagagttt ttggcttact ttttgagcga gttggctaaa gtcagtttga
     1801 gataagatag gatcaatgtc atgacttgca atgccttttt gtaataattt ttgttttaag
     1861 acttggggac ctttatcacc atttaactga ttttggcgaa tgtaggtatc agccaatttg
     1921 gtgtcgtcta tccattgttc ttcttggagt tgagtgatga tgtcagcaat aatgtgttct
     1981 tcaatctcat gcttgcgcag gtaatcagca acttgcttgt tgctgcgttg ttgaaaggaa
     2041 agaaaataaa gggctaaatt tttgccgtag gacagttggg caaaatgttt catgtcttca
     2101 agctgatcat tgtcaaggac tttatcttta ctcaacataa accgaacaat agtatcttct
     2161 gttacataaa gggaatcgtc attatccaat tcgataaggt agaggcgttt tttcttttca
     2221 attttagtga ttttcatatg ttcattatat caaaaatggt aaaatagaag atgaaaatac
     2281 tgctaaaaat gtgttgcttg tgaatgcttt tggtggtgag attaggatat tagaggaact
     2341 aaggggaaga gatggtcgta aaggttaaac agaaaattcc tttaaaaatt aagcgaatgg
     2401 gaattaacgg tgaggggatt ggtttttatc agaaaacttt ggtatttgtc cctggtgctt
     2461 taaagggaga agatattttt tgccaaatca cggcggttaa acggaatttt gcagaggcca
     2521 aactgttaat ggtaaacaag gcttctaaga atcgtgtcaa accagcttgt tctgtttatg
     2581 aaacttgtgg tggttgccaa atcatgcatc tagcctatcc taaacagcta gatttcaaag
     2641 atgatgtgat taggcaggcc cttaaaaagt tcaaaccaac tggttatgaa caatttgaga
     2701 ttagaccgac tttaggaatg aaaaagccag atcactatcg ggccaaattg cagtttcagc
     2761 tgcggtcatt tggcgggacg gtcaaggctg gtttattttc ccagggaagt catcgcttgg
     2821 ttcctattga caattgcttg gtacaggatc aactaacgca agatattatc aataagatta
     2881 cgcagttagt ggacaagtac aagttgccca tttacaatga gcgcaagatt gctgggattc
     2941 ggacgataat ggttcgtaag gcacaggcta gtgatcaggt acagattatt gttgtgagta
     3001 gcaaagaagt taggttagcg aggttcattg gcgaattgac taaggcattt cctcaggtta
     3061 aaacggttgc cttgaatagt aatcgttcta aatcgagtga gatttatggg gacgaaacgg
     3121 aaattctatg gggacaagag gcgattcatg aagaagtttt agactatgga tttgcgttat
     3181 caccgagagc tttttaccaa cttaatccac agcagacaga agttttgtat ggtgaggtgg
     3241 cgaaggcctt ggatgtaggg agcaaagatc atattattga tgcctattgt ggggttggct
     3301 cgattggctt tgcttttgca gggaaggtca agtctgtgcg ggggatggat attatcccag
     3361 aagcgattga ggatgcacaa aaaaatgcca aagccatggg ctttgacaac gcttactatg
     3421 aggcagggaa ggcagaggat attattccta aatggtataa acaaggttac cgcgcggatg
     3481 ccatcattgt ggacccacca agaactggtt tagatgataa attattaaaa acgattcttc
     3541 attaccagcc taaacagatg gtttatgtgt cttgtaatac ctccactttg gcccgcgatt
     3601 tggtgcagct aacaaaggtt tacgatgttc actatatcca aagtgtagac atgttccccc
     3661 atacagctag gaccgaggct gtcgtgaagt tacaaaaacg tgtatgattg agtggcaata
     3721 acaactcaag ttttataagc tcaggtaatg ggtttgagca atagcttgac atagtaggga
     3781 tgtcaaaaac ggtttactaa gcatagtcat attattgata actctatgtt tattttagaa
     3841 acattcatgg ttaaaaaagt ttgttagttt gtcactaagt agggtctatt tgaagagcaa
     3901 gtacacacag acaatattag gggaattgat tgttgaaaaa agctgttttt tgctataaat
     3961 tagaaaactg agtggtcagg aggttacatt accagaagtt attttagtat gctaatatga
     4021 acgatgatat agaggattta tcctggttta atcttttaag ctgtaagaaa cctttgcaaa
     4081 aactgtaggc tgtgtgagtc tttatgtgtt agatattaaa gataaatgca acactttttt
     4141 ataatgagat caacaactga taaatctaag ttgataacca gtaaaatgat cggaatgtag
     4201 aaaagttact taatcaagct tttcgtaatt taactgttag aatgacagaa cttatgataa
     4261 aataaagaat atgattaaaa tgaaaaagct cagtttatct attacggagt tcgaaatgaa
     4321 aagaaaatcg agatgtgata acatattttg aacgagaagg agaaggataa atgttagaag
     4381 attttcttca atttttagga tttatatttt tagatattat tgaaattatg ttaacgttaa
     4441 agctttttag ctttgtaagt gcaattccgc ttcgcctgaa aaatattttt tatttatcac
     4501 ttagtatggt tttgtttcaa gtagtttttt gggctttctt tccagaccat ttcatccttg
     4561 atgttgtgat gttggctcag tttcttttct ttgcgcttat agccctatac tatggtaaat
     4621 caataaaagc taagttttta atgttttatg ccttttttcc actagtgtct attagtttgg
     4681 ttaagcgatt tattgtattt tttgttatgc cattgtttgg tatgccttat tcagttgtta
     4741 aacataatac actactgatt tacagcatta cttgctttag tatttttttg atttatcgct
     4801 gcattcaagt ttttcatttc gatttttcaa cctggcgcca atattttcaa tcacatagag
     4861 ctagtaaact attagtgttt actaactctt cgatggctct atattacttg tgtgtccaag
     4921 ggatagatgt gatgtcgcct tctttatcag gacttgctac aacgactgct cgttcaatca
     4981 tagtgctctt ttatttcatt ttgtttctta ctttattaat tcatttagag cgttatgtaa
     5041 aacaaaactc tattgaggca attgtgcaac aaaaagaata tcgtgagctg attaattata
     5101 gtcagcacct tggattgctg tatcaagata ttcaagagct taggcagcta ttaactaccg
     5161 tgtccagtcg tcttaagatt ggaatcgaac aaaatgatat ctctattgtg agacttactt
     5221 atgaaggtat cttgaatgct gaaaagaata atgctaaaga tgacagactt gatttaactt
     5281 gtttagataa attacaagtt gaagcaatca gacatattgt tttagctaaa ttaattgagg
     5341 caaaaaataa gaagcttaag gttgaggtat cgatccctaa ttgtattgca acgttttttc
     5401 tagaagtagt agatttcact aagctattgt catttttgtt agataatgct atagaaatga
     5461 gtttggagac aaagcaacct tgcttatcaa tagcatttct ggatcaaaac cataaacttg
     5521 tcatagtcat tcaaagcagt actaaacaag gacaagatga tagtcaaagc gtgtttgcta
     5581 taccggcttt gaaaaaaaga gatgactggc aatttgactt aaggaatgtc acgaccattt
     5641 taaatcgtta tgactatctc acaattagct cgcagattca tgatggcatt ttaacccagt
     5701 taatagaaat agctaagcct gactgatgtt aaaggtatga ataatcactc aaaatcaact
     5761 taagagtctg agacaatagt cccagactct ttttgaatat gttaatctat tactaatgat
     5821 aatagtttat tgtttcttcc aagctagagt taaaatttcc gctttgccaa gttggctggg
     5881 tgtgctttgc ttgctgtctt tttctaacaa attagtgacc ttggcattgt agtttggcag
     5941 gcttaggcta aagtcacaag ttttatcgtt tgagagatta tagctgcgcg tgataagggc
     6001 attgtctgct aagcgacgtt tgaaagctgt tagagcgact tgatcgtctg caccagtgac
     6061 atagctatgc tctgctctta agcttcccgt atgatggctg gtttgttcga ctaggatagg
     6121 aacttggcct tcttgtgccc gccaatagct ggcaaattgt gtttgcctat cgatgctttc
     6181 aaaactataa gataggctat gctggccaag acactgggct tcaggtgttg ggaagtaacc
     6241 ccagtccccc atctcaccaa cagaacgtaa gagagtgatg gcaatggtgt tgctatctgg
     6301 taaaatctca tattcgttga ggccgtagtt accaatagtg accccctttt caccatcaaa
     6361 gagactcaca aagcattctt ggtgctgtgg gttacttgga ttcttccaaa aggcagcatt
     6421 tggatgattt ggacgtttga cagtttcaaa aatactatca gctaggtgat ggtctgtctt
     6481 aaggtgcgtt gggaatagga cgcgcaagcg atgattagtc atttggttat caaaacttgt
     6541 ggtgaattgg aggcgaggat tgtttttctc catgcggata agggttgtta aggttagctc
     6601 agccttttct tgtgaacgtc ctgattggcg ttctgtgatg tcaatgacag cctccatttc
     6661 agcctgtaag agcttatctg cggagacagg aatggcaaag gtttgctgga tttcaagttc
     6721 agcaacttga gcggtgttgc taatgatgtt aagcttggtg gtcccttggt ctgcatagaa
     6781 aggttggtca tgatttggtt ggcaagaaat atattcattt ccaatatcgc cacaatcttc
     6841 aaaccgcaac aggtcttgat agattagtcc agattgttta tcggtgatgg ttagacgacc
     6901 atcggtttgt atcataactt ttagaaaccc attttcaagg cactgattac tgtcatcgta
     6961 gagggaaacg gtttctgaag gagttgtttc atttcctagc tttaatgcta aggttttcca
     7021 agacatggct gggagagtta ttggtagccg taatctcact ttgatggcga aataaggttc
     7081 gcggaaggat ctcttgggca aatcgtagtc aaaagcgatg cttgtgccta aaatttctgc
     7141 ttcgggtctc acttgtccac tagtgtcaat aatttggaaa gattgggaga gtcttgccaa
     7201 atattcttga gcttcttggt aaacctcttt aggaaagcgt tggccaaaat gatatttttt
     7261 ccaggtcagg ctgagctcag caacacttgt tttggaatgg ccgctggtat taaataagac
     7321 gaagggttgg ctatccattg gaaaatccct ggtatcaatg gcgtcagcaa tttgcttagc
     7381 agcttctttt gctaaatagt gtccgacttc ataggctttt tcaaagcgcg tcatcatttc
     7441 ccgatgaacg ctatcaacac tacaaccaca gatagaatca tgagggtgat tttgcatgag
     7501 ggttttccaa gcgtaaccca gttggtcgtg agggtaggta cttgttacct cataagccat
     7561 tgctgctaag ggttcggtga tgttttcgag ttggcgagag actctggtat tagcctgttt
     7621 gagataaata cgagcagaag ccgtgttagc tagggtatac cagccatcgg tttcttgact
     7681 ggtaatctct ccttggacgg ttgaaaggtt ctctggtaaa tcatctgcga gatcagccaa
     7741 gtaatcttca aagcaggaat gcacaaattc gtagtcagga tagagttggt tggctaaggc
     7801 gattgccttg gtgacatcaa gttgtacggg ttgatgatca cacccgttca tcatcagaag
     7861 gtgcttggtt gaggcaaagc gttcagcgtc agcaagtttt ttatcccaaa aaagacgcgc
     7921 ctcagcttct gttgtcggga tctcattgcc attgctgtac cagttggcga agaggagtcc
     7981 aagaatacga ctgttatctg ggccttgcca actgatttca gagaattggg agctgtattt
     8041 ttcactggta tccacctgat tgttaaatcc agttggacga atgccacgac caaaggcagc
     8101 agcttgtagg ccggctttta acatcagctg tggtgtttgt cccatatttc caaaggtatc
     8161 aggaaaataa ccaagtggca cactagcgcc ccatctgtca caatcttcct taccaatcag
     8221 catattgcgc acattggatt cactgctggt caaaaaatcg tcctgtaaga tatagaaagg
     8281 tccgatacgg agttttcccg aagcaatggc ttgtctaatc tcaggttctc gttcggggcg
     8341 tacttttaaa taatcatcta ggataatggt ttgcccatcc aaatgaaaac tatgaaaatc
     8401 aggatccgtt tgaaaaactt ctaacaggtc atctattaag ttaatcagac gcatgtggtg
     8461 ttgttcgtaa gccatgtacc actcgcgatc ccagtgactg tgtgaaataa tatgtacttt
     8521 tttagttgcc atagtggcct cactttctaa ggagcaatac taaagcccag atagtcaaga
     8581 agtagttcac agaacatcat gttagcccaa gagaaccact cacgagtgta ttgataagga
     8641 tcattgacat cgaaaccctc gtgcattaag tgggtaccag catctgttgc cacaagcaaa
     8701 tcaagcatct cttttttact gtcttggtca agagcggtca atccttgtaa ggcaagggcg
     8761 atatgccaga tataattttc tggtgtatgg gaagaaccga tgcctgctgc tgcatttccc
     8821 tgataataat aagggttttc ttggctgaga atggtgcggc gcgttgccag atagataggg
     8881 tcatctttag cgcaaaaacc aaggtaaggt gcagcaagga ggcttggaat attagcatca
     8941 tccatgatgg aaaactgtcc cagaccatcc acttcataag cgtaaatggt ttctcccttg
     9001 ctgttttgaa caagggcatg gtctgcaata cccgattgaa tagcttcttt cagctgcttg
     9061 gcacgagtag ctactgattc attgtgaaaa aggttgttat ataaatcttc tagataggaa
     9121 agcataacca ccgcaaacat atttgaagga atgaggtaac catattgaca agcatcatca
     9181 cttggtctaa agcctgacca ggtcatccct gttggtgcga caggacttcc ttttccagca
     9241 tgtgttaggg tatcttcttt gcgccaagta tcgcgttcaa acaagtaagg ggactgggcg
     9301 tggtcttgct caactgtcca taggtcaaga atgagttcta ccccacgatg aaaatcgtca
     9361 ttaaactgat cggtgcttcc tgtttcacga tagagcagat aagccagttg aatagggtag
     9421 caaagggaat caatctcata tttgcgttcc caaatccaag ggttcatttg ggtatgatct
     9481 gtttggtgtc ctttttcgtt agcttcttcg ttgaaggcat tggcataggg atcgatacaa
     9541 atgtagcgga attggcgttt gaccagaccg gcaataatct ttctaatttc ttcatcttca
     9601 ttggcgagga agaggtaagg tttcatttgc gcagtggaat cacgtaacca catggcagga
     9661 atatcgccag taaaaagaaa gctagtgcca tcttcgtagg tttttagggt tgtctctaag
     9721 gtattggtaa agctggtttt gaaattaaga gcccaatcag ggtgttggtc tccacaaagg
     9781 ctttccacct tggccataaa ttggctgacg ctagttggga ttggtcgtgt catgtcatat
     9841 cctccaaaat gatatatcat ttatcttcga tttcattata aatgataagt taaactttgt
     9901 ctataagttt ttgaaaaggt ttacaactct tttttgacat gttatactga taacaggagt
     9961 aaagaatgac aaaattatta tacaaaaaga tatatcattt cattaaagag cagatagcat
    10021 caggtcgttt gcaaattggt gaccgcctac ctactgaaaa agaattgtca gaacagttct
    10081 cggttagccg tatcacttct aagcgagcgc ttgtagaact ggagcaagaa ggcttgatta
    10141 cgagaagtcg tgggaaagga agcttcgtag ctaaaaagca agtgacgtcc cctgaagcga
    10201 ataaggattt attgttgatt cttccttttg cttctgatta tgaattggga gattatgcta
    10261 agggaattat gacctccatt gctgaaacag gttaccgtct catggttcaa ttggccagta
    10321 cagtaaggtt agacaccctg tcagactatg ctgggattat ttattatcct gaagatgtca
    10381 atcacagtat tgatttcctg ttttactgcg accatcatca cattcctcta gtcttgttgg
    10441 ataaatctct agatcttttt cagtttccgt ctgttgtagc ggataataaa ggcggtgcct
    10501 gtcaattgac tcagcacttg attgagcaag gctgtgacca gattagtttt gtggcaacag
    10561 gctcttttgg agaagtatca tccgttagag accgttattt gggttactta gcggcaatgg
    10621 cagaatcgtc tctaaaatcc cattattttc ctaagacaga gactgaaaca acagatgctt
    10681 atttggaccg tttggtgaaa accttatcgg aaacgacagc cactaaaagg gggcttgtcg
    10741 tggaaaacga ttggttggct attcagctga ttcaaaaaat cacacaggta ggcttatcta
    10801 ttcctgatca agtcgctatt gtaggctttg ataatagtca agcctcccgt ttgttgagtc
    10861 cccaattaag cacagcagct caagattttt accaaatggg gcaagaagca gctaaagtct
    10921 tgttgcagat tattcatgag ccagaccaag cagtggtgtc ttgccagtta cctgttcaag
    10981 tatttatcag agaaagtagt cactaaaaaa gacgttacta tcatgagctg gttagctcag
    11041 gtaaaggaga ccagaaatga ccatttatca caccttaaag gattatcaag aagtcatgac
    11101 aagaggagac tatcttgtct ttgacacgcc attgacttgc cgatttattg gccgtttttt
    11161 ccgctttgaa aatcaaaaag ccctgctggc agaattggca acgtcaaagt attttcagtg
    11221 gattgaagaa ggccaagcag aggtgacgat gaaacacttt tttaaccgtc agctggctaa
    11281 ggatgccttt accctgaagg tttcagaaga taaggaaatc attattgaaa gccaaaacct
    11341 tagaggtttt cgttacgccc aagaagcttt gcttaaagtc atgaccttca aaggggataa
    11401 actttatttg cccattgttt ctgtcaagca cagcccgtca tttcctatgc gggggataat
    11461 tgaaggcttt tatggaacac cgtggacacg agaagagcgg ttagattgcc tgcgttttat
    11521 tggaaataaa aggatgaata cttacatgta tgctccgaaa gatgatgatt accaacgcaa
    11581 acaatggcga gacctctatc ctgaggattg ggtgacttat ttcaaagagc tacttgcagt
    11641 agccaaggaa gaagggattg atttttggta tatgattagt ccgggacttg attttgatta
    11701 taccaaggaa gctgactatc agttgctcta tcaaaaattg caacaacttc tagcgttagg
    11761 tgtttgtcat tttgggcttt tgctggatga tattgactac caaattgtag atgctgttga
    11821 gagacgattc aaaaagactg cctatgccca ggcccacttg gcaactgagg ttcatcactt
    11881 tttaaaccaa caacatgcgg cgccagaatt ggtgatctgt ccgacagaat acgacaacca
    11941 ccatgattct atctatttgc aagagctgtc agaaaggatt cctaaagaaa tcgccttttt
    12001 ctggacaggt ccaagcacgc tagcgagtca aatctcgcaa gcagatattg aaactatggc
    12061 agcagtatat caacggccta tcattatttg ggacaatatt cctgttaatg attatcaaaa
    12121 agatccagaa cgtctctttt taaccccttt tgctaatcgc tcgccatttt tatgccagcc
    12181 agactatcag gtcaagggga ttgtttctaa ccctatgatt agttgggaat tatctaagct
    12241 gaccttgaca gacatgagcc attatctgtg ggatgctaac cgttatcagc ctagtaactc
    12301 ttggctagaa accttaacag actatacagg agatgctgaa ttagccttag ctttacaggc
    12361 atttgcttgg cataatggca atcgtcatct gcacagggac cttccttttg aagtggaaga
    12421 agctctcctg gctaaggatg tatcaacgtt atcagcttgg gtagatgaat tggtagaact
    12481 tgtcaatacc ttgagaaagc tagataagcc agcgtttcaa caagctattg ctccttggtt
    12541 tgaacgggtg gcaaaagatc aggtgttttg gcaggctatg ttaaatcagg agcctcagtt
    12601 ggaaacactt tatgccgatg tgcaagaaca taactaccgt attggaagtg acattccaag
    12661 tcgttactat cgcatctact accaacaacg agataaacta acagctaatc aaggtcaagt
    12721 tacccaagca atacctgaag attatgctta gtcatacgag ctagaaagga atgatatgtt
    12781 agcatttcca aaagagtttt ggtggggagg agccacttca ggccctcaaa gtgaggggcg
    12841 ttttgctaag cagcaccgca atctctttga ttattggtat gaagaggagc ctgacttgtt
    12901 ttacgattac gtgggacctg atacggcttc ggatgcctat catcagattg aatctgattt
    12961 gaccttgtta gcttcactag gtcacaattc ctaccgcacc tccattcagt ggacacgttt
    13021 gattgatgat tttgagcaag cgacgattaa tcctgatggc cttgcttatt ataatcgagt
    13081 gattgatgct tgcttggcca atggtattcg tcctgttatt aatttgcatc attttgacct
    13141 gccaattgcc ctctatcaag cctatggtgg ctgggaatca aagcatgtcg tggacttatt
    13201 tgtcgccttt agcaaggttt gttttgagca gtttggtgat cgggttaagg attggtttgt
    13261 ccacaatgaa cctatggtcg ttgtggaagg ctcttattta atgcaatttc attaccctgc
    13321 tatcgtggat gggaaaaaag cagtccaggt cgcctataat ctagccctag ccacagccaa
    13381 ggttattcaa gcttacaggc aaggtccagc agaacttagt gatgggcgta ttggaaccat
    13441 tctcaatctg acgccagcct acccagctag tcaatcagaa gcggacatgg ctgctgcgga
    13501 ttttgcagaa ctttggaaca atgacttgtt tatggaagct gctgttcatg ggaaattccc
    13561 agaagaattg gttgcggtat tgaaaaaaga tggggttttg tggcaatcaa cgccagaaga
    13621 attggctctt attgctgaga atagagtgga ttatttggga ttgaattttt accatcctaa
    13681 gcgcgtgaag gctccggatg tcattccagt gattagccca tcttggagtc ctgaatggta
    13741 ttatgacccc tacctgatgc ctggccgccg catgaacgtg gataaggggt gggagatcta
    13801 tcctgaggct gtctatgata ttgccatcaa gatgcgagac cattacgaca atattccttg
    13861 gttcttatct gaaaatggtg tagggatttc aggagaagac cgttaccgtg atgaaactgg
    13921 tcagattcaa gatgattatc gcatccgatt tttgaaagaa catttgacct acctccacaa
    13981 aggaatagaa gcaggctcga attgttttgg ataccatgta tggacgccaa ttgatggctg
    14041 gtcttggctc aatgcctata aaaaccgcta tggccttgtg gaaaataata tccataccca
    14101 agtgcgacga cctaaagctt cagcttattg gtttaaaaaa gtagctactc ataaccgctt
    14161 gatttcatta gaagtaatgg aggagtttgg aggctctgct tctcacctca gtgattgaac
    14221 gtgtgaacgt ctcctttgag acaagtgaat aatgatgtta ccaatgatta aacaagggcc
    14281 tttttcctca tgttaagagg atcgaggctc ttgtatttct ttggaaagtg ttggtatttg
    14341 ccgaactaga tataagcgtt ctgtctggtt tagcatcaac tatccatctt gtttaatttg
    14401 actcttcaat tctttagagt atcttggcta aggatgcttg tttttcatat tggtatttta
    14461 ggggtttctt tcatattttt gggccagcta aaagcccctc atggtttcta gtgactgttt
    14521 gttatgtcac tgtccacgat aaggggacta tatgagtgag ttattaagtg aggctttgct
    14581 tattggcgat aactagcata gcttctaata gctcgagcat taactgatga ccaatgcctt
    14641 taacagaagc gtcagcaaca acgacatcaa caaagggacc aggcatcagg ctgcgttcat
    14701 cagcaaagag tcatttaggc agaaagccag tatcgttgta gctaatcagc aatcctttta
    14761 ggatgtcttt gtagatgtca gaggcgatta gggacaatag gggataagag ggatttagcg
    14821 gtccatggtt aggcctcctt gtgttcttgg taaaaatcaa tggcagctcc aagaagattg
    14881 gcttcgtttt taaagtgaca aatggctaat tggggtctaa ggtcggagat ttcgacggct
    14941 tggtagagtt tgtctagttc agcttcaatc gctgacagta gaaagtcagc ttgggaaaca
    15001 ccacccccga ttaaaatcag ttgaggatca aaggcgtgtt gaatgttaaa aataccgatg
    15061 gctaaacttt ggagaaagac ttgcctttct ttgagtgcta ggggatctct ttgttctgcg
    15121 agggcaagga cagctttgcc agtataatct tttccgttat tgacgattgc actatagcgc
    15181 ttggccatat ttaccactgt ccctaactga ctaaaggttt ggtagcggtc gttcatgatc
    15241 atgaaaccaa attcgccccc aaataagtgg gcaccatggt aaatcttgcc atcaatgacc
    15301 aagcttcctc caactccagt accaagaacg agcatggcta ggctactagc gccttttcct
    15361 gcccctaagg tagcttcagc taaggcggca caattggcat cattttcgat agaaataggg
    15421 taatgtagcc gttcttctaa ggcctcttgt attttaaaat ggtgaatgta gggaatagcg
    15481 ctggcgcctt cgataacccc cttttcttta ttgactgccc caggtgaact gatggcaata
    15541 cctgagaaat ggtaagcaga gtaacgagca acttcttggt cgagtaattg gtaaaacttt
    15601 tccagactag agggagtggg aaagctgctt tgctggctta attggccatt gtgacagagg
    15661 gcaaatttaa gggaagtgcc tcctatatca atacataaca agctcatgag gtgttctcct
    15721 ttattatagt agaatagacg atcatgtctc atcttagtat gacagattta agcaacaatg
    15781 aaaaggcttt ctaagtgatt ctatactttg tgttattata cctataattt gttgctttca
    15841 aataatgaaa gcgcttgtta tactgtaact ataacgtgat taacacaaag ggaggttggg
    15901 aaatgacaag acagaccaac aaaaagacat ccttttggaa aaatgtgatt aaataccggg
    15961 ccttattatt aatggtatta ccaggtttca tctggttcat ctttttcttt tatattccag
    16021 ttttagccaa tgtggttgct tttaaagatt ttcattattc agcagggggc tttatggaaa
    16081 gtttgaagga gagcccttgg gttggtttgg ctaacttcaa atatctcttt gcttccaaag
    16141 atgcttggtt gattaccaga aataccatcg cttacaatgt gattttctta ctttttaatg
    16201 ttttctttgc gattgctttt gccattatca tgagtgagtt acgaaataag cgaacggtta
    16261 aggtctatca caccatgtcc ttattgcctt atttcttatc atgggtggtt attgaatact
    16321 ttgtttctgc ctttttaaat acagataaag gtttcattaa tcagctcttg gttggcagtg
    16381 gagcagatcc aattaagtgg tattccaatc ccacttggtg gcccttgatt ttactcttta
    16441 tgagtgtctg gaaagggcta ggctacaata gcattatcta ctatgcttca gtcaaaggga
    16501 tttctgatac ttattatgaa gctgctatgg tggatggtgc aagcaaatgg caacaaatta
    16561 ggaacatcac cattccacaa ttattgccaa tgatgtctat cttattgatc attaatattg
    16621 gtaatatttt taaatctgat ttcggacttt tctacgttat tcccaagaat tcaggcccac
    16681 tttatgacgt gaccagtgtg ttagatacct atgtctacaa tgccttgact gctacaggag
    16741 atattgggat ggcatcggca gctagccttt atcagtcagt agttgggacc agcatcttgt
    16801 tagtcaccaa tgcgattgtt cgtcgcatgg accctgatgc agccttgttt taggaggaaa
    16861 acatggagaa aaagaaaaaa gtcgaaaagg ttaatgttag aacctttgat cgaaaaacca
    16921 atgctatctt taatattctg attggtctgt ttgccatctc ttgtatcatt ccttttatct
    16981 tcgtgattat tatttccttc actgatgaaa gttacttgat taatcatggg tatagttttt
    17041 ttccagatgt ctggtcaact aaggcttacc agtatatttt tcaaggagcc atgtcccata
    17101 gaatcatgag gtcatttggg atatccgtgt ttattacagt ggtgggaacc tttattaaca
    17161 cgaccatgac atcaacctat gcttatgcga tttcaagacc ttatttccca tacagacgtt
    17221 tttttactgt ttatgcactt atcaccatgc tctttgcacc agggatggtt gctaattact
    17281 tggtggtcag caatctcctt catttgaagg atacggtttg ggccttgatt ttaccaatgg
    17341 ctctgggccc atttggcatc ttggtcatga gaacgttctt taaaaagaca gttccggata
    17401 gcattattga atcagctcgt atggatgggg ctagtgaatg gatgattttt atgaaaattg
    17461 tcttaccatt agctgttcca gggattgcca ccatcagttt attttctgct ttaacttatt
    17521 ggaatgattg gtttaacgcc ttgctttatg tgcaaagtga gaatctttac ccaatgcagt
    17581 acttactgat gaaaattcag agcaacctac aggccttggc acaaaatgct ggcatgagtg
    17641 cacaaatggc agatagctta gcatcgctgc caaaagaatc agttcgtatg gctatcgtgg
    17701 ttattgcaac cttgccgatt gccttgactt acccattctt ccaaaagtac tttgtcggtg
    17761 gtttgaccat tggtggggtg aaggaatagg acttaaggaa ctggtcacaa gaccagattt
    17821 tatctaatca ttagtaacaa aagaaaacat tttttaggag gcttcactta tgacaaagtg
    17881 gcaaaaaatc gtgtgtgtga ctggaactgt gcttgcagcg tctagtttag cagcctgcga
    17941 aagcaagtca gcatcaaagg atagtgatgt caaattattg atgtaccaag ttggtgacaa
    18001 acctgataac ttcgatgaat tgatgacaat tgctaacaag cgcatcaaag aaaaaacagg
    18061 tgcaacggtt gaccttcaat acatcggttg gggggactgg gatgataaaa tgagtaccat
    18121 cattgcctct ggtgaaaact acgacattgc ttttgccaat aattatgtgg tcaatgcaca
    18181 aaaaggtgct tttgctgatt tgacaacgtt aatgccaaaa tacgctaaga aaacgtataa
    18241 aaacttagac ccagcctata ttaaaggaaa tactattgac ggtaaactct atgccttccc
    18301 agtagatgcc aacgtttatg cccaacagat gctttctttc aataaagaac tagtggacaa
    18361 atatggcctt gacatttcaa acattaagtc ctatgcagat gctgaaaatg tcttgaaaca
    18421 attccacgaa aaagaaccaa atacagcagc ttttgctatt ggtcaagtct ttagtatgtc
    18481 aggtgactat gactacccat taaccaaaac ccaacccttt gctgtgaaaa ttgatgaagg
    18541 caagccaacc atcattaatc agtatgaaga tgagtccttt aaaaacaatc tccgcttgat
    18601 gcacaaatgg tataaagaag gtttgattcc aacagatgca gcgaccaata cagaaggtta
    18661 tccccttgaa ggaaacactt ggtttatgcg tgaagaaacc caaggtccta tggactatgg
    18721 cgatactatc ttgaccaatg ctgcaggaaa agacatcgtg tctcgtccat tgactaaacc
    18781 gctaaaaacc acatcacaag cacaaatggc aaactttgtg gtatcaagcg tatctaaaaa
    18841 caaagaaaaa gcagttgaag tcctttctct tcttaatagc gatcccgaat tgttaaatgg
    18901 gcttgtttat ggtgtcgaag gcaaagcttg ggaaaaaatt ggcgacaaga aaatcaaact
    18961 gctcgatggc tatcaaccaa aaatgcacat gggtgcttgg aatactggta acaataaaat
    19021 cctttacact caagaatctg ttactgacga catgattgct aaacgtgacc aatcaatcaa
    19081 ggatgctaaa gaatctccaa tccttggttt cactgttgac acaaaagtta tcaaaacgga
    19141 attgtcaaat atttcaaatg tcatgaaccg atataaagca agtatcaata ctggtacagt
    19201 ggatcctgac gaagcccttc caaaacttct tgcagacctc aaaggtgctg gttgggacaa
    19261 ggtgcaaaaa gaagttcaaa aacaattgga cgactttgta gctaaagaca agtaaggcat
    19321 tcattttctt gattataagt tgagggctgg gaaatctcag ctcttttctt ctgatgagtg
    19381 gaaaaaacgg atcatcaaat aagtggaagt tataaaggga gaaaaattaa tgtaaataat
    19441 gttactactc ttagtaaagc ggtttccaaa agcctataaa gtgtgctata ttagaagagt
    19501 ctaaactatt aatcagagta aaactaatac cagcatctta ctgggggtgc taaaactagt
    19561 ctcgtagctg ctggtccttg aaaccgctat aagtgttaac aagaaaaaga ggactatcat
    19621 atgaatggca ttgaaaaaac ctgcttttta atttggaaaa tgatccaatt gaccctcatt
    19681 tttcatctct tgtccttagc tggactgatt gtgtttgggg tggggccagc ctggcaaacc
    19741 attgtcaccc tatttttaaa aacacctcaa gaagaaaacc actattcttt gaaaagggct
    19801 gttcaacttt ggaaatctta tttcaaagaa gctaatctaa gatttggtct ttttgccctg
    19861 actttccttt ttttaacctt taatttgcat tgggcagtac agtttccatc cttgttttgg
    19921 tttaccgtgt ctttcttgat tgtaatagcc atggtttggc taaccattac ctatctttac
    19981 atggtttttt atgcagtgag ctatgagatt aacctttgga ataatatgaa attagccttt
    20041 atcagcgtct ttttaagttt caaatccttt ttactcatgc tgagtgtttt gctgggaatt
    20101 accttagtga cttggcaata caaaggcctt tatctctttt taaccttcgg agctctagtc
    20161 ttttgcttgg actttgtcac taaggctaat cgtcgactag tagatggagt gattgatgag
    20221 tgttaaaaca ttgggaaggt atgtgactaa ccactttaag cagggttttt tgattaatcg
    20281 actgatgaaa ttatatagcc tgctaattgt aagttttttc actttagcag ctattggctt
    20341 gagcagttat tctattagta gtacttataa aagggtggat gctgaggcgc agatgcgact
    20401 ggaggagact ctaggtagat tgcagtcaca aaacgatatt accctacggg ttttagacca
    20461 gttggttggc cgttcagaag attaccataa tctctatcaa tacatgacgt taaccccaaa
    20521 ccagtatttt tcaaacgttt ttgaagactg ggaaaaagga aaagacacgg tcctattttc
    20581 tgaagaggtg aggcgtcttt ttgacttgta ccctgatgtg acttctatga ccctactgct
    20641 agaggacagt agtgcttatc tgtacgccga tagggtcatt aagacgggtc gtttgcttta
    20701 tagcaagcct gaaaaagtaa ccggaaatgt tttagttcgc tccattcgaa atccagaatc
    20761 aggagatgtg acaggtcgtc tttacctgac ctttgacaaa ccaccgactt tggtagagac
    20821 acagcaggat cactatttag ctacttttgc ttttgattac tatggccgca aactcttcca
    20881 tcaaggaggt cggagatttc ctcatttaga ggctgaggtt aaaaaggcta tcaaggcaga
    20941 tcgggcagct gatcttagta atctcagcaa ggcttatcgg atgcagtaca atcgctcggg
    21001 agacctctta gcttatgtgg ctgtcagaaa atcctattta ttagcggaag ctgtcaggac
    21061 agtttttgtg tatggccttg tttctctact tttagcttgg ctgttattgc agttgctctt
    21121 tagggttttc cgaaattata tccagcaggt ttctgaaatt acggatactg ttgaaatggt
    21181 tgcagcaggc gatttgtctt taaccatcga taacagccac atggaattgg aactgtatca
    21241 tatctcagaa gccattaatc agatgttggc cagcattaaa gcttatattg acgaagttta
    21301 tgtgttagag gtagagcaac gagatgccca aatgagagct ctgcagtctc aaatcaaccc
    21361 tcatttttta tacaacacgt tagagtatat tcggatgtac gcccttagtt gtcaacaaga
    21421 agaattagca gatgtcattt atgcctttgc gagtctgctt cgcaacaata ttagccaaga
    21481 taagatgacc accttaaaag aagaactggc tttttgtgaa aagtacattt acctttatca
    21541 aatgcggtat ccagatagct tcgcttatca tgtaaaaatt gatgagagta ttgctgactt
    21601 agccattcct aaatttgtca ttcaacctct cgttgaaaat tattttgtgc atgggattga
    21661 ttatagtcgc catgacaatg cactaagcat caaggcttta gatgagacgg atcatctctt
    21721 gattcaggtg cttgataatg gacgtggtat tagtcaagag cgcttagcag atatggaaaa
    21781 aaggcttcaa gagcaccaaa caacaggcaa tagctctatt ggtttgcaaa atgtttacct
    21841 ccgtcttttc catcattttc gagacaaggt ttcttggtcc atggctaagg agccaaatgg
    21901 tggctttatc attcaaatta ggattagaaa ggatgcttga tgtataaggt actattagtt
    21961 gacgatgaat acatgatttt acaagggctc accatgatta ttgattggca ggcacttgga
    22021 tttgaagtgg ttcaaacagc aagatcaggc aaggaagcct tagcctattt gactcagtac
    22081 ccagtggatg tcatgatttc agatgttacc atgcctggaa tgacaggact ggatttaata
    22141 gaagcagcta aaacctatca tcctcagcta caaaccttga ttttatctgg ttatcaagaa
    22201 ttctcctatg tgcaaaaggc tatggaatta gaaaccaagg ggtatttgct aaaacctgtg
    22261 gataaggcag aattacaggc taaaatgaaa caatttaaag actgcttgga tgcccagcag
    22321 gcagaatcaa tccggcaaga agcctatcat gacagccttt tgaccctttg gcttacagat
    22381 gaattgagtg agaaagaatt tcagcagcta agtcaaggct tgccagcggc tactttaact
    22441 ggttttacag tgctgtattt ggactgtcaa gagtggcaag cagccatcga tgcttatttt
    22501 gaacgagaag gtcagccttt ttacctcaaa aaagaagaag ataagaaggt ttatctggct
    22561 gtcctacttg gtaaaactga tcaagccaag gcatttacct atgatctaca aaagcgattc
    22621 tcaagaaaca tgcatcaact tattttggga gaaacagttg atgactggga aaatgtttat
    22681 gaaagctaca atcaggtgct taagagcctc ttttacaatt gtgaagtgtc accaattaga
    22741 gcaaaagata agtccaaaat tgaactacca gagtcccgtt tgcagttctt tgcttttaac
    22801 aaggcgttga tgattgggga cgaacctaca atcttgtcta aattagaagc tatttttgac
    22861 gagatgaaga ccttgaactt ttcaccagag gatgtgaagc atgtttcgtt cttgttgttt
    22921 tctgacattt accgtcaatt tcccatttta gataaaatga cttatctgtc catggtaaaa
    22981 actattcatg acagccaatc cattgatcgc attttgaggg aactaaaaaa agtgttggat
    23041 gtcaccaatc agaacaattc ccctgaaaaa cgttattcag atttagttag tgaaaccatt
    23101 gattgcattc gcaaggaata ccatcaagaa ttaaccctta aagctatcgc tgataggtta
    23161 catgtcaatg gggtttattt agggcaatgc tttaaaaatg aaaccgagcg tagttttacc
    23221 cagtacttaa atcatgttag gattcaaaaa gcgcaacagt tattgcttta caccaaccaa
    23281 tccatcaatg aaattgctta tgaaacaggt tacaatacca atcattattt tatcaagatg
    23341 tttaaaaagt tgaatggttt atcgccaaaa gagttcagag atcgttacaa ggacaattac
    23401 caagccatca agggcgacca gtaacgtcaa acaggagatt gatatgacac caaatagcaa
    23461 ggagcagaca gtgactggct accaatacca ctatattgat caagaaggaa gaaagcagcc
    23521 ctttaatcaa ggctggcgtt ttcttatggc agatgtggcg tgtgctcagg acccttcttt
    23581 tgatgattcg aactggcagg tgatttattt accacatgat ttcagtctta cgcagcctta
    23641 cacaagaaat ggtgaagctg aaagtgctta taaattaggt ggtgtcggtt ggtatcggca
    23701 ttatctcgtg ttagatgagg ttttagctgg ttgtcatgtt gccattactt ttgagggttc
    23761 ctatatggaa acggaaattt atgttaatgg gcaattcatt ggcaaacact taaatggcta
    23821 tcaggaattt acctatgata tctctgatgt agtgactttt ggagctgaaa atcttctggc
    23881 tgtccgggtt gaaaacaagg ttcccagttc tcgttggtat tcaggcagtg gtctttatcg
    23941 tgaagtgagt ttgtctgttc ttccccagct tcattttgtg gcagaccagg tagcaatgac
    24001 cttggctgat actgctgtgc aggagaaagg gcaacagaaa gttgatttac gctttgccct
    24061 taatcagtct attcagactt gccactacca attgagcctc tgtttatggg aacaatcaca
    24121 ttgctcaaaa gacaagaaac tcctttatca agaaacagaa gtgcctctag ctgatttggc
    24181 tttccaaaga cagtatggtc ttacactgtc tttggaagaa ctccaattgt ggtcaccaga
    24241 taatcctcac ctctacgatt tagaactcac tctttactat caaggtcagg tcattgattg
    24301 cttttgcctt gaaactggtt ttcgtcagct gacttttacg gccaatcagg gcctttttgt
    24361 gaatggcaga gctgtcaaat taaagggagt ttgtttgcat catgaccaag gaggcctagg
    24421 agcttgtgct tacgaagatg ccctagccag acaattagtc ctgttgaaag acatgggggc
    24481 taatactatt cgtagcaccc ataacccaag tagtcccaaa cttaggcaac tagccaatcg
    24541 tctcggcttt tttgtgattg aagaagcctt tgacacctgg acctatgcca aaaatggaaa
    24601 tgttaatgac ttttcaaact atttccatca gactatcgga actgaaaatg caagctatct
    24661 tcaaagagtg aggtcacctg aaacgagctg ggcccagtat agtattgagg ccatggtctg
    24721 gtctgccaag aatgacccgt cagtgttgat gtggtcgatt ggtaatgaat tgatggaagg
    24781 tttttcagcc gatgtcagtc attacccaga gctgacaaga caaatgtgcc aatggattgc
    24841 tgctattgac acctcacgtc ccattacctt tggggacaat aaattaaaag aagctgactt
    24901 ttgctggcat gagaaagtat cgcaaatggc aactcttctt agtcaattag accatcctca
    24961 gggactcatt gggttaaact atgctgatgg aaaagactat gataggcttc atgaggagca
    25021 ctctgattgg ctgttgtatg gttctgaaac agtctcagcc attaccagca gggcttatta
    25081 taaagagaca aaaaaagttc ttgactccgg ttatcaccta acctcttatg atcatgctaa
    25141 ggttgattgg ggtgccttcg ctagccaggc ttggtatgat accattaccc gtgattttgt
    25201 tgctggcgaa tgcgtttgga caggctttga ttatttagga gaacctaccc cctggaacaa
    25261 gatcgacagc ggtgttgtag gcctttggcc ttctcctaaa aatgcctact ttggcatttt
    25321 agacacggca ggctttccaa aagatagcta ttatttttac caaagtcagt gggcgcaggg
    25381 gcagaccacc ttgcacctgt taccagtctg gcagaaggat cagctttgtt tcgatgagca
    25441 gggattggtg gaggttgtgg tttatagcaa tgctgccagt gtgcaactga tgtttgaaga
    25501 tgagcagggc aacttaacag attatggaag gaaggcattt catacttata gcacgccaac
    25561 gggacacact tatcagcttt accaaggagc tgacgctgct aagaatcctc acgagaatct
    25621 ttacctgact tggcgagtgc cctatcaaaa agggttgcta agggcagtgg cctacgacat
    25681 ttctggtaaa agcattccaa aaaccagtgg acgttctcag gttagaacct atggttcagt
    25741 ggcaaagcta tcttggaaag cctttgaagc acctattgac tctccttggg aattgcttta
    25801 tctggattta tcactcttgg atagccgagg tgaattggtc agccatgctc aagattggct
    25861 aaaggttcaa gtagaaggac cagcccgttt gttggctctt gataatggga atccgactga
    25921 ccacacccct tatcaggagc ctctacgcca ggcatatggg ggaaaattat tagccatttt
    25981 agccctcact ggagaggcgg gacacattaa ggtcacggca aaagcctcgc agcaacttag
    26041 cagtgttttt cagacacaag tagcctgccg tgcccccaaa gaacacttag tgacactacc
    26101 tttagagaaa tatcagttta aaaagagccg actgacaagt caacaagaaa ttggtcaatc
    26161 ttgtctaaag accacacaat tcctttctgt tttggagaac aaagacaccc agcaaaaagc
    26221 aggtaaggct agcacacctc acattcttat caccagttac cagcactatt tacctctgcc
    26281 cttgcaggtt tgttctcagg catttgattt taaaaaaggc gatgatatct tcttgcctca
    26341 gtggctccaa gtcaaagatg cccaaggttc ttttgtgaaa aaagcttttc caatgacatg
    26401 gtacctaaaa gagaaaatct ttgccaagcc tattcttgtc acgggctatg tttcttgttt
    26461 tgggcaatca ataccggttg aggcaaggct taacctagca caagctagca aaagacggca
    26521 ccaggatctc agtttagtcg ccaagcaatc cctggccgca agtcaaaaaa ggctaactta
    26581 ttgctaccaa tatgatacgg tgcaagccat aggagccatt agcttgcaaa atcacacaag
    26641 cacatctctt agtgtttctg tggcaatcag ttatggtagt cagagctcta ttaagcaaga
    26701 aaccttgatg ctagctgata gccacttgag ccagttagaa gaggttattt tcttcgatga
    26761 tattttgact gttttgtatc tggcagtgac ccttgaagtc attgaaggac ctgacctaac
    26821 tgaagacagt attcaaattg gcctttggga tttagcatga agcatgagta aaagaagact
    26881 tctttggcac caggtctgat gaattctgta aaataaggta taataaaaaa caaataggcg
    26941 tcctttatta cgtgaaaggc gcctttacaa ggttgaaatg gaaagtgaga tttctaaatg
    27001 tcagaacgtt tatcaggcca caccctgttg gtctctttgc ttgctacccc tattcgtcat
    27061 agtttgtctc ccaaaatgca caatgaagcc tatgctaaac tggggctgga ttatgcctac
    27121 cttgcttttg aagtagggac agagcaatta gcagatgccg tgcaagggat tcgtgcctta
    27181 ggaattcgtg gctccaatgt gtctatgcca aataaagaag ctattttacc cttattggat
    27241 gacttgtccc cagctgctga gttggtcggt gcagtcaata cggttgtcaa taaggatggt
    27301 aaaggacatc tcgtggggca tattacagac ggcataggag cgctcagagc cttggctgac
    27361 gaaggagtgt cggtaaagaa caaaatcatt accctagcag gagttggtgg ggctgggaaa
    27421 gctatcgctg tccagctcgc ttttgacggc gccaaagagg ttcgtttgtt taaccgacaa
    27481 gctacacgtc taagtagcgt tcaaaagctt gtgacaaaac tgaaccagct aacaaggact
    27541 aaagtaaccc ttcaagactt ggaagatcag actgccttta aggaggccat cagggaatct
    27601 catctattta ttgatgctac aagcgtagga atgaagcctt tagaaaatct aagtttgatt
    27661 accgaccccg agctcattcg cccagacttg gtggtctttg acatcgtgta cagcccagca
    27721 gaaaccaagt tactagcctt tgcccgtcaa catggcgccc aaaaagtcat taatgggcta
    27781 gggatggtgc tttatcaagg agcagaagct tttaaattaa tcactggtca agacatgcca
    27841 gttgacgcta ttaaacctct tttaggtgat gagtaacact ttttaagtaa gcttaattat
    27901 gatatattaa taagagatgg agcagtcatc atgggctgct aaaattcttt ttgcgagagc
    27961 tgtttttaag agcaaaaagc ttatttgaag agccttatcc ctaacgtgat acaagtcata
    28021 gttcctttga ggtctgccag ataacctacc gtttttttag aaaagaagct cttataaaag
    28081 gaaaagttca atgaataaag gagaatagca atggtaacaa aggaagttgg gcatgacttt
    28141 ttagcacgtc taggaaaaaa acgcttaaga cctggcggtg ttgaggcgac aaactggttg
    28201 attgaacaag gacaattctc tcaggattgt cacgttttag aggttgcctg caatcgctgc
    28261 acgacagcaa tagagttagc caaaaccttc ggttgtcaga ttgatgccgt tgacttaaac
    28321 cccaaggttg tcaaagaagc acaagaaagg gttgctgttg aggggttaac ccgtcacatt
    28381 acagtatccc aagccaatgc tttatcacta ccatttccag ataaccattt tgatattgtg
    28441 attaatgaag ccatgttaac catgttgaac gcccaagcca aggagaaggc actcaatgaa
    28501 tattggcgtg tgttaaaacc aggcgggcga cttttgacac atgacgtagc ttatgaagat
    28561 ccaaaaacaa gacaagtact tgacgagtta cgtcaaacga ttcatgtcaa tgttgctccc
    28621 ttgtcactaa caggttggca agccttatat caagaacacg gtttttctca agtgacttat
    28681 caccatggaa aaatgtcttt gatgacacct cgtggaatga ttaaggatga aggtcttatg
    28741 aacaccctga acattatgag acgaggcctt aagaaagaga acagacaaca gtttttaaag
    28801 atgcgtcgtt tttttaccag aacaggcaaa aatctctgct atattgctgt aaaaagtgta
    28861 aaataaacct tagaccaacc tgtccataca actaaacaga ccatagagga gttcgaagat
    28921 gtcttatatc aataatattg agcacgcgaa ggttcttgat ttgacgcaag aagtaatgat
    28981 tgagcaagat caaatgttaa gtagaacact agtccaaagg caagacctag gaataacagt
    29041 tttttcctta gataagggcc aagaaattgg gcgacactca tcaccaggag atgccatggt
    29101 tactatctta agtggcttag cagaaatcac cattgatcaa gagacctacc gggttgctga
    29161 aggtcaaacc attgtgatgc cagctgggat tcctcatgcc ttatatgcag tagaagcctt
    29221 tcaaatgctt ttagtagtag taaaaccgga ggtttaacat gattgaaaat ctctctcaac
    29281 tggtgcaata tcatgatcat caggtggcta gccgatcctt gagccgtcta ctgggattat
    29341 ctcaatctat tgttttatat gccatggccc aaggagaaag cattagtcaa gagactagtc
    29401 caaaagataa agtaatactt gttttagaag gtcagctcat tttcgattta gaggatcaaa
    29461 aacaggtctt aactcaagaa agtctcatag ccatacctgc ccaaaaagtc catcatttag
    29521 aagctaaaac cgattgtaaa ctattacaag tgcttttaga ttaagggctt attgtcaact
    29581 gtattgggtg gcctatggaa aagcaaaaga gaaggtgacg ctcatcttct ctttttacgt
    29641 attcaaagcg atgacaatac gaattttaaa gcttggcttt ctcatctcca gattaagccc
    29701 tttagcttgg tttattttag aattcactaa aaaaacctta atgagtttcc tatctcctta
    29761 taggtttctc attaaggctt tttagacgaa ataattgaac gacaggcaaa agaaagatct
    29821 ttttacgagt gataccgttc tcctatagtt ccataaatcc tctttttgat gttgctgtta
    29881 gttattctta gctaaaagtt atattgttat caagacttgt cttgtagtaa catgttttag
    29941 gagttccagg ataccgttat tctcttagca agcttaacta gctagagtgg agtcactatt
    30001 tataataaac tgtcgagctt ctcgacaaat tggtgatcgt ctttgttaat ggctagggtg
    30061 gtaaaaccgg tttctgtggg taatataaag ataatatgtt gctcagaatt cttcttatct
    30121 gttttaaaga cgtcaaagag ggatgtcgca gagacatcag ctctttcaag tgttgttggt
    30181 aactggtaac gttcaaaaac ttgaaggagg ctatccaagt cctcttttgg caagagtcct
    30241 ttggaaacag ccagtttagc atcaaccacc ataccgatag caatggcttc accgtgatgg
    30301 taaaaatcat ggtggcagag cgattcaatc gcgtggccca aggtatgccc aaaatttaaa
    30361 gacatgcgca aaccattttc aaattcatct tgttcaacaa tctgtttttt attggaaatg
    30421 gattggtaaa tcagacgttc aagtccctgc ttatcggttt ctttttgaaa atcacgtaac
    30481 tgctgaaaaa gcgatctatc atgaatgaag ccgattttga gcatctcact gatgccgcag
    30541 gagaattccc gttgcggtaa ggtctccaaa aaggtggttg aaataatgat agcttctgga
    30601 gggtaaatgc tgccaatcat attggttaga cctttaaaat ggactccaac tttgcccccg
    30661 atactgctat ccacctgact aagtaaagtc gttgggattt gaatgtaggg aataccacgg
    30721 taataggttg cagcgacaaa accacccaga tccccaacaa caccaccacc aatagtaaca
    30781 atcatatctt ttttggaaaa attttcagcg atgagctggt cgtagatagc actgacacgt
    30841 tccaaggatt tggtttgacc tcccgggggg cagacatgga caaaagcgtt gtattgctga
    30901 gcaaattctt caaagagagt ctgatacaag tggtagaccg tttgatcggt gataaacaag
    30961 agtttacgct gcttgcgctc accaaggcaa tctgctaagg tttttaaaac atggtcggta
    31021 aacaggatat cataatcctt gacccgagaa tggacatgaa gtgtttgtgg cataagcact
    31081 cctttcatca gtactaggta acagagataa cgcagtgata tttagagctt aatcttgcca
    31141 aagttcctgc cccaactgtt ctaattgctt ataatcaatt tgctgggcag catctgacag
    31201 cgcatggtcg ggatcaggat gaacctccat catgatgcca ttggcaccga cagccttcgc
    31261 tatcttagca gcaggtaaga gtaaatctcg tctgcctgtc gaatgagata cgtccacaat
    31321 aatcggtaaa tctgtctttt gttgaataat agggactgcc ataatatcaa gcatatttct
    31381 agtctccaca tcgtagccac gaacgccacg ttcgcataag ataatgttag attttccagt
    31441 gtcttgtaag taggaaaggg cacccaaata ttcctcgata gtagccatca agcctcgttt
    31501 aaacaaaata ggcttatcga tgtgtgacaa ggtttttaaa aattcaaaat tttgcatgtt
    31561 gcgggcacca acctgaatga catcgagata gtcataggct tcttctaatt ggcgctcgga
    31621 catgatttca ctgaccgata gtaagccaaa ttcctgacaa acctcatgca agtaacgaat
    31681 tccttgcaga ccaaggcctt gaaaagaagc agcagatgtt cttggtttat aggcgcctcc
    31741 tctaaaatag ttgtaaccaa gttttttggc acttgaagct gctaatctaa tgtgatcata
    31801 agattcaatg gaacaaggtc caacaataaa attattctta gaacaagtct tattttgaaa
    31861 gtcagacata atgtcaatca ttcgtttgat tcctttacta attctgtatg gcgtgctcta
    31921 tgggataacg tcttatttag gaatacaaaa tctcgttgtt atccatgaag cacccttagc
    31981 ctgtgaaatg ataccacaaa caagatgaaa tagcaagtaa accctagatt gtctatgttc
    32041 ttagtcattt ttattaaaaa aggcttgtta gctataaaaa aatactgccc taaatcttat
    32101 taggacagta ttttttatag atgccaccac cgagaatcga actcggaacg aagcattacc
    32161 attgctttat tataccattt aactacagcg gctacctttt tataatacta taagttatta
    32221 attgatgcaa gcttttctta aaaatatttc cttaattgtt tatcatcaaa atgagagtac
    32281 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agatgatttc
    32341 agaaagagga agctaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
    32401 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
    32461 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
    32521 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
    32581 ccagatgagt tatttggagc aaggcattat taagaaggag acacgaaata gtggcaaaac
    32641 aaaaaaatcg taaaaaagca ttgaagttag aaatgaagcg taatggcttg ttaaaaaaag
    32701 caggtcaagt ttttgataag gcagttgaaa gtgttgaaac agctgtggat aaaaccattt
    32761 ctgctggtaa aaatcttgtg gaaaaaggca gccaaacggt tgaaaacctg acagcaagca
    32821 aggaaagact tactcttgat gagtttgcag ggcagccagt tgtagctggt atccgctctg
    32881 accttgtcga aaccctatat gcagaaggca tccattcagc gcaagctttt aaggagtgga
    32941 cagaaaaaga cctccttgct cttaagggca ttggcccagc aacggttaaa aaattagtcg
    33001 aaaatggcgc aagccttaaa aaatagtcat cttctcgttg cttaatggct ataaatttgt
    33061 tagaatatta gcaatcagag gtgcattgag ttggtctgtt acagagagcg gtggtgttga
    33121 gaaatccgca cagatgtcaa ggctagtagc tgaggatcag aaatcagata tgaaaagtag
    33181 cagacccatt ttatggtctg aaacagtggt ggtaccgcgg aaatcgtaac acgaaattcg
    33241 tccctgtcta ggttatctag gcagggatta tttggtttca tttgaaagaa gatgatggct
    33301 tattgttaaa atgttgttag aagtatataa tagaaaaact ttgcccaaag tggaaacaga
    33361 acggctgcta ttacgtcaac gaaaattgga ggacgccgag gccatatttg cctttgctaa
    33421 actggctgag gtcagttatc ctgctggttt tccgcctgtt aaaagcttag cagatgaaat
    33481 tgcctatctt caagacattt acccagccaa tcttgaaaaa gaaggactgc cttctggtta
    33541 tgccattact ttaaaagaca atgacaatgt tattggttct gtggatttta acccagaaga
    33601 accacccaaa acgctcacgc attaccaaaa aatcaataat acccagctca ccgctgctga
    33661 aacagcgcag cttattatcc aaaaaatgac tcatatcaag gagaactaag atgacagaac
    33721 tatcacctaa atacaatcct gccgaggttg aggcaggtcg ctatcaaaaa tggcttgatg
    33781 cagatgtttt caagccatca ggagaccaaa aagcaaaacc gtattctatc gtgattccac
    33841 cgccaaatgt gactggtaag ttgcacttag gacacgcctg ggatacgacg cttcaagata
    33901 ttatcattcg tcaaaaacgc atgcagggct ttgatacgct ttggcttcca gggatggacc
    33961 atgcaggaat tgcgacacag gctaaggtag aggagcgtct gcgcgagcag gggatttctc
    34021 gttacgacct tggtcgtgac aaattcctag acaaggtctg ggagtggaaa gacgaatatg
    34081 ccactacgat taaagagcag tggggcaaaa tggggctttc tgtggactac agccgtgagc
    34141 gcttcaccct tgatgagggc ttgtcaaaag cagttcgcaa ggtttttgtt gacctctaca
    34201 aaaaaggctg gatttaccgt ggtgaattca tcatcaactg ggatcctgct gccagaactg
    34261 ccctttctga catcgaggtt atccacaagg atgttgaggg agctttctat cacatgaact
    34321 atatgttgga agatggctca cgcgcccttc aagtggcaac aacgcgacca gaaaccatgt
    34381 ttggagacgt tgccgttgcc gtaaacccag aagatcctcg ttacaaagat ttgattggca
    34441 aaaatgttat tttaccaatc gtgaataaat tgattccaat cgtgggtgac gagcatgctg
    34501 accctgaatt tgggactgga gttgtgaaaa tcacgcctgc ccatgatcct aacgatttcg
    34561 aggttggtca acgccataat ctaccacaag tgaatgtcat gaacgatgac ggtaccatga
    34621 atgagttagc tggcgacttt gcaggcatgg accgttttga agctcgtcaa gctaccgttg
    34681 ctaaacttga agaacttggt gccctagtaa acatcgaaaa acgtgttcac tcagtaggtc
    34741 actcagaacg ttctggtgca gtcgttgagc ctcgtctatc cacccaatgg tttgtaaaaa
    34801 tggatgagct tgctaaacaa gctatggaca accaagaaac agataaccga gtagatttct
    34861 acccaccacg ctttaatgat accttccttc aatggatgga aaatgtccat gactgggtta
    34921 tctcgcgtca gctttggtgg gggcaccaaa ttcctgcttg gtacaatgca gaaggtgaga
    34981 tttatgttgg cgaagaagca ccagaaggtg atggctggac tcaagatgaa gatgttcttg
    35041 acacttggtt ctcatctgct ttatggccgt tctcaactat ggggtggcct gacacagatg
    35101 tagaagattt caaacgttat ttcccaacct caaccttagt aacaggttat gacatcattt
    35161 tcttctgggt gtcacgtatg attttccaat cgcttgaatt taccgggcgt cagccattcc
    35221 aaaatgtcct aattcacggt cttattcgtg acgaagaagg acgcaaaatg tctaaatcat
    35281 tggggaacgg gattgatccg atggatgtca ttgagaaata tggtgccgat agcttacgtt
    35341 ggttcctctc aaacggctct gccccaggac aagacgtgcg tttctcatac gaaaaaatgg
    35401 acgcctcttg gaatttcatt aataaaattt ggaatatttc tcgctatatc ctcatgaata
    35461 atgaagggtt gaccttggaa gaagctgaaa gtaatgtggc taaggtagct gcttcagaag
    35521 ctggaaatgt gactgaccag tggattctcc acaatctcaa tgaaaccatc gctaaagtga
    35581 cagaaaactt tgacaagttt gagtttggtg tggcaggaca cattctttac aacttcatct
    35641 gggaagaatt tgccaactgg tatgtggaat tgaccaagga agtgctgtat agcgacaacg
    35701 aagctgaaaa agttatcacg cgctctgtcc ttctttatac tttggacaag attttacgtc
    35761 tccttcaccc aatcatgcca tttgtaacag aagaaattta cgctcaatat gcccaaggtt
    35821 ctattgtgac ggtggactat cctgtggttc gcccagcttt tgaaaatgaa gctgcgcata
    35881 aaggtgtgga aagtctgaaa gatcttatcc gtgctgtacg aaatgcgcgc gctgaagtta
    35941 acgtggcacc atcaaaacca atcaccattt tggtgaagac agcagatagt gaattagaag
    36001 acttcttcaa cagcaatatc aactacatca aacgcttcac caatcctgaa aaattagaaa
    36061 tcagctcagc cattgctgcg ccagaactgg ctatgaccag catcatcaca ggtgctgaaa
    36121 tctacttgcc actagcagat ttgcttaacg tggaagaaga actcgctcgc ctagataaag
    36181 aactggctaa atggcaaaaa gaactggaca tggtcggcaa aaaactcggc aacgaacgct
    36241 ttgttgccaa cgccaaacca gaagttgtcc aaaaagaaaa agacaaacaa gccgactacc
    36301 aagccaaata cgacgcaacc caagaacgga ttgcagagat gcagaaaatt aaatcatagt
    36361 gtttagaacg cagttgaaaa actgtgttct tttcttacta atagagatat tagattgaag
    36421 tttaattatg agaaaaacgc tttctttatt taaagagtta tgttacaata ctattacaat
    36481 catcgtacaa atagtgttat tttgaggggc atatgagaat gattttagca cactatgact
    36541 gtaaaaaaga taaaaagcaa tctttagatg agcatttatg gcatgtggcc tgttctagtc
    36601 gacaggaagc atctataatt ggtcaaggag atgtgctttt tttaattggt ctttaccacg
    36661 acctgggcaa agctgatcga acctttcaag ataaattatt aaataatcca aatcggcatg
    36721 ttgatcactc ttatgcaggg gcaaaatact tatgttctat tattgggcct catctaaaaa
    36781 accgaggggt tgataaaaat gagagaatga cattcaacga aatggtgggg tatgtcatct
    36841 ctgctcatca tgggatgtat gatttatgct actattttga cgatgctgaa tattatggct
    36901 ttaataagtt taaaaatcgt atcaatagag acttagatgg ttatcactat catgaagata
    36961 ttaaagggta cgctctaaaa ttagaaaaaa aattatgtga ttatggctac aaagatttaa
    37021 gggagcttat tgataaagct tttgataatt accaacaatc catgtcttcc ttaaactggc
    37081 aagataagag tgagtgggat tattatcagt cttgtatggt gagactttac ttgtcactct
    37141 taaaaaacgc tgatattttg gacacagtaa atgcctatgg ccttaagata agtcctatgg
    37201 ataaaacaga gcgatccttt ctaaaacact cctatttagc ggccattgaa caaaaatatg
    37261 ctagctttgg acagccaaac aatcagttga acactattcg gacagaaatc gctgagcgtg
    37321 ttaaagaaag aggtaaacga gattccaagg ggatttatcg cttagattta ccgacaggag
    37381 ctggcaagac taatcttagt atgcgttatg cgtttcacca attagttcat cacgacaaat
    37441 caaggttttt ttacataacg ccctttcttt cggttcttga gcaaaatgct tccgaaatta
    37501 gaaaagttac aggtgacctt ggcgttctag aacaccattc caatgtggtg aaacaggcta
    37561 atgaagatga tgatgacaag gacagtttat tgtcagctta tcttagtgat agctgggaca
    37621 gtcaagtagt cttgacttct atggttcaat ttttccaaac acttttcaaa acaaaatcag
    37681 ctaatctgag acgtttttca agtttgatta atagtgttgt gattctagat gaagttcaat
    37741 ccctgcctat tgaagtcacc actttgttta atttaacgat gaatttttta aataaagtta
    37801 tggatacaac catcgttctt tgcacagcaa cacaacctgc ttatgattct tcagagattg
    37861 accatcgtat ctgttatgga gggaacttgg gagaattagc tgaaatcgtt gagttaacga
    37921 ttgaagaaaa acagattttt tcaaggacag agcttagaaa atttgatgat agtgatcaga
    37981 aagttcactt gactgatgtt attaacctta ttctaggtga ggaaaactca gttcttgcta
    38041 tttttaatac gaaaaaaacg gttcataact gctatactat gctaaaagac atgactgata
    38101 gaccggtcta tcagctttcg acaaatatgt gtgcgcagca tagacttgac ttgattgcta
    38161 agatcgaaac ggagttacaa aataatatcc ctattatttg tattagcacg caattaattg
    38221 aagcaggtgt agatgttgat tttcatcgcg tcattcgttc ctactcaggg attgattcta
    38281 ttgttcaggc tgctggacgg tgtaaccgag aaggcaaacg agataaaggg caagtcactc
    38341 ttgtcaatct gaccaatgaa gaggaaaata tttctaggct gacagaaata aaagctaaaa
    38401 aagaagccac agaatctatt cttcataaga ttgggtctcc aattgatatc tcaactttaa
    38461 accgtgactt ttttgagtat tattatgcca ataatcaggg actgatggat tatcctttgg
    38521 aagacaacct atcaatctac gactatttaa gccttaatat ttatcagacg gcaaataaaa
    38581 agttcaaagg taagttaaaa caagctttta aaacagcagg agccaaaatg aacctcatca
    38641 ataatgatat gataggaatt ctcgtacctt atggcgaagc tgagaaaaaa ttggcttatt
    38701 tagaagaatt gggtatgtct cactttttat cagcagaaga ttatcaaacg ataaaatcat
    38761 tactaaaaga gttacaacct tttacggtta atgtccgcga gaacgatcct ctctttgagg
    38821 caacaaaatc ttatctaaat ggtcagattc tggttttgac gtcggagtat tatgacacgg
    38881 aaagaggagt taaatacgat tcagctagct tttacttcta actcaaaacg aaagcagatt
    38941 aacaaaaggt tgttagagga ccttgttaac ctgccaatca tcattaataa ttattatcaa
    39001 tttagactat ttaataaaat tagattacaa aaaaacagaa ggaggaaagt agcttgtaca
    39061 gatctagaga cttctacgtg agagtaagtg gtcagcgagc tctttttaca aatccagcca
    39121 caaaaggggg atcggaacgg tcatcctatt cggttccgac tagacaggca ctgaatggta
    39181 tcgttgatgc catctattat aagccgacct ttactaatat cgtcacagag gttaaggtta
    39241 ttaaccagat tcaaaccgaa ttacagggtg tcagggctct gttacatgat tatagtgcag
    39301 atttaagtta tgtatcctat ttgagtgatg ttgtttatct gatcaagttt cattttgttt
    39361 ggaatgaaga tagaaaagat ttgaactcag atagactttc agctaaacat gaagccatta
    39421 tggagcgttc tattcgtaaa gggggacgtc gagatgtgtt tttgggtaca agagaatgtt
    39481 tagggcttgt agatgatatc agccaagaag agtatgagac tactgtgtcg tattataatg
    39541 gtatcaatat cgacttggga atcatgttcc attcctttgc ctatccgaag gacaaaaaga
    39601 caccattaaa atcatacttt acaaagactg tgatgaaaaa tggagtcatt acgtttaaag
    39661 cacagtctga atgcgatatt gttaacacgc tttctagtta tgcttttaaa gcaccagagg
    39721 agataaaatc ggttaacgat gaatgcatgg agtatgatgc catggagaaa ggagaaaact
    39781 gatggatttt tttacttctc tcttgaagac ttatgaaaaa gcagagctag cagacttggt
    39841 tgatcatcaa aaaagaaata atgagccggt tttactgccg atttatcata cgagtttaaa
    39901 gtcaaatggc aaaaatatca tttcagtgaa acttgacaaa gatggccagt ttcacaaggc
    39961 agaatttatg gcagataagc aaatgattat ttttcctgta acggctgatt ctgttgctag
    40021 gtcaggtagt catcctgcac cgcatcccct agtcgataaa ttttcttatt atagtgctga
    40081 aatggggcag actcagtatg attcttttca taagcaactg aataactgga ttgattattg
    40141 tgaggagggt gacgtcaaga ggtttttaac cattgttcag caatttattt tgaaaccaga
    40201 atttctgaca ctgattctcg attctttaat tggtcctgat tatcaacata atcaattaaa
    40261 agtcacattt tgtgatgcca ctggaaaaga aaaattaatt gatttatcag cttgcttttt
    40321 agaattttca attgatcagt tccagggctt taaaaatgaa tcggtttcga cgtttaaagc
    40381 tttacaccaa tcctatattt cttttgttga agccaatcgt gaaaatctcg gtatttgtaa
    40441 tattagtgga cgagaggaac agcttaccga taagcataga ggtttgatgg ggaatgctaa
    40501 aatcatctct gttagtaata aaagagaagc ttataaagga cgttttagag aacgcgaaga
    40561 cgtttttagt gttggctatg aaacttccga aaagattcat ttaatgatca agtatctttt
    40621 agaaaataaa aataccagta cttggttagg gtcctctcaa tatttaatca actggttcag
    40681 cgatgattta acaaatgata gtcggttgga tattgtatca ccaatttttg atgatggact
    40741 tgaagaagat gatgatgacg atacgcctcc tgttataaca ttagcaactg aagacaataa
    40801 aagaattggt aaatcattca tcaagggaca aaaattattt gctaatgatg ccacttacta
    40861 cgttgctatt ttgaataaaa ctagcaatgg gcggattgct ttaaaatatt ttcgtcagct
    40921 tcaagcgtcc caattactca ccaatcttaa caagtggcag gaaacataca gttgggagtc
    40981 gcgatctaag tttggaaaaa gtcgcttaag aacccctact tttcatgaca tccttaatgt
    41041 gtcctacggg gttgataggg atcgcttcct tgaattagat aatgataact tcaaaagtga
    41101 tcaaattcaa aagttagtgg caagtttgat tgatggtaaa ccgatgccac agtccattgt
    41161 caaaaagtta ggtaacaatg ttaaagaacg acatcgttac cgtaagcact ggtatcaagt
    41221 tgagcaggtc tgcttagcaa ttttacacaa acaaaatggg gaggaatttt caccgatgct
    41281 agatcatacc aatcaaaatc gttcctatct ttttggacga ttattagcaa tttttgaatt
    41341 aatcgagacc ttgcgttatg gcttggatgg aaacaataac gatcgtatta ccaatgctga
    41401 acgttattgg acagcctata ctggacaacc aacaaaattg atgatgttat tggaaaataa
    41461 aattaagcct tacgaagaac cattgaaatt aaatcgtcgt ggcagttgga tgaaattaga
    41521 aaaagaaaaa gaagagattt tagaactgtt aaatcctctg ttagaaacag aaacaatgga
    41581 aaaaccctta gattaccgct ttatttttgg gtattatgct gagaaaaact attactatac
    41641 aaaacaaaac acggaagtaa cagaaagtga ggagtaaaaa gatgttggaa cacaaaattg
    41701 attttatggt aactcttgaa gtgaaagaag caaatgcaaa tggtgatccc ttaaatggaa
    41761 acatgcctcg tacagatgcc aaaggatatg gtgtgatgag tgatgtctcc attaaacgta
    41821 agattcgtaa tcgtttgcaa gatatgggga agtctatttt tgtgcaagct aatgagcgta
    41881 ttgaagatga ttttcgttca ctggaaaaac gcttttcgca acattttaca gctaagacac
    41941 ctgacaaaga aattgaagaa aaagcaaatg cattatggtt tgatgttcgt gcttttggac
    42001 aagtttttac ttatctgaaa aaatcaattg gggtgcgtgg accagtttcc atcagtatgg
    42061 ctaagtcctt ggagccaatt gtcatttcca gccttcaaat tacgcgtagt accaatggta
    42121 tggaagctaa gaataatagt ggccgctctt ctgatacgat ggggacaaaa cattttgtag
    42181 attatggtgt gtatgtactt aaaggttcta tcaatgctta ttttgctgaa aagactggtt
    42241 tttctcagga agatgctgag gctattaaag aagttctggt tagcttgttt gaaaatgatg
    42301 cgtcgtctgc acgtccggaa ggctctatgc gagtttgtga agtcttttgg tttacgcatt
    42361 caagcaaact gggaaatgtt tcaagtgcgc gtgtctttga cttgttagag tataatcaat
    42421 caatagaaga aaaaagcact tatgacgctt atcagattca tctaaatcaa gaaaaattgg
    42481 ctaaatacga agcgaaaggg ttaacggttg aaatcctaga aggactctag tatggtctat
    42541 gccgaagatg attatttaat gctatcaggt attcagcatt tccaattttg taaacgtcaa
    42601 tgggcgttga tccatattga gcaacaatgg cttgataatg aagcgacagc gcatggacag
    42661 gttttacata ctaaagcaga taacccttac attaaagaaa aacgaaaaga gcttttggtc
    42721 tcacgtgcta tgcccatttc ttctgcagaa cttggacttt caggaattat ggatgttgtg
    42781 gaattttata aagatgatca aggtgtgtct ttgaggggaa aacgtgggaa atggttacca
    42841 aaagttgtgg aatacaagcg cggaaaacct aaaaaagata ccagagatat tgtccagttg
    42901 gtggctcaga ccatgtgttt agaagaaacg ctagactgcg acattaacga aggttgtctt
    42961 tattaccata gtgtcaatca aagagtgatt gttcctatga catcagcttt gcgtcaagaa
    43021 gtgaaggaat tagccgcaga gatgcatgag gtttatcaga gtcaaatgct acctaaagca
    43081 gcttatttta aaaactgtca gctttgttct ttagtcgata tttgtaagcc caggttgagt
    43141 aaaaaaacaa ggagtgtgtc gcgttacatc aatgaggcta tgaccagtga ggagatggac
    43201 ctatgaagaa gttgctaaat accttgtatt tgacgcaaga agatttttat gtcactaaag
    43261 agggcgataa cattgttatc aagcaagaag gtaaggttct caaacggttt ccgtttcgga
    43321 ttattgacgg tattgtctgt ttttcttatt tgggtgtgtc gcctgctttg gtgaagttat
    43381 gtacggagaa tcagattaat ttatcgtttc atacaccaca agggcgtttt tgtggtcgct
    43441 atattggttc aaccaatggg aatgtgttgt tgcgtagaga acattatcgt ttatctgatc
    43501 gtgaggaatc tttggaatac gcaaagcggt ttattttggc taaaatttcc aattcaagga
    43561 aatacttgct acgctttaaa cgagatcatc gtcaacagat tgataccaag ctttttgagg
    43621 ctgttaatga cgaattgata tgggctttag agatggttca ggcagcagat aataaagact
    43681 ctttaagagg gattgaaggc caagctgcta atcagtattt tcgcatattt aatgacctgg
    43741 tgttgacgga caaaaaaacg ttttacttcc aaggtcggag taaacgacca cccttagatt
    43801 gtgttaatgc cctcttgtct tttggttaca gtttactgac ctttgaatgt caatctgcct
    43861 tggaagctgt cggattagac agttacgttg gtttctttca cacggatcgt cctgggcgtg
    43921 ctagtttagc gcttgattta gttgaagagt tccgctcata tattgtagat cgttttgtct
    43981 tttcattaat taataaagga caacttcaga aaaaacactt tgaggttaaa gaaaatggca
    44041 gagctatttt tattgatttg tggcagaagc gtaagcatac tgaggtagaa catcctttta
    44101 caaaagagaa agtaaaactt atgttattac cctatgtaca agcgcagctt ttagctaagg
    44161 ccatacgagg agatttagaa agctatccac cttttatggt ttaggagatg ttatatgatg
    44221 gttttagtca cttatgatgt aaatacggaa acacctgctg gtagaaaaag attgcgtcat
    44281 gttgccaaac tctgtgtgga ctatgggcaa cgtgttcaaa attctgtttt tgaatgttct
    44341 gtgacacccg cagaatttgt ggatataaag caccgcttaa cacaaatcat tgatgagaaa
    44401 actgatagta ttcgctttta tttattgggg aaaaattggc agaggcgtgt ggaaacactt
    44461 ggtcgctcag acagctatga cccagataaa ggtgtcttat tattgtaaaa atctcttgtg
    44521 cgaagctagc tttcacagaa aaaccttgct tgctcgcgca aaaataactt aaaaaagaag
    44581 cgaaatggag ataaaaaggc ttaaaacacc tctatgtgtg ccatccgttt ctctataaac
    44641 tgtgtcattt ggcgcagtct cacccttcat gggtgagtgg attgaaattt aaatttagta
    44701 tctaattttg attttgcatc ctcgtctcac ccttcatggg tgagtggatt gaaattatct
    44761 gatttttaaa tctgaaataa cttttaaagt ctcacccttc atgggtgagt ggattgaaat
    44821 acactcttct aaaaagttta aaaagtcaat aaagtctcac ccttcatggg tgagtggatt
    44881 gaaatatcat caatagactt gacttgtaac aatatctgcg tctcaccctt catgggtgag
    44941 tggattgaaa tgttttaaaa cggattataa tataatcaag cttaagtctc acccttcatg
    45001 ggtgagtgga ttgaaatacc tctcataata tctttaagtt ctcttacctc acgtctcacc
    45061 cttcatgggt gagtggattg aaattcctct catcatatct tttaaatctt taacatcgtc
    45121 tcacccttca tgggtgagtg gattgaaaga tgaatccgac ctctctttta atgaggtcgg
    45181 gtttttacat gcctttagtc aaccatttat ctgatagcgt atcaaattca agtttttgca
    45241 tgttctagtc tatggtttgc gccactagct ttcggtaaac taagggtaac ttaagagagg
    45301 agttatttat gacactatca cttgtattaa tggtatcggt ttttggggca ggcttgttat
    45361 cctttttctc tccgtgtatc tttccggtcc ttcctgttta tttaggtatt ttactggatg
    45421 cagatgattc aaagacgatt actatatttg gtaaaaaact ctattggtat ggcattgtca
    45481 agaccttagc cttcattttt gggttatcca ctatctttgt gattttaggt tatggggctg
    45541 gttttttggg aaatatcctt tatgcggtat ggtttcggtc cttcctggga gccttggtca
    45601 ttatcttggg gattcatcag atgggcctca ttaccattaa gagcttacaa tttcaaaaat
    45661 cactgacttt ccataataac aagaatcgca acggtttgtt caatgcattt atccttggtt
    45721 taacctttag ctttggttgg acgccttgtg tgggacctgt tttgagttct gtcctagctt
    45781 tggtggcttc agggggaaat ggtgcctggc aaggtggcgt tttgatgatt atttatactc
    45841 ttggattggg cattcctttc ctacttatct cttttgcgtc aggcattgtt ttgaaacagt
    45901 ttaacaagct caaaccccac atacttttac tgaaaaaagt aggaggcgtt ctgattatag
    45961 tcatgggaat cttgcttatg acaggaacct taaataactt agcacaactt tttggataaa
    46021 ggagaaacac aatgaaaaaa ggactattag taacaactgg tttggcttgt ctcgggctac
    46081 taactgcttg ctcaacccaa gacaatatgg ctaaaaagga aataactcag gacaagatga
    46141 gcatggcagc taaaaagaaa gataagatgt caacatcaaa agacaagtcc atgatggcag
    46201 ataaatcatc tgataagaaa atgaccaatg atggtcctat ggcgcctgat ttcgaactca
    46261 aaggcattga tggtaagacc tatcgcttat cagagttcaa aggtaaaaaa gtttatttga
    46321 aattttgggc ctcttggtgt tcaatctgtc tatcaacctt ggcagatacc gaagacctgg
    46381 ctaaaatgtc agataaagac tatgtggtct taaccgtcgt ctcgccaggt catcaaggag
    46441 aaaaatcaga agcagacttt aaaaaatggt ttcaaggaac agactataag ggcttaccag
    46501 tcttgttaga tccggatggc aagctattgg aagcttacgg cgtcagatct tacccaacag
    46561 aagttttcat tggaagtgat ggtgttcttg ctaaaaaaca cattggttat gccaaaaaat
    46621 cagacatcaa aaagaccctt aaaggtatac attaggaagg accagctgat aggcattaga
    46681 aaaaaggagt tgagtttatg gaacacaaat ggaaattatg gctatttatc gttggagtgg
    46741 tcttgttaat aggcggagct tctgttctca ttcagaaagg tcaagccaag gcttttagtc
    46801 aaggcaagcc tcaactacca gctaaaaaag tcaaatccac taaagtagat cccaatgtcc
    46861 ctgttgatca aaaagtgatt tacctagctg gtggctgttt ctggggcgtt gaagaatatt
    46921 tttcacaagt gaatggcgtt ctagatgcgg tgtcaggata tgccaatggt agaggcgata
    46981 ccaccaacta ccagttaatt catcagactg gtcatgcaga aaccgttgaa gtgacctatg
    47041 atgcgaaccg tattagcttg aaagaattat tactgcattt tttccgaatt attgacccaa
    47101 ccagtcttaa taagcaagga aatgatcgcg gtagtcaata ccgaactggg atttactata
    47161 ctgacaaagc agatttagct attattgatg aggtcttcaa agaaaaagct aaggattaca
    47221 agaaaaaaat agtggttgaa aaagcaccat tgaaacactt tattaaggca gaagactacc
    47281 accaagatta cctcaagaaa aatccaaatg gctactgcca tattgacatc aatcaagcga
    47341 cttatcctgt gattgatgaa agcaagtacc caaaaccaag tgccactgag atcaaagcaa
    47401 aactatctgc agacgaatac cgagtgacgc aaaaaaacga aactgaaaaa gccttttcta
    47461 atcgctactg ggattctttt gatgcaggaa tttacgtaga cgttgtgaca ggagaaccgc
    47521 ttttctcttc aaaagacaaa tttgaatcag gttgtggttg gccaagtttt agccgcccca
    47581 tcagcccaga tgttattcgt tacaaggaag ataagagttt caatatgacg agaacagagg
    47641 tgcgcagccg ttctggcaat tctcatctgg ggcatgtctt tactgatgga cctaaagacc
    47701 aaggtggcct tcgttactgc atcaatagct tgtctattac ctttattcca aaagctgata
    47761 tggaagctaa gggctatggt tatttattat catctgtaga atagggttag gcctaaaaat
    47821 ctgatataat agaagaaaga ctagtatgag agggtcatgc aaggcccttt ataaagataa
    47881 ggagacaccg tgtgtactca ttgttaattg tagaagacga ataccttgtg cgccagggta
    47941 ttcgttcttt ggttgatttt agccagttca agattgatcg ggtcaacgaa gcagaaaatg
    48001 gccagttggc ttgggacttg tttcagaaag agccttatga tattgttttg acggatatca
    48061 atatgcccaa attaaatggg attcaactag cagaactcat taaacaggaa tccccccaaa
    48121 ctcatctggt atttttgacg ggctacgatg attttaacta tgccttatct gctttgaagt
    48181 taggggcaga tgattacttg ctcaaaccct tttccaaggc cgatgtggaa gatatgttag
    48241 gaaagctcca gcaaaaatta gacctttcca agaaaacaga aaccattcag gaattggttg
    48301 agcagcctca aaaagaagta tcagcaatag caatggctat tcatgagcgg ttggcagatt
    48361 ctgatttgac cctaaaaagc ttggctcagc agcttggttt tagccctaat taccttagcg
    48421 tcttgattaa aaaagagtta gggatgccct ttcaagatta tctggtacaa gaacggttga
    48481 aaaaagccaa gcttctcttg ttaaccagta atcttaaaat ctatgaaatt gcggagcaag
    48541 tgggctttga ggacatgaat tatttttctc agcgctttaa gcaactggta ggcgttaccc
    48601 caagtcagta taaaaaagga ggccaggcat gaagcgttat cccctactgg tccagttgat
    48661 ttcgtatgtc tttgtgatcg taattgccct tattaccaca ctgggcttac tctattacca
    48721 gaccagttct cgtaatatca ggcagttaat tgaacgtgat actcggcaga gcattcggca
    48781 aagttcccaa tttattgacg cttacatcaa gcctcttaaa gaaacaactt cggtgctggc
    48841 gaaaaattcg gaaattcaag actttgctag tcaaattcat caagaaaatg acaaacaggt
    48901 tcttcagctc atgaagatgg ttcttgcgac caattccgat ttacaagcag ctgttctggt
    48961 gactaaggat ggtcgaacgg tgtctaccaa ttctcagttg attatgaaaa cctccagtga
    49021 tatgatggca gaaccctggt ataaagcagc catagaccgt caagccatgc caatcttaac
    49081 cccagctcgg caattatccc tttcttctaa aaaagaatgg gtagtttctg tgacccaaga
    49141 ggtagtggat gctgctggtc ataatttagg cgtgctaaga cttgatattg catacccgac
    49201 tattaaagcg tctttagatc agcttcagct aggccgccaa ggctttgcct ttattgtgaa
    49261 tgataagcat gaatttgttt accatcccaa aaagagtgtt tacagttctt ctaaggagat
    49321 ggctgcgatg aagccttatt tagcgattca gaatggttac actaaggaca agacatcttt
    49381 tgtgtaccaa aaactcattc ctaacagtca atggacttta gtgggagtgg cgtcactgga
    49441 tcagttgcac cgggtgcagc gccaaatttt ttggtccttt cttggaacag ggcttctagc
    49501 ctgtctgatt tgtggctttg caactgtctt agccttacgc agatggattc gtcccattca
    49561 acaattgcag caagttattc ttgctattca aaaaggagat cgtcagttac gtgcccaaga
    49621 aacgggttct ccagaattga cagaccttgc ccaacagttt aatgcgctct tagatcaaat
    49681 tgatagcttg atggttgccg ttgcggataa ggaaaaggcg attgggcagt ataggttaca
    49741 agccttggct agtcagatta acccgcattt tctctataac accttggaca ctattatttg
    49801 gatggcagaa tttaatgaca gcaagcgcgt ggtagaagtg accaagtctc tagctaagta
    49861 ttttcgtttg gcccttaatc aggggaacga atacattcgt ttggcagatg aactggatca
    49921 cgttagccaa tacctcttta ttcaaaaaca gcgctatgga gacaagctaa gttatgaagt
    49981 gcaaggctta gatgtctacg cagactttgt tattcctaag cttatcttac agcccttagt
    50041 agaaaatgct atctaccatg gcatcaaaga agtcgatcgc aagggcatga tcaaggttac
    50101 ggtatctgat atagctcagc atctggtgtt gactgtttgg gataatggta aaggcattga
    50161 agactcttca ctgaccaata gtcagagctt gttggctagg ggaggtgtgg gccttaaaaa
    50221 tgttgaccag cggttaaaac ttcactatgg tgaaggctac cacatgacca ttcatagcca
    50281 gtcagaccac ttcactgaaa tacaattaac ccttcctaaa atgcatgaat taatggcaga
    50341 cgacacacag gaaaacgagt aaggttacag gaagtgcatg tcagccattt ccctaagtta
    50401 gggtattgga gataagaacc ttagaaagga tatcatgaat ttagcaactg actattttag
    50461 ccgacagacc cccatagttg aaaagctagt ggcttacggt tttgagaagc gagataacgg
    50521 ttatttctat aacgaacggt ttatggaagg agaatttgag gcgcagctta ggattgatga
    50581 ggctggcaat atctgggatc gggttattga ttgtgatttg gaggaggatt atttgccttt
    50641 acaacaagca gcctggcaag gaacctatac tgggcaggtt agagcagcct atttggaact
    50701 cttggagcgc ttatcagtag cctgttttga agtcactcct tttcagtcaa tgcaagctaa
    50761 tagacttgct aagcatatca ccaaagaatg gtctgaccca atggactatc cttttgaaaa
    50821 gcacccagac cttgcaacct atcgggtagg gggcaaatgg tatgctatga ttttttctct
    50881 cttggctgat aaattagacc agataccaaa gagattagta ggacagacat gtgaagtgat
    50941 gactgtcaaa gttaatccca aggctttccc tcagttactg cagcaagaag gcatttaccc
    51001 agcgtatcac atgtcaaaga gaaactggat tagtattatt ttggatgata aggtaacaga
    51061 tgatcagctt tggacgttag tgactcaaag tcgtcaacta gtcaacccaa acggtctttc
    51121 taatcctaat ggtcctgatt attgggttat tccagctaat ttgaaatact atgatattga
    51181 tgcagaattt gcagccaatg atgtgatttt atggacccaa aaagctggta ttgccgtggg
    51241 agactacgtg ctgatttata taacagcccc agtcaaatcc attcgctatg tctgccaagt
    51301 tctagaaact gatataccta acgagggtta tcgtaaaaat cctaacattg acaaactcat
    51361 gcgtctccga aaacgccaac agtacaagga tggtctcttg tcttttgacc ttatgaaaga
    51421 acatggcgta gctgcagtga gagggccaag acgattaagc cctcaattaa ttgcattttt
    51481 aaaggaaaaa gagtatttta aagagaacaa ctaaaggaga ataagagatg gttgaatttg
    51541 tagcagatca aagtttttgg gaactttttc cagatgccaa actaggtgtc gtcttattaa
    51601 aggatgttca gaatcaagca gagtcaccag aagatgtcaa acagctctta gcagaaagcc
    51661 atgagttagc aaaagcttat ttaacagcag acaattttag tgataatcag gtggttcaag
    51721 tctaccgcaa ggcctatcaa cactttaaaa ccaaaaaagg agctcgctct agcattgaag
    51781 cccttctcaa gcgggtgtca aatggtcaaa gcattccgtc gatcaatccg ctagtggata
    51841 tttataatgc ggctagtcta cgttttggtt tacctgttgg agccgaagat agtgacagtt
    51901 ttatcggaga tttgcgtctg acgataacag atggtggtga cgacttttac ttgattgggg
    51961 atgctgataa caacccaacc ttgccaaatg agctgtgtta taaagatgat attggagcgg
    52021 tttgtcgttg ccttaactgg cgagatggcg aacgcaccat ggtgacagaa cacaccaaaa
    52081 atgcatttct gattatcgaa gctcttgatc aagaaggtca aaatagattg caagaagctc
    52141 ttgacttcat tgaaggcagc gctaaaatgt accttcatgc aatcacttct gtgcatgtgt
    52201 tggataaaga caatccacac gtgccacttt ttacagaaaa ctagattagt aggatgagct
    52261 gtgtaaaata gaaggttaac aaaaaagcga gtaaacgaat gtcctgtcat ttaggttttc
    52321 gtttactcgc tttttgatga gattttactg ctgtcctagc aaaaggtgag gatgaggtcg
    52381 tatatcacat agaggttttc tgttaagcct tatttgttac taaagaagaa aggtggcgtg
    52441 taatgactaa gggtttcgta acaagccttg gcttgctcat tgtcttcaca gacaatcaag
    52501 caagtatcat caccacagac cgttgctaca atttctggaa tttgcatggc atctaaaatg
    52561 gagccaaagg attgggctag tccaggtaac gtttttaaaa taatttgatg ttgaactatc
    52621 tttaacatga ccaaagcgtc ttccatgtaa aaacgaagcc gatgttccca gcgggtttgt
    52681 gaaatggcta acgcttcata gtgtgtatcg tttccagaag ttactttgac caggttcaat
    52741 tccttcatgt cacgtgatag ggtagcctga gtaatcgtga tgccattttt ttgcaaccgt
    52801 tcttgtaatt cttgttgtgt atgtatagtt gtctcagaga tgagggaacg tatgagttgg
    52861 tgacgcgtct ctttcttatt cattgttata atcttccttt aacgtaattg ataacaatcc
    52921 gagtatagca aaaagaggag aaggtgtaaa gtttttgatg aaccgcttac aaaatggctt
    52981 ttttatggta aaataaagat gatgtttaga aaaaggagat ttgtgtgatt cgaagagaag
    53041 attatcaata ccttagaaaa ctaaacgatt ttcgctactt ttctattgaa caatttgaca
    53101 aaattgtagg gcagatggaa ttccgaaaag ctaaaaagga ccatatcctt ttttttgagg
    53161 gagataaaag agataagctt tttctagtga cctctggtta tttcaaggtg gagcagtccg
    53221 atcaatcagg aaccttcacg tatacggatt tcatcagaca cggcaccatt tttccttacg
    53281 gtggcctctt tacagatgat tattaccatt tttcagtggt agccatgaca gacgtgactt
    53341 atttttactt tccagtcgat ttatttgaag attattcgct tgaaaatcgt ctacaaatga
    53401 aacacttata tagtaagatg tctaagctgc tcgagttaca tgagttgagg gtccgaaact
    53461 tgattacatc aagtgctagt tctcgtgtga ttcaatctct ggcgattttg ctggttgaaa
    53521 tgggtaaaga cagtgatacc ttaccttttc aattaacaac aacagatatt gctcaaataa
    53581 gtgggacgac aagagaaaca gttagccatg tgcttagaga tttgaagaag caagaattga
    53641 ttaccataaa aggtaagtat ttgacttatc tagataaaga ctatttttta caatacacaa
    53701 agtgaattaa aggtcagaac actttatttt aaaagttttt ctgacttatt ttaatttata
    53761 agtataaatt tttacactaa gatgtaaaaa acatacaagt aaacgaatac aaatctataa
    53821 aaataaaaac aatgctcaaa aaatgtaagc gattactcaa aatagctgtg agaaataccc
    53881 tatttttatc ttttttaagg agtacactag gaatataagc aagagttaag aatcttgacg
    53941 gtgaattcac aaaaaccaat tagaaagagg taattcttat gactgctcaa acaccaattc
    54001 atgtttattc tgaaattgga aaattaaaaa aagtgttgct ccacagaccc ggtaaagaaa
    54061 ttgaaaatct tatgccagac tacttggaac gcttgttgtt tgatgacatt ccattcttag
    54121 aggatgccca aaaagaacat gatgcttttg cccaagcttt acgtgacgaa ggtattgaag
    54181 tcttgtacct tgagacctta gctgcagaat ctcttgtcac tccagaaatt cgcgaagctt
    54241 tcattgatga atacctaagt gaagctaata tccgtggaag agctactaaa aaagccattc
    54301 gtgagttgtt gatggctatc gaagacaatc aagaattgat tgaaaaaacc atggcagggg
    54361 ttcaaaaatc tgaacttcca gagatcccag catctgaaaa aggcttgact gacttggttg
    54421 aatccagtta cccatttgcg atcgacccaa tgccaaacct ttatttcaca cgggacccat
    54481 tcgcaactat cggtacaggt gtttctctta accacatgtt ctcagaaacc cgtaaccgtg
    54541 aaacccttta tggtaaatac attttcactc atcacccaat ttatggtggt ggcaaagtgc
    54601 ctatggttta tgaccgtaat gaaaccactc gcattgaagg tggggacgaa ctcgttcttt
    54661 caaaagatgt gcttgcggtt ggtatttctc aacgtacaga tgctgcttct attgaaaaat
    54721 tgttggttaa catctttaaa caaaaccttg gcttcaagaa agtattggcc tttgaatttg
    54781 caaataaccg taaattcatg cacttagaca ctgtctttac catggttgac tatgacaaat
    54841 tcaccattca cccagaaatt gaaggcgatc ttcgcgttta ctctgtgact tatgacaacg
    54901 aagaacttca tattgttgaa gaaaaaggtg atttagcaga acttcttgct gctaacctag
    54961 gtgttgaaaa agttgacctt atccgttgtg gtggtgacaa cttagtagca gcaggtcgtg
    55021 aacaatggaa cgatggttct aataccttga ccatcgcacc aggtgtggtt gtggtttata
    55081 accgtaacac cattaccaat gccattcttg aatctaaagg cttgaaattg atcaagattc
    55141 acggaagtga attggttcgc ggtcgtggtg gacctcgttg tatgtcaatg ccatttgaac
    55201 gtgaagatat ttaataagct atggtaaagg tggttatagg ccagaagcct ttttaaaggg
    55261 cagctagtgt ttatcttcgc ttctgtgtct ttgtccttaa atgaaatttt gtcatggcag
    55321 ataaatttga tgccaatgac gaaacaagaa cggtttatgc agtcgtttac gacaatgacc
    55381 agcccgtttc aacaggacaa tttttagctg aaacgaaaat agaagcacga ttgacacgca
    55441 ttgtgacctt agcagattat tgtggttgcg gttatggtgc caaagtcact gaagcgctcg
    55501 aaacttatac cagacgagaa ggcttttacc aactaaccat tcacagtgaa ctgacagcac
    55561 aaacctttta tgaaaaccta ggttatcagt cctatggtcc caagtgttta gaagatggtg
    55621 agtattgtca gtcccttgca aaaaccattc ttaaatggga gaagaatatg gacatagcaa
    55681 tgctaattgc gattgttggt ggtctattag gctgctatct ctatctcaca aaaaataatt
    55741 aatcaaaaca gattacagtt aacactcgaa ggagacaata gatgacacaa gtatttcaag
    55801 gacgtagctt cctagcagaa aaagatttta cacgcgctga attagaatac cttattgatt
    55861 tttcagcaca tttgaaagat ttgaaaaaac gtggtgtgcc tcatcactac ttagaaggta
    55921 aaaacattgc cctcttgttt gaaaaaacat caactcgtac gcgtgcagct tttacaacag
    55981 cagccattga cctaggtgct cacccagaat acctcggtgc caatgacatc caacttggta
    56041 aaaaagaatc aacagaagac actgctaaag tattgggtcg tatgtttgat gggattgaat
    56101 tccgtggctt tagccaacgc atggttgaag aattggcaga attctcaggt gtgccagttt
    56161 ggaatggttt gactgatgaa tggcacccaa cccaaatgct tgctgactat ttaacagtga
    56221 aagaaaactt tggcaaactt gaaggcctta cccttgttta ctgtggtgac gggcgtaata
    56281 acgttgccaa ttcccttctt gtgactggtg ctatccttgg tgttaatgtt catatcttct
    56341 caccaaaaga acttttccca gaagaagaga ttgtgacatt ggctgaaggc tatgctaaag
    56401 aaagtggcgc tcgtatcctt atcacagaag atgctgacga agctgttaaa ggcgcagacg
    56461 tgctttacac agatgtttgg gtatcaatgg gtgaagaaga caaattcaaa gaacgtgttg
    56521 aattgcttca accatatcaa gtcaacatgg accttgttca aaaagcagga aatgacaagc
    56581 ttatcttctt gcactgcttg ccagctttcc atgacacaaa taccgtttat ggtaaagacg
    56641 ttgctgaaaa attcggcgtg aaagaaatgg aagtcactga tgaagtcttc cgcagcaaat
    56701 acgctcgtca ttttgaccaa gctgaaaacc gcatgcacac tatcaaagcg gttatggcag
    56761 caacccttgg taacctcttt attccaaaag tgtaataagg actggtactc cttaactcat
    56821 ttcacatatc aatgtgaccc ctttaattta acaatttaac tacttaaaaa cgtactttaa
    56881 ctacatgaca agtcgttctg gttttcgcgt ccttgaccgg aactagaacg gcttattttt
    56941 atgaaaaggt aagaggtaat cattatgaca gaagaaaaaa aacggggttt tagaattcct
    57001 tcttcttaca ccgttctttt tatcattata gccatcatgg cagtgttaac ttggtttatt
    57061 ccagctggcg cctacgaaac cgctaagggc ggtggtgtta tttcaggaac ctacaaaaca
    57121 gtagcatcca atcctcaagg attttttgac atcttgatgg ctcccgttcg aggcatgtta
    57181 ggtgttgaag gcactgatgg tgctattcaa gtgtctttct ttatcttaat ggttgggggc
    57241 tttttaggag ttgtcaacaa aacaggtgct cttgacacag ggattgcgtc agtggttcgt
    57301 aaaaacaaag gtagagaaaa aatgctgatt gctattttaa tccctttgtt tgctctaggt
    57361 ggaacgacct atggtatggg ggaagaaacc atggcctttt acccactttt aattcctgtt
    57421 atgattgcgg ttggttttga cagtattgtt gctgtagcca ttattttgat tggttctcaa
    57481 attgggtgtc tagcctccac tattaaccca tttgcaactg gtgtagcagc tgatgctgct
    57541 ggtgtcagca ttgcagatgg aatgatttgg cgtgttatcc aatgggttat tcttgtcggc
    57601 atgtctattt ggtttgttta caattacgct agtaagattg aagaagaccc aagtaaatca
    57661 ttggttgcag acaaggaaga agagcataag gaactcttcc agttgcaaaa ttctggagaa
    57721 gacttaaaca agcgccaacg aaacgttttg accattttta ctttgacctt tgtcattatg
    57781 attcttagtt tgattccgtg ggaagatttt ggcatcaaat tctttactaa tatcaatact
    57841 tggttaacca caatgcctat cttaggggga gttattggga aaaccatggg agcatttggt
    57901 acttggtatt tcccagaaat caccatgctc tttatcatga tgggtgtctt agttgctatt
    57961 gtttatcgta tgagtgaaga agactttttt agctcttttt tgactggtgc aggtgaattt
    58021 ttaggtgttg ccatgatctg tgctattgca cgcggtattc aagttatcat gaatggtggt
    58081 atgattacag ccactatctt acacttaggt gaaacaagtc tttctggttt atcttctcaa
    58141 gtttttgtga tattagctta tattttctac ctcccaatgt ccttcttgat tccatcaaca
    58201 tcaggacttg ctggggctac aatgggaatt atggcaccgc ttggacaatt ctcaaatgtc
    58261 cctgctcacc ttgttattac agccttccag tcagcttctg gaatcttaaa catgatttct
    58321 ccaacttcag caatcgttat gggagcactt gcgcttggtc gcgttgacct tggtacttgg
    58381 tggaaattca ttggtaaatt tattgtaatg gtgatgcttg taagcgtgct attacttgta
    58441 gttgcaacat tcttttaaca gataggaggt tcctatggaa tcctatatca cacctaaaca
    58501 tcaagaggct tgtgtagctg ccattaaaca gatcgtttct tacccttctg tttgtcatga
    58561 aggagaaaat ggcacacctt ttggacaggc cattcaagat gttctcgaag caaccttaga
    58621 cttatgtcaa ggtcttggct tccatactta tattgatcct aaaggttact atggttatgc
    58681 tgagttaggt gaccaaaaag aggtcttggc tattctgtgc catttggacg ttgttccaga
    58741 aggagatcgt cagttatgga agacagagcc ttttgactgt gttgaggcag acggctgttt
    58801 atttggtcga ggcactcaag acgataaagg gcctactatg atggcactat ttgccaccaa
    58861 agccttgata gatgcaggcg ttacctttaa taaacgcatt cgttttattt tcggaacaga
    58921 tgaagaaact ttgtggcggt gtatgaatcg ctacaatgat gtagaagaac aggctacctt
    58981 tgggtttgct cctgattcaa gtttcccttt aacctatgcc gaaaaaggcc tacttcaggc
    59041 aaaattagtg gggaaaggtt ctgataaact gtcacttgaa atcggacaag cctacaatgt
    59101 tgttcctgca agagcctctt atcaaggtga taagcttgag gctttacaaa aagaactaga
    59161 taagcttggc tttgagtacg tcgtcaaaga gggagaatta acggtttatg gtctcgccca
    59221 acatgctaaa gatgctcccg atggcatcaa cgctatgatt aggcttgcca aggctttagt
    59281 ggtccttgaa cctaacaaaa cccttgattt tcttgctaat gtcattgatg aagatggcag
    59341 agcacttaat atttttggca gaatcgaaga tgaaccttca ggcaaattaa gctttaatgc
    59401 cgcagggctt accttaacca aagacaaggc agaaatttgc ctagatattc ggattccagt
    59461 tttggcagat aaggaaaaat tagtccaaca attaagccaa aaagcacaag aatacggcct
    59521 aacctatgaa gaatttgatt atttggcccc attatatgtg ccattagaca gtgaattggt
    59581 aacaaccttg ttatctgtct atcggaaaaa aacaggtgat caaagccctg cccaatcatc
    59641 aggaggggca acctttgcta gaacaatgaa gaattgtgtt gcctttggtg cccttttccc
    59701 tgatgctgct caaacggaac accaagaaaa tgaacatatt gttttagcgg atgcttaccg
    59761 cgctatggat atttatgcgg aagctattta ccgactaacg cgataacaag atatgaggag
    59821 taattatgac gaaacaaaaa atcgtagtcg cacttggagg aaatgctatt ctttcaacag
    59881 atgcgtcagc aaaagcacaa caagaagcct tgatttccac atcaaaatca ttggttaaat
    59941 taattaaaga aggtcatgaa gtcatcgtga cccacggcaa tggcccacaa gttggtaatc
    60001 tcttattaca acaagcagcg gcagattctg aaaagaatcc ggctatgcct ttggatactt
    60061 gtgttgccat gacagaaggt agcatcggtt tttggttagt taatgccctt gataatgaat
    60121 tgcaagccca aggtattcaa aaagaagtgg cagctgttgt cactcaagtg atcgtggatg
    60181 ccaaagaccc agcttttgaa aacccaacaa aaccaattgg tccatttctt accgaagaag
    60241 atgccaaaaa acaaatggcg gagtctggtg caagtttcaa agaagatgca ggtcgtggct
    60301 ggcgtaaggt tgtgccatca ccaaaaccag ttggtattaa agaagccaat gtcatcagaa
    60361 gtctcgttga ttctggcgtt gttgtggtta gtgctggtgg tggcggtgtg cccgttgtgg
    60421 aagatgctac tagcaagacc ctgactggtg tggaagctgt tattgacaag gactttgcta
    60481 gtcaaaccct ttcagaattg gtggacgcag acctctttat cgttttgaca ggagttgata
    60541 atgtttacgt caacttcaac aaacctgacc aagccaaatt ggaagaagtg actgtctctc
    60601 aaatgaaaga gtacatcaca caagaccaat ttgcaccagg tagtatgtta ccaaaagtgg
    60661 aagctgccat tgcttttgtt gaaaacaaac caaatgctaa agccattatc acatcgctcg
    60721 aaaatattga caatgtacta tcagcaaacg ctggaacaca aattatcgca gggtaatcaa
    60781 gaggattttt agttctgttt aaaagactca tttaatggta ttttctgaag agtacgactg
    60841 acataaagag ttgatactgt ttttaaagga aaagagtgtg ccataataaa aaacaataat
    60901 agtattagaa aaggctaatt ttattgagaa ttagcctttt cttttagctt tgataggtgt
    60961 ataataacca tactattttt tatttttcaa aatattagaa taaacaaaag tggacaacgt
    61021 ataaagagaa ctgctaagtc gttgagttca ttagtcccaa aaagattacc aaaaatgaag
    61081 gaggagctat gaagaaaagt tttattcatc aacaagagga gatttccttt gttaagaata
    61141 cgttcactca gtatttgatt gctaagttag atgttgttga agtgcaaggc cccattttaa
    61201 gtcgggtcgg ggacggtatg caagataatt tgtccggtac tgaaaatcca gtatctgtca
    61261 atgtcttgaa aattcctaat gcaacctttg aagttgtgca ttcattagca aaatggaaac
    61321 gtcacacctt agcccgtttt gggtttaatg aaggtgaagg ccttgttgtt aatatgaagg
    61381 ctctccgacc tgatgaggat tcacttgacc aaacccattc tgtctatgtt gaccaatggg
    61441 actgggaaaa agtcatccct gatggcaaac gtaatctcgc ttatctcaaa gaaaccgttg
    61501 aaaccattta caaggtcatt cggttaactg aattagctgt agaagctcgt tatgatattg
    61561 aggctgtttt acctaagaaa ataaccttca ttcataccga agaattggta gctaaatacc
    61621 ctgatttgac gcccaaagaa cgtgaaaatg ccattactaa agaatttgga gcagtctttt
    61681 taattggtat tggcggggtt ttacctgacg gtaaaccaca cgatggccgc gctcctgact
    61741 atgatgactg gacaacagaa actgaaaatg gctatcatgg gcttaatggt gatattttgg
    61801 tttggaatga ccaacttggt tcagcctttg aattatcctc aatggggatc cgtgtggatg
    61861 aagaggccct aaaacgtcag gtggaaatga ctggtgacca agaccgctta ggctttgatt
    61921 ggcacaaatc tttgttgaat ggacttttcc ctctaacgat tggtggagga atcggtcaat
    61981 cacgtatggt catgtttttg ttgcgtaaac aacatattgg agaagttcag acctccgttt
    62041 ggccccaaga agttcgtgat agctacgata atattttata agtaatcaca tcattttgct
    62101 aagttttcca cttgtataag agactttaaa taaatgatca gaaatattga aaaaagaaat
    62161 catttttaag gtttagagct agaaatttgc tataataaat gttatgagag ttgtatcagg
    62221 tgaatttggt gggcgtcctt taaagacgct cgatggaaag ataacacgcc ctacttcaga
    62281 caaagttaga ggtgcgattt ttaatatgat aggaccttac tttaatggtg gtcgtgtttt
    62341 agatttgttt gcaggttcag gtggattagc cattgaggct gtttctcgtg ggatgtctgc
    62401 tgcagttttg gttgagaaga atcgtaaagc gcaagctatt attcaagaca atattataat
    62461 gactaaagct gagaatcggt ttaccttgtt aaaaatggaa gctgagcgag ctattgattg
    62521 cttgactggt cgatttgact tagtcttttt agaccctcct tatgccaaag aaacaattgt
    62581 ggcaactatt gaagctttag cagctaaaaa cttattgagt gaacaggtta tggttgtctg
    62641 tgagacagat aaaacagtct tgttgccaaa agagattgca acgttaggta tttggaaaga
    62701 aaaaatttat ggaattagta aggtaacagt ttatgttaac taaaatagga ctttatacag
    62761 ggtcatttga tcctgttaca aacggtcatc tcgatattgt taaacgggca agtggcctat
    62821 ttgatcaaat atatgttggg atttttgata atcctaccaa aaaaagctat tttaaactag
    62881 aagttcgtaa agcaatgctt actcaagctc tggctgattt tacaaatgta attgtagtaa
    62941 cttcgcacga acgtttagct attgatgtag ctaaagaatt acgagtaact cacttgatta
    63001 gaggcttgcg aaatgcaact gactttgagt atgaagaaaa tttggaatat tttaatcacc
    63061 ttttagctcc aaatattgaa accgtctatt tgatatcaag aaataaatgg caggccctta
    63121 gctctagtcg tgtgagagag ctaattcatt ttcagtcttc cttagaaggt ttggttcctc
    63181 agtcggttat tgctcaagtg gagaaaatga atgaaaagac ttaaaaaaat caaatggtgg
    63241 ttagtgggtc tgctagcttt aatctctttg ttgctagcgt tattttttcc gctaccttat
    63301 tatattgaaa tgcctggagg cgcttacgat attcggactg tcttacaagt caatggcaaa
    63361 gaagacaaac gaaaaggagc ttaccagttt gttgcagtgg gcattagtcg tgccagcctc
    63421 gctcagctat tatatgcttg gctgacaccg tttactgaaa ttagtacagc agaagataca
    63481 acaggcggat acagcgatgc tgatttcctt cgaattaatc aattttacat ggaaacatca
    63541 caaaatgcag ctatttatca agctttatcc ttagctggaa aaccagttac attagattat
    63601 aaaggcgtat atgttttaga cgtaaacaac gaatctactt ttaaaggaac gctacactta
    63661 gcagatactg taacaggtgt aaatggtaaa cagtttacta gttcagcaga acttattgac
    63721 tatgtttctc acctaaaact aggggatgaa gttacggttc agtttacgag tgataataag
    63781 cctaaaaaag gagttggccg tattatcaaa ctgaaaaatg ggaaaaatgg gattggcatt
    63841 gccttgactg atcatacaag tgtcaattca gaagacacag tgatctttag tactaaagga
    63901 gtaggaggac ctagtgctgg tctaatgttt actcttgata tatatgatca aataactaaa
    63961 gaagatttac gcaagggccg tacaattgca ggtacaggaa ctattggcaa ggatggcgaa
    64021 gtaggagata ttggtggtgc aggtcttaaa gtagttgcag cagctgaagc tggtgcagat
    64081 atattttttg ttccgaataa tcctgttgat aaggaaatta aaaaagttaa tccaaatgct
    64141 ataagtaatt acgaagaagc caaacgggca gccaaacgac taaagaccaa aatgaagatt
    64201 gttcctgtta cgactgttca agaggcactg gtttatcttc gcaaataaaa taactatgaa
    64261 tgtattatat tcaattatat tcacgctatt aactcataaa aaacggttga accagataga
    64321 cagatccttg accagtcttt tgggttcaat cattttttac ataaaacata tctaacttga
    64381 cagcgtaaaa aagatgttat aaaagtctag tggtaaaaag gcaatagaga attgagtaaa
    64441 ctttagtcga ttatctggaa atagagcata ctaaaaacac ctagtgaaaa ttgatttttt
    64501 tcactagctc atccaagaac ttgcgcagat aatcatgagt ccattccatg actctattat
    64561 tctggaaagc cttgcaatag acaagttaac gttataacaa ctaaattaaa aacttgagat
    64621 tgaaaaatcc ccataaactt attttacaag gaggtaaggc tatggtaacc attaaacaag
    64681 ttgctgaaga agcaggtgtt tctagatcaa cagtctccag gtatatttct caaaaaggat
    64741 atgtatctga tgatgcaagg cataaaatca aagcagcgat agcaaaatta cattacactc
    64801 caaatgtctt ggcgcagtca ttgaagacga aaaaaaatca actagtcggt ttattattac
    64861 cagacatttc caatcctttt tttccgaggt tagcaagagg agcagaagaa tatttaaaag
    64921 aaaaggggta tcgggttatg ctaggaaata tttcagacag cgaagctctt gaagaagaat
    64981 acgtccatgt ccttcttcaa agcaatgctg ctggcattat tactacccat gactttacaa
    65041 agcggtatcc tactcttgct attccagttg tagtggtaga tcgtgttgac caagagaccc
    65101 agtacggtgt cttttcagat aatagggcag ggggcttatt ggcggctcag actgtctggc
    65161 aagcaggggc aaaagaagtt ttgttaataa gaggtccgct ggataatgct gaaaacatta
    65221 atgagcggtt tgaagctagt ttttcttacc ttcaaaaaca agacgtgact atgtatgtgt
    65281 gtgatagtca gaattttgat tttgaaagta ttcagctaga agctagttac aacctcaagt
    65341 gttacccaac aatagacagt attatagcac cttctgatat ccatgctatt gcttatattc
    65401 atgaactgca ttctcaaggt aaaaagattc ctcaagatgt tcaaataata ggttatgatg
    65461 acattttaat gagtcaattt atttatcctt ctttatcgac tattcatcaa tcctcttatt
    65521 tgatgggccg ttatgccgcc gaattagttt atactattgc tagtcaatta actgtgaaag
    65581 caaatcggat taaattgcct gttcactacg ttgagcgaga aacaataagg aaaagataag
    65641 taataatatt cgtccagatt ttagcaggta tgatgaagat ttattgggat ttaccttcta
    65701 ctttaaaaga agttatctgt cattactttc atcgctatca gtcaaaacgc attcttatgc
    65761 aactcttaac cttaagctta atgtcttttt gataacatag aggaatgtta cacgatgatg
    65821 acgcaataaa gcctaaatat agtaattgtc gcgattttat agttagtgag taatgagata
    65881 gcaagtctct catttactta caaaagtcaa aaaatagtgt ataatggttg agttacagat
    65941 aacactgcaa aaaagataac aagtgtccat aattatctaa gactataaag gcgatataga
    66001 cagatataaa aaagatgacg aatcagttat cactggacaa ttaaccgatt tagttacaac
    66061 taatggattt ttcagaaagg tgtcaaatga aaaaagaaat ttcgccagaa atgtacaact
    66121 ataataaatt tccaggtccc aaatttattc attttgaaga gcaagttaag gctgaaggca
    66181 ttgatttgtt actcttagaa gatgttaaga acgcttttga tacgactagc tttggtcaac
    66241 gttatacaga agttctccta aagtatgact atattgttgg cgattgggga aatgaacagc
    66301 ttcgtcttaa agggttttat aaggatagtg atgatatcaa gaaaacgaat cgcatctcac
    66361 gtttagaaga ttatattaaa gaattttgca attttggttg tgcttatttt gttttagaaa
    66421 atcttcatcc acaagatatt aaatttgaag aggagcgcca accaagacga aagaaatcac
    66481 ctaaatcaaa atcaaatcgt cgcaagccaa actattcaaa tcagcagccc gcaacaccta
    66541 agagtaaatc gaagcgggta tcaaaagaaa agcaacctga aaaccaagcg tttactagtc
    66601 aaaaacgtcg aagtaatact aagcataaag aaaagtcaaa acgtaatcag actagtcaac
    66661 ttaataccaa aattagtcct tttatcatca gaaagaaaga taaataatcc atgaaaccat
    66721 cgatttatag cttaacacgc gatgaactga tcgcgtgggc agttgaacgc ggccaaaaac
    66781 agtttagagc tactcaaatt tgggattggc tctataaaaa aagagttcaa tcttttgagg
    66841 agatgacgaa tatttctaaa gattttgttt ctattttaaa tgatagtttc tgtgtcaatc
    66901 ctttaaaaca gcgagttgta caagagtcag ctgatggtac agtaaaatat ttatttgaac
    66961 tgcccgatgg tatgttaatt gaaacagttt taatgcgtca acattatggt cattctgttt
    67021 gtgtgacgac tcaagttggc tgtaacattg gctgtacttt ttgtgctagt ggattgatca
    67081 aaaagcaacg tgatctaaac agtggggaaa ttacagctca aatcatgtta gtccaaaaat
    67141 attttgacga tagaaagcaa ggtgagcggg ttagccatgt tgtcgtaatg ggcattggtg
    67201 aaccttttga taattacaaa aatgtcatgt gctttcttcg cgtcatcaac gatgataatg
    67261 gcttagcaat tggtgcccgt catattacag tctctacctc agggttagct cacaagattc
    67321 gggattttgc aaacgaggga gtacaggtta atttagcagt ctcgttgcat gcaccaaata
    67381 atgatttacg ttcaagtatt atgcgagtaa atcgctcttt tccgctggaa aagctctttt
    67441 cagctataga atactatatt gaaaaaacga atcgtcgtgt cacttttgaa tacattatgc
    67501 taaatgaagt taacgatagc attaagcaag ctcaagaatt agcagactta actaaaacta
    67561 ttcgtaagtt atcttatgtt aacttgattc cgtataatcc tgtttcagaa catgatcagt
    67621 atagtagaag tcctaaagag cgagttctag cgttttatga tgtgctaaag aaaaatggag
    67681 ttaactgtgt ggtgcgtcaa gagcacggaa cagatattga tgcagcctgt ggccagttac
    67741 gttctaaaac gatgaaaaaa gatcgcgaaa aggttacagc gacaaaataa agcccaaaaa
    67801 atatctttga ttgacaatgc ttgatgacaa aatatttcta gtcactaaaa tgactaggtt
    67861 ttttatctgg cctaaaaatg ctctagaagc acaatccaaa ataatccaaa actaagataa
    67921 ggaataaaag gtatgcgctt gatgcctaat agtaagcaag cagtaatacc tgctaaacta
    67981 gcaagttgaa ttagccaaat caacgacgtt agatccaata ctaaagctaa gctagcaagg
    68041 tagaaaaaat cgcctgctcc aattgaaaaa ggtcttaaat gagctaataa acctagtagc
    68101 agtaatatca atgataaata attgatcgaa taaaacagga gtagacttac aaagctaaaa
    68161 agccacaaga taaaagggta ttcttgagaa cgccaatcaa atatactaaa taataaagaa
    68221 aagagtaaca agtaacagta actagcacta acccaatgta ccatactagc ccataagata
    68281 attattagac ttatctctaa gctaaagaag attttttggc tagttataaa atggttttgg
    68341 aactgccgaa aaacgcgttt ggcaaaagtt agttggtcac aaacaatgac agtctgttta
//
LOCUS       DAUCWU010000004        61738 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUCWU010000004 DAUCWU010000000
VERSION     DAUCWU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61738
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.004"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            142..744
                     /locus_tag="VGY24_000218"
     CDS             142..744
                     /locus_tag="VGY24_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283238.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            784..2112
                     /locus_tag="VGY24_000219"
     CDS             784..2112
                     /locus_tag="VGY24_000219"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ4283239.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMKLIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMILVSEHLKTFTGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            2158..2640
                     /locus_tag="VGY24_000220"
     CDS             2158..2640
                     /locus_tag="VGY24_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4283240.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            2752..4326
                     /gene="mltG"
                     /locus_tag="VGY24_000221"
     CDS             2752..4326
                     /gene="mltG"
                     /locus_tag="VGY24_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ4283241.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVAQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            4399..4881
                     /gene="greA"
                     /locus_tag="VGY24_000222"
     CDS             4399..4881
                     /gene="greA"
                     /locus_tag="VGY24_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ4283242.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(5506..6429)
                     /gene="yidC"
                     /locus_tag="VGY24_000223"
     CDS             complement(5506..6429)
                     /gene="yidC"
                     /locus_tag="VGY24_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ4283243.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQANSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMIAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(6511..6789)
                     /locus_tag="VGY24_000224"
     CDS             complement(6511..6789)
                     /locus_tag="VGY24_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ4283244.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDICGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            7050..7787
                     /locus_tag="VGY24_000225"
     CDS             7050..7787
                     /locus_tag="VGY24_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ4283245.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYETPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            7826..8326
                     /locus_tag="VGY24_000226"
     CDS             7826..8326
                     /locus_tag="VGY24_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ4283246.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            8341..9030
                     /locus_tag="VGY24_000227"
     CDS             8341..9030
                     /locus_tag="VGY24_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ4283247.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            9208..9450
                     /locus_tag="VGY24_000228"
     CDS             9208..9450
                     /locus_tag="VGY24_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ4283248.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            9628..10422
                     /gene="racE"
                     /locus_tag="VGY24_000229"
     CDS             9628..10422
                     /gene="racE"
                     /locus_tag="VGY24_000229"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ4283249.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            10419..11405
                     /locus_tag="VGY24_000230"
     CDS             10419..11405
                     /locus_tag="VGY24_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ4283250.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQREIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDTILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            11384..11905
                     /locus_tag="VGY24_000231"
     CDS             11384..11905
                     /locus_tag="VGY24_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ4283251.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            11902..12363
                     /locus_tag="VGY24_000232"
     CDS             11902..12363
                     /locus_tag="VGY24_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ4283252.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            12360..13106
                     /gene="xerD"
                     /locus_tag="VGY24_000233"
     CDS             12360..13106
                     /gene="xerD"
                     /locus_tag="VGY24_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ4283253.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKAMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPMTLEKYYKS"
     gene            13106..13810
                     /locus_tag="VGY24_000234"
     CDS             13106..13810
                     /locus_tag="VGY24_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ4283254.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKNNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            13807..14358
                     /locus_tag="VGY24_000235"
     CDS             13807..14358
                     /locus_tag="VGY24_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ4283255.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            14480..15202
                     /locus_tag="VGY24_000236"
     CDS             14480..15202
                     /locus_tag="VGY24_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4283256.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            15199..15462
                     /gene="yidD"
                     /locus_tag="VGY24_000237"
     CDS             15199..15462
                     /gene="yidD"
                     /locus_tag="VGY24_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ4283257.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            15640..16188
                     /locus_tag="VGY24_000238"
     CDS             15640..16188
                     /locus_tag="VGY24_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEQ4283258.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      16297..16421
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16500..17063
                     /locus_tag="VGY24_000239"
     CDS             16500..17063
                     /locus_tag="VGY24_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4283259.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            17065..17718
                     /locus_tag="VGY24_000240"
     CDS             17065..17718
                     /locus_tag="VGY24_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ4283260.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQN"
     gene            18011..18931
                     /locus_tag="VGY24_000241"
     CDS             18011..18931
                     /locus_tag="VGY24_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ4283261.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMINTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            18970..19524
                     /locus_tag="VGY24_000242"
     CDS             18970..19524
                     /locus_tag="VGY24_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4283262.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            19657..20991
                     /locus_tag="VGY24_000243"
     CDS             19657..20991
                     /locus_tag="VGY24_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ4283263.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            21069..21860
                     /gene="pflA"
                     /locus_tag="VGY24_000244"
     CDS             21069..21860
                     /gene="pflA"
                     /locus_tag="VGY24_000244"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ4283264.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            21991..22926
                     /locus_tag="VGY24_000245"
     CDS             21991..22926
                     /locus_tag="VGY24_000245"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ4283265.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDISEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            23002..23655
                     /locus_tag="VGY24_000246"
     CDS             23002..23655
                     /locus_tag="VGY24_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ4283266.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVNTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(23700..24737)
                     /locus_tag="VGY24_000247"
     CDS             complement(23700..24737)
                     /locus_tag="VGY24_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4283267.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLISLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(24698..25750)
                     /locus_tag="VGY24_000248"
     CDS             complement(24698..25750)
                     /locus_tag="VGY24_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4283268.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALILAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(25740..26672)
                     /locus_tag="VGY24_000249"
     CDS             complement(25740..26672)
                     /locus_tag="VGY24_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ4283269.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEIIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(26698..27480)
                     /locus_tag="VGY24_000250"
     CDS             complement(26698..27480)
                     /locus_tag="VGY24_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283270.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(27726..29171)
                     /locus_tag="VGY24_000251"
     CDS             complement(27726..29171)
                     /locus_tag="VGY24_000251"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ4283271.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQDQLKLLGFTGTKGKTTAAYFAYHILKESYKPALLSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVAINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            29259..30893
                     /locus_tag="VGY24_000252"
     CDS             29259..30893
                     /locus_tag="VGY24_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ4283272.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAVSQIAEQLIRVIWMLLTTFFIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLHQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYVFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            31061..31690
                     /gene="upp"
                     /locus_tag="VGY24_000253"
     CDS             31061..31690
                     /gene="upp"
                     /locus_tag="VGY24_000253"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ4283273.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            31914..32504
                     /locus_tag="VGY24_000254"
     CDS             31914..32504
                     /locus_tag="VGY24_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ4283274.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            32996..33271
                     /locus_tag="VGY24_000255"
     CDS             32996..33271
                     /locus_tag="VGY24_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ4283275.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            33520..34155
                     /locus_tag="VGY24_000256"
     CDS             33520..34155
                     /locus_tag="VGY24_000256"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ4283276.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     IAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLTKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            34173..35048
                     /locus_tag="VGY24_000257"
     CDS             34173..35048
                     /locus_tag="VGY24_000257"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ4283277.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEDMVLFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHTNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            35060..35306
                     /locus_tag="VGY24_000258"
                     /pseudo
     CDS             35060..35306
                     /locus_tag="VGY24_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
     gene            35711..36034
                     /gene="yabA"
                     /locus_tag="VGY24_000259"
     CDS             35711..36034
                     /gene="yabA"
                     /locus_tag="VGY24_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ4283278.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            36039..36902
                     /gene="rsmI"
                     /locus_tag="VGY24_000260"
     CDS             36039..36902
                     /gene="rsmI"
                     /locus_tag="VGY24_000260"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ4283279.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLANMLTCYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVSGSQVDSEVELTAEVDLVSLVQKEIQAGAKPNQAIKTVAKAYQVN
                     RQELYQQFHDL"
     gene            36929..37321
                     /locus_tag="VGY24_000261"
     CDS             36929..37321
                     /locus_tag="VGY24_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283280.1"
                     /translation="MTEDQTAVLILGTYGIILAIFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFIKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIATLIIAFGKAYTAEEYHPVPHLLN"
     gene            complement(37368..37997)
                     /locus_tag="VGY24_000262"
     CDS             complement(37368..37997)
                     /locus_tag="VGY24_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ4283281.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVTRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKNISVATMIRPRGGDFVYNDLELKAMEEDILKAIEAGSDALVLGLLTSENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQVLDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKDLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            38295..38651
                     /locus_tag="VGY24_000263"
     CDS             38295..38651
                     /locus_tag="VGY24_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ4283282.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGK
                     VLQVGYRKPYQELDLG"
     gene            complement(38725..39552)
                     /gene="xth"
                     /locus_tag="VGY24_000264"
     CDS             complement(38725..39552)
                     /gene="xth"
                     /locus_tag="VGY24_000264"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ4283283.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHVHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            39786..40967
                     /gene="lctO"
                     /locus_tag="VGY24_000265"
     CDS             39786..40967
                     /gene="lctO"
                     /locus_tag="VGY24_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ4283284.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSTGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            41232..46169
                     /gene="scpC"
                     /locus_tag="VGY24_000266"
     CDS             41232..46169
                     /gene="scpC"
                     /locus_tag="VGY24_000266"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ4283285.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLVMTTTVAADELSTMSEPT
                     ITNHAQQQAQHLTNTELSSAESKTQDTSQITPKTNREKEQPQGLVSEPTTTELADTDA
                     ASMADTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFGDFD
                     EDWENFEFDAEPKAIKKNKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDETKYES
                     HGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIEDAV
                     ALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLA
                     TNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVDFK
                     NIKDSLGYDKSHQFAYVKESTDAGYKAQDVKDKIALIERDPNKTYDEMIALAKKHGAL
                     GVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVSKA
                     PSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGA
                     SLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDG
                     AVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQKG
                     RFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRFRD
                     SQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGKHF
                     TGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNNQD
                     FAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLG
                     TAFSGKSLTGAELPDGHYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPET
                     NRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVADNKTFVERQADG
                     SFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKETL
                     KDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKGLK
                     NNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQYV
                     VTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVREEV
                     FYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRA
                     GNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNN
                     SGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQIT
                     AHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRIEG
                     NTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSATAK
                     ALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            46958..47665
                     /locus_tag="VGY24_000267"
     CDS             46958..47665
                     /locus_tag="VGY24_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ4283286.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSFIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            47905..49905
                     /gene="metG"
                     /locus_tag="VGY24_000268"
     CDS             47905..49905
                     /gene="metG"
                     /locus_tag="VGY24_000268"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ4283287.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDEDGKVIGGIAPSGH
                     QVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADFSQLIDAQLADYHKHMEAVDYPRALEAIWTVIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMVHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            50397..51410
                     /gene="nrdF"
                     /locus_tag="VGY24_000269"
     CDS             50397..51410
                     /gene="nrdF"
                     /locus_tag="VGY24_000269"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ4283288.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            51414..51902
                     /gene="nrdI"
                     /locus_tag="VGY24_000270"
     CDS             51414..51902
                     /gene="nrdI"
                     /locus_tag="VGY24_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ4283289.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFAIAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            51869..54049
                     /gene="nrdE"
                     /locus_tag="VGY24_000271"
     CDS             51869..54049
                     /gene="nrdE"
                     /locus_tag="VGY24_000271"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ4283290.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(54252..55004)
                     /locus_tag="VGY24_000272"
     CDS             complement(54252..55004)
                     /locus_tag="VGY24_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ4283291.1"
                     /translation="MLKKRYQLAMILLLSCFSLIWQTEGLVELFVCEYYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLVYPRTEQEAMAAYTCQQAALINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADITSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEACFKGSL"
     gene            55463..55897
                     /locus_tag="VGY24_000273"
     CDS             55463..55897
                     /locus_tag="VGY24_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283292.1"
                     /translation="MKTRSKRFLNLATLCLALLGITLLMARPVKAEFVPTHQQSGESS
                     RLMNDPGEDSSSEEMKRAKDRKCGYDEGYELGQESDHRKVDRKSIEVPYYVEDQDEYK
                     DGYEQGFEAGWYDSHPIAALLDMAWYYLTYTFDTLFGGAKSQ"
     gene            56386..56772
                     /locus_tag="VGY24_000274"
     CDS             56386..56772
                     /locus_tag="VGY24_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283293.1"
                     /translation="MKTKSKRFLKLATLCLALLSTTLLMAQPVKARDNQSQQQEHVSN
                     NIVEDSQKNDSDYDQGYEEGYKEGQKEGAPENPDKKDEDKLKNKPPRYGDGYYSGYYR
                     GWDVTNRPFQTLLKDIYLWLWETFFK"
     gene            57450..57929
                     /locus_tag="VGY24_000275"
     CDS             57450..57929
                     /locus_tag="VGY24_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283294.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLIAQPVKAEVTVATTEGVHQT
                     AEAENEDIIKEEYMTLLGLSDSDLQDPLEEARYKGYVDGYRKGFGGEDRIDRSAIKDL
                     YYNVPSSEDGEYRDGYEQGFEKGRHANYPVRAFLEDLLGGLWGFLTGIFGDGSSDTQ"
     gene            complement(58229..59483)
                     /locus_tag="VGY24_000276"
                     /pseudo
     CDS             complement(58229..59483)
                     /locus_tag="VGY24_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            59409..59744
                     /locus_tag="VGY24_000277"
     CDS             59409..59744
                     /locus_tag="VGY24_000277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283295.1"
                     /translation="MKNLVQGDVFIFNSNQFCSMIELSHMILSNDGLVALHYKSYGIF
                     CVHPKSLIISVGELPTTDIIEPFFATYSYLYLPFVASSLAIVGKSKCSLAGLVVAVTV
                     QSEPTAIPY"
     gene            60000..60554
                     /locus_tag="VGY24_000278"
     CDS             60000..60554
                     /locus_tag="VGY24_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283296.1"
                     /translation="MKTKSKRFLNLVTLCLALLGTTLLMGRPVKAEEDSDIKKEYMKR
                     LMLTDSDLEPGGYFSKRVQGYLKGYADGQRKDAREPDSNSYGPNFDAYDGYKDGYDEG
                     YGEGWRKANEAGGSHSDESQSSGGTQDQDKVEKSTEGSHNEESQDNHEASKEGENSTD
                     DLTVSGLLSGIVETFVAFLWSLFN"
     gene            60858..61133
                     /locus_tag="VGY24_000279"
     CDS             60858..61133
                     /locus_tag="VGY24_000279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283297.1"
                     /translation="MNRESEQDDEAEAQDAGWQKGLEAGKNSSIWKPALEFDEVPQPD
                     VEYSDYVLQQYKEGYMAGYAYGWGETHELGLAVLSVLQWVWGSIFGY"
     gene            61299..61537
                     /locus_tag="VGY24_000280"
                     /pseudo
     CDS             61299..61537
                     /locus_tag="VGY24_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        19533 a        11480 c        12818 g        17907 t
ORIGIN      
        1 gatatagcga tttgtcattt gctagtaatg attaaaacat ttagaaaaag acaagtcata
       61 gaatcatcaa ggaatgcttt gaaaaataac agtatttaag ttataataga ggatgctaca
      121 agaagggaga atcaaaaaag gatggtaaaa aatcagtttc ctttagttgc agatggcatt
      181 gctattagtg atccagcaaa gcaaatggct ctgtatgaaa atgaagatct cattaccaac
      241 atccgtggct attaccagga caaggagtat gatgatattg ctagaaatga ggagtttact
      301 gctaaagcaa cttctcggca ggccccttct agtaagcgct tttgtagcaa tgatgaaaaa
      361 caccattatg tcaaagaggc gcgtcaaaaa gcaaagcaag atctcaagga aaaaagacag
      421 gcttatcttg ccaaagaaat ggcctatgtt cctaaacagg tttctaaaaa gcaacagcct
      481 gcggattctt ctccttctca aaagcaagca acaacagaaa tgagtcgttt tacaaaaaag
      541 cttcatcaag acaactatat tttagctgag ttgccaaaag aatataaaga gccaaaaaat
      601 cttcctcaac aaggaactac aaaaaaaaat aattacgatt ttttaaaatc cagtcaaatt
      661 tacaataata aagaaatgag acaacaacga gaaaaaacaa ttgcgcaaga gcttaatctt
      721 agtaggtttg aggatcttcc ttaataaatc tactcagcgt atgcagatga caaaggagtt
      781 aagatgtcaa aaacgtatca ttttatcgga attaaagggt caggaatgag tgccttggcc
      841 cttatgttgc atcaaatggg gcataaggtg caaggtagtg atgttgaaaa atactatttc
      901 actcaaagag gattggaaca agcgggcatc actatcttac cttttagcga ggacaacatt
      961 acgccagata tgaaacttat cgttgggaat gcctttcgtg aaaacaacaa agaagtggcc
     1021 tatgctttga gacaccagat tcctttcaaa cgttatcatg actttttagg tgattttatg
     1081 aaatcattta tcagttttgc agtagcaggt gctcatggta aaacttctac aacgggttta
     1141 ttgtcacatg tcttgaaaaa tatcacagac acatcttatt taattggtga tggtactggg
     1201 cgtgggtctg ctaatgcgca gtattttgtt tttgaatcag acgaatacga acgccatttt
     1261 atgccttatc acccagaata ctctatcatt actaatattg attttgacca tcctgattat
     1321 ttcacaggaa ttgctgatgt gcgaaacgct ttcaacgatt atgctaagca ggtcaagaaa
     1381 gctttgtttg tttatggtga agatgacgaa ctaaaaaaga tcgaagcacc tgcacctatt
     1441 tactattatg gttttgaaga gggcaatgat tttatcgctt acgacattac gcgtactacg
     1501 aacggatcgg actttaaggt aaaacaccaa ggagaggtca ttggtcagtt tcacgtacct
     1561 gcttatggaa aacataacat tttaaacgct acagcagtaa ttgcaaactt gtttgtagct
     1621 ggtattgaca tgatattagt ctcagagcac cttaaaacat ttactggtgt gaaacgtcgt
     1681 tttacagaga aaattatcaa tgacactatt attattgatg actttgctca tcatccaacc
     1741 gaaattgtgg caacaattga tgcggctaga caaaaatacc caagtaaaga aattgtagca
     1801 atattccagc cacatacttt tactcgcact attgccttat tagaggattt tgcttgtgct
     1861 ttaaatgaag ctgacagtgt ttatttagcc cagatttatg gttctgcacg tgaagtagat
     1921 aaaggtgaag ttaaggttga agatttggct gctaagatta tcaaaccatc acaagtagta
     1981 acggtggaaa atgtctctcc tcttcttgat cacgataatg ctgtttatgt ttttatggga
     2041 gctggagata ttcagctata tgaacattct tttgaggaat tacttgcaaa tttaactaaa
     2101 aataaccaat aacatgtcct gccttaggca ggttttctca ttctaaagga gtttgatatg
     2161 ttgattagac aggttcaagg atccgatttg gaagttattg ctaccattga aagtgacaat
     2221 ttttcaccac aagaagcaac gacaagagca gttcttgaag agcgcatacg gctgatacca
     2281 gatacttttt tagtggcact tatagatcag gaaattgttg gctatatcga aggtccagtt
     2341 gtcactactc ctattttgga ggattctttg ttccacgggg tgactaaaaa tcctaaaact
     2401 ggaggctata ttgctattac tagtttgtcc atagctaaac attttcagca acaaggggtt
     2461 gggacagctt tgttggcggc actaaaagat ttagtagtag cgcagcaaag aacaggtctt
     2521 attttgactt gtcatgatta cttgatttcc tattatgaaa tgaatgggtt tattaaccaa
     2581 ggaatttcag agtctcaaca tggtggaacc ctttggtacc aaatgatttg gaaactttag
     2641 aatcacagct gattcaactg ttggtttgtt aaaaataagt aacaaatgca tcatttaatt
     2701 gcaattagga tacttttaag ttataatggc ggttgattat atttaggagg attggctttg
     2761 accgatttta aggataaaga ccaacaagat cagcagcgaa gctttaaaga gcagattctt
     2821 gctgagttgg aaaaagcaaa tcaaatcaga aaagaaaaag aggaagaact ttttcaaaaa
     2881 gagttggaag ctaaagaagc agctaggaga acggcccagc tatatgctga atataaaaga
     2941 caagatgctt ttcaaaaaga gtctatagca cataacaata agacagctaa acactttcaa
     3001 gctataaaag gtgcggtaat gacttcagaa gcgcttaaac cgactttact ttctgaaaaa
     3061 gaaaactcat ctttaaaaac gacaaataag agagtcgcgc aggcaaatga gcttcaagag
     3121 actgcctcta aagaatctca agtaccgtta actattgaga aaggtcattc agtgagacga
     3181 aaattaagca aacgccaaca gactgagcga gctgctaaaa agatttcaac cgttttgatt
     3241 agttctatta ttataaccct tttggctgtt actctagcag gagcaggcta tgtttatagt
     3301 gctttaaatc ctgttgataa aaatagtgat gcctttgttc aagttgagat tccatctggg
     3361 tcaggcaata aattgattgg tcaaattctt caaaaaaaag gtttaatcaa gaatagcact
     3421 gtttttagtt tttatacaaa atttaaaaac tttacaaatt ttcagagcgg gtattacaat
     3481 ctgcaaaaaa gtatgagtct agaagaaatt gctagtgctt tacaagaagg tggtacagca
     3541 gaacctacca agccatctct tgggaagatc ttgattccag aaggatacac gattaaacaa
     3601 atagctaaag ctgttgagca taatagcaag ggaaagacca aaaaagctaa aacacctttt
     3661 aacgagaagg attttttgga tttagtcacg gatgaggctt ttattcaaga tatggtaaaa
     3721 agatatccaa aattattagc aactatccca actaaagaaa aagctattta ccgtttggaa
     3781 ggttaccttt tcccagcaac ctataactat tacaaagaaa ctaccatgag agaacttgta
     3841 gaggacatgc tggcagctat ggatgctact ttggtaccct attatgataa aattgctgct
     3901 agtggtaaga cagtcaacga ggtattgacc ttggcctctt tggttgaaaa agaaggttca
     3961 acagacgatg acagacgtca aattgcaagt gtcttttata accgccttaa tagcggaatg
     4021 gcactacaat ctaatatagc tattttgtat gcgatgggga aacttggtga gaaaacaacc
     4081 ttggctgagg atgctactat tgacaccacc attaattctc cttataatat ttataccaat
     4141 acaggtctga tgcctggtcc agttgctagc tcgggggttt ctgcaattga agcaacccta
     4201 aatccagcct caacggatta tttatacttt gtggccaatg tccatactgg tgaagtttac
     4261 tatgcaaaaa catttgaaga gcactctgca aatgttgaaa aatatgtgaa tagtcaaatt
     4321 cagtaaacac aaacatggtg cttaagcacc atgtttgtgt ttctgattcg ttcaaaaaag
     4381 aaaagagaga aaacaattat ggcagaaaaa acgtatccga tgaccctaac ggaaaaagaa
     4441 caacttgaaa aagaacttga agaattaaaa cttgtgcgtc gtccagaaat agtggagcgt
     4501 attaaaattg cacgttcgta tggagatctt tcagagaact ctgaatacga tgccgcaaaa
     4561 gatgagcaag cttttgttga aggtcaaatt tcaacgcttg aaactaagat tcgctatgct
     4621 gaaatcattg acagtgatgc tgttgcaaaa gatgaagtcg ctatcggaaa aacagttatc
     4681 gttcaagaag ttggaacaac agataaagat acctatcata ttgtaggtgc tgctggagca
     4741 gatatcttct ccggaaaaat ttcaaatgaa agcccgattg ctcaagcttt gattggtaaa
     4801 aaaacaggtg ataaggttag aattgaatca ccagcagcta cttatgatgt tgaaatcatc
     4861 agcgttgaaa agacaaatta atcagatgaa gccattattt gcctaaccac taagtgtttt
     4921 ggttgctaat ggcttttcta gtatcttcaa atgaggttga ctgttaggaa ggtcaagtat
     4981 taatcgcatt gattatgagc caagaggact atagatcaaa gatcgttttc gatggttatt
     5041 taataccaaa agaaaagacc agcaccttaa tgtactgacc ccaaaaagtt ggacactata
     5101 ttttaagtat aggaagtgta taatctaagg aaactgacac taatcattga ccgttttgca
     5161 agactttatt aatgatttct tgctttattt taaatgaata agtctgatac gtttccttcc
     5221 ttaacactat cgactccata atgacccatc agtcaaacgg tatatctcaa acgactacgg
     5281 tttactccaa attgattggc tagttgaggc caagaaacac ctgatttttt ttaatgctaa
     5341 atttctagct tatcttcata gcttaatttc atacaaaaac accccaatcg ttagattttt
     5401 ttgatatgtc ccctttcact agatgagtcc agtaaaaggg ggcatatcgt tttttgtcta
     5461 acttttggag tgcagttcat aattgtgcta ggttttttgt ctttcttaac gttttcgctg
     5521 ttttccagca ttacgattgg attttatttt tttaggcaag ttagcctgtt ccatgttttg
     5581 tgaaggcgta acgtccttac gagaagaagt tgattggtat gctttaggcg ggtttttagc
     5641 gtattcttct ttaatttgct tatgcaatcg aggttttaag aggtaggttg tgattaactg
     5701 ttgaataata ctaaagaaac caccaactag ccagtaaaga ccaacgccag cagggagtga
     5761 gaaggacatg aagatcatca tgataggcat ggtgtacatc atcgtcttca tttgctcacg
     5821 ttgttcttct gaaacggcta tcattgataa ccatgattgg aagaagtaaa gagcagcgat
     5881 aattgctgtt aacactaaac tacggctacc aagatcaatg cccataaagg tacttgttga
     5941 tactccttta gtgtattgag cggcaaaata catggcagaa aagaatggca tctggatgag
     6001 aagaggtaga catccaatac ctccaagcgg gttaatccca tgagcacgct gagcagccat
     6061 taattctgtt tgggcagcca ttttttcttc ctgactattt gcctgtttga tacgtttgtt
     6121 gattggttca aaaacaggtt tcaagaaggc cattttctca gattgataac ttgctttcca
     6181 agattgatag agacctagtg gtaagataag tgttctaaca ataatggtta cgatgatgat
     6241 agccagtcca taacctagtc cagcattgtt tgcaaagtaa tcgataaagt atgacatggg
     6301 ttttccaaga aattcccaaa tcataccttt gggatttccg tgagcgtctc ttcccacaca
     6361 gcctgtcaag gtgaatagaa ttgacagtgc tagtcctgaa aagaggatgc gatttaatgt
     6421 tagtttcaat gtgtttatcc tttaatttta atatacttat ctattttaca gtttttttca
     6481 gaaattcaca atagttctga aggccgattt ttacttgcct atttgaaagt cctgaaagtc
     6541 ttcaaaatta gccatggtga catccacata tgtgactttt gcccatttgg atggtccttt
     6601 acgaacttct tgaataaatg tggctatttt atctgaatct tttgattgag ccagaatttc
     6661 aacggtccca tcgttgttat tccagacacg accacaaata tctcctatat ctagagctaa
     6721 agtatgagta gcgtatcgaa aaccaacacc ttgtacacgt ccagaaacaa ttaaacgtac
     6781 tttttgcata agaagggcct ccttgctttt attatagcac atacttagca ggaaatctct
     6841 ttagctaatt gtctacttcc tgtcactttt actaaagaca agaatacaaa aatgagatag
     6901 ttagtagtac taacagtaaa agaggtttgt ctattttaaa aatagtatca aaaaaaggtc
     6961 ttttaggttt aaaacattga actgacccca aattgaccat ctaagaagtt gatgctttca
     7021 aatgctgtcg cgtatggtat aataggcgca tggttattac atcaaaagca aatcaactaa
     7081 ttaagcaaac aaaaaaatta ttgcaaaaaa agcatcgaaa acagtcctat ttgattgagg
     7141 gctggcacct ttttgaagaa gcccaaaaat caggacaagt ttttcgccac atctttgttt
     7201 tagaggaaat ggtggagcgt ctagcaggtg agcaagaact cgttattgtt agcccacaag
     7261 tgctcaagga gttgactgac tctcctagtc cacaaggcat tgttgctgag gtggagatac
     7321 ctaagttagc ctttccatca gattacaaag ggaaatattt ggttttagag gacgtccaag
     7381 acccaggaaa tctggggacc atcattcgta cagcagacgc tgcacggttt gatggggtct
     7441 ttctttctga aaaatcagca gatatttaca accaaaaaac cctacgttcc atgcaaggta
     7501 gccattttca tttaccaatt tggcgaacag atgtctatca gctttgtcga gagctccaag
     7561 agtatgaaac tcccatccta gcaacaactc tgtcaaagaa atctgttgat tataaaagcc
     7621 tcacacatca tgaaaggttg gctttggtgt tgggaaatga aggccagggc attagtgctg
     7681 aaatggcagc cctagcagac caactggttc atattacaat gcctggtcag gcggaaagtt
     7741 tgaatgttgc cgtagcagca ggaatcctca ttttcagctt gatttaagct aatatgatat
     7801 aataaccaag tgaggtaatt atgttatgca ttatacagaa gataaagaat atatggaaca
     7861 cgtcggtcat ttgatcgctc atcctcgctt tcaaaaattg agtcatattg ttcagcacca
     7921 acattcaacg cgtcttgaac attctatcaa tgtttcttat tcaagttata aattagccaa
     7981 gcgatttggt tgggatgcta aaagtacagc cagagggggc ttacttcatg atttctttta
     8041 ttatgattgg cgcgtgacca agtttaataa gggtcatgcc tgggttcatc ctcgtattgc
     8101 tgtacgaaat gcgaaaaagt taacagaact caataaaaaa gaagaggata tcattctaaa
     8161 acacatgtgg ggagcgacca ttgcttttcc gcgttacaag gaaagttata ttgtgacaat
     8221 ggtagataaa tactgggctg tcaaggaagc agtgacgcct ttgagacaaa aatggtctaa
     8281 tcgacggttt cttcgccgca agaccctcca aagtcataat cgttaagtaa aggagttatt
     8341 atgaacgatc acgttattta tacacaatca gatgtgggat taaatcagtt ttttgctaaa
     8401 atttatagtc tagtgggaat gggagtgggt ttgtctgctt ttgtttctta tttgatgctt
     8461 tatccattta gagaaaattt aatctctatt cttgttaatc agccgatgat ttactatggt
     8521 gctgcgatta ttgaacttat tttagttttt gtagccagtg gtgcagcacg aaaaaacaca
     8581 ccagctgcat tgcccatttt tcttatctat tctgcgctaa atggctttac cctaagcttt
     8641 attattgtgg catacgctca aacgacagtt tttcaagcat ttctttcttc agcagctgtg
     8701 ttttttgcca tgtcaatcat tggtgtcaag actaagcggg atatgtcagg tcttagaaag
     8761 gctatgtttg ctgctttgat tggtgttgtt gtggctagtc tgattaatct ttttattggt
     8821 agtgggatga tgagttatgt cattagtgtg atttccgtgc taattttctc aggtctcatc
     8881 gcatcagata atcaaatgat taagcgcgtt tatcaagcaa caaatggcca ggttggtgat
     8941 ggctgggctg ttgctatggc cttaagcttg tatttagatt ttattaactt atttatcagc
     9001 cttcttcgta tttttggaag aaatgattaa cattaaaaag tgagtataac tactcacttt
     9061 ttaatgttaa caggataagt aaactgtaag gaagaaattc gagatagata taagatgagg
     9121 ctgctaaaag acctagtgtt aatttgtaaa ctctttcttt ttttggtata ctattaagga
     9181 ttaaatacga ggagaatata gatagatatg tcaacagcta tttggatttt attacttatt
     9241 gttgcccttg gggtaggtgt ttttggtggg atttttattg cccgtaaaca aattgaaaaa
     9301 gaaattggtg aacacccacg tttaacacca gaagctatcc gtgaaatgat gagccagatg
     9361 gggcaaaaac caagtgaagc aaaaatccaa caaacttatc gtaatatcat caaacaatca
     9421 aaagcagccg tatccaaagg taaaaaataa aagctaattg ttatagggga aacctttgtg
     9481 atagttagaa gtgaacagtc tttgatctca atgttcagta gcacttgtct tatcagttag
     9541 atttgtcaac tgttcagggt ttaaaaagaa actcagttgg ctatttccaa ctgagttttt
     9601 catatcgtca gtaaggaagt aaacataatg gatacaagac caattgggtt tttagattct
     9661 ggtgtgggag gtctgaccgt cgtttgtgaa ttgattcgtc agctccctca tgagaagatt
     9721 gtatatattg gtgattcagc aagagccccc tatggtccta gacccaaaaa acaaattaag
     9781 gagtatactt gggagctagt caattttttg ctgactcaaa atgttaaaat gattgttttt
     9841 gcatgcaata ccgcaacagc ggtggcttgg gaagaagtaa aagcagcttt agatattcct
     9901 gttttagggg ttgtcttacc gggggcaagc gcagctatta aatcaacgac aaaaggccag
     9961 gttggggtca tcggaacccc aatgacagtg gcttcggaca tttatcgcaa aaaaatccag
    10021 ctattagcac catctgttca agtaaggagt cttgcttgcc cgaagtttgt accgattgtg
    10081 gaatcaaatg agatgtgttc gagtatagct aaaaaaatag tttatgacag tctagcacca
    10141 ttagtcggta aaatagatac ccttgtacta ggatgtactc actatccctt gttacgacca
    10201 attatccaaa atgttatggg gccatctgtt aagctgattg acagtggagc agaatgcgtc
    10261 cgagatatct ctgtcttatt aaactatttt gacattaacg gtaattatca tcaaaaagct
    10321 gtcaaacacc gcttttttac aacagcaaat cctgagattt ttcaagaaat tgcctcgatt
    10381 tggctgaaac aaaaaataaa tgtggagcat gtcaccttat gagtgaaaaa atttacgaat
    10441 acaaagatga aaataactgg tttatcggaa agatgactgg tcataattta atatcgggct
    10501 ggggagtcaa acacacgaca atcaaaaaaa tagatgacct tttagacggt attgccgcaa
    10561 cgttagattg ggaaaatcct aaaggttacg atgtttcggt ggtgcgttat cagtctcctt
    10621 tatctctcat cacctttatt atcgacatga taaatcaaga gacccagcgt gagattaagg
    10681 taactcccca tgcaggcacg attttattaa tggaaaatgc gaaactgctt gcagtttatc
    10741 tcccagaagg tggagtttca acggctacat tttttgcgac tagtgaacag ggatttgggg
    10801 atactatttt aatcgctaca cgaaacgagg gaaaaaccaa agagttccgc aatctgtttg
    10861 gccagctagg ttatcgtgtg gaaaatctca atgattatcc agaattgcca gaagtggcag
    10921 aaactggtac tacttttgaa gaaaatgctc gcttgaaagc agaaacgatt tcacgcctca
    10981 caggaaaaat ggtattagca gacgattcag gactaaaagt tgatgccttg ggtggtttac
    11041 ctggggtttg gtcagctcgt ttctcaggac cagatgccac agatgccaag aataatgcca
    11101 aactcttaca cgaattagct atggtgtttg accaaaagaa gcgctcagct caatttcata
    11161 cgactttggt agtggctgct cccaataaag atagtttggt tgtggaagct gagtggccag
    11221 gctatattgc tacgcagcct aagggagaaa atggttttgg gtatgaccct gtctttatcg
    11281 tcggagagac tgggcgtcat gctgcagaat tagaggctga ccaaaagaat cagttgtcgc
    11341 atagaggtca agcagttcga aaattaatgg aggtctttcc agcatggcaa gcaaaacaat
    11401 catagtaatg agtgactctc atggtgaccg agatatcgtt caagccatta aagacaagta
    11461 tcttggtcaa gttgatgcta ttttccataa tggggattct gaattaaaca gctcagatcc
    11521 tatttgggct ggtatttatg tcgttggcgg taattgtgac tatgatacag gttaccctga
    11581 tcgtttagta actcaattag gcacagttac gattgcccag acacatggtc acttgtacca
    11641 tattaatttc acttgggata aactggacta ctttgctcaa gaagtagcag cagatatttg
    11701 tctatatgga cacctacacc gtccagctgc ttggcaagtt gggcaaaccc tctttatgaa
    11761 tcccggatca gtaacacaac ctcgcggaga aattaacgag aaattatatg ctcgtgttga
    11821 actaactgat actcaaatta aagttgatta ctttacaaga gatcataaac tttacccatc
    11881 actgtctaag gaatttaaac gatgatcgca aaagaatttg aaacattttt aatgagtcat
    11941 ttagataatt atcttatccc ggaacaagac ttggctattt ttatcgacac acataatgca
    12001 gaccatgtga tgttactatt ggtgagcaat ggtttttcta gagttcctgt tatcacacgg
    12061 gagaaaaaat atgtgggaac cattagcatc tcagatatta tgatgtacca atctaaaagg
    12121 cagctgacag attgggaaat gagtcaaaca gatattggcg agatggttaa taccaaaatt
    12181 gaaaccatta gcattacttc tagcctaaca gaaattatgc acaagttaat tgatttccca
    12241 ttcttaccgg ttgtagatag agcgaatcgc tttgttggga ttattacgcg aaaatctata
    12301 ttaaaggctg tcaacagtct tttacatgat tttacagatg attacacgat tataaaaaaa
    12361 tgaagagtta tattgaacct tttattgcta gcaaggcttt atctcaaaat agtcaaaaag
    12421 cttatcgata cgatttgcaa cagttttgtc aactggttgg tgaacgtgtc aatcaagata
    12481 aactattact ttatcaaaat agcatagcta acctaagttt atctgcaaaa aaacgcaaat
    12541 tatcaacagc taatcaattc ttatattact tgtatcaaat aaagtattta aatagctatt
    12601 ttcgattaac agataccatg aaagcgatgc ggactgagaa gcagcaagct gctattatta
    12661 atactgatat tttctatcaa aaaacccctt ttgtgtgggg gcaattaatc agcttattaa
    12721 tattggagct gggtttaact ccgagtgaag tagctggtat tgaagtagct aacttggatt
    12781 taaattttca aatgttgacg ctaaagacta aaaaaggtgt gcgtgtatta cctttgtcgc
    12841 aaatactaat ccctttttta gaacaacaac tcgttggcaa agaagtctat ctttttgaac
    12901 atcgaggcat ccctttttca agacaatggt tttttaatca cctaaaaacc tttgttcgaa
    12961 gtattggtta tgaaggatta actgctcaaa aattacgaga gcaatttatt ttgaaagaaa
    13021 aactagctgg taaatcgata atagaactta gcgatattct tgggctgaaa agccctatga
    13081 ccttagaaaa atactacaaa tcataatgga tataaaactt aaagattttg aaggtcctct
    13141 tgatttactc ttacacttgg tatcacagta caaagttgac atctacgagg ttcctattgt
    13201 tgaggttatt gagcagtacc ttaattatat tgaaacctta caggtaatga aacttgaagt
    13261 ggctggtgac tacatgctga tggcaagtca gctcatgtta atcaaaagta gaaggctatt
    13321 accaaaggtg gtagaacaca tcgaagaaga agatttagaa caagatctgt tagaaaagat
    13381 cgaagaatat agtcgcttta aagcagttag tcaagcgtta gcgaagcaac atgaccaaag
    13441 ggcaaaatgg tattcaaaac ctaaacaaga attaattttt gaagatgcta tcttacagga
    13501 agataaaacg gtgatggatc tatttctagc tttttcaaac ataatggcag ctaaaagagc
    13561 agttttaaaa aacaaccata cagtcatcga gcgtgatgat tataagattg aagacatgat
    13621 ggcttctatt aaacagcgac tggaaaaaga aaacgttatt agcttatcag cgatttttga
    13681 ggaatgccaa acattaaatg aagttatcag tatattttta gctagtctag aattgattaa
    13741 gcttcacgtg gtatttgtag agcagctctc taattttggg gctatcatac tacgaaagga
    13801 gaagaaatga cgtatttgtc acagatagaa gcgctgctat ttgttgcagg tgaagaagga
    13861 ttaagtttgc gacatctagc gtctatgtta tcattaacgc caacagctct tcaacagcaa
    13921 ttagaaaaac tctctcaaaa gtatgaaaaa gatcaacatt ctagtctttg cttgatagaa
    13981 acagctaata cctacaggct agtaactaaa gaaggttttg cagaactctt gcgtgcttat
    14041 gccaaaacac caatgaacca aagtttatct cgtgcgagtt tagaggtctt gtctattgtt
    14101 gcatacaaac aacctattac acgaatagaa attgatgaca ttcgaggggt aaactccagt
    14161 ggagctttga gtaaattatt ggcatttgat ctcattagag aagctggcaa aaaagatgtg
    14221 gttgggcgtc ctcatttgta tgcgacaaca gattattttc tggactatat gggaattaat
    14281 catttagacg aattgattga agtatctgcc gttgaaccag cagatgaaga gattgctctt
    14341 tttaggacac aagactgagt tttgcttgtc atcaaatagc aaatggcgtt tacaaaagga
    14401 tttgttttaa ggcgttttag ttgaaaaaaa gccttaaact agggacaaca aaaagttgct
    14461 caaaagaaag aagaatataa tgcgaattaa caaatacatt gcccatgcgg gtattgccag
    14521 tcgtcgtaag gctgaagaat tgattaagca aggcttagta accttaaatg gtcaggttat
    14581 tacggattta gcaaccactg taaaatcggg agacgtggtt gaaatagaag gaagtcctat
    14641 ttacaacgag gaaaaggttt actatttgct taataaacct cgtggcgcta tctcaagtgt
    14701 gtcagatgac aaaggccgta aaacggtact ggatttacta cctcaggtta aagaacgcat
    14761 ataccctgtt ggacgattgg attgggacac atcaggggtc cttattttaa cgaatgatgg
    14821 tgattttaca gatactatga ttcatcctag aaatgaaata gacaaggttt atctggcgcg
    14881 tgtcaaaggt attgcaacga aagaaaatct tcgtccctta actcgcggaa ttgttattga
    14941 tggtaaaaag accaaaccag ctcgctacaa tattgttcgt gtagaagctg ataagaatcg
    15001 atcgatcgta gaattgacta tccatgaagg gcgaaaccat caagtgaaaa aaatgtttga
    15061 gtctgtgggg cttctggttg ataaattatc tcgtacacgt tttgggaccg tcgatttaaa
    15121 gggactaaga ccaggagaag caagacgctt aaataaaaaa gaaataagtc aactgcataa
    15181 cttagctaat acgaaaaaat gatgaaaaaa ctattaatag tatctgttaa agcttatcaa
    15241 aaatacattt ctcctttatc tcctcctagt tgtcgctaca agccaacctg ttcagcttat
    15301 atgttaactg ctattgaaaa acatggtact aagggaatac taatggggat agcaagaata
    15361 ttaagatgtc acccttttgt tgctggtggt gtcgatcctg ttccagaaga ttttagccta
    15421 atgagaaata aaaacacgtc taaaaacgct gaaaaggcat gactttatca aaaaatggct
    15481 tgacattgat agctgaagtg ttataatgga aaacaagcat tcgttggttt aaatcaaacc
    15541 tgttatgatt taagttagcg agtcaccatc aaccacattg tttgctgagc atgactccga
    15601 gcagtgtggt tttttaaatt aaaaagagta aatgagatta tgacgacaaa agaacttatc
    15661 aacaaaaacg acaaagtaaa aaaggctcgc aatcatattg tcctgtttca accccaaatt
    15721 ccacaaaata ctggtaatat tgctagaaca tgtgctgcaa ctaatgcacc tttacacatt
    15781 attaaaccca tgggatttcc tattgatgac cgcaaaatga agcgtgccgg cttggattac
    15841 tgggacaagt tagaactgca tttttatgat catttagaac agtttatcaa tcaatgtcac
    15901 ggacagcttc acctgattag caagtttgca gttaataatt attctcaagc tacttatgct
    15961 gatggcgatt cccattattt cttgtttgga cgagaagata caggcctgcc agaagatttt
    16021 atgcgagaac atgctgaaaa agcacttcgt attccgatga atgatgagca tgtacggagt
    16081 ttaaatgtct caaatacggt ttgtatggtg atttatgaag cccttagaca acagggcttt
    16141 caagggttag agttaaagca tacttacgaa cacgataaac taaaataatg gactttttaa
    16201 aagcgtcagt ttttggcaac atgcgttgtc agtagctgac gcttttaaaa atttagtcaa
    16261 agctattgct atctaggttt aaatctgcta tgctagaagt gtcttcaggg cagggtgtga
    16321 ttcccgaccg gcggtgacta ggtaactaga agtccgcgag cgcaagctga tgtggtgtaa
    16381 ctccacaacc gacagtatag tctggatgag agaagaccgg gctttttgtg taggcttatt
    16441 ttagctacac aataacgacg gaacttcttt caatttgaaa aaattggagg aattttttaa
    16501 tgtcaaaaac acataaaatg attatgattg gtatactttc tgcgatatca tttctattga
    16561 tgctagttag ctttgccatt attccaggcg cagctttttt aaaaattgaa tttagtatta
    16621 ttcctgtttt atttggttta atgattatgg acttaaagag tgcttacttg attttgttat
    16681 tgaggtcctt attgaaactc tttttgaaca atcgtggggt taatgatttt attggtctcc
    16741 caatgaatat tatcgctatc gctttatttg tgacggcttt tgccttagtt tggaaccgtc
    16801 aaaaaacgct tagtcaatat gtatttgcta gcttactagg cacagggtta ttaacgtttg
    16861 gcatggttgt tcttaattat acttttgcca ttcctttata tgctatattt gcaaatattg
    16921 atatcagagc ttatattggt gttactaagt atatgatgac tatggttatt ccgtttaatc
    16981 ttgttgaggg gttgatattt gcaattacct tttattttgt gtatattgca agtaaaccaa
    17041 ttttagaaag atacttacac taatatgatg acaaacaaac aaactcactt tttaatagct
    17101 tcttttgcat tactaatctt tgttataata ggctatacgg tcaaattttt tcctgaacgt
    17161 ttggcacttc tagataatac tattcaagca gagattagag gcaaccttcc tatagtgctg
    17221 acgcaattct ttaggggtgt tactgtattt ggaaatgtca tgacacaagt tttgttagtt
    17281 attgtgtcgg tcctagtttt attctttatg aagtggaaaa ttgaagcctt gtttatctta
    17341 tcaaatggtg ccattgcagc atttttgatt acgactctca agctctttta ccaaagacca
    17401 agacctgcta ttgagcattt ggtttatgct ggaggctatt cgtttcccag tggccatgcg
    17461 atgggtagca tgctgatttt tggtagccta ttgattattt gttatcaaag gctacattca
    17521 aaactattac aatttgtgac tagcatgatc tttataatac taattttagt aattgggtta
    17581 tctcgtattt atctaggggt acactaccca agtgatattt tagcaggctt tgtattagga
    17641 tttgggattt tgcagtttat ttatcctttt tataagcaaa aacgatttga atggcgattt
    17701 cttttgaaac aaaactaatt tttcttgaga agacataggg cttatttaaa tcatttagaa
    17761 taacattggc aagcatcacc ccttgattgc cagtgttatt ttagttttag ttagatgatg
    17821 tgagctgatg attagtaaat acgtttagat aaaaaatgat taaagtgcta tgtaacgtca
    17881 gaaaagacgt gatttttgtt gatctgtaat cattaagtaa gctgatgtag aagtagtgaa
    17941 ttctaatgac tgagctatct tttgcataga tgactatctt gctagtaatg atttgtgcta
    18001 caatagaaac atgaaaaaaa gatatcagac attaaacgag cattaccgtc aactatttgg
    18061 cgcaaaaatg tttaaagttc cgattgatgc cggctttgat tgtcccaatc gtgatggaac
    18121 ggtagctcat ggaggatgta ctttttgcac agtatcaggt tctggagatg ctattgtagc
    18181 accagatgct ccaattaaag agcaatttta taaagaaatt gattttatgc accgtaaatg
    18241 gcctgatgtc aatcgctatt tggtttattt tcaaaacttt accaatactc atgatacagt
    18301 tgatgtgatt cgggaccgtt acgaacaagc tattaacgaa cctggagttg ttggtattaa
    18361 tattggcaca cggcctgact gtctaccaga tgataccatt gcttacttag cagaattatc
    18421 agaacgcatg catgtgacag tggagctagg cttacagaca acttatgaag aaacttctag
    18481 gctaattaac agagcacatt cttatgattt gtataaagaa acggtgaggc gactaaggca
    18541 ttaccccaac attaatattg tctcgcactt gattaatgga ttgccaaaag aaacccatga
    18601 catgatgctt gaaaatgtga gacgctgtgt aacagacaat gacattcaag gaattaaatt
    18661 gcatttactg catttgatga tcaatactag aatgcaaaga gattaccatg aaggacgctt
    18721 aaagttgttg agtcaaaagg actatgtttc gattatttgt gaccagctag agattatccc
    18781 taagcatatt gtgattcacc gtattacagg agatgcccca cgagatatgt tgattgggcc
    18841 catgtggagt ctaaataagt gggaagtatt gaatgccatt gataaagaaa tggaaagacg
    18901 aggaagcttt cagggctgta aagttgacta atttgtctca ttttgacgga tttggatagg
    18961 agaaaagtga tgctaaaacg ccccattcat ctctcgcatg attttttagc ggaggttgtg
    19021 gataagtcta gtgtggttgt tgatgccacg atgggtaatg gaaatgatac tgcctttttg
    19081 gcacaattag ctaaaaaggt ttatgctttt gacgtgcaag aacaagctat tagaaaaaca
    19141 agtgagcggt tagcgcagct aggattatcg aacgctgagc tgattttagc gggtcatgaa
    19201 gcagttgatc agtatgtgac ggagccggtg cgtgcagcga tttttaacct tggttatttg
    19261 cccagcgcag ataaatctat tattacctta ccaaatacca cactccaagc tttatcaaaa
    19321 ctactaacgt tattaatggt aggtggacgt attgctatta tggtctatta tgggcatgat
    19381 ggaggcagtt tggaaaaaga tgctttatta gattttgtca agcaattaga ccaaagaaag
    19441 gtttctgcaa tgctttatca gcctcttaat caggtgaata ccccaccttt tttaatcatg
    19501 ttggagaaat tagcagacgt ttagccctta tccctgtgtc aagtctacta gaagttctgc
    19561 gattatttcg gtggaaaaag aagaaattcc ataggctttg gctataaatg tgatataatg
    19621 aagtcactat attatttttt taggagttac attaacatgg aagaccctgt gagtcagccc
    19681 ttagtgattc aatttttatt gttagttgtt ttaaccttgt taaatgcttt tttttcagcc
    19741 agtgaaatgg ccttagtttc tctcaatcgt tctcgggtgg aacaaaaagc agcagacggt
    19801 gataaaaaat acgctcgttt gttgcgggtt ttagaggaac ctaatcattt tttatcaacg
    19861 attcaagttg ggattacctt tattagttta ctatcaggag caagtttatc agcttctttg
    19921 ggtaaggtga tctcaggttg gctaggtaat tcagcgactg caaggacagc tggtactatc
    19981 atctccttgg ttttcttgac ttatgtctct attgttttag gagaattgta tccaaaacgg
    20041 attgccatga acctcaaaga caagttggcg attgtttcag cccctattat cattgggtta
    20101 gggagactgg ttagtccctt tgtatggctc ttatcagctt ctactaattt actgagccga
    20161 cttaccccta tgacctttga tgatgcagat gagcaaatga cacgtgatga aatcgagtat
    20221 atgttatcaa aaagtgaggc gacccttgat gctgaagaaa ttgagatgtt gcaaggagtt
    20281 ttctcacttg atgaaatgat ggcgcgtgaa gtcatggtcc caaggaccga tgctttcatg
    20341 attgacatta acgatgatcc gcttgaaaat attcaggaaa tcttaaaaca aagtttttca
    20401 cgcattcctg tttatgatgt ggataaagat aaaattatcg gtctcatcca cactaagcgt
    20461 ctcttggagt caggtttccg ccagggattt gatcagatta acatgcgaaa aatgttacaa
    20521 gaacctcttt ttgttcccga aaccattttt gtagatgatc tcttacgcca gctgcgcaat
    20581 acccaaaatc agatggctat tttgctagat gaatatggtg gtgtggcagg acttgtgact
    20641 ttggaagact tgcttgaaga aatcgtcggt gaaatcgatg atgaaaccga taaagcagaa
    20701 caatttgttc atgagattgg agacaatacc tatattgttg ttggtaccat gactttaaat
    20761 gagtttaatg actattttga taccgaacta gaatcagatg atgtagatac cattgctggt
    20821 ttttatttga caggtatcgg aaccattcca agccaggagc aaaaagaagc ctacgaaata
    20881 gataacaaag acaaacattt agttctaatc aacgataaag tcaaagatgg ccgtatcacg
    20941 aaattaaaat taatcctgtc taatatagaa cagattattg aggaagacta ggctccatgc
    21001 ctagtctttt tgatgaaaag tgttataatg aaaatgtaaa aacgtttaca agtaatgaag
    21061 gagatgcaat gactgagaaa gattatggac aagtaacagg gatggtgcat tcgacagaaa
    21121 gttttggttc tgtagatggt ccaggcattc gttttatcat tttcttgcaa ggctgtaaat
    21181 tacgttgcca atattgccat aatccggata cttgggagat ggaaaccaac aattcaaaaa
    21241 tcagaacagt caatgatgtc ttgaaagaag cccttcagta caaacatttc tggggaaaaa
    21301 aaggaggaat taccgtatct ggtggtgaag ctatgcttca aattgatttt atcactgcct
    21361 tattcatcga agctaaaaaa ttaggtatcc ataccacact agacacctgt ggctttacct
    21421 atagaccgac acctgaatac catcaggttc ttgataactt attggcagtg accgatttga
    21481 ttttattgga cttaaaagaa attgatgaaa aacaacataa aattgtcacg cgtcagccta
    21541 ataaaaacat attgcaattt gctaggtatt tatccgacaa gcagattcct gtctggattc
    21601 gacatgtctt ggttccaggc ttgactgata ttgatgatca tttgacacga ttgggtgagt
    21661 ttgtcaaaac cttgaaaaat gtcgataaat ttgaagtttt gccttaccat accatgggag
    21721 aattcaagtg gcgtgagcta ggtattcctt atcagttaga aggtgttaaa ccaccaacta
    21781 aggaacgcgt tcaaaatgct aaaaatctga tgcagaccga atcttacaca gaatatatga
    21841 atcggattca tcagtcataa gagctgttat agggttgagg ttgcctcaac tttttaaagt
    21901 attataagca gatcttttgt aatcttcctt tgattttagg gggtaatttt gctaaaataa
    21961 aatgaataaa caaaatgagg tagaatgatt atgtcaaaaa ttttagtttt tggtcatcaa
    22021 aatcctgata cggatgctat tgcatcatct tatgcttttg attacttatc ccaaaaagcg
    22081 tttggtttgg atactgaagt cgtagcttta ggaacaccta acgaagaaac agcttttgcc
    22141 cttgattatt ttggtgttga ggcaccacgt gtggtagagt ctgctaaagc acaagggtct
    22201 gaacaagtga ttttgacaga ccacaatgaa ttccaacaat ccattgcaga tatccgtgag
    22261 gttgaggtat atggtgtggt tgatcaccac cgtgtggcta attttgaaac tgcaaatcct
    22321 ttatacatgc gtgtggaacc agtaggatca gcctcatcta ttgtttaccg tatgtttaaa
    22381 gaaaatggca tcgaggtgcc aaaagcgatt gcagggatgc tcttgtctgg tttgatttca
    22441 gataccttgt tgttgaaatc tccaacaact catgtgtctg atcaccttgt cgctgaagaa
    22501 ttggcagagc tagcagaagt gaacttggaa gactatggaa tggctctttt aaaagcaggt
    22561 acaaaccttg caagcaaatc agaagtagaa ttgattggta ttgatgccaa aacatttgaa
    22621 ttaaatggca atgctgtgcg cgtggcacaa gtgaacactg ttgacatttc agaagtctta
    22681 gaacgtcaag aagctattga agcagccatt aaagatgcca tggctgcaga aggttattca
    22741 gactttgttt tgatgatcac ggatattgtt aactcaaact ctgaaattct agctattgga
    22801 gcaaacatgg acaaggttga agcagccttt aacttcaccc ttgacaacaa tcatgccttc
    22861 ttagcaggtg ccgtttctcg taaaaaacaa gtggttccac aattgacaga aagttttgga
    22921 gcttaatgaa aagacgcttt aaaaggactt ggtttttttg accaagtcct tttttcactc
    22981 ttctatgcta taatagaagg tatgataaca atatttcatt cagataaact caccagacaa
    23041 cctttctttc aagaccttat caattatctc gaccagcatg atcacgttat attacgagag
    23101 atcaaaaaag cctttcctaa tgtgacaggt attgacaagg ccatcgaaag ctatgttcaa
    23161 gctggctata ttcgccgtga aaataagcgt tatggcatca accttccttt ggtgagttct
    23221 gatcagcagc tggccttaga cactatgctt tttgtgaaca cctgttcagc tatgtatgaa
    23281 aatattttag cggttgtttt tgagactcag ctaacaaacc aaaccaatca cgtgatgatc
    23341 aaggaaaaga ccaacatcac gagagacgat ttgaccctgg ctaattattt ttaccgtctc
    23401 aaaagaggtg agaagccatc agctgagcag atggatttgt atgacctctt gggggatgtg
    23461 aatcaggaat atgcccttaa atatatgaca acttttttgc ttaaattcac gcgcaaagac
    23521 cttgtgatgc aaaaacgtcc tgatatattt gtggaagctc tggttacact tggttaccta
    23581 aaacaagtgg agcctacaac ataccagctg ctaatgacct tggataaaga aagtttaacc
    23641 tttatagcac catagaaaag agctaggaac aaggactgtt cttagctttt tgagtgcttt
    23701 tattttgttt ttgccattaa gtaaagaaaa taaggggctc cgataagcga cactaaaatc
    23761 cctgtctgga tattactgcc aaccagatag actcgtccta ctgtatctgc aacaagaagt
    23821 aggatcattc cgattaaaat actaatgggt attgttattt tatgattact ccctaccaag
    23881 tatgtactaa agtgtccagc tattaagccg ataaaactaa tattaccaac caaaagaaca
    23941 ctcaaagcac ctaagccagc tgctaagaca agtatcaaac gacgcttacg gtttaaaggg
    24001 agtcctagcc caatagcagt attatcagct aatcccataa tattaaggaa atgggcttga
    24061 ctataagtca atagccaaaa acacaataat aaaggagcga tgacactcag agtaggccag
    24121 tcgtctccta ttaattggcc gctaagccaa ttgatgacca aatcgacttt gtagcgatta
    24181 atatgaccaa caagagcaac cattagactt gaaagcatgg tagtaacagc tacaccagta
    24241 agaattaacc gtgtaggatc aatttgtcct tgttttgtca aagatagcca ataaacgcta
    24301 aaggttgtta caacagcacc aagcatggca aataaaggca agtaatggct gatatgagct
    24361 tgctcaaaga aagaaaagga gaccacaata gccaaagaga tacctgctcc tatagtaata
    24421 cctaatacac cagagtcagc taagggattt cgtgttaaac gttgtaataa tagccctgat
    24481 aaagcaagag aaccgcctcc tagacaagca gctagaattc ttggcagacg aatgttaata
    24541 acgatgaaag atatggcatg gctagatttt cccaaaaaca catggataag gtccaaaaaa
    24601 gatagatgag attctccaag ggacaaagct aatcccataa gacttataag tagcaggatc
    24661 aggctagtat ataaccttaa aaggtgtcgg tgagatttca atagcggcct cctcttctta
    24721 ttaaccataa aaagcatgga aaaccaatta agctaaccaa tgccccaaga ggggtttcat
    24781 aaggcggatt gagattacga caagcaatat caacccatac catgaaacta gcgccagaaa
    24841 ctgcacaaag cggcaaaagg tatcggtaat gatgaggtgt aaagtgtttc ataagatgag
    24901 gaatgactaa accgataaaa gaaatgcttc cagcaatggc tacagctgct gatgataaaa
    24961 taagaaccaa aatcataaag actgcactga tcaagttggt cttttgccca agagcttttg
    25021 cctgagactc acttagacta aggacggtca ggtgataaga taatagctgg gctaaacaaa
    25081 gactaagaat aataagagga gcaatatagc cgatcatttg ccaattgacc ccgacaagac
    25141 ctcctgcttg ccagccgata acagcatttg ccagatgata gtaattggta ataccttgac
    25201 ctaatgctga tagcagtatg gaaaccatag ctcctgctaa gacaaggcgg agctgatggt
    25261 agcctttgcc agattgataa gaaaggccaa agactaaggt tgctgccagg ctagacccta
    25321 acaacgaaag cagaataatg agggaatagt gcaagtgagg tacaaacgca taagccagta
    25381 tcaaggctag tcctgctcca gcattaatgc ctaaaaggcc aggctcagcg ataggattac
    25441 gtgtgattgc ttgcatgata gttccagaaa cagctaacgc agagccagtt aaggtagcac
    25501 caaaaagtct cggtaatcgt atagcgagta aaacattggc tttatggccg ttttgctttc
    25561 caaataaaat agagttaaga tcctgatgtg ataaagcaac ggctccaaag cgcagatgac
    25621 agtaaatagc aacacataag ctaaaactaa gtagcacaaa gacaagccaa aagatatgtg
    25681 acttagtgat tatctgcgtt ttttttaagc aaaaacgctc gagacacaaa aagggatgct
    25741 tagttttcac ttgataagat tgcctctcgt aaagtctcta attgatattc taaagaaaga
    25801 ggatcagtaa agtaaaaaac attggcattg actttgatca catggtgttt tttcacagct
    25861 ggaatggatt gccatagctt gctttcatat agagcagaac cagtcttatc atcttctgca
    25921 gcaacgacga cataatcacc aatatagtct ggcaagactt cctgcgacaa tgacaggtaa
    25981 ccttgtttaa aaacttcagt cttcactttt tcaggagcat cataatggaa agcttgatgg
    26041 ataatctctc cgccacgtcc ccagtcttta ccaaaaaggt aaacgtcttt ttcataaagc
    26101 cccataatag taaaggttgc tttgtcacct gtaacggcct tgacttcttt ttcataagct
    26161 gctgttttag ttttccaatc ttttaaccat ttcttggctt tgccttcttt gttaaagatg
    26221 cggccgaagt cagataacac ttgtaaataa tcacgtttgc ggtattcaat cgaaataaca
    26281 ggcgcaattt ctgccaattg tttaatattt tcttctgtag agccaacaac aatgaggtca
    26341 ggcttaagcg ttgtaatggc ttctaaatca gttgcagcaa cttgctttgc tttttttaca
    26401 gtctttgcta agatggggtt tttcttgtca taagaagtaa ccccgactag attcatatct
    26461 aattttttga ggtaaccagt gtaagttgaa gccagactga ctactcgctt aggctgttta
    26521 ggaatatctc cgtaataagt cacaccagca atttgaggca taggcgataa actttttgag
    26581 gcggtgttag aatgattagt tgcttgatta ccacaggcga ttaaggtaat agttgtcaaa
    26641 cagagtgtta atagtagagt taattttttc atgtatgcta attctcctag aataaagtta
    26701 ttgaagctga taagtcagca ctataggaca gttgtggata ggatcatcaa ccagtaccgg
    26761 tttgatttgg aagatgtctt gaatgatagg tgaagtcata acatctgcta tagtaccagt
    26821 ataatggatc ttgccatgtt tcatagcaat cagatgatcc gaatagcggg ctgaaagatt
    26881 gaggtcgtga agaaccataa caatagtctt gccggcatcc ttatttaggg atttaacgag
    26941 ctctaggatt tctagttgat gatttaaatc taaataagta gttggctcat ctaaaaaaat
    27001 agtatcagtg ccttgggcta atgccattgc aagccaaacc ctttgacgtt gccccccaga
    27061 aagagcatca acaggttgat cagcataggc catgacattg gtggcttgca tagcccaatg
    27121 aatggctttt ttgtcagctg gactaagatt tccaaagtaa gattgatggg gaaaacgtcc
    27181 gtaagaaacc aattcgtaaa cagttatgcc gttagttgct tcttgaactt gaggtaaaag
    27241 agctagtttt ttagcaacct ctttagtttc taatgtggca atgttttgcc catttaaata
    27301 cacaactccc tgttttggtg gtaataatct tgtcagtgct tttaataaac tagacttccc
    27361 acagccattg gcgccaataa tggtcgtaat tttaccttca ggaatataaa acgataattt
    27421 atcgatgatg gtacgctgtt cataggcaat tgtgaggtct tcagcactaa ttgttgtcat
    27481 atggttgcct cctttctaaa aagagtcaaa gggttgatga aagtgcatat taaagtattc
    27541 atctgcccat tttaaaatag ttgagaagta gcttattttc tcaaaatctt cactattata
    27601 acatagaaaa aaggcttaac aaagcctttt ttgatgtgat ttttaggatg tattaaaaaa
    27661 gaaatattag tttagaatga ttaaaaacaa tagcgataat gattctaaac taacgtcaac
    27721 atactctaga ggtagtgttt ggcgattgct aaatcaccag cataagctac ttgttgtcct
    27781 ttaacaattt gataagcatc tgcgcctttc ccagcgataa tcacagcatc ttttgctcct
    27841 tggcaaagag acatggcttt ttgaatagct tgctcacggt cactaataat ctctaccggg
    27901 cgtgcgatat ggctagcaat ttcttttgaa atatcttctg gatcctcgaa attagggtca
    27961 tcagcagtga gaatgacggt gaggtttgga tgctgatgaa tcaccctacc aaaatcagct
    28021 cgtctgctct cgcctttatt tcctggtgca cctaaaatca acattaattt gcctgtctga
    28081 tgttcttcca caacgctgag taatttttct aagctgtcac cattatgggc ataatcaaca
    28141 aaaactttag catgattagt catggtgagg acttccatac ggcctgggac gcgagtctta
    28201 gcgatacctt tttgaatatc agctaggcta gcacccaaac gaaggcaggc aagtccagct
    28261 gccatagcat tttcttggtt aaaatggcca attaattgaa tgtcataatg gccagctaat
    28321 tgtcctttag cttcaaagga gaaggcttgg ctagtggtga tctggttgtc agacaaggga
    28381 ccataaaaca catgctcttg atccgctacc tgatccgcta gaaatgaaaa atgatccatc
    28441 ccgctattga tcgcaacagc tctgctattc tccattaaaa gacgcttatg atagaaataa
    28501 tcttcaaacg ttggatgctc gattgggcca atatgatcag gactaatatt taggaaaacc
    28561 ccaacatcga acgtaagccc atagacacgg tcaacgaggt aagcctgact agaaacttcc
    28621 ataatcaagt gggtcatgcc attagtcaca cactcagcca tcatagcaaa aagatccaag
    28681 ctctcaggag ttgtgagttg tgatttgaaa aaggtcttgc catctaaggt ggtattcatc
    28741 gtagacaaca aggcaggttt ataggattct ttcaaaatat ggtaggcaaa ataagcagct
    28801 gttgttttgc cttttgttcc agtaaaccct aagagcttga gctgatcctg aggattacca
    28861 tagaaagcca tggcaattag actcatagct tttttgatat cagtgaccaa aacaacggga
    28921 ataccaagct cgtaatctac ctctgaaata tagagttgta atccgttagt gattgcttct
    28981 ttaaggtagt cagctttgaa agttgcccct ttagcaaaaa aaagggtctt gccatctact
    29041 tgacggctat cgtaactcaa gcgctcaaaa ctaaagcctt gataatggta gtggtatccg
    29101 tcggcatcaa gaacctcacg aaaattatgg tcttttttta aaatgtctaa taattgttca
    29161 atggttatca tacccttatt ttatccttaa agttcttgtt ttacaagtca caagcaaaag
    29221 tttataataa agctaataga aactaggaaa gaatagatat gtcgacagaa aaaaaacaac
    29281 tgacacaaga agaattgatg gttcagggag cagcctggtc cacagctggt aatttcatta
    29341 gccgactttt gggagtgtta tacattattc cctggtatat ttggatgggg caatacgcga
    29401 ttcaagccaa cgctcttttc aatatggggt ataatgttta tgcttatttt ttattgattt
    29461 caaccacagg actcaatgtt gcgattgcta agcaggttgc caagtacaat tccatgggac
    29521 aaactgagca tagctatcaa ttaatccgaa gtaccctaaa actcatgctt ggtctgggcc
    29581 ttattttttc agccattatg tatctgggct ctcctttatt tgcgagctta tctggtggtg
    29641 atgacacact tgttcccatt atgcacagcc tttctttagc agtttttatt tttcctgtaa
    29701 tgagtgtgat tcgaggcatc tttcaaggcc acaataatat caagccttat gcagttagtc
    29761 agattgctga gcaactcatt cgggttatct ggatgctttt gaccaccttt tttatcatga
    29821 agttgggttc tggtgattac gcttcagcag tgactcagtc aacttttgca gcctttatcg
    29881 ggatggtggc tagtatgggt gttctggggt actatctttg gaaacaggga cttctagcag
    29941 ctatttttag taagccagat catactgttt ctattgatat caagggcttg ttgcttgaaa
    30001 ccttaaaaga atccatcccc tttatcgtaa caggaagtgc cattcaagcc tttcaattga
    30061 ttgatcaatg gacttttgtc aatacgatga ctctttttac agattatagc cgttctcaat
    30121 tgcttgtttt atttggttac tttaacgcaa acccagccaa aattacaatg gttttaattg
    30181 cagtagcagc atctattggt ggtgtaggca ttgccttatt aactgaaaat tatgtcaaaa
    30241 aagatatgaa agcagctgct cgcttaatca ttaacaacat tgaaatgtta gtaatgtttt
    30301 tgttacccgc tcttactggg gcaattattt tagcaagacc tctatattct gttttttacg
    30361 gagctagcga ggatcgtgcc attcacctct ttgtggcggt cctcttccaa accttgttga
    30421 tggcgcttta cacccttttt tcaccgatgc ttcaagctct ttttgaaaat cgaaaagcga
    30481 tttactactt tgcctatggt atcttgatta agttagtttt acagataccg cttatttatt
    30541 tgctacatgc ttatggtcct ttactagcga cgacgattgc tttagtggtg ccgatttatt
    30601 tgatgtatcg acgcctacat caggttactc attttaaccg caaactgttg caaaaacgtt
    30661 tattattaac cttgattgaa accttattaa tgggactggt cgtgtttgtg gccaactggc
    30721 tattgggcta tgtctttaaa ccgacaggcc gcttgaccag ccttctttac ctcctcatta
    30781 ttggtggctt gggaatgacg gtttacacgg cactcacctt gctgacgcat caactggata
    30841 aattaattgg tagcaaagct agtcgccttc gtcagaaatt aggctggcat taatataatg
    30901 acaccaagaa cgatttcgtg agtgcttatc gaaatcgttt ttttcttaat gatctcgctt
    30961 agtaaaggta gccgataaaa aatcaggatt tcccaagcaa actactgata tttttggtat
    31021 aatgataaaa gtgtaaaaaa atagaaggaa gtatatcaac atgggaaaat gtcaagttat
    31081 ttcgcatccg cttatccaac acaaactttc aatcttacgt cgtcaaacca catcaactaa
    31141 agattttcgt gagttggtta atgaaattgc gatgctgatg ggatatgaag tgtcacgcga
    31201 ccttccctta gaagatgtcg acatccaaac gccagtctca aaaacggtcc aaaaacagtt
    31261 agctggtaaa aaattagcta ttgttccgat tcttcgtgct ggtattggga tggttgatgg
    31321 cttgttaagt ttggtaccag ctgcaaaagt tggccatatc ggcatgtacc gtaatgaaga
    31381 aactcttgaa cctgttgaat acctcgttaa actaccagaa gatattaacc aacgacaaat
    31441 tttccttgtt gatccaatgt tggcaacagg tggttcggct atcttagcag tggattcgtt
    31501 gaaaaaacgt ggtgcagcaa atattaagtt tgtctgttta gttgcagcac cagaaggggt
    31561 gaaaaaactt caagaagctc atccagatat tgatatcttc acagcagcac ttgatgatca
    31621 tttaaatgaa catggctata tcgtgccagg cttgggcgat gctggtgatc gtttatttgg
    31681 gacaaaataa aagagacaac catcaaatag tagccttttt aggccaatcg ttcttgattt
    31741 tgtttaaaaa atcagagaga tttctcttct ttaaaggaaa agcaagttta tgatttgacc
    31801 tttattgacc aaaatattat aataaagaca atcagtaaaa aagagatgac ctagtgggtt
    31861 gccgtctctc aaaatgacgc tactaaaaac ccttgtagat tggagaatgt attatgattc
    31921 ctgttgttat tgaacaaact agccgtggag aacgttctta tgacatttat tcacgtttat
    31981 taaaagaccg tattatcatg ttaacagggc ctgtcgaaga caacatggcc aactctgtca
    32041 tagcgcagct cttattcctt gacgcgcaag acaacacgaa agatatctac ctatatgtca
    32101 acacaccagg tggctctgtc tcagcaggct tggctattgt cgataccatg aactttatca
    32161 aggccgatgt gcaaactatt gtcatgggta tggcagcttc tatggggaca gtcattgctt
    32221 catcaggaac aaaaggcaaa cgctttatgt taccaaatgc agagtatatg atccaccagc
    32281 caatgggtgg cacaggcggt ggcacgcaac aaacggatat ggctatcgca gcagaacatc
    32341 tcttaaaaac acgtcaccgt ttagaaaaaa tcttagcaca aaatgctgga aaaacgatta
    32401 aacaaattca taaagatgct gagcgtgatt attggatgag tgctgaagaa accttggctt
    32461 acggtttcat tgatgaaatc atggaaaaca acgaactaaa ataaagagga tctctgcaaa
    32521 agtattctag aaatttctag gatgcttttt ctcttagaaa ggaaaataat gatacagcct
    32581 actgcattaa aacagcttca agagttaaaa cgtcagttca gatccctttg caaccaatta
    32641 tgagatagtt tgaggatgat ccttagcagc tgtgtgcaag aagttatgac gatttcataa
    32701 aggatgacct agccagtctt tcttcgtctc ttttacttaa agtgtggtat aatcagatta
    32761 tgctagccaa aactaaaaaa tgcattgcca ctaaaggtgt tgtagatcgg cttatcctag
    32821 atctcataac aatgctgtag taccaccctt accttgctac taaaggcgct gttgaagcac
    32881 aaattgactt gcaaaggatc ttgactgata atatgtccgg tggaatcgcc aaagcagcaa
    32941 aaagaccaag acccttattt ggtctctacc aaaatataat agaaaaggat ttagaatgtt
    33001 tcaaaaacaa gaacgtattg gactcgtcgt ttacctgtat tataaccgag atgccagaaa
    33061 gttatcaaaa tttggtgatt tatattacca ttccaagcgc tctcgttacc tcattattta
    33121 tattaataaa aatgatttag acacaaaatt agaagaaatg agacgtttga aatgtgttaa
    33181 agacattaga ccatcagctt ttgatgatat tgaccgtcaa tttgtaggta accttcatcg
    33241 cgacgaaaca aacaaccatc aaaagggtta acaaccccct agttactgat tagcgtttca
    33301 gatttagtta gcttattaaa gtaagaaggt tttgacagat gctcacacta cttcaagaca
    33361 atgagacaac tagagcatta gtgaataact gagttgacgg tcacttcttt aataagaacg
    33421 gataggattg gtcctaaaat agagcactgc aaagatatgc cagtgctttt tagctcattt
    33481 tttgataaaa tagaactact aaaaagtgaa ggaaataaga tgatcacagg aaaactcata
    33541 accgttgaag gtccagatgg cgcagggaaa acgactgttt tagagcaact cattccactg
    33601 ttaaaacaaa aagtggctca ggagatcttg acaacaagag agcctggagg tattgccatt
    33661 tcagaataca ttagagaact tattttagac atcaaccata ctactatgga ccctaaaaca
    33721 gaactcttac tttatattgc ggctcgaagg cagcatttgg tggaaaaggt actaccagct
    33781 cttgaagcag gccagttggt ttttattgat cgtttcatcg atagttcagt agcttatcaa
    33841 ggtgcgggac gcggtttgac caaagcagac attcagtggc tcaacgagtt cgcaacagat
    33901 ggtttagagc ctgacttgac cctttatttt gatgttcctt ctgaaattgg tttagcacgg
    33961 attaacgcga accaacaaag agaagtcaat cgtttagatt tggaaacaat agagatacat
    34021 caacgagtac gaaaaggata cctagcctta gctaaggagc atccaaaacg tattgtcaca
    34081 attgatgcga ctaaaccatt gaaagaagtt gtctctgtcg ctttggaaca tgtcttagcc
    34141 ttgcttttag cctaggataa gggagggtag gaatggattt agcgcaaaaa gctcctaacg
    34201 tttatcaagc ttttcagacg attttaaaga aagaccgtct gaatcatgct tatctttttt
    34261 cgggtgattt tgctaatgaa gacatggttc tttttttagc taaggtcatc ttttgtgaac
    34321 agaaaaagga tcagacgccc tgcgggcatt gtcgctcttg tcaattgatt gaacaaggag
    34381 attttgccga tgtgacggta ttggaaccaa cagggcaagt gattaaaacg gatgtggtca
    34441 aagaaatgat ggctaacttt tctcagacag gatatgaaaa caaacgacaa gtttttatta
    34501 tcaaagattg tgacaaaatg cataccaatg ccgctaatag cttgctaaag tatattgagg
    34561 agcctcaggg agaagcttac atgtttttgc tgaccaatga tgataacaaa gtgcttccga
    34621 ccattaaaag tcggacacag gtttttcagt ttcctaaaaa cgaagcctat ctttaccaat
    34681 tggcacaaga aaagggatta ttaaaccatc aggctaagtt agtagccaaa cttgccacaa
    34741 acaccagtca tctagaacgt ctgttgcaaa cgagcaagct tttagaactg ataactcaag
    34801 cagagcgttt tgtatctatt tggctgaaag atcagttgca ggcatattta gcgttgaatc
    34861 gtctggtaca gttagcaact gaaaaagaag aacaagattt agttttgacc cttttgacct
    34921 tgctcttggc aagagagcgt gcgcaaacgc ctttgacaca attggaggct gtttatcagg
    34981 ctaggctcat gtggcagagc aatgttaatt ttcaaaacac attagaatat atggtgatgt
    35041 cagaataagt gcaattggaa ttcgttatgg ggaacgaagc ttgtcaaaag acttgccctt
    35101 agcgaccaaa gtagcggcgc ataggggcaa aacaagcttt tgaagaaaat actgcctttg
    35161 caacagattt gatgaaagaa gtatcacgat taataacaca aaatcaactt gaaatgacgt
    35221 ttattaatag tgtctttccc ttagatcagc atgttgtcct aattcccttt tcagcagaag
    35281 agcacatgga tttttacaac tgttaaaaga tttagcggcc gattttaggt caagaagtga
    35341 gctgccccag attaacagcc aagaagaaac caagatttat gggcgcttag gaccgtgtgg
    35401 cagaccactg tgctgctttc gtttttggga aaatttctga cagtatctat taaaatacta
    35461 aaaaatcaaa ggttatcatt gactttgggt aaagcaacaa gcccttatgg ccgtttgctt
    35521 tgctgtctct atcgatgaag gtggctttta ccaataaagc aagaaaaaat tccctgaata
    35581 tggtaccata gtagagacaa gtgatggccc tgcaacagta gcagccttga tatatttaca
    35641 gatcccatta aggtgcggtt aaaagaccag ttgaccttag tcacttatgc tttagaggag
    35701 gttaaagtag gtgaataaaa aagaattatt cgatgctttt gatggatttt cccaaaattt
    35761 aatggttact ttagctgaaa ttgaagccat gaaaaaacaa gtccaatcac tagtagaaga
    35821 aaacacgatt cttcgcctag aaaatacaaa actacgggaa cgtttgagtc acctagaaca
    35881 tgaaacggta gctaaaaatc cttccaaaca aaggaaggat cacttagagg gaatttatga
    35941 tgaagggttt catatctgca attttttcta tggacaacgt cgtgaaaatg atgaagaatg
    36001 tatgttttgc agagaattat tagatagaaa gtaagggcat gcaggttcaa aaaagtttta
    36061 aagacaaaaa aacaagcgga accttgtatt tggttccaac cccgattggc aatctgcagg
    36121 acatgacctt tagagcagtg gcaacgttaa aagaggttga ttttatttgt gcagaagaca
    36181 caagaaacac aggtcttttg ctaaaacact ttgacattgc taccaagcaa atcagctttc
    36241 atgagcacaa cgcctatgag aagattcctg atttaattga tttattaata tcaggacggt
    36301 cattagctca ggtgtctgat gcaggaatgc catcgatttc tgatcctggg catgatttgg
    36361 tcaaggctgc tattgatagt gatatcgcag tcgttgcttt accaggtgcc tcagctggga
    36421 ttacagcttt gattgctagt ggactggctc ctcaacccca tgttttttat gggtttttac
    36481 ccagaaaagc agggcaacaa aaggctttct ttgaggacaa acaccattac ccagagacac
    36541 aaatgtttta tgaatcaccc tatcgcatca aggataccct ggccaatatg ttgacttgct
    36601 acggggaccg tcaagtagtg cttgtcagag aattaaccaa gttatttgag gaataccaac
    36661 gggggtctat ttcagaaatc ttatcctacc ttgaggaaac accactcaaa ggggaatgtc
    36721 tgctaattgt gtctggatcc caagtagaca gtgaggttga gttgacagct gaagttgact
    36781 tagtttctct agtgcaaaaa gaaatccaag caggtgccaa accaaaccag gctatcaaaa
    36841 cagttgctaa agcttatcag gttaatcgcc aagagcttta ccaacaattt catgacttat
    36901 aagtcatcat ataataggag aaaaactaat gacagaagat caaacagcag ttcttatttt
    36961 agggacatac ggaatcatat tggctatatt tttattatca gccttactat gccttatcgc
    37021 gaactggcga ctctttaaaa gagcgggtta caaaggatgg tacagcctta ttcctttcta
    37081 tgcaggtttt attaaacaca aaatcacttt tggagaagat aataaatggt tttactttat
    37141 tggttgggtg attgctattt attattatta tgttcacttt tgttatgttc gtgcttttgg
    37201 tggatcaaag gggctagcag tatttggctt gtttttccca ggcattgcta ccttgattat
    37261 cgcttttgga aaagcttata ccgcagaaga ataccaccct gtgccacatc ttttaaacta
    37321 agagagaaaa gaccatcaat tggatttatt gatggtcttt taggtgttta gatgattttt
    37381 gtgccgtgaa caatggctgt tccagtgagt tgactcaagc tttggcaatt ctcagcagtg
    37441 acgcctcctc caatcatgat ttcaatgcgt ttattggcat acgtaacaag gtcttttagt
    37501 tgttccacat tgtctgtaat aggagtagct tcaggtgagc catgagttag tacacgcaca
    37561 aagccataat caatcagttg atcaagcact tggtgctggt ggtcagtcgg aatccggtca
    37621 aaggccatat gaaagaccaa aggaagtcct tgtgttgcag gtagtaactg ctcgatagca
    37681 tccgtatcga gttgattttc cgaagtcagc aggcctaaca caagcgcatc agagccagcc
    37741 tcaatggctt tgagaatgtc ttcttccata gcctttagtt cgaggtcgtt atagacaaaa
    37801 tcaccaccac ggggacgaat catggttgct acactgatgt ttttatcgtg taaaagctgg
    37861 caggcttctt tgatgacacc gtaagatggc gtggtccctc cgacagctag gttatcacag
    37921 agttccacac gggtaacttg accagcatca agggttggaa gtagcgttag gttctcagca
    37981 caaaattctt taatcatttt tagatctcct ttatcactaa tattatagca cgaatgaagc
    38041 tagtcatcag gaaacatttt tgtcaaatca tggacattat gctatactca ctagtattaa
    38101 aagtaggcca aaacagcagc aaatactttg aaaaaagatt gctatgttct acggcaatat
    38161 cagtctttac caacgaactc tcaagaagaa aagaaagaca aagcttagca ttttaaaaaa
    38221 gccccctttt tttgtgaaat gatgaccaaa aacgggcgcc tcacgcctaa aacagttacc
    38281 ctaaaggaga acaaatgatt acgatttacc aatacccaaa atgtagcacc tgccaaagag
    38341 ccatggctga attaaagcaa ctggttagtg actttgaagc cattgacatc aaagctaatc
    38401 cacccaaagc tcaggacctc aaacattgga tggaaacatc tggttatacc atcaaaaatt
    38461 tctttaatac cagtggtaac agctaccgcg aattgggact taaagataaa atagaccagc
    38521 ttagccttga taaagctgct gaactcttag ccacagatgg tatgctgatt aaacggccta
    38581 ttctgattaa ggacggtaaa gtcctacaag ttggctatcg caagccatat caagagttgg
    38641 atttaggata atgcgacaaa gtctcaaaaa aggacaaggc aacattataa cgccttgtcc
    38701 ttttttggga tctgttttta gacattagag atcaatatct agtaaaattg gcgtatggtc
    38761 ttgacgttcc ccagaagaaa tcatttcaga acgcttaacc ttatccacga ttcggtttga
    38821 agctagccaa tagtcaattc tccaaccggt attattgatt ttgctagttt tactacgttg
    38881 tgcccaccag gtataaacat gaggaatatc gccgtgcacg tgacgaaaag tatctgtaaa
    38941 gcctcttgct aataggttag taaagccttg acgttcttcg tcggtaaaac caggggaacg
    39001 tcgattgctg cttggatttg ccaaatcaat ctctttgtgg gcaacgttgt aatctcccgt
    39061 agccaaaaca ggcttttgag catccagttc agtcaagtaa tcagcgtatt tgtggtccca
    39121 aatttgacgg tcatctaaac gtctaaggcc atcaccagca ttaggcgtgt aaacctgtgt
    39181 gacgaaaaag tcttcaaatt ctagggtaat gatacggccc tcagcatcca ttgttgttgg
    39241 ggcaccaatt tctggaaaag taatgacagg gtttagcgtg tttttgtaca ggaacatggt
    39301 gccagcatag cctttcctag caggctcaac tgaagaacgc cacacatgca aatagtttgg
    39361 aaagtaacta agtagggtct caatatgttt tttggtaggc cccttagcag ataatttggt
    39421 ttcttgaatg gcaataatat ccgcatcttg agcgactaaa gtgtctaaaa cagcgcgtga
    39481 taaaagcgca cgaggggact ctcctgtgag agcagcattt aaggaatcaa tgttccatga
    39541 aatcagtttc atttggtttc tctttctaac agatagtctt gttgttattg taacaaaaaa
    39601 cgaaacgata agcgactttg ggcacctagc ccccataaaa cagtctagcc aaatgaagca
    39661 tagcagcgta agaacaagac aaggaggtga tcagtgagca tatcgtttga cagttaagag
    39721 taggattact ataatagaag cataaatgta agcgctaaca aaacaaaaaa tataggagaa
    39781 tgtcaatggc acaaaaaaca gtcatcaccg aagaaacaac cgattttgtt atggatttca
    39841 aaacaagtag cgctgaggga aatgttgatt ttattaacgt ttttgacctt gagaaaatgg
    39901 cacaacaggt catccctaaa ggagcctttg gttatattgc tagtggtgct ggtgatacct
    39961 ttaccctcca tgaaaatatt cgctcattta atcataaatt aattgttcct cactccctca
    40021 aaggggttga aaatccgtca acagagatta cgtttgatgg tgatcactta acctctcctc
    40081 ttattttagc acctgtagcg gctcataagc tggccaatga acaaggagaa gtagcgtctg
    40141 ccaaaggatt gaaggaattt ggaagtatct acaccactag ttcctactca acaactgacc
    40201 tgccagaaat cagtgctgcc ttaggtggga caccccattg gtttcaattt tattacagta
    40261 aagacgatgg cattaatcgt aatattatgg atcgtgtcaa agcacaaggt tgcaaagcta
    40321 tcgttttgac agcagatgca acagttggag gaaaccggga agttgatagg cgaaacggtt
    40381 ttgtttttcc ggtcggtatg ccaattgttc aagaatattt acctgatggt gcaggtaaaa
    40441 caatggatta tgtctataaa tcagctaaac aagcgcttac ctcaaaagat attgaatata
    40501 tcgcaactta ctcaggcctg cctgtttatg tcaaaggacc acagtgtgct gaggatacct
    40561 tgcgggcatt ggacgcagga gcatcaggta tctgggtaac caatcatggt ggccgtcaat
    40621 tagatggtgg tccagctgct tttgattcct tacaagaagt cgctgaagca gttgatcaaa
    40681 aagttcccat tgtctttgac tcaggtataa gacggggtca acatattttc aaagctcttg
    40741 cttcaggtgc tgaccttgtt gccttggggc gtccagcgat ttacggcttg gctatgggag
    40801 gaagcactgg tactcgtcag gtctttgaaa agttaaatga cgagttaaaa atggtcatgc
    40861 aactggctgg gacacagact atccaagatg ttaaagcctt taaccttcgc cacaatccgt
    40921 atgattcatc gattccattt gatcaaaatg cgctacgctt agattagaac agacaaaaac
    40981 ctctgctatc atgtcacaaa tagtagaggt tttacactgc aaaaagaggt atgaaaattc
    41041 aataacatag ttacaaggct atcgtttacc acccccacta atagattaaa atgaaagact
    41101 tcttacatta atagaagttt ctgttgcttt tctcagaaaa atgtggttaa ataatcaaat
    41161 attattaacg aaaaatgagt taatgactaa cattaaactg gttttagtta aaacatttag
    41221 gagggtatca ggtggagaaa aagcaacgtt tttcccttag aaaatacaaa tcaggaacgt
    41281 tttcggtctt aataggaagc gttttcttgg tgatgacaac aacagtagca gcagatgagc
    41341 taagcacaat gagtgaacca acaatcacga atcacgctca acaacaagcg caacatctca
    41401 ccaatacaga gttgagctca gctgaatcaa aaactcaaga cacatcacaa atcactccca
    41461 agacaaatcg tgaaaaagag caaccacaag gtctagtctc tgagccaacc acaactgagc
    41521 tagctgacac agatgcagca tcaatggctg atacaggtcc tgatgcgact caaaaaagcg
    41581 cttctttacc gccagtcaat acagatgttc acgattgggt aaaaaccaaa ggagcttggg
    41641 acaagggata caaagggcaa ggcaaggttg tcgcagttat tgacacaggg atcgatccgg
    41701 cccatcaaag catgcgcatc agtgatgtat caactgctaa agtaaaatca aaagaagaca
    41761 tgctagcacg ccaaaaagcc gccggtatta attatgggag ttggataaat gataaagttg
    41821 tttttgcaca taattatgtg gaaaatagcg ataatatcaa agaaaatcaa ttcggggatt
    41881 ttgatgagga ctgggaaaac tttgagtttg atgcagagcc aaaagccatc aaaaaaaaca
    41941 agatctatcg tccccaatca acccaggcac cgaaagaaac tgttatcaaa acagaagaaa
    42001 cagatggctc acatgatatt gactggacac aaacagacga tgagaccaaa tacgagtcac
    42061 acggtatgca tgtgacaggt attgtagccg gtaatagcaa agaagccgct gctactggag
    42121 aacgcttttt aggaattgca ccagaggccc aagtcatgtt catgcgtgtt tttgccaacg
    42181 acgtcatggg atcagctgaa tcactcttta tcaaagctat cgaagatgcc gtggccttag
    42241 gagcagatgt gatcaacctg agtcttggaa ccgctaatgg ggcacagctt agtggcagca
    42301 agcctctaat ggaagcaatt gaaaaagcta aaaaagccgg tgtatcagtt gttgtagcag
    42361 caggaaatga gcgcgtctat ggatctgacc atgatgatcc attggcaaca aatccagact
    42421 atggtttggt cggttctccc tcaacaggtc gaacaccaac atcagtggca gctataaaca
    42481 gtaagtgggt gattcaacgt ctaatgacgg tcaaagaatt agaaaaccgt gccgatttaa
    42541 accatggtaa agccatctat tcagagtctg tcgactttaa aaacataaaa gatagcctag
    42601 gttatgataa atcgcatcaa tttgcttatg tcaaagagtc aactgatgcg ggttataaag
    42661 cacaagacgt taaagataaa attgctttaa ttgaacgtga tcccaataaa acctatgacg
    42721 aaatgattgc tttggctaag aaacatggag ccctgggagt acttattttt aataacaagc
    42781 ctggtcaatc aaaccgctca atgcgcctaa cagctaatgg gatggggata ccatctgctt
    42841 tcatatcgca cgaatttggt aaggccatgt cccaattaaa tggcaatggt acaggaagtt
    42901 tagagtttga cagtgtggtc tcaaaagcac cgagtcaaaa aggcaatgaa atgaatcatt
    42961 tttcaaattg gggcctaact tctgatggct atttaaaacc tgacattact gcaccaggtg
    43021 gcgatatcta ctctacctat aacgataacc actatggtag ccaaacagga acaagtatgg
    43081 cctctcctca gattgctggc gccagccttt tggtcaaaca atacctagaa aagactcagc
    43141 caaacttgcc aaaagaaaaa attgctgata tcgttaagaa cctattgatg agcaatgctc
    43201 aaattcatgt taatccagag acaaaaacga ccacctcacc gcgtcagcaa ggggcaggat
    43261 tacttaatat tgacggagct gtcactagcg gcctttatgt gacaggaaaa gacaactatg
    43321 gcagtatatc attaggcaac atcacagata cgatgacgtt tgatgtgact gttcacaacc
    43381 taagcaataa agccaaaaca ttacgttatg acacagaatt gctaacagat catgtagacc
    43441 cacaaaaggg ccgcttcact ttgacttctc gctccttaaa aacgtaccaa ggaggagaag
    43501 ttacagtccc agctaatgga aaagtgactg taagggttac catggatgtc tcacagttca
    43561 caaaagagct aacaaaacag atgccaaatg gttactatct agaaggtttt gtccgcttta
    43621 gagatagtca agatgaccaa ctaaatagag taaacattcc ttttgttggt tttaaaggac
    43681 aatttgaaaa cttagcagtt gcagaagagt ccatttacag gttaaaatct caaggcaaaa
    43741 ctggttttta ctttgatgaa tcaggtccaa aagacgatat ctatgtcggt aaacacttta
    43801 caggacttgt cactcttggt tcagagacca atgtgtcaac caaaacgatt tctgacaatg
    43861 gtctacacac acttggcacc tttaaaaatg cagatggcaa atttatctta gaaaaaaatg
    43921 cccaaggaaa ccctgtctta gccatttctc caaatggtga taacaaccaa gattttgcag
    43981 ccttcaaagg tgttttcttg agaaaatatc aaggcttaaa agcaagtgtc taccatgcta
    44041 gtgacaagga acacaaaaat ccactgtggg tcagcccaga aagctttaaa ggagataaaa
    44101 actttaatag tgacattaga tttgcaaaat caacgaccct gttaggcaca gcgttttctg
    44161 gaaaatcgtt aacaggagct gaattaccag atgggcatta tcattatgta gtgtcttatt
    44221 acccagatgt ggttggtgcc aaacgtcaag aaatgacatt tgacatgatt ttagaccgac
    44281 aaaaaccggt actatcacaa gcaacatttg atcctgaaac aaaccgattc aaaccagaac
    44341 ccctaaaaga ccgtggatta gctggtgttc gcaaagacag tgtcttttat ctagaaagaa
    44401 aagacaacaa gccttataca gttacgataa acgatagcta caaatatgtc tcagtagcag
    44461 acaataaaac atttgtggag cgacaagctg atggcagctt tatcttaccg cttgataaag
    44521 caaaattagg ggatttctat tacatggtcg aggattttgc agggaacgtg gccatcgcta
    44581 agttaggaga tcacttacca caaacattag gtaaaacacc aattaaactt aagcttacag
    44641 acggtaatta tcagaccaaa gaaacgctta aagataatct tgaaatgaca cagtctgaca
    44701 caggtctagt cacaaatcaa gcccagctag cagtggtgca ccgcaatcag ccgcaaagcc
    44761 agctaacaaa gatgaatcag gatttcttta tctcaccaaa cgaagatggg aataaagact
    44821 tcgtggcctt taaaggcttg aaaaataacg tgtataatga cttaacggtt aacgtatacg
    44881 ctaaagatga ccaccaaaaa caaaccccta tctggtctag tcaagcaggc gctagtgcat
    44941 ccgctattga aagtacagcc tggtatggca taacagcccg aggaagcaag gtgatgccag
    45001 gtgattatca gtatgttgtg acctatcgtg acgaacatgg taaagaacat caaaagcagt
    45061 acaccatatc tgtgaatgac aaaaaaccaa tgatcactca gggacgtttt gataccatta
    45121 atggcgttga ccactttact cctgacaaga caaaagccct tggctcatca ggcattgtcc
    45181 gcgaagaagt cttttacttg gccaagaaaa atggccgtaa atttgatgtg acagaaggta
    45241 aagatggtat cacagttagt gacaataagg tgtatatccc taaaaatcca gatggttctt
    45301 acaccatttc aaaaagagat ggtgtcacac tgtcagatta ttactacctt gtcgaagata
    45361 gagctggtaa tgtgtctttt gctaccttgc gtgacctaaa agcggtcgga aaagacaaag
    45421 cagtagtcaa ctttggatta gacttaccgg tccctgaaga caaacaaata gtgaacttta
    45481 cctaccttgt gcgggatgca gatggtaaac cgattgaaaa cctagagtat tataataact
    45541 caggtaacag tcttatcttg ccatatggca aatacacggt cgaattgttg acctatgaca
    45601 ccaatgcagc caaactagag tcagataaaa tcgtttcctt taccttatca gctgataata
    45661 acttccaaca agttaccttt aagatgacga tgttagcaac ttctcaaata actgcccact
    45721 ttgatcatct tttgccagaa ggcagtcgcg ttagccttaa aacagctcaa ggtcagctaa
    45781 tcccgcttga acagtccttg tatgtgccta aagcctatgg caaaaccgtt caagaaggca
    45841 cttacgaagt tgttgtcagc ctacctaaag gctaccgtat cgaaggcaac acaaaggtga
    45901 ataccctacc aaatgaagtg cacgaactat cattacgcct tgtcaaagta ggagatgcct
    45961 cagattcaac tggtgatcat aaggttatgt caaaaaataa ttcacaggct ttgacagcct
    46021 ctgccacacc aaccaagaca acgacctcag caacagcaaa agccctacca tcagcgggtg
    46081 aaaaaatggg tctcaagttg cgcatagtag gtcttgtgtt actcggactt acttgcgtct
    46141 ttagccgaaa aaaatcaacc aaagattgat aacaaaacgc aaagagacaa caaatcatcc
    46201 cttttaacag tcggaccttt ctttgaaatt gagctggcag atgatcattt cttaacaaaa
    46261 gttaaagagt tgagcaaagt aaggaacaga cataaagacc ataaaagcaa gccagtttgg
    46321 cttgctttta tggtgttatt gtcgtgtgct acgatgacta aaaaaggaca acgaacaatg
    46381 acctcgaatc accaaaagga cttgatttga tagcttccct atgctatact aagcctattc
    46441 tttcaagaag gactcctttt gtggaaaaga ctgaaaaaaa ggacactaat ctatccaaaa
    46501 actccagata ggtagctgca aacgatgcct ctgctgtgat caaggggata aagcagactg
    46561 gcttgtcact acctgagcag gtgtcaaaaa aagaagcttt tggctttgtc attcaacata
    46621 ctgagcttta gtctttcaga acggagacac ccccagtttt gtactcaaaa agcataaaca
    46681 gctctgctga tgtgacatcc gatttctacg tgcgcagtgg gtcaaaacag ctgctgtttg
    46741 ctatgataaa agtggcgaca ctactaacat tgacgccaat ggggattttt gagactctag
    46801 gctcaaaaat aaggcataga attccaaagg aagtcgctgc cgtgagcaca acctaagaaa
    46861 aactatccca aaagactttt tctgcaattt gaaatactga atttcagtat tttttctttt
    46921 gtaactaaga gcaatgaatg aggtaaaaaa ggcaacaatg tcaaaaaaga aacgaaaaca
    46981 ctatcaggtt tatgagggat tacgctgtgc tatgacgctt tgttttatca gtggctatgt
    47041 caatgccttt acctatatga cccaaggcaa acgttttgca ggtgttcaga caggcaacct
    47101 tttgtcattt gccattcgct tgtcagaaca gcaactgaag gaggcactgc aatttttact
    47161 tcccatgata gtttttatgc ttgggcagtc ctttacttat ttcatgcacc gctgggccac
    47221 taaaaaaggg ctccactggt acctcttatc aagctttatc ttaacaggga ttgcttttgg
    47281 aacggcccta ttcacgcctt ttcttccttc caatgttacg gtagcagctt tagccttttt
    47341 tgcctctatt caggttgata cgtttaaaac cttacgagga gctagctatg ctaatgtgat
    47401 gatgacagga aatatcaaaa atgccgccta tttactaacc aaaggcttgt acgaaaaaaa
    47461 tcacgaacta acacatatcg gacgcaacac cttaatagtc atcttggctt ttgctgttgg
    47521 ggtggtttgt tcgacgttgc tttgcatagc ttatggcgag tatgctttga tgcctatttt
    47581 aatgccttta ctctatgtca attacctgtt ggctcaggaa ttttatcaca ttcaaaccaa
    47641 aatcaaaccg attaaaagaa cttaacggac ttgtggagaa agaaagttca tttagggtaa
    47701 gtttttactt accctccgtt gatttgctgg ttttgctgcc ccttaaaata tgatataata
    47761 gaccattgat agctgttact taacagaagg ctgataaaaa aggttccatt agccttgagg
    47821 gttgaattta aacaggaaaa aacgaaatcc tacactatgc atctcaatcg tactttcatt
    47881 tgagtatggt tctgaaagga aactatgatg aaaaaaccat tttacattac gacacccatt
    47941 tattacccat ctgggaaatt acacattggt tctgcctata caactattgc ttgtgatgtc
    48001 ttggcacgct acaagcgtct gatgggccat gaggtctttt acctcacagg actagatgaa
    48061 cacggacaaa agattcaaac caaggcaaaa gaagctggta ttacacctca aacctacgtt
    48121 gataacatgg caaaggatgt caaagccctt tggcaattgc tagatatttc ctatgacaca
    48181 tttatccgga caacggacga ttaccacgaa gaggtcgttg ctgctgtttt tgaaaaatta
    48241 ctggcgcaag atgacattta ccttggtgaa tactctggtt ggtattctgt ctctgatgag
    48301 gaatttttca ctgaaagcca actagaagaa gtttttagag atgaagacgg taaggttatt
    48361 ggagggattg ctccttcagg tcaccaagta gagtgggtat cagaagaatc ttacttccta
    48421 cgccttagca agtacgctga ccgcctcgta gccttcttta aagagcgacc tgactttatc
    48481 caaccagacg gtcgtatgaa tgaaatggtg aaaaacttta tcgagccagg cctagaagat
    48541 ttggctgtta gccgtactac ctttacatgg ggtgtacctg ttccatcaga cccaaaacac
    48601 gtggtttatg tctggatcga tgccctccta aactacgcga cagctcttgg ttatggtcaa
    48661 gctaatcacg ctaactttga taaattctgg aatggaacgg ttttccacat ggttggaaaa
    48721 gatatcttgc gttttcatag catctactgg ccgatcttac tcatgatgct tgatttacca
    48781 atgccagacc gcttaattgc ccatggttgg tttgtcatga aagacggtaa aatgtctaaa
    48841 tcaaaaggca atgtggtcta tccagaaatg ttagtggagc gctttggact tgatcctctt
    48901 cgttattacc tcatgcgttc tttgccagtt ggttcagacg ggacctttac ccctgaagat
    48961 tatgtgggtc gtatcaacta tgagttagcc aacgaccttg gcaatttatt gaaccgtacc
    49021 gttgccatga tcaacaagta ttttgacggc acggtgccag cttatgttga caatggaact
    49081 gcctttgatg ctgacttcag tcaactgatt gatgctcagc tagcagacta ccataagcac
    49141 atggaagctg tagactaccc acgcgcctta gaagccattt ggaccgtcat tgctcgtacc
    49201 aacaaataca tcgatgaaac cgctccttgg gtgctcgcta aagaagatgg tgacaaagct
    49261 caactagcca gtgtcatggt acacttggca gctagcttgc gcgtggtcgc tcatgtgatc
    49321 caacccttta tgatggaaac atcagctgct atcatggccc agcttggctt agcgcctgtt
    49381 tctgacttat caacacttgc tctagcagat tttcctgcca atacaaaggt cgttgccaag
    49441 ggcacaccga ttttcccacg tcttgacatg gaagctgaga ttgactacat taaagcccaa
    49501 atgggtgaca gctcagctat ttcccaagaa aaagaatggg tccctgaaga ggttgcgctc
    49561 aaatctgaaa aagacgtcat cacatttgaa acctttgatg ctgtggaaat ccgtgtggca
    49621 gaagtcaagg aagtgtcaaa agtggaaggt tcagaaaaat tgctgcgctt tagagtagat
    49681 gcaggagacg ggcaagaccg tcaaatcttg tcaggtattg ccaaatttta ccccaacgaa
    49741 caagaattag tgggtaaaaa actacaaatt gttgctaacc ttaaaccgcg taaaatgatg
    49801 aaacaataca tcagtcaagg catgattcta tcagcagagc atggagatca actaacggtc
    49861 ttaaccgttg attcctctgt tccaaatgga agtatcattg gctgatgctg acctattatt
    49921 caaaaaacct aagcaagaat atgtgcttag gtttttttga ttgccttcaa gaaagacgaa
    49981 taacgagatg ggtgatatgg catcaaagca taactatcct aggacctgtc ctagtaagat
    50041 tgctgtgctt ttgacaagtg aacagagagg tcttaaacta tatcttgtca taaaaaagat
    50101 tgcaaaacac tatatcttgt gttattatgg tttttataaa agttaagcat gcttaacttt
    50161 agccaaacaa aagaggacac tagagcgtgt cccacaagac agttgagccc ttcgcttttt
    50221 tagtagagta ggagtgctca agtcaaaaag agacatccca tcaaaccatt gctgtttagc
    50281 aggtcttttt gtgttgctca aaaaacagtt cctgtctgtt tggcgtctag tgatgactta
    50341 ggcttagcgc tactagactt ttgtttgaaa aatacctcaa gccttgaagg agacaaatga
    50401 cccaacatta ttatgaacgt tcgcaatccc caattgaata cgccctatct gaaacacaaa
    50461 aacagttgcg ctccatcaat tggaattacc ttaatgatga caaagatttg gaagtgtgga
    50521 accgtgtcac tcaaaatttt tggttgcctg aaaaggttcc tgtttctaat gatttaaatt
    50581 cttggcgctc cttgggtgag gattggcaac agctcatcac aaggacctac actgggttga
    50641 ccttactaga tacagtacaa gcaacggttg gtgatgtggc acaaattcag cattcacaaa
    50701 ccgaccacga acaagtcatc tatacgaact ttgcctttat ggtgggaatc catgcccgct
    50761 cttacgggac gattttttcg accctttgca gcagcgagca aatcgaagaa gcacatgaat
    50821 gggtggtctc aacacagagc ttgcaagacc gtgcccgtgt cttgattcct tactatacgg
    50881 gtgatgaccc tttgaaatca aaagtagcag cagctatgat gccaggattt ttgctgtatg
    50941 gcggctttta ccttcctttt tacctatcag cacgtggtaa aatgccaaac acatctgata
    51001 tcatccgcct gattttacgc gacaaagtta ttcacaacta ttacagcggt tacaaatacc
    51061 aacaaaaagt agcacgtctt agcccagaaa aacaggctga aatgaaggcc tttgtctttg
    51121 acttgctcta tgaactcatc gaccttgaaa aagcttactt gagagaactg tatgcaggct
    51181 ttgatttggc agaagatgct atccgcttta gcctttataa cgctggaaaa ttccttcaaa
    51241 atttgggata cgagtcgccc ttcacagatg aagaaacgcg cgtttcacca gaagtttttg
    51301 ctcagctatc agcgcgtgca gacgaaaacc atgacttttt ctcaggcaat ggctcatctt
    51361 atgtgatggg gatcaccgaa gaaaccacag atgacgattg ggagttttaa gaaatggcag
    51421 agctgattat tgtgtatttt tcatctaagt ccaataacac ccatcgcttc gtccaaaaat
    51481 taggcctgcc tgctcagcgc ataccagtag ataataggcc acttgaagtt tcaacccatt
    51541 accttttgat tgtgcctacc tatgcagctg gtggctcaga tgccaaagga gccgttccta
    51601 aacaagtgat tcgttttttg aataatccta acaatcgtaa acattgtaag ggagtgatct
    51661 cttcaggaaa taccaatttt ggcgatacct ttgccatagc aggccctatc atttctcaaa
    51721 aattgcaggt ccccttatta caccaatttg aactattagg cactgctaca gatgtcaaaa
    51781 aggtccaggc gatttttgcc cgcttaaagc atcacacaca cgacaagcaa aaacaaacca
    51841 acaacctgat tacagaaagg acacacccat gtcacaaacc aatgcgtcat acctctcatt
    51901 aaatgcactg acccgtttta aaaaaccaga cggcagctac cattttgata gcgataaaga
    51961 agctgttagg cgctacctgg aagaacatgt ctctcccaat caaatggctt ttaactcact
    52021 agaagataag ctagcttacc tcatcaatga agggtattat gagcaggcca tttttgatgc
    52081 ctatcctaac gaccttatca aggaggcctt tcattatgcc tatcaacaag gctatcgctt
    52141 tttaaatctc atgggcgcta tgaaatttta ccagtcctat gccttaaaaa ccttggacgg
    52201 taagcagtat ctagaaacat ttgaagaccg tgctgtgatg aatgcactct ttttagctga
    52261 tggagatcaa acctttgtct ttgacgtgat tgatgccatc ttgcaccgac gctttcaacc
    52321 agctacccca acgtttttaa atgccggaaa aaaacgccgt ggcgaataca tttcgtgcta
    52381 tcttcttcgt gtcgaagaca atatggaatc tatctctcgc gccattagca ccagcttgca
    52441 actttcaaaa agaggaggcg gagtcgcctt gtgcctcacc aacctcagag aaatcggagc
    52501 accgataaaa gggattgaaa atcaggcaac aggcatcgtt cctgtcatga aactactaga
    52561 ggactctttt tcctatgcca atcaactagg tcagcgccaa ggagcaggtg cggtttacct
    52621 tcatgcccat catcctgagg tcctgacctt tttagacacc aaacgtgaaa atgccgatga
    52681 gaaaattcgc attaagtccc tagcattggg acttgtgatc ccagatatta ccttccagtt
    52741 agccaaagaa aacaaagaca tggctctctt ttcaccctat gatatcaagc gtgcctatgg
    52801 caaggatatg tcagacatct ccatcacaga ggaatacgat aaactgctag ccaatccagc
    52861 catcaaaaag acctacatca gtgctagaaa gttctttcag ttaattgcag agctacattt
    52921 tgagtcaggc tacccttacc tcttgtttga tgacacggtc aataagcgca atcctcatgc
    52981 caaaaaaggc agaatcgtca tgtctaacct gtgttctgaa atcgctcaag tcagcacccc
    53041 aagcactttc aaagaagatt tgagttttga aaccattgga gaggatatct gctgtaattt
    53101 agggtctatc aatattgccc aggctatggc agatgcgcca cactttgaac agctcatcac
    53161 aacgagcatc agagcacttg atcgtgtctc tagagtgtct gacctgaatt gtgcgccttc
    53221 tgttgagaca ggcaatgcag ccaaccatgc agtaggatta ggcgccatga acctacatgg
    53281 gtttttggca accaaccaca tctactatga tacaaaagaa gccgttgact ttacagatct
    53341 gtttttccat gccatggcct attatgcttt taaagcctct tgccaactag ccaaagaaaa
    53401 aggcgctttt gcaggatttt cgttatcaac ctatgcagat ggcacttatt ttgccaagta
    53461 tttgcaagaa gatgccaagc cacaaacagc caaggttgct acattgttgc aagattacgg
    53521 cttcacattg ccaacggtgg cggattggca agccctagta gcagatatca agcagtttgg
    53581 cctagccaat gcacacttgc tagcggttgc tccgacaggc tccatcagct acttatcatc
    53641 atgcactccg agtctacaac cagtcgttgc tcctgttgag gtccgaaaag aaggtagcct
    53701 gggcaggatt tacgtccctg cctatcagat tgaccaggct aactatgctt actatgaacg
    53761 tggtgcctat gaggtaggcc caaaagccat catcgacgtt gtcgctgctg cccaaaaaca
    53821 cgttgaccaa gccatctcat tgaccctgtt tatgaccgat caagcgacca cacgtgacct
    53881 gaaccgctcc tacatccaag cttttaagca aaactgtgca tcgatctatt atgtgcgagt
    53941 gaggcaagac gtcctagcag gcagcgaaca gtatgacgag gatagtcttg tgacagctcc
    54001 tggagctagt gatgagacca ccacagagtg tcaaagctgt atgatctaac agctactaga
    54061 aagctctcgt gtcacctatc acatgcatag gatcagtgcg ctcgaccttt caagacaagc
    54121 aagcatcagc tcttgcttgt ctttttttgg tttcaaagcc cgttagtttg ctgctatgcg
    54181 aggctttttt gagcatcaga acgtcaaaaa aaggacatgg agtccttttt tggtgatcgg
    54241 tgttgaggcc gtcacaaact gcccttgaag cacgcttcta tgtggagctt tttgtggtcc
    54301 tgtgacacgt aagctccaac gacctccagc tgacagcctc ttggaaacaa gagctcaacc
    54361 tctgaaggca cagccgaata aggctcgaca aaggctgctt tggccccttt tttgacacag
    54421 atgcgcactt ccaccggtcg atgggtcatg gcgccgtttt tcaaggctgt cgtgctcata
    54481 aaactgtgct tggtgtagcg tgtaccaagc ttgatcttac aaaggatatg agggtcaaac
    54541 tgatggttac ggtagtaaga cgtgatatca gcgtgtgaaa caccgatatc acgcaaaaac
    54601 gtctcgtata catagcggta taccacaatg ttccacggga tgactagccg gtgagtcgca
    54661 gcatcgagct gggccacctg atcccttagc tcaggcgtga gttggctgag ctcacccttg
    54721 gtcttgtcta gactggtatt gataagggct gcctgctgac aggtataagc agccatcgcc
    54781 tcttgctcag tccttgggta gacgagaccc ttgccccacc gctttttaat cagtgtctca
    54841 gctccctttt gatccgaaaa ggtaaaataa gcaggcgtcc cctcacaaac cgcccgctca
    54901 tagtactcac agacaaaaag ctcgaccaag ccctcagttt gccagatcag actaaaacag
    54961 ctaagaagaa ggatcatagc cagttgatag cgctttttta gcatagttcc ccctgacacc
    55021 agcccaaaag gctgaccctt cccctaggcc ttttggctag ctcttgatac caccagtttc
    55081 tcagacaggc taatcttttt gtcatctctt tctccctaaa cagactctct tatcatgata
    55141 ttatgctatt atgagcgatt tgtcaatcca tagcaatcgt tttttagtta gataagcaag
    55201 cttgtgacct agaggcagac aagagtggca gtacctaccg ttcgctgtgg tggctaataa
    55261 ggactaacat aaaatctcaa acgctttcaa aaacaactcc taagcttaaa aaaggctgat
    55321 gaacagattt gttgagctta agggagattg ccatgatttt ctaatcattt gatgtgtaaa
    55381 atgataaaaa aatgaacttg aaatgaaaga atgcgagtag taaactaaac ttattcaaaa
    55441 aataatgaaa tggagtgatt ttatgaaaac aagatcaaaa cgctttttaa acctagcaac
    55501 cctttgcttg gccctactag gaataacttt gctaatggca cgtcccgtaa aggcggagtt
    55561 cgtacctaca catcaacaga gtggtgagag ttcaagatta atgaatgatc ctggtgaaga
    55621 tagtagcagt gaggaaatga aacgagctaa agatcggaaa tgtggttatg atgagggtta
    55681 tgagctcgga caagagtcag atcatcgtaa ggtagataga aagagtattg aggttcccta
    55741 ctatgtagaa gaccaagatg aatataagga tggctatgaa cagggattcg aagcaggatg
    55801 gtacgatagt catccaatcg cagcactact agacatggct tggtactact taacatatac
    55861 atttgatact ttgtttggtg gtgcaaaatc tcagtaaata gcgtgtttgt tgccctaatt
    55921 tagttattgt gaagtattga aaagcttgag gatactttcc ttagctcaag catttctcac
    55981 actaatctgc tataattggg ataatccctt tagtagtcag gccacctctg ataggggtgg
    56041 cttttttggg ttttagtgac ccgtgtgttg agaaaggagc atttatgcca gaaaagaaag
    56101 tgttgcccgt tctaatatga accgacagtt attccttatt aaatagacgc aaatatccac
    56161 gctttcactg tggtggctga taagaactaa cacaaaatct caaacgcttt caaaaacagc
    56221 tcctaagctt aaaaaaggct gatgaacaga tttgttgagc ttaagggagg ttgccataat
    56281 tttctaatca tttgatgatt agaatgataa aaacatgaac ttgaaatgaa agaacactag
    56341 tagtaagcta taaccgtatc aaaaataatg aaatggagtg attttatgaa aacaaaatca
    56401 aaacgctttt taaaactagc aactctttgc ttggcgcttt tgagcacaac cttactgatg
    56461 gcacaaccgg taaaggcaag ggataatcag tcacaacaac aggagcatgt cagtaataat
    56521 attgtagaag attcccaaaa aaatgatagt gactatgatc agggctatga agaaggctat
    56581 aaagaaggtc aaaaagaagg tgctcccgag aatccagaca aaaaagatga agataaatta
    56641 aaaaataagc ctcctaggta tggtgatggt tattactcag gctactatcg agggtgggat
    56701 gtaaccaatc gtcctttcca aacattatta aaagatattt atctttggtt atgggagact
    56761 ttctttaaat agtaaataat tgttagttat ttataaaaat atagtcataa aatgcgataa
    56821 aaactaatta ttatgataat tgtacttgag gaagatagta ccttgtatgc atatagaagc
    56881 gtattgcaaa gatgtgctat aattggaata atctttctga taatcaggcc acttctgatg
    56941 ggggtggctt ttttggatta cttacccgag tgttgaaaca ggagcattta tgccagaaaa
    57001 gaaagtgttg cccgtcctaa gtatgatggt gagccttgtg ttgctgcttg cttttgctac
    57061 ttaatagcta cctctacttg ttttgtagcg agtagtctag ccactgtagg tcttgtataa
    57121 aacaaaagaa agtggttgat agctgggctt gtagtagcag taatagtaac gagctgtgtc
    57181 taatgtgagc cgacagttat tccctataaa atagacgcaa atatccacgc tctcactgtg
    57241 gtggctaata aggactaaca taaaatctca aacgctttca aaaaaagctc ctaagcttaa
    57301 aaaaggttga tgaacagatt tgttgagctt aagggagatt gccatttttt atcatttgat
    57361 gtttaaaatg ataaaaaaat gaacttgaaa tgaaagaatg cgagtagtaa gctataacta
    57421 catcaaaaat aatgaaatgg agtgatttta tgaaaacaaa atcaaaacgc tttttaaacc
    57481 tagcaaccct ttgcttggcc ctactaggaa caactttgct aatagcacaa cccgtgaaag
    57541 cagaggtgac agtagcaaca acagaaggag tgcaccaaac tgcggaagcg gaaaatgaag
    57601 atattattaa agaagagtat atgactctcc tcgggttaag cgatagcgat ttacaagatc
    57661 ctcttgaaga agctagatat aaaggatatg tagatggata tagaaaaggc tttggaggag
    57721 aggatagaat cgaccggagc gctattaagg atctttatta taatgttccg tcatctgaag
    57781 atggcgagta tagagatggt tatgagcaag gatttgaaaa aggaagacac gcaaattatc
    57841 cagtacgggc attcctagaa gatctactag gaggtttatg gggattcttg acaggtattt
    57901 ttggagacgg gtctagcgac actcagtaaa tgacacattt gttgtcttaa tgtagttatt
    57961 gtgaagtatt gaaaagcttg aggatacttt gcttagttca agccattccc acactaatct
    58021 gctataattg ggatagtccc tttagtagtc aggccacctc tatcagaggt ggcctttttg
    58081 ggttttagtg acccgtgtgt tgagaaagga gcatttatgc cataaaagaa agtgttgccc
    58141 atcctaagca tggtggtgag ttttgtctgc ctgcttgatc atatcttctt tttggggctc
    58201 tttgtcaact gtagtgggtg acttatagct aaacacgtga ggagacgaga ctcgtcttct
    58261 cttttgttat gttcaaagcg aggtaaatgc gttttttaaa gttatcaaag ttccgaaagc
    58321 caaaagcatt gcgtttgatg tgtttgatga gtttattggt tgcttctagt ttggcatttg
    58381 aacaaggaag ctcaatggca ttagtgacat agtttttgta tcgcatcagg gtgttcaaag
    58441 tcgttttaaa ggtgtgattg agcagaagta acaccttttg aattaagtca aaaaatgctc
    58501 agtattcttc tcttgtaagt gaaaaaggag taactgataa aggttatagt aatcttttaa
    58561 ttcaggaacg agattaaata gtttattgag acattgttta ggagttagcg tttccctaaa
    58621 agttctagag tagaaggcgt tgagcgagag tttacggcta ccttttgaat gagttgacag
    58681 tagtatttaa gaactctgta ttcaagagac tttttgtcat attgcttcat gatgtgaatc
    58741 cgggtttgac tgagcgctct gttcatgtgc tgaacaatat ggaaacgatc taggacgatg
    58801 ttagcttgag gaaatagctt tttaatgagc gaaatatagc ttcctgacat atcaacggta
    58861 acgattttga cactgtttct agcctcttta gagtgtctaa agaagtgatt tcgaatggtt
    58921 gtttgtcgcc ggttgtccag tatggtgatg atttttttgg actcaaaatc ttgagcgata
    58981 aaggcgagtt cgcccttttg ataggaaaat ccatcccaag acaacacctc gggtagttta
    59041 gagaagtctt ccttgattac aaactggttg agttttcggt agacggtaga agatgatata
    59101 gccgactgtt gagcgatgtt agtgagagcg cctcgattaa ccaaggtttg tgctactttt
    59161 tgtctgaggt gctcagagat ctgacagttt ttttgaacca gacttgtttc cgctacagcc
    59221 acttttcgtc aggacttgca ttgagatctt ctctttttta aacgaatgag actagggaaa
    59281 ccaccaattt cgataaaggg gattttagaa tgattctggg agtcgtattt aatctgaact
    59341 cctttacagt gtttacactt aggtggggga tagtcaagtg tggcgaagac ttcgataggt
    59401 gtctcgcgtt gaaaaacctt gttcaaggtg atgtgtttat ctttaattcc aatcaattct
    59461 gtagtatgat agagttgtct catatgattc tttctaatga tggtttggtc gctttgcatt
    59521 ataagtcata tgggattttt tgtgtacatc caaaaagtct tataatatct gtaggagagt
    59581 tacccactac agatattata gagccctttt ttgctactta tagttatctc tacttgccgt
    59641 ttgtagcaag tagtttggcc attgtaggta aaagcaagtg ttcgctggct gggcttgtag
    59701 tagcagtaac agtacaatct gaaccgacag ctattcccta ttaaaataga cgcaaatgtc
    59761 cacgctttca ctgtagttgc tagcaaggtc taacacaaaa catcaaaagc tttcaaaaac
    59821 aactcctaag cgcaaaaagg cggactaaca gccccaagca agcactttct gaggtaataa
    59881 gagattgcca tgattttcta atcatttgat gtttaaaatg ataaaaacat gaacttgaaa
    59941 tgaaagaatg cgagtagtaa actaaaaata tatcaaaaat aatgaaatgg agtgatttta
    60001 tgaaaacaaa atcaaaacgc tttttaaacc tagtaaccct ttgcttggcg ctactaggaa
    60061 caactttgct aatgggacgt cccgtaaagg cggaggaaga tagcgatatt aaaaaagaat
    60121 atatgaaacg tttgatgtta acagattcag atttagaacc cggggggtat tttagtaaga
    60181 gagttcaagg ttatttaaaa ggttatgcag atggtcagag aaaggatgct agagagccag
    60241 acagtaatag ttacggtcct aattttgacg cctatgatgg ttataaggat ggatatgatg
    60301 aaggttatgg tgaaggctgg cgtaaagcta atgaagctgg tggttcacac agcgacgagt
    60361 ctcagagcag tggtgggaca caagatcaag ataaagtaga aaaatctact gaaggatcac
    60421 acaatgaaga gtctcaggat aaccatgaag cttcaaaaga aggggagaat tctactgatg
    60481 atttaacagt ttctggtttg ttatctggga ttgttgaaac ttttgtagcg tttctgtggt
    60541 ctttgtttaa ctaaaaataa cttattggtt tcatattgct tttagttaag aatataaaaa
    60601 atgaaaaaaa cataaacttt aaatgaaagc gtgagagatg taaaacaaac acatcaaaaa
    60661 taaagaaacg gagtaatttt taggagaaca aaatcaaaac actttttaaa cctagcaacc
    60721 ctttgcttgg cccttttgag tacaacctta ctagtgacac agccagtaaa ggcggacggg
    60781 gggagtaggg acctcttaca attagcgttc aggaaagtag ttctagcaca atatcgacag
    60841 atagtaatcg acaatctttg aatagggagt cagaacaaga tgatgaggct gaagctcaag
    60901 atgctggatg gcaaaaaggt ttggaagcag gtaaaaattc atcaatatgg aaaccagcgt
    60961 tagaatttga tgaggttcct caacctgatg ttgaatatag tgattatgtc ttacaacagt
    61021 ataaggaagg ctatatggct ggttatgcat acggttgggg tgaaactcac gaattaggtt
    61081 tagccgtact atctgtctta caatgggtat ggggaagtat ttttggatat taatccatgt
    61141 tagagaatat ataattatgt tgttttgggt tgctttatca caagctattc ccacactaat
    61201 ctgctataat tgggatagtc cctttagtag tcaggccacc tctgataggg gtggcttttt
    61261 tggggtttag tgacccgtgt gttgagaaag aagcatgtat gccagaaaag aaagtgttgc
    61321 ccgtcctaag catggtggtg agttttgtct gcctgcttga tcatatcttc ttttttgcta
    61381 cttatagtta tctctacttg ccgtttgtag caagtagtct agctcttatc ggtctggtgc
    61441 aaaacaaagg caggcaaaag gtttggtcgc tagctgggct cgtcctagct gctctagttg
    61501 tggcagtagt ggcctatgcc caacggttat tccttatcaa atgcaaagtc cctccctctt
    61561 atataaacta tcaaaaacag ttcctaaagc tcaaaaaggt tatctaactg ctccaagcag
    61621 gcactttctg tgataaggat agcttgtcat tttttcctaa tcatttgata tttaaaatga
    61681 taaaaaaatg aacttgaaat gaaataatgc gagtagtaaa ctataaccgt atcaaaat
//
LOCUS       DAUCWU010000005        52374 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUCWU010000005 DAUCWU010000000
VERSION     DAUCWU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52374
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.005"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1451
                     /locus_tag="VGY24_000281"
     CDS             <1..1451
                     /locus_tag="VGY24_000281"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ4283298.1"
                     /translation="LLFAVCDNLDQHFNTFTDAQKIFENAYQDEGLKDDLISVVTGDL
                     GYFIEPTLTFEKLIQDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGST
                     PQKQNQTISNVMKTLNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSH
                     LMTQIVFLGREDQKGMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLAR
                     MNMMLHGVAIENQHLSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATTGFLTDPRF
                     SSYGVLAPKSKADFAFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAI
                     DTIIGLPSNIFYNTSIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDNHIKKI
                     LDAYKSRDNSDKFSYLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQ
                     EIAKTNAKLDQLMKQLVGTTKEAQDELDTFRN"
     gene            complement(2004..2609)
                     /locus_tag="VGY24_000282"
     CDS             complement(2004..2609)
                     /locus_tag="VGY24_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4283299.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(2590..3189)
                     /locus_tag="VGY24_000283"
                     /pseudo
     CDS             complement(2590..3189)
                     /locus_tag="VGY24_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3191..3526)
                     /locus_tag="VGY24_000284"
                     /pseudo
     CDS             complement(3191..3526)
                     /locus_tag="VGY24_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4072..5478)
                     /gene="lacG"
                     /locus_tag="VGY24_000285"
     CDS             complement(4072..5478)
                     /gene="lacG"
                     /locus_tag="VGY24_000285"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ4283300.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDGGYSGEIGVVHAL
                     PTKYPFDANNLDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFTALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGVNVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(5551..7248)
                     /locus_tag="VGY24_000286"
     CDS             complement(5551..7248)
                     /locus_tag="VGY24_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ4283301.1"
                     /translation="MNGLIAQIEKGKPFFEKMSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIESLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKEGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYMIELIVHSTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKIYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            complement(7248..7565)
                     /locus_tag="VGY24_000287"
     CDS             complement(7248..7565)
                     /locus_tag="VGY24_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4283302.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(7589..8572)
                     /gene="lacD"
                     /locus_tag="VGY24_000288"
     CDS             complement(7589..8572)
                     /gene="lacD"
                     /locus_tag="VGY24_000288"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ4283303.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(8576..9505)
                     /locus_tag="VGY24_000289"
     CDS             complement(8576..9505)
                     /locus_tag="VGY24_000289"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ4283304.1"
                     /translation="MILTVTLNPAIDVSYPLDELKFDTVNRVVDVTKTPGGKGLNVSR
                     VLNEFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPMLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFKALKAVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIEIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(9553..10068)
                     /gene="lacB"
                     /locus_tag="VGY24_000290"
     CDS             complement(9553..10068)
                     /gene="lacB"
                     /locus_tag="VGY24_000290"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ4283305.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGKAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(10103..10531)
                     /gene="lacA"
                     /locus_tag="VGY24_000291"
     CDS             complement(10103..10531)
                     /gene="lacA"
                     /locus_tag="VGY24_000291"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ4283306.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKSFVEGKYDGGRHQVRVDMLNKMC"
     gene            10977..11750
                     /locus_tag="VGY24_000292"
     CDS             10977..11750
                     /locus_tag="VGY24_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4283307.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            12411..12698
                     /locus_tag="VGY24_000293"
     CDS             12411..12698
                     /locus_tag="VGY24_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ4283308.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            12688..13023
                     /locus_tag="VGY24_000294"
     CDS             12688..13023
                     /locus_tag="VGY24_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ4283309.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            13175..13645
                     /locus_tag="VGY24_000295"
                     /pseudo
     CDS             13175..13645
                     /locus_tag="VGY24_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13978..14127
                     /locus_tag="VGY24_000296"
     CDS             13978..14127
                     /locus_tag="VGY24_000296"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283310.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQITLDTYSHATAVGEDKSIKQFAN
                     YLKNN"
     gene            complement(14247..14639)
                     /gene="rpsI"
                     /locus_tag="VGY24_000297"
     CDS             complement(14247..14639)
                     /gene="rpsI"
                     /locus_tag="VGY24_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ4283311.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(14660..15106)
                     /gene="rplM"
                     /locus_tag="VGY24_000298"
     CDS             complement(14660..15106)
                     /gene="rplM"
                     /locus_tag="VGY24_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ4283312.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(15324..15530)
                     /locus_tag="VGY24_000299"
     CDS             complement(15324..15530)
                     /locus_tag="VGY24_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4283313.1"
                     /translation="MKNLKLKAACAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(15527..16225)
                     /locus_tag="VGY24_000300"
     CDS             complement(15527..16225)
                     /locus_tag="VGY24_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283314.1"
                     /translation="MFDSKQNLAKSQWMFIIIIAVMDLVVLVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLNGISPFVDRRF
                     GKQGILMGMIATGMSLVTIGLAVREMMAGQLKMGSFFKAGGSGTLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(16361..17221)
                     /locus_tag="VGY24_000301"
     CDS             complement(16361..17221)
                     /locus_tag="VGY24_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4283315.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHAKNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(17318..17836)
                     /locus_tag="VGY24_000302"
     CDS             complement(17318..17836)
                     /locus_tag="VGY24_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ4283316.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     SQLKDFMSQLDR"
     gene            complement(17840..18586)
                     /gene="rlmB"
                     /locus_tag="VGY24_000303"
     CDS             complement(17840..18586)
                     /gene="rlmB"
                     /locus_tag="VGY24_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ4283317.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(18633..19430)
                     /locus_tag="VGY24_000304"
     CDS             complement(18633..19430)
                     /locus_tag="VGY24_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283318.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQISVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMVAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQANQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(19626..20039)
                     /locus_tag="VGY24_000305"
     CDS             complement(19626..20039)
                     /locus_tag="VGY24_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ4283319.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(20032..21375)
                     /locus_tag="VGY24_000306"
     CDS             complement(20032..21375)
                     /locus_tag="VGY24_000306"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ4283320.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYIGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKALHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITIVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(21403..21633)
                     /locus_tag="VGY24_000307"
     CDS             complement(21403..21633)
                     /locus_tag="VGY24_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283321.1"
                     /translation="MNKIRVGLYAIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(22112..22693)
                     /gene="cysE"
                     /locus_tag="VGY24_000308"
     CDS             complement(22112..22693)
                     /gene="cysE"
                     /locus_tag="VGY24_000308"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ4283322.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(22702..23454)
                     /locus_tag="VGY24_000309"
     CDS             complement(22702..23454)
                     /locus_tag="VGY24_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ4283323.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSLALVNAFHNFPVWAAKELYV
                     IEVEGVKVTPVFTDKEDMALFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYITVLNTLMSDDNVAADTMEKVYLVPAFVYPKDDNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(23447..25579)
                     /gene="pnp"
                     /locus_tag="VGY24_000310"
     CDS             complement(23447..25579)
                     /gene="pnp"
                     /locus_tag="VGY24_000310"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ4283324.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLVTIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGINYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(25859..26590)
                     /locus_tag="VGY24_000311"
     CDS             complement(25859..26590)
                     /locus_tag="VGY24_000311"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ4283325.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEIFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            complement(26600..27979)
                     /locus_tag="VGY24_000312"
     CDS             complement(26600..27979)
                     /locus_tag="VGY24_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ4283326.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     IGLLLNLTFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGYFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(28006..28290)
                     /locus_tag="VGY24_000313"
     CDS             complement(28006..28290)
                     /locus_tag="VGY24_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4283327.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(28283..30343)
                     /locus_tag="VGY24_000314"
     CDS             complement(28283..30343)
                     /locus_tag="VGY24_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ4283328.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPSDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHKLGLPDQKKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMDEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(30695..30964)
                     /gene="rpsO"
                     /locus_tag="VGY24_000315"
     CDS             complement(30695..30964)
                     /gene="rpsO"
                     /locus_tag="VGY24_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ4283329.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(31140..31340)
                     /locus_tag="VGY24_000316"
     CDS             complement(31140..31340)
                     /locus_tag="VGY24_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283330.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTAYWLF
                     GLASSVAYGGLVKAKRAHSTTR"
     gene            31466..31735
                     /locus_tag="VGY24_000317"
     CDS             31466..31735
                     /locus_tag="VGY24_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4283331.1"
                     /translation="MTQNVCQQNVTYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTYRIADLDALTQLRPLRYAAH"
     gene            31836..32450
                     /locus_tag="VGY24_000318"
     CDS             31836..32450
                     /locus_tag="VGY24_000318"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ4283332.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(32484..33026)
                     /locus_tag="VGY24_000319"
     CDS             complement(32484..33026)
                     /locus_tag="VGY24_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ4283333.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETRANLLSQAEALLAAI"
     gene            complement(33157..33585)
                     /locus_tag="VGY24_000320"
     CDS             complement(33157..33585)
                     /locus_tag="VGY24_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4283334.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(33695..38092)
                     /locus_tag="VGY24_000321"
     CDS             complement(33695..38092)
                     /locus_tag="VGY24_000321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ4283335.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVEKAVQDNLEAQKSLEAMMPPAEEATPAPKFDYKER
                     VAQRQAGFEKAAITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHERKDLM
                     PEGQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDYANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHNLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARLKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     VLGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            complement(38346..40202)
                     /locus_tag="VGY24_000322"
     CDS             complement(38346..40202)
                     /locus_tag="VGY24_000322"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ4283336.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSITSMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            complement(40400..41659)
                     /gene="rseP"
                     /locus_tag="VGY24_000323"
     CDS             complement(40400..41659)
                     /gene="rseP"
                     /locus_tag="VGY24_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ4283337.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(41732..42526)
                     /locus_tag="VGY24_000324"
     CDS             complement(41732..42526)
                     /locus_tag="VGY24_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ4283338.1"
                     /translation="MKERVVWGGLAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(42539..43288)
                     /locus_tag="VGY24_000325"
     CDS             complement(42539..43288)
                     /locus_tag="VGY24_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ4283339.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVHFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNHRQRRFGKV"
     gene            complement(43507..43872)
                     /gene="yajC"
                     /locus_tag="VGY24_000326"
     CDS             complement(43507..43872)
                     /gene="yajC"
                     /locus_tag="VGY24_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ4283340.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETIVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(43988..44362)
                     /locus_tag="VGY24_000327"
     CDS             complement(43988..44362)
                     /locus_tag="VGY24_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ4283341.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(44477..47974)
                     /locus_tag="VGY24_000328"
     CDS             complement(44477..47974)
                     /locus_tag="VGY24_000328"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ4283342.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKKKVS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWTKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(48172..49785)
                     /locus_tag="VGY24_000329"
     CDS             complement(48172..49785)
                     /locus_tag="VGY24_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ4283343.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAEELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLTGAQ
                     VIITNTDVDTVFETKHLQPWDAFCLKLKA"
     gene            complement(49909..51042)
                     /gene="ugpC"
                     /locus_tag="VGY24_000330"
     CDS             complement(49909..51042)
                     /gene="ugpC"
                     /locus_tag="VGY24_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ4283344.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(51340..52188)
                     /locus_tag="VGY24_000331"
     CDS             complement(51340..52188)
                     /locus_tag="VGY24_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ4283345.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLYETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
BASE COUNT        15740 a        11273 c         9468 g        15893 t
ORIGIN      
        1 aattactctt tgctgtttgt gataatttag atcaacactt taataccttt acagatgctc
       61 aaaaaatctt tgaaaatgcc tatcaagatg aaggcctaaa agatgacctc atttctgttg
      121 ttactggtga tttaggttac tttatcgaac cgacactgac ctttgaaaaa ctcatccaag
      181 atgtttatca caatactttc caattagaat cattagctca aggttttcgc gatattgaac
      241 aaagtggtga agactttgaa aacttatttg aagatattga cctctactct aaaaaattag
      301 gctctactcc tcaaaaacaa aatcaaacca tttctaatgt catgaaaaca ttaaatgaaa
      361 ttgactttga ggcagtagat ggggatactt taggtgatgc ttatgagtac ttgattggtg
      421 aatttgccag tgaatcagga aaaaaagcag gtgagttcta taccccacaa gctgtctctc
      481 acttaatgac tcagattgtc tttttaggtc gtgaagatca aaaaggaatg accctctatg
      541 accctgctat gggatctgga tcactccttt taaatgctaa gaaatatagt aaccaatccg
      601 ataccgtttc ttactatggt caagaaatca atacttctac ttataacttg gctcgtatga
      661 acatgatgct tcatggcgtt gccatcgaga accaacactt aagtaacgct gataccttgg
      721 atgcagactg gccaacagat gaaccaacca actttgatgg ggtgttgatg aacccacctt
      781 attctttgaa atggtcagcg actactggtt tcttaacgga tccacggttt tcaagttatg
      841 gtgtattagc accaaaatca aaagctgact ttgccttttt acttcatggt ttctatcatt
      901 taaaaaatac aggaactatg gcaattgtct taccacacgg tgttcttttc cgtggagctg
      961 cagaaggcaa gattcgccaa aaactactcg aacaaggggc tatcgatacc attatcggac
     1021 tcccatcaaa tatcttttat aatacaagca tccctacgac tattatcata ttaaagaaga
     1081 acagaaccaa caaagatgtc ttctttatcg atgcttcaaa agagtttgat aagggtaaaa
     1141 atcaaaacac aatgacagat aaccatatca aaaagatcct tgatgcttat aagagtcgtg
     1201 ataattctga taagttttca tatttagcct catttgatga aatcattgaa aatgactata
     1261 acttaaatat cccacgctat gttgatacct ttgaggaagt cccagttaaa ccactgccag
     1321 aattagctaa acagctcagt gacattgatc aagaaattgc caaaacaaat gccaaactag
     1381 atcaactcat gaaacaacta gtcggaacaa ccaaagaagc acaagacgaa ttggatactt
     1441 ttagaaacta aaagacaaaa aaacaccaca gtagatgatt actactgtgg cgttttattt
     1501 aaaattgagt agtgaacctt tattgagtac ttatcataca agattctcct tcttcaccta
     1561 taaatcaaaa tagaatcatc tcatctaact ttgtccgttt tctttgtttt atttaaaata
     1621 gttatttgaa gttacaatga tttctcaaac gtttagaaag cttaaacacg gttaagagaa
     1681 attttcagga caacttttga tccttccaca gaaaccatgg tgcgaaccat aaagtagttc
     1741 caatcgttga tatataagca tatattaacg ttctgggagt tgtgaagctt tacgagtttt
     1801 tttagacctg gtttaaacaa cttttgtttg actaaattta aaaagctttt catatcaggc
     1861 atttccctac tttttataaa agcgttatct caatcaattt caatcaaaaa gaactcaaaa
     1921 acaatgagtt taaatctggg tatttaacag gaaatcccta gcaacgacac aagtaaaact
     1981 tatgtcgttt gattatctgc aactcagaaa tccataaaat accctaattg ttcggctttg
     2041 tagatagcct cttgcttatt atttaccaaa agctttgagt agatgtgggt caaatgatta
     2101 aatactgttc tcctactaat ttgtaacgtt tcctctattt cttttataga gaatcctttc
     2161 ttactcaaat ttaagatagc tatttcctgg tcagttaaag attcaacata atcctgagat
     2221 tcgatttgtt caaaatactt ttttccagaa tccacttttt ttaagataga aatgagttgt
     2281 ttagggtcta tatttttatc tacaaaaccg tatgctccta ctttttttgc cctctctcta
     2341 tagattgatt taacatatcc tgtcaacata acaactttca aatgaggagt agactgtatg
     2401 agttcttttg ctatctccaa tccattttct ttagaaatat ttgttaaatt aatatcaagc
     2461 aatataatat cgtacttaga caaatcaatc gtcacatcgt taaaatgaga agtaatcgta
     2521 tctatgacat ctacttcatc atactgttgg aataacagtt ggatactttt tgcaaatagt
     2581 ctgtggtcat caattaataa aatcttcata acacaattcc caatctatgg gaagtaatat
     2641 tctgatagta aattgtttag tatccatttg tagttctaga tctccatcaa aagctgctag
     2701 tgtttcttga aatgatttca atcctctgcc ataatttata gagtgtactt tttccactac
     2761 ttgattgctc tcctcaatga taataatatc agattgaatc gttaaagata aacgaatgtc
     2821 ttttccatct ccatatttta tagcattgtt tattagctct tttataaacc tataaacaat
     2881 atctccatat ggggatggca ctaccatatt atctgaacag ttaaaataca ataaggtatt
     2941 actttttcta cttttaacaa tatcatcaaa aagagactgt atgttttctt tcattgtttg
     3001 attagcggga actattgggt ggtaagtatc catttcatct ctaataccag aaactaaatc
     3061 attcaactca ttaacaataa aaccatgtgt tatattacta ttttctagag aaagcaagtt
     3121 ttttatcgct atgatatttt gtaaaacatc atcatgcaaa taatttgaga actccactct
     3181 catgttttcc atagtaaata agcaacatac caccatttac aattccaatg aatacaggaa
     3241 taaggaaaag tatacctgtt tcatatcgaa gaatccgatg aatcattttt ttactataac
     3301 ctagcttact tagatagcca tattcttctt tattttccat gatttcgatg aggcttgtaa
     3361 agtacagaat actacctgta caaataataa agagtatgga taggaaagtt ataaagatat
     3421 aagtcgcaat attagcagtc tttactgtgt attccctact ataactactg ataacatcag
     3481 gaaccatact aaactgattg taaaatgctt cacttgacct aatcgatagc attagtcaaa
     3541 atatcctttg aatttttcat aaaactcatt ctcctttcta tcagtatgta gtgactattt
     3601 tctacacttt tattgtaaat aatacgtctt cattttcaat gagtaaaaag tgcaaaaatg
     3661 acttttttca tattttttag taagattttt gagatgtcac ataagaggtc actttataac
     3721 aaatagccct ctgatataaa aaacaaaact cgttctttac agctatttta caatctaatc
     3781 gcttctcgtt ataacatcaa tctctaatca gaaaatggtt actttactta tttaatcagg
     3841 tcaggagttt gaatgacaga agtcaaatat ctgaaaaagg atgttaaata aaactttcaa
     3901 aacccatttt catcaactaa aaacacttat aacccgatct tacaactaat ggattaccat
     3961 caacagcaaa taatactatg cttgaaaaat gtcaacatca tctacttttt gtgactataa
     4021 ttcaataaac gaacatgacg tgtaaaaatg tattaaagca gtatctcaaa atcattcaat
     4081 cacttgagtt tctgctactt ttttatacca gtaggcactc ttcttaggat aacgttcttg
     4141 agtttcaaaa tcaacataga agagaccgta acgtttttca tagccatttg accatgaaaa
     4201 gacatccatt aatgaccaca tgaagtatcc tttaacattt acaccatcag aaatagcatc
     4261 agaaataact tctaagtgtt ttttcacata atcgatacgt ccatcatcat agacagtatt
     4321 atctacaaac tcatctttgt aaccaagacc attttcagtg atataaattt tcttgtagtt
     4381 aggataatca gctttaacac gcatgatttg atcatataag ccttgtggga agataatcca
     4441 atcccagtcc gtttttggaa catcaacggg tgcttttctt cgaccaacac ccttgatttg
     4501 gtatttagag ctgccttttt cccctttacc attatgaatg atttcagtct caccatcaaa
     4561 agcttgcatc caatcactca tatagtagtt aataccaagg aagtcattca aatcttttgc
     4621 ggcgtctagt gcagtaaaat cttcttcacg aagatcaagt tcaccaccat tcacctcaag
     4681 gatatggtta acaccttcca ttgttttatc tgaatactta cctagataag ttgcatcaag
     4741 aataaattta ttatggatga tatcttccag ttcagctgct ctaacatcat caagattgtt
     4801 agcgtcaaat ggatacttag ttggaagagc atggacaaca ccgatttcac ctgaatagcc
     4861 accatcttta aagagcttca cggcacgggc atgagagacc atcatgttat ggtgtgattg
     4921 gaaaacttta gcaagatcat attggatacc tggagggaat ttaccaacta agtattgacc
     4981 atcgccaatt ggtccaattt cgttaaatgt tgtccaatag ttaacttctg agaattcttt
     5041 aaaacaaaat tctgcataat ttacaaaatg ttcaatgttc tcacgattga ggaaatcacc
     5101 atccgagtgg agagcttctg gtgtatcaaa atggtgaagt gtaacaaaag gctcaacatg
     5161 acgcttatga cactctgcaa aaagattgtg gtagtattct actcctttag ggttaacttc
     5221 tccttttcct gttggaaaaa tacgagacca ggcaatagag atacggatcc cattaacacc
     5281 aaattcttca ctaagtttca aatcgacagg gtaacgatta taaaaatcac ttgctggctc
     5341 agctgtgtac caatagttgt cttctaagta tttatcccaa gctactggtc ctttaccatc
     5401 tgtgtgggta gcgccttcag cctggtaagc agctgtagca ccaccaaaaa taaaatcttt
     5461 aggtaatgtt ttagtcatga tatacctctt ctaaattttc atttcactat aaaggaagat
     5521 tgagtacccc caaccttccc tagcgacata ttatttttcc atttgttctt taacgaagtc
     5581 taatgcgcct tgaccatcgc gagtcaattt gatgtattga gccccttctg ttttagctaa
     5641 tttaatatca agtttatcag tttccacttt catatcttca tagttagaag ctacttgagg
     5701 tgcaaggata acaagttgat attgtggtaa aatctcacgg tgagcaccat aacttcctgc
     5761 tgctgcagtg acaggtgcac catattcttt agctgcttta ttcaaagcgt ttgcaagcaa
     5821 accacttgta ccaccaccgg cacaaagaac gagaacatta gtttgatcag tgatttcttt
     5881 ggtttcttta gctgctgatt tttcaagaat agcatcagct tttttagtat caaagtttgc
     5941 tgctactttt tctttgaggg catctgatga attcgtacct gctgcttctt cagcaagaat
     6001 ttgctcatca taaattttca agaatgggta gtagataatc acatcaacaa caattaacaa
     6061 agctgccaaa atgaatgcta gcacttggaa gtttgtacca aggataagac ccaatggagc
     6121 tggtgttgtc caaggtaagt ttgcagtgaa actgttcatt ccaagtgtgt caatgaagaa
     6181 tttgaaaatc catacattga caattggtgc caagataaat ggaataaaga agattgggtt
     6241 caaaacaagt ggggcaccaa acaaaattgg ttcgttaaca ccaaagaatg ttggaacaac
     6301 agatgcacgt ccaatggctt tgttacgttt tgattttgtt aaccacatga acatgaatgg
     6361 aacgacgaga gttgcaccag taccacccat ggtaacgatg aacatttgag tccctgaagt
     6421 caaaatcttg tcagcatgtt caccagcttg aagtaattga aggttggttt cagcatttgc
     6481 gtaagtaatt gctgcaatgg ctggttcaac aatagatggt ccatggatac caacaaacca
     6541 gaagaaagca aatgcaccga agataatagt gattccaaca taaccatctg ctgcttggaa
     6601 caatggtgca agaagtttac caattgactc ggcaacgtta acgcctaaag tactatgaac
     6661 aatcaattca atcatataaa gcagtacaac tgataatgta aatggaatca aatccttaaa
     6721 tacttgagag atatttggtg gaacctcttc tggcatacgg attgtaacat tgtttttcac
     6781 acaggtttta tagacattta cggtaataaa agctgcgatg aaggctgtca acaaaccttt
     6841 tgttcccata aatccagtta agaaaccacc ttcttttgct ggattggctg ccataagtaa
     6901 aaaacctaca atagcagcga gcatggtaga catgaagttg atttggtttg tgcttggtaa
     6961 tgatcgattc attgagtcag ttaaagcttt ggctgtcgta ccagccacaa acaaacctaa
     7021 aatacccatt gagtagttgt atggcgtcat caagagactc tcaatatcct tactccaatg
     7081 gaagccccat gcatttggaa cataggcaat caagataaag atacttgaaa acaagataac
     7141 tggcatacca gcgataaaac catcacgaat agcgcgaaga tagatatttc gagacatttt
     7201 ctcgaagaaa ggtttccctt tctcaatctg agcaattaat ccattcatta ttttgctcct
     7261 ctcttataaa gttcaatcat gtgtttcatt aaatcttgta ataaaatggt cgtcattaaa
     7321 tgatcttgac catgcattaa agtgacacta taagctaagt catctccagc tgcttctttt
     7381 tgtagtagac ttgtctgagc attgtgagca tcattcaagc agtcactacc tgcagcgatt
     7441 aattcttctg ctttagcata atccccttct tgcgctgctt taagcgcttc caaaaatttt
     7501 gaacgggcat caccagcata ggctacgatt tcaaaaccta aaagagtagt ttcttctcta
     7561 ttcattgtag tttcctccga aaataatgtt acattttttc agtccaagga cttgcagttt
     7621 tatctagtac tttgtttaat tcgtcaatat ttttgaaacc ttctgtacgc aaccattcac
     7681 gagctgcttg tggtccttct tcgatataga cttttacaga tcctgcccag gtagcacgtc
     7741 cacataagac accgttgaat ttagcacctg attctgctgc aaacaccaaa gtatcttgga
     7801 acaatttggc agaaacacct gcactcaagt aaatatatgg taaatcagta gaagcttctt
     7861 ggtcacggaa agcttgagct gcttcttctt ttgtaaagag aacctcacca tctgcaaagc
     7921 tttctacaaa tttcatattt actggaactt cgactttcaa gacgtcaaca ccaaagcgtt
     7981 cttctgagaa gactttcatt gcttcattaa ctttatgagc tttaactttt gcaaactcag
     8041 ggcttgcatt gtcagcaatt ttctcatcat aagttagaat ttctaagtag aatggaatat
     8101 cttctgcacg gcattcagaa ccaatacgtt caatgtaagc ttttttctgc tcgttaacat
     8161 cttgatctcc atcaatatca tagtaaagaa ggaatttaac tgcttctgct ccagcttctt
     8221 taatacgttt tgcagaccaa acatctaagc aatcaggtaa gcgactagtt gttgttgcat
     8281 cgtatcctgt tttctcatat gccaacaaca agcctgcttc ttctgaacga acacgacttg
     8341 ctggcaagcc atattcagga tcaagtaaaa ttgatgatgc aaatggtgtc agttcttcag
     8401 aaactaaact tttaagctct tcaatttgtt ctactgttgg ctcttttgtt tgatgttgag
     8461 ccatcatacg tttgagagct ccacgttggt caaaagctaa agcagaaata acaccatcta
     8521 tacttagttt ttccatgctt ttacgtttgt tttcagttaa agtaattgtc attttttata
     8581 cctctttgac tttaatagat tgatatagct tatcgtaatt tgccatgtta acatgcccag
     8641 ttgttttttc ttgcgcattt aacatcccta agacatttgc ttttgttaat aaagctcgat
     8701 catcttcatc attcgctaaa cctgaagcaa tacctgccac tgtagaatca cccgaaccta
     8761 cagcgcttac aatttcgatt ttaggaatgt caacgttata aaacgtatcc ttgtgctttg
     8821 caaatacacc atcagcacca agagaaacga ttatccattc aattccttca aatagttcat
     8881 cttgtaagac tgcttttaaa gctttaaaat cttttgtcat ttctttacca agaagttgag
     8941 aaagttcttc aagattaggc ttgataactg ttggtttact atcacctttc aatactgctt
     9001 ctaaagcatt tccagaacaa tccagaaccg ttttcttacc attaagattg gcaatcttga
     9061 ttaacttctg atagtaatca tctggcatac cagcaggcaa actgcctgag agcgtcacta
     9121 cgtcaacatc attcagtaaa tatttaaaat gatgagtaaa gccatcaatt tcatctacac
     9181 ttaacatcgg tcctttttct aaaatctccg tttgattacc ttcgtgtaat atggctatac
     9241 atgttctggt atcacctgaa attttataga agcgacttaa aatcgaatca ggaagatgat
     9301 taatgataaa atcaccgctt tctccaccta tacaaccagt agctttgact gtctcaccga
     9361 attcattaag aactcgggaa acattcaagc ctttaccacc aggtgtcttt gtaacatcta
     9421 caactcgatt cacggtatcg aattttagct catccaaagg gtaggagacg tcaatagctg
     9481 gatttaaagt aactgttaaa atcacaatag ctcctttttc tatatatatc cctatagtaa
     9541 tttattcaaa tattagtcgt gatattcacc acggtcccat ttttctagga attcatcaaa
     9601 gaagtgtggg ttgttttgat cttggctagc atgttgcaag ctatccattt tttcaatcca
     9661 ttttttattt tcttcagttg gtttgtattc cgcggctaag aaagcatcga taatatcatt
     9721 catcaataaa ccaccgatga tttttccacc aaaaccaata acgtttgcgt ttagctcttc
     9781 ttttgagtag atagcagatg tcatatctct aacgagtgct gcacgaatac cgggaacttt
     9841 gtttacagca tttgtgatac caacaccagt accacagata acaacaccta attctgcttt
     9901 accacttgct actgcttcac caactttttt accaaagatt ggatagtgtg tgcggacatt
     9961 atcgtatgtc ccgcaatcaa taatttcatg accttgtgat tttaggtaat ccacaacagc
    10021 gattttttca taagtaacaa tatggtcaca acctacagca attttcattt ttgttctcct
    10081 tgttttaacc tatttcaatc aattagcaca ttttattaag catgtcgaca cgaacttgat
    10141 gacgtccacc atcatattta ccttcgacaa agcttttgat aatgttcttt gcaattcctg
    10201 gagcactaat ttcagaacca agcgtgatga ttcgagcatt gttgtgtcct cttgtcatgt
    10261 atgctgaacg ctcatctgat acttcagctg ccaccattcc tttgatttta gtagctacca
    10321 tgaaagaacc aacaccatat gcatcaatga caatacctaa gttatcttcg gatgcattaa
    10381 cttcttttgc aacggctaaa gttgtatcta caaagtcacg tacttcgttt tcactaacat
    10441 caacaacttc gtatttacct tctttaaggt aatctttgat aacttcttta agttcttgac
    10501 ctgctttgtc agcaccgata ataattgcca ttttatttct cctatgattt agaataagta
    10561 aatgtggaaa acctacatag atagttaatt aacgatttgc aacatctttt gaaataacgc
    10621 tttcattatt tctatgacct aatcatacac ccaccaaaca taaaagtcaa cattttttgt
    10681 aagaataaac aaaaaaataa acaaaatatg tttatttttt tcgtttttat gttattatcc
    10741 taatgtttct tgtgtttctt ttccatctta taaatccctt tgacagccac tcgtaaccta
    10801 tcatatatta cgaaaccctt atctctattt attgtaaatg ttttatttaa aacattttct
    10861 attttgttaa tttttgtttt ttccaatgtt tatgtttgtt ttgtttgatt tttttattga
    10921 tattatgtta tgataccttt aatagttata ccctaagaca ctgataggag ataagaatga
    10981 aaaagaaaga gcgtcatgaa aaaattctcg atatcttaaa agttgacggt tttataaaag
    11041 taaaagatat tatcgatgaa atgaacatct cagatatgac cgctagacga gatctcgata
    11101 cattggcaga taaaggatta cttatcagga cccatggtgg tgctcaatat ttggattatt
    11161 cttcagcaaa ggacgaaggt cacgagaaaa cacacactga aaagaaggtc ttacagacta
    11221 ccgaaaaaaa acaaattgca caaagagcta aaaacattat taaagatggc gaaactgtct
    11281 ttataggtcc agggacaaca ttagagcatc tagctgtcga attgaaaaat agaaatatca
    11341 gagttattac aaatagcctt cctgtttttt taattttgca cccgagcact actattgatt
    11401 tgattttaat tggtggtgaa tgtcgtgaaa ttactggagc ttttgttggt gctattgcca
    11461 ttaataatct agaatcacta actttttcaa aggcttttgt tagcgcaaat gctgtttata
    11521 ataattctat tgcaacgtac agtgacctcg aaggagaagt tcaaagggtt gcattgaata
    11581 atgctgttga gaaaatcctt ctagttgata gtaccaagtt tgaaagttat gacttctata
    11641 atttttacca actagatcaa gtggacttaa ttgtgactga taaaaatgtg acaactgata
    11701 tcatagaaaa gtacaagaaa ttctcaaaaa tcataatcgc tgacaagtaa aggacctact
    11761 catattaaaa agcacttcag ggtttagata tatcccctgt caagtaaaca gggagcatat
    11821 ctgaaagtag tgtttgtgtt ataaagaagt ttcttgctaa cttattttct gttttttttc
    11881 agacttatca gtcttgtact gttccttttt ctgatgaata acgtggaaat aggcagacag
    11941 ctatatattt cacctctaga atggttgcca ttactgtcat caagacatgc ttgttcttta
    12001 tcgtatggag taaagctttc ttttagccat caacatacct cttaagtaga ttttggttcg
    12061 ttaccatgtc tacttaagag gtgtcttcaa agcgctttaa aacgttgcta taacagtata
    12121 ttaacgtttt gagaattgtg aagctttacg agctttttta agacctggtt tttggttgat
    12181 attctgatat acataaagat acttataaaa tcaacctatt cctggttcaa tcaatcaaaa
    12241 tctattaatt atatacaaat atcttattcc caacggtatc aataaggtat caaaaataag
    12301 tttcggagct tatcaaataa tgaaaatctg tctcaattac cgagtctttt ttattgactt
    12361 ttttgtatat atgatataat taaataagtt acataaaggg gttacataaa atgactacag
    12421 taaaaaaaaa cagagcggtt acctttcaag ctaataaaga attggtaagc gaagcaatga
    12481 cagtattaaa caaaaaaaat ttaaccttat catctgcttt aagattattc ctacaaaatg
    12541 tcgttgtcac aaacgaggtt gacttattga cggaagagga gttagagaaa gaaaaacttt
    12601 tcaagcaatt tcaagctgaa atcaacaaaa atattgagga tgttcgtcaa gggaagtttt
    12661 atacctctga agaagtgagg gctgaacttg gactataaga aatatcagat tatctatgct
    12721 cctgatgttt tagagaagct aaaagaaatt cgtgattata tttctcaaaa ctattcctca
    12781 acatcaggtc agcgtaaaat ggagcaaatc attagcgaca tagaaaaact cgaggttttc
    12841 ccagaagttg gttttgatgc cgatgaaaaa tatggttcaa aaatcatcca ttatcacagc
    12901 actaaaggtt ataccttaag taaagattat attgtcctat accatatcga gggggaagaa
    12961 aatagggtcg ttattgatta cttgcttcct actcaaagcg actatataaa gttattcaaa
    13021 taacacgtta aaagccttag cagtcttgct agggcttttt tggtagatta ccaattaact
    13081 aggagcaaaa tgagttcggg ctgtttagta gatattccac aaagaagcca aagactacga
    13141 agctaggcta atctacctta aggcttcagg aaacttggag gagttcacca agccaactca
    13201 taaaacttat agcgagatat tcgagaaatg gtatcaagcc tatcaagata cggtagagcc
    13261 aaccacggct tctcgtactc ttgatctgtt tcgacttcac attctgccag tcatgggaga
    13321 attgcccatt aacaaaacct cccctttaga ctgctagaac ttcatcaccg ataaagcaaa
    13381 gacctttaag aatatcaagc aaatcaagtc ctacactgaa aaggtctttg attttgccat
    13441 caagatgaaa ctgctcaagc ataaccctat ggctgaaatc attatgccaa agcggaaaaa
    13501 gacacgaata gaaaactatt ggacggttca agaacttcag gagtttttag ccatagtact
    13561 tcagaaagag ccttacaagc actacgcact atttcggcta ctagcttata gcggcagcta
    13621 tgcgctctta aatgggctaa tatagacact aagcgtcaat aagagcctag gacgattaga
    13681 cggacaggca atcgaaaaaa gcactaaaaa tgatttctca gtaagaaaga ttaagctaga
    13741 cagcgaaact atttctattc tgcaagaatg gaaagccatc agccaaaaag aaaaggcaca
    13801 actagcagtt gcacctcttt caattgagtc tgactttata tttacatact gtactagaga
    13861 agggagtatt gaaccacttc atgcagacta tattaacaat atcttatcaa aggtcattaa
    13921 gcaacatagt cttaaaaaat aagtcctcac ggcttcaggc atacccatgc aaccctgatg
    13981 attgagattg gagttgaccc agtaaataca gccaaaaggt taggacatgc tagtagtcaa
    14041 ataactcttg atacatacag tcatgctact gcagtcggag aagacaaatc aatcaaacaa
    14101 tttgctaact atctcaaaaa taactaaata aacgtattaa accaagtcaa aagttttccg
    14161 aaacggtatc aaaagcaaaa aacgacaaaa aaagcacctt gcaagagact tgcaaaatgc
    14221 ttgtaatcgt tgatgtaaag ctgttcttaa cgttttgaga attgtgaagc tttacgagct
    14281 ttcttaagac ctggtttttt acgttcaacc atacgtgcgt cacgtgtaag aaggccagcg
    14341 cgtttcaatg aatcgcggaa gtctgggtct acttgaagaa gcgcacgagc gataccatga
    14401 cggatcgcac ctgattgtcc accgtaacca ccaccaacaa cgttaacgaa aacgtcgtat
    14461 gaaccttcag ttgatgtaac tgcaaaaggt tggttgatga tcaaacgtaa gtcagcatgt
    14521 gggatgtatt cttctacatc ttttttgtta acagtgattt taccagtacc tggaaccaaa
    14581 cgaacgcgtg caacagcgtt tttacgacgg ccagtacctg catattgtgc ttgtgccatt
    14641 atttaatgct cctttcctct tagataagtc ctgagatgtc aagtacttct ggttgttgtg
    14701 ctgcgtgagt gtgctcaccg ccaacgaaga ctttcaattt cataccttgt gcacgtccaa
    14761 gagtgttatg tggaagcatg cctttaactg atttttcaat caagcgaact gcgtttttag
    14821 agcgaagttc accagcagtg attgatttca aaccacctgg gtacattgag tgagtgtaat
    14881 aaactttatc agtcgctttt ttaccagtta atttaacttt ttcagcgttg ataacgataa
    14941 caaagtcacc tgtatcagtg tgtggtgtga aagttggttt gttttttccg cgaagtacgc
    15001 tagcaactac tgcagaaaga cgtccaagtg gcacatcagt tgcgtcaaca acgtaccatt
    15061 tgcgttcaac ttggcctggt ttagccatga aagttgtttt gttcatgatt tctcctatac
    15121 gaattcgtaa tatttgttta caaggtgatg ggtgttccgt cccatcgaag gtatttggaa
    15181 ggttccgggg cctttcaaat ggggtaaaca ataccgccta ctatgatatc aaacttaaga
    15241 ggctccagtc aagtgcttta gctgatttta ttggattatt ttttctttcg atttaggaga
    15301 atggcagagc tgctctgctg gtgttaacga gaatcatcag cttcccaaaa aaggtcgtct
    15361 aaggtcttat caagcactcg acaaatggca atgcaaagat taatcgttgg gttgtaatca
    15421 cctttttcaa cagccgcaat ggtttgtctg gagacgccta ccaaatctgc tagagcttgt
    15481 tgagacaaat ctttgcctgc acacgctgct tttaatttga ggttcttcat tcatctgcct
    15541 ctttagcatc cagataatga cgatagaaaa tactagctcc catactaagg agcgttatgc
    15601 ctaacaccat cagagtccca ctgccgccag ccttgaaaaa gcttcccatt ttaagttgcc
    15661 ctgccatcat ctctcttacc gccaaaccta tagtgactaa gctcattcct gttgctatca
    15721 tccccattag tattccttgt tttccaaagc gcctatccac aaatggcgat atcccgttga
    15781 ggttacaata tgtcgtttga actgccaacc ctccccataa agccaacgta aagaggaagc
    15841 ttgcagatac cagtccctct gtgaatcggc taataaagag tgacagccag ataaccacaa
    15901 tcataaacca aaacccaata gtgtatcctt tgagaagaat ctgtttttgg cgttcatcat
    15961 atttgggcaa cttctgatcg tcccttacct tagaatctag tacttccttg ctgtctagtc
    16021 tcgtttggag ctttttcgct gtctttagcc cccataccaa caatatgaga aagctagtca
    16081 atataaccgc tacgcctcca actaagtaac gttgaaaact ggttttagcc agcagtgtcg
    16141 ctactaacac aaccaaatcc atcactgcta ttatgattat aaacatccat tgtgacttag
    16201 cgagattttg tttgctatca aacatgattc gtttctcctt taaatcttta catgacataa
    16261 tacaaattat tttggatatt ttgtcaaata tattttacat tcttgattga tttaagcagc
    16321 aaaaaaggat tgaggatttc tcttcaatcc ttataaggga ttaacgagaa ttctgcccaa
    16381 tcacaaaaac tgccagggtg ttaggaccaa cgtgagctga gataactggt cctagtggca
    16441 tcataagaac atcgctaata ttctcatgcg ccagtagctc ttctcgcaac ttttcggcac
    16501 taccctgatc gcttgtataa gagacaataa ccgtcgaatc cgcaatatct ttttccactt
    16561 gagccaccat ttctttgatg gctttttggc gtccccgaat ttttgcaatg ggcaccaatt
    16621 tcccttcctc atcaatccac aaaagaggtt tgatactggc taaactgcct agaaaagctg
    16681 aaccttttga cagacgccca ccacgcatca aatgaaaaag atcatctact aagaaatagg
    16741 tgcgcagtcg aggaatcact gcttcaacaa tatctttagt ctccagtaaa tttttgccac
    16801 tatctctggc ttctgctgct aaaatggtta gatagccttc tcctcctgca gcagccaagg
    16861 tatcaacaat ttcaatcacc gcatcgggat aatcttctcg aacaagatcg cgtgccatta
    16921 gagcgctttg ataggtaccc gataaaaccg aagaaaaagc aagataaagc agtgctttgt
    16981 tgttcttagc atgttcacga aaaaccttct caaactctcc cacattaatc tgactggttt
    17041 gaggatgact gccagctttc atctttttca aaaggtaatc actactaata cggttaggac
    17101 cgaccgtttc ataaacctct ccatcgcaca aaatcgttaa tcctataagg acaatatcat
    17161 gatcttctgc ccaggtttga ttcaaatcag cggttgaatc tgtcattatt gtaaaggtca
    17221 ttcttacttc tcctcatcca gttttttagt ttgataatca aataattatt gttttatttt
    17281 atcaaaaaaa cccgcacttg gcgagtcgta attctcatta tcgatctaac tgagacataa
    17341 aatccttcaa ttgactaagc tgtccttggt caaaaggccg cagtttaggc gttttgagat
    17401 caatatctct agacatttta tctaaatcag ctttgacagt atgaactctt tgctctaatt
    17461 ctcttgcagt gacacgaaga gctccttgag aaaaaatcgt ccattgttca tttaaatcac
    17521 tcgtagctac ttctaccata tgtatagccg tatttaactc tgctgccatg cgctcaatgt
    17581 agctatctgc tgtttcgtct tcctctgtaa ataccactga gatataatac tggtcatatc
    17641 gttgccttaa ccccggtaca tattgggcat caaaaacaca aataatatta atattctcaa
    17701 aatgggcata atgattaagt tttgttaaaa gtgtgttacg tgcctgatca agctgatttg
    17761 tcttaaacaa ctgacgggtt gattgccaaa aggcaatcat attataccca tctactaata
    17821 atatccgttt tttcataatt tagcgcctat ttcggaaaac ttcatacatg agaatagctg
    17881 ccgctacgct agcatttaag ctctgtacgt gaccattcat aggaatcgta atcatttcat
    17941 caacttgctt tttgatattg gtagagatac ctttgccttc gttaccaatg actaaagcaa
    18001 gtttaccatt agtgttccag caatcagacg gtgttccatt catgtctgtg ccaaaaatcc
    18061 agaatcctct tgctttcaat ttatctagag tttgactaag gttagttact ctcgctatcg
    18121 gaatatgttc aacagcacct gtagacgtct ttgagaccac tggagtcaca ccaacagaac
    18181 gatgtttagg aataatcact ccacatacat ttgtagcatc agctgtccgt aaaatcgatc
    18241 ctaaattatg aggatctgtc agaccatcta aaataagaat caaaggattt gcttcttgct
    18301 ctgctatttc gaggatctca tcaacatcag tataggcaaa agctgatact ctcagaacaa
    18361 aaccttgatg cacagctcca tcagtcattt gtgacaaggt ttttttaggc gtccatgaaa
    18421 tagcgacctt tttttgtgtc gctaagcttt tgatgttatc cactttcttt cctcttaaat
    18481 cctcttggat ataaagctta tttcctgtat ttgcttgaag gctttctgta acggcatgaa
    18541 caccatagac gatatcgttt gtttcaatag tatctttatc ttccatgagt ttattatacc
    18601 atgaatgctt caaaataaat gcatagactc ttttattttc gtctagatac tttttgaata
    18661 gctataaata aacctgcagc taaactgact actagacctg ataagaccca agggaatcct
    18721 ctttttgaag taggttttac ttcctctttg tccactagca tttttgtctt gttgctaccg
    18781 tttttttggc tattactagc agttggtgta agtttctcat ttttagtaga tcgctttaag
    18841 gaagttgatc gatgattagt agctgatttg aaaggatttg aagtagaatc tatttcttgg
    18901 ttagctgatt gatttgcttg agcttttgtg tctacatcga ccgaattcgt cgttgcttta
    18961 gaagtatcct tttgttgcat attagttggt gtttttctag ctttgttttg atcaaaatga
    19021 acagttcctg attgaccagt tactgccttt tcttgagtat gaacggtgac agttacatga
    19081 tagtcaccac tcttttgtcc agttaaatca aaccaagctg taaaacgacg ctgttgcgct
    19141 accatagtat atacccaact attaatagta ttatcactag cgtctttaag tgttatcgtt
    19201 gcctgagttg gttgtctgac aggttgatct gtggtgactg tagcatcaat ataaggttct
    19261 gttccacgca tactggcttc aacactgatc tgactaatag ttgattcagc tataacatta
    19321 tcagtgtgct gatcttgtgc atacacagat atggctttgc cagaagtccc cagcaaacca
    19381 ataagtaaag ctagtgataa taagctaact gttttttgca tagttgtcat aataaatatc
    19441 tcctctttaa attagtctta ccctctcatt ataaactatt cgaaaatgat ttcattattt
    19501 ttatgtcttt tttgttacaa atatatcttt tttatatcat tatgtctttt tgatgtcata
    19561 aaaagagtta atggctctct acctttactt cagacctatg aaaataagag tctcctaggg
    19621 gactcttatg aggatatcaa ttgttgcttt tctacatact caatacacca tctgatcaat
    19681 tcttctaacc gctctttttg gcccatcata tcaagataac ccataatggc ttcaaaacct
    19741 gtcgacatac gataggtaat aatatcggca ttcttagctt tagtatggct attggtattg
    19801 cgaccacgct tatagatgtc ttcttctttt tcggtcaata gctgcgcttc taacatagcc
    19861 tgaatcaagt tggcttgtgc cttagcagaa acatatctcg ttgctaaacg gtgtagctgg
    19921 ctaggtttcg ttttaccttg aaaaatgaga tgacgacgaa catagtagga ataaaccgca
    19981 tccccttcaa aggctagggc aatgccatta atcaaattca catcaactgg attagtcacg
    20041 cagccacctc acaccatctt tggtatctag cagcttgatg ccttgagctg ctaactgatc
    20101 tcgaatggca tcagctgtgg caaaatcacg attcgcgcgt gcttcttgtc gcttggcaat
    20161 caaggcttca atgtcaacct caagcacctc ttcttcaaag ataatgccaa aaacagctaa
    20221 cattttctca aaagcgcttt taacaggttc tgtataggag ccagaattaa tccacttggc
    20281 catgtcaaat acgatggtaa tcccatttgc tgtattgaaa tcatcgtcca tagcgtcttg
    20341 gaaagctatt acaaactgtt ttagctcttg ctcatctgca gtttctgtca gcggttgttg
    20401 caaggtgttt ttaagatact tcagattgat ttccgcatca tgaagagcct tttcagtgaa
    20461 gttaattggt tttcggtact gttgtgtcgc aagaaagaat cgcaaaacct gaccatcaac
    20521 ggtttgtaac atgtcatgaa cggtcacaaa attacctagt gacttggaca ttttttcgtt
    20581 atcaacagtg acaaacccat tgtgcatcca atagttagca aaagtcttgc ctgttttggc
    20641 ctcagattgg gcaatttcat tggtatgatg aggaaattcc aaatcagctc cgccaccatg
    20701 aatatcaatg gtatcaccaa gaatttcagt agccatcaca gaacattcaa tgtgccagcc
    20761 tggacgacca aacccccagg ggctatccca agaaacctca cctgccttag ctgatttcca
    20821 aagagcaaag tccagtggat tttcttttaa agctgtctca gcatctgttc gaccactggc
    20881 tccaacttca agttctgaca gggttttatt agctagcttg gcataatgct ctgacttttc
    20941 cacgcggaaa tacacatctc catctgcttc gtaagcaaaa tctttttcaa tgaggctttc
    21001 aacaaatgaa atgatttctg ctatataatc cataacacga ggattttgtg tggctggctt
    21061 aacaccaagt gccttggtat cttctataaa agctgcaata aagcgatctg acaattcttt
    21121 aggagaaaca cctgcttgag tagcagcttt gataatttta tcatcgacat cggtaaaatt
    21181 ggaaatgtaa ttgacctgat agccaatata ctcaaaataa cgtcgaatgg tgtcaaaagc
    21241 gaccgctgat ctagcatttc caatatgaat ataattatag accgtcggtc cacaaacgta
    21301 tatattgact gtattttcag tcaaaggtac aaacttgcgg agcgaacggg tcatggtatc
    21361 ataaatttta atcatgtaaa ctccttattc aatagtctgg cattagttgt catcttgata
    21421 tcctaagtct ttttctgaaa tgctgttcca ccttttcaac aatttgatat taataaccaa
    21481 acctattaac caaaataggg tcaaaatgaa gtatataatc gctttaacca tgtcttgtct
    21541 cagtaaaaag attagactag ccaccagtgt aatactagaa acaaggatag acaaactcat
    21601 aatggcatac agcccaactc ttattttatt catgaggctc ctttattaac gaaaaacctt
    21661 cacaacctgt gaaggattca gaaagtagac aaactcactt ttatgctcaa aaagcatgaa
    21721 tagatctgct gatttgacat caggtttcct atttctgcat gtggggtaag ccaaaacagc
    21781 tgctttttgt tgtggtaaaa gtggcatcaa tactgacagt gatgcccata cggatgtttg
    21841 agaccttagg ctcaaaacta aggcatagaa ttccaaagga agtcgctgcc gtaaacacca
    21901 cctgagaaaa agtatcacaa tagactgttt cttcagtcta agacttgctt agttgatgct
    21961 cttttacgcg ttttttgtat tttgtaacat tcccatatca ctacactcag caaccaaact
    22021 gatagggctg gttaagttca acattactct actagaaagt cccaaaatat tttattggaa
    22081 ggcatcagtg cttctttttg ttattttccg atcattttga caactgataa gagacctctc
    22141 gttgtttttg taaactttga atttgacgat tatctttttg cccatgcact cgtactatct
    22201 tagctggcac acctacaact gtcacgtctt caggaacatc tgataacaca acagctgctg
    22261 cccctacttt agcatttgct ccgatgtcaa taggtccaat cacttgggca tgtgccgaaa
    22321 ttaaggcacc ttgtcgaatc gttggatgac gcttgccaca atcttttccg gttccaccta
    22381 gggtcacccc atgataaagc atcacacctt tttcaacaat tgctgtctct ccaataacaa
    22441 gaccagcacc atggtcaatg aagactccag gagcgatttg tgctccagga tggatttcaa
    22501 tttgtgtcca aaatcgccaa aattggctat gcattctggc tagtaattta aaatgatgtc
    22561 gccaaagaaa atgagacaaa cgatgagcag ctagggcttt gatgcctgga taagttagga
    22621 taacctcaag actattacga gcagctgggt ctagagcttt aacaatagct atactttctt
    22681 tccaccaacc catactaact cctacgactt atctaattct gaccaatcaa caagaatttg
    22741 ttggtcatca aaaggattga tggcgacacc gaacgtttca tcaagctcgt tttcaccatt
    22801 tcttggttga taaatatcat caatcgtcca agtcagaatc actccttcag ctttcctgaa
    22861 aaggccgcca aagtcatctt ggttgtacca cttagcaaag ctttgaagat ttgaaaaggc
    22921 aggaacatag ctttttcctt caggcgttga catggtagga aaaaggcgat cgtaatggtt
    22981 atcatcttta ggataaacaa aggcaggaac aaggtaaacc ttttccatcg tatctgccgc
    23041 cacattgtca tcactcataa gcgtatttaa cacagtgatg taatggttca taaattgaat
    23101 catgtcactg cttttgaaaa tggtagaatt cccaaaatct ccctttttct taaggttaaa
    23161 gacaagacct gctgcaccag atgtaatgac ttcttctagc actgcaagag ctgaacgctc
    23221 cagccaatac tgactttgag cactcttttg ttcttcttta aagagagcca tatcttcctt
    23281 atcggtaaaa acaggggtaa ccttgacacc ttcgacttct atcacgtaga gttctttagc
    23341 agcccagaca ggaaaattat ggaaagcgtt gacaagcgca aggctatcca aaaagttatc
    23401 aggcgcattg ataaaagctc ttaagcgaat atctaattca tttgatttag tcatgttttt
    23461 cttctttttt ctctggtttt ggtgggcgtg gaatcaaagc tttcattgag gcatccacac
    23521 gacccttctc atcaatctta atgaccttaa catcaacgtc ttcacccact tcaaggacat
    23581 ctgacacgtt ggtcgttcta gtccaagcaa tttcagaaat gtgaacaagt gcatctgtct
    23641 tgtcaaagag gttgacaaag gcaccaaatt tctcaatacg gacaacctta gcatggtaaa
    23701 cttcgcctac cttagcttca cgtactaggc cagcgatgat ttctttagtg cggttaatgg
    23761 cagcttggtc acttgagtag atagagacat ttccttcgtc gtcaatatca attttgacac
    23821 ccgtttcggc aataatctta tcaatggttt cgccaccttt accgataacc actttgattt
    23881 tgtctacgtc aatcttgatg gtgtcaattt tcggtgcagt tggggctaac tcaggacgtg
    23941 gttcagcgat agttgcttca atcacatcaa ggatttcaaa gcgagctttt ttagcttgtg
    24001 caagagcttc ttctaggatt tgaggggtga tccctgcaat cttaatatcc atttgaaggg
    24061 ctgtaatccc ttcacgagtt cctgccactt taaagtccat atctccaaag tggtcttcca
    24121 gaccctgaat gtcagtcaag acagtataat ttatcccatc ggaaatgaga cccatggcaa
    24181 tccctgccac gggcgccttg attggtacac caccagccat taaggcaagg gtaccagcac
    24241 agatagaagc ctgagaagac gaaccgttag attctagaac ttcggcaact aaacgaatgg
    24301 cataaggaaa ttcttctaag cttggcaaga cttgttcgag agcgcgttca cctaaggcac
    24361 cgtgtccaat ttcacgacga ccagcagctc cgtaacgacc cgtttcacca actgaatatt
    24421 gtgggaaatt gtagtgatgc aaaaagcgtt ttttgtattc tggagcaaga ccatcaatga
    24481 tttgagtttc tcccattggt gccaaggtca aaactgatag cgcctgagtt tgaccacgtg
    24541 taaagagacc tgatccatgt acttttggca aaaagtcaac cacagcatca agcggacgaa
    24601 tttcgtcaat cttacgaccg tcaggacgaa tcttgtcctc tgtaatgagg cggcgcactt
    24661 cagcatgctc catttgttca aggatttctg ccacgtcacg cataatggtt actaggtttt
    24721 cgtcttctgc gtaacgctct tcgtactcag ccttaaccat ttctttaacg gcttctgtgg
    24781 ctgcttcacg cgcttttttc tcttcaactt gaacagcttt ttgcagttga gcattgtact
    24841 tagccacaat atcagcttgt aagtctgcat caacctgtaa cagttcaacc tcagctttct
    24901 ctttaccaac aactgctacg atttgttctt ggaaggcaat taattcttga atggcttggt
    24961 gaccttttaa aagagcctca agcatgatgt cttctgataa ttctttggca ccagactcaa
    25021 ccatattgat ggcttctttg ctaccagcaa cggttaattc taagagcgac gcttccattt
    25081 gttctttatc tgggttgatg atgaactcgc catcaatata gccaacttga acacctgcaa
    25141 tcggtccatt aaatggaata tctgagatgg acaaggccag agatgagcca aacatcgctg
    25201 ccattggagc gctagcattt tcatcataag aaagaacagt gttaatcact tgtacttcgt
    25261 tacgaaaacc ctcggcaaac atgggacgaa tcgggcggtc aatcaagcga gctgtcaagg
    25321 tcgcatctgt tgaagggcgt ccctcacgct tcatgaaacc tcctggaaat ttaccagcgg
    25381 catacatttt ttcttcgtaa ttaacttgaa gagggaagaa atcacccgtt gccattttct
    25441 tagacatgac agctgcggtt aggacagttg agtcgccata acgaacaacg gttgccccat
    25501 tggcttgctt agcgacttga ccaacttcaa caacaagggg tttccctgca aatgttgttg
    25561 taaaggtttg ttttgacata tattctcctt ttaattttca gaggggtaaa gaggtacctg
    25621 ttctctgctt agattcaatt gttatacgtt gttttgctac aaaaagcaac ctgtcaggct
    25681 tgtcacaagc tcctctttgt atcaaacgac gtatctgcta cattttacca taaaatagca
    25741 gaaatttcac tgtcagatcg caatctcttg aaatgtcctt gaaaaatatt aaacgaaact
    25801 tatactcttt aacaaagcaa aaaaagcagg ttggtatgcc tgctttcgtc tcctaatatt
    25861 aatgattgtc atcctctaaa attttaaagc cgaggctggt actgtctttc agaaacattt
    25921 gcacggtttc aagggaatag tcttctaaat cttttccttg aagaggcaac ttggcaatca
    25981 aatcagtggt acaagtgata atgtccacgc ctagctgatc agcttgatag atattataag
    26041 tttcacgagg actagcccac aacaattcga ccccttcttt ttgacgacaa atgcgcagag
    26101 cttcttccat gattggcatt ggatcaacgc cggtgtctgc aatacgacca gcaaaaacag
    26161 acacaatagc tggcactcca gcggttaaat gatcaaccac agcttgagtc tgctcaattg
    26221 taaaaatggc tgtaacgttt aacttaatgc ctttggctga caacttttga ataacgggac
    26281 aggttgattc tcctgttgaa gtggtgactg gaattttcac atagacattg tcacctagac
    26341 tggcaatttt ctcagcttcc ttttccatgc ttgcgagatc atccgcaaag atttcaaagg
    26401 acactgggta gtctgtaatt ttggcaagca cttctttggc aaagccaata taagaactga
    26461 tgcctgcttt tttcatcaaa cttggattgg ttgtaaagcc tgtgaccaaa cctgtttgta
    26521 aatcctttaa catggtttct aagaccgcac cgtctgaaaa gactttaacg tttagttttt
    26581 ttgtcatcat atcctccttt tagatttctt ctgaaacatt attgatggcc tcttgagatt
    26641 ttgacagaaa gtttggaagc accaatacaa tagcaagcac agcaaccacc aagtaaatac
    26701 cgatttgacc aatcgacgtt cctaagcggc ccagtaaaat ccctaaaaca ccgaaatcca
    26761 catctccaaa ggtcgtgttt gaaaaaccaa gtttaccaag tacaggtaga agcatggctg
    26821 gcaagaaggc gagtaataag ccattagcga aagcaccaat catcgcaccg cgacgaccac
    26881 ctgttgaatt tccaaaaatc cctgctgttg caccgcagaa gaaatgaggc accatacctg
    26941 ggataatcag gacaccacct gcaactccta aaatcaacat cccaagtaag ccaccaacga
    27001 aacttgacgc aaaaccgatg ataacagcgg ttggcgcata agggaagaat accgcacaat
    27061 ccacggctgg aatagcattt ggaattaatt tgttagcaat accttggaag gctggaatca
    27121 agtcagccaa aatcatgcga acaccggcgt agacaattgc cacaccaacc gcaaaggtaa
    27181 gaccactctt aatggcaaag ataaatgggt tttgacctgc tgctaattct tctgttactg
    27241 aagcattcct caaaacagaa gccactgttg ccaccaagta gaaaatcacc atgataagtc
    27301 ctgttgaaat ggtggtgttg cgtaagaaac tccatttttc agaaatctga aggtcctcgg
    27361 tatctttact gtctttgcca accttgctac caacccaggc agaaaggtaa tagcccaaac
    27421 taccaaagta tcccatggcg atttcatctc cgtcagtcac tttcaacgta tactgttgac
    27481 caatggctgg cgaaatagct gaccaagctc ccaagaggaa cccacctaag ataatcaaaa
    27541 gacttccttt gaaaccaaca gctccaagca cggcggacaa gagacaagcc ataaagaagc
    27601 tgtgatgtcc tgttaagaaa atgtatttga agcgggtaaa acgagcaaaa gtcaaattga
    27661 gcaataaccc aatcaccaag atggacatag tttccacacc cagaatcttt tgagcgacag
    27721 aagtgacggc ttcgttattt ggcactacgc cagtgatgct aaaaccatgc tcaatgagtt
    27781 tggcaagagg gtccagattg gtcacaataa cacccgcccc tgcagcaagc ataaggtatc
    27841 ccaaaatcgg ccccaaggtt cctgtcaaca ccttatgagc aggagttttc aaagccacca
    27901 accctgcaaa ggcaatcaag cccattaaaa atgcaggttc ttttaaaata tctcgaataa
    27961 atgataataa tgcttccata gtatcttcct atctccttta aaggcttatt ccttgccact
    28021 catttccttg agaaccggaa ctaaatcttc ttcaattttt ttacgattgg tatagctttt
    28081 aacgatacaa accttggcat cctctggaaa aatatcttta aattctttaa cggtaacaaa
    28141 taaatctgca ccctttccaa cagccgcatt ggaatcacag gattctgcat ccacatcaat
    28201 acccaaacta gaggcgatag cttctacttt catgcgaagt aacaagctac taccaatacc
    28261 gtttccacaa accgttacaa ttttaatcat gtgattcatc tcctttttca atcatatggc
    28321 taatcagact cattatttcc tcagtattct tagcctcaat taattgctca atatgttcat
    28381 cgtcatctaa aatcaacgac agttcctgca aagccttcaa atgtgaacta gaatccacag
    28441 cagacaaaac aaaaatcaat tgcacttgct tgtctggatc accttcttgc ttcaaatcaa
    28501 agctgacagg ttcttttagt tgtaagagag acatgcctaa ctgccgcgtt cctttttcag
    28561 gcgccgcatg aggaacagcc accttagggg ctaaaacaat ataggcccca agctcattga
    28621 ccgaatcaat catgccatcg atataagatg tttcaatcat ctggtgttct agtaaaggct
    28681 gagcagccaa acgaatcgct tcctgccaat ccgaaacagc atcagcctgc ctaataaaat
    28741 ctgctgttaa caagtccaat agtcctagac ctcctttttc aatcgttaaa tggttaccaa
    28801 tcaggtaacg ggataaatca ctaacaagtc cttctttatt agtaatcgtg gtgtgtttat
    28861 gaataatact caacagatca tccaaagcga attgctctga caagggaaga tgaaagtcat
    28921 cacagaccat ctttttcaac atcatttttt caacaggtcc catcaaagct tgcgtcacat
    28981 aaacaggttt agcacagtca aaagctactg ttgaaaaaat caaatcaaag gatgctggat
    29041 ccaacaactt tattttgtcc agctggtgaa tcctaataaa ctgtagctgt ggaaaaagtt
    29101 ccttcaaggt tgcctccaac atcagagacg aactaatgcc attagggcaa acagataagg
    29161 ctaccagctg attgcttggc ctcttcttag gggcctgcaa ccatctccca aaatgaatgg
    29221 taaaataagc aacctcatct tcattaactg atttccccaa ttgtttttct agaggagaaa
    29281 ggctgcgctt gaccaagtaa aagagagatt cataatcttg tttgatttgt tccttcaaag
    29341 gattggtcaa gttaatgtcg tagtaaagtc ggtaataagc tggcaaaagg tgactgtata
    29401 ggttctgacg aagttctggg gtatcggtga tagacaatcc cgtgttaacc gccaccgaat
    29461 tgatgatttc atccataatg tcataaatag gttgctgaaa gactaactct aagtcacctt
    29521 ggatacaacc aagtaaccta gattgaacca aatatttttc ttcggttaga ctggggaagt
    29581 tgaccaataa ctggtccacc atcgtttcaa cagctggcga agctaaagga aaagtatcca
    29641 ctgcttcagc ccttacattc cgtgaaaatg gtcgatgagc gagaaggcag aaaaaataaa
    29701 tcaaatccct caatttctcc gaaataaaag ttaagtggtt ctccctgctc agttcctcca
    29761 acgttgctgc catcaccttt ttctggtcgg gcaaaccaag tttatgcaat aagtaactga
    29821 acacccaagg tcctgatgtg acttgcaaaa gagaactgac cgctgattct aagagacgcc
    29881 gcaaggctaa gggttctcct tcaaaaaaat aaccatcctg gcgattgtaa gctaaagtta
    29941 cctgaaaagc ttgtacccga tcacgcacca agtttaaatc agcaataacg gaatttctgc
    30001 tgagccgtaa caagctttcc aagtggttaa tggagataaa ttctttagcc atcatgatgt
    30061 aaatgataat catgtccaga cgctcatctt gaaagagata gtcgtctaag gtaggtagca
    30121 tgcgattcaa caagtccttt tcagtaaggt ctgacggata aaccagctgc ctgtccagag
    30181 caataagggg taactgatag agtgacaacg tgtcattgat tttagcaata tctgccagta
    30241 tatgacgttt gcttgtcttt aatatggatt caaatgcctc cagtgaatgt cctttatatt
    30301 tagaaaagag ttgatagttt ctgatgagct catgagatag catgtcatct cctcctataa
    30361 tgtcagtata aagtgaagcg ttttcatttt caagacaaat tgagtccaat tgtccgtagt
    30421 catttttgac tgcaattgga ctcaacaggt gatatcattg taacaagttc ctcaaaaggt
    30481 ggcccaaaat aggcaaatct gggaattgat tccctttttc ttcatgatac aacaaaagat
    30541 acctcatcac tgagatacct tttgttgcta aataaaatca tcaactgcta aaaaaacagt
    30601 cttagaccat tctagccaaa tgtgaaaact aacaaacaac atgtcaaaaa tgaaaaagcc
    30661 cactgcataa gcagaggact ttttaaactt gaaattaacg acgaagtcca agagattgga
    30721 tcaactcacg gtaacggtta acatctgtac gacgtaggta tgccaacaag ttacgacggt
    30781 gaccaatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgc tctttgatgt
    30841 ggctgttcaa gtggttgatt tcccaagtaa gaactgctac ttgaacttca actgaaccag
    30901 tatcgccttc gtgacgtgca tattgagcaa tgatttcatt ttttttctct tttgagattg
    30961 ccataatata ttctcctttt tttgcttaat ccgagtctta ggattggcac tcctaaaacc
    31021 aagaaaaagt tggtttgtct ttcgaccact ctattctagc agattcataa tattaggtca
    31081 accgttatcc tattattcta agctctcaca tccaaaccac ctcactaggc ttaaaaaggt
    31141 tatctggtcg ttgaatgtgc tctttttgct ttaaccaaac caccataagc aacagaacta
    31201 gcaaggccaa atagccaata ggctgttttt aaatcagaat aaacctgtgc gattgcttgc
    31261 ctaataagat aaccagctaa aagaggtaag atggctgatc cccgcacttc agctgataag
    31321 cggtaaccta gcatagtcat tcatttcttt ccaacaaagt gttcactgat aatattaatt
    31381 gctctagtat taacaaaccc aatccctaat ccatgaacac agcttattaa ccgatgcgac
    31441 ctgcctttat gacataggtc aagagttgac acaaaatgtt tgccaacaaa acgttaccta
    31501 taccgtcaaa gagcgctttg cttcctatac ccttgaagcg caagccaacc aagaggttca
    31561 tctaaacctt acattattag caaaccgttt tggaacaagt gatcgccatc ttaagcacgt
    31621 acttaagcag cccatttttc agcggattat cgaacgcatc tgcttcaaaa cctatcgtat
    31681 tgccgattta gacgctctaa ctcaattaag acctctgagg tatgcagccc attaacttat
    31741 aatagcaatc actctaacca ctatctgact tttctttaaa aaagaaatga tttgtgatat
    31801 agtaaatcca gttaaaaaaa agtaaaggag tagacatgtc tgcacaagat aaattaatta
    31861 aacctagtca tctgattacc atggatgaca ttattcgtga agggaatcca actttaagag
    31921 ctgtcgccaa agaagtgagc ttacccttgt gtgatgagga tatcctatta ggagaaaaga
    31981 tgatgcaatt tttgaagcat tctcaagatc ctgtaatggc ggaaaaactt ggtcttaggg
    32041 ccggtgttgg tctagcagct cctcagattg atgtgtcaaa acgtatcatc gctgtgctcg
    32101 ttcctaatct tcctgataaa gaaggaaatc ctcctaaaga agcctatagc tggcaagaag
    32161 tcctgtataa tcctaaaatc gtttctcatt ctgttcaaga tgcagccctt tctgatggcg
    32221 aaggttgttt atctgtcgat cgtgttgttg aagggtatgt ggttcgccat gctcgtgtta
    32281 cagtagacta ctatgataaa gaaggacaac agcatcgcat caaattaaag ggatacaacg
    32341 ctattgtggt tcaacatgaa attgaccata tcaatggtgt tttgttctac gatcggatca
    32401 atgctaaaaa cccctttgaa accaaagaag agctcttgat tttagactaa caaaaaaagg
    32461 ccctttagcc tttttttgtt agtctaaata gccgctaaaa gagcctctgc ctgagatagc
    32521 aagtttgctc ttgtctcttt tgaaatctca agccttcctg ttccccaggc atcaggattc
    32581 acagttgctt ttgtaaactc tcctgctact gaagttcgga taaacggcaa tagtgcttta
    32641 aactgatcaa atacttgatc atgcccgcca tttgcaactg aagagaccgt aaccacctta
    32701 ccgccaatag cagatgggcc cgtcggatca gacaaatcaa gagcacgaga caaccagtct
    32761 agcaggtttt taacagaacc tggaatagag aagttgtaaa ctggtgtaaa aatccagata
    32821 gcatccgctg actgaacagc ttgacgagcg tcaacaactg gtaaaggtgc attagcttcg
    32881 atatcttgat tcaaaacagg aacgtctttc caatttaagt aagatacaac tgcttgatgt
    32941 tccagagctt tttgtgcttg agccgctaat tgatggttaa aagacccttc acgaagcgag
    33001 ccaacaataa ataaaatatg tttcatctct ttgccctctt tttttaaaga tcgttttaaa
    33061 gctttctaaa agtggtattt cattgatact tctttttgaa atttatcact atgtagtaat
    33121 tatatcttct ctaagaagag ctgtaaagag atttgtttat aattgtttga actttttgag
    33181 aagttcaatc agacaaagtt gttctttttc agtcaaaaca gcaaaggctt cttccacccg
    33241 tgcgacgtgt ttaggcaaaa cagcttcaat caagcgagta cccttatctg ttaaggtaac
    33301 cacataggct cttttatccg ttttgtcttt gcacttgcta atccagccat tgcgttccat
    33361 attatttagt acaacagtca tattccccga agtcgctaac aaagaatcaa tcaagtgatt
    33421 gattcgcata cagcctttag tatatagtac ttcaagcaca ctgaactgcg tggctgttaa
    33481 atctgccttt ttaaaaatat cagccccaaa agcatctagc gttcgctgag ctttacgaaa
    33541 caccaccatg gctttcaaag cggtgttctt atctaaatga ctcatttgat ttcctcgctt
    33601 tatacggtat gtcacagttt agtatcagag agattagaaa gtgatcttgt ccctcttctt
    33661 ttatgacttt gcatattact atcatcatga taatctagaa aaagtcatca aaaagactta
    33721 attgattatc ttctggcata tttcctaaaa tacccatctc atccattttc tcaacgagcg
    33781 ttgacgatgc cccgcctcgt ttacgcaatt ccattttaga gaggaattcg ccttcttgac
    33841 gagctttaac gatttgcttg gccacgtttt cacccagacc ttctaacgct ataaatggag
    33901 ggataagggt atctcctttg atttggaatt ctatagcatc acttttgtaa aggtctaatt
    33961 tgccaaactt aaagccgcgt tctaacattt cgttgacaat ctccaaggtt gtaaagaggt
    34021 cattttccac attggtggct tcattatttt tacgttttat agtaatatct tccattcttg
    34081 ctttaacagc atctaaacca ccactcatgg tttttaattc aaaagccttc gcacgaatag
    34141 agaaataagc acaataatac ataatggggt ggtgcacctt gaaataagcc acccgaaggg
    34201 ccatcaaaac ataagctgcc gcatgggctt tagggaacat gtacttgatt tttccacacg
    34261 attcaatgta ccagtcgggc acattgtttt ctcgcatggc atcaatatag ccattacgtt
    34321 cttcctcaga aatttttagc cataatccct tacgcacacg ctccataatg gtaaaggcca
    34381 tttttggttc taagcctgcg tgcatgaggt aaaccatgat gtcgtcacga caaccgataa
    34441 cggtttttag ggtcgcaatg ccttctttaa tcaaatcttg tgcattacca agccaaacat
    34501 cggttccatg agatagtcca gacaactgca aaagctccgc aaaagtggtc ggatgcgtct
    34561 cattaaccat gccgcgaaca aagttggttc caaattctgg aatgcctagc ataccagtcg
    34621 gtgtcccaat ttgttccggg gtaacgccca aaacctctgt cccagaaaag agagccataa
    34681 ctcccggatc atcagcagga atagtaatag gatcaatgcc cgataaatcc tgaagtttac
    34741 gaatcatggt cggatcatca tgccctagga tatcaagttt caagacgttt tcatcaatat
    34801 catggaagtt aaagtgagtt gtctgccaag aagccgttac atcatcggct ggatattgca
    34861 cgggggtaaa atcataaaca tccatgtaat taggaataac aacaatcccc ccagggtgct
    34921 gcccagtcgt tcgtttcaca ccagcagcac ctgctgctag acgatccacc tcagcatcac
    34981 gatagaactt gccatagtcg cgttcataac ctttgacaaa tccataagct gttttttctg
    35041 ctacggtacc aactgttcca gcacgaaagg cgtattcgtc accaaaaata tctcggacat
    35101 ccaaatgggc actgggctgg tcatcaccag agaagttcaa atcaatatcg ggcaccttat
    35161 ccccatcaaa cccaagaaag gtctcaaagg gaatgtcttg cccatctttt tgataagggg
    35221 tgccacattt cggacagggt ttattaggca aatcatagcc agatccaact gacccatctg
    35281 tgataaattc agaatgttgg caggacgggc aaacgtagtg aggcggcata gggttaacct
    35341 cagtaatccc aatcatggtg gcgacaaagc tagaccctac agatccccta gaaccaacta
    35401 ggtagcctcg ctcatttgac cggttaacaa gcatttggga agcgagataa atcacagcaa
    35461 aaccgttccc caagatagag gttaactctt tttcaatgcg taaatcaata atatctggga
    35521 gaggattacc ataaatttca aaggcttttt gataggttaa ttcggcaacc gtctcttcgg
    35581 ccttgtcaat atacggggtg taaagatcgc ccttaaccac ttccacttcc tcaatacggt
    35641 ccgcaaaatc ctgagtattt tgcacaacca cttgataagc gaggtctttt ccaagaaagg
    35701 caaactcatc cagcatttca ttggttgttc taaagtgcgc tttaggtaga ggagcaggtt
    35761 gggccccttc cccacggccg attgttctat taatcatggc accctgaccg agactacgca
    35821 caataatttc acggtaaatc tcttcttcag gctctagata atgcacattc ccagtggcaa
    35881 gcacaggttt cttagctcgt ttccctactt caatgaggtc acgaatcacc tgctcaatac
    35941 ctgcttgatc tttgattaat tcacggacaa ccaatggctg gtaaatggct ggtggcatga
    36001 tttcgataaa atcataatac ttagccaaat caaccgccgc atcaattcct ttagtcagaa
    36061 cggcatcaaa aacctcgccg tcagaacaag ctgttcctag taacaacccc tctctgtgag
    36121 catctaagac ggttcttgga atacgcggca ccccttcaaa atatttgata ttggaaaggc
    36181 tgaccagctt aaacatattt ttaagaccaa cctgattttg cacatagata gtcgcatgct
    36241 taagccgcgc ttttttgtaa gaatcctcag ccaccaaatc tgtattgagt tgcaaaagat
    36301 ttttgatgcc atgcttttct ctggcatctc ttagaaaaat aaacaaaaga cgtcctgtgg
    36361 cttccgcgtc gtaattggcc atatgatggt ggtctagact cacttggaaa cgcttggtga
    36421 gcggtcccaa accgtgacgc ttgtattcag gatacaagtt tctagcaaat tctaaggtat
    36481 caatcacagg ctgtgtgatt ttgggcaagt tgtggcgttc ataattggcg ttcataaagc
    36541 ccacgtcaaa actggcgttg tgggcaacca agatactatc tttgcaaaag tcctgaaaag
    36601 cttttaggac ggtaaccaat ggcttggcgc cctgcaaatg cttatcggta atccctgtca
    36661 attcggtggt aaaggcagaa agaggatgcc caggatcaat gaattcatca aactgctcta
    36721 caatatttcc tttaaacatt ttggaagccg caatctgaat caggtcatta ttcatagcag
    36781 ataggcctgt ggtttccacg tcaaagacca cataggtagc ttcgtgcaaa tccatatcaa
    36841 caggatcata agaaataggc accttgtcct caacaatatt ggcttctagg ccaaaaatag
    36901 ccttaattcc agctttgcga gccctatggt agccatgagg aaaactttgc acattagcat
    36961 aatcggtaat agcaaccgcc ttgtgtcccc acttggctgc cgtatcaatc aagctttcta
    37021 ctgtcggtaa ggcatccatg gtagacatat tggtgtgggc atgaaactca acccgttttt
    37081 gcccttctgg catcaggtct ttgcgctcat gatgtacaat ttctttgacc tgctggacat
    37141 tcatggtgag actcttcgta aaaggattgg tctcaatatt cccttgtacc cgtaaccaag
    37201 cccccttagc aatcatatca aatttacgga gctcctcatc gtctttggcc catttttgca
    37261 gagcaaacga ggaggtataa tctgtcattt taaagttgat gatatggcga cctgtcctag
    37321 tcgttttacg ctccacatca aaaaccatcc cctcaaagac aattcggttt tcttcggtct
    37381 caatctctat cattggtgtg atggctgctt tttcaaatcc tgcctgacgc tgcgccacgc
    37441 gctctttata atcaaattta ggagcaggtg ttgcttcttc agccggtggc atcatggcct
    37501 caagggattt ttgggcttcc aaattatcct gcacagcctt ttccacaaga gcctgacgac
    37561 tggaaacaaa atccttggtc aaatgctcag tcatctcctg atctgacacc atatctatgg
    37621 tcaaggtgcc aaagccaaag gcttctaatt gcttgaccag attaggcaga tgattgtttc
    37681 taaaatgatc gttattcaca aaacctggcg ctgcaataat gagtttgtca tcctcataag
    37741 tcactttgag ctttgagaaa gaagatttaa aactagcact attacacggc gcatgctcaa
    37801 aagcttcttg gtaataagct tgaagcagat catctgaata atccacctga gcagcttgga
    37861 tatcaaaggt taccttaatg tcagccgcct caaaagttct tatcaaacga tcatgcaatt
    37921 cacgataagt tgcaatcggt aaaaccgccg caaaggcaaa atgaaattcc cacaagcgtg
    37981 acaccgaatg cacctttacc tcgataatat cagcagatga aaaggcactt gaacgtctca
    38041 tgtcaagtgg catttctatc tggtccatca atttagcgaa taaatctgac ataattttct
    38101 cctgatttta ctaggcttta ttttatcaca attcaagatg attttcgagt tttagatgga
    38161 aagaaaagca gggctttctt ctgacgttct ttggctaatt tattccaagt gatagctcag
    38221 ttgaggtcat ttttttgtca atcttttatc gccaagcgtt tttggtaaag caaaagccac
    38281 tctgtatcag acaagttcac agcaaaaaga ggccattcta aacaggcagc ctcttcattt
    38341 cttttttagt gttcttttgt taaaatttca agggtttcga tgaggttttc tgcattaact
    38401 tcaatgctgt cacctgttgc cttgatttta atttccacga taccttcagc ggctttttta
    38461 ccaacagtga cacgaattgg caaaccaatc aaatcgctat cagagaattt cgaaccgacg
    38521 cgttcattac gatcatctgt caagacatca taccctttag ccatcaagtc agcttctaac
    38581 ttcgccgtca agtcttgggc cacttgatct ttgacattaa ctgtaatcaa atgcacgtca
    38641 aatggggcta attctttagg gaagttaata ccccaagcat aacggtaatc gccttttggt
    38701 gtcttgttca caaagagacg ggcatgctgt tcaatgacag cagacaaaat gcggctaacc
    38761 ccgataccat aacaacccat cacaattgga actgttctgc cattttcatc aagaattgtt
    38821 gctcccatgc tgtctgaata acgagtgccc aacttgaaga tatgaccgac ttcgatacca
    38881 cgcgcaaact ggagaacacc atgaccatcc ggagacattt ccccttcttt gacttcacga
    38941 atatctacat attcagcctg gaagtcacgt cctggattga ctcctgtgac atggaaacca
    39001 tccttgttag ctcctgcaac ggcattggta aggttttgca ctttgcggtc agccacaatg
    39061 cgactacctt gtgccaagtt gacaggccca agtgagccaa aacctgcacc aaagaaagca
    39121 cgggcttctt cttcactagc tggctctaaa aaatcagcag ctagatagtt ttttaattta
    39181 acggtattga tatggtcatt tccgactagc aaagcaacaa caggttcatt atctgccaca
    39241 aaaagcaagg ttttgatggt ttgtgtttca tctactgaaa gaaaagcagc cacttcatca
    39301 atcgttttgc aatgtggtgt ctcaacttct gccaaggcat cttcagcagc tacttttgag
    39361 gaaggtttgt attcgttagt tgccatctca aggttggcag cgtagcttga ttctgttgaa
    39421 taagcaatag tatcttcacc tgaaatcatc caagcagcca attctgcctt aatctcttct
    39481 aagacctctt ttggaatatc atccattgac gtaatagact tgtcaagaac cacccagcga
    39541 tcaaggtccg tacgagctgg tgtaatggcc ataaattctt gggaatcttt cccccccatg
    39601 gcaccgccat ctccaataat ccctttgaaa tctagaccag ctctggtaaa aatagcttcg
    39661 taagcttgac gataatcttc gtaggtcaca tctaaatctt catagttgtg atgaaaacta
    39721 tagccgtctt tcatgataaa ctcacgggta cgaagcaagc cattacgcgg acgtttttca
    39781 tcacgatact tagactgaat ttgataaagg tttaagggca attgcttgta agatttgacc
    39841 gcatcacgca ccaaagtcgt aaaggtttct tcgtgggtcg gacccaagat aaagtctgag
    39901 ttatcacggt ttttaagctt atagaggtcc tctccatagg tctcataacg gcctgattca
    39961 cgccagagat cagctgtcaa aagagctggt gccaacattt caacagcacc gatcttttca
    40021 aactcttcac gcatgatggt cttgaatttc tcaatggtac gatttgccag tggtaaataa
    40081 gcatagatac cagcagaaac ttggcgcaca taaccggcac gcaccataag cgcgtggctg
    40141 ataacctggg catcacttgg catttcgcgc aaggttggga taagcagttt actttgtttc
    40201 atagattatt ccgttttaat acatcccctt atcctcaaac ccttctggaa acatcaagta
    40261 agagtgaagc gggattattc aataatttga atgtgtattc ctttcatttt gagtaagact
    40321 tcaacccttg caaatcttag ggttgaggtc tttttcgatc tttaacatga taaggtcagc
    40381 ttaagatgac ctatcttgat tagaaaaaga cgcgcatgat atcattccat gtcacagcaa
    40441 tcatcaatac gaccatgata gcaaccccag ctagggtgat ataggcctct gtttcttgct
    40501 tgataggctt gcgacgaatg gcttcaatga tattcatcaa gatttttccc ccatcaagtg
    40561 caggaatcgg aatcaggtta aagatcccta aattgatcga aagcattgcc ataagagata
    40621 agactgactc taagccgttt tgagcagcct gattggacat gtcatacatg gcaacaggtc
    40681 cacctaattt attgagacta aagccagtga tcaatccttt caaagcattc aaaatagtaa
    40741 acgccccact ccaagctaat tctagaccgc ctaagagctt atccttaaat cctgttttca
    40801 gactcgcctt aactcctatc gtgtatgtct ttgcatgctt ttgtggtttc acagccaccg
    40861 tttttaagcg ctgatgtgac ttgtaggtga ccttaatggt ctgtgacggg cctagatccc
    40921 gtgttgcaag gtctacagcc tcagtgagat cattccagct agtcacctta taaccattaa
    40981 ttgcgacaat ttggtcatta tctcgaagac cagccttagc tgctgctcca ttttcttgaa
    41041 cacggacatg attgctacta aaatctggca tcccaccttg taaaaagacc aagaggataa
    41101 aaaccacaat ccctaaaata aaattattca tgggacctgc aaaattggtg attaaacgtc
    41161 cgccaatact agcattttga tattgaacat caagcggagc aattcttatc tcagtaccgt
    41221 cttcttcaac aatagtagca tcgtgagcga ccttatatgt cttggtttct tctaaaacca
    41281 agccagtaat actcagctga tcttctaagt catagcctgt aacatgcatt gggagactcg
    41341 ttgggtctaa cttactttga gacaagttga tgcgcttaac aaaaccttgc tcattaagag
    41401 ttaaacttgc tggagtacct gttttgatct cagttttgtc atcaccccaa ccagccattc
    41461 gcacataacc gcccaagggt aacatcctca aggtataaag agttcctcct tggtcaacat
    41521 gagaaaaaat tttagggccc attccaatgg caaattctct tactagaatg cctgattttt
    41581 tagcaaaata gaaatgtcca aattcatgga caatcactaa aataccaaaa ataataataa
    41641 aggttattat tcctaacata gatttccttt cagtctattg cttttttgag ttctgtcgcg
    41701 ttgttgcgac tccaaacatt acctcttaag attagaataa accaaagaga tgcatgatgg
    41761 gaaaaacaaa aatcatggaa tcaaagcggt ctaaaatacc accatgtccc gggataagct
    41821 tacctgaatc tttaacacca aagtgacgtt tgagagcact ttcaaccaaa tcaccaaatt
    41881 gggcaaaaat ggaaaaaaga gcgaccaaaa ccaacatggt caaaaagtga tgtggcgcat
    41941 aaacagacct atcaatcacc ataaagataa aagacacaag gactgcacaa gcgatccctc
    42001 ctaaactacc ttcaatggtt ttgtttggag aaactgtagg caatagtttg cgtctgccaa
    42061 actgacgccc aatcagatat gctccaatat cagtggccca aacgataaat aaggccaaaa
    42121 agaccttgtc aattcctgac aggcgagcat tgatcaaatg ttgaaaacca atcccaacat
    42181 aaaaactagt tgcaatcgga aaggccgcat catcaaatga ataagctctg ctatttaaaa
    42241 cagtacctgc caaaatgaaa aaaaccatta agctgtaaaa agcaacattc gcatcaattg
    42301 gtaaaaaagt gaggtaatgg tccatcggaa ctgccaatac aaaggcggct aacatggcaa
    42361 aaacaccttc aaaagaaaaa acttctaatc ttttcatttt cagtaattct gataccccaa
    42421 tcatggccaa aacgcctaca aataattgga atggtaggtt tcctattatg agaaaaggta
    42481 ggaaaatagc tactgctaat cccccccaaa caacacgttc tttcataact atctcctttt
    42541 agaccttgcc aaaacgacgt tggcggtggt tataatccgc aatggccttt agtagctctg
    42601 cctttttaaa atcaggccac aagactggtg tgaaataaaa ttcactgtaa gctgattgcc
    42661 aaggcaagaa attgctcaaa cgcagctcac cactggtgcg aataatcaaa tcggggtctc
    42721 ggtagagata tggcagatgg tctgtcatca agtaattagc aatcaaatcc tccgtaatat
    42781 ctcctggatt taatttggca tctaagacat cttgggcaat aaagtgaaca gcactggtaa
    42841 tctcagcacg tcctccataa ttgagagcaa aattcaaaat taaacccgta ttacgttttg
    42901 ttttgtcgat ggctgcattt agcgctgcca aggtatcttc aggtaaacga ctagtctctc
    42961 caatcatttg aatctttaca ttgttctcat gaagcacagg cacatatttg tcaaagaagg
    43021 ttactggtaa attcatgata aaagacacct catcttgggg tctggaccag ttttctgtcg
    43081 aaaaggcata gactgtcaga actttaaccc ccaactcaga tgcagtaatc gttacttctt
    43141 gtaaagcatc catcccagct ttatgcccaa acactctagg ttttagtctt tttttggccc
    43201 aacgtccatt accatccatg ataattccaa tatgctttgg gatactcccc aaaacttttt
    43261 ttgttgattt cgcttttaat ccaaacatgt tttttcctta ttgataagca tacttatctc
    43321 atattttatc atatttcacg ccttttggct tgttttttat caattagatc ttatcttcac
    43381 aaaatgttca cctatcctct gatagatgtg tcagcttttc atgaacctgt tagcatcaga
    43441 ataactgttt caatccaggg atcaaaacaa aaaagtttgg catatttcat ccaaaccttc
    43501 cccgccttaa tggctttcaa tggcactgtc agtgtcagtg ctatctactg tctcaacagc
    43561 aggctcatta acaacaatgg tttcttctgt ttcaaccagt gtcgtttctg tggtagcttt
    43621 tgttacaata cgcttaatag ctgatagctc aaatgtcaaa aagacgccat ccacatctaa
    43681 cacaatcttt ttagctgttg tatcgacttc gtcaacaatc gcaaacatgc caccaatcgt
    43741 tacgacttcg tctccttttt caatagcgtt taactggttt tggcgttctt gcgcttgttt
    43801 tttttgttga cgttgcataa accaaatcaa tccaagcatc actacaaaca ttaaaattgt
    43861 tggaaatccc ataatgtctc ctttttattc tataaaagtc atgtcttact atatcaaaaa
    43921 tcaagcggta tagcaagact agacgttacc tgatttcttc tttaaacgag acgctaagcg
    43981 cagtgcttta ctcacgagtc aaaaaagcta agatgtcatc tggtgtcaaa cttgaatcac
    44041 atacagccct ttggtgctga tcgtaactaa ccagcaaagc cggaactgtc actaattggt
    44101 agttctcacg aaaggcagca agctcagcag catcagcagc gttttcgcta tccacaaaat
    44161 agacttcttt ttgattatct gttgccactt gtgccaactt aggagcaaag cggcgacaat
    44221 aaggacagct cgaacgtcct aaaaagacaa tcatgtcttt gccactagca atagctgatg
    44281 tgacttctgc aacactcgac ggtataaaat tcgcaacgat ttcttcaaat gtcatggatg
    44341 tctccttctc ttttggtctc atgatacact aaagtagcaa ttttctcaat cacaaggtct
    44401 tttcacagct cagagctttt atagccgtca aaaagaatcc gcaaacagca atctttttgt
    44461 ctctattaac accaccctaa tctttttggc gcttcatcaa caggctaata gccaataaaa
    44521 gcagagctac tccagctgct aataagcctt ttgaggaggc ttcacccgtt tttgggagct
    44581 tttggtttgt tttagctctt tttggcctag tcattaatga tttatctagg tcttttgagc
    44641 catctggtgt tttggtttga tgattgtctg tctgactctt ttgctgagag ggattagctg
    44701 tttttgagga tactttgagg accagggcag ttaggccatc aatagtcaag ccttctttgg
    44761 taaactggac tcccttaggc ttagcaatgg cagtaacacc agcttgctca gcatcagcaa
    44821 gcacttgggc tcctagcaaa tagcggtagg cttgaggtaa aactaccttg cgggtcttgt
    44881 tgtctgcatt gacaaagaca gcatagcgat ctccatttga tgccaccgtt tggtaaccca
    44941 tgataaggtc ctcttgttga ataccatctt gtcctgcttg ggtgatcaag gtcacatccc
    45001 gatctacctc agctttggtt gcttttgtaa aggcatctgt tgagcgacgc aacgcaatta
    45061 ggccctgtgt ataggctttt gtttggttgc taatcgggtg agctatggaa tctgttgcct
    45121 ttgtccagtc aaaatgattg accgcatcag acgaatcata agaatcgtgg atgaagtaag
    45181 ggtattgcgc tacagcatca atcagagtcg ccttatttgg caccttgtca tcagacacct
    45241 ttttcttgta gtcgggattt agaagctgct tggttcgtcc atattcctga ccagaatgga
    45301 taaaggcagt cccttgagca gttaaaatca tggtatttcc tagacgaata cgcttgtgaa
    45361 tctcttcttc agccacttta ggatccttat tgatggattt ggcaataaca tcatgtaagg
    45421 tcaggttgtc atgggctgca atatactgca ctacatctcc tggggcatct gcttcaaaat
    45481 tactaggctg tgctttgatc gttttgaata aaccttctag attttttgcg ccaccagtaa
    45541 taaaggctgc tgtgccttca tttggaaaac ctgacttgag ggtatttctg atatcatcag
    45601 agaaaacacc gaccgtattg gttgctttca tccaatcttg atctgccgca atttcttttt
    45661 tcccctcatc accttggtag gtacgccagc cttcgccaat cataatggta tttggattaa
    45721 tggctttggc tgccttaaag gcttgctcaa tagctgccgc atcatggtca cccatcatgt
    45781 cgaaacgaaa accatctacc ttgaattcac gagtcagata agcaatcgaa tccaccaaga
    45841 tacgacgact catggcatgt gtcgttccta gacgacctcc gccaaaactc tctctagctg
    45901 tgccatcagc attcataaaa tgatagtagt tgggttccaa atcttcaaag agataggttc
    45961 tagccgtgtg gttataaacc acatcaaaaa taacacccat accacgtttg tgaatctcat
    46021 tgacaaggtt tttaagctct gcgatacgta aagcagggtc gttaggattt gccgaataca
    46081 tgccagaaag ggcaaagtag tgttgtgggt cataacccca gttgtaatta ttgtctgaag
    46141 acgtgtaggc tgttgagcgg ctcttgtcca gctcattggc ataaaaataa ctcaaaaccg
    46201 gtagcaattg aacgtgggta acccccaagt ctttgagata gtctagctgt tcaacgaaag
    46261 ctgaaaaagt cccaaaaggg tgtgttaact tgccttctag agccttatct gacgtaaaat
    46321 ctcgcacatg tgcttcatag ataatagcgt cttcacgctt tttaaagttg ttaattttgg
    46381 caaaatcaag gcctgttggt cctagtttgc ttggatcaat aaaggcagct tttgctgttt
    46441 tgatgtcatc agtagcagtc gcatcattcc aggcagcgag agatttggcg taaggatcca
    46501 aaaccatgac tttttcctga ccgcgcgtga tttcataaag gtaatagtag cctgtgtaat
    46561 cactaatgcc cttgatacta tcagaagtca gatgagctct ccaaacaccc ttgtccgact
    46621 tggtcaaatc agcttgacca accacccttg tctgatcttg tttatcataa acgacaacct
    46681 taacagtatc agcacttgga gaccatagcg ctaaatcaac agaaccatcc ttagctaggg
    46741 tagctccaag ttcaccatcg taagcataga gtttatcttt taattgccag gattggcgag
    46801 cgacttggct aacttctcca aatgtaaccg tgtagactgc accttgcgcc ttaaaatcac
    46861 cctttaatgt cattacagac ttatccctgt caagtgtcaa ctcatcaatt gcaacagttt
    46921 tccctgcttt gtcagtgatc ttgatgtttt gtttcaccgc atcttcatca agcccatcta
    46981 aggtcgtaaa aatggcttta atctcatttg gcgtggtttg ttcagctcct ttgagactaa
    47041 cctgatcaat ataataagga ttgttgtaga cttttgggtc agtgtctttg acaaaaacct
    47101 gagtatgatt gtctaactct ttaaacagat aatcttttgg ttgcacttta atagcatctc
    47161 ctgtcttact tttatcaagg attaaaaatc cgatttcgtt agctccttct tttaagggga
    47221 catcaacata agctccataa ggccctttat gtgacaagtc aagtccattt ggccagtcgg
    47281 tcgttggggt tttgacatct ttaaaggtcc agacagctaa gttatcgtag tgtcccgatt
    47341 gattgtggta gttgattcga aggtaacctt ttttcaaagg tcgataagcg tgcgcatggt
    47401 aattctcgtc tatccaaact tcattcattt ttggtgtgag aagcgagatg tgctggtcct
    47461 ttgaaagatt ctctccagct ttattattaa tgagataaga cacttgctgg cggtgtttag
    47521 ctgctagtgg cacatctaga taatagccat agtcatcttt tttcgctttt gtcatggtga
    47581 tagcaccatt tggccaatcc tttgaaggtt gatccacatc tccccacacc caaagtccca
    47641 agcttcctag cgactcacca gctggcaatg ttttaaaatg catacgcaaa tgatgctcag
    47701 caatactagc aggattagct gacgagctgt ctaaaactgg ttttgcctcc ttgctagacg
    47761 attgaagacc atcaccagat ttagcattgc catctccttg ttgagcttca tcgcgggcta
    47821 gtccaacaat ggttttagca gcaacttgat gtgttaagat actaggggtg caccctaaca
    47881 cgacagtccc agttgcagcg attacaaaac ctatccccca cttttttaac agatattgat
    47941 agcgctttga tccttggttg actttctttt tcataagacc tccatttagt ataacattca
    48001 aacaagacta tcttatcaca ttgcgcaagc gcttgcaaga catgccgcac ttcaacttag
    48061 aacacagctc aaacaatgac tatcacccct taataggatc tcaaaaatca aacagccaaa
    48121 acgagtttcc actcgcttgg ctgttgggta aactcaaatc acatacaact gctaggcctt
    48181 cagtttgaga caaaaggcat cccaaggttg taagtgcttg gtctcgaaca ccgtgtccac
    48241 atctgtattg gtgatgatca cttgtgcacc tgttaagtct ttggcaaaga cttgctcctc
    48301 atcagacaca ttgacaacaa tcacatacgt ctcttctcca agctgacgct gataagcaaa
    48361 cactttatca gctgttggca agaggtgata atccgcttca actagccagt cctgctcctt
    48421 acgcaaggca atcaattttt ggtaggtgta aaaaatagag tctggatcag caagggcatc
    48481 ggccacattg atgtcttggt agttggggtt gaccggcaac caagtctctt ttgcttcaga
    48541 aaagccagca tgtgtgtcct tagaccactg catcggcgtt ctagcattgt ctcgtcctac
    48601 cttacggatg ctatccatca cacgcgcagc tgacactcca tttcccatag cttccttagc
    48661 atagttaagg gactcaatat cattaacctc ggttaagtct ttaaaaggat aattggtcat
    48721 gccaatctct tctccttggt aaatgtaagg ggttcctcgc atcaaatgca gtaaaatagc
    48781 taaagccttg gctgattttt ctcggtaagt gctgtcattg ccccaaatgg ataagacccg
    48841 tggcaagtca tggttattcc aaaaaagcga attccagcct tctcccaact ttaattctgt
    48901 ctgccacttg ctgaaaatag ttttaagagc aggaacatcc agttcttccg cataatccca
    48961 cttgggagcg tcaggtttat gttgaagacc cacatgctca aattggaaga ccatggataa
    49021 ctctttgttt tcagggcgag aatactggcg agcaatttca ggcgttgctc cccaagtctc
    49081 accgacagtc atcacgtcat gattgccaaa ggttgcttga ttcatttctt tgaggtaatc
    49141 gtgtaagcgc ggcccgttac ccgtgatttc agaatctgga accttcccaa tcaggtcaat
    49201 cacgtccata cgaaaaccac cgatgccttt agcaatccag aaattcatca tatcgtaaat
    49261 tttatgacgt aaagcttcat tctcccagtt gagatctggc tgacgtttgc taaaaaggtg
    49321 caaataatat tggcctgatg cattatcata ttcccaagct gagccactaa aaatagaagt
    49381 caaatcgtta ggttcatctc gccaaatata aaaatcacgc tcaggactat tagggttttc
    49441 cctagcttca acaaaccaag catgctcatc agacgtatgg ttgaccacca agtccatgat
    49501 aatcttaatg cctcgctcat tagctgctgc taagagctca tccatatctg ccatgtctcc
    49561 aaaaacatca gcaatcgctt catagtcaga gatgtcataa ccattgtcat ccattggact
    49621 ttggtaaaca ggagataacc aaatggctgt aatgccaagt ttttgcaagt agtccagttg
    49681 actggtaatg ccttttaaat caccaatacc attgccgctg gtgtctttaa aggatctcgg
    49741 gtaaatctga taaatcgtag ctttatgcca ccagtgtttc ttcattattc ttctcctatt
    49801 aatcattcat tcatctttta ttttaacgtt ttttctcttt ttgtgcaatc gtttgcgcac
    49861 atgataaaaa ggttggaacc caaccttttt atcaatatcg cttacacctt aacgaatagc
    49921 ttgttcggta tctctgtcaa agaaatgccc tttagccaca ttaaaggtca agctaacttg
    49981 ctcccctgga ctatggaaat cacgcgcatc tacacgagaa gcaaactcag tttgtccaag
    50041 cttgacatag agcatcgtct cagagcccag taactcagaa accaaaactt ctgctgtgac
    50101 agttgcactt ggataagtat catgcacgat ttgtctacta gaaatgtctt ctggacgaat
    50161 accaaaagtg acttttttcc ccttgtaacc agccgcttca agcatttttg cttgaccttc
    50221 aggaatcgca atagcaagac catcttcact cacaatgcgg ccatctttaa cttccacttc
    50281 aaagaagttc atggcagggc ttccgataaa gccagcaacg aatttattag ctggaaggtt
    50341 gtacaattct tgtggcgagc ccacttgctc aattttacca atcgttccat taccttgagg
    50401 gtttttagtg gcactcatga taacgatacg gtctgccaaa gtcatggctt ctgtctggtc
    50461 atgggtaacg taaatagtcg ttgaaccaat cctacgatga atctttgcga tttcagcacg
    50521 catggagaca cgaagcttcg catccaagtt tgacaaaggc tcgtccatca agaatacctt
    50581 agcatcacgg acaatagcac gccccatagc cacacgctga cgttgaccac cagacaggtc
    50641 agcaggttta cgctctaaaa attcggtcaa accaagaatc tgcgcagctt ccttgacacg
    50701 acggtcaata tcatcttttt tgtacttacg taatttcaaa ccaaaggcca tgttatcata
    50761 tacggtcatg tgtgggtaaa gggcgtagtt ttgaaatacc atggcaatat cacggtcttt
    50821 aggagacttg tcattcacca cttcgccacc gatttttaac tctccttcag aaatgtcctc
    50881 aagacctgca atcatacgaa gagtagtcga tttaccacat cctgaaggtc caacaaagac
    50941 gataaattct ttgtctttaa tgtctaagtc aaaatcttca acggcataat gagtagtatt
    51001 tgggtatttt ttatagatgt gatttagatt taattcaacc atgtgtaact cctatttgaa
    51061 cagcaaacgt tctagctgac tgcaaagcta gaaaccgctg atatctttta tatgatgctt
    51121 ttattataac aaaaatagcg ctttcaaaac agagacaaag cgaccaaact tctttgggca
    51181 acttgcacaa tagcagggct agtcaagtcg taagggacca taaaccaaga aaactatgta
    51241 aacacaggcc ggtcacagga gccaaaacaa gctgaacgat ctatcattct ccttatcagc
    51301 ttcagtcaag gcatcaaaaa agaaagccct gtggctttcc taatatttca acaaaaagag
    51361 atgagcaaaa gctaaatcat ccaattgctt caagtggagt cctgattgct gagcaaattt
    51421 atcaagtttg tattgcaagg agttacggtg aatatacaag cgctgcgctg tctgcaccag
    51481 attgccgtgt tctgaccata gcgcgtgaac cacatcggcc atatcacttt gaggacttag
    51541 gaattggtga aactgacgcg tcaatgccaa aaaagtgtgg tgcgacagca aagaccacag
    51601 catcagtcct gaaaatgtta ataattttct gcctgattgt tgccttaaat aggccgttaa
    51661 cagctgacat tcctcctcaa aacactctct caatgtaccc gcagctactt gataccaggc
    51721 atttccaaca aacatggtta aggccaaacc aaaatcactt tctagcgttg gcaagagatc
    51781 ttcaagccag cgcaagacct ttattgagat tgcttgtcgg cataaaaatg ccgtttgggt
    51841 tgcactgata ggcaaaatga cctcaagccc tgcaatcatc tgttggagca actcaactaa
    51901 gtcatctggt aaagcctgct gatgattgag gtaaatgaac tgagaagtct catacagctg
    51961 agggggattg ccctgatgat caagcaaata ccgctgccaa ggtgacccta gcggctgcaa
    52021 gacctcacac tgacccagcc ctacctcaag taataagcgc tctttttcgg ataaacatga
    52081 tttgggaaaa tgcacatatt ggtccccttc tttgacactc acccactctt tatctcctaa
    52141 cggatgaggg cctacttgca tctctggaaa atagtctttt agctccatag ccattccttt
    52201 catgacggtc tttaaaccat tataacacat gactctttat cacacagttc ggtttgttgt
    52261 cagcacgatt ttgtattttc tgactttcta attattaaag ctaaataatg gttattcatt
    52321 tttagcaaga acattcaatc aaatagctat ttatcggaag attaatttat gttt
//
LOCUS       DAUCWU010000006        52159 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUCWU010000006 DAUCWU010000000
VERSION     DAUCWU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52159
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.006"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..72
                     /locus_tag="VGY24_000332"
     CDS             <1..72
                     /locus_tag="VGY24_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4283346.1"
                     /translation="NNKRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(372..1244)
                     /locus_tag="VGY24_000333"
     CDS             complement(372..1244)
                     /locus_tag="VGY24_000333"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ4283347.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1468..2778
                     /locus_tag="VGY24_000334"
     CDS             1468..2778
                     /locus_tag="VGY24_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ4283348.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            2856..4012
                     /locus_tag="VGY24_000335"
     CDS             join(2856..3108,3108..4012)
                     /locus_tag="VGY24_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4283349.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQPVKMVSSTLKIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHTQFEIKKLEKEHKLLQEELALPKKVPGLLEAKLEIKFQF
                     LKENSVSLNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYG
                     SVRITQELCRRGIHVNYKRVGRLLHQLGLYAKGSRYQYKYYNRRRSSLTRPNLVNQCF
                     QATGKNKLWLGDLTYIPTQEGILYVSVFIDVYSRKIVGWAMGRRMQDKLVTEAFNQAY
                     NREKPKEGGIVHTDQGSQYTGARFQDLLRQKKCKSSMSRKGNPYDNALMEAFYKTLKK
                     ELVNDAHFATIEQAQLEIFKYSETYYNPKRLPSALGYLSPVEFEKNSYSLA"
     gene            4133..4288
                     /locus_tag="VGY24_000336"
                     /pseudo
     CDS             4133..4288
                     /locus_tag="VGY24_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4335..4865)
                     /locus_tag="VGY24_000337"
     CDS             complement(4335..4865)
                     /locus_tag="VGY24_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ4283350.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(4858..6192)
                     /locus_tag="VGY24_000338"
     CDS             complement(4858..6192)
                     /locus_tag="VGY24_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEQ4283351.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
     gene            complement(6414..6713)
                     /locus_tag="VGY24_000339"
     CDS             complement(6414..6713)
                     /locus_tag="VGY24_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283352.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     VNFDDLRLTMVDMVSLSSEYDVIVTDMILEQTTDSEILFFNQMAPSVVANRLTDM"
     gene            complement(6869..7336)
                     /gene="perR"
                     /locus_tag="VGY24_000340"
     CDS             complement(6869..7336)
                     /gene="perR"
                     /locus_tag="VGY24_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ4283353.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQPDF"
     gene            complement(7388..7843)
                     /locus_tag="VGY24_000341"
     CDS             complement(7388..7843)
                     /locus_tag="VGY24_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ4283354.1"
                     /translation="MIYSFQNPSEDVLKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            complement(8031..10673)
                     /gene="polA"
                     /locus_tag="VGY24_000342"
     CDS             complement(8031..10673)
                     /gene="polA"
                     /locus_tag="VGY24_000342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ4283355.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPVRLEQGRLIRKAFTPSQEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKGLQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(10804..12531)
                     /locus_tag="VGY24_000343"
     CDS             complement(10804..12531)
                     /locus_tag="VGY24_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ4283356.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAQKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     QSKVSSWTK"
     gene            complement(12547..13743)
                     /locus_tag="VGY24_000344"
     CDS             complement(12547..13743)
                     /locus_tag="VGY24_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ4283357.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(14230..15321)
                     /gene="ulaG"
                     /locus_tag="VGY24_000345"
     CDS             complement(14230..15321)
                     /gene="ulaG"
                     /locus_tag="VGY24_000345"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ4283358.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(15574..17220)
                     /locus_tag="VGY24_000346"
     CDS             complement(15574..17220)
                     /locus_tag="VGY24_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ4283359.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(17545..18249)
                     /locus_tag="VGY24_000347"
     CDS             complement(17545..18249)
                     /locus_tag="VGY24_000347"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ4283360.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNSSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVILEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(18251..19114)
                     /locus_tag="VGY24_000348"
     CDS             complement(18251..19114)
                     /locus_tag="VGY24_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ4283361.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDSATEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVILSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETRAAIKEAQDF
                     LYPLIEKAGLT"
     gene            complement(19119..19781)
                     /locus_tag="VGY24_000349"
     CDS             complement(19119..19781)
                     /locus_tag="VGY24_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ4283362.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEVRGDRGEIQIELYGDWSYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(19872..20357)
                     /locus_tag="VGY24_000350"
     CDS             complement(19872..20357)
                     /locus_tag="VGY24_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4283363.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            complement(20480..20758)
                     /locus_tag="VGY24_000351"
     CDS             complement(20480..20758)
                     /locus_tag="VGY24_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4283364.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(20829..22262)
                     /locus_tag="VGY24_000352"
     CDS             complement(20829..22262)
                     /locus_tag="VGY24_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4283365.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTSGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(22569..25070)
                     /locus_tag="VGY24_000353"
     CDS             complement(22569..25070)
                     /locus_tag="VGY24_000353"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ4283366.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKNFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(25281..26420)
                     /locus_tag="VGY24_000354"
     CDS             complement(25281..26420)
                     /locus_tag="VGY24_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ4283367.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            26904..27176
                     /locus_tag="VGY24_000355"
     CDS             26904..27176
                     /locus_tag="VGY24_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283368.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHQTVHAAERCTHVSDNPNVF
                     LCSDDGKPTIQTNSVPNPTQNQKSGPLDWIWNTIKGWWEQVKSWFS"
     gene            27364..27531
                     /locus_tag="VGY24_000356"
     CDS             27364..27531
                     /locus_tag="VGY24_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283369.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHN
                     GPDSWLLYTVW"
     gene            27941..28174
                     /locus_tag="VGY24_000357"
     CDS             27941..28174
                     /locus_tag="VGY24_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283370.1"
                     /translation="MKKKLSLFMIATAAVVSLSLATPENQTVSANEAVATQACNYDFY
                     GYCGPISWLFYKTKHLLMGEQPTTTTSTTTVTK"
     gene            complement(28403..28819)
                     /locus_tag="VGY24_000358"
     CDS             complement(28403..28819)
                     /locus_tag="VGY24_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283371.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGEQKDSWELGYN
                     QGLTKGKEAGQKATWTDLTPTVPEVSELPSDIEEANKGTYKEGYTDGYKEGYNESWKS
                     QHPVLTPVKVIWDLISYWLQRFFPNNQATTTAQSMS"
     gene            complement(29074..30789)
                     /gene="slo"
                     /locus_tag="VGY24_000359"
     CDS             complement(29074..30789)
                     /gene="slo"
                     /locus_tag="VGY24_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ4283372.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTSEQPKPESSELTIEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGEKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKDLQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHRGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(30813..31298)
                     /locus_tag="VGY24_000360"
     CDS             complement(30813..31298)
                     /locus_tag="VGY24_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ4283373.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSIVANMTKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(31303..32646)
                     /gene="nga"
                     /locus_tag="VGY24_000361"
     CDS             complement(31303..32646)
                     /gene="nga"
                     /locus_tag="VGY24_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ4283374.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     HIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFHKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNIERDIFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAIQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDVMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRK"
     gene            complement(33017..33556)
                     /gene="nusG"
                     /locus_tag="VGY24_000362"
     CDS             complement(33017..33556)
                     /gene="nusG"
                     /locus_tag="VGY24_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ4283375.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(33729..34772)
                     /locus_tag="VGY24_000363"
     CDS             complement(33729..34772)
                     /locus_tag="VGY24_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ4283376.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            complement(35099..36391)
                     /locus_tag="VGY24_000364"
     CDS             complement(35099..36391)
                     /locus_tag="VGY24_000364"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ4283377.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGERIDHYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            36830..37708
                     /locus_tag="VGY24_000365"
     CDS             36830..37708
                     /locus_tag="VGY24_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283378.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGAGYAIFRVRSHQKRLAKAKVAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            37721..38983
                     /locus_tag="VGY24_000366"
     CDS             37721..38983
                     /locus_tag="VGY24_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ4283379.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIVNANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(39103..39729)
                     /locus_tag="VGY24_000367"
     CDS             complement(39103..39729)
                     /locus_tag="VGY24_000367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ4283380.1"
                     /translation="MARLNVKPTRMEFSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNMAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(39774..41189)
                     /locus_tag="VGY24_000368"
     CDS             complement(39774..41189)
                     /locus_tag="VGY24_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ4283381.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTRVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(41190..42965)
                     /locus_tag="VGY24_000369"
     CDS             complement(41190..42965)
                     /locus_tag="VGY24_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ4283382.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKKEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     ESNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(43166..43486)
                     /locus_tag="VGY24_000370"
     CDS             complement(43166..43486)
                     /locus_tag="VGY24_000370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ4283383.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(43483..44481)
                     /locus_tag="VGY24_000371"
     CDS             complement(43483..44481)
                     /locus_tag="VGY24_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ4283384.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLNHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(44497..45081)
                     /locus_tag="VGY24_000372"
     CDS             complement(44497..45081)
                     /locus_tag="VGY24_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ4283385.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(45149..45628)
                     /locus_tag="VGY24_000373"
     CDS             complement(45149..45628)
                     /locus_tag="VGY24_000373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ4283386.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(45630..47630)
                     /locus_tag="VGY24_000374"
     CDS             complement(45630..47630)
                     /locus_tag="VGY24_000374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ4283387.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPSIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLAARVGTIPSTANDHFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLEKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTKYLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLYAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(47620..47940)
                     /locus_tag="VGY24_000375"
     CDS             complement(47620..47940)
                     /locus_tag="VGY24_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283388.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(48395..49414)
                     /locus_tag="VGY24_000376"
     CDS             complement(48395..49414)
                     /locus_tag="VGY24_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ4283389.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(49451..49816)
                     /locus_tag="VGY24_000377"
     CDS             complement(49451..49816)
                     /locus_tag="VGY24_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ4283390.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            49990..50670
                     /locus_tag="VGY24_000378"
     CDS             49990..50670
                     /locus_tag="VGY24_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4283391.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(50792..51439)
                     /locus_tag="VGY24_000379"
     CDS             complement(50792..51439)
                     /locus_tag="VGY24_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ4283392.1"
                     /translation="MAVALSKEVIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(51442..51570)
                     /locus_tag="VGY24_000380"
                     /pseudo
     CDS             complement(51442..51570)
                     /locus_tag="VGY24_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid dehydrogenase"
     gene            complement(51565..51951)
                     /locus_tag="VGY24_000381"
                     /pseudo
     CDS             complement(51565..51951)
                     /locus_tag="VGY24_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
BASE COUNT        15648 a        11268 c         9407 g        15836 t
ORIGIN      
        1 aacaacaaac gaattaaggc aaaactaaaa ggatttagtc ctgtccaata cagaactaaa
       61 tcctttcaat aattatttgt ccaacttttt ggggtcagta cactcctagt taacgacttt
      121 gattgatttt attatgatga atcgtgattt ctgaaaacaa aattttcttc atggatttgg
      181 cgtggaactt ttacaataaa aacactaacc tctccctatc gttttttcag gtgaatgctt
      241 ggaattggta taataagcgg agacgaatca ggtgaaaaca gggaaagtat ttgaagaggc
      301 gtttaaacta tctgacataa aactcgttct agaggaaaga cattctttaa aaagttagtt
      361 tattttgcaa cttagacatc aaggtaggtt aaacccttca tggaaaaatc gagactctta
      421 gcactatggg ttaaactacc actggagaca taatcaatcg ctaaaccacg aaaacggcta
      481 atagtggtca tatcaatatt tccagaacat tcaatccgag aacgtcctgc aattagagta
      541 atggcctgtt caatctgttc caatgacata ttatccaaca tgataatatc aacacccgcc
      601 gccgcagctt cttcggcagc agcaaggctt tccacttcca cctcgaccat tttcacaaaa
      661 ggggcatagg cacgcgcttg agcaatcgcc ttttgaacac tacctactgc cgcaatgtga
      721 ttgtctttta gcatgatagc atctgataaa ttaaagcgat gattatagcc accgccaact
      781 ctcacggcat atttctcaaa aagacgtaaa ttaggagtgg tttttcgagt atcaaatacc
      841 ttaatgcgat catcgcctaa ggcttctaca taagcagctg tcatcgaagc aatccctgat
      901 aaatgttgta aaaaattcaa ggcaacgcgt tcacatgtta agaggcttct caccgagcct
      961 atgatttcta aaaccaaatc gccactagtc aaacgatccc catccttaaa ttgatgagga
     1021 ttctggaagg tcacctcggc atcaaatagg gtaaaaaccc tttgaaaaac ggttagcccc
     1081 gctaaaacac cagcttcctt ggcaaaaagc gacaccttgg cttggccatg atgatcaaaa
     1141 atggcattgg tactgtaatc ttcggaatga acatcttctc gcaaggctgc tttcaatgta
     1201 tcatctattt gaaaaggggt taaatcagtt gaaatgattg acatcactaa gtcctctatt
     1261 tttaatatca cttgtaagca ctagaggcaa ttgctattaa cttccgtagg aaatcacaaa
     1321 cctgaatacc tcattttaaa tcccaacaag tgtcaagaca attgtaattt ttttgtgtat
     1381 aatagtcaag taccaatagg aaacaggtgg tcttttgagc gccaaaattg ccaacctatt
     1441 ttcatattct aactaaagga caattcaatg attcatttaa taataattag cgccattgct
     1501 ctagccattg gaattggtta ccgcaccaaa atcaatattg gcctgctggc tattgctttt
     1561 tcttacctca tcgcaaccac tctcatggga ttaagcccca aagaacttct tcatttttgg
     1621 ccaacctcac tcttttttac catttttagt gtctctctct tttataacgt tgcaacaact
     1681 aacggcactc ttgatgtttt ggctcaacac attctctacc gcacacgcac ccaccctaac
     1741 gccctctaca tgattttata cctgatagca acccttttgt ctgctttagg tgctggattt
     1801 ttcactacta tggctgtttg ctgtcctcta gcgattaccc tctgtcaaaa agcggacaaa
     1861 caccctttga ttggagctca agccgtcaat tggggagctt caggaggggc taatttgata
     1921 accagtggct caggcattgt ctttcaaggc ctgtttaagc aaatgggatg ggaagagcaa
     1981 gctttttcac ttggcaatca tatctttatc gtcagcatta tctacccact catcgtcttg
     2041 cttttattat cttgttacat ccgctacagt aagggaagaa caaactcatc cttgactatt
     2101 gaccaaccac cagtcctcag caaggtccaa agacaaacca ccttgctcat gatcagcagc
     2161 atggttttgg tttggctttt tcccttgttg catctcattt tcccaaacat cgcatggata
     2221 gctacttatc gacaaacatt tgatattggc tttgtctcaa tactcatggt ctgcctcgcc
     2281 ttaaggctca aattagggaa gcaagaagcc attttggcta aggttccctg ggctataatt
     2341 attatgctct gcggcatgag cttactaatg tctctagcgg tcaagtcagg tttagtcaca
     2401 ctgatagggc acctcataac aacaaccatt cctcatttct ggttacctct gttcttttgt
     2461 gtgatagccg gagtgatgtc actctttagt tccaccctat ccgttgtagc cccaactctc
     2521 tttcccatca ttgctaccat cagtgctcaa agtcctcaca ttgatattcg cttactcaca
     2581 acagccacta ttattggcgc tctatctact aatatctctc ctttttcatc agctggttcc
     2641 ctcattcaac tctcccttcc ccatatagag gaacgcagtc ttgctttcaa aaaacaaatt
     2701 cttctgggtg tgcccatcag cttaagtttg gcactcttga ccatctggat tcttatgctg
     2761 ctagcttcct taagttagat ttattgtcta acatttggtt ggacttggtg taataaagta
     2821 gacacaaaat taagtggaaa cgaggaaaag tcattatgcc aagaaaaacc tttgataaag
     2881 ccttcaaact ctctgctgta aaactcatcc ttgaggaaga gcagcctgta aaaatggtta
     2941 gttcaacttt aaaaattcat cccaatagtc tttatcgatg gattcaagaa tatgaaaaat
     3001 atggagaaag tgcgttccca ggacatggga gcgcacttcg tcatactcaa tttgagataa
     3061 aaaaacttga aaaagagcac aaactgctac aggaggaatt agctcttcta aaaaagttcc
     3121 aggtcttctt gaagccaaac tggaaataaa atttcagttt ctgaaggaaa atagtgttag
     3181 cttgaatagt catcacgcct gtcaaactct tcaagtctcc cgttcaggtt tctatgctta
     3241 tttaaaacgt cgtccctctt caaggcatgt tgagaatgaa gctttgaagg aaatgattaa
     3301 agccattttc tatgaacata aagagcgtta cggaagtgta cgaattaccc aagaactttg
     3361 tagacgcggc atccatgtca attataaacg tgttggtaga ctccttcatc agctaggtct
     3421 ctatgctaaa ggaagtcgat atcaatataa atactataac cgtcgacgtt cttcattaac
     3481 tcgtcccaat cttgttaatc aatgtttcca agcgactggt aagaataaat tatggctagg
     3541 agacttaacc tacattccta cacaagaagg aatactttat gtgtctgtct ttatagacgt
     3601 ttatagtaga aagattgttg gttgggcaat gggccgacgt atgcaagaca agttggtaac
     3661 agaggctttc aatcaagctt ataatagaga aaagcctaaa gaagggggaa tcgtccatac
     3721 agatcagggt tctcaatata ccggagcacg atttcaggac ctacttagac agaaaaaatg
     3781 taagtccagt atgagtcgaa aaggtaatcc atacgataat gcactcatgg aggcctttta
     3841 taaaacatta aaaaaagagc ttgtcaatga tgctcatttc gcaactattg agcaagctca
     3901 gcttgagatt tttaaatata gtgagactta ctataacccc aaacgtctgc cttcagcttt
     3961 agggtatctc tcacctgttg aatttgaaaa aaatagttac tcattagctt aactttatgt
     4021 ctatttttct tgacaagtcc agggtatgaa gtccccttat acacatactt tcaaaacagc
     4081 tctcaatcct ctgtccgcta caaaaatacg tcaccgctgc cctccagctg cctcattcaa
     4141 cgacaaactt gaagccacaa acagactcat caagggcatt aagtacaatg cctttagatt
     4201 tcagaacttt gacaacttta aaaccaaaat cctcatcgct ttgaacatcc aaaaagagag
     4261 aaccaatctg attctctctc gttgttaatg ataagtcacc cactacagtt gacaatgagt
     4321 ctcttacttc ctgtttactc atttaatagc tcttctggtt cccaagattg attaaactca
     4381 tggtccttaa aaagagaagc caatcctgtt atttgtttca gtcttaaaat ttcatcttta
     4441 tccattccta aatgttttgc aatccagtta tcacttcgcc cacactcatg aagatccttt
     4501 acaatctgac tcatcaaatc aacatcatga cttcctctag ctctattatg tcgaatggta
     4561 gaagccattc gatagtctaa tgacttatca ataacagaaa caggtaatct cccactttcc
     4621 ctttcataaa tatctgaata atctagcatt atacgatatc tatgaaaacc atcaacaata
     4681 ctataccgat cctcttcctt atcgtaatag caaacaatcg gcatagtata accatcagat
     4741 tttatactat catacaataa tttcatttca ggcggagcaa ctgaattcgg attataatca
     4801 tttggagaaa tcttttcaat tggaattgat ttaatctcat agactggact aataaaatta
     4861 ctcactttct aactccttta aactcgtctt atatttctct tttaaaaagg ctactatttt
     4921 ttgttgctct cttgtcaaac caaaccccat agttcgacaa atatgatcgt tttttaaaat
     4981 acagaaacac attcgtttcc agcttggtat atcctttgtt gttacaatat cgtctgtatc
     5041 atcaggaatt tgatctaaaa aaataaccct tgaatgttta aaagaggtat aatttgaaat
     5101 accatttcta gcaatacgat atcctttttc aattagttca ttaattgttt cctccgcaag
     5161 tcctccgcct gttttatgcc aaaagtctat tgatttatta aattttctaa cataattatt
     5221 tcttaacctc actggtagcg tcgataaaag aaactgagta tacgacttcc acgaatgacc
     5281 ctttggtaaa gcaattgatt tatatcccat tgctttagta cgtccataaa tattcgaaaa
     5341 gttaactcct tgaacacgtc ccaaaagctt tacccaagtt tcttgatcta ttattctata
     5401 aagatttagg ctatcaacag catagtcttg aaaaggagat gcaactctca tttgtgatgg
     5461 ttttaaaccc gctttataaa atagatcata aagttcgtta taagaataag aaaacttata
     5521 gtaagcatgc catatgtctt ccactgacca atcatataag ggagatgctg tccagacatc
     5581 tttgaattgt ttggtaatcc agcattcctt ttgataacca tactttttat tgataaaacc
     5641 actatatctg tgtaaggatt ctgaagcacg gtttcctaat atacaaactg ttttttgatt
     5701 tccgtgaatt tgtttatacc acctaccaaa ttgtttcgct aaatcctcct gattcatttt
     5761 ataacgataa gtagtaattg aattattttc caaattaata acataatctt gagatggcat
     5821 tggtcttacc cagatatctt ctgttttgtc atcccacgga taccaaaaca tttcataact
     5881 actaagtgca gttctagtag ccatcggtaa gcacacccaa tacaaagaaa ctttttttct
     5941 accttctaaa gaagtgaatg tttcttgaac atattttgtt gttaaactgt actgtgcttc
     6001 aaaatcttga tgaaacacac ctatttctct atccggataa tatttatctc taaaatccag
     6061 taaaatatta agaagtaacc cactatcttt tccacctgag aaggaaatat agacaatagg
     6121 aaattcatta aacaaaaaat gtaatctttc tatcaatgca tcataaacat tttgatttaa
     6181 atactttttc actaaacttc ctcattatgc atttaaaaat attaatacat agattcacta
     6241 agaatctctt tttcgagctt tttgtttttt aatttttcaa gttgaataat taaaccttgc
     6301 ttttcttttt caaggtaata gactctactc cttgtaagtc taacttattc ttaatcttat
     6361 ttatcgctca ttttttaaat tccgatgctc tcacagatat tatcattgtt tagttacatg
     6421 tctgtcaatc tattcgccac cacactaggg gccatctgat tgaaaaataa aatttcggag
     6481 tccgttgttt gctcaagaat catatctgtc acaattacgt catactctga agacagtgac
     6541 accatatcaa ccatggtcag cctcaagtca tcaaagttga caatctcaaa aaatcctcca
     6601 aaatactctt ttaagaagtt gccaacattg ttataacttc tctcaaccac taatagtttc
     6661 aagcgacgac gcgattgatt tgtaagaaag agattttccc aggtgaggaa caaacaatag
     6721 aggagatgat tgatggttct ttcctttaat tccttatgat ccccagcaaa taactcttta
     6781 acgtaagcaa caaaggtctg gtagtgaact gggggttttt gatttgtaaa ggcactatat
     6841 ttataatgcc tttatttaag ggttaactct aaaaatcagg ttgatctttt gcttggcaat
     6901 ccggacaaat cccgtaagca ataactggga tacgcgtgac cttataacct gtttgctcat
     6961 gggcttcttt ggcaatatcc ataacatcaa catccataaa gtcagcaatc ttcccacata
     7021 tttcacaaac cacattgacg tgttgatggc ccataaagtc atagtaggtg gttaaatcat
     7081 tgctgatttt caattctgag acaaaacctt catcaactaa aactttcaaa ttattataga
     7141 ctgtggcaag gctcatgtta ggaaagttag gctgtaagtc acggtaaatc ttatcggcac
     7201 tagggtgttc tgtggactga atcatgtaag aaataatggc tttacgtgtc tctgtaattc
     7261 gaatatgttt ttcccgtaga tgctctagga cattttcata ggcatctagg gcttgctgat
     7321 gtgaatgaat gtccataagc tgctactcct atcctttttt cctttattat cacgcacgtc
     7381 tctagtttca atgatttgtg atgcattctg gtttagcgac taattgcaaa tagtctattt
     7441 ttaggcagcg attcatcaca atagcctctt tgcctgccga ccgtaaaatg gtttgtgctt
     7501 cttggttttc aaggcctaat tgcgcccaaa agactttggc ctgacctgct aaaaagtctc
     7561 tggcaacttc tggtaaaaac tcacttctgc gaaagacatc tacaatgtca acctcaaagg
     7621 gaattgcctt gatactggca tagaccttct ctcccaaaat aagctgacct gctaatttgg
     7681 gattgactgg aatgatccga taatccatag cctgcataaa tttggcgaca ccataagctg
     7741 ctgtgtcttt gcggtcagaa agaccaacaa ccgcaatggt tttggctgac tccaagtaag
     7801 cttttaggac atcctctgag ggattttgaa acgaataaat cacttgttcc cctatctatt
     7861 ttaataacct ctacagctgt tcaatatagc aggataactc ctaaagcatg gcttcaatca
     7921 cttttaacct tatccgtcaa aaagctcaaa gcagcttcat gacttctcct acaatttttt
     7981 agtttattat aacataagtt gtcaaaatga gaaataacaa gtaagaaaac ttatttggct
     8041 tcgtaccagc tctgcccagt actttcatcg acacataaag gaacagctaa atcaacagca
     8101 gcttccatgg tgtctttgac aagttttttt atagcagtca actcatcatt tggcacttcc
     8161 aaaacgattt catcatgcac ttgaagcagc attttagctc taaaaccacc tgcttgcaag
     8221 cccttatcca gattaatcat agcgattttg agaatatcag ctgcgcttcc ttgaatgggc
     8281 gagttgatgg ctgtacgctc cgcaaaagag cgaacgttga aattacgaga gttgatatcc
     8341 ggtaattcac gacgacgctt aaagagggtc tctacatagc ccttgtcttt agcctcacgc
     8401 accacatttt ccatataggc tttaatccca ggatagcgtt caaaataggt atcaatataa
     8461 gacttggcct gtttacgggt gataccaagg ttatttgaca aaccaaaatc agaaatccca
     8521 tacacaattc caaagttaac agccttggca ttacggcggt cattggcagt aacatctgct
     8581 gccttgtcaa tcccaaacac acgcatagcc gttgaggtat gaatgtctgc cccttcatta
     8641 aaggcagcaa tcaaatgctc atcccctgaa atatgtgcta gcacgcgcaa ttcaatctgt
     8701 gagtaatctg aactcaacaa aacggcatct tcttgagatg gggtaaaggc cttacgaatc
     8761 aaacgcccct gttccaaacg gacaggaatg ttttggaggt tagggtcaac actagacaaa
     8821 cgacctgtct gggtgagatc ctgcacatag cgggtatgaa tcttgccatc agccagaata
     8881 taatcttgta aaccaatcac ataagtagac tgtaatttgg taatctggcg gtaatccaaa
     8941 attttagcca caattggagc aatcggcgct agacgttcca aaacatccac tgctgtagaa
     9001 taacctgttt tggtcttctt agtcatctca agaggtagtt gcatcttctc aaacaagata
     9061 ctgcccagct gtttaggcga attaatgtta aattcttggc cagccatatc ataaatctct
     9121 tgggtgagcg cttcaataat gaccttgttt tgctctgcca tgtcttgcag agtcgcccta
     9181 ttaaccttaa tcccttcaat ctccatttta gcaaggacat tagccaaagg caattcaata
     9241 ttctgataga ggtctagttg ctcatgggca gtgagcttgt ctagcatttg agacttgcta
     9301 tctaataata ctttgacctt acgagctaag tgttctagta agacgtcttt atcaggaaca
     9361 gcccgtttag cacctttacc atagactgta tcgtcttctt ccaaagagat gtcagtaaaa
     9421 atgcgggcaa tggtgctaag ctcattgtct tctacagttg acagaaggta attagctaat
     9481 ctagcatcat agctaggtgc taccaactca attcctaagt ggctcaacaa gaccttgcta
     9541 cgtttaaaat cataagtggc aatgggtttt tggaagacct gcttaaaact atccgtcgct
     9601 aataggctaa ggtcagtgga tgcgtaaatc tgctcaccat gtccccaagc aaaaccaatg
     9661 atggcctcac gatggtaatt atcccgaagc gtttcaaagt agaagatgtc ttctgcgcta
     9721 aacaaatcag cagacacatt ggtcacttct tgataggaaa tggcagctac tggctcttga
     9781 ggcatctgac tcgccaaacc ttttttcagt tggacaaaat ccatctcatc gtaaaattga
     9841 gagaggctag caacatctgg accattgtag acaatatcat ctaagccaat tgtgatagga
     9901 gatgctgtat tgatagtggc gagcgtttta gataggaaag cttggtcacg gtcattgatc
     9961 aagttttctt tcatttttga ggccttgaat ccatcaatat gctcgtaaat gccttccaaa
    10021 ctaccaaatt catgaaggag cttaaggcct gttttttctc caatttttgt cactccagga
    10081 atgttatccg atttatctcc catcaaggct ttgaggtcaa taaactggtt tggcgtcaag
    10141 cccatttttt ccatgaggta agccggtgtg aattcttcaa attcggcaac ccctttttta
    10201 gaaatttcaa ccacggtatt ttcgtcagtt aactggatca aatctttatc accactgaca
    10261 atggtcacat caaaaggcac ttctgtgcgc tccgccattt tatcaagcgt cccgataata
    10321 tcgtctgctt cgtagtgttc caactcataa taagcaatcc caagggctgt caacatctca
    10381 cgaatataag gaaactgctc acgaaattcc tctggtgtct tggcgcgacc cgccttataa
    10441 tcggcataca tttctgttcg gaaggtggtt ttgcccgcat caaaagccac cagcacgtga
    10501 gtaggctgga ctcttttcat catgtggtct agcatcaaat gaaagccata aatggcattg
    10561 gtatgaagac cgctatgatt tttaaagcgg tcaatttgat tgtagagggc aaaaaaggca
    10621 cgaaaggcaa cagatgagcc atcaattaat agtaatttat tcttgttttc catagcctta
    10681 ttataacaca gagggacggg cttttctcct ttaagccatt ggttttgctg ataagtaaat
    10741 agttaaccta aaaagcctag gaaaattcct agacttctct atttgctatt tagacaaaag
    10801 cacttatttt gtccaacttg atactttaga ttggtttgcc tctacccatt ttttggcggc
    10861 agcttctggg ctcatgcctt tgttgatgtc aagcatgaca gcttccatat ctttttgggt
    10921 ccagtggaac ttatcaatga ttttgtagac atttggaaga tcttttttca agcctttacg
    10981 agcaatggta ttgatgttct cagtcgagcc aaaggtttct ttaggatctt taaggtactt
    11041 caagtcatac ttggcaaaca tccagtgtgg tgaccaagct gtcacgacaa taggatcttt
    11101 tttcttgatc gcttgatcca aggaagtcgt catggctcct gttgatgccg ccactaactc
    11161 ccaactagat agattatgat actcttttag agtcttttgg gctgctgcca tgatacctgc
    11221 acctggttcg atccctgtga ttttttgatc ggcttgtttg cttaagtcct caatactatt
    11281 aacatctgtc atgtattttg gaacagctaa accaagtttg gtccctttta ggttaggtcc
    11341 taaatcatct aattttgact tgtatttctg atactgttgc ccatgagtta ctggcaacca
    11401 agcactagtt gaaaaatcag cgttcccatt tgcaacggtt tgccacataa cggcattgtc
    11461 aagcggagtc aaggtcacat ggtaaccttc gtttttcaaa acttctgcaa tcacatgagt
    11521 tgaggcaacc tcagaatccc actgcacata agcgatgtta acagtttcac ccttatctgc
    11581 cataccagaa gtcatagcca taataccgcg tcctaaagca gctatcaaaa agacagcaag
    11641 ggctgctaga ccaatccatt tattggtttt gccagctttg gctttttctt gaggtttgct
    11701 attgaacaac tgtgtcatac ggtcaagcac aatcgccaag ataaccaagg caagaccact
    11761 gacaaatcca ctaccaatat cagcgtgttg aagggctgac aagacttcac gtcctaaacc
    11821 aggagcacca atcatggaac ctgttactac catggagagg gctagcatca tggtctgatt
    11881 gacacccgcc atgatggtat ttttagctaa aggcaattct accttgaaga gcttttgttt
    11941 cccagttgac ccaaaggcat ctgaggcttc aatcaattct gttggaatgt cacgaatggc
    12001 caaatttgta aaacgaactg ttgggggcaa ggcaaagatg actgaagcaa atactccagg
    12061 caccatacca ataccaaaaa aggcaacggc aggaatcaag taaacaaaag ccggcatggt
    12121 ttgcataaag tccaaaatag gattcacaat ctgacggaca gtggcatttt tagccatcca
    12181 aatccccagc ggaataccaa taagtaccga aatcaaacta gctactaaca ccagagtaaa
    12241 ggtattcatt agctgcttcc ataacccttg attgtaaata aagagcaatc ctaacaaggt
    12301 aaaggttggc aaaggccatt ttttcttagc aaggaaaaac atccctgctg ttactagaac
    12361 aataaaaagt aacgggtgga taaataaaag tgttttggtc atccaatcca tcaaaaagct
    12421 accaactact tgcataatat caaatagacc tgagaaagtt ttagttagcc actccgttag
    12481 ctgctccacc agttgagcta ctggtaattt tgtttgtaat atcgtttcca aaatgcatca
    12541 ctccttttag ttaaggtctt cgtctgagat attagccaag gcttcaatca ctcgtcctcg
    12601 aataatgaca cctaacagac gattattatc atctgtcact gcaatcggag cagatgaatc
    12661 atagataaga ggcaaaatat ctgtaataat agtatctttt gagacagttc tcacatcgcg
    12721 atcaatcact tctgatagcg gtaacccttt tttgcgcgcc tctatggcgg catcggccgt
    12781 caaactaccg actaattggc ggcggcgatt cgtcgccatc aacatagaca cctcttcgtt
    12841 gtgcatacgg ttcaaggcaa cttgaggtcc atctaattca acagtagttg ttaacggttt
    12901 gatcatgata ttttgtgctg tcaagacttt agaacggtcc acatcctcaa cgaattcacg
    12961 cacaaagtca ttggctgggt tagtcaagat ttcctcacca gtaccaattt gcataatttg
    13021 tccgtctttc atcaaagcaa tccgatcacc aatccgcaag gcttcattca agtcgtgact
    13081 gataaagatg atggtttgtt tcatgctatc ttgcaaatca agtaattcat cttgcatctc
    13141 acgacgaatc aaaggatcaa gcgctgagaa tgcctcgtcc attaagagaa ttttagggct
    13201 attagctagc gcacgggcta ggccgacacg ctgctgcatc ccaccagata gttggtttgg
    13261 gtactgatct ttaaaatcta ataggcctga attatcaagg gctttttctg ccaatcgctg
    13321 gcgttcttct ttgggaacgc cacgtaattc caaaccaaat tcggtatttt ccaaaatggt
    13381 tttatgagga aagagggcaa agctttggaa aaccatgtta atgtcatggc ggcgcacctc
    13441 acgcagctga tctgctgaca tggttgagat gtctttacct tccagcaaga tagatcctgc
    13501 tgaaggttca atcaaacgat ttagcatgcg gacaagggtt gatttcccac ttcctgatag
    13561 ccccataata acaaagattt cacctttttt gacctcaaaa ctagcgtcat agacacctac
    13621 agtagcgcct gttttcttaa aaatctcact cttatttttg ccagtcttta ccatctcaag
    13681 agctgctttt tgttttttgc caaaaatttt actgagatgt ttgacttcta aaattgtttc
    13741 cattcgtttt ttctcctagg taatcgttta tcatgtgaaa tagtgacata gctttggcga
    13801 aatattagcc aatatttaac aaagtagctc tacacaatta ataaacgtgt ggtcactttt
    13861 ttacttaaaa aaagaatctg ccgaaacaga acataactat attatcacgt gtggtcactt
    13921 tttgtcaagg acaataactc gatctggtaa tgccacaatg ataggtgatc gctttcattc
    13981 ttttaggtat ggtcttttaa ccagtacaat tttttatcac aactgatgac tatttctttg
    14041 ctgattaggc tattgttaca ttagaaatcc ttcttttacc tataatatct cttttgcggc
    14101 catcgtaaat aacgaccacg gtgactctta aacatttgct catctctcca aaaaactcac
    14161 ctaagacagt gtcctatgaa gcgagttagc ttaatcaggc actatcatct taggtgagta
    14221 gggtgttgct tatagtaaag ctttaaattg gatattagaa tcttctaaga agcagtcatc
    14281 aaatccacga ggatgatggt attcgatacg attttggtct tgtggatagg tgtacttacc
    14341 accaacctcc cagataaagg gatggaaatc atattggaga cgttctttgc gcattttcca
    14401 cagttcaagg atttcatctg ttgatgccat aaagtttgac caaatgtcat aatgaactgg
    14461 gataacgact ttagcacgga gattttctgc catacgaaga aggtcaacag aagtcatctt
    14521 gtcttgaatc ccaattgggt tttccccata gttatttaaa acaacgtcaa tatcataatc
    14581 acgtccatgt ttagcaaagt agttcgaaaa atgagaatcc gcaccgtgat agatggtacc
    14641 acctggtgtt tcaaaaatat agttaacagc tttgcgagcc atgtcatcat ctgtgatagc
    14701 taaacctgct aaatcaccat cttgagcatc agctccttgt attgggagag ttaccagaca
    14761 ggtgcggtca aacgactcta cagcggtgat cttaatatct ttgaattcaa agctatcacc
    14821 tggctttaag atcataatac gatcttttgg cacaccccag tttttccaaa cttcaccaca
    14881 ttcataaggt cctacaaatt taacatgatt aagttttggg ttattgataa tggctgccgc
    14941 agtattgata tcgatgtggt cactatgata atgagaaacc aaataataat ccaactcatt
    15001 aatcataaat ggatcaatca ccattggttg ggccctaaga ttgggctgta atttacgagc
    15061 tcctgccata ttagccattt gatgtccacg aaccatatcc ttaacttgct tggtcgcttt
    15121 cccacgattt gaccataagt ccatgacaac attagcaccg cctggtgtct taatccagat
    15181 accacagttt cctaaccacc acatcgcaaa gttgtcagct ggcacgactt cttgctcgat
    15241 ttcttcattt aaccatgttc cccattctgg aaacgtgtta aggatccatg attcacgtgt
    15301 aatatcttgt actttagtca ttagggtttc ctccgaattt gttcttcgtt tctatacttt
    15361 aagtatagac tttcttgtca atgataaaaa gaccaagtgt cacacagatc tgtgttcaaa
    15421 agtggactga ctttttcatt agctcagtct aatactgtct cattttgcac actgtatctc
    15481 gcatcaatac tgtattcaaa gctatattaa aaacctcagt tatatttttc tatgtcatag
    15541 aaaactgagg tactttgacg acttaacact tactcatagc agctcgcgat gaatgagttt
    15601 ttcaatttta gaaaccaagg cataacgatc tgattcatct tttacaacag cttcaatagc
    15661 ttttgtgagc tctgtagaaa agcgggagtg ctcactcaag cgtccttgct ttgaaaagcg
    15721 tatcaatttg atgatatcat cgtcacttaa aatagggtta acaataagca tcgggatttt
    15781 agtctttagc gtatctgtag tagcaacaat catatcgact gctaaaagat cgtaaacact
    15841 ctgatactgt tctgtcgtaa aaacagcttc aatctgacca tttgctagat acctttgaca
    15901 ctgtttgaac aataactttt gaatgcctat cccatcatct gaaacaataa cgagtttggt
    15961 cttttcttgc tctgtattat tgtgacgcag ctctccccca agatggatag tcaagtaggc
    16021 aatatcatcg tctgtcaagc taatcgtcca atcttgttcc aaaatagtgg cacaagattg
    16081 agtcatagca aacaattctt cgtatttttc tttaatatgg tctgttaagg gattaactaa
    16141 aaaaatccca taagctttac ggtaaacgag cgccttacag tgggtcgtca actgcttcag
    16201 caaatcctgt ttatgcgtga aatgtaactg ataacggctc tccaactggt caataaagtg
    16261 actaatggtt gctcgcatgt catcataatc ttgactttcc acgtgatgat cttgatcttt
    16321 tcgaaaagac aacatcaaca tcgctaccat actaacttcg atatcatcca aatgaagctt
    16381 aaaattatgg taaagctctc tagctaagtc ctgagcaatg tgatattctt tacgtttcca
    16441 aatcaaatga aattcttgtt taagagcaga ctttgtttct gaatcaagac gcatattacg
    16501 ataacttaaa agcatgaaag gtaaaatctg caacatgaat tggctatctt gggtattaat
    16561 tgtctttcct aaactagctt gagataaaaa aagatactca tgaaaataag tcaaaatatc
    16621 tttagaaaaa taaaccgata agccttgtat ctctgagagt ttacggttga atatatcaat
    16681 aaagctagta ttgccacctt ggtaaacgtc cactaacaat ttgtacaggt attgaataag
    16741 agccattgga tgacaaccaa aataatatcc cctagccttt gtcgcatgaa gctgaatctt
    16801 gtattgtttg tcttccagtt cttcccttaa ctcagtcagg tcatttaaaa tcgtgttacg
    16861 agacacatca ttgatctgca tcagtttatc aatggtcacg cgctcagtgg aaaccgcaat
    16921 ataaatcgag gatagtcgtc tgcgctcatc acttttcatg acataattgt aatcagtcac
    16981 ttcttctaat aataagcgac aagcagcttt ttgatccgcc gttaaaagaa tgccaagtct
    17041 aggataagag ataatctgat ctactccctt tggcaaagcc tgattaattt tgtcaagctg
    17101 gtaataaact ttacgacgtg actgatttaa agcatgagaa atggccataa ctgtctcagg
    17161 tgtttctagt ttaagtaaat aagacagcaa gtcataactc tttttatcta aaatcatcat
    17221 gtttacaaac ctcctctctg aaaatccatc cttgcttatt ttatcatgat ttcagtgact
    17281 gcgcagccaa gttttgaaaa actcaccata ataagattca cttaatatga ttcgagctaa
    17341 tcacctcaac tgatccaaca ccaacacctt atatctggtc tctaagaact ccacaacata
    17401 cggttcatcc agtttgtgct cattggcgtt taaacagttc atcgaaaagg acaagtcaaa
    17461 aggacttgcc caaacaatga ttaacaaaac cttgaacttt ttggcttcta gtgagaacct
    17521 gtgctaataa gccatatcgc gacctcattt ttgaccgtaa taagcatttg ggccatgttt
    17581 acgcaaataa tgcttatcca taatgtatcg tggcgctggc tctacgcgtg gattgatttg
    17641 ttctgtcaag cgattcattt tagccacttc ttctaagata accgagtggt aaacggcttg
    17701 ctctggggtt ttcccccacg taaatgggcc atgattacgc accacgattc ctggaaccgc
    17761 cataggatct aagcctcgct tgctaaattc ttccaaaatc acgttgccag tttcttgctc
    17821 gtaagcccca tcaacttctg cttttgtgag agaacgagca cacggaactg gtccatagaa
    17881 gtagtcagca tgggtagtcc cataaaacgg aatgtcacga cctgcctgtg cccaaccgac
    17941 agcttctgtt gaatgagtgt ggacaatacc gcccacttct ggccaagctt tataaagttc
    18001 cacatgggtt ggcagatctg aagaagagtt caaatcacct tcgaccacat tcccatcaag
    18061 gtctgtaaca accatatttt ctggtgtcaa gaggtcgtag tccaccccag aaggcttgat
    18121 gacaatacgt cctaattcac gacaaacttc agagacattg ccccacgtaa atttgacaag
    18181 accgtgttgt ggtagagact tgttagccgc acagacacgt tcacgcattt cttgcaagtt
    18241 tttcgccata ttatgtcaac cccgctttct caataagtgg gtaaaggaag tcctgagctt
    18301 ctttgatagc agcccttgtt tcctccaccg tgtcacaatt ttctgaccac atttcaatca
    18361 aaaaaggccc attgtagttg gttttcttga ggaccgcaaa cagctcttgc cagtccacac
    18421 aaccttgacc aaatggcaca tcacgaaact gcccttttga ggtttccgtg acagcataag
    18481 tatctttcaa gtggagagct gcaatggact tgtgaccatt gtaaaattca ctccacaaat
    18541 cattatgcca ggcagaaaca ttgcctgcat caggataaac aaagagataa ggcgagtcaa
    18601 tctctttttc aactgccaag tatttctcaa tagagttgat aaaagggtca tccataatct
    18661 caatagaaag tatcacttgg gcttcttcgg cccagtcaca agattgtctc aagtttttga
    18721 taaaacgagc acgggtttca ggcgattttt cttcatagta aacgtcataa ccagctaatt
    18781 gaatggtacg aacgcctaag tcctgtgcca attcaataca ttgtttcatc agtttaagag
    18841 acttggcttc agtagcagaa tcatttgagc ctagtgggta acgtctatga ccagaaaaac
    18901 aaatggtcgg gatgcgaatg cctgtttcat aaatggcttt cactaaatca agccgttctt
    18961 ccttagtcca ctctaagcgc gctaagcgag catctgattc atcaacagac atttccacaa
    19021 aatcaaaacc taagtctttt gcaaattgca agcgttcacg ccaggtaaac tgttttggcg
    19081 ttgctttttc ataaatacca ataggacgcg ccataaagtt acccccagat acgtttgatt
    19141 tcatctttga aggcacgcgc cgctgcagca ggatcttctg cttcagtaat gcctcgccct
    19201 gcgataaagg taaagacatc aaccccttca aaaagtttca aagtgtctac atcaagccca
    19261 ccagttactg agacacgaaa gcccatgtca atcaatgttt tcaccttgtt aaggtctttt
    19321 tcaccccaag tttctccagc aagaagggca tcacgagatt ggtggtaaat cgcttgggaa
    19381 atcccagcgt caagccataa ttgagcttgc tcataagacc aatcaccata aagttcaatt
    19441 tgaatctccc cacggtctcc gcgcacttct tttatggctt tgagggctgc ttccatggtt
    19501 ggaatcgtcg cacagcaaat gcaggtcatc cagtcagcac cacgttttgc attgttttta
    19561 gcgaccgtac cgccagcgtc agcacatttg gtatctgcta cgataatttt ctctgggaag
    19621 aggctgcgta atacctcaac tagttcgctt cccacttgaa gcaaacaaac ggtaccagct
    19681 tcaatcacat ctacttcgtg acccacagca acggctgctt taacagcccc ctgtaagtca
    19741 gaatggtcta aagcaacttg taaatttgga atatgtgtca tctctttttc ctaaaactct
    19801 tagccaccgt gcaaactggt taagagttct tatcctttcc atcattcgtt agtatcctgt
    19861 gtaaggaaag cttaagcatt caagtccata ccttctaagt aaggactatc tttagattct
    19921 tctaccattt ctagaacctc tttaggactt tggcaggcca ccaagcgttc aatggcgtta
    19981 tctagttcaa acaaggccac aatttgtggg atagccactg ttgtgtgaat agaagggtct
    20041 gtagcggcca acgtcaaaag aacagaaacc tctttaccat ctgagaaggt gactggtttt
    20101 gttagtgtaa tcaaagcaaa tgcattgcga ttaacgccta aacctgcttc tgcatggggc
    20161 atagccatac ctggcatcaa cacataataa gggccgtatt tttctgttga tgcaatgatg
    20221 gcgtcgtagt aagctgacgt cactgctttg ctatcaatca aaggttgaac agctaatcga
    20281 acagcctctt gccaagtatc agcacttagc cccaatcgga tggagttgtt gtcaataaag
    20341 gcttgtttta aattcataaa aactatctct ttctagtgtc ttgctttgca acacacacca
    20401 tttttactag ttttatccta ctaaagagaa agagctgaaa accttcgtca caactccttc
    20461 gttttgattc agaattagct tatagcactt cttgtagttt ggttttaatt tcattatcat
    20521 ccatcaggtt atcaagacca accaagtggc ctttggtacg accatcaagc tcatgaatca
    20581 agtggtttga tgccactaca atatcataac cagaagctag acctttagct tcaccaactg
    20641 agcaagaagc tgactgaata tctgtcacac caagttgacg aagcgcattt tcaactttca
    20701 ttttgatcac catagatgac cccataccat tgccacatgc tgttaagact ttaaccattg
    20761 ttttttcctc cgaaaatgtt ttattattat tttgttagaa tagcagagct atttcttaag
    20821 atgcagtgtt attgagcttc cccacggtaa taagcttctt tgtccttagc ttttgcgaat
    20881 tgcaattgtg gaatggcaag taagaagatg caaacaagca cataccctgc aataccaagg
    20941 aatttgaaga ggtacccaaa tggtaaccat ggaagaacca agtcgatatt accgtgataa
    21001 ccacctgtaa gtcctaaaag acctacagca acagcaccaa gtgcaacttg caaaatacct
    21061 gagataaatg atagagctac cgctgctttc cagccaccac gtttatctgc ataaaccgca
    21121 attgccgcat tatcaaagaa tacgggaacg aatcctgtga taattaagat tggattttta
    21181 aagataacaa gaagagcaat ggtgattaat tgaccaatca aaccaaaggc aaatcctgaa
    21241 agcaccgcat ttgaagaacc aaagccatag gaagctgcaa cgtcaaccgc tgggaatgac
    21301 cctggaagca atttgcttga gatcccttga aaggcattgg tcaattcaga aacaaacatg
    21361 cgaacaccct gcatcaagat aaagagataa actgagaagg tgagagaggt ttgcaaaata
    21421 tacatgaaga aagcttgctt agctgggttg aatgctccag gtccgattaa gtccacatca
    21481 gacataatgt ctggaccaag gacagccaag atagctccga aaaagaccaa catcaaagtt
    21541 gctgatgcaa caacagtatc atggaaaatg tttaagaagg ttggtaattt taggttatca
    21601 aggttttctt cttttttacc aaagaacggt gcaaccttat ccacaaacca aatcgcaaat
    21661 tgttgttggt gaccaatcgc aaacccaccg ccacttgtaa ggcgttgagt tgcttcaacg
    21721 gtcatgtttg agctgactgc ccagtaaagc ccacagatga taccaactgc ccacgcacca
    21781 aaggcatttt ggaattgagg gattaaaaga aggacaaaga ccgaaatcgt cgctgcctgt
    21841 tggaccatga tgtgacctgt gatgaaaagg gtacgaactt tagtgacctt gcgaagtgca
    21901 accaaaagaa tgttgacccc aaatccaatc aagagagctg tcgtagcaac actgatgaag
    21961 cccatctctt ctaacttagt attagcagca gccaaaccaa aataaggatc aataacggcg
    22021 gctttcaatt caaatttctt ggcaagagca accaagatcg gacgaaaagt cgttacaaga
    22081 ccgcctgctc caacgttcaa aatcaagtaa ccaacggtag cctttacaaa accagcaaag
    22141 acctcataaa tgggtttttt aaggagtaaa tacccgatta aaaccaaaag acctacaaag
    22201 aacgctgggt tttgcaaaat attttgcgag aaccagttta ggggagctag gagcatttcc
    22261 ataagaacta cctcttttca aagttaataa agataaagtg tttatatgtt cattataggt
    22321 aatgaaagcg ttttaaaaaa gaccaactat cacacaaaga agtgttcaat attagactta
    22381 agttaactta ttttaaaatt taactacact taataataag gagaaaatac ccctaaacaa
    22441 cgattaaaaa ccacaagaac cttcgttctt gtggtttttc tagataagtt gctttttgaa
    22501 tagatcagct ctttggcatt aactgtgacg tgacgcaaat aaccactgaa gggctgtgtc
    22561 acctcaaact atttgataac aatattaacg agtttgtttg gtactgcgat gactttgatg
    22621 atgtctttgc ctgcgatttc ggcttggact ttttcgttag caagagcaac ttcttgcaat
    22681 tcttcgcgtg aaaggtcttt agcaacgaca agtttagctt tgaccttacc tttgatttgg
    22741 acaacgattt ccacatcgtt ttcaaccaat ttgctttcat cataacttgg ccatggcacg
    22801 taagagattg actcacctga ggctgtcaag acttgccaga gttcctcacc taagtgcggt
    22861 gcgaatggtg caatcaattg aacaaagcct ttggcatagt cgctaaagag tttgtcttcc
    22921 ttgttggctg cattgacaaa taccatgagt tgagcgatag ctgtgttgaa tttcatttgg
    22981 tcaacttgct cagtaacagc cttaacagtt tcgttgtaaa ccttgtcaag ggctccgcta
    23041 ttttcctcag tgatctcttt agttgtgatc aaacggtaaa cacggtccaa gaacttacgt
    23101 gagccttcaa gaccttcttc agaccaagcg atggaagcgt caagtggtcc catgaacatc
    23161 tcgtaaacac gaagggtatc agcaccgtac tgctcaacaa catcatctgg gttgacaacg
    23221 ttcttaagtg atttagacat cttagctggt gcttgttcaa gctcttcccc agtttccaca
    23281 tggaagaagc taccatcacg tttttcaacc ttgtcagtcg ccacaagagc accacggcta
    23341 tcgcggtagc ttgtccccaa aatcatccct tggttaaaga gtttttggaa tggctctttg
    23401 gtaggtacaa caccgagatc gtaaagaacc ttgtgccaga agcgagcgta gagaaggtga
    23461 agcacggcat gctctgcacc accgacatag atatcaactg gcaaccattg tttgaggagt
    23521 tcctcgtcag ccagtttttc agtgttgtgt ggatcgatgt aacgaagata ataccagctt
    23581 gaacctgccc attgtggcat ggtgttagtc tcacgacgac ctttcacacc atcttcacga
    23641 gtcacttcca accagtctgt cacgttagca agtgggctct caccagtacc tgaaggacgg
    23701 atatccttcg ttactggaag aaccagtggc aactcactct caggaaccgc tgttgaggtc
    23761 ccatcttccc agtggatgat tgggattggc tcaccccaat aacgttgacg gctaaagagc
    23821 cagtcacgaa gacggtaagt gactttctca ttaccgacgc cttctgcttc aagccattcg
    23881 accattttgg caatagctga cgctttgtca agaccatcta ggaagcctga gttgatgtga
    23941 agaccgtctt ctgtaaaggc agcctcttca acattaccac cttcaagaac agggatgatg
    24001 tctaggttaa attgttttgc gaattcccag tcacgttcat cgtgggctgg aacagccatg
    24061 atagcaccag taccgtagct ggcaaggaca tagtccgcaa tccaaactgg catttcgtta
    24121 ccattaactg ggttaatcgc ataggcacct gtccacacac cagttttttc tttggcaagg
    24181 tctgtacgag caaggtctga tttgagactt gcttggcgtt tgtattcggc aacggcttct
    24241 gcctggtcag ctgttgtaat agcatcaacc aaggcatgct caggagcaag aaccgcgtaa
    24301 gttgcaccaa aaagggtgtc aggacgtgtt gtaaagacag tgaagttctt atctgtatcc
    24361 ttaaccttga aggttacatt agcaccagtt gatttaccga tccagttgcg ttgcatgtcc
    24421 ttgattgatt ctggccagtc aacttcttcc aagtcttcta gcaagcgctc tgcataggca
    24481 gtgattttga gcatccattg gcgcatgggt tttctgacaa ctgggtagcc gccacgttct
    24541 gatgtcccat ctggaagaac ctcttcgttg gcgatagctg ttcccaattc ttcaacccag
    24601 ttaacgggaa cttcagcttc gtaggctagg cctttttcat aaagcttggt aaagatccac
    24661 tgtgtccact tgtagtagtt agggtcagtt gtgttaactt cacgatccca gtcgtatgag
    24721 aaaccaagag cattaatctg gcgtttgaag ttagcgatgt tttcagctgt aaactcggct
    24781 gggtcattac cagtatccat agcgtactgc tcagcaggaa gtccaaaggc atcccaaccc
    24841 attgggtgga ggacattgtg accttgggca cgtttgaaac ggctgagaat atcagttgct
    24901 gtatagcctt ctgggtgacc aacgtggaga ccagctccag atgggtatgg gaacatatct
    24961 agggcataaa acttaggttt tgatgcgtct gttcctgttt taaaagtatg attatctgcc
    25021 caaaaggctt gccatttggg ttcaatagcg gtgtgatcgt aaaatgtcat gtgaaatctc
    25081 ccatgtaaaa aatctagtct tcttattata gcatgagatg aagcttttag gaatggtcaa
    25141 gacatgacta gcaccttggc ttttgagact tcagacgatt agctattttt aaaccctact
    25201 ttagcatcaa ccatcctcac aaaaagctaa taaaaacgcc agacttgctc tgtaaacaga
    25261 gccaatctgg acaaatagac ttaaaaagca agtgcttgct caaaatcggc caataagtct
    25321 tcgatatttt caacaccaca agacaagcgc agcaaaccat caacaacccc ttgttccaac
    25381 cgcgcttgtg atgataaaca agcatgagac atcgttgctg gatgtgacaa aatggactct
    25441 actccaccta gactaacggc taaaatcggc aactgaatac gatgggtaaa ggtcatcagc
    25501 tcctccttag aagccaactc aaaggataaa acagcaccac cattcttagc ttgttgcagg
    25561 tgggtgtcgt gattaggatg actgtctaag ccagggtaat gtaccttaag aactttgggg
    25621 tgcttcgcta aatagttagc gagctgctga gcatttttta ccgcttgttc catacggatc
    25681 cccatagttt tcatgccacg aagaatcagc caagcgtctt cgaccccaac aattgctcca
    25741 aagttttttt gaaaaagctt gagctggttg tagatagcct cattattggt agcagctaaa
    25801 ccagctacca catcactatg accattgata aatttggtca cactttctac cacaatatcc
    25861 acacccatag ctaagggttc ttgatacaaa cttgtcataa aggtattatc agccacagtt
    25921 agcacaccat aagctttggc gagttgcaca agctctgaaa tatcacaaat tttcaagagc
    25981 ggatttgacg gtgtctctag ataaatcata cgcgtattag gcctgatact tgcttttact
    26041 gttgctaaat cagccatatc tacaaagctt gtttcaatct gataattggg taaaatagct
    26101 gtcgcaaatt ggcaagtccc tccatagacc tccaatggga aaatcacatg atctccagct
    26161 tttaagagca tcaaaacagt tgaaatagca gccataccag atgcataagc caccgcataa
    26221 gtcgcttttt ctaaacaggc taacccgtct tctaaagctt cagtcgttgg gtttgaaaag
    26281 cgtgtgtaca aatgtttttg actaggacaa tacagttctg aattgtggaa cgtcgaagtc
    26341 tgatagatgg gaactgaagc tgctcctgta aactcgtcaa taaccgtata gccatgtaat
    26401 aaggtcgtat tttctctcat ttcgcgcacc tccgcaatag cgttaactcc aatgaacttt
    26461 cttttagaca atggtcccac cccaatggtt gcacaaacca aaaagctctt caaaaaagcg
    26521 tgatctgcta acctataaga aaagtagtgg gcacctttta aatggataat gaatttttcc
    26581 aacaatatca tttttagatc gattacgttt attatacaac ctttagctaa gtttgcaagc
    26641 ccttttatca tctttgacca agctgccaac acctctccag caaattaaag tatagctacc
    26701 tttggcccca tcgtctcaaa agctcagctt atcacactga cagtcctact ttgcatcgtc
    26761 acccactcaa tagggcaata ttgccatcaa aatatcatta aaaggtcaca atttatttca
    26821 tttaatgaga aacagaactt gttttcacca tacgtcttat agtacactgc taaagtacta
    26881 tttattttta ggagagaaat atagtgaaaa acaaattatt tttagttgcc cttgcgaccg
    26941 taactgtcct agggccgtct ttagcaaccc ctcatcacca gaccgtgcat gcggcggaac
    27001 gctgtacaca cgtatctgat aaccctaatg tttttctatg ttcagatgat ggaaaaccaa
    27061 ctatacagac taactccgtt ccaaatccaa ctcaaaacca aaaaagtggg cccttggatt
    27121 ggatttggaa cactattaag ggctggtggg aacaggtaaa aagctggttt tcgtaatact
    27181 gttcacagca tagtctacca ctaactatta aaataaaaaa ccatctcgtg gcggttttct
    27241 aatcctaggt tgctacactt cttattcaga taaataataa taataaaaac attaaaataa
    27301 cacttgattt attaacaaaa agttagtata atatagtcat actacaaatt aaaggagtta
    27361 tctatgaaaa agaaattatc tttatttatg atcgcaactg ctgctgttgc aggccttagc
    27421 cttgcaacac taggaagcca ccaaactgtt tcagcaaatg atgctagcta tagtaacggt
    27481 ttctacggac acaatggtcc tgacagctgg ttactttaca cagtatggta aacctcatac
    27541 cgtaacatag aagctatctt cttcgtaaga ctacggttat gaaaagccta ggactgcata
    27601 agcagcctta ggctttttga ttggctaaaa cgtttttgcc aatgacacct caaggtaaaa
    27661 cagaaaaggc acactgccta taaaaggaca agtccaaaac acaagtcgta acagttgcta
    27721 gctaaccagc accaaaaaag ctgtaacacc tcaaatagga tgataaccag attaatataa
    27781 ttaatcatta atgtgattta tataagataa agaaatgctt tattcttggc tttctttgcc
    27841 tttttcggtc aaacacatca caaaaagatt aaaatgtgac gttgaatcat taatagaata
    27901 tgagtataat ataggtatac tataatttaa ggagttacaa atgaaaaaga aattatcttt
    27961 atttatgatc gcaactgctg ctgtcgtaag ccttagcctt gcaacaccag aaaaccaaac
    28021 tgtttcagca aatgaggcag tagctactca agcatgtaat tacgattttt acggatattg
    28081 cggacctatc agctggttat tctataagac aaaacacttg ttgatgggag agcagcctac
    28141 tactactact tctacgacta cagtcacaaa atagtgagac aacagttata aaaaagccca
    28201 ggactgcgta agcaggcttg ggctttttag ctgtcctaaa gtcagggtat tgccacaact
    28261 tcaaacccaa aaacaattaa accgcacccc aacaattaga cataggctat gtccccagaa
    28321 tcatggacac atatcactaa agcctaataa ttaaggtgcg gtttaaggtg tggttgcaaa
    28381 gaactgtttt taattcattt ttttatgaca tgctttgtgc tgttgtagtt gcctggttat
    28441 tggggaagaa tcgctgtagc caataagaga tcaaatccca tataaccttg accggagtca
    28501 atacgggatg ctgagatttc cagctttcat tataaccctc tttgtaccca tctgtatacc
    28561 cttctttata agtgccttta ttggcctctt caatatcact aggtaattct gatacttcag
    28621 ggacagttgg ggtcaaatca gtccaagtag ccttttgacc agcttctttc ccttttgtca
    28681 aaccctggtt atagcctagt tcccacgaat ctttttgttc cccatctgcc ataacaggtc
    28741 ttgctataag caacatagca gcaaatagag ccaccaaaag gtagacgaag cgtagaggct
    28801 ggtaagattg ttgtttcatg ataatagtta tctctccctt ttatgttatg ctttgcttgt
    28861 taaaaagcag cttgttagta ttatagatta gaagcagctt agattcaagg tcgagatgtg
    28921 agaaaaaaat aactttttga ttagtatatc aaaaaagctt gtctatgaag gtatctcata
    28981 gacaagcttt cttctgtatt tgtgaaagga gtaggcacaa ggcctcaaga gataagcagc
    29041 atcaaggtgc ttgacgaacc tcttgaacca gtcctactta taagtaatcg aaccatatgg
    29101 gctcagggtt gatcctgaga tgttgacatt gatttcttta gacagtttca catctctttc
    29161 gtcgatcact tttcgccacc attcccaagc taagccggtg cactctctag ccatgatacg
    29221 gatatttctt gaattagctc ctagtgggat aactgtgcta aatggtgatg tcttactata
    29281 ccagttgttg tcccaacgtc gttttgtaat cacttctttt cctttgtcat cataattgat
    29341 ttcatcccaa aggatttcat attgagcaac atacgcacct cgatgagaca ggttaatttt
    29401 tccactagtg tactcggtag atgttgtttc aacgtattca gttctgttat tgacacccgc
    29461 aattttatta tttttaagga aaacactggt gtatgaaata ggataagctg ggttttttct
    29521 actgaaggta gcattgtctt tgataacgtt tctaataaca tcaaagtctt ttgtgaccac
    29581 cttattgtgc tctgcagcat ctcctcctaa aacgacagct gtaaatgagc tattttctaa
    29641 gatatcagag tattttccat tcgttttaac atctgttcct tttagagctg cactaaaggc
    29701 cgcttcaaca tcattacttt tagaacttgt ttctagtttg acaaaaacag ttcgaccata
    29761 ggctacgtta ctcacaaaga gtggcggagc ttcattgctg acaccttttc gttgcaaatc
    29821 tttaaaggtc actgatttat caaacacatc cgcaggatta ttaggaaggt ttgctgatac
    29881 ggtgtaaaaa atttgcttgt atgctgcaat catcaccttc ttttcacctt ttgaaatcga
    29941 cttgaaatca atgcctaaag taccatctaa gattttgcta ttaacattta gagctgcttc
    30001 aatctgtgac ttagaatata ccattgattc agtatattgt gttctggcag gaagcgtatt
    30061 accaccagaa taattatcat gccattggtt aacaagatta tcaatagctg ttgaaacatt
    30121 ggcataggta gggtcattga cctcaaccgt tgctttttct cccatacctg gtaaatcaat
    30181 atggattttt tgtgggtttc gcttggtgac taccgcgtct ggtttgtttt cggtaaaacc
    30241 tttatcagcc agctgaaggg ctgctggata ggtcatatca gtgacagagt caatgatgga
    30301 aatatcgact ggtgtagtgt tgatattttt tttctttctt tcaatgacaa taaatttatc
    30361 agctttctta acgccttctt taggaacaaa attttcaatg gtttcaccat ttttagcaag
    30421 tacttcaagc tcattataat ttagtgaata aatcttgtca ttgatttctt cagtgtgatc
    30481 ttcttcgctc ttttttttgt cttctgactt tttttcttct ttttctgcag attctagtgg
    30541 catttctttg ggagcaagct taatcatatc gttagagtta agcatatcat ccattttctg
    30601 acctgctttt tcgatagtta gctcactact ttctggtttt ggttgctcac ttgtcgttgt
    30661 ggtttctgta ctagcagtgt tttgtttgtt cgattcagca ttagcagtaa caagattacc
    30721 aatgataaga gctgccgtta gtagcccagc gacgcgactg tattttttaa atgttttttt
    30781 attagacatg tccttcatac ctttttatca ttctaaaatg tttctattgt tctttcgacc
    30841 atatcaagca tagtatcttg atagtgagca aattctccta atttgtaatg tgccatagcc
    30901 tctctaatat gcgcatcttt taacgctttg gtcatattag ctactatgct ttgacggttt
    30961 aaataaaatg gcaagctttc gtaataattt aataacatgt tttcagcgtt tagggtttga
    31021 accgcttggt aaatgtctaa tgcttgaccg ctatttgaca tttctaataa tgcttcttcg
    31081 atgttagctt tcaattgggt atagcctttt atgaaagcag ggtaaacccc atactctaaa
    31141 ataacttctg gatcagaaat atctcccata taagagtctc tcctagtgat acgatactct
    31201 ttgtcactac ctatcaagta tttttttaaa tcattaacag ttacttcttt ttgaaacatt
    31261 ttttggtaat gttctaaacc ctttggcacc ttatacatat tgttacttcc tatcttgcat
    31321 tttcttaatt ttgaagcgac cagttacttt tttatggtca ccatttggag caatgatggt
    31381 aggatcaata atttcaaaat agcctttggc atcaatctct gctaagacag ctttagattt
    31441 tagatcttta acactaatcg atgggaaaat taattcactt tctgaatttt caatgtgttt
    31501 tcctgaccac gtttggcaac caggaatgtt gagcgaattt gctccaggag tatctccgct
    31561 agctgttcgg atatagccta gcttatcaat gttttttgtt agttcagaat cgtactcata
    31621 ttgataaacc ccatcactat attttccgct atcgacacct tttatatttt caatttggtc
    31681 agctgatttt aacttacctt gatcagtaaa gagcaatgcc atatcttcag caaaacttgt
    31741 cacaaaaaga ccgttttctt gtcgaccaat cgttgacttt cttttagcat cgtcaaaagc
    31801 taacatcaca tcttttttgc ttgtaacgtg ggaagctccg ctttctttgt agacttgttt
    31861 tagtttttca acaaactctt ttttgtagtg gtatttgcgg ctaacataca tccggtaata
    31921 ctcagcacgt gacgcaacag cagacatctg aatagcttta tcagcatact tgtcggcagt
    31981 ttcaataaac tcatctgctt gtttatctgt tacccattta tctttaattt ctttaaactt
    32041 tttttcaaaa atatcgcgtt ctatattgcc aaaagtgacc tctgacaagg ctaaaatatc
    32101 cttgttttgt ccctgatgga cctctgttac ctcaatatca atacgatcaa catctaattt
    32161 tttatcaatc tcaatgtgat gcggtttttt agtgtaggtg tcgcctaatt caattttgtg
    32221 aaagaggacc aatttattcc cgttatagta tttaacatca gcctttgcaa ttctattttt
    32281 cttgtagtaa ttctccttgc tagatgtatc tccattaaca attaaaattc tcccaatatg
    32341 catcggggac gcaaacttgt aggaaagtac ttcaccaaca ccaccaccag gtgaattttc
    32401 ctcccagaca gtactcattg agccgtctaa tgtgtgcatg acatgattgt cttctttaga
    32461 ggaagttgcg ttagcttcca taactttggt cgtttcatat ttgacatcgc tttttttatt
    32521 ttctttgcca ctaacagcgt acgttgtgct attagctaat aaactgctac ccattgctcc
    32581 agccaaagca atagcaacac ttgtctttgc gacaatatga gctaatgtta cttttttgtt
    32641 tctcatgtaa accaccttat attattttta agtaaactat acattagcaa atagtttttg
    32701 tcaatgcgat ttattaacat cgttttagac ttttctatca aaaaaatgaa cacaaaaaaa
    32761 caagtagcca actgttacaa gtttcaaggg gatatttcca tcttattatc acagaacctt
    32821 cacatctgtt ctatttaaac cagattaaaa gtactagtta aatatttgat agtagcaccg
    32881 ttatcagtaa tagatctgtt aattatctct gattacaatg atgttaacga tagtcatacc
    32941 tttgtttgta cacacgcttc acttccaact aaaaaaggtc tgatggtaat acttatacca
    33001 tcagaccttt gagagcctat agttctgcaa tttgataaag ttcaacttct gctactgttt
    33061 cagagccaaa catgttgagc atcagtttca ctttattgtt ttcgatttct accacacgac
    33121 cttcttgtcc cataaaggca ccgtctatga tctgaaccac atcgccttct ttgatattgg
    33181 tatcaaagac atcaatggtt tgacccatag acaacaagat agcacggatt tcttcttcaa
    33241 gaagtggtgt tggttttgat cggttcccgt gtgatcccac aaaacctgta acgtttggtg
    33301 tgttacgcac aacaaaccaa gcttcatctg tcatgaccat ttcaaccaag acataccctg
    33361 gaaagcgatt ttcttcgatt tctttggttt gcccattttt ttcaacgtta accgtttgtg
    33421 tcggaatttc gacgcgcaaa atattgtcca acatgttata ggtttgggca cgttgcaaga
    33481 ggttttcttt tactttgttt tcgtagcctg agtaagtttg gaggacgaac cagcccttat
    33541 caaatgaatc tagcatgatg tttcctttca ttataaaaaa gccctttttt tggcttttgc
    33601 gctgttttct atcaccttca ttataataaa agacagggct gctgtcaacg tatctgtgga
    33661 gctacttgac acctttatta aaaagtccaa agtcggttcg tgacgactct ggacggtatt
    33721 gtacagtttt agttttcagg aactgtgatg gtcccttcga taatcgcttt tttagcagcc
    33781 tcaatagctt ttttagtgtc ttgtgtcaga gcatccgttg taaggctaac accaccttct
    33841 tttaaaccaa aagtggttat ttgaccacct gggaattggc cttctgaggt ttttacggct
    33901 acttttacta aagcttttcc gacttccttg atacttgagg tcaaaacaaa attagctgac
    33961 ttgccatctt ttgtggtgta ttttccatct tcactttggt cacggtcaac accaataacc
    34021 caaaccttat cttcttcttt acgtttttcg ttgatagact tagcttcgct aaagacaccc
    34081 gcccctgtgc ctcctgctgc atgataaata acatctgcgc cttcagcgta ttgagcagct
    34141 gcaatcgtct tgcctttggc agcatctgca aaagagcctg cataagcaac tcttactttg
    34201 atggtatcat ctactgattt cacaccagct tcaaaacctt tttcaaagcg cttgacaaca
    34261 tctccttcca taccacctac aaaaccaact tgcttggttt ttgtcgtttt agctgctgca
    34321 acaccggcta ggtatgctgc ttcatggtct gaaaaggtga tacttgcaac atttttttga
    34381 cctttaatca catcatccac aattgcaaaa tggttgtcag gattgtttgc ggctactttt
    34441 tctacagcgt catgtaatgg aaatccaatc ccaaaaacaa gattataacc attggttact
    34501 gctgactcaa ggtttgtggt aaactctgat tcattggctg attggaaata atcatagcct
    34561 tttccttttt caagtttatt ttcttttccc caagcttgca agccttccca ggctgactgg
    34621 ttaaatgact tgtcatcaat acccgtctga ttagtaatca tagcgatttt taatgaattg
    34681 tcagttgttt gtttagcgga gttattggta cagcctccta atgtgaataa ggcagtcgaa
    34741 acaagaccaa gtgacattac ttttttgttc atgtgaaaat gaacctccta aatgtttaat
    34801 cctaaagtga tgtgtcattg ttaacgttct acttatgctc tagaacgaaa agtttagtat
    34861 atcaaaaaaa tcagttttat cctactattt ttgttgaaaa atctcttttt caccaaaatg
    34921 accgaacgat aaagtgagat caactggtaa atcccattat ctcctcaaaa atcacctctt
    34981 aaggagataa gtcaacgaca aaactcaagc tactagtaat agcatgacta actacacaat
    35041 ccaacaagcg gtaaaaagag ctggaaacgc tatccaactc tttgtcatac tagacacctt
    35101 agatagatgc ccagaccgac tcaagaatat tggtctgttc acgtcctgga ccaactgaga
    35161 aggttgaaat gcgaacacca accaattctc caacacgacg gacatagttg cgggcatttt
    35221 caggcagctc atcaaggcta cgaacacccg tgatgtcctc ttgccagcct ggtaattctt
    35281 catagattgg tttgcaacgt ttgagttgtt caagacttgc tgggtagtga tcaatacgtt
    35341 caccatctaa atcgtaagcc acacaaatct taaccgtatc aagccctgaa agaacatcaa
    35401 tggaattgag ggagaggtta gtaatccctg acacacgacg actgtggcgc atgacaactg
    35461 agtcaaacca accgacacga cgtggacggc cggtcgttgt cccgtactca tgacccactt
    35521 cacgaatgcg ctcacccact tcatcaaaga gttctgtagg gaatggccca tcaccgacac
    35581 ggcttgtgta ggctttacag acaccaacta ctttgttgat tttacttggg ccaacaccag
    35641 aaccaatggt tacaccacca gcaactgggt ttgaagacgt tacaaatggg taagttcctt
    35701 ggtcaatatc aagcataacc ccttgagcac cttcaaaaag aacacgttta ccagcatcaa
    35761 gtgcatcatt taaaatcaca gaagtatctg tcacatattg cttgatttct tgaccgtaag
    35821 catagtactc ctcaaagata gcatcaaaat caagaggtgt actgtcatac attttttcaa
    35881 ataaacggtt tttctcagct aaattaatgc gtaggcgctc tgcaaagata tccttatcca
    35941 gtaaatctgc gatgcggata ccaactcgag cagctttatc catataagct ggaccaatcc
    36001 ctttaatagt tgttccgatt ttattgtcac cttttgcatc ttcttgcagt tgatccagct
    36061 ggatatgata aggtaaaata acatgagctc ggtctgaaat gcgaaggtta tctgtcgtca
    36121 ctccttcatc atgcaggtac gccaactctt tgaccaaaga tttggggttg accacaacgc
    36181 cattaccaat aacggaaatt ttttgcggga agaaaattcc tgatgggatc aagtgaagtt
    36241 taaacttctt accatcaatc acgatcgtat gaccagcatt atcaccacct tgatagcgag
    36301 caattacttc tgcatccgct gacaagaaat cagtgatttt acctttacct tcgtcacccc
    36361 actgggtacc aacaacaact acagatgtca tatcattgtc ctaactttct gaaacactta
    36421 ttctcaataa aaatcatagg caagctaacc actgcttaaa aaccaaaaag aaggtcagat
    36481 ctgtaagatc agcaatgtag ctcttgatat ttaaagaaaa caggcaggaa tctcacctgc
    36541 tagcatatat cttacaactt attatagcaa aaaatggttt tttctgctag atttcttagc
    36601 attattaagg aaaaagagaa actctttttt taaccatgat aaacaatcct aaaaaaaacg
    36661 aacattataa gagaaaagct tttaacctcg tttctcctaa gctttagcag ggttaaacga
    36721 ttctcttaaa acattagctc ttttctttca gaaatagcgg ttttaaggta aaataatagt
    36781 aagttacctc tatgagatag ccagtaaaaa catatcgagg aaatatcaca tgattagaaa
    36841 gtatgaccgc acgagcacaa aaaagaaatc actaaactgg atttggctaa tcattgcttt
    36901 ttttatgatt tcaagcttta taggtggctc ttcttttact gagtcacttc tagacatttt
    36961 gcctgccata gccattggtg gtgcaggtta tgctattttt cgtgttagat ctcatcaaaa
    37021 gcggctagca aaggccaagg ttgccaaaca attagaagat ctaaaagcaa aaattcagct
    37081 agctgatcgt aaagttcggc tgttagatac ttatctggca gaccatgatg acttccaata
    37141 taacgtactt gctcagcaat tattaccaca acttagtgac atcaaagcaa aagctattac
    37201 gttaaaagac cagttagatc ccaaaattta tcgtcgtatc accaaaaaag ccaatgacgt
    37261 tgaaagtgat attacccttc aactcgaaac actgcagatt gccacaactc ttaaccctca
    37321 gcctttgaaa accccatctc ctaatcttat taacaaagca cctgagctca aaccgtatta
    37381 tgataatatt cagacagatc atcaagctat ccttgctaaa atccaaggag ccgacaatca
    37441 agaagaattg cttgcgctgc atgaagctaa catgagaagg tttgaggata tcttaacagg
    37501 ctacttaaaa atcaaagaag agcctaaaaa ttactataac gctgctgctc gtttagaaca
    37561 agccaaacaa gctatccaac aatttgatga agatcttgac gaaacccttc gtcgactcaa
    37621 tgaaagtgat cttaaagatt ttgatattag ccttcgaatc atgcaaggtg ctactcaaag
    37681 acgaacaacg catcaccaaa aagactaaag gagtcccttt atggcagatt ttaattttga
    37741 cattgaccaa attgctgaca atgctgttgt caaaaccgat aaaaccaccg atattatctc
    37801 agacttacca actgacacaa atggccaaat ctcttttttt gagaaactaa gtgctgacca
    37861 gcagacagct attactgcca aagccccggc attggtagat acctttttgg cagaccaaaa
    37921 tgcgctactt gattttggtc agtctgctgt tgaaggggtc aatgcaacgg ttaatcatat
    37981 cttggcagag caaaaaaaat tacagattcc acaagtggat gatcttttaa aatcaaccaa
    38041 tcgtgaactc aatggtttta tcgctaaata caaggatgcc acacctgtgg accttgataa
    38101 aaagccaaac tttcttcaaa aactctttaa acaaagccgt gataccctgc aagagtttta
    38161 ttttgattct caaaacattg agcaaaaaat ggatagtatg gctgcagcgg ttgtcaagca
    38221 agaagatacc ttagctcgca atattgtctc agcagaattg ttaattgaag ataatactaa
    38281 gtccattgaa catttggtag gcgtcattgc ttttatcgaa gctagtcaaa aagaagctag
    38341 tcagcgcgct gcggctttac aaaaagacct aaaaaccaaa gacagtgcta caccagacta
    38401 tcaaataaaa gctgatcttt tggcaagaac taccgaagtc atcaacactc tcgaacaaca
    38461 acacaccgaa tacttgagtc gcctctatgt tgcttgggcg acgactccgc aaatgcgtaa
    38521 tctagtcaag gtctcttctg acatgcgtca aaaactaggc atgttacgcc gcaataccat
    38581 tccaacgatg aaactatcta ttgctcagtt aggcatgatg caacaatcgg ttaaatctgg
    38641 aatgactgct gacgccattg tcaatgctaa taatgctgcc cttcagatgt tggcggaaac
    38701 cagcaaagaa gccatcccag cattagaaca atcagcacaa aaccctactt tgtctatgaa
    38761 atctgtgaca agtctggcag aaagtttagt agcacaaaat aatgggatca ttgcggcgat
    38821 tgatcatgga cgcaaagaac gagcacaact agaaagtgct attatcaggt ctgccgaaac
    38881 cattaacgat tctgtcaaac tacgtgacca aaatattgtg caagcacttc ttagtgaagg
    38941 caaggaaact caaaaaacaa ttgataaaac aactcacgca taagctaaac atcacctgtc
    39001 acgctactta aaaagcaagc catcagttag gttaatccct ttgatggctt gcttttttac
    39061 tacttaacga ataatcgcct tgtttgatat aacacttatc ttttatgatg ttattttcat
    39121 aatacgaaca aggctagcac gctcggcctc ttctaatttc aactcaatgt agtgaatcgt
    39181 ctcttccagt tgcgggataa tagaatactc taagccattg acgcgacgtc tagtcttttc
    39241 aatgtcatca gccattagtt gacaagtttt ttcaacttca gctaaccgta agagcttttc
    39301 taatagttct tttgtctttt ggatagtatt gtccatctca ctatttgagc tgaggtaact
    39361 ataagcaaac tcaccttgtt cttgctctct tgccatatta gactggacat ggaatttagg
    39421 cacattgaca ctcatgatat tttcaatatc aatccataag gtcacttcat ggactggaac
    39481 agcaaagagt tcttctacca ttagactatt ttcagatgct tttgccaaga caaactcctt
    39541 catattggca gcaagttctt tttcgatagt ttgccgaagc tcattgtttt cacgaattaa
    39601 atcaacgaag cggcgcatca actcatcccg cttgtccttt aataatttat gacctcttgt
    39661 agctgtcttc aatcgatttt tcaagttact aaattccatg cgtgttggtt taacatttaa
    39721 tcgtgccatt gatataactc ctttcgtcaa gggcttgaaa ttgcatggtc aactcaatca
    39781 tttgcaacaa aaacctttgt catcgtggta tctgctttag gcaaataacg atctaacata
    39841 tcatctttaa tgcgttttaa ttcggtacgt ggcaaaatcg acaacaattc ccaacccaag
    39901 tcaagacttt cttcaatgct acggttggtg taaaatcctt ggttaatgta ttcttcttca
    39961 aaccggttgg taaaggccac atatagttta tccgtttcag aaagggctga ttcaccgagc
    40021 acaacagcca attccttagc ttgttttcct tgggcatagg ctgcaaagag ctgattcatt
    40081 gtggcagcat ggtcttgtcg agtcttacct tcacctgagc ctttatcttt taggcgagat
    40141 agcgatggca agacattaat aggcggtcta aaaccatttt tatatagttc ttgcgacaaa
    40201 ataatctgac cttcggtaat ataacctgtc aaatcgggga tagggtgggt aatgtcatct
    40261 tctggcatgg ttaaaatagg aatttgagtc actgaccctt ttttcccgat cagacgacca
    40321 gcccgttcat ataaggtgga gagattagta tagagatagc caggataacc tcgtctgcct
    40381 ggaacttctc tgcgagcggc tgatacttct cgtaaggctt cacaataatt tgtcatatct
    40441 gtcatgatga ctaaaacatg catacctttt tcataagcca agtattcggc tgttgtcaga
    40501 gctatacgtg gtgttgcaat gcgttcaata gcagggtcat tagccaaatt gataaataaa
    40561 acagatcgat caatggcacc cgtttcacgc aagtcattca taaagaattc tgcctcttca
    40621 aaagtaatac ccatagctgc aaacacaacc gcaaagttat catcagagtt tagaacagtt
    40681 gcctgacgag caatctgtgc ggccaattca ttatgaggta accctgagcc cgaaaaaact
    40741 ggtaactttt gaccacgaac gagggtattg aggtgatcaa tagctgagat acctgtctgg
    40801 ataaattcgt ctgggtaatc acgagcgaca ggattgatcg cttgaccatc gatatcaagg
    40861 tatttttcgg gaataagttc tggaccgccg tctatgggct gccccattcc atcaaagatg
    40921 cgccctacca tatcttctga tacggctaat tccaaaggat gccctgtaaa tcgaaccctg
    40981 gttttggcta ggttgatgcc agttgatcct tcaaatagct gtaccatagc cttgtcacct
    41041 tggacttcta agacttgacc tttacgcctt tctccattat gcaaagtaat atctaccaat
    41101 tcgttgtagt gcactcctgc aacttgatca acaatcatca aagggccaac cacttcgcta
    41161 accgtccgat attctttgag aacgctcatc taaattcctc cctttgctaa aacgtggtga
    41221 atctctttgg taaccttctg agtaagccct ttaatctgct ctaagttttc ttctgggata
    41281 aatttgctgc gtgcgatacg atcgcgtacc tgagcagtgc cttccataat ctcattaaaa
    41341 taagctccca aagaaagggc tttgctggct tcctcattaa aggtcaaaat attggttagc
    41401 atggcctctt gtttcggaaa ggaagtaaag gtatccaccg aatcaaaggc attttgctgg
    41461 agataatcct cccgaatttg ccgggcaaca gccatggtca aacgatcttg ttcagacagt
    41521 gaatcaagcc ccaccaagcg tacaatttct tctagactgg cttcacgctg caaaatagcc
    41581 atggcacgag ttaccttgtt ggaccaatta gattcctgtt tacggtcaat atagcttcct
    41641 acatcatctt gatacaatga ataagacgtc agccagttaa tcgctgggaa gtgacgccgt
    41701 tgcgcaagag gcgcgtcgag cccccagaaa actttgacaa tccgaagggt gttttgagtg
    41761 acaggctctg aaatatcccc tccaggagga gaaaccgcgc cgatggctgt aatggttccc
    41821 tcacgttctt gacttcccaa ggtccgaaca cgaccagccc gttcataata ttcggcaata
    41881 cgactcccta agtaagccgg atagccttca tcaccaggca tttcttgtag gcgtcctgac
    41941 atctcgcgca gagcttctgc ccaacgtgat gtcgagtctg ccatgatagc cacagaatag
    42001 cccatatcac ggaaatattc ggcaatggta atgcctgtgt aaatcgacgc ttcacgcgct
    42061 gctactggca tattagaggt gtttgcaatt aacaccgtgc gctccataat ggactgacct
    42121 gtatttgggt caattaactc tggaaactca ttcaaaacgt cggtcatctc gttgccgcgt
    42181 tccccacaac cgacataaat aacaatatca acgttggcaa atttagctat ttgatgctgc
    42241 acaactgttt ttcctgcccc aaatggtcca ggaacagcag cggcaccacc ttttgtaaca
    42301 gggaaaaagg tgtcaataac ccgttgacct gtaaccaaag gttcgactgg gataagcttt
    42361 tgagcaacag ggcgactttg acgaactggc caagtctgca tgaggctacc ttggtaaatg
    42421 gaaccgtctt ccttcttaat ttcataaact gtatctgtta ctgtgaagtc ccctgcatga
    42481 atagccacca aggtccctga aactttataa ggaaccatga tacgatgctc aatgacagct
    42541 gtttcttgca cagttcctaa aatatcacct gcaactactt cttgaccaac acttagcttg
    42601 ggaataaatg cccacttagc ctttcgatca agacttggga tagccacacc acggattaaa
    42661 aagtcgctgt ccgttgcttt ttgaaaacga tcaagcggtc gctgaatacc gtcaaacatt
    42721 tctgaaatca ggcccgggcc taactcgact gacaaaggac aaccagtagt cactactggt
    42781 tctcctggac cgatccctga tgtttcctca taaacctgaa tagacgcttg atcgcggcgc
    42841 atttcaataa tttctccgac taagccaaga tgtcccacac gacaaatatc ttgaatatta
    42901 gcttcttgca tcccagaagc cacaacaaga ggtcccgaaa cggttattat ttttccttgg
    42961 ttcacctttt tcctccttta ttttttccaa atcatactaa ctaatctata ctgtcaaggt
    43021 ttaatgtgac tattttgaat agtcagcaaa ctatcctatg cgttcagctg ctaacttcac
    43081 caattttcct gtctttgcat ttcatcagtt agctactcat aagcctcaaa cagagtataa
    43141 gtcctctttg cataacgtct tagtgttata aaatattttg accgacagcc ttttcaacca
    43201 tggcttgtat gcgtgacagg cctatccctt gcgctccttg atgagttggt aatggtatga
    43261 ctgctggtag aacttgattg tcatagtgag tgagggcttc tggaatagct gcagcaatat
    43321 cttctgtgat ataaataata ccaaagtctt ccattgctag ttgacgcaac tggttaatag
    43381 cgtcttgtgg ttttattact ggaaaagttt gaaacccaat catctgaaaa ggtagaataa
    43441 catctctgtt accaattacc ccgactttat acaatgtttt ggtcatgcta ttggtctcat
    43501 cctttctatg acacgttctt tgggtaaatc attagctaaa gcagccgcta aaagccgtag
    43561 attttttact tcaaacgatt tagcgagcaa aaaccgagca agcacataag gcccatcgac
    43621 ttgatatttg gccttcgcaa ataaatggta aaggcattca tcaacaaggt attctaactc
    43681 agtcgtttga agagtgcctt ggcgaagttt ctctgaataa ggttttaggg ctgtcatata
    43741 gctatcaggt gttacatttt caaaccacgt catcagatcc ttatcatctt caagtgtgat
    43801 aaatgtttta cttggtctac ttgcttcatc agataataac tgcatcatga aacttattgg
    43861 tttattttgc gacttggccc gtctgacggt aatcagatta tacaaatcaa tcacaataag
    43921 aacagcttgt tggaaaacag ggtcctctaa tctttgtgct atttgtttca acgccttaaa
    43981 ataagctaag tcagtaccaa tttctaagac gcgtatgtct tgataatctt gatattcggc
    44041 ccaaatggac tggatttctg ttaccacctc ttttggaaac tcatcagaag tcatcgtcct
    44101 aatcaaatgt tccaaagcca ccaaaggctt gtcaccaatt ggtagtaata agtgattcag
    44161 atctgcttga ctagctttag cttttaacaa cactttgaca ttatgatagg tgtaacgcaa
    44221 ggtaaataat tgtactatgt caggttgagg ggtttctgca aaagcccagc gataggtctt
    44281 ggttagctca gccattaaga tagattctag ccgatccaaa tcgttgagat cgtcaacagt
    44341 taggtgatag actgactggt gcaacagtcg agctaaggtt gttgtattgg gagcctgcaa
    44401 tagcttatca aattgctctt tagtcaataa ctctttttct ttgacactga tagtagtgtt
    44461 taattctaga aatgttggca tcgctagtca aacctcctat atctctgaaa aaagcatatc
    44521 aatgatgcgg gagctttctt ctttaagaac tgattctaaa aggtaacggt ataaataatt
    44581 gtcatcgact tggtctaaac tgactaaaaa tcctgcttcc tgaggaatta gctcttgatt
    44641 atagcttaat tgaggaaatg ctaatctcag ctcagcacaa tcataagaac tgaacttttg
    44701 ccctgttttt tcgccaaaag taacttgcaa tggctgttct ggatatttag acaagacttg
    44761 cttcaaaaaa gccaattctt cttccttaga aaagttcgtc atcttctcca aagaagcttc
    44821 aaatagctcc tttatactgt cttgttttaa tgctagtaga gcttgtcttt cttgattttt
    44881 gagttgctgc tgcgcaacct gaaattgctg ttcaagagct tttaactctt tttgacgctt
    44941 tgcttttaaa tcgtgtagtc cttgctgttg acgctcttta aagtcagtat caagactatc
    45001 tgtcgctatc tttaggcact gttgaccttc ttgatgtgct ttttcaagaa cgttttgtct
    45061 taactgtgtt atatcattca cctaatttcc cccttttgat agtgttagtg gtgccaccat
    45121 tttttaaaat ctagataagg accatacctt atccaatttt atttaagaga ataaaggaga
    45181 cgacaaaggc aagaatagca taggtttcaa ccatagctgc taaaatgaca cccttcataa
    45241 aatctttagg gcgcttagcc aagatttgca taccagctac agaaacattg ccttggtgct
    45301 tggccgaaaa ataaccaaca atagcaattg gaagagatac caaaaagtaa gctaatcctt
    45361 ggttaactga aagttctggt gtcagtttca tccaaattaa aataccaata gcaaagccgt
    45421 agattccttg actaccgggt aacagttgca agataagcgc agacgtaaat ttttcgggtt
    45481 cttcttttaa gagcgcagct gccgcttgac cacctttacc gacaccataa gctgagccca
    45541 tgccactaag agctacggct aaaacaattc ctaaggctgc gaagaaaacg ccaccatgtg
    45601 ctgtaaaata atgtgctaaa tgttccatgt taattgtcct ccaaatctgt ttcttcattg
    45661 acattgacat aattatcagc taactttaaa ggattgaaag cttttcctcc accttcataa
    45721 aattttccaa aaaactctac gaaaataaga cgagcaccat gcacatatcc tgaaagcatt
    45781 gataagaaaa tattgatcgc atacaaaaga ataaaaataa agatacctac tgtaaagcgc
    45841 gtgactggtg gaaaaatccc aacaatcatg ttaaaagccg ctccaatact agctccagaa
    45901 agccctaatg ccattagacg agtaaagcta accaaatcac ttaagtaaga gctaatgcca
    45961 tataaattat ataatccgct acctagacct aaaaggcttt tgctttttag taaagaaaca
    46021 accaaaatcc caaaggcatt gcccaatgca agccacttac caataaccga taagtaagca
    46081 aggccagata cattcttacc aagaataaat aacagcaggc ctaataaaat aaaagtccaa
    46141 gctagacttg acgtataggc cgtagcatat gctttcatcc ttacttgttg ccatgctcct
    46201 aacaaaagcc caaatataag tgtaacaaac ccaaatagca aagaaacaac caaaatagta
    46261 atcacatccg ttttagttga taataatgcc acaggtaagt caaaaccaaa gaaagaaccg
    46321 taaataagcc cccatatggc tactgaaata gctaaaatat taaagaaggt cactaaccgt
    46381 ttactggttt tttggagatt aaaaaagacc aatgcagcta gtgtcacagc atatagcaac
    46441 aaaccatagc ccaaatcagc taccatcatg ccaaaaaaag taaggtataa aggtgctaaa
    46501 aaaggtgtcg gatctttttc ttgatatttt ggcaaagcgt acatttctgt aaccaattca
    46561 aacggtgcaa tgtagcggtg attacgtagc ttaatgggaa catcttccca atcgtctggc
    46621 gtgacatcgt aactatccag ataaaacata tcacctagtg tcttagtcat tagccctttt
    46681 aattggttaa ccgaatcagc ttctatccac ccttctaagg ctattagata ttttgtaccg
    46741 agtaattgct ttttagtttg ctgccgttga tactgactaa ggacatagtc tatttcaacc
    46801 tgtaactgtg ccagtatctt ttgagactgc ctaagctctt ttagtagact atctttttca
    46861 gctagccaat tcgttagtaa ctctttgttt atacgaagtt gttctgatgg cagtagctgt
    46921 tctttatata ataagggttt aaactgatat ttctctaaat gaatcgtatc ttgtgcttgc
    46981 caaaataaga caaggccgta ctcaaattct gtatggtata cttcctcaat aaaaagacct
    47041 ttgtgctgct ttaactgatg ataaaaatga tcatttgctg tactaggaat cgttccaact
    47101 cttgccgcca aaaaatgaaa ttgcgctaag tcctgaggca aaacagtcaa ggaacgccac
    47161 ttttctaact cctgacagta ttctgttagc tggtctattt tgttttgcag ctgctctagt
    47221 cgctctatct tttgtgacat cattttcaaa gccgtttgag ctgcttgttg tcgatggcgt
    47281 tcttcgaact gtttaaacga aagagatggc gttttttgtc tcaaggctgt taactttcct
    47341 attgatggta aaaacgctga taagctagca aggccgtttt ctaactcttg ttggctttgt
    47401 aaaaggtatt gtaaagcttc atcatctagt agaggttttt gatgcgtcag atcgtattgg
    47461 acgatttgtg gcaacttgac gttgcctttg ttaaaagcat cttgccaatg ttttagttgc
    47521 ttcatgtcac gaacttcaac taattgagat tgttgaagtg tttttaaaac aagatcaaga
    47581 taatcttttt caaaaaccat tgccagcttt ttcatttgac taatggccat atgtctccac
    47641 caccttatcc actaactccc taacaacggt tagcttatgc tcttctaaaa tggcatgaag
    47701 ggctagctgt tgcgtcgcta acctcttttt tgaagctttt tgttcttgac gattagcttc
    47761 aaaaaacacc tcacgtttag cttctaacag tagctgagtt tcttgttcgt actcaaggaa
    47821 cagctgcttt aactcttctt cttttttgtc agctagagct tttttcttag catcgtaact
    47881 gtcataaatt gttctagcct cttgttcgat actattcatt agtgacaata gggtattcat
    47941 cacatatcaa ctccttatct ttatctatat gctaattctt tgactcacta ggtacttaaa
    48001 aacaaagcta caagaaaggc tacttttggg ggttaatcaa ccagcgaata taagctagag
    48061 ctaaatctct aacctatcta ctaacagctt ttttgagata ggataattgc ttgattaagc
    48121 gaacagtatc tttaagttaa gcgctttcaa ttctatccat aaaaacaatg gcttagttca
    48181 gaaaaaggtt agctaaaaac ctgtcaaagc agtaagatga cacagaactc taaagctttg
    48241 tattgctgtc tttcgtgtgt tatcttatac ttctttaggc atttagtaac cttgatctag
    48301 ttgacggtaa ggaaccttta ccagccaata gccaagccaa tgtgataaca gtataactgc
    48361 tatcttgtta aaagtcctgt caaaaagatg tttattagtt tctcgcttgt aattcttgtt
    48421 ttgttactaa acggtaattt ttaaagacag aacctccaat ataacccaca attaagctga
    48481 taataataca tccaagggct gttatcaaca ctttagcgac aggattatag gcaaacatta
    48541 ctgcaaaacc ggcaatcgga gtagctgtcc ctggcgtatc attaaccaat ttcattaggg
    48601 cgatcaaaac accacatgca gcaccaccaa caaaattagt cacataaatc ggaatgggat
    48661 tggctgacgt cacatctgcc tgcgttagtg gttcaatagc aaaagcaata ttgtcttttc
    48721 tttcacccaa ttttaatcgg taaaaaagaa caccattcat aaaagaagag ccaaagacag
    48781 ataaggcacc tatagccatc ggaatacctg ttagaccaag catggctgtc aaagccattg
    48841 atgacagcgg agctgttgct acaacaacaa tcgtaccgcc tagtatcatt cccatgataa
    48901 tagggttgct attagcactt gaggtcagga tgtctccaat agtcaataaa gtactattga
    48961 tcactggcgt aattaactgc gccaagaaac gagttaatgg tgcaccgaca ataataatcg
    49021 agattaaatc aagcccacca ggaatatttt tttccatcca cttgattaaa aaagatacca
    49081 catacccagc cacaaaacct gggataatac ccgttcctga aactgacaaa ccaatcaaaa
    49141 cggcataacc tggagacaca ccaattgcaa tggcaaccaa gatagcagca gctacccctg
    49201 ataatgagcc atttgctccg cctacttcac ttagaaactg aaagcctaaa accttaccaa
    49261 aaaaagaatc ttgaaaggct tcaactaaaa aagaggcaca agctgccgaa gccaaagctc
    49321 ccattgcctt ggcaccatga ggagccttat agttaaacaa gctaaaaata gctaaaacaa
    49381 gaatcaaaag acttgttcca ataataatat ccataatcta ttccaccaat ctatctcatt
    49441 acataagaca ctataactgc ttcactctgg cgacagcttc gacttcaatc aaagcttgca
    49501 aaggaagcgc cgacacttga accgctgtcc gaacagggta gggctcttca aacaaatcag
    49561 tcatgacatg atttaatatt tccacattgg tcacatctgt taaatacaca tttagcttat
    49621 agatatgatt caagtccaac ttttgctctg ccaaaataga ttgtaaatta acaaaaacct
    49681 ggcgacactg tgcctcaaaa ccatctgaca attgacctgt cactggatta aggggtaatt
    49741 ggcctgcggt gtatagaaaa tgtccttcaa tcgtataagt tgaataaggg cccattggtt
    49801 ctgggtagct cttcatatac gaacctcctc attgataata tagttaaatt ttacaggaaa
    49861 ccgcattcac agtcaataat aaattctctt tttgataaaa aattattatt aaggtgattg
    49921 gcaattgttt tttagtctaa aatgtattac tatatttgta aataagaaga tagatatagg
    49981 aagtttgaca tggataaaga aacgctaaac tactggaaaa cggttatcac atttcttcac
    50041 gatgtcttag gtgataacta tgaaattatt ttacatgtga ttgataaaaa tgatatctat
    50101 attggagaac tcgttaatag tcatatcagt ggacggtcta aacagtctcc tctcaccacc
    50161 tttgctcttg atttgattac taataaagtc tataaggaaa aagattttgt gaccaattac
    50221 aaagcaatcg ttagtcctca gcacaaagaa gttcggggat ctactttttt tattaaagat
    50281 aaaaagggta atctcgaggg aatgctatgt attaacttag atatttcagc ctatcaagga
    50341 gtagctaggg acctcttaaa acttgtcaat ctcaatttag aacatttcat cccaactgct
    50401 aaagagccta aaacagtcac accccagcca gaagaagctg ttgaaatttt aacctctaac
    50461 atccaagata ttatcggtca aattattgat ccctcgttac tgagacacaa tgtacatctt
    50521 agccaagatg tcaaaataga cattgttgcc aagctttatg aaaaaggggt ctttcagcta
    50581 aaaggagccg tctcaaaagt cgctgatatt ctatgtattt ccgaaccaag tgtttaccgc
    50641 taccttaaaa aaattgaagc cgatcaataa ccaaatcacc ataaaacgcc agttaaaagt
    50701 atttcagata agcatcacaa tctctgatat accgatgact gacgtttttt ttactcaata
    50761 tgcctttatg gcaagaaaag gctactggtc attaaccttc agctatgctt agaggtacac
    50821 ctgtcgcagc ttgaacctca tcaaaagtaa acccttcact gatttcaatt acttggatac
    50881 catctggcgt tactttaaac acacccattt ctgtgataat aaggtcaacc acaccttttg
    50941 ctgttaaagg taaggtacat tcttttagga gcttatgctt tcctttattg gtatgctcca
    51001 tagctacgat gactttttta gcaccaacta ataaatccat cgctcctccc ataccaggaa
    51061 ccattttgcc tggaatcagg taattggcga tatctccatt ttctgcaatt tctaaagcac
    51121 ccaagacggt caaatcaaca tgaccaccac gtataatgcc aaaagaatct gcactattaa
    51181 agaaagcggc tcctggataa accgtcacag gctgtcctcc tgcattaacg atagtcgaat
    51241 tcggatcatc tgacttagga cctagtccaa taaacccatt ttcagattga aaatacactg
    51301 tcatttcttc aggcacaaaa tttgctactt tggttggtag accaatacct aaatttacca
    51361 aagtaccatc ttctaattct ttggccacac gctttgcaat acgattttga atcacttctt
    51421 tacttaatgc aaccgccata actatactcc ttctttaaca atgtaatcta caaaaatgtg
    51481 tggtgtatga acgcattctg gttgtatggt gccgactggt actatttctc gcgcctgaac
    51541 aattgttgtt tttgcacaag ctgccatcat accaagaccc cagtgtaaaa agccaaagat
    51601 agagcctact acaatagaaa aaataaaaat ctgagtcctg gtttgaggca gtttggcagg
    51661 ggacttaaac attttatgag caggtggaga actcgctaca gcattccctg tcgcaagtaa
    51721 aatagtcatc tgcatggcaa aggacaacag cccccaaaaa ccatttaccc agccatagat
    51781 aatcccgccc gtcttttccg taccaataaa gggactatcc gtcatcaaca cagacatagt
    51841 aaggactaaa aaggttaaaa taaaacagat aaaaagcgat tctggcatcc atttcataaa
    51901 gccatcaata tagcgatcca taaacctttt tttggttttg atgttattca tgttgtcaat
    51961 tcctttcaca ttgtccacaa gcacttttgt tcttttgatc agtaacctat cgataatact
    52021 aataagcgtt tctttagttc atttagttat caataagttt aaacccacaa aaaacacttt
    52081 tgttcccttt ttattatgac aacgttttca taaacaaaca actatgattt ttttattgct
    52141 tccataaccc ttatttata
//
LOCUS       DAUCWU010000007        47962 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUCWU010000007 DAUCWU010000000
VERSION     DAUCWU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47962
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.007"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            273..1832
                     /locus_tag="VGY24_000382"
     CDS             273..1832
                     /locus_tag="VGY24_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ4283393.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKESHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQI
                     TTKSFIVYQKK"
     gene            1929..4127
                     /gene="nrdD"
                     /locus_tag="VGY24_000383"
     CDS             1929..4127
                     /gene="nrdD"
                     /locus_tag="VGY24_000383"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ4283394.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIGLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            4309..4455
                     /locus_tag="VGY24_000384"
     CDS             4309..4455
                     /locus_tag="VGY24_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283395.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            4484..5419
                     /locus_tag="VGY24_000385"
     CDS             4484..5419
                     /locus_tag="VGY24_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ4283396.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSISSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            5428..5937
                     /locus_tag="VGY24_000386"
     CDS             5428..5937
                     /locus_tag="VGY24_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4283397.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGILEDSRGGTERY
                     WIEEQRGDK"
     gene            5937..6551
                     /gene="nrdG"
                     /locus_tag="VGY24_000387"
     CDS             5937..6551
                     /gene="nrdG"
                     /locus_tag="VGY24_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ4283398.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRCELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(6725..7456)
                     /gene="yaaA"
                     /locus_tag="VGY24_000388"
     CDS             complement(6725..7456)
                     /gene="yaaA"
                     /locus_tag="VGY24_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ4283399.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(7498..8355)
                     /locus_tag="VGY24_000389"
     CDS             complement(7498..8355)
                     /locus_tag="VGY24_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4283400.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLVLNHFLTRGISLEGAAD
                     HGYSEAIYLSDPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            8542..8912
                     /locus_tag="VGY24_000390"
                     /pseudo
     CDS             8542..8912
                     /locus_tag="VGY24_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9158..9871)
                     /gene="treR"
                     /locus_tag="VGY24_000391"
     CDS             complement(9158..9871)
                     /gene="treR"
                     /locus_tag="VGY24_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ4283401.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            10082..12106
                     /gene="treP"
                     /locus_tag="VGY24_000392"
     CDS             10082..12106
                     /gene="treP"
                     /locus_tag="VGY24_000392"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ4283402.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLAETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGVLLQPTEGVLVAP
                     CDAEVSVLFPTKHAIGLVTAEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            12173..13801
                     /gene="treC"
                     /locus_tag="VGY24_000393"
     CDS             12173..13801
                     /gene="treC"
                     /locus_tag="VGY24_000393"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ4283403.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMGQGNGWNALFYNNHDQPRALNRFIDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAK
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILAEN"
     gene            14009..15904
                     /gene="pepO"
                     /locus_tag="VGY24_000394"
     CDS             14009..15904
                     /gene="pepO"
                     /locus_tag="VGY24_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ4283404.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHVPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIAEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(16071..17111)
                     /locus_tag="VGY24_000395"
     CDS             complement(16071..17111)
                     /locus_tag="VGY24_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ4283405.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(17245..18012)
                     /gene="rpsB"
                     /locus_tag="VGY24_000396"
     CDS             complement(17245..18012)
                     /gene="rpsB"
                     /locus_tag="VGY24_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ4283406.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADIAFEADTQADSIEEIVEVVEGDNA"
     gene            18316..21390
                     /locus_tag="VGY24_000397"
     CDS             18316..21390
                     /locus_tag="VGY24_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4283407.1"
                     /translation="MKKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTAEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTS
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVTY
                     QKQAIALTENKPAELSASIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(21421..22407)
                     /locus_tag="VGY24_000398"
     CDS             complement(21421..22407)
                     /locus_tag="VGY24_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEQ4283408.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(22593..24134)
                     /gene="hutH"
                     /locus_tag="VGY24_000399"
     CDS             complement(22593..24134)
                     /gene="hutH"
                     /locus_tag="VGY24_000399"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ4283409.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACHIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAVGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(24145..25488)
                     /locus_tag="VGY24_000400"
     CDS             complement(24145..25488)
                     /locus_tag="VGY24_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ4283410.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFESLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(25708..26304)
                     /locus_tag="VGY24_000401"
     CDS             complement(25708..26304)
                     /locus_tag="VGY24_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ4283411.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     TATVALRQTTFTRLDGYHRILMSLDKPITLSDLSHAKEISLAPFQPYAFEGDTRIVSR
                     GTCQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVTEQEASLSDLKVCLNQTSPAQTPVAVWAGLWYKT"
     gene            complement(26326..27999)
                     /locus_tag="VGY24_000402"
     CDS             complement(26326..27999)
                     /locus_tag="VGY24_000402"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ4283412.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLT
                     KIVTKVYGGKGINLSSAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(28017..28643)
                     /locus_tag="VGY24_000403"
     CDS             complement(28017..28643)
                     /locus_tag="VGY24_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formiminotetrahydrofolate cyclodeaminase"
                     /protein_id="HEQ4283413.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQDIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(28654..29553)
                     /gene="ftcD"
                     /locus_tag="VGY24_000404"
     CDS             complement(28654..29553)
                     /gene="ftcD"
                     /locus_tag="VGY24_000404"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ4283414.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(29641..31671)
                     /locus_tag="VGY24_000405"
     CDS             complement(29641..31671)
                     /locus_tag="VGY24_000405"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ4283415.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFEEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRFRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIKTRHSQGWISQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGITFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            31963..33228
                     /locus_tag="VGY24_000406"
     CDS             31963..33228
                     /locus_tag="VGY24_000406"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ4283416.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGTQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(33286..34818)
                     /gene="ahpF"
                     /locus_tag="VGY24_000407"
     CDS             complement(33286..34818)
                     /gene="ahpF"
                     /locus_tag="VGY24_000407"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ4283417.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLNYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(34839..35399)
                     /gene="ahpC"
                     /locus_tag="VGY24_000408"
     CDS             complement(34839..35399)
                     /gene="ahpC"
                     /locus_tag="VGY24_000408"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ4283418.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            35805..35875
                     /locus_tag="VGY24_000409"
     tRNA            35805..35875
                     /locus_tag="VGY24_000409"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35837..35839,aa:Cys,seq:gca)
     gene            36395..36598
                     /locus_tag="VGY24_000410"
     CDS             36395..36598
                     /locus_tag="VGY24_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ4283419.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            36794..37255
                     /locus_tag="VGY24_000411"
     CDS             36794..37255
                     /locus_tag="VGY24_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ4283420.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            37255..39699
                     /locus_tag="VGY24_000412"
     CDS             37255..39699
                     /locus_tag="VGY24_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4283421.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDDAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            39877..40167
                     /gene="groES"
                     /locus_tag="VGY24_000413"
     CDS             39877..40167
                     /gene="groES"
                     /locus_tag="VGY24_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ4283422.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            40203..41834
                     /gene="groL"
                     /locus_tag="VGY24_000414"
     CDS             40203..41834
                     /gene="groL"
                     /locus_tag="VGY24_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ4283423.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAVAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            42045..42398
                     /locus_tag="VGY24_000415"
     CDS             42045..42398
                     /locus_tag="VGY24_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4283424.1"
                     /translation="MKSYSHIWVLEGNFGEIFKTFRESKGLRLKDVAKAGVSTSQLSR
                     FEKGETDLTISTFMLILDESNMPINEFMYAVHDFHRDNTIQDSDGLKQLLHAQLASTD
                     KKEMFTDIFWHIQHV"
     gene            complement(43026..44522)
                     /locus_tag="VGY24_000416"
     CDS             complement(43026..44522)
                     /locus_tag="VGY24_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ4283425.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGVMWLSIGSPRNAPYLPYLGNISRTYEAYQ
                     EKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKTSKEISDL
                     VVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVKEKPKNKKGLNRS"
     gene            44769..46409
                     /locus_tag="VGY24_000417"
     CDS             44769..46409
                     /locus_tag="VGY24_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283426.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGLKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(46498..47499)
                     /locus_tag="VGY24_000418"
     CDS             complement(46498..47499)
                     /locus_tag="VGY24_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4283427.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNGPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTLEDICVQAKSATFDAVLNAQKARK"
BASE COUNT        14056 a         8997 c        10507 g        14402 t
ORIGIN      
        1 agatttagag aattattaca gtatgtgaaa atatatcaga aattgactaa ataaaagatt
       61 ctgttatact tgttttataa tattctttga cgataatatc atttttcaac ttactttgtt
      121 tatacactgt cactttgcta tgtgcaatac aaagtcatgt tatttatctc atactaaaaa
      181 ataatggata atagctatgt tagactagtg atatgaagaa atattgcttt tattgaatcg
      241 ttagagaata tgatatttca aggagaaaaa tagtgatcga gttaaacgat gaattcatcc
      301 gaaaagagac gatagaactg gccaacgatg gcccacgagt acacaccact caatatgaaa
      361 ctaaagtacc tcggcttcat aaatgctatc tgcttttctt tagcattatt atcagttctt
      421 taacaattgc agtacctttt ttaacagatg cagccaatgg tcttcagtcc caaaacttat
      481 atataggaat gatgttaacg aaggggcaac ttccctatag tgatgctttt acaacaggag
      541 ggctctttta ctttgttatc attgcattaa gttattattt gggatcgaca ctttggctag
      601 tctttgttca ggtgttttgt ttttacttat ctggtttata tctttataaa ctcattaatt
      661 atatgacagg ctttcaaaaa gtggctttaa ctttttcaat tagctactat ttgttatctg
      721 ttagtcttgg ttttggggga ttgtatccta ctcaactggc tatgccattt atattaatat
      781 cggcttggtt tttaactaag tattttgcct gtttagtgaa agatgaggca tttattcttt
      841 ttggctttgt aggtgctctt gcaatgctaa ttgacccgag tacccttatc ttttggtctt
      901 ttgcttgtgt gacagttttt tcttataata taagccaaaa gcatcttgca agaggttttt
      961 atcaactgct agcttcgatt tttggaatga ttttagtttt ttacacagca ggatatttca
     1021 ttttgaactt acaagtgcta aatccttatt tatcacaaac gatgatttat ccttttactt
     1081 tttttaaatc aggaaactta tcgttgcttt ttggactggc tattcagttg ttcttcgctt
     1141 tggggcttgg tcttttgacg ggaatggaga atgtcattag gcgatttaaa aacaattctg
     1201 atagggtcgt caagtggcta tttgtcatgg tcattctaga atctatactt gtggctatat
     1261 tttcacaaga ctatcgcccc tatcatcttt tacctctttt accttttgga ttaattttga
     1321 ctgctattcc tgttggctat cagtatggta taggattagg tcagagtagt catcgcagac
     1381 gtcatggtaa aaatggtgtt ggtcgagtaa tgatgattta tcttaagaga cacttttatt
     1441 tgccaatttt aattgtaggg acaatactaa tctgttctac ttattgtttc attagtagta
     1501 ttcctcttaa tcaggagcgt gatcatattg ctagttattt agaacagaaa ctaaataaaa
     1561 ctcaatctat ttatgtttgg gatgatactt ctaaaattta tttggacagt aaagctaaat
     1621 ctgtttctca atttagttct cctgacatca atacgcaaaa agagagtcat cgaaaaatat
     1681 tagaagatga actattagaa aataaggctg cttatatcgt tgttaatcgc tataaaaacc
     1741 tgcctaaaat cattcaaaaa gtattatcta ctaattacaa agtagataaa cagataacga
     1801 caaaaagttt tattgtttat cagaaaaagt aatctaatat caatatattg tgttcgagca
     1861 aaaaacttga cacaatatat tgattttttt agttgtcgtg atataatagt tccgtaagga
     1921 gatttgatat ggtaagttta gaagaagaca aggtgactgt tcaacctgat attaaagtga
     1981 ttaaacgaga tggtcgcctt gttaattttg atagtacaaa aatctatagt gctttattaa
     2041 aagcaagcat gaaagtaact cggatgtcgc cacttgttga ggctaaatta gaggctattt
     2101 ctgatcgcat tatagcagaa attattgagc gttttccaac taatatcaaa atttatgaaa
     2161 tccaaaatat tgtagagcat aagcttcttg cagctaatga atatgctatt gcaaaagaat
     2221 acattaatta tcgtactcag cgtgactttg cacgttcaca agcaacagat atcaattttt
     2281 ctattgataa attaattaat aaagatcaaa cagttgttaa tgaaaatgct aacaaagata
     2341 gcgatgtttt taatactcaa cgagatttaa ctgctggaat cgtagggaaa tcgattggtt
     2401 taaaaatgtt accttcgcat gttgctaatg ctcatcaaaa aggagatatc cattaccatg
     2461 atttggatta cagtccttat acaccgatga cgaactgctg tttaattgac tttaagggca
     2521 tgttagccaa tggctttaaa attggtaatg ctgaagtgga aagtcccaag tctattcaaa
     2581 ctgcaacagc tcagatctca cagattattg cgaatgtagc atcaagtcag tacggcggat
     2641 gcacagctga tcgcattgac gagtttttag ccccatatgc ggagcttaac tttaaaaaac
     2701 atatggctga tgctaagaaa tggatcgttg agactaagag agaaagctat gcttttgaaa
     2761 agactcaaaa agatatttat gatgcgatgc agtctttgga gtatgaaatt aatacgctct
     2821 ttacgtctaa tggtcaaaca ccatttactt ctttaggatt tggtttgggg acgtcttggt
     2881 ttgaacgtga gattcaaaaa gctattttga ccattcggat taatggtctt ggtagtgaac
     2941 atcgcacggc tattttccct aaattaattt tcacggttaa acgtggcttg aatttagaac
     3001 cagattcacc aaactatgat attaagactt tggctttaga atgtgcgact aagcggatgt
     3061 acccggatat gttatcttat gataaaatta ttggtttgac aggatctttc aaatctccaa
     3121 tgggatgccg ctctttcctt caaggctgga aagatgaaaa tgggcaagat gtgacctcag
     3181 gccgtatgaa tcttggggtt gtcaccctca atttacctcg cattgccatg gaatcaaatg
     3241 gcgatatgga taagttttgg gagctgttta atgagaggat gctaattagt aaggatgctt
     3301 taatttatcg tgtcgaacgt gtcacagaag caaaaccagc aaatgcccct attctttatc
     3361 aatatggtgc ttttggaaag cgtttggaga agacagggaa tgtaaatgat ctctttaaga
     3421 atcgtcgtgc aacagtctct cttggctata ttggtcttta tgaagtggcg tctgtttttt
     3481 atggtggtca atgggaaggt aatccagatg ctaaagcttt taccttgtca attgtcaagg
     3541 caatgaaaca ggcctgtgag gattggtcag atgaatatgg ttatcatttc tctgtttatt
     3601 cgactccatc agaaagtttg acagatcgct tttgtcgttt agatacggaa aaatttggca
     3661 ttgtgacaga tattacggat aaagaatact atacaaattc ttttcactat gatgtgcgta
     3721 agagtccgac accttttgaa aaattagatt ttgaaaaaga ttatccagaa gcaggtgctt
     3781 caggtggttt tatccactac tgtgagtatc ctgttttgca acaaaatcca aaggccttgg
     3841 aagcggtttg ggactatgct tatgatcgtg tggggtattt gggaaccaat acgcctattg
     3901 ataaatgcta taattgccaa tttgaaggcg attttacccc aacagaacgt ggttttactt
     3961 gcccaaactg tggcaataat gaccctaaaa cagttgatgt ggtcaaacgt acatgtggtt
     4021 acttggggaa tcctcaggcc cgcccaatgg ttaacggtcg ccataaggaa atctctgcgc
     4081 gtgtaaaaca tatgaatggt tctactataa aatacccagg cctgtaaact caagctgggg
     4141 gagagccctt ttgatggaaa tatgatatgc ttaaaaggtt gcttcatgca agcaatcttt
     4201 tattgtttaa tggtttctta gcaattgtga gataagaata actaatccaa aacgactaat
     4261 tagctgttag caatcggact ataagaagaa agagataagg aagaaataat gggaaaatat
     4321 caattagact ataaaggaat gcagcaggta gaacgctttc atgagaagca ttctaaaaaa
     4381 aagacggata aaaaatcccg cgttcaagaa cttaaggctc ggtttttaga gaagtcaaaa
     4441 aaacaggata actaaagtat ccagtaaaga aagaaggttg agtatgctta atattgggat
     4501 tgtgggacta ggtgctattt cacaaaaagc ctatttacca tatatgagac agctgagtga
     4561 cattacttgg catctatcaa cgcggaatgc agcagtacgt cagcaggtcg gtcagttatt
     4621 tggtcacgct attctttaca gtgatgtcaa ggagttgtca aaaacaaact tagatggggt
     4681 ctttattcat gcggccacat cggcccacgc agagttggct agtttatttt taaatcaggg
     4741 gatcccagtc tttatggata aacctatagc tgacaattac ctgatgacaa agaacctcta
     4801 tgacttagcc aaagaaaatc agacttttct gatggcagga tttaacaggc gttttacacc
     4861 tcgtgtcaag aagttgtcaa tttcgtcaac taagcgtaaa gtggctgttg aaaaaaatga
     4921 cttgaaccgg ccaggagata tgacttttaa gctctttgat ttctttatcc atcctttaga
     4981 cacagcctta ttcttgacag aagggacctt actaaaaggg cattttcaat atcatctgga
     5041 agcaggctta ctgagtcaag ttatggtaac cttgatgacc gaaagcatga cgactacagc
     5101 ttctatgaat ttacaatcag ggagtcgccg tgaggtaatg gaagttcaac gggctgaaga
     5161 aacttatcat ttagagaact tagacgagtt atctatttat aaaggtactg aaaaaagggt
     5221 gcttggcttt gcttcttggg acactacctt gcacaaaaga ggttttgaaa caatgatcga
     5281 tgccttttta gaagctatta gcacaggcgt caatcctgtc agccctgagt ctagtctttt
     5341 aagccactgg atttgtcaac agattgctga ctctcagctt tcttatggag agttaacggt
     5401 agagttgcct aaggattagg aattgatatg gaaataagac gaccgacttt aaaagataaa
     5461 gacgctgtat tgtcaatgat taacgaattt ttagagcaga aaagtgctac agatgggtta
     5521 tggcatttta acgtcaatga ctttaactat gaaacatggt tggaagattc cttgcgacag
     5581 gaaatgggtt tgtccagtca aggagtgcct gctattcaat acgtcgcatt tgatgagaga
     5641 agtcaggcta tcgggttctt aaatcttcgt ttacggttaa atgagagatt acttgaaaaa
     5701 ggtgggcata ttggttactc tgtccgtccc agccagcgtg ggaaaggtta tgccaaggag
     5761 atgcttaaac aagctgtcag ctatgcgatt tccaagaata tcacgacaat tttggtaact
     5821 tgtgatgaga ctaatgttgc aagccgagca gttattgtgg ctaacggcgg tatcttggaa
     5881 gatagtcgag gtggaacgga acgttattgg attgaagaac aaagaggcga taagtgatgg
     5941 cagaaaagtg ttggaataat cctaagccaa aggaatggca ggcagaggag ttaagtcaag
     6001 gccggattat tgactataag gcttttaact ttgttgatgg tgaaggggtt cgtaattccc
     6061 tttatgtgtc tggttgttta ttccattgca aggggtgtta taatgctgcg acttggtctt
     6121 ttaaggcggg aatgccttat acgcaagaac ttgaagagca gataatgacg gatttggcac
     6181 agccttatgt tcagggactg acacttttag gtggagaacc ctttttgaat acgggtatct
     6241 taattcctct gattaagcgt attcggtgtg aattgccaga aaaagatatt tggtcttgga
     6301 cgggttatac ttgggaagaa atgatgcttg aaacaccaga caaactggaa atgctgtcct
     6361 taattgatat tctggtagat ggtcgttttg atatcacaaa aaagaacctc atgttgcaat
     6421 ttagaggctc ttctaaccag cgaattatcg atgttcaaaa gtctttggct gctaaagaag
     6481 tgattatctg ggataagtta aacgatgggg accaaacctt tgaacaaatt agtcgggagg
     6541 atttgcttta agaagctgac ttatccacat gatttttttg aaaaggtcat gtgaaaacag
     6601 gtcctttaag ggtgaattgg ggagaaagta attgttattt cctggttttc cccacattat
     6661 cctcatagtt atccacaggt tgtgtataag ttgtagaatt gtgaataaag actaaaaaag
     6721 aggcctaggc ctcttttttg atatagacaa attcatgatc tgttgatagg tctggatggt
     6781 agtaaaaacc agcaaagaca aggcttttaa ggctgtcaac agtttggcaa ttattttcca
     6841 tacaggcagt gagaaatgca cctcgtgcct tttttgaaat ggtggaatgg gttttgaatt
     6901 ggccctcttt ttctgccata aacttgggac ttatccacaa ttgtttacag tctttggaaa
     6961 agacatcgtc aaattcactg gataaaaggg aaatcacttg gggatgctct ttggcaaact
     7021 gattataaca gggccgccag taagacttga ggctttgtcc ttcaattttt attctggtgt
     7081 gaaaatcatg gcggtgctct gcgataggat gattagcggg aatgataccg taaaaggatg
     7141 aggtgatgta aacttgctgg gtgaggtaag cctgttcttg ggtagtgagc ttatcgcgct
     7201 tgatatggcg gtacataagg ccattgaaca gttggtaggc agggtaagcc aagctttgtt
     7261 gagaggccat gtcttgccag cgttgctgtt cttttttagc tgcctcttct ttgatacgat
     7321 aggatttggc caagtcctcc gtggtcatag ctgccatgat ttttaaaatg gcttggctgt
     7381 cttgtggaag gaggtgtggg tgagattcct tagggatcat catttctttt gcggttggaa
     7441 ttaaaaaggt tagcatagtg ttattgtagt aaagttactt gctttctgtc aagaaaacta
     7501 gtcttgtcca aggatgacgt tcacacagat accttcctcg tcttctgtcg tgaaagaaga
     7561 agaatcttct tgtaaaatag ctaggccgtg taatcttgct tttttcagag tagctgaaaa
     7621 aagcaggggc gtctcaatgt gaatggtcaa gaaggctaag cctggggcac cttcttggtg
     7681 ttttttgagg taaggggctg accagtgatt gaatgcaagg tgatgatggt agttccctga
     7741 agcaatccag ctagcacttg gaatggtcat tttatcgcct aaatcaaaaa ccttttgata
     7801 gagaagtgaa gaagccagag catttttgac actcagatgg acatggccaa tgcgtgtgtc
     7861 ttgagctagt aaaaagcgct tggggatatc tgtcaactgc tccaatatgc ttttggtatc
     7921 cgtgggctca gtcacaccaa taatttgacc attgtcgcgg atatcccaat gctcaacagc
     7981 tttgtcatga taaatttcaa tcccattgcc ctcaggatcg ctgaggtaga tagcttcgct
     8041 atagccgtga tcagctgctc cttctaaaga gatacctctg gttaagaaat gattgagaac
     8101 aagtcccaag ctatgacgat caggaactaa aaaggctgtg tgataaagac cataagcctt
     8161 atttgctggt aaaggtgttt gtcgtaactc taagataacg gttttaccgt ctgtagtcaa
     8221 ttgacgagat gtcgtgtctt gtgagagaac ctgtaaaccg ataatacttg tgtaaaaggt
     8281 tgtcatcttt gctagatcgg taacatttag agaaaccgtt cctaaagaga tggttgagtt
     8341 gtaagggtat gtcatcctat tacctccaaa aactaattaa atctatcata atcaatcagt
     8401 ttcaaaaaga aaagtaggca caaataagtg gtataggcac taaaaagtaa ctcatttgtt
     8461 aaccctatct tgtttctgtt ctctctaata gtctataatg ggttataagt tacaaaaaga
     8521 tactaagagg agctgctgcc tatgctgaaa aaaagaccgg cttgtcccgt agaaacggcc
     8581 ttatcggtaa tcgggaataa atagaaatta ttaatattaa gagacttgct aaagggacct
     8641 tgcgttttgg gcagttgaag tcctcaatag gtttggtcag tcaaaaggta cttactgcac
     8701 aattaagggc tatggaagca gacggattgg ttcatcggga agtgtatgca gaagtgccac
     8761 ctcgggtgga gtattcctta acagaaactg ggttgagctt agctcctgtt attgaagcga
     8821 tgtcggattg gggccaaact taccaagaaa aacatgccga tttatttgaa gaaggccaga
     8881 ggaaagagtt gtccgagcat actgagactt agagaagctg agagtttttg aaaaatgata
     8941 ttatcaaatt ctttacagtt tgataaaatt actttttttc tacaaaaaga cttgcgctca
     9001 tctcacgaat atgataatat aatatcaagt ctgtttgaga aaacagaagc tcaggtcaag
     9061 taagtgcaca cttacaataa cacagttgat aatggggaaa ctcagagtca agaaaaagaa
     9121 aagcaagcct tgcattttgc aaggcttttt tctgtcttta ttttggtctg cgccgagcaa
     9181 aatctaaaaa tttaaatttt tccagtttat ggcgactctc ggtgaattgg aattggttat
     9241 tattggaaag gtagactttg gatttgacgg aaacaacatg ttggtctgat ccaaggtcaa
     9301 gtaaaatttt atcacgatcg gttatttggt caatggtaac ctctttgagg gcaaagtcaa
     9361 tggcgagatg gagttccttt tccaaatatt gataaataga atgctctgcg atttcccttg
     9421 tcattattgg caccaaagtt ttactaaggt aatcaatgtc taagacggaa gccacaccct
     9481 caacaacgcg ttggcgtgta acacgccaga ccaggctgtt tttgctaaaa ccggtcaatt
     9541 ttgaaagtgt ctcatccaca atcagtttat caatggcaat gacgttggtc ttagaatcta
     9601 gatttgagta agagacgagt tcctgataac tagttagttc tgagataggg aacatgatct
     9661 gatgatgttt aatcacttgg gtgccacgtc cttgtttttt gaggataaga cctgcctttg
     9721 tgagaagtga gagagccttg cggacggtat ctcggctagc ttggtattgt tgactcaatt
     9781 ctatttcagt aggtaaaaaa tccccttctt tgtaaaagtc tttgtttatt tttgtttcga
     9841 ggtctttata aatacgttca tactttgtca tagggtaaat tacctttcca tttgtctatt
     9901 tttttacaga caacaccttt tattttacca aaataatggc ttttttggca caaacaactt
     9961 tcagacaaat ttgcaatcga ttacaataaa ggtgtcagtc agattctatc taagaatttg
    10021 aatatttcat tttgacgttg cttgttaaaa gcaactagaa caaaggacta ggggatgtca
    10081 catgggaaaa tttgaacagg atgctaagag tcttctaact gctattggtg gtaaagaaaa
    10141 catcaaggct gtcacacact gtgcaacgcg tatgcgtttt gttttgaatg ataataataa
    10201 ggcaaatgtc aaagagattg aaaaaatctc tgtagttaaa gggacattta ccaatgctgg
    10261 gcagtttcag gtaatcattg gtaatgatgt tccagttttt tataatgact ttacagctgt
    10321 ttctggtatt gaaggggtgt ctaaagaagc tgccaaatca gcagctaaaa gtaatcaaaa
    10381 tgccttacaa cgggtgatga ccatgttggc tgagattttc acacctatta ttccggcgat
    10441 tattgttggg gggcttattt taggtttccg taatattttg gagagtgtgc cttttgaatt
    10501 tcttgggcag caggtcgaaa aagggaaatt agtttttgat gcagctgggg atcctgtttg
    10561 gaatacgatt gtgagggtat ctcctttctg gtcaggggtt aaccatttct tgtggttacc
    10621 aggggaagct atttttcact tcttaccagt tgggattact tggtctgtga cgcgtaagat
    10681 gggaaccact caaattttag ggattgtcct tggtatctgt ttggtgtcac cacaattatt
    10741 gaatgcctat gcggtggcag gaacgcctgc tgctgagatt gccaaaaact gggtttggga
    10801 ttttggtttc tttaccatta atcgtattgg gtatcaggca caggttattc cagccctttt
    10861 agctggtctg tcccttgctt atcttgaaat tttctggcgt aaacggattc cagaagtggt
    10921 ttcaatgatt tttgtgccat tcctttcttt gattccagct ttgattttag cgcatacggt
    10981 attggggcca atcggttgga ctattggtaa agggatttcc tttgttgtgt tagctggttt
    11041 gactggtcct gttaaatggc tattcggtgc tatctttggt gccttgtatg ctccgctagt
    11101 tattactggt ttacatcaca tgacaaatgc cattgatacc caattaattg ctgatactgc
    11161 aactcgtaca actggtttgt ggccaatgat tgctctttca aatatcgctc aagggtcagc
    11221 cgtttttgct tattatttaa tgaatcgtca tgaagaacgt gaggctgaaa tatcgcttcc
    11281 tgcagcaatt tctgcttacc ttggggtaac tgagcctgcc ctttttgggg ttaatgttaa
    11341 atatgtttat ccctttgtag ccggaatgat cggatcaggt attgcgggtc tcttatcaac
    11401 aacctttaat gttcaggcaa attctattgg tgttggtggc ttaccaggtt tcatggctat
    11461 caatgtgaag tatatgattc cattcttcat ctgtatggca gtagccattg tggtaccgat
    11521 gtttttaacc ttctttttcc gtaaatcaca tatcatgact aagacagaag atgaagctaa
    11581 actagctgag acacccgttt cggaggctcc tgcggcaact gctccacata agactatgca
    11641 aggaacagtt atcactttaa ccagcccttt aacgggtgaa gttaaagcgt tgtctgaagc
    11701 tgttgatcct gtctttgcac agggagttat gggccaaggt gttcttcttc aaccgacaga
    11761 aggggtgtta gtagcgcctt gtgatgctga agtatcggtc ttgttcccaa ccaaacacgc
    11821 tattggtttg gtgacggctg aaggtttgga attattgatg catattggta tggatacggt
    11881 taacttagat ggtcaaggat ttgaagcttt ggtgaagcaa ggtgatcagg ttaaggctgg
    11941 acaaacattg attcaatttg atatagcagc tatttctgaa gctggatacg ccactgaaac
    12001 gcctcttgtg gtgactaatc aagatgcttt tacggtaact gttgaaggta gtttaccgcg
    12061 tcagattaag gctaatgata agttagcagt agcgatgaaa aagtaggttt actagaagaa
    12121 aacgttcagg aactgagatc agttcctgaa actattttag ggaggaaaac tcatgacaat
    12181 tgataaaaag aaagtcgtct atcaaattta cccaaaatcc tataaggaca ctactggaaa
    12241 tggtgtggga gacttgcgag ggatcattga taaattgcct tacttacaag aactaggaat
    12301 agatatgatt tggttgaacc ctttctaccc tagtccacaa cgagacaatg gctatgatgt
    12361 ttcagattat acggcggtca accctgattt tgggacgatg gctgattttg aagatttggt
    12421 aaaagctgct aaggagcatc agattgagtt aatgctggat atggttttga atcactgttc
    12481 cacagaccac gagtggttcc aaaaagcttt agcaggagac ccttattatc aggatttctt
    12541 tatcttgaga gatcagccga ctgattgggt ttccaaattt ggtgggaatg cttgggcgcc
    12601 ttttggagat acaggcaaat actacttaca cttgtttgat gtgacacagg ctgacttgaa
    12661 ttggcggaac ccacatgttc gtgaggaatt ggctaaagtg gttaattttt ggcgagataa
    12721 aggagtgaag gggttccgtt ttgatgtgat taacctgatt gggaaagatg aagagctggt
    12781 ggattgtccg gtcaatgatg gtaagccagc ttatacggat cgtcctatta ctcacactta
    12841 tcttcatgat ctcaatcaag ccagttttgg tcaggatgat tcgtttatga cagtagggga
    12901 aatgtctgcc acgactattg acaactgtct tttatacacg gctcccgaac gtgaggagct
    12961 atccatggct tttaatttcc accatctaaa agttgattat gagaatggtc agaaatggac
    13021 tattatggct tttgattttg cagcgctgcg agacttattc catgcttggg gtgaaggcat
    13081 gggtcagggc aatgggtgga atgccttgtt ctacaataac catgatcaac cacgtgccct
    13141 gaatcgtttt attgatgtaa cacatttccg aaacgaaggt gcgacgatgt tagcggcttc
    13201 catccatttg tcacgaggaa cgccttacat ttatatgggt gaggagattg gcatgcttga
    13261 tccagacttt gatagtatgg atgattatgt ggatgtggaa agtctcaatg cttactcaag
    13321 cttattagtc tcaggtaaaa gtgcgaaaga agcctttgcc attatcaagg ctaagtcaag
    13381 ggacaatgcc agaacaccaa tgcaatggga tgctagtgaa catgctggct ttacgactgg
    13441 taagccttgg ttagaggttg gcaaatctta tcgagacatc aatgtcgaaa cagaaaaaga
    13501 aggacgtatt tttcctttct accaacgctt gattgctttg cgtaaggaac tccctattat
    13561 tgctgaaggg gactatcggg ctgcttttaa agatagtcag gctgtctacg cctttgaacg
    13621 ccatttaggt gaccagtgtt tgcttgttct caatcatttc tatgctgatg aggtcgaact
    13681 ggaattacct ccacgttatc aacatggaca ggtcttaatc agcaactatg agaaagtttc
    13741 tatttgtgaa aaagtgatac tgaaacctta tcagacactt gctatcttag ctgaaaacta
    13801 actaaaagcc tgaggttagg cctcaggctt ttggtgattg tcacgaaaaa agaagttttc
    13861 tcatatattt atttttaaac tcttattttt tctaaataaa aacaattgag ataagttatt
    13921 ttagctaaat tttggtacaa tggaaaagat tataagtaga gtttaagata aagattctac
    13981 aaatttaaaa tgatataagg agatacgaat gacaacttat caagatgatt tttaccaggc
    14041 tgtgaatgga aagtgggctg aaacagcagt tattccagac gacaagcctc gaacaggtgg
    14101 attttcagac ttagcagatg aaattgaggc attaatgcta gacactacag atgcttggtt
    14161 agcaggggat catgtccctg atgatgcaat tttagcaaac tttgtgaaat tccatcgttt
    14221 ggtggctgac tacgctaaac gtgatgaagt tggagtaagt ccaattcttc ctttaattgc
    14281 agaatatcag tccttaaaat ctttttcaga atttgttgct aatatagcga aatatgaatt
    14341 ggcaggttta ccaaatgaat ttccctttag tgtggcacca gattttatga atgctcagct
    14401 gaatgtgctt tgggcagaag caccaagcat tcttttgcca gatacaacat actacgaaga
    14461 aggcaacgaa aaagctgagg aattgcgtgg tatttggcgt cagtcgcaag aaaagctttt
    14521 acctcaattt ggtttttcaa cagaagaaat caaggatctt ttggataagg taattgagct
    14581 agataagcaa ttggccaagt atgtcttgtc tcgtgaggaa ggctcagaat atgccaaatt
    14641 ataccatcct tatgtgtggg ctgattttaa aaaactagct ccagagcttc ctttggatag
    14701 catttttgaa aagattttag gccaagtacc tgacaaggtt attgttcctg aggaacgttt
    14761 ttggacagaa tttgcagcca cttattactc tgaagccaat tgggatttac tcaaggctaa
    14821 tttgattgtg gacgcggcta atgcttacaa tgcttacttg acggatgata ttcgtgtgga
    14881 atcaggcgct tattcgcgcg ctctttcagg aacacctcaa gctatggaca aacaaaaagc
    14941 agccttttat ttggcgcaag gaccgtttag ccaagcgctt ggcttgtggt atgctggtca
    15001 aaaattctcc ccagaagcta aggctgatgt ggaaagtaag gtagcacgca tgattgaggt
    15061 ttataagtct cgtctagaaa cggctgattg gttagcacca gccacacgtg aaaaagccat
    15121 tactaaatta aatgttatca cgccacatat tggttaccca gaaaaattac cagaaaccta
    15181 tgctaaaaaa gtgattgatg agagcttgtc attggttgaa aatgcacaga acttggctaa
    15241 aatcacgatt gctcacactt ggagtaaatg gaacaagcca gttgaccgta gtgagtggca
    15301 tatgccagcc cacttagtca atgcttatta tgatcctcag caaaatcaga ttgtctttcc
    15361 tgcggcaatc ttacaagagc cattttattc tttagatcag agttcttcag ctaattatgg
    15421 aggaattggt gcagtcattg cgcatgagat ttcacatgcc tttgacacca atggtgcttc
    15481 ctttgatgaa catggtagcc taaatgattg gtggacacaa gaagactatg ctgcctttaa
    15541 agagcgtaca gataagattg tggcgcagtt tgacggctta gagtcacatg gtgctaaggt
    15601 caatggtaaa ttgacagttt cagaaaacgt cgctgatcta ggtggggtag cttgtgcttt
    15661 agaagcggca caatcagaag aagacttctc agcccgtgac ttctttatta actttgcgac
    15721 gatttggcgt atgaaggccc gtgaggaata catgcaaatg ctagctagca ttgatgttca
    15781 tgccccgggt gaactgcgaa caaatgtcac cttaaccaac tttgatgctt tccatgagac
    15841 ctttgatatt aaagaaggcg atgctatgtg gagagctccg aaagaccgtg tgattatttg
    15901 gtaattaggt agttgataaa ccttcaaatg ctttgtgatt ataaaaatga ctgattctgt
    15961 gaaacacagg atcagttttt tgcttcctta ttggtgtccg ttttgataca acaaaaagac
    16021 aaccctcagg aaagaaggtt gcctttttta gacttatcag aaataagtga ttagttgtta
    16081 agggcagcag ccatagtagc tgcaacttct gattcaaagt cgttagcttt tttctcgata
    16141 ccttcaccaa cttcaaaacg tgcaaaagca atcgctttag cgtttactga gtcaaggtaa
    16201 gcttcaacag ttttgctgtc gtccatgatg taaacttgtg caagaagagt gtaagcttgg
    16261 tcaactttag tgttgtcaag catgaagcgg tccattttac ctgggatgat tttgtcccag
    16321 attttttctg gtttgccttc agcagcaagt tcagctttga tgtctgcttc agcagcagta
    16381 atcacgtcgt ctgataattg agcttttgaa ccgtatttca agaatggaag tgctggtttg
    16441 tcaaccattg cacgcgattc gttgtcaagt tcgatagcgt ggttcaattg agcaagttca
    16501 tctttgatga attgtgcatc aagctcagtg tatgaaagaa cagttggttt cattgcagcg
    16561 atatgcattg atacttgttt agcaagagca tcgtcgccgc cttcgacaac tgagataacc
    16621 ccgatacggc caccattatg ttggtaagca ccgaagtgtt gctcatctgt tttttcaatc
    16681 aaagcaaaac gacggaatga gattttttct ccgatggtag cagttgcatt aacgtaagct
    16741 tcagcaagag tttcacctga aggcataaca agtgcaagtg cttcatcgtt gttagctggt
    16801 ttgccttcag cgataacttt agcagttgcg tttaccaatt caacgaattg agcattttta
    16861 gcaacaaagt ctgtttcagc gttaacttca acaacagcag caacgttacc atgaacgtaa
    16921 acgcctgtta aaccttcagc ggcaacacgg tcagcttttt tagctgcttt agccattcct
    16981 ttttcacgaa gaagttcaac ggctttgtcc atgtcaccat ctgtttcaac aagtgctttt
    17041 ttagcgtcca taacgccagc accagatttt tcacgtaatt cttttacaag cttagctgta
    17101 atttctgcca tgtgtagtat cctccaaatg atatttgttt tggtttcaaa aaacgagaca
    17161 gagcggtttg ctttctgccc cgtttggttt tcgattgtta aagacaaaca aagtttgcca
    17221 taagagttag gaactctgtg ctaattaagc gttgtcgcct tcgacaactt caacgatttc
    17281 ttcgattgaa tctgcttgag tgtcagcttc aaaagcaatg tctgcatctt caccttgacg
    17341 gccttcgata atagcgtcag ctaatttagc agtgattaat ttaacggcgc ggatagcgtc
    17401 atcgttagct ggaatgatga tatcgatatc atctggatca gcatttgtat caaccatcgc
    17461 tacgactggg ataccaagtt ttttagcttc tttaacagca atttgttctt tatgtgggtc
    17521 aacaacatac atcacgtctg ggatacgagg catatcttcg ataccgccca agaatttttc
    17581 aagacgagcg cgttgtttgt taagaagtgc aacttctttc ttaggaagaa cgtcaaaggt
    17641 tccttcttct tccatacgtt tgatttcttt caaacgagcg atacgttttt ggatagttcc
    17701 ccagttggta agcgttccac ccaaccaacg gtggttgatg aagtattggc ctgcacgcgt
    17761 tgcttcgtca gcaactgctt cagcagcttg ttttttagta ccaacgaaca agatgacagc
    17821 atcgtttgct gcagcatcac gaacaaattc gtaagcttgg tcagctaatt ttacagtttg
    17881 ttgaagatcg ataacgtgga taccgttacg ctctgtgaag atgtatttag ccatcttagg
    17941 gttccagcga cgagtttggt gaccaaagtg aacaccagcc tcaagaagtt gtttcattga
    18001 aattactgcc atgagtagtt tctcctttat aaaatgtttt ttcctcttcc agacttcgac
    18061 ttgcaggcta acctagcggc aacaagccca caattggtct agaatgagta tttgccgcct
    18121 acacgaccct actatattat caaaaaaaga ggttaaaagc aaggagaaat gctatggatt
    18181 cccttatttt tgagatgact agcttctttg tttattggta agtgttaaga caagcttttg
    18241 ctcattttgg gtgacacttg ccaaatcgtt ttatttaaga tatagtgaac ttgactaatt
    18301 tttggaggcg ttttcatgaa gaagggtatt cgatttcaat tgcttggcag tccccatatt
    18361 tttcttgatg acaaagaaca attttttgcc tttgccaaag caaatgctct cctttattac
    18421 ttagtggtta atggatctgt tagccgcgag gtggcggcga gtctactttg ggaaaataag
    18481 aacacgcaaa cagcgaaaaa gaatttacga aacgctatct accaggtcaa taaagtcttg
    18541 caagctgatg tgattatttg tccaaatcgc aacctgctgg ttttgaataa aacacttgat
    18601 atcaaaacag atattaacct ttttctggct gatccgctag ctcacttaga actctaccaa
    18661 ggggagtttt tgcagggctt ttaccttaaa agtggtgagg aatttgatct ttgggtttca
    18721 aaaatgcgta tgcagtatga acaggtatac ctaaaagcgt gttaccaaaa aattgaagaa
    18781 aagctctctt tagatgccat tgaagatgtc gaggaacatt taaagcagct tattgaacgt
    18841 gatgaatttg aagagaaaaa ttatcagctc ttgatgaggc tttatcagca gggaaattgt
    18901 cctggtaagg tcatcgaaac ttattatcag ttggctaatg tgttggataa ggagctaggg
    18961 attcagccaa gtttgcaaag ccaacagatt tatcaggaag tggtcgctaa agatcgtaat
    19021 gaacgcaaaa tcaagcattt tttgcgcaac agtaatcatt ttttggggcg gattgatgag
    19081 attaagcagc tggaaaacta ttttgccaac tgtttggcct gccaagaggt aggagctctg
    19141 ctcttaattg gtgatacagg gattgggaaa cgaaccctag ccagacaggt cttagccaat
    19201 caaacccaaa cgtttcaaat tgtcacagct aaatgttttc gtgaagaggc tatggattcc
    19261 ttattgcctt ggcgtaatat cttagatggc ctgggagatt tggtgattca aaaccgctta
    19321 ttgaccacca aagcttggaa ggctgctctt aaacgctgtt ttcctgtggc aaccattttt
    19381 caagaagata acaaccaacc ctttatcaag gaccacacca gcttgttggt ttcctttatt
    19441 gttgatattt tacaacattt ggcagaaatc aaggcgctgg taatccttat tgaggattgt
    19501 cactggatgg atgaggacag tttgaccttg ttgcaacggg tcatgaatca attggtccac
    19561 tatccgattg cttttgtctt gaccaagcat ctaggaacga ctcctgagct cggtctttgt
    19621 ttgaatgctt tgatgagtca ggggcgttta gaaagcattt gtttggagcc ttttaatcgg
    19681 caagaaagtt tggcttatat taacagtcaa ctgggtagtc agccagtgac ggcagaagaa
    19741 atggagcacc tctatcaagc cagccaaggc aaccctttct ttttgtcaga atacactcag
    19801 gccctactgc gtcacgaaaa atttgtgcct ttaacgtctg ccattaaggc taagttgggt
    19861 cttaaactag ctaatctaag tagtcgtgat gacgcattgt taaactattt gtcttgttgt
    19921 cggaggccta tccctctaaa taccctcgct caattgatgt tactgccttt agaagaagtg
    19981 atcgagatgg tggataatct ggggcactac tatattttgg tagaagaaag cgtaggagag
    20041 gaagtgttga tttcatttcg tcagcggatt attcaactct atagttatga ccgtttatct
    20101 ttgtcaaaaa ggcgtttatt gcacggacaa attgccaaac ggttagaaga tttactgcct
    20161 attttgacac caagccctca cttattagat gacattgctt accattatca ggaatcacgg
    20221 caggtgatta aggctttgga atacaacctt aactatctag atgctacctt gccatttcag
    20281 catgagctgt ttcccattta ttcaaagagt attggctcct tggaaaagtc agatcgtgac
    20341 catcaacgct taatggaaga gcagtttgat aagattcggc aaagcattgc ggatttggaa
    20401 ctgacctatg ataataaccg tgatttccag cagttgctta ttcgtttttc ttaccttgaa
    20461 ggccgttatg atattcgaac agggaggtat caagaaggga ttaaacacat tcaaaaggtc
    20521 atcgctttgg caaccgagct aaaacaaccc tccttcttgc tagaaggcta tcgccagtta
    20581 attcattact gtatccaagt ggaaaacaag ccagaaatgc gttattacac tagtctttca
    20641 ttggaggcag cggttgctgc taatcatttt gaggccattg cgattagttt gcggttaaac
    20701 ggtctctatc atttgattat tggggagtta aatgaggcag agcgtctgct tcaacagtcg
    20761 attgacttct ttaaggtgac gccaggcttg caagctaact atgcgattca gattgctgct
    20821 gccttagatt atttggggga gattgcccag attcgctatc agtttgaaaa ggccgttact
    20881 taccaaaaac aagccattgc cttgaccgaa aacaagccag ctgagttatc ggcgagtatt
    20941 ttttatattg gattagggat ttcctacttt tatttagctg attttgaaca agctgagcaa
    21001 atcttgagtt tggctaagga agctttggtc aatcacagtt atccttggaa agaaacgcaa
    21061 cttgaaattt acctggccat gattcaatgg aaaaagggca attatcagcc agccttgacc
    21121 ctacttgatt acagagaaac cttgatgtct aggtatcgca atccgcgtga taagggcttg
    21181 gttttctatt tgatggctgt ggtcaagtac caattgatat ttcaaggagc taccctaagt
    21241 cagcaggaaa aagaaatggc agaccacctg ttgtcagaat cctttgagta ttattatgag
    21301 attgctagta ctaacctcaa cccttaccga gattgtcatt tggtgagtga gttgaacgat
    21361 ttgcgtcaac aattatctgc taaaagctaa ggaaaaccct ctgaagtgaa tcagagggtt
    21421 ttagctatgt tgtgccataa tctgtacgag gtaaaagata aaggtggctg ctaaattggc
    21481 ggtgtggtta tcaatatcgt gtggtggtga aacctcaacc acatcaaagc caactaattt
    21541 tccgctagct gcaatgtgtt gcaaaactaa gacggcaaga tttgggtcaa caccgaggga
    21601 ctggatagca ctaaccccag gcgcagcacc aactgagaaa caatccatgt caatggtcag
    21661 gtagaccctt tcttgccctt ctaagaaacg gtcaatggct cggcagacct tttgatggcc
    21721 catttggtag atgtcttgac ctgttaaaaa ttgaatgccc ttggattttg ccacaaagtc
    21781 aaataggaaa aggttgttat tatgttcttg aatgcctaaa acgaagtatt taaagaggcg
    21841 tttgtcagca accgcatcgt caaacatctg tcgaaagcct gtccctgaat tagggcctgt
    21901 ttggtcgtaa ggccttaaat caaagtgggc gtccatattg atgacagcta ggtcatcact
    21961 tggggatagg ctttggcgta ggcctaagta gtggccgtag gcggtttcat ggccacctcc
    22021 taagacgatg ggtttgagat tgaggtcaca catccgtttg atggctttgc ttaaactgtt
    22081 ttgcagttgt tccaaagagc gattggggcc gtcaatgtta cccacatcgt aaaccataac
    22141 ttggtttcct aaatgccaag ggaatttggc gagctgggtt cgtatggcag ctggactttc
    22201 gaccgctccg acacgaccat tattgatata aaccccctta tcacttttaa agccaatcaa
    22261 ggcaaaatgt gtcccttcaa atggggttag tgagctgtcg ttaaggtcaa ggaaagtcat
    22321 gaccatgccc catttggcag tatataagtc atcgtcgatc ccaccatgat aatagctagt
    22381 ggtggatggg tagtaatctt ctagcatgcg cgtcatctcc tttaggatta tgatagcaaa
    22441 ataaaactta cagtcaacac tttcttggcc tttgtcactg gtttttagga atagcagagc
    22501 aataaaatcg tctgcttttg gtcccggtta gttagtctaa agaggccatg acctctgcca
    22561 cagcccctct agttaaagaa gtaccttatt cactaaaatt ggatactcaa ctcaactgcc
    22621 tgctcaaccg cttcgaggaa gcttgggtct tcaatgactg ctgatgcctt gtttagttca
    22681 tcgtagattt cgatgtgttt atcgtgttcg ataaagttga cttccttgcg gaagagatcg
    22741 taggcaacct tagtcccttt acctagttca tgattttcag gtttgagatc aagggcttgg
    22801 caggctgcca tgatttcagt agccacgatg cggcgagaat ttttaaggat ttcaaaggcc
    22861 ttgcgagcag cggtggtgcc catgctgaca aagtcttctt ggttctcaca agatgggata
    22921 gaatccacac tggctggatg tgccaaaacc ttgttttcag aagcaagaga ggcacaggcg
    22981 tactgggtaa tcataaagcc tgaattgaga cctgggtatt tgacaaggaa ggatggcaat
    23041 ttgctgagct ggctattgac caaacgttcc acgcggcgtt cagaaacatt tccaatttca
    23101 gaaatggcga tgcctaagaa gtcaaatggc tgtgccattg gttctccgtg gaagttacct
    23161 cctgaaataa cgtggccatc tttacagata attggattat ctgtgacaga attgatttca
    23221 atgtcaactt tggatttgac ataggcgata ctgtctttgc tagccccatg aatttgaggc
    23281 atgcagcgca aggtgtaagg gtcttgcaca cgagattgag tagctacggt tgtgttttgg
    23341 ctgccttcaa ggaggtttcg gatattgcgg gctgttgcca actggccgct ttgtgggcga
    23401 atggtgtgaa ggttttcttc aaatggacta gtaatcccgt tatgcacttc aagggatagg
    23461 gcaccagcta ggtctgacag tttcaataat tgaatggcgt cataggttgc aagggcaccg
    23521 actgcagtga gaacagttgt tccgttgata agggcgagtc cttctttagc ggctaaagaa
    23581 atcttgtcaa taccagcttt atcaagagct tcttgaccag aaagaagctc tcctttgtag
    23641 taagctttcc ctagaccaag cataggaagc accatgtgag caagcggcgc caaatctcct
    23701 gaagccccta gagacccttt ttcaggaatg taaggatgaa cccctttatt gagcagttcc
    23761 aacagttttt caatggtgga tagacgaata ccagaatacc ctttgactag tgaattgata
    23821 cggatcagca tgatagctct cacggcatct tctggcaatg ggtcaccaaa tccactagca
    23881 tgggtacgaa tcaaattttc ttggagttga actgtatctt ctggtgagat gctcacgtta
    23941 caaagggatc caaaaccggt ggtcaccccg taaaccaccc gtttttcact gacaatgtca
    24001 tcaacaattt tacgagaggc attcacagct tcaatagcac tatcatcaat gtgacaggct
    24061 acgccttggc gagcaatcgc aatcacgtct tcgatagtaa ggctttcgcc atctaaatta
    24121 atcactcttg tcatagtttt tctcctagat ttgttcctta gggggcattt ggtccatttg
    24181 gtggttacgt ccgtgcataa ggtagtacac actacttgca atgatgacac caactgcacc
    24241 ataaacaaag ttcattgggt tgtcacccca aatcataact aaactaacga taactgcaag
    24301 aattgggata atagggccaa atggtactcg gaagatcaca ttagcatcgg gatcgtcctt
    24361 ccgtaatttc ataacggcta gtgctgttgg aatgtattgg aagaagcgga aaacaacact
    24421 gagttttgct aagctttcaa atgagccagt aagcaggagt acaatggcaa ttgctcctga
    24481 aaccaagata gcaacgagtg gcgcgccatt ttgattttgc ttggcaatag cagctggtaa
    24541 gagcccttca tcagcaatgg cagcaccgta acgtggcacc atgatggatt ctcccatgtt
    24601 aagacctgta atggaaatta aagcaccgat tgagaccatc caagcgccag cagggcctat
    24661 cattttaaca aaggcatctt gaacaggtgc attggtcatc atgatttgag agccaagcat
    24721 ggcaatggtt ccaccgataa tcagcatata taagacagag acgatactga ttgatcctaa
    24781 aagagcacgc ggtacatttt tttcagggtc acgcatttcc ccagcgacaa tagagagggt
    24841 ttcaaaaccg ataaagccat agaagatata aacagcagta ttggagatag cccctaagag
    24901 gttagtccct ggttccaact gaacaaatgg ggtaaagtta ggaagcccgt ttttgatgaa
    24961 gaaaagggta caggcacaaa aggcaacgat tggaattaac ttagcaatgg tggctgtgat
    25021 ggtgacaatt ttagaggttt tcaaaccagc gatattcatc aaagatagta agatgattaa
    25081 cccaatacta agtggaatgt gccaaccttc aaaggcaggg aaagtaatga taaacattct
    25141 ggcaaaccca gctgccatgg ctgcccaagc gaagatggta acggtccaac caaggaagcc
    25201 aacgttaaag ccaataaaat caccaaaagc tcgtttggaa tattggaagg ctccgccgtt
    25261 tttcccaaaa taaccagaga cttctgcaaa acaaactgcc aacatgatgg tcaaaatagc
    25321 tgttccaaac ataacagcga tggatgcagg gccaaggccc ttataaatgg ccctaggtaa
    25381 gagaaaaata ccagagccga taacggcatt gatcccgtaa agagtggcac cgctcaagct
    25441 aaactttgcc tgttctcttt cttgttcgtt catgtgattt gacgccataa taatgctctc
    25501 ctttttgttt gcaaattgcg ttcaaatata atggataagg tcgctaggaa atgaggagat
    25561 agtcggttta ggaggatcct tcttgcacct aacgatagtg atgagctatt ggttagcgat
    25621 gagaaatagg tagccttttg taatgatgtg tagtgatttc attggagaag aataggcatg
    25681 agtagtgtga tgcctatctt tatcccacta agtcttatac cataagcctg cccaaacagc
    25741 tactggagtt tgggcggggc ttgtttgatt gaggcaaact tttagatctg aaaggctggc
    25801 ctcctgctcg gttaccaaca agtgattggc ttcaagctct tgattcgcct gattggtcac
    25861 agaataagtc agtttgctta gggcataaag cagttgacag tccgctttag gtgcctcaaa
    25921 tggtgtgtca gaccagacgg gtaataattg cccttggtat tggtcatcat acattaaatt
    25981 gatgtcttgg caggttccac gactgacgat tctggtatcg ccttcaaacg catagggttg
    26041 aaaaggagct agggaaatct ctttagcatg actcaaatca cttaatgtga tgggcttgtc
    26101 taggctcatt agaatcctgt gatagccatc aagtcgggta aaggttgtct gtcgtagggc
    26161 tacagttgca gttgagagac gataagagaa ttctctcttt ttgtaatctc catcttcggg
    26221 ataaaggaac aattggttgg tttcaccgcc agaccagtca gtttggaggt aatcagaggg
    26281 cgctttgatg gttacctttg tcatttgttc tctccttatt tccaattaga atagtcctgt
    26341 gatgttgccc tcctcgtcaa tatcaattgt ttcgctggca ggtacttttg gaaggccagg
    26401 catggtcatg atagcacctg ttagggcaac gataaatcct gctcctgcgg acactttaag
    26461 gttgctaata gtcactgtaa agtcagttgg tgcgccaagt tttttggcat cgtctgagaa
    26521 ggaatattgg gttttggcca tgcaaattgg gtagttgcca aagccgaggc gttctaagtc
    26581 agccagttca cgtttggctg ctgagctcaa gttgatgcct ttaccaccgt aaactttggt
    26641 aacgatttta gtcagtttgg tttcaatgct atcgtcttcc tcataaacaa agcggaattg
    26701 attgtcttgc tcggctagtg tcacgacttt ttcagccagt tcacggccac cagctccgcc
    26761 atttgcccaa acatcagaaa tcacaacgtc aacgccacgt ttgtcgcagg catcatagac
    26821 agcttgtaat tcggcatcgg tatcaagcgg gaatttattg atcgccacga caactggaag
    26881 gccatagacg tcttggatat tagccaaatg tttgtcaagg ttaggtaaac catccacaac
    26941 ggcctgaaca ttttcagtag ccaggtctgc ttttggcaca cccccatgca ttttaagggc
    27001 acgtatagtg gctactagga caacggctgc tgggcgaagg cctgacatgc ggcatttgat
    27061 gtcaatgaat ttttcagctc caaggtcagc gccaaatcca gcttcggtaa cggcataatc
    27121 accatatttc aaggctagtt tggtggcgag gacactatta cagccatggg cgatattagc
    27181 aaatgggcca ccgtgaatga gggctggtgt gtgttccagg gtttgcacga gattaggatg
    27241 aatcgcatcc ttgagcagag cggctagggc accaccagct ttcaagtctt tagctgttac
    27301 aggttcacct tggtagttgt aaccgatgat gattttttca aggcgggctt tgaggtcaga
    27361 aatattttcg gataggcaca ggattgccat gatttcagag gcgacggtaa tgtcataacc
    27421 atcttcacga ggaatcccgt tgaccttgcc ttgcaagccg tcaacaatat gacgaagctg
    27481 gcggtcattc atatccacca cacgtttcca agtgatacgt ctggaatcaa tgcctaggct
    27541 attgccatgg tgaatgtggt tatcaatcaa ggcagctaat aagttgttgg cgacaccgat
    27601 ggcatgaaag tcccctgtaa agtgaaggtt gatgtcttcc attgggacaa cttgcgcgtg
    27661 gccaccgcca gcagctcctc ccttaacccc aaaaacagga ccaagagaag gttcacgaag
    27721 ggcaataact gctttttttc caatggcaga aagagcatct accaatccaa cagaagtggt
    27781 ggttttgcct tcaccagctg gtgttggtga gatagcagtg acaagaatga gtttgccatc
    27841 aggtttgttt tttaaggcga ccagttgacg ggcatcaatt tttgccttgt acttaccata
    27901 gagacagagc gcctccttgt caatccctaa ttgatcggca accttactga taggttccat
    27961 agtgacagag ttggctattt caatatctga taaaaccata gttgctcctt tacttgttaa
    28021 acaatatcga gaattttcgt gtagatgtca tctgccaaat gacagccctt gtctagtaag
    28081 gcttgtcctt tttggcgata gtcggtaaca aagtcttcat ctttgatgcc agacagatta
    28141 atcaaaacat tcaaccaagc gccttgtaaa cctgctttta agtttaaagc agcaactcct
    28201 aagtcgctag cagcatttgt attagactta ccgacagctg ttgcagtgat ctcaagtgct
    28261 tcgaccatga gggtcatcat ctcaaaaggt gattgtgcag ctgttttcaa ggccttttgc
    28321 atggcagttc gtctagcagc cttgtcttcg tcagtctctt ttggcatatc aaaaacggca
    28381 gaaaccaggt tgaaggcttc ggtatctttg tcaatggctg caagcaagct agcttgtaga
    28441 gcggtgcttt tagcatggat ctctgtaata atatcttggt agtcagcgta ttttttctta
    28501 ccaagagtca attcacagac catcttggtt aaggaaatgc cattagcgcc agagagagca
    28561 gcagcagaac caccacctgg agcaggggca tctgagccaa ggactttagc gaaatctgtt
    28621 aagcttaaat caactaaacc catagagaac ctcctaacct agcaaatggt tttcaagaat
    28681 ctgtttatgg tagtcaaagt cctcaacttg taagtagtat tcagccacat cgattaaggc
    28741 cttggctggc gctaagccga tgacttcaga accaataaca ttcacaccgt agcgacgcgc
    28801 ttcaaatttg atggtttcaa aagtacggta aagagaacat ttttcaaagt taaccatgtt
    28861 catggaaact tgggcaatat gacggtcttc taacatgaca ccgatagcct tacaatattt
    28921 gtagccacca cctgaaccgc ggataatttt cgcaatttta tgagcaatgt cgatattgtc
    28981 agtatccaaa ttaacattga aggcaaccaa tggcatgcga gcaccaacgg cagttacccc
    29041 agcagttggg tgaatcttac gatctccgta gtcaggtgcc caatcctctt ccaacagttt
    29101 ttctggcatg ccttcgaatt gtcctttacg gactttggcc aggttttgac gttctgggcg
    29161 tgtggcagaa tcttcataaa ggaagatcgg gattccaagt tcacggttaa tccgttctgc
    29221 gacttgtttg gaaatctcaa cacattcttg tgtagtgatg tctttgatcg gaacgaatgg
    29281 gcagacatcg gttgctccca tacgagggtg ttcgccatga tgtttggtca tatcaatgtt
    29341 ttcagaagcg tatttcacca gttggaaagc tgcttcttga atagactggt catctccaac
    29401 caaggtaaac acgctccggt tgtggctcgc atcagaagag tagtcgagaa gggtcactcc
    29461 aggaatactt ttggccgttg ctaccaaacc atcgataaca gcctgatttt ggccttctga
    29521 gaagttggga atacattcaa caattttcgc catgttaatc tccttttatg tcattgagtt
    29581 actcattaag ggaataggtt acctacttta gataacgctt acatttttag ctagcagtta
    29641 ttagtgaaat aattgatcta ccgctgaagt cacaaggtca tcatcagcca agtatgggat
    29701 agtgatgtga tcagtacctg catggagacg gttgtattca atagcggttt caatagcgtg
    29761 ctcattgcga gcccaattgc gacgagcaac accacccatg gtgtcccaag caatggcaga
    29821 tttaataatc tcatcaatgc gttcgctgcc atctaaaaca agaccgaaac caccattgat
    29881 ggctttacca ataccagttc cgccaccgtt atgaagagcc actaaactca taccacgcgc
    29941 agcgttacca gcgtaacatt gaaccgccat atcacaggtc acattggaac cgtctttaat
    30001 gtttgaggtt tcacgaaatg gtgagtcggt accagacacg tcatggtggt cacgaccaat
    30061 catgacagga ccaatcttgc ctttgcggac caattcatta aatttaaggg cgatagtcac
    30121 acgaccgata cagtcttggt aaaggatccg ggcttgggta ccaactacga gttgattttt
    30181 ctcagcatca cgaatccagt tgtagttatc acggtcttga taacggcggt caggatcgat
    30241 agcttccatg gcagctttgt cagtagccac caagtcttcg tgtttaccac tcaagcagac
    30301 ccaacggaaa ggtccatagc catagtcaaa aagcataggt cccatgatat cttccacata
    30361 ggatggccag ataaagccgt ctttgtcgtt acgaccattc ttagaaattt ctgtaatacc
    30421 agagtcgtaa acagacttca taaaggcatt gccgtagtca aagaaatagg taccattttc
    30481 agttaacgtc ttaatagctt caaaatgccg tgccaaggta tcatcaacca tttggtggaa
    30541 ggtatcttta tcttctgcca agagccgggt tctttcgtca aaagtgatgc cgactgggca
    30601 gtaaccacca tcgtaaacat tgtgacaaga ggtttggtca gacaaaagat caacatgaat
    30661 ctgcttgtca ttgacgtact ctaaaagatc aacgatatta ccatggtaag caatcgaagt
    30721 ggactcttta gcatctatgg ctttttgggc taattgcaga gcttcttcag ggctttcggc
    30781 aatttgactg atccagcctt gagaatgacg ggttttaatc cgagattggt caacttcagc
    30841 aataatggca actgctttag caatttcagc agccttacct tgtgccccac tcatgcctcc
    30901 tagaccagat gagatgaaaa gcttaccggt caaatcaccg tcatcagcca ctccaagttt
    30961 gagacgacca gcatttaaga gggtattaaa cgttccatga acgattcctt gtgggccaat
    31021 atacatccaa ccacctgcgg tcatttgacc atagttggtg acacccatct cttcagcgat
    31081 ttcccagtct ttcatgttgt cgtattcacc caccaaaagg ccattagtaa taatcacacg
    31141 aggcgcttca ggtttggatt tgaaaagacc aactggatga ccagactcaa ccaccaaggt
    31201 ttgttcatca gtcatgactt ctaggtattt tttaatcaaa caatattgca tccagttggc
    31261 acaaacagaa ccagtttctc catatgtgac tagttcgtaa gggtacaagg caatttcaaa
    31321 actcaagtta ttgtcaatca tgacctgcat ggccttggca gctgtacaat ttcccttgta
    31381 ctcgtcaatg ggtttgccgt aaatgcggtc ttttggtcta aagcggtagc cataaatacg
    31441 gccacgggtt ttgagttctt ccaaaaattc tggaatgact tcttcgtgga acttcgtagg
    31501 tacatagcgt agggcatttt tcaaggcgat ttcggtctga gcctgggtca atcgaaaacc
    31561 tctgtctggt gctcttcgga taccttcttc aaacacagtc ttttccggta aaacatcgtc
    31621 taacttaaca gtcatagcgg cagctatgtc agtttcactg taaaatgaca tagaaaactc
    31681 ctcctcaact cctttttatt gtcgagatta gtctaactta gtctagtcta taaaaagtca
    31741 accacgcttt gaaatgcgaa aaaagtctaa aaaaaaatca aaacctcatt ttttgactaa
    31801 aatttgacgc ttaatcagta cacttgaggt atgacagata aagtcagtca gaggttaatt
    31861 gttgttacca aagttgcaaa gatcgcaact attgagtggg tgagggggga ctagtaatgg
    31921 ctattaatca gttgacaaac gatgttaaaa aaggaggttt ttatggttgc tgatgtctta
    31981 ttaacacatt ttaatcaact attttgtcta aatgatccag gtcatccttt aactggtcaa
    32041 gaaatgaaaa aagccactat cgttgaagat ggctatatag ccatcaaaga cggcctcatt
    32101 gtggctcttg gttctggtga accagatgct gagcttgtcg gtacacagac tattatgcgt
    32161 tcttataaag gtaaaattgc aacgcctggt attattgact gtcataccca tttggtctat
    32221 ggtggcagtc gtgaacatga atttgccaag aaattagcag gggtgtctta cttggatatt
    32281 ttagctcaag gtggtggtat tttaagcacg gtgagggcca ctcggtcagc cagctttgac
    32341 aacctttatc aaaaatccaa gagattgcta gactacatgt tgcttcatgg agtgactact
    32401 gtcgaagcga aaagtggcta cggccttgat tgggaaacag aaaaacgcca actggatgtt
    32461 gttgctgctt tagaaaaaga ccatcccatt gatttggtgt ctacgtttat ggcagcccat
    32521 gcgattcctg aagaatacaa agggaatcct aaggcttatc tcgatgtgat tatcaaggat
    32581 atgcttcctg ttgttaaaga ggaaaatctg gcagaatttt gtgatatttt ctgtgaaaaa
    32641 aatgttttta cagctgatga gtctcgctat ctgttaagca aagccaaaga aatgggcttc
    32701 aagcttcgta ttcacgcaga tgaaattgcg tctataggcg gagttgatgt cgcagcagag
    32761 ctcagcgcag ttagtgcaga gcatttgatg atgataacag atgatggtat cgctaagttg
    32821 attggtgctg gtgttattgg taatttgctt ccagccacca cctttagttt gatggaagac
    32881 acttatgccc ctgctcgcaa aatgatcgat gctggcatgg ccatcaccct ctcaacagat
    32941 agtaatccag gcagttgccc gactgctaac atgcagtttg tgatgcaatt aggttgtttt
    33001 atgttacgtt taacaccgat tgaagtctta aatgctgtta ccattaacgc ggcctactct
    33061 gtcaatcgtc aggagagagt tggtagtttg acagttggta aagaagcaga tattgccatt
    33121 tttgatgccc caaatattga ttacccattt tatttctttg ccaccaactt gattcaccaa
    33181 gtctataaaa aaggacaact cacagttgac cgaggtcgta ttctttaaaa aatttaaaaa
    33241 agaagctttg agcgttagct caaaacttct tttaaatatg ctgttttatt gacgaatcaa
    33301 gtagtcaaag gcaccgatgg cagcagtagc accagacccc atggaaataa tgatttgttt
    33361 ataagctgaa tcagtacagt ccccagcagc aaagatacca ggaatattgg ttgacccatg
    33421 tttatccaca atgatttctc cgcggtcggt taggttgaca ccactatctt tgagccaagc
    33481 tgtgtttgga acaagaccaa tttgaacaaa gaccccttca aggtcaaggt gtttgtcttc
    33541 accgctatca cgctcagtgt agttaagacc tgtaacatgg tcctctccaa caatgtcctt
    33601 agtagcaaca tttttgatga tggtcatatt gttagtctta gctgcgcggt cttgaagcac
    33661 tttgtcagct ttaagttcag gcaagaattc taaaacgtaa acgtgtttag caagaccagc
    33721 caagtcaagg gccgcttcta atccagagtt cccaccaccg ataacagcaa catctttgcc
    33781 ttcaaagaga ggaccgtcac agtgagggca gtaagtcacc cctttattac ggaattcatc
    33841 ttcaccagga acattaatgt tacgccattt ggccccaaga gctaagatag cagttttggc
    33901 ttgtagaacc gcaccattag ctagagtaac ctcgatgttt tcttttttct caatagaagt
    33961 agctagttgt gctttgataa tatcaacatc ataagactta gtatgggcct ccacttcggc
    34021 cattaatttt ggtccttcag tgtaaagggt accaatcatg ttttcgatgc cgaccgtttc
    34081 catgacctga ccaccaaagg tttcagcaag taaacctgtt tttagtcctt tacgagctgc
    34141 atagatagca gcgctattgc ccgcagggcc accaccaata acaaggacat catagaggcc
    34201 tttatcagca aaggcttcct cagaaagtgg gccagcgatt tgttctaaga gttgctcaat
    34261 ggtagcgcga ccagaagtga attcttcccc gtctaaaaag acagtaggga cagacataat
    34321 gccttttgct ttcacttcat cttggaacat gccgccttct accatagtgt gtgaaatctt
    34381 gtcgttcaag acggacataa tgtttaaagc ttgaacaaca tctgggcagt tatggcaggt
    34441 taagctgaca taagtttcaa aatgcaacgg acggtcaatg gccttgatgc gatcaataac
    34501 atcttgatcc acttttggag cacgtccaga tacttgcaag agagcaagaa taaaggacgt
    34561 caattcgtgg cctaaaggaa gaccagcaaa gacaacccca ctaccgtgac cttttttcgc
    34621 aaccttaaag cttggttgtc ggtctaaagt gatgttttca atagaaatac gctcagacat
    34681 agcagctatt tcttccacaa agtctttgac tttttgagac tgctcattgt caccaaggga
    34741 aacttgcaaa acaagatcag cttctaacag ggtgaggtat tgggcaagtt gttctttaat
    34801 atcaggactt aatgccatag ctttcctcct aaaatttatt aaattttacc aactaaatca
    34861 agacttggtg tcaaagtttc agcgccttct ttccatttag ctggacaaac ttcacctgga
    34921 tgtttacgga cgtattgggc agcgtgaatt ttatcaatca aggtgctagc gtcacgtcca
    34981 ataccatcag cattaatttc catcatttgg ataataccat ctggatcaac gataaatgtt
    35041 ccacgttgag caagtccgtc ttcgccaagc acttcaaagg cttgtgaaat aaggtgtgaa
    35101 gggtcaccaa tcatagggta tgtgatagtg ccaaccacat ctgaatcatc atgccaagct
    35161 ttatgaacaa aatgagtatc agtagagaca gaataaactt ctacaccaag agatttcagt
    35221 gtttcgtatt gctcttgaag gtcaccgagt tcagttgggc aaacaaatga aaagtctgct
    35281 gggtagaaac aaaaaactgc ccattttcct ttaacgtctt catttgtaac agtgatgaat
    35341 tttccatcgt gataagcttg agctgaaaat tcagcaattt cttttccaat tagagacata
    35401 tagttgtcct ccttttttat agttttatac ttgtattata aaagaacaac aagattatgt
    35461 ctactcagaa acccttgatt ttactgggaa atcagcgatt attttctgac aattaagttg
    35521 tgaaataaat cacattaatt aaaatgattc taaataagct gaagatgccg ttaaatcaaa
    35581 ggttaattta attataatct acagaaaata taattgttat taaagcgttt acaaaaaact
    35641 tttttgattc cgttaaggca acttaatatt atggttacct aatcgctaat attgtaacct
    35701 attttggcat gagatcaggg aatggctgtt atattaattg gatatgtctt gtgcaaatag
    35761 tgcttgacgg atttgtggaa ttagggtaaa ataatctttg ttgtggcggt atagccaagt
    35821 ggtaaggcac ggctctgcaa aagcttgatc gtcggttcaa atccgtctac cgccttttat
    35881 atacaaaaca agccttgatt aacttcgtga aaacgttgtt aaatcaaggt ttttattttt
    35941 tgcggcagga taaaaaaaga gaaaaaaaga aataaagtcg gtcaaaagtc ggtcaaattt
    36001 cccgaagatg tcttttagag cctgattatt aggtatgaaa aatactatgg ccaaaattgg
    36061 taaacgattg caaataaagg tcagagttct taccccctta aacgatatgc gttaaaagtt
    36121 agttgaactg ccgtgcacct taacgataga cacggtgatg tgagagcgag tagagttgaa
    36181 tctctacttg tttaccttac catagtcagc tttataaagt aaccacccat tacaatgcaa
    36241 ctatattctt aatgttttta agcatgttat tgacaagtgt tataaaatgt gttactctta
    36301 gtgagtaaat tagtttttga gttagtattt gtgtttcaac atctttattt ctctctattt
    36361 ttacaaatga tttttagaaa ggaagtggat gtatatggca caaggtacag taaaatggtt
    36421 taatgctgaa aaaggttttg gttttatttc aactgaaaac ggtcaagatg tctttgcaca
    36481 tttttcagcc atccaaacta atggttttaa aacgttagaa gaaggacaaa aagtggcatt
    36541 tgacgtcgaa gaaggtcaac gtggacctca agcagtcaat atcactaaat tggcctaatc
    36601 tggttaaagt aactaaagag aagcagatgt tggaaacagc aactgctttt ttgatttcta
    36661 ccagttacct ttaatgatta agagttgcgt ttaaggtctt gtatttgtta taataatagt
    36721 cagttttagt caaaatgtat tgacgtggtg ctactaagtt tttagagtag cccgcaagca
    36781 aaggagtgac agcatgccaa ccaaaaatac ttcagacagc attgaagagt atattaaaga
    36841 attattagct aagtcaggaa tagcagaaat taagcggtcc atgctagcag attcgtttca
    36901 agttgtccca agtcagatca actatgtcat taaaacccgt tttacggaga gtcgaggcta
    36961 tgaagtagag agtaaacgag gtggtggagg ctatattcgt attgccaagg ttcatttttc
    37021 agacaaacac catctgattg gcaatttaat ggcaactatt gaagactgta tcagtgagca
    37081 agttttcacg gactcgatac aattgctttt tgacgaacac ttattgacag agcgtgaggg
    37141 aaatatcatt ttggctgtgg cgtctgatga tgttttagga actgatggtt caactattcg
    37201 tgcacgaatg ctctatcgct tattgcaacg aattgacaga aagggaagca attaatgatt
    37261 atgtattcaa cgaagatgca agacattttt agacaggcgc agttccaagc tgctcgcttt
    37321 gatagccatt gcctggaaac ttggcatgtt ttgttagcta tggtagctgt agataattct
    37381 ttagcaaata tgattttaag tgaatatgat gcccaagtcg ccatagaaga atatgaagct
    37441 gcagctattt tagccatggg caaaacccct aaagaacagt tgtctcgtgt agacttcaga
    37501 cctcaatcta aaactttgac taacttgtta gcttttgcgc aggctattag ccaaatcact
    37561 agggatcaag aagtcggctc tgagcatgtc ttatttgcta ttttattgaa tccagatatt
    37621 atggcgagtc gtttgttaga aatagctggc tatcagataa aagataacgg caatgggcag
    37681 ccgcgattag ctgacttgcg aaaagcaata gaacgtcatg caggttacag taaggaaatg
    37741 atcaaggcta ttcacgaact acgtaagcct aaaaaaacga aaacacaagg gaccttttca
    37801 gatatgatga agccaccaag tacagctggt gagttgagtg atttcacaag agatttgact
    37861 gaaatggcaa gacaaggttt gttagaatcg gtgattggac gtgaccaaga agtatctcgt
    37921 atgattcagg tactaagtcg taaaacgaaa aacaatcctg tcttggtggg tgatgcaggt
    37981 gttggtaaaa ctgcgcttgc ttatggcctt gctcaacgga ttgcaaatgg cgctattcct
    38041 tatgaactta aggagatgcg tgtcctagaa ttagacatga tgagtgtggt agcaggaacc
    38101 cgttttcgtg gggattttga agagcgcatg aatcaaatca ttgatgatat tgaagctgat
    38161 ggtcagatta ttctttttgt tgatgaacta catactatta tgggttctgg cagtggtatt
    38221 gacagtacac ttgatgcggc taacatttta aaaccagcat tatcgcgcgg cactcttcat
    38281 atggttggag caacaactca agaagaatac caaaaacata ttgaaaaaga tgcagctctt
    38341 tcgcgtcgtt ttgctaaaat attaattgaa gaacctaata cagaagatgc ttatcagatt
    38401 ttgatgggcc taaaattatc ttatgagacc taccataatg tctcgatatc aaatgaggca
    38461 gttaaaacag ctgtaaaaat ggcacaccgt tatttaacca gtaaaaatct ccctgattca
    38521 gctatcgatt tattagatga agctagtgct gctgtgcaaa acatggtgaa aaaatcagca
    38581 cctgagactt taacaccaat agaccaagct cttatcaatg gtgatatgaa aaaagtatct
    38641 cgcctcttag ctaaagaagc aaaaggtcag atgagaaaac caacaccggt gacagaagat
    38701 gatattttgg caaccttgag taagttatcg ggaattccac ttgaaaaact gacgcaagct
    38761 gatagtaaaa aatacctcaa tttagaaaaa gaactgcata agcgtgtgat tggtcaggat
    38821 gctgctgtta cggctatttc aagagccatt cgtcgcaatc agtcaggtat tcgaacagga
    38881 aaacgtccta ttggatcatt tatgtttctt ggcccaacag gagtaggtaa gacagaacta
    38941 gcaaaggccc ttgcagaagt tctctttgat gatgacgcag cgcttattcg ttttgatatg
    39001 tctgagtaca tggaaaaatt cgcagcgtct aggcttaatg gagcacctcc tggttatgtc
    39061 ggctatgatg aaggaggtga actgacacag aaagttagaa ataaacctta ttcagtcttg
    39121 ctttttgatg aagtggaaaa agcacatcct gatattttta acgtcctcct tcaagtatta
    39181 gatgatggta tattgactga tagtcgtggg cgtaaggtcg atttttcaaa tactattatt
    39241 atcatgacca gcaatcttgg cgcaacagcc ctgcgcgatg ataaaacggt cggttttggg
    39301 gtcaaaggca ttcaccaaga ccatcaagct atggagaaac gtattttaga agaattaaga
    39361 aaaacttacc gcccagaatt tatcaatcgt attgatgaaa aagtggtctt tcatagtctg
    39421 acccaagata acatgcgtga tgtggttaaa atcatggtac agcccctgat tactacattg
    39481 gcagaaaaag gtattaccct taaaattcag cctttggcct tgaaacattt gtccgaggtc
    39541 ggctatgatg agcatatggg ggcaagacca ttacgtcgaa cgctgcaaac tgagatagaa
    39601 gataagctat cagagcttat tctttctcga gaattgacaa gtgggcatac gctaaaaatt
    39661 ggattatcac atggcaaatt aacgtttcac atagcttaat ttccatataa aaggtggatc
    39721 atccttgatg tcaccaaaaa aagaggttat tcttacaaaa ataacttctt ttttcttgac
    39781 tatttttgac caagtgataa aatagaatat aaagttagca ctcgatgaac atgagtgcta
    39841 actttactaa agattctttt attggaggaa aatttcatgt taaaaccatt aggtgaccgt
    39901 gtggtcgtaa gatttgatga tgaaaaagag cagacagtcg gtggttttgt ccttgcaggc
    39961 actcataaag agtcaacacg aaaagcgaca gtcctcgctg tcagtgaaac gggtgttcgc
    40021 actattacag gtgatagcgt gctaccttca gtttcagtgg gtcaagaggt tttagttgaa
    40081 aatgggcatg atttagaagt aaccgtagat gatgagaaag tttctatcat ccgtgaatct
    40141 gacatcattg ctattgttac aaaataaaac gacaaatctc taaatagaaa gaaggattga
    40201 atatggcaaa agatattaaa ttttcagcag atgcgcgtgc tgccatggtg cgcggagttg
    40261 atatgttagc agataccgtc aaagtaacgc ttggtcctaa agggcgcaat gttgttcttg
    40321 aaaaagcttt tggttctccc ttaattacta atgacggggt aaccattgct aaagagatcg
    40381 aattagaaga tcattttgaa aacatgggag caaaattggt gtctgaagtg gcttctaaaa
    40441 ccaatgatat tgctggtgat gggacgacta ctgcaacagt tttgacacaa gccattgttc
    40501 atgaaggact aaaaaatgtg acagcaggtg ctaatccaat tggtatccgt cgaggcattg
    40561 aaacagcaac agcaacagct gttgaagcct tgaaagccgt tgctcaacct gtatctggca
    40621 aggaagctat tgctcaggtc gctgcagtat catcacgctc tgaaaaagtt ggagagtata
    40681 tctcagaagc tatggagcgt gtgggcaacg atggtgtgat taccatcgaa gaatctcgag
    40741 gtatggaaac agaacttgaa gtggttgaag gcatgcaatt tgaccgtggt tacctgtctc
    40801 aatacatggt cacagacaat gaaaaaatgg ttgcagacct tgaaaaccca tttatcttga
    40861 tcacggataa aaaagtgtca aacatccaag acattttgcc actacttgag gaagttctta
    40921 aaaccaaccg tccattactc attattgcag atgatgtgga tggtgaagcc cttccaaccc
    40981 ttgtcttgaa caagattcgt ggtactttca atgtggttgc tgtcaaagcg ccaggatttg
    41041 gtgatcgtcg taaagctatg cttgaagaca ttgctatctt gacaggtggt acagtgatta
    41101 cagaggatct aggacttgaa ttaaaagatg ctacaatgac agcccttgga caggctgcta
    41161 agattacagt tgataaagat agcacagtaa ttgttgaagg ttcaggaagt tcagaagcta
    41221 ttgctaaccg tattgcactg attaaatcgc aattagaaac aacaacttct gactttgacc
    41281 gtgaaaaact acaagaacgt ttggcgaaat tagctggtgg tgtagctgtt atcaaagtag
    41341 gagctccaac agagacagct ttaaaagaaa tgaaacttcg cattgaggat gctctaaatg
    41401 ccacacgtgc agccgttgaa gaaggtatcg ttgctggtgg tggaacagca cttattacgg
    41461 tgatcgaaaa agtagctgct cttgagcttg agggcgatga cgctactgga cgtaacattg
    41521 tgcttcgtgc tctagaagag cctgtacgtc aaattgcttt aaatgctggg tacgaaggct
    41581 ccgtagttat tgacaagttg aaaaacagcc ctgcaggaac aggatttaat gctgcaacag
    41641 gtgagtgggt tgatatgatt aaaacaggaa tcattgaccc tgtcaaagta acacgatcag
    41701 cgcttcaaaa tgcagcttct gtagctagtc ttattttgac aacagaagca gttgttgcta
    41761 ataagcctga accagctgcg ccagcgccag caatgccagc aggtatggat ccaggaatga
    41821 tgggcggctt ctaagctctc tatagcatta aggattatga aaaagaagtt gagactaaag
    41881 gtctgcttct tactatgaaa aaagaacaaa gattggattt cgactcatga tatcccatct
    41941 ttgttttttt agttgtgata taaaatgcta atctttgtgg ttacagactt ttaatgtatt
    42001 ggaaaataca ttgtttttct ttgtcatgtg ctatcatgga gtcaatgaaa agttattccc
    42061 atatatgggt gttggaggga aattttggag agatttttaa aacctttaga gaatcaaagg
    42121 gattacgatt aaaagatgtc gcaaaggctg gtgtatcaac ctctcagctg tcgcgtttcg
    42181 aaaaagggga gacggactta accatatcga catttatgct gattttggat gaaagtaaca
    42241 tgcccattaa tgagtttatg tatgctgtcc atgattttca tcgtgacaac acaattcaag
    42301 atagtgatgg cttgaagcag ttacttcatg ctcaactagc atcaactgat aagaaggaga
    42361 tgttcacgga tatcttttgg catatccagc atgtctaagt ctgctatggc atgctatttt
    42421 gggagtccct cacgcgactc tgtcagtagc tctccttcaa aggattcaca tagaaagtca
    42481 taatagacat agccctcttt ttttgtcagg ctggataaag ctagaaatgc cctttaattg
    42541 aaggtttaaa actgtcagat ctatctcttc ttttagttct cataaagccg cttcaaagaa
    42601 agattcgcag ggctcgactt taccttctgg ctggacccat cctgggaagt tatcgtgttg
    42661 tctatggagt cataaaatca tatcgttatg tttgacacac atattgcccc agtctctaac
    42721 cgttgctatc accagattca ccacctttta gtatagtata agaaaaaagg ggtgaaagag
    42781 gtttcttgtt gtttttttgg cttaaggttt ctttaagtct tatctagtat actaatgtca
    42841 ttccttaata ggataagtat ttggttgtag aagtatcgtc ttaaactaat gctatctcaa
    42901 cccgattccc atcttattca cccctcctat accgataagg tttcacaaag tgtgagacct
    42961 tattttttga cttcagctct agttatggag aaacttgttg atacaagctt ttttgggtta
    43021 tggtattatg atctattcaa gcctttcttg tttttgggtt tttctttaac caacttggct
    43081 tcaatagctt ttaagcgttt gaaggttttt tcagcgcgat ctagtgaaat cttatcagct
    43141 ttttcttgag cagctttggg gtcactgacg actaagtcac tgatttcttt ggaggttttg
    43201 tcttgttcgg ctatccaggt tttttccaag cctttcatct catcaatcac tttagttcca
    43261 aatggttttg gatgagcagc cacgagatca ttaatgtgtg acaccgtcca ataccatgac
    43321 ttatcgttat attgggtgct tttttcttga taggcttcat aggttctact gatattgcca
    43381 aggtatggca agtaaggggc attacgagga ctgccaatag atagccacat gacaccaccc
    43441 aattcagcag ggatgtcttt tttcagttgg aaaatatgag cttccatgac attaggattt
    43501 gatatgggat aagcataacc tttaacagct tttttggatt ttggtttacc cttaccgtct
    43561 aatgccatct gatcaagtgg tttaagatcc agcccctcaa aacgattacg ttgcagtttc
    43621 atcgcatctt ctagactgaa ggtcttatca gtgctttgga gcaactcata attgctgtct
    43681 ttataagtta cttttgaatc agggtcaagt gatttaatcc ctgagaaaga gcgagagcgg
    43741 ttggcatcgt ttagtggtgg attgtaagat ttggcaatat gaaatttacc atcaacttcg
    43801 gtataagatt tggctttttt agcgactttt tccacatctt ctgaggcaat cgtattttcc
    43861 ttatcgttga aatcgacatg tccgagatag aaggtatttg gaaaaacagc gtatttatca
    43921 tctgggaatt tgatggcaac gtattggtga ccagagagaa tttccatgta ccagatgccg
    43981 tctttgtcag ccaaggtaac aatatttcct tcagcagctc ctttttcagt gactatttta
    44041 gcgatcaatg cgacaccttc acgggctgtt ttgacacttg gtaaaatgac actggccata
    44101 gatgattccg ctaagccatt tttgacgtaa ggatcaattt tttggatggc atcattagct
    44161 gaagctgata ctgttgcaga catagagacg ccaaactcat taaaaccagc ttcgtcataa
    44221 acgcctttgt taggtgttac atcaggaatc gcactatagc gataagaatg ctcaggcaac
    44281 gggtactcaa aaccattaga gaggtctttc catttttcgc cagcaggatt gtcctttgct
    44341 agacgtacaa tgaaattttt attatggtgt ggctcaagat cttctgttcg accataaaga
    44401 agagaaccgt ctttggttaa atctttgcca ataataaaac cagtacaagc ataactaaca
    44461 ctttgaagcg aaaaggctgt cagaatactt aaaactccaa gtgatatttt tttgtttatc
    44521 atgcgaatcc tcctagtaaa cgataacgct ttcacagaca ttataaaatg ctctttttaa
    44581 aaattcaagt cttttatgaa gtgttttgaa attgttagct atctaaatat tatgagaatt
    44641 ctctcttgaa ttttggctta acggccccta aaaacgtata ggcagtgaac tatctaggtt
    44701 tcttcgaaaa tccattagcc atttatgata taatagtaaa gaaaaacata gaaagttggg
    44761 gtctgttgat gaccaaacca tttcatcata agaaactaaa acaaataact ataatagctg
    44821 caactagcct ttttttattc ctgatcggtg gtgcctttta ctactctaaa aatcattgta
    44881 ttaatgccta tcttaaagct cgatcagccc aatcaggacc agtttttgag aatatcaaag
    44941 catatctagt ctgggatgat actaatgagc agatcacaaa tgacgaggcg atgtatacta
    45001 agtttagaag gtatagtcaa aaagaattga ggcaaaaaaa gcaggattta aaagctgcta
    45061 gtcaagatag tgccgttcaa gtaaagtctg ttggtcgtcg tttttggatt tttcctgatt
    45121 atcggattgc cataaaaccc atggatttaa cgataaaaac gaatgtgcct caagcagacg
    45181 ttcttttaaa tcaaaaaaaa gttgctgttt ctgattcaga acagttctca gtcaagcttg
    45241 atcggctacc aacggcagaa tataccgcaa gtatcagagg caaacacaac gggcgaaaca
    45301 ttaaagtcaa caaatcatat gatggtgata atcccgtgct agatttgagt gtgtctttta
    45361 gaactttttt ggtaacaagc aatgctaagc aaggagatct ttacttcgat gataaccata
    45421 ttggcacatt aaaagatggt caattacaag tagaagatta ccctgttaca gaaaacgcac
    45481 aagcttatat gaaaacaaca ttcccagatg gtgagttaag atcacaaaaa tatgctctag
    45541 ctgatgttga agaaggagca accctggaga ttttagttac agatctttta gaagaggaca
    45601 aagcagggga gctattagta tcagcttttg atcagctgat gcactacctc agtacaggtc
    45661 aagactcatc taacttacgt agcgtctttg aggcagggtc tagcaacgca ttttataggg
    45721 gattgaaaga gtccataaaa gcgaaatttc agacagatac aagaaaagcc agccgtctca
    45781 atattccatc tatccttttg acaacaatga ctcaagtggg taaaacaact tacgtgcttg
    45841 atttcacagc tacctacgaa tttttgtatg acaactcaac agatcctgag cagcatacct
    45901 ctggacatat taatcaagac ttgactggaa aagtgacggt aaaaaaagtt ggacagcatt
    45961 accttatcag ccagtccggc tctaaaaata ttactgttgt taaagaggac aatcaactca
    46021 aagcgccatc tgtctttcct gagtctattt tgggaacgtg gacaggccaa gccaatggtt
    46081 tgagcatcca tatgtctcta gcatcagatg gaacaattac gactaaagtt gaagatcaaa
    46141 aaggcaaccg ttctaaagaa actcggacag ctaaaattag taaagttgaa gacaaaggca
    46201 atggttttta tctttatacg ccagatcctg gaagtgacat aagcgcctta gctccagaag
    46261 gaggattggg gggtgcaaat gtcaaatatg cttatggttt gaaaatatct ggtaaaacag
    46321 cctctccagt ggtgtggcag gcagcattaa cacatgaatt tgattatacc aagccacttt
    46381 cgggtgtaac tttgcaaaag caaccataaa ggatgcaaag ccacaaagaa cagtagaaac
    46441 aatgttatta ctcaaataca gtgctatgaa aacagataaa ctcaaaaggc taggagatta
    46501 tttcctagcc ttttgagcgt ttaagactgc gtcaaatgta gctgatttag cttgcacgca
    46561 aatgtcttct agggttagag ggtgtttcaa acgtaattga taagcatgga gcatcagtcg
    46621 agcacaatct ttttttccat tagaatacaa aggatcacca aataaaacat gaccttgatg
    46681 ggctaaatgg actcgaattt gatgagtccg tccagtttgt aattgacaat tgagtaagct
    46741 tgccgttttg tggaagtttt tgactagagt tacctcagta atagctttct tgccatttat
    46801 cggatcgaca acacgttttc tgcggtcatg tcgatgacga ccaattggat gatggtaagt
    46861 taccctagga ctgtctaagg atccgtgaac tagtgccaaa tattctcgat ggatatctcg
    46921 tttttctaaa agacgattaa ggattggtaa gataaaaggt gttttagcaa ataaaatggc
    46981 cccacttgtt tccttatcca gacgatgaac cacatagcag gtctgtcccg tgtaagcaga
    47041 cacgtggttg agcagcgcca actctgttgg accattgccg tgtgttttca tcccttcagg
    47101 tttgttaaca ataatgatgt gctcatcttc ataaaggcag gttacttttt cggcctgccc
    47161 catcacgata atcttttcag gataatcttc gtgatcgaaa aaaagtttga cctgatcacc
    47221 gtatttaaca caactttgcc agttaacagg atgaccatta ataagaacat gttttttagt
    47281 tctcagaaaa tgcctaattt tacggggaat taacaattgt tcctctaata atgctttgac
    47341 cgtcaattct tcaaagggat ttttcagtgt gacagtaaac ccctttgatg gtttatttgt
    47401 tgtcttcatt gagccagtta tggtattttt caacaaggtt gatagccatt tgggtggcaa
    47461 gagcagcaga gaaagcttca gtaccttcac gctcgtcatt agctacctcc aagcgagtct
    47521 catcgtaaat ggcttttaga ctttctgcgg ataaggtttc tttaaaagct tggaatttat
    47581 tcattggtta aacccgtcct tctatgttta atgtgagctc ctgattaatg caaggaattc
    47641 aatgctatta tagcatgttt ctaatcaaaa cgtgccactc aaaaaggtaa aaacgttatt
    47701 ttgctaatcc atataataag aaagtgtctt aaataagacc tcttgataga gtttgtaaca
    47761 aaaatgatat gaaactaggt gctaacgcag aaatagaaaa caactaacgt gttgcgaagt
    47821 tggaacagtc ataaaaaata gcaagataaa acttatcaac acatagttac ctaagacgat
    47881 gagtcaaaca ggtatgttct ttcaaaacaa ccccatgagg gtgaacgacg tatcggaaaa
    47941 atacggatta gtttggcctt at
//
LOCUS       DAUCWU010000008        39090 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUCWU010000008 DAUCWU010000000
VERSION     DAUCWU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39090
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.008"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(107..1243)
                     /gene="dnaJ"
                     /locus_tag="VGY24_000419"
     CDS             complement(107..1243)
                     /gene="dnaJ"
                     /locus_tag="VGY24_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ4283428.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(1524..3350)
                     /gene="dnaK"
                     /locus_tag="VGY24_000420"
     CDS             complement(1524..3350)
                     /gene="dnaK"
                     /locus_tag="VGY24_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ4283429.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(3531..4103)
                     /gene="grpE"
                     /locus_tag="VGY24_000421"
     CDS             complement(3531..4103)
                     /gene="grpE"
                     /locus_tag="VGY24_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ4283430.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(4106..5140)
                     /gene="hrcA"
                     /locus_tag="VGY24_000422"
     CDS             complement(4106..5140)
                     /gene="hrcA"
                     /locus_tag="VGY24_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ4283431.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKTFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(5275..5844)
                     /locus_tag="VGY24_000423"
     CDS             complement(5275..5844)
                     /locus_tag="VGY24_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4283432.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(5841..6584)
                     /locus_tag="VGY24_000424"
     CDS             complement(5841..6584)
                     /locus_tag="VGY24_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ4283433.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDSQEHLISGYRSVDYQTELYQSYIKKEMANDPTLTQEAAEALVQTYSQPPGT
                     SEHQTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(6574..7281)
                     /locus_tag="VGY24_000425"
     CDS             complement(6574..7281)
                     /locus_tag="VGY24_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ4283434.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(7673..7912)
                     /locus_tag="VGY24_000426"
     CDS             complement(7673..7912)
                     /locus_tag="VGY24_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ4283435.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(8131..8346)
                     /locus_tag="VGY24_000427"
     CDS             complement(8131..8346)
                     /locus_tag="VGY24_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283436.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNYHVCLHYY"
     gene            complement(8343..9782)
                     /gene="gatB"
                     /locus_tag="VGY24_000428"
     CDS             complement(8343..9782)
                     /gene="gatB"
                     /locus_tag="VGY24_000428"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ4283437.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNVEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(9782..11248)
                     /gene="gatA"
                     /locus_tag="VGY24_000429"
     CDS             complement(9782..11248)
                     /gene="gatA"
                     /locus_tag="VGY24_000429"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ4283438.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASRDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(11248..11550)
                     /gene="gatC"
                     /locus_tag="VGY24_000430"
     CDS             complement(11248..11550)
                     /gene="gatC"
                     /locus_tag="VGY24_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ4283439.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(11782..12117)
                     /locus_tag="VGY24_000431"
     CDS             complement(11782..12117)
                     /locus_tag="VGY24_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
                     /protein_id="HEQ4283440.1"
                     /translation="MKDRYFFCDVVGKYLEEYVDRGIFDKEPFQTFDQKGIGRLFSTA
                     VSLGRSVKSKLKLGICGEHGGDPASIAFCHSQGLTYVSCSPFRVPLTRLAAAQAAIKA
                     SGHSLTQDK"
     gene            complement(12561..13115)
                     /locus_tag="VGY24_000432"
     CDS             complement(12561..13115)
                     /locus_tag="VGY24_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ4283441.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFNQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSHMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(13262..14044)
                     /gene="codY"
                     /locus_tag="VGY24_000433"
     CDS             complement(13262..14044)
                     /gene="codY"
                     /locus_tag="VGY24_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ4283442.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(14262..15476)
                     /locus_tag="VGY24_000434"
     CDS             complement(14262..15476)
                     /locus_tag="VGY24_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ4283443.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            15707..16159
                     /locus_tag="VGY24_000435"
     CDS             15707..16159
                     /locus_tag="VGY24_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ4283444.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNTKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(16282..17670)
                     /locus_tag="VGY24_000436"
     CDS             complement(16282..17670)
                     /locus_tag="VGY24_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4283445.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGVAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            17742..18707
                     /locus_tag="VGY24_000437"
     CDS             17742..18707
                     /locus_tag="VGY24_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ4283446.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRTRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(19050..19754)
                     /locus_tag="VGY24_000438"
     CDS             complement(19050..19754)
                     /locus_tag="VGY24_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283447.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(19767..20669)
                     /locus_tag="VGY24_000439"
     CDS             complement(19767..20669)
                     /locus_tag="VGY24_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283448.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            complement(20961..22976)
                     /gene="recG"
                     /locus_tag="VGY24_000440"
     CDS             complement(20961..22976)
                     /gene="recG"
                     /locus_tag="VGY24_000440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ4283449.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKKEVYD"
     gene            complement(23351..24805)
                     /locus_tag="VGY24_000441"
     CDS             complement(23351..24805)
                     /locus_tag="VGY24_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283450.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGPSGSGKSTFLKFLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(24742..25422)
                     /locus_tag="VGY24_000442"
     CDS             complement(24742..25422)
                     /locus_tag="VGY24_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ4283451.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(25419..26012)
                     /locus_tag="VGY24_000443"
     CDS             complement(25419..26012)
                     /locus_tag="VGY24_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ4283452.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(26009..27679)
                     /locus_tag="VGY24_000444"
     CDS             complement(26009..27679)
                     /locus_tag="VGY24_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283453.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            complement(27672..29435)
                     /locus_tag="VGY24_000445"
     CDS             complement(27672..29435)
                     /locus_tag="VGY24_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ4283454.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQS
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQEIINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLSTGQPACLGKPCDLYQ
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(29432..30268)
                     /locus_tag="VGY24_000446"
     CDS             complement(29432..30268)
                     /locus_tag="VGY24_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283455.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(30265..31287)
                     /locus_tag="VGY24_000447"
     CDS             complement(30265..31287)
                     /locus_tag="VGY24_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ4283456.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYEAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(31289..32173)
                     /gene="isdE"
                     /locus_tag="VGY24_000448"
     CDS             complement(31289..32173)
                     /gene="isdE"
                     /locus_tag="VGY24_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ4283457.1"
                     /translation="MIKRCKGIGLALLAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDRMGDHP"
     gene            complement(32157..33032)
                     /locus_tag="VGY24_000449"
     CDS             complement(32157..33032)
                     /locus_tag="VGY24_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ4283458.1"
                     /translation="MTKVVIKQLLQVILVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(33229..37056)
                     /gene="shr"
                     /locus_tag="VGY24_000450"
     CDS             complement(33229..37056)
                     /gene="shr"
                     /locus_tag="VGY24_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ4283459.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEALVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLTEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFVENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKSSNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVEKGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVMSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(37546..39063)
                     /locus_tag="VGY24_000451"
     CDS             complement(37546..39063)
                     /locus_tag="VGY24_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4283460.1"
                     /translation="MLLSLLAPTAESMTVLAQDVMLETHKATTNETSDSSSKEENNKN
                     AAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKAEAENQVDDKS
                     TDHSKPTDHSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPIQSLS
                     PYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIA
                     YDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDP
                     TQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTG
                     SGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLDYQAS
                     SYAWGQCTWYVYNRAKELGYRFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAG
                     ADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
BASE COUNT        12042 a         8115 c         6706 g        12227 t
ORIGIN      
        1 gaaaaaaacg atatctataa aagaaaacgt tttctctgac tgttttgaca taaaaaaaaa
       61 cgtataactt cgggaaaatt atcctgagat tatacgtttg acatctttaa atatcctcta
      121 aggcatcttt cactttatca aaaaaacctt ttttctttgg atgtaacatt ttttcaccac
      181 tcgcttctgc aaatgcttgc aaggcttccc tttgagcgtc attgagtttg gttggggtca
      241 caatattaac ggtaacatgt tgatcccctt gacctccacc acggagtttt ggtgctcctt
      301 tgcctttgag gcggaaggtt ttacctgttt gtgtcccagc agggatagcc atctcaacat
      361 cgccatgaac cgttggaatt tcaacagtat ctcctaaggc tgcttgagta aaagaaatat
      421 ctaagttgta gtaaatagtg gaaccattgc gttcaaattg cttacttggt aagacattca
      481 aaataacaaa taaatcacca taaggtccgc cattaaagcc agcttctcct tgaccttgta
      541 aacggatttg ctgaccagtc tctacaccag ctggaatctt aacagacact ttatgagctt
      601 gcttttcatg accagtaccg tgacaggttt gacagggttc tttaatttcc ttaccactac
      661 catgacaaat atcgcaggtg acttgtcgac gcatcatacc aagtggtgtt tgggtgtcaa
      721 ttgtcattac acctgaacca tggcacttac gacaagttac tggagcggta ccaggttttg
      781 cacctgagcc taaacaagtg ccgcaggttg cttcgcgatt atagctaact tctttttcaa
      841 caccaaatac agcctcttca aaagataaat taacacgata ttgcaaatcg tcaccttgac
      901 gaggggcatt tgggtttcgg cttccaccgc caccaaagaa gcttgagaag atatcttcaa
      961 aaccaccaaa gccacctccg tcaaaaccac caaagccgcc tgctccgcca ccaaagccgc
     1021 cttgagcacc tgcagcgccg tattgatcat aagcagcacg cttttgtgaa tcacttaagg
     1081 tttcgtaagc ttcttgaaca tccttatact tttgctccgc tccagcttct ttattgatat
     1141 ctggatgata tttcttagac atttttcgat aagctttttt gatatcgtcc tgagaagcgt
     1201 ccttagaaac ccccagacga tcgtaatatt ctgtattgtt cataatgtaa gataccaagc
     1261 cgtaaaacga ctcaatgaaa aataggaaat ccgacaaaga tactctcgta tctaggaggt
     1321 atttctcttt tttcgcagag tcgtaggcgt gttcgaatcc gcaaaagagt ctttacacgc
     1381 tttagcctgt gttcgaataa tcgaacttgt attcctttct taaaatattt tgacgtcagc
     1441 atttttacat tttatttagc caaaaacaga ggtttggtac aacctctgaa ttcggatatt
     1501 aatgttacta gataactaaa tcattacttt tctgtaaatt cgccatctac aacatcatca
     1561 ttatttgctg aatcattagc ttgtgcacct tctgcacctt gtgctgcttg ttgagctgct
     1621 gcagcttgct cgtacatttt aacagccaaa gcttgcgctt tttcatttaa tgcttcaagt
     1681 ttagctttca tgtcgtcaag gttgccagat tcttgcgcag cttttaactc gtcaagagct
     1741 gattgcgctg catcgcgttc tgtatcaaag cctttacctt cagtttcttt gattgttttt
     1801 tcagtagcaa agatagcttg gtcaacttcg tttttaaggt caacttcttc tttacgtttc
     1861 gcatcggctt cggcattagc ttcagcgtct ttcatcatgc gatcaatttc ttcttcagaa
     1921 agtccgtcgt ttgatttgat aacgatgtgt tgttcctttt gcgtaccaag gtctttagct
     1981 tttacagaaa caataccgtt tttatcgata tcaaatgtta cttcaatttg tgggattcca
     2041 cgaggtgcag ctgggatatc agtcaattgg aagcgaccaa gagtcttgtt atctgctgcc
     2101 attgggcgtt caccttgaag aacatggata tcaacggctg gttggttgtc tgctgctgtt
     2161 gagaagactt gtgatttaga tgttgggata gttgtattgc ggtcgatcaa tttagtgaag
     2221 acaccaccca ttgtttcaat accaagtgac aatggtgtta cgtcaagaag gacaacgtct
     2281 ttcacatccc cagtgataac accaccttgg atagcagcac ccatagcaac cacttcatca
     2341 gggtttacag atttatttgg ttctttacca gtttcagctt ttacagcttc gacaactgct
     2401 gggatacgag ttgatccacc aacaaggata acttcatcaa tttctgacaa tgacaatcct
     2461 gcatctgaaa gagcttgacg aactggagtt ttcgtacgtt caacaaggtc acgagtgaga
     2521 tcgtcaaatt tagcacgaga taagctcatc tccaagtgaa gaggaccagc agaaccagca
     2581 gtgatgaacg gtaatgaaat ttgtgtttgt gtcacacctg acaaatcttt tttagctttc
     2641 tcagcagcat ctttcaagcg ttgaagagcc attttgtctt gagaaagatc aataccattt
     2701 tctttcttga attcttctac caagaaatca ataattttct ggtcaaagtc gtcaccacca
     2761 agtttgttat cacctgctgt tgcaagaacg tcgaagacac catcacctaa ttcaaggatt
     2821 gatacgtcaa atgtaccacc accaaggtca aaaactaaga ttttttcatc cttgtcagtc
     2881 ttgtccatac cataagcaag tgcggctgct gttggttcgt taacgatacg ttctacttca
     2941 agacctgcaa ttttaccagc gtctttagtt gcctgacgtt gtgcatcgtt gaagtaagct
     3001 ggaacagtaa taactgcttt ttctactttt tctccaagat agtcttcagc ataaccttta
     3061 aggtattgaa gaatcattgc tgaaatttct tgaggagtat attctttacc atttgcagaa
     3121 actttttcag aagttcccat ctttgatttg atagagatta ttgtttctgg gtttgtcact
     3181 gcttgacgtt ttgcagcatc acccacgata atttcaccat ttttgaatga tactactgaa
     3241 ggagttgtac gattgccttc tgggttagca atgatttttg attcagtccc ttcaagaact
     3301 gctactgctg agtttgttgt acctaagtca ataccaataa ttttagacat gttaatacct
     3361 cttattttat aatactttat tactttttat ttcaatttat cttagtaagc actcgaatta
     3421 ttttacttcc aattctaagc aatcactata gtttaatcag caagcgaaat agcttctaac
     3481 ttgctatcta gtttactgtc attacggaca aaaacgaaaa ctcatttacc ttagttataa
     3541 acaactacca tcgctggtct tagcagacgc tcgtgcaatt tatatccctt ttgaaagact
     3601 tctgcaatgc tatcggctgg atgttcatca tcggctggga gggtctgaac tgccatatga
     3661 aagttgtggt caaacgagtc aacttctact tcttctacgc cttcttcctt tagtgcttga
     3721 ataaggctat cacgagtcat ttctaaacct tttttaacat catctgtcaa cccttcaaca
     3781 gccaaggcac gttctagatt gtcaagcgat ggtaagattg cttttgccag atcctgagaa
     3841 cgatagcgtt gaagttgttg acgttcttca ctagagcgac gctgtatatt ctgcatttct
     3901 gcatgagcac gcaagtattt attttcaaac tcatcagcac gctcgtttgc gagttctaac
     3961 tctgattttt caggagtttc ttcaataact tcctcaactg tctcttcagt ttcagtggct
     4021 tccacttctt cttgtaaatc gtctttttta atttcttctg acactgagtg cctcctttta
     4081 aataacttat tgaatactgc cattatcagt gaacttcata atggttagaa tttaggtatc
     4141 gataataatc acctaattta gcagccaata cctttccaat aatgtttacc aaactaacag
     4201 acctacgata atccatatca ataggaccaa tcaaactcag gagtccaaac ccacggtaag
     4261 gaatcaaaaa tttgtgagtc aaaactgaca catctgcaag tgcggcttct tgactatctg
     4321 ccacttggac agaagccatc tcaccttctt tcagactttg cctcaaagaa attgctactt
     4381 gctgttcatt atccaaaaac tgatacgttg atagatctga atacgttaaa gaatttacct
     4441 tgccagcaac aaataccgtt tccaaaaaca gctctgaaaa aacataatca aatagttgta
     4501 aaacattatc cgtagtcaca aaatactttt gaacgatctg aggaatttct gtcctcaact
     4561 tataatgaat atctatgaca ctactatcca gcaagcgctc ctctacgata gctttaaatg
     4621 ctatcaagtc acgtgtcaaa aagtttctag gaatagcaaa ctgcactgtc actggcttag
     4681 attcatctaa agtcatgact gctagagcat catggttgct aagctgaaca acatcaaacc
     4741 ctgttaaacg ttgacgagct ggctccacat ctaaaataac ggatgtatag cccgtcatct
     4801 ccgctaaaat gtgactagct ttttgaagca tgtcttctaa tttgaatgct tcaaaatcaa
     4861 aagtttttat gacatgataa atatcttgct catcaatact atctagcctg agggagtgct
     4921 ctacaaaata cttaaaacca gctggacttg gcatccgtcc actagaagtg tgcgcttttt
     4981 ccaacaatcc caacttctct agttttgcca tatcattacg aatagtggcg ctactagaat
     5041 caatcgttct ctgtaaagct ttagacccta ctggttcatg agtttgtgta aatagctcaa
     5101 cgattaaatt caaaatatca ttttgacgtt gtgtaatcac aaaccaacca ctccctttcg
     5161 tttagcactc tatttagtag actgctaatt tataattcta ttatactctt tatgagagac
     5221 atgtcaagaa aaaaactcaa aaaaattagc actcttttat aaagagtgct aatttcagtc
     5281 atagtttgtt aaatcgttac tttcaatgac ctgaatcagt cgcctagcat aggttttatc
     5341 agtataatgc aaatactttt gtaaactttt agctggtgct ttataccctt tttctgtagc
     5401 aagattacga tacaaacgct tatcgacttc tttgccctgc cgaagcattc gtaaataatc
     5461 tctaattgca ctagcgtcat ctttataaat agcatatctc acgttttggt tggtttgttt
     5521 aggggacttt agggtaatgg ctgcttgtcc tggagttgct ttcttgctaa aaagattatg
     5581 atacttagac gctagtaatg tttttccatc atgggtttcc aaaagaattt gagcaatcaa
     5641 aatagatggt ctaatgccat aatattttgc caatggttta acttctttac ccatttttgt
     5701 aataaattgt ttttggctgt agttcaccct tacttcttta tctgcactag ctatcctttg
     5761 gctactcagt aagggacaaa caatcagcaa taaaaagcaa gctaggagcg ttaaaaaata
     5821 cggaaacttt agtcgttttc tcatcgtttc tcctttaaag cagcaatgta ctcttccaac
     5881 gttaggttgt gctgagccat ataacgagca gacgccttgc ctacatagcg gtaatgccaa
     5941 tcctcataat caacccctgt actagtcttt tttccttctg gaaagcgcaa gacaaaccca
     6001 taatcaggtg caattttttg aactgcttta gataccgatg ggtcactggc atttaaagta
     6061 tctacagtgc tcatgtcaat agcaagacca gtttgatgct cactagtccc tggcggctgt
     6121 gaataggttt gcaccaaagc ctccgcagct tcttgtgtca atgttggatc gttagccatc
     6181 tcttttttaa tataagattg gtaaagctct gtttggtagt ctacggaacg gtacccagag
     6241 ataagatgtt cttgcgagtc gatagcttgt gctgctgcta aaaagtcact agtggcttgc
     6301 tcaatgcgtt tatccacaga aataccatta atgtcagcta attcaggact catttctttt
     6361 gtaatgtgat cgcggtttac taaaaccaac tcccaatctt caacagatac cttaggtaaa
     6421 gctcgtaaac ggtccgtttt tttgatatct ttttgttgga tttctttttt attcaactga
     6481 gttggtgtta ctgactcttc tggttttata aagaggtaaa gccctcctaa aaaaacaata
     6541 acaattagca attgcactaa atagtttaac gaattatatt tcatctgacc gttcttctat
     6601 tatttcacga ccgcggtagc ggtaactcat atctccaata gtctttatcg taaattggcc
     6661 atcatcaaat tcaacaactg taacgctccc attatcaata tactgtttgt ctcgatctgg
     6721 atcaatcaac cacagaaacg ttccaattgt cataccatgg ctaaccacaa gagcatttcc
     6781 gccgccagca ttttgaatgg acaaggcaat agcttcaaac ccctcccaaa tgcgcttacg
     6841 aagaacttcc catggttctg cccaattagc ggtatctact tccacgatgc tttctgccag
     6901 ttcagaatag ggaacatcac gtaggttatc acgattttca aaggcttttg ttcttggaag
     6961 gacacctaga aataattctg aatcataagt cccttctaga ctcccaaaac accactcacg
     7021 aatacgatta tctttagtat aaggcaaaaa ttcgttttct gattctctta aaataatttc
     7081 tattgtctgc atggttcgac ctgaatcact tgaaaacgca gccttaaatg gtatagttgc
     7141 atcttttaaa cctaggccaa gttctcgaat accttcttct cctttttttg taagaggtgt
     7201 gtcactccaa ccctgagcgc gaccaatggt attaaacatc gtttttccat gtctggcaat
     7261 atacaacctt gtttttgtca tttcaacctc ctaaactgtc ttattaatta ttatagcatt
     7321 taaagaaaac acttgcaaaa cacttcctga tagctatttg agatcaaata gaccactgac
     7381 ttattgtcta ttttattaaa aataatgcta ttgctacaat tcaaatcatc acataaattt
     7441 ccaaacgatt acatttgtgt gtttcgctca aatatatata acaacagact cacactacaa
     7501 ctaaactgac gttacaaaat aatgtcaaaa cagtaacaaa atctaactat ttgtcaagaa
     7561 aatgatatta aattttcaca caactatctt ataatgagga tgcaaccaat aaaaagataa
     7621 gacattttat gttgcaaaaa aggctgcctg gaaacttcca tgcagccttt ttttaatacc
     7681 tttttccaaa aatagctgaa acaagtacaa ttacaattgc tgctcccaaa atggacggga
     7741 aaatagccat acctgccaac tgaattcccc aatccccaaa aagcctttgc ccaacagctg
     7801 accctatgag acctgcgata atattagtaa aacaacccat ggactggctt ttttcagtca
     7861 ttttacctgc tatcatgccg ataaaggcac ctacaattaa agaccatatc atcgttaaca
     7921 cctcctttct tgtaaaatcc ttttagaaga actgctatcc attaaatgtg cttaacttta
     7981 gttgattcct ttaccttctt ctttagtaag tgtttccgtg cctgagtaaa atagaaaaaa
     8041 caattccttc tattaagata gatagaccga gaacgactag aagtcttcac acactaaata
     8101 acttaacaaa ctttatcaca cagctaatcg ctaataatag tgtagacaaa cgtgatagtt
     8161 cattaaatac ccgtcagaaa ctcctgaagg ttctaaagca atacctgtca gtcttatttt
     8221 agcatagatg atggtctttt tataggcggt cactaacatg acatacaaaa aggcagcctg
     8281 tgaaagaccg accgcctttt tttcagagac aaaatacttt aacttattgc tatcagcaac
     8341 tatcaatctc gcaatttttg caattcttgt gccaataatt gttgagcgac ttgtggatta
     8401 gcttgacctt tggttgcctt catcaagaag cctgtgaaag ccttatcagc attacgtttg
     8461 cctgatttga aatcagctac tgcagcttca ttatcagcaa agacttggtg aataattggt
     8521 accaaaacag cgggatcaga aatctgaaca agtcctgcct tttcgacgta ggcacgtgct
     8581 gagccaccgt ttttagccaa atgcacaaag accttcttag ccattttaga agagattgta
     8641 ccatcagcaa tgatagcaat catctccacc aaattttctg gtgtcagagc gatttcttca
     8701 atggttttgc cttctacatt taagaattgg gcaacttctc cttgcaacca gttagagact
     8761 tgcttggcat cgccaccaag acttactgca gtttcaaaga aatctgacaa ggctttggta
     8821 gctgttagct ggctagcatc ataagctgag agaccaagtt cctcttcgta tttagcacga
     8881 cgttgagctg ggaattgagg taattgagca cgcatctcgt caatccaagc atcatcaatc
     8941 tcatacaagg gtaggtctgg ttctgggaaa taacgatagt ccgctgcccc ttctttgacc
     9001 cgcatcaaaa tagtcccctt attcgcttcg tcatagcggc gcgtttcttg gcgtatcacc
     9061 ccacctgaac gcaagagttt ggcttgacgt tccacctcaa attcaagccc tttacggaca
     9121 tttgagaagg agttcaagtt tttcaattcg gtctttgttc caaattgttc ttgcccgtaa
     9181 ggacgaaggg aaatattggc atccacccgc atagagcctt cttccatctt gacgtcagaa
     9241 atgcctgtgt actggatgat ttctttcaga gctgtcaaat aagcataggc ttcttcagga
     9301 gaacgcatgt cggcttctga cacgatttca atcaaaggaa ctccttggcg gttaaggtcc
     9361 acataagaat aaccatctgt gccatgagtg tttttaccag catcttcttc caagtgtgcg
     9421 cgttcaatac gaattttctt ggttgagccg tcttccaatt taatctcaat ccatccgttg
     9481 tagccaatcg gctcgtcaaa ctgtgaaatt tgataagctt taggattatc tgggtagaaa
     9541 taatttttgc ggtcgaaatg catctcttta tggatgtcca tattgagggc aagcgcagct
     9601 tttatgcccg catcaataac gcctttgttc atgactggta agacccctgg aaaagaccaa
     9661 tcaatcacat tcgtgttggc atttgggtcc tcaccaaagt gagctgacga aggtgagaaa
     9721 attttggaat tggtatttaa ttccacatgg acttcaagcc caataatggt ttcaaaattc
     9781 attacttgtc acctccaaaa ataatcggtt gttgcttgtg ataatctgtc accgcttcaa
     9841 aggctgctgc cgcttgatag atggtttctt cagcgtattt cggaccaatt aattgaagcc
     9901 ctactggcaa gccatcgacg aatcctgctg gaatggaaat tcctggcaga ccagccaagt
     9961 taactggaat ggtcaataag tcagccaaat acatggcaac aggatcgtgg ttgagtgtgt
    10021 ctaagccaaa agcaaccgtc ggtgttgttg ggcctaaaat aagatcataa tccgcaaaga
    10081 ccttgtcaaa atcttcaata atcaaggtac gcacttgacc ggcctttttg aaataagcgt
    10141 cataataacc tgatgacaaa ctaaaggtcc caagcatgat acgacgttta acctcatcac
    10201 caaagccttg gctacgagtg ttaacataga tttcatccaa gtttttagca tcgtcagcac
    10261 ggaatccata acgaatgcca tcaaaacgtt gtaagtttga ggacgcttct gacgaagcaa
    10321 taatgtaata gacagccaca ccatatttgc tatgtggaag actaacttcc tcaacagtag
    10381 ctcctaaggc ttcaaattgt ttagctgccg caagaaccgt ttccttaatc tcaggatcaa
    10441 tcccttcgcc aagatactct ttaggaaggg caatcttcat gcctttaatg tcacgaccaa
    10501 tcttagatgt gtagtctgca atgcggacag gcgctgaagt ggcatccttg acatctctac
    10561 ttgcaatcac atttaataat tgggcgtttt ctttaactgt cggagcaaaa ggtccaatct
    10621 gatcaagtga acttccaaaa gcaatgagac catagcgaga aacagtgcca taggttggtt
    10681 tcaaaccgac aacaccatta aatgcagctg gctgccgaat agaaccacca gtgtctgaac
    10741 caagagataa acggacttgt cctgaagcga ctgctgttgc cgaaccacct gatgagccac
    10801 caggtacttt tgtgtggtcc caagcatttt tcgttttctt gaaataagag gtttctgttg
    10861 aaccacccat ggcaaattca tccatgttgg tttttccaat gacaatcatg tctttagcgt
    10921 aggcatttgc tactgatgtg gcatcaaaaa tcggctcgta gttatacaac atttttgaag
    10981 cggctgtggt taggatgccc ttggttgaga tattatcctt aacagctagt ggaatacccg
    11041 acatgaggtt atcagcatca atacctttgg catcaattgc cgcagcttgt ttaagagcag
    11101 cttcttctga aacagtgata aacgacccga cagcctcctc acgagacttg atgtcttcaa
    11161 gtgttttttg agtcaattcg gttgcggaaa tctccttagc tacaaggagg tcatgcaact
    11221 cttcaatggt tttatgatta aaagacatta ggcatctcct ccgtcatcca aaatagcagg
    11281 taccttgata aagtgatttt ctttttcagg aacattttta aagagcaagg cacggtctgt
    11341 tccttcttca gcgacatctt gacgcatcac atttttctta tctgccatgg ttgttgtaat
    11401 ggccacacct tcggtatcaa cttcgttcag caattcaacc atgtcaacga ttttagatag
    11461 ggtcgttgca aaggttgttg tctcactttc tgagaatgat aattttgata gcttcgcgac
    11521 atggcgaact tcttcttcag aaattttcat gatttctccc atccaagatg acaagaagta
    11581 ttccagttcc aaagaacagc tctgctattc tctaaaggta gcggaaaaac ttctcacatc
    11641 atttttacat agtctcaatt attatatcac aattgttccc atttaccata ggtgactaat
    11701 gcaattctag aaagccatcc agcaaaaaac ttcctgacta aggtcagaaa accttagtca
    11761 ggaagtgtga caaactaaac tctatttgtc ttgggtaaga ctgtggcctg aagctttgat
    11821 ggcagcctga gcagccgcca agcgagtaag cggcactcta aatggcgaac aagaaacgta
    11881 ggtcaggcct tggctgtggc aaaaggcaat ggaagcagga tcgccaccat gttccccgca
    11941 aatgccaagt ttcaacttag acttaaccga acgacctaat gaaacagcgg tactaaagag
    12001 acggccaatc cctttctgat caaaggtctg gaacggctcc ttatcaaaaa tacccctgtc
    12061 aacgtattct tccaagtatt ttcctacaac atcacagaaa aagtaacggt ctttcaagtc
    12121 ggtaatatgt ttacgaaggt aataaagggg tgctttattc taatttacca aatcctcaac
    12181 gattgtgaca acatgtgatg tgataagcct aagataaaaa tggtacagac tctagcattc
    12241 aagagtttga agctttattt atattctaat gctgaaaaga ctagagtttg tctaatggct
    12301 gtgagacgat cctttaaacc atcaactcaa atcatttctc aaacagctaa aatcctgtct
    12361 tcttcaaaaa aacatgtgtt ctatctgaca cagtccaaag tttagttgcc ttttagacag
    12421 cacaagaatc atcaggacta attctgacca gcatgtcaca tacctcatct acctaaccca
    12481 tacatacggt aaaaccacat agagcaatga ttacggttac caaactctta gtcactgaat
    12541 caatagtttg gtaggctctt ttagtaatcg ttaataagct tagcaccaag aacctgctca
    12601 aagtgagcca aagaccactc ataagattct tttgtaaggc tagcgacagc cgatttaaca
    12661 atctctaatt gataaccaag atgataagcg tcaatagcag tatgtaagac acagatatca
    12721 gataaaactc ctgttaacac taattgagtg atacctcttt ctcgtagtcg aatatctaaa
    12781 tcagtccccg aaaaagctga ataataacgc ttatcaatcc aaaaaactct gggatgttgc
    12841 ttcatatgac tgtaaacttc agctagtgga ccataaagat ggcgtccagt agttcctttg
    12901 atattatgtg ctgcaaatag cttactctca ggatgccaag gatcattttg atcatgacaa
    12961 tcaatagcaa aaaaaatata gtctccttga ttaaatgccg ttttagttac ctctgctatt
    13021 ttagtggcaa tagcctgagc agatttacct gcacttaatt taccatcatc tgctacaaaa
    13081 tcatttgtgt aatcaattga aatcaatgct ctcatcacta tctcctaaac agggaaacct
    13141 ctccccttat ctcttatata tggcattgta tcagttttta ttacaggtat caagacatct
    13201 atttgcatct accattaaca gcaaaaaaca tctcttttca agagatgttg tctttttcta
    13261 tttaaaattc ttttaattta gcaaaaatac cttcgttgat aactttgagg tatgtccctt
    13321 tcataccaag agaacgactt tcaataatcc ctgcactttc taatttacgc agagcattga
    13381 caataacaga acgggtaata ccaatacgat cagcaataac agaagctgtc aatcgtcctt
    13441 cattaccatc taactctcca agaattgctg caacagcttt catttctgaa taagaaagcg
    13501 tattaattgc catgttgacc gctgtttgtt tacggatggt gtcttctaag ttttctgtct
    13561 gaagatttaa taattgaatc cccacaacag tacttgagat ctcaaccaaa atcaaatcat
    13621 catcactaaa ctcattgtca ttacgccaga taatgagtga tccaaggcgc atccctccac
    13681 cataaatcgg cgcaatagtc gttagacctc ctggataagt gtctttagac tcaacaggga
    13741 atatagtcaa ctcattttcg acagaaaggt tagcttctgt atcgtaaact cggctagcag
    13801 cctttacata agtatctgga aattgtttag cttcaaaaaa ttcctcaaca cgatcagtgt
    13861 tagttttata tttcatggca taaccaagca atgtgccgcc gccattgata atacaagcat
    13921 tgcagtcaat gatatctgct aggcgagatg ccattgtgtt atacggtaat tctgtttcta
    13981 ggctatctac ggaacgctgc aaaatagatg tgattttacg agttttttct aataagttag
    14041 gcattttttt ccttttcctt tgaattattt gcgctttcat tataacaaag ctaactcgct
    14101 tttgcaagga attatcagaa aattttctat tcttactaca attccaacta ttgatgcctt
    14161 attatcactt catttaaagg agataagcac tagcttgtta gagcataggg tcacattatt
    14221 ttaagaaacg ttatttttat taatcaaaga tgagcattgc attaacgtct atactggtat
    14281 aagacacgtg taatcttttc ttggacatct tctaattctt ctacacttgg taggtaaaca
    14341 atacgaaagt gatctggatc tttccagtta aaaccacgtc catgtaccaa catcactttt
    14401 tcttgcttta ataactgtaa gacaaattct tcgtcatcat caatacgata catctgacga
    14461 tcaattttcg gaaataaata aagacctgct tcaggtttaa ctgccgataa gccagggata
    14521 gcattaatag cttcataaat aaaattacgt tgctcaaaaa tacgccctcc tggcagtaac
    14581 agtgcatcaa ctgattgatg gcctcctaag gaagtttgaa caacttgttg ggctaaaaca
    14641 ttggagcata accgcatatt ggcaagcatg ttcaaccctt caatgtaacc tttaacatgg
    14701 tctttaggac ctgataagac catccatcca acacgaaaac cagcaatacg atgagatttg
    14761 gaaaggccat tcatagatac acaaaaaaca tcaggtgcca aacttgcaat agcaatatgc
    14821 tctttaccat ccatcaccaa acgatcatag atttcatcgg caaaaataat cagctggtgt
    14881 tctcttgcca aagcaacgat gtcttctaaa atctccttag gataaagggc tcctgttggg
    14941 ttattaggat tgatgaccac aatagctttg gtgcgactcg tgatttttga tttgatatca
    15001 gcaatatcag gataccaacc agcttcttca tcacaaagat aatggaccgc ttttcctcca
    15061 ccaagactca cacaagccgt ccataaagga taatccggca ttggtactaa aacttcatcg
    15121 ccattatcta gcaaagcttg aagggacatg gatatcaact ctgacacgcc atttcccaaa
    15181 taaatatctt caatgtcaac atctgggaag cccttgagtt ggcaatattg catgatagcc
    15241 ttacgcgctg aaaaaatccc tttactgtca gaatagccct cacttagcct cgcgttgaca
    15301 attaaatcac gaatcacttc atctggtgct tcaaaaccaa aggcagcagg attgccagta
    15361 tttaaacgca gaattttttc accactcgca atcatgcgat tagcttcatc caaaacaggc
    15421 cctcgaatat cataggcaac atgttctaat tttgatgatt tttcaataat tttcatagtc
    15481 tcatacacct cgtattccat attataaacc aaaatactta aaatagaaag aatttatcca
    15541 tcaaagctta ggacagctaa tcgttttaac acaggtctta tgagacgata atacagtctt
    15601 tagtttagcc aaaataatgg atcgctttca aaatgactca aaaaagagta taatgaaaaa
    15661 aagaataaaa gctattcttg tcaaactgtt aaaggaggac agttctatgt cactaaaata
    15721 taaacgtatc ctcgttgcta ttgatggctc ttatgagtct gaattagctt tcaataaagg
    15781 cgttaacgta gcactacgta acgacgctac cttactacta gtacacgtta ttgacacccg
    15841 tgccttgcaa agcgtagcta cttttgatac ttacatctat gaaaaattag aacaagaagc
    15901 taaggatgtt ttagatgatt ttgaaaaaca agcacagata gcaggaatta caaacaccaa
    15961 acaaattatt gaatttggca atcccaaaaa cttactagct cacgatattc cagatcgtga
    16021 gaacgctgat ttaattatgg taggagctac tggtcttaat acctttgaac gcctccttat
    16081 tggctcatct tctgagtaca ttatgcgaca cgctaagatt gacttacttg tagtccgaga
    16141 tagcactaaa acactctagt ctattacaat tttcaaacac caaaaaacaa gcagcatcta
    16201 caacgaaatt gcttgttttt ttgtgctgag ctaaggctag tcaatatctt tttatttctt
    16261 gatatttaaa agagtaaacc tctatttatc gaatcgttgt agtgattttt gtaattgttt
    16321 gtctaactct ttttttatgg ctacttcagg tgcgtgacgc tcttgccaat agtcaggttt
    16381 ttgaatttta tgggtaacgg ggtcaaagtg tgccttgcca tctggaaaaa ttttagccat
    16441 atttgcttca tgtactgctt caaatatagg ttgtggatct actcccatca gtacaaaaga
    16501 accataagta aaataaagca aatccgctaa ggcatcaact tgcccaatca ggggcgtttc
    16561 aacatgtttt ttagcttgta ccttattagc tgcttggtca acagcaccat gcaaatcgaa
    16621 aatagcctgt gtaaattgtt gttggtctcc cttgcttgct gcgtaaagaa actcaacaat
    16681 ttcttctact ttgaagcctg acctataacc agcttctgaa atggtataac tacgcggtgt
    16741 ttcaatagta tctgagtcca tcagacgatg aaaatctttg actttattaa aattttcatc
    16801 acggctttta aagctcttct ctacatcaaa atggatgaga ccgtagtgag ctaaggcttt
    16861 ggaaatccca tcattattat tagagtcggt agtgaaatgg gctccatctt ttaccacagt
    16921 ttcagcattt cccatagcaa caccgatacc aactcctgat aacatttcaa ggtcattatc
    16981 tgaatctcca aaagccatca cttcagacaa gtcaaatcca aacatctctc ccaaacgttc
    17041 aattcctttt atttttgatt gatcaacaga gatcagatcc aaggaataag gactacttct
    17101 tgtaattttt atatgaggga acttctcttg tattttctta gtttcagctt gtgacgctac
    17161 cagaacaacc tgataaatag gctcccgcat aatagttact aaatttctaa aactctgtgg
    17221 cttgattcgg cgaatcaaat gcttaaaact accctcaact gttcttgccc aacttttggg
    17281 cacaaaacta ctaatgactt gtccaaatgg gctagtcccc atatcaataa tacgcgatcc
    17341 tgctaatcca gaagctgttc caagagaaat ttctcgcttt ttttcgttgg catagcgaat
    17401 cactttgtaa atcatcgact ttggcaattg attctgatat aagaccttat ctcttgttaa
    17461 gatatattgg ccattgtaag taacggcaaa atctaatcca aaattttcta gaaaaggttg
    17521 cacaaatccc ggtccacgac cagttgctaa accgaccata atgccttgtt ttttgagttg
    17581 ttggatggct ttttgcgttg ttttttggat atttttacga tcattgagaa gggtcccatc
    17641 aatatcaaaa aagactgctt ttactgtcaa aaccttcttt tgcctcctgt gtttatgata
    17701 gaatagatta taccataaat aaaaggaagt ttgacaatca aatgaaaaaa atcctagtct
    17761 tacacactgg tggaaccatt tctatgcaag ctgataactc tggtagggtt gttccaaatc
    17821 aagataatcc tatgacgaaa atacatgctg cagcccaaga tattcagctt accgttagtg
    17881 attttctaaa tcttccgagt cctcacatca cgcctcacca catgttatct atttatcatc
    17941 atattcaaga aagaactgat gtctttgatg gtatagtcat cacacatgga acagatacct
    18001 tagaagaaac agcttatttt ttagatacca tggcattgcc gactaatata cctgttgtcc
    18061 taacaggtgc tatgaggtct tctaatgaag ttggtagtga tggcatatat aattacctta
    18121 ccgcacttag agttgcaagt agtgataaag ctaaagaaaa aggggtactt gttgttatga
    18181 acgatgaaat ccatgctgct aaatatgtta ctaaaacaca tacaaccaat atttcaactt
    18241 tccaaacacc cacacacgga ccacttggta tcatcatgaa aaatgaccta ctttttttca
    18301 aaactgctga acctcgtact cgttttgact tgagatgcat ttctggaacc atccccatta
    18361 tcaaagcata cgcaggtatg ggcgatggta gtatcttgag tctattaact ccaggtagca
    18421 ttcaaggtct agttatcgaa gctctaggag caggaaatgt tccaccatta gcagttgggg
    18481 agatcgagca cttaattact ttaggtattc ctgtcgttct tgtttcccgt tgtttcaatg
    18541 gtatggcaga acctgtctat gcttatgaag gtggtggtgc gatgctacaa gaagcaggtg
    18601 ttatgtttgt aaaagaattg aatgctccaa aagcccgtct aaagttatta attgctctca
    18661 acgccggact tactggtcaa gagctaaaag attatattga aggataatct agcttcctgt
    18721 agaaatgtcc cactaaaaca tattagtggg acatttctag tactttaagc tttaaattag
    18781 gacatctcct aaatgtctat aataaacaac tatgtttcaa actatgagat tgtgcactca
    18841 taatcatttc tataacagtt tgagaccagt attccaataa atccaactct tagcaatcag
    18901 ataccgggta aacaagtctt tattttgtct cattaagtat gagcttactt gaacaaaact
    18961 acttgctcat ccttttttag taatgatgaa gacgaccttt ttctaagaac ttctttgtct
    19021 tttaaaataa acaaagcaca tattaccctt tattttagtt ctcgtttgga aaaaagaaag
    19081 acattgccta aaagataaca aagagttaaa ccaaaggcca ctaatatgct agtactagaa
    19141 aggtgtttat caccaagaga caaggatagg tgtttaaaca tcaaaactgg gctaatatct
    19201 aacttaaaaa aatggtttgc taaagtagca gatactttat caaaaagaag gacataaagt
    19261 atataagctg tgattgactg ataagtttta cgaagtatat taaacaaaaa ttgacacaaa
    19321 gcaataatag caatataaaa aggcagttgt ttaatcatcc gatctatgac tattccatag
    19381 tatgcttgac cagtaaataa atgaaatgct aaagcactaa gaagataagt taaactaata
    19441 aataaaagag ataagccaaa actaactagc agtttataga ttataattaa agtacgattt
    19501 tgttgtttga ccacaacatt attaatcgta cggtagttat agtcatcact aaaaaagata
    19561 cgggctggtg ttaaaaagaa aagcgtcaca aaatcagtaa taatattaat gatttgtatc
    19621 atgatgactt ggttaggtgt cttgcgaaaa ctataggcta caccaacagc aatgacaaca
    19681 agtagcatac aagaaaatag agctaaacgc tcttggcgta tccgaaataa atctgcttta
    19741 agataggaaa tcataaaaac ctctttctaa attaatggtg acgctgcatc tttacctaac
    19801 aaatcgaggt aataatgttc aaagctcact tgatgatgat aaatgtcaaa aatctcaaca
    19861 ggcagatcta gaacaatttt gataagctcc attactgata aagttggttt tattagtagc
    19921 atctgaccat cagtctcaac tctaagcccc agttctaaaa gtctattttt taccagctga
    19981 tcatcgcttg tatgaagggc aatttgtact tttacttgag ctttaagctc tgctttgtcc
    20041 atactcttaa taactttgcc tttatgcata atcacgtaac gatcaacaac taagtctaat
    20101 tctgataaaa tatgactgga aatcaaaatc gtaataccat agcaatctct taggttcaaa
    20161 ataatctgac gcatttcttt gattcctgct ggatcaagac cattaatggg ttcatctaag
    20221 attaaaaatt ctggaaaatc tagtatcgaa agaccaatag ccaaacgttg gcgcatcccc
    20281 aaagaataat ctttcacctt tttctttttg ctcacttttt ctaacccaat aagttccaaa
    20341 acctcatgta tgcgctcttt tgcatcagct aatctcaatt gcaacgctgc atagtaaaga
    20401 ttatcatgag ctgtcatatt gggataaagt gtcggtgact caataattga tcccattcga
    20461 tagggttgta actgtttgat ttgccctgaa tcttttccaa ttaggccaga taaaatacgc
    20521 attaacgttg tctttccagc gccattttga cccacaaggc cgcaaatttc tcctttgtaa
    20581 atatcaaaag agatatcact taaaacctga tttttaccat aggatttatt tacttgttca
    20641 atacttagta acattgattg ctgtgtcatt ttccgctcct tttttgtact tatcgattaa
    20701 tacaatatct tacacataat aacatccgta aacggttgtg acaagttctt ctttttatta
    20761 aaaaaatata aataagaatt ttaataatat gattatttta gcaataaaaa gttatactga
    20821 aaatgggtta atgctgcttc aaatagcagt ctcatcgtaa agcataagta aaaaaggcca
    20881 gaaatagtga cctctttata ctttataaca gtttatctca aaactaacta aatatttgta
    20941 aaacaacgac ttgttttaag ttaatcataa acttcttttt ttctaatatt ttttgctact
    21001 aattgccact gtttttcata tatccagtta ggatcagaaa caatcgctgc agaaactttg
    21061 cgtgcttctt ctaaaatagg ataatcctca acgatatcag ctacttgaaa ttctggaatt
    21121 ccagactgac gagtaccaaa gatttcgcca gaaccacgca tttttaaatc cgactcagcc
    21181 aaaacgaaac catctgtcgt ttctgtcatg attgtcattc gttttttccc cgaatccgtt
    21241 ttgggattag ccactaaaac agcgtatgat tgtttatgtc cacgaccaac acgcccacga
    21301 agttgatgta actggcttaa tccaaaacga tcggcatcca taataatcat gatcgttgca
    21361 tttgggacat ttacccctac ttcaataact gttgtggata ctaaaatatg acttttttta
    21421 tccttgaaat cttgcattat ggcatctttt tcatcatttt tcatacgtcc atgtacaaga
    21481 gcaaccttag caattccttc aaaataagta gataattcag catgcaatgc tactgcattc
    21541 tttaaatcta aagcttctga ttcttcaatc aacggcgaaa tgacatacac ttgagcatct
    21601 ttttgcaatt caccttttac ccattccaac acagtcccta gctgctcgtg tttcacccag
    21661 cgtgtcataa taggtttacg accggcaggt aattcatcaa taacagaaac atccatttct
    21721 ccaaaagctg tgattgctag agttcgggga attggggtgg ctgtcatcat taaaacatca
    21781 ggattttctc ccttttcacg gaaaattcta cgctgtttaa caccaaaacg atgttgctcg
    21841 tctgtaatga caagccccag tttatggtac tgtaccgagt cttggataag agcatgagtt
    21901 cctacaatca tatcaaccga gccatttgca atagctgcta aaaccgtacg cttgacagcg
    21961 gctttcatac ccgaagttaa tatagcaatt gataaatctg gaaataactc ttgcagacta
    22021 atgtagtgtt gttcagctag gatttccgtt ggaaccatca aagccgattg aaaacctgct
    22081 gtgtaagctg catacattga tagaccagca atgactgtct ttcctgatcc tacatctcct
    22141 tgcaataaac gattcatatg agctcccgat gacatatcag ataatatgtc atctaaagag
    22201 cgcttttgag cattcgttag aataaaaggt aaagaggaaa tctttgtctc catagcatgt
    22261 ttactataaa gaataggcaa accatttgtt tcagatttat tttcggcttt caaaacttga
    22321 aggttcattt gaaagtaaaa taattcttca aatttaatcc gacggagcgc ttgcttatac
    22381 tctgtgatat cttttgggaa atgcatagct aaacaagcct gactacgacc catcaatcgg
    22441 tatttttcca ataaagtagc tggtaaattt tccttcaatt ccaaatgcgc atcgatttca
    22501 aaagctgact taatagcttt aatcaaagta gactgtgaaa ttccctgagc tacgcgataa
    22561 acaggttgca tgtcatcttc aacttgagct aaaaccttca tcccagtaat agccgattta
    22621 gtggcatccc atttaccaaa aacagctacc tcttgaccaa gttctatttt atcagctaaa
    22681 tagggttgat taaaaaaact aacatttaag acagcttccc cttgacgcaa tttgaaactt
    22741 aaacggttac gtttaaaacc ataatattgt acattagctg gagtaacgac taagcctgta
    22801 atgactgctt tttcaccatc cactaaatca aaaacagatt ttgatttaaa atcttcatag
    22861 cgaaacggat aataaagcag taaatcttct actgtataaa tatctaattt ctgaaatttt
    22921 tctgctgatt ttggtccaaa tccctttaag ttggacatag gagctgttaa tatcattatc
    22981 tttttccctt cacaaaaact cttgtcaaat tgaagacaaa gtttgtcgtg atactttcct
    23041 taggtgttgc ttacggcagc gacgttcttt ggaattccat gcctttattt ttgagcctga
    23101 ggtctcaaaa atcccgagtg gcgtcaatct cgtattgacg ccactctttt accacagcaa
    23161 aaagcatctg ttttggctca ctttatatgt aaaaatcgga aaccgaatgt caaatcagca
    23221 gagctatgca tgctttttga gcataaaact ggagttgtct acgttctgac tcttgtatct
    23281 attatacaag agttcctatt tattttcaga taaaagcctt ttagtacttt taaacagttc
    23341 taggggctcg ttaaggtagc atttgataaa tggtatcgca gacactactt agaagttctt
    23401 catcatgtga aatgaggatg acaaccttat gttgggtctt cagctgcatg agaagattag
    23461 ctaaagcctt catttgtaac aaatcaagtc cgctacttgg ctcatcaaaa attaagatat
    23521 ctttatcagc caaaagacta gcggcaatca tcactctttg ttgctcacct cctgacaaac
    23581 tagctggatg acgttctaaa agagtcgtta aagataaccg ctctattact tctgtcgtcc
    23641 gtggatgtcg ttcatggccc aagttgactt cctttgaaac cgattctgca aatagttgca
    23701 aagaaacttc ttgcaagaca atactagtct ttgataaacg ttgcttggca gataaaggta
    23761 tgccttgaaa cttaatagtt gcttttttat cgtctaagat accagctata taataaacta
    23821 attgcgattt tccaagcccg tttgatcctg taatgccaga aatacttcct gcagcaaaac
    23881 taagctgttc aatacatcta agtattttgc ttcccgctct aactgttaga tgctcaatac
    23941 acaggctatc atctgggcga taatgttgac tctcaatctt accagctaac acaggtttta
    24001 ggtcagacaa gtctaagcga cgtagtccca tatcttgccg ttgttcatct gtcaaggcta
    24061 gcaaatcttg tgttgttaac ttatctgtta atctaccatt tttaaagtag aaaaagttat
    24121 ctgccaaatc catcaagtaa tgtaaacgat gctcggctac aataatggtt tttcctgctg
    24181 cttttaattg ggtaagatag gctttaacag cagctatgcc tgcactatct aagttagcag
    24241 ttggctcatc aaaaagcata atattagtcc cttgcataat ggccgttgca atagcaactc
    24301 tttgtttttg ccctcctgac aatccaaaca tatccttgtt aaggagctct gcaaaagcaa
    24361 aatcttctgc taaagtccaa agacgcttca tgataacctt tgcatctaga ccttgatttt
    24421 cacagggaaa gaccagttcg tgttggactt cgcgatagaa aaactgactg gctggatttt
    24481 gaaaaacaga ggccacagat ctagaaaatg tttcaacact gtctctgcct gcttggcaat
    24541 cagccacatc tagtctacct tcatattttc cagcataata atcaggaatt atgccattaa
    24601 gaaattttaa aaaggtagat ttacctgaac cagaaggtcc acacaaaaca atgaattgtc
    24661 cagaggcaat ttgacaagtc gatacctgac aagctggatt tttttgatca tgataggtaa
    24721 aaacaagttg ttctgctgaa atcattgaag tatcgtgatg attatgataa cagtaatcca
    24781 aatctgcacc ccccaatctt tccatgttaa gtgtgagcta aagcattcac tggtgccttt
    24841 gttgactgct agtcctttag ttagactagc gatagttagt tcttgactac ttcttatcaa
    24901 agataaaagc aatggaacca ttaaatactc caagtattgt ttaggtctga tcaaaataga
    24961 ccattttgtt aaaataatcc ccctgattgt taaagagcga tgaataacac aacattcttg
    25021 tctaatcatt ggtatgaaac ggcacataac ggctaaggtc aatagccaga cttctgggaa
    25081 atgccatttg cgtaatccat gaaccaattc gtagatggta gttgtcttaa tagtaattaa
    25141 acctgctagt aaagaaggga aaactaacct tcctgctatt gagacaaaac tcaataagtg
    25201 atcaagataa gaagggaaaa tagatagcgg gattacagat agaccgatca ttaacagata
    25261 aacaaccgcc aacttgaacg ctagcttttt cttatttaaa gcagacaaaa gtaataagag
    25321 aaaaataaca attataaatt ctaaaaagcc atcaacacga aaaaagaagc aggcattggc
    25381 aagtaccaac aaaagcagtt tggtgcgaac atcaagcctc atgataacaa tcctgatttg
    25441 ataaaatgtt tctttagtaa ataatgtgca aaagctgacc ctaataaggc tcctgcaaga
    25501 acaattccta caaaccaaat aacatgccct aaactaaaat catacataat gcgattgaca
    25561 taggtcatac ttttaccgcg ggctaacaaa ctttcaacat aggcatcttt caacaaccac
    25621 atcagcaaaa tagggccact attgacaaaa gcaaaaacca tataagatag gtcattaaaa
    25681 aaccttgact tgtattttcc taaagacgca ataatatccg ctattaaacc gaaaataatg
    25741 ctgggaaaca tcgcggcaaa aaagtgacca ctaaggaaga aaaaacctcc catcatcaat
    25801 cctaataatg ttattggccc aaatgtttgc aatttagcaa tcaaaagaaa ataaattgtt
    25861 ccccccagta aagctgcaaa ggcaggggca tataacatat ttcctgagcg atcaaaaaag
    25921 atgttaataa acgtgcctag accaacacaa ataaagtaga gcgcggcaaa tgcgcctgtt
    25981 atcataatat cttttattgt taactgtttc attttttatt cacctctttt agggaaccat
    26041 tttttacgca aaatagatga tcagcacaag ctagggttga tggcctgtga gaaattaaga
    26101 agaccaatcc ttgacaatgt gttttaatta aatcaagcaa gatagcttca tttagagaat
    26161 ctaaatttga cgtgggttca tcaaaaatat agcaactcgc atctttcaat aaagctcgca
    26221 tcaactctaa acgttgacct tctccagctg aaaagtcaga aggactcttg ataatcgtat
    26281 ccaaaccttc cttacaagct aacagtctgt ctttcatacc acaagcctca gctaaattcc
    26341 aaatggactc atctgaaata tcttgacggc caaaggtaag attttcacgg atagactgtt
    26401 ggaaaagctg cgctgtttgt ggcacataag caatagtatc ttgtaattta gcagcattta
    26461 gtaagcaact attcctatca ttaagaaaaa tatctcctgt cttccaatta taccatttca
    26521 taattaattt caccaaagtt gatttaccag atcctgattc gcctttaatg ccaataatcc
    26581 ccttttcttg aaaggtaact gataaatcct taaaaaccag ctcttggcgc tttggataag
    26641 caaaactgag gtcctcaaaa gaaacactcc ttaaacgatc tatctgtttt gtgccttcgt
    26701 ctactatgac attttcgtct agaaggtcaa aaatatttcg agccgcattc atagcacgct
    26761 taaagcctaa tggcaaacgt cctagttcta aaaaaggaga aaacgaagca gtgaaagcaa
    26821 taaaagttaa taagccttta tcaaatgata agctgtcatg gcatatccca tcaaaaacca
    26881 tataagaaaa tgctaagatc attaagccaa gccagaaaaa agtcaatgcc atttgcaaaa
    26941 attgagcttg cgctacgtta cgatcaagag catttacatg attacttttt tttattaatc
    27001 gttcaaattg ctcgtctaat acctgaaatt gcaaaagatc tttgactgat cgtaaactct
    27061 ctaaaaaata agacaggtaa tcttttcgac cttcattttg gctttttagt aaaacctgta
    27121 aaatattagc aaaataaatg ggtataacaa tggctaaaca agcataggtt gccaaagcta
    27181 ataaggctaa ctgccaacta ctttgacaaa agtaccagaa cattaaacct gcagacaata
    27241 ttgctgtaca aataggtgcg attgtgtggg caaaaaaaac ttctaatgcc tcaatatctt
    27301 ctccaatcat tttaagcaaa taaccactgt cttgactatc caagcaagct ggagaaagag
    27361 ctcttagttt cttaaagatc aagttcctaa aagctgctaa actatgaaaa gcaacaaaat
    27421 gaccaaaata atgctctcca taacgaacaa aacctcttaa aaaggctaga gcaatcaaaa
    27481 gatataaagc taatattgga atcacttctt gtctaatagc tagtagtcct aaataagcta
    27541 ttccagttgg aatacttacc gtgatgacaa aacccataac agcaaagcca acagccaaga
    27601 taatccaagg tagcagagtt ttcattaggg ttaataaacg ccaaaccagg cttaaggtag
    27661 atctgttagg tttagccata aatacttgcc tccagtcttg cttgagtatc aactaggtga
    27721 cgataagttg gttggtcctg ataaaggtcg caaggtttgc ctaaacaagc aggctgccct
    27781 gtgcttaaaa agagtacttg gttagctcct ttcagtcgag acatcttatg agtaatcaca
    27841 ataactattg ccgtcttagc taatctcgtt attaaattgt caataatacg ttcattttct
    27901 gcatctagac tagatgttgc ctcatcaaag atataaagag acctcttact taatagagct
    27961 cttgcgcaaa tcacctgttg cttttggcct ggtgataaca ggttaccatt ttcaccaact
    28021 attgtgtcaa gaccatctgg tagccagttg atgaagctta acaaaccatg ttgatctatc
    28081 caatctaaga tttctttttt agagagattt gctgctaacc ttaaattatc gtaaatgcta
    28141 cggttcaata aagtagattg gtccgataca taaagaacct gctggttgat aatttcttgc
    28201 gacaaattat cactgtctaa accgtcaaat aaaatatgcc cagtcgttgc agactggcgt
    28261 ttcaagagga gttgagctag acttgttttt ccttgtcctg acacccccgc gatagctgtt
    28321 agttggccct tcgttaatgt catggtaacc ccactaagca ctgttttttt ctcataagct
    28381 aaagaaatat tttggatgtc aatctggtta aagtttattg ctgttttact cttgttatct
    28441 ttccttgctg gaacactatc caagaaacta aagatacggt cagccatttt ggtattcatc
    28501 atgaccaagt gcatgccata accctgctca cgaatcggtg taaaaaattc tgtagcaatc
    28561 aagagaaaaa agaggaaatt aaaaaaagat aactggcctg ttgccaaaga ttgaacggct
    28621 aaaaaacctg acaaaccaat tcccagatac ataactgcat ccatataacc aactgcttgt
    28681 aactgaaagc caagcaaaga catggtagcc tttcgaaact gttctgcctt acccgaaaat
    28741 tcttgctcgt aacgttctgt tgcttgataa gaatacagtg tattaagacc ttttaagtca
    28801 tccataaata aattaccaac atccatataa gaagaccaat aatgactcat aatttgctta
    28861 gaccgctttt gcatagcaac gattgaaagt ggaatcagag gcagaactcc taaaaagaca
    28921 aggcctgcca atggataaat taaaaagact agtatcataa tagttgtgca gttccacttg
    28981 gttctcatag acaaagacaa atagtaacca taataagtat ccaaactatc aatcccctgt
    29041 gaagccaacg ttaaaatatc agcatcactg gcatgagcat caaattgccc gtctaaatca
    29101 ataaaagcct caaagaaaga ttgtttgagt gaatctctag caaattgtga agcgatccct
    29161 tgcagttgct tgcctaatat agcaagaaca aagcctacca catttaaaag taggagtaaa
    29221 cctgccaagc gtcctaaggc aatagcatga cccaaaataa aggtcgaaaa cgttttggca
    29281 atgaggtaaa aactgatcat cctcatcaca aattgtaacc aagataaaaa ggctgacaca
    29341 taaagcaaat acctttttgg tgcaattcgt tcacgtaatc gttttaacag tcttttcctt
    29401 tttccccttg atattgtatc ttctgtttcc atcatctgtt cttgttccta tctctccttt
    29461 gagttctaat atcattggtt tcattgacca atgaagtgag gacaaactgt tgaccatttc
    29521 tctcaacaaa gggcaattca acaccaaaga catctctgat aaactgttga tcaaggaggt
    29581 ctcttggttg cccttgggca taaagtcgcc catctttcag gccaataaga tggtctgaaa
    29641 gagacaaggc ctgattgatg tcatgtaata ctaacacaat cgttaggcct agctcttggt
    29701 tgattttagt aatcagcttg agaatgtcaa gctggtactt aatatccaaa taagtcgttg
    29761 gctcatctaa aaaaataata tctgtatcct gggcaagggc catggcaatc caaaccctct
    29821 gctgttcacc accagaaagg tgttggatgt cacgataaac ataatcactc aaagccgtta
    29881 cttctaaggc ccaagcaagg cgttcctcat cttctttggt agtagtttta aaaaaagaac
    29941 gacgggcaag cctagccatg gctaccaatt cacccactaa caaaccttcg accacttgat
    30001 ggtattggtg taccactgcc acttttttcg caaaagcctt gagagaaatc gttgctaatt
    30061 cttcctgctc aagccaaacc tgcccttgtt ttaacggaag gtttttggtt aataattgta
    30121 ataaggttga cttgccactg ccatttgccc ccataatagt ggtaattttc ccttttggaa
    30181 tgctaaaggt caggtcatct aaaacaagtt tttcctcata agcaaaggaa aggttttgca
    30241 cctgaatatg actgctgttc ttactcatat aatatcagac ctccttagca ggtaaataaa
    30301 gtaaggcccg cctacaatac tcattataat cgcagggcta atttccaatg ggtaagcaag
    30361 acttcttcct aaagtatcgg ctagcaaaaa gacaaaggct ccaagcaaag ctgaaaaagg
    30421 aattaagatt tggtgcttac ttccaaccag caagcggctc atatgaggga caatcaagcc
    30481 taggaaagaa accactccaa cgatcgaagt ggctaccgaa gccaataaaa cagcaaccaa
    30541 ggaaattccc aaccgtaacg ctgtagcatc gattcctaaa tgacgaatca cctgatcatc
    30601 taaaagaaga aggttacaag tttttgacaa taacaaagct agtaacaagc caatgaatgt
    30661 ataagggaat aaaaccccta catcagccca tgttttttga ctaatatgac ctgctaaaag
    30721 agcattgacc atgggactag ctgaggtgaa aaaactggtt aaagcagttg ataaccccat
    30781 aaataaagca ttaatagcaa tccctgttag aataagccga ataggattaa gccctttctt
    30841 ccatgctaaa ccataaatca gcaggaaaga cactccgcct cctaaaaagg atacgattgg
    30901 accatacagc agcaactgcg gtagaagtaa actagagact aaaaccataa aactagcacc
    30961 actacaaatc cctataatag cagggtctga aataggattt ttcaacactg cttgaaaaag
    31021 aactcccgaa acagctatcc ctgctcctgc taataaagca atgacaattc ttggaaagcg
    31081 caaatcataa atcaaagcca cttgggggtc ataagccaca aacagtccct tgacaattgc
    31141 ctcataagaa accgccaaac caccaagaga taaggcaata accgccaaaa acaggaggct
    31201 gagaattaaa agaactagat aaagaccact tctgaaacgt tttttagcca gtagggattg
    31261 atccctttgt aaatctgatg ctgtcatgtt acggatgatc tcccatgcgg tcaaaaagct
    31321 gtgttaaggt gtccaaggct tctgggtagt tcaatttagc actcatgcca aacagggtat
    31381 tgtccaaatc atagactttc ccttccttga ctgccgtaaa atgtttccaa atatcatttt
    31441 cagcaaattc tttgtcaaac atcactttta ccttgtctgg aatggcgtga gctgttcgta
    31501 aaatcaaatc aggttcctta gccagcatgt cttcaggatt agctgataga aattctttct
    31561 catctgactg ataaacattc tcaccacctg ccaaatccaa aagattccct acataagatt
    31621 ggttcgtcgc caccaaataa ctacctggca agcccataag aataagcact ttaggctttt
    31681 tcttcccctt acgtttagct tggaaagcac gataatagtc ctggtattgc tggcgcaatt
    31741 cttttgcttc ttgttgacgt tggaaaaggt tccctaaatc atcaatggac tggtacatgc
    31801 cctcaacact tcgtaagttc aaaaaaccat actcagtatc caatttttga tacttgggtt
    31861 ccaaatcttc ttgtaaagaa ttgggactca aaatccaagt tggtttcaaa gaagcaatca
    31921 actctatatc aggattcatg ggtaaaccca cacgcttaac agcatcatag cgtttaggaa
    31981 gggtatataa tttactatca caaaccccaa cgaggtctaa atttaaacgg tcacagatat
    32041 caaccacagc aaccgaagtg gctacaattc tctgctgttc agtctcttta gccgttttag
    32101 ggtgctgatt cacacaagct accaaaaaga aggccagtaa ggctagacca attcctttac
    32161 atcgttttat catttttttt cctctttttc caaacccata cagcaaaccc tcctaacatc
    32221 agaaaaaaag ttggaaagta gtaaatcatg attgataagt cttttttaga ccgaaactta
    32281 tcttcttttt ttaactctaa agaagtcgtt aagcccatct tagaaggaag aattttttta
    32341 tctgatagac tcttgttatt tgatgagttg gctttaggct tattctgagt tatcatagca
    32401 cctgtgtgag actcatcttg actttctcct agttttgtat caacaggctt ttgattgtct
    32461 tttacttctt gattttgaga attgtctgtt tcagtaacaa gctgggccaa catattacct
    32521 gaatactttt gtattaactc tgaagctgac aggtaaaaga ccacttctct gcccataggt
    32581 tcaacaaaca tgcttcctcg aatgatacta tttacagtag gcaaactaat agcaatatct
    32641 agtgttgtcc cattggtatc agtacctttt tgggtgatat tgtaatccac tgcctgaaag
    32701 cttccagttc ctccaggttg tatccagaac tgataattgc ccgaataatc agctaagctc
    32761 atacgaaaag taagaacgat ctttccagca tcagatactt ctagcatggc atcactatag
    32821 acagtccctt caaccatacc ttgaccaata tcaaaggaat gctcccctcc gctatcttct
    32881 atctgcccag atatcggatg acgataattt ctttgaataa tacatccata aatttgacct
    32941 ttatctgcat acacaacacc tgtcattgtt gataaactta tcatgaacac taatattacc
    33001 tgcaacaatt gctttattac tacctttgtc atatgctcct cttgtctatc ttttcttaaa
    33061 catcttattt cattttttga atgaatccca ttttataaca tctccaaatg tgaaaaacac
    33121 acctatcttg cgactacttc aacctgtatc aggagtcctg tcggaaatag gtatgttttt
    33181 agctcttgct gactagcttt tttgttattt cacttggtac taagtttctt atttaaataa
    33241 tgtctttcga ccaataatga atcccagagc tacgatcata aagctagata gacctgcaat
    33301 taagtgataa cgagagctac tttcttcacc atttgattgt tgattggaac gatgtttctt
    33361 atcagatttt ttagtcttag tatcttttgc cgttttacct tcaattgatg tttgatggtc
    33421 acgcactaac tgcttcaatt tctctgcctt atcagtatca tctgtcttag aagtttcttg
    33481 agttgattgg ccggttgctt ttttagccag gagatcagtt aagttagcaa taccagtagc
    33541 tgctgaagga ggacttgtag gtgttttttc ttgttgacga ttttgaggtc ctttcaactg
    33601 atctgttgaa gttagatggc tattttcttg tggattatta gtttctttga caacctttgc
    33661 cttctctgtt gtaaggcttg cccagtctaa acgcataaaa acggtttgaa ggccagaccc
    33721 ttttgaaatg gcatccatga taggcacaaa gacctgtaat ggcacaagtc cgtctttctt
    33781 cgcttgttca atcaatttca tacgcagcac ttttggatag ttttttccat aacgatctgc
    33841 caatggtagg ccagttacat ccgtgaaata agacataacc tctgcctttt ttgttcgacc
    33901 aactggttta ccagctaaat ctctttggta accatctacg aaatagctca attcaccaag
    33961 gtagcctaac attttaccaa cctttatacc tctaaactca acttctaaat aggaaacacc
    34021 ttttttaact accaaagtca ctcgatgatt aatagccttg tttgacatgg actcagatgc
    34081 taagtcagtt tttaacatgc tggcatttaa atagtaaata ccatcacgca attgtttttg
    34141 atttaatgct ctaaggcctt tctcaactaa atctttctga tgtgaatgtg gtcctttcgc
    34201 cacaaggata tcattagatg tcaaatagat atgttctttt tgagtagcaa atttctttgt
    34261 tattaaagct ttgcgaatcc gatatgtacc tggatctttt aaagaaaact ctcccgttaa
    34321 gtcatctttc ggatcatttg agagaagctt ttcgtcaacc gtcacatact gtccattgga
    34381 agattttttt tgcaactgaa tcacaatact ccaatcgact tgcatatcat taagtaaaga
    34441 gacagtatct gaaccttttt cttgtaacaa ttgttgatat tcttcaatag acactagttc
    34501 tttatcaatg gcagaatttg ttttttgctc ccaataatac aaatctaaaa gactgaaagc
    34561 ttgatgatat tttatttttt cgccatctaa ggaagcttca agttttgcaa tatgtttttg
    34621 tggagtcagt ttgtgttgtc cgataagagc tcttactttt ggactcacac tttttatctg
    34681 attgaacttc acatctaaat taagcgtttc aacatctgga aatactgatt cttcaacctg
    34741 agtaagcaag ttattgaaaa aatcaagttt tgttaattgg ttattctgtt taaatatacc
    34801 atttggaaga tttgaaattt cattagttga taaatcgagt acagataatt taggaagatt
    34861 tttaaatgaa atattatcaa tacgagtaat atgatttcta cttgccacaa tagtgcttaa
    34921 ggcgcgcaat ttttctattg tttttggcag tagtgctaaa ttattttgag ataagtcgat
    34981 aaagttaagt gatgttagag gctctagcgc tttttcaggt atctcctcaa gactattttc
    35041 tacgaaacct aatgaagtca ggcgagacaa tccttcaaat gggttatcac aaagtcggcc
    35101 aatacgattc ttggaaagat caagttgctc caggtgatga agcgactgga atagtcctcc
    35161 ttcaagagag tgaataaagt tacttgataa gtagagttct cttaattgag cattgctttt
    35221 aaatgtatct ttgtctagat aagttaaatg attacttctt aagttaagaa atcgaagttg
    35281 cttcatttga ctaaataact cttttggaat ttccgtaatt tggtttccat ccagatgtag
    35341 ttctgtaata tttttagcat gtttcaataa agaaatatca gttaactcgt aatggtctag
    35401 acgcaactcg cctcgaatct gttctaattc cgtttttgat aatttaccat cgccgtctaa
    35461 atctttgcca agtttaacta aaactttttc aaggcgttca gtcccaaccc ctttttcttt
    35521 atcatcagtt tctacctgat aaccactcca gcctttggta acggtcttac taaaggtcat
    35581 gtcaagtttg gtgtatttgt catagtggtt taaatcactt tcttgacctc ccatggctga
    35641 taccaaaaca gcacctttgt gtaatttatc caaatcatca ataggcatgg taaattcgct
    35701 tcgaatatag ctaccattga tatctttttg gccaacttgt ttacgcactg ctgctgggta
    35761 ggtccctttg ctttcaatag aaaagtcaat caaaaattgt cccaaagcag tattaagcat
    35821 ggaaatttcc attgtaccat ctgctttaac cactaatttg gctcttttat caaaagcacc
    35881 ttgaagcatg gaggactctt ctgagttttc tttattagct ttaaagttta gcgtataggt
    35941 tccttctgtg agattgttaa tagaaacctc acctgcttga taagactcga tggtatcttt
    36001 taacagtcga tgagtatcgg cgagtagctt actatctttg atttctttgt aagaatcctt
    36061 gatgacttga agtttctcag tcagatcttt gaccgcgtct ttgttgcttt ctttagcaat
    36121 aatcgcatct gcttctttga tagctttttc caaagtggtg acatccaact ttgttgattg
    36181 tctgtcttca gcagtcacat gttgtttttg tttggcagat acaaaatgga tttgaccatc
    36241 tttcttggta acggtaatgg taacgtcttc aaatcctttt gaggatatca cgattgtatt
    36301 ttctccatct ttgaaagctt ttgttccaag atgaagccct gtcatattcg agacccagcc
    36361 attagtatct tttacagaat ctgaaataag atctttataa actgtaccat tgactgtaac
    36421 ctttgtgatt tgctgagtca aagcttttat ctgtttgtta tcagaataac gatcaatttc
    36481 tggaaataaa attgcaccat cttcttgact caattttgtt attaatgaca agttagcagt
    36541 atttttccca gatttaacaa ctttttgtgt gccttggttt tgtggcactg gtttatcact
    36601 gagcacatct ttttttaatg aatcctgatg atctgttgtt cctaattttt gagctgaaat
    36661 aaacgtcact ttatcgccct ttttaataaa ggtaattagg atatcaccat cttttttatt
    36721 aacaatagtc aattcatgct taccgtcttt aataaggtta gcattgatct taatcccttg
    36781 gtagttaata tcaaaggttc gctcgccttc caggcttttt tcatagacat cattatcaac
    36841 aataaccttt tcaatatgtt tataatactc ttttccagct gattgactag cgtaagggcc
    36901 tatctcttca actaaaagtc gattatcctg agttaaagct actgttgtca cgagttgtga
    36961 ctgaactaaa gcctcttgtg attttacagt ttgagtagcc tgaatgagaa caagggcaga
    37021 tattgccaca aacgcacatt tgctgatttt tttcattttc aatcaacttt ctaaaaattt
    37081 ttatgaataa gctaactttt ttattaactg aacttaatct atcactttgt gattattttg
    37141 tcaagattgt ttccaatggt ccaattgtct gttaaaaagc tccactatgt gagctaagac
    37201 actagtaata agcctggatt gataatgagg atatctctga taaaagaatt cctctacaac
    37261 aatattactg agataaagct agccttagga tagttattac tagggctgat tcaatcatta
    37321 cttcattcac aaaacatgta acacgttata agctccataa ttgtgttagg agacaacatg
    37381 ggatgtcact actgcatatt catcacaata aaaaaagaca atagccctaa tcgtgctatt
    37441 gtctcaaaat catttattta cttgaaactt tatcgtgtta caccaacagt ttaaaaggcc
    37501 taactcccgc taagaaaatt aagccgtaag aaaagtcatt agttattagt ttttactctt
    37561 gccaataaca tatgttagct gttcagcctg ctgagctgta aaggtacgat aagaaatctt
    37621 acctaagccg atacagttag actctgaaat aagaatagac ccatcttttc taacatcttc
    37681 tacaattgat acgtggccat aagtgccatc agcgcccgct tgccctggtg caaatgaaat
    37741 agcataacct acttttggag tctttgaaag ggcataccct gctttatact tccaatctcc
    37801 accatttccc ataaaaggat caaatcggta acccaattct ttggcgcgat tatacacata
    37861 ccaagtgcat tgtccccaag cataactcga agcttgataa tccaagacat cataactctt
    37921 agagagctta taacctgctg gcacagatgc aaaactagct gatgactgca ctttcaattc
    37981 ggctggaatg gctggtgtgc caccatggtc atcaatccac ttatccaggt cttccataat
    38041 ttgtgcgcga cgtttaccac taccagtagt atcagtaaaa taaacacccc cgtcacttgc
    38101 aaagtttaat tgacctcgtg aaaacttagc cgcttttaaa tcttgaagtg caaaattgct
    38161 atttttgttt ttaataatgg tatcttgtgt cattttgaca atagcactat catcattaaa
    38221 cttacttgct tgagtcggat ctagatcaaa agctgcatag ccaaacatat tagctccaag
    38281 caaagtcgca atcccttgag ttcctaaaga actctcagac atcgcaatag ctacgatagc
    38341 tcgaacatcc agcccacttt tcttttccca ttgcaatagc ttctcaccat taatacgtgt
    38401 gctgtcatag gcaatgcctg ttgattttaa gtaactatca atctggtcag ctttaatacc
    38461 ataacggcga gataaaagat ggtgggtata ggcattttta ccactccaat gctctacata
    38521 tgccgcgtat gtgttgacag aagggatatc tatctcactc aaatctaaat ctgatacgta
    38581 aggtgacaac gactgaatag gagttcttac ctctggcaac tgtctcgaca atgaagaagc
    38641 aggtgccgta tcaactttag atggtgatgg tttgggctga tcagtcggtt ttggctggtc
    38701 agttggtttc gaatgatcag ttggtttcga atgatcagtt ggtttcgaat ggtcagttga
    38761 tttatcatct acctggtttt ctgcttctgc cttgttgtcc gaagaaccta cagcagactg
    38821 actagaatcg cttctttttt tatcatccgc gttaacaaga ctattagagt ttgtttctgc
    38881 agaagcatca aggggacctt ggtcagtttt atctgatgtt gtaggtgctg catttttatt
    38941 attttcctct tttgaagaag aatcactggt ttcatttgta gttgctttat gcgtctcaag
    39001 cattacatct tgagccaaca ctgtcatgct ttctgctgtc ggggctaaga gacttagtag
    39061 catggcaact cttaatagtt tgtttttatt
//
LOCUS       DAUCWU010000009        33964 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUCWU010000009 DAUCWU010000000
VERSION     DAUCWU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33964
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.009"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..338
                     /locus_tag="VGY24_000452"
     CDS             <1..338
                     /locus_tag="VGY24_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ4283461.1"
                     /translation="TGLVEAGDFYYLNYCVGNVGQDIESQINGAFDEMERRLALVGLT
                     LDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFAHHGGPQGLLFQVDGV
                     AYSKHISMT"
     gene            377..2713
                     /locus_tag="VGY24_000453"
     CDS             377..2713
                     /locus_tag="VGY24_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4283462.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQVPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            2762..2914
                     /gene="rpmG"
                     /locus_tag="VGY24_000454"
     CDS             2762..2914
                     /gene="rpmG"
                     /locus_tag="VGY24_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4283463.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            2928..3104
                     /gene="secE"
                     /locus_tag="VGY24_000455"
     CDS             2928..3104
                     /gene="secE"
                     /locus_tag="VGY24_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ4283464.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(3340..4113)
                     /locus_tag="VGY24_000456"
     CDS             complement(3340..4113)
                     /locus_tag="VGY24_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ4283465.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            4239..4985
                     /locus_tag="VGY24_000457"
     CDS             4239..4985
                     /locus_tag="VGY24_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019317626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4283466.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQNKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            5001..5981
                     /locus_tag="VGY24_000458"
     CDS             5001..5981
                     /locus_tag="VGY24_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ4283467.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTVISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFINAMVIQEDKQLTKGIIQSKYFESILASWDQLDLALVGIGGEPNSRE
                     ESQWRDLLTDADYAQLKQEEAVGEVCCRFFDQAGHPVYKGLQDRTIGISLEQLGRVRK
                     TIAVATGKYKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            6262..6582
                     /locus_tag="VGY24_000459"
     CDS             6262..6582
                     /locus_tag="VGY24_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4283468.1"
                     /translation="MELIVPDQIIMGLILNAGDAKQHIYQALKCAKAEDYDTSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFIHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            6610..6918
                     /locus_tag="VGY24_000460"
     CDS             6610..6918
                     /locus_tag="VGY24_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4283469.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIDAYSQGKLAE
                     YAPLIDVALLGPQVAYTLDKSEAICKENGIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            6928..8232
                     /locus_tag="VGY24_000461"
     CDS             6928..8232
                     /locus_tag="VGY24_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4283470.1"
                     /translation="MAKMNMRNIIMPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            8443..10860
                     /locus_tag="VGY24_000462"
     CDS             8443..10860
                     /locus_tag="VGY24_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ4283471.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            10873..11541
                     /locus_tag="VGY24_000463"
     CDS             10873..11541
                     /locus_tag="VGY24_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ4283472.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGEIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIEQLAEAINRENA
                     NSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            11598..12686
                     /locus_tag="VGY24_000464"
     CDS             11598..12686
                     /locus_tag="VGY24_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ4283473.1"
                     /translation="MKVFASPSRYIQGKNALFTNVKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(12768..13277)
                     /locus_tag="VGY24_000465"
     CDS             complement(12768..13277)
                     /locus_tag="VGY24_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ4283474.1"
                     /translation="MSTDHQLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            13641..14456
                     /gene="sdaB"
                     /locus_tag="VGY24_000466"
     CDS             13641..14456
                     /gene="sdaB"
                     /locus_tag="VGY24_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ4283475.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(14697..15539)
                     /locus_tag="VGY24_000467"
     CDS             complement(14697..15539)
                     /locus_tag="VGY24_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4283476.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            16480..17676
                     /gene="speB"
                     /locus_tag="VGY24_000468"
     CDS             16480..17676
                     /gene="speB"
                     /locus_tag="VGY24_000468"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ4283477.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSSSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDVNGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            17678..17983
                     /locus_tag="VGY24_000469"
     CDS             17678..17983
                     /locus_tag="VGY24_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ4283478.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     SGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQKQISSLD"
     gene            18463..19392
                     /gene="prsA"
                     /locus_tag="VGY24_000470"
     CDS             18463..19392
                     /gene="prsA"
                     /locus_tag="VGY24_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ4283479.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVGD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTTAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(19669..19905)
                     /locus_tag="VGY24_000471"
     CDS             complement(19669..19905)
                     /locus_tag="VGY24_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283480.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPTAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            20329..23268
                     /locus_tag="VGY24_000472"
     CDS             20329..23268
                     /locus_tag="VGY24_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4283481.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSTTGSTETSAAS
                     TASVDATTSGTTASGASGESSDASVASSEGSQGSKSAPASPQPQPQAQTAPAATSASS
                     KAKTEEQTPKAATSSTPSTPAASSSSNSNQEASAETEPQMMDVEKYTVDKESSELKVK
                     DGKKPKNENKVDKDTKLIRNRDGEQRDIFDIKREVKTNADGTIDVTVTVTPKEIDKGA
                     DVMALLDVSQKMTKENFDKAKEQIKKMVTTLTGEPTDGKENRNRRNSVRLMTFYRKIS
                     EPIDLSGKTSEEVEKELDNIWDKVKKEDWDWGVDLQGAIHKARDIFKKEKESKKRQHI
                     VLFSQGESTFSYDINDKDKNNTVRKNRITGKVTTSNPLFPWLPIFNHTNQKAEVIDDV
                     DKLLDFAEKMGISLPKGLRAGVQAIGLSNSFLSTFTGSGLTEYLTLNEYGSDILKEKQ
                     FDYTKRVGEGYYYHSYSKRTHGDKMPFEKQIREVLEQFLPKIESREWAKKFIDIFGLQ
                     GQKVDQIGVDVIMKVINSIFYSRQYYYYNHNLSAIAEAKMAQEEGITFYSVDVTDLKP
                     KRAKRQAAVLQKTTKKESEEDRNNKFDGYLKKMSEGGKEFFNDVDEADKFKDTLTEVT
                     VTETFENSVSVENQSWKVSSNSNHKENVKYTNAQKNRNWFSSEVKESLTWTISKEQLK
                     QAFEDGKPLTLTYKLKVDNNKLKKALDDKRKNRPKRSIPTENENSVTEKIISNTVNYK
                     INKQEVKGNKLDDVKLTYTKETVPVPDVEGEVVPIPEKPLVEPMTPLYPAIPNYPTPQ
                     LPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDPIVDITEDTQPGMSG
                     SNDATVVEEDTAPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHF
                     DNEPQAPEKPKEQPSLSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKK
                     RRDTEEN"
     gene            23448..25385
                     /locus_tag="VGY24_000473"
     CDS             23448..25385
                     /locus_tag="VGY24_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4283482.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAIANNSKYQAQYELVKKE
                     LDALKATSPKLGERSLYFRLVNKFLSWFGQVWDPLAIKETDYLLDLIGQVNYLVSNTE
                     FQAPEYKDAKNKLDLAVAGYELYLAREKERGDAPDYQVTQLTLDQLETIKTKIQKALE
                     TFAQEVKTIQQTHPDLFPHAYVLNAKSKDITDKAQPFLVNGYYDVFYKQFLFTNTNDG
                     YNYNKEADNLASYQNLIKELLTLKAEIDQLTKGKTGDDRLETLMWESKFTLSDFRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVDIKEPEKAKKYHVED
                     ETSPSQPEAPAKPEAPSPGPAPGQKPAEDEGSSQAMEPANPSKEDSSTDASQGSHDSE
                     NPATDSPSQPQAPDQGNHQSQVPNAKPQTDKTDTPNVPAPPQDTPKVPEAGGQSGPAG
                     NAEEKAPDSGPKESGQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITDAPPT
                     VSGHNASTQPQSVVEDTASQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINEDTKP
                     KRVFHFDNKEPQASEKAAEQKLALAPHDSHTTPQASDDTAAPHLPASGDKPEKVFALA
                     ALTMVAAAGLLSKKRRNTDKN"
     gene            25527..25823
                     /locus_tag="VGY24_000474"
     CDS             25527..25823
                     /locus_tag="VGY24_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ4283483.1"
                     /translation="MTKQDQLIVEKVEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            25971..26255
                     /locus_tag="VGY24_000475"
     CDS             25971..26255
                     /locus_tag="VGY24_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ4283484.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            26679..27947
                     /locus_tag="VGY24_000476"
     CDS             26679..27947
                     /locus_tag="VGY24_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ4283485.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKIYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            27934..28608
                     /locus_tag="VGY24_000477"
     CDS             27934..28608
                     /locus_tag="VGY24_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283486.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            28621..29838
                     /locus_tag="VGY24_000478"
     CDS             28621..29838
                     /locus_tag="VGY24_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283487.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            29932..30585
                     /locus_tag="VGY24_000479"
     CDS             29932..30585
                     /locus_tag="VGY24_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4283488.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            30582..31970
                     /locus_tag="VGY24_000480"
     CDS             30582..31970
                     /locus_tag="VGY24_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4283489.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHYDD"
     gene            32072..33700
                     /locus_tag="VGY24_000481"
     CDS             32072..33700
                     /locus_tag="VGY24_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4283490.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDMDDESETAKKDKKSKETASQHDTQKDHKPSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNNTNQSDSSDQSTPSPKDQSSQKESQNKDGRSTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKAAEKTPEPNRDAPKPIQPPLAAAAPVFAPWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGEVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
BASE COUNT        11365 a         6869 c         6893 g         8837 t
ORIGIN      
        1 atacaggcct tgtagaagca ggagacttct attatttaaa ctattgtgtc ggcaatgtcg
       61 gacaagacat tgaaagtcag attaatggag cctttgacga gatggagcgc cgattagcat
      121 tggtggggct cacattagat gcagttgttc aaatggattg tctatttcga gacgtttgga
      181 acatacctgt gatggaaaaa atgattaaag agcgctttaa tggcagatat cctgctcgaa
      241 aatcgattca aacggaattt gcacatcacg gtggaccaca aggactactc tttcaagtgg
      301 atggtgtggc ttattcaaaa cacatttcga tgacctaatc aaggttactt taagatcata
      361 gtggtataat aacgttatga gatttctaga acttttacaa aagaaatttt ttcctaaagc
      421 atatcaggaa aaacaattct taatgcatca aaaaacgcgt ttaacgccac aacacaatca
      481 aaagcagtat tcgccaaatg ccaatcattt ggactcatca gctaccaaaa actcagaaca
      541 agtccctgca atagctctgc aacgcagtag agcctatgaa ggaagcccta aaagtcggcc
      601 cgcttggttg caaaagctgg aagctgtttt gccgtctcct caacatccaa ttcggcgttt
      661 ttggcgccgc tatcacatcg gaaaactgct aatgattctg attggaactc ttgtcttact
      721 cttaggatca tacttgtttt acttatcaaa aacagctaaa gtatctgatt tacaagatgc
      781 cttgaaggct acaactgtta tttatgatca caaaggagag tatgcaggca gtttatctgg
      841 tcaaaaaggg agttatgttg agctcaacgc tatttcagat gatcttgaga atgctgttat
      901 tgccactgag gataggactt tttacagtaa tagcggtatt aatcttaaac gcttcttatt
      961 ggcggtagtt acggcgggcc gctttggagg tggctcaacg attacacagc aactggctaa
     1021 aaatgcttat ctctcacaag atcagacaat taaacgaaag gccagagagt tttttttggc
     1081 gttagagttg accaaaaaat acagtaaaaa agatattctt actatgtacc ttaacaactc
     1141 ctactttgga aatggagttt ggggagttga agatgccagt caaaaatatt ttggaaccac
     1201 agctgctaac ttaacactgg atgaagctgc cacattagca ggtatgctca aaggacctga
     1261 aatatataac ccttaccatt ctctaaaaaa tgctactcac cgtagagata ctgttttagg
     1321 agcgatggtt gatgccaaaa agattaccca aacaaaagct caacaagcta gagcagtagg
     1381 gctaaaaaat cgcttagctg atacttatgt tggtaagaca gatgactaca aatacccatc
     1441 ctactttgat gctgttatta gtgaagcaat agcaacttat ggcctttcag aaaaagacat
     1501 tgttaataat ggatacaaag tttacaccga gctagatcaa aattaccaaa ctggcatgca
     1561 gacgactttt aacaacgatg aactatttcc tgtttcagct tatgacggta gctctgctca
     1621 agcagctagt gttgctttag atcctaaaac aggaggtgtt agaggtctga ttggtcgtgt
     1681 gaatagtagt gaaaatccga ctttcagaag ctttaactat gcgactcaag caaaacgtag
     1741 tcccgcatca acaatcaaac cactcgtggt ttacgcgcca gccgttgctt caggatggtc
     1801 aattgaaaaa gaactaccaa ataccgttca agatttcgat ggctatcagc cacataatta
     1861 tggaaattat gaatcagaag atgttcctat gtatcaagca ttagcaaact cttataatat
     1921 tccagcagtt tctacattga acgatatcgg aatcgataaa gcctttacct atggtaaaac
     1981 atttgggtta gatatgagct ctgccaaaaa agagttgggg gtagctttag gtggcagcgt
     2041 gacaaccaat ccattggaga tggctcaggc atatgctgcc tttgccaata atggagtaat
     2101 ccatcctgcg cacttgatta accggattga aaatgccagg ggtgaagtgc ttaaaacctt
     2161 tactgataag gctaaacgtg ttgtcagcca gtctgttgca gataagatga cagccatgat
     2221 gctaggtacc ttttcaaatg gaacagcagt caatgctaac gtatatggct atacactagc
     2281 tggtaaaaca gggacgacag aaaccaactt caatcccgac ttagcaggcg atcagtgggt
     2341 tattggttat acgccagatg ttgttattag tcaatgggta ggatttaatc agaccgatga
     2401 aaatcattat ctaacggatt caagtgcagg cacggcttca gctattttta gcactcaggc
     2461 atcttacatt ttgccttata ccaagggcag ccaatttcat gtagataatg cctacgctca
     2521 aaatggtatt tcagctgttt atggagtcaa tgaaacaggt aatcaatcag gagttgacac
     2581 tcaatctatt attgatggtt taagaaaatc agcacaagaa gcttcgcaat cactatcaaa
     2641 agcagtcgat cagtcagggt tacgtgataa agcccaatct atttggaaag agattgttga
     2701 ctattttaga tagcttgcag tattttagag atcgtgttaa aataatagtt acggaggcgt
     2761 tatggcacag aaaaaagcga gtctagcgtg tgtcgaatgt ggcagtcgta actactctat
     2821 cggagtgagt agcacgccta aaccaacacg tctagaagta aataaatttt gcaaatactg
     2881 caaaacatat accttacaca aagaaacacg ttaaaaagga gagcctgatg ggattcatta
     2941 gcgggacttt taaagtctta aaagacacaa catggccaaa tcgtaaacaa cgttggaaag
     3001 attttatctc agttcttgag tacacagctt tctttacggt gattatctac atctttgacc
     3061 agctcttagc aaagagtgta ttagcactga ttaatttatt ctaggtcaag attggtagaa
     3121 atgataagct tgcttggaaa agcctaggct gggagtattc ccagcctttt tgtagtatca
     3181 ataatctaat tagtaagctt ttttaaagcc catactggtt tgagctttaa aaaagcataa
     3241 aggtaaaggc aatgtagcaa cgatctccaa accaagttca ctaaattagc ttgtaacaga
     3301 gtcatcgcat aaacagcctg gcctcacttg cttagcacat taaaaatagc aatgaatatt
     3361 atggttgagg aaaacagcct gatagtctgc taaatcttcg ggatgataag ccttgacctc
     3421 agccatctca tactccctac ctagaagttt gtacttgttt tctccaaatt gatggaaagg
     3481 taacagttgc acttggtcaa tctcaagctg attaaagagc tctgagaagg ctttagcatc
     3541 gtctagggaa tcattaaatt ggggaataac agggatgcgc aagacgattt cttttccagc
     3601 ttgaaaggca taatgaatat ttttgataat caagtcatta cgaaccccag tcactttttg
     3661 gtgtctgagc tgattataat gtttcaaatc ggtatagata aagtccacgt agtcaataag
     3721 ggtgacaaat tgctcgtgtt tggcaaaggc ggtggtttca attgcggtgt gaagacctgc
     3781 tgctttggct gcttttaaca gggctaaggc aaagtcaaat tgggcaaaga tttccccccc
     3841 tgacagggtc atgcccccac ctgactcttc gtagaaatca aggtctttaa ggacttcttc
     3901 gatcacctcg tccacggttt tttcctctcc aactatttta gtattaagcc catcacttgt
     3961 taacatctgc tcaggtgctt tttgttggga ttcgggattg gcacaccatg gacatctcaa
     4021 ggggcaacct tttaaaaaaa cggttgtccg aataccagga ccgtcatgaa tactaaaatg
     4081 ttgaatgttg aaaacaatac cgcgatcagt cataagtgtg aattcctttc gttgtaaaac
     4141 catcatacca gataaacgta aataaatcaa ttacgaatga aagtaaatat cccttttctt
     4201 ttaagaaaat aactggtaca atgaaaggga ggtgaatgat gaatcgatta gaacgtatta
     4261 ttcaactggt ttcccagaag aaaaaaatag atgttaacag tttatcagaa caattagacg
     4321 tttcgaaagt aaccattcga aaagaccttg ataagcttga aagtaagggc ttgttgcgac
     4381 gtgaacatgg ctatgcggtg ctaaacagtg gcgatgacct caatgtacgc ttgtcctaca
     4441 attacaacat taagcgacgg attgcagaaa aagcagctga attggtgcaa gacaacgata
     4501 ccattatgat tgaatctggc tcaacctgtg cccttttggc agaagtgctt tgccagaaca
     4561 aacgaaatat taaaatcatt accaattcgt gcttcattgc caactacatt cgtcaatata
     4621 gtagctgtca aatcatttta ctgggcggtt attaccagcc caattcagag gtgactgttg
     4681 gtcctttatt gaaagaaatg atatcccttt ttcatgtcaa tcgtgtcttt gttggaacag
     4741 acggtttcaa caaagatctt ggctttatgg gcaaggacat gatgcgctca gaaggggtgc
     4801 gttacatggc agatgctgct gaggaagtgg tgattttgac cgattccagc aagttttcaa
     4861 aaaccagtct ggtacaccag ttgtcattag ctgatgtcaa tcgcgtcatt acagaccagg
     4921 cactagacaa gcaaacacaa gagttattaa gtgctagtgg tcttgttctt gattttgttt
     4981 cttagaaaat aggtggctag atgaaagaag aacgcagacg gctccttgcc aaggtggctt
     5041 acttgcatta tgttcaaggc aagagccaaa ctgtgatttc aaaggaaatg aatatctacc
     5101 gaacaacggt ttgtcggatg ttagcaaaag ccaaggaaga aggcattgtt cggattgaaa
     5161 ttgccgacta tgatgctgat ttatttgctt tagaagaata cgtgcgaaag cagtacggct
     5221 tggaaaaact agaccttgtg cccaaccagg ttgaagatac cccaatggat accttgacaa
     5281 atgttgctaa aacagcagct gaggttttta gacatgttgt taaagatggc gataagatcg
     5341 gtctgtcctg gggagcgact ctttcttgct tgatggatga gttaaatcca aaagcgatga
     5401 aagatgtgtt tatttatcct ttggcaggag gacctagcca tattaatgcc aaatatcatg
     5461 tcaataccct ggtttaccgc ttggctcgta tttttcatgg gaatagtgct tttatcaatg
     5521 caatggtgat tcaagaagat aaacaattga ccaagggtat tattcaatct aaatattttg
     5581 agagtatttt ggcgagttgg gaccagttag acctcgcttt agttggcatt ggaggagagc
     5641 ccaacagccg tgaggaaagt cagtggcgtg acttgttaac cgatgccgat tatgcacaac
     5701 tcaaacagga agaagctgtt ggagaagttt gctgccgctt ttttgatcaa gcgggtcatc
     5761 ctgtttacaa aggtttgcaa gatcggacga taggtatttc gctggaacaa ttaggtcgag
     5821 tgcgaaagac cattgccgtt gcaacaggta aatataaggc taaggcaata ttggcagcct
     5881 taaaagcagg ttttatcaat tatctagtga cggataaaga gaccatgcta gctgttttag
     5941 cgttagatga agatattgac cttaataacg ttcttcttta atcgaaactc aaagatacta
     6001 tgacattttt agaagctcga gatcaaatgg tcagggcttt ttttgttgtt tcgtttattg
     6061 ataaaacaga gattgctcaa ctgtgcaacg aacatattag cacaaaagtg cattaattaa
     6121 aatatagcac aaaatatatt gaccctagaa acaaacatga taaaatgaag ttatcaacaa
     6181 aaaagaaaac gattacaaac gtaattgaat cgttttcgta acaaagcgat agcatttgat
     6241 taagaggaga aggagttatt tatggaactg attgtaccag accaaatcat tatggggtta
     6301 attttaaatg ctggcgacgc taagcagcat atttaccagg ccttgaaatg tgccaaggct
     6361 gaagactatg acacgagtga aaaagaaatg gcattagcag atgatgcctt gttggaagct
     6421 cacaatttac agacccagtt tcttgcccaa gaagctagtg gtaataagtc agagattaca
     6481 gctcttttta ttcactcaca ggatcacttg atgacgacca ttacagaaat taatctcatc
     6541 aaagagatta ttgaccttcg taaagaattg gcaaccaaat aatatttcat tcataaaaag
     6601 gagattgata tgattaagat tggattgttt tgcgcagcag ggttctcaac aggcatgttg
     6661 gtgaataata tgaaagtagc tgctgaaaag aaaggcattg actgccagat tgatgcttat
     6721 tcccaaggaa agttggctga gtatgctcca ttgattgatg tggcactttt aggcccacag
     6781 gttgcctata ctctggataa atcagaagct atttgtaaag agaatggcat cccgattgca
     6841 gttatcccaa tggctgatta tggaatgtta gacggtaaca aggtgcttga tttagccctt
     6901 agccttgtta aagaatagga gtcaatcatg gcaaaaatga atatgcgaaa catcatcatg
     6961 ccaatcatga catttgtgaa tatgcgcggc atcattgcct taaaagacgg tatgttagcc
     7021 attttacctt tgaccgttgt ggggagtctt ttcctgattg ctgggcagat tcctttccaa
     7081 ggggttaatg atgccattgc tagtgtgttt ggtgctgact ggacagaacc ctttatgcag
     7141 gtttaccatg gaacatttgc tattatgggg ttgatttcct gttttgcaat tggctattct
     7201 tatgctaaaa actcaggtgt tgaacctctc ccttcagggg ttctatcctt gtcagccttc
     7261 tttattttat tgagatcatc ttatgttcca gcagaaggtg aagctattgg agatgctatc
     7321 agtaaggttt ggtttggtgg tcaagggatt attggtgcta ttgtgattgg tttaacggtc
     7381 ggagctgttt acacaacatt tatccgtcgt catatcgtca ttaaaatgcc agatcaagtg
     7441 ccacaagcca tcgccaaaca gtttgaagcc atgattccag cctttgtgat ctttaccttg
     7501 tcaatgcttg tgtatattat tgccaagtca gtaacaggtg gtggcacctt tattgaaatg
     7561 atttatgatg tcattcaggt accgctgcaa gggttaacgg gttctcttta tggcgcacta
     7621 ggtattgcct tctttatttc ctttctttgg tggtttggcg tgcatgggca atctgtggtc
     7681 aatggaattg tcactgctct tctcttatca aatctagatg ccaataaggc cttgatggca
     7741 gcaggtgagt tatccctaga caagggtgcc catattgtaa cccaacaatt tttagactct
     7801 ttcttgattc tctcagggtc aggaattacc tttggtttag tggtagctat gatctttgcg
     7861 gctaaatcca aacaatacaa ggctttaggt aaggttgcag ccttcccagc cctctttaat
     7921 gttaatgaac ctgtcgtctt tggttttcca attgtgatga atccagtcat gttcttgcca
     7981 tttatcttgg ttcctgtctt ggcagctctc acagtttatg gcgccattgc tattggtttc
     8041 atgcagccct ttgcaggagt gacccttccg tggtcaacac cagccattat ctcagggttc
     8101 atggttggtg gctggcaagg agctattgtg caaattctca tactcatcat gtcaacgttg
     8161 gtgtacttcc cattctttaa aattcaagat aatatggctt atcaaaatga acaagctagt
     8221 gaagagtcat gatttagcgt ccacacaaat tcttgagacc atctccagtt agaggtggtt
     8281 ttttagaaaa agaagagcga cttatcaggc ccatattgca caatagtgca aaataaagtg
     8341 actatctctt cttatggtta aggatacgca aaagccctaa tatatgataa aatagttacg
     8401 aaagaaacat ttataatttc gtaagaaaga aggagacccc gcatgactga aacaaaaagc
     8461 ccttattttg gacatttaac agacagaatg actcactatc gtgaagctgt gttagacaag
     8521 aaaccttata ttgatgctga gcgtgctatt ttagccactg aagcctatca aaaacatcag
     8581 aacaaaccag cgaacctgaa acgcgcttac atgcttcaaa ctattttgga aaacatgacc
     8641 atctatattg aggacgaaag cctcattgct ggtaaccaag cctcttcgaa caaggatgcg
     8701 ccgattttcc ctgaatatac tctggaattt gtcctcaacg aactcgatct ttttgaaaaa
     8761 cgagatgggg atgtgttcta cattaccgaa gaaaccaagc aacagctccg agacattgcc
     8821 ccattctggg aaaacaataa cctgcgtgct cgctgcggtg tcttgctacc agaagaagtg
     8881 caagtgtaca tggaaacagg tttctttggc atggaaggca agatgaactc tggggatgct
     8941 catttagcgg ttaattacca aaaactcttg gagcatggct taaagggatt tgaagaacgg
     9001 gcacgagctg ccaaagctgc gcttgatttg accattcctg aaaacattga caaataccat
     9061 ttttacgact ctgtttttat tgtcattgat gcagtcaaaa cttatgctaa acgctatgcc
     9121 aagttggcta gagaactagc aaaaacggct aaaccagaac gtcaagcaga attgttagac
     9181 atcgcgcgca tttgtgataa agtaccttac gaaccagcga aaacctttgc agaggctgtg
     9241 caatctgtct ggttcattca gtgtattttg caaattgaat caaatgggca ttccctatct
     9301 tatggccgct ttgaccagta catgtatcct tatgtcaaag ctgatttgga ggctggccgt
     9361 gaaacagaag acacaattgt tgaacgtttg accaacctct ggattaagac cttgaccatt
     9421 aataaagtac ggagccaagc ccacaccttc tcatcagcag gtagtccact ttaccaaaac
     9481 gtgacaatcg gcggtcaaac acgcgataaa aaagatgcgg tcaacccact gtcttacctt
     9541 gtcctacgga gtgtggctca aaccaaattg ccacaaccta acttgactgt tcgttaccac
     9601 aaaggcctag acaatacctt tatgaacgaa tgtatcgagg tcatgaaact tggttttggt
     9661 atgccagcca tgaacaacga tgaaatcatc attccatctt tcatcaaaaa aggtgtctca
     9721 gaagaagatg cttatgatta ctcagccatt ggctgtgtgg aaacagcagt tcctggaaaa
     9781 tggggctacc gttgcacggg gatgagttac atcaacttcc ctaaaatctt gctgattacc
     9841 atgaatgacg ggattgaccc agcatcaggc aagcgctttg caaaaggaca cggtcacttc
     9901 aaagacatga cctcttatga agaattaaaa gcagcatggg atgccaccct gcgcgaaatt
     9961 acccgcatga gcgtcattgt tgaaaatgcc attgacctcg gtcttgaacg tgaagtacca
    10021 gatattcttt gctcagcctt gacagatgat tgtatcggtc gtggcaagac cttaaaagaa
    10081 ggcggagctg tttacgacta tatctcaggc cttcaagttg gtattgccaa cctatcagac
    10141 tccctagcag cgctgaaaaa attagtgttt gaagaaggtc gcttgacccc agaggaactc
    10201 tggaaggccc ttgaaagtga ctttgctgga gaacgcggag aagacatccg ccaaatgctc
    10261 atcaatgacg caccaaaata tggtaatgat gatgactacg cagatagttt agtcgtcgaa
    10321 gcttacgaca cctacattga tgaaattgcc aaatacccaa atacccggta tggccgtggc
    10381 ccaatcggag gtatccgcta ttctgggaca tcatcaatct cagccaatgt cggacaagga
    10441 aaagggacct tggcgacacc agacggtcgc catgcaggca caccactagc agaaggctgt
    10501 tcaccagaac acagcatgga caaaaaaggc ccgacttcag tattgaaatc tgttgctaaa
    10561 ttaccaacag atgagattgt cggaggagtg ctactcaacc aaaaagttaa cccacaaacc
    10621 ctggccaaag aagaagacaa actgaaatta atggccttac ttcgtacctt ctttaatcgc
    10681 ctacacggtt atcatatcca gtacaatgtc gtgtcacgtg aaaccttgat tgatgcccaa
    10741 aaacatcctg aaaagcaccg cgacctcatt gtccgtgttg ccggctactc agctttcttc
    10801 aatgtgctct caaaagcaac ccaagatgac attattgaac gtacagaaca cacactttag
    10861 gagaaaataa gtatggaata catgttagac actttagatt tagaagctat taaaaaatgg
    10921 catcacattt tgcctctcgc aggtgtgacc tcaaaccctt ctattgccaa aaaagaaggt
    10981 gagattgatt tctttgaacg gattcgagaa gtccgtgcta ttatcggtga caaggcttct
    11041 attcatgttc aagtgatcgc ccaagattac gaaggcatct taaaagatgc tgctgaaatc
    11101 cgcaggcaat gtggagatag tgtgtatgtc aaagtacctg taaccacaga gggccttgca
    11161 gccattaaaa ccttgaaagc cgaaggctat cacatcacag caaccgctat ttatacgacc
    11221 ttccaaggac tattagccat tgaagctggc gctgattatt tggctcctta ctataaccgt
    11281 atggaaaatc ttaatattga tccagaagct gtcatagaac aattagcaga agctattaac
    11341 cgcgagaatg ctaatagtaa gattttggca gcaagcttta aaaatgtggc tcaggttaat
    11401 aaatcctttg ctctaggagc acaagccatc actgctggcc cagatgtgtt tgaagcagga
    11461 tttgccatgc catctattca aaaagcggtt gacgattttg gcaaggattg ggaagccatt
    11521 catcaccgca agagcattta agaaatagca tcagtagtaa tcacatcatt aggaatcaca
    11581 gataaaggag ttcaacgatg aaagtatttg caagtccatc tcgttacatt caaggtaaaa
    11641 atgccttgtt taccaatgtt aaaaccctaa aacaactagg ggatagcccc attttactgt
    11701 gtgatgacgt ggtgtatggc atcgtcggag aaagatttga aagctatttg atagataatg
    11761 gcatgactcc tgttcacgta gcctttaatg gcgaagcctc agataatgaa atcagccgcg
    11821 tggttgccat tgccaaggaa aatggcaatg atgttattat tggacttggt ggtggaaaaa
    11881 ccattgacag tgctaaggct atcgctgact tacttgctgt tccggtgatt attgccccaa
    11941 ccattgcttc aacagatgcc ccaacctcag ccttatcggt tatttacacc gatgaaggag
    12001 cctttgaaaa atacattttc tattcaaaga atccagatct tgtattggtt gacacacaag
    12061 tgatctgtca ggcaccaaaa cgcttattgg cgtcagggat tgcagatggg ttagcaacat
    12121 gggtagaggc gcgtgctgtt atgcaaaaaa atggagacac catggcaggt ggcaatcaaa
    12181 ccttggcagg agttgccatt gccaaagcct gtgagcagac cttgtttgca gatggcctaa
    12241 aagcgatggc tagctgtgac agacaagtcg tgaccccggc cttggaaaat gtcattgaag
    12301 ccaatacact tcttagtggg cttggttttg aaagtgcggg tctggctgct gcacatgcca
    12361 ttcacaatgg ctttacagcg ctgacaggtg ctattcatca ccttacccat ggtgaaaaag
    12421 tcgcctatgg cacgttgacc caactcttct tggaaaatcg ttcgcgtgaa gaaattgacc
    12481 gctacatcga cttttaccaa gctattggca tgccaacaac cctcaaagaa atgcatttag
    12541 acacggctac tcaagaggat ttcttaaaaa ttggccgtca agcaaccatg gcaggcgaaa
    12601 ccatccatca aatgccattt gttatcagtc cagaagatgt agcagcggct ttggttgctg
    12661 ttgatgccta tgttacgagt cgataggcta accaaaaaga gatttcttat ttgccagata
    12721 acctcaaaaa aacaagtcta gggagtcact tcctagactt gttttgctta ttgttggttg
    12781 gtaatagcac ttttagcggg gtcattgtgg gcaatacgac tggcagctgc tgccgcaatg
    12841 gcgctaacaa tatcatctaa aaaggtatgg caggagtagc catctttgtg gttgagtttg
    12901 tcaactattc ccggttttgt cttgtccaga tacccataat tggtaaaacc gatagaccca
    12961 tagaggttaa caatggataa ggccaggatt tcatcaatcc catacagccc ttggtcggtt
    13021 ttcaagatac taaggagtgg ctcagacagc tggttggctt cagccaattt atccaattct
    13081 accccagtga taatagcatt ttgcacttct cgttttgcaa gcaccgcctc aacgctctct
    13141 aaacattctg ccatggtcaa attgggaata taatcattct gcaaaaaaag caccagctct
    13201 gcaatagctt ctaaagaaac ccctcgctca gccagtaatt gataagaaac ttctcgtaat
    13261 tgatggtcag ttgacatgtg aaacctcctt tttttcttta ttgtaacata aaagatttta
    13321 agaaacctct acaacagcta aagagggaat agttcttttt taagaggtgt tgactactga
    13381 aatcccctac ttggtaaaag tcaaagaaac gatagtaaag taaccatctt acctaggcct
    13441 aaatacaatc accacctcta ggtgacacac agcacctatt tcaaaaatga tattagtatg
    13501 gcaaaacaaa aagacaacgc cttctttttt ctccttacta tctcctttaa ttttcatatt
    13561 ttttaaaaaa actattgata aactagttaa gtaagcgtat actatggtta gtgagcgaaa
    13621 ttagaaaaga ggacaagcat atgaatctac ttggatcaag acgggttttt tctaaaaaat
    13681 gtcggctagt aaaattttca atggtagctc ttgtatcagc cacaatggct gtaacaacag
    13741 tcacacttga aaatactgca ctggcacgac aaacacaggt ctcaaatgat gttgttctaa
    13801 atgatggcgc aagcaagtac ctaaacgaag cattagcttg gacattcaat gacagtccca
    13861 actattacaa aaccttaggt actagtcaga tcactccagc actctttcct aaagcaggag
    13921 atattctcta tagcaaatta gatgagttag gaaggacgcg tactgctaga ggtacattga
    13981 cttatgccaa tgttgaaggt agctacggtg ttagacaatc tttcggtaaa aatcaaaacc
    14041 ccgcaggctg gactggaaac cctaatcatg tcaaatataa aattgaatgg ttaaatggtc
    14101 tatcttatgt cggagatttc tggaatagaa gtcatctcat tgcagatagt ctcggtggag
    14161 atgcactcag agtcaatgcc gttacaggga cacgtaccca aaatgtagga ggtcgtgacc
    14221 aaaaaggcgg catgcgctat accgaacaaa gagctcaaga atggttagaa gcaaatcgtg
    14281 atggctatct ttattatgaa gctgctccaa tctataacgc agacgagttg attccaagag
    14341 ctgtcgtggt atcaatgcaa tcttctgata ataccatcaa cgagaaagta ttagtttaca
    14401 acacagctaa tggctacacc attaactacc ataacggtac acctactcag aaataatacc
    14461 aaaaggctag acctctgctc actaggccta gctttttaca tcaaaaaaag caatgactat
    14521 agaaagtaaa aatactagaa aaagcaatga ttgtcgtcat tgctttttat gatttgtcaa
    14581 aaagtaaaaa gcaaattgaa aaaatcgccc tggagctgtt gagataaact acaccattta
    14641 ttaagatacc aaatcaaaaa gctaacacca taagagcgtt agctttttaa agagcctcag
    14701 gacagtttat gtttaatggc ttctaggtag gtttgaaaca tatgatggat cgttttgcaa
    14761 ttgagtaatt ggaagatctc caagatctgc acacactttt gctcagcccc ctctttacca
    14821 tttatgaggc caataatgcc atcaaaaaaa gacacacaac accgttcata taagcaatca
    14881 tcatttagct gataatcttc aatttttgcc aaaatctcag aagctttatc atactcttgt
    14941 cgctgaataa acaaaatcaa catattgaca aacatccgaa tcgattcatt cccataatac
    15001 cttagggtat tgtatttatc gaggttcaaa ataacttttg aaaacaccat ctcaataaag
    15061 caagactcaa aaataaacat acaattatta aaaagcacag tctcatagtg actccaagtt
    15121 tcaatattaa taagatagtt tgacaaataa gtccgctctg tcagacagtc ttcctcagta
    15181 agagttgcta ataacacctt gaccaaggca aaaaggttct tttcctttgt tgacttacta
    15241 tctttataag aatcaaggat atgatttaaa ccttctatgt ttcggcattc aaaataatgt
    15301 tttgccgtat ccatatcaat aaattcttta tattgtttaa agttattact aataaagaga
    15361 aactcatcaa cattgacatt caagttgtct aagatataaa gtaacttttt cgaagaaatg
    15421 tcaagattat ttttaataaa acgataatag gtttgcctag tgagataatc accacaaact
    15481 tgtttaatcg aaatgttttt tgaatgccta atgaattcaa cggtttcacc aatttccata
    15541 tgtcaagcct tcctagttga tgtcaaaaat acgttacgca tgtacctaat acgtaacaag
    15601 ttgaatgttt cggatgatag tcgcttatga taggtgcata aggtcaatag ccagatgcga
    15661 tagttttatc aactgtcata tgttaaacct ttctaatcta atagatgtta aaaatacgtt
    15721 acgtgtgtac ctaatacgta acaaaataat gggttagcaa aataagcagc tatgatatag
    15781 ccataaggtt aaaaggaggc gcctactatg tggttattgt tactattttt gtagttcctt
    15841 ttgcaaaatg gtagtagata aaaatgagaa gtcaatagaa gcattgacaa acaaaagaat
    15901 cattttgttt atattatcag tatttttaag tttcttgtca actgaaatga gcatctacta
    15961 gccacaatag taactaaaag gctaattgtc agtaaaaatg agataaatca gccaggaaaa
    16021 agcctacgag tcaactaatg gacagcccaa aaaaagacaa aggattttca cttggtgttg
    16081 tggaccctat cacgatcctt atcacagaat cagttattaa caaaatgaga acggtcccat
    16141 atcatctaaa aaaactgtca agcacaacct attgtttgct agctgatctt gcctatcagt
    16201 ctgcctagta gatacggcag aagataagag atcaaccaaa agatgataag tgcaacgact
    16261 catagcgtct tttgaccaaa accaattaga ctcacatgag atgagaataa ttgggttggg
    16321 ttgtcagtgt caactaaccg tgttattgtc tattaccatt catggtatca gcgacatcgt
    16381 atgataacca tacgattcag ctaagtaagg aggtgtgtcc aatgtaccgt taaaagcaaa
    16441 tgcagtagat taacttattt tgaaagaggt ataaaaaaaa tgaataaaaa gaaattaggt
    16501 atcagattat taagtctttt agcattaggt ggatttgttc ttgctaaccc agtatttgcc
    16561 gatcaaaact ttgctcgtaa cgaaaaagaa gcaaaagata gcgctatcac atttatccaa
    16621 aaatcagcag ctatcaaagc aggtgcacga agcgcagaag atattaagct tgacaaagtt
    16681 aacttaggtg gagaactttc tagctctaat atgtatgttt acaatatttc tactggagga
    16741 tttgttatcg tttcaggaga taaacgttct ccagaaattc taggatactc taccagcgga
    16801 tcatttgacg ttaacggtaa agaaaacatt gcttccttca tggaaagtta tgtcgaacaa
    16861 atcaaagaaa acaaaaaatt agacactact tatgctggta ccgctgagat taaacaacca
    16921 gttgttaaat ctctccttga ttcaaaaggc attcattaca atcaaggtaa cccttacaac
    16981 ctattgacac ctgttattga aaaagtaaaa ccaggtgaac aatcttttgt aggtcaacat
    17041 gcagctacag gatgtgttgc tactgcaact gctcaaatta tgaaatatca taattatcct
    17101 aataaagggt tgaaagacta cacttacaca ctaagctcaa ataacccata tttcaaccat
    17161 cctaagaact tgtttgcagc tatctctact agacaataca actggaacaa catcctacct
    17221 acttatagcg gaagagaatc taacgttcaa aaaatggcga tttcagaatt gatggctgat
    17281 gttggtattt cagtagacat ggattatggt ccatctagtg gttctgcagg tagctctcgt
    17341 gttcaaagag ccttgaaaga aaactttggc tacaaccaat ctgttcacca aatcaaccgt
    17401 agcgacttta gcaaacaaga ttgggaagca caaattgaca aagaattatc tcaaaaccaa
    17461 ccagtatact accaaggtgt cggtaaagta ggcggacatg cctttgttat cgatggtgct
    17521 gacggacgta acttctacca tgttaactgg ggttggggtg gagtctctga cggcttcttc
    17581 cgtcttgacg cactaaaccc ttcagctctt ggtactggtg gcggcgcagg cggcttcaac
    17641 ggttaccaaa gtgctgttgt aggcatcaaa ccttagtatg gaaatgcatt tcgttagaac
    17701 agaacctgag gcacgccgca tagctgaaac cttttgtgcc gaaaacacac aaacaaaaac
    17761 ccctatgcgc gtgcagcagt taagctatcc atcagacaca gatcactcaa gtggtgagct
    17821 atacatctat gctttgtctc ctgctggatt tatcatcgta tcaggagaca ccagagcgca
    17881 caccatttta ggctattctt ttgataataa cctagacctc aaccatgata atgtcaggag
    17941 tatgatagaa gcttaccaaa aacaaatcag ctctttggat tgatcggtca aagtagcttt
    18001 tgccccctca ccactcgttt aagacgaccg tgtttctaaa ataccactac cttttaagtt
    18061 tcacgccggc ctaagcgagt gtttgtctca tggagcagac tgtttccatc gaaaagtcac
    18121 ccattgataa aggaggtcat caacgcctac taatgatgac ttccctaaga tgccaaggta
    18181 aaggactgag ttctgtagag atgggactca gtccttttac gatttttggg agttattcta
    18241 gccataccgt ggcaagaaaa caacacttaa aaatagccaa cgtcagtttc tgttcataca
    18301 tgactacagc caaacaccac aacctaacct atcctaaaca cagtggcttt ctaacgaaca
    18361 gtcacaacga gcagcaacac caaaaagcaa gattaagcta cgtttaagac taatgatata
    18421 agataacagt aattattaat gagaaaagag gctcaaaagg acatgaaaca aatgaataaa
    18481 ctcattacag gagtggtaac gctggcgaca gtcgtgacct tatcagcttg tcaatcatca
    18541 cacaacaaca ccaaactcgt ctcgatgaaa ggagacacca tcacagtcgg tgacttctac
    18601 aatgagacca aaaacacaga actcgcacaa aaagccatgt taagcttggt gattagccgt
    18661 gtatttgaga cacaatatgc caacaaagtc tctgacaaag aggttgaaaa agcctataaa
    18721 caaaccgcag accaatacgg tacatccttt aagacagtcc tagcacaatc aggcttaaca
    18781 ccagaaacct ataaaaaaca aattcgcctc acaaaattag tcgaatatgc cgtcaaagaa
    18841 caagccaaaa acgaaaccat ctcaaaaaaa gactaccgtc aggcctatga cgcttatacc
    18901 ccaaccatga ccgcagaaat catgcagttt gaaaaagaag aggatgccaa agcagccctt
    18961 gaagccgtca aagctgaagg ggcagacttt gcagctattg ccaaagaaaa aaccactgca
    19021 gccgataaaa aaacaaccta cacgtttgac tcaggcgaaa caaccctacc agcagaagta
    19081 gttagagctg catcaggcct caaagaaggg aacagatcag aaatcatcac agcgcttgat
    19141 ccagccacct caaaacgcac ctaccatatc atcaaagtca ccaaaaaagc aactaaaaaa
    19201 gcagactgga aagcgtacca aaaacgcttg aaagacatca tcgtgactgg caaattaaaa
    19261 gaccctgact tccaaaacaa agtcatcgct aaagctcttg ataaagcaaa tgtcaaaatc
    19321 aaagacaaag catttgccaa tatcttagcc cagtttgcaa aacctaacca aaaacaacct
    19381 gcccaaaaat agagggttgc cagtttattt ccctaaggtt gcttaaaaag acagccctag
    19441 ccatcactcc ccttttcaaa acaccgataa tggaaacgtg tcctatactg atggtctagg
    19501 gcaatgtcat aaagttaaag actctatcca aataaggata gagtctttaa tatttaaaaa
    19561 ccagtcagca aaaaaaaatt cacgctaagc ctagctagaa tgaggcttag cgtgaatggt
    19621 atcagtgata gtccgttaac atagctgatt gggagtcaga aactctaact acccaatctg
    19681 actatacccc cagtaccaga ggtgtctcat cgcagaagta gcaatcagat aatcgtctga
    19741 taagctcttg tcctcaagca actgatgaat atcgtcgcat gggccatgag ctgtcggtgg
    19801 ctgatagcta gtaggctcat cagccaaaac ttttttggag taacgctttg gtctcagacg
    19861 gcgccagagg gttgagagag gggtgagaaa aaaacgatgg gtcatcttgg cctccttgtc
    19921 taatgatagt caccttaaca gacaaaagtg tcaataatgc cattttaagg agaaaaagat
    19981 taaaaatcaa ggtcacaaac tttcctttgt taaaaaaaca cgaccctaat aaagaaccta
    20041 aatactcctc gcacttgagc ttctccttgc gcagctatct tgcgcactca gttgcacaca
    20101 agctaatgat agcgatacca ttattgtcct atacgctagc gagacaacct cttttttcgc
    20161 ctcaacgagg ccaacacagc tgtcatgaca gagaaaagac ctttgtctat ctaagggacc
    20221 attaattttt ctctcaccaa aaaactgatt ttagaaacga agaagcatgg tgtataataa
    20281 ggttcggaac aattatgaca taataatgaa agtaaggtta acgaaacatt gacaaattgt
    20341 aagtataaac ttagaaagtt atctgtaggg ctcgtctccg tcggaacgat gctgatagcc
    20401 ccgacagttt taggacagga ggttagtact actggtagta ctgagacgag tgctgctagt
    20461 accgctagcg tagacgctac gacaagtggg acgactgcta gcggagctag tggtgaatct
    20521 agtgatgcgt ccgtagcatc tagtgaggga agtcagggtt caaaatctgc cccagcctca
    20581 cctcaacctc aacctcaagc acagacagct ccagcagcaa cgtcggcatc tagtaaagcg
    20641 aaaactgaag agcaaactcc taaggcagca acttcatcca caccatcgac tccagcagcc
    20701 agtagcagta gtaatagcaa tcaagaagct agtgctgaga ctgaaccaca gatgatggac
    20761 gtggaaaagt atacagttga taaggaaagt tcagagctta aggttaaaga cggtaagaag
    20821 cccaaaaacg agaataaggt tgataaagat acaaaactta ttagaaaccg tgatggcgag
    20881 cagcgtgata tttttgatat caagcgtgag gtaaaaacaa acgcagatgg cactatcgat
    20941 gttacggtta ccgtaacgcc taaagaaatt gataaaggtg ccgatgttat ggccctttta
    21001 gacgtctctc aaaagatgac aaaagagaac tttgataagg ctaaagaaca aataaaaaaa
    21061 atggttacga ctttaacagg cgagccaact gatggtaagg aaaatcgtaa taggcgtaat
    21121 tctgtacgtc taatgacctt ctaccgtaaa atcagtgaac caattgattt atcaggaaaa
    21181 actagtgaag aagtcgaaaa agaattagat aatatatggg ataaagtcaa aaaagaagat
    21241 tgggactggg gggttgattt acaaggtgct atccataagg ctcgagacat ttttaagaaa
    21301 gaaaaagagt caaaaaaacg ccaacatatc gtcttgttct ctcaaggcga gtcaaccttt
    21361 agttatgata tcaacgacaa agataaaaat aatactgtca ggaaaaacag aataactgga
    21421 aaagttacga cctctaaccc actatttccc tggctaccta tatttaatca tacaaatcaa
    21481 aaagcagaag ttattgatga tgtagacaaa ttactggatt tcgctgaaaa aatgggaata
    21541 tcactgccca agggattaag agcaggagtg caggctattg ggttaagtaa tagtttcctt
    21601 agtaccttca caggatctgg attgacagag tacctcactt tgaatgaata tggctcagat
    21661 atactaaaag aaaaacagtt cgattatacc aagcgtgtag gtgagggcta ctactaccac
    21721 agttattcaa agcgaactca tggagataag atgccctttg agaaacaaat aagagaagtt
    21781 ttagagcaat ttttaccgaa aatcgaaagt agagaatggg ctaaaaagtt tatagacatt
    21841 tttggattac aaggacaaaa agtagatcaa attggggtag atgtaattat gaaagtaatt
    21901 aacagcatct tctacagtcg ccaatactac tactataatc acaacctgtc agcgatagct
    21961 gaagctaaaa tggctcaaga agaaggtatc accttctact ccgttgatgt tactgattta
    22021 aaacctaaaa gagcgaagcg acaagcagca gtacttcaga aaaccacgaa aaaagaatcc
    22081 gaagaagacc gtaataacaa atttgatggg taccttaaaa agatgtctga aggcggtaaa
    22141 gaatttttta acgatgttga tgaggcagat aaattcaaag atactttaac tgaagtaacg
    22201 gtgacagaaa cttttgaaaa tagtgtatct gttgagaatc aatcttggaa ggtttcatca
    22261 aatagtaacc acaaagagaa tgttaaatac acaaatgctc agaaaaacag aaattggttt
    22321 agttctgagg taaaagaaag cctcacttgg actatttcca aagagcagtt gaaacaagcc
    22381 tttgaagatg gaaaaccgtt aaccctcacc tataagctta aggtggataa caacaagctt
    22441 aaaaaagctc tcgacgataa gagaaagaat agaccaaaac gttctatacc tacagaaaat
    22501 gaaaactctg tcacagaaaa aatcatttca aatactgtta actacaaaat taataagcaa
    22561 gaagttaagg gtaacaaact tgatgatgtc aagttgactt atactaaaga gaccgttcct
    22621 gttccagatg tggaaggaga agttgtacca ataccagaaa aaccactggt agaaccaatg
    22681 acgcctctat accctgcaat tcctaattac cctacccctc aacttccaaa agatgaagat
    22741 ctggagatta gtggaggtca tggaccgatt gtcgatatcg tcgaagatac tggtacaggt
    22801 gttgagggcg gcgctcaaaa cggcgtggtt tcaactcagg aaaataaaga tccaatcgtt
    22861 gacatcaccg aagataccca accaggcatg tcaggctcaa atgacgcgac agttgtcgag
    22921 gaagacacag cacctaaacg tccagatatc cttgttggcg gtcaaagtga tccaatcgat
    22981 atcaccgaag atactcaacc aggcatgtca ggctctaatg atgctactgt tatcgaagaa
    23041 gatacgaaac caaaacgctt cttccacttt gataacgagc cacaagcacc agaaaaacct
    23101 aaagagcaac catctctcag cttaccacaa gctccagtct ataaggcagc tcatcacttg
    23161 cctgcatctg gagacaaacg tgaagcatcc tttacaattg ttgctctaac aattattgga
    23221 gctgcaggtt tgctcagcaa aaaacgtcgc gacaccgaag aaaactaacc ctcgttagtc
    23281 tagcgaccct aagcctttgg ctttaaaaat ttctcattat ccccctcaaa caccggtacc
    23341 tcactgagat ggcgcttaaa aaagccgcta gtctcaaatc aggtatcccc tacggttgag
    23401 ggggattaat gggtatcaca acaagtaaaa aggtataatg atgaaatatg actaaaaaat
    23461 ttattaaatc agcagtgatt ctaggcttag caagcataac catcagcatg acgcaacccg
    23521 ttgaagccat tgcaaataac tcaaaatatc aagcacaata cgaacttgtc aaaaaagaac
    23581 tagacgcatt aaaagcaaca agtccaaaat taggagaacg ttccctttat tttagattgg
    23641 tgaataaatt tctaagttgg tttggccaag tctgggaccc actcgcaatc aaagaaaccg
    23701 attacctgtt ggacctcatc ggacaagtca attacttggt gagtaacacc gagtttcaag
    23761 cccctgagta caaagatgct aaaaacaaac tagatttagc tgtagcaggc tatgagctct
    23821 acctagctag agaaaaagaa cgtggtgatg ctcctgatta ccaagtgacc cagctaacgc
    23881 tagatcagct cgaaaccatc aaaacgaaga tacaaaaggc tctcgaaaca tttgcacaag
    23941 aggtcaaaac aatccaacaa acacacccgg acctatttcc ccatgcttat gttttaaacg
    24001 ctaagagcaa agatatcaca gataaagcgc aacccttttt agtgaatggt tactacgatg
    24061 tcttttataa gcagttttta ttcactaata caaatgacgg gtataactat aataaagaag
    24121 cagacaacct ggcctcgtat caaaatctga ttaaagagct attaacattg aaagctgaga
    24181 tcgatcagtt aacaaaaggc aagactggcg atgaccgttt agagacgtta atgtgggaat
    24241 caaagttcac cttatctgac tttcggagaa cttcacgcct tagcgcctac tatacacaag
    24301 aagattatgt caaaaaggtg gtcctatctg aagaagctcc caacttgaga acagcaaaag
    24361 aaaaggtcaa gaccttaaaa gggatcttgc atgattacta tgttgacatt aaagagccag
    24421 aaaaagctaa aaagtatcat gtcgaggacg aaacctcacc gtctcaacct gaggcccctg
    24481 ctaaacctga agctcctagt cctggtcctg ctcctggtca aaaaccagca gaagatgagg
    24541 gatcgtcaca agctatggag ccagctaacc cgtcaaaaga ggattcctca acggacgcat
    24601 ctcagggatc tcacgatagt gaaaacccag cgacagacag tccttctcaa cctcaggcac
    24661 cagaccaagg caatcaccaa agccaagtgc caaacgccaa gcctcaaacg gacaagacgg
    24721 acactcctaa cgtcccagcc cctccacagg acaccccaaa agtgcctgag gctggtggtc
    24781 agagtggccc cgctgggaac gctgaggaga aagctcctga ctctggacct aaagagtcag
    24841 gtcaaagctc gtctaaagag tccccatccg ttggcgaaga cacgacccct aatcagccag
    24901 acgtccttgt aggtggtcaa agtgagccaa ttgatatcac cgaagacaca atcactgacg
    24961 ctcctccgac agtatctggg cacaatgcat caacacagcc ccaatctgta gtcgaagata
    25021 cggcatctca gcgcccagat gtccttgtag gtggtcaaag cgagccaatc gatatcaccc
    25081 aagataccca accgggcatg tcaggctcta atgacgctac cgttatcaat gaagatacaa
    25141 aaccaaaacg cgtcttccac tttgataaca aagagccaca agcatcagaa aaagcggcag
    25201 agcaaaaact tgctcttgct cctcatgata gccacacaac tcctcaagct tcagatgata
    25261 cagcagcccc tcacttgcct gcatctggag ataagcctga aaaagtgttt gcacttgctg
    25321 ccttgacaat ggtcgcagct gcagggctac tcagcaaaaa acgtcgcaac accgacaaaa
    25381 actagtcgct gttaggcaca acgcatcact aaggccagtg caaatgcact ggccttagtt
    25441 aggtttttat caaatagagg gtttttcatg acaattgcta gcgtttcggt gtaaaataaa
    25501 ggtaataacc agcaaaggag caacctatga caaagcaaga tcagttaatc gttgaaaaag
    25561 tggaacaaac ctatgaggcc tttagcccaa agctagcaaa tctcatagaa gccctagatg
    25621 cctttaaaga gcactatgag gaatatgcca cacttagaaa tttttacagt agtgacgaat
    25681 ggtttcggct tgcaaaccaa ccttgggatg atatcccgtg cggtgtccta tctgaagacc
    25741 tcctctttga tatgatcggt gaccataacc aactactagc tgatatctta gacttggccc
    25801 ctatcatgta caagcatatg taaccattgt taacgtcaca gatactacga tgacaggaca
    25861 aaaaaagctt gaaaatctca aaaaaaacag ataaaaaaca gataatcatt agaaaaacag
    25921 tagatgcgtc tgttaaacta aaggtatcaa aacaaaagga gcatttcatc atgaaattca
    25981 aaaaagtatt agtcattcct gcattagcat tagcagcaac atgcttttta acagcttgtg
    26041 gcacaaaaaa agattctaaa aaagaagaag ttaaagaaat caaaatgtct gacatcaaag
    26101 acgacgcagt tagcaaaaaa acaaaagtgg ttgatggcga agaagttaca gaatacacca
    26161 caaaagatgg aaacgtgatt cagattcctg caggaaacga agaaggcatg gaatcaaaag
    26221 atgctggtgg atcaggcgct cctgctaaaa actaagggtt acaacaaaga gcataaggtg
    26281 actcgcaaac gttacgcatg acatgaggca gtctgcctaa gatggtaact cacttatcat
    26341 aatgcctaaa tagccaaaca cactaactcg caaatgtaca aaatcatata cccgtgctta
    26401 tgtgagcgaa cggttatatg atttttgttt tccaaaaaca agaaaagaac gctaaaatag
    26461 ccagtttgcc gtgtttcaag ctaccaaaaa agaagtgcta aaatgaaaaa aagatccatt
    26521 atacctgtca tcttactgtt ggtcatcgga ttaatcgttg gtgttaagcg tcatcatgac
    26581 tagtaggtct tgctagtgac cgttcaaaag ggataacgtt ggtatggtat aaccataaaa
    26641 atcgtataaa aagaaaaaat agctattgag gagaaagtat gtttcagtta agaaaaaaaa
    26701 tgacgcgcaa acaattagcc ttgttgagtg ctggagtgtt gacctgtgtg gttggtggta
    26761 gctacttgat aatgaaccat caacaacaag aaattgtctc tagtgtcaac aaagtaaaag
    26821 ccttaaccat aaaagaagcc atggaacaag gaaaagatat cagcttgacc ttagctggcg
    26881 aagtaacagc taacaacagc agcaaagtca aaatcgactc aagtaaagga gaagtcaaag
    26941 atgtctttgt caaaaaaggc gatgttgtca aagtaggaca acccttgttt agctatgaaa
    27001 cgtcacaacg gttaacggct caaagttcag aatttgatgt tcaaaccaaa gccaatcaac
    27061 ttcaagttgc taaaaccaat gcagcattga agtgggaaac ctacaatcgc aaggtcaatg
    27121 aaatcaacac cctaaaatct cgctacaaca ctgcaccaga tgagagctta ctagagcaaa
    27181 ttcgcagcgc agaagacagt gtatctcaag cactaagcga tgccaaaaca gcagatagcg
    27241 atgtcaaaac cgctcaaatc gaactcgata aagctaatgc tactgccaca acggaaaaag
    27301 gtaaactaga gtatgacacc gttaagtcag acaccgcagg aaccattgtt agtctaaata
    27361 ctgatttgcc aaatcaatca aaatccaaaa aagaaaatga aacttttatg gaaattatcg
    27421 acaaatcaaa aatgttagtc aaaggtaaca ttagtgaatt tgaccgtgac aagttaaaaa
    27481 tcggtcaaaa agtcgaagtg attgaccgca aagacaactc taaaaaatgg actggaaaag
    27541 taacccaagt tggcaacctc aaagcagagg aaaaaggcca aggtcaaggc caaggtggca
    27601 atgaccaaca agataatcca aaccaagcaa aattccctta tgttattgaa cttgaccaat
    27661 cagacaagca gccactcatt ggctcacaca cctatgttaa tgtgctcaac aatgttccag
    27721 aagctggcaa gatcgtattg aaagaaacct ttacaatggc agaaaatgga aaaatctatg
    27781 tgtggaaagt tgataaaaac aaggtcaaaa aacaagaaat caagactaag cccttctcaa
    27841 aaggttatgt tgaggtaaca agtggcttga ctatgcaaga taagattgct cagccgcttc
    27901 ctggcatgaa agacggtatg gaggtaggaa gtattgttaa accttaaaga tattcgaaaa
    27961 agctatcatc ttggaactga agaatttgcg attttaaaag gaatcgattt agaagttaac
    28021 gagggtgact ttttagccat catgggacca tcaggttcgg gaaagtcaac attgatgaat
    28081 atcattgggt gtttagataa gcctggctct ggctcatatg ccattgaagg cagagacgtg
    28141 tcatccttat ctgataatga acttgctgat ttgcgtaatc aaaaaatcgg ctttgttttt
    28201 caaaatttta acctgatgcc caagctaaca gcttgtcaaa atgtcgaatt acccttgact
    28261 tatatgaatg ttcctaaaaa agagcgtcgc aaacgagccc tagagatgtt aaagctagta
    28321 ggactagaag aacgtagtga atttaaaccg atggagctat ctggtgggca aaaacagcgt
    28381 gtagcgattg caagagcttt agttactaac ccgagtttta tccttggtga tgagccaaca
    28441 ggtgcactag acacaaaaac cagcgtccaa atcatggacc tatttaaaca attcaatgat
    28501 aacggcaaaa cgattattat catcacacac gagcctgaag tagctgccct atgcaaaaag
    28561 acggtgatcc taagagatgg taatatagaa cattccgata tagagtaaag ggggaaaata
    28621 atggaagata ttattgcagc tttaagctct atattatcac acaagatgcg gtcaattttg
    28681 acaatgctag ggattattat cggtattggt gccattatcg caatcttttc aatcatcgaa
    28741 ggtaataccg aaaataccaa aagacagttg attggtggca gcaataacac tattaacatt
    28801 gtgtttaaca aaaaaagcag tattgaccct aaatttccag acaagtcaaa tgctaaaaaa
    28861 ccagactatc ttccttttat ggctgaagaa gagcttagca aaatacagca agtcaaaggc
    28921 gtcaaaaatg ccctgatctc atatggtatt gatgacaaag tctaccatct aggccaaaaa
    28981 tcatctgcaa agatttctgc catcacaaaa aacgtagctg aagtgagacg catgacattt
    29041 attaaaggca gtgacttttc agacaaagac tttattgacc aaaaacaggt gatttacctt
    29101 gaaaaaagct tgtatgagtc actatttcca aaagacgatg gccttggaaa atttgtcgaa
    29161 gtgatgggaa atcccttcag ggtcattggg gtgtttgaat caaaagaaca aagtggtctc
    29221 acaagtggga ctgagaaaat tgcttatatc ccgctacatc agtggtataa tattaatggt
    29281 gtagtggatg cgacacctga gatcacgatc cagacctatc gcgcagatga cctcaaacct
    29341 gtagcaaagc gtgttagtga tatgctcaat caaacgatcc ctaaatcaga ttatatgttt
    29401 ggtgtcatga accttaaaga atttgagcgc caactagaca acctaaacaa atctaatttt
    29461 gtgttattag ctggtattgc tagtatctct cttattgtag gtggtattgg tgtgatgaac
    29521 attatgctag tatcagttac cgaaagaacg agagaaattg ggatcaaaaa agcgcttggc
    29581 gctagacgta agcttatttt aaaacaattt ttaattgaag cggtcattct aaccttgcta
    29641 ggtggtgtga taggggttat ttcagggatg gtatcaggat tgatcatcac aagatcactc
    29701 gagtatccct atatcctatc cttgttctca gttgtcttga gtttagcgtt ttgttgtatt
    29761 atagggattg tctttggact attaccagcc attaaagcgt ctaagctaga cccaattgaa
    29821 gcattgcgat ttgaataatt aaccaaacaa gcataaaagt caaaaaggat gaccctttga
    29881 tgtcatcctt agctgcttga tacactaaac gccaatctag aaagggaagt tatgtttaaa
    29941 atattagtag ttgaagatga tgataccatc agtcaagtga tttgtgagtt tttaaaagcg
    30001 aataactatg atccagattg tgtatttgat ggccaagctg ctttagacaa gtggcagaca
    30061 acgtcttatg atctgattat tttagacatc atgctccctt ccttaagtgg cttagaagtg
    30121 ctaaaaacaa ttcgaaagac atcagatgtc cctattatta tgctaacggc attagatgac
    30181 gagtacacac agcttgtaag cttcaaccat ttgattagtg attatgtgac caaaccattt
    30241 tcaccactca ttttaatcaa acgtatcgaa aatgtattac gcgtctctac tccagatgaa
    30301 aagcgccaaa tcggagactt acttgttgat gagaccgaac atagcgtcta ttggcaagga
    30361 acattggtca aactaaccaa aaaagaatat gacattattg attatttagc aaaacgtcac
    30421 caaaaaattg tcaccagaga ccaattgatg gatgacatat ggggctattc tgaactggac
    30481 acgcgtgtcc ttgacaacca catcaagaac cttcgtaaaa aaatgacggg tattcccctc
    30541 aagacaatta caggcatggg ctatttattg ggagagcgtg agtgagacta atcaaaaaaa
    30601 cctttttggt catcaatggc ttaattattg tagtggttac ctctattttg ttagtgcttt
    30661 attttgcaat gccaatttat tacaccaaag tcaaagacaa agaagttaag cgtgaatttg
    30721 accaaacaag caagcaaatc aaaggcaaaa cggtaaccga gattagagat atcctaacaa
    30781 aaaaaatcaa caaagataat atttggtaca gcttagtaga cagtgataac caactgctct
    30841 atccttcctt gcagctgtta gacggcgtca gtgaaagcaa agacagtcaa aatgttaata
    30901 tcgtcaccac ctttgacaac tcttattcta atgttaaggt gatgagtcaa aaagtaaccc
    30961 tacgcgatgg aaaaaagatg acattgcttg ggcaatcttc acttcaaccc gtaacagatg
    31021 ctagcaaagt tctccttgac ctctacccgt cactattgat tttttcagtg accgtaggga
    31081 gtattgtcgc ctacttatat agtaggactt ctagccggcg tatccttagc atgtcacaaa
    31141 ccgccaaaaa aatggtcaac ctagaaccaa acttaacgtg taccattcac ggcaaagatg
    31201 aaatagcaat gttggctagt gatattaacc gtttgtatgc gagtctttca acgagcatca
    31261 aatccttgca aaaagagtat gaaaaagctt ctgattcaga aagagagaaa tctgagtttt
    31321 tacgcatgac atcgcacgag ctaaagacac caatcaccag tgttatcgga atgattgatg
    31381 gaatgctcta taatgttggt gactttgcag atcgtgacaa atatttgcga aaatgtcgag
    31441 acgtccttga agggcaagcc caactggttc aatccatttt atctttatct aagattgaaa
    31501 ccctagcttc tcaaaatcaa gaactgtttt cactaaaaag cagcctagaa gaagaaatgg
    31561 aagtctttct cgtcttatca gaactaaaac acctcaaagt gactatcaat ctcgaagaac
    31621 aattcgtcaa agccaataaa gtatacctac taaaggcgat taaaaatatt attgacaatg
    31681 cctttcacta taccaaatca ggcggtcaag tgatgattca actaaaagac aaccaactag
    31741 tgattaaaaa tgaagcagag acattattga cacaacagca gatgaaacag ttattccaac
    31801 cgttttatcg accagattat agtcgtaaca ggaaagacgg tggtacagga ttggggctgt
    31861 ttattaccca ccagattctt gatcagcatc atctggctta tcgctttgtt gttcttgatc
    31921 aaagatggat ggtatttacg attgattttc catcccatta tgacgattga tccacactag
    31981 taggacgaca aaagccatca atcactaacc aagccttgac ctttacccat cattgtttag
    32041 gcaagtataa aaaagaggag agaaatcata tatgaagaaa aggaaattgt tagcagtaac
    32101 actattaagt accatactct taaacagtgc agtgccatta gttgttgctg acacctcctt
    32161 gcgtaataac acatcatcca ctgatcagcc tactacagca gatactgata tggatgatga
    32221 gagtgaaaca gcaaaaaaag acaaaaaaag caaggaaaca gcgtcgcagc acgacaccca
    32281 aaaagaccat aagccatcac acactcaccc aaccccccct tcaaatgata ctaagcagac
    32341 cgatcaggca tcatctgaag ctactgacaa accaaataaa gacaaaaaca acaccaatca
    32401 atcagacagc agtgatcagt ccaccccatc tcccaaagac cagtcgtctc aaaaagagtc
    32461 acaaaacaaa gacggccgat ctaccccatc acctgatcag caaaaagatc agacacctga
    32521 taaaacacca gaaaaatcag ctgataaaac ccctgaaaaa ggaccagaaa aagcagctga
    32581 aaaaacccca gagccaaatc gtgacgctcc aaaacccatc caacctcctt tagcagcagc
    32641 agctcctgtc tttgcacctt ggagagaaag tgacaaagac ctgagcaagc taaaaccaag
    32701 cagtcgctca tcagcggctt acgtgagaca ctggacaggt gactctgcct acactcacaa
    32761 cctgttgtca cgccgttatg ggattactgc tgaacagcta gatggttttt tgaacagtct
    32821 aggtattcac tatgataaag aacgcttaaa cgggaagcgt ttattagaat gggaaaaact
    32881 aacaggacta gacgttcgag ctatcgtagc tattgcaatg gcagaaagct cactaggtac
    32941 tcagggagtt gctaaagaaa aaggagccaa tatgtttggt tatggcgcct ttgacttcaa
    33001 cccaaacaat gccaaaaaat acagcgatga ggttgctatt cgtcacatgg tagaagacac
    33061 catcattgcc aacaaaaacc aaacctttga aagacaagac ctcaaagcaa aaaaatggtc
    33121 actaggccag ttggacacct tgattgatgg tgaggtttac tttacagata caagtggcag
    33181 tgggcaaaga cgagcagata tcatgaccaa actagaccaa tggatagatg atcatggaag
    33241 cacacctgag attccagaac atctcaagat aacttccggg acacaattta gcgaagtgcc
    33301 cgtaggttat aaaagaagtc agccacaaaa cgttttgacc tacaagtcag agacctacag
    33361 ctttggccaa tgcacttggt acgcctataa tcgtgtcaaa gagctaggtt atcaagtcga
    33421 caggtacatg ggtaacggtg gcgactggca gcgcaagcca ggctttgtga ccacccataa
    33481 acctaaagtg ggctatgtcg tctcatttgc accaggccaa gcaggagcag atgcaaccta
    33541 tggtcacgtt gctgttgtag agcaaatcaa agaagatggt tctatcttaa tttcagagtc
    33601 aaatgttatg ggactaggca ccatttccta tcggacgttc acagctgagc aggctagttt
    33661 gttgacctat gtcgtagggg acaaactccc acgaccataa ccccacaacc aagaagctac
    33721 ctgcctgttg accaatctca aaaagagaca gtgtcaactt agaatgaggt tcatcagatg
    33781 ataaagaaag tgaccacccc gagtcaaaaa ataaaaaaac gtgttcgtaa tggctacctc
    33841 ctaaaactag gtacagcctg ttttctcaaa acagccaacg aaaaaagaca agttaaccag
    33901 ttcataaaaa aacatcgtag aaacgttttc aagagctaat gttggtgtaa attgactgaa
    33961 gtat
//
LOCUS       DAUCWU010000010        32532 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUCWU010000010 DAUCWU010000000
VERSION     DAUCWU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.010"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(187..600)
                     /locus_tag="VGY24_000482"
     CDS             complement(187..600)
                     /locus_tag="VGY24_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4283491.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
     gene            complement(683..1432)
                     /locus_tag="VGY24_000483"
     CDS             complement(683..1432)
                     /locus_tag="VGY24_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ4283492.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIVVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQIAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            complement(1452..3938)
                     /gene="gyrA"
                     /locus_tag="VGY24_000484"
     CDS             complement(1452..3938)
                     /gene="gyrA"
                     /locus_tag="VGY24_000484"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ4283493.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTQFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDTLAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            4129..5112
                     /locus_tag="VGY24_000485"
     CDS             4129..5112
                     /locus_tag="VGY24_000485"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ4283494.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            complement(5271..6641)
                     /locus_tag="VGY24_000486"
     CDS             complement(5271..6641)
                     /locus_tag="VGY24_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ4283495.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(6883..8610)
                     /locus_tag="VGY24_000487"
     CDS             complement(6883..8610)
                     /locus_tag="VGY24_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283496.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRDEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            complement(8613..10331)
                     /locus_tag="VGY24_000488"
     CDS             complement(8613..10331)
                     /locus_tag="VGY24_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283497.1"
                     /translation="MKRLRPYVKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLSLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KEQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPISDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            complement(10341..10940)
                     /locus_tag="VGY24_000489"
     CDS             complement(10341..10940)
                     /locus_tag="VGY24_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ4283498.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            complement(10941..11918)
                     /locus_tag="VGY24_000490"
     CDS             complement(10941..11918)
                     /locus_tag="VGY24_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ4283499.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            complement(11925..13181)
                     /locus_tag="VGY24_000491"
     CDS             complement(11925..13181)
                     /locus_tag="VGY24_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ4283500.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALVPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            complement(13171..13623)
                     /locus_tag="VGY24_000492"
     CDS             complement(13171..13623)
                     /locus_tag="VGY24_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4283501.1"
                     /translation="MLRPLSKADCPSLKEINDKALGYCVSLDLVESQFERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     KQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            complement(13641..14231)
                     /locus_tag="VGY24_000493"
     CDS             complement(13641..14231)
                     /locus_tag="VGY24_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ4283502.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKERPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            complement(14215..15054)
                     /gene="prmC"
                     /locus_tag="VGY24_000494"
     CDS             complement(14215..15054)
                     /gene="prmC"
                     /locus_tag="VGY24_000494"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ4283503.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            complement(15054..16133)
                     /gene="prfA"
                     /locus_tag="VGY24_000495"
     CDS             complement(15054..16133)
                     /gene="prfA"
                     /locus_tag="VGY24_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ4283504.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDLNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            complement(16168..16737)
                     /locus_tag="VGY24_000496"
     CDS             complement(16168..16737)
                     /locus_tag="VGY24_000496"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ4283505.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            16875..17060
                     /locus_tag="VGY24_000497"
     CDS             16875..17060
                     /locus_tag="VGY24_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ4283506.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            17113..18048
                     /locus_tag="VGY24_000498"
     CDS             17113..18048
                     /locus_tag="VGY24_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ4283507.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTVMAPSSLDCEIWTTRLFGLDKATV
                     ISCLNSLKHIEGLVVTKDQKIWMSKGLRPYFQALY"
     gene            complement(18177..19451)
                     /locus_tag="VGY24_000499"
     CDS             complement(18177..19451)
                     /locus_tag="VGY24_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ4283508.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTV
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLLLNSKDY"
     gene            complement(19451..20032)
                     /locus_tag="VGY24_000500"
     CDS             complement(19451..20032)
                     /locus_tag="VGY24_000500"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ4283509.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     regulatory      complement(20119..20214)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(20337..21320)
                     /gene="guaC"
                     /locus_tag="VGY24_000501"
     CDS             complement(20337..21320)
                     /gene="guaC"
                     /locus_tag="VGY24_000501"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ4283510.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(21598..23124)
                     /locus_tag="VGY24_000502"
     CDS             complement(21598..23124)
                     /locus_tag="VGY24_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ4283511.1"
                     /translation="MPSLFITFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKAKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(23117..23845)
                     /locus_tag="VGY24_000503"
     CDS             complement(23117..23845)
                     /locus_tag="VGY24_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283512.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPDM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            24265..24954
                     /locus_tag="VGY24_000504"
     CDS             24265..24954
                     /locus_tag="VGY24_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ4283513.1"
                     /translation="MKNAISILALLTPENQLEETFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTGF
                     YQKFDGYFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(25148..25903)
                     /locus_tag="VGY24_000505"
     CDS             complement(25148..25903)
                     /locus_tag="VGY24_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ4283514.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGAARRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(26030..27025)
                     /gene="pta"
                     /locus_tag="VGY24_000506"
     CDS             complement(26030..27025)
                     /gene="pta"
                     /locus_tag="VGY24_000506"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ4283515.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(27029..27934)
                     /locus_tag="VGY24_000507"
     CDS             complement(27029..27934)
                     /locus_tag="VGY24_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4283516.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(27931..28767)
                     /locus_tag="VGY24_000508"
     CDS             complement(27931..28767)
                     /locus_tag="VGY24_000508"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ4283517.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            complement(28742..29413)
                     /locus_tag="VGY24_000509"
     CDS             complement(28742..29413)
                     /locus_tag="VGY24_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ4283518.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            29501..30079
                     /locus_tag="VGY24_000510"
     CDS             29501..30079
                     /locus_tag="VGY24_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ4283519.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
     gene            30219..31199
                     /locus_tag="VGY24_000511"
     CDS             30219..31199
                     /locus_tag="VGY24_000511"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ4283520.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAADVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            31196..32323
                     /locus_tag="VGY24_000512"
     CDS             31196..32323
                     /locus_tag="VGY24_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4283521.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            32313..>32532
                     /locus_tag="VGY24_000513"
     CDS             32313..>32532
                     /locus_tag="VGY24_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4283522.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLAI"
BASE COUNT        10068 a         6727 c         5645 g        10092 t
ORIGIN      
        1 attttgcttt tcctatagtt attctattaa aaaacttcga caagattact ccaaaaattg
       61 ctcttcactt tgaaagttaa ccaaatcatt taacgatcaa aagagattaa tcaagcaaaa
      121 gaagatacaa caaataacta tttttaacaa gtttaatagc acgatccagt cgttaaccta
      181 ataattctat tcatgtaatt ctaaaggcaa accatccgga tcaaaaaaga aagtcatttt
      241 ctcacctgtg taatcatcat gcctgatagg ctccactgga attcctaagc tctttagatc
      301 atctacataa ctctcaatgt tggtaacacg aaatgcaagg tgcctcaaac cacaggcctc
      361 acttttaaat tctggttgac ttacacgttt aggaggggct tgatagtagg ggtcacttgt
      421 caccttacca aaaatctcca attcaatacg accacaagat aaatctaatt tgtaatcatg
      481 cttgtcagga cgataatttt ctctgataat ttcaaatccg agtttattca cataaaaatc
      541 ttttgacaga tgataatcag atacaataat agcaacgtga tgaatggcat ttagtttcat
      601 catatttctc ctttcaaaaa aagtaggatg agataaattt tctctatggt cctacttttt
      661 aaatattata tttaagatat atctaggtag atacttggtt ataagaatga ttaaaagctt
      721 tcaatacatc ggcgggcgct ttatcaaaat cgtattctgt ttttagttct cctttgacaa
      781 taatacgttc tgttgcttcg atatctgtac aagtcactaa agtcacttct ttgagaccag
      841 ctgtatcatc gataacatca acgcgttcag gagctaccgt gaaaacatct tttatgatgt
      901 attcgtaaat tttttcttta tctgttaaat agatggacat cccattttgt gctctttcaa
      961 gcggcgaaaa gagcatttga gatgaacctg taattccaaa aatatgatga ctggcaagag
     1021 aataattatt ttctcctccc ataacttgtt cttctttcat cgttcctgcg ccataaataa
     1081 gctcagtatt tcctaaacct ttaaaaattg gtaaattaat gccaagctct ggtatggcaa
     1141 tgccccctat aacaggaagt tgctgagccg ctatttgtgc ttgcaagaca ctctctgtac
     1201 taactggctc tacggcttgg aaatcaaatg ttgacttagc ctctttattc tttttgattt
     1261 gttttttcga aactttcgtt acttgatatt tattagagtt tcgagcaatt aatgtatttc
     1321 ggataggttt gttaaaaagc aatgctaaac ctagaattaa aagtacaact attagtagtt
     1381 ttcttgccca agacatggat tttatttttc gacgcttttg tttttttacc attttttact
     1441 ccttgttatg attaattatc tttatttttt gaaatactat tttcacgatc agtattgatt
     1501 tcatcgatac tactgtcttc aggctgaacc aaggtaaatg ttacaatctt agcatcagca
     1561 tctagtttca taattttaac acccaaagta gcacgacctg tttgcgaaat atttgcaaca
     1621 tttgtgcgaa taatgacacc tttatttgta atcaccatga tatcttctgt accatctacg
     1681 gtaaccaatc cagctaattg accattttta ggggtaatat tagctgtttt tattcctttt
     1741 ccccccctac cttttgtagg atagtctgta gcacttgttc tcttaccaaa gccattttcg
     1801 gtaattacaa gaacttcttg gttatcttga atacgtgacg cgccaacaac gcgatcatct
     1861 tcacgaagct taaccccgcg aacacccgta gctgatcggc ccatgttgcg gatgcttgct
     1921 tcattaaaac gaacagaata tccggaatga gttccaatga taatgtcatc ctgaccgctt
     1981 gtcaaaagga cgttaatcag ctgatctccc tctttcaact taagagcacg gagaccgttt
     2041 tgacggatat tattaaattc tgaaacttct gtacgtttga caatgccttg tttcgttgta
     2101 aagaagaagg atttacctgc tgtttcttcc ttacgtgcgt tgataatagt ttgaatggtt
     2161 tcaccatctt ctaatttcaa tagattaaca attggaagac cttttgctgt acgaccatat
     2221 tctggaattt cgtaagcttt caaccgatag acacgcccaa aattcgtaaa gaagagcaaa
     2281 gtatcatggg tacttgttga taccaattca cgtacaaagt cgtcattgtt gaccccagtt
     2341 ccttggacgc cacgtccacc acgcttctgg gcacgaaatt catcttgagc aagacgcttg
     2401 atataacctt tatttgacaa ggtaatcaaa acatcttctt cttcaattag gtcttcatct
     2461 tctagagaaa ggacttcacc gaccattaat tctgtacggc gtgggttagc gtatttacgc
     2521 ttgatctcat ccatttcttc tttgatgata gtgataatac gttctggttt ggctaaagta
     2581 tcagataagt cagcaatcaa ggccaacaaa tcatcatatt ctgattgaat cttatcacgt
     2641 tccaaaccag tcaaacgacg caaacgcata tccaaaatag cttggctttg gcgttcagac
     2701 aagtcgaaac gtgacatcaa ttctgtctga gcaatgacat ctgtctcgct gttacgaata
     2761 atagcaatca cttcgtctaa atgatcaagg gcaatcagta agccttctaa aatatgagct
     2821 ctggcttcag ctttatcttt atcaaattgg gtgcgacgta taataacttc tttttgatga
     2881 ctaatgtaat tatcaatgat ttgtctgagt gacaatattt taggcacacc attttcaata
     2941 gccaacatat taaaactgaa attcgtctga aggcttgtta atttaaagag gttatttaag
     3001 ataactgtcg cggatgcctc tcgacgaatc tcgataacaa aacgcactcc ttcacggcta
     3061 gattcgtcac gaactgctgt aataccttct agtctttttt cttgtgctaa acgaacaatg
     3121 tgttcatgca ctttcgtttt attgacgcca tagggaaatt cagtaaccac aatacgttcc
     3181 cgacctgttt gagttgtttc aatttctgtt cgagagcgta aaacgataga acctttacca
     3241 gtatcataag ccctatgaat acctgagcgc cccataacaa gggcaccagt cggaaagtca
     3301 ggtccaggga tcacttccat caactctctt gttgtacagt ctggatgttc cattaccatc
     3361 ttgacagcat caatagactc tgccaaatta tgaggaggaa tgttagttgc catcccaacg
     3421 gcaatccctg tcgctccatt gactaataaa ttaggaaagc gagctggtaa tactactggt
     3481 tctctttcgc ttccatcgta gttatcttga aaattaacgg tatttttatt gatatccctt
     3541 aataattcaa gcgcaatttt actcatgcgc gcttcagtat aacgttgagc ggcagcacca
     3601 tcaccatcca tagaaccaaa attgccatgc ccatcaacaa gcatatggcg ataactccac
     3661 cactgagcca tacgaaccat agcttcataa atagaagaat ccccatgcgg gtgatattta
     3721 cccatgacat cacccgtaat acgagcagat tttttatgcg gtttatcagg tgtcacaccg
     3781 agttcattca tcccatagag aatacgacga tgcacaggtt tcagaccatc acgcacatct
     3841 ggcaaagctc ttgccacaat gacactcatg gcatagtcaa tgaaactcgt tttcatttca
     3901 cttgtcagat tgacgtcaat taaatttcga tcttgcattt aaggaatgct ccttttctag
     3961 tcaatactag atttcattat accataaatt ccttattttt tcttattgga agcgttatca
     4021 ggaaactatt ttcattattt ttctgaattc aatgacaaac aattatcaat atgttaaaat
     4081 aagaccgtat gagcttttgc tctaataaaa ttaaggagat gtttagaaat gactgcaact
     4141 aaacaacaca aaaaagtaat cttggtcggt gatggtgccg tcggttcatc atacgctttt
     4201 gcattggtaa cacaaaatat tgctcaagaa cttggtatta ttgatatttt taaagaaaaa
     4261 actcaaggag atgcagaaga tttaagccac gctctagctt ttacatcacc taaaaaaatc
     4321 tatgcagctg actactcaga ctgtcatgat gctgatcttg ttgtcttaac agctggtgct
     4381 cctcaaaaac caggtgaaac acgccttgac cttgttgaaa aaaaccttcg tatcaataaa
     4441 gaggttgtta cacaaatcgt tgcatctggt ttcaaaggaa tctttcttgt tgctgctaac
     4501 ccagttgacg ttttgactta ctcaacatgg aaattctcag gtttccctaa agagcgtgtc
     4561 attggttcag gtacatctct agattcagcc cgtttccgtc aagctctggc tgctaaaatc
     4621 ggtgttgatg cgcgttcggt tcacgcttac atcatgggag aacacggtga ctctgaattt
     4681 gctgtttggt cacacgctaa cgttgctggt gtaggtcttt acgactggct ccaagctaat
     4741 cgtgacattg atgagcaagg cctagttgat cttttcattt ctgtacgtga tgctgcttac
     4801 tcaatcatca ataaaaaagg tgctactttc tacggtattg cagttgccct agctcgtatt
     4861 acaaaagcta ttcttgatga tgaaaatgct gttcttccgc tttctgtttt ccaagaaggc
     4921 caatacgaag gtgttgaaga ttgctatatt ggtcaaccag ctatcgttgg agcgtatgga
     4981 attgttcgtc cagtaaacat tccacttaac gatgctgaat tgcaaaaaat gcaagcctct
     5041 gcaaatcaat tgaaagctat cattgatgaa gcctttgcta aggaagaatt tgcttctgct
     5101 gctaaaaact aatcacaaaa gagacttcgg tctctttttg ttttgcctga tattcctttt
     5161 ccaacgtgct ataagagtat aatcataagt ttagaacttt tcgggttgca aaaattaaaa
     5221 agcaaaaaag ccagtgctgc tgaactgctc tggctttttc ctatcagata ctagtctttg
     5281 gcaccaagtg ctgccattgt gatgtagttg taaggtttgt tgaaatgtgg taagaagaaa
     5341 atatcagtta atgccaactt ttcaatggtc actccttctt ggatagcaag tgagaacatg
     5401 tgaattccca ttgacatatc ttcacgggct gccatttgag cacccaagat acgacgtgag
     5461 tctttatcgt aaacaatctt aatggttact gggaagttac cgtgttcgat aaattcaggt
     5521 ttttggttat cagtatactc agtcactgca gcatcaaaac caagtcgttt tgctttttca
     5581 agtgtcaaac cagttgatac catgtgcaag ccgtaaatgg aaataccgtt tgagccttgg
     5641 acaccgattc cttcaaggtc tgtaccacaa gcgttatgtg ctgctacaat tcctgtacgg
     5701 acggcatttg aagctaaagc aatgtagttt gtatcgcgag tagcattatc gtagatagtt
     5761 gcacagtcac cgatagcata aacacctgga atagaagttt cttggcgttt attgacaagg
     5821 aaagcaccat tacggaaaag atcaatctta ccatttccaa gagttgtgtt tggacggaaa
     5881 ccaacggcga ggataaccat atccacatcg tactcatttt tgtcagtaat gatcttttca
     5941 accttaccat taccagctac ttctttaact gtttcaccaa aggctagttg aataccatgt
     6001 tcttccatgt ttttagccat taagtcagtc aaatcacggt cgtaataacc tgccaagcaa
     6061 gtgtccacga catcaatcag aaccacttct ttgcctttgc gttggaaagc ttcagctaac
     6121 tcaacaccaa tgtaaccagc accaactacg gctacacgtt taatgtcttt gttttcaagc
     6181 tttgcaatca catcagctga gttttggtat aacttcacaa attgaagatt ttcaagagtt
     6241 gcttcaaatt caagtgatcc ttctttgatt tcggcacctt taatcggtgg taagataggt
     6301 tgtgagccag ttgcaaaaat caacttgtcg taagtttcca cgtggttttt accatcaaca
     6361 agcgctgtca ctgttttggc atcgtagtcg attgattgaa caggtgattc catgtaaact
     6421 ttagccccta aagactctaa ttcttcttta tctgaataga aaagtccttc tggtccagca
     6481 atttgctcac caatccaaag tgccatacca cagcctaaaa atgaaatatt tgagttttgg
     6541 tcaaatacaa caatctcatt agcatcaccg tagtttgtta acatagtctt gatacaggcc
     6601 gtaccagcat ggtttgcacc aacaacaacg attttactca tatattttgt tcctcatttc
     6661 acaatattat atagggctct attataccac agtcaaaaaa cgcttccaaa tatattgctc
     6721 acttttttga gttatttctt ctttagtcaa ctgttttttc tcacttataa aaaaaagatc
     6781 gtccgctttt tcgctgttat ttttttatat aacagtctaa agtttgttaa tgaactaact
     6841 ttaggaagag ttaaaaagtt cacctttgtt aaaagacgaa ctttattcta tcttttgtaa
     6901 tctagcataa catccgtttt ttgacattaa gctggcatgt gttccccact caaccaatcg
     6961 accttgatcc ataacaagaa tcaaatcagc tgattgaatc gttgataaac gatgggcaat
     7021 gataaagctg gtccttcctc tcatgagttc ttttagtgcc tcttgaataa cagcttctgt
     7081 tctaatatca atagaggagg tggcttcatc tagaatcaag acttttggtt ttttcaaaaa
     7141 cattctagca atagctaata actggagctg cccttgggac aaagcatcat cagaagcact
     7201 taagtaagta tcataagtct taggaagttg cataataaag aagtgtgcat gagctgcttt
     7261 ggctgctgcc actacttcat cacggctagc ctcttcactg ccgtaagcaa tcaactcatg
     7321 aacagtcgca tctttcaacc aggtttcttg caataccatg ccagtaatgg acctaagttc
     7381 ttccttagca taacacttaa taggaacttt atctaacttg atgctaccag catcaagttc
     7441 ataaaagcgc atgagtaaat tgattaaagt tgatttgcct gcacctgtag gccctacaat
     7501 cgctaccttg gaacccgcag gaacagaaaa attaatatct ttcagtaagg gcctgtcttt
     7561 ttgataagaa aaacttactt cttcaaagtc aatttgcccc ttaaccgctg atgccttaaa
     7621 tgttagaaag tgctcttgct cttttatttc aatatctaaa aggtcataaa gcctttgcgc
     7681 acaagctaaa gaactctgta tctctgccaa gacagatgaa atatcgttga aaggtttggt
     7741 atattggaca acaacattta aaaaagtggt tagctgacct actgaaaaaa gtccagccat
     7801 gatacgaaca gcccctaaac ctgccagtaa agcataaatg acactattaa taaagcgagt
     7861 ggctggattg actgttgagg cgtaaaaaat ggctttttgt gaagcctcgc aataggtttt
     7921 atttaaaaca tgataatcac agatagactg ctcttgagca ttaaacagtt gaaccaaacc
     7981 ttcttgacga aggatttctt cagtaaactg agctaaattt cctctgcttt tggtctgtgc
     8041 ttgtgcataa tgaaaactct tttgagcaat aaaacgagct aaaaagaggg agcttggtgt
     8101 taacaccaaa accaagatta acatgagcca gtcaatctgt gccatagcta taatggtaca
     8161 cagaatcgtc aatagtccaa gtataaactg gttaaaaacc atttgtagac cattgatcag
     8221 ttgctcagtg tcggttatca ctcgactaac caaatctcca atggtttgac gatctagata
     8281 tgcaaaagga agacgatgaa ttttttccaa aagcttatct ttcaattgct ggctatattg
     8341 atatagcaaa cgactataga caagaggcat cacccattga atcaatgtgt tgaccacaat
     8401 aacaactaac atttgcccca acagccattt taaagtttgc caagaattga ctgacaaact
     8461 catatcaacg gctttaccga taagaacagg caagtaaaca cttaaaccaa cttgtacaaa
     8521 actagcgatc acgagaatac aaacaggtag aggttttttt aacaaatcct gaagcactcg
     8581 ctttaggagg tgatggtctg ttttaatcat ttttactccc ccttcacgta aagcgctagc
     8641 atttcttgat aaagtgcatt gcttactttt aactggtcat gactggcaaa atccaattga
     8701 tggccctgtt ccaacactaa aatcttatca gcaaactgca aattcttaat ggattgcgtg
     8761 actagaatca ctaaaacatc tgataactct tcttttagag ctttaaaaag tcgtcctcga
     8821 gtctcattgt ctaaggcgga actggcatca tccaaaatca aaatgggttt tggcttcaga
     8881 agtgctctgg caatagctag acgttgccgt tgccctcctg aaaaatgacg gccgaaggct
     8941 tcaacaggtg cctctaattg ttctggtaag gcagcgacaa actcttttgc ctgagccaat
     9001 tcaagagccc gccacagttc ctcatcacta ataggcattg attgtcccag taggaggtta
     9061 cttctaatcg ttcctttaaa taattgagct ttttgaggaa caagagcaat cacatttcgc
     9121 cactcggtta gtgtttttgg cacttctcct tgccaattaa gactaatctc accactatat
     9181 tgtgaataag tttggcagat caagtcaatc aaagtcgttt tgcctgctcc agtaccacca
     9241 atgatgccaa tccattctcc ctgatcagca cttaactgaa tatcatacag acttggctct
     9301 gctgctcctg gataagtaaa ggtcaagtgc cggatggtca acagatgagt tgaaatagga
     9361 ccatctttaa gtaagggcaa aggcgcttca tcagctaaaa caaacacctg attaatccgt
     9421 tttgcggctg ttacactttg attgatggtt cccatcaaca tggtcatttt caaaagttct
     9481 gccaaaatct gcaagaggta attaattaaa gcaaccaaca tgccctgact aagcgatcta
     9541 tgagctactt gccaacttcc ttgccaaatc aagattaaca gagtaagatt gaccactaaa
     9601 aaagtcatag gattcaagac attggctagg gtagcagctt gatattggtg gcgacttaag
     9661 agcatatttt gctccttaaa tgcttggagt tctttttggg tttgattaaa agccttaatt
     9721 actcggattc cttgtaactg ttggctagtc aaacgaacca agtgatctgt ttctcggcgg
     9781 attaagagat aaaggggacc taataaatgt gacataacaa aaacaagtgt tagtaagaca
     9841 atcaccatca tcacaaacca taggcttaga cttggactaa tccaataagc cattaccata
     9901 gcaccacata cgataatagg ggcccttaga aaaagacgca aaaattgatt gataccagtt
     9961 tgaatttgaa aactatcact ggtcaatcgt gataacagac tagcataacc aagatggtct
    10021 tggtcctcct tgctcaaaaa catgattttt ttaaacaaat catctgtcat ttgtcttgta
    10081 aaaccaacag ctgctttcga agaaaaatac tgggctgtaa cggaaagcaa taagccaatg
    10141 gttgctaaac caaataatgt taaaacaacc ctcaaaattc cctggctgtt gtgttgacta
    10201 atcgatatat caatcatgtt agcaattaac aaagggacta ataattcaaa taaagcttct
    10261 aataatttaa aaagaggacc taagatactt tcttttaggt accctttcac atatggacgt
    10321 aatcgtttca tgataacttt ctaaaaaagg ctcatcagtt caattaaata aagattgaaa
    10381 tgaacttctc gtgagtaata gatattgccg ccattcttat aaagcttacc tccagaaatc
    10441 aaggcgaggg ggtggtaata aaaataggtt tgaccactag tgttgcctag gcttggcgcc
    10501 acaacggttt tggaataggt agtcgctaaa tcaacggtat tttgacctcc atgtttagca
    10561 atataatcaa tataagctgt tccaaaatta taggcctgaa cagccgtcca agaatctact
    10621 cctgcttcct cagctagagc aagattatga gacagtaaat tcaccccgtg ctcaatactg
    10681 gcctgactat ctgtaatcga gtttttttgt cctgaactgc tttcactgga ttgcatgaca
    10741 tctgcttctc ctccttttgt ctcggtgtat atcatagcta agactaagtc tacatttgcc
    10801 tttgtatcat tttctgctaa tgttttctca accataggct tatatgccaa aacacgttga
    10861 acattgtgat gaataacaaa ggattgataa atgacaaaaa gaagaagaaa actgcaggca
    10921 cgctttaaaa gtctaaacat ctatttggtt ttaatatcct ctatattttt aatcaagatt
    10981 gaataagtcc catcatcatt taacaaaaat tcaaccgctt cagcatcctg ataaatggca
    11041 tttggtactg ttaattctat cccgtttgat aacgatagct tttgattttc tagttttttt
    11101 atctgtctag aatgatcaat atcgctaatt tttataggtt ctggaatgac ttctttgact
    11161 tgatccacaa aggttaaacg ggctgttaaa ttatcatcaa agagttggtc agctaatttt
    11221 tctggtgata aaacgtcgtc tgcttctaac tgcttaaaaa gagtggattt catctttgac
    11281 tgaaaagtaa aatcatcttg attaaaatgc tctgcaattt tttgagcggt ttgttctatc
    11341 attttgatag actttttaac agattgctca ggtgtaactt tcaagaggtg ttctgaaaaa
    11401 taattagcaa aactgccatt atgcttcaca cgcttttcaa tcaagtagta ctgaccagat
    11461 ttgagattaa tgactagtgc ttcatctggt gtctgggtgg gactcggcag attattctgc
    11521 gtcaccgtaa atggatgttc ataattgtca gaaaggtgag caaactgttc tttcaaagca
    11581 attcgcaaga aagcaaaaaa gggttcacca tccttatcaa actggacaaa gactaaatca
    11641 ttggtttttt gatcctctga aataacaaag gcttccttcc ataactgggc aatggtaaca
    11701 gacgtttcca acaaatcatc accaatggtt gtaaaaaagg tattattagc ctcaaactga
    11761 ccacgtttgg cttcgtccga gaacactttg gccaattttt tccggaaata ttcatcaatt
    11821 cgcggtgtaa tgctcactaa acgatctgat aacagtaact cagtgtcatt gggagaaaat
    11881 tgatgaatga caatacgttt tatataacta tctaacatga ttccttactt tttatagagt
    11941 gggaaagcat cggttaactg acgcacttcc tgacggactt cttcaagaat agtttcttga
    12001 tcatgattaa ccaaggcttt gatgatgagg tgagcgatag tttggctttc tttaactccc
    12061 atgccacgac tggttatggc agcacaacca atacgaatgc ctgaggtttt aaatggcgac
    12121 aaggtttcaa aaggaatggc atttttatta agggtaatat taacctcatc cagaagattt
    12181 tgcgctagtt taccattagc aataacctta gtgacatcta ctaaaaagac atgattatct
    12241 gtaccacctg agatcagacg aaaacggtca tcttgtgcaa aaactgctgc catagccgca
    12301 gtattatcaa taatagcttg tgcatagtct ttgaaggctg gaaccaaggc ttctttgaag
    12361 gcaaccgctt tggcagcaat cacgtgttcc agaggtcctc cttgtaaacc agggaaaacg
    12421 gccgaattaa tctttttagc aagagcttcg tcattggtta atatcaagcc tccacgagga
    12481 ccacgtaagg ttttatgggt tgttgatgtc acaatatgcg cataagggac aggactcggg
    12541 tgcacacctg ctgctacaag gccagcgatg tgagccatgt ccaccattaa gtaagcacct
    12601 acgtgatcag caatggcacg aaacttttca aaatcaatgc ttctagagta ggccgaagcc
    12661 cctgcaacga taagctttgg ctggaccgcc tttgcttgtt ctaaaatcgc ctcataattt
    12721 aacatttcag tatcagcatc tactgagtaa cccacaaaat ggtaggtttt tccagaaaaa
    12781 tttactggag agccatgcgt gagatgacca cctgcagcca aatccattcc taatacggtg
    12841 tcaccagcct caatcaaggc catataagct gctgcattgg cttgacttcc ggaatgggct
    12901 tgaacattgg caaatgcggc accgaaaagt ttcttagcac gttcaatagc aagcgtttcg
    12961 acaatatcca cacattctgt tccaccataa tagcgattac ctggatagcc ttctgcatac
    13021 ttattggtta aaacagaacc ttgagctgcc atgacagctt ttgaaaccat attttcagat
    13081 gcaatcaatt cgatatgatg ttcttgtctt tcttcttcag catgaatggc atcccaaagc
    13141 tctttatcaa agtcttcaac attaccttta tcaaaaatca ttgtcatctc cttgtaagtt
    13201 tttgatgaat cgtaattgtg ttttatcatc tgcatagtcg aggtttcggt aaaaagcatg
    13261 cgcttcttta cgatgactgg cagaattgag gcgaataaag gcaatgcctt cctgcttggc
    13321 ttgggattcc agggccctta gtaaggcact cccaattccc cttctctgat aggctggtaa
    13381 aacagccaag cccaagagat taagaccatc agaagcataa agggtttcat accgttcagc
    13441 gtgaacatag cctaataatt gattggtatc cttgtcggca taagctaaaa aatagtgatg
    13501 gcaatcctca atcaatcgtt cgaattgact ttccactaaa tcgagtgaga cacaataccc
    13561 taaggcttta tcattaatct cctttagtga tggacaatct gctttactta atggtctcaa
    13621 cattccaagc ggctcctctc ttaaaagcga agttcaggca cgttggctaa taattcttct
    13681 ttggtgatag caccttgacg taaaatcaca gcacgctcac ctgataagtc taagatggtt
    13741 gaatccttac ctgttagaaa aggatcatct gcataaccaa atacttgaaa gtcaaaatct
    13801 tgcataatat gatcaaaaac tcgaccacta gctttgccag ataaatttgc agaaggacca
    13861 attaaagggc ctgttttttg aattaaagct gcggtaattg gatgactggg taagcgaaaa
    13921 ccaaccgttg aaagacccga attaatccaa taaggaacct ggtcattagc ttttaaaata
    13981 atcgttaacg gacctggtaa aaaggcttgg tataatttct tgaggtatct cggccgttct
    14041 tttgaaaagg ctaagatact attaaaatca gccacattaa ggttcattgc tttatctcgg
    14101 ggtctttgtt ttaagtcata aacagcattt actgcttttt catctaaagc cttggcaaaa
    14161 agaccataaa ccgtttctgt tggcaaaaca agggcatcac ctgattcaat gatactagct
    14221 agatactcca taatcaacaa ccaccatcct ttctttgcca aatatatccg ttacgactcg
    14281 tatatgtctt tgaggaaaat aggcttgtaa catatctttt ataccttcag cttgcttata
    14341 accaatttca aagtaaagct tcccctcctt agttaagtaa ttgtcagctt gttcaataat
    14401 cttacggtaa atagcatagc cattttcttt agcaaaaagt gccaaatgag gctctgattg
    14461 caaaacattt agactgactt cttccttatc ctcgtaggat atgtagggag gatttgacac
    14521 aataatatca aatgttcctg aaatgagact aaaaacatct gattcaataa acgttatatc
    14581 aagctggtaa gcatcagcat tggcttttgc caagtcaaga gcagctctag aaatatcaga
    14641 tgccgttact tgccagttag gcctttcttt tttgagagaa atagcaatcg ctccactacc
    14701 tgtaccaata tctaaaacat tcaacggtgc atcaagattt tcagctaaaa tcatgtcaac
    14761 taattcttct gtttctggcc ttggaattaa tacacgcttg tcgactgcaa gcttcaaatc
    14821 acgaaaataa gcattacctg taatatattg aggagataaa tgctgactta gagaacagaa
    14881 aatatgttcc aaaagaacag catcttcagg agtcacagct tgattttggt gaatcaacat
    14941 atctagactt gaccattctt tgatttctct aaaaacatag gcgagatttt cgcgatcctc
    15001 atctatttgt tctagtttat cttcataagt tctgattaat gtagcatagt tcattagtta
    15061 ttgagagatt ctaatttctt tgtctgatca tacataacga gtgcatcaat aacttcatcc
    15121 atttttccag ataaaatagt atctaatttt tgaagtgtca aaccaatacg atgatctgtt
    15181 acacgatttt gtgggaaatt ataagtacga atacgttctg aacgatcacc agtaccaact
    15241 gttgatttac gttcagcatc ttgttcatct tgagcaattt gcgcaaaatg gtcagcaaca
    15301 cgagcacgaa tgattttcat cgctttatca cggttttttt gctgagtacg ctcttcttgc
    15361 atttcaactt tgattcctgt aggaatgtgt accatacgaa ctgcagtagc aactttattg
    15421 acgttctgac caccagcacc agacgcgtgg taaatatcga cgcgaaggtc tttaggatca
    15481 atgtcgtatt ctacttcttc aacttctggc attaccaaaa cagttgcagt tgaagtatga
    15541 acacgaccct gactctctgt cactgggaca cgttgaacac gatgagctcc tgattcatat
    15601 ttaagcttag aataaactga ctgaccagaa accatggcaa ccacttcttt aataccacca
    15661 acaccattaa cggatgattc cataacttca aaacgccatc cttgagtttc agcatatttt
    15721 tgatacattg ttaaaagatc accagcaaaa agcgctgctt catcgccacc agcagcacca
    15781 cgaatttcta aaataatgtt tttatcatcg ttaagatctt taggtaatag aaggattttt
    15841 aatttttctt catactcttc tttagctgct tttgattctt taagttcttc tttagccatt
    15901 tcttctaatt caggatcacc actggcatct ttgatcattt cttcagcatc tgaaatggtt
    15961 tgaatgactt gcttatattc tctataagct gtgacagttt ctcgagtatt agcttcttca
    16021 cgtgataatt ccataaatcg ctttgtatct gaaacaacat ctggatcact aagtaactca
    16081 cctaactctt cataacgatc ttcaactgcc tgcaattgat cataaatatt catttttctt
    16141 tgtatctcct ttaaaaccga ttacctttta aatatctgga ttaaaataat gttttcgaca
    16201 aactgggata tatgtttcat taccaccaat ttgtatctga tcaccttcat agacaggttt
    16261 tccgttttct gtccttaaaa ccatggttgc tttcttacta cagtattggc aaattgtttt
    16321 gatttcatca atcttatcgg ccaataatag caaatgtttt gaaccttcaa aaagattgtt
    16381 ttggaaatca tttttcaaac caaaagccat gacaggaaca tttagttcat ccaccacacg
    16441 cgccaaatca taaacatttt gcttgcttaa aaattggctt tcgtcaatca ggacacaata
    16501 aggtttttcc tccaattggg caataaatgt gaaaatatcc atgtcatttg aaatggggat
    16561 agcttctcgt cgcataccaa tacggcttga gacgatgcca aaaccatcac gtgtatcaag
    16621 agcacttgtc ataatgacaa caggtttgcc ttgttcttca tagttatgag caacttttaa
    16681 aatttcaatg gttttaccag aattcattgt cccatattta taatagagtt gagccaaagc
    16741 ggaaaatcct actttctata gtattctacc tattttacca caaaggctga agttttccca
    16801 gccattgaaa acagtgtata ctttttcaca gcattatgtt acaatgatgc ccaataacat
    16861 aaaggagtaa cattatgcca tttgtaacaa ttgacttgtt tgagggacgc tcacaagaac
    16921 aaaaaaatca attagctcgc gaggttaccg aagtagtctc acgcattgca aaagcaccta
    16981 aagaaaatat ccatgttttt atcaacgata tgcctgaagg aacttactat cctcaaggtg
    17041 aaatgaaaca aaaatcataa tgaccttccc ccactcctta gtgggggtat tctgctaaaa
    17101 agaggactaa ttatgtttgt tacgcaacaa ttaaagctaa tgggaacagt tattgatatt
    17161 cagatcgaat ctgacaaagc ttgccaacaa ctaagtagag tcattgactt attatatact
    17221 tacaaaaatc gtttttcagc taatgattca aactctgaat tgatggctat taatcaggcg
    17281 gctggtgtta aacccgtcag cgtccactct gatttattta acctaatcca aattggtaaa
    17341 gctcatagct tgtcaacacc aagtaatctt aatatcgcca ttgggccact tgttcaagcc
    17401 tggcgaattg gatttgaaga tgctagagtg cctagtcata accttatctc acaacagtta
    17461 gctctaacag atcctagaca ggttcttata gatgacaaaa aacagactgt ctttttacaa
    17521 caagtaggaa tggctttaga cttaggagcc ttagcaaaag gttatattac tgataagatt
    17581 atggcctacc tgattgaaga tggtattgac tctgccctta ttaatttagg tggaaatgta
    17641 cgcgttcatg gacctaatcc gaaaagtcct gacaagactt ttcgaattgg cattcaaaaa
    17701 ccagatgcta aacgtgggca acatcttggt gtcatcaagg ttaacaatca ctctgttgtg
    17761 acctctggta tttatgaaag gcagtttaca gccaaaggga aacagtatca tcatatctta
    17821 gatcgtcaga caggctatcc tatagaaaca gatatgctca gcttaacggt catggctcct
    17881 tcttctttgg attgtgaaat atggactacc cgactctttg gactcgataa agccactgtt
    17941 attagctgtt taaatagtct aaaacacatt gaaggacttg tggtcacaaa ggatcaaaaa
    18001 atctggatgt caaagggact aagaccatac ttccaagcct tgtattagta aaaacaacta
    18061 ggagtctttc tagctaactg attaaaccta tcaaaaaaac gactctctct gagtcgtttc
    18121 aacttgagga caaacttcat ttttgaggtt tgtcttttag tatgatcact gatatcttaa
    18181 taatctttac tatttaagag caggtttaag acaacagatg tcaaagtggc aatcacaata
    18241 ccattcgtta agaacatttg agctgtctct ggtaagctgg caaaaagatt agtgccatta
    18301 aagcctaaac ctgctgagat ggaaacggca gcaataataa aattatactc gttcttttgg
    18361 aaatcaacac gattaagcat ttgcattcct tgaagagcaa ccataccaaa gagaacaagc
    18421 atggctccac caagaactgg gcttggaatc atttgtgcca ttgctccaaa tttagggagt
    18481 agaccaatga ccacaaggat acctgctgcg tagtaaatcg gacgacgggt tttaatacct
    18541 gagatttgga caagtccaac gttttgagaa aaaccagtgt aagggaaggt attaaataac
    18601 ccaccgagaa agactgcgat cccttctgag cgataaccat tgcgcaaacg tttttcatct
    18661 aattgatcat tagtcaaatc tgaaagcgct aagtagacac ctgttgattc aaccatagaa
    18721 accgttgcga tgatacacat catcacaata gaagtgattt caaaagttgg catgccaaaa
    18781 taaaatgggg ttggcacatg aatccaagaa gcctctacaa caggagttgt atctactaat
    18841 cccatcatgg ctgaaaccaa agttcccgct acaagcccaa tcaaaatgga aatggatttc
    18901 acaaaacctt tggtaaattt ttggactaaa agaataatga caatcgtcaa caatgataac
    18961 atcatgcttt gtaccgtagg ctctttgacg ttatctccca tattccccat agcaacacct
    19021 acgagactta agccaataac ggtaatgacc gatcctgtaa caattggtgg gaagaaacga
    19081 gcaatcttag aaaaaatacc tgccactaaa atgacataaa ttcctgacgc aatgagggct
    19141 ccaaacatgg cacctgaacc ttgttgcgct ccaataatgg atagaggtgc cactgattga
    19201 aaagcacatc ctaaaacaac tggtaatccc actcccgtat gcttggttaa ctttaactgt
    19261 aagaaagtag ccacaccaca catgaaaata tctgtcgaaa tcaggtaagt caactctcta
    19321 gcggaataac ctaaagcacc tgcgatcata attggtacta aaatagatcc ggcatacatc
    19381 gaaagcacat gctgtaagcc aaggacagct gattgcgagt gagaatgttc ctgctttgtt
    19441 gaatgtgtca ttatgcgtca gcctccaaga agtttagatt accattttca aaatttttaa
    19501 tacgtgctaa tgaggtcact tcaatgccca tatcttcaat caatcgacga ccatcttgga
    19561 aagatttctc aatcacaata ccaacgccga cgacttgtgc ccctgcttga ccaataatct
    19621 cgatcaagcc tttggctgcc tgaccattag ctaaaaagtc atcaataatc aaaaccttgt
    19681 cttctttaga taggaattta ccagcgatag acaccgtgct cgtcacttgt ttagtgaaag
    19741 aataaacttc tgctgtcaaa atgccttctg tcatggtaat gtttttatgt tttttcgcaa
    19801 aaatcatagg aacatccatt gcttctgcag cgtatacggc tggtgcaata cctgaagctt
    19861 cgattgtaac cacttttgta atgccagcct cagcatattt ttgagcaaac actttaccaa
    19921 ttgctttcat caaccggtaa tcaacttggt gagttaaaaa attatctacc tttagaatat
    19981 tctctcctag aatattgcca tctgttaaga tgcgttcttc aagtaactgc ataaatctcc
    20041 ttttgattca aatagaaaaa gcctgccgca aagtgagaaa ctcttatagt ttctcacttt
    20101 gcaaggttaa aaaccaagca agcctaaaag cttacttatt gttagacatt aaggtgtctt
    20161 gtagagacgc tgcgccaatt cacagcataa ataagtatgt cattattgaa ttcattatga
    20221 taatttacta ttttaccatg tttcttattt ttttcaatac ctacagagat atgataaaag
    20281 catctccagc attaagctaa aaaattcttt actggtccta gctaactcca tatcatttaa
    20341 atggaatcac cattccaaat ggaatttttg acaataacat aatcaacatg acgcaaactg
    20401 tctaaatctt taccacccgc gtaagaaatg gacgattgca aatcctgttg catttcggtc
    20461 agggtatctg ataagtgacc ttttgtaggc aataagattt ttttgccttc tacattttta
    20521 tgctcgcctt tttgatattc tgacgccgag ccataatatt ctttaaacgt ttcaccatcc
    20581 acttctaccg tttttccagg actttcaata tgtcctgcaa aaagcgatcc aatcatgacc
    20641 atactagctc caaagcggat ggatttagca atatcaccat gtgtgcgaat cccaccatca
    20701 gcaatgatag gtttacgagc tgcctttgcg caccagcgca gtgctgctag ctgccaacca
    20761 cctgtaccga aaccggtttt aactttagtg atacatactt tgcctggacc gatgcctact
    20821 ttagtagcat cagcacctgc attttcgagt tctcgtacag cttctggcgt accaacattt
    20881 ccagcaatga caaatgtttc tggtaattca gttttgatat gcttaatcat atcaataaca
    20941 ctgttagcat gaccatgagc aatatcaatg gtgataaact caggagcatc ctctttcaaa
    21001 gaggtcacaa attcatattc acaagcttta accccaacag agattgatgc aatcaagcct
    21061 tgttcatgca tgcgtttgat aaaaggtttg cggctatctt catcaaaacg atgcataata
    21121 taaaaataac cctcttttgc caactgctca gcaattgttt catcaataat ggtttgcata
    21181 tttgctggga taactggtag cttgaactgg tattttccga gtgtcacact tgtatctgct
    21241 tgtgaacgac tagtaataat gcacttgtta ggaatcagtt gaatatcttc ataatcaaat
    21301 acagggatgt cattaaacat aagtttctcc tcttttaaca tagattaaga aaaacaagtc
    21361 agttctcacc ttcttctcta agtcctctat acaaaataat cttcctatga cctctgatga
    21421 ttagaggtac ctttaggtaa aaacttacct ttcattttat agattactta tttgtttata
    21481 ttttgtcaaa tttatatgct gtttttaatt ttaaagttcg gtttttgtat ttatgaattg
    21541 ttacttcttg aacttaagag acgaaaaaat gccagtccta cggaccaaca tttctgttta
    21601 tttctggatt aagcctgttt tcaacagata atctcgagcg accttattag catctgctcc
    21661 tttaacagat acctcgtagt tcatgtcctg catctctttt tcagtaattt ttcccgctaa
    21721 ttgattaaga atgcctttta attctggatg ttttgttaat aaggaagttt tcatcaaagg
    21781 agctccctga taaggtggaa acaactgttt atcatccttt aaaactttca aatggtattt
    21841 cgtaatttca gcatccgtag agtaagcatc tgttacttgg atatctcctg attgaattgc
    21901 ttgataacga agagctggct ccatagtcgc cacagatagc tgtaatccat attgagattg
    21961 cattccctta tagccatctg ctctgtcctt aaattccaga gtaaaaccag cctttaaagt
    22021 atcagcatgc gcttttaaat cagaaatggt ttcaatctga tattccttag ccaatttttc
    22081 tggcatagca acagcatacg tattttggta agcaaatggc ttgagaaggg tcaatttatc
    22141 ttgcttagca atgccttttt tagcatcctc atagacctgc ttagggtcat tagacaaagg
    22201 tggtttgtcg cgtaaaagac ttgatgttat ggttcctgta aattcaggat agatatcaat
    22261 atctccagat ttaagggctt catataagaa gctagttttc ccaaaattag atttcaactc
    22321 aacacttata tctgattggt cttcaattaa ttccttatag agattaatca gaatatcggg
    22381 ctctgctccc aatttaccgg caataaccac tgtatctttt gcttttgaaa actgactaag
    22441 cgccatagga gtatacgatg ctagcaatgc aagtaaggtg attccaaaac taatcataat
    22501 ccgtcgcaag gaagctttct caaggtactg taacaaacta ttgaaaataa tagctagcag
    22561 agctgaagaa atagccccta tcagaatcag gttagcatta ttacgatcaa tccctaataa
    22621 gatgaaagag ccaagtccac cggctcctat caaggaagct aaagtagctg tgccgataat
    22681 catgactgct gacgtccgca cacctgacat aataacaggc attgcaattg gaatctcaaa
    22741 cgtttttaac cgctcccatt tggtcatccc aaaagctatt cctgcttcca cgagacttgg
    22801 gtcaatacca tttaatcctg tgatggtgtt ttgtaaaatc ggaaaaatcg catagataac
    22861 taaagctgtc actgcaggca acgttccaat tcccattaaa gggatgaaaa gaccaagcaa
    22921 agccagtgag ggaatggttt gaaaaacacc tgttacctgt aacataatgt ctgaccaacg
    22981 tttgctgcga ctgagaaggg ctgctaaagg cacgcctatt agcaaggcga tcataagaga
    23041 taaaagggaa atttgcaggt gttcccccag agccgctaac cattcattaa aacggttttg
    23101 aaaggtgata aacaaactag gcatgctcat cacctccaaa taaattggcc acaaaagcat
    23161 tagcagggtg ggtcttaatc atttctgggc gatccagttg cacaatttct ccctcattta
    23221 aaatagccac gcggtctcct aatttaatgg cctcatcaat atcatgtgtc acaaatacaa
    23281 tcgtcatgtc aaactcctta tgaagactca acattaactc ttgcaattgt ttacgtgaaa
    23341 taggatctaa ggctgaaaat ggttcatcca tcaatagaat tttaggatga gaaatgatag
    23401 cacgtacaat accaatacgt tgctgttcac ctcccgataa atcactcggg taacgatcaa
    23461 gataatcttt agcaggtagg cccaccttat ccaataattc ttcagttttt tgtctaattt
    23521 cttcagctga ccattgcttc atatcaggga taatcgcaat attttcagct actgtgagat
    23581 tggggaaaag agcaatttgc tgcaacacgt atccaataga tagcctcatc tcacgtaaat
    23641 ctaactcttt ctgtgggacg ttattcagta aaatatctcc agagctgggt tcaataaggc
    23701 agttaatcat ttttaaaagt gtggttttcc ccgatccgct ggggccaacc agcacaaaaa
    23761 attcacggtc attaatttga aaggtctgct cttgcaaaac ctttgtctga ccaaaagttt
    23821 tagaaacatt attaaaacgt atcattattt ctccaattca ttggttaaag taacagcata
    23881 aaagctgatg agttcattca acctgtctct ctgtcatttt ttctcaaaac gttctgagta
    23941 actacaagga atcactgcat gccctaccat ttttagacaa acgcatctac taattaaagg
    24001 gactcttagg tatcctcagt aaggtaaaaa ctaccaattc tcaaaaacat tttctctcat
    24061 tattttcttg tcgtttgttg ttgaccatta taaaaagttt cccactataa gtcaaataat
    24121 gagactatta cccccttata gaaaaacagg tcaccgggtc agcctatctc tccaaaatta
    24181 ccttatcaaa aatcctagac ttgcctatga tgatagataa gcagattaat tgtcttttct
    24241 agaaaataca gctatcatac aattatgaaa aatgccattt ccattttagc gctcttaaca
    24301 cctgaaaacc aacttgaaga gacgtttatt agagagcttt cccaccactt ctcacacctt
    24361 tttgaggtga ctattctcac tagcaaagcc aacattcagt caaatcaatt gtcgactttc
    24421 caaggaattt tcagtttcca tgagcatgac attgacttgc ctacactcta tttcaaaact
    24481 tctcaacatg gtcagggctt tttagtgaca gaaagtgtct ttgatcaagc aacagcagtc
    24541 ttatcgttaa gccaatacct cactggattt taccaaaaat ttgatggata ttttttacag
    24601 taccttcctt tgcaagcaag gttatctgat gcaaatggaa acatcatagt cgataatcat
    24661 gcttttaatg gaagtttttt gccaactact gataaagaaa tagaagattg gattcttgct
    24721 gaattgcgtt tatcagataa cccttgtaaa accttcctac ttccttcggg ttcattggat
    24781 catatttaca tgcagcatta tcaagctctt aaaaatcctc aaggtcagct ggtgggagtt
    24841 ttggataccg ttcaagacat taaaccgtta ttaaaccagt atttagaaga aactggacaa
    24901 gccattgttg gctggtccga tgtcacatca ggcccttcca ttttagatca ctaaacttga
    24961 ttttaaatag ggagttcagc ctaaatcatc aacgcacaag aagcaatcat gaacgcttga
    25021 agacctcaaa accttcttct tgattgcttt ttttgatgac taaatcggta agtctcactt
    25081 ataaagaata atccatacca gtaaaccatt tttctaaaga ttattattct tatggactca
    25141 tcccttgcta gtcacgacta actggttgcg gtccataact ttgcgacact ggcatcagct
    25201 caatacgatt aacattaaca tgagggggct gatgaatcac ccatgataca gtcgacgcaa
    25261 tatcttgagg ttgaatggca tgtgctcctt gataaatggc cttcacccgt gcctcatcac
    25321 cattaaagcg aacctttgaa aattctgtcc cttcacatag accgggttca atattggtca
    25381 cacgtatatg gctacctgct aaatcagctc gtaaattgag tgagaactgc ttaacaaaag
    25441 ccttgctagc cccataaata ttagctcccg gatagggaat tgtccctgcg gttgacccta
    25501 aattaataat aataccatca tctttggaga ccatatgtgg taataattga cgtgtgagat
    25561 aaatcaaccc aacaatatta gtgttaatca tcgtcatcca attctcaaaa tcggcttcat
    25621 aagccttatc aagtcccaat gccaagcctg cattattcac taaaatagta atatcttgcc
    25681 attgatttgg tagacttgtc aaagcatcat caatcgcttt ggtatctgtg acatccatct
    25741 gcaaagggta aaagttgtcc gacccaaagc tcgtttctaa ggccttgagt ttctcaagac
    25801 gacgagcggc accaataacc tttatcccat ctgcaattaa gtgttcgaca atggctctgc
    25861 caaaaccggc tgaagcccct gttactaagg caatatttcc catacatttc ctcaccttca
    25921 aaaaaaacga gatggtctgg ccaactcgtt tttaataatg tcaattagtc ttaggacaac
    25981 ttctaacaca tcacttaacg gttagaatcc tatatcaagg ggtcttgagt tacccttggc
    26041 tttcgatagc ttgtgctgct gtaataatag ctaatttgta aatatcttca gcgcttgagc
    26101 cacgagataa atcattaaca ggtttgttca atccttgtaa gattggtcca atagcatcaa
    26161 acattcctaa acgttgtgca attttgtaac caatatttcc agattgtaag tcagggaaaa
    26221 taaaagtatt ggcttggcca gcaactgctg aatctggtgc tttgatagct gccgtttcag
    26281 gaacaaaagc tgcatcaaac tgcaattcac catcaagagc taagtcagga ttcaaccctt
    26341 tagcaatttc tgttgcttct cttaccttgt caacttgagg cgctttacca gaaccttttg
    26401 ttgagaagct taacatagca attttaggat caatatcaaa gattttagct gtttcagctg
    26461 tgttaacagc gatttctgcc aattcttgtg ctgttgggtc aatattgatg gcacaatcag
    26521 caaacacgta acgctcactc gtattttcgc gattcatcaa gaaaacacct gaagttcttg
    26581 aaatacctgg tttggtttta atgatttgaa gggctggacg aacagtatca gctgttgaat
    26641 gaatagcacc agataccatt ccatctgcta atcccatttt aaccaacata acaccaaagt
    26701 aattcacatc acgaagcatt ttatcagcat cttcaagcgt tgcttttcct ttacggatgt
    26761 caacaaaagc ttctttcatt ttgtcaaaat ccgcatattc atttggatta atgatagtat
    26821 agtcttgatc agcgaaacct aattttgtca aaaggttgcg tacctcttct gactgtccaa
    26881 gaataatcgg ttctaagagt ccttcaaatt tcaaacgagc agctgcacgg acgacgcgct
    26941 catcattacc ttctgggaaa acaatcttca tgtttttacc aagaattttt tctcttaaac
    27001 caccgaataa actacgaata ctcattcttt aataacctct atcttttttc tgtaaaccta
    27061 tgataacgct atcaaagtca tctgtcaagt gtattgcata gctacaatca ctttctgtaa
    27121 atggatcttt gaaattcaaa taatgacaat gtaaagcttg ccttgttatt cctaagtcca
    27181 aacgacctcc gtaaaggtca tcccctaaaa gtggaaaacc aatatgggca aaatgaactc
    27241 tgatttgatg ggtacgtcct gtatgcaatt tgatatccac taaatgcaca ttttctgaat
    27301 agcgcgccac aaccttatag ctagtcttgg catatttgcc catagggtca actgccctgg
    27361 taataatact atcttttgaa cgcccaattg gagcaataat atctccttca tctggcaacg
    27421 tcccatttcc agaaaccaga gcaaaataac gtttctctat tgagcgagtt tggagttgct
    27481 tgtccaacct tgcatgagca taaccatgct tagcaaataa catcaagcca ctcgtatctc
    27541 tgtctaaacg agtcacgata tgcacttgtt ggtctaggta atgattgtcc acataatatg
    27601 ccttgataaa gttagcaatg gtattggagt ggatggcact tggaatactg gcaaagccaa
    27661 tgggtttatt aatcaccaaa aaatgatcat cctcgtgaac aatatccaaa tcaaaaggga
    27721 tggcttcaag cttttcaaag ggctcttcgt ttggaatgtc aatggtgaca acatctccca
    27781 cttgcaataa ataaatggca ttttgttcaa taccattcac taatatatta ccacccttat
    27841 atttgatttt agccaatagt cctttagaga catcatgact ttttaaaagg gtttttactt
    27901 ttattcgttt gtctgctaca aattcaaatc tcatgagtca atctctccaa taaaggcatc
    27961 cttgaccctt tcccaaaagc tcgtatgact cggagaggaa acaaaatgaa ttttctcatc
    28021 gtcgataaaa tactccacct tcgtcacatt ttttaactga taggttttat tatcaatgga
    28081 aatggtataa attcctaatc gttttggcac taactcaatc ttatcttttt tgggaataat
    28141 gattgatgag cccaaggttc taaagacacg gttattaaga ctggaaattt ccgtcaattg
    28201 cagcgcttca atcgtcggat gcaagacagc accacctaaa gatttattgt aggctgtgct
    28261 ccctgtcggg gtcgatactg aaatcccatc acctcggaag ctttcaaatt tgacatggtt
    28321 aataataaca tctgctacca tcgttttttc aatacgctta accgtcgctt cattcaaagc
    28381 acgcgcttta accacacgac catcatctaa agtaatagca acttttaaaa taggataaga
    28441 gatttgttct cccttgtctt ttcttaaatt atcaatcaat ttatcaactt caaaatccct
    28501 ataatcggta taaaagccaa gatgaccggt gtggattcct acaaaacgta ccttatctaa
    28561 ttctttttca tacatgtgaa aggcagataa gagcatccca tctccgccaa tagaaatcac
    28621 aatatccgga tttttctttg aaagatagaa atcaggatca tctttaaata cggagaaaag
    28681 tttggaggcg acgcgtttac tttggtactt accatttgca ataatagcaa ctcgctttac
    28741 cttacctgta taattcatct gtgtcatcac tatttcctac accatcactt aaatttcgtg
    28801 tcacggggtc aaacaataat tgagcctccc gaatatcttc tcgaatctta cgcatttctt
    28861 cgtctaattc taaagcaatt ttagcagtca cttcaagacg ctttttaatt tcttctggga
    28921 agtctccacc atatttataa tttaaagaat gctcaatggt cgcccaaaag ttcattgcca
    28981 aagtacgaat ctgaatctcc gctaagacct ttttttgacc ttcaatagta tcaacagggt
    29041 actcaaccac cacatgataa gacctgtagc cactggactt catattgcga atataatctc
    29101 gttcataaac aattgtcatg tcttgtcgtt gacgcaatag agctaagact tcttccacat
    29161 catcaacaaa ctgaaccata atacgtagtc cagcaatatc ttgaatatct tgtgctatat
    29221 tttcttcaat aacaccacgt aaaatcattt tttctttaat actttcaatt gacttaacac
    29281 gtcccgtaac aaattcaatc ggagaataac gattttgctt acgatattgc ttgcgaatac
    29341 cccgtagttt tatttttaat tcaccaactg tttggatata aggatctaaa aattcttccc
    29401 aatctaaggt catctattgt ctcctttctt gtcaaagcca ggataataat ataaaattga
    29461 aataacaatt tattatagca caaattaaaa ggaatttccc atgactaacc tagaaatcga
    29521 atataaaact ctacttacaa aaaatgagta taaccgtctt ttatctcaaa tgaaacatgt
    29581 gacgcctgtt acacaaacta attactatat tgatacaaaa gcatttgatt taaaagctaa
    29641 caaaatgtca ttacgtattc gaacatttgt aaatagcgcc gaattaaccc taaaagtgcc
    29701 agaaaaagtg gggaatcgtg agtacaatgt ccctcttttt cttgaacaag caaaagatat
    29761 gattaaacat gggaatcttc ctgaatcaac tgctcttgat attattattt caaaaggtat
    29821 caaaccatct gcactggtaa cttttggcaa tttaactacc gttcgtcgcg agacagtgat
    29881 accgataggt aaattggcct tagactataa cctatatgcc aataccaaag actatgaatt
    29941 agaattagaa gtaagtgacg ctcttcaagg aaagattgat tttgattctt ttttatctga
    30001 acatcacatt actttcaaat atgctaaaag caaagtagct cggtgtatta ataccttaaa
    30061 aaagttcaac gataaataat ggctaaaagc gacagaaaaa ctgaaatttt tctgtttttc
    30121 tgttaaaata gggatgtcaa ttaaacactt ttgggtaatg tgattcatta ataaaacagt
    30181 ttacccaatt ttattgaagt taaggagcta aatctatcat gactgaacga tatgctgaca
    30241 agcaaatcaa actgttctca ctcacatcaa atcttccaat tgctgaaaaa attgctaaag
    30301 ctgctggaat ccctcttgga aaaatgtctt cacgtcaatt ttccgatgga gaaattatga
    30361 tcaatattga agaaacagtg cgtggagatg atatctatat tattcaatcc actagttttc
    30421 ctgtcaatga taatctttgg gaattactca tcatgattga tgcttgtaaa cgtgcaagtg
    30481 ctaataccgt caatattgta ttgccatact ttggttactc tcgtcaagac cgtgtcgcta
    30541 agccccgcga accgattact gctaaactag tagctaatat gctgactaaa gctggtattg
    30601 atcgtgtagt gacgcttgac ttacatgctg ttcaggtaca aggttttttt gatattccag
    30661 tggataacct ctttacagtc cctctttttg cagaacgtta cagtaaatta ggcttatcag
    30721 gttctgatgt tgttgtcgtt agccctaaaa attctggaat taaacgtgct agaagcttgg
    30781 ctgaatatct tgattccccg attgctatta tcgactatgc acaagatgat tctgaacgtg
    30841 aacaaggtta tattattggt gatgtttctg gtaaaaaagc cattttaatt gatgatattt
    30901 taaatactgg gaaaaccttt gcagaagctg ctaaaattct tgaacgctca ggcgctactg
    30961 atacttacgc ggtcgctagc catggcttat ttgctggtgg agctgctgat gtattagaaa
    31021 cagctccaat caaagaaatt atcgttacag attctgttaa aactaaaaat cgtgtgccag
    31081 aaaacgttac ttatcttagc gccagtgatt taatcgcaga agctattatt cgtatccatg
    31141 aaagaagacc cttaagtcca cttttttctt atcaacctaa aggcaaaaat aacgcatgac
    31201 ttactttgat aacgccgcca ccacaccact tagtcctaat gtgattaggg cgatgacagc
    31261 agctatgcaa gataactttg gtaacccctc tagtattcat ttctacgggc gccgagccaa
    31321 taaaatcctt cgtgaatgcc gacaagctat cgctagaaac ttgggggcaa gtgaacagca
    31381 aattatagta acgtccggtg gaactgaaag caataatatg gccataaaag gctatgcttt
    31441 agctcatcaa gcaaagggca aacatctcat cacaactact attgagcacc actctgttct
    31501 tcataccatg gcctatctcg aagagcgatt tggttttgag gtcacttatt tgccttgtca
    31561 aaatggacaa ataaatttat ctgacttaaa acaagccttg agagatgaca ccattttggt
    31621 aagcataatg tatgccaata acgaaacagg tgacctgtta cctattaagg acattgggaa
    31681 tctgctcaaa gaccatcaag ctgcttttca tgttgatgcc gttcaagctg ttggcaaact
    31741 caagattatt cctagcgaac ttgggattga ctttttatct gcttctgctc acaaatttca
    31801 tggtcctaaa ggatgcggct ttttatacag taacggacaa ccaatcgacc ctcttcttca
    31861 cgggggtgat caagaaggaa agcgacgcgc aagtactgaa aatatgttag gtattattgg
    31921 tatggcacaa gctttgactg atgctatgac ttgtcttgac caatcaactg atcacattat
    31981 cagtttacgt catcacttga ttagcctcct agaaggacta ccctactata ttaatcaagg
    32041 tactcattat cttcctcatg ttctcaatat tggattttta ggctaccaaa atactatttt
    32101 actaacacaa cttgacttag caggcattgc agtatcaact ggttctgctt gtactgctgg
    32161 agctgttaat ccaagtcatg ttttagctgc gtactatggg gatgactctt ctcgtttaaa
    32221 agaatcaatt cgtatcagtt tttcagatca aaatagcatt gaagatgtta atcaattagc
    32281 tcaaacatta aaaaatattt taggaggaac agatggcttt tgaaaaagaa attaagctta
    32341 aagactgtaa atacctttat acaatcagtc caaacattaa aaaatatacc ctaagagata
    32401 ctacatttag tcaaactaaa gtagggcact accaattaat acgtttactc gaaacaatac
    32461 ctaattcagg ggatggtttc cctttaaaaa tcactattaa taaagaactc acaggtttta
    32521 aattagctat ca
//
LOCUS       DAUCWU010000011        32266 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUCWU010000011 DAUCWU010000000
VERSION     DAUCWU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32266
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.011"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            230..2962
                     /locus_tag="VGY24_000514"
     CDS             230..2962
                     /locus_tag="VGY24_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ4283523.1"
                     /translation="MINKKCIISVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGTDVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVRVGDVLTISGEVEEFFGHGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGCVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      3127..3242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3240..3740
                     /locus_tag="VGY24_000515"
     CDS             3240..3740
                     /locus_tag="VGY24_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ4283524.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            3934..5892
                     /gene="ligA"
                     /locus_tag="VGY24_000516"
     CDS             3934..5892
                     /gene="ligA"
                     /locus_tag="VGY24_000516"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ4283525.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            5906..6928
                     /locus_tag="VGY24_000517"
     CDS             5906..6928
                     /locus_tag="VGY24_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ4283526.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIAKKFAHEVEDL
                     EQELEE"
     gene            7321..7518
                     /locus_tag="VGY24_000518"
     CDS             7321..7518
                     /locus_tag="VGY24_000518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ4283527.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            7553..8269
                     /gene="atpB"
                     /locus_tag="VGY24_000519"
     CDS             7553..8269
                     /gene="atpB"
                     /locus_tag="VGY24_000519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ4283528.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            8287..8781
                     /gene="atpF"
                     /locus_tag="VGY24_000520"
     CDS             8287..8781
                     /gene="atpF"
                     /locus_tag="VGY24_000520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ4283529.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            8781..9317
                     /locus_tag="VGY24_000521"
     CDS             8781..9317
                     /locus_tag="VGY24_000521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ4283530.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            9333..10841
                     /locus_tag="VGY24_000522"
     CDS             9333..10841
                     /locus_tag="VGY24_000522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ4283531.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            10857..11732
                     /locus_tag="VGY24_000523"
     CDS             10857..11732
                     /locus_tag="VGY24_000523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ4283532.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            11894..13300
                     /gene="atpD"
                     /locus_tag="VGY24_000524"
     CDS             11894..13300
                     /gene="atpD"
                     /locus_tag="VGY24_000524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ4283533.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            13313..13729
                     /locus_tag="VGY24_000525"
     CDS             13313..13729
                     /locus_tag="VGY24_000525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ4283534.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTIVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            13995..14252
                     /locus_tag="VGY24_000526"
     CDS             13995..14252
                     /locus_tag="VGY24_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ4283535.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            14317..15588
                     /gene="murA"
                     /locus_tag="VGY24_000527"
     CDS             14317..15588
                     /gene="murA"
                     /locus_tag="VGY24_000527"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ4283536.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLIEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            15592..15780
                     /locus_tag="VGY24_000528"
     CDS             15592..15780
                     /locus_tag="VGY24_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ4283537.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            15816..16355
                     /locus_tag="VGY24_000529"
     CDS             15816..16355
                     /locus_tag="VGY24_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEQ4283538.1"
                     /translation="MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFK
                     PAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKP
                     YSTGQNYYEGLVRRALDKGHSVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
                     NVQAGLTVDYRTGQIAINL"
     gene            16638..17681
                     /gene="pheS"
                     /locus_tag="VGY24_000530"
     CDS             16638..17681
                     /gene="pheS"
                     /locus_tag="VGY24_000530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ4283539.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQTKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVENDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            17891..20296
                     /locus_tag="VGY24_000531"
     CDS             17891..20296
                     /locus_tag="VGY24_000531"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ4283540.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPAEGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPEKNLQESDKATSEAIEVAIASDN
                     VLTYSSRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRELTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYSYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESVGVK
                     RLTKIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            20406..20783
                     /locus_tag="VGY24_000532"
     CDS             20406..20783
                     /locus_tag="VGY24_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ4283541.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            20776..21162
                     /locus_tag="VGY24_000533"
     CDS             20776..21162
                     /locus_tag="VGY24_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ4283542.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            21235..22311
                     /locus_tag="VGY24_000534"
     CDS             21235..22311
                     /locus_tag="VGY24_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283543.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITANKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            22321..22989
                     /locus_tag="VGY24_000535"
     CDS             22321..22989
                     /locus_tag="VGY24_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283544.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTYLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGIAGLKHQLPQELSGGERQRAAIARALYHDPA
                     LVLADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLEYCDKVYRMQDGEL
                     RQER"
     gene            complement(23093..24010)
                     /locus_tag="VGY24_000536"
     CDS             complement(23093..24010)
                     /locus_tag="VGY24_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ4283545.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFNIPYSKL"
     gene            24161..27376
                     /gene="rexB"
                     /locus_tag="VGY24_000537"
     CDS             24161..27376
                     /gene="rexB"
                     /locus_tag="VGY24_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ4283546.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLEKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     QIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDHERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVSTVINETFDDLSPYLKELINFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIQVIDQE
                     KNFQLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGTYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGYFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMGQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            27337..31005
                     /gene="addA"
                     /locus_tag="VGY24_000538"
     CDS             27337..31005
                     /gene="addA"
                     /locus_tag="VGY24_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ4283547.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERYSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFNEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTDLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQE
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADMFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKATYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLSLSKPINQQTL
                     LDALRGIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            31145..31957
                     /locus_tag="VGY24_000539"
     CDS             31145..31957
                     /locus_tag="VGY24_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4283548.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            32098..>32266
                     /locus_tag="VGY24_000540"
     CDS             32098..>32266
                     /locus_tag="VGY24_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ4283549.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRK"
BASE COUNT        10150 a         5616 c         6714 g         9786 t
ORIGIN      
        1 ataaagctgt cttgttcgct atcaggggtg cttgtgatgt tatgtcagat caaacaagtc
       61 tttaattggg tgacttaaca gattaaaaga aaagatctaa tcgtgttact tgatagtata
      121 attaatgcaa acagaaagtg tatcccaatt catagtctta ggtggaggtt aatattcaaa
      181 aataaaatct tgttgttgtc ttaaactaat cacattaaaa ggagatatca tgattaacaa
      241 gaaatgtata atatctgttt cattgttgac actagctatt acgcttacta gtgttgaaga
      301 agttacttca cgccaaaatt tgacttatgc caatgaaatc gtaacacaaa ggccaaagag
      361 agaatctgtt attagtgata aatcgaattt tcccgtcata tcaccttacc tagcaagtgt
      421 ggattttggt gagagaaaaa catctttgcc aacacctgat aaaggagtaa aagtaactac
      481 tgaacagtct attgctcaag taagaaaggg gcctgaagaa agaacctata ctgttactgg
      541 caagattacg agtgtgatca atggctgggg aggctatggc ttttatattc aagatagtga
      601 aggtattgga ctttatgttt atcctcaaaa agatttagga tacagtaagg gagatattgt
      661 tcaattaaca ggcacactta ctcgctttaa aggtgattta caactccaac aggtgactgc
      721 acacaaaaag ttagagttat cttttccgac ttctgttaaa gaagcagtaa tatcagaatt
      781 agaaacaaca acaccctcaa cattagttaa gttatctcac gtgacagttg gagaattatc
      841 aactgatcaa tataacaaca catctttcct tgtaagggat gatagtggta aaagtatagt
      901 tgttcatata gatcatcgta caggggttaa agggactgat gttgttacta aaataagtca
      961 gggtgatttg attaacctca cagccatatt gtctattgtt gatggtcagt tacaattaag
     1021 accgttttct cttgaacaat tggaagtggt taaaaaggtc acaagctcaa atagtgatgc
     1081 ttcatctcgt aatattgtga aaataggcga gattcaagga gctagtcata cgtcgccact
     1141 tctcaaaaaa gcggtcaccg tagaacaggt tgttgtcact tatttagacg attccactca
     1201 tttttatgtt caagatctta atggtgatgg tgatttagcg acttcagatg gtattcgtgt
     1261 ttttgctaaa aacgctaagg ttcgagtcgg cgatgttttg accatttcag gtgaagtgga
     1321 agaattcttt ggtcatggtt atgaggaacg taagcagact gaccttacca tcacccaaat
     1381 tgtggctaaa gcagtgacca aaacagggac agctcaagtt ccatcaccgc ttgttttagg
     1441 gaaagatcgt atcgcgccag ccaatattat tgataatgat ggcttgcgtg tgtttgatcc
     1501 agaagaagac gctattgatt attgggaatc aatggaaggc atgttagtgg cggttgatga
     1561 tgctaaaatc cttggtccaa tgaaaaataa agaaatttat gtcttacctg gctctagtac
     1621 aagaccgtta aataattcag gtggagtatt acttccagct aattcttata acacagatgt
     1681 gattcctgtt cttttcaaaa aaggcaaaca aattattaaa gcaggagact cttacaaagg
     1741 aagattagct gggccagtat cttatagcta tggtaattac aaggtctttg ttgatgacag
     1801 caaaaacatg ccaagtttaa tggatggtca tctaaaacct gaaaaaacaa acttgcaaaa
     1861 agaccttagc aagttaagca ttgcttctta caatattgaa aacttctcag ccaatccttc
     1921 ttcaactaaa gatgagaagg tcaaacggat tgccgaatcc tttattcatg atctgaatgc
     1981 tccagacatt attggattaa ttgaagtcca agataataat gggccgactg atgatgggac
     2041 aacggatgcg acacaaagcg cacaacgcct cattgatgct attaaaaaac taggtggccc
     2101 aacttatcgt tatgttgata ttgctccaga aaataatgtt gacggaggtc aaccaggtgg
     2161 taatattcga acaggattcc tttatcaacc agagcgcgtc agcctttctg ataagccaaa
     2221 aggcggtgct cgtgatgctc taacttgggt taatggagaa ttaaacctta gtgttggtcg
     2281 aattgatcca actaacgccg cttggaaaga tgttcgtaaa tcactagcag cagaatttat
     2341 cttccaaggt cgtaaagtcg ttgttgttgc aaatcatttg aactctaagc gtggggataa
     2401 tgctctttat ggttgtgtgc aaccagtcac ttttaaatct gagcaaagac gtcacgtctt
     2461 ggctaatatg ctagcacaat ttgcgaaaga aggggcaaaa caccaagcta atattgtgat
     2521 gctaggtgac tttaatgatt ttgaattcac aaagacgatt caattaatcg aagaaggtga
     2581 catggttaac ttggtgagcc gacatgatat ttcagatcgg tattcttatt ttcaccaagg
     2641 caataatcag acccttgata atatattagt ttcacgccat ttacttgatc actacgaatt
     2701 tgacatggtt catgtgaatt ccccatttat ggaagctcac ggacgcgcat cagatcatga
     2761 tccattgtta cttcaattat cattttctaa agaaaatgat aaggcagagt cttctaaaca
     2821 aagtgtaaaa gctaaaaaaa cttcaaaagg aaaactgttg ccaaaaacag gagatagtct
     2881 tgtttatgtg ataacgctac taggaacggc tagtttatta gtgcctattt tattattgac
     2941 taaaggcaaa aaggaatcat agcgatttga agaattggag ttcataaaga gtcgttaaag
     3001 gcttgatata cttacgttag accttaagct aaaatggatg actatataaa aactaatatt
     3061 gataacaaaa gaagataaca ataagacagc tttacttgct aaagactttt gtcaagctaa
     3121 ttgttttatg gtacaatgta accgttgctt tgcaaccctt ggtgcttagt tccctttcac
     3181 caagcatatt agaaacgtag atagcttttg agagttatct agacgttaaa ggagaaaaaa
     3241 tgacaaaatt gaccgtacac gactatgttc acattgggct agtagctgcc ttgtatgttg
     3301 tcttaactat taccccgcct ttaaatgcga tttcttatgg gatgtatcaa tttagaatat
     3361 ctgaaatgat gaatttctta gctttttatc atcgcaagta tattattgcg gtgactcttg
     3421 gatgtatgat tgctaacttt tatagtttcg gactgattga tgtctttgta ggcggcggtt
     3481 caactcttat ttttgtgacc ttaggtgtta ttttgtttag taagtatcaa aaagattatc
     3541 tttttaatgg catttttaat aaagcttttg tttacttttc tttctttttt gcaacttcca
     3601 tgtttactgt agcaatcgaa ctttacttct ttggagctcc atttttatta acatggttta
     3661 caactgcgtt gggagaatta gtatctctgc tcataggatc acttattatt gataagttgt
     3721 ctcaaagaat ttccttttag taatgcatca tactcaaaag agttgggtca atacccttct
     3781 cttttttatg ttttttgtaa tgttaaaatc gttctagata gaaatattta acactttagc
     3841 taatagtacc atatggagtg gccaacgatt acttttgatg ttgttatgat atagaaatag
     3901 ctcatttgtt atcttttatg ataaaataaa agtatgaaaa aacgcataaa agaattaaca
     3961 gatcttctta accgttaccg ttatgactat tatactaaag atgccccatc agtttctgac
     4021 agtgactatg ataagcttta tcgtgaactg gtgactttag agcagtcata tcccgagtat
     4081 gtcttgcaag atagcccaac gcagcaggtt ggagggacta ttttaaaggg gtttgaaaaa
     4141 tatcgacatc agtatccgct ttttagtttg caggatgctt tttcacgtga ggagctggat
     4201 gcttttgata agcgcgtcaa ggccgaattt ccgaatgcca cctatctagc agagttaaaa
     4261 attgatggtt tatctatttc cttgagttat gagaatggct tcttacaggt tggagcaaca
     4321 cggggtgatg gcaatatcgg tgaaaatatc acagaaaata ttaaaaaaat aaaggatatt
     4381 cctcaccaat tgagtgagcc tttaaccatc accgttcgag gagaggctta catgtctcgt
     4441 cagtccttta aagcgatcaa tgaagcacgc caagaaaatg gggagactga atttgctaat
     4501 cctagaaatg ctgccgctgg gacactaagg cagctagata cggcagtggt tgctaagcgt
     4561 caactagcca cttttttgta tcaagaagct agtccgacag ctagaaatca acaaaatgag
     4621 gtcttagcag aattagcaga tctaggtttt tcagtaaatc cgtattatca actcacatcg
     4681 tctatggatg agatttggaa ttttatcaaa actattgagg caaaaagaga tcaattagct
     4741 tatgacatag atggcgttgt tattaaagtt aatagcctag ccatgcaaga agagctaggg
     4801 tttacggtta aagctccacg ctgggctatt gcttacaaat ttccagcaga agaaaaagag
     4861 gcagaaatcc tttcggttga ctggacagtg ggacgaacag gcgtggtcac acctacagcg
     4921 aatctaactc ctgtgcaact agcggggaca accgtgagtc gtgcaaccct acataatgtg
     4981 gattatattg ccgaaaaaga tatccgtatt ggcgatacag tgattgtata taaggcaggg
     5041 gacattattc ctgctgtctt aaatgtcgtt atgagtaagc ggaaccagca agaagtcatg
     5101 cttattccta aactatgccc atcttgtggt agtgaattag ttcattttga agatgaggtt
     5161 gctttgcgtt gtattaatcc gttgtgtcct agtttgattc agcgaagttt ggagcatttt
     5221 gcaagtcgtg atgccatgaa cattactggg ctgggaccag ctattgtgga aaaactattt
     5281 ttagcggggt ttgttcatga tgttgctgat atttaccagt tgaccaaaga agatttcatg
     5341 caattagatg gcatcaaaga aaagtcagct gataaattgc tcgctgcgat tgaggcttct
     5401 aaatcaaatt cagctgaaaa actgttattt ggtctaggaa ttcgtcatat tggctctaag
     5461 gttagtcgct taattttaga ggtatacgga gatatttctg ctcttttaac cgctaaagaa
     5521 gaggaaatcg caaggataga tggtcttggc tcaacgattg ctcaatcact tactcaatac
     5581 tttgaacaaa aaacggctgc gattttagta gacgagttaa agacagcagg tgtcaacatg
     5641 cattactcag gtcaaaaagt taatagtgac gcagcgcttt ttggtctaac tgttgtttta
     5701 actggcaaat taaatcagct gaaccgaaat gaggccaaag ataaattaga ggcattaggt
     5761 gccaaagtta ctggaagtgt atctaagaaa acagatttag ttattgcagg tagtgacgct
     5821 ggttctaagc tagaaaaagc aaagagctta ggaattcgga ttgaagatga agattggcta
     5881 cgccagcttt aagtgaggta atgttatgaa aaaacaattg agagcacgtc tcatttataa
     5941 tccaacgtca ggacaagaac ttatgagaaa aagtgttcct gaagttttag acatattaga
     6001 agggttcggg tatgaaacat ctgcctttca aacaacagcc caaaagaact ctgctctaaa
     6061 tgaagcaaga agagcggcaa aggcaggttt tgacttattg attgctgctg gaggtgatgg
     6121 cactattaac gaagtggtca atggaattgc tcccttgaaa aagcgcccta aaatggccat
     6181 tattccaaca ggtaccacta atgattttgc tagagcgtta aaagtaccaa gaggaaatcc
     6241 gagccaagca gcaaaactta tcggaaaaaa tcagactatc cagatggata ttggtagagc
     6301 taagaaagat acttatttca tcaatattgc tgcagcagga agtttgacgg aattaactta
     6361 cagtgtacca agtcagttaa aaacgatgtt tggatacttg gcttatcttg ctaaaggagt
     6421 agaactgctc ccaagagtta gcaatgtgcc tgttaaaatt acgcatgaca aaggcgtctt
     6481 tgaaggtcaa gtttctatga tttttgcagc tattaccaat tctgttgggg gatttgaaat
     6541 gattgctcct gatgcaaaac tagatgatgg tatgtttacg ctcattttaa taaagacagc
     6601 aaatcttttt gaaattgtac atcttttgcg tttgattttg gatggtggta agcatattac
     6661 agatcgccgt gtcgaataca ttaagaccag taaaatagtc attgaacccc aatgtggtaa
     6721 gcgaatgatg attaatttag atggcgagta cggaggagat gctccaatta cattagagaa
     6781 cctaaaaaat catatcactt tttttgcaga tactgatttg atttctgatg acgctttagt
     6841 cttagatcag gacgaattgg aaattgagga aatcgcaaag aaatttgctc atgaagtaga
     6901 agacttagaa caagaattag aagaataata caatagttat tagctactga cagtttagtc
     6961 aaatgcacta ctattagaat ctaagatttt taaatcaatt gaggtagtgt tttttgatgc
     7021 gctcaaagag tcaatttatt catactgagg ggcccatttg aggtctctct tttttgatca
     7081 tatgcaagaa aatgccatgt tttaggggtg ttattatacc ctttttggaa tcttgttgcc
     7141 tttttgttat aataagagtg taattctatt tttaaaaaga tcttaacaaa gacagactcg
     7201 tttatcaaaa aggttagtat tagtctaaga aagcgtactc agtctaaaga aatctgttga
     7261 cattcttaat tttaaggaat agaatagcaa agtaaaaaaa atttaaagga gtattctact
     7321 atgaatccta ttttcgcatt ggctttggcc tgttttggtg tttctcttgc cgaaggtttc
     7381 ttgatggcaa atctttttaa agcagcatct cgccaaccag aaatcattgg tcaattacgt
     7441 tcattaatga tcttgggtgt tgcctttatc gaaggtactt tctttgttac ccttgttatg
     7501 gccttcattc ttaaataaca gcctatttga taatagaaaa ggagggtgtt agatggaaga
     7561 agctaaaata cccatgctaa agcttggtcc aataaccttt aatttgaccc tacttgctgt
     7621 ttgtattgtc acaattgctg ttatctttgc ctttgttttt tgggcaagtc gccaaatgaa
     7681 attgaaacca gaagggaagc aaactgcttt agaatattta atcagttttg tggatggtat
     7741 tggagaagag cacttagatc ataatctaca aaaatcttat tcgctgttac tctttaccat
     7801 ttttctcttt gtggctgtcg ctaataattt agggttattt actaagttag agacagttaa
     7861 tggctataac ctatggacct cgccaacagc caatctggct tttgaccttg ctctatctct
     7921 ttttattacc ttaatggtac acattgaagg ggttagacgt cgtggcttgg ttgcccattt
     7981 gaaacgtttg gctacaccgt ggccaatgac tccgatgaat ttattagaag agttcacaaa
     8041 tttcttatca cttgccatta ggttattcgg taatatcttt gccggggaag ttgttacagg
     8101 tttgattgtt caactggcca attatcgagt ttattggtgg ccgatcgctt tcctagtcaa
     8161 tatggcgtgg acagcctttt cagtctttat ttcctgcata caggcttttg ttttcacaaa
     8221 actaacagcg acctatctag gaaagaaagt taatgaatca gaagaataag gaaggagaac
     8281 aaacgaatgt caatcacatt tggtgaactc gttgggaatt ttattctagt aacaggttct
     8341 gtaatcgtct tgttactctt gatcaaaaaa ttcgcttggg gtgcaattga atcgatttta
     8401 cagacacgct cacaacaaat ctctcgagat attgatcagg ctgagcaatc acgtctaagt
     8461 gctcaacagt tagaggcaaa aagtcaagct aacctagatg ctagtcgttc acaagcaagt
     8521 aaaatcatta gtgatgccaa agaaattggt caattacaag gtgataaatt ggtggcagaa
     8581 gctactgatg aagcaaaacg cttgaaagaa aaagcgttga cagatattga acaaagcaaa
     8641 tcagacgcta tttcagcagt caaaacagaa atgtctgatt taacggttct tttagcggaa
     8701 aaaattatgg gagccaatct tgataagacg gcgcaaagcc agcttattga cagttatctt
     8761 gatgacttag gagaagctta atgaccaaaa aagaacaggc tcttatcgag caatatgcaa
     8821 aaagccttgt tgaggtagca agtgaacatc attctcttga tgccttacaa gctgatgtgc
     8881 ttgcgatttt agagacgttt gtaacaacta atcttgatca aagtctatca agtctagctg
     8941 ttccgcatgc tgaaaaaata aaattgctaa ccttattaaa gggaaataat tctgtttata
     9001 tgaataattt cttgaatctc attttacaaa acgaacgtga agcttatttg tatcaaatgc
     9061 ttcaggctgt tttaaatgaa attgcaatag tttcaaatca gtatgatgtg actgttacct
     9121 catcactacc tttgactgag gagcaaaaaa gccgtgttcg agcagttgtt gcaaagaaat
     9181 ttgctgtaac agcgggtcgt ttaattgaaa aagtagatcc ttcgcttatt ggaggattta
     9241 ttatcagcgt caataataaa gtaattgata ccagtattcg tcgtcaattg caagcattta
     9301 aaatgaattt aaaatagaaa gtggtgtgac ttttggcaat taatgcacaa gaaattagcg
     9361 ctttaattaa aaagcaaatt gagaacttcc agccaaattt cgatgtcaca gaaactggga
     9421 tcgttaccta tattggtgac ggtattgcac gtgcccgtgg attagataat gccatgagtg
     9481 gtgaacttct tgaatttgaa aatggtgcct acgggatggc tcaaaacctt gagtcaaatg
     9541 atgtgggtat tattattctt ggtgattttt cagcaatccg tgaaggagat gtggttaagc
     9601 gcaccggtaa aatcatggaa gtaccagttg gtgaagctct cattggacgt gtggtaaacc
     9661 cacttggaca accagttgat ggcttgggag atattgaaac aactggcttt agaccagtag
     9721 aaacgccagc tcctggtgtt atgcagcgta aatccgtttc tgaaccctta caaactggat
     9781 taaaagcaat tgatgctttg gttccaattg gtcgtgggca acgtgagttg attatcggag
     9841 accgtcaaac aggaaaaaca tccgtagcta ttgatgcgat tttgaaccaa aaaggtcaag
     9901 acatgatttg tatctatgtg gctattggtc aaaaagaatc aaccgttcgt acacaagttg
     9961 aaacccttcg ccgttacggt gccttggatt atactattgt tgtgacagcg tcagcctcac
    10021 aaccatcacc attgctcttc atcgctcctt atgcgggtgt tgccatggct gaagaattta
    10081 tgtaccaagg caagcatgtc ttgattgtct atgatgattt atcaaaacaa gcggtggctt
    10141 atcgtgaatt gtctctcttg cttcgtcgtc caccaggtcg tgaagcttac ccaggggatg
    10201 ttttctactt gcatagccgt cttttggaac gctctgcaaa agtttctgat gatttaggtg
    10261 gaggttctat cacagctcta ccatttatcg aaacacaggc cggagatatc tctgcatata
    10321 ttgcgactaa cgttatttcg attacagacg gacaaatctt cttgcaagag aacctcttta
    10381 attcaggaat tcgtccagcg attgatgcag gatcatctgt ctcacgtgta ggtggttcag
    10441 ctcaaattaa agcgatgaaa aaggttgctg gtactcttcg tttggatttg gcttcctatc
    10501 gtgaattaga agcctttacc caatttggtt cggaccttga tgctgcaaca caagcaaaac
    10561 ttaatcgtgg tcgccgtacg gttgaaatcc tgaagcaacc attgcataaa ccgttacctg
    10621 ttgaaaagca agtcgttatt ctttatgccc ttactcatgg tttcttagat gatgttcctg
    10681 tagatgatat tttggctttt gaagaagctc tttacgacta ctttgacgtt cattacaatg
    10741 atttatttga aactatccgt accacaaaag accttcctga agaagctgcc cttgatgcgg
    10801 ctattaaagc gtttaaagaa cactcaaact ttaagtcata gagggaggat actagtatgg
    10861 caggctctct aagtgaaatt aaagctaaaa ttatttcaac tgagaaaaca agtaaaatca
    10921 ctagtgccat gcgtatggtt tcttctgcaa aacttgttaa atctgagcaa gcagctcgtg
    10981 attttcaaat ttacgcctca aaaattcgcc agattaccac agatttattg aaatcagaat
    11041 taaccatagg atctgataat ccgatgttgg tgtctcgtcc tgtcaaaaaa acaggctata
    11101 ttgtcatcac gtctgataaa ggacttgttg gaggttacaa ttcaaaaatc ttgaaatctg
    11161 ttatggatat gattacagag taccatgctg atggtgacta tgaaatcatt tctattggta
    11221 gtgttggttc agacttcttc aaagcaagag ggatgaatgt ggcttttgaa cttcgtggac
    11281 ttgcggatca accaagtttt gaacaagtga gacaaatcat ttcacaatcc gttgacatgt
    11341 ttgtcaatga gatttttgat gagttatatg tctgttacaa tcaccatgtg aatagcttaa
    11401 ccagccaagt ccgtgttcaa cagatgttgc caatttctga cttggttgcc gatgaagcag
    11461 cagaagaagg tgttactggg tttgaattgg aacctaatcg tcatgatatt ttagatcaat
    11521 tgttacctca attcacagaa agtttaattt atggggctat cattgatgct aaaacggctg
    11581 aacatgcagc aggtatgact gccatgcaga cagcaactga taacgctaag aatgtcatta
    11641 acgatttgac catccaatat aaccgagctc gtcaagcagc aattacacaa gaaatcactg
    11701 aaattgttgc aggagcaaat gcactagaat aaaaaatgac gctgactagc gattaggtta
    11761 ttgtcagcat tcaattttat caatcgagat aaaatgactt atgggacttt tctattgaaa
    11821 gactgctctt ttcagtataa aagaagttag caagactaac taagccctga ctatcgaata
    11881 taaggagaaa tgaatgagct caggcaaaat tgctcaggtt gttggtccag tagtagacgt
    11941 tatgtttgct agcggggaca aacttccaga gattaataat gcattgattg tttataaaga
    12001 tagtgataaa aagcaaaaaa tcgtccttga agttgctctg gaacttggtg acggtatggt
    12061 gcgaacaatc gctatggaat caactgatgg gcttacacgt gggttagaag ttcttgacac
    12121 tggtcgtgcg attagcgtac cagtaggtaa agaaactttg ggacgcgtct ttaatgtact
    12181 tggagaaacc attgacttgg aagaaccatt tgcagaagat gttgaccgtc agccgatcca
    12241 taaaaaagca ccatcgtttg atgaattatc aacatcatca gaaattcttg aaactggtat
    12301 caaggtaatt gaccttcttg ccccgtacct taaaggtggt aaagttggac tttttggtgg
    12361 tgccggagtt ggtaagactg tccttatcca agaattaatc cacaatatcg cccaagaaca
    12421 cggaggtatt tcagtattta ccggtgttgg tgagcgaaca cgtgaaggaa atgaccttta
    12481 ctgggaaatg aaagaatcag gcgttattga aaaaaccgcc atggtttttg gtcagatgaa
    12541 tgagccgcct ggggcacgta tgcgtgtagc ccttactggt ttaaccattg ctgagtattt
    12601 ccgtgatgta gaaggccaag atgttttgct ctttattgat aatatcttcc gtttcactca
    12661 ggcaggttca gaagtatcag ccctcttagg ccgtatgcct tctgctgttg gttaccaacc
    12721 aacccttgct actgaaatgg gacaattgca agaacggatt acatcaactc aaaaaggttc
    12781 tgttacatct atccaagcga tttatgtacc agcagatgat tatactgacc ctgcgccagc
    12841 aacagcattt gctcacctgg attcaacaac taaccttgaa cgtaaattga cacaaatggg
    12901 gatttatcca gccgttgatc cattagcttc aagttcacgt gctttatcac ctgagattgt
    12961 tggtgaagag cactatgctg tggcaacaga agttcaacgt gtgcttcaac gctaccgtga
    13021 attacaagac atcatcgcta ttttggggat ggatgaatta tcagatgaag aaaaaacatt
    13081 ggttggacgt gcgcgtcgta tccagttctt cctctcacaa aattttaacg ttgctgaaca
    13141 atttaccggt cttcctggtt cttacgtgcc agttgctgag acagttcgcg gctttaaaga
    13201 aattcttgaa ggaaaatacg atcaccttcc agaagacgcc tttcgttcag ttggtccaat
    13261 tgaggatgtg attaaaaaag ccgaaaaaat gggcttttag tgaggtgatg ccatgacaca
    13321 aatgactgtt caagtggtaa cgccagatgg aatcaaatat gatcaccatg ctaaatgtat
    13381 ttctgtaaca acaccagatg gggaaatggg gattttacct aatcacatca atttaattgc
    13441 acctttgcaa gtccatgaaa tgaaaattcg ccgaggtggt gaagatgaaa aagtggactg
    13501 gattgcaatc aatggtggta ttattgaaat caaggataat gtggttacca ttgtcgctga
    13561 ttctgcagaa cgtgaccgtg atatcgatgt cagccgagca gagcgagcaa aactaagagc
    13621 agaacgtgaa attgcacagg ctgaaacgac ccacaacatt gatgaagttc gtcgtgccaa
    13681 ggttgccttg cgccgcgctt tgaatcgtat caacgttagc aaaaaataag tggtgttgat
    13741 aaaagacaag gataagtaag aaaggttttt aacctattat tttgtttgct gagtcttttt
    13801 aacatcagtt tctgagagtt tgaccatgct accttctcta aacacgttat tattgttgag
    13861 aggagttata tgggggaact cttttttgta tccacgttgt tagtagaaac tcagtaatga
    13921 cgcttaatgt atttttaggc tatcaactta gtcagagagg atgtcaattt aattgataag
    13981 ttggacggat caagatggta tgttataata atcctatgga attattaatg aagatcattg
    14041 ccttttcgtg tcatttattt tttattggcc ttagttacca attattgatt agtgtgattg
    14101 actggacaaa atttagtcat tatcatccag aaaatcttgg ccggctacga ctttttgtct
    14161 ttttattagc tactgcccta gggtatttgg tgagccattg tatgctggaa ttgcttcaaa
    14221 ttagccagac gctctttttt gagttccgct aatactggaa taaaagaagt atggtataat
    14281 tttactacga ctaagagata gataagggag aaaattgtgg ataaaataat cattgaaggt
    14341 ggtcaaacac gcttagaagg agaagttgtt attgagggtg ctaaaaacgc agttcttcct
    14401 ttactggcag catctatttt accttcaaaa ggaaaaacaa ttcttagaaa cgttcctatt
    14461 ctatctgatg tttttaccat gaataacgtg gtacgtggtt tagatattcg tgttgatttt
    14521 aatgaagcag ctaacgaaat cacagttgat gcctcaggac acattttaga tgaagcccct
    14581 tacgagtacg tgagccaaat gcgtgcctct attgttgttt taggaccaat tttggcacgt
    14641 aatggccacg caaaagtatc aatgcctgga ggttgtacga ttggcagtag gcctattaat
    14701 ttgcacttaa aaggattaga ggctatgggg gcaacgatta ctcaaaaagg tggagatatt
    14761 accgctcaag cagatcgact gcaaggtgcc atgatttata tggacttccc atctgtagga
    14821 gctacccaaa acttgatgat ggctgccact ttagctgatg gtgtgaccac tattgaaaat
    14881 gctgctcgtg agcctgaaat tgttgattta gctcaatttt tgaataaaat gggagctaga
    14941 attcgtggtg ctggaacaga gacgttaacg ataacaggcg tcacgcattt acgaggcgta
    15001 gaacatgatg ttgttcaaga tcgtatagaa gccggtacat ttatggttgc agcggcgatg
    15061 acttcaggca atgttttgat tagagatgct gtttgggaac ataatcgtcc attgatttca
    15121 aaattaatag aaatgggtgt ttctgtaaca gaagaagaat acggtattcg cgttcaagca
    15181 aacacgccaa agctcaaacc tgtaacggtt aaaaccttgc ctcatccagg ttttccaaca
    15241 gacatgcaag ctcaatttac agctttaatg gcagtggtca atggagagtc tactatggtt
    15301 gaaactgtct ttgaaaatcg atttcaacat ctggaagaaa tgcgacgcat gggcttacaa
    15361 tctgaaatcc ttagggaaac tgccatgatt catggtggaa gacaactgca aggtgctcct
    15421 gtgatgtcaa cagatttacg tgccagtgct gcccttatct tgacagggat tgttgctcaa
    15481 ggagttacaa tagttaataa tttggttcat ttagatcgtg gctactatca atttcatgaa
    15541 aaattagcaa agcttggagc tactattagt cgtagcagtg aggtctagct tatgacaaat
    15601 ggtttaaaat atgtcttaga acaaatgtta ttacttttca taattgctgc cttggcgtgt
    15661 ctttttttag ccattggact tatgattggt tatagtttta tgggagatgg ccatagtcca
    15721 tggcatattt tatctatgga taagtgggca gagttagtga ataaatttac tggaaagtag
    15781 gccttgccta ctttttatag catctgagag gaagtatgtt aacaaggaaa aaacgaaaaa
    15841 gggtgcaaga gcaaaaggtt cctaaaagag ctaatgcctt acttactaag tcgacacgac
    15901 agtatcgaaa tcgctatcaa acggacaatg cttatcgtaa ctttaagcca gcaggttggc
    15961 accgattgca ccacttaaaa ggaagttatg atcacgcggt tgatagaggg catttgctag
    16021 gttatgcttt agttggtgga ctgaaaggtt ttgatgcttc tactggtaat cctgataata
    16081 ttgccactca actaagttgg gctaatcaag caaataaacc ctactcgaca ggtcagaatt
    16141 actatgaagg tttagtacgt cgtgctttgg ataaggggca tagcgtccgc taccgtgtca
    16201 ccctacttta tgatggtgat aatctactag ccagcggtag ccatttggag gctaaatcct
    16261 ctgatgacag cttgactttt aacgtgtttg ttccaaatgt ccaagcagga ttgaccgttg
    16321 actatcggac gggacaaata gctatcaatc tctaagcgaa aagtcactga tttaagattg
    16381 tcactaagca ctttttttga tagaatagaa ctaattgaat aagtgtctat gagactagta
    16441 ctaatagaga aattaacagg gagtgagggc cgtgactgga agccttctta agggaactat
    16501 taggaattca ctcagctaaa gacaattaat taaggtctga taacatcaga taaaaacgga
    16561 tggtaccgcg tgtcaacgct ccgcttatca agtggaggag tggcatgctt ttttcatcgc
    16621 caaaagaagg gaaaactatg gatttacaag cacaattaga ggaattaaag acaaaaacat
    16681 tggagacctt acagtctttg acgggtaatc ataccaaaga actgcaagat ttgcgcgtgg
    16741 ctgttcttgg gaaaaaggga tctttgacag aattactaaa ggggctaaaa gacttgtcta
    16801 atgacttgcg tcctgtggtt ggtaaacagg tgaacgaagt tcgtgactta ttgacaaaag
    16861 cctttgaaga acagaccaaa attgtagaag ctgctaagat tcaagcccaa cttgatgctg
    16921 aaagcattga tgtgacttta ccgggtcgtc aaatgactct gggccaccgt catgtgttaa
    16981 cacaaaccag tgaagaaatc gaagatattt tcttaggaat gggttttcag attgttgatg
    17041 gctttgaggt agaaaacgat tattacaact ttgagcgcat gaatttgcca aaggatcacc
    17101 ctgcccgtga catgcaagat acgttttata tcacagaaga aatcttgtta cggactcata
    17161 ccagtcctgt gcaggcccgt actcttgatc aacatgattt ttcaaaaggc cctttaaaaa
    17221 tggtttctcc aggtcgtgtt ttccgtcgag acaccgatga tgctacccac tcacatcaat
    17281 tccatcaaat tgaaggtttg gttgtgggca aaaatatttc catgggagac ctcaaaggga
    17341 ctcttgaaat gattatcaag aaaatgttcg gtgacgaacg tagcattcgt ttgcgcccgt
    17401 catatttccc gttcacagaa ccatctgttg aagtggatgt ttcttgcttt aaatgtggtg
    17461 ggaaaggctg taatgtctgc aagaaaacag gatggattga aattcttggt gctggtatgg
    17521 ttcacccaag tgtcttggaa atgtcaggtg ttgatgcaaa agagtattca ggctttgcct
    17581 ttggtttagg tcaagagcga attgccatgt tgcgttatgg cattaatgac attcgtggtt
    17641 tctatcaagg cgaccaacgt ttctctgaac aatttaacta aaacgagtcg tttgaagacc
    17701 tctgtcagct attttatcta agattgagtg aaagttggta ggtaatgata ataggttaat
    17761 tgtctaaggg gaaagtccct ctcgcttcaa cgtcataagg gttttgactt ggaacttaaa
    17821 atacgaaagg aaatgactca aatcacggct tgagagcttt ctctagccga gtgatttggg
    17881 cactaaaatt atgttagtat cttataaatg gttaaaagaa ttagtagata tcgatgtgac
    17941 accagcagca cttgctgaga aaatgtcgac aacgggtatc gaggtagaag gaattgaagt
    18001 ccctgcagaa ggtttatcaa aactggttgt ggggcatgtg ctttcttgtg aagatgtgcc
    18061 agagacacat ttgcatttgt gtcaggtaga tacaggtgat gaaacgccac gtcaaattgt
    18121 gtgtggcgca ccaaatgtga aagcagggat taaggttatc gtggctgtgc caggtgctcg
    18181 tattgcggat aactataaaa tcaaaaaagg caaaatccgc ggcatggaat ctcttggtat
    18241 gatttgttcc ttgcaagaac ttggtttgtc agatagtatc attccaaaag aattttcaga
    18301 cggtattcaa atcttgccag aggaggctgt gccaggtgat gctattttta aatacctgga
    18361 cttggacgat catatcattg aattgtccat tacacctaac cgtgctgatg cgctttctat
    18421 gcgaggtgtg gcacatgaag tcgcagctat ttatggcaag tcggtgtcat tcccagaaaa
    18481 aaatctacaa gaatctgata aggcaacttc ggaagccatt gaagtggcca ttgcctctga
    18541 taacgttcta acttactcta gccgtgtcgt tgaaaatgtc aaagttaagc caagtccaca
    18601 atggttgcaa aaccttttaa tgaatgctgg tattcgtcct attaataatg tggttgatgt
    18661 gaccaactat gtgctcttgt attttggtca acctatgcat gcttttgatt atgataaatt
    18721 tgaagaccat aagattgtgg cgcgtgcggc tcgtcaaggg gaaagtttag tcacacttga
    18781 tggtgaaaaa cgggaattga ccactgagga tcttgttatt accgtggctg acaaaccagt
    18841 tgctttagca ggtgttatgg gtggtcaagc cactgaaatt gatgccaact ctcagacggt
    18901 tgttcttgaa gccgctgttt tcgatggcaa atcaattcgt aaaacgagtg gtcgtttaaa
    18961 ccttcgttcg gaatcatctt cacgctttga aaaaggggtt aactatgcaa ctgttttaga
    19021 agcgcttgac tttgcagcgg ctatgttaca agaattagca gaaggtcaag tcttatcagg
    19081 ccatgttcaa gctggacaat tgccaacaga acctgttgaa gtctcaacta gccttgacta
    19141 tgtgaatgtt cgtttaggaa cagaattaac ttttgctgat attcaaagga tttttgacca
    19201 acttggattt ggtctaacag gagatgaaac aagcttcacg gtagctgttc ctcggcgtcg
    19261 ttgggatgtt agcatcccag ctgacttggt ggaagaaatt gcgcgtatct atagttatga
    19321 taagttgccg acaaccttac cagaggcagg gggaacggct gcagaattaa cgccaacaca
    19381 ggctcttcgt cgtaaagtcc gtggcttagc ggaaggattg ggcttgactg aaatcatttc
    19441 gtatgccttg acaacaccag agaaagctgt tgagtttgca gtggcaccaa gtcacctaac
    19501 agaactcatg tggccaatgt ctgtggaacg ttcagcgctt cgtcaaaata tggtttcagg
    19561 gatgttagat acggtagctt ataatgtggc tcgtaaacaa agtaacttgg ctctttatga
    19621 aattggaaaa atctttgaac aagaagctaa tccaaaagaa gaccttccta atgaagtgaa
    19681 ccattttgct tttgctattt gtggcttagt ggcacaaaaa gatttccaaa cccaagctca
    19741 ggcagtggat ttttaccatg ctaaaggtat cttggatact ttatttgcaa accttaacct
    19801 caaggttcaa tatgtgccaa ccaaagacct agcaaacatg cacccaggac ggacagcgct
    19861 tatcctatta gatgagcaag tgattggatt tgttggtcaa gttcatccag gaacagcaaa
    19921 ggcttatagt attccggaaa cttatgtggc agaattggat atggctgctt tagaagcagc
    19981 gttgccaagt gatcaaactt ttgtggagat taccaaattc cctgctatga cacgtgatgt
    20041 tgccttgtta cttgaccgtg aggtgagtca tcaggccatt gtaacagcta ttgaatcagt
    20101 aggtgtaaaa cgcttgacta agattaagct ctttgacgtg tatgaaggag caaccattca
    20161 ggctggcaaa aaatcaatgg cttatagcct tactttccaa aatccaaatg acaatttaac
    20221 ggatgaagaa gttgccaaat acatggagaa aatcaccaaa gcattgacag aacaagtcgg
    20281 tgctgaagta cgctaagaaa aaaactgggc ctctaaggct ccgttttttt ctgttataat
    20341 agaggaaaaa agaactccag ttcaaatgat tggagaaaaa catcagaagg tggaggaaaa
    20401 tcagaatgta tcaaacaaaa ataacaggca accgccttta ccacacggtt tcgaaaggct
    20461 acggagatag tgtaacctta tttggaaaaa ctgatcaagg ggaaacccct atgagtttac
    20521 tggttattgc cttgtcttct tgtgtgacta tgtgtgtgca gggtttttac catcgtcaat
    20581 ttcagcaaga cacacttgct gtttctgttg aggctagtta tgaggaagaa gtttttgaac
    20641 tacttgttag gattgatgat gttttagatg acaaaaagaa agctgattta ttggcttaca
    20701 ttggaactta ttgtcgtgtg aaacaactcc tcaaacctga agttacggtg accattacgt
    20761 tggaaggact gtctcatgtc tgatgctagt ttgaaaactt accaaaaaca atgggcttat
    20821 caaaagtatt gggtcatggc acactcacag cagcattaca atgctttaag agaattgttt
    20881 aaaggaaatc agtggtcaga agaaaaggtg ttgacttttc attgtttgat tgaagaagcg
    20941 caagccattc ctcccacagt aaaaagctta cgaacagcat atcagcatgt ttgggggtat
    21001 tttaaaaagg tagcgagtca ggaagaaaaa gaccatttca aagatttgga tgctcagttg
    21061 gaaactaaat ctgaggagat gctatgcttt ttacaagaga tgactgctca ttatcagccc
    21121 tcttatctgc tatcttgccg tttgattacc aaaggtcctt aaatgctaaa cagaaaagta
    21181 gggatttctg ggaagttatg ctaagctagt gatataaata agggggaatg agagatgttt
    21241 ttagcgctta atgaaatgaa acaatccaaa ttgcggtatg gtttgattgc tggtttgcta
    21301 tgtttggttg cctatttgat gttctttttg tcaggattgg cctttggttt gatgcaggag
    21361 aaccgctcgg cggttgatct ttggaaagct gacagtgttt tactagccaa ggatgctgac
    21421 gctaccttga ccttatcaca ggtgtccaga gctcaagaaa atcaaataac agcaaacaag
    21481 gtagctcctc ttgctcaact caataccgtg gcgtggtcag ttaaaaatcc taaggatgcc
    21541 gacaaagtta aggttagcct tttcgggatt gattctaata gctttattcg tcctaacatt
    21601 gtaaaaggtc gattatttaa gactaacaaa gaggttgttt tggatcaaag ccttgcaaaa
    21661 gaggaagctt ttgcgattgg caaggacttt tacacatcga gttctagtca agcattaact
    21721 atcgttggtt atactcaaaa tgctagattt agtgttgcac cagtggttta tatgaatttg
    21781 gaagcttttg aaacattaaa atatggagaa ccactaccaa aagataagca agttgttaat
    21841 gcttttatca ctaaaggaag tttaacagat tatcctaaaa aagacttcca aaaattagat
    21901 attaaaacct ttattactaa attacctggt tatagcgctc aacttttaac ttttggcttt
    21961 atgattagtt ttcttgtcat tatttcagct attattattg gtatttttat gtatattttg
    22021 actattcaaa aggcacctat ttttgggatt atgaaagcgc aaggaatttc taacaaaacg
    22081 attacgaccg ctgtgcttat gcagacattc tttttgagtt ttttaggtag tggtttaggg
    22141 ttgctaggta cttggctgac atcattatta ttaccaacag tagtaccttt tcaaagcaat
    22201 tggtttttat atttggctat attcgttagt atgatctgtt ttgctctatt aggcacctta
    22261 ttttctgttt ttaacattat acgaattgat cctttgaaag caattggata ggaggtagtg
    22321 atgtcggttt taacatttaa acaagtgacc aaaacatttc aagatggtca tcacgaaatc
    22381 aacgcattaa aggcaactga cttttccatt gaagctggag aatttgtggc tattattgga
    22441 ccgtcaggtt ctggaaaatc aacctttttg accattgctg gtggcttaca gaccccaagt
    22501 tctggtcagc taatcattga tggcactgat tatacgtatt tatcagaaaa agagcgctca
    22561 cgcttgcgct tcaaatctgt tggttttatc ttacaagctt ccaatctgat acctttttta
    22621 acggtccagc agcagttgga attggtcgat catttgacag gaagcaaaga aaaagccaaa
    22681 gccaatcagt tatttgatga tttaggcatt gcagggctaa aacatcagtt acctcaagaa
    22741 ttatccggcg gagaaaggca aagagcggct attgcccgtg ctctttacca tgatccagca
    22801 ctagtattgg cagatgaacc tactgcaagt ttggatactg aaaaagccta tgaagtggtc
    22861 aaactgttgg ctaaggaaag taaagaaaaa aacaaggcca ttattatggt cactcatgat
    22921 gatcggatgc tagaatattg tgacaaagtc tatcggatgc aagatggaga gttacgtcaa
    22981 gagagatagt agactgataa gagtttatga tattagaaga aaaaaccaaa caagcacagc
    23041 acttgtctgg tttttttcta agtgtgacta ttatcaatac gtcaccattt ccctaaagct
    23101 tactatacgg gatgttaaag gtatcaccat gctcaaagtc gccatattgg taacctcggt
    23161 caaaccaacg ttggcgttgc ttagaagttc cgtgggtaaa actatcagga acggctctgc
    23221 cataggtttc ttcttgcaga gtatcatccc caactgcgtg agctgctgcc attgcttcct
    23281 caatatctcc tttttccaaa aggccttgcc cttgaacata attagcccag gcaccagcat
    23341 agtaatcagc ttgtaattcc aacttgacgt tgagctgatt ggctttagcc tttgattttc
    23401 cttgtctagc gctagcgtaa ttatccataa ttcccaattc attttggatg tggtggccaa
    23461 cttcatgagc aatcacatag gccatagcga aatcaccttt tgcgccgtac ttggttgata
    23521 gttcattata aaaagaaata tctaaataca ctttttgatc gcctgggcaa tagaaaggac
    23581 cagaagaggc ttgccccctg ccacaagcgg tttgagttgc tccagtatat agaactaatg
    23641 ttggtttatg ataagttaac cctttctcac gaaatgtctt agtccaatag tcctcggtag
    23701 aggcgaagac cttactgaca aaagaaacct gttcttgact agccttgtca ccatttgtac
    23761 gcgttacttg gcttgattga taggcattgt tgttggtaga agaaggcttg cctcctaaaa
    23821 caccagatag gccacctcct cccataacta ggagaaggag aagaataacc agcttggttt
    23881 tccaacctcc tcgtgaaaag agtaattgaa gcaggagacc gccccctagg ccgccgcccc
    23941 caaaagaacc agacgattga ccacgtctat cttcaacctg ttgactttct ctcaaatcat
    24001 ccgttttcat aagtttctcc catctatttc ttgattttat tataaaataa ttctattaga
    24061 attgctattt ttatgatgag attataacta aaaataggaa aagcaatagg tatttgctat
    24121 aatataacaa gggtagattc agtttgtata aaggagtcat atgaaattaa tttacacaga
    24181 gatgagttac tcgatgacag agattttggt gaatgaagca cgaaaagctg ctgaccaagg
    24241 ttatcgagtg ttctatattg ctccaaattc tctttcattt gaaaaggaaa gagaagtctt
    24301 aaccttattg cctgaacgag ggactttttc gataatagtc actcggttcg ttcaaatgtc
    24361 aaggtatttt acagttgaga gctctccatc taagcaacat ctagatgaca cgactttggc
    24421 tatgattttt tatcgggcct tgatgcaatt aaaaccagaa gatttgccga gttatggtcg
    24481 tttgcaaaat aattcggttt ttattgaaca actagttgag ttatataagg aattaaaaaa
    24541 cgcacaatta agcgtacatg atttaacagg gttagaccat cctcaaaaac aagaagatct
    24601 tattaaaatt attgagcttg cagagacgat catgattcag caagattaca atcaagatag
    24661 tcctctacaa tcttttgcaa gagcaattaa gttaggactt ttaaataatc aactatcaaa
    24721 aacagttatt gttattgatg gtttttcacg tttttcagct gaagaggatt atttattatc
    24781 acttttaaat aacaactgtc aagaggtcat cattggcagt tatgtgagtc aaaaagctta
    24841 tcaaaaatct tttattaagg gaaatatcta tgaggctagc cttcattttt tgcaagattt
    24901 ggctcaaaaa taccatatca agccggtatt tgcgacctct aatcaagtgt ttaaaccagc
    24961 cttttcacgg ctaacacaat tatttgaagc aactcatgat ttttcacaag tagactggca
    25021 actccaaaag aatgatcttg atcactttag cctttggcaa tgtcatcatc aaaaagagga
    25081 aattgaacat gtagcaaaat ccattcggca aaagctatat gaaggttacc gttataagga
    25141 tatcttagtg ttgctaggag atatggatgc ttatcaatta caaattgggc ctatctttga
    25201 taagtttgaa atcccctact atttaggaaa agcagagcct atggctgccc atccattagt
    25261 ccaatttata gaatcgctcg aacgaagtca gcgctataat tggcgtcgtg aagacatttt
    25321 aaacatgtta aaatcaggac tttttggttg ttttgatgat agtgatattg atcgctttga
    25381 ggaatatact cagtttgcag atattaaagg atttactaaa ttttcaaagc cttttaccat
    25441 taactcttct aggcagtatc ctcttgactt tttaaatgaa atgagacaag acatcgtttt
    25501 acctttgcaa gaattgttta agagtcaaaa acagcttggt gcttcacttg ttgacaaatt
    25561 aatacttttt ttagaaaaga ttagattagc tgagaatatg caaggtttag cacagtccca
    25621 attagaggtg gaaaaaaacg aagaagtttg gaaaaggttt acagatattt tgacttcttt
    25681 tcatcaaatc tttggccaag aaaaattacg gctatcagat tgccttgctt taatcaaaac
    25741 gggaatgaaa tcagctcagt atcgtgttgt tccagcgaca ttagatgttg tcactattaa
    25801 atcttatgac cttgttcaac cacattcaaa acctttcgtt tatgcaattg gtctaactca
    25861 atcacatttt cctaagcaaa ttcaccattc aggactactt tctgatcatg agcgtgctag
    25921 aattaatgaa ataagaaatt atcgtcattt tgatattgct agtgccgaaa atagtaaaaa
    25981 aaaccatcaa actgcgttat ctctgtttaa tgctgctact aaggagttag ttcttagtgt
    26041 ttcaacggta atcaacgaga cattcgatga tctatctcct tatcttaaag aattaataaa
    26101 ctttgggtta cctcttttgg ataaaggcaa aaactatcta tcttatgata atagcgatat
    26161 tggtaactat aaagctctct tgtcacaaat tattgctatt aaccgacaag atcttataga
    26221 aatgtcagat caagataaga tgttttggac tgttgtctta cgttatctca ggaaacagtt
    26281 aagaaagcaa cagctagagc tacctacttc agattatcgt ttatcaacaa agcctttgtc
    26341 taaagaagtt atcgaggtat gttttccaaa gggaatccct ttaaagctat ctgcaactgc
    26401 tttaaccgtt ttttataata atcagtacaa ctattttttg aaatatgttt tgaatttaaa
    26461 caaaacagaa tccattcatc cagattccag aattcatggt caatacttgc atcgtgtctt
    26521 tgagcggtta atgaaagatc acacgcaaga accgtttgat aataagttaa aacaagcaat
    26581 ctaccatacc aatcaagaaa gtttttttca gcaggtttac caagataatg ccgaggctga
    26641 gtacagttta gctatactag aagatattgt tagatcaaca gcacctatct tgcaacttaa
    26701 ccaaaacata caggtcattg atcaagaaaa gaactttcag ctggatatgg gaaatgagat
    26761 attagtccac gggataattg atcgtattga ccagctgagc gacggtagct tgggcattgt
    26821 cgactataaa tcaagcgcga atcaatttga tattgggact ttttataatg ggttgagtcc
    26881 acaactagtg acttacctcg cagccctaaa acagatcgca ccgcacgata taaaccaatt
    26941 atttggggct atgtaccttc atttgcaaga tcctaaattg gatctagtaa catttaaaca
    27001 aattgataac acattagtgg agagtatcta taaagcattg acctataaag gtattttttc
    27061 agaagtcgaa aaagagcatc tatctactgg tacttaccaa accaaaaatg ctctttattc
    27121 caatgacgaa ttggaaacac ttttaaatta taacaagtac ctctatctta aagctgctaa
    27181 acacattaaa aagggatatt ttttgattaa tccttataca agcgatggta agacagttca
    27241 aggagatcaa ttaaaagcca ttacccgttt tgaagcagat cttgacatgg gacaggcaag
    27301 gcgactagtg actctccctg ctaaggagaa aaaagagtgc tttttaacat taatgagaaa
    27361 ggagagccac ttgtgatttc ttttgcccca tttttaagcc ccgaagctat taaacattta
    27421 caagaaaacg aaaggtacag tgatcagtct caaaaacgca cagctcaaca aattgaagca
    27481 atttatacta gtggccaaaa tatacttgta tcagcttctg ctggttcagg aaaaaccttt
    27541 gtaatggtcg aacgcatact tgataaaatt ttgagaggtg tttcaattga tcggcttttt
    27601 atctcaacct ttactgttaa agcagctaca gaactgcgtg aacggattga aaacaaatta
    27661 tactcacaaa ttgctcaaac tacagatttt caaatgaaag tttatttaac agaacaattg
    27721 caatctcttt gtcaagctga tattggtact atggatgctt ttgcacagaa agtagtaagt
    27781 cgctatggtt atagcattgg catttcatcc caatttcgta tcatgcagga taaagcagaa
    27841 caagatgttt taaagcaaga ggtgtttagc aaactcttta atgagtttat gaatcaaaaa
    27901 gaggcaccgg tgtttagggc tcttgtgaaa aatttttctg gtaattgtaa agacacttca
    27961 gcttttagag agttagttta tacttgttat tcttttagcc aatcgacaga aaatccaaaa
    28021 atatggttgc aagaaaattt tctaagcgct gctaaaactt accaaagact tgaagatatc
    28081 ccggatcatg atattgaact cttacttttg gcaatgcaag acactgcaaa tcagctaaga
    28141 gatgtgactg atatggaaga ttatgggcag ctgactaagg caggtagccg atctgctaaa
    28201 tacactaaac acttaacgat catagaaaag ttgtctgatt gggtgcgtga ttttaaatgt
    28261 ttgtatggaa aagccggatt ggatcggttg atcagagatg tgacagacct tataccatct
    28321 gggaatgatg ttacagtctc gaaggtaaaa taccctgttt ttaagacctt gcatcaaaaa
    28381 ttaaaacaat ttaggcattt agaaacaatt ttaatgtatc agaaagactg ttttccctta
    28441 ttggaacagt tacaagattt tgtgcttgcg ttttcagaag cttatttagc tgtcaaaata
    28501 caagaaagcg cttttgaatt ttcagatatt gcacactttg caatcaaaat tttagaggaa
    28561 aatacggata ttcgccaatc ctatcagcaa cactatcatg aggtgatggt tgatgaatat
    28621 caagataaca atcatatgca agagcgactc ctgaccttac tatcgaacgg tcataatcgc
    28681 tttatggtag gagatatcaa acaatcgatc tatcgatttc ggcaagccga tcctcagatt
    28741 tttaatcaaa agtttagaga ctatcaaaaa aaacctgagc aggggaaagt gattttactc
    28801 aaagaaaact ttcgtagcca atcagaggtg ttaaatgtca gcaatgctgt ttttagtcac
    28861 ttaatggacg aatcagtagg agacgtctta tacgatgagc aacaccagtt aatagcaggt
    28921 agtcatgctc aaacagtccc ctatctagac cgtcgtgctc agttattgct atataatagc
    28981 gataaagatg atggcaacgc cccttcagat agtgagggta tttcatttag tgaggttaca
    29041 attgttgcca aagaaattat taagcttcac aatgataagg gtgtcccttt tgaagacatt
    29101 acgttactcg tttcttcaag aacaagaaat gatatcattt ctcatacatt caatcaatat
    29161 ggtattccta tagtaacaga tggtgggcag caaaactatc ttaaatctgt tgaagtgatg
    29221 gttatgttag atacattacg caccattaat aacccaagaa atgattatgc ccttgtggct
    29281 ttactgcgct caccgatgtt tgcctttgat gaggatgatt tagcaagaat agcacttcaa
    29341 aaagacaatg agctagataa agattgccta tatgacaaga tacaaagggc tgtgattgga
    29401 agaggtgctc atcctgaatt gattcacgat accttgcttg gcaagttaaa tgttttttta
    29461 aagacgttga aaagctggcg tcgatacgct aagctagggt cgttgtatga cttgatttgg
    29521 aaaattttta atgatcgttt ttattttgat tttgtagcta gtcaagcaaa agcagaacaa
    29581 gcacaagcta atctatacgc attagctcta cgtgctaatc agtttgaaaa atcgggctat
    29641 aaagggctat accgttttat taaaatgatt gataaggtac ttgagacgca aaatgactta
    29701 gctgatgtgg aagtggctgc tcctaaacaa gctgttaatt taatgaccat tcacaagtct
    29761 aaaggtttac aatttccgta tgtatttatc cttaattgtg acaagcgctt ctcaatgaca
    29821 gatattcata aatcatttat tctgaatcgg cagcacggta tcggtatcaa gtaccttgca
    29881 gatatcaaag gtttacttgg tgaaacaaca ctcaattctg ttaaagtaag catggaaacc
    29941 ttaccttatc aattgaacaa acaagagttg cgcttagcaa ctttatcaga acaaatgcgc
    30001 ttactgtatg ttgctatgac acgagctgaa aaaaaagttt attttattgg taaagctagt
    30061 aagagcaaaa gtcaagaaat cacagatcct aaaaagttag gcaaactttt gccgctggct
    30121 ttacgagaac agttattgac attccaagat tggctattag caatagcaga tatgttttca
    30181 actgaagatc tttattttga tgttcgcttt attgaagata gtgatttgac acaagagtca
    30241 gtcggacgac ttcaaacacc acagttatta aatccagatg atcttaaaga taatcgtcaa
    30301 tcagaaacaa ttgcacgggc tttagatatg ttagaagcag tgtctcaatt gaatgccaat
    30361 tatgaagcag ctattcattt gccaacagtt cgaactccta gccaacttaa ggcaacttac
    30421 gagcctttat tagaacccat tggtgtagat attatagaga aatcttctcg atcgctatct
    30481 gattttactt tgccacattt ttcaaaaaaa gcaaaagttg aagcaagtca tattggatca
    30541 gctcttcatc agttgatgca ggtgctctct ttgtcaaaac cgataaatca acaaacgctt
    30601 ttagacgctt taagaggaat tgatagtaac gaagaggtaa aaacagctct tgatctcaaa
    30661 aaaatagagt cgttcttttg tgatacaagc ctaggccaat tttttcagac ttaccaaaaa
    30721 cacttgtatc gagaagcgcc atttgctatt ttaaaagttg accctattag tcaagaagaa
    30781 tatgtcctac gtggtattat agatgcctac tttttgtttg atgatcatat tgtattagtg
    30841 gactataaaa cagataaata caagcagccc attgagttaa aaaagcgtta ccaacaacag
    30901 ttggagttat atgcagaagc tctcactcaa acgtataaac ttcctgtgac taagcgctat
    30961 cttgttttaa tgggaggtgg aaagccagaa attgtcgaag tttaaggttg ttgtgacaca
    31021 agggtatttt gcaaatgata atagtatagt tgaggatttt acgaaatgct tgaaaagcta
    31081 ttgacctttg aataataata caatataata agttttatta gcgacaaaaa ggaataaata
    31141 tactatgaat tttaaaaaaa taagatttgg ttttttgctt tgcctcctac tttatccttt
    31201 agcagcttgt aacaagtctg aacaacttaa tcactacgaa agaattaaaa aaacaagaaa
    31261 attagtcgtt gctgtaagcc ctgattatgc tccttttgaa tttaaagctt tagttaatgg
    31321 aaaagatact attgttggag cagatgttca attagctcag gctattgctg atgaattaga
    31381 cgttgatctt gagctttcac caatgagttt tgataatgtt ttgtctagtt tacagactgg
    31441 taaagctgat cttgctattt caggaatctc acatactaaa gaaagagcta aagtatatga
    31501 tttttcaatt ccttactatc aggcagaaaa cgctatagtg atgagagcat ctgatgctaa
    31561 agtgaccaaa aatattagtg acctaaacgg taagaaagta gccgctcaaa aaggtagtat
    31621 cgaggaaggt ttagttaaaa tacaattacc aaaggccaat ctgatttctt tgactgctat
    31681 gggagaagcc attaatgaac taaaagcagg acaagtctat gcagttacct tagaaacacc
    31741 tgtagctgct ggttttttag cccaacataa ggatctggct ttagcaccct ttagcttaaa
    31801 aaccagtgat ggagatgcca aagcagtggc ccttcctaaa aatagtggtg acttaaccaa
    31861 agccgttaat aaggttattg ctaagttaga tgagcaagaa cggtacaagt catttattgc
    31921 agagacaata gcactaacga aaaatactat gaagtaataa ggttaataaa aactttatta
    31981 gtatccgttg gaaaagtgtt tggaataaac aataaaagag gtttgacttt tttaaaaaaa
    32041 taatgtaata taattgggaa cgtgaaaatg ttacaatatc ttgaggaggt gaaacacatg
    32101 tcaaaaacag tagtacgtaa gaatgaatct cttgacgatg ctcttcgtcg cttcaaacgt
    32161 tctgttacga aagctggtac tcttcaagaa tcacgtaaac gtgaattcta cgaaaaacct
    32221 tctgtaaaac gtaaacgtaa atcagaagca gctcgcaaac gtaaaa
//
LOCUS       DAUCWU010000012        29711 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUCWU010000012 DAUCWU010000000
VERSION     DAUCWU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29711
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.012"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            179..1048
                     /locus_tag="VGY24_000541"
     CDS             179..1048
                     /locus_tag="VGY24_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283550.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            1045..1803
                     /locus_tag="VGY24_000542"
     CDS             1045..1803
                     /locus_tag="VGY24_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283551.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            2008..3669
                     /locus_tag="VGY24_000543"
     CDS             2008..3669
                     /locus_tag="VGY24_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ4283552.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKDKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            3802..4587
                     /locus_tag="VGY24_000544"
     CDS             3802..4587
                     /locus_tag="VGY24_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ4283553.1"
                     /translation="MANIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFEDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            4617..4943
                     /locus_tag="VGY24_000545"
     CDS             4617..4943
                     /locus_tag="VGY24_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283554.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            4989..6896
                     /locus_tag="VGY24_000546"
     CDS             4989..6896
                     /locus_tag="VGY24_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ4283555.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLMVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQEALME
                     EWETIAEQIES"
     gene            7181..8149
                     /locus_tag="VGY24_000547"
     CDS             7181..8149
                     /locus_tag="VGY24_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ4283556.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            8208..9206
                     /locus_tag="VGY24_000548"
     CDS             8208..9206
                     /locus_tag="VGY24_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ4283557.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAATTGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            9391..10800
                     /locus_tag="VGY24_000549"
     CDS             9391..10800
                     /locus_tag="VGY24_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ4283558.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            11127..12890
                     /gene="lpdA"
                     /locus_tag="VGY24_000550"
     CDS             11127..12890
                     /gene="lpdA"
                     /locus_tag="VGY24_000550"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ4283559.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGCNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAM
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(13564..15981)
                     /gene="hylA"
                     /locus_tag="VGY24_000551"
     CDS             complement(13564..15981)
                     /gene="hylA"
                     /locus_tag="VGY24_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ4283560.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASTNITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEASSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTFVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQEGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATTFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            16214..17203
                     /locus_tag="VGY24_000552"
     CDS             16214..17203
                     /locus_tag="VGY24_000552"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ4283561.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGTVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILAKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(17312..18103)
                     /locus_tag="VGY24_000553"
     CDS             complement(17312..18103)
                     /locus_tag="VGY24_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ4283562.1"
                     /translation="MTYTSLQSPENQDYIYDLNIAHLYGNLMNTYGDNGNILMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGGEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(18103..19446)
                     /locus_tag="VGY24_000554"
     CDS             complement(18103..19446)
                     /locus_tag="VGY24_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ4283563.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     ERQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFVCLNCQFQRPTLDYQLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIIKTIEKQEAKHAYILATYTAMLEFRSLLADRHVVEKEMI"
     gene            19553..20404
                     /locus_tag="VGY24_000555"
     CDS             19553..20404
                     /locus_tag="VGY24_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ4283564.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVTLPWYKK
                     ILGGKSK"
     gene            20401..21357
                     /locus_tag="VGY24_000556"
     CDS             20401..21357
                     /locus_tag="VGY24_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ4283565.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVDKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            21411..22766
                     /locus_tag="VGY24_000557"
     CDS             21411..22766
                     /locus_tag="VGY24_000557"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ4283566.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGILVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKERAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            22900..23541
                     /locus_tag="VGY24_000558"
     CDS             22900..23541
                     /locus_tag="VGY24_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283567.1"
                     /translation="MTKQQIEKLADLLHPFAATLYLDMIPEFQEGYASYVYQTQKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            23604..24734
                     /locus_tag="VGY24_000559"
     CDS             23604..24734
                     /locus_tag="VGY24_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ4283568.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKAAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKKEMMEEELFLGLRKKTGVSIQRFQEKFGISFEE
                     CYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            24744..25496
                     /locus_tag="VGY24_000560"
     CDS             24744..25496
                     /locus_tag="VGY24_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ4283569.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGC
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFETDFVKKLHRVPKMPLLEQSIDRYYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESEID"
     gene            25496..26260
                     /locus_tag="VGY24_000561"
     CDS             25496..26260
                     /locus_tag="VGY24_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ4283570.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLTNQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            26260..26892
                     /locus_tag="VGY24_000562"
     CDS             26260..26892
                     /locus_tag="VGY24_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ4283571.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
     gene            27160..27620
                     /locus_tag="VGY24_000563"
                     /pseudo
     CDS             27160..27620
                     /locus_tag="VGY24_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            27690..29522
                     /gene="lepA"
                     /locus_tag="VGY24_000564"
     CDS             27690..29522
                     /gene="lepA"
                     /locus_tag="VGY24_000564"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ4283572.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDTKK"
BASE COUNT         9171 a         5173 c         6346 g         9021 t
ORIGIN      
        1 tgagtaagtt taaacatgat tctcgacttt tttgttttct gtcttagttt cgtcaaagtg
       61 attgaaaaaa agactaagcc acggtgctga actaagtata aaacaaatct aaaaattaat
      121 gtaaagaaag ggtttatact ggcgagagtt acccgctaat ataaatatga ggaaaaatat
      181 gattatttca tcagtttcac aaggccttat atggggagtt ttaggattag gaatctacct
      241 gacctttagg attttaaact tccctgacat gactacagaa ggttcttttc ctttaggagg
      301 agctgttgcg gtcaccgcta ttagcttagg gtggaatcca tttctatcaa ccttacttgg
      361 tatgttatca ggtgctttag caggtttttt aacagggctg ttatatacga aaggaaaaat
      421 gccaacactt ttagctggaa ttttggtgat gacgtcatgt aactccatta tgctaatggt
      481 aatgggaaga gcaaacttgg gtttacatga tcataaacgt attcaggatt gtttaccttt
      541 ttcaattgat ttaaatagcc ttttaacagg tctgataact gttgtgattg ttattagcgt
      601 tttgatctat ttcctttaca ctaatctagg tcaagcttat atcgctacgg gagataataa
      661 agatatggct aagagttttg gtattaacac ggattggatg gaagttatgg gacttgtggt
      721 ttctaatagt ttaatagctt tatcaggtgc tctagtgagt caacaagatg gctatgcaga
      781 tgtctcaaaa gggattggag ttattgttat tggtttggcg agcataattg ttggtgaagt
      841 actatactca actggactga ctcttttaga acgtttgatt gctattgtaa taggctcgat
      901 tctttatcaa tttttaattt cagtagttat tacgctaggt tttaatacaa gttatttgaa
      961 attaattagt gctcttgttt tagcactgtg cctcatgata ccagtggtaa aagagcgctt
     1021 ctttaaggga gtgaggttaa cacgatgaca aaaattattg aattgatcaa tgcaacagtt
     1081 gacgtagata atggttttga agatgctaaa acaatattgg acaatgtgac actaactatc
     1141 tatgaacatg attttttaac tattttagga ggaaatggag ctggaaaatc aacccttttt
     1201 aatgtaattg caggaacgct aagtttgaca cgggggcaaa ttaggattct tggtcaggat
     1261 gtgacgcatt ggcctgcaga aaaaagagct ctttacctat cgcgagtctt tcaagatccc
     1321 aaaatgggaa cagcccctcg gatgacagtg gctgaaaatc tcttaatcgc acgtcaaaga
     1381 ggagaaaaac gctccttggc ttctcgaaaa ataacagagc acttagctag ttttgaggat
     1441 ttggttaaac gaacaggaaa tggcctagaa aagcatttag aaacaccagc aggtcttttg
     1501 tctggtggac aaagacaggc tttaagttta ttaatggcaa cgttgaaaaa gccagctctc
     1561 ttattattag atgaacatac ggctgctctt gaccctaaga cgagtcaatc tttaatgcaa
     1621 ttgacagatg agtttgtgac aaaagatggc ttaacagctt tgatgattac ccaccatatg
     1681 gaagatgcat taacttatgg gaatcgtctg attgtaatga aagatggaaa cattattaaa
     1741 gatttaaatc aaatggaaaa agaacaactg acgattactg actattacca actatttgat
     1801 taatcaacga tctaaatcat tgacgaataa taagtcagtg atttttttga tttccgtact
     1861 gaaaataatc tgaaagtatc aaggctattt agagaaaaaa cgtcccaaat atgatatact
     1921 atagatgata gtagcatagc tacataacag taattcgact tacgttgtaa ggaccgtttg
     1981 taataataaa atatttaaag gagtatcatg actgatatta aaatgattgc cttgggaggg
     2041 gtccgtgaat acggaaaaaa cttctatttg gtagaaatta acgattctat gtttatttta
     2101 gacgcagggc tcaagtaccc tgaaaatgag caacttgggg tagatttagt catccccaac
     2161 ttagattatg taattgaaaa caaagataaa gtacaaggga ttttcttgag tcatggccat
     2221 gctgacgcca ttggagccct tccttatttg ttagctgaag tatcagctcc tgtatttgga
     2281 tctgaattga ctattgaatt agccaagcta tttgtgaaaa gtaataacag cactaagaaa
     2341 tttaataatt ttcatgtggt agacagtgat actgaaattg aatttaaaga tggcctagtt
     2401 tcgttcttta gaacaacgca ttcaatccca gaaagtatgg gtattgttat tggaacagat
     2461 aaaggaaata ttgtttatac cggtgacttc aaatttgatc aagcagcaag agaaggttat
     2521 cagacggatt tgttacgttt agctgagatt gggaaagaag gggttctagc tcttttatca
     2581 gattcagtca atgcgacaag caacgatcag attgccagcg aatctgaagt tggtgaagaa
     2641 atggacagtg ttatctctga tgctgatggc cgtgtcattg tagctgcagt agcctcaaat
     2701 cttgttcgga ttcagcaagt atttgattcc gccactgccc atgggcgtcg tgttgtttta
     2761 accggaacag atgcagaaaa tattgtcaga acagctttac gtctagaaaa attaatgatt
     2821 acggatgagc gtcttttaat taaacctaag gatatgtcta aattcgaaga ccatgaatta
     2881 atcatccttg aagctggtcg tatgggtgag ccaattaata gcttacaaaa aatggcagcg
     2941 ggacgtcacc gttatgttca aatcaaagaa ggagaccttg tttatattgt aactacacca
     3001 agtacggcta aagaagctat ggtggcgcgt gttgaaaact tgatttacaa ggccggtggt
     3061 tctgttaaat taatcactca aaacttacgc gtgtcaggcc atgctaatgg gcgtgatttg
     3121 caactattga tgaatttgtt aaaaccccag tatctttttc cagtacaagg agaatatcgt
     3181 gatttagctg cccatgcaaa gctagctgaa gaagttggta tttttccaga gaatattcat
     3241 attctgaagc gtggcgatat catggtgctt aatgacgaag gttttctcca tgaaggaggt
     3301 gtacctgcca gtgatgtcat gattgacggt aatgctatcg gtgatgtggg taatatcgtt
     3361 ttacgagacc gtaaagtttt gtcagaagat ggtattttca ttgttgctat tactgtttct
     3421 aaaaaagaaa aacgcattat ttctaaggct aaagttaata cgcgtggatt tgtgtatgtc
     3481 aagaaaagtc atgatatttt aagagaaagt gcagaattag ttaatacaac tgttggcaat
     3541 tacttaaaaa aagatacctt tgactggggc gaattaaaag ggaatgtcag agatgatttg
     3601 tctaaattct tatttgaaca aaccaaaagg cgcccagcga ttctgccggt tgtgatggaa
     3661 gttcgttagt gaaaatagga aggttaggca aaaactgcct accttctttg attttaacaa
     3721 ctggtagacc aggaaactgt tcacccaaaa atacagtaga tttacgtgat aggattattt
     3781 tggtataaag gagtgtaatg aatggcaaat attgcaattg aataccattc ggtagtttta
     3841 ggaatggagc gtaaggtaaa tgtgatttat cctgaccagt ctgaaatccc taaaaaagat
     3901 caaggggata aggatattcc tgttttatat ttgttacatg gtatgggagg caatgaaaac
     3961 tcttggcaaa aacgaacagc tattgaacgc ttgttgcggc ataccaattt gatagtcgtt
     4021 atgccttcga cagacctagg ttggtataca gacacggctt atggacttaa ctattatcgg
     4081 gcgttgtcac aagagttacc gcaggtctta gcagctttct ttcctaatat gacccagaaa
     4141 cgagaaaaaa cctttgttgc tgggctctct atgggtggct atggtgcctt taagtgggca
     4201 ctcaaaagca atcggttttc ttatgcagca tcgttttcag gtgctttgga tttttctcct
     4261 gagaccttgc ttgagggaaa cctgggagaa ttagcctatt ggcagggcgt ttttggccag
     4321 tttgaggacc ctgaccttga taagcattac cttaaaaata tggtggcaga aagtgatgga
     4381 aaaaccaaat tttatgcttg gtgtggctat gaggattttc tttttgccac taatgaaaaa
     4441 gctattgctg attttcaggc tcaaggtctt gacattgatt atcataaagg tcatggtaaa
     4501 catgagtggt actattggaa ccagcaatta gaagtcctgt tagagtggtt accgataaac
     4561 tatcaaaaag aagaaaggtt gtcttaacca tttcctagta caacatgagg tgacaaatga
     4621 aacaattact ggcgatcatt ttgtggctgc ctaagctaat agtgaaaatg ttttggcatc
     4681 ttatcaaagg gtttttacaa actatcttat tagtaacgat tattattata gggctgatgt
     4741 attattctaa tcacagtgat tccgttttag ccaataagat ctcgatagtt actgagcaag
     4801 tggtgcaaat ttttgatatc ttaacacaaa agccatctgc aaagacgcgt catgggagtg
     4861 gtaatagtca ttccactttc cttgttagcg ctaaagaagg taatcaacga cttaaaaaga
     4921 gttcgatttc ttttggaaac taggctgaaa atgtcctcaa aagggcattt ttttgttaga
     4981 atgaaagtat gattatttta caaggaaaca agttagaacg ctctttctca ggagatgttc
     5041 tttttcaaaa catatcactg caagttgatg aaagagacag aattgcatta gtagggccaa
     5101 atggagctgg taagtcaacc ttactcaagc tattggttgg ggaagaaacc ccaacgagcg
     5161 gagaagttaa tactaaaaaa gacctaacct tgtcttatct ggctcaaaat agccgttttg
     5221 aatcagatca aacaatatat gaggaaatgc tcaaggtttt cgaagcatta cgccaagatg
     5281 aaaagcgcct gcgtcagatg gaaatggata tggcaaccgt ttcaggtcaa gatttaaccc
     5341 gactgatgac agattatgat ctgcttacag agcattttcg ccagcaaggc ggctttacgt
     5401 atgaatctga catcaaagcc attttaaatg ggtttaagtt tgatgagagc atgtggcaga
     5461 tgactatcgc tgagctgtca gggggtcaaa atactcgtct ggccttggct aaaatgcttt
     5521 tagaaaagcc agaactattg gtccttgatg agccaacaaa ccacttagat attgaaacca
     5581 ttgcctggtt agagaactac ttggccaatt atcaaggagc tctaatcatt gtgagtcacg
     5641 accgttattt tttggataag gtggcaacag tgaccttgga tttaactcct catggattgg
     5701 atcgctacgt tggcaattat tctcgtttta tgaccttaaa agctgaaaaa ttagtagccg
     5761 aagaaaagca gtttgacaaa cagcaaaaag aaattgccaa gttagaagat tttgttcaaa
     5821 aaaatattgt cagggcttca actacaaaac gtgctcaggc gagacgtaag caattggaaa
     5881 aaatagaacg cttagacaaa ccgactagtg ctagaaaatc agctcacatg acttttcagg
     5941 cagataagcc gtctggcaat gtggtgttga tggttgaaaa agctgctatt ggttataacc
     6001 aacatgtctt atctgagcct attaacctag acgttcacaa attagatgcg attgctattg
     6061 ttggtccaaa tggtattggt aaatccactc tgattaaatc tgttattgga cagattcctt
     6121 tcatcaaagg agaagtgaaa tacggtgcca atgttgaaat aggttactat gaccaaaccc
     6181 aatcacattt gacgtctagc aacacagttt tggaagaatt atggcaggat ttttcaacta
     6241 cgccagaagt cgatatcaga aaccgattag gggcttttct cttttctgga gatgatgtca
     6301 aaaaatcggt ggccatgctg tcaggtggag aaaaagcccg tctgcttctt gcgaagttat
     6361 ctatggaaaa caataatttt cttgttctgg acgagccgac taaccaccta gacattgaca
     6421 gtaaagaagt gctagagaat gccctcattg actttgacgg gaccttgctt tttgtcagcc
     6481 acgaccgcta tttcattaat cgtttggcaa ctaaggtttt ggaaattacc gaaaatggct
     6541 caacccttta cttaggtgac tatgattatt accttgagaa aaaggcagag ctagaagaat
     6601 tagcccgcct agcagcagga gagacagttg aggaaacaaa agaagcctct gccactgatt
     6661 atcagctaca aaaagctaat caaaaagagc gtcgtcgctt gacccgccga tatgaggaga
     6721 ttgaagcaag acttgaaacc attgaagagc gtattggtgc tattcaagaa gacatgcatg
     6781 cctccaatga tacggcgcag ttaatcgctt ggcaaaagga atgggaccaa ttggatcaag
     6841 aacaagaagc tcttatggaa gaatgggaaa ccatcgctga acaaatagaa tcataaaaat
     6901 aagctttagc agttttgcta agcttatttt tatcgctttg ctacttgttt gaaggtgtga
     6961 aagaggtttt aaaatgatgt gttttttatg tcaaggtcgg taaaacgtga aactgttata
     7021 tagtattttt aaatttttta gtacagtttt gtttgtaatt tcacaaacta ttgcatttct
     7081 aaaaaaccaa ataaggtaag gtagaaagcg atttaattat ttgcaaattg tgaaaaaaag
     7141 ttttatcata aaaaggtaat atgataatat ggaggccgaa atggtaacag tttctaaaga
     7201 acaacatttg gatatgttcc ttaaaatgga acgtattcgt gaatttgatt cacgtattaa
     7261 taaactcgtc cgtcgtggat ttgtacaagg gatgactcac ttctcagttg gtgaagaagc
     7321 agccaatgtt ggagcggtgg ctcacttgtc ttacgatgac attatttttt caaatcaccg
     7381 tggacatgga caatcaatcg ctaaagacat ggaccttaat aaaatgatgg ctgagcttgc
     7441 tggtaaagca actggtgtgt ctaaagggcg tggtggctca atgcacttgg ctgattttga
     7501 aaaaggaaac tatggtacca atggtatcgt tggtggtggt tacgctcttg cagttggtgc
     7561 tgccctcact caacaataca aaggaactaa caatatagct gttgctttct caggtgatgg
     7621 cgctactaac gaaggatcat tccacgagtc tgttaatatg gctgcaacat ggaaacttcc
     7681 tgttattttc ttcatcatca acaaccgtta tggaatttca atgagcatca ataatgcaac
     7741 aaatacacca cacttataca cacgtgcaga agcttacgga attcctggtt tctactgtga
     7801 agatggcaat gacgtgatgg ctgtctatga aacaatgggc aaagccgttg aacatgttcg
     7861 tggtggaaat ggtccagcta ttgttgaggt agaatcatac cgttggtttg gtcactcgac
     7921 tgcagatgct ggtaaatacc gtactaaaga agaagtggat gagtggaaag aaaaagatcc
     7981 aatgatcaaa tatcgtactt atctgacaag cgaaggcatt gctactgatg acgaattgga
     8041 tgctattcaa gcacaagtca aaaaagaagt tgacgatgct tatgaatttg cacaaaacag
     8101 tccagatcca gaactttcag tagccttcga agacgtttgg gttgactaag atcaccttgt
     8161 cagtaagaag caatcgtatt tgaataataa atggagaaat aaaaatgatg tcagaaacaa
     8221 aattaatggc tttgcgtgaa gcagtcaacc ttgctatgac tgaggaaatg cgcaaggacg
     8281 aaaacatttt ccttatggga gaagacgtag gggtttacgg aggagacttt gggacttctg
     8341 ttggaatgat tgaagaattt ggtcctaaac gtgttaaaga taccccaatt tcagaagctg
     8401 ctatctctgg tgcggctatt ggtgcagcta ctacaggact acgtccaatt gtcgacgtta
     8461 ccttcatgga tttccttacc atcatgatgg acgctatcgt gaataatggt gctaaaaata
     8521 attacatgtt cggtggaggc cttattaccc ctgtaacctt ccgtgtggca tcaggttctg
     8581 gtattggttc cgctgcgcaa cactcacaat ctcttgaggc atggttgact cacattccag
     8641 gaatcaaagt tgttgctcct ggtaatgcca acgatgctaa aggcttatta aaatcagcta
     8701 tccgtgataa taacattgtt cttttcatgg aaccaaaagc gctttatggt aaaaaagaag
     8761 aagttaacca agatcctgac ttctatatcc cacttggcaa aggtgacatc aaacgtgaag
     8821 gaactgattt gacaattgtg tcatatggtc gtatgttgga gcgtgtcctt caagcggctg
     8881 aagaagttgc agcggatggt attaacgttg aagttgttga cccacgtacc ctcatcccac
     8941 ttgataaaga gttgattatt gaatctgtta agaaaacagg taaattgatg cttgtcaacg
     9001 atgcctacaa gaccggtgga ttcattgggg aaattgcaac catgattacc gaaagtgaag
     9061 cctttgatta ccttgatcat ccaatcgttc gtcttgcaag tgaagatgtt ccagtacctt
     9121 atgcacgtgt acttgaacaa gctatcttac cagacgttga gaaaatcaaa gctgccattg
     9181 tgaaaatggc aaacaaaggc aactagaaga taaggcaagt ggtctgataa aggctatgtt
     9241 tatcagtaac gtctcagcta aaaactattt tgaaagtttt tagaagaact gagttgtctt
     9301 atcccgtgtc gcggcacctc acctttcgta tgttttcgta gcaaggtgag gatgttgatg
     9361 gcgcttatca aatcgtgaaa ggatgtagaa atggcatttg aaattattat gccaaagctg
     9421 ggcgttgaca tgcaagaagg cgagatcatc gagtggaaaa aacaagaagg tgataccgtc
     9481 aatgaaggtg atatccttct tgaaatcatg tcagacaaaa ccaatatgga acttgaggca
     9541 gaagactcag gtgtgctatt gaaaattgtc cgtccagctg gtgatactgt acctgtaact
     9601 gaagttatcg gttacattgg tgcagagggt gaatcagtag atactattgc ttcaagtgaa
     9661 aaaacaacag aaattccagt tcctgcttca gcagacgctg gaccagcagt tgctccaaaa
     9721 gaaaatgtgg caagtccagc tcctcaagtg gcagcgacag ctattccgca aggaaatggt
     9781 ggaaaagttc gtgctactcc agctgcacgt aaggcagcag cagaaatggg cattgacctt
     9841 ggccaagttc caggaacagg accaaaagga cgtgtccata aagaagacgt tgaaaacttt
     9901 aaaggtgctc aaccgaaagc ttcaccactt gctcgtaaaa ttgcagcaga caaaggtatt
     9961 gatttggcaa ccgtatcagg tactggcttt aacggtaagg tcatgaaaga agatatcatg
    10021 gctattcttg aagcggctaa accagctgaa gcaaaagccc cagcagctaa agaagaaaaa
    10081 gttgttgatc ttccagaagg tgtggaacac aagccaatgt cagctatgcg taaggcaatc
    10141 tctaaaggta tgacaaactc ttaccttact gctcctacct ttacgcttaa ctacgatatt
    10201 gacatgacag agatgattgc ccttcgtaag aagttaattg acccaatcat ggctaaaaca
    10261 ggccttaaag tcagcttcac agacttgatt ggtatggcag tggttaaaac cttgatgaaa
    10321 cctgagcatg agtacatgaa tgcttccctc atcaatgatg ctaatgatat tgaattgcac
    10381 cgctttgtga accttggtat tgccgtaggt cttgatgatg gtcttatcgt tcctgttatc
    10441 catggggctg acaagatgag cttgtcagat tttgtgcttg cttcaaaaga tgtgattaaa
    10501 aaagcgcaaa ctggtaagtt gaaggcagct gaaatgtctg gatcaacctt ctctatcaca
    10561 aacttgggaa tgtttggtac gaagacattt aacccaatta ttaaccaacc aaactcagca
    10621 attcttggtg ttggagcaac tatcccaact ccaacggttg tggatggtga gattgttgcc
    10681 cgtccaatta tggcgatgtg cctaaccatc gaccaccgtt tagttgatgg tatgaacggt
    10741 gcaaaattca tggttgatct caagaaattg atggaaaatc catttgaatt gttaatctga
    10801 tcaatcagtt ttaaacaaga aaagcattca ataataaaag ggattttgct aagtttagac
    10861 ttggtgccct tttctcaaaa atatagaaag gatgtagaac gaggagctct catgcaaatg
    10921 agtgataagc aattgaaccc gagcgaaaag cccggataaa aatggtaaga gttggctaat
    10981 cttagcgtcg tagacgtctg aactttcctc aatttttgtt tcggaaagtc ctagtcgtcc
    11041 tgacgtgggt gcgtcaacga aatcaaagat ttctgactta ctgcaaaaat tcaatcgctt
    11101 tctccttgct cttggtatct taaattatgg ctgttgaaat tattatgcca aaactcggtg
    11161 ttgacatgca agaaggtgaa atcatcgagt ggaaaaaaca agaaggtgat actgtcaatg
    11221 aaggcgatat tcttcttgaa atcatgtcag ataaaaccaa tatggaactt gaggcagaag
    11281 actcaggtgt tcttcttaaa attacacgtc aagcaggtga aacagtacct gtaacagaag
    11341 ttatcggata tatcggtgct gaaggtgaat ctgttgaggt ttctagccca gctgcttcag
    11401 atgtgaatgt tgctcgtaca acagaagatt tagaagctgc tggacttgaa gtgccaaaag
    11461 caccagctca agctgcttca gctgcaccaa aagctgcact tgctgatgat gagtatgaca
    11521 tcatcgttgt tggtggtggc cctgctggtt attatgcagc tattcgtggt gctcaacttg
    11581 gtggtaaaat tgccatcgtt gagaaatctg aatttggtgg aacttgcttg aacgtagggt
    11641 gtatcccaac caaaacttac cttaaaaatg ctgaaatcct tgatggtatt aagattgcag
    11701 caggacgtgg gattaacctt gcttcaacta actataccat tgacatggac aaaacagttg
    11761 actttaaaaa caccgttgtt aaaaccttaa caggcggcgt tcaaggtctt ctaaaagcta
    11821 ataaagtcac tatctttaat gggcttggtc aagtcaaccc tgacaagact gtaaccattg
    11881 ggtcacaaac cattaagggt tgcaacgtta tccttgctac aggttctaaa gtatcacgta
    11941 tcaatatccc aggtattgac tctaaacttg tcttaacgtc tgacgatatc cttgaccttc
    12001 gtgaaatgcc aaaatcacta gcagttatgg gcggtggtgt tgttggtatc gagcttggac
    12061 ttgtttgggc atcttacggt gttgatgtta ccgttattga aatggctgac cgtattatcc
    12121 cagctatgga taaggaagtc tctcttgaac ttcaaaaaat cctttctaag aaaggcatga
    12181 agatcaaaac atctgttggt gtctctgaaa ttgttgaagc aaataaccaa ttgaccttga
    12241 aacttaacaa tggtgaagaa gttgtcgctg aaaaagctct tctttctatc ggtcgtgtac
    12301 cacaaatgaa cggtctggaa aatcttaacc ttgaaatgga tcgtaaccgt atcaaagtta
    12361 atgactacca agagacatca attccaggta tctatgcacc aggtgacgtt aacggaacta
    12421 aaatgcttgc tcatgctgct taccgtatgg gtgaagtggc tgcagaaaat gctatgcatg
    12481 gcaacacaac tcgtaaagct aaccttaaat acacaccagc ggctgtttac acacaccctg
    12541 aagtggcaat ggttggttta actgaagaac aagcgcgtga gcaatatggc gatgtgctta
    12601 tcggaaaaaa tagctttacc ggtaacggac gcgcgattgc ttcaaatgaa gcccatggtt
    12661 ttgtcaaagt tattgctgat gccaaatacc acgaaatctt aggtgttcat attattggtc
    12721 cagctgcagc agaaatgatt aacgaagcag ctactattat ggaatctgaa ttaaccgttg
    12781 atgaattgtt attatcaatt catggacacc caactttctc tgaagtgatg tacgaagcct
    12841 ttgcagatgt gcttggcgaa gctatccata acccaccaaa acgtaagtaa aacgtctttg
    12901 tcttattaat taatgaaaaa tatcatcaca taatagatca caaaaaagac tagcacaact
    12961 atgaactaca ccccaaacgt tagataaaaa aatctaacgt ttggggtgtt tttgtatgac
    13021 attaagctat gaagataagc tagacattta gcgctaaaaa aaccgagaga actccgtttg
    13081 aaagaagcat ctctgaaaag aagaagacaa aaaaccatta aaagaatgga caaagacgag
    13141 ctctgattag attttctttt aaggacggct aaagtgactc gttctaccta ctattatcat
    13201 cgattattgc accaataacc gtatcaaaat aaaacgaaaa ggacttagtc ctctacaata
    13261 cagaactaaa tcctttactt aaaatagagt agccaacttt tggggctcag tgcagtttcc
    13321 agtttttttg tctttaataa cgtttcattg ttaaccctta ctttccaata acagctaaat
    13381 ccttaagtct ttcttacgaa agggttttgt gatatggtta aaagaagcaa ctaaagctcc
    13441 acctaagaat tctaaagagg aggactgata cccagtatgc ggtaagcgtc gttgtctttt
    13501 gtggggaatt ttatgagagt taagagtatt gtttttagct gcaattcctt gatgagcaag
    13561 cttttattgc tgattttgct tgacaggatg aggaaaacta taaaagcctt gatgagaaag
    13621 attatgattt gaaaacgtcg ttgctttttt ttcatagtga atgacgtgca tttgttgact
    13681 atcatgatcg tagacagcgg ccagtttgtc attgttttct aacaagtcga tatcaagctt
    13741 gctaatatag gtttcaaatt cttgacgagt catatttgga agcatcatat aagcataacg
    13801 atcgccctct tgagtatggt tttgcatgat agtgataaag gtatttgaaa cttctttgat
    13861 ggcttctggt gatttgtcat cagcttttat gttttgccat ttccctgttt gaagtgcctt
    13921 acttatgcta agtgttgttg gcttgaagaa gtagtaacca atattttgag cgggatcatc
    13981 actttcaagg aaaatacttt tagtgtttgt aaaatcaact agctgattat tcaagtcaac
    14041 gggttgattg ttaacataag aacagtaagg gtgcttttga ttttcttttc gttgttcaat
    14101 agttgtatac gccttgtgag atgattggtt tttgatattg ctaccaacaa agattatttt
    14161 gtttcctaag ataaaccacc ctttattgag agtgaggctt ttattccaat tagtgaaggt
    14221 catagcagct agagcacttg tgttattaag ttttttactt gcaacaaaag cgtcatcaga
    14281 gagactagtc atgccaactt gttgatagtt cgttttaata ttctcaggag ttccctctag
    14341 tggtttttgc tcagtttctg tggttccagg taagcggtag ggatttaccg ttgcccaata
    14401 gttttcactg tagtgtccta aatcgttatt gtataggtaa aacattccat cagaagtaaa
    14461 ccagccatga agattttcat tattcatagc ttcataattt tgagttcgat tcgaaaacat
    14521 tgataggcca aaagcaaaat cgtgtttatt attatatagt gccaatttat ccatactatt
    14581 gaaactagct acgtaactat caagtttttg gactggaaca aaagtatcac ttagtagttc
    14641 tttcataagt ttgatatcgt gataggtttt caaattatca tagacattgt aaaaagcatt
    14701 cccttgtgtg acgagtgttt ttattcgtgt tttaagtgcc aaacggtgag gctcttcaga
    14761 catgtcagca atacgtaaaa tagcacgaag tgcttcaatg ccagcaacat gagattgggc
    14821 attaaaacga ctgatagaac gccctcgagt catatccatc atttctccac gaacgatgat
    14881 agggaaaaaa gaatggttaa tccaatgata gatagtagcc attttatccg cttctatagg
    14941 agactttgtt ttttgaataa taggaattaa ttgcgataag ccatctataa gcacattacc
    15001 gtaagctcca gtgtaagcaa tgcctttttt ataaagtgga ctttgagcgt tagtaaccac
    15061 gtgatcaatt aaagaaccgt cttggtaaaa accatttcct tcgtcaacta gcgtgaaaac
    15121 tttctcaatt gctttgattg tatcactaat ttcgagatca tctttacgaa gaataccgga
    15181 aatgagttta acacgtccca tatcaattaa atttccgcta ctggcttcaa aaggtgaaaa
    15241 attggcagcg cgaaccctaa aacgagtagg gtcaggcaca aatttttcga ttggagctgt
    15301 gtatttaaga atttcttctt gagtaaaata aggatacatc aaggataagg tattattaat
    15361 agcacgagga gttccaattt cataaaccca ccaattttct ttgttttctt ttccagttgt
    15421 ttgatgattt tcacgatcta gattataagc gtgttcatac atgaaggcca taccgtcttt
    15481 tacaatagca atagctttac tatcttgata atagcatgat tcaggattag tgatctgttt
    15541 tgctattttt tcaatattgc ggtaagtttt cgtgatatta gtagaagcgg aataatcctt
    15601 tgcatgttcc caaaggtaag ttctattttc gtgatcaggc ccttgatagc ttttaataat
    15661 gttttgagca tccttttcag ctttattatg aaaagtgacc atgtcaggat tggttttatc
    15721 ataagcatcg ttgcctgcga taatactgtt ccattggtct aacaattcag tatagtagtc
    15781 tttttgttgt ggctgtacta cctttttttc gctatctgta gtagtgagcg tttgcgtttg
    15841 aaagtaagta ttattaggtt ctgaatttga agtcagtgta tcggcataaa tggcagttcc
    15901 ttggcccatc atagcaaagc taaggaatag gtttgaataa agtagtaatt gtttatggtg
    15961 tgtgcaaaaa taagtattca ctgataaatt cctccaatat aaaaatgaga taataaaagt
    16021 agattggttt actaagtaaa aatataccac gttaaagtat ttttataaac ccttacataa
    16081 ataatggctg agtttttgtg cactaataag tgtaggtact gaagatatca tacagaatag
    16141 gacacttgtc ttttatattt gttttcatta tactagagag gtagattaca tttttgaaag
    16201 gattttttct cctatgaaat acatcgttaa caaaagtcac aatcctgcct ttaacatcgc
    16261 tttggaagcc tatgctttta gagaactagt ggaagaagac gaattgttta tcctttggat
    16321 caatgagcca gctattatta ttggcaaaca tcaaaacacc attcaagaaa tcaataaaga
    16381 atacattgat gaacatggta ttcatgttgt gcgacgttta tctggtggtg gtacggttta
    16441 tcatgatttg aataacttga actacactat catttcaaat aaaacagcgg aaggcgcatt
    16501 tgatttcaaa accttctcac aacctgtgat tgcgacttta gctgatttgg gtgtaacagc
    16561 caactttaca ggccgtaatg atattgaaat tgatggtaaa aaaatctgcg gtaatgctca
    16621 agcttactac aaaggtcgca tgatgcacca tggttgcctt ttgtttgatg tggatatgac
    16681 cgttcttggc gatgctctta aggtaagtaa ggataagatt gagtctaaag gagtgaagtc
    16741 tgttcgtgct cgcgtgacca atattttaaa tgaattacca gaaaaaatta ccgttgagga
    16801 attttcagat aaaatcttgg ccaagatgaa agaaacgtac ccagacatga cagaatatgt
    16861 cttgtcggag gatgagttgg ctaagattga acaatctgcc aaagagcaat ttggtagctg
    16921 ggattggacc tacggcaaag cgccagaata tacgattgaa cgcaatgttc gttaccctgc
    16981 aggtaaaatt tcaacctttg caaatgttga aaattctatc attaaaaacc ttaaaatcta
    17041 tggtgatttc tttggaatca aagatgtgca agacattgag aacttattaa ttggttgtaa
    17101 atatgagtac cgtgatgttt ttgaacgttt aaaaacgatt gatacgaccc agtacttctc
    17161 acgcatgacc gttgaagaag ttgctaaagc tattgtttct tagtggtttt aatcacttaa
    17221 gaagctctgt cagaagaaaa aactataaaa ctcctaagcg aactatcgtg tcctacttag
    17281 gagtttttgt ttgatgacat gaaagaagaa cttaaacttt attaaaggaa gctttgcttt
    17341 taaggtcggc atattcttca gtgatttctt gtttcagtat atcatcatag ctaggtagtg
    17401 aaatagctga tccatacttt tttttaagag ctgttgttac taagcggtaa gcaagattaa
    17461 cattgcgtga taagatagga ccgtggaaat aactaccata gacgttttta tagtgaacac
    17521 cttcagtttg gtcttcttta ttattaccgt tgccataaac cactcgtcca agtggctttt
    17581 cgccaccaga taagaaggta cgtccttgat gattttcaaa gccataatag gtttcgttaa
    17641 attcgtcatt atgaatttta atatctccaa taaagcgatt ttggtgctga ttaagggtgt
    17701 agtgtcccat aattccaagt ccatcaattt taaccccatt agcttggaca tagtattgcc
    17761 ctagtaattg gaagccacca cagatggcaa gcaccacttt attattagca atgtaatctg
    17821 ctaaggcagc ttttttgcta ggcaaatcct tggcaacaat gctttgttca taatcttgac
    17881 caccaccaaa gaaaacaata tcgtaatcgt cttgttcaaa ggtatcattg atagatacaa
    17941 tatcaacagt aaccctagct ccaagttttt cagcgacata cttgagcatt aaaatattgc
    18001 cgttatcacc ataagtattc ataagattac catagagatg ggcgatattg aggtcgtaga
    18061 tgtagtcttg attttcggga gattgtagtg aagtgtatgt cattagatca tctccttttc
    18121 aacgacatgg cggtcagcaa gtaagctacg gaattctaac attgctgtat aggttgctaa
    18181 aatataggca tgcttagctt cttgtttttc aatggttttt atgatttgct cgagcttttc
    18241 agcttgtttt atcttagtat cgtcaaatcc tgtgactcta agtcttctag caatttcaga
    18301 gtgacggacg ccgccagcat taatttctgt aatgggcatt tgggtaatga gttcaaaatt
    18361 agcatcccaa atccagctgg tatcaatgcc gtcagcgtaa ttagcattta aaagcacaga
    18421 taagctaaat gggtaatcag ctagctgaat catttcaagc gcttgactgg ctccaacagg
    18481 attcttgata aggataaggg tgcatgattt atcaccaata gtaaaagttt cttgtcttcc
    18541 aaaaaccgcc ttacttttgt taaagcccgc cttgattttc tcgggagaaa caccgaaaaa
    18601 ttcagcaaca gagacggcag caagcgcatt gtaaatatta taaagaccac caacatttat
    18661 cttgtagttt tgtccatcaa taacaaattc agaactttgg tgagtaatag cggtgagctc
    18721 agttaattga taatctaacg ttggtctttg gaactgacag tttaagcaaa caaagtctcc
    18781 tagattcgca taagtattga gacgatactg aagaatatgt tcgcattttg gacataaaat
    18841 cccttctgtg ttgtaatgag ctagctgcgg agcatgctta gcagtatcaa aaccataata
    18901 ctgaactgga ttaactatgt ccttagaaga gaaaatagga ctatctccat tggctagaat
    18961 agttgctttt ggagcatttc gagcaccatc tacaatcatc tgataagtag tataaatctc
    19021 tccgtagcgg tccatctgat ccctaaaaat attagtataa acaaaaagac taggttttaa
    19081 gtaagtggta attctaggaa gactagcctc gtcaatttct aaaacagcaa tttgcctttc
    19141 agatttccct tttttagcag ctaaaaaagt agaagtaatt ccagtaatca tattggcacc
    19201 acttggattt gtgattatct cgccaaatgc ctctttcaaa ataccgacag taagagcagt
    19261 tgttaacgtt ttaccattag taccagtgac gaccacaata tcatagtctt tagacaaatc
    19321 ctttaaaata tctttattac aagctagagc caatcgacca ggataagtag atcctcgccc
    19381 tagttttgtt aaaatggatt gagcagcttt gccagcaata atgccgagta atgtcttcat
    19441 tttcatgctt atattttatc ataagtttat tgattttaga tagggaaaaa tctttgatca
    19501 tgatatgata taatatagct aataactttt ttgatataga aatgagagtt gtatgaataa
    19561 tttatctagt atcgatatta aatttttatt aagcttattt gcggatcctt ggctgctggc
    19621 agtgcatctt ttagatattc taattgttgc ctatttgatt tatcgtttta ttaaagcatt
    19681 gactggaact aaaatcatgt ctttagttca aggggtcatt ttctttttgg ttcttcgttt
    19741 tattgcagag tggattggct ttacgacaat cacatactta atgaaccaag tcattactta
    19801 tggtgtaatt gcaggagtag tcatttttac ccctgaaatt cgggcaggac tggagaaatt
    19861 tggtcgttct acgcaagttt ttttacaaaa acagtacgtc agcagtgaat ctgctttggt
    19921 cgatgctttg attaaatctg tggcctatat gggtccacgc aagataggtg cactgattgc
    19981 tattgaacag actcaaacct tacaagaata tattgcgaca ggaattccgc tgaatgccga
    20041 tatttcaagt caactactca tcaatatttt tattccaaat acgccgcttc atgatggagc
    20101 tgttatcgtt ggtcagaata agattgtggc agcctgtgcc tacttgccct tgtcagaatc
    20161 aaaggcaatt tccaaagaat ttgggacccg tcatagagcg gctattggat tatctgaaaa
    20221 ctctgatgca ctaactatca ttgtttcaga agaaacagga gctatttcag taactcgcaa
    20281 aggccaattt ttacacgatt taagcacaga tgaatttgaa acggtactta ggacctacct
    20341 gatgagtgat tcaaatgtta ctttaccatg gtataagaaa attctaggag ggaaatctaa
    20401 atgaaacgat ttttaaatag tcgtccttgg ttaggaatgg tatcagtctt ttttgcgatt
    20461 ctcctttttt taactgcagc atcgagcaat cataataact ctagctcgca aatttatagt
    20521 ccgattgaaa cctatacgca tagccttaaa gatgtaccaa ttgatatgaa gtatgatagt
    20581 gacaaatatt ttatcagtgg gtattcttat ggtgcggaag tttatctaac ctccactaat
    20641 cgtatcaaat tagattctga agtgaacaat gatacacgta attttaagat tgtggcggat
    20701 ctaacacata gtcatccagg tacagtaaat gtcaacctaa gagtagaaaa tcttccttct
    20761 ggggtgacag ctaccgtatc tccagataaa atatcagtca caattggcaa aaaagaatct
    20821 aaggtatttc ctgttagagg aagcgttgat gctaaacaaa ttgcaaatgg ttatgaaatt
    20881 agcaaaattg aaacgggggt tgataaggtt gaagtgacca gtgatgaatc taccattgct
    20941 ttaattgatc acgttgtggc aaagttacct gatgatcaag tgttagatag gaattatagt
    21001 agtcgagtga ccttacaagc tgtctcagct gatggaacta ttttagcaag tgcaattgat
    21061 cctgcaaaaa caaatttatc agttgctgta aaaaaaataa cgaaatcagt cccgattaga
    21121 gttgaggcag tggggatgat ggatgatagc ttatcagata ttcaatacaa attgtcaaaa
    21181 caaacagctg ttatctcagg tagtcgagag gtcttagaag atattgatga aattattgca
    21241 gaagttaaca tttctgatgt cactaaaaat actagtaaga ccgtgagttt gtcttctagt
    21301 caggtgtcaa ttgagccgtc agtagtgacc gttcagttga caaccaccaa aaaataatgg
    21361 ataataacta ggaaaattga atggaggaag ccattaggct tatataaatt atgggaaaat
    21421 attttggaac agatggtgtt cgtggcgaag caaatgttga gctgacacct gagttagctt
    21481 ttaaactggg ccgattcggt ggttatgttt taagtcaaca tgagaccgaa agacctaagg
    21541 tttttgtggc gcgtgacacc cgtatttcag gagaaatgtt ggagtcagcc ttgattgctg
    21601 gtctactatc tgttggtatt gaagtttata aacttggcgt tttagcgaca cctggtgtct
    21661 cttatttagt tagaacagaa aaagcaagtg caggtgtgat gatttctgca agtcataacc
    21721 ctgcgcttga caatggcatc aaattctttg ggaatgatgg gtttaagtta gctgatgacc
    21781 aagaattgga aattgaagct ctcttagatg ctccggagga cacgcttcct cgcccgagtg
    21841 cagaaggact tggaatcttg gtagattatc cagaaggact tcgtaaatat gaaaagttct
    21901 tggtaacaac aggtactgac ttaagcggta tgacagttgc cttagacact gctaatggag
    21961 ctgcttcagt gtcagcacgt gatgtctttt tagatttaaa tgcagaaatt gctgtcattg
    22021 gcgaaaaacc aaatggtctt aatattaatg acggtgttgg ttcaacgcat ccagaacaac
    22081 ttcaagagtt agttaaggaa acaggagcag atcttggttt agcctttgat ggcgatagcg
    22141 accgtctgat tgccgtggac gaaactggtg agattgttga cggtgatcgt attatgttta
    22201 tcattggtaa atacttgtca gaaaaagggc ttcttgctca caataccatt gtcacaacgg
    22261 tgatgtctaa cctcggtttt cataaagctt tagataaaca aggtattaat aaagcaatta
    22321 cagcagttgg agaccgctat gtggttgaag aaatgcgtag ctcaggatac aatctcggtg
    22381 gtgaacaatc aggccatgtc attatcatgg actacaatac cactggagat ggtcagttga
    22441 cagctatcca attagccaag gtgatgaagg agactgggaa atcactttcg gaattagctg
    22501 ctgaagtaac catttaccct caaaaactgg ttaatattcg tgtggaaaac agcatgaaag
    22561 aacgtgctat ggaagttcca gctattgcga acatcattgc aaaaatggag gatgaaatgg
    22621 ctggtaatgg tcgtattctc gtgagaccaa gtggtacaga acctcttctt cgtgtcatgg
    22681 cagaggcgcc aacagatgcc gaagttgatt attatgtcga tactattgct gatgtcgtcc
    22741 gtacagaaat cggatgcgat aactaacgag ttgagtacaa acaagaaacc ttatttctaa
    22801 ggcttctgtc aacaattgga aagaggcttg gggcgtttag cccaagtctc tttttataaa
    22861 ttgttataaa ttgctaaagt ggaagagaaa ggatattgga tgacaaaaca gcaaatcgaa
    22921 aaactagcgg acttactgca tccttttgca gccacacttt acttagatat gattcctgaa
    22981 tttcaagaag gttatgcttc ttatgtttat caaacacaaa aacaagatat tcaaaagcgc
    23041 cgcatcaagc agttaactaa aaattttgaa cgattactag ataccccatc tttttatgtg
    23101 atgcctgtga caagaaaaac agcagagcaa ttgctagcag ataaaggcct agcttatcat
    23161 ttgttatcga cagttgcagc taatcaagtg tttgcggctg ctgttaacga ggatcagacg
    23221 ctttttcaag tgcttcgaaa tggagcaaga gtagcctttg tgattcttca agaaaagggg
    23281 gaatttctag aaatgacttt agggattctt gaaacagtga cagaaaacga ggttccagca
    23341 gctattgtgg aggctgtcca gacatatctt ctggcagata tgcaagagaa actcccttct
    23401 ttaagcatta tggtcagtca agaatcaagc aagctttttt accaagaaca agggtttgtt
    23461 ttgctagcta agggttgcaa gccaggaaac gaaaatgatt ctgacgtttg tcagctcgta
    23521 aaatattttt ccaagccatg aagaatcaag gggaatggtt tattcataga aacattttat
    23581 catcaaagtg gtataataag accatgtcaa aaaaacctac ttcagcctat gtgcacattc
    23641 ctttttgcac acaaatttgt tattattgcg atttttcaaa agtctttatc caaaatcaac
    23701 ctgttgatgc ttatttgaaa gccttgattc aagaatttga ctcttatggc attcgagacc
    23761 taaaaaccct ttatattgga ggaggtacgc ctacagctat cactgctaaa cagttggagt
    23821 atctcctaaa tcaccttgaa cgaaacctca atttagatga tcttgaagaa ttcacaattg
    23881 aggcaaatcc tggtgatttg acgccagaaa aaattgcggt gcttcagagg tcagctgtca
    23941 atcggatttc cttgggggtg cagaccttta ataataagca attaaagcag atcggccgta
    24001 gccataatga agaacaaatt tatagcacca tcgctaattt aaaagctgca ggctttcaca
    24061 atatttccat tgatttaatt tatgccttgc caggtcaaac cttagatcaa gtcaaggaaa
    24121 atgttgctaa agctttggca cttgatattc cccatcttag tctttatagc cttattttgg
    24181 aacatcacac cgtttttatg aacaaaatgc ggcgtggtaa acttaactta ccaacggaag
    24241 acttggaagc agaaatgttt gagtatatta tttcagaaat ggaagctaat ggctttgaac
    24301 attatgaaat ctccaatttt accaaacctg gttttgaaag tcgtcataac ttgatgtatt
    24361 gggataatgt ggaatatttt ggatgtggtg caggggcttc gggttacctt aatggcattc
    24421 gctatcaaaa tagggtaccg attcagcatt atcttaaagc ggttgaagca ggaaacgcta
    24481 gactaaatga agaagtgcta cgcaaaaagg aaatgatgga agaggaactc tttttgggct
    24541 tacgtaaaaa gacaggagtc tccatccagc ggttccaaga aaaatttggt atttcttttg
    24601 aggagtgtta tggaaatatt gttagggagc ttcaaaacca aggactgctt gtcaaagatg
    24661 acgcctttgt cagaatgact aaaaagggtc ttttcttagg tgattctgtt gctgagcggt
    24721 ttatattaga ttaggagagt attatgggat taagttatca ggaggagttg acacttcctt
    24781 ttgaattatg tgatgtcaaa tcagatataa aattgcccct tttattagac tattgtttga
    24841 tggtttctgg tagacagtct gcgcaattag gacgaagtaa caacaatctt ttagttgatt
    24901 acaagcttgt ttggattgta acggattatg agatcactat tcatcgcttg ccacattttc
    24961 aagaaaccat caccattgaa acaaaagccc tttcctataa taaatttttt tgttatcgcc
    25021 aattttatat ttatgatcaa gaggggtgcc ttttagtgga tatcttagcc tattttgctt
    25081 tgttaaaccc agatacgcga aaagtggcaa ctattccaga agatttagta gcgccatttg
    25141 agactgattt tgttaaaaag ctacaccgtg ttcctaaaat gcctctttta gaacaatcaa
    25201 ttgatcgtta ttattatgtg cgttattttg atattgatat gaatggtcat gtcaacaaca
    25261 gtaaatattt agattggatg tatgatgtgt tggggtgtga gtttttaaaa acgcatcagc
    25321 ctcttaagat gactttgaaa tatgttaaag aagtctcacc aggcggtcaa attacttcca
    25381 gttaccattt ggaccaatta acttcttacc atcaaatcac ctcagatggg cagctgaatg
    25441 cccaagccat gattgaatgg cgagcgatta aacaaacaga aagtgagata gactagtgcc
    25501 ttataagggt tatttaattg atttagacgg aacgatttac caagggaaaa accgtattcc
    25561 agcaggggag cggtttatca aacgcctaca agaaaaaggc attccctatt tattagtgac
    25621 taataatacc acaagaacac ccgaaatggt gcaaagcatg ctgactaatc agtttcatgt
    25681 tgagacaggc attgagacca tttatacggc taccatggca accgtcgatt acatgaatga
    25741 catgaaccgt ggtaagacag cttacgttat tggagaaaca gggctcaaat cagctattgc
    25801 agcagcaggt tacgtagaag aacttgagaa tcccgcttac gtggtggttg gtttagacag
    25861 tcaggtgacc tatgagatgt tggctatagc caccttggct attcaaaaag gtgccctctt
    25921 tattgggaca aatcctgatt taaacattcc aaccgaacgt ggtttgatgc caggagcagg
    25981 tgcattgaat gccttactag aagctgccac acgtgtaaaa cctgttttta ttggcaagcc
    26041 caacgctatc attatgaata aatcactaga ggtattgggc attcagcgct ctgaagccgt
    26101 catggttggc gataattact tgactgatat catggcaggt attcaaaatg atattgcaac
    26161 gatcctcgtg acaacaggct ttaccagacc tgaagaggta ccaaccttac caattcagcc
    26221 agatcatgtc ctatctagcc ttgatgagtg gaggttgtga tggtagaaaa cactaaatta
    26281 ctatgtagct gggtatggtt attagcgtta gctattctca taacgatcta ttcaacgtgg
    26341 ctgtggtatc ctcttgaggt ggaccatcta aagttagaac aagttgtttt tatgagcaag
    26401 gatgctatct tgcataacta taacggacta ctaaattacc tcaccaatcc ttttgtgaca
    26461 aggttggaat ttgctaattt tcactcatca gcagacggac tcaagcattt tgcagatgtg
    26521 aagtggcttt ttcacctaac tcaagtcgtt tttcttggcc tcctttaccc aacccttaag
    26581 actttcacac aaaggttaaa aaccaaacgt ttctggctcc ttcaaaaacc tttaatacta
    26641 gcagctttgt ttcctcttat gattggtcta atggctagtt tcattgggtt tgaacatttt
    26701 tttactcttt ttcatcaggt gctttttgta ggagatagca gctggctctt tgatcccttg
    26761 aaagatcctg tgatttggat ccttccagaa gtctttttct tgcactgttt ccttttcttt
    26821 atgatagttt acgaaatcat cctgtggagc ttagtaggtt tagcaagatg gcagcgccta
    26881 aatgtagaat gataaaacaa gcgttttgaa agcgcttgtt tttttagttt gcagtcagag
    26941 tagaatagaa gtatcaaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
    27001 actagcctta ttttaacttg ctatgctgtt ttgaatggtc tccattcaac attgccgatg
    27061 gtaacttgag aaagagggtt aataccagca gtcggatacc ttcctattct ttctgttaaa
    27121 gcgttttcat gttataatag gcaaaagaag agtagagtga tggaacaaac attttttatg
    27181 attaagccag atggggttaa gcgagggggt tagttggaga ggttttgcgg tggattgagc
    27241 gcctaagact tacgtttaag cgattcgagc taagacaagc tagttcgaga tacttggcta
    27301 agcacgacga ggccttggtg ataaaccttt tgatcctaaa cctaaagctt acatgacaag
    27361 tggtcctgtt ttaattggga cacttcttgg ggactaaggt ggttttgtct ttttgaacca
    27421 tgatgagagt aaccaatcct acggatgctc tttgcggaac cattcgtggg aattttgctc
    27481 aggctcctgg tgatgatgga ggtgttttta acatggtgca tgagtctcat tcaagagact
    27541 ctgctaggcg tgagattgtc ctttggtctc atcaaagtaa tcttggtagc tccgctattc
    27601 tcttgcaaga taatccgtaa gaaaagatag gcaaaggcct accttttttg ttataataga
    27661 aggaaagact aaatgtatag gaaaatagaa tgaacagtca agatttaaaa aaacgtcagg
    27721 agaagattcg taatttctcc attattgcac atattgacca tggcaaatct actctcgctg
    27781 accgcatttt ggaaaagaca gagacggttt cgtctcgtga aatgcaggct cagttattgg
    27841 actccatgga tttggagcgt gagcgtggga ttaccatcaa gttaaacgcc attgagctta
    27901 attacacggc taaagatggg gaaacctaca ttttccacct tattgatacc ccagggcatg
    27961 tggactttac ttatgaagta tcgcgttcgt tggcagcttg tgagggagcg attctagttg
    28021 tggatgcagc gcagggtatt gaagcacaga cccttgccaa tgtttaccta gcccttgata
    28081 atgatttgga aattttaccg gttatcaata aaattgattt gcctgctgct gaccctgaga
    28141 gagtccgcca tgaagtagaa gatgtcattg gacttgatgc ttctgaggct gttctggcat
    28201 cagccaaggc tggtattggg atcgaagaga ttcttgagca aattgttgag aaggttcctg
    28261 ctcctacggg tgatgtggat gcgcctttac aggccttgat ttttgactct gtgtatgatg
    28321 cttaccgagg cgttatcctg caagttcgga ttgtgaacgg tattgtcaag cctggcgata
    28381 aaatccagat gatgtcgaat ggaaaaactt ttgatgttac agaagttggc attttcactc
    28441 ctaaggcagt tggacgagat ttccttgcaa cgggagatgt tggttatgtt gcggcttcta
    28501 tcaaaacggt agcagatacc cgtgtggggg atacggtgac tttagctaat aaccctgcta
    28561 aggaagcctt gcatggctac aagcagatga acccgatggt ctttgcaggg atttatccga
    28621 ttgaatcaaa taaatacaat gacttgcgtg aagcgcttga aaaattacaa ctgaacgatg
    28681 ccagtttaca gtttgaaccc gaaacgtcac aagcgcttgg ctttggtttt cgatgcggct
    28741 ttttaggctt gcttcacatg gatgtgattc aagaacgttt ggaacgtgaa tttaacattg
    28801 atttgatcat gacagcaccg tccgtggtgt atcacgttca cacaacagat gaggacatga
    28861 ttgaagtgtc taacccttca gaatttccag acccaacccg ggtggctttt attgaagagc
    28921 cttatgttaa agcgcaaatc atggtgccac aagagttcgt aggggctgtt atggaattat
    28981 cgcagcgcaa gcgtggtgat tttgtgacca tggattacat tgatgataac cgtgtcaatg
    29041 tgatttacca aatcccgctg gctgaaattg tctttgattt ctttgataaa ttgaaatcct
    29101 caacgcgagg ctatgcgagt tttgattatg acatgtcaga ataccgcagg tcacaattgg
    29161 tgaaaatgga cattcttttg aacggtgata aggtcgatgc gctcagcttt attgttcata
    29221 aagaatttgc ttatgaacgc gggaaaatta ttgttgagaa attgaaaaaa atcattccac
    29281 gtcaacagtt tgaagtgccg atccaagccg ctattggtca aaaaattgtt gcgcgttcag
    29341 acatcaaggc ccttcgtaaa aacgttttag cgaagtgtta tggtggtgac gtgtctcgta
    29401 aacgtaaatt gctcgaaaaa caaaaggctg gtaaaaaacg gatgaaggct attggttctg
    29461 tcgaagtccc tcaagaagcc ttcttgagtg tgctttcaat ggatgacgat accaagaaat
    29521 aaccaagctt aatcgcttag tcttcagagg agaactcttg tgggtctaca tgaccaaaaa
    29581 attgattatc ccataaaaaa agaactttac aatctctttc acataacact ataatagtac
    29641 tatagtgtta tctcattatt aggtgagcct acatttaatc ttattaaaga gaaagaggaa
    29701 acaaaaactg t
//
LOCUS       DAUCWU010000013        27703 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUCWU010000013 DAUCWU010000000
VERSION     DAUCWU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.013"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            32..1306
                     /locus_tag="VGY24_000565"
     CDS             32..1306
                     /locus_tag="VGY24_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ4283573.1"
                     /translation="MKKLNVILVGLLSILMLSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSDLVIKAVKHIKKDQKDYYYFSPIKQADDFFVDNLPVSLYKKKNSDKELILVKPKLQ
                     SSHLRSVNTLTISKIVYQKKFFHLAKKSEKVISTYHVTDDLKPFQVKDLVSGHLERIQ
                     EEVEKKYPDAGFNSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPD
                     FLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDSTTTPQVLDILAKYQAKGTFFMIG
                     SKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPR
                     YLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDI
                     HQTTINALPTVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1417..2502)
                     /locus_tag="VGY24_000566"
     CDS             complement(1417..2502)
                     /locus_tag="VGY24_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4283574.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2600..3226
                     /gene="udk"
                     /locus_tag="VGY24_000567"
     CDS             2600..3226
                     /gene="udk"
                     /locus_tag="VGY24_000567"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ4283575.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            3306..3569
                     /locus_tag="VGY24_000568"
     CDS             3306..3569
                     /locus_tag="VGY24_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ4283576.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERKLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3622..4431)
                     /locus_tag="VGY24_000569"
     CDS             complement(3622..4431)
                     /locus_tag="VGY24_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ4283577.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISVFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVIALLSGMGFQL
                     TEDIRYLIQAKSIDNLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4576..5073
                     /locus_tag="VGY24_000570"
     CDS             4576..5073
                     /locus_tag="VGY24_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ4283578.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKESHLIGVLDLDSSLVADYDEVDQEYLEAFVDLLLEKTTFTFNM
                     FGVKN"
     gene            5073..6743
                     /gene="dnaX"
                     /locus_tag="VGY24_000571"
     CDS             5073..6743
                     /gene="dnaX"
                     /locus_tag="VGY24_000571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ4283579.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISKTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            6838..7041
                     /locus_tag="VGY24_000572"
     CDS             6838..7041
                     /locus_tag="VGY24_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ4283580.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
     gene            complement(7016..7957)
                     /locus_tag="VGY24_000573"
     CDS             complement(7016..7957)
                     /locus_tag="VGY24_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEQ4283581.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFDNGDLQTLRSGEISLSSWEN"
     gene            8161..10539
                     /locus_tag="VGY24_000574"
     CDS             8161..10539
                     /locus_tag="VGY24_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ4283582.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSLVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHQTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNMM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKQLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLRSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAH"
     gene            11076..12272
                     /locus_tag="VGY24_000575"
     CDS             11076..12272
                     /locus_tag="VGY24_000575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ4283583.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            12446..13705
                     /locus_tag="VGY24_000576"
     CDS             12446..13705
                     /locus_tag="VGY24_000576"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ4283584.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLMAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            14098..15642
                     /locus_tag="VGY24_000577"
     CDS             14098..15642
                     /locus_tag="VGY24_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4283585.1"
                     /translation="MEKTIDQRVEEVTYNRAKVWQLVLFALNNTSTNIYLFAFMFITY
                     FSTGVLGLAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIAGNIITAL
                     SLILLLALNNVSEGMRFPLFILVLIVHKIGYSMQQTITKAGQTALTNDPKQRPIFNIV
                     DAVMLTTLMTGGQFVVSGFLVPKFGNFTLEFFNVLIYGTIVISAILGLLAVIGIWSKD
                     NKEYFGLGENTQKTELKDYMKVIKGNKPLQVLSIAAALVKFSVQFFGDSVVMVMLYGI
                     VFGNYALSGQFSLLFVIPGVLINILISNIARKKGLRFSYVASLQFGLIALAAFGVVLY
                     FGKVGDLSLSNINLYTICFIITYMLARYITQAPAGLVLTMGADISDYETSESGRYVSG
                     MIGTIFSLTDSIASSFAPMVVGAVLATIGFAKAFPTMETPLTPELKMATIILLVGIPF
                     VALSVALFLMRFYKLDKEEMVRIQEKIQVMKAASSKERVKDIARNVPLSDMDYVDVTK
                     YPVDKD"
     gene            15797..16825
                     /locus_tag="VGY24_000578"
     CDS             15797..16825
                     /locus_tag="VGY24_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ4283586.1"
                     /translation="MKTVVSLGEILLRLSPPQYQTLTQATSLECQFGGSELNVLSTLA
                     QLGHKVSLISAIPANDLGRMTKTFLFSRQISQDYIIEKGQRLGIYYYQKGFSLRASRV
                     TYDRKYSAFWESELADYELADVFAGADWFHVSGITPALTDKLYHLTHYLMTKAKTEGL
                     KVSFDLNFRASLWDSFQEARELLSPLVALADVCIGIEPLVLAGDGEKDLKDQLGLSRP
                     YEDQALLENVLSKLAKTYGVTSFAFTQREMGHTNEYLLKGYLYQDGNLYQTDKSGIQV
                     LDRVGTGDAFTAGLIHGMLQKEVPDKALAIAMASFKFKHTIEGDSNIITPADIDYLIT
                     DASHEIKR"
     gene            16849..18639
                     /gene="uidA"
                     /locus_tag="VGY24_000579"
     CDS             16849..18639
                     /gene="uidA"
                     /locus_tag="VGY24_000579"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEQ4283587.1"
                     /translation="MLYPTLTKTRSLYDLSGLWQFKLGDHNPEDCLSTEDVMVVPTSF
                     NDVVVDKDKRNYIGDFWYERLVDLPKLAKDDELVLRFGSVTHNATVYVNGRELASHKG
                     GFTPFEVVVPEELYQDHQIRISVRANNILDYSTLPVGNYSEERQEDGSMKKVVKENFD
                     FFNYAGIHRPVKLVVRPKTHISDITITSDLSDDLKTADLSIAVETSAAVDAIQVTILD
                     EERQVVGQTNDGHVKLDCVHLWEVLDAYLYTARIEISVDGQIVDTYEEPFGVRSVHVA
                     DGQFFVNRKLVYFKGFGKHEDTFINGRGLNEAANLMDMNLLKEIGANSFRTSHYPYSE
                     EMMRLADRMGILVIDEIPAVGLFQNFTASLDLTPKDMGTWSVMETKEAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVTLYKELDPQKRPVTLVNILMATPDRD
                     EVMDLVDVICLNRYYAWYVDHGDLAKGEAGLRKELLEWQSKFPDKPILMTEYGADTLP
                     GLHSMWDIPYTEEFQCDFYAMTHGVFDEIPNLVGEQVWNFADFETNLMIFRVQGNHKG
                     LFSRNRQPKQVVREFKKRWTAIPNYNYKKK"
     gene            18681..19352
                     /locus_tag="VGY24_000580"
     CDS             18681..19352
                     /locus_tag="VGY24_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEQ4283588.1"
                     /translation="MARPLVEKTAERLLQLILERGYDVGAKLPNEYELAQDLDVGRST
                     IREAVRSLATRNVLEVRQGSGTYVSSKKGVSEDPLGFSLVKDTAKLTADLFELRLLLE
                     PRIAALAAQNATPKEIKELEKIVVEIEEAVTVGDPKHLQLDVTFHSMMAKFSGNIAMD
                     SLLPVINKSIHLINANYTNRQMKEDSLQAHRDILTAIKERNPIAAHDAMLLHIMTVKR
                     TAFLP"
     gene            19480..20097
                     /locus_tag="VGY24_000581"
     CDS             19480..20097
                     /locus_tag="VGY24_000581"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEQ4283589.1"
                     /translation="MLTQLKENYFFAVVRGKTAEDAIEIAKYAVLGGIRNIEITYSTP
                     NASEVISKLSDDFKDDTSVVIGAGTVMTTELAKEAIAAGAKFLVSPHFSKEIADIANQ
                     EGNLYFPGCATATEIVTAMNASCPIIKVFPGGVVGPSFIKDIHGPIPEVDLMPSGGVS
                     VDNVKEWKNAGAVAVGVGSALASKVATEGYYSVTRIAQSFVSALD"
     gene            20127..21525
                     /gene="uxaC"
                     /locus_tag="VGY24_000582"
                     /pseudo
     CDS             20127..21525
                     /gene="uxaC"
                     /locus_tag="VGY24_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            21620..22714
                     /locus_tag="VGY24_000583"
                     /pseudo
     CDS             21620..22714
                     /locus_tag="VGY24_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
     gene            22729..23568
                     /locus_tag="VGY24_000584"
     CDS             22729..23568
                     /locus_tag="VGY24_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ4283590.1"
                     /translation="MTRVIDFKDKVVIVTGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQAFVTEIEAEGGVAKAYKANVLSKENLEEVRQAVLADFGPADILVNGAGGNSPKAT
                     TGNEFHEVGLPEETKTFFDLDKAGISFVFNLNYLGTLLPTQVFAQDMVGRKGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRGLLF
                     TEEGQPTARAEKILNNTPMGRFGEAEELIGGLFFLADEQSASFVNGVVLPIDGGFAAY
                     SGV"
     gene            23837..24640
                     /locus_tag="VGY24_000585"
     CDS             23837..24640
                     /locus_tag="VGY24_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ4283591.1"
                     /translation="MTTKKEAFVFCVDSDGCAMDTMTYKHQLFFGPIAADVFGVTNRE
                     AFLKEWNRVNLYSRTRGVNRFVGLVMGLDYAGLKGIDRLKDWVANTKSLSNASLEAEL
                     AKEASDDLQKALEWSNEVNRQIKAYEGEALAFPGAESGLEKLHQLGKVFVVSSANKEA
                     VEEEWHDQGLMAHVDDLYCQDCGKKEDVIAELLQKGYQKERLMMVGDSPGDLAAAEQN
                     QVAFFPILVGKEAQSWADLKEVVADAFVTGELSDLDFNSLKETFWHNLD"
     gene            24757..26547
                     /locus_tag="VGY24_000586"
     CDS             24757..26547
                     /locus_tag="VGY24_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEQ4283592.1"
                     /translation="MTHLVDLRKKPYHLDDQAIAWVEETIASMSLEEKIGQLFVNMGA
                     SRTEEYLTQVLNDYKIAAVRYNKGMADEVWEQNYILQTKSKIPLLIAANTEAGGDGAV
                     NDGTKVGDEIKVAATNDPKYAYEMGRIAGIEAAAVGCNASFAPIVDLTRNWCNPIIAS
                     RNWGSNVDQIITFSKEYMRGIMEHGIMPFAKHFPGDGIDERDHHLSFASNPMTKDEWM
                     STFGRIYGELTEAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVAMTASMPRRLMLPTAVEAGCDLFLFFNDPDEDLQWMKEGYEAG
                     ILTEERLHDALRRTLGLKARLGLHLYEGRREEIMLPKEEAMTLINTKEAQAIADEVAD
                     KAITLVKDKQKDIFPVTPERYKRILIVNVEGHKGGFGAMIAGQKKRASDVVKELLEAR
                     GHDVTVWESTEERIMQLPESERAAAIANIYAQKQPISNLTDHYDLILNLVDVNSGGTV
                     QRIVWPAAKGTPDQPFYVHEIPTIVVSVQHAFALADMPQVGTYINAYDGKDNTMKALV
                     EKLAGESNFTGVSPVDAFCGLIDTHLWREY"
     gene            26593..27644
                     /locus_tag="VGY24_000587"
                     /pseudo
     CDS             26593..27644
                     /locus_tag="VGY24_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8569 a         4898 c         6053 g         8183 t
ORIGIN      
        1 ttacaatagt ctatacagat ggaggcaact attgaaaaaa ttaaatgtta ttcttgttgg
       61 tttattaagc attctgatgt tgagtttagc tattgtgttt attaatcgtt ggaaactaaa
      121 cgaagatagt cagcgtatag ttttggctga aaagaaaaaa aacacgtcag atttagtgat
      181 caaagctgta aaacatatta aaaaagatca aaaagactat tattattttt ccccgataaa
      241 acaagcagat gatttttttg tagataattt acctgtttca ttatacaaaa aaaagaattc
      301 agataaagaa ttgattttag taaagcctaa actgcaatct tctcacctaa gatcagttaa
      361 cactttgact atttctaaaa tagtttatca gaaaaaattt tttcatttgg ctaaaaaatc
      421 agaaaaagtt ataagtacat atcacgttac agacgacttg aaaccttttc aggtaaagga
      481 tctagtatca ggacatttag aaagaataca agaagaagtt gaaaaaaaat atccagatgc
      541 tggttttaat agcgataagt ataatggctt aaaagaatct aattctttat taagcgatgg
      601 ctttgaggta aaatcgggaa accttatttt tgataaaaag ctaacgatac ctttgacgac
      661 attatttgat gttattaatc cagatttttt agcaaatagc gatagagctg cgtatgataa
      721 ttataggacc tacaaagaac agcatcccaa aaaactagtt gcattaacgt ttgatgatgg
      781 tccagattcg acgacgactc ctcaagtttt agatattttg gcaaaatacc aggctaaggg
      841 aactttcttt atgataggtt caaaagttgt gaataatgaa aaccttacta aacgtgttag
      901 cgacgctggc catgaaattg ctaatcatac ttgggatcat cctaatctga ctaatctttc
      961 agtcagcgag attcaacatc aagttaatat gacgaaccag gctattgaaa aagcttgtgg
     1021 caagaaacct cgctatttac gccctccata tggagctaca aatgcaactg ttcagcaatc
     1081 ctcaggatta acacaaatgc tttggacggt tgatacaaga gattgggaaa atcatagcac
     1141 tgatggcatt atgactaatg tcaaaaacca attgcaaccg ggaggagttg ttttaatgca
     1201 tgatattcat caaacaacta tcaatgcttt gccgacagtt atggaatatc taaaagcaga
     1261 aggatacgaa tgtgtgactg tatcagaact ctatgcgcat cagtaagtaa tatcgttatg
     1321 tttctttaga attgatatac taaacaatcc aattactttt gaagtaattg gattgttgtt
     1381 tttagtagcc cttgattaag ttttgttttt taacaactag ttgatgaaat gaagttgttg
     1441 atttttgagg gagatttcag agactttagc aaattttttc aattttttga ggtcttcagg
     1501 gtggctaaca aaggtaataa cgataccaga ttttcccata cggccggttc gtccagctct
     1561 gtgagtgtaa ttttccttat cacgagcaac atcaaaatga atgacatagt caagattatc
     1621 aatatcaatg ccccgagcaa caagatccgt agctaacaat aaggagagtt ggtgggattt
     1681 gaatttttcg agaatggttt tacggaattt gacattgatg tcactcgcta aggaaaccgc
     1741 agcagctcca ttatattgga gacgttcttc cgtagcaccc aaatcagata aactgttgaa
     1801 aaagaccaaa gctctaaaat gaggaatatt agcaaatttt cgtaataaat cggtacgttc
     1861 gcgcttgtca accatgaggt aaaaatgctg aatagcatcg ttggtttgct ctgaaagatc
     1921 gatgcagaat gtatttggag ctaaggaagt ttgatccacc ttattagtag cactcatgta
     1981 taccatttga tgatctcgag gaacatagtg gctaattttt tgaacaaaat catattggga
     2041 atcacctaat aattcgtcat attcatctaa aacaatggtg ttgacagaca tcatcttgat
     2101 ttttttcaat ttaatgagtt caaaaatgcg tccaggggtt ccaatcagga tttcaggacc
     2161 ttttttaagg cgttcgattt gacgtttttg actggttcca gaaataaaga gttgtgccgt
     2221 taagcctagt ggctgtgccc aatcctttgt gacttcaaaa atttggccag ctaattcggt
     2281 attaggggct aaaatgagta gttgttggga ctttttaggc gtcagtgcca atagactagg
     2341 aaagacataa gctaaggtct tgcccgtccc agtagggcta ataccaagga aatttttacc
     2401 atcaacaata ggttgaaagg cctgttcttg gattggcgtt aaatgggtaa atgcaacttg
     2461 gtcaagtttt tcttgccatt gagggggaaa tttagtgatc atgcgtcttc cttttactat
     2521 tgtttctata ttcattatag cattatagtc tcaattttgg taaaatagtg agaaagactt
     2581 tttagatttg gagtttaaaa tgcttaaaaa accgattatt attggtgtaa ctggtggttc
     2641 aggtggcggt aaaacaagtg tgtcacgcgc tattttggac agttttccaa atgctcgtat
     2701 tgcgatgatt caacacgatt cttactataa agatcagtct cacatgagtt ttgaagaaag
     2761 ggtcaaaacc aattatgacc atcctctagc ttttgataca gattttatga ttcagcaact
     2821 aaaagaatta ttagctggta gacctgtaga tattcctatc tatgattaca aaaagcatac
     2881 ccgcagtaat acgacctttc gtcaggatcc tcaggatgtt attattgttg aaggtattct
     2941 tgtcctagaa gatgagcgtt tgcgagattt gatggatatc aagctttttg tagacacaga
     3001 tgacgatatt cgtattattc gtcgtattaa gcgtgacatg atggaacgtg gcagaagctt
     3061 agaaagtatt attgatcagt acacaagcgt ggtaaagcct atgtatcatc agtttattga
     3121 gccaagtaaa cgctatgctg atattgttat tccagaggga gttagtaatg tcgttgctat
     3181 tgatgttatt aatagtaaaa ttgcgagtat tcttggagaa gtatagtgga agtggctgag
     3241 tagattattt aaatctgctc ttttattgtt gtgctcttcc tagagaaagg gatcataaaa
     3301 tagaaatgtt attaatttgg cttggattac tatttgtttg gaatgaactc gtgtttgtca
     3361 tatatgcctt tgataaaaga aaagccataa aaaagaaacg gcgtattagt gaaagaaaat
     3421 tattagtgat aacagttttg tttggaggtt ttggagcatt acttgcagct aaaaagtacc
     3481 accataaaac cagaaagtgg tactttgtca tcacttggta tactagtatt ttgttgacat
     3541 tactagtaac ttatatcttt atgaagtgag tcacttaaaa gacccttaat cagtattatt
     3601 tagtcaaggc tcttttatag tttaaaaaag cacatcatcg tcaagggtgt tgattttttg
     3661 aaaaagatca ccaaagatta aaaggattaa tgttcctagt aaaactgtca tgccaggaat
     3721 gcttgttaga ggagagttcc agatgaaatg tagaaccaga ggacttaata accagaaaat
     3781 ttgttgctgt tttgaaaaaa ggttagaacc ctttaataat aagtatgctc caatggtaaa
     3841 aatagctgta tgtacccaat gtgaagtcac agcagtgcta atacgttcaa tagcagtcgg
     3901 tacgagatta tctatagatt ttgcttgaat caaataacgg atatcttctg ttagttgaaa
     3961 gcccatgcca gaaagtaaag caataactaa tttttcttta agagtaagtc taggaaataa
     4021 agtaattact aaaatagccg aagaagcctt aatgggctct tcgacaatag gggcagttaa
     4081 agcatctccc caaagcttta aagcgtcttt agaagggatg atgtatgacc aaaattgtcc
     4141 tacgagatgg ttcccacaag aagctaaaaa aactgaaata taaagtccgc taagacagct
     4201 aacaataata acgttcaggt tcatatgaaa ttttttactt aaataaaaaa tagctgctgc
     4261 aaatggaatt aagtaaatgc ctaatagact aatggttagt attagagcaa aagcttcttt
     4321 aaaggaaagg ttgttagctg tcagtcctaa caattcaagt tcaataccat ttaaaactaa
     4381 agcaataaaa agaccgtatc gtaaccattt ctgttgagtg atttgtttca cgaaaacctc
     4441 ctaatattaa gatgatatta ataatattga aaattatatc ataaacaacc tcaaaaacca
     4501 ttttaaaaac tgactgaagc gaaagatagt cgtaaaaaat actcgaatag acccagatat
     4561 gctataataa aaggtatgaa taagtctaaa aaaattgagc aataccaact aatgatagcg
     4621 caggcaaaag agctgtttgc gaatgaatca aatgctttgg ctaatttgtc aaatgccagc
     4681 gccctgttaa acatgacact acccaattct gtttttgcgg gattttacct ttttgatggg
     4741 caggaattaa ttcttggccc ttttcaaggt agggtttctt gcgtccatat caagcttggt
     4801 aaaggtgttt gtggcgaatc agctcagtct cgtcgaacca ttattataaa tgatgtgaag
     4861 cagcatgcta actatatttc ctgtgatgcg gcagccatga gtgaaattgt tgtgcctatg
     4921 gtgaaagaaa gccatttgat tggggtgctg gacttggatt ctagccttgt agctgattat
     4981 gatgaggttg atcaagaata tttagaggcc tttgtggacc tacttcttga aaagaccacg
     5041 tttaccttta acatgtttgg agttaaaaac tgatgtatca agctctttat cggaaatacc
     5101 ggagccaaac gtttgacgaa atggtgggac aatcggttat ttccaaaact ttaaagcagg
     5161 cagttgaatc tggcaagatt agccatgctt atttattttc aggtcctaga gggactggga
     5221 aaacaagtgc ggcaaagatt tttgcaaagg ccatgaattg tcctaaccaa gtcgatggtg
     5281 aaccttgtaa tcaatgcgat atttgccgag atatcacgaa tggaagcttg gaagatgtga
     5341 ttgaaattga tgctgcctcg aataatgggg ttgatgaaat tcgtgacatc cgagacaaat
     5401 caacctatgc gccaagtcgt gcgacttaca aggtttatat tattgatgag gttcacatgt
     5461 tatcaacagg ggcttttaat gcgcttttga aaactttgga agaaccgaca gaaaatgttg
     5521 tctttatctt ggcaacaacg gaattgcata aaattccagc cactatttta tctcgtgtgc
     5581 aacgctttga attcaaagct attaagcaaa aagctattcg agagcattta gcctgggttt
     5641 tggacaaaga aggtattgcc tatgaggtgg atgctttaaa tctcattgca aggcgagcag
     5701 aaggaggcat gcgtgatgct ttatctattt tagatcaggc tttgagcttg tcaccagata
     5761 atcaggtcac cattgcaatt gccgaagaaa ttacaggttc tatttccata cttgctctgg
     5821 gtgactatgt tcgatatgtc tcccaagaac aggctacgca agctctggca gccttagaga
     5881 ccatttatga tagtgggaag agcatgagcc gctttgcgac agatttattg acctatctgc
     5941 gtgatttatt ggtggttaaa gctggcggcg acaatcaacg tcagtcagct gtttttgata
     6001 ccaatttgtc tctctcgata gatcgtatat tccaaatgat aacagttgtt actagtcatc
     6061 tccctgaaat caaaaaggga acccatcctc ggatttatgc cgaaatgatg actatccaat
     6121 tagctcagaa agagcagatt ttgtcccaag caaacttgtc aggagagtta atctcagaga
     6181 ttgaaacgct caaaaatgag ttggcacaac ttaaacaaca attgtcgcag ctccaatcgc
     6241 gtcctgattc actggcaaga tctgataaaa cgaaacctaa aaccacaagc tacagggttg
     6301 atcgggttac cattttgaaa atcatggaag aaacggttcg aaatagccaa caatctcgac
     6361 aatatctaga tgctctaaaa aatgcttgga atgaaattct agataacatt tctgcccaag
     6421 acagagcctt attgatgggc tctgagcctg tcttagcaaa tagtgagaat gcgattttgg
     6481 ctttcgaggc tgcctttaat gcagaacaag tcatgagccg aaataatctt aatgatatgt
     6541 ttggtaacat tatgagtaaa gctgctggtt tttctcccaa tattctggca gtaccaagga
     6601 cagattttca gcatattcgt aaggaatttg ctcagcaaat gaaatcgcaa aaagacagtg
     6661 ttcaagaaga acaagaagta gcgcttgata ttccagaagg gtttgatttt ttgctcgata
     6721 aaataaatac tattgacgac taaaactgaa atacataagg gtaaggtttt gcccttaccc
     6781 acgttttatc tcgttacttt tcttcatagt acagtatttt tttgttataa taaagctatg
     6841 actattcgac aatttctttt tatggccttt gtatgtgcct ttgaaaccta tttttttaat
     6901 gacctgttgc tctctggaaa ttatctcttc gccttatttt ggggcttgtt gctctttagg
     6961 gatttaagaa gagtgcatac cattaaccaa ttgactaaaa ccattttaaa aacggctaat
     7021 tctcccaaga agaaagactg atttcaccgc ttcgcaaggt ttgaaggtca ccattgtcaa
     7081 attggacaat aaggtgtcct tgatctgtga gatcaatcgc aatagcacgt ttttcttgac
     7141 tattttctat aaaggtcact tgtttattaa gaacaagtga tttttctttg taaactttga
     7201 catggtcctt gacaggaata gataagaata atttccagat gtcaatgata aggtcattgc
     7261 gcgtgatagt cggtttttcg gtaaagagag aacctgcctt ttgagcaatc gcttctggga
     7321 agtcggtgac aaaaaagttg agaccaaccc caataatgac atcggtgatt aaaccagttt
     7381 caacagaagt aatggcttct gtcagaatcc ctgcaacttt atggttacct agatagatgt
     7441 cgtttaccca tttgatttct gtatcaatgc ctgttaatcg cgagatggcc ttgacaatac
     7501 tggaagcaac catcatcgta taaggaggca tatcagcata agggacattg ggttttaggt
     7561 acatggacat gtaaatacca ccggtgctag ctgaaaaaaa ttgccgatcg aggcgtcctt
     7621 tggcagcttc ttggctagga gctaggtaaa gccttggagc gctgttatga gcttcgattc
     7681 cttgtttggc atctaattgg gtagattggc tgtgaggggt ataggtgact ggcatcccaa
     7741 gcccttggct aatggttttg ggtaataaaa tatcaccttg taccatgcgg tagcccttgt
     7801 gttttaaaga atcaatttga atcccctgat tttcgagact tttgatggat ttccaaacgg
     7861 atgtacgaga gatactgagt tgatctgcta gatattcccc actgacgaag tcatcggttt
     7921 gtgacagtaa ttgataaatt ttttctgatg ttttcatatg tatagtatat tataaaagat
     7981 cttattttca aaggaaaaga aaagttagtt tttttgtgta atgctactaa caacagaaac
     8041 tattgtttgt tgaagcttca gagctccaac tcgtcaacaa ctaatagtta tcctaaaaag
     8101 agcttgagga aaaacaagtg atgcgatata cttaaccagt taaataaaaa ggagggttga
     8161 atgaaaacga aaaaagttat tattttagtt ggtctattgt tatcatctca gttgactttg
     8221 atagcttgtc aatcacgagg taatggtaca tatcccatta aaacgaaaca atcacgtaag
     8281 ggaatgacgt caaacaaaat taaaccgatt aaaaaaagca aaaagacaaa caagactcac
     8341 aaaggtgtgg cgggtgtcga ttttcctaca gatgatgggt ttattttaac caaagactca
     8401 aaaatcttat caaaaacaga tcagggaatc gttgttgacc atgatggtca ttcgcatttt
     8461 attttttatg ccgatttaaa gggaagtcca tttgaatacc ttattccaaa aggagcaagt
     8521 ttagctaagc cagctgttgc tcagcaagca gctagtcaag ggacttctaa agtagcagat
     8581 cctcatcacc attatgaatt taacccagcg gatattgtgg ctgaagatgc tttaggctac
     8641 acggttcgcc acgatgatca cttccattat attttgaagt caagcttatc aggtcagaca
     8701 caggcacaag ctaaacaggt tgctactcgc ttgccacaaa ccagtagcct tgtttcaaca
     8761 gctacagcta atggtattcc aggcttgcat ttcccaacct cagatggttt tcaatttaac
     8821 ggtcaaggta ttgttggggt aacaaaagac agtattttag tggaccacga tggtcactta
     8881 catcctattt cttttgcgga ccttcgtcag ggtggctggg cacatgtggc agatcaatac
     8941 gatcccgcta aaaaagcaga aaagccagca gaaacccatc agacaccaga gctatctgaa
     9001 cgtgaaaagg aataccaaga aaaattagct tatttggcag aaaaattggg gattgatcca
     9061 tcaactatta aacgtgtgga aacacaagac ggtaaacttg gtttggaata ccctcaccat
     9121 gaccacgcac acgtattgat gttatctgat attgaaatcg gaaaagacat tccagatcca
     9181 catgctattg agcatgcccg tgaattggaa aaacataagg ttggaatgga taccttgcgt
     9241 gccttagggt ttgatgaaga agtgattttg gatatcgttc gcactcacga tgctccaacc
     9301 ccattcccat caaatgaaaa agatccgaat atgatgaaag aatggttagc aacggttatc
     9361 aaacttgact tgggcagccg taaagatcct ttgcaacgta aaggactttc actgttaccc
     9421 aacttagaaa ctttaggaat tggctttaca ccaatcaaag atatctcacc tgttttgcaa
     9481 tttaaaaaat tgaaacagtt gttaatgaca aaaacagggg tgactgatta tagatttttg
     9541 gataatatgc cacagttaga aggcattgat atttcacaaa acaatctcaa agatattagt
     9601 ttcttgagca aatataaaaa cttaactcta gtagcggctg ctgataatgg tattgaagat
     9661 attaggccgc ttggtcaatt accaaatctc aaattcctcg tattgagtaa taataagatt
     9721 tctgatttaa gcccactggc atcgttacat caattgcaag aattgcacat tgataataat
     9781 cagattacag atttaagccc tgtttctcat aaagaatcat tgacggttgt tgatttatca
     9841 agaaatgctg atgttgactt agcaacactt caagcaccca aattagaaac gttaatggtc
     9901 aatgatacca aggtttctca tttggatttc ttgaaaaata atcctaatct atctagccta
     9961 tctattaacc gtgcgcaatt gcgatctctt gaaggtattg aagcaagtag cgtcattgtc
    10021 agagtagaag cagaaggcaa ccaaattaaa tcgcttgtgc ttaaagacaa gcaagggtca
    10081 cttactttct tggatgtgac aggcaaccag ttgacttctc tagaaggtgt taataatttt
    10141 acagcacttg acattttaag cgtgtctaaa aaccaattaa caaatgtcaa cctatctaaa
    10201 cccaataaga cagttactaa cattgatatt agtcataaca atatcccatt ggcagacctt
    10261 aaattgaacg agcaacatat tccagaagcc attgcgaaaa acttcccagc ggtttacgaa
    10321 ggttctatgg taggtaatgg aacagctgaa gaaaaagcag ctatggctac taaggcgaaa
    10381 gaaagtgctc aagaagcatc ggaatcacat gactacaacc ataatcatgc ctatgaagat
    10441 gaagaaggtc atgctcacga gcacagagac aaagatgatc acgaccatga acatgaggat
    10501 gaaaatgaag ctaaagatga gcaaaaccat gctcactaaa ctaatctaag aataacccta
    10561 tccaactgaa tcgtcctaat gacaccattg ttgactatca agaaaacctc aagaataacc
    10621 ttgaggtttt tttgaaataa ataggataat gagtgaaaag agtggcacct aaatggctaa
    10681 aacatgttat aatagattgt tattgagtcc cgataagacg gtctgttgaa cgaaatgttt
    10741 ttgagacgtt gttgacttca tttggggaac agtcgttctc cctttagcta aattacttcg
    10801 tctaaggctt attttgtggt gacatcctct caacaaggag accggattct ttgtaactca
    10861 acgctattgt cagacattct ttgtctggtt aatggacgct agccactagg tggactagct
    10921 agttgcctta ctagtaaaac cgtaactgat tagctaagtt ggttttggcc acttgacttg
    10981 ctcaatcagc taggaagaaa ggggtaggca acttcttgct ttgtggtcaa tgtgactgaa
    11041 aaaggaagaa tcaacatatt aggggggaca tttttatgtc agaacgtaaa cttttcacgt
    11101 ctgagtccgt atctgaaggg catccagata agattgcaga ccaaatttca gatgctattt
    11161 tagatgccat tttagcagaa gatccagaag cccatgtggc tgcagaaact tgtgtttaca
    11221 caggttctgt tcatgttttt ggagaaatct caacaacagc atacattgat attaaccgtg
    11281 tggtccgcga caccattgca gaaattggtt atacagaagc agaatatggc ttttcagcag
    11341 aatcagtggg agtacatcca tcacttgtag aacaatctgg agatatcgct cagggggtta
    11401 atgaagctct cgaaagcaga gaaggtgata ctgatgacct tagccatatt ggagcgggtg
    11461 accaagggct catgtttggt tttgccatca atgaaacacc agaattgatg cctttaccaa
    11521 tttccttatc tcaccaatta gtgcgtcgct tggcggaatt acgtaagtca ggtgagatta
    11581 gttatctacg tccagatgct aaatctcaag tgacagttga gtacgatgaa cacgataaac
    11641 ctgttcgtgt ggatacagtc gttatttcta ctcagcatga tccagaagct actaatgacc
    11701 aaattcgtca ggatgtgatt gaaaaagtga tcaaggctgt aattccagca gattatttag
    11761 atgatgacac aaagttcttc attaatccaa caggacgttt tgttattgga ggtccgcaag
    11821 gagactctgg attgaccgga cgtaagatta ttgtggatac ctatggaggc tattcacgtc
    11881 atggtggcgg tgctttttca ggaaaagatg cgacaaaagt ggaccgttcg gcttcttacg
    11941 ctgctcgtta tattgctaaa aaccttgtgg cagcaggtct tgcgacgaaa gctgaagtcc
    12001 aactagctta tgcgattggg gtggctcagc ctgtgtctgt tcgcgtggat acatttggaa
    12061 cctcaacggt tcctgaagct gttttagaag ctgctgttcg tcaagtcttt gatttacggc
    12121 cagcaggcat tattcaaatg cttgatttaa aacgcccgat ttacaaacaa acagctgcct
    12181 atggtcacat ggggagaaca gatattgatt tgccatggga gcgtttaaat aaggttgatg
    12241 ctttagtaga agctgtaaaa acagtattat aaacgacaac tttattaaaa aagtagagtg
    12301 agtgaaaaaa tcttactctg cttttttgtt attaaataag cctttaaaag ggatgattaa
    12361 aaattaagac gtcaataatt gtcaggcttc atgatttatg ataaaatgaa aaggttgaat
    12421 tctagtataa gaaagttttg aatgtatgcg taaaattatt attaatggcg gaaaagcctt
    12481 atctggagaa gtggcagttt caggggcaaa aaacagtgtt gttgcgctta ttccagcaat
    12541 catcttagcc gatgatattg tgattcttga tggtgtacct gctattagtg atgtagatag
    12601 tctgattgaa atcatggagc ttatgggagc aacagtaaac tatcatggtg atactttaga
    12661 aattgaccct agaggggtgc aagacatccc gatgccttac ggaaagatta atagtcttcg
    12721 agcttcctat tatttctacg gtagtctttt aggtcgtttt ggacaagcag ttgttggctt
    12781 acctggtggt tgcgatttag gaccaagacc aattgacttg catttgaagg ccttcgaggc
    12841 tatgggagtt gaagtgtctt atgagggtga aaatatgaat ttatcgacaa atggtcaaaa
    12901 aattcatggc gctcatatct atatggacac cgttagtgtc ggtgctacaa ttaatactat
    12961 ggttgcggca acaaaagctc aaggtaaaac ggttattgaa aacgctgcac gtgagccaga
    13021 gattattgat gtagctactc ttttgaataa catgggtgcg catattcgtg gagcaggtac
    13081 agatattatt acgatacaag gtgttcagaa attgcatggt actagacacc aagttattcc
    13141 tgatcgtatt gaagcaggaa cttatatcgc tttagcggca gctattggaa aaggtgttaa
    13201 gatcactaat gttctttatg agcatttaga gagtttcatt gctaagttag aagaaatggg
    13261 tgtccgtatg acggtggaag aggatgctat ctttgttgaa aagcaagaat ccctcaaggc
    13321 tattacaatt aaaacctctc cttaccctgg ctttgccact gatttgcaac agccattaac
    13381 gcccttgttg ttaaaagcag atggtcgtgg aacgattatt gacactatat atgaaaagcg
    13441 cattaaccat gtgccagagt tgatgcgcat gggagcagat atctcagtta ttggtggtca
    13501 gattgtttat caagggccgt ctcgcctgac aggagctcag gtcaaagcca ctgatttacg
    13561 tgccggagcg gctctagtga ctgctggtct aatggctgaa ggcaagacag aaattactaa
    13621 tattgaattt atcttacgtg gctatgctag tatcattgca aaattaacag cactaggagc
    13681 agatattcaa ttaatcgaag actaaagaga agctaacgct tctctttttt tggtactctg
    13741 acctcatgga agaatttctt tggctgataa gttgatgaag aataagcaga ggttgtgctg
    13801 gttcttttta gaaaagaatg ccccaacaga agtggcagtt tgctgaagag gtttggtaaa
    13861 agaaatcgct aacgcgtcat ttttactcat aagcgtttaa ttgtctgatg attagtacta
    13921 aagacgcatt agttatcaga tgatttttct tttaaatctc taaaatagcc ttatttttaa
    13981 aaaaataggg aaaaaactta aaattaggtt gacaaatcat cagatgaata gtaagataaa
    14041 catgtaaaaa ataaaaacgc tttcaagtga agtgttagga tataaggggg gtctctaatg
    14101 gagaaaacga ttgatcaaag ggttgaggaa gttacctata accgagctaa agtttggcaa
    14161 ctggtattgt ttgcgttaaa caatacatct accaacattt acctatttgc tttcatgttt
    14221 atcacctatt tttcaacagg tgttcttggg ctagcagcta tctttgttag ccaaatcatg
    14281 ggttatattc gtatctttga tggttttatt gacccagcta tcggtatcat gattgataaa
    14341 acagatacta agtttggaaa ataccgccca attttaattg ctggtaatat tattacggct
    14401 ctatcgctta ttctattact tgctttaaat aatgttagcg aagggatgcg cttcccactc
    14461 tttattttgg tattgattgt tcacaaaatt ggttattcca tgcaacaaac catcacaaaa
    14521 gcaggtcaaa ctgccttgac gaatgatcca aaacaacgtc ccattttcaa cattgtggat
    14581 gctgtcatgt taacaacctt gatgactggt ggacagtttg tggtttcagg atttttggtt
    14641 cctaaatttg gtaacttcac gttggaattc ttcaatgtgc ttatctatgg aacaatcgtt
    14701 atttcagcta ttttaggatt attggctgtt atcggtatct ggtctaaaga caacaaagaa
    14761 tacttcggtc ttggtgaaaa tactcaaaaa acagaattaa aagactacat gaaagtcatt
    14821 aaaggcaata aacctcttca agtcttgtca attgcagcag cactcgttaa gtttagcgtg
    14881 caattctttg gagactcagt tgtcatggtt atgctttacg gtatcgtgtt tggtaactat
    14941 gccttgtcag gtcaattctc actacttttt gtgattccag gtgttttgat taacattttg
    15001 atttcaaata ttgctcgtaa aaaaggtctt cgtttctcat atgtggcgtc acttcaattt
    15061 ggtttgatcg ccttagctgc ctttggggtt gtcctttact ttggtaaagt tggtgatttg
    15121 agtttaagta acattaacct ttacactatc tgctttatca ttacttacat gttggctcgt
    15181 tacattactc aagcgccggc tggtttggtg ttaacaatgg gggcagatat ttcagactat
    15241 gaaacatctg aatcaggtcg ttatgtgtca gggatgattg gaacaatctt ctcattgaca
    15301 gactctatcg cctcttcatt tgcaccgatg gttgttggag ctgttcttgc aacgattggt
    15361 tttgccaaag ctttcccaac aatggaaaca cctttaacac cagaacttaa aatggcaacc
    15421 attattcttt tagttggtat tccgtttgtc gccttgtcag tcgccttgtt cttgatgaga
    15481 ttctacaaac tggataaaga agaaatggtt cgtatccaag agaaaattca agtcatgaaa
    15541 gctgcaagtt caaaagaacg cgtgaaggat atcgctcgta atgtgccatt gtcagatatg
    15601 gactacgttg atgtaacgaa atatccagtt gataaagatt agtcgtttgg aaggagttga
    15661 gcaagatgtg ctcaactctt tttatttctt gaggcgagag gtttctaatg acgatgaaat
    15721 gacctcttct aagtttttaa aaaaagactc atatcatgta taatgttact atcacgacca
    15781 gtagaggagg ttttggttga aaacagtagt ttctttaggt gaaatcttgt tacgattgtc
    15841 accaccacag tatcaaaccc taactcaggc aaccagtctg gaatgtcaat ttggtgggtc
    15901 ggaattaaat gttttgtcaa cgttggcgca gttagggcat aaggtctctc tcatctcagc
    15961 aataccagcc aatgatttgg gacgaatgac aaagaccttt cttttttctc gtcaaatcag
    16021 tcaagattat attattgaaa aaggtcaacg tttagggatt tattactatc aaaaagggtt
    16081 ttccttacgg gctagtcgcg tgacctatga ccgaaaatat tctgcttttt gggaatcgga
    16141 gttagctgat tatgagttag cagatgtttt tgcgggggct gattggtttc atgtgtctgg
    16201 tattaccccg gctttgaccg ataagcttta tcacttgacc cattatctga tgacaaaggc
    16261 aaaaacagaa ggattaaagg tttcctttga tttaaacttt agggccagcc tttgggactc
    16321 ttttcaagag gctagagaat tattgtcccc tttagttgct ttggcagatg tttgtattgg
    16381 aattgaaccc ttggttttag caggcgatgg ggaaaaagat ttgaaagacc aattgggatt
    16441 gagtcgaccg tatgaagatc aagcgctatt ggaaaatgtt ctttctaagt tggcaaaaac
    16501 ttacggtgtg actagctttg cttttaccca aagggaaatg gggcatacca atgaatacct
    16561 cctgaagggt tatttatatc aagatggtaa tctttaccag acagacaagt ctggcattca
    16621 ggtgttggat cgtgttggta ctggagatgc tttcactgct ggcttgattc atggtatgtt
    16681 acaaaaggaa gtgcctgaca aggccttagc cattgcaatg gcgagtttta aattcaaaca
    16741 cacgattgag ggtgacagca atattattac cccagcagat attgattatt taataactga
    16801 tgcatcgcat gagattaaac gttaacaatg agaaaaaagg aggaaaagat gctataccca
    16861 actttaacca aaacaagaag tttatatgac cttagtggtt tgtggcaatt caaattagga
    16921 gaccataacc cagaagattg tttgtcaaca gaagacgtta tggttgtgcc gacttctttt
    16981 aatgatgtgg tggtcgataa ggataaacgc aattatatcg gtgatttttg gtatgagcgt
    17041 ttggttgatc ttccaaagtt agctaaagat gatgaattgg tcttacgttt tggctcagtg
    17101 actcacaatg ctaccgtgta tgttaatggg cgtgagctgg cttctcataa gggaggattt
    17161 actccttttg aagtcgtggt gccagaggaa ctttaccagg accatcagat cagaatttct
    17221 gtccgtgcca ataatatttt agattacagt acacttcctg tgggtaatta tagcgaagaa
    17281 cgtcaagaag atggcagcat gaaaaaagtg gtcaaagaaa acttcgattt ctttaactat
    17341 gcgggcattc atcgtccagt caaattagtg gtgcgtccta aaacccacat ttcagatatc
    17401 accattacca gtgatttatc agatgatttg aaaacagcag acctttccat tgctgttgaa
    17461 acgtcagcgg ctgttgatgc gattcaggtg actatccttg atgaagaacg tcaagtcgtt
    17521 ggtcaaacga acgacggcca tgtcaaactt gattgcgtgc atctctggga agtcctggat
    17581 gcttaccttt acacagctcg tattgaaatt agtgtagatg gtcaaattgt tgatacttat
    17641 gaagaacctt ttggagtgcg tagtgtacat gtagcggatg gtcagttctt tgtcaatcgt
    17701 aaacttgttt actttaaagg ttttggcaaa cacgaagata cctttatcaa tggtcgtggt
    17761 ctcaatgaag cggctaactt gatggatatg aacttgttga aagagattgg cgctaattcg
    17821 ttccgtacct cacattaccc ttactctgaa gaaatgatgc gccttgcaga ccgtatgggg
    17881 attttggtga ttgacgaaat tccagcggtt ggtttattcc aaaatttcac ggcttccctt
    17941 gacttaacac ctaaagatat gggaacttgg tctgtgatgg aaacaaaaga agcacacgaa
    18001 caggctattc aagaattagt gaaacgcgat aaaaatcacc catctgttgt catgtgggtc
    18061 gtggccaatg aacctgcaag ccatgaagcg ggagcgcatg actattttga acctctggtg
    18121 actctctaca aagaattgga cccacaaaaa cgtccagtga cattggtcaa tattttaatg
    18181 gcgacaccag accgagatga agttatggat ttggttgatg tgatttgttt gaaccgttat
    18241 tacgcttggt atgtggatca cggtgatctg gctaagggag aagctggcct acgcaaggaa
    18301 ttgttagaat ggcagagcaa gttcccagac aaacccattt tgatgactga gtatggggca
    18361 gataccttgc ctggcttgca ttcgatgtgg gatattccat atacggaaga attccaatgt
    18421 gatttttatg cgatgacgca tggagttttt gatgagattc ctaaccttgt tggtgaacaa
    18481 gtttggaatt ttgcagactt tgagaccaat ttaatgattt tccgtgtgca aggtaaccat
    18541 aaaggactct tctcgcggaa ccgtcagcct aaacaagtgg tcagagaatt taaaaaacgt
    18601 tggacagcta ttcctaacta caattacaag aaaaaatagt gtaaaatagg agacagtaga
    18661 aacaggaaga ggacgatacg atggctagac cattagttga aaaaacagca gaacgtttat
    18721 tacagcttat tttagaacgt ggatatgacg taggggctaa gctccctaat gagtacgaat
    18781 tagctcaaga cttggatgta ggacgtagca ctatccgaga ggctgtcaga agtttagcaa
    18841 cccgcaatgt cttagaagtg agacagggtt ctggaaccta tgtgagttct aaaaaagggg
    18901 tatcagaaga cccgcttggt ttttcattgg tgaaagatac ggcaaaattg acagcagatt
    18961 tgtttgaatt acggttgtta ttagaacccc ggattgctgc cttagctgct cagaatgcga
    19021 caccgaaaga gattaaagag cttgaaaaga ttgtcgtaga gattgaagaa gctgtaacag
    19081 tgggtgatcc taagcattta cagttagatg tgaccttcca ttcgatgatg gccaaattta
    19141 gtggtaatat tgcgatggac agtttattgc ctgtcattaa caagtcaatt catctgataa
    19201 atgctaacta cacaaatcgt cagatgaaag aagattcttt acaagcccat cgcgacattt
    19261 tgacagctat taaggagaga aaccctatcg cagcgcatga tgctatgctt ttacatatta
    19321 tgaccgtaaa acgaacggct ttcttgcctt aataaatatc ggcgaagaac aaacagcaat
    19381 tgtgataatt gctgtttttt tagacttttt tcttgacaag tcatcagacg atttgtataa
    19441 taataaaaag ataacggttt catagaggag gaaaaatgga tgttaaccca attaaaagag
    19501 aattactttt tcgctgttgt gcgtggaaaa accgcagaag atgcgattga aatcgctaaa
    19561 tatgcggttt taggtggaat tcgtaatatt gaaattactt actccactcc aaatgcttct
    19621 gaggtcatta gcaaattatc agacgacttt aaagatgaca caagtgttgt tattggagct
    19681 ggtacggtaa tgactactga actcgcaaaa gaagcgattg cagcaggtgc caaattctta
    19741 gtaagcccgc atttttccaa agaaatcgct gatattgcta accaagaagg caatctttac
    19801 ttcccaggtt gcgccacagc aactgaaatt gtgactgcca tgaatgcttc ttgtcctatt
    19861 attaaggttt tcccaggagg ggttgttggc ccaagtttta tcaaagatat tcacggtcca
    19921 atccctgagg ttgatttgat gccttctgga ggggtttcag ttgataacgt gaaagagtgg
    19981 aaaaatgctg gagcagtcgc tgtaggtgtt ggttccgccc ttgctagtaa ggtggcgaca
    20041 gagggttatt acagtgtcac tcggattgcg caatcatttg tatcagccct tgactaaatg
    20101 tggaagttat cacagaggag acgaacatga catttaacga tgcgaacttt atgttaaaaa
    20161 atgaagctgc taaaaaactg tatcaacaga ttcaagatca accgattttt gactatcact
    20221 gtcacttaga tccaaaagaa atttttgaag ataaggtcta tgataatatt gttgacttat
    20281 ggttaggcgg ggatcactac aagtggcgat tgatgcgtgc taatggcatc tcagaagaag
    20341 aaattactgg ttcagcgtct aaacttgata aatttaaagc ttttgcaaga accttagaac
    20401 gttcctacgg taaccctgtt tatcattggt cagctatgga gttgaaaaat gtttttggtg
    20461 tggaagagct gttgacacag gacaatgcgg aagagattta ccatcgtttg aatgcttatc
    20521 tggttgacca taagattagc ccgcgcaaat taattgcaga cagcaaggtg acttttattg
    20581 gaactactga tcaccctctt gatgacctcg aatggcatca acaacttgcg gacgatagca
    20641 ctttcgagac agttatagca ccaaccttta gaccagatga ggcttttgta gagcaccgca
    20701 attttgcaca gtttgtggag cgtttaggaa cttcaactgg caaccgcatt acagacttta
    20761 cgacgtttat ggcagccatg gagaaacgga ttgcttactt tgctgaaaaa ggctgcaagg
    20821 caagtgatat cagctttaca gaaattgttt ttgaaaaggc tacaccagaa ggtttaaatc
    20881 agattatggc ccaggtgcta gcgggtgata caccaagcca gcttgacatc aaccaatggc
    20941 agactgctgt tttttgcaga cctttgtcga ttgtacaagc aatatggttt tgtaacccaa
    21001 gttgattttg gtgctctgcg caacaaccat tctggtattt tcaaacgttt aggagcagat
    21061 ataggggtgg attctctagg agaccaaaca gccttaacca gcaatatgaa ccgtttattg
    21121 gataacttgg tgcagactga tagcctacca aaaatgatct ggtataactt aaacccaagt
    21181 tacaacattg cagtagctaa cactttggct aattttcaag ccaatgaagc cggtgtggca
    21241 agttaccttc aatttggagc aggctggtgg tttgcagata ccaaacttgg aatgattagc
    21301 cagatgaatg ctcttgctga acaaggaatg cttgctaatt ttgtaggcat gttaacggat
    21361 tctcgttcgt tcttgtctta tcaacgccat gattacttca gacgtatttt agctacctac
    21421 ctaggggaat ggattgaaga aggggaagtc ccagaagatt atcaagccat tggtcagatg
    21481 gctaaagaca tcgcttacga caacactgtt cgctatttta attaaaactg tatcaatata
    21541 gaagtgaaat cttctcaata aatgaaaatc gtgtggaaat gaaaggagaa aggagcctat
    21601 cagctctagg taattgtata tgaaaatgtc atttcgctgg tatgggaaac atgacccagt
    21661 cagcttagaa gaaatcaaag ccatcccagg tatgcaggga attgttacgg cgatttacga
    21721 tgttccggtt ggtcaagctt ggccacttga gaacattctt gccttgaaga aaatggtcga
    21781 agatgctggt ttagagattt cagttattga atctatccca gttcatgaag acatcaaaca
    21841 aggcaagcca acgcgtgatg cattgattga aaactataaa acctctatta tcaacgttgg
    21901 taaagcaggt attccggtgg tttgttacaa ttttatgcca gtttttgact ggacacgttc
    21961 agatcttaac catccactac cggacggatc cacatcgctt gctttcttaa aagctaattt
    22021 agagggtgtt gatccagtag ctgacgattt gaatctacca ggttgggatt cttcttattc
    22081 taaggaagaa atgaaagcca tcatcgagaa ttaccgcaac aatatctctg aagaagattt
    22141 atgggcaaat ctagactact tcatcaaagc tattatgcca accgcagaag cagctggtgt
    22201 gaaaatggcg atccaccctg atgacccacc atatggtatc tttggtttgc cacgtattat
    22261 aacaggtcaa gatgcagttg agcgcttctt gaatctctat gattctccaa acaatgggat
    22321 taccatgtgt gtagggtcgt atgcttctga tcctaaaaat gatgtgattg ccatgactga
    22381 gtacgctctt aaacgtaacc gtatcaattt tatgcacacg cgtaacgtga cagccggcgc
    22441 ttggggcttc caagaaacag cacatctgtc acaagctggt gatattgata tgaatgctgt
    22501 tgtcaaatta ttggttgact atgattagaa aggggctctt cgtccagacc atggtcgtcg
    22561 tatctgggga gatcaaacca aaacacctgg ttatggccta tatgatcgtg ctttgggagc
    22621 tacttacttt aatggtcttt atgaggctaa tatgcgtgct gctggtaaga caccagactt
    22681 tggcattact gtcaaaacag ttggcgacaa ataagaaaga ggaataacat gactagagta
    22741 attgatttta aagataaggt tgtgattgta acaggtgctg gtggcgtgct ttgtggctac
    22801 atggccaaag agtttgctaa agcaggtgcc aaggtagctc ttttagattt gaaccaagaa
    22861 gcagcacaag cttttgtcac tgagattgaa gcagaaggtg gtgtggctaa agcttacaaa
    22921 gcaaatgtcc tctcaaaaga gaatttagaa gaggtacgtc aggctgtttt agctgatttt
    22981 ggtccagcag acattttggt caatggagct ggtggaaaca gtccaaaagc gactactggt
    23041 aatgaatttc atgaggttgg tttgcctgaa gagaccaaaa ctttctttga tttagataaa
    23101 gcgggaatta gttttgtctt taacttgaac tacctaggaa cattgcttcc tacgcaagtt
    23161 ttcgctcaag acatggttgg gcgaaaaggg gctaacatca ttaacatttc atcgatgaat
    23221 gctttcacac cattgacaaa aatcccagct tattcaggag ctaaggcagc tatttccaac
    23281 tttacgcaat ggttagctgt tcacttctca aaagtgggaa tccgttgtaa tgctatcgcc
    23341 ccaggtttct tggtgaccaa ccaaaaccgt ggccttttgt tcactgaaga ggggcaacca
    23401 acagcacgtg ctgaaaaaat ccttaacaat acgccaatgg gacgtttcgg agaagcagaa
    23461 gaattgattg gtggtctctt cttcctagct gatgaacaat cagcaagctt cgttaacggc
    23521 gttgttctcc cgattgatgg aggctttgca gcttactctg gcgtttaatc cttagtaact
    23581 aactcactca agaaaatgtg atgccttggg tgagtttttg tttagagact agttgctatc
    23641 atcataagtg atatgatgac ttggtgagcg ttagaatgat cgttaagaga gaataatttg
    23701 gcgacaactt ggatattcat cttaaaataa aggataacaa gaaaaatgaa cctaatgtta
    23761 gttttctaat tgacagcgtt ttctggctat gttacaataa agtcatcaga tgaattataa
    23821 ttaattggag gaaaacatga cgactaaaaa ggaagccttt gtcttctgcg tagactcaga
    23881 tggctgtgcg atggatacga tgacctataa gcatcagcta ttctttggtc caattgctgc
    23941 agatgtcttt ggggtgacaa atagagaagc atttttaaaa gaatggaacc gtgttaacct
    24001 gtactcacgc acgcgtgggg ttaatcgttt tgttgggctt gtcatgggac ttgactatgc
    24061 aggattgaaa ggcattgacc gtttgaaaga ttgggttgcc aacaccaaat ccctctccaa
    24121 tgcttcactg gaagcagagc ttgctaaaga agctagtgat gaccttcaaa aagctttgga
    24181 atggtctaat gaagtgaacc gtcaaatcaa agcttatgaa ggtgaggctt tggcttttcc
    24241 aggagctgaa tcaggccttg agaaattgca tcagctaggg aaagtattcg ttgtgagttc
    24301 agccaataaa gaagctgtcg aagaagagtg gcacgatcaa ggactaatgg cacatgttga
    24361 tgacttgtat tgccaagact gtggtaaaaa agaggatgtt attgcagagc ttcttcaaaa
    24421 agggtatcaa aaagaacgcc tcatgatggt tggcgattct ccaggagatt tggccgcagc
    24481 agagcaaaat caagtggctt tcttccctat attagtcggc aaagaggcgc aatcttgggc
    24541 tgacttaaaa gaagttgtgg cagatgcctt tgtgacagga gaactgtctg acctagattt
    24601 taatagcttg aaagaaacct tctggcataa cttagattag atcaatagca gagctactaa
    24661 ttagattgac tagtccaaaa atatgagaaa agaagtaagt agagggattt ggtaagaagg
    24721 tattaaaaaa ccgagaccct cttgaaagga aaagaaatga cacatttagt agatttacgc
    24781 aaaaaacctt atcaccttga tgaccaagcc atcgcttggg tagaagaaac cattgcctcc
    24841 atgtccttgg aggagaaaat tgggcagtta tttgttaaca tgggagctag tcgtaccgaa
    24901 gagtatttga cccaagtgct taatgattat aaaattgcag cggttcgcta caataaaggc
    24961 atggccgacg aagtttggga acaaaactac attttacaaa ccaaatcaaa aattccactc
    25021 ttaattgctg ctaacaccga agcgggtggc gatggcgcag ttaatgatgg tacaaaggtt
    25081 ggggacgaga ttaaggttgc cgcaacaaat gatccaaagt acgcttacga aatggggcgc
    25141 attgcaggga ttgaagcagc agcagttggc tgtaacgctt cctttgctcc tattgttgac
    25201 ctgacacgca actggtgcaa cccaattatt gctagccgta actggggctc aaacgtggac
    25261 caaatcatta ccttttctaa agagtacatg agagggatta tggagcatgg cattatgcca
    25321 tttgccaaac atttcccagg ggacgggatt gatgaacgtg accatcacct ctcttttgca
    25381 tcaaacccaa tgacaaaaga cgagtggatg tctaccttcg gtcgtatcta tggagaactg
    25441 acagaagctg gtcttccagg tattatggcg ggtcatatcc acttgccaaa tgtggagaaa
    25501 gaaatgcatc ctgagcgtga attggatgac atgctaccag cttcactcaa caaaacgttg
    25561 ttggatgaat tgctgcgcgg ggagcttggt tacaatgggg ctattgtcac agatgcttct
    25621 cacatggttg ccatgacagc ttctatgcca cgtcgtttga tgctaccaac ggctgtggaa
    25681 gcgggatgtg acctcttctt attctttaat gatccagatg aagacttgca atggatgaaa
    25741 gaaggctatg aggctggtat tttgacagaa gaacgactcc atgatgccct tcgccgtacg
    25801 cttggcttga aagcacgtct aggacttcat ttatatgaag gtcgtcgtga agagattatg
    25861 ctaccaaaag aagaagcgat gacactgatt aataccaaag aagctcaagc cattgctgac
    25921 gaggtagctg acaaagccat tactcttgtt aaagataagc aaaaggatat tttcccagtc
    25981 acgcctgaac gctacaaacg cattttgatt gtcaatgtag aaggtcacaa aggtggtttt
    26041 ggtgcgatga ttgcagggca gaaaaaacga gcatccgatg tggtcaaaga attgctagaa
    26101 gcgcgtggcc atgacgtcac agtgtgggag tcaacggaag aacgtatcat gcaattgcca
    26161 gaaagtgaac gtgccgcagc cattgccaat atttatgccc aaaaacaacc gatttccaat
    26221 ttgacagacc attacgattt aatccttaac cttgttgatg tcaactctgg tggaacagtt
    26281 cagcgtattg tctggccagc agctaagggt acaccggatc agccatttta tgtgcatgaa
    26341 atcccaacaa ttgtggtatc agttcagcat gcctttgccc tagcagacat gccacaagta
    26401 ggaacttata tcaatgctta cgacggtaag gacaatacca tgaaagcttt ggtggagaaa
    26461 ttggctggcg aatccaactt caccggtgtt tcaccagtag atgctttctg tggccttatt
    26521 gacacgcatt tatggagaga atattaggaa agtcattgtt tgactaagtg agagtagtaa
    26581 aggacgtaag gcatgccaaa aacagttgat ttaaggcaaa aaccctatca gcttgacgat
    26641 cagcaagttg ctttggtaga agatacctta gcagggttaa gtgatgaaga gaaaattgga
    26701 caactttttg taaatctttt tttctttggt gaagatgcgt ttagaagcaa tcaattaagc
    26761 aacaaatata tcttggcaaa ctatcatatc ggtggcgctc gttaaatgaa cggcacctct
    26821 gaacagatgc aaggacttct aaatgagttg cagagccaca gcaaggtcgc agccagttgt
    26881 gactctggtg gagatagtgc ggtaaaaggt gggacctaca ttgcttcacg agctcaatcc
    26941 gaggcaagcc atgacctttg gcttgtttac tatgcagggc ttgtttctgc tagggaagaa
    27001 acaagctcta ggcgttaata ttaactttga tggcagtctt cttaaatctc ctgaagacct
    27061 tgctatcact ggctgtgaag agcattagtt tggcaaaaag aagctgcaca aaaaaccatt
    27121 atccttttaa aagatactca agaaaacctc ccgattagcc cagaaactca cagacgtatt
    27181 cgcctctatt gcttagaggg gggaaaggag gaattatgag tgccaatgat caagtggtcg
    27241 gaaaccttaa acaggcccta gaggcaagag gttacgaggt gacgattaat gatggcacga
    27301 ctcgcgtcaa gggagagatc ttggcctatc gtaatgacgt ggatttggcc ttgatggtgg
    27361 tcaatgttgt tgactatgaa gctcaaaata attaccgtat ccaatgggag actgccatgt
    27421 ttaatgaggt gccttggtac gtttatgaag tttcaactgt ctttatttcc cttaattaca
    27481 agacgcatct gcacgatgcc actatggtca aaacagcgat taatgcttat caccataata
    27541 ctaatacgat tgcagccctt ttggattgtc tagaaggcaa agcagctttt aaaggccaac
    27601 ctaatgagct agtttgggcc aataagtggc aagccaagct gtaataaaga tgatgatgga
    27661 aaagttagaa agatcttcta actttttttg tttctcaaaa acg
//
LOCUS       DAUCWU010000014        24819 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUCWU010000014 DAUCWU010000000
VERSION     DAUCWU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.014"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(23..1966)
                     /gene="thrS"
                     /locus_tag="VGY24_000588"
     CDS             complement(23..1966)
                     /gene="thrS"
                     /locus_tag="VGY24_000588"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ4283593.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(2388..3722)
                     /locus_tag="VGY24_000589"
     CDS             complement(2388..3722)
                     /locus_tag="VGY24_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ4283594.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHFAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLR
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(3724..4722)
                     /locus_tag="VGY24_000590"
     CDS             complement(3724..4722)
                     /locus_tag="VGY24_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ4283595.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(4853..5854)
                     /gene="ccpA"
                     /locus_tag="VGY24_000591"
     CDS             complement(4853..5854)
                     /gene="ccpA"
                     /locus_tag="VGY24_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ4283596.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            6028..7113
                     /locus_tag="VGY24_000592"
     CDS             6028..7113
                     /locus_tag="VGY24_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ4283597.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLIKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHQMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKLGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            7162..7827
                     /locus_tag="VGY24_000593"
     CDS             7162..7827
                     /locus_tag="VGY24_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ4283598.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            7838..8215
                     /locus_tag="VGY24_000594"
     CDS             7838..8215
                     /locus_tag="VGY24_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ4283599.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            8360..9286
                     /locus_tag="VGY24_000595"
     CDS             8360..9286
                     /locus_tag="VGY24_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4283600.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNCITVMDVDLQDPP
                     ELLPIMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKHGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFDDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            9497..10180
                     /locus_tag="VGY24_000596"
     CDS             9497..10180
                     /locus_tag="VGY24_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ4283601.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQSLTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            10180..11106
                     /locus_tag="VGY24_000597"
     CDS             10180..11106
                     /locus_tag="VGY24_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEQ4283602.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            11156..11803
                     /gene="pcp"
                     /locus_tag="VGY24_000598"
     CDS             11156..11803
                     /gene="pcp"
                     /locus_tag="VGY24_000598"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ4283603.1"
                     /translation="MKILVTGFDPFDGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(11919..12638)
                     /locus_tag="VGY24_000599"
     CDS             complement(11919..12638)
                     /locus_tag="VGY24_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ4283604.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(12644..13111)
                     /gene="smpB"
                     /locus_tag="VGY24_000600"
     CDS             complement(12644..13111)
                     /gene="smpB"
                     /locus_tag="VGY24_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ4283605.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(13114..15447)
                     /gene="rnr"
                     /locus_tag="VGY24_000601"
     CDS             complement(13114..15447)
                     /gene="rnr"
                     /locus_tag="VGY24_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ4283606.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKRPADRLKGTAAEAKIVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSSLDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINPQGYVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILETMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKVEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYNQPSQEKRDHFAQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(15541..15777)
                     /gene="secG"
                     /locus_tag="VGY24_000602"
     CDS             complement(15541..15777)
                     /gene="secG"
                     /locus_tag="VGY24_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ4283607.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(15823..15969)
                     /gene="rpmG"
                     /locus_tag="VGY24_000603"
     CDS             complement(15823..15969)
                     /gene="rpmG"
                     /locus_tag="VGY24_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4283608.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(15966..17159)
                     /locus_tag="VGY24_000604"
                     /pseudo
     CDS             complement(15966..17159)
                     /locus_tag="VGY24_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            complement(17281..18783)
                     /locus_tag="VGY24_000605"
     CDS             complement(17281..18783)
                     /locus_tag="VGY24_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ4283609.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALEVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSDI
                     ESQQLVQSSPFASKYSQSIDRDSAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKE
                     QAAAEKTSRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(18973..19566)
                     /locus_tag="VGY24_000606"
     CDS             complement(18973..19566)
                     /locus_tag="VGY24_000606"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ4283610.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            complement(19563..20390)
                     /gene="mutM"
                     /locus_tag="VGY24_000607"
     CDS             complement(19563..20390)
                     /gene="mutM"
                     /locus_tag="VGY24_000607"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ4283611.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(20562..21428)
                     /gene="rgg2"
                     /locus_tag="VGY24_000608"
     CDS             complement(20562..21428)
                     /gene="rgg2"
                     /locus_tag="VGY24_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ4283612.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            21517..21585
                     /gene="shp2"
                     /locus_tag="VGY24_000609"
     CDS             21517..21585
                     /gene="shp2"
                     /locus_tag="VGY24_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ4283613.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            22499..22768
                     /gene="stcA"
                     /locus_tag="VGY24_000610"
     CDS             22499..22768
                     /gene="stcA"
                     /locus_tag="VGY24_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ4283614.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(23116..23514)
                     /locus_tag="VGY24_000611"
     CDS             complement(23116..23514)
                     /locus_tag="VGY24_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ4283615.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(23607..24677)
                     /locus_tag="VGY24_000612"
     CDS             complement(23607..24677)
                     /locus_tag="VGY24_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283616.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSGTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMSLMKDIRIALDGSDKSLTAQIV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIK
                     VSINPKGRQATITYGDGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT         7323 a         5387 c         4513 g         7596 t
ORIGIN      
        1 aaggacctct ttttgccatt gattaagctt gtgcatctgg acgtgacttg cgagcaatgt
       61 ctgccaagat gttttccaca aattcttcca cagattcagt gtgagtcgcc ttactaccat
      121 agcggcgaac attgactgat ttgtcttcca tttctttgtc cccaacaatt aattggtaag
      181 gaattttgct ggtttgagac gcacgaatct tgtactgcat tttttcattg cggtcatcga
      241 catctgcacg aacaccacgg tcacgaaggg tttttgctac ttcccaagca tagtcaatgt
      301 gggcttcatt tgaaattgga atcacggtta cttggtgagg tgccaaccaa gttgggaaag
      361 cacccttgta agtttcaata aggatagcag tgaaacgttc catagttgag ataacaccac
      421 ggtgaatcat gactggacgg tgttcttcgc catctgcacc aatgtattta aggtcaaagc
      481 gttctggcaa caagaagtcc aactgaatgg ttgatagggt ttcttcgtta ccaagagctg
      541 ttttcacctg aatatcaagc ttaggcccat aaaaggctgc ttcaccttct gcttcaaagt
      601 aatctacacc catctcatca agtgcagctt taagcatact ttgagcattt tcccacatct
      661 catcattgtc ataatattta tgagtatcgt tagggtctcg gtatgacaaa cgaaaacgat
      721 aatcagtcaa gttgaagtca gcataaacat caatgattaa ttgaagggca cgttgaaatt
      781 cttcctggat ttgctctggt gttacgaaaa tgtggccatc attcaaggtc atttcacgta
      841 ctcgttgaag tcctgataga gcacctgatt tttcgtaacg gtgcatcatg ccaagctcgg
      901 caatacgaat aggcaattca cgataagaac gcacatgatt tttatacact tgaatgtggt
      961 gtgggcagtt cattggacga agaacaaact cctcaccatc tcccatatcc attacaggga
     1021 acatatcttc ttgataatga tcccaatgac ctgatgtctt gtacaattct acagaagcaa
     1081 gtggtggtgt gtaaacgtgt tggtagcctg atgctaattc tttatctgtg atgtagcgct
     1141 caagtgtacg acgaatagtc gctccatctg gtaaccaaaa cggcaagcct tgaccaactt
     1201 cttgactaat catgaacaag tcaagctctt taccaagctt acggtgatca cgttctttgg
     1261 cttcttctag acgagtcaag taagctttaa ggtctttttt atcaaaccaa gctgttccat
     1321 agatacgttg catcatgtta ttgtcgctgt taccacgcca gtaagcaccc gccacattga
     1381 gcaagtggaa gacttggata cggcctgttg atggcacatg agggccacgg caaagatcca
     1441 caaattcacc ttgacggtaa accgtcaagc cagcaccagc atgctcatta atcaattcta
     1501 ccttgtaagg atcatctttg aacaattcca aagcttcttc tttagtcact tcttcacgga
     1561 tacatgggta attttctgtc acgatttttt gcatttcagc ttcgatacgt ggcaaatctt
     1621 cgtttgaaat ttggccttca gcattatctg tatcatagta aaagccttct gcaatggcag
     1681 ggcccacacc caaatgaaga tttgggaaca agcgttttgc agcttgggca aataaatggg
     1741 ctgcagagtg gcgaagcacc tcataagcat ctttgtgatc aggtgtcacg atttcaatac
     1801 tgccatcttc ttcaatggca cgagtggtgt caatgagttg atcattgaac ttaccagcta
     1861 aagctttttt agccaatgat ttgctgatgg actcagcaat gtcaaacgtt gttacgccag
     1921 attcaaattc acgcacggcg ccatctggaa aagtaatctt aatcatttgc tttctccttt
     1981 aagtcagtta agatttccac taaaacatgg atagtaagac aaattaggga caagaggtta
     2041 gtgaatagct caataccacc acgtagaaga acaatacctt attcacgaat ctaccctccc
     2101 tagtccttaa aaacaaggct aatcccctcc aaaacaaaaa aactgaccgc atcccaaagg
     2161 acgcaatcag cgtggttcca ccttaattta tgcaggacaa aaaacatgcc ttgcacctct
     2221 agtgacgata agggggccac ccttttatgt ttgcataaaa accaggaagg agtatcagat
     2281 tagctatcct tgtgctcttt ccaccaacca gcactcgctt aaaagattct gtccaatcta
     2341 acttgtcttc aagattatta tagcaagttt tcaaaaattt acaagcctta atccagaaaa
     2401 tctttgatag catggaccag cttgatagga gccttgacta cttttactga ggtgatagct
     2461 gtgaccttag cagcacgttt aggcaagtga atagcacctt gcactaattt tagagaacta
     2521 cttttaccag aatgattaag ataaagtctc agcttttgag cttctttgct atttctagca
     2581 attaacgtat ctaaatagaa cgtgtaaata gattttccaa aatgctgtgc tgagatttca
     2641 taacgttttt ttgctaataa ccgcttatcc ataactggtg tttttagtat ggcatcaata
     2701 atagcatcag ttaaatctgt ttcagcgtaa taaagagtgc caaacatttt atcagtcacc
     2761 acatcatcta aataaggatt gccatgagca ataataggag tgccactagc caaactttca
     2821 atataggtca agccctgagt ctcactagtt gatgctgaga taaagaaatc acaagcctta
     2881 tagtacagag caaccttatc atgaggcacc atgcctgtaa aggtcacgtg tttgtcaacc
     2941 tctaactgca tcgcaaagtg tttcaaatct tgcaaatagg ggccatctcc tacaataata
     3001 agctttatct tggcattttc agccaaaata gctggcatct gattgatgat agcttgaata
     3061 tttttttcat aagaaatccg tgacaaactc aataacatgg tttcatcacc agcaatgccc
     3121 aattctgctt ttaagttggt tacttcttct gctgtgatgt catcacgaat atatttttcc
     3181 aaaggaatgc ctgttgggat aacccgctta gggatagtca cttcgtaacc ttcaagaaga
     3241 ttgaggacaa tcctacttgg gcagataacc ccatccaaat ccttcaaata gcccctaaga
     3301 agaggtttga ccatacttgg tcgaatgatt tttccgttgg caatataact cacgtagtcc
     3361 tcatattggg tatgataagt atggacaaca ggaattcgca aagctttgcc tatcattttc
     3421 cctaataagc ctaagctaaa ctcagtttgc gtatgaataa tatcaagatt atagtgtttt
     3481 gcaattttgt atgacgaaat gaggccacga taaaccacac gtctatccgt aaatgacaca
     3541 aaaggaacac ttggcagtcg aataatggtc gggtcttcaa agcgtttgac atctctatca
     3601 gtagtggtga aaatataaac ttcgtgacct tctttttcta gctcttcttt taacgtacga
     3661 atactagtag cgactcctga aacttgtgga aaataggtat ctgtgaatag acctacacgc
     3721 atcttataac tccattactt ttttatagac atctaccaag gcatggccaa ccgtttctag
     3781 gcgacgactc tgggcaacct tgtaaccagc ttcaactttg ttgctggcac cagaaaagac
     3841 ttttttcaga gcttctataa aacctggtat atcggttgct aattccgcac tactttgatc
     3901 aacccatccg tagtaaactg gtatatcacg taaaacaagg tgctggcgac tggccaaggc
     3961 ttctaagaca acaatgcctt ctgtttcttc acgacttgga aagaaaaagg catctgcacc
     4021 agtcatggca ccttcataaa catccccttt aatgtatcct gggaaaataa gatttttcgg
     4081 gtggttacca ttgaccattt ggcgaacttg agcaggaatg acccatttgt tggtctcgcc
     4141 aaaccagata aaacgaacat ctggcatagc ttgggcaact ttgacaaagt catcaattcc
     4201 tttcctcaga aaaaataatc ctgctcccat aaccactttt tcaccctctt taatgtcaaa
     4261 gtagcgacga aaagctgctt cctttttagg atctgctccg tactgctcca aatcaatccc
     4321 atttgacact gcaaaaatag gtgacttcac tccataactc tcaatcaaag acttagaata
     4381 tagggtaggg gtaatgatag catctgcctt attgtaaaag tgacaaaggt attttttaaa
     4441 ccaaggagat aatagatttg aaaaaataaa agaatttcta aaatcttctt ctgtagaatg
     4501 cccatgcatg ataaccttct taccagcttt ttgtgctttt atcatcagca gccaactttt
     4561 taaaccatag gtattgagat gaaccaaatc ataagtttct cttggattag tcgtaaaatg
     4621 ttgaccaaca agtgacaagg ctttagcctg atgcttaatc gctcgaccaa ttcctgattt
     4681 tcttagataa ttttctgctt ctaaatacaa taagactttc ataagttcta ttatagctca
     4741 ttttatagta tttttcacta agacatgcaa aaatcagccc taaggctgat tttagtattg
     4801 aagaaacagt ctattgtgat acttgttgtt agatggtgct cataagtcac agttacttag
     4861 ttgtcccacg ttttttaata ccatgattca aaagaatttc tttttcttcc aactcttctt
     4921 tgttcatgat tttagtcaac atccgcatgc taacagcacc taagtcataa acaggttgac
     4981 tgatagaact caaattggga cgagtgtatt gaaccactgg tgaatcgttg ctggtgatga
     5041 tttcaaagtc ctctggtacg cgtttacctg cttcaaacaa gccgtttaaa aggcccgctg
     5101 ccaattcatc ttcagccaca taagccgctg ttgctcctga gttaatgacg cgctgcgcca
     5161 attcaaatcc ttctttataa gaatagttcg cttcaaaaac aagaccttct ttgaagtcaa
     5221 gcttattgtg cttcaaccct tctttgtaac ctgctaagcg cactttacca ttgatatcat
     5281 caatgagtgg tcctgacaca aaagcgatac acttatgatt ttcagctaaa atatcaacaa
     5341 cgtttgacac cgcagctcta tagtcaatgt tgacgcttgg taattgatga tcaagatcta
     5401 cagttcctgc caaaacaact ggagtacgag agcgtgaaaa ctcggcccgg attttttctg
     5461 tcaagtgatg ccccataaaa ataataccat ccacttgctt ggcaaaaagg gtattgacaa
     5521 cattaacctc cttgtcatca tcttcatcac tggaggcaag cacaatatta tatttgtaca
     5581 tagcggcgat atcgtcaata cccttagcta agatagaaaa ataactattt gcaatatttg
     5641 gaatcacaac gccaacggtt gttgtctttt tgctggcgag accacgcgcg acagcattgg
     5701 gacgatagtc aaggcgatca ataacttcta aaactttctt acgtgtattt tccttaacat
     5761 ttttattgcc attaacaaca cgactaacgg ttgccattga aacaccagct tcacgggcaa
     5821 catcataaat tgtaatggta tcatctgtat tcatttttta tcttcctttc tatttgaaaa
     5881 tgacgctttc aaaaagtatt ttagcatgat ttgtaaccac tttcaagtgt tataggttaa
     5941 tttttgaaaa agttttcaaa cattaactcg aagcttgatt ttttaagctt tttttgaaaa
     6001 aatagtaagt agaaagaagg ttagcctatg acaaaattag accaaatacg tttgtatctt
     6061 gaccaaaagg gagctgaatt ggcgattttc tcagatcctg ttacgattaa ctatttaact
     6121 ggattcttct gtgacccaca tgaaagacaa ctgttcttgt ttgtctacca tgatttagct
     6181 cctgtgcttt ttgtacctgc ccttgaggtg gccagagcta gccaggccat atcatttcct
     6241 gtgtttggtt atgttgactc tgaaaatcct tgggaaaaga tcaaggccgt tttaccaaat
     6301 acagcagcca aaaccattta tgctgaattt gaccacctta acgtcaataa gttccatggc
     6361 ttgcagacta tcttttcagg gcaatttaac aatctaaccc cttatgtcca aggcatgcgc
     6421 ctcatcaaat cagctgatga aatcaataaa atgatgattg ccgggcagtt cgctgataag
     6481 gctgttcaag ttggttttga caatatttcc ttagatgcta ccgaaacaga cgtcatcgct
     6541 caaattgagt ttgaaatgaa aaaacaaggc attcatcaaa tgagctttga cactatggtc
     6601 ctaactggga ataatgcagc caatccccat ggcattcctg gaacaaataa cattgaaaac
     6661 aatgccctac ttctctttga cttaggtgtt gagaccttag gctacaccag tgatatgacc
     6721 agaactgtgg ccgttggtca gccagaccaa tttaaaatcg atatctacaa tctctgccta
     6781 gaggcacaac tagcggctat tgactttatc aaactaggtg taacagctgc tcaagtagat
     6841 gctgctgctc gtcaagtgat tgaaaaggct ggatatggtg agtacttcaa ccaccgcttg
     6901 ggacatggta tcggcatgga tgtccacgaa tttccctcca ttatggctgg caatgacttg
     6961 gtccttgaag aaggtatgtg cttctctgtt gaaccaggca tttacattcc aggaaaggtc
     7021 ggtgtgcgca tcgaagactg tggccatgtc actaaaaatg gctttgaagt ctttacccat
     7081 actccaaaag agttgcttta ttttgaaggc taagccccta ccatctggtt aaacaaaccc
     7141 tcgcgaaagg aaggtattcg tatggatcaa accattcatc atcaaataca gcaagcacta
     7201 cactttagaa cagccgttcg tgtttataag gaagaaaaga tttctgatga ggatttagcc
     7261 cttatccttg atgctgcttg gttaagccct tcttctgttg gcttagaagg ctggcgcttt
     7321 gtcgttttag acaacaagcc tattaaagaa gaaatcaagc cttttgcctg gggggcccag
     7381 tatcaactgg agacagctag tcactttatt cttttaatag ccgaaaaaca tgcaagatac
     7441 gatagccctg ctatcaaaaa tagcctttta cggcgtggta ttaaagaagg tgatggtctc
     7501 aacagccgcc taaaactcta tgaatctttc caaaaagagg acatggatat ggcagataat
     7561 cctcgggcac tctttgattg gacagctaaa caaacctata tcgctcttgg taatatgatg
     7621 atgacagctg ctcttttggg cattgatact tgccctattg aaggctttca ttatgataag
     7681 gtcaatcata tcctagctaa gcataatgtg attgatttag aaaaagaagg cattgctagc
     7741 atgttatccc taggctatcg cctgcgagat cccaaacatg ctcaagttcg taagcctaaa
     7801 gaagaagtga tttcagtcgt taaataagga gattaaaatg aaagcattac atacctgtat
     7861 ccgcgtcaaa gaccttgacc agtcggttgc tttttacaca agtgccttcc cttttaaaga
     7921 aaactatcgt aaagattttc cagatagtca atttactctt gtttatctgg ctttagaagg
     7981 agaaagttat gaattagaat taacctacaa ttatggtcat ggcgactacg acttaggaaa
     8041 tggctatggt catattgccc ttggctctga acattttgaa gcagaccata aaaaacatcg
     8101 acaagcaggt tttccagtta ccgatatcaa agaactggct gacaaatctg cccgctatta
     8161 tttcattcag gatccagatg gctacaaaat tgaagtcatt gatttaaaca actaatgcta
     8221 ttaacaacag aagctttaaa gcttctgttg ttttattttt taacctgacg attgggacaa
     8281 tctcattatt aacagagttt tgaaatatct cagcttttgt gttacaattg ccctaaaatt
     8341 ccatttgaaa gaaaagtcta tgacactact atctatcatt gtcccctgct ttaatgaaga
     8401 agcaaacatt ctcccttatt ttgaagaaat gcatcaacta gaaacgtcca tgaccaacca
     8461 acttgctttt gaatacattt ttattgatga tggttctaag gataacacct tgggtatttt
     8521 gcgtgagcta gctgctcgct ttccaaatgt gcattatctg tctttttcac gccattttgg
     8581 caaggaagct ggactcttag ctggactaaa agaagccaaa ggcaattgca ttactgtgat
     8641 ggatgtggat ctgcaagatc cacccgaact tttgccaatt atgtacgcta aactaaaaga
     8701 gggttatgac atcgttggca ccaggcgcca aaatagacaa ggagaaccac taattcgttc
     8761 aatgtgttcg aatctatttt atggactgat caaacactta tcagatactg aaatggtcaa
     8821 tggagttcgg gattatcggt taatgaccag acaagtcgtt gatagcattc ttgagttggg
     8881 agaggttaat cgtttttcaa aagggatctt ttcgtgggtt ggttatcgta tcacctatct
     8941 tagttttgaa aaccaaaaac gcaaacatgg aaaaagtcgt tggcattttt gggagttgct
     9001 ccgatattct ttagatggct ttatcaactt ttcagaaatg ccgttaacca ttgctacctg
     9061 gacagggact ttcagctttt tgatttctat tttcgccatc ttatttatca ttatcagaaa
     9121 gatactcttt gatgatcctg tctctggttg ggccagcact gtctctatta ttttattcat
     9181 gggaggcatt caactctttt gcatgggtat tattggaaaa tatatttcaa aaatattcct
     9241 tgaaactaaa aaaaggcctc tctatattat caaagaaaaa cattaaatta aaacattttt
     9301 ctcatcaatc gtttcctact ccttctgtgc gcgaagcttt ctttttaagc ccgttatacc
     9361 aaatgataat gacctttcca aaatagtatc accttcctta acactttaag tagaaaagga
     9421 actattaggt tgagcctttt tgcaaatcct agcgactaat gttataatcg tcatactatt
     9481 gaaaaaggag ttctttatgg agtggattaa attaatcggt attgtgatta tcgtattagg
     9541 gtttatttta aaatttgatg ctatcgctac cgtagttgtt gcaggacttg ttaccgcgct
     9601 ggtttcagga atttcgttta tcgattttct agatatttta ggaaaagaat ttaccaatca
     9661 acgtttgcta accattttct tcatcacttt accgcttatc ggcttgtcag aaacttatgg
     9721 gctgaaacac cgagctactc agttgattca acgtgttcaa tcccttaccg tcggccgttt
     9781 attgactctc taccttatta ttcgagaatt agcaggtctc ttttcgattc gactaggggg
     9841 acatccacag ttcgttcgcc ccttgattca acctatggga gaagctgctg ctaaagccaa
     9901 cattggagaa gagttgaccg atgcagaaaa agatgacatc aaagctatgg cagctgccaa
     9961 tgaaaacttt ggcaatttct ttgcccaaaa tacctttgtt ggcgcaggag gtgtcctact
    10021 tatcgctgga actttagagc agcttggcta tgatggtaac caagctaaaa ttgcattttc
    10081 ttctatttta attgctatta tctctattat tatagttgct atttacaatt acctctttga
    10141 gaaaaaaatg gaacgtcaac atcagaaagg agataactaa tggctaacat tgcaaacctt
    10201 gttttagaaa cgatatatat ccttatcggc ttgcaactgt ttcacacagc ctactgcacc
    10261 tttaaagata aaaccaaccc tgtctatttt gggacagctc tgttctgggg attgctgggt
    10321 gttaccttcg ttggcggtgc ctttttgcct aataaagtta tcggttttat cgtgattgtc
    10381 ctagctcttt taaccttgtt taagcaagtt cgtattggaa cattaccagc ctttaatgaa
    10441 caaaaagctg aagaaagtgc tcaccgcatt ggtaactgga ttttcttacc agttatgtta
    10501 atggctatga tttctctact gcttgccttg atccttcctg atttcagtaa aagtgccatt
    10561 gggattgctg gtatcttagc cactattgct attttaataa tcactaagca aaaaccatct
    10621 gctcttttag ctgaaaataa tcggatgaac caacaagtgt ccacaagcgg tattttgcca
    10681 cagctcttag gagctttagg agctattttt gcagctgctg gtgttggtga tgtgattgct
    10741 tctctcattc gggagattgt acctgctgat agccgcttct ttggtgttct cgcttatgtg
    10801 cttggcatgg ttatttttac tatgattatg ggaaacgctt ttgctgcctt taccgttatt
    10861 acaaccggta tcggtgttcc ttttgttttt gctttaggag ctgaccctat tattgccggt
    10921 gctttagcta tgactgctgg tttttgtggt accttattaa caccaatggc agcaaacttt
    10981 aatgccttgc ctgttgcttt gatggagatc aaagatcgaa atgcagttat caaaaagcaa
    11041 gctcctatcg cccttgtttt aattattagt cacattgcct taatgtattt acttgcttat
    11101 tattaacttt tttgctaagt tagtagcgtc agttattcat tgaaaggaca ttattatgaa
    11161 aattcttgta acaggctttg acccctttga cggcgaagct attaatcctg cccttgaagc
    11221 tatcaagaaa ttgccagcaa ccattcatgg agcagaaatc aaatgtattg aagttccaac
    11281 ggtttttcaa aaatctgccg atgtgctcca gcagcatatc gaaagctttc aacctgatgc
    11341 agtcctttgt attgggcaag ctggtggccg gactggacta acgccagaac gcgttgccat
    11401 taatcaagac gatgctcgca ttcctgataa cgaagggaat cagcctattg atacacctat
    11461 tcgtgcagat ggtaaagcag cttatttttc aaccttgcca atcaaagcga tggttgctgc
    11521 cattcatcag gctgggcttc ctgcttctgt ttctaataca gctggtacct ttgtttgcaa
    11581 tcatttgatg tatcaagccc tttacttagt ggataaatat tgtccaaatg ccaaagctgg
    11641 gtttatgcat attcccttta tgatggaaca ggttgttgat aaacctaata cagctgccat
    11701 gaacctcgat gatattacaa gaggaattga ggctgctatt tttgccattg tcgatttcaa
    11761 agatcgttcc gatttaaaac gtgtaggggg cgctactcac tgactgtgac gctactaaac
    11821 ctattttaaa aaaacagaga tatgaactaa ctctgttttt ttgtgctaaa aatgaaagac
    11881 ctagggaaac ttttcatcgg tctttctcaa ttgtcatctt aatctaatac tacttctaac
    11941 atcagcgggt atagtttgcc agtaattaag aaacgttgtt gatctaaatg agcaatccca
    12001 ttcaaaacat taaggtcagg gtaatgggac ttatcaagat ttaaggcttt taacaaagga
    12061 ctaatatcat aggtggctac cacctttcca gaatcaggtt ggagtttgac aatagtattg
    12121 gtttgccaaa tattggcata gagataacca tctacatact ctaattcgtt aagcattgag
    12181 atggggacac tttctatagc aactagtacg gtatccaata aagcaaaatc tttggggtct
    12241 cgcttctgca aaaaagcatt tccactagtc atccaaagac actggttttc cttatcatag
    12301 gctaatcccc agccatctcc ttcaaatgga taggccccta aacaattgca ggtagcctta
    12361 tcaaacttgt aggctacacc ctctttatag gttagtaacc aaaaataatc ctcaacaact
    12421 gttagccctt ctgcaaaaac tgtatctgga aaagctattt tttctgaaaa gatctcttga
    12481 gtaagatcat acacccctac ttttgagaaa ccataacgac cggcactaag taatatatga
    12541 ttgttgttga gttgttctaa tccctgcgtg tataagttgc tatcataaga ataagttcgt
    12601 aacaaacgaa cttgtccttt agttatcata atactcattc accttacctt gcattgtagt
    12661 gtttcatttg ttttttaata tctctttcct gatcacggcg tttaatggtt tcgcgtttgt
    12721 catactcgtg tttcccttta gctagaccaa tcagaacttt agcaaagcca tccttgaggt
    12781 aaactttgag aggaaccaaa gtcatgccag atcctttaag ttcatttgct agatgcgtaa
    12841 tctcacgttt tttgagcaac agtttgcggg tccgttcagg atcagcgttc caaatattac
    12901 cttgttcaaa aggtgcaata tgcacgttga ccaaccacgc ttccccattc ttaatttggg
    12961 caaagccatc tttcagttga atacgggctg cacgaacact tttaatctca gttcccgtta
    13021 agacaatccc tgcttcaacg gtttcaacaa tgtgataatc atgtctagcc tttttatttt
    13081 gcgccagtat atgaccttca ccttttgcca tagttaactc cttttacgac tcttcttttt
    13141 tgcttgttct ttgtaaaatg gttttttaga ccctttttta gttttaccct tggtttttgc
    13201 cttggctact tctttaggtt cttttttctt tatcccttta gatgattttg aggatttacg
    13261 gcgatctcgt cgactagctt tatccgacat cttgattttt tcaaccacat caaagtcgct
    13321 aggtaaatac tcaaagtcaa tgtcaccagt ttccttgtca gccttaacca atttcacacg
    13381 aataggttga ccaactttaa agactttacc tgatttttca ccttgaaggc ttagcgttct
    13441 ctcgttaaag tgatagtatt ccggtaagct agtgatatga accaaacctt caatcgtatt
    13501 tggcaattct acaaaaaaac caaatttaac gacactggcc acgataccgt caaattcttc
    13561 gccgacatac tctgccatat actcagcttt tttcatggct tcaaccacgc gttcagcatc
    13621 aatagcacga cgctctaact gtgaagaaga tgttgccagc tctggaatga tctgagcaaa
    13681 atggtcgcgc ttttcttgac taggttgatt gtactcacga accatgcggt gaaccaacaa
    13741 gtcagggtaa cgacgaatcg gactggtaaa gtgagtgtaa tattctgcgg ccaaaccgta
    13801 atgcccatga ttatgttcag aatatcttgc ttgttgcatg gaacgtagaa gcatcatgtt
    13861 gagcacttct gctcctggtt gaccttcgac ttttgccata aaggcttgca aggcttcctg
    13921 agaaatcttg ttggctgttc cttgaatgtg aataccaaag gtgctagcgt agtcaataaa
    13981 ttgttgaagt ttttcaacct ttggttcttc gtgaatacgg tagataaacg gtaacttagc
    14041 ttttgcaaaa tgttctgcca cacactcatt ggctgctagc ataaaggatt caatcatccg
    14101 ctctgcaatt cccctttgac gaagcaccac atcaaccggc attcctttgt cattaacaat
    14161 aactttagct tcttgcgtat caaaattcaa ggcgcctcgt tttgaccgca tcgtttccaa
    14221 aatcctatgc aaagccacca tgagggtcac atcatcagca atgctagcga attcttggag
    14281 cgcttcttca tctcctgcaa tcatgtcatt gaccgtacta tacgtcattc ggtaagttgt
    14341 cttaatcact gattgacaga tttgataatt gaccacatac ccctgcgggt taatttccat
    14401 taaggctgac tgggtcaaac gatcaacgtt agggttcaaa gagcagatgc catttgacaa
    14461 acgctctggt aacatcggaa ccacacgatc ggtgacatag acagaagtgc cacgggcaat
    14521 agcttcctta tcaagggaag atccttctgt cacataatat gagacatccg caatatgtac
    14581 cccaagttca tagttaccgt tatccaatag cttaatatgg atagcatcat ctaagtcttt
    14641 ggcgtccgca ccatcaatag taatggtggt ttcttgacgc aaatccacac ggcctattaa
    14701 atctttagca gttggtgctt ctgaaatagc attggcctca gctaaaactt ctgctgggaa
    14761 ctctgacacg atatccatcg attcaaggac ttctaggaca tcaattccaa cgtctccttg
    14821 atggcctacg atatcacgca cactggctac aaaataatcg tgcccgcgtg tcgggtattt
    14881 atcaatatca accttaatga tttcagtgcc tttaagcaca acaggctctt ttttgatata
    14941 gattttttgt tgaatctttt gatttttgga cctgatataa ccagcatatt ttggtttgtc
    15001 atcatctaaa ataaaggttc caacagctgt ttttaaagaa cggtcaacaa tagcaacgat
    15061 cttagcttca gctgctgtgc ctttgagacg gtctgctggc cttttaacca ctacttcgac
    15121 agtatcacca tcaatagcat acccaacatc attacgaccg ataaacatat cgtcttcatt
    15181 ctcatccaca tgtaagaaac caaagcctgc tttattggca cgaaaaacac cctgaacagt
    15241 aggttctttt ttcttttcac gctcttttct aagagccagg ctgccatcat ctgaaaaccg
    15301 taacaggctc tgactttcca ttttggaaat ggttttaata aggctgggaa aatgcttagc
    15361 tccagccatc tccaaggccg cagccaagtc gttcacattg gacttgccat gctccttgag
    15421 gtaagtcata attctttctt tcatcatacc taatttctat ttctgttttg tttgttggag
    15481 gatatcaatc cgtttcgctg ataaaaaaat gagctagact aactctgtca agctcactcc
    15541 ttatttactt gataaaatgg caagcacaag tgcaatcgct agccaaaaaa atactagaac
    15601 tgctgtgaat cgttgcatga atgcttcaaa gccacgagct tttgtacgtt caaacaaggc
    15661 ttctgaaccg ctgctatcaa aaacattgct acttgggttt ttttgaggtt gcataaaaat
    15721 agcaatcaca agcaataccg acagtactag caatatcgta agtagtaagt tgtacatgtt
    15781 atttcctcca ctattgtcat gaccattcta acacaattct tatcaagttt caacatgcag
    15841 ggtcactttt ctctccttgg ggcagtattt gggaactttt attttttccg gatgactgct
    15901 tttattttta ctggttagat aattgttact gccacactca ctacattcta aattaatttt
    15961 tactctcaca caatatcctt aactttaata tattttcgaa atataatcaa actccatatt
    16021 aaattaacaa acacaatcaa actagtcaca taaaaaacag aacggtagcc tagcactaca
    16081 gccacatttg atccgataaa agggccaatg acttgaccaa ggttactaaa catttgatta
    16141 taggcaaata ctctagaaat accttctttt ggtgttaact tagttagtaa agagttaata
    16201 cttggcatca aagcgcccac accaaaccca taagcaaagc gtagtactcc taattgaaaa
    16261 ctggtctgcg ctaaggcatt gctaaaatac atgataaagc tataacacaa agcggctaac
    16321 aataaccgat gattgccaaa tcgatctcct aattttccta agtaagaact gctaaataag
    16381 ctagaaaacc ccatggccga aactaccagg cctgaagtaa acattaagtt gtgagtttga
    16441 ccaagatgac gaatatacaa tgataaaatg ggggccactg attgagctga aatttgaata
    16501 atcatactag tcacaaataa accaagcata atctggggac tttttacctg tttttaaatg
    16561 acttttgtag gtatcatctc aaagcgccta accggcttaa actcttcttt aacataaaca
    16621 gctgtcatta aagaacataa aaataatatc acaccaacta ataagaaaac ttgtcgaatt
    16681 cctaatagct ctgccaaaat tccccctagt aaaggaccaa ttaaagcgcc tgctgtcact
    16741 cctgttgcta atgtgcctaa agcataacct gactcctctt ttggggcttg gctggcaatc
    16801 aaggcagtgg catttggcac atagcccgca aagacacctg ttaataatct caaaatgagt
    16861 aaccaaaaca cattaggaat aatagctaaa ccacccattg taaatgtcat gacaaaactt
    16921 gctcgcagca tcataggttt tcggccataa cgatcagcca acttccccca aacaggagca
    16981 aataaagctg aagcaagtgc cgtcacagca accgccagac cagcatagta ttctactaat
    17041 tctttcggag tacctaaatt ttccacatat agagccataa aaggcataac aagtgaaaag
    17101 cttgccccag taaagaagtt accaagccaa gccactttta gattttgtcg ccagttcact
    17161 gtcgttaccg ctttatcact aatagatcat catcttcttt ccatcgggcc tttgattatt
    17221 ataacacatt tctgccaaaa acagtgtccc ataatagtgc taagtctcat ctgatagcct
    17281 tcatcgttta cgtaaactac ccagtaaacc tctgaccagt tctcgcccaa ttgttctaac
    17341 cgtcgtacta ataaacgcat ctgttgcttt ttcgacgact gttttgcggg gacgcccaac
    17401 agaacggcga ctagtctttt cagcagcagc ttgttccttg atggcttctt tagcttcttt
    17461 ttcgcgttgt gctgtcgcta tcgcttcttg ggccagtcgg ttgtcttcta aaaccttagc
    17521 agctaatttt tcataagcag agtcacgatc aatggactga ctatatttac tggcaaaagg
    17581 tgaggactgc acaagctgct gactttctat atctgacaaa acagcaaagc tactcttagg
    17641 cggcatgata taggcacgtt caacgatgga tggttgtccc ttatcattca aaacagaaat
    17701 caaagcttcg ccgacttcta gttctgtaat cacccttgcc acatctaagt caggattttg
    17761 tcggaaagta tccgcagcga cacgaaccgc cttttgttct ttcggtgtat aagcgcgcaa
    17821 ggcatgctga atacgatttc caagctgagc caaaaccgtt tctggcaaat caagtgggtt
    17881 ttgagtcaca aagaaaatcc caactccctt tgaacgaata agtcgcacaa tctgttcaac
    17941 tttttccaag aaaactttag gggcatcttt gaagagcaag tgagcttcat caaaaaagaa
    18001 aaccatctta ggtttgtcca aatccccaac ttctggcaat aattcgtaca gctctgataa
    18061 taaccaaagt aagaaggtcg tgtaaagtgt gggtgattgg aaaagcttag tcgctgacaa
    18121 aatgttaatg gctccataac ccgaagcaac atctaattgc atcaaatcag ccacctctaa
    18181 agcaggctct ccaaaaaatt ggtgggcccc ttcttgttct aatgtcaaga gacttctttg
    18241 gatagccccg atagattgct tggcgatatt accgtaatgg ctggagtagt ctgaggcatg
    18301 gtcaccaact tcttttaaga tagcctgcaa gtctttcaaa tcaatcaaaa gccaaccttt
    18361 ctcgtcggcg attttaaaga cgatgtttaa gacacctgtc tgggtatcat taagattcaa
    18421 taaacgcgac aacatcatag gtcccatttc agaaatcgtt gtccgtaagg gttgaccgtt
    18481 ttgaccaaaa acatcccaca ttcttacagg aaaggcctga ggctggtaat cacttacccc
    18541 gattgtggct aaacgcgcag ccaatttatc agtcacctct cctgctttag taagattaga
    18601 caggtctcct ttgatatctg ccaaaaagac tggcacacca gctaaactta attgctctgc
    18661 tagcactttt aaggtcacag ttttacctgt tccagtcgct ccagcaatca aaccgtgacg
    18721 atttagcatc cctaattgta aggctatcgg ttgattgcct tttgcaattg tcattgtagt
    18781 cataggcttc ctcttttttg atgatcatac ttgtcgatat gtgtttaccc ataagttaaa
    18841 gtgtttagaa gggtctcact cttaatacta ttataacata taacccttga tgcgctaatt
    18901 taaaagggac cagctagttt tagacagaaa tagcttgatt ttgacagcta tgatacgaat
    18961 ctctcagctt agctacctcg gattggctaa tgacaacaac gcagattgta cttgctttat
    19021 taaggctgca atatccccac tattatcaat cactagactg gcatagggct ttttttcctc
    19081 tattggtaac tgactggcta ttcgctgcct tgcttttcct ttgtctaaac gatttctagc
    19141 cattaagcgc tgcaattgtg tctgagcatc tacataaacc agccaaatcg catcaaacca
    19201 gtcttggtag cccaattcca ttaaaagggg aatatccatg aaaaaaatgg cttggctctg
    19261 tgctaagtgg tcacgcttag ccgccaattc ttccttaatg atttgatttt gaatggctga
    19321 ggatgttgcc atattatctg ggtttgaaaa aagcatttct gacagctttg tgcgatctaa
    19381 ctcaccatct gcttttaaaa tctgattccc aaaagcttct ctaagagcct cgtaaagacg
    19441 accgcctttt tcttgcaagt catggaccac ttgatccgca tcaatcacct gataaccagc
    19501 ttttctaatg accttaacca cagtcgattt tcctgatgca atgcctcctg tgatgccaat
    19561 aatcatggtc tttttttctg gcattttgga caaatgtgcg tcccccttcc tccaactttt
    19621 aatttgacaa tagcctgtcc acaacgagga cacggtttcc cagtttgacc ataaacctgc
    19681 aaatagtcct gcatggtgcc atcagctccc aaagcattac ggtaagtacg gacggtcgaa
    19741 ccgccctttt caattcctaa agctaggatt cgtatggttt catcatgcag ccgttttatc
    19801 tccgccttat tcaacctaga agacgcagtt tctggatgaa ccttagcagc ccaaagtacc
    19861 tcgtccacat aaatattccc taaaccagca accaaggtct ggtctaacag atgtggtttt
    19921 atgggttttt gggaggatag cagagcagcc tcaaaggttt ttaacttaaa ggtctctttc
    19981 tttggttcag ggcctagttt tcgtttggca aaaaacgctg ataattgaga ttttgctatc
    20041 aaatcaaagg ttccaaattt acgcacatct tggtaaacca aggtcgaacc atttttcaat
    20101 tcaaaaaaga catgaaagtg tttgttatca gggacctcat ctggaaaaag caaatatttg
    20161 ccttccatcc gcaaatgtga taccaacact aattgcccca aatcaattaa taaatatttt
    20221 ccccgacggc caactgactg aatgatttga ccaggtaggg tcagggcaaa cgtctctaag
    20281 tcggttttaa ccatttttgg tactttaagc gtaacagcca caatttcttg gccaagcact
    20341 aaagtctcaa gccctcgtcg gaccgtttca acttctggta attctggcat aatccgttat
    20401 ttaaaggaca gctagaccct ttgctgctac aaagaataaa ctcagtataa aaaagtcact
    20461 caatagactc aggtctaggc tactgactgc gtttcaaaaa acacagctgt ccttctcctt
    20521 tcttatttct tattttacga acacatctga tagaaagcaa ctcataaatc tgcctcagac
    20581 cagtcctcat cccccttacc tcccagcact atttgacggt aatgttcctt ataactataa
    20641 tacaatgagt cctctccaag atacttaaaa atctgcaagg cctgccgcat gtcctcttct
    20701 ccagacattt cttcctgctt taatttataa tacccatgca catataagaa aacagtcttt
    20761 tcataaaaat tgagctcgtc tcgcagcaaa agatcaattt tgttaatcaa atagcgacta
    20821 tgttcaaagc aagaatggtc aatactgata atgagacaat taatggataa ctgagtcact
    20881 aacattttat tggttttatt ttgttccgaa taagcaaaag atgctaccat ttccttagtc
    20941 aataaaaata gggtattgta attcatgaag cgcgagcaat tccccagaag aatgatttca
    21001 taatatcccc actgttccac tttaaataaa taatctgtga ggcgagtcaa ttcttcttga
    21061 ctaggtgcaa ttgaggagtc aatagaaaaa agaatagctt taatcatcgt tcgttcataa
    21121 tctttatggg cataatcctt caacagtttg gtcaatttaa ccacgttttt ctcagcataa
    21181 cattttctcg cctgacttaa gagcgtgaaa aaatgcgtat gcgtcttgga atgagccgaa
    21241 acaaattcgt caatcgtaat attcagttta tccaaaagat taagcaagcg actgcaggtg
    21301 atttctgatt cccctcgttc aaatcttgaa atttgtgact tggacaggta ttcatctgct
    21361 aaaaaagaaa tgctaacctg cttaccctta cgtaatcgcc ttaaggtttt tccgagttct
    21421 ttttccattt ttcccacttt cacaacaaaa aattaaaatc tgctaaatcc attatataat
    21481 acaagtacaa aatagaaaat cagaaaggag acagtcatga aaaaagttaa taaagctttg
    21541 ctttttacat tgatcatgga tatcctgatt atcgttggtg gataaccttt cgcttaaaaa
    21601 caatccactt ttgggaaacc cttatattta gtttgtaaca ggaaaactaa tagatattaa
    21661 aaagagtgtt agcgacctag cggcagcttt actcttaagt gcgtttgctg ccattgacag
    21721 caaggtcaaa gctgatgaaa cagcaggggc ataccgttat gatgtgtcta aacaaaacac
    21781 ttactatcaa actatcagct ccattaacca gcaacgacct ggtgccactc tcgacgaatg
    21841 aggtgattat tatggaaccc ttacttcatt tacttcccaa agtcgtacca gtacttatac
    21901 acttggtaga caaagccaac gtatcatggt taacagtgat attacagtta cctatactct
    21961 taaagacaat ttggtagccc ttcataagga atataaaacc tttgtcaaaa aaatattaca
    22021 gataaaccga tcaccagtga ctacgacaag gctaaggtta tttataacca tttaatcaac
    22081 gaatacactt acgccacaaa ataacttagc accaatcgca aaaccgtaag cagcatcaac
    22141 aatcatactc ctaaagctct ttatcgtggc aagtgtggtg cttgtcaagc ttatgccgtc
    22201 atgtttaaag atatggcaga agctgcaggc ttatcaactt ggtatgtgat aaaaaaggat
    22261 tgaatgagca ccatacttga aacatcgtta ccattcatgg tgttaattcc tacgttgatg
    22321 ctacctggaa tgattcagca tacaccacaa ggtatttcct tgctggaaaa gacgtgagga
    22381 atactgagta ccgtttagat agccagtaca accacttggc taatactatt ttaaatacca
    22441 gctaccaaaa aggctaaact aacaacaact acatttttca actagaaaga cctaaacaat
    22501 gaccaaaaaa cagttttatc ttatcttagc tattagcata ctgtgtttta ttcttgccgt
    22561 cagccctaaa ccaccaattc ctaaacacac tattgaaagg aaaaggactg cgatgaccat
    22621 aggccactca aaacaaaacc ctcacaaaaa agaagccctt aaacacaaaa aaaataaaaa
    22681 taaggctcta tttcttccaa gtggggacag cgctatcaaa tctgttatta gcatgacagt
    22741 tagactcctg ttagatgacc aatcttagct ctcctaaaat cagtggacac taccatttcg
    22801 gtagtgttct tttttaagct aatctcatca ccacgctttc ttcaagctaa aaaactgatc
    22861 atgaaagagc aaccccatca ccctaacagc ccactttgcc cgcactttct taaaaagtta
    22921 ggagaaaacg atataccaat tattttctga tcccagctcc ctatcattaa caagatgtgt
    22981 agcacggacc tacatacagg taagctagag tcattctcgc tatttcggac aaaaagaggc
    23041 cggtagttcc ctgacctctt ctttatttat cttgtaacac tcctcttctt agcgagactt
    23101 actagcacat aattcttaag cactcttatc atttagacta ggaataacac catctgaatg
    23161 aagtaaatga tgcgccagat ctgccagagt atcatctcca taagtaatcc ctgtttggtg
    23221 ctgtttctga tgaaaatcag atgactcctt aactttagca gtcacctgct ggtcacttgg
    23281 atgaaaatta atttctctct tggcttcagc cgcaactggc tcgaggaaca acttattgac
    23341 attacggtct tcctccaaat gattaaggat agcagcatca tcttctaacg aaagaacctt
    23401 cttgggtaac aaaaaggggt tatttagcct tggctcagta acgacttcct tggctactcg
    23461 tgatagttta ttattgtctt cctcaggtat caaacggtta ggcaatcgat gcaattcttc
    23521 cggctgtgag gcgctcacag ctacttcaga agaaacctcc aagccctttc ctgtttgcct
    23581 gtctaaagca accggcaaat tattaactaa ctttttaacc tgtcctcagc gctcatttca
    23641 acactaggaa gagctgtcgc tggttctagc cagctaccaa cttctgctgg tcttacctta
    23701 acatcgctgg caggcttctc cttctcatct tcattaggtc taaaaggctc aaaccgccct
    23761 gttgattttt catctatgac ctttatattg tcttttacta catgagacag tggttcgaca
    23821 tttaccatag cttcatgctc gttaaggctc tggtctcgtt tgaatttacc aggaatacca
    23881 ggagtcggtg ttccgacaga ttgatcggct tcagtaggct cttttacgga gccttttttc
    23941 cataaaactg ctggaggaat aatatccgta gagccgtccc cataagtaat cgtggcctgc
    24001 ctcccctttg gattaataga tactttaata ttgtctggga aagccccact acctcctgtg
    24061 gttaaaaggg cacccgcatt tttaatgaga gttgtcattc gttcaatacc agcaacgcca
    24121 atatggttca agtcatctac tggtaactga tcaacagatt tcatggtgta tttccaagct
    24181 tgcggttgaa caagaaaaac agttccttta tacattgtgt tatgcatatc atcaatgact
    24241 ttgaaatacc ttgtatattt ggtttgtaca ttagggttag caacgggaac agtgctttca
    24301 ggaactcctg acatcgtaaa aatccctcta gcagaagttt gaaagccctc ataaacatga
    24361 cccataccag gaacaatctg agcggtcaaa gacttatcag aaccatccaa agcaatgcga
    24421 atatctttca tcagagacat ctgattggta taggtaaacc taaccgtgtt agcctcacct
    24481 cgatacaaaa ccacttggga gggctgatcc gaagaaaact gagaggttgt ttcttcctta
    24541 tagccaactc caaaacgtct atcagacgaa tggactgaat ctgccgcaac tgtaccactc
    24601 aaactgacta gtaaagtagc cgcacaacca actaataaac tgctcgtttt aactttaaca
    24661 tctcttaaca tcatcatgtt cctttcatta ttttttattt aggaattcgt catactatat
    24721 tttctcatat tagtctaaca aaaaactcgt aattctatat aaatcatata aaatgacgag
    24781 ttttggcaaa gatgagtata gcaggataga agacagatt
//
LOCUS       DAUCWU010000015        24051 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUCWU010000015 DAUCWU010000000
VERSION     DAUCWU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24051
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.015"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            47..322
                     /locus_tag="VGY24_000613"
     CDS             47..322
                     /locus_tag="VGY24_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ4283617.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGAPDRIVVMNTGIFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMENWV"
     gene            319..1641
                     /locus_tag="VGY24_000614"
     CDS             319..1641
                     /locus_tag="VGY24_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ4283618.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEKTWPTEIEKWHFDFTYSKVLGDERKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENIVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            1642..1812
                     /locus_tag="VGY24_000615"
     CDS             1642..1812
                     /locus_tag="VGY24_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283619.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKPDMLKFWNSCEAT
                     VKEKYTREREND"
     gene            1805..2077
                     /locus_tag="VGY24_000616"
     CDS             1805..2077
                     /locus_tag="VGY24_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283620.1"
                     /translation="MTEEQMIDCLLYELVKKDKAIKKKNIIITALTVMLLIVVSGLGI
                     SLKSYYEPQIYGLRAQLSRTQKQLKRASDDRARQTKRIAELTKNGG"
     gene            2210..2626
                     /locus_tag="VGY24_000617"
     CDS             2210..2626
                     /locus_tag="VGY24_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4283621.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTAVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            2716..3168
                     /locus_tag="VGY24_000618"
     CDS             2716..3168
                     /locus_tag="VGY24_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ4283622.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            3158..4435
                     /locus_tag="VGY24_000619"
     CDS             3158..4435
                     /locus_tag="VGY24_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ4283623.1"
                     /translation="MTIKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSALLHV
                     KIDDANKKLYVLEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTIMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            4450..5982
                     /locus_tag="VGY24_000620"
     CDS             4450..5982
                     /locus_tag="VGY24_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ4283624.1"
                     /translation="MEEKQFLAGTRFNENANRQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDEKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQITKVYTSDTIYTYEDYN
                     LETKGMRLKDYEEHFFKGVTVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNAYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANVWAVKGNEATTYGLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGGANGQEEPTAE
                     KP"
     gene            5948..7390
                     /locus_tag="VGY24_000621"
     CDS             5948..7390
                     /locus_tag="VGY24_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ4283625.1"
                     /translation="MVKKNQQQKSLNYWQKRQEDILSYLDRADLDVFSELQKLYSEQA
                     FELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLKRL
                     NEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKAASYAYKKAMGGRSGTIN
                     GPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKRLKQGFVRGEHPRAMARDLAKRFN
                     VANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRTIAKENKRK
                     LLSELKPGINVAPYHFNCRTTIIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNEHK
                     PQLSLLNEYVKNGTPYKVDGHSVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPEHI
                     KTPDYLVDGAPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQLE
                     HIYMIDRRGLDISIIKNKDEVLAVLEKEGG"
     gene            7418..7606
                     /locus_tag="VGY24_000622"
     CDS             7418..7606
                     /locus_tag="VGY24_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283626.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKEG"
     gene            7611..7877
                     /locus_tag="VGY24_000623"
     CDS             7611..7877
                     /locus_tag="VGY24_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283627.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEAIKNQNKQIMELKSIVQ
                     RNALATNEELATVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            8039..8608
                     /locus_tag="VGY24_000624"
     CDS             8039..8608
                     /locus_tag="VGY24_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ4283628.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEHPKHEYERTFTRADISKMMAAE
                     RTKWESEQSAAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLATTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            8621..9508
                     /locus_tag="VGY24_000625"
     CDS             8621..9508
                     /locus_tag="VGY24_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ4283629.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            9520..9876
                     /locus_tag="VGY24_000626"
     CDS             9520..9876
                     /locus_tag="VGY24_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ4283630.1"
                     /translation="MGDKQLIDDIKLFIGISKGDGAQDEIITLAIYESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            9887..10165
                     /locus_tag="VGY24_000627"
     CDS             9887..10165
                     /locus_tag="VGY24_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283631.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKSRLIKGLAHHKNSTVIYV"
     gene            10162..10506
                     /locus_tag="VGY24_000628"
     CDS             10162..10506
                     /locus_tag="VGY24_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ4283632.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARVERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQSFLDPALRKEWP
                     VLMANIKKMFKR"
     gene            10510..10869
                     /locus_tag="VGY24_000629"
     CDS             10510..10869
                     /locus_tag="VGY24_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283633.1"
                     /translation="MDYSLETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            10881..11480
                     /locus_tag="VGY24_000630"
     CDS             10881..11480
                     /locus_tag="VGY24_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ4283634.1"
                     /translation="MANTKKGTTIEITTGKPIVGKKIFYFIQSVDAPKKSQALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQIPESTGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            11534..11989
                     /locus_tag="VGY24_000631"
     CDS             11534..11989
                     /locus_tag="VGY24_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283635.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAEAVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDMDATQIQIIEDMIGRMSNAVS"
     gene            12064..12297
                     /locus_tag="VGY24_000632"
     CDS             12064..12297
                     /locus_tag="VGY24_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283636.1"
                     /translation="MEGLEYKQLAERESLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            12312..16694
                     /locus_tag="VGY24_000633"
     CDS             12312..16694
                     /locus_tag="VGY24_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ4283637.1"
                     /translation="MPGTFDGSIFADVGANTKDYEQAMARIVSTTQNAFRKAQDIAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVIDKIRGNLTGFINQTKMQLQNLVDIGKIRSAFTKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGQQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVAQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFNQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLGDFAHTGELSGQTFDKLKAKIASLAPKVIALWA
                     VMNPASAIATIMPLLSLFGKVGLALGSLGTSVGAFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVVGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IDQLLNTAVTKGPGIIQGLVKGITSKIPALIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATNLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIDSFGNSLQANLPSIISNGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIAQSAVQIIQFLASGLTQALPQIIAAG
                     IQLVAQLAVALIKGLPQIISAGIQLIMGLGKAMLGAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATVMAMNVGMQTTAMANQANTDTTSMLNSISQNTELANINATS
                     NATQMANNVNAQTGTMSLQGLNDSMSLASGISANMSVANINATTQAREMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGAASTGATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSVTSKMSSAATSATSSVNRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFD"
     gene            16706..17548
                     /locus_tag="VGY24_000634"
     CDS             16706..17548
                     /locus_tag="VGY24_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4283638.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTVSNVERLLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFALRTAELP
                     FGETKPKDITLSGGSFAYAGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            17558..19537
                     /locus_tag="VGY24_000635"
     CDS             17558..19537
                     /locus_tag="VGY24_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4283639.1"
                     /translation="MITFLDHRDIEYGVISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLTRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINQRTDKELEEFRATL
                     KNLALPEEAIKKITEAIKVDDIPSIKQSFDDLKNRVSETSEESRLTAEILGNNGKTRY
                     NKNLLVGDPNRVKKIDEDYIEVEANDGGFKRGETYTISFSQTCELLQKVAITLTQANN
                     KGVKLVLTPTKAKMESQTFDLTKDKEVISVYPFSYTVLVTSDWYKSKQIDLNASEVKE
                     LALEMDYKEIADAKGATITGAWSDSPQIILDGGKK"
     gene            19534..20547
                     /gene="hylP"
                     /locus_tag="VGY24_000636"
     CDS             19534..20547
                     /gene="hylP"
                     /locus_tag="VGY24_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192934711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase HylP"
                     /protein_id="HEQ4283640.1"
                     /translation="MSENIPLRVQFKRMTASEWARSDVILLESEIGFETDTGFARAGD
                     GHNRFSDLGYISPLDYNLLTNKPNIDGLATKVETAQKLQQKADKETVYTKAESKQELD
                     KKLNLKGGVMTGQLKFKPAATVAYSSSTGGAVNIDLSSSRGAGVVVYSNNDTSDGPLM
                     SLRTGKETFNQSALFVDYSGKTNAVNIAMRQPSTPNFSSALNITSGNENGSAMQLRGS
                     EKALGTLKITHENPSIGADYDKNAAALSIDIVKKTNGAGTAAQGIYINSTSGTTGKLL
                     RIRNLSDDKFYVKSDGGFYAKETSQIDGNLKLKDPTANDHAATKAYVDKAISELKKLI
                     LKK"
     gene            20562..22469
                     /locus_tag="VGY24_000637"
     CDS             20562..22469
                     /locus_tag="VGY24_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ4283641.1"
                     /translation="MSRDPTLILDESNLVIGKDERVHYTFTAEDDNPKVRLASKCLGT
                     AHFNQLMIERGDKATGYVAPVVVEGSGESTGVFKSLEEMLSGLQSINLELTDTKNSNL
                     WSKIKLTTNGMLREYHRDNITTEIIEKADGIATRISEDSDKKLALINDTIDGIRREYQ
                     DADRELSASYQAGIEGLKATMASDKLGLQAEIKMSARGLSQKYDDEIRKLSAKITTTS
                     SGTTEAYENKLEGLRAEFTRSNQGMRIELESQISGLRAVQQSTASQISQEIRDRTGAV
                     SRVQQDLDSYQRRLQDAEDNYSSLTHTVRGLQSDVVSPTGKIQSRLTQLAGQIEQRVT
                     RDGVMSIISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNT
                     TVNGTFTTKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAIT
                     DLLEGKVIKARNGAMLINLNNSNIDFNSNATINFNSGNNALVRRDGTHTAFVHFGNAT
                     PKNYAGSALYAAIGITSSGDGINSASSGRFAGLRSFRYATGYNHTAAVDQTEIYGDSV
                     LVVDDFNINRGFKFRPDKMTKMIDMNDLYAAVVALGRCWKHLANVGWNTVHGNFVSAV
                     NGELNNYITKI"
     gene            22481..22909
                     /locus_tag="VGY24_000638"
     CDS             22481..22909
                     /locus_tag="VGY24_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ4283642.1"
                     /translation="MQLTIKNKDLNTLYGVLDKIKITNMRANRGRAKLLAKVEGKLSE
                     YAKDEVDIIDQYVAKNDDGKWITDDKGNPKIEDTSKLAELNDFLDELASEPVVIKGGE
                     YSKRFIDFLEYLAESEDEFTSEEIIIIDNILEQFEESKGE"
     gene            22912..23523
                     /locus_tag="VGY24_000639"
     CDS             22912..23523
                     /locus_tag="VGY24_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ4283643.1"
                     /translation="MRNWKVTGKYPQFDSTGAVASTHIIITAEDGSVISQPIKQDLTS
                     TNDTEIIKATLEEFKKSEYVEIAMGEAVQKVDDLEKISQETANTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGKVEPDIYKGMLELIEPAKQGEYQAYDVFTVVDESHEDQ
                     AGEGNLVFVHVNEPFTYDKQTLKELESEDKVTVIKYADLVKQD"
     gene            23533..23988
                     /locus_tag="VGY24_000640"
     CDS             23533..23988
                     /locus_tag="VGY24_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEQ4283644.1"
                     /translation="MIIDLTSLIHLFGDLVRTVEIHVFTLFVCFDIITGLTKGITNKR
                     ANSTKGLSGIIKHFLVVLLVYTVYPYLIFLGAKPLAVAFVLFFIACYGISIVENWGQL
                     GLPMPSFVRSFFEKLKRDTDQFDIATIKIDKTGVKLEAPQVDLKQKEEE"
BASE COUNT         7969 a         4223 c         5417 g         6442 t
ORIGIN      
        1 ttgaaaaaaa acgtaaccct gacctttatt gaccaaagga ggaaggatga ggactgaaaa
       61 agatattgaa aattatttga aaaagaaaac aaaagggctg tgtttaaaat tcaccagtcc
      121 agggacgata ggagcgcctg acagaattgt tgtcatgaat acgggaatct tttttgtaga
      181 ggtcaaagcg cctggtaaaa aaccaagacc cagccaagtt gccatgcaca aaaaaataaa
      241 agaggctggg cagcacgttt gggttgttga ctcctacgaa tcagtggaca tggccttaaa
      301 agaaatggag aactgggtgt gagactgcac gagtaccaag aatacgctaa gacatggata
      361 gtagagcacc cttattgtgg ccttttactt gacatgggcc ttggtaaaac gctgacaaca
      421 ctatcggcga tagatgagat tcaaaatatt ttttccgagg atcataagat tttaattgta
      481 gcccctaaaa aagtggcgga aaaaacgtgg ccaacggaga ttgagaaatg gcattttgat
      541 ttcacctact ctaaagtttt gggggacgag agaaaacgaa ttgaagcgtt agaaacagaa
      601 gccgatatct atttgattaa tcgtgagaat gttacttggc ttgttgaata ctacaagact
      661 aaatggccgt ttacctttgt ggttattgat gagttatcaa gttttaagtc tagtaagtca
      721 aaacggttta gggctttgcg aaaagttaga ccgaaagtcc aacgccttgt aggactaaca
      781 ggaaccccag cgcctaacag tttgattgat ttgtggccgc agatttatct gatggacaga
      841 ggcgacaggc ttgagacgag ccagactcga tttaaagaca agtattttgt tcctgataag
      901 cgtaatggtc caatcattta cagttgggca cttagggatg gtgcagaagc agaaatctat
      961 aacaagattg aggacatctg tgtcagcatg aaagctaaag actatctcaa gttaccgccg
     1021 cgaaccaaca acgttgtatc agttaagtta tctaatatga aagcctacaa acagcttgaa
     1081 gctgatttgg tgttggagtt taaaaataaa gaaatatctg cggctaattc tgcggttttg
     1141 gccaataaat tacttcaaat ggccaatggt gctatctatg atgatgataa agcaacagtt
     1201 gctatacacg acgacaaact tgacgcgctt gagaacatag tagaagaaag ccaaggccag
     1261 cctatcttag ttttttacca gtatcaacat gatcttgaga gacttaagaa acgatttcct
     1321 caggctgaag agctgacgtc ggttgacaag tggaattccg gaaaaatacc aattcttctg
     1381 tgccaccctc aatcggcggg gcatgggctt aatctacaaa aaggcgggca tattattgtt
     1441 tggtttgggc taacatggag tcttgaatat tatcagcaag ctaatgccag attagataga
     1501 cagggacaga cagaacccgt tattgtgcac cacattgttg cagaaaatac agttgatgaa
     1561 aaagtactta ggattttaca aggcaaagaa aaaaatcaga acgccttact tgaagcagtt
     1621 aaggcgcagt taggagtcta gatgaaaaaa gaatatgtcg ttagaatcta cacgggtaga
     1681 gaaaagaatt ttgaggcaaa acctcagttt gaagagaaaa cctttactcg aaaaccagac
     1741 atgttgaagt tttggaattc ttgcgaagca acagttaaag aaaaatatac tagggagcgg
     1801 gagaatgact gaagaacaga tgattgattg cttgctttat gagttagtaa aaaaagacaa
     1861 agcaatcaaa aagaaaaaca tcattatcac tgcactaaca gttatgctgc tgattgtcgt
     1921 atcagggctt ggcatatcac ttaaaagtta ctacgagccg caaatatacg gactacgtgc
     1981 tcagctaagt aggacacaaa agcagcttaa acgtgctagt gatgatagag ctagacagac
     2041 aaagagaatt gcagaattga cgaaaaatgg gggataagga tggacagatt tgataaagca
     2101 caagcggcag cgtttgactt gatgtggatg tgttctggtg gaaaatccag tcgaacattt
     2161 aaagcggagg ggaagacgta taagatgact ctggaggagg taaaacttag tgagcagatt
     2221 atctaatgca cagttaaaag cttttgacga atggctgttt gattatcgtt ttattgataa
     2281 caagatcgca ctccgaaaac ttgagctaca aactgatatg aatactgcgg tagataaaaa
     2341 tatcggtgga ggaagagcaa attttgtttc aaaagttact gaggatgtgg ttgcaaggtg
     2401 ggattcagat agggaactaa agggtctatc caatttcaaa gaagcagtta ttgcaacgtt
     2461 aaatcttttg gatgaagaac taactgatat ttttaatctt aggtggggcg ttggttcaag
     2521 taacacttgg gaagaaattg cctatacaag acatctgtcg cgtaagtcta tttaccgcaa
     2581 aagagagcgg attctagaaa tattttccaa aaaaatagga atgtgacaca aaaatgtatg
     2641 gaattgtcat ctttttgatg ataaattggt agtatgagtt tgaaggtaag gcagatagtg
     2701 tcttgccttt ttgctatgct tggaggtgat ggaaaattga gtaaattaac cctaaaacag
     2761 aagcgttttg cagatgagta catcatctca gctaacgcga cagcagctgc tattaaagca
     2821 gggtatagta aaaagacagc aaggtcaata ggtcaagaaa acttgaccaa acctgacatt
     2881 aaagcttata tagacgagcg gcttgaaaaa cttgaatctg aaaagattgc tacgcaagaa
     2941 gaagtcctac aatatctcac ttcaattatg cgaggagacc aacaagaaaa gacgctcatt
     3001 agcgttggag agtttggcca gaagatagtt gacatcgatg ttggtgccaa ggatagaatc
     3061 aaggcagctg agctactagg caaacggtac aggttattta ctgacaaggt tgaaatggat
     3121 gtcagctcag atgtaaccat taacgtaggt gagtgggatg acgattaaac agcgccctaa
     3181 aattaatatc gtaatcaagc atcctagcaa agtttttaac aaacatatct acgacaaact
     3241 ttacaactat agcaacttca cggaagttca ttatggtggg gcgtctagcg gaaagtctca
     3301 cggtgttttt caaaagataa ttttaaaagc acttaacccc aaattcaaac atcccagaaa
     3361 gatattagtt ctcagaaaag tcggtgcaac tgttagagac tctgtatttg ctgatatcat
     3421 gtctaatttg tcgtatttcg gcatcttgga taaatgtaag ataaatatgt cggcttttag
     3481 aataacgctc ccgaatggct cggaattcat ttttaagggt atggataacc ctgagaagat
     3541 taagtcaatt aaggggatat ctgatgtcgt catggaagaa gctagtgagt ttacgcttga
     3601 tgattatacg cagttgactt tgcgtttaag ggataagaaa catctagaga agcaaatata
     3661 cttgatgttt aaccctgtgt caaaagtaaa ctgggtttat aaagccttct tcgttaagac
     3721 acctaagaat acagttgtct atcaaacaac ttataaagac aaccgcttct tagatgatgt
     3781 cacgagagaa aatattgagg aactagccga taggaatgaa gcttactaca aaatatatgc
     3841 tcttggccag tttgctacac tcgataaact aatttttccc aaatacgaca agcaaatatt
     3901 aaacaaagac aagttatcac acttaccttc tttttttggt ttggactatg ggtttatcaa
     3961 tgacccttcg gcacttttgc atgttaaaat cgatgacgca aacaagaagt tatacgtctt
     4021 agaggaatat gtcagaaaga atttgaccaa tgacaaaata gcaaatgcta taaaggacct
     4081 cggatatgcc aaagaagaaa tcagaggaga ttcggctgaa aagaaatcta accaagagct
     4141 gaggaattta ggtattccta gaatgattga tgttaccaaa gggcctggaa ccattatgca
     4201 aggaattcag tacctgcttc agtatgattg gattgttgat gaaaggtgtg tcaagactat
     4261 tgaagaactc gaaaattaca cttggaagaa agacaagaag actaatgagt atatcaacga
     4321 accagttgac agctataacc actgcattga cgccataaga tatgccgtac aagacagaat
     4381 ataccagtcg gcggacagaa gtaagcgcat gaaaaatgct aaatattatt tttagaatgg
     4441 ggtgagactt tggaagaaaa acaattttta gcagggactc gttttaacga gaatgctaat
     4501 aggcaattta ttatgctgca agaagacttt gaagcaattg actatgaatc aaaactttgg
     4561 atagatcaac ttaagaatta catttcaagg tttaaagcag aacaattaga gcgtttgaaa
     4621 gagttaaagc gatactatct tggggataat aacatcaagt atagacctgc taaaacagat
     4681 aaatacgcag cggataatcg tattgcaagt gattttgcta agtatattac ggtatttgag
     4741 cagggctata tgcttggtgt tcccgttgaa tacaaaaatg aaaataaaga cctccaggca
     4801 gctattgacc ttatgtcggt cagaaacaac gaggactatc acaacgtcaa aataaaaaca
     4861 gatttatcaa tttatgggag agcctatgag ctgctaactg ttgaggaaat agatgacgaa
     4921 aaaaccgaag taaaactcta tcaactccca gccgagcaaa cttttgttat ttatgatgat
     4981 acataccagc gaaattcatt gatggctgtt catttctacg acatagatta tggctcaggt
     5041 aaacgcaaac agattaccaa agtatatact tccgacacaa tttacaccta cgaagactat
     5101 aatcttgaaa caaaaggtat gcgcttaaaa gattatgaag aacatttttt taaaggtgta
     5161 acagtcaatg aatatgctaa taatgaggag cgtacagggg cttatgagtc tgtacttgat
     5221 aatatcgatg cctatgattt atctcaatca gagcttgcta attttcaaca agattcagtc
     5281 aatgcgttgc ttgtaatagc tggtaatgct tacacaggag ctgatgagaa tgactactta
     5341 gatgatggac gactaaatcc taatggtcgt ttagcgattt caattgggtt taaaaaagcc
     5401 caagtgttaa ttttagacga taatcctaat ccaaatggcg ttaaaccaca agcgtacttt
     5461 ctcaaaaaag agtatgatac cgccggtagc gaagcctaca aaaatagatt agttgcagac
     5521 attttgaggt ttacttttac gccagataca caagatatga aattttcagg agttcaatct
     5581 ggtgaatcaa tgaagtataa gcttatggct tctgacaact atcgcgagaa gcaagagaga
     5641 ttgtttaaaa aaggcttaat gcgacgctta cgcctagcag ctaatgtctg ggccgtcaaa
     5701 gggaatgaag caactacata tggccttgta aatgatacga gtatagtttt cacacctaat
     5761 cttcctcaaa acgataatga aattgttaca gctgcacaaa atctttatgg tatcgttagc
     5821 gatcagacta tctttgaaat cttaaatact gtcacaggag tagatgctga agcagagctt
     5881 gaacgtttga aagaagaagc tgataaaaag caatctttac cagagcctag attggtaggt
     5941 ggtgctaatg gtcaagaaga accaacagca gaaaagcctt aattactggc aaaagcgtca
     6001 agaagatatt ctaagctatt tagacagagc cgatttagat gtcttttcag aactgcagaa
     6061 gctatatagt gaacaggctt ttgagttaca aaaggaattg tttgattttt atactaagta
     6121 ttctgaagag aacaaaatga cttatcagga tgtcgttaaa aaactacgtc atgaagattt
     6181 atcagattac gtagctaatg ctaacaagta tcgtaaacaa gctgagaaag atcctgagct
     6241 gctaaaacga cttaatgagc agtatgtatc agcaagagct acaaggatgg atgcattaaa
     6301 tcttgagctt gtttatcgtg cggggatact aaaaggtgta cttgattcag catttgaaaa
     6361 tcatttaaaa aaagctgcct catatgctta taaaaaagca atgggtgggc ggtcagggac
     6421 aatcaatggc ccagttttag aagaactggt tagaaccccg tttgatggtt ataactattc
     6481 agaacagttg tggggcaata ctgacaatct tgttaagaat cttcaaaaga gattaaaaca
     6541 aggttttgtt cgtggcgagc atcctagagc gatggccaga gatttagcta agcgatttaa
     6601 tgttgctaat cacagggctg aaacactaat cagaactgat ggaactatgg tgattaataa
     6661 tgctaccgct aggcgctact tgaatgcagg acttaagtat tatcgtgatt tagttagact
     6721 tgatgacagg acaactgaga tatgccgtac aattgctaaa gaaaacaaaa gaaagctatt
     6781 atctgagcta aagcctggaa ttaatgtagc accctatcat ttcaactgca gaacaactat
     6841 tattcctgat gaagatgagc taagtattga agtagaacca attgatgata aaagcactaa
     6901 gtattttaag gatgtcacct cagattggat agatggaaat gagcataaac cacaactgtc
     6961 actactaaat gagtacgtaa aaaatggcac tccttacaaa gtagatggcc atagtgtcgt
     7021 tcttgatcat tcgaattatg aatatagagt tgctaattgg ctatccaaaa agacaggatt
     7081 gcaagttgac atggtgccaa gagttaattc ccctgaacat atcaagactc ctgattattt
     7141 agttgatgga gctccttttg atctaaaaga aatcacaggt tcgggtaaaa acgtcattga
     7201 tggcaatcta cgaaaagcaa aaaagcaggc aactaatatc atttttgata taacaaaaac
     7261 ccctttatca tttgaagaga taatggggca attagaacat atttatatga tagatcgtag
     7321 gggtcttgat atatcaatta tcaaaaataa agatgaggta ttagctgtat tagaaaaaga
     7381 aggaggatga cccaccgcct ccacagtaaa ctgcttcatg ggcgttagac catcatcctt
     7441 ctttatctca attatacatc accctattaa aactatcaag gaggaaaata tgtttatttg
     7501 gcaaatggta ttagtggcac taggtgtatt ggtactcata ttgattgttg gcattgcagc
     7561 aatagcggtt aagtcaatta ttgcagaatt gaaaaaagaa ggataaaaca atgaataagc
     7621 gcattaagaa aaaacgtaaa ttggaaacag cggttgtgat gcttgttgca gagaatgcca
     7681 tgcaagcaga agctattaaa aatcaaaaca aacaaatcat ggagctaaaa tcaatcgttc
     7741 aacgaaacgc tctggcaaca aacgaagagt tagcgactgt taaagccgct actttagata
     7801 accaatcagt tatcaaggca attggtgaca cggttgacta tattaagaaa aactacaaac
     7861 ggaagtgggg gaaataaagt ttaaccgtgt cgaattcgac ccctttagaa accaaagtcg
     7921 tagcaatacg gctttttatt gtgtccaaac tttgttgatg acactaaaag ctacactgtt
     7981 tcgtcgcagg acgtaaagct agactatcgg ttggtggcgt aaccactagg agaaagttat
     8041 ggcagaagaa aatgtaacaa cagagacaac tgagcaagtc gacactcaaa aagaagctgt
     8101 tgaacatcct aagcatgaat atgagcgaac ttttactcgc gcagacatct caaaaatgat
     8161 ggctgctgaa cgcactaaat gggaatctga acaatccgca gctattgaaa aagctcgtac
     8221 agaaggtgaa cgtttggcca agttgtcaaa agatgagcgc gctaaagagg aggaacaaaa
     8281 acgtctagat gctatcgcag agcgtgaaaa agcagtagca gagcgtgaga tgcgcattga
     8341 gacgcattcc ctgcttgtgg agaaaggatt gccattggat tttattgata ttgttttagc
     8401 cactactgca gaagaggtta aggccaatat tgataattta caaactattt ttgataaagc
     8461 tgttgaaaaa cgtgttaacg atcgtttaac tcagaaaccg ccacgaactg gaaatggctc
     8521 ggtcggtatg actaaggctg acatcatggc gatagaggat gacgacgaac gtatgcgttt
     8581 aattgctgag aatcgtaact tattttaaga ggggaatatt atggctgaaa aaaacttaaa
     8641 cactatggcg gacttaggag atattaaatc aattgatttt gttaacaagt tttccaaaaa
     8701 tattaatgat ttactaaaat tgctaggggt tactcgtcgt gaaacattaa ctaacgatct
     8761 aaaaattcag acctataagt gggaagtgac tttagatcaa actgatcctg gagaagggga
     8821 aacaatccct ctgtctaagg ttactcgaac taaagataaa gactatacag tgaagtggtt
     8881 caagaaacgt cgtgcaacta cagctgaggc aattgctcgc catggtgcag ctcgtgctat
     8941 tactgaagca gataagcgca ttatgcgtga gcttcagaat ggaattaagg atgcattctt
     9001 tacattcctc aaaacaaaac caacaaaagt taaaggcgtt ggccttcaaa aagcgctgtc
     9061 tgcatcatgg gctaagctag ctacttttaa tgagtttgag ggttccccgc ttgtttcttt
     9121 tgtctctcct ttagacgtag ctaactatct tggagatact aaagtaggtg cggatgcctc
     9181 taatgttttt ggaatgacat tgcttaagaa ctttttgggt atgcaaaatg tgattgttat
     9241 gccatctgtg ccagagggta aaatctactc aacggctgta gaaaatctag tttttgcatc
     9301 tttaaatgtt aaagggggag acttgggcgg cttgtttgct gattttactg atgagacagg
     9361 tttaattgct gcagctcgta atcgtcagct ttctaatctt acctatgaat ctgttttctt
     9421 tggggcgaat gtactgtttg ctgaaattcc tgaaggggtt gtagaggcaa caatcgaagc
     9481 tgctgctgta cccggcattg gtggttaaag gctatttata tgggtgataa acaacttatt
     9541 gacgatatca aactctttat aggtatttcc aagggtgatg gtgcgcaaga tgagatcatc
     9601 acccttgcta tatatgaaag taaagagcgt gtgctagcta aacttaatga atactcagaa
     9661 actgaaatca ccaaaattcc tgatagattg aggtttattg tccgtgatgt tgctattaaa
     9721 cggtttaata ggattaattc agaaggagcc gttgaagata gcgaagaagg aaagactttt
     9781 aagtgggaca gttaccttaa agagtatgaa tcaacactca gaagcgctgc tattgggaag
     9841 gtatattcag gcaaaggggt agcaagattt atttaggaga tacacaatga tctataaaga
     9901 tagagtaatc ttagtgtatg tcgatgaaca ggacgatttt ctagataaaa gaactgttga
     9961 aaaacctagc gggaaaatcc cctgtatgga aaatactttc acaaaatctg aacagatggg
    10021 gctatttggt aaatatgatt tgaatgcttt taagttgcac ttgcaaggcc attacgacgg
    10081 ctttagcaag attatctaca agggaaaatc gaggttgata aagggactag cacaccataa
    10141 aaatagcacg gttatttatg tatgagtctt atttatcgga tgagaggcct agataggttt
    10201 ttacgcagcg ttgagcgcaa gcagaagtca gtacgaatcg ctgtagataa agagcttagt
    10261 aaatcagctg ctagggttga gagacaggct aaaatactag ccccggttga cactggatgg
    10321 ctgagagctc aaatctacag tgagcaacaa cgactcttac actatagagt ggtttctcct
    10381 gctttatatt ctatttatct tgaattaggt actcgtaaaa tggaagcaca atcgttttta
    10441 gaccctgccc tgagaaaaga atggcctgtg ctaatggcta atatcaaaaa aatgtttaag
    10501 aggtgatgca tggattactc actggaaaca ctatatttaa aaaaggtaaa aaatagattg
    10561 ggagttttag acataccaat ctattttaaa ttgcctaaat cagacgtttt agagcctttt
    10621 attgttgtag gtacaaatat atcagacttg tcaaaaacag ctcaaactgg agcagttatt
    10681 gatgatttta gcctgaatat cgatgctttc ttacctggcg atagtcgttt ggatgcagaa
    10741 gagataaaat ctcgcatgct tagactgctt gggcgaaata accaaataaa ggctcagatt
    10801 ttggtagata attcaatagg acgagaagtc tatagagttg ctatcaacat tacagaaaca
    10861 ctattttaaa ggggacctaa atggcaaaca caaaaaaagg aacaactatt gaaattacaa
    10921 caggtaaacc gattgtagga aaaaagattt tttactttat acaatcagtt gatgcaccaa
    10981 agaaaagtca ggctctgttg ccagcctatc gtaccgatgg tactacaacc atgggtggtg
    11041 aatacattga tgagcaaacc caacaaggtc gcgttattga gaaggctaca gatgaacact
    11101 ctattgattt gacaacgtac tttgtaccga cagatccatc tgttgctgtc atcgaggaag
    11161 ctaagaaaac aggtaaatct atcaaaattt gggaagttat tgccgacgaa agtgtcaaag
    11221 aacagattca aattcctgaa tcgactggac ccaagaaaga tgtttaccct gctaagtttg
    11281 ggtacgccaa gattgacgaa atcgaacgcg gtactggtat ttcagactta gtagaaatgt
    11341 cctacaccgc taacattgta ggggccttac aagatggtaa attccctctc acgaaagaag
    11401 aaattgaaat gctagaaaat gtatacggct atcaaaatcc gggtgatact actggcgact
    11461 acgataatat cactaaatag cacacagggg tggcaactgc taccccattt tgatttaaaa
    11521 aggagataaa ataatggaat ttacagcagc aaaacgaaat atcgatatta aatttgattt
    11581 taaaactatg tttaagatca ataataaact aggaacaatt aacccagaaa caggagagcg
    11641 caatgcagat ggtgttggtg ctttgttttt caatatctta gagcgtaatg aaagcgccat
    11701 tgttgacctt gtgcgtttat ctgcgggaag cggaaaaaaa gcgctaactg aagatgaaat
    11761 tctagatgca attgcagaag ctgttgatga agaaggaaca acagaagggt tgtttgctga
    11821 gattgaaaaa gaaatggttg attctggttt tttcagagcg aagattttga aatatatcga
    11881 aaacatggag aaatcagctc gttatctcaa agcgaaggac gatatggacg caactcaaat
    11941 ccaaattatc gaagacatga ttggaagaat gagcaacgca gtatcttagt aaattgcgca
    12001 cggctagggc tcacagacat tgatactatc tataaatgta ctaagtggga gctagaggct
    12061 attatggagg ggcttgagta taagcagctt gctgagcgcg agagcctctc tgaattgtct
    12121 ctaaagctta gatatacatt aaatgctaaa aaggttgatg taggtaagct caaatacgat
    12181 aagcataggc taactatcaa aagatcgtat caaaaagcta gccgtagcca agccgatagt
    12241 gatagcagta ttgttgagag aatacaaatg cttaataatc attttcaaaa tagatagata
    12301 aggaggagta gatgccagga acgtttgatg gttctatttt cgctgatgtt ggtgccaaca
    12361 caaaggacta tgagcaggcc atggcccgta ttgttagtac gactcagaat gctttcagaa
    12421 aagcccaaga tatagcggtt aacagtagta ataaaatggt tcaaatcatt ggacaaatca
    12481 tggcccaatt agcgaacaac ggcgaatcgc tcggaaagag acttggttcc gcctacgcta
    12541 ctggtttaaa actgagcatt ggtgaaattc aacgtatagc agcttccatt ggcgaaaaaa
    12601 ttcccgaacc cataaagaat gggtttcaaa aaatcattat tccagttgca agtgttattg
    12661 acaaaatacg gggtaactta actggtttca ttaatcaaac aaaaatgcaa cttcagaatc
    12721 ttgtggatat tggtaagata agatctgcat ttaccaaggc tgcgactgga gttgatatcc
    12781 taactcagag ggcaagtagt aagcttaata acttatctgc aatattcgaa acggtcgcag
    12841 gcaagcttcc gcgaccattc ggaacagcgt ttagaaaaat tgcctcatcc gtttctaccc
    12901 tgaatagctc tatacagtct gttggaggga agatttctaa ttcacttggt cagcaagtct
    12961 tgaacccggc cttacagtca tggaataatt tttttagttc tgtatcagct aaggcatctg
    13021 ctttcgcaaa caaagtgagt actagtttgt ttggtcggct gacctcaagt ttagctaatt
    13081 tatcaagtaa aatagggagc agtcttagca atggttttag cagaatgtct agttctgcgg
    13141 ctacttcctt gaatggaatc agccagaaat tcgccaacac ctcttctgct ggagagagac
    13201 tcaaaagtac agtgatgagc atcgtgcagg cctttagctt aatggctgtt gctcaaaaag
    13261 ctatgcacgc tattactggg gcaatagacg gggcggttag tcgcgttgat accatgaacc
    13321 gctttccaaa aacgatggct ctgtttgggt attctgctga acaatctaaa gcatcaattg
    13381 ataagctatc aaaaggaatt gaaggtctgc caactcctct agacagtgct gtaaaaagtg
    13441 ctcaacagct cgccataacc acaggaagtt tagataaagg gactagttta gccctcgcct
    13501 tcaataatgc aatgatcggt tacggggcaa caactgaagg ggctgagcaa gcactcaggc
    13561 agtttaacca gtcgttgggg tctggaaaaa ttcaagctga agaatttaac tctgtatcag
    13621 aagctgcgcc aggtttaatg tctaaaatgg cggaagcctt cggttttggt aaaaatggcg
    13681 tacaagattt aaaatcagcc ttatctgatg gcaaaatcac tgcacaggag ttcgcagata
    13741 aaatgattga gcttaacgat gcccaaggcg gatttgcaga gatggcccaa tcatcggctg
    13801 gtggaatccg aactgcgtgg aagaacgtac ataccgccgt tgtaaaaggc gtagcaggta
    13861 tgatttcagc ctttgatgaa gcggctaaag ctaatggtat gaagaccatt gctgaaacac
    13921 ttcttagcct gaagcccgca ataaccagcg tttttgatac gattaattct cttattccaa
    13981 atgcagttgc agcattcgct aggctaaaac agtctatcaa tattgacttt agcccattag
    14041 gcgccagcgt taaagaggtg tttgctctta ttaatactgt tcttggagat tttgcacata
    14101 ctggtgaatt atcagggcag acttttgata agttaaaagc aaaaattgct tctttagccc
    14161 ctaaagtcat tgccctttgg gcagtgatga accctgctag cgctatagca acgataatgc
    14221 ctttgctttc tctattcgga aaagttggtc tagctttagg aagcttaggg acttctgtcg
    14281 gggcgtttgg cggcataatt tctagtggga tagctagtgc cagcggcgtt gttggtgcct
    14341 ttgcggcaac tctaagcgga ttacctggcg tttttgctac cgcagcaggg cgtgggttat
    14401 ctgtggttgg aactatgaca agcgcaatgt ccagtcttgt gagtttagca ctggcggcta
    14461 taggaccggc cgctattcta ggtcttgtgg tggcaggact tggtttgatt aatagtcagt
    14521 ttggcgcaca aatcgaccag ctacttaaca ccgcagttac aaaaggtcct ggtattattc
    14581 aaggccttgt aaaagggatt acttctaaaa taccagcttt aatagctagt ggtactcaat
    14641 tgattgctaa atttgcaaat gctatcacag tgttattacc agttataatt caagctggcg
    14701 ttcagctaat tacgagtctc gtccaaggta ttggacaaaa tgcaacgaat ttaattagtt
    14761 cagcaataaa aatcattggt agttttgtta gttcaattgc gagtgcattg ccgcagctta
    14821 tttctgttgg aatggagcta ttattaaatg ttgtcaacgg catagttcaa aatatacctc
    14881 ttattatcca gcaagcccag caaattattg atagttttgg gaatagttta caggctaacc
    14941 ttcctagtat tattagcaac ggtatagcta ttttggtgaa tcttgtacag ggaattactc
    15001 aaatgttgcc aactgttctg cagatagcta ctcaagtcat tacgagtttt gtatctggaa
    15061 ttgtacagtt tttaccgcag ttacttcaag gtggtattca aatcattatt agccttgtgc
    15121 aggggattat tcaaaatcta ccccagattg cacaatctgc tgtacagatt atacagtttt
    15181 tagcttctgg actgacgcaa gctctacctc agattatcgc agcaggcatt cagcttgttg
    15241 cgcaactagc cgtagccttg ataaagggac ttccacagat tatttctgcg ggtattcaat
    15301 taattatggg gctcggtaaa gccatgttag gggctatccc aaacgctctt tcaggagttt
    15361 gggaaggtat taagagcgga ttcagttcga tgtgggacca aatcacaggt aagagttcca
    15421 caagtacagc taaagtctct gcggacgcca cggtcatggc catgaatgtt gggatgcaaa
    15481 cgacagctat ggctaatcaa gctaatacag atacgacatc tatgcttaat agcattagtc
    15541 aaaatacaga gcttgctaat ataaacgcta catcaaatgc cacacagatg gctaataacg
    15601 ttaatgctca gacaggaacg atgagtcttc aggggttgaa tgattcgatg tctcttgcta
    15661 gtggcattag cgctaatatg tctgtcgcta atataaacgc tactactcag gctcgagaaa
    15721 tggcttcagg ggttaatggt gcgacttctt caatgaatct agacgccatt aatcatacgc
    15781 tcagcttagc tagtggtgtt ggtgcgaaca tgggagctgc tagcacaggt gcaacttctc
    15841 aggcgcaagc tatgagctca ggggtgagta acagcctggc atcaatgcag tcaaactcaa
    15901 ctaaagcagc ctctggtctg tctaatagtg tgacaagcaa aatgtcttct gcagcaacct
    15961 ctgcaacctc aagcgttaat aggttgtcct cggcggtcga gtctggcttc aataaggcga
    16021 agacttcagc aacaacctca atgaatggga tagccaatgc ggttaaaaca ggatttagta
    16081 gcattaacag tactgcaaag caatctatgg caaaccttgt aagttcagtg acttctggga
    16141 tgtctcgtgc aaccgcggtg tctaataatg cttgtcagaa gatcctctca atattcaggg
    16201 ctttggcagg gcagatgtct tctgcaggag cttatgcagg tcaaggtttt gctaatggtc
    16261 tagctagtag cgcaggcaca atctacgcaa tagctaacag cattgccgct aatgtagcag
    16321 caacaattag acgcgcttta gatattcact ccccatctcg tgtcactaaa acactaggtg
    16381 catttactgg agaggggttt gccctcggta tggcagagtg gataggtgag atcaatagcc
    16441 taggtaaagc atacgcaaca gcagttactg atcaaaactg gggggtaaat agtactgttt
    16501 caacatctgc taaagttaat aatagtggaa tcaatacctc tcttgataat cttagcgaag
    16561 aggttaggca gtctcaattg tcagagccta tctttgaagt gcataatgag attgttggag
    16621 ataaaattta cacggctgtt aaagaaaaag aatcaagaga gcaatctaaa gattcttatt
    16681 ttgtttttga ctagaaggga gtattttgga tttattaata gaaaaagaga gtcaggcaac
    16741 taggttgtct gactttggta tttataatat tgctattgaa gatagcgctc ctttactatc
    16801 tgtatcacac cgagcagtca aaggaaggag tggttacatc tacgatgggg ctacttttac
    16861 gactaaaacg cttaaggtca aaggaagagt gacggtaagc aacgtagaaa gacttttgga
    16921 caaacaggac gaactaaacg ctctgcttgt tgttgacgaa cctttctacg tgactaagat
    16981 gtaccctgaa aactcagatc tatttaactt tgagcttcca ggggaaagca caggagacct
    17041 gcaacttatt ggctctcctc ataaaccttg gaaatataga tttaaagtca tcttagacga
    17101 cactatcgct tatgaattta taggaaaaac cagtcaagga ctaaaatata atctttcctt
    17161 cgcgctaaga actgctgaat tacctttcgg tgagacaaag ccaaaggaca taactctatc
    17221 agggggcagt tttgcctatg caggaaccgc taaagcaagt cagctagagt ggccttttat
    17281 tatagagctt actccttctg gaggtcagac taatttttat attgaaattg atggcaggcg
    17341 ctttgagttt aagcagaata gccagctaca aaatagtgat aaattacttt tgacaggcat
    17401 agctaccaca ctaaacggaa attatgtcaa tgctaaaacg aactatgagt attttatctt
    17461 caatcctaat cctaacaaga gaattactta caaaacggat tttcttggca cgattaggat
    17521 tttaaatttt gtagagttgt ataaataggg aggtctaatg attacttttt tggatcatcg
    17581 tgacattgag tatggggtca ttagtgtcat taggcatact aatgctgtta atggtgagcg
    17641 ctcagtgagc ggggaaattt atacaaacag tgatgtcctc aataatatcg atagagggtg
    17701 gcgacttaga tttgaagatg agtattacgt tgttatttac gcaaagcctg ttgatgttgg
    17761 tcaaaaaacg caagtttcgt ttgatgccat acaccaattt ttttgggact tcagcaagag
    17821 tagtatctat gagggtctcg gagatgggtc tcataccatt gatacctatc tagagacagt
    17881 atttaaaggt agtgggtacc gctataagct agaagtaggt gtgaacgcat ttagaaaaca
    17941 atcttttaat tataagtcca ggttagattt attcaatgag attattaaag cgaccggact
    18001 tgaattttca gtcagcggga aagttgttcg gctattaaaa aacataggta ctgatctgtc
    18061 aacagtggta cgaaaaaatt tcaacatgaa tgaccttacc attgaaaaaa atattgatag
    18121 ctttattacc tatcagaaag gctttggtgc atggaccgac ccagaggacc actctaaggg
    18181 gagacttgag gcggaatacg aaagtcctct tgccaaggag tacggtcgac ttgaaggggc
    18241 gcctctgacg gatgagcgct tcacggtggc agataatcta aaagaagctt taaaaagtaa
    18301 tgttgaaaat tcatataaga tatcagtaaa aattgatatg gaagatttaa cgagagctgg
    18361 gtatagatgt gagaggccgg ttgctggtga ctacattatg gcgataaatg agactttagg
    18421 ttttcaagag cgtatacgga tagtttcttt tacaagctac tacgacgcaa ctggagcttt
    18481 agtaaaacat gaagtcactt gtaatgatat cggatctgtg aaaaaacaaa gcgtaggaag
    18541 tctgtctata aacagtagaa tcaatcagat agatgcagat attgcttctg caattgaagt
    18601 agcaacacaa gcactagtat ctgctgacgg gaaaaatact gtttacggtg gcacagaaat
    18661 gcccaaagat gaaccaaaag gaaccttaaa aaaaggagac atattattcc taaaagttgg
    18721 cgatactact agaatgtatt tctggaatgg tgctgagtgg gaagaacctg aggttgtgaa
    18781 cgacccagaa cgatggcgtg aagacctcga aaaacaaatc tctgaggcga tcgaaaaagc
    18841 caaaaaagca caggaagaaa tcaaccagcg caccgataaa gagcttgaag aatttagagc
    18901 taccctcaaa aacttagcgt taccagagga agcgattaaa aaaatcacag aggctatcaa
    18961 agttgatgac atcccgtcta ttaaacaaag ctttgatgac ctcaaaaata gagtgagtga
    19021 gacaagcgaa gaatctcgtt taactgccga aattttagga aataacggta agacccgcta
    19081 taacaaaaat ttattggttg gcgaccctaa tcgtgttaaa aaaattgatg aggattacat
    19141 cgaggtagaa gccaacgatg gtggttttaa acgtggcgag acatacacga taagctttag
    19201 ccagacttgt gagctactcc aaaaagtggc tatcacgctg acacaggcta acaacaaggg
    19261 agttaagtta gtgctgacac caactaaagc aaaaatggag tcgcagacct ttgacctcac
    19321 taaagataaa gaggttatca gtgtttatcc gtttagctac acagtgcttg taaccagcga
    19381 ctggtataaa tctaagcaga tagatttaaa cgcgtcggag gtgaaggaat tggctctgga
    19441 gatggattat aaagagattg cagatgccaa aggtgcaact atcacagggg catggtcaga
    19501 cagtccacaa attatattag acggaggtaa aaaatgagtg aaaatatacc gctgcgagtc
    19561 caatttaagc ggatgactgc tagcgagtgg gctcgtagtg atgtcatctt actggagagc
    19621 gagataggct ttgagacaga cacaggtttt gccagagcag gtgatggcca caatcgattt
    19681 agtgaccttg gatacattag tccactcgat tacaatctac tgactaacaa gccaaatatt
    19741 gatggattag cgacaaaagt cgagaccgct cagaaactac aacaaaaagc agataaagag
    19801 accgtctata caaaagctga atcgaagcaa gagcttgaca agaaattaaa tctcaaaggt
    19861 ggcgtcatga caggtcaact aaaatttaag ccagccgcca ctgttgctta ttcctcgtca
    19921 acgggtggag cggtcaatat tgacttgtcg tctagcagag gtgctggtgt tgttgtctat
    19981 tctaacaatg ataccagtga tgggccgcta atgagcttgc ggacgggtaa agagaccttc
    20041 aatcaatcag cgctttttgt cgattacagc ggtaagacta atgccgttaa tattgcgatg
    20101 cgccagccaa gcacacctaa tttttcctct gcgcttaata ttactagcgg caatgaaaat
    20161 ggtagtgcga tgcagctacg agggtcagaa aaagcgctag gaacgctaaa aattactcat
    20221 gagaacccaa gtattggagc ggattatgat aaaaatgcgg cagcgttatc cattgatatt
    20281 gtcaaaaaga caaacggtgc aggaacagcc gctcagggaa tctacattaa ctcaacctca
    20341 ggcacgacag ggaagttgct taggattaga aaccttagtg atgataagtt ctacgtcaag
    20401 tctgacggtg gtttttatgc caaggaaact tcgcagattg atggcaacct gaaactcaag
    20461 gaccccacag cgaatgatca tgcggcaacc aaagcttatg tagataaagc aatttctgag
    20521 ttaaaaaaac tcatactaaa aaaatagatt aaggaggata aatgagcaga gacccaacac
    20581 ttattttaga cgagtcaaac ctcgttattg gtaaggatga acgtgtgcat tacacattta
    20641 ccgcagagga cgacaaccca aaagtcagac tagctagcaa gtgtctaggc acagcgcact
    20701 ttaatcagct catgattgag cgaggggata aagcgactgg ctatgtggcg cctgtggttg
    20761 ttgagggtag cggtgagtcg acaggtgtgt ttaaaagtct tgaggagatg cttagtggtc
    20821 tacagtctat taatttagag ctgacagata ctaaaaactc caatctttgg tcaaaaatca
    20881 agctgacgac aaacggcatg ttacgtgagt atcatcgtga taacatcaca acggaaatca
    20941 tcgaaaaagc tgatggtatt gccacacgga ttagcgagga ttctgacaaa aagctagcgc
    21001 tcatcaatga caccattgat ggtatcagac gtgagtatca agatgctgat agggagctat
    21061 ccgcaagcta tcaggcaggc atcgagggac taaaagccac aatggctagc gataaacttg
    21121 gcttgcaagc tgagattaag atgtcagccc gaggcttatc tcaaaagtat gatgacgaga
    21181 tacgtaagct atcggctaag atcacaacaa cctcaagcgg cacgaccgag gcctacgaaa
    21241 acaagctcga gggtttacgt gctgagttta ctcgtagtaa tcaaggcatg aggatagagc
    21301 tcgagtctca aatcagcgga ctaagagctg tacagcagtc aacagctagc caaatctcac
    21361 aagagattag agacaggaca ggagcagtca gtcgtgtgca acaagactta gacagttatc
    21421 agcgtcgttt gcaggatgcg gaagataatt acagtagctt aacccataca gttagaggtt
    21481 tgcagagtga tgtggtatcc ccgactggta aaatccaatc acgccttact caactagcag
    21541 gacaaattga gcagcgggtt actagagatg gtgtcatgag tattattagt ggcgctggag
    21601 acagcattaa attagctatc caaaaggctg gcggcattaa tgccaaaatg tctggtaatg
    21661 agattatctc agcaattaac ctcaactcct acggagtaac aatcgcaggt aaacacatcg
    21721 ctctcgatgg caatacgact gtcaacggta cctttaccac aaagatagca gaggctatca
    21781 agattagggc tgatcagatt attgcaggca cgattgacgc tgctaggatt agagtgatta
    21841 accttaacgc aagtagtatc gttggtttag atgctaactt tatcaaagct aaaattggct
    21901 atgctatcac tgatttgctc gagggtaagg tcatcaaagc tcgtaatggc gctatgttga
    21961 ttaatttaaa caactctaac atcgacttta acagcaatgc cacaattaat tttaatagtg
    22021 gcaacaatgc cttagtacgt agagatggca cacatactgc ttttgtacac tttggtaatg
    22081 cgacacctaa aaattatgcg ggaagtgcgc tttatgcagc cataggtata acctcgtctg
    22141 gtgacgggat taacagtgcg tccagcggtc gttttgcagg gctaaggtca tttaggtacg
    22201 ctacgggata taaccatact gctgcagtcg accaaacgga gatttacgga gatagtgttt
    22261 tagttgtgga tgactttaac atcaatcgag gatttaagtt tagaccagac aagatgacaa
    22321 aaatgatcga tatgaacgac ttgtatgcgg ctgtagtagc tttaggacgt tgttggaagc
    22381 acttagctaa cgtcggctgg aataccgttc atggcaattt tgtaagtgct gtgaatgggg
    22441 aattgaataa ctacatcaca aaaatttaac aggagataat atgcaattaa ctattaaaaa
    22501 caaagattta aacacactat atggtgtact agacaaaatc aaaatcacta atatgcgtgc
    22561 taatcgtggc cgtgctaagc tacttgcaaa agtcgagggc aagctaagcg agtatgccaa
    22621 agatgaggtt gatattattg accagtatgt tgcaaaaaat gacgatggca agtggattac
    22681 agatgacaaa ggtaatccaa aaattgagga cacctcaaag ttagctgagc ttaacgactt
    22741 tttagacgag ttagcaagcg agcctgtcgt tataaaaggt ggcgagtact ctaagcgctt
    22801 tatcgatttt ttagaatatc tggcagagtc agaagatgag tttacatcag aagaaattat
    22861 cataatcgac aacattttag agcaatttga agaaagtaaa ggagaataaa catgagaaat
    22921 tggaaagtga caggaaaata cccacaattt gacagcacag gagcagtcgc aagcacacat
    22981 attattatca ctgctgagga tggctcagtc atctctcaac caatcaagca ggacttaacc
    23041 tcaactaatg acacagagat tatcaaagct actttggaag aatttaaaaa atctgaatac
    23101 gtcgaaatcg ctatgggcga agccgtgcaa aaagtggatg acttagaaaa aatctcacag
    23161 gaaactgcta atactgccaa gactgctcaa acagctgcag gattagctaa ggtgtccgca
    23221 gagcgtacac agcgaatgat taacttgcaa accatccacg tattgacaag tggtgggaaa
    23281 gttgaacccg atatctacaa aggtatgtta gagcttattg agcctgctaa acaaggcgag
    23341 tatcaggctt atgacgtgtt tactgttgta gatgagtcgc acgaagatca agcgggagaa
    23401 gggaacctag tctttgtaca tgtcaacgag ccgtttactt atgacaaaca gacgcttaaa
    23461 gagctagagt cagaggataa agtcacagtc attaagtatg cggacttagt taagcaggat
    23521 tgaggtggtt agatgattat tgatttaaca agtcttattc accttttcgg ggatttagtt
    23581 cgtaccgtcg aaatccacgt ttttacgctt tttgtctgtt ttgacattat tacaggactg
    23641 acaaaaggta ttactaataa gagagcaaac agtacaaaag ggctatctgg cattattaaa
    23701 cactttttag ttgtattgtt agtctatact gtctatcctt acctaatctt tctcggagct
    23761 aagcctttag cagtcgcctt tgtcctattt tttatcgcct gttatggtat ttcaatcgta
    23821 gaaaattggg gacaactagg tttgccaatg ccaagttttg tcagatcatt tttcgaaaag
    23881 ctcaaacgtg atacagacca gtttgatatt gcgacaatca aaattgataa aacaggtgtt
    23941 aaactcgagg cgccacaagt tgatttaaaa caaaaagaag aggagtagca tgagacaagc
    24001 agtcacacga ttagcattag ttatagcaat cgcaatactg tatgtgccat t
//
LOCUS       DAUCWU010000016        23491 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUCWU010000016 DAUCWU010000000
VERSION     DAUCWU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23491
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.016"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..546
                     /locus_tag="VGY24_000641"
     CDS             <1..546
                     /locus_tag="VGY24_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4283645.1"
                     /translation="SKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVID
                     NITSWQSDWFIEQGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPTNIFVTAWESTQ
                     DLTLESGQIITQYVPDIRKQVLSQTLGLTDIVGRIQVNPKTGGHGILLQGSDGLYAKN
                     RLDNRTVCKAEELFNFEGSDA"
     gene            582..1985
                     /locus_tag="VGY24_000642"
     CDS             582..1985
                     /locus_tag="VGY24_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4283646.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGHVLFL
                     VHRKELIDQIINSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QIIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKIMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWSIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECPVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QFANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            1990..2472
                     /locus_tag="VGY24_000643"
     CDS             1990..2472
                     /locus_tag="VGY24_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEQ4283647.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNDRNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQQYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPTSFHVWKEKATGFTQAEIEEDDLPF
                     "
     gene            2490..4043
                     /locus_tag="VGY24_000644"
     CDS             2490..4043
                     /locus_tag="VGY24_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEQ4283648.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGAYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSMVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESIFRK
                     YWLAFTDRDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHALSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
     gene            4312..5181
                     /locus_tag="VGY24_000645"
     CDS             4312..5181
                     /locus_tag="VGY24_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEQ4283649.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDGINYTQKIGIMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEAFKPKQEGITTYFGGSVTYTSNGSHEGR
                     EAYERVATGTSYNRNNDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            5135..5485
                     /locus_tag="VGY24_000646"
     CDS             5135..5485
                     /locus_tag="VGY24_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ4283650.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGHMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKKFMEVMASRGALVGVAR
                     SVEDALNIVNDTSR"
     gene            5469..5826
                     /locus_tag="VGY24_000647"
                     /pseudo
     CDS             5469..5826
                     /locus_tag="VGY24_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6066..6350
                     /locus_tag="VGY24_000648"
     CDS             6066..6350
                     /locus_tag="VGY24_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEQ4283651.1"
                     /translation="MLTEDTFKKIEELEAACQDTTDNIKKSSHYQGRHGMEAIDVVKN
                     FSACPEHEEGFYWGNAVKYLLRYHAKNGIEDLKKARQNLDWLIEKLEEVE"
     gene            6347..6760
                     /locus_tag="VGY24_000649"
     CDS             6347..6760
                     /locus_tag="VGY24_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283652.1"
                     /translation="MIPNFRAFNQKIQKMYGVDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLDDYILMQSTGLFDKNGVEIFEGDVVKLQYTITSDLDFFKVNQFRGGSWR
                     IDNRRRGSDLWLRNEDCEVIGNIHENQDLIESVEE"
     gene            6757..6963
                     /locus_tag="VGY24_000650"
     CDS             6757..6963
                     /locus_tag="VGY24_000650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283653.1"
                     /translation="MRKYIEFKDEWKSAADHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     REGFSPQNKKTSTRNAPLVKFKLK"
     gene            6987..7427
                     /locus_tag="VGY24_000651"
     CDS             6987..7427
                     /locus_tag="VGY24_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ4283654.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLALNRVSAEQELDAIERAVNGIFDPEYRLILIDKYLLTYPKTD
                     CDIYTKLGYEKSQYYNMLDNALLSFSELYKEGILLVEKLEKSWN"
     gene            8145..8483
                     /locus_tag="VGY24_000652"
     CDS             8145..8483
                     /locus_tag="VGY24_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ4283655.1"
                     /translation="MRPQKLTISGGRRTTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKLWRETSKQVLLQNDYVCAMCGGEATMTDHIISVKKDWNKRLDWNNLQASCKACNDS
                     KAIRERRKNN"
     gene            8654..9121
                     /locus_tag="VGY24_000653"
     CDS             8654..9121
                     /locus_tag="VGY24_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEQ4283656.1"
                     /translation="MGRNLKLVETTKKHLTKEEKIVRETAQNKASDGLKKLQKTPPEH
                     FNNVAKYEYRRIIEDLQNLPLRNLDRGLLELYCTWYAIYKETSRKLDEVGYFTNDPDK
                     GIIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKPKSIFDKFGG"
     gene            9124..9354
                     /locus_tag="VGY24_000654"
     CDS             9124..9354
                     /locus_tag="VGY24_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283657.1"
                     /translation="MERVKEFFVKLVENVKFPIVEINGEKINGITKLIVKYDSDDAQN
                     NPLDNGFLVEYISEEGGRYYMQTIGQSFRIGL"
     gene            9358..11112
                     /locus_tag="VGY24_000655"
     CDS             9358..11112
                     /locus_tag="VGY24_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEQ4283658.1"
                     /translation="MKVEYDYSAISDIYKDDAFYYAKMVVDEQIKSSKKVFRACLRHL
                     NDLKKIDGDNFKFIYLPEKAADPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLSSLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIV
                     DDGYFGYVAEQDNEEEIKDESNWIKSNPILEVEALHDKLMDYLRTRRRVSLETGEINK
                     VLIKNFNMWRQSSEESYIDKTTWELARIDKPDTNKRRVWLGVDVGRVSDLFAITPVVM
                     MDDFWYIDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EEMVYSNDWEINGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAINNAYIKTDNNGMRIDKNKNSNKIDPLDAALDGYAVCYLEPF
                     DGSGYWTNEKIIGGESLF"
     gene            11109..11279
                     /locus_tag="VGY24_000656"
     CDS             11109..11279
                     /locus_tag="VGY24_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283659.1"
                     /translation="MIDFILKNIHTLILLAGLGLLMYGLFLFGDKVGFIASGLILIVL
                     AIYVDSVGGKRE"
     gene            11272..11496
                     /locus_tag="VGY24_000657"
     CDS             11272..11496
                     /locus_tag="VGY24_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283660.1"
                     /translation="MNKRIKKKRKLETAIVLLIKEVAELRSIVSANAKATNNELAAVK
                     SAILDNQVAIKSIGDEVGHIKQNYKRKWRK"
     gene            11530..12750
                     /locus_tag="VGY24_000658"
     CDS             11530..12750
                     /locus_tag="VGY24_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ4283661.1"
                     /translation="MSFFRPLGSSKVSYDDYISSVLAGDVSQKYLGVSALKNSDILTA
                     TSIIAGDIARFPLVKKDVNGDIIHDEDINYLLNVKSTKNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTNQALQFQFYRPSETTVEETDSHEIIYTFTDTLTAKQVKCFAHDVVHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSSGILTMKGAQLSGDARQR
                     ARQEFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSELGLKTLNDKDRRLYRIEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLIELGKQKSTDPNMDRYQSSLNYVFLDKKEEYQDKVGI
                     KGKGGEVNAKEDKS"
     gene            12728..13393
                     /locus_tag="VGY24_000659"
     CDS             12728..13393
                     /locus_tag="VGY24_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEQ4283662.1"
                     /translation="MPKRINLKGPLIANNSQEVYDYYGMEATSAKSIINQLPEDSSDI
                     ILEVNSNGGLVTVGSEVYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNLVCGNSDDLDKASNALKSSDKAIVNAYVAKTGLSEDEIMNLMRNETFMSA
                     QDAVEKGFADEVMSFEAVASIDNQMLPQAVIDDYYANRSKRKQEISNMLLEIEKEEIL
                     QGL"
     gene            13418..14605
                     /locus_tag="VGY24_000660"
     CDS             13418..14605
                     /locus_tag="VGY24_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ4283663.1"
                     /translation="MFEEKIKEIKATIASLNQAIATKTAEVKNALESDDLETARSIKA
                     EVEQAKADLVEAENDLKLYEASIEKGGAENTGGKEVPQETKTYRESVNEFIRSKGTVT
                     NEALRFEGKDEVLIPLNQTTPIDPKTDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHPAKKASGKWPVLQCATEKMVSVEELEKNPKLAKPKFKDVEWKVETYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIAKVLKTFEPKADVKTLDEIKKILNVDL
                     DPAYNVSFIVSQSFYQSMDTLKDENGRYLLQDSITSVTGKVFLGKPIFVLADEVLGKN
                     KAFVGDFKRGVLFADRKDLGLRWVDNDIYGQYLQAVLRFGVAKVDDKAGYYVTFTPEQ
                     LPS"
     gene            14750..15052
                     /locus_tag="VGY24_000661"
     CDS             14750..15052
                     /locus_tag="VGY24_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ4283664.1"
                     /translation="MAVSKELLDSVKLYCKIDFDFEDDIIKEMIESAQEQICFAIEEG
                     STADTFKESAKFALAVKKQVKEEYDHRGLSADSFRYPLANGVLNIIHQLRLRGDDS"
     gene            15049..15396
                     /locus_tag="VGY24_000662"
     CDS             15049..15396
                     /locus_tag="VGY24_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEQ4283665.1"
                     /translation="MITRKMNVRITIFSQSGGQNEDGEVVSAIRKDLYTCWAEVLKTQ
                     LRDFNYQSKFQNASNLPTNKDTKVFLIRYNPQLSIDNTMFVEFNKRIYKIDKIESDES
                     GKDITMISGVSMS"
     gene            15393..15770
                     /locus_tag="VGY24_000663"
     CDS             15393..15770
                     /locus_tag="VGY24_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ4283666.1"
                     /translation="MTRGLDEILANLTKLEVKAPKTAKVAVTKVAKEFEKTLKSNTPV
                     YEIETDERLQEDTVISGFKGSNVGIVSKEIGYGKATGWRAHYPNDGTIYQRGQDFKER
                     TINQMTPRARQIYAEKVKEGLGL"
     gene            15767..16192
                     /locus_tag="VGY24_000664"
     CDS             15767..16192
                     /locus_tag="VGY24_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283667.1"
                     /translation="MIAETAAYKLLSNDKTLNELLDRLRGGTFKNGFKQGIFTYDIPD
                     NPVNLRKVELAPFMRINTTLDGPFDYADDEILCNEQRITINFWCKTASEADQINKCID
                     NILKQGGFERYTANEKPRYKDRDIDLLMNVRKYRYFDFY"
     gene            16208..16816
                     /locus_tag="VGY24_000665"
     CDS             16208..16816
                     /locus_tag="VGY24_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4283668.1"
                     /translation="MGKVKFGLRDFYYGVLDGNDKVTKAESIKHLPGMKSAKLDITNE
                     LVTVPADDGPYVVLSGGISETKLEIELLDLTSDARKDFFGITVEKGVEKYNKNLTPND
                     IVCLFRTSDENGKAIWIALLKGKFNLPGMEAQTKEGAPDPKPDTTTGNFVARGPKEEV
                     LLVGREGNSGFNLNEFLKCVFAGCGDAEIKKIPIAEQASTVM"
     gene            16869..17195
                     /locus_tag="VGY24_000666"
     CDS             16869..17195
                     /locus_tag="VGY24_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283669.1"
                     /translation="MYEITLKKGGVDKEFKKDFINVEDNLLAVEHQVRQSAVFSDEKR
                     RLDSKAHRKLNESYLQMFVDMYAGQFTVDDLKQSDMTVLNTLNDLYIAALGGEQEEDE
                     SEKKEQ"
     gene            17243..17392
                     /locus_tag="VGY24_000667"
     CDS             17243..17392
                     /locus_tag="VGY24_000667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283670.1"
                     /translation="MKNGYTILDIKKMRLSDIELMVQALEIETVEKEEVIETTLDKAF
                     PFLFG"
     gene            17405..21328
                     /locus_tag="VGY24_000668"
     CDS             17405..21328
                     /locus_tag="VGY24_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEQ4283671.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTSMKGLDNSLKAVEKSFQ
                     GHGGRIKGLKAVYAETGSALKGYQELLKTQSQKYSDLKKEIGDVNNATAEQKQKLIGA
                     KSVMLETAAQVAELQNRLRALATETSVFTRFGKAAERIGGKMKSFGDSVAGVGAAFTR
                     GVTAPIVAGAGYAIKAAVDYESAFAGVKKTVDETATVSYAKLSQGIRQMAKELPASAV
                     EIAHVAEAAGQLGVKTGDILSFSRTMIDLGESTNLSAEEAATSIAKIANITGLASSEY
                     SRFGSAVVALGNNFATTEKDIVAMTNRIAASGKLAGLTNQEMLALATAMSSVGIEAEA
                     GGTAMTQSLSAIERAVASGGDNLNKFAQIANMSSADFARAWKEKPIVALQEFIKGLGQ
                     LDKQGESATKVLDELGLSGIRQSNMLKSLGLASETLGKALGISNKAWKENTALTDEAN
                     KRYETTESKLKMLKNEVNDVAIEFGGPLVDALRNGLEAGKPIIQMAADLAKQFNSLDK
                     EQQQQIIKWGLIAAAAGPALSILGKGIGVIGGTIQAIGKMSKGIGALSGWLRTFKAGA
                     VAASVGAEAATASMGSLTGAVALLSNPVTWGVLLGGAVVIGIGLIADSMYKAQKRTEE
                     WGTAVSATEATALSNFKKKVDETNTSLQMFEAGAGSVKKVTEAFDDLVGSIEKLAQSK
                     LDKNINLAKKLGLSEETINALKSKTESVVNNVKSMNTQIKAIMEKHNGDMSQLSSAEK
                     ELVLRNQREMIIAQLDLIKFSASEKKALTAALNNELDAINARQLEKVSENTVKMLDKE
                     NSAYKTKKAELKEILKQFGSDTSKLSAEELAARQEVLNRLTELNMQHNLKTKALNDQY
                     LAIQRERVQRLKESGKSQEEIHRGISQMASDMAQKLGISYDDAYRKMAYYTEKSGETL
                     KVLSRNTANATAEVAAANAQWDSLFTSDNPQQSLNELLSTAEGWNSFEIMVKNADVEP
                     TGRAALAEMLVAGGQWQNMTLEQKKLVVDGQQAMIEIFDSKELLAQWQALTPEEKVLL
                     AKNLTQEPTMSAQQALDSVKQTVPADVNATDKTAGDTQSAQSKIDNVKQKAPADVKAS
                     DKTRPDVASANRAVNSPKQNSPAVIRAQDNASGVAENVIWSLARIPRSVTTTITTFVR
                     KIFGHEKGTDFHPGGLAVVNDQKGALYRELVTLPTGESFIPTGRNVILPLPRGSKVLK
                     ASRTKQLFPHYANEIGFDDTKIASLTTRLKSVQDKGTVVVNADPQLTELIKLLKDRDD
                     RNVTNNYTLNATNSSSSEDIFSQKNMRRLLRELAYYTKGEEGRLA"
     gene            21328..22035
                     /locus_tag="VGY24_000669"
     CDS             21328..22035
                     /locus_tag="VGY24_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4283672.1"
                     /translation="MRYIEFNGTKSDSLGLLLERERSIKSTSNDVNLIEVDGRDGVLI
                     KDNERLKAVEQDFPFSLVGDVAVNQQKISEWLHVKGWHDLALSWDKDYIYRASVVNLF
                     EIDEILKQFGRLKVNFLIHPIKYLKTGKQEVPLVNGDTLQNPGNVQAKPILKIKGTGD
                     GVLTINGFETGLENVQRELVIDMERHLVYKDVLSAWDNIVRTKRHRMPLFDVGQNTIS
                     WTGNFTITAVPNWGVKV"
     gene            22032..>23491
                     /locus_tag="VGY24_000670"
     CDS             22032..>23491
                     /locus_tag="VGY24_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ4283673.1"
                     /translation="MIPVLYEAKETRFRTFGLGEIADAYEVKVTRERNGNYSLYIKYP
                     LDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIFARHISMRTQDIALK
                     PSVSGSSVGAESALEIWKENLVGDDTFDVKSDILTLGSFSWEADKIGNARGALGGVAG
                     SILDVYGGEYEFDNRTIILRKQMGRKAPTVLEYGRNIVSVEEERLLDGNYTSIYPYVR
                     YTPQPKPQEEAPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYAQRRIQMVDLSSHFN
                     DDKNKKEPTVEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTLDYQDFRVMEEVELC
                     DIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSISKSLTGGVFERINAL
                     ENNQKVITNNQKQFELNLPKYLNDINGKRVWYEKPDDNIEHKIGDYWFEKNGKYQRTW
                     IWDGHQWVKVLDTEDLNPNQRAFDEAMAEIEKAKKA"
BASE COUNT         8118 a         3883 c         5181 g         6309 t
ORIGIN      
        1 tctaaaacga ttgccggaaa cgaaaacatt gatattatcc aatttgacag gacgcaccct
       61 agcgacttta tgaccgaatt cttgacagaa ctaccagaac ttatcaaaga atatgacaat
      121 ttagtcattg ataatataac aagttggcaa tcagactggt ttatcgaaca aggtcgtaaa
      181 tccaaaaatg gaatcacaaa cgaattgcaa caatacaaca tgtggaccaa ttactattta
      241 cgagtattga ctaccattta cagccaacct actaatattt ttgtgactgc ttgggaatca
      301 acgcaagatt taacgctcga gagcggacaa attatcacgc aatacgtgcc agatatccgc
      361 aaacaagtcc taagccaaac gctaggttta accgatattg tcggacgtat tcaagtaaat
      421 ccaaaaacgg gcgggcatgg aattttgttg cagggcagcg acggactata tgcaaaaaat
      481 aggcttgata ataggaccgt ttgtaaggct gaagagttgt ttaatttcga agggagtgat
      541 gcgtaacggt ataccattta cacgaatatc aaacagaact tataaatgag gcaagaagac
      601 atattttaaa gcataatgtg atgattgtta gtccacctgg aagtggtaag tcagtagtca
      661 tttctgatat tgctaagtca gcgactcaaa aaaacggaca cgttttattc ttggtccatc
      721 gcaaagaatt aattgaccaa atcattaata gttttaaatt ccatggaatt gacatgaata
      781 aagtggattt aatgacagtt ggtaaagcta agaaccgctt ggataaatta acaaaaccaa
      841 ccttaattat cacagatgag ggtcatcatg ggaaagctag tacctaccaa ataatctatg
      901 aatacttttc agacgtgccg aggattggtt ttactgccac accttggcgt ctatcaggcg
      961 acggttttac agacacttac gatgttatgg ttcttggtaa aacggtcgag tggcttatca
     1021 ataataataa acttgcacca tatgattatt acagtgttct atcaattgat actgcgaaat
     1081 taaaagtaca aaacggagat tactccaata aatcgattga cgaatcattt ggtaaaaaga
     1141 tttttggtga tgtagttcaa gaatatataa aaaaagcgaa cggtcagaaa gctattttat
     1201 acgctcactc ggtagaagca tcgcaggcat ttgctaaaga attccaatct atgggaatta
     1261 atgcaataca cgcagatgca aaaacgccta aagccaaacg ggataaaatc atgaaagatt
     1321 tccgtgacgg taagatacaa gttatctgta acgttgattt gatatcagaa ggtttcgatg
     1381 tcccagattg tacagtaact attctttgta gacctacaaa atcattagta ttatttttgc
     1441 aacaatctat gcggtcgatg agatatcaac ctaataaaaa agccatcatt ttagatcacg
     1501 taggaaactg gagtattcac ggcttacctg acacaccgca tcattgggag aattatttcc
     1561 gaggagggtg gaagaagaag tcgaataaaa ctaacacggt acacgcaaaa gaatgtcctg
     1621 tgtgttcggc tttgtggcca cttagtcaac agctctgtga attatgcaat catgattttg
     1681 gattgaaaga aaaacaagag aaagaacgca tagaggcaga acttgaactc ataaaacgtg
     1741 agcggtttag aatcaaacaa tttgctaata agaagtttgg taaagattta aaaacaaact
     1801 gggaaattgc ccaagctaga gttaaagacg ctggtaaagg aaaaccatta tataaactta
     1861 tctatttcta cttaaaaact gattgggtag aaacaaacgt taatgaactt gccgaagtaa
     1921 caggcaagtc agaaaaagaa atatatagcg cctacaattg gctaaaaaag aaattaagag
     1981 gataaaaaca tggcaggatt tacaacagat ttttcagaag ttaaagaaca cgcagaattc
     2041 aaagaacaac catacgaaat gattgtctat gatgcatatg aagcagtaaa tgaccgtaac
     2101 ggcaaaaaac gtgttgttat tgactacgtt gttcgaaacg atatcaagca agaaatgcaa
     2161 aactaccact tatgggatga gcaatatccc aactcacaaa ctgggaagta tcatatcggc
     2221 atcttaatgg gtaaagcaaa agcacttggt atcaaagagg ggcaacaata cgatagtttt
     2281 gaagcgtttt taaacgactt caaaggacgc actgcaaaag taaccgttaa acttgacgaa
     2341 tataacggaa ataaataccc gaaagttcgt tacgcaaatc aaagcgatgt gcctaccagc
     2401 ttccacgtat ggaaagaaaa agcaactgga tttacacaag cggagattga agaagacgat
     2461 ctaccgttct aatttagact gggggataaa tgacatcaaa tgaatttatt gaagctttat
     2521 caaaattgac aaccgaaacc gattggggag acccaatctt cggcgagtca gtgcttaaag
     2581 ctgaattacg aaaacactta tttaaaattg tcccaattga tcacaatggg tatatccaca
     2641 aactgtttta ttcagaaatg gttaaagatg aagatgtcat gtattttgtg tcagatggac
     2701 gaaaaactta tcgcttttta tttggagata caattctaaa gactgataaa caaggcaatg
     2761 aatatctcac atactccgtt gaaaataatt ttccaccatt tgcaaaacta gttatcgact
     2821 acattctagg tgcttacacg ttttttgaga ataaacttta tgacattcga tataagcaat
     2881 ttaaattgat tgatgatttt acacttcaga ctaagtatgg tttcaaagac tctggtcaca
     2941 ttttagagat actacaaggt atccacaaaa cattaaacat ccaaccaatc aattatattg
     3001 aaccatatca gattgcttgc aaggatttca taatcgacct tgaaaattct gaaatcatta
     3061 accaaccgcc tttgcaaaac gtatcttatt ttaagtatta cgaagtagac tacaaaacag
     3121 caataaacag taagtctatt gcagaagaat atcttgagta cgttattgca gatagcaatt
     3181 cgttaaacaa tgcaatacta caatcttatt ttatcgccca agtagcgtgt ggtgttagac
     3241 ctaaaaccaa cttcttcatc tcaaaatctg gagtaaggac tggtaaaggg ttaaggcata
     3301 tagctttatc tggtctattc aataagattg atgttgagct agatacatta aaaagtaatg
     3361 gatttgaagc gttacaggcg tgggcgatgt tctcgggtgg agaaatggct ctagcgacgg
     3421 aacagggaga tattcaaggc aatgcaatgg agcgtgtact taaaattatt gcaacagaga
     3481 aaacacacgt tgcacgagca attgggcaaa atcagtcaat ggttaattta acgagtgttc
     3541 tgtgtattga caccaaccgt actgttgcgc tgtcggatga aatgaatgga cgaaaggttt
     3601 taattcagtt taaagataga ccaaaaactg aaactgatta cgaaagagag agcatattca
     3661 ggaaatattg gctagcattt actgatcgtg ataaaaatcc aaaaatcgat ggatgtatcg
     3721 gttttctgct aaattcactc gagcgtttcc aaaaaattgg taaatggtat caatggaaag
     3781 atgttgaagt atttaatgat atcgatttag acgaatttca agttgcttta ataaatgcat
     3841 tacaagaagt tgattttgta cagcgaactg ataacaaaga agttattgac ttatcattac
     3901 aagtttacgg aaaaagcaat catgcattaa gtaaagccat atctgaaatt ggtgtacgta
     3961 gcaggtcgaa gaaagttaac gggaaaacag tcagagggta tgaaatcgaa aataaaacac
     4021 gttttgataa atatatcctt taaaaaggta acgcatggtc acgcaaagtt acgcagaatt
     4081 ttccatgttg cgtaacctac tcaatctctt tagtaccaat cgatttcagc atttatttaa
     4141 gaaaggttac gcagtaacaa acaataagtc taattaatga tttattaatt atttatttat
     4201 atataggggt agggtagggt gattttgcgt tactgcgtaa ccatccttct ctaatgctat
     4261 ctatatcaac gttttagagg ttacgtagta aaaaaggggg tgcgttacct tatgtaccac
     4321 acaacagcac tttcattctt aaaaaaagga tatcaggtta taccgcttag aaaagatact
     4381 ggaacgccaa tgattaagtt taaagatatc caaataacgg aagaagtgat caaaaataca
     4441 aactggttta attgtgatta tgctttatta atgcgtggca tttggtgtat tgatattgat
     4501 actcatgaca tggacgagaa gttagctaaa gagttataca taatgataaa aaagatgggg
     4561 attgatttat tatctgtatt atcgactgat aagtatgaca atggactaga tggttattcg
     4621 tcaattatta ggcatgagta taaaaacgaa ttaatcagta attttaaaaa tacattcgca
     4681 gaattaactg caagtggtgg tatgcacata ctctttaaaa aacgcgatgg tattaattac
     4741 acgcaaaaaa taggaataat gcctggtgtt gacataaaag cgaatgataa taattttgtc
     4801 aaaatattcc catctgacgg acgtgaagtt ttacaagcag ttaaaacatt accttattat
     4861 gatggaaaat ttgaagagga agcatttaaa ccaaaacaag aaggtataac aacctatttt
     4921 ggaggttctg tcacatatac atcaaacgga agtcacgaag gccgagaggc ttatgaacgt
     4981 gtagcaactg gaacatcata taacaggaat aatgatttgt ttaaaggagc gtgttgggcg
     5041 tttgaaaatg gtatcgatat tgatgattta acatcaatta ttgggacggt taaaggcaga
     5101 gacgtattta cgagagagga gtttgaatta acgattgaat cagcgaaacg aaaagtcagc
     5161 tacgtcacta tcagaacatg atatccaaaa ccttatccga atggaattat cacaagcggg
     5221 gcatatggta tttcgagcaa atgtcggaaa ggtgaaaaca gcagatggta gattttttga
     5281 tacaggttta ccaaaaggtt tttgtgattt gtttggattt aaaccaaacg gacaaatatt
     5341 ttttatcgaa gttaaaaatg aaacaggtcg gataagacca gaacagaaaa aatttatgga
     5401 ggtcatggca tctagaggag cgcttgtagg agtggctcgg tcagtggaag atgccttaaa
     5461 catagtcaat gacactagta gatgattttt acaaacaaat ggagccgtca attaaagcgt
     5521 ttttagacga taacattacc atcgcagaca aagaagaagc tgacagagtc tatagatctg
     5581 tcaaatacta taaaaaacta aacagattgc cgccacctga tgtattggag tggttccaac
     5641 gaatctacac gacagaggaa atgataatgt taatcaaaca gtcttaccgc cttaaacaaa
     5701 aaaaagacag atgaggatga caagatttac gaaaagtgga tgtttaaaaa ctacggtgac
     5761 gttaagctcg ttaaaaaaat caaacgcata aacgcattag ctaaggctcg ggagatgggt
     5821 ctatgaaaag acacagacag tggcataacg atattaaata tacacagatc ttacgataat
     5881 ctgttgcctt acgatatatc agagctgtta atagctcaca gatgcaaaat aaagacgtct
     5941 gatgacgttt tagcagatag gataggtatt tatacttggc aattaaaagc gctcttagaa
     6001 cgcagaatat tgccaaatga gagcgtgtgt aaaacgatag taaattattt gagagaggta
     6061 gagagatgct gacggaagat acgtttaaaa aaattgagga gcttgaagct gcttgtcagg
     6121 atacgacaga taacattaaa aaatcatcac actatcaagg caggcatggc atggaagcaa
     6181 tcgatgtggt taaaaacttt tcagcttgtc cagagcacga ggaaggtttt tactggggca
     6241 atgctgttaa gtatttacta cgatatcatg ctaaaaatgg cattgaagac ctcaaaaaag
     6301 caagacagaa ccttgattgg ttaatcgaaa agttggagga agtggaatga taccaaattt
     6361 tagagcgttt aaccaaaaga tccaaaaaat gtatggtgtt gacggcttta aatcaagtga
     6421 acgcaaaata tacagatgca gcttagcaga tgatgagttt cgctctggtc gcttagagac
     6481 gtttcatttt gtcgaggata accttgatga ttatattctc atgcaatcca cagggctatt
     6541 tgataaaaac ggtgttgaga tttttgaggg agacgtcgtg aaattacaat atacaattac
     6601 tagtgattta gactttttta aagtgaatca attcagaggt ggttcttggc gcattgacaa
     6661 tagacgacgc ggatcagatt tgtggttaag aaacgaggac tgcgaagtta tcggtaacat
     6721 acacgaaaac caagatttaa tagaaagcgt ggaagaatga gaaaatatat tgaatttaag
     6781 gacgaatgga aaagtgcagc agaccacctg aacgatttta tcgacaaaaa caagtacgca
     6841 aaagtgacag ttgttggtta tcaagtcgta caactttctc cttacggaag agagggcttt
     6901 tctccacaaa acaaaaagac gtccacacgg aacgccccct tggttaaatt taagcttaaa
     6961 taaattatac catattgggg gctttcatga cgttttttcc tgagattgat atccaaaaaa
     7021 caaaatccaa tgccaagcgt aaattgagag agtatccacg ctggcgaagg atagctaatg
     7081 acgtagatac tcaaaaagtt acagctactt actcatttga gccaagacaa ccgcatggag
     7141 ttcctagcaa accagttgag agactagcgc taaatcgtgt gtcagcagaa caagagctgg
     7201 atgcgattga gagagcagtc aacgggatat ttgatccaga gtatagattg atactgattg
     7261 acaagtattt gctcacatat ccaaagactg attgtgatat ttatacaaaa cttggttatg
     7321 aaaaaagcca gtattacaac atgctagata atgctttatt gtcgttctca gagctatata
     7381 aagagggaat tttgcttgtc gagaaattgg aaaaaagctg gaattaatat ggaataatta
     7441 tgtactttat atataattat tcatgttatt atagtactat caaaataaca agaagagata
     7501 accttttaac cactggctat tcatttagtc gccaacttta actacgatca aacttgttat
     7561 tttatggtat gtgggacgtg caggttcgaa tcctgccgtc tctatcagcg tcaggggaga
     7621 cgcgacccca cacccattta aaggtagaca cgatctgatg tgtggggttt tatgattaac
     7681 cgcaagtaaa acaagggtcg caaccttgct tgtggttagt agacctagtg gtcgtttatg
     7741 cacgttcgat tcgtgcaagg tctgtttgta gctatgtacg gataaataat agccttagtg
     7801 tccttcggga gctaagtcgt actaaatgct acaaaattgg aacatgaacc gtgattggaa
     7861 aacggtggag gtagcgcctt gcttcgggac gttagctaag tccgaaaact ctttgcgaag
     7921 agccagaaga agatgtgtcg gttcgattcc gactgttcct gtaacaagtt agcttaaagc
     7981 gtaagtaatt ggtagacgta ccgaggaggg gcgcatgcgc aaagcgctgg gctgataacc
     8041 cagagatgga ggttcgaatc ctctgcttgt ggtaaaacag accgacatag atgtcgggca
     8101 gaagtcatca catagtggtg gctttttatt atggaggtta gggcatgagg ccacagaagt
     8161 taactatctc tggtgggaga cgtacaacgg ttgactacga cgatagatca gcggagtatc
     8221 gtgactacaa tcgtaatcgc tggaagtacg ataagcaagt caaacagttt tataattcta
     8281 agttgtggag agagacaagt aagcaagtct tgttgcaaaa cgattatgtt tgtgctatgt
     8341 gtggtggaga agctactatg actgaccaca ttatctcagt caaaaaagat tggaacaaaa
     8401 gattagattg gaataacttg caagcaagct gtaaagcgtg taacgatagt aaagcgatac
     8461 gagaaagacg taaaaacaat taatagaaaa acgggtgtaa aaattaacaa cactatgtta
     8521 atgttcggga aataccccgc ttgtttttga acggggctat attgttcgga aacttaagaa
     8581 cgcgcccttt tccgtgcaaa aaattccctt tttgaaattt ttaaaactgt aaagttcgtg
     8641 taaaggaggt cttatgggaa gaaatttaaa gctagtcgaa acgacaaaaa agcatcttac
     8701 gaaagaagaa aaaatagtgc gagaaaccgc gcaaaataag gcgtctgacg gtcttaaaaa
     8761 gttgcaaaag acacctcctg aacactttaa taatgtagct aagtacgagt ataggagaat
     8821 catagaagac ctccaaaacc tacccctaag aaatctcgat agagggctat tagagttata
     8881 ttgcacatgg tatgctatct acaaagaaac aagtagaaaa ctagatgagg ttggttattt
     8941 tacgaatgat ccagacaagg gcattattcc tagtccactt attttaacac tagaaaaagc
     9001 cacagcgaat attagaagta gcgcaagtca gcttgggttg actgtggata gtcggatgaa
     9061 aatgtttatt cctaaagaag aggaaaagcc taagagtatt tttgataaat ttggaggata
     9121 aaaatggaac gagttaaaga attttttgtt aagctagtag aaaatgttaa gtttccaatt
     9181 gttgaaatca acggtgagaa aataaatggt atcactaaat taattgttaa gtatgattca
     9241 gatgacgccc aaaacaatcc gttggataat ggttttttag tggagtatat tagcgaagaa
     9301 ggtggtcggt attatatgca aacgataggc caatcattcc gtataggttt gtaggtgata
     9361 aaagtggaat acgattattc agcaatcagc gacatctata aagatgacgc tttttattat
     9421 gcaaaaatgg tcgttgacga acagataaaa tcaagtaaga aggtatttag agcttgttta
     9481 agacatttga atgacctcaa aaaaatagat ggtgataatt ttaaattcat ctatttacca
     9541 gaaaaagcag ctgatccgat taactttatt gagattttgc cagatgtaaa aacggggaaa
     9601 ccttacccgt tggcgatgtt ccagaagttt attattggga atttatatgg atggcgaaag
     9661 aaaacagatc attccttgag acgttttaga aaagctatga tttctgttgc tcgtaaaaac
     9721 ggtaaaacaa ttctgatagc tggtatcttg ctttacgaat ttttgtttgg ccataaccca
     9781 tctatgagca gacaattgtt ttgtactgca aatgatagaa cgcaggctaa aatcgcttgg
     9841 gatatggcaa agaagcagtt atcatctctc agagcgaagg acgctgatgt cagaaaggct
     9901 acaaaaattg tccgtgatga gcttaaaaac ttacatgacg aatcatatat cagagcactt
     9961 agtcgagata ctggggctgt agatggattt gagccttatg ttggcgtttt ggatgaattt
    10021 gcagcatcga agacgaatga aatgttagaa cttttagaat ctggtcaagg tcagcttgat
    10081 aacccgttta tcttgattat ttcgacggca gggatggatt tgaatgtccc tatgcacaca
    10141 attgagtatc catacatcac taaaatacta gacggagaaa ttgtagatga tggctatttt
    10201 ggttatgtcg cagaacagga caacgaagag gaaattaaag acgaatcaaa ctggataaaa
    10261 tcaaatccaa tccttgaggt tgaagcttta catgataagc taatggatta cctgagaacg
    10321 cgccgtagag tgtctcttga aactggtgaa atcaataaag tattaatcaa aaactttaac
    10381 atgtggcgtc agtctagcga ggaatcttac atcgataaaa cgacttggga acttgctcgg
    10441 attgataagc cagatactaa taagcggaga gtttggttag gtgttgacgt tggtcgtgtt
    10501 agcgacttat ttgctatcac accagttgtt atgatggatg atttttggta tatcgacagc
    10561 ttttcttttg tggctaccaa atatggttta actgctaaag aaaagcggga tggcgtatct
    10621 tatagcaatc ttgaacgcca aggttattgc gagataacca ccctagaaag tggtgtcatc
    10681 gatgatgaac gtgtactcga aaaaattgaa gagatggtct actcaaacga ctgggaaatt
    10741 aatggaattt gttttgaccc ttatcaattt ggaacattac ttacaatgat tgaaaaaagg
    10801 catccagagt ggcctctgat tgaggtttcg cagacgacaa tggttttgaa catgccgaca
    10861 aaacaatttc gtgacgacct caaaaaaggc aaaataaagc actctggtaa ccctttgcta
    10921 accatggcta ttaacaatgc ttatattaaa actgataata atggtatgag gatcgataag
    10981 aataagaata gcaataagat tgacccgctt gatgctgctc ttgatggcta cgctgtttgt
    11041 tacttagaac cgttcgatgg ttctggctac tggacaaatg aaaaaataat aggaggagaa
    11101 tcgctgtttt gattgatttt attttaaaaa acatacacac attaatcttg ttagctggac
    11161 taggtttatt gatgtatggg ttgtttttgt ttggtgataa agtcggtttt attgccagtg
    11221 gtcttatttt aattgtttta gctatctatg tagatagcgt aggaggaaaa cgtgaataaa
    11281 cgcattaaga aaaaacgaaa attggaaaca gctattgtgt tgctaattaa agaagtcgcc
    11341 gaattacgat ccatcgtgtc agcaaatgcc aaagctacaa ataacgagct tgcagcagtt
    11401 aaatcagcga tactagacaa tcaagtagct atcaagtcaa ttggtgatga ggttggtcac
    11461 atcaagcaaa attataagcg caagtggcgg aaatagatgt taatggttta gaaaggaggt
    11521 gagaaatcga tgagtttttt ccgacctttg ggcagttcaa aggtgtccta cgatgactat
    11581 atatcatctg ttttagctgg tgatgtctct caaaaatact taggggtgtc ggctttgaag
    11641 aatagcgata ttttaacagc aacgtctatt atagctgggg atattgctag gttcccgctt
    11701 gttaaaaagg atgttaatgg ggacattatc catgatgagg atattaatta tcttttaaat
    11761 gttaaatcta caaaaaatgc gagcgccagg acatggaaat ttgctatggc agtaaatgcc
    11821 attttgactg gtaattcttt ttcgcgtatt ttgagagatc caaagactaa tcaagctttg
    11881 caattccaat tttacaggcc atcagaaaca acagtagagg aaacggatag tcacgaaatc
    11941 atctacactt ttactgatac gttaacagca aaacaggtta aatgctttgc tcatgatgtt
    12001 gtacactgga agttttttag tcacgacaca atccttggca ggtctccgct actgtcttta
    12061 ggagatgaga ttgatttgca aacaggtggt atcaatacct taattaaatt ctttaaagat
    12121 ggattttcta gcggtatctt aaccatgaaa ggcgctcaat taagcggaga cgcacggcag
    12181 cgagcacgtc aagagtttga gaaaatgcgc gagggttcgg tcggtggcag tccattggtg
    12241 tttgatagta ccatggaata cacgccgctt gaaatcgata ctaacgtatt gcaattaatt
    12301 acaagtaaca atttttcaac ggcgcaaatc gcaaaagctt tgcgggttcc cagctacaag
    12361 cttggtgtta atagccctaa tcaatccgta gctcaactga tggaggacta tgtcacaaac
    12421 gacttgcctt tttattttga cgcgattaca agcgaattag gtcttaaaac gttaaatgat
    12481 aaagatagac gtctctatcg tattgaattt gatacacgta gcgttacagg ccgtaatgtt
    12541 gatgagattg tcaaattggt taacaatcaa atactgacac ctaaccaagg ccttattgag
    12601 ttaggtaagc agaaatctac agatcctaat atggataggt accaatcgag tctaaactat
    12661 gtctttttgg ataaaaaaga agaatatcag gacaaggttg gtatcaaagg gaaaggaggt
    12721 gaggtaaatg ccaaagagga taaatcttaa aggcccactt attgcaaata attcgcaaga
    12781 agtttatgac tactatggaa tggaagcgac tagtgccaaa agcattatta accaattgcc
    12841 agaagatagc agtgatatta ttttggaagt taattcaaac ggtggtcttg ttacagtagg
    12901 gagtgaagtc tataccgctt tgcgaaatta caaaggaaaa gtaactgcag aaattactgg
    12961 catggctgca agtgcagcat ccgttgcagt tatgggagca gataaagttg tcatgagtcc
    13021 aacagcacag atgatggtac acaaggcttt gtttaatttg gtgtgtggta atagcgatga
    13081 cctagacaaa gcttctaacg ccttaaaatc aagtgataaa gctatcgtga atgcctatgt
    13141 cgcaaagaca gggttatcgg aagatgaaat catgaattta atgcggaatg aaacgtttat
    13201 gtctgctcaa gacgctgttg aaaaaggttt tgctgatgaa gtgatgtctt ttgaagcggt
    13261 ggcgagtatc gataaccaaa tgttgccaca agctgttatt gacgactatt acgcaaacag
    13321 aagtaagcgc aagcaagaga ttagcaacat gttgctagaa attgaaaaag aagaaatttt
    13381 acaagggcta taagctcttt ttttattgga ggaatttatg ttcgaagaaa aaatcaaaga
    13441 aattaaagcg actatcgcta gtttaaacca agcgattgct actaaaacag ccgaagtaaa
    13501 aaatgctttg gaatcagatg accttgaaac tgctcgctca attaaagcag aagttgaaca
    13561 agcaaaagcg gacctagtag aagcagaaaa cgacttgaag ttatatgaag ctagcattga
    13621 aaaaggcggt gcagaaaata ctggaggaaa agaagtgcca caagaaacta aaacataccg
    13681 cgaaagcgtt aacgaattta ttcgttcaaa aggaacagta actaacgaag ctttgcgttt
    13741 cgaagggaaa gacgaggttc ttatcccact taaccaaacg actcctatag atcctaaaac
    13801 ggacggtgtt aagaaaacag atgtaaaacc tgtctctagt gaagaaattc tttacacacc
    13861 agctcgtgaa gttaagacag ttgttgattt gaaacaattc actagcatcc acccagctaa
    13921 aaaagcgtca gggaaatggc cagtattgca atgtgcgact gaaaaaatgg ttagcgttga
    13981 agagctcgaa aaaaatccaa agcttgctaa accaaaattt aaagacgtag agtggaaagt
    14041 cgagacttat cgtggagcca tcccattatc tcaagagtca attgacgatg cagatgttga
    14101 tttagttgga attgttgctg aaacaatcgg tcaaatgaaa gttaatacaa ctaatgatgc
    14161 aattgctaaa gtcctaaaaa catttgagcc taaagctgat gtcaaaacat tagatgaaat
    14221 caaaaaaatc ctaaatgtag atttagatcc agcttacaat gtgtcattta tcgtatctca
    14281 aagcttttac cagtccatgg atactttaaa agacgaaaac ggtcgttatc tactccaaga
    14341 ctcaatcact tctgttactg ggaaagtatt ccttggaaaa cctatttttg tactagccga
    14401 tgaggtttta ggtaaaaata aagcatttgt cggagatttc aaacgtggtg tgttattcgc
    14461 cgaccgcaaa gacttaggtt tgcgttgggt cgataatgac atttacggtc aatatttgca
    14521 agctgtgtta cgtttcggtg ttgctaaggt agatgacaaa gctggctact atgtgacatt
    14581 tacgccagaa caattgccct cgtaaggcca ctgaagaagt ggcgaaacca acaagcaaaa
    14641 gcactgtaga agaaatcaaa cgctatttaa caagcaaggg aattgacttt agtggtaaga
    14701 cattgaaatc agatttactt gcactagcag gcgttgaaga ggtatagcca tggctgtatc
    14761 gaaagagtta ttagacagtg taaagctcta ttgtaaaatt gactttgatt ttgaggatga
    14821 catcattaaa gaaatgattg aatctgctca ggaacaaatt tgttttgcga tagaagaagg
    14881 ctcaaccgca gacacgttta aggaaagcgc taaatttgct ttagcagtca aaaaacaagt
    14941 caaggaagag tatgaccatc gcggtttgtc tgcggatagt tttcgctatc cgttggcaaa
    15001 tggcgtttta aatatcattc atcaactacg attgcggggt gatgattcat gattacacgc
    15061 aagatgaacg tcaggataac gatttttagc caatcaggtg ggcaaaatga agatggtgaa
    15121 gtagtatctg ctatcagaaa agacttatat acctgttggg cagaagtatt gaagacgcag
    15181 ttaagagact ttaattacca atcaaagttt caaaacgcta gcaatctgcc gacaaataag
    15241 gatacaaagg tttttttaat taggtataac cctcaattat ctatagataa cacgatgttt
    15301 gttgaattca acaagcgcat ctataagata gataaaatcg aatctgacga atctggcaaa
    15361 gatattacta tgattagtgg agtaagcatg tcatgacaag aggtttagat gaaatattag
    15421 ctaatctgac aaagcttgaa gtaaaagccc caaaaaccgc aaaagttgcc gtgactaagg
    15481 ttgcgaaaga atttgagaag accctcaaat caaatacccc tgtttatgag attgaaacag
    15541 atgagcgact acaggaagat accgttatca gcggtttcaa aggttctaat gtcggcattg
    15601 tttcaaaaga aatcggttat ggtaaagcga caggttggcg tgctcactat cctaacgatg
    15661 ggacaattta tcaacgtgga caggatttca aggaaagaac aatcaatcag atgacaccga
    15721 gagccagaca aatttatgct gaaaaagtta aggagggact aggactttga ttgctgaaac
    15781 agcagcttat aaattgttaa gtaacgataa gacgctaaat gagctgttgg ataggctcag
    15841 aggcggtact tttaaaaatg gatttaaaca agggattttt acttatgata tcccggataa
    15901 tcctgttaac ttacgaaaag tagaattggc accatttatg cgaattaaca caacgttaga
    15961 tggtcctttt gattatgctg atgacgagat actttgcaat gagcaacgta taactattaa
    16021 tttttggtgc aaaacagctt cagaagctga ccaaattaac aaatgtatag ataacatttt
    16081 aaaacaaggc ggttttgaaa gatataccgc gaatgaaaaa ccaagatata aagatagaga
    16141 tattgactta ctcatgaatg taaggaaata ccgctatttt gatttttatt aaaagaaaga
    16201 ggaaaaaatg ggaaaagtaa aatttggact acgtgatttt tactatggag tactagacgg
    16261 aaatgacaag gttacaaaag cagagagtat aaagcatttg ccgggaatga aatccgcaaa
    16321 acttgatatt accaatgaat tggtcactgt gccagcagac gatggacctt acgttgtatt
    16381 atcaggtgga atcagcgaga caaaacttga aatcgagttg ttagatttaa catcagatgc
    16441 acgcaaagat ttctttggaa tcacagtaga aaaaggtgtt gagaagtaca acaaaaacct
    16501 aacgccaaat gacattgttt gtttatttag gactagcgat gaaaacggta aggcgatttg
    16561 gattgcgttg cttaaaggga aatttaatct tccgggaatg gaggcacaaa caaaagaagg
    16621 cgcacctgat ccgaaaccag atactacaac gggtaacttt gttgcccgcg gaccaaaaga
    16681 ggaagtgctt ttggtcgggc gcgagggtaa tagcggtttt aatttgaatg aatttttaaa
    16741 atgtgtattt gccggctgcg gtgatgcaga aattaaaaaa ataccaattg cggaacaagc
    16801 aagtacggtt atgtaatttt taaggtcgca tttaagctgc gaccttttat ttttgacaag
    16861 gagtagatat gtacgaaatt accttaaaaa aaggcggtgt tgataaggaa tttaaaaaag
    16921 actttatcaa tgtcgaagat aatttgttgg ctgttgagca tcaagttaga caaagcgctg
    16981 tgtttagcga tgagaaaaga cgtttagatt ctaaagcgca tagaaaactt aacgaatctt
    17041 acttgcaaat gtttgttgat atgtacgcag gtcagtttac cgttgatgat ttaaagcaat
    17101 ctgatatgac cgttttaaat acacttaatg acctgtacat tgcagcactc ggaggagagc
    17161 aggaggaaga cgaaagcgaa aaaaaggaac aataacccca caagaggcta aagaaaactt
    17221 actcttgtgg atacagagcc ttttgaaaaa cggttatacc attttagata ttaaaaaaat
    17281 gcgcttatca gacatcgaat tgatggtaca agcactagaa attgaaactg tcgaaaaaga
    17341 agaagtgatt gaaacgacct tggataaggc attcccattc cttttcggct agaaaggaga
    17401 ctaaatgggg aatataggtg atttagtagc aacagctaca ttagacatat caccctttat
    17461 gtcaaacaca agaaacctaa aaacctccat gaaaggctta gataactctt taaaagcagt
    17521 tgaaaagagc tttcaagggc atggcgggcg gattaaaggt cttaaagcgg tctatgctga
    17581 aacaggtagt gcattaaaag gttaccaaga attactaaaa actcagtcac aaaaatatag
    17641 cgacctaaaa aaagagatag gtgatgttaa taacgctact gctgagcaaa aacaaaaatt
    17701 aattggcgct aaatcagtca tgctagaaac agcagcgcaa gtggcggaat tgcaaaacag
    17761 attacgagct ttagccactg aaactagcgt ctttacaaga tttggtaaag ctgctgaaag
    17821 aattggcggg aagatgaagt cattcggcga ttctgtcgcc ggtgtaggtg ctgcatttac
    17881 aagaggagtt acagctccga ttgttgcagg agctggttat gctattaaag cggctgtcga
    17941 ttatgaatct gcttttgccg gtgtcaagaa aaccgtggat gaaacggcga cggtatccta
    18001 tgctaagttg tcgcaaggca ttagacaaat ggccaaagag ttgccagcca gtgctgttga
    18061 aatcgctcac gttgcagaag cagcaggtca attaggagtt aagacaggag atattcttag
    18121 tttctctcgt acaatgattg atttaggaga atctaccaat ttatccgcag aagaagcggc
    18181 gacgtctatt gctaaaattg caaatattac aggtctagca tcatctgagt attcgcgttt
    18241 tggaagtgct gtcgttgcgt tagggaataa ctttgcgaca actgaaaaag acattgttgc
    18301 aatgaccaat cgtatagcag catctggtaa gcttgcggga ttaactaatc aggagatgtt
    18361 agctttagct acagcaatgt caagcgttgg tatagaggcg gaggctggtg gtacagcaat
    18421 gactcaatca ttatcagcta ttgaacgtgc agtcgcatct ggaggcgata atttaaataa
    18481 atttgctcag atagctaaca tgtcctcagc cgattttgct agagcgtgga aagaaaagcc
    18541 aattgtcgca ttgcaagagt ttattaaagg gcttggtcaa cttgataaac aaggcgaaag
    18601 tgccacaaaa gtacttgatg agttaggatt aagcggtatt cgccagtcta acatgttgaa
    18661 atcattaggt ttagcatctg aaacattagg caaggcactt ggaatttcca ataaggcttg
    18721 gaaagaaaac acggcgttga ctgacgaagc taacaaacgt tacgagacaa cagagtctaa
    18781 gctgaaaatg cttaaaaacg aagtcaatga tgtagccata gaatttggtg gtcctttggt
    18841 tgacgctctg agaaacgggc tcgaagcagg gaagccaatc atccaaatgg cggctgactt
    18901 agctaaacaa tttaactcgc tcgacaaaga gcaacaacag caaattatca agtggggact
    18961 tattgcagcc gccgctgggc cggctttatc tattttgggt aagggtattg gtgttatcgg
    19021 cggaaccatt caagctattg gcaagatgag caaagggatt ggtgctttat ctggttggct
    19081 acgcacgttt aaagccggtg cagtagcagc aagtgttgga gctgaagctg ccacagcttc
    19141 tatgggcagt ttgactggcg cggttgcctt attaagcaac ccggtaacgt ggggtgtttt
    19201 gctaggtggc gcagttgtta ttggtattgg tttaattgct gatagcatgt ataaagccca
    19261 aaaacgcacg gaagagtggg gaaccgctgt ttccgcgaca gaagcaactg cactaagtaa
    19321 ctttaagaaa aaagttgacg aaactaacac ttctttgcaa atgttcgagg caggcgcagg
    19381 tagcgttaag aaagtgactg aagctttcga tgatttggtc ggaagtattg aaaagttagc
    19441 tcaatcaaaa ttagataaga atataaactt agctaaaaaa ttaggattgt cagaagaaac
    19501 cataaatgct ttgaaatcca aaactgaatc agtggttaac aatgttaaaa gcatgaacac
    19561 ccagattaaa gcaatcatgg agaagcacaa tggtgacatg agccagttgt caagcgctga
    19621 gaaagaactt gttttgcgaa atcagagaga aatgattatt gctcaacttg atttaattaa
    19681 gttttctgca tcagaaaaga aagctttaac agcagctttg aataacgagt tagatgcgat
    19741 aaatgcaagg cagttggaaa aagtatctga aaataccgtc aagatgcttg ataaagaaaa
    19801 ctctgcatat aaaacaaaaa aagcagagtt aaaagagatt ttgaagcaat ttggcagcga
    19861 cactagtaaa ttgagtgctg aagagttggc tgctagacag gaagttttga atagacttac
    19921 agaacttaat atgcagcaca acctaaaaac caaagctttg aatgatcagt atcttgctat
    19981 ccagagggag cgagtccaac ggttaaaaga atctggtaaa agtcaagagg aaatccaccg
    20041 cggcataagt caaatggcgt cagatatggc tcagaagctt ggcattagct atgatgacgc
    20101 ttatcgcaaa atggcttact ataccgaaaa atccggtgaa acgttgaaag ttttatcacg
    20161 taataccgct aatgctactg cagaggtagc agcagcaaat gctcaatggg acagtttgtt
    20221 tacgagtgat aatccacaac aaagtttaaa cgaattgtta tcaacggcag aaggttggaa
    20281 tagctttgaa atcatggtta agaacgctga tgttgaaccg acggggagag ccgcacttgc
    20341 tgaaatgcta gtagctggtg gccaatggca aaacatgacg ttagaacaga aaaaattggt
    20401 tgtggatggt caacaggcta tgattgaaat ctttgatagc aaagaattac tagcacaatg
    20461 gcaagcgttg acaccggaag aaaaagttct gttagcgaaa aacttaacac aagaaccaac
    20521 tatgtccgct caacaagctc ttgatagcgt taaacaaaca gtacctgctg atgtgaatgc
    20581 tacggataaa acagcaggtg atactcagtc ggcgcaaagt aagattgata atgtcaagca
    20641 gaaagcgcca gccgatgtga aggcatcgga taagactaga ccagatgttg caagcgccaa
    20701 tagggcagtc aatagtccta aacaaaatag tccagctgtt attagagcac aagataacgc
    20761 aagcggcgtt gcagaaaatg ttatatggtc actggctaga atcccaagaa gtgttacaac
    20821 aaccattaca acgtttgtcc gtaagatttt cggacacgaa aaagggactg atttccaccc
    20881 tggcgggtta gctgtggtca atgaccaaaa aggggcgcta tatagagagt tagttacctt
    20941 accgactgga gaatcattta tcccaactgg tcgaaacgtt atcctccctc taccgagagg
    21001 gtcaaaagtt ttaaaggcca gccgaacaaa acagttattc ccgcactatg caaatgaaat
    21061 aggttttgat gatacaaaaa tcgctagctt aacaactcgt cttaaatctg tgcaagataa
    21121 aggaactgta gtcgttaacg ctgatccaca acttaccgag ttgattaagc tgcttaaaga
    21181 cagagatgac agaaatgtca caaacaacta tacactaaac gctactaata gcagtagttc
    21241 agaagatatt tttagtcaaa aaaacatgag acggctactc agagaattag cttactacac
    21301 aaaaggtgaa gaagggaggt tagcttagtg agatacattg agtttaacgg cactaaaagc
    21361 gatagtctag gtttgttgct agaacgcgag cggtctatta agtcaacgag taacgatgtc
    21421 aatttaatag aagtcgatgg tcgtgacggt gtacttatta aagacaacga acgcttaaaa
    21481 gcggttgagc aagactttcc tttttcgctg gttggtgacg tggctgtcaa tcagcaaaaa
    21541 ataagcgagt ggttgcacgt caaaggttgg catgacttag ctttgtcttg ggacaaggac
    21601 tatatctatc gggctagtgt tgtcaatctt tttgagatag acgagatact taagcaattt
    21661 ggtagattaa aggttaattt cttaatccac cctatcaaat atttaaaaac aggtaagcaa
    21721 gaggtgcctc ttgttaatgg cgatacccta caaaatcccg gcaatgtaca ggctaaacct
    21781 atcttaaaaa tcaaaggcac aggcgatggt gttttaacca ttaacggttt tgagacagga
    21841 cttgaaaatg tgcaaaggga actcgtcata gacatggaaa ggcaccttgt ctataaagac
    21901 gtcctatctg cttgggataa tatcgttcgg acaaagcgtc accgcatgcc actatttgat
    21961 gttggtcaaa acacaatctc gtggaccggt aactttacga ttaccgcagt accaaactgg
    22021 ggggtcaaag tatgatacca gttttatacg aggctaaaga gactaggttt aggacttttg
    22081 gtcttggaga gattgcagat gcttacgagg ttaaagtcac tcgtgagcgt aatggtaatt
    22141 actcgctata tatcaaatat ccactagatg gtgtctttgc ctcagttttt aaagaggaga
    22201 tgaagattaa gtctgatgca ggtcgtagga ccaaatggca gacatttgag attaatcgag
    22261 tgctacggaa tagtaaagac cacattgaga tttttgcgcg tcatatctct atgcgcacac
    22321 aggatattgc tttaaagcca tctgtgagcg gatctagcgt cggagctgaa tcagctttgg
    22381 aaatctggaa ggaaaacctt gtcggtgatg atacttttga tgttaaaagc gacatcctaa
    22441 cgctcgggag ctttagctgg gaagctgata aaatcggcaa tgcccgtggt gctctaggag
    22501 gtgtcgctgg ctctatccta gatgtttacg gtggcgagta cgagtttgac aaccgtacaa
    22561 tcatcttacg caagcaaatg gggcgtaaag ctcccacggt attggagtat ggccgtaata
    22621 tcgtcagcgt agaggaggag cgcttgctag atggcaatta cacctctatc tatccttacg
    22681 taagatatac gccacaacca aaaccgcaag aggaagcccc tggtaagccg catgtaggcg
    22741 agcataaaca acccgaagaa cagctggtga cattgcctga atttatccta gatggtcaat
    22801 atctcagctt atatgctcag cgcagaatcc aaatggttga tttatcaagt cattttaacg
    22861 atgacaaaaa taaaaaagag ccaacagtcg aagaaattag aaagttggct cagaagtacc
    22921 ttaaggataa taacgtaggc gcccctaaag tcagcattga ggttgattat atcgacttgt
    22981 cacaaacact tgactaccaa gattttaggg tcatggagga ggttgagctt tgcgacattg
    23041 taccgcttta ttatccaaag tttggcatca cgactgagtc tgaaaaagta gttgagattg
    23101 tctatgacgt ctatacagat agcaatcaca caatcaaatt aggtacgatt ggtcaatcaa
    23161 tctctaaaag tttgactggt ggtgtttttg aacgtattaa tgcgttggaa aataatcaaa
    23221 aggtaattac taacaaccaa aaacaatttg aactcaatct gcctaaatac ctcaatgaca
    23281 tcaatggtaa acgcgtttgg tacgaaaaac cagatgacaa tattgagcac aagataggtg
    23341 attactggtt cgaaaaaaat ggtaagtatc agcgcacttg gatttgggat ggccatcaat
    23401 gggtcaaggt actagataca gaggatttaa accctaacca acgggccttt gacgaggcaa
    23461 tggctgaaat cgaaaaagcc aaaaaagcgc a
//
LOCUS       DAUCWU010000017        22454 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUCWU010000017 DAUCWU010000000
VERSION     DAUCWU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.017"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(89..394)
                     /locus_tag="VGY24_000671"
     CDS             complement(89..394)
                     /locus_tag="VGY24_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ4283674.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(406..825)
                     /gene="ruvX"
                     /locus_tag="VGY24_000672"
     CDS             complement(406..825)
                     /gene="ruvX"
                     /locus_tag="VGY24_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ4283675.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(822..1091)
                     /locus_tag="VGY24_000673"
     CDS             complement(822..1091)
                     /locus_tag="VGY24_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ4283676.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1206..1604)
                     /gene="spx"
                     /locus_tag="VGY24_000674"
     CDS             complement(1206..1604)
                     /gene="spx"
                     /locus_tag="VGY24_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ4283677.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(1895..3031)
                     /gene="recA"
                     /locus_tag="VGY24_000675"
     CDS             complement(1895..3031)
                     /gene="recA"
                     /locus_tag="VGY24_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ4283678.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            complement(3120..4391)
                     /locus_tag="VGY24_000676"
     CDS             complement(3120..4391)
                     /locus_tag="VGY24_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ4283679.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSNLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLAHETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4460..5020)
                     /locus_tag="VGY24_000677"
     CDS             complement(4460..5020)
                     /locus_tag="VGY24_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ4283680.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLDDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQKEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5030..5626)
                     /gene="ruvA"
                     /locus_tag="VGY24_000678"
     CDS             complement(5030..5626)
                     /gene="ruvA"
                     /locus_tag="VGY24_000678"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ4283681.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGHTKARSNKAGNTQLDEAIEAL
                     LALGYKAKELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(5628..6848)
                     /locus_tag="VGY24_000679"
     CDS             complement(5628..6848)
                     /locus_tag="VGY24_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4283682.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFQDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(6859..8841)
                     /gene="mutL"
                     /locus_tag="VGY24_000680"
     CDS             complement(6859..8841)
                     /gene="mutL"
                     /locus_tag="VGY24_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ4283683.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTSDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKVELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLTRLE
                     NEGQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(8970..11525)
                     /gene="mutS"
                     /locus_tag="VGY24_000681"
     CDS             complement(8970..11525)
                     /gene="mutS"
                     /locus_tag="VGY24_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ4283684.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCIDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRNGFDERLDHYRKVMREGTGWIADIEAKERQESGINNL
                     KIDYNKKDGYYFHVTTSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPEALLSRADEVLTRLEAQAQSAEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(11512..11864)
                     /locus_tag="VGY24_000682"
                     /pseudo
     CDS             complement(11512..11864)
                     /locus_tag="VGY24_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            complement(11861..12298)
                     /gene="argR"
                     /locus_tag="VGY24_000683"
     CDS             complement(11861..12298)
                     /gene="argR"
                     /locus_tag="VGY24_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4283685.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            12589..14280
                     /locus_tag="VGY24_000684"
     CDS             12589..14280
                     /locus_tag="VGY24_000684"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ4283686.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYVQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLSDKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDNNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            14368..14676
                     /locus_tag="VGY24_000685"
     CDS             14368..14676
                     /locus_tag="VGY24_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ4283687.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(14703..15575)
                     /locus_tag="VGY24_000686"
     CDS             complement(14703..15575)
                     /locus_tag="VGY24_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4283688.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(15618..16496)
                     /locus_tag="VGY24_000687"
     CDS             complement(15618..16496)
                     /locus_tag="VGY24_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4283689.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            complement(16516..17457)
                     /locus_tag="VGY24_000688"
     CDS             complement(16516..17457)
                     /locus_tag="VGY24_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4283690.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIIPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            complement(17450..19198)
                     /gene="aspS"
                     /locus_tag="VGY24_000689"
     CDS             complement(17450..19198)
                     /gene="aspS"
                     /locus_tag="VGY24_000689"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ4283691.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANAQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            complement(19536..20816)
                     /locus_tag="VGY24_000690"
     CDS             complement(19536..20816)
                     /locus_tag="VGY24_000690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ4283692.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQELEVSFEDMMTNFENISEQLLS"
     gene            21036..21218
                     /gene="rpmF"
                     /locus_tag="VGY24_000691"
     CDS             21036..21218
                     /gene="rpmF"
                     /locus_tag="VGY24_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ4283693.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            21234..21383
                     /gene="rpmG"
                     /locus_tag="VGY24_000692"
     CDS             21234..21383
                     /gene="rpmG"
                     /locus_tag="VGY24_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ4283694.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            21676..22290
                     /locus_tag="VGY24_000693"
     CDS             21676..22290
                     /locus_tag="VGY24_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ4283695.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGEILEKYSRWFIAVVYLGLGIYILIENNSFDMLWAVLG"
     gene            22302..>22454
                     /gene="cadX"
                     /locus_tag="VGY24_000694"
     CDS             22302..>22454
                     /gene="cadX"
                     /locus_tag="VGY24_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ4283696.1"
                     /translation="MKKDSICQVNVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYL"
BASE COUNT         6960 a         4883 c         3824 g         6787 t
ORIGIN      
        1 tcattatcac ctcctgctta tttattcgaa aataacatta aaaaaaatag ccctcacggc
       61 tactttttaa catagtgtca ctaacaattt agttctcatc tagaaaacta ttaaatacct
      121 cttcaatcat atcccattca gcatctgaat cctcaggaat cggttgtaaa tccccttcag
      181 taccatcttc attttcagta aatgaatagg cttggatttc aatttcacca gactcatctt
      241 cttcagatcc cgctggaacc aagagaacat aatttttacc aaactcttca cgaccatcaa
      301 ttgttaataa aatttcaaac aaggtttcat ttccttgttc atccacaagt gtgataactt
      361 cgtgttggtg gtcattttca tgattatgtg tcatttttta tctccttaaa aattccgatt
      421 taaatagttc tgtaaaatga gttgtgcagc taatttatca atgacttttt ttcgcttacc
      481 acgactaatg tcagcttgct caataagcat acgctctgcc tcaacagtcg tcaatcgttc
      541 atcttgataa tggactggaa gtccaaaaag gtgctctatg tggtttccat aagttatact
      601 tgcatcaaca cgtggaccat tggtgttatt catatttttt ggcaatccaa taacaaactg
      661 ttcaacttgg tactgcttaa caagctcttc taaacgtgta aagccaaatt ctgctttttc
      721 ctcatcaatc ttgataattt caagtccttg ggctgtaaag ccaagaggat cactaatagc
      781 tacaccaacc gttttcgagc caacatcaag tcccattatt ctcatttaac atcaattcca
      841 tttccttgta aatagtagcg aacaagttct tctacaattt catcacgttc atatttacga
      901 atctgatttc gagcatcatt ataacgagga acataagctg ggtccccact caagacataa
      961 ccaacaattt gattaattgg attataaccc ttttcatcaa gtgaatgata gactgctgtt
     1021 aatgtttcac taatttgtcg tttgtcgcca tcatccaact taaagcggac tgtttcatct
     1081 gtaaatccca tacttacacc ttctttctaa aattaagtgc tatgctttat tatagcttat
     1141 ttctattaaa taaacaagaa tttcaagcga agaaggtatt taaaaaaaaa gctctacaag
     1201 agcttttaga gtgcagcacg taatcgtgct tcagtatttt caatattacg aatagagcga
     1261 ggtaaaaaag cacgaatatc gtcttctttg tagcctactt gcagacgttt atcatcaatc
     1321 aaaatgggac ttttaaggat gcgcgggtta tcttgaatca aatcaataac ctcactaaca
     1381 cttaattctt caatatcgca atcgagagct ttggcataac ggtttttaga tgaaacaatg
     1441 ctctccactc cattttcggt ttttgataaa attgctaaaa tttcttcttt agttagcggt
     1501 tcttttccta agttctgttc tttataagca agcttatggg catttagcca agttttggct
     1561 ttcttgcagc tcgtacaact tgaaatcgtg taaattttaa tcatgttgct gactcacttt
     1621 ctatttcgtg atagctttat tgtagcacag tttcttatct ataaatatag gtctttggac
     1681 ctattttttt aattttaaag atattatctt agtaaaataa ggtttttcac ctgtattccc
     1741 ttgaattatt agttatttta attttatatc agttaaccta agttctgctc tctcacatct
     1801 ttttaaatga tggtttctat tcacacacac acctatgaac agtagtccta ctaaccttta
     1861 aaaatacatt ttaaaaagcc ctgaagggct ctttttaatc ttcaatttca ataccattat
     1921 ctaaatctaa agcaagatca tcggtttctt ctgatgctac tgccatagca gattcttctt
     1981 cgctttcttc aagcaaacca aatttaacac gtactttacg gtcgatttca tcaaacaatt
     2041 ctggatgatc ggccaaataa cgcttagcat tttcagaacc ttggccaatc ttctcaccat
     2101 tataagagaa ccaagcacct gctttttgga taatgtccaa atcagaagca attttcacaa
     2161 gctcccctgt acgagaaata ccttccccat acatgatttc aacttctgct accttaaatg
     2221 gcggagcgac cttgttttta acaaccttga ttttggtctc cttaccaatg ctgctatctt
     2281 tttggtcacc agttccttta atttgagttg ttccacgcac atccagccga acagaagcat
     2341 agaatttcaa agctcgacca cctggtgttg tttcaggatt tccaaacatc acaccaactt
     2401 tttcacgcaa ttggttaata aagatagcga tagtttttgt tttattaata gaagctgata
     2461 atttacgcat ggcctgactc atcatacgtg cttgcaatcc gacatggcta tcgccaatat
     2521 caccatcaat ttcagcacgt ggcactaaag ctgctactga atcgacaaca accagatcaa
     2581 ccgcaccaga atcaatcaat ttacctgcaa tttcaagtcc ttgttctcca gaatctggtt
     2641 gagacaagag gagttcatca atattaaccc caagcgcagc agcataagct ggatcaagcg
     2701 catgctcggc atcgataaag gctgcgattc caccttcttt ttgagcttgt gctacagcat
     2761 gtaaagccac agtcgtttta ccggaagact ctggaccata gatttcgatg atacgtcctt
     2821 taggataacc accagctcca agcgcaatat caagagctag acttcctgaa ctcataacct
     2881 gaactttttg ctctgcacgt tctcctaatc gcatcactgc gcccttacca aaatcttttt
     2941 caatattttt caaagcatca tcaagggctt tgcgacgttc gtcaccaaac ttcttagtga
     3001 tttcttcatt cttttttaat ttttttgcca atactttctc ctctttttta acaaaccatc
     3061 gtcagcagga ttaatctaca taatcgtaaa ccatatccta ctatagctac taaataacat
     3121 tatagcaaat tctcaagttt taataaagtt ttacggacca tattaaaggc atgtaaagta
     3181 gcgatatagc gcacatccaa acgacttcgc ccgctaatca aaacctttat tgattctacc
     3241 ttattttgag tggcaagacc aatgaaaact gtacctgcag gctgttcctc caacatatct
     3301 ggtccggcaa cacctgttaa acttacccca atatcagcac cagtcaataa ccttgcttgc
     3361 tccgccatcc cctcggccgt ataagcacta acaacgccat gcgatttcaa ctcctctaaa
     3421 ggaaggccta gcattttcgc tttttcttcc atgctataag tcacaaagcc tccattgaat
     3481 acttgagaag ctcctggaaa actcgccaaa gttgactgaa ataatcccgc ggttagactt
     3541 tctgctgctg taattgtctt atcatatttt actaagagct caaatgtctc atgcgctaag
     3601 gaattatcct ccccatagcc ataaaagaca tcagctaagg gttgaccttc aagagttttt
     3661 cgggatagta gctgcgcttc tagctggcct aactttttat ctgccagagc ttggttttca
     3721 gtttttgttg ataagcgaag agtcacttcg ccagtcttag catatggagc aatggttgga
     3781 tcagtttgat tctcaataaa atctgacaag actgttacca actgactttc cccaatacca
     3841 aaaaagcgta gtacctttga atacaatgta ctgtattgtt ttgacagaag tggtaccaat
     3901 tcttcattta ccatcggctt caattcactt ggaggacccg gtaagacaac ataactaata
     3961 ccatcgactg tgatgaaccc accaaccgca agaccagttt tatttggcaa agggattgac
     4021 ccttcaatca cttgtgcctg tcgctcatta tttggtgtcc gtgatgaagg cttgcgctta
     4081 gcaaaaaagt catctagttt ctgacaagct tgctcatcat aaaccaagtc tttcctaagg
     4141 tactttgcta aagtttgttt ggttaaatca tctttcgttg gaccaaggcc accacataaa
     4201 attaccaagt tactacgctg actagcagtt gtaatcactg aaagtaaacg ctcctcgttg
     4261 tccccaacag ccgtttgaaa atagacatca atacctagct ctgccatttt ttccgacaga
     4321 aattgagcat tggtattcac aatttgacca gtcaaaattt cggtacctac tgcaatcagt
     4381 tcagctttca tttccccacc tacttatcta ttcgtaatct gatctcattt tatcaaaaaa
     4441 tcttccaatt cacgaagaat tagaagattc tattgaaaac acaagcctct tcatggtcat
     4501 ttaccaaacc cgatgcttgc ataaaagaat aaaccgttgt aggccctaaa aatttaaatc
     4561 ctcttttctt taaatcctta gctaagcgaa tagataattc tgtttgagca ggaacaagat
     4621 tctcttgatt gacaaggttg ttaattggct ttcccccaac aaaattccag agataagttg
     4681 aaaaactacc aaactctttt tgaattttct gtactgcgat agcgttgttg acagttgctg
     4741 ctagctttag cttgtggcgg ataatggatg gattctctaa agcatcagcc atttcctccg
     4801 aggtaaaggt tgcaaccgat gcaatgtcgt aatgatgaaa aacagttctg aaagcttggc
     4861 gttttttcaa aacagtcagc caggataagc ctgactggta actttcaagg cacagcaatt
     4921 caaaaaaatc tctatcatca tccaggggtt gaccccattc taaatcgtgg taatcacagt
     4981 aaagctggtt atccttagga acccatgagc aacgtttcat gtgaaacacc taccctttca
     5041 ttaacagttt caatgctgat ttgatgtatt gctctgccgt ctcagaggtc ccctcaaaga
     5101 aggcacgaat ttttttcagc tcttttgctt tataaccgag agcaaggagg gcttcaatcg
     5161 cttcgtccag ttgagtattg cctgctttat tgctgcgcgc tttggtatga ccagtctctt
     5221 gtggagcctc cacaaatttg ccagctaagt caagaaccat ttgctgcgct gtttttttac
     5281 caattttagg aaatttcatt aggtacttaa tgtcactgtt atcaatggca ttcacaagtc
     5341 cctcattatc atcaactgcc acaatagcaa gagctgttgt cggaccaata cctgatacag
     5401 aaattaattt cagaaaaaca tctttttcat cttccgtgtg aaacccaaac aacaggtgag
     5461 catcctcacg aatcacttga tgcagataaa tggttaccaa ttggttgaca ctatctgtaa
     5521 agctataagg attggcaaca ttaatcatgt agcccaatcc attagcttca acgacaatgt
     5581 attttgccgt aattttggtc aattgacctt taatataatc gtacatatta cacctttttc
     5641 atttttgcag aaacaaccgt aaaataaatc cctagcaaag caatgactac aaataagcat
     5701 tgcacgccta aaggacctgt aaaatggctg acagatacta ggagactagc caaaatagca
     5761 cctaggggtt gtgccattga cacaaaacct gtataagatc ctatttggga atgatccatc
     5821 atatcagcac gtaggacttg actagcagga acatttatca tttccccaaa agttaacaaa
     5881 acaactgata accagatagc atagaactgc gtaaaggtaa atgccaagag catccctaga
     5941 gtaaatagta aagaaccaac cacaagctga ggtaatagtt tccatttttc cgttaattta
     6001 tttactactg tcatgaaaag gacaatcagc aatgtattag ttaaaaccat taatgataac
     6061 attttagaac tagttacttg gaaacctaac acagccgttg gctgaaaata cagtttcaaa
     6121 tggactggca catagttatc catctgcatc cagatactac cagaaaagat ggcacctaag
     6181 gtaaacaaga caaaggcacg atcctgaaac acctgacggt agttctgaaa actcgccagt
     6241 aatcctttac catgatcaaa agcgtgtgtt tctggtctag tctcatcaaa atagtagtaa
     6301 gcaacgccaa aacaaagtac attaacgagt aataaggcta ctaacaaagc taaaaaatga
     6361 tggtcataaa aaagcccaga taaccctgca ccaaacatga cggcaatatt gataaaccaa
     6421 taattgatgg tgtaaacaaa tcgacgatta ctctcatcag tcaaatcaat taacatagct
     6481 tcataggcag gaccataaaa actagaagca atctctacca ataaaatacc cgcaaaggtt
     6541 aaccaaggaa tagctgcatt aggcaaatta gctaaaatag tcagaaacca gcctagtgtt
     6601 gttcctactg acccaatcat aatgactttt ttgcgaccaa gagcatctga cagatgccca
     6661 ccgtataaag ttccaacaaa tcccatcaaa ctggtaatca tcattaagag gcctgtccaa
     6721 aacgtaccaa agtaagtcgt ataatacatg gccataaagg gaaagatact actgcctaag
     6781 gtaatggtca caaagcgtac cagttgcctc agctgaatct gcttaggaag gtttaaaaac
     6841 tcttgcatct catcctctct aatattttcc tagctctcgc aggctagtgt gattttcttg
     6901 aattcgacgg aacatttttt ccatatctgc cttactaaaa ttaatcaata cgggtctacc
     6961 atgagggcag ttataaggat tttgacattg ggccaattgc agtagcagat ttcttgctga
     7021 ataatcatcc aagctatgat ttgccttgat agatcgttta caactcatca taatagctaa
     7081 ctctgctcgg taagttttga tagatacctc attggttaga agcaacatgt cacacatttc
     7141 atagacacca gatgcaatct cttcttcttt catccagata ggatgctccc tcaaaatgaa
     7201 agtattgtgc ccataaactt ctaagaagat accgacttca tttaagagtg ccattttctc
     7261 ctgtaagtta atgaaatcag aaccagaaaa ctcaaacaaa tagggcacca acaattgctg
     7321 taaactactg tcaacctcac ctatcttatc acgataatat tcgtatttaa cccgctcctg
     7381 ggcagcgtgt tggtcaatta taaataaacc atcttttccc tgagcaaaga gataagttcc
     7441 gtgcatctga ccaaaataat ccaattcggg aaacactgat tgcccttcat tttccaaacg
     7501 ggttagcatt tgagataact tttgtctgtt ttttaagtca agattaggat gatcagtctc
     7561 ggtaaaggta ttctggggac gacttgcgtg tttaaccgaa gaaggtccta taacttcttc
     7621 tgagtggggt aacagctcta ctttttcaac ctttacactg ttgtcaacag cgccggaata
     7681 aaaatcagtt tcaggtattt tttccgagac agccgactct ttgacaaaaa agtcattctt
     7741 ttgaggatca taataaagtc ccctagactg taaaggaagt tgtgtttgct ctggtttaga
     7801 gaaatgtcgt gtgcttgatt tggctaagtt ttccagagca tctggaatca aatcttgttc
     7861 cttaagactc tctgaaatag ccgtgctaat taaagccatt aattcacgct cttttgaaat
     7921 acgaacctct tgttttgtgg ggtgaacatt gacatcggcc aaatagggat caatctgaat
     7981 atcaatcaca acaattggga aacgtccaac catgagctta gaaccgtaac catcaagaat
     8041 tgctcgatta agcaagaaat tcttgatgta acgcccatta accaaaatcg tcatgtaatt
     8101 acggttggcg cgtgtcaact ctggcaagct aacatagccg gaaacttcaa aatccaaatc
     8161 agcattagag atagccagca tttttttggt agtattcaaa ccataaatcc ctgcaattgc
     8221 ttggcgtaaa tcgctagttc ctgatgtctg agttaattgg cgaccatcac taatcagtgt
     8281 gaaagcaact tctggatgtg ccaaactcaa ccggttgacc acatctacaa tgtgagctaa
     8341 ttctgcctgt aaacttttca tatatttgag acgagcaggc gtgttgtaaa aaaggttttc
     8401 aaccttaatt ttggttccag taggagttga gatcgcttca agtgtctcaa tttccccacc
     8461 agtagctata agaagagaac cgtgagtgac ttcttttgtt gctgttttta tcgtgatttt
     8521 actgatagag gcaacagacg gtaaagcctc tccacggaag ccaagtgttc taattctaaa
     8581 caaatcactc tgactcttaa ttttactagt agcgtggcga cgcagactta aaggtaaatc
     8641 ttcatgagac attccctcac cgttgtctgt aacctgtatc atcttaaggc cagactcttc
     8701 aatttcaacg gtaatctggc tacttttagc atcaatagca ttctcaacca attccttgac
     8761 aacactcgct ggcctttcta caacttcacc agctgcaatt tggttggcga gaacttccgg
     8821 taattcaata atgtttgtca taatttcctc gcattcttat ggactattat aacacactta
     8881 ataaatagcc gaaggcctcg gaccgttttt gacagaacaa aaagatgctt tctatattga
     8941 aggcatcttt ttcattttca gctgaaagac tataacaact ttttcaattc gtaaagggtt
     9001 gtcattgctt gaagcggggt catgttcatg acatcaatag cttccagtgc ttgcctaatc
     9061 tcatgagctt tctcttcatc accaaaaagg gataattgcc cctgtctaac agcgctgctt
     9121 gactcaactt gtgaagggac tgatattatc tcagcagatt gtgcttgcgc ttctaaacga
     9181 gtaagcacct catctgctct gcttagcagg gcctcaggaa gtcctgctat ttttgctaca
     9241 tgaataccgt aagatttatc cgccggtccc tcagcaatct tatgaaggaa ggtaacatcg
     9301 ccatcttttt caagcgttgc tacatgaaca ttgactaggc ttgtcaactt agttgacaag
     9361 tctgtcaatt catgataatg cgttgcaaat atggtcttag caccaactct atcatggata
     9421 tattcaataa ttgcctgggc taaagccata ccatcataag ttgccgtgcc tcgtcccagt
     9481 tcatcaaata gaataagaga gttgtcactt gcgcgtttga ttgcttggtt tgcttccatc
     9541 atctccacca taaaggttga ttgcccagaa atcaaatcat cagcagcccc aatacgcgta
     9601 aaaatcgcat caaataaagg taaatcaaca tgatcagcag ccacaaatga acccatctgg
     9661 gccatgataa ccgttaaggc cagctgtctc atataagtcg acttaccact catatttgga
     9721 cctgtaatca gctgaatact ggtctgttgg tcaaaagaga tactattggg aatgtattcc
     9781 tgcactccca taaccttttc aacaacggcg tgacgacctt cttgaattgt aatcacatga
     9841 ttatcattga actgcggccg aatataatga ttggtttcag caacgactgc taaactttgc
     9901 aaaacatcca ccgttgccaa agttttagcc agtttctgta aacgattaat ataggtttca
     9961 acttgagctc taatacacat aaaaatatcg tattctaaac tagatgactc ttccctagcc
    10021 tctaacatct gaccttcaat cttagccaat tctgctgttc cataacgttc agaatttttt
    10081 aaagttgcct ttctgaaaaa atgctcaggc actaagctaa gatttgaagt cgtaacgtgg
    10141 aaataatatc catctttttt attgtaatca atttttaggt tattaatgcc actttcttga
    10201 cgctctttgg cctcaatatc cgcaatccag cctgtcccct ctcgcattac tttacgataa
    10261 tggtccaagc gctcatcaaa accattgcgg ataatacttc cttcactaat agttgctggt
    10321 gcatctggat caatggctgt tctaatcaag tattccaact caggcaaact gtcaatatca
    10381 ttgacaagtt tgtcaataca aggactgtca aaagactcta agatagcttt gatataaggc
    10441 acttgggcta aggtatgccc caattgaagt aaatctttcg gatttgcctt gccaaaagac
    10501 acgcgactag ataagcgttc gatgtcgtaa acacctttta aactattgct taaatcggtt
    10561 cgctcaataa aagcattcag aaaaacttga ataatttctt gacgctctaa aatagcttct
    10621 ttagaaacca aaggtcgatc aatccatgag cgcaaaagcc tcatccccat agctgtcttg
    10681 gtttcatcta acagccaata gagacttcca tgttttttat tagtcctagc attttctacc
    10741 aaatctaaac ttgacttagt ggcatacgac atctgcaaat aatccttgat ctcatagtga
    10801 accaatgctt gcaagtggct gagttctcgc atttgtgttt tgtgaacgta ttgcaagagt
    10861 tttcctgccg ctgtcagttc taccgttgtc aattggccgt caattaaaga tttatcttca
    10921 tagaccgttt cttcataaga aagcagcaaa ttcatctgct tgaccaaaat cgtctgttct
    10981 tcttcagata aatcaaaacc tagtaagact tcttttgcct tgaggttttg gatttcgcta
    11041 cgaacactcg taaagtccgc caaatctgtc acgcaaaatt cacctgtgga tacatccata
    11101 taagccaatc cataacggca accatcaaag tcaacagcta ccaaaaagtt attggcgcta
    11161 tctggcttag ctgaatccac aaccgttcca ggagttatga cttgaacgac ctcacgcttc
    11221 accaccccaa cagcttgctt tgggtcttcc atttgttctg cgacagcaac cttgtaaccc
    11281 aactcaatta acacatcaat gtattgttgg gcagaatgat gtggcacgcc tgccatagga
    11341 attggatttt ccgcattctt gttgcgactg gtcaaaccaa tttctaagag ttgtgctgct
    11401 ttgacagcgt cctcgtaaaa taattcataa aagtcaccca tcctaaaaag caaaaaagca
    11461 tctggataat cttttttgat gtccagatac tgttgcattc caggagaaat gttagttttt
    11521 gccatttgtt aactccttgt ttaactgatc ttctatacgt ttggtgatat actgaatcac
    11581 atcacttgca tcttgcatct tttctcgata atcatcaatt aatggctgat gactgagcct
    11641 atcttctagt tcttttgccc tgcttgcaga tactgtcgca gccttttctt tatctatttt
    11701 ttttgaaaag cagggcatct tgttgatagc gtttcatctg ataagcttac tttttaaaat
    11761 catggagctt agacagctat tgcttcactt ctttataagc aacgatacta gaattcatgg
    11821 atagcaagaa tcaattgtgt caaagactct tgatagctag tcataggcct gcagccaaat
    11881 ctttttcaaa gcgtttagca atctcttcgt ctctgcaaat cacaagcaag gtatccgcac
    11941 cagcaatggt tcctagaata tcttcaatgg catcattgtc aatcaaatta gctagaacat
    12001 cagcctctcc taaattggta tggagaacca gcataaaacc tgctcgatca actttcaaaa
    12061 cataaaaacg cacctcagga ctaaatggtg ttgcaacggg ttcagacaaa ctataataga
    12121 gtttgccttg ttcatctctc aatttcaaaa ggccaatttc tcttaagtcc cgagatagag
    12181 ttgcttgggt gaccacaatg ccttcttttt gaaggtgatt tttaatatct tcttgtgtgc
    12241 caatatgctc tgcttgaatg attcgcttaa tctgctgttg acgctccatt ttattcatga
    12301 tatatcaccc caattgtata attatcctct caaattataa caaaaaaagt gtattatttc
    12361 atcttcgccc agctattttc agcaattttt tatcaaattc aactagaatt tcacttgaaa
    12421 actgttgaaa agaaacctca tttctctttt tataacattt tcagaaaaac aacaacctca
    12481 agcaattcat ttccttgaaa gtttcagtat aatttatcaa tcctctcctc gtttatttta
    12541 aagaaatatg ttagaatagg gtgacaaaca atttttagga gacgctgtat ggatactaaa
    12601 actctaatcg caagtgaaat agctaaagtt gttccagaat tagaacaaga tgctattttt
    12661 aacttgcttg aaaccccaaa aaattctgac atgggagacc ttgcctttcc agcctttagt
    12721 ttagcaaaag tccttcgcaa agcccctcaa atgattgcta gcgagttagc tgaacagatt
    12781 gatgagagcc aatttgaaaa agtcgtagct gtcggccctt acatcaactt tttccttgat
    12841 aaagctaaga tttcctcaca ggtccttgaa caagtgatta cagcaggttc agactatgct
    12901 cagcaagacg aaggtcaagg gcgaaatgtt gctattgaca tgtcaagccc aaacatcgct
    12961 aagccatttt caatcggtca cctccgctca acagttattg gtgatagtct cgctcacatt
    13021 tttgccaaaa tgggctataa acctgtaaaa atcaatcacc ttggtgactg gggtaaacaa
    13081 tttggtatgc tcattgttgc ctacaaaaaa tggggagatg aagctgctgt tcaggcacat
    13141 cctattgatg aactcttaaa actttacgtt cgtatcaatg ctgaagctga aactgatccc
    13201 accgttgatg aagaagctcg tgaatggttc cgtaaattgg aagatggcga caaagaagct
    13261 actgaattat ggcaatggtt ccgtgatgaa agtttacttg agttcaaccg actttatgtc
    13321 caattacatg tcaccttcga tagttacaac ggtgaggcct tctataatga caaaatggat
    13381 gaggttcttg acttattaga agctaaaaac cttcttgtag aatctaaagg tgcccaagtt
    13441 gttaaccttg aaaaatacgg cattgaacac cctgccttga tcaaaaaatc agatggcgct
    13501 actctttaca ttacccgtga cctagctgcc gcactttacc gtaagcgcac ttatgacttt
    13561 gctaaatccg tttatgttgt tggtaatgag caagctgctc acttcaaaca attaaaagca
    13621 gtgctaaaag aaatgggtta cgattggtca gatgacatga cacacgttgc ttttggactt
    13681 gtcacaaaag gcggtgctaa attatcaaca cgtaaaggta atgttattct tcttgaacct
    13741 acagttgctg aagccattaa ccgtgcagct tctcaaattg aagcgaaaaa tccaaatctt
    13801 tctgataaag aagctgttgc acacgctgtt ggtgtcggtg ccatcaagtt ttacgatctc
    13861 aaaacagatc gtatgaatgg gtatgacttt gaccttgaag ccatggtttc ctttgaaggt
    13921 gaaactggac cttatgttca atacgctcac gctcgtatcc aatctattct acgtaaagct
    13981 gactttacgc catctgcaac caccacttac agtttagctg atgctgaaag ctgggaaatt
    14041 atcaaactta tccaagattt cccacgcatt atcaagcgaa catcagataa ctttgagcct
    14101 tctattatgg ctaaatttgc tattaactta gcccaaagct ttaacaaata ttatgctcac
    14161 actcgcattc ttgacaacaa ctcagaacgt gataaccgct tagctctctg ctatgcaact
    14221 gctactgttc ttaaagaagc gcttcgctta ctcggtgttg acgcaccaaa tgaaatgtaa
    14281 gtaataacat tactaaggct gtcacattta tgacagtctt agttttttat attataataa
    14341 tagcacaata tgttacagga gaatgccatg ccatcagaaa aagaaatatt agatgctctt
    14401 agtaaagttt atagcgaaca agtaatacaa gcagatgact attttcgtca agcgatcttc
    14461 gagctcgcca gtcaacttga aaaagagggg atgagctcac tcctagctac aaaaattgac
    14521 tcacttatca atcaatacat cttaacacat caatttgatg ctcctaaaag catatttgat
    14581 cttagtcgct tggtaaaaac aaaagctagc cattacaagg gaactgctat atcagctatt
    14641 atgcttggta gtttcttaag tgggggacca aaataaaaat acaggctgtt tgacctgtat
    14701 ttttatttat cataatttct tcggcgaatt ttaaaatcag agctaataac ttcgtccaca
    14761 tcaataatgg agataaaggc atctggatcc aaaccagcta aaatacgttt gacttcccga
    14821 acttcagttg ggttcaaggt cacatacatg atattgtaat cattaccaga gtaagctcct
    14881 tgacctttca agtaagtcac tccacgattg atttcggtta ggatagcttg agcggcagcc
    14941 tctgaatgct tggtaataat aatcattcca cggatagtgt aaccaccatt ttgaaccaca
    15001 ctaatcattt ggctaaaaac aaagcttgcc accaaagtat aaagcatgtg ttttaaatcc
    15061 acataagtca acgaagctgt caatacaaga gcatccacca aaagcaaagt ttgacctagc
    15121 ttagctccaa atttttcttc tgcaatgcga ccaataatat ccgttccacc cgtggttgca
    15181 ccatagcgaa aaacaatgcc actcccaatc cctgaaaaga gacctgccac tacagccacc
    15241 aacatcatgt cattttccaa gccaagttcg ataggaactt tttgccacat ccagataaaa
    15301 gcggacatca gaactgttcc atagatggtt agagccaaag aacgcttccc aaaaatcttt
    15361 gcccctaaaa taaaaagtgg aagattaaat aagagagaag agtaagcagg attaatccca
    15421 aaatgcgcgt gtaaaatcaa ggttattcct gccacaccac cttcagccaa agcattagcc
    15481 atgttaaaat taacaaaacc gaaggtataa atggctacac ctagggcaat caaaaacagt
    15541 tttagtagtt tagtcaattt atcgtgatgt gccatccttt atcctcacta ttcttcgcag
    15601 gcaactctaa tgctcattta ttttctcttt ttatggtatt tgcttttaat agttaaaaat
    15661 tctgggctat tgacttcatt aacatcaaaa taagtgataa aggcattggg atcaacctga
    15721 gttaaaaaag cttttaattc cctaatatct cttcgcccca aagccacata aatcactttt
    15781 ttacggcgac cagagtaggc tccttcacct gttagataag taactcctct tcccaattcg
    15841 tccatgatga accgggcaat tttctccgct tgttctgaaa cgataaaaat gccacgggct
    15901 gaataaccac cttgttgtac caagcgaaca atttgaccat acataaagct agcaatcaga
    15961 gcatacatca tttgcggcag ggaaatataa gtcaaagaga caatcatgac aaaaagatca
    16021 atgcctaaaa gagcttgatt cagctgtagt cctaacttgt cttctaataa cttagcaata
    16081 atatctgctc caccaatggt tccaccattg cgaaacacaa gaccgccacc tagaccacca
    16141 ataattccag ccatcaagga aacgactaaa ttgtcatggt ccaagtcaac gaccaaagga
    16201 aagcgttgaa acagccacac aaaaaagtaa agtgataaaa tacccgttac tgtgtaaacc
    16261 atggctcttt tcccaaaaca tctagctccc agcaagacca aaggcaaatt aatcaaatag
    16321 cctatcaaag aaggatcaat ctcaaaaagt gccttaccaa ctagggtgag accagctaaa
    16381 ccattggctg caatgcgatt agccatgtaa aaataaacaa aagcaaaggc gtagatagca
    16441 gctcctaaaa taataaagca aaatttcttt aactgtaaac gttggtcaat tgacatgaca
    16501 cctcctgtgt ttttcttaat catcttccac aaaacgaccg ataattctaa cattctcagc
    16561 cactgaaaca aaggcttgtg ggtctgtttt taacatcagc catttgaatt ctgggtattc
    16621 ttcacatgtc aaaatggtaa tcaaaactgc tttttgttca tgcttgtaag tcccttctgc
    16681 gtcgttgata ctggtcaccc cgcgatgcag acgtttatgg atcatatgaa tcacacgctc
    16741 tggatggctg gtaacaatag ttgcctgcat tttcttttgc ttggtgaaaa tggcatccgt
    16801 aacacgactt gatacaaaga tagagaccat agaataaagg gcgtactgcc agccaaataa
    16861 aatccctgca aaggctaaaa ttgcaccatt aaccatcaat gagagactgc ccacatcctt
    16921 gcctgttcgt tttctaaggg tcaaactgac aatatcagtc cccccactag agatacgtga
    16981 tttgagacct gtaccaattc ccattcccat aaccaaacca ccaaaaatag cattgataag
    17041 aggatcagtc gtcagcgtca cttgaggtat gatttgaata aagaaagaac tcatgctgac
    17101 tgtgataaag gtaaaaatgg taaattgatg cccaatttta taccaagcta aaataagcaa
    17161 aggaatatta atcaaataaa acgcaaaggc gatgggaaaa tcatagccta aaagacgatg
    17221 actaagagct gaaaaaacct gtgctagacc agttgctcca cttgaataaa catgcccagg
    17281 ctggaagaaa aaattcacag caatactaga gagaatgcca taaagcagag aagccgaaat
    17341 cttctctgca tatttttctc ttgaaatact tcttagagcg tggagtaggc caactttctt
    17401 ggcccctcta ctaatgacat atttaacttt tttcttatag gttgttttct taatcatgac
    17461 tttctatttg aagactgagt tcttctaatt ggttttcaga aacaagacta ggtgcttgcg
    17521 tcattggatc agaggctttg ttatttttag ggaaggcaat aacttctcga atattgtctt
    17581 ttccagcaag caacatgaca aaacggtcaa gaccaattgc caagccacca tgaggaggaa
    17641 agccgtagtc catcgcttct agtaagaaac caaattgggc gttagcttca tcagcggtaa
    17701 agccaagagc cttgaacatg cgctcttgca tctctttttg attgatacgc aggctaccac
    17761 caccaagctc gtaaccattt aaaacgatgt cgtaagcaat agcccgtacc ttagccaaat
    17821 caccctctaa ctcatgagca gattctggcg ttggaagagt gaatgggtga tgagctgaca
    17881 tataacgtcc ttcttcttct gaccactcaa acattggcca atcaaccacc caaaggaaat
    17941 taaactgcga ctgatcaatc atgtcaaggt ccttggcaat acgattacga agggcaccta
    18001 atgtattatt agcaacctca agagtatccg ctacaaataa aactaaatca ttctcagcaa
    18061 gttttaattg gcttgacaac tctgtctcaa tagctgtcaa gaacttggct actgggcctg
    18121 ccaattgacc atctgtcacc ttaacccagg caaggccttt agcaccaaat tgtttggcaa
    18181 actcagttaa tttgtcaata tctttacgag agtatcgatc tgcatttccc ttcaccacaa
    18241 tcgccttgac agctggtgct tctgagaaaa ccttgaagtc aattcctttg acagttactg
    18301 tcaagtcttg taaaagcatt tcaaaacgtg tgtctggttt atcagagccg tagctattca
    18361 ttgctacatc gtagctcata cgtgggaatg gtagggttac gtctatttct tttgtctctt
    18421 tcatcacttt agcaatcatg ccttcaacga tgtcttgaat ttcttgttca gaaaggaatg
    18481 acgtttccaa gtcaacttgt gtaaactcag gttgacggtc accacgtaga tcttcatcgc
    18541 ggaaacattt cacaatttga taatagcgat caaagccagc attcatcaat aactgtttgg
    18601 ttatttgcgg actttgtggc aaagcataga aatgcccttg gctaacacga cttggtacca
    18661 gatagtcacg cgcaccttct ggagttgatt ttgtcaacat aggtgtctcg acatcgataa
    18721 actcaaggtc atcaaggtaa ttacggatag aatgagtcac ttttgcacgt agcttgaagt
    18781 tttctaacat ttctggacgt cgtaaatcta agtaacggta acgtaaacgc gtgtcatcac
    18841 ttacttcaac gtcatcttta atttcaaaag gcgtcgtttt agctgtgttt aaaatggtca
    18901 aagcagatac ttttaactca accattcctg tcgccaactt atcattggct tgttgtcgag
    18961 cttcaacaaa accttccact tcaatcacat attcactacg taagcgttca gcagttgcca
    19021 tcacatcact ggaaacttcc tcagggttaa tgactaattg catcacaccc tcacggtcac
    19081 gcaaatcaat aaagatgaga ccacccaagt cacgacgacg actgacccat cctttcaatg
    19141 tgattgtcgt tccaatgtgt tcttcgcgaa cacgccctgc atacatgcta cgtttcataa
    19201 ataaattccc taaaacaaac agaatcctgt gaaacctgct cgtttccaat tcctttcact
    19261 atataaatca attaattcat tccctactat tatagcaaaa agcccttaaa aaccccaccc
    19321 atttaaaaag agtttttcta aagataaagc aagaatactg agtcgtcagc attttgacat
    19381 atcaggtgtc ctcacagaat actaagaaag ctaatcaatc cccttacaga aaacaaacag
    19441 gtgcaactcc cttctaaaaa gagaagttac acctgtttta gtgattatct tctaaacaat
    19501 catatcaaca caagcttatg acgacaataa aatcattaag acaataattg ctctgagatg
    19561 ttttcaaagt tggtcatcat atcttcaaaa ctgacttcca attcttggcg gctacgatta
    19621 ttcttaatca cagctttgcc tgcttcaact tcactttctc ctaaagtcat taccaatttt
    19681 gctttaaagg tatcggctga tttaaactga gccttaatct tacggccaag gtaatctcgt
    19741 tcagctgtaa acccttgacg gcgaatggct tgaaccaatt ctaaagcttt gctattagca
    19801 ccatcaccta acacagcaag gtaaatatcc atctctgtct caattggcaa ggtaatgccc
    19861 tgcttttcaa taatcatcaa gagacgttct aaaccaagac caaagccaaa tcctggagtc
    19921 tcaggcccac caaaataacc taccaaacta tcataacgtc cacccgcaca gatagttaag
    19981 tcagaacctt ccacagacgt gatgaattca aaaatagtgt ggttatagta atcaaggcca
    20041 cgcaccatat tggtgtcaat cacataagga atgtcaagtg cttcaagcat atctttgacc
    20101 gcctcaaagt gcgcctgact ttcctcatct aagtaatcca aaatagaagg cgctttttca
    20161 acggctagtt tatcttcttt ttctttagaa tcaagcacgc gcagtgggtt ttcatccaag
    20221 cgacgttgac tatctttaga taattgatca cgcattggtg ttaagtagtc aatcaaggct
    20281 tgacgataag ctgcacgact ctcaggactc cccaaactat ttaaatgaag ggtgacatcc
    20341 ttaatgccca atttttcaaa gaggtgatag gccatcgcaa tggtttcaac atctgttgct
    20401 ggattagcag cgccgaaaca ttctacccca atctggtgaa attcacgtaa acgaccagct
    20461 tgaggacgtt cgtagcggaa catggaaccg atataataaa gtttgacggg tttttggacc
    20521 tctggcgcaa acaatttatt ttcaacataa gaacgcacca ctggagctgt accctctggt
    20581 cgcaaggtaa tgtgacgatc tcctttatca tagaaatcat acatttcctt cgtcacaata
    20641 tcagtcgtat cacctacaga ccgcgagatt acctcgtagt gttcaaacat aggagtacga
    20701 atttctccat aattatattg gctaaacgta tcacgcgcaa cagattccac atactgccat
    20761 ttcgcagcat ccccaggcag aatatcctgg gttccttttg gtttttgaag tttcataagt
    20821 ttacctcttt gattagtttt ctctttaaac attctaccat tttttcttat attttaaaca
    20881 agcatatatc atttgcttta gtttgatagt attttcacca tgaaattgta aaaataaaaa
    20941 gttttgtcaa gtaactttac tttacaaaaa agatatgtta tcatagtgaa gttgatgaaa
    21001 atcaaaactt acacatcgaa aggaagaaca agaaaatggc agtacctgca cgtcacacgt
    21061 caaaagcgaa gaaaaacaaa cgtcgtacac actacaaatt gacagctcca tctgtacaat
    21121 tcgacgaaac tactggagat tactcacgtt ctcaccgtgt atcccttaaa ggatactaca
    21181 aaggacgtaa aatcgctaaa gctaacgaag ctaaataata gaagggagat accatgcgcg
    21241 taaatattac acttgaacat aaagaatctg gtgaacgctt gtaccttact tcaaaaaaca
    21301 aacgtaacac tccagaccgt cttcaattga aaaaatactc accaaaatta cgtaaacacg
    21361 ttacatttac agaggttaag taaggggttc actacacgtc attagacgtc aaaacattga
    21421 ttttaagcga tttttgaatt tttgaagttc attacattgt aatataaatc aataagaact
    21481 ctacctttta ctctaccttt tttagatgaa tatatcattt ggatattgaa gttaggccct
    21541 gttatcattt cgatggcagg gctttttaat atttggtaca ctaactactt atctatattt
    21601 tggtggagct gaagttgaca tctataaagt ttaatgttaa tatacattca aaaatacatt
    21661 tgaatgaggt gttttatgat tcaaaatgtt gttacttcaa taatcctgta ttctgggaca
    21721 gccgtagact tacttattat cctaatgtta ttttttgcca aaagaaaaag cagaaaagac
    21781 atcattaaca tctatttagg acaatttcta ggctctgtta gtctaatatt gctaagtttg
    21841 ctttttgcat ttgtcttaga ttatattcct agtaaagaga ttttaggttt gctcggtttg
    21901 attccaattt tcctaggcct caaagttttg cttttaggag attctgatgg agaagctatt
    21961 gccaaagaag gtttgcgcaa agataataaa aacctgattt ttctagtcgc tatgattact
    22021 tttgcaagtt gtggtgctga caatattggt gtctttgtcc cgtattttac taccttaaat
    22081 ttagcgaatt tgatagtggc tttacttacc tttctagtca tgatttatct cttggttttt
    22141 tctgcccaaa aattggcaca agtcccttct gttggagaaa ttttggaaaa atatagcaga
    22201 tggtttattg ccgttgttta tttaggattg gggatatata tcctgattga aaacaacagt
    22261 tttgacatgc tatgggctgt gttaggctag gagaaaatat tatgaaaaaa gatagtatct
    22321 gccaagtgaa tgttataaat caacaaaatg ttacaaccgc aacgaactac cttgaaaagg
    22381 aaaaagtcca aaaatcactt cgtattttat caaagtttac cgataataaa cagataaata
    22441 tcatctttta tctc
//
LOCUS       DAUCWU010000018        22379 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUCWU010000018 DAUCWU010000000
VERSION     DAUCWU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22379
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.018"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            68..1300
                     /locus_tag="VGY24_000695"
     CDS             68..1300
                     /locus_tag="VGY24_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4283697.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFIGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1468..2649
                     /locus_tag="VGY24_000696"
     CDS             1468..2649
                     /locus_tag="VGY24_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4283698.1"
                     /translation="MTKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSSKQQHFKATKKQSYLERVEDFINHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2801..4219)
                     /gene="sufB"
                     /locus_tag="VGY24_000697"
     CDS             complement(2801..4219)
                     /gene="sufB"
                     /locus_tag="VGY24_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ4283699.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4212..4691)
                     /locus_tag="VGY24_000698"
     CDS             complement(4212..4691)
                     /locus_tag="VGY24_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ4283700.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4678..5904)
                     /locus_tag="VGY24_000699"
     CDS             complement(4678..5904)
                     /locus_tag="VGY24_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4283701.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(5935..7197)
                     /gene="sufD"
                     /locus_tag="VGY24_000700"
     CDS             complement(5935..7197)
                     /gene="sufD"
                     /locus_tag="VGY24_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ4283702.1"
                     /translation="MTKEKLVAFSQAHAEPSWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7292..8062)
                     /gene="sufC"
                     /locus_tag="VGY24_000701"
     CDS             complement(7292..8062)
                     /gene="sufC"
                     /locus_tag="VGY24_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ4283703.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     ILEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8184..9353)
                     /locus_tag="VGY24_000702"
     CDS             complement(8184..9353)
                     /locus_tag="VGY24_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ4283704.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9360..10121)
                     /gene="mecA"
                     /locus_tag="VGY24_000703"
     CDS             complement(9360..10121)
                     /gene="mecA"
                     /locus_tag="VGY24_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ4283705.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10267..11106)
                     /locus_tag="VGY24_000704"
     CDS             complement(10267..11106)
                     /locus_tag="VGY24_000704"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ4283706.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11172..13097)
                     /locus_tag="VGY24_000705"
     CDS             complement(11172..13097)
                     /locus_tag="VGY24_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ4283707.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLSQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVTSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13295..14863
                     /locus_tag="VGY24_000706"
     CDS             13295..14863
                     /locus_tag="VGY24_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ4283708.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEAKSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRYIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14856..15596
                     /locus_tag="VGY24_000707"
     CDS             14856..15596
                     /locus_tag="VGY24_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283709.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15738..15854
                     /locus_tag="VGY24_000708"
     CDS             15738..15854
                     /locus_tag="VGY24_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ4283710.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16080..17090)
                     /gene="gap"
                     /locus_tag="VGY24_000709"
     CDS             complement(16080..17090)
                     /gene="gap"
                     /locus_tag="VGY24_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ4283711.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17438..19516)
                     /gene="fusA"
                     /locus_tag="VGY24_000710"
     CDS             complement(17438..19516)
                     /gene="fusA"
                     /locus_tag="VGY24_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ4283712.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20000..20470)
                     /gene="rpsG"
                     /locus_tag="VGY24_000711"
     CDS             complement(20000..20470)
                     /gene="rpsG"
                     /locus_tag="VGY24_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ4283713.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20491..20904)
                     /gene="rpsL"
                     /locus_tag="VGY24_000712"
     CDS             complement(20491..20904)
                     /gene="rpsL"
                     /locus_tag="VGY24_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ4283714.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21177..22310)
                     /locus_tag="VGY24_000713"
     CDS             complement(21177..22310)
                     /locus_tag="VGY24_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEQ4283715.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLD
                     AVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
                     TIDIHKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEE
                     LQENAQYYQTVEKSRGQIEVREYWVSSDIKWLCQNHPKWHKLRGIGMTRNTIDKDGQL
                     SQENRYFIFSFKPDVLTFANCVRGHWQIESMHWLLDVVYHEDYHQTLDKRAAFNLNLI
                     RKMCLYFLKVMVFPKKDLSYRRKQRYISVHLEDYLVQLFGERG"
BASE COUNT         6779 a         4767 c         4063 g         6770 t
ORIGIN      
        1 agtcattacc ttagacaaaa atatggtata attaaaaaaa taatacttga gtaaggataa
       61 tctataaatg atcaaacgat taatttccct agtggtcatc gccttatttt ttgcagcaag
      121 cactgttagc ggtgaagagt attcggtaac tgctaagcat gcgattgccg ttgaccttga
      181 aagtggcaaa gttttatacg aaaaagatgc taaagaagtt gtcccagtcg cctcagtcag
      241 taagctcttg acaacctatc tggtttacaa agaagtttct aagggcaagc taaattggga
      301 tagtcctgta actatttcta actaccctta tgaactcact acaaactata ctattagtaa
      361 cgttcctctt gataagagaa aatataccgt taaagaactt ttaagtgcgt tagttgttaa
      421 taacgccaat agccccgcta ttgctttagc tgaaaaaata ggcggaaccg aacccaaatt
      481 tgttgacaaa atgaaaaaac aattaaggca atggggcatt tccgatgcaa aggtcgttaa
      541 ttcaactggc ttaactaacc attttttagg agctaatact tatcctaata cagaaccaga
      601 tgacgaaaat tgtttttgcg ccactgattt agctattatt gccaggcatc tcttattaga
      661 atttccagaa gtactgaaat tatctagcaa atcctccact atttttgatg gacaaaccat
      721 ttacagttat aattacatgc ttaaaggcat gccttgttat cgagaaggcg tggatggtct
      781 ttttattggt tattctaaaa aagccggtgc ttcttttgta gctactagtg tcgaaaatca
      841 aatgagggtt attacagtag ttttaaatgc tgatcaaagc cacgaggatg atttagctat
      901 atttaaaaca accaatcaat tgttgcagta ccttttaatt aattttcaaa aagtccagtt
      961 aattgaaaat aataaaccag taaaaacgtt atctgtctta gacagtcctg aaaaaactgt
     1021 caaacttgta gcccaaaata gtttattttt tatcaaacca atacatacaa agaccaaaaa
     1081 taccgtccat attactaaga aatcatccac aatgatcgca cctctatcaa agggacaagt
     1141 cttaggtaga gcaacccttc aagataaaca tcttattgga caaggttatc tggatactcc
     1201 tccttctatc aatcttatcc ttcaaaaaaa catttctaaa agtttctttt taaaggtctg
     1261 gtggaaccgt tttgtgaggt atgtcaatac ctctttatag aataacacaa tgctttttgt
     1321 atgacattga ctgacagagt acttctgcag tctttttcat ttttatttaa gcaaactagg
     1381 ccaatcctaa caatttttca aaaaaacttc acattgtatt agcaaagtga tatactgaaa
     1441 atgcattaca tttaaaggag tcggaaattg accaaaagaa ttatactaac tatttttacc
     1501 tttatttgct tttcggtgat gcctcttgtc cacgccgaag atgtcatgga tattacaagg
     1561 caagcaggct acacagttag tgaggttaat cgaccaaaat cttctattgt cgttgatgca
     1621 aatagttcag atattctttg gcaagataat attgatattc ctagggatcc tgctagtatg
     1681 tctaaaatgt ttacgcttta cattttattt gaagagctcg ctaagggaaa aattacgatg
     1741 gacactacta ttacggcaac tccaaccgat caagctatcg ctaacattta tgagattagc
     1801 aataataata tcgtcgctgg agtagcttat cctattcgtg atcttattac tatgacagct
     1861 gtgccttcat caaatgcagc aactgttatg attgccaatt atttatcaaa caatgatgct
     1921 tctgcgttta tcgatcgtat taatgccaca gccaaacaat taggcatgac taacactcat
     1981 ttttcaaacg ctagtggtgc agcagcacaa gcttttcaag gttattataa tcctactaaa
     2041 tatgatttat ctgcttcaaa tattacgacc gctcgagatt tgtccaagtt actctatgcc
     2101 ttcttaaaaa aataccctga aattatctcc tttacaaata aatctgttgt gcacactatg
     2161 gtaggaacgc catatgaaga agaatttcac acctataacc actctctccc agataatcaa
     2221 tttggtatga aaggagttga tggattaaaa acaggttcta gtcccagtgc tgcttttaac
     2281 gccatgataa ctgccaaaag aggcaaaact cgactcatta ctattgtcat gggggtcgga
     2341 gattggtcag atcaaaacgg ggagttttat agacatcctt tcgtcaatgc cttgactgag
     2401 aaagggttta aagatagtaa gaccttatcc aaaaaagcac gccaaaaact cgaaaaatta
     2461 gtgccacaaa ccaaaaaaga aacatcatct aaacagcagc acttcaaagc cactaaaaag
     2521 caatcgtact tggaaagagt cgaagatttt ataaatcaca accacacttt cttgcttatt
     2581 tgcttagcta tctttataat tactatcctt ttactaagcc tcgttgtctt tgcaatggga
     2641 cgccaataat gaattatcaa aaacctctgt caaagcacag aggtttttga tatgttatct
     2701 tacgtgttta aggcaactaa aaactaacca cttggggtta gtactacaac tcataaaatc
     2761 ctatctcaaa gatctttcag atggataaga gaaaatgcaa ttaaccgaca gaaccttcca
     2821 tctcatagga aatcaatcga ttgagttcga ccgcgtattc cattggcaat tctttggtaa
     2881 aaggctccac aaatcccatg acaatcatct ctgtggcttc gctttctgat aagccacggc
     2941 tcatgagata gtagagttgt tcttcagaaa tcttagacac tttggcttca tgttctaacg
     3001 ccacctgtga attatgaatt tcgttaaatg ggatcgtatc tgatttagaa atatcgtcca
     3061 tcaaaatagt atcacattca atatgggaaa cggatttttt agattgcttg ttaaaggtca
     3121 cttggccacg ataatctact ttaccaccag acttggcaat tgattttgac acaattgatg
     3181 atgatgtatg tggagcattg tgaatcattt tagcccctgt atcttggtgt tggcctgcgt
     3241 tagcaaaggc aatagacagc atggtgccac gcgcacctgg cccatcaaga taaacagaag
     3301 ggtacttcat ggtggtttta gctcctagat tgccatcaat ccattccact gttgcatccg
     3361 taagggcgcg tgcacgtttt gtcactagat tatacacatt gtctgaccag ttttgaatgg
     3421 tggtataacg catgtatgca ccgtcaagcg caaagatttc aacgatagca gcatgtaagc
     3481 tgttacttga ataagtggga gctgtacatc cttcaacata atgaacactt gctccttcat
     3541 caacaataat caatgtccgc tcaaattgac cagtattttc attgttaatg cggaaataag
     3601 tttgcaaagg gatatctacc ttgacccctt taggaacata aataaaggta ccaccagacc
     3661 aaactgctga attgagggct gctaatttgt tgtctgttgg cggaaccagt ttggcaaagt
     3721 attgtttgaa caaatcagga tattctttga gggcggaatc ggtatctgta aagataatcc
     3781 ctagcttttc aaattcaccc ttcatgttat ggtaaaccac ttctgactca tactgagctg
     3841 atgctccagc aagataagca cgttctgctt ctggaattcc aatacgatca aaggtttctt
     3901 ttattttttc tggaacatca tcccaagaac gagctggctt atcagatgct ttttgatagt
     3961 aaatgatatc atcaaagtta atatctgata agtctgctcc ccaggtttgc atcggcattt
     4021 tattaaaggt ttccaaggat tttaaacgaa actccaacat ccactcaggt tcatttttgg
     4081 cagctgatag ttcgcgaacc actgcctcac tcaatccttt tcctgtagaa taaatgggct
     4141 ctacatcatc gtgaaatcca aattggtagt cccctaagtc aattggcttt ggctctactt
     4201 tctcatttat atcagacatt tttcccttcc tttacatttt ggtccgtgag gtgctgagca
     4261 ttggcacttc gtttaatggc ttccttgaga gcattccaag ctagggtaga gcatttgata
     4321 cgctgtggaa attttgcaac tcctgccaac aattctgctt cacctagctc tttttgagca
     4381 ggattttcct gtccttgtac catctctgaa aaaatatcag ctaacgcgag tgcttcttct
     4441 ttacttttac caataacagc atctgtcatc atgcttgatg aagctgtgga aatggtacag
     4501 ccgttgcctg caaaagcaat atcttcaatt ttatcttcgt caaacttaac ggtcaaagaa
     4561 atcacatcac cacaagtcgg attattcagt tgaacagcct ctacgccatc tagttgccca
     4621 tgatgatgtg gacgtttcga atggtccgct accacagcca tgtatagatg gttcagttta
     4681 gagagtgcca ttgaaaaact cctttgcttt tagaattgct tcgactagac ggtcacaatc
     4741 tgccttggtg ttatagatat aaaaacttgc tctaacagtt gctggtacac ctaaataact
     4801 aagaagaggt tgggcgcagt ggtgccctgc tcttactgca acaccttcat agtccaaggc
     4861 tgttgccaag tcatgaggat gcaaatcatc cagattaaaa gaaatcagac cagatcttgc
     4921 actaggctgg cttggtccat atatggtaag cccctcaata gcttctaatt tcggcaagac
     4981 ataggctatt agttctgctt catgcgcatg tatatcagcc atgcctagtc tctgaaggta
     5041 agaaatggct gcgcttagcc caatagcacc agctatgtga ggtgttcctg cttcaaactt
     5101 ccagggcaat tctttccaag tggcctcttg ttcgtaaaca aaatcaatca tttctccgcc
     5161 aaattcaaga ggctccactt gattcaaaag ctcttctttg ccgtaaagaa cacccaaacc
     5221 tgttggcccc aacatcttat gagctgaaaa agcaaagaaa tcacaatcca agtcttgaac
     5281 atcaatagcc aaatgtggaa ccgactgggc accgtcaaca acaaggtagg ctcctttagc
     5341 atgtgccagc ttggcaattt ctttaatggg attgatgcaa ccaagaacat tggagacatg
     5401 tactaggcta acaaaacgtg tttttgtcgt caatttgttt gccaaatcgt ccatgtcaag
     5461 ttggccatct tttaaataaa catagactaa ccttgctcct gttttttgac aggcttgttg
     5521 ccaaggaatg atattggcat gatgctccat aatcgaaatc aacacctcat cttctggcgt
     5581 caagacctgc tctgcaaacc gagcaaccca attcaaactg gttgttgttc ctctggtaaa
     5641 gagcacttcc ttgcttgatt tagcatgaat aaagtcagca acctgctggc gactggcctc
     5701 atatttgtgc gttgcacgtt cagccaaggt atgaactcct cggtggacat tagcattatc
     5761 ttcttgataa taggattgca aagcctcaag aacaagcgcc ggtttttgtg tggtggcggc
     5821 attatccaaa taaacaaggg gttcatcatt gacttgttga tttaagattt gaaagtcttg
     5881 tttgatgtct tttgcgtcta gcaatactaa cctctttctt tttggcagta ggtcttaacg
     5941 attaagcaat ttctcatcta aaaccttaat aatctcttgg cggactgatg gaataggaat
     6001 ttcagcgata accgctccta ggaatcctct aataaccaat cgttctgctg tttcttgatc
     6061 cagtcctcga ctcatcaagt aatacatatc ttcagggtca acctgaccga tagaagctgc
     6121 atgacctgct gttacttcat tttcatcaat taagaggatt ggattggcat cggctcttgc
     6181 ttggtcagaa agcatcaaaa cacggctttc ttgttgagca tcagctccct tagcgccttt
     6241 tagaatatga ccaatcccgt taaacgttaa ggtgccacgt tccaaaatca caccatgctg
     6301 taaaatatga ccgaccgtac gttgaccata gttggtcacg cgcgtgtcaa taccttgtac
     6361 ctgacgacca cttgaggctg caacaacttt caaatcagct tgtgagccct gaccaatcaa
     6421 atcactgtca aaatcagcaa tgacattgcc ttcattcatc acagctaagg cccaatcaat
     6481 gttggcatcc ttctctaaac gtcctcgacg gctaatatag gttgtcactg aaggacctaa
     6541 gcggtcgata gccgagaatt taatctggct gcctgcttga gcaatcactt ctacactgat
     6601 attagcgctg atcttttgag tggcattgcc aatagattca aaacgctcta aataggtgaa
     6661 cttactttct tttcctgcaa tcactagaac atgcttgtta aaaggaacgt cactgtcact
     6721 atcttgtaag aaaatagctt caataggagt tgtgatttcc aagtgatcag gaacgtagag
     6781 cacggctgcg ctattaaaat aagcagtgtg gtaggcagct agtttgtctt catcaaaagc
     6841 taatgcctga ccaaaatgag cttcaattac ttctgggatt tcctcaagcg ccgtataaaa
     6901 atcactgaaa acaactccct tgtcaattaa cgccattggt aactgttcta agactgtttg
     6961 cgtgcctacc tgaacaagct ttgggttatc tccaatagct ataaaatctg gaacactagc
     7021 tagactttca ttttctgtta aggtaccatc tcctagattc caacggtgaa atttaaccct
     7081 ttcgatggtt ggtaactcta attctggtat ggcttctaat gccgctaaac gccgctcttg
     7141 cagccaagaa ggctcagcgt gggcttgcga aaaagccact agtttttctt ttgtcatctg
     7201 tcaatccttt gtctctggtt tttaaattgg ctgggtatat agaacatcac ctagcttttt
     7261 gtagaaagct aaagtaattg tctccaaaaa cttaactttc ttccttatac tcgattccca
     7321 gatcctgtgc aatacctgcg tagccttctt tttcaagacg agtagccaag gcagcatcac
     7381 cagataagac aatacgacca tccatcataa catgaactag gtctggggta atgtaattca
     7441 acaaacgttg atagtgggtg ataatcatag ctccaaagtc tttcccacgc atctcattaa
     7501 cccctttaga gactactttt agggcatcaa tatcaagacc agaatcaatt tcatctagca
     7561 aagcaaattt aggctctaat atgagcaatt gaagaatttc attgcgtttt ttctccccac
     7621 cagaaaaacc ttcattgagg taacgctctg ccatctcttc tttcatacct aacaaagcca
     7681 ttttctcatc aagtttggtg ataaaatctc gcacggaaat cttatcctca tctgccttac
     7741 cggcattcat tgctgcacgc ataaattcag cgttggtaat ccctggaatt tctgaagggt
     7801 attgcatggc gagaaaaagt cccagtctcg cacgctcatc aacttctaga tcgagaatat
     7861 tgaccccgtc aagcaaaatt tgcccttggg tcacttcgta gttaggattt cccataatag
     7921 ctgctgaaag ggtagacttt cctgtcccat ttggccccat aatggcagct acttccccag
     7981 ttttaagagt taggttaacc cctttcaaaa tctctttacc ttctattgaa acatgtaaat
     8041 tattaatctc aagaattgac atgatacacc ccttattttc tctgtcatct ataccatctc
     8101 aagaaaagac agtgtcattt gcactgcttt ttttataatg gtcactgcat ctattatagc
     8161 aaaaaaagcc ttactcggct tttttagtgt ttcaaatcct tcttttcttt ccatttcaga
     8221 agcattgctt gacgataatc actattacca ataaacttta acaagttaaa caaaggcgtt
     8281 cgcttttcac cccagatctc caatccttca ataaaaactt ctaaaccaaa taaaagtcca
     8341 agcatcaaga ggacgccacc aattcgacta gaaacattta aaagtaaaga aataagggaa
     8401 aaaagcatgg taataccata aactactaag acagctcctc gatgagtaaa gcccattgat
     8461 aagagtcgat gatgcaggtg catcttatcc ggctcgtaga atttttgacc tgataaacta
     8521 cgtcgaataa tagccacgat ggtatccata atgggtacac caagaataat aacaggtgtc
     8581 accacagcca cagccgttga atttttcaat ccttgaagag acaaaacccc tatcataaag
     8641 ccaataaaga gggctcctgt gtcacccaaa taaatcatag ctggatggta attataagga
     8701 aaaaatcctg caatagcaga aatcaagacc aaaatggtta acgtcaaaaa gaaatccttt
     8761 tgaggtaaaa agaaataaga tacaatagcc atggtcacta aactaataat agagacccca
     8821 ctaaccaaac catccaaacc gtcaatcaag ttaatagcat tagtgatgga aacaatccac
     8881 aggactgtta agaataaggt taagaaagga ccaaaaacta acaacggccc tccaaaagga
     8941 attttgaagc tatcaaattt aaagtcggta aaagcccaga caatcactgc accaattaga
     9001 ataccaaaca tctttaattt aggacttaac tcatacaaat catctaaaaa accagtaagg
     9061 gtaattactg tagcaccaat cacgacaggt aaaatataac tgaagtaggt ctgaccaaaa
     9121 aacatggcgc ctttagaagc aatcggaata agtcccaaac ttgctactaa aaaagacata
     9181 aaaatagcta atccaccact ggttggcatc ggaaccttat tgacccgtct ggcattggga
     9241 ttgtctactg cacccacacg aaaggctaaa aagcgaacaa gaggggttaa gaataaggac
     9301 attaacaaag cccctatcag aacaaggaca taatctattg tgaatgaaaa catactattt
     9361 tatcccaact caatcttttg taattgctca acagcaccat caagcatcaa ttgtaagcca
     9421 tgctcttgta aataagctct tgtttttgat ccagggtttg catgctcaat caatctcgca
     9481 tacatgacat tcgcaaaata agatggttgt tgctgaacat ctaataaaat agtcatatgg
     9541 tagcagttgc tccccttata taactctgaa gcttctatcg aaaaatcaat cgttttagca
     9601 aaagccactg cctctgtaat cgttgaaaaa tccaatacat aatggacata gtctaaaggt
     9661 tctgactcat gattagttga tggatcagcc gcttcagact gattagctaa agtggcttca
     9721 acaacatctt ccattatctc ttcaattttt tccaattttt catgagcttt gacatctccc
     9781 ttttcacgca tggattgctc caagctcttg aaaaaatctt ccggagtcat ttgggacata
     9841 tcaccaaact ctgctaaatc ttcaagatta atatctttat ttatctcaga tttggtaaca
     9901 aaaacatcta gacggtcctt gcgtggagtc actcgaaaac taagcatacc actatcttta
     9961 aaattatctg gaagatcgag ttcgtccatc acagaataga aaaactcttc tgttttttct
    10021 tgaggaatca agaaatcttt caactccatt cctctttctt ctaaatcgtc catactaatt
    10081 gttattttta gcgttgtctc gctaatttgt ttcatttcca ttctcaatac ctcgcacgcg
    10141 tagtcatttt attatactag aaatgctgta atttcgcaat ttatgactaa ctcctcttct
    10201 acaaaaggaa atttaaaaag cgacagtccc caaggtctgt cgcaacatcc taaacgatat
    10261 gtaagtttaa aaaacaaatt taaagaagct ataaataagt aaaaggcttc ctaacacaat
    10321 acggtatttc ccaaaaatgg tgaaatcgtg tttcttaaca taatctgtca agaacctaat
    10381 cgccaataaa ctaaccacaa aagcggtcaa gctagccact aaaagaataa ggacttgtgc
    10441 aaaatcaagg tgatggccat caaggaaaaa tttcacagcc tttaatccac tatatccaaa
    10501 catggtagga atggccaaaa agaaggtgaa atcagcggcc actgttcgac tagttcctaa
    10561 aataatagct cctaagatgg tagcccctga tcgactagta ccaggcacaa tacttaagac
    10621 ttgaaaacaa ccaatgaaga aagctgtttt gtaagacatt ctagctaatt cagtgacggc
    10681 aggttcttgt tgagcatttc gtttttcaat ccagataaag gcaatcccgt agacgattaa
    10741 agcgatggca atcggaacca taaaataaaa atgtgcttca aaccaattat caagaggaac
    10801 cgctattaaa atagaaggaa tacacgcaat caccactttt agccataatt gccaggtaag
    10861 ctgtacttct cttgctgttt tcccaggttg aaagggattg agacgttcaa aataaatcaa
    10921 cataacagcg ataatcgccc ctagttgaat aacgatatta aacatttcta taaaagcctt
    10981 atcctgattt agtcggataa attcctgcac taaaatgagg tgaccagtgc ttgaaactgg
    11041 taaccattct gtgattccct caatgatgcc aaagaaaatg gcttttagca attcgattat
    11101 taacatgttt tctccttatt aaccactctt attgtgattt tctaaactgt gattaatgcc
    11161 aagcagcttt tttaaaaggc accgccgccg cctcctccgc caccgccaga gaagccacca
    11221 ccaccactaa tcccgcctcc ggaagtgact gagaaatttg aggaagttgt agctgaagac
    11281 aagctagaca caaaggttgg tgtcgtagca tacattacca ttgataattc acctggtcga
    11341 acagcttggt aaacctcagg taaggcaata cgatggactt tcaaatagcg ttccactttc
    11401 ttagcgtaac caaataaggt ggcataaacc aaaacacggt tccaaacaac caaaccttca
    11461 atagcaacat cctcaaattt gtcaatgtct cgtatcatgt ttttaaagct ctgccattga
    11521 tgtaaccgaa cacccccctc aggtgtttca atacccaatt tcttgtattg atctaacttc
    11581 aggctaatgt aaaagccaag gcacatcgtt agcaccatta aagcaaggta aatgagggcc
    11641 aacccacttt ctttaatagc caagtaaatc aataaaccac tatttagaat aagcaacaaa
    11701 caacctaatc cctggacact attgcttttt cgtttctctg ccggggtcat ttgacgataa
    11761 gtatctggta agtttaactt tttgatagtt tctaggacat tgtttgtaat agctgccgac
    11821 gcttttttca tcgccttaat cacctgctca gacgattgac ggacttcttg ctcgagcttt
    11881 ttacctttgt acgttttctt taattgttta atggtatcag catcataatg gtattgacta
    11941 aacaattgat ccaccggcaa tgtcaccttg tttccaaaag ccatatctaa gaaggcagct
    12001 tcctcatctg tcacacgatc taactgactg atttcaagat aagctttccc ttcttctttc
    12061 gttaataaaa gcaccttttg gtcgatcaga tccaacaagg tagcttgaac aagggcctca
    12121 aaggtcactc ctttagggat caataatttc tggctttctg atgctgtagg ggataaatag
    12181 gcaaaactct gtccataaat cgcttgtgtc aacacaagag gcgaaagatc ttcagggact
    12241 tcgtaactgt gatctgtatg gttagccaaa tgataacgat tgaattgttt ctttaatctt
    12301 gtaaactgta ttaactgcga aagaagaaaa cctacctcta ccagaggaat gactttgcca
    12361 aatagcagct gtaacaaggt ccttcgtctg gaaatcttag tttctaacgc tacaatttta
    12421 ttctttttac tcgttgaaac atcaactggc tcttttccca ataatgcttt tttatcccaa
    12481 taagcatgca attccaattg ccctgagaca ttcttggcat taatctgata gcgactgtta
    12541 ccttctttta acacttgagg tttcttttgg taatatcctc tgtgagccca tagattagaa
    12601 tcctgaatat cagttggggt agtgacggtc aaactcacct tttctaatgt tttgtcccaa
    12661 tcactaatcg gtgtccaatt cagctctgcc acgtcttgat aagctgttaa aaggtggtgc
    12721 agttgccaga taaccttgac gtcaacctta tcaccagctt ggccagcatt ataaagtttt
    12781 aaacgatagc catctccaag gtcactaaac tcttgactaa caggtacctg ttgaccattc
    12841 tgatatacct caactttagg cttttggtca atagcaaagc ccgctggcaa atgacctgtc
    12901 cggcccagcg aaatatactg gccgttataa gaagtgtcaa attgataggt tactttttgc
    12961 tcaaaccgag cagtattttc ttttgataac agtaactgac cttcatagtt ggtgatgcta
    13021 tagtctacat cagctgcttt tattcgtatc cctaaaagac tgaagcagag cacaagcgtc
    13081 atcaaaattt ttttcatggc aacatcctct ttctgccctc attttaccat attttaagga
    13141 gatgacaaaa cctaaaccaa gtccttcatc ttatttttac tcattatttt gaatattttt
    13201 ccatatttca acctattcac tgataatata ttgaaatagg atgcaaattg ctgtaaaata
    13261 ggtctgaata ctatttttga aacaggagac ctatatgaaa aagctaatcc ttagctgttt
    13321 ggtcgccttg gcccttctgt ttggaggaat gagccgggct caagcaaacc aatatttaag
    13381 agtcggaatg gaagcagcct atgctccttt taactggact caagatgacg cttcaaacgg
    13441 ggctgtccct attgagggaa ctagccaata cgccaacggt tacgacgtcc aagtcgctaa
    13501 aaaagtcgct aaagctatga acaaagaact gttagtcgtt aagacctctt ggaccggttt
    13561 aattccagca ttaacttctg gaaaaatcga tatgatcgct gctggtatga gtcctaccaa
    13621 agagcgtaga aacgaaatta gcttctcaaa cagctactac actagccaac ctgttctagt
    13681 cgtaactgct aatggcaaat atgctgaggc aaaaagcctc aaggattttt ctggagctaa
    13741 agtaactgcc cagcaaggcg tttggcatgt caatctctta actcaactaa aaggtgctaa
    13801 gttacaaaca ccaatggggg atttctctca aatgcgacaa gcccttactt cgggtgttat
    13861 cgatgcttat atttctgaac ggcctgaagc catgactgct gaagctgctg atagccgttt
    13921 gaaaatgatc actcttaaaa aagggtttgc tgttgctgaa tcagatgctg ctatcgctgt
    13981 cggaatgaaa aaaaatgacg atcgtatggc aactgtcaac caagtgcttg aaggattttc
    14041 tcaaacggat cgtatggccc tgatggatga tatggttacc aaacaacccg tggaaaagaa
    14101 agccgaagat gctaaagcat catttctagg ccaaatgtgg gctattttta aaggtaactg
    14161 gaagcaattc ttacgtggaa ctggaatgac ccttctgatt tccatggtcg gaaccattac
    14221 aggtctcttt attggattat taatcggtat tttccgtaca gctcctaaag ctaagcataa
    14281 agtagctgcc ttgggacaaa aactctttgg ttggttactc actatttata tcgaaatctt
    14341 ccgtgggaca cctatgattg ttcaatctat ggttatctac tacggaacag cgcaagcctt
    14401 tggtatttcg atcgaccgta ccctagcggc tatttttatc gtatctatca atacgggtgc
    14461 ctatatgagt gaaattgttc gcggtggtat tttcgctgtc gacaaaggtc aattcgaagc
    14521 agcaactgct cttggcttta ctcacggaca aaccatgcgt aaaatcgtgc taccacaagt
    14581 tgttcgcaac attttaccag caacaggtaa tgagtttgtc atcaatatca aagatacttc
    14641 tgtcttgaat gttatctctg ttgtggaact ttacttctca ggtaataccg tagccacaca
    14701 aacctaccaa tatttccaaa cctttacgat tatcgccatt atctactttg ttcttacctt
    14761 cacggtgaca cgtatccttc gttatattga acgccgtttc gatgccgata cttacaccac
    14821 tggagcaaac caaatgcaga ttgcggaggt ctcaaatgtc taactcaatt attgaaatca
    14881 aaaatctcaa aaaatcttac ggcagcaatg aagtactcaa agatatctcc ctttctgtta
    14941 acaagggaga agtgatttcc attatcggtt cttctggttc tggaaaatca accctcctgc
    15001 gctcgatcaa tcttttggaa gagccaagtg ccggtcaaat cctctttcat ggtgaagatg
    15061 tccttgctga acactacaac ttgactcact accgtgaaaa actaggcatg gttttccaat
    15121 cctttaactt gtttgaaaac ctaaatgtat tggaaaatgc tatcgtcgct caaaccaccg
    15181 tcctcaaaag agaccgtgca caagccgaac aaatcgctaa agaaaacctc aatgcggttg
    15241 gtatgacaga acaatactgg caagccaaac caaaacaatt atcagggggg caaaaacaac
    15301 gggttgccat tgccagagcc ttatctgtca atccagaagc catgctcttt gatgaaccaa
    15361 catcagctct tgatcctgaa atggttggtg aggtccttaa aaccatgcaa gacttggcaa
    15421 aatcagggtt gaccatgatt attgttaccc atgaaatgga atttgctcgt gatgtttctg
    15481 accgtatcat cttcatggat aagggattga ttactgaaga aggaagtcct caacaaattt
    15541 tcgagaatcc tacacaagat cgtaccaaag aatttttaca acgcttctta aaataagata
    15601 aaggatccaa ctatttctat gccagttggg tcttttttca attcaaaaag aatttgtcat
    15661 aaaatacttt accagttaac tattttatgc tatacttatt tttgttaacg ttaataattt
    15721 atatttagga ggataatatg gcagaaaaaa ctgacttatc gtcagcttac agaagactga
    15781 aaagtcccaa tattaaaacc agaaaacgtg ctctgaagat tattcacgag tacaaacgtt
    15841 acggtaaaaa ataagatttt actttttatc tctccaaatt taagtcagac aaaaaaaggc
    15901 taagacaccc cttccatctt agcctttttg tatacagttt attaagggaa ctattttaaa
    15961 atatctttta tgctagagtc tagaatagtt caaaacgtta aaatcataca tttaaggtag
    16021 tgtttaaagt atacgaaaaa gaactcagca taaaccaagc tctctttcgt tataactaat
    16081 tatttagcaa tttttgcgaa gtactcaaga gtacgtacaa gttgagcagt gtaagacatt
    16141 tcgttgtcat accatgatac aactttaacc aattgtgatc cgtcaacttc cattacttta
    16201 gtttgagttg cgtcaaacaa tgaaccgtat gatacgccta cgatatctga agaaacgatt
    16261 ggatcttcag tgtaaccgaa gctatcgttt gaagcagctt tcatagcagc gttgatttcg
    16321 tcaacagaaa cgtttttgtc aagagttaca accaactcag ttactgatcc agttggaaca
    16381 ggaacacgtt gtgcagcacc gtcaagttta ccgttaagtt ctgggataac aagaccgata
    16441 gctttagcag caccagttga gttaggaacg atatttgcag caccagcgcg tgcacgacga
    16501 aggtcaccac cacggtgtgg tccgtcaagg atcatttggt caccagtgta agcgtggatt
    16561 gtagtcataa gacctttttg aataccgaat gcatcgtgaa gagctttagc cataggagct
    16621 aaacagtttg tagtacatga agcacctgag ataactgttt cagtaccgtc aagaatgtcg
    16681 tggttagtgt tgaaaacaac tgttttaaca tcgtttccac caggagctgt gataacaact
    16741 tttttagcac cgttagcatg taagtgtttt tcagctgctt cttttttagc aaagaaacca
    16801 gttgcttcaa gaacgatttc aaccccatca gttgcccagt cgatgttttc tggatcacgt
    16861 tcagcagaaa ctttgatgaa gtttccgttt acttcaaatc caccttcttt aacttcaact
    16921 gttccatcaa aacgaccttg agttgtatcg tatttcaaca agtgtgcaag catatttgga
    16981 tctgtaaggt cattgatacg agttacttca acaccttcga tgttttgaat acggcggaat
    17041 gcaagacgtc cgatacgacc gaaaccgtta ataccaactt taactaccat tagtgatttc
    17101 ctccttatga aaatcaaaag aatattattt agagggaatg ctccctacct attgtgaaaa
    17161 gattaactta cagtcatctg taacaacctt tcaacagaac tattatataa ctatttcaga
    17221 aaaaatgcaa ataaattagc cattttcata aagggcagct tctttcttct agccttcaaa
    17281 acccaccaaa tataggaaag ggctttcttt tttctatgtt ctattctgta atttatcttt
    17341 ttattgggaa agatttccaa aacttcaaac tgtactataa cgttctgtta cactaaaaaa
    17401 gaggttagga tagttatcct caacctctta gtggtggtta ttcacctttg tttttcttaa
    17461 tgatttcttc ttgaactgac ttaggaacat cttcatagtg gtcaaatacc atcatgaacg
    17521 taccacgtcc ttgagttgca gaacgaagaa ctgttgcata accgaacatt tcagcaagtg
    17581 gtacataagc acgaacgatt tggctgttac catgtgcttc cataccatca acgcgtccac
    17641 gacgagctgt cacgtgtccc ataacgtctc caaggttatc ttctggagct gtaatcgtta
    17701 caagcatcat cggctcaagg atagctggtt gtgcagattt agctgcttct ttaagtgcaa
    17761 gtgatgcagc gatcttgaag gcagtttcag atgagtcgac atcgtggtat gaaccatcgt
    17821 aaagcttagc tttaacatca accattgggt aacctgcaag aacaccgttt gccatagatt
    17881 cgatcaaccc tttttcaaca gctgggataa attcacgagg aactacacca ccaacgatag
    17941 cattttcgaa ttcgaaacct ttaccttctt catttggtgt aaattcaatc caaacatcac
    18001 cgaattgacc tttaccacca gattggcgtt tgaagaatcc acgagcttgt gtagaagcac
    18061 ggaatgtttc acggtaagat acttgaggag caccaacgtt agcttcaact ttgaactcac
    18121 gtttcatacg gtcaacaagg acatcaaggt gaagttcacc cataccagcg ataactgttt
    18181 caccagtttc aacgtttgtt tcaacgcgga atgttggatc ttcttcagca agtttttgaa
    18241 gggcaacacc catcttgtct tggtctgctt tagattttgg ctcaaccatc aactggataa
    18301 ctggttctgg aacttcgatt gactcaagaa taacttttgc tttttcatca gtcaatgagt
    18361 caccagttgt tgtgtctttc aaaccaacag cggcagcgat gtcaccagcg taaactgttt
    18421 caatttcttg acggctgttt gcgtgcattt gaaggatacg tccgatacgc tcacgtttac
    18481 cttttgatgt gttcataacg taagaaccgc tattaagaac acctgagtaa acacggaaga
    18541 atgtcaaacg acctacgaat gggtcagtca tgatcttgaa ggcaagagct gcaaatggct
    18601 cttcatcaga tgctggacgt tcttcttcag catctgtatc cgggttaaca cctttgattg
    18661 cagggatgtc aagtgggctt ggaaggtatg cgataaccgc atcaagcatc aattgaacac
    18721 ctttgttttt gaaggcagaa ccacaaagta ctgggaagaa ttcaacgttg attgttgctt
    18781 tacggatacc agcaatcaat tcgtcgttag tgatttcttc accttcaagg tatttcatca
    18841 tcaagtcttc atcagtttca gcaactgctt cgattaattt ttcacggtat tcttgagctt
    18901 gttctaagta ttcttctgga atatcttctt caagaatatc tgtaccaagg tcgttggtat
    18961 agatttctgc tttcatcttg atcaagtcaa tgataccacg gaaatcatct tctgcaccga
    19021 ttggcaattg gattgggtgt gcatttgctt gaagacggtc atgaagggtt tgtactgagt
    19081 aaaggaagtc agcaccgatt ttatccattt tgttggcgaa aacgatacgt ggaacaccgt
    19141 attcagttgc ttgacgccaa actgtttcag tttgaggctc aacacctgat tgtgagtcaa
    19201 gaacggttac agcaccatca agaacacgga gagaacgttg tacttcgatg gtgaagtcca
    19261 cgtgtcctgg tgtgtcgatg atgttaacgc ggtgaccatc ccattgagct gttgtagcgg
    19321 cagaagtaat agtgatacca cgttcttgct cttgctccat ccagtccatt tgtgatgcgc
    19381 cttcgtgtgt ttcaccgatt ttatgaattt taccagtgta gtaaaggata cgctctgttg
    19441 tagttgtttt accagcatcg acgtgagcca tgataccgat attacgagtt tttgcaagtg
    19501 aaaattcgcg agccatgtgt ttctcctatt tttttagttt actctttcat tatagcacta
    19561 tttagcaaaa acggataggc aggacctacc cgttatctaa atatttgttg gtaaagataa
    19621 tacctattgc ctttataagg cctggtatct tattcattga actcaggcta aacgctgtgt
    19681 gaaaaagata aactttccta gagacaaagg tttcttcatc aaatttccta ttttcacctt
    19741 gcgttctata cgccttcgta tcttattgat tgagttcgag ctacaaacca ctgacaaaaa
    19801 gataatagtt cctagaaact gaagtttctg cgtcactatt cctattttgt cttggacttg
    19861 attaacactc tcacatctta tattagttga attttagcta caaatgtctg ccaaaaagat
    19921 aaaattacct agaaactgaa gtttctgcgt caatttccct attttggcta gcatttgttt
    19981 gacgctaaaa tatcttatat taccaacgga agtgtgcgaa tgcacggtta gcttcagcca
    20041 tcttgtgagt gtcttcacgt ttcttaactg atgcacctgt gttgtttgca gcatccatga
    20101 tttctttagc aagacgatct ttcatagtgt gttcaccacg agcacgtgat gcgtttacca
    20161 accaacgaag tccaagtgtt gtacgacgtt ctggacgaac ttcaactggg acttggtagt
    20221 tagaaccacc gacacggcgt gcgcgtactt caagtacagg catgatgttg tccatagctg
    20281 tttcaaatac ttcaagagcg tcatttcctg ttgcttcttt gatagcgctg aaagcatcgt
    20341 aaacgatagt agcagctgta ccacgtttac cgtcaagcat aacacggttg ataagacgtg
    20401 ttacgatttt tgagttatat aatggatctg gtaatacttc gcgtttaggc gcttgatttt
    20461 tacgactcat tttctcttat cccccttcta ttatcctttt ggacgtttcg caccgtattt
    20521 agaacggcct tgtttacggt cagcaacacc tgcagtatca agtgcgccac gaacgatatg
    20581 gtaacgtacc cctggaaggt cttttacacg tccgccacgg ataagaacaa cgctgtgctc
    20641 ttgcaagttg tgtccgatac ctgggatgta ggcagttact tcgataaggt tgctcaaacg
    20701 tacacgagcg aatttacgaa gggctgagtt aggtttttta ggtgtcattg ttccaacacg
    20761 agtagcaacg ccacgttttt gtggggcagc cattttagtt tgaacttttt tgtgactgtt
    20821 gtaaccaatg ttcaaagctg gtgaatctga tttttcaatt ttagatttac gtggtttacg
    20881 taccaactgg ttaattgtag gcatctacat tctcctgtgt ttttttattt ttggttgata
    20941 tagcacttgg tgacagccca tatccggtgc actttttgca acaattgtca gcacgtctct
    21001 gtacactttt gagagaccaa aagtaaaaag taccgtctaa tataataaca cagacggtac
    21061 ttcatgtcaa atagaatttt ttctttgatc acgccgtacg catgaaaact ttctcttcat
    21121 ccttttgtgc ccggacatca attttgaaaa gattcctttt taaatcaatc atctgcttag
    21181 cctctttctc caaataattg gactaaataa tcttccaaat ggacagaaat ataccgttgt
    21241 ttgcgacgat aactgaggtc ttttttagga aataccatca ctttgagaaa atataagcac
    21301 atttttcgga taagatttag gttaaatgcg gctcttttat ccaatgtctg atgataatct
    21361 tcatgataaa caacgtccaa taaccagtgc atactctcta tctgccaatg acctcgtaca
    21421 caattggcaa atgtgaggac atccggctta aagctaaaga taaaataacg attctcttga
    21481 ctcagctgac catccttatc aatcgtgtta cgagtcatcc caataccacg taacttatgc
    21541 catttgggat ggttttgaca caaccatttg atatcggaag acacccagta ttctctaact
    21601 tcaatctgtc ccctagattt ttcaacagtc tgataatact gcgcattttc ttggagttct
    21661 tccaataagt tgacatcact aaaataaaga gcaatatcat cataaagtgt ttcttgattt
    21721 cctttgacgg ctaagcaata gtctgcttta cctttaataa tcgtatcaac gatagccgtc
    21781 tgcgtgccca ttgcgtctat cgttacaatg cttttatgga tatcaattgt ccgcaataac
    21841 tgaggaatgg caacaatttc attacttttc tcctcaaccg ctacctgtcc caaactaaga
    21901 tgatgacccc catcataagc cgttacaata tgaacaggct tctgattttt acctcgattg
    21961 cctcgaatcg ttttaccgtc cactgaaatc agttgatgaa cggcatctaa gcttgtcaat
    22021 gattgctcaa attgaacttt aagctctttt aaacggtctg aattaacaag actaatcaca
    22081 cgctctaagg tatcatgaga cggacaacct tcactcaaat caacgtaggt cgcaaacaat
    22141 ggttcattca tttcaataaa atcttccatc tctttccagg tttcaatgcc agctaactga
    22201 caaacgaaga caagaaatag aatggttgat aaggggtagc gaattttcca agattgacga
    22261 ctatccaatt caactgcgca atcatcaata gaaataataa aatcaatcat cgtagtcacc
    22321 tcatctatag tgtactactt tgattgattt tgtcattatg aatctagttt tctgaaaac
//
LOCUS       DAUCWU010000019        21724 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUCWU010000019 DAUCWU010000000
VERSION     DAUCWU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21724
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.019"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1221
                     /locus_tag="VGY24_000714"
     CDS             <1..1221
                     /locus_tag="VGY24_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283716.1"
                     /translation="LALFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAP
                     SVLVDLLSRFMGTIVIPYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKMI
                     CQSGYSKRSHNFYQTLLIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFI
                     LLLVYLLSYFNHSSKEYLKNELRREQQAYMTNLEIYGKHLEKLYRDVRAFQSDYLSRI
                     ERLGQAIKSESITQIQDIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIIS
                     AEKSGIDLNVEVPDNIKETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLGD
                     YQMFVVTNTTKKKVDINKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKE
                     FSQTLTMPKEEVDR"
     gene            1218..2501
                     /locus_tag="VGY24_000715"
     CDS             1218..2501
                     /locus_tag="VGY24_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283717.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSIIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            2505..3245
                     /locus_tag="VGY24_000716"
     CDS             2505..3245
                     /locus_tag="VGY24_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4283718.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            3785..4144
                     /gene="rnpA"
                     /locus_tag="VGY24_000717"
     CDS             3785..4144
                     /gene="rnpA"
                     /locus_tag="VGY24_000717"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ4283719.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            4110..4937
                     /locus_tag="VGY24_000718"
     CDS             4110..4937
                     /locus_tag="VGY24_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ4283720.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            4949..5863
                     /locus_tag="VGY24_000719"
     CDS             4949..5863
                     /locus_tag="VGY24_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ4283721.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTPANLAETVTERKAQQPSIPVEESEVPQDAGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKTVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            6177..6311
                     /gene="rpmH"
                     /locus_tag="VGY24_000720"
     CDS             6177..6311
                     /gene="rpmH"
                     /locus_tag="VGY24_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ4283722.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            6585..7289
                     /locus_tag="VGY24_000721"
     CDS             6585..7289
                     /locus_tag="VGY24_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ4283723.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMTEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALFKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            7338..8657
                     /locus_tag="VGY24_000722"
     CDS             7338..8657
                     /locus_tag="VGY24_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4283724.1"
                     /translation="MNMKKLASLAMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            8762..9649
                     /locus_tag="VGY24_000723"
     CDS             8762..9649
                     /locus_tag="VGY24_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4283725.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTEFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            9662..10492
                     /locus_tag="VGY24_000724"
     CDS             9662..10492
                     /locus_tag="VGY24_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ4283726.1"
                     /translation="MTKKKLTASDILTTVMLCVLTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            10649..11311
                     /locus_tag="VGY24_000725"
     CDS             10649..11311
                     /locus_tag="VGY24_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ4283727.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVKRDLSLSTLFKRSFLLAGLFFP
                     WSFAFLAFICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            11323..12237
                     /locus_tag="VGY24_000726"
     CDS             11323..12237
                     /locus_tag="VGY24_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ4283728.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIADKDLEKAKALQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            12259..13197
                     /locus_tag="VGY24_000727"
     CDS             12259..13197
                     /locus_tag="VGY24_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4283729.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDEGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(13308..14138)
                     /locus_tag="VGY24_000728"
     CDS             complement(13308..14138)
                     /locus_tag="VGY24_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ4283730.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNDNCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            14423..15214
                     /locus_tag="VGY24_000729"
     CDS             14423..15214
                     /locus_tag="VGY24_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ4283731.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALTLANKYANIYATIGWHPTEAGSYSEAVEEAIVSQLSHSKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKATTANAKRVFKLD"
     gene            15186..15776
                     /gene="rnmV"
                     /locus_tag="VGY24_000730"
     CDS             15186..15776
                     /gene="rnmV"
                     /locus_tag="VGY24_000730"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ4283732.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHARFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            15890..16762
                     /gene="rsmA"
                     /locus_tag="VGY24_000731"
     CDS             15890..16762
                     /gene="rsmA"
                     /locus_tag="VGY24_000731"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ4283733.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            17188..18060
                     /gene="rsgA"
                     /locus_tag="VGY24_000732"
     CDS             17188..18060
                     /gene="rsgA"
                     /locus_tag="VGY24_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ4283734.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRDTYKKVIKRK"
     gene            18070..18732
                     /locus_tag="VGY24_000733"
     CDS             18070..18732
                     /locus_tag="VGY24_000733"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ4283735.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHRAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKELSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            18725..19357
                     /locus_tag="VGY24_000734"
     CDS             18725..19357
                     /locus_tag="VGY24_000734"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ4283736.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFIAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            19359..20630
                     /locus_tag="VGY24_000735"
     CDS             19359..20630
                     /locus_tag="VGY24_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ4283737.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEKMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            20620..21558
                     /locus_tag="VGY24_000736"
     CDS             20620..21558
                     /locus_tag="VGY24_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ4283738.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPVNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
BASE COUNT         6463 a         3883 c         4676 g         6702 t
ORIGIN      
        1 ttagccctat ttttcaatca gttcccgtat ttgtcgccat tgctaattga tcctctatta
       61 tttttagttg ttttaaggca agaaaccaag caattatttt ctttgaaagc tctttttttg
      121 gctgttgcac ctagtgtttt ggtggatttg ctatctcgat ttatgggtac aatcgttatc
      181 ccctatcttt ttttgtcgag tggtatttat ctgggtcata ttatctttga cttgcttgct
      241 tatctgctga tttttccaag ttttgctatt attaattata tgattggaaa agattacaag
      301 atgatatgcc aatccggata ttcgaaacga tcgcataatt tctatcaaac attgctgatt
      361 tttgttttgg tttattatgt ggacattttt gtcattttag gatttacgga ccccttttta
      421 cattttcatc attctctttt tgttcccaca ccttataagt tattgttttt gatgtttata
      481 cttcttttag tttatcttct atcttatttt aatcatagtt ctaaagaata ccttaaaaat
      541 gagttgagaa gagaacaaca agcttatatg actaacttag agatttatgg gaaacatctt
      601 gagaaactct atcgagatgt aagagctttt caaagtgatt acctgagccg tatagagcgt
      661 ttaggtcaag ccattaagag tgaatccatt actcagattc aagatattta tgcccaaacg
      721 gttcatgaag ctaacgacta ctgggatgat aaacactata atatctcgaa gcttaggaaa
      781 attaatatct catcgattaa gagcttattg tcagctaaaa ttatcagtgc ggaaaaatca
      841 ggtattgatt tgaatgtgga agtgccagat aatataaaag agacttacat tcctgagttg
      901 gacttactat tgttgatgtc tattttttgt gataacgcca ttgaggcagc tcttgaagca
      961 caacaacctc atatgtcaat tgcttacttt ttgttaggtg actatcagat gtttgttgtg
     1021 acaaatacga ctaaaaaaaa ggtagacatt aacaagatct ttgaagaagg ctattcttca
     1081 aaaggttcag aacgtggtat cggcctttct aatgcgcagc gtattctcaa aaagtatcct
     1141 tatttatcct tgcggactaa aagttttgat aaggaattta gccaaacctt gaccatgcca
     1201 aaggaggagg tggatagatg atggttatat acagtatggc tttattctat gctaccatct
     1261 ttattaacag ctgggtcatt tttgcaaagg tttctgctat taaactaagc tggaaacggg
     1321 ttagcattat aggcattagt tttgtaattg ctaatatgat atttgacaaa gtcattttga
     1381 ttgaccaact gttctttatt attgttagtt tgttaagtgc tccgaaaaag aaattatttg
     1441 agcatatgtt taatggtttc tttactatct tgattgtcga attactgttt cgcgtcatcg
     1501 gatctttctt tttaccagct gtcttaggat ttagcattgg tcaaattaat aataacctta
     1561 agcttttaga gttatgttat ctttttgtat taccaatatt ttatctgttt agctacattt
     1621 tcagtattga cttaagctta ataaggttta tcagtgaaga taagatgaaa aagtgggtat
     1681 tttggatgaa tactgccatg ttttcttact actttttcgc ccattttttg gtaactgtgc
     1741 aaagcggatt tcttgcacta tatttccagt accggtctat tttagtattt atttatttag
     1801 ctatttttat ttgggttatt gtgaaattag atcgatttgc taaagatcag ctgtcacaaa
     1861 aattaacaca agcacaaaat gaacgtattg cgtatcttga aaattacaat caatcgattg
     1921 agcaacttta ccgagaaatc agaacagtta aacatgattc tgaaaatatt ttaattagtc
     1981 taaaagatag tatcgatagt ggtgatatag atctgattac tagagtttat gacacggtta
     2041 ttcagcaatc ggcaacttct atgatgcgca caaattatga aatatcttct ttagacaata
     2101 tcaaagaggc tgtgattcgc agtatcatga attccaaatt gcttgaagct cagtatttgg
     2161 gtattgaact ttatattgag attccagatg ttatcgatca tttacctatt aagttaatcg
     2221 acttaattgt actttttact ggactagtgg acaatgctat tgagacagcc aaaggcagta
     2281 gacgtccttt tttatctatt gcatacttta aacaagataa caagcaatta tttattattg
     2341 aaaatagcac aaaaaccaat cgtgtggata tagccaaacg ctttgatgct cagcagcaga
     2401 actcagccca ctttttgact gttttagata gttaccccca aataacgctt tcgactaaaa
     2461 gtgaccatta tcgattaaga caattgttag aaatgagata aagtatgatg aacattttta
     2521 ttcttgagga tgattttatc cagcagacac gcatagaatc tatcgtagta ggcatcttaa
     2581 aagagactag aataccatgt aatcagcttg aagtgttctc gactcctcaa aaattatttg
     2641 agagtatcca agaaagagga gaccatcagc tgtatttttt agatattgaa attggtgaat
     2701 atactcgttg tgggcttgaa ttagctgcag ctattcgaca aaaagatcct aatgccgtta
     2761 ttgtgtttgt caccactcat tcagaatttg ctcctatttc ttttaaatat aaggtatccg
     2821 ctctagattt tattgataaa gcaggtggtc aaaaacagtt taaagaacaa atcgaagaat
     2881 gtatccgata tacttacgat atgatgtcta gtcgtgagtc aaaggacatg tttttatttg
     2941 agactccaca gacaagatta aaattgccct ataaggatat tttatatttt gctacagcca
     3001 caactcctca caaagtttgt ttatggactc agacagaaag attagagttt tatggtaatc
     3061 tatctgagat tcaagcagta gcaccgaaac tttttttgtg ccatagatcc taccttgtga
     3121 atctagataa ggttgttcga attgataaga gtaagcagct cctttatttc gaaaatggtg
     3181 attcttgtat ggtatcacgg ttaaaaatga aatgtctgtt tgaacggtgg gaggcccttc
     3241 gctaaagtta agagagcaat aacatttcag gacgaaaaag caacatttca ggaaatgatg
     3301 ctcttattga agtatttttc gctataataa ttaatgtaaa taaagattta cgaagtcatg
     3361 agtttatcga gcaatcaatc cccaattcaa tcaaaatatt gttttctctc tctccataaa
     3421 caaatagtat ttttgatatg atggctcggc agacaaaatg acgatgtcag ttgtctttgt
     3481 ttggatatca cattagctgg ttgtaaaatc agtattaaag cccggcttgc cgggtttttt
     3541 gataggtaaa tacagggata attagctgac gtcttgtgga ttctgacttt ttatggttgt
     3601 tttctgaagg ttattccttt gtcgaaagaa gaactatact acgaaaagat ggagtgacaa
     3661 gaggcagttg aagcacaaaa actagaaata gtacagaaag aggcttaatc tgaaagggtt
     3721 agggcttttt aattttttag gggatgtggt ataatggtaa taatttagat tatggagtta
     3781 aattttgaag aagacctatc gtgtcaagcg tgagaaagat ttccaagcca tatttaagga
     3841 tggaaaaagt acagcaaatc gaaaatttgt catttatcat ttaaatagag gccaagacca
     3901 cttccgtgtg ggtatttctg tcggtaaaaa aataggaaat gcagtcacca gaaatgcagt
     3961 caaacgaaag atacgtcatg ttatcatggc attagggcat cagctgaagt cagaggattt
     4021 cgtggtgatt gcccgtaagg gtgtcgagtc tttggagtat caagagcttc aacaaaattt
     4081 acatcatgtt ttaaagttag cacaattgct tgagaaaggt tttgagagtg aagaaaaaca
     4141 ttaaaattgc aagaatagtc cccttagttc tattattagt ggcctgtggt cgtggtgagg
     4201 taacggcaca atcatctagt ggttgggacc agttggttta cttatttgcc agagcaattc
     4261 aatggctttc ctttgatggt tcaattggtg ttggcattat tctttttacc cttactatcc
     4321 gtctcatgct catgcctttg tttaatatgc aaatcaaatc aagccagaag atgcaggaca
     4381 tccaacctga gcttagagaa ttgcaaaaga aatacgctgg taaagacaca caaacgcgga
     4441 tgaagttggc tgaagaaagt caagcccttt acaaaaaata tggggtcaac ccttatgcta
     4501 gcctcttacc cctcttaatt cagatgccag ttatgattgc cctattccaa gccttgacac
     4561 gggtatcttt cttaaaaaca gggactttct tgtgggtgga attggcacaa catgatcatt
     4621 tgtacctttt accagttttg gcagctgtct tcactttctt gtccacttgg ttgaccaatc
     4681 tagcggctaa agaaaaaaat gtcatgatga ctgttatgat ttatgtgatg ccgctaatga
     4741 tctttttcat gggctttaac ttggctagtg gagtagtgct ctattggacg gtctcaaacg
     4801 cctttcaagt ggtacaacta ctgttattaa acaatccttt taagattatt gccgaaagac
     4861 agcgtcttgc caatgaagaa aaagaacgcc gtcttcgtga acgtcgtgct cgtaaaaaag
     4921 ccatgaagag aaaataggag tttaggttat ggtattattt acaggaaaaa cagtcgaaga
     4981 agctatcgaa acaggacttc aagagttagg gttatcacgc cttaaagcgc atatcaaagt
     5041 catttcaaaa gagaaaaaag gtttcctcgg gttcggtaaa aagccagccc aagttgatat
     5101 cgaaggtatc agcgacaaga cggtttataa ggctgataaa aaagccactc gaggtgttcc
     5161 agaggatatt aatcaaaaaa atgctcccgc ggtcaattct gctgatgtag aacctgaaga
     5221 aataaaagcc actcaaaagt tagaagcaga agataccaaa gtggttcctc tcatgtctga
     5281 agatagtcca gcgcaaaccc ctgctaatct tgctgagaca gtcactgaaa gaaaagcaca
     5341 acaaccgtca atcccagtcg aggaatcaga agtgccccaa gatgctggta atgatggttt
     5401 cagcaaagat attgaaaaag ctgctcagga agtgtccgat tatgtgacta aaatcatcta
     5461 tgaaatggac attgaagcta ccgttgaaac cagcaacaat cgtcgccaaa tcaatttgca
     5521 gattgaaacg ccagaggcgg gacgtgtgat tggttaccat ggtaaggtct tgaaatcatt
     5581 gcagttgtta gctcaaaatt tcttgcacga ccgctattca aaaaattttt cagtatcctt
     5641 gaatgtccat gattatgtgg aacatcgaac agaaaccttg attgacttta cgcaaaaagt
     5701 tgccaaacgc gtcttggaat ctggtcaaga ttacaccatg gatcccatga gtaacagtga
     5761 gcgtaaaacc gttcataaaa ccgtttcatc cattgaaggg gttgatagtt attctgaagg
     5821 caatgaccct aaccgctatg tggtagtcag tcttcaacgt tagatcctaa aaagaatctt
     5881 tggtagttag tttagctatc gaagattttt tgtatgacca gcttaaccgt ttgttttact
     5941 gatctcattg atcattattg ctattgataa ggtttcaacg gtaaggcaaa ggaacatgtc
     6001 aaagaagaga cactttatga aatgagcggc ctcttaaatg agagagaaac tgttgaaaaa
     6061 cgaaaataaa tcttgacagt agaccctctt taagatagaa tattatagta tgtttagtaa
     6121 ctgatatcac aaatagccgg ctaaacgaat acaaaatcta tgaggaggta ttcactgtga
     6181 aacgtactta tcaaccaagt aaaatccgtc gtcaacgtaa acacggattc cgtcaccgta
     6241 tgtcaactaa aaacggacgt cgcgtgcttg ctgcacgtcg tcgtaaagga cgtaaagtat
     6301 tatcagctta attgcaaaaa atacaactaa agtaaaccaa gagtgctcga ggctcttggt
     6361 tttttaacgt tttctaatcg gaaatttgca atgtataagt atggtgtcaa ctccttacct
     6421 tcttatttgt tgtatgaaag agcattccaa ataatagaaa ataatataaa aaatgaaagt
     6481 agattctaaa agtctcattg ctattgaaag cgctttattt tttgttataa taactttgtg
     6541 aacgttattt ttattaaaac taagaaacac aaggagtaga aactatgcct gataaaccaa
     6601 ccaaagaaaa gctcatggag cagttaaagg gtgggattat cgtttcttgt caggctttgc
     6661 caggtgagcc tctttattca gagactgggg gtattatgcc actgctggca aaagctgctc
     6721 aagaagctgg tgctgtcggc atcagagcta attcggttag ggatattaaa gaaattcaag
     6781 ccataacaga cctaccaatt atcggtatca tcaaaaaaga ttacccgccg caagagcctt
     6841 ttattacggc aacgatgacc gaagtggatc aattagctgc cttaaatatt gcagtgattg
     6901 ctatggattg taccaagcgt gaccgacatg atggcttgga tattgctagc tttattcgac
     6961 aagttaagga aaagtacccc aatcagcttt tgatggcaga tattagtacc tttgatgaag
     7021 gactagtggc ccatcaggct ggcattgatt ttgttggaac caccttgtca ggctacaccc
     7081 cgtatagccg tcaggaagca ggtcctgatg tggcattgat agaagctctt ttcaaagcag
     7141 gcattgctgt cattgccgaa ggaaagattc attccccaga agaagccaag aaaattaatg
     7201 acttaggtgt ggcaggtatt gttgttggag gggccatcac aaggcctaag gaaattgcag
     7261 agcgttttat cgaagcgctc aaatcctaat agtggttgtc aaactagaaa cacacatcaa
     7321 aaaaaggaga ttttacgatg aacatgaaga agttagcttc attagcgatg cttggtgcat
     7381 ctgttttagg actagcagct tgtggcggaa agagccagaa agaggctggt gcaagtaaat
     7441 ctgatactgc taagacagaa atcacatggt gggctttccc ggtcttcacg caagaaaaag
     7501 ctgaagatgg tgtgggaacc tatgagaaga aattgattgc tgcctttgag aaagctaatc
     7561 cagaaatcaa ggtaaaattg gaaaccattg attttacatc tggtcctgag aaaatcacaa
     7621 ctgctattga agctggaaca gctcctgatg tcctctttga tgctccaggt cgtatcattc
     7681 aatatggtaa aaatgggaaa ttggctgact tgaatgactt gttcacagaa gagtttacca
     7741 aagatgttaa caatgacaaa ttgattcaag cctcaaaagc aggagataca gcttacatgt
     7801 atcctatcag ctctgctcca ttctacatgg cgttgaataa aaaaatgctc aaagatgctg
     7861 gcgtccttga ccttgtgaaa gagggttgga caacagatga ttttgaaaaa gtcttgaaag
     7921 cattgaagga caaaggatat aacccaggtt ctttctttgc taatggtcaa ggtggtgacc
     7981 aaggaccacg tgccttcttt gctaaccttt atagtagtca tattaccgat gacaaagtaa
     8041 ccaaatacac cacagatgat gctaactcta ttaaagccat gaccaagatt tccaattgga
     8101 ttaaagatgg tttaatgatg aatggctctc agtacgatgg ttcagctgat attcaaaact
     8161 ttgcgaatgg ccaaacttcc tttaccattc tttgggcgcc tgcacaacca ggcatccaag
     8221 ctaaattgtt agaagctagt aaagtggatt accttgaaat cccattccca tcagatgatg
     8281 gcaaaccaga actagaatac ctcgtaaatg gttttgcggt ctttaataac aaagatgaac
     8341 aaaaagtcgc tgcctctaag acatttatcc aatttatcgc tgatgataaa gaatggggac
     8401 ctaaaaatgt tgttcgtaca ggtgctttcc ctgtaagaac ttcttatggg gatctttaca
     8461 aagacaaacg aatggaaaaa attgctgaat ggacaaaatt ctactcacca tactataaca
     8521 cgattgatgg gtttgctgaa atgagaactc tttggttccc aatggttcaa gccgtctcta
     8581 atggtgatga aaagccagaa gatgctttga aagccttcac tgaaaaagca aacaagacaa
     8641 tcaaaaaaac acaataagtg ttcagaacaa gtggcttctt ttcacggcac atgtcaaatg
     8701 gtcactggaa aggagccttt ccttttttta ggagagatta atagaaaaag aggtatttca
     8761 agtgaacgtc aataaattaa aaatgcgaga gacgctcatc tcatacgctt tcttagctcc
     8821 ggtcttggtt ttctttgtga tttttgtctt gataccgatg attatgggct ttgtgacgag
     8881 ctttttcaat tattccatga cagagtttac ctttgttggt tttgccaatt atgctaggat
     8941 gtttcaagat cctatcttta tgaaatctct tatcaatacg ttgattatcg taattggttc
     9001 agtaccagtg gtggttttct tttctctctt tgtggctgct aagacttatg acaaaaatgt
     9061 ggtggcacgt tcgttttatc gggcagtttt ctttctacct gttgttaccg gaagtgttgc
     9121 cgtaacggtg gtttggaaat ggatttatga tccgatgtcg gggattttga attatgtctt
     9181 aaaatatgct catgtgattg aacaaaatat cagttggctg ggagataagc actgggcttt
     9241 gttggctatt attgttattt tattaaccac atctgttggg cagcctatta ttctatatat
     9301 tgcagctatg ggaaatattg ataactcttt ggtagaagct gcgcgtgtag acggcgccac
     9361 agaatttcag gtgttttgga atatcaagtg gccaagtctc ctgccaacaa ccttatatat
     9421 tgctgtcata acaaccatta attccttcca atgttttgct ttgattcaac tcttgacgtc
     9481 tggagggcca aattattcaa ccagtaccct catgtactat ttgtatgaaa aagcctttaa
     9541 gctctctgaa tacggctatg ccaacacaat gggtgtattc ttggcagtca tgattgcaat
     9601 catcagcttt gctcaattta aaatattagg taatgatgtg gaatattagg aggatattaa
     9661 gatgacgaaa aagaaactaa ccgcatcaga tatcttaacc actgtgatgc tatgtgtttt
     9721 gaccattttg tttatttttc cattttattg gattatgaca ggagcattta aggctcaagc
     9781 cgataccatt atgattccac cgcaatggtg gccaaaggcg cctactattg aaaattttaa
     9841 ggccttggta gtgcaaaatc cagccttaaa atggttgtgg aatagtgtct ttatttcggt
     9901 ggcgaccatg ttcttggttt gcggaacctc ctcgttggct ggctatgctt tggccaaaaa
     9961 gcggttttat ggacagcggc ttttgttttc gatttttatt gctgctatgg ccttgccaaa
    10021 acaggttgtt ctagtgcctc ttgtgcggat tgttaatttt atgggaatcc atgacacttt
    10081 ggcggctgtt attttgcctc ttgtgggctg gccatttggt gttttcctta tgaaacagtt
    10141 ctcagaaaat attccaaccg agttattgga atccgccaag attgatggat gtggcgaaat
    10201 tcgtaccttt ttcaatgtgg ctttccctat tgtcaaacca ggctttgcag cgctagcaat
    10261 ttttaccttt atcaatactt ggaatgatta ttttatgcag ttggtgatgt taacctctcg
    10321 ggaaaattta acgatttcac ttggggttgc cactatgcaa gctgaaatgg ctactaatta
    10381 tggcttgatt atggcagggg ctgccatggc tgcagtgcct attgtgacgg tatttcttgt
    10441 cttccaaaaa tcatttaccc aaggcattac tatgggtgct gtgaaaggtt agactaaata
    10501 aagcgtgtca ctaagcttaa tctataggtt gcctcttaat ttaggtgcat agatagataa
    10561 gtcaaaaggg aagcaagtca tgtgttaagc gtttaagagg agagttagct tttatttgta
    10621 agcgtatttc tagaaaggaa gttgtgattt gacatcgaaa aaacagggac ttttacactc
    10681 tctcttcaag cttgatagta aatggatgag ggctagcgca gcgctgtttg atttgctcgt
    10741 gtttaatctc ttatttgtct tgtcctgcct tccccttcta acgattgggg ttgctaaaat
    10801 ggctttgtat gcgagtttgt tggattggcg agaaggtcaa gttagtcagc ttgttactac
    10861 ttatagctct cattttaaac attattttaa aagtgggatg cgcctaggtc ttattgagct
    10921 tttgataatg agtatttgtg tcttagatct tttcttaatt cgaaaccaat caggcctagt
    10981 ttttcaaggg tttaaagtac tttgcattgc tgttttattt ttggtagtta tactcttttt
    11041 gtacgcttat cctcaggccg tcaaaagaga cctttcccta tctacgctgt ttaagcgaag
    11101 ctttttatta gcaggactct tttttccatg gagttttgct ttcttggctt ttatttgtct
    11161 cacgatattt agcctacaat tgtctctgtt aacgttattt ggaggcgtgt ctttgctagc
    11221 tatcatcggc atcagtagtc taacttatct ctacttgatt atcatggaat ctcttcttag
    11281 gcgattccca cttaataatg acattgaata aaggacatta ctatgacaga tcttacgaaa
    11341 taccaaggca ttattcctgc cttttacgct tgttacgatg accaggggaa tatcagccct
    11401 gagcgtgtgc gtgccttgac ccagtactat attgacaaag gggttcaggg gctttacatt
    11461 aatggctctt ctggagagtg tatttaccag agcgtttttg accgtcaatt ggtgttagaa
    11521 aatgtcatgg cagtcgctaa gggcaagctg acgattatta atcacgttgc ctgcaacaac
    11581 actaaagata gtattgaatt ggcagctcat tcagaacgtt tgggcgtgga tgccattgca
    11641 gctattccgc ctatttattt ccggttacca gaatatgcgg ttgctgatta ttggaatgct
    11701 atttcttcgg ctgctcctca cacagatttt atcatctata atattcctca actggcaggg
    11761 gttgccttga cgccaagcct ttataagact atgttggcta ataagcgcgt gattggcgtt
    11821 aaaaattcat ctatgccagt gcaggatatt caaaccttct gtgcgattgg tggggacgat
    11881 catattgttt ttaatggccc agatgaacaa tttctaggag ggcgcttgat gggtgctgct
    11941 gcaggcattg gtggcaccta cggggctatg ccagaattat ttttaaggct gaaccaactt
    12001 attgctgaca aggatttaga aaaggccaag gcccttcaat atactattaa tgagattatc
    12061 ggtgtccttg tttctgctca cggcaatatg tatggggtta ttaaagaagt acttcgtatt
    12121 aatgagggtc ttgatattgg ttctgttcga tcacctttag ctgagttggt cgaggaagat
    12181 agggttattt gccaacgggc tgctgctctg attaatcagg ctaaggaaac cttttgataa
    12241 gatgcgaggg gataacaaat gaagcattat ttagcaattg atattggtgg tactgctatc
    12301 aagtatggct tgatttctga aacaggagac ctgttggaaa aagaggaaat ggccactgag
    12361 gcttataaag gtggtccctc tattttggaa aaggtcaagg gcttggtaaa gacctatcaa
    12421 gaccaaatgg atctggctgg agtggccatt tcttctgcag gaatggttaa tcctgatgag
    12481 ggagaaatct tttatgccgg ccctcagatt cctaactatg ctggaactca gtttaaaaaa
    12541 gagattgaag agactttcgg gttaccgtgt gaagtggaaa acgatgttaa ctgtgctggt
    12601 ctagccgaag ctatttctgg tagtgccaag gattatcctg tggctctttg cttgacaatt
    12661 gggacgggaa tcggtggctg tttattgttt aattcccaag tttttcatgg tagtagtcat
    12721 tcagcttgcg aagttggtta ccttcatttg tcagatggtc agtttcagga tctggcatct
    12781 accacagctc ttgttcaaga ggtggcctta gcttatggcg atgacattag ccaatgggat
    12841 ggtcgtcgta tttttgagca ggctaaagcg ggtgatgcga tttgtatagc agccattagt
    12901 aagcaggtgg attacttagg acaaggtatt gctaatatct gctacgttgt taacccaaat
    12961 gtggtggtgc ttggtggcgg tatcatggcg caaaaagact atttagctga taaattaaag
    13021 acagctcttg atagctactt ggtttctagt cttgccaaga aaactcagct gaagtttgct
    13081 agtcatggaa ataatgctgg tatattagga gcttactacc attttaaaca aaagaatgag
    13141 cgatcttgtt catttataac tcttgagaaa atcgaaaata atttagcgag tagctaatct
    13201 tagtggctgt gagtctatat gacgtgaaga gttcttgttt aagtatactg ggggaggtga
    13261 atgacgaccc tttaaaaaga aaacaacgcc ctttttgcta ttgtagtcta aaggtcgtta
    13321 ttttctttgt caataatagt gtttttgaag attttttctt tgtgcggttt gtcgatcgct
    13381 aaaacgtaag cataaataat gtccatcata ataagaagag gaaattgtgg tgacacacga
    13441 ttcccataat gtagttggtg ggttgatgat acgggaataa tatctaagga gtcgtcaaat
    13501 tcggtttgat tatctgttgt taagaggaca gttttggccc ctcgctgact agcttgatga
    13561 agtgctgtga tgacagagtt tgttttgcca gataaagaaa aaccaaaaac aaggcaatta
    13621 tcattgacga ggctatttgc ccaagtaaaa ccatcagtgt ctgaataagc atcacagatt
    13681 aaaccaagtc gcataaagcg tagcttcatt tctcttgcta ctagtccaga agatcctttg
    13741 ccaaagaaat agactcgctc agaagaatcg atcagcttgg ctaagttcag taatttttcc
    13801 tcattaacga gctcataggt tttattaata agagcgtcgt agtccatcag cacgcgcttt
    13861 gtcaattcta agtgaataga ttggaaagtc tcttgtgatt cttgaagact gtgaaggtaa
    13921 tcaaaggcaa aagcacgata accagtaaaa ccacattttt ttgcaaatcg agtcaaggcg
    13981 gcttgagaga tgtgtagtcg cttaactatc tcggatgcgg tcaactcttg aggtgtcaga
    14041 tcggttgtaa taaaaaaatg agcaatccct ttttctaaac tggtcatatg ttccaatgat
    14101 gcttcgattt tggtaattag gcggtggttt ttaggcatag caaagcacac tccttacagt
    14161 ttaactacct actagtatag catagatcgc taacagaagg tagcgaccct ttttgtgata
    14221 cagatttgtt caacccactt tatttgagta ggttgtagta tttttgaggg cttatgtagg
    14281 agccgactat taaaggtcat ttgtcaaaaa agtagaaaat agacaagtgt cagctagcaa
    14341 agggttttac gatcaaccta aacggcagaa gttctcacca agttttctga tgtggttaag
    14401 atctttatgt tataataaca tcatgacaaa taaagtagaa aaactaacta tttttgatac
    14461 ccatacgcac ctgaatgtag cagaatttca aggacacgaa actgaagaat tgactttggc
    14521 tcaagaaatg ggcgtggcct accataatgt ggttggcttt gaccaggcca ctattagtgg
    14581 ggcactaact ctagccaata aatatgccaa tatttatgca actattgggt ggcatccaac
    14641 agaagcaggt tcttattcag aggctgttga ggaggctatc gtctctcaat tatcacattc
    14701 caaagtgatt gccttaggtg aaattggctt agactattat tggatggagg accccaaaga
    14761 ggtgcaaata gaggttttta agcgccaaat gcaattagca aaagaccatg atttgccttt
    14821 tgtggtgcac acccgcgatg ccttggaaga tacctatgag gtcatcaagg cagctggtgt
    14881 tggccctcgt ggaggcatca tgcattctta ctcagggtca ttagagatgg ctgaacgttt
    14941 cattgaactt ggcatgatga tttccttttc aggtgtggtc actttcaaaa aggctcttga
    15001 tattcaagag gcagcgcagc acttgccttt ggataagatt ttggtggaaa cagatgcccc
    15061 ttatcttaca ccagttccaa aacgtggcaa acaaaatcat acagcttata cccgttatgt
    15121 ggttgataaa atcgcagagc tacgggggat gactgtggaa gaagttgcaa aagcaacgac
    15181 tgctaatgca aagagggtat tcaagcttga ctgaaaaaat taatattcaa gaggttcttg
    15241 ttgtagaagg caaggacgac acggctaacc tacgccgttt ttacgaggtg gatacctatg
    15301 agactagagg ctctgctatc actgaagaag atttagaacg aatcaatcgc ctaaacgatt
    15361 tgcgtggtgt tatcgtttta acagacccag attataatgg tgagcgcatt cgtaagctca
    15421 ttatggctgc tgtgccaaca gctcgtcatg cctttttaaa ccgaaacgag gcggttccga
    15481 gttctaagag taagggtcgt tcattagggg ttgaacatgc cagatttgag gatctccaaa
    15541 aggcacttgc tcatgtcact cagcaatatg atgatgagtc ttattttgac attcgtcaaa
    15601 cagacctgat tcgtctaggg cttttaatgg cgtcagatag ccgtaaacgc agggaatact
    15661 tgggtgaaaa gctccgcatc ggttatgcca acggcaaaca actgctcaag cgcctcgaat
    15721 tatttggcat taccctagca gaagtggaag aagtgatgga gacatatgag gagtaaagag
    15781 tacaataaaa tataacagcc ccattaactg aatggcgtgt cctggcaaaa aagtttaggc
    15841 tagtggttca aatgccaaaa agaaagtgat cataaaaaga agagaatata tgagaattgc
    15901 agattatagc gtgaccaagg ctgtccttga tcgtcacgga tttaccttta agaaatcttt
    15961 tggtcagaac tttttgaccg ataccaatat tttacagaaa atcgttgaca cggctgagat
    16021 tgatcagaat gtcaatgtta tcgagattgg tcctggaatt ggggctttga cggaattttt
    16081 ggcagaaaat gctgctgaag tgatggcttt tgagattgat gaccgcttgg tgccgatttt
    16141 ggcagatacc ttgcgcgatt ttgataatgt gcaagtggtc aatcaagata ttttgaaagc
    16201 tgatttgcag acccaaatca agcagtttaa aaatcctgat ttgcctatca aggtggtggc
    16261 taacctacct tactacatca caacgcctat tctcatgcac ttgattgaga gcaaaattcc
    16321 attccaagag tttgtagtca tgatgcagcg tgaagtggca gatcgtattt ctgcggagcc
    16381 aaacactaaa gcttatggtt ccttgtccat tgcggtacag tattacatga ctgccaaggt
    16441 tgccttcatt gtgccgcgta ccgtctttgt acctgcacca aatgtcgata gtgctatcct
    16501 caaaatggtg cgtcgcgacc agcctttgat tgaggttgag gacgaagatt tctttttcag
    16561 agtgtctcgt ctcagttttg ttcatcgtcg caagaccctg tggaataact tgactagcca
    16621 ttttggcaag tctgaagaca tcaaggctaa acttgaaaaa ggtctggcac tagcagatat
    16681 caagccttct attcgtggtg aggccttgtc gattcaagat tttggcaaat tagctgatgc
    16741 tttaaaagaa gttggattat aaaagctttc ccccggtgac aataaaaaaa ttcccttgta
    16801 gacaaaacag agatgttcag ttcaggggga tttttcttat tgaagctggc cgttaaggca
    16861 tcgtagtgca gagctagttt ttttaatacc tcggaaaaaa aggggcaatt aacaagagcg
    16921 tgactatagc ggtacggtta gactcttttt cgtaaatagc aaaggtcttc ttaaaattca
    16981 tatcgtttcc tacctcaaaa aatcatcagt gatctgattt ttagttgata aaatattcta
    17041 tttttaaaaa gcagtgacct aaatgatagc cagtggtcta gagaatcttg acagtttttt
    17101 aaggcgagtg aaacttagac tcaaatgctg atttgggagc ggttttctga tataatagag
    17161 ttattatagt agggcaaagg agcacagttg caaggtaaaa tcattaaatc tctggctggt
    17221 ttttattacg tggagtcaga aggacaggta tatcagacgc gagcgcgtgg taattttagg
    17281 aagcgcggtg agacacctta tgttggagat atcgtggatt tctcagcaga ggacaactct
    17341 gaaggttata ttttagcgat tcatcctcgt aaaaacagtt tggtaagacc tcctatcgtc
    17401 aatattgacc aagcagtggt tattatgtct gctaaagaac cagaatttaa cagcaatctt
    17461 ctagaccgct ttctcatctt gcttgaacac aaagccattc atccagtggt ttatatctct
    17521 aagatggact tattagatag tcccgaggag attaaggcta ttggtcgaca gtatcaggca
    17581 atcggttacg attttgtgac cagtcttgag gaattgttgc ctttgctagc tgataaaatt
    17641 accgtcttta tgggacaaac aggtgttggg aaatcaaccc ttctcaaccg aatcgccccc
    17701 gaattggctc ttgaaacagg agaaatctca gatagtctag ggcgtggtcg tcacaccacc
    17761 cgagctgtga gtttttacaa tacccatggc ggaaaaatcg cggatacgcc aggtttctca
    17821 tcattagact atgatattgc taatgctgaa gatttgaacg aggcttttcc agagctgcgc
    17881 cggctgagcc atgagtgtaa gtttagatcc tgtacccata cccacgaacc caagtgtgct
    17941 gtcaaggcag cgcttgaaac gggagagttg tggccagttc gttatgagca ttacctgcaa
    18001 tttctcagtg aaattgaaaa ccgtcgtgac acatataaaa aagttatcaa aagaaagtag
    18061 gtctactcca tgtcaactct aaaaattgcc ccatctattc tagcagctga ttacgctaat
    18121 tttgcttctg agctagctcg tattgaagaa acagatgccg aatacgttca cattgacatt
    18181 atggatggtc aatttgttcc taacatcagt tttggtgcag atgttgtggc aagtatgaga
    18241 aagcacagta aattggtttt tgattgtcat ttaatggtgg ttgatcctga acgctatgtt
    18301 gaggcttttg cacaagctgg tgctgatatc atgaccattc acacagaaag tactcgtcat
    18361 attcatagag cccttcaaaa aatcaaggca gctggcatga aagcaggggt agtgattaat
    18421 ccaggaactc ctgcaacagc cctagaacct ttgttagact tagttgacca agtcctcatt
    18481 atgacagtca acccaggttt tgggggtcag gcctttatcc cagaatgttt agaaaaagtg
    18541 gctaccgtag ccaaatggcg agatgaaaaa gaattgtcct ttgacattga ggttgatggc
    18601 ggagtagaca ataagaccat tcgtgcttgt tatgaagcag gggccaatgt ctttgtggca
    18661 ggttcttacc ttttcaaggc cagtgaccta gtgagccaag tccaaacctt aaggacggcc
    18721 ttgaatgtct aaagttgctc tttttgcggg aggagatctc tcttatatca gccgtgattt
    18781 tgattatttt gtcggtattg atagagggag cctattttta ttagaaaatg ggcttccttt
    18841 gaatatggct gttggtgatt ttgattcggt ttcccaaaaa gcgtttacgg atataaaaga
    18901 gaaggcagag ctttttatta ctgctcatcc agaaaaaaat gatacggata cggaattagc
    18961 ccttaaagag gtctttgctc gttttcctga ggcggaagtg accatttttg gggcttttgg
    19021 gggtcgaatg gaccacctct tatccaatat tttcttgcct agcgaccctg gtattgctcc
    19081 ctttatggca caaatcgcct tgcgtgacca acaaaatatg attacctatc gcccggcagg
    19141 tcagcacctt atccatcaag aagaagggat gacctatgtg gcatttatag cagaaggaga
    19201 agctgacttg accattactg gtgctaaatt tgagttgacg caagataatt ttttcaaaaa
    19261 gaaaatctat tccagtaatg cctttatcca tcaacctatt acagttagtt tgccaagtgg
    19321 ctacttgatt attattcaaa gtaaagattg gagttagtat ggaccttatc ttgttccttt
    19381 tggtcttggt tctcttaggt ttaggggctt atctgttgtt caaagtcaac ggccttcaac
    19441 atcagcttgc ccaaacccta gaaggcaacg cggataattt gtctgatcaa atgacctacc
    19501 agttggatac agctaacaag caacaattgt tagagctaac acagctgatg aaccgacaac
    19561 aagcagacct ttaccaacaa ttaacagata ttcgtgacgt cttgcaccgt agtttgtctg
    19621 atagtaggga ccggtctgac aaacgcttag aaaaaattaa ccagcaggtc aaccaatcgc
    19681 tcaaaaatat gcaagaatct aacgaaaaac gtttggagaa aatgcgccag atcgttgaag
    19741 aaaaattgga agaaacctta aaaaatcgtc tgcacgcctc tttcgattct gtatccaagc
    19801 aactagaaag tgtcaataaa ggcttgggag aaatgcgtag cgtggctcaa gatgtgggta
    19861 ctttaaataa ggttttgtcc aataccaaaa cacgaggcat tttaggcgaa cttcaactag
    19921 gccaaatcat tgaggatatc atgacatcaa gccagtacga aagagaattt gtaacggtta
    19981 gtggttctag tgaacgcgta gaatatgcga ttaagctccc aggaaatggt caaggcggct
    20041 atatttacct accgattgac tcaaaattcc ctcttgaaga ttattaccga ttagaagatg
    20101 cttacgaagt tggtgataaa ctggccatcg aggctagccg aaaagcactt ctggcagcta
    20161 tcaaacgctt tgccaaagac attcataaaa agtacttgaa ccccccagag acgaccaatt
    20221 tcggagttat gttcttacca acagaaggtc tttattcaga agtggtcaga aatgcagctt
    20281 tctttgatag ccttcgtcgg gaagaaaata ttgtggttgc aggtccttcg accctgtctg
    20341 ctttgctcaa ttccttatct gttggtttca agacccttaa tatccaaaaa aatgctgatg
    20401 acatcagtaa aattttgggc aatgtcaagt tagaattcga taaatttggc ggcctgcttg
    20461 ccaaggctca aaagcaaatg aatacagcta ataatacgct ggatcagctc atttcaacaa
    20521 ggacaaatgc cattgttcga gccttgaata ccgttgaaac ttatcaagac caagcaacaa
    20581 aatctctctt gaacatgccc ttattagaag aggaaaataa tgaaaattaa tcaaatgaaa
    20641 aaagaccagc tttttgaagg gttctacctc attaagtcgg cagaggttcg taagacaagg
    20701 gctggtaaag attttattag ccttaccttt caggatgaca ctggcgagat ttcaggaaat
    20761 ctgtgggatg cccaacctta taacgttgaa gaatttaccg ctggtaaagt cgtgtttatg
    20821 aaagggcgac gcgaggttta taatggtacc cctcaggtta atcaaatcac cttgcgaaat
    20881 gttcgcccag gggagcccaa tgatccaaag gacttcaaag aaaaggcacc tgtcagtgtt
    20941 gaagaagtac gtgactattt ggaacagatg ctctttaaaa ttgaaaatgc aacgtggcaa
    21001 cgaattgtaa gggctcttta tcgtaagtat gataaagaat tttatactta tcctgcagct
    21061 aaaacaaacc accatgcttt tgagtcaggc ctcgcctacc atactgctac gatggtcaga
    21121 ctagcagata gcattggaga tatctaccct gacttaaata agagtctact gtttgcaggt
    21181 attatgttac atgacttagc taaagtcata gaattaacag gaccagttaa tacagaatac
    21241 accgttcgtg gtaacttgat tgggcatatt tctctcatta acgaagaaat tacaaaagta
    21301 atttcagaac tacaaatcga tgataccaaa gaagaagtca ttgtccttag gcacgtgatt
    21361 ttaagccatc acgggcaatt agaatacggt agtccggtac gtccacgcat catggaagct
    21421 gaaattattc atatgattga caatattgat gctaatatga tgatgatgac aacagctctt
    21481 agccgtgtta gcgagggcga gatgaccaat cggatttttg ccatggataa tcggtctttt
    21541 tacaagccaa actattgatc agtgtttctc aagtaatagt tcctttgctt ttgtgatgat
    21601 ttttgatgtg cttaacactt atgatggttt cataagtgtt tttggctctt gttatggtta
    21661 ttaagtagaa tgtcctttca gtaggaatgt tgattagttt atcttagatt gaattcttgc
    21721 atga
//
LOCUS       DAUCWU010000020        20749 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUCWU010000020 DAUCWU010000000
VERSION     DAUCWU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.020"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            239..1840
                     /locus_tag="VGY24_000737"
     CDS             239..1840
                     /locus_tag="VGY24_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4283739.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAMGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSS
                     SKSHSDKEVQKPEKPDNSVKQATSS"
     gene            2028..3275
                     /gene="mrp"
                     /locus_tag="VGY24_000738"
     CDS             2028..3275
                     /gene="mrp"
                     /locus_tag="VGY24_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Mrp"
                     /protein_id="HEQ4283740.1"
                     /translation="MSTRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKADLS
                     TREHPRVTEAREKALEEVIANMSLDELQQTLAGSYRKNRELEKTIEKKDGEASFLSKV
                     LDETAKKYRESSDKYKQEIGQLKAAAEAEAQTALDALNNKNKQISDLTNENAQLKEAI
                     EGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEEIAKLQSEAAT
                     LENLLGSAKHELTDLQAKLDTATAEKAKLESQVTTLENLLGSAKRELTDLQAKLDAAN
                     AEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAKQAEE
                     LAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEETTNPF
                     FTAAALTVIASAGVLALKRKEEN"
     gene            3499..4659
                     /locus_tag="VGY24_000739"
     CDS             3499..4659
                     /locus_tag="VGY24_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ4283741.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKATEV
                     KAAGQSAPKGTNVSADLYNSLWDENKTLREKQEEYITKIQNEETKNKELDKKNKELDS
                     RVTDLIDVIEHDDQELKRKDRMYEAFLKQSKDQVKDLNAEKDTLAEKAKKLEEDKQIS
                     DASRQGLSRDLEASRAAKKELEANHQKLEAEHQKLKEDKQISDASRQGLSRDLEASRE
                     AKKKVEADLAALTAEHQKLKEEKQISDASRQGLSRDLEASREAKKKVEADLAEANSKL
                     QALEKLNKELEEGKKLSEKEKAELQARLEAEAKALKEQLAKQAEELAKLKGNQTPNAK
                     VAPQANRSRSAMTQQKRTLPSTGETANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            4868..5938
                     /locus_tag="VGY24_000740"
     CDS             4868..5938
                     /locus_tag="VGY24_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ4283742.1"
                     /translation="MTRQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRADDA
                     TTQGTNFLHKYVKQLEEEREKLEEEKVELLDKVDQLEQQKQQEQKEHKERQEKLEQLE
                     HKYQVETDKRYQEQQKKYQQYQQEQEEKQKNLEQLERKYQREVDKRYQEQQQKQQQLE
                     AENKKLTEANQVSEASRKGLSNDLEASRAAKKELEAKHQKLAEEHQVSETSRKGLSRD
                     LEASREANKKVTSELTQAKAQLSALEESKKLSEKEKAELQAKLDAQGKALKEQLAKQT
                     EELAKLRAEKAAGSKTPATKPANKERSGRAAQTATRPSQNKGMRSQLPSTGEAANPFF
                     TAAAATVMVSAGMLALKRKEEN"
     gene            6273..9848
                     /gene="scpA/B"
                     /locus_tag="VGY24_000741"
     CDS             6273..9848
                     /gene="scpA/B"
                     /locus_tag="VGY24_000741"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ4283743.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETKIPQTPGDAEETVADDANDLAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQAKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     SKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGQVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSDQAPDKKPETKPEQDGSDQAPDQKPEAKPE
                     QDGSGQTPDKKPEKDSSGQTPDKKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALAT
                     KASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
     gene            9945..11213
                     /gene="fbpA"
                     /locus_tag="VGY24_000742"
     CDS             9945..11213
                     /gene="fbpA"
                     /locus_tag="VGY24_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibronectin-binding protein FbpA"
                     /protein_id="HEQ4283744.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDNVQSI
                     SSTQENTASAPKKWHHIDDNGFIPLGISLETAKKDFKKEVEESRLSKEQKEKYHQKID
                     AETNKDELLSTYHSEYSKDIKNLPASTELTTQPVEAPVQETQAPASDSMVTGDSTSVT
                     TDSPEETPSSESPVAPETPALSEAPAQPAESEEPSVAASSEETPSPSTPAAPETPEEP
                     AAPAPSPESEEPSVAASSEETPSPSTPAAPETPALSEAPAQPAESEESSVAATTSPSP
                     STPAAPEIPALSEAPAQPAESEESSVAATTSPSPSTPAESETQTPPALTKDSDKPSSA
                     AEKPAASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGE
                     KAQPLFIATMTLMSLFGSLLVTKRQKETKK"
     gene            11449..12369
                     /gene="lmb"
                     /locus_tag="VGY24_000743"
     CDS             11449..12369
                     /gene="lmb"
                     /locus_tag="VGY24_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ4283745.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKNAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPNGNKTYLENLRANLEVLYQQLK"
     gene            12382..14853
                     /locus_tag="VGY24_000744"
     CDS             12382..14853
                     /locus_tag="VGY24_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ4283746.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GRGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELSAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            15021..15221
                     /locus_tag="VGY24_000745"
     CDS             15021..15221
                     /locus_tag="VGY24_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ4283747.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(15303..15929)
                     /locus_tag="VGY24_000746"
     CDS             complement(15303..15929)
                     /locus_tag="VGY24_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283748.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFDMLKKLCDACYMIEENRTQLFKHT"
     gene            complement(15913..16716)
                     /locus_tag="VGY24_000747"
     CDS             complement(15913..16716)
                     /locus_tag="VGY24_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283749.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIRQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(16728..17549)
                     /locus_tag="VGY24_000748"
     CDS             complement(16728..17549)
                     /locus_tag="VGY24_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283750.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(17546..18523)
                     /locus_tag="VGY24_000749"
     CDS             complement(17546..18523)
                     /locus_tag="VGY24_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283751.1"
                     /translation="MKRTTIIIIWKIIRCITLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKDVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFTYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(18636..20264)
                     /locus_tag="VGY24_000750"
     CDS             complement(18636..20264)
                     /locus_tag="VGY24_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4283752.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDITSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            20443..20667
                     /locus_tag="VGY24_000751"
     CDS             20443..20667
                     /locus_tag="VGY24_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283753.1"
                     /translation="MTYFRLKKELNWLLRSKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFFEVVRAAATFSTAKNICFGLGLLRSQK"
BASE COUNT         7319 a         4098 c         4055 g         5277 t
ORIGIN      
        1 aatgtcgcta aaaaaataga tagaagagtc taaaatttca gttttaggtc acgttttatg
       61 aataggaatc aggatgatga ccttaagttg tgatttgcgc gtcattgacc gctttccttg
      121 ttttaatgaa agaatttgaa agatatttta tggtatactt ttccttaata tggttcatac
      181 ggacttgata aatctattta caaaaataat aaaaaattaa gataaggaca tgaagttaat
      241 gcatgtaagt aaattattta ctagccaaca atggagagaa ttgaaactga tctcatattt
      301 aacagaaaac tccaatgcta tgggagttaa agataaggag cttagcaagg ctctcaatat
      361 ttccatgtta acattacagt catgcctcac taatatgcag tttatgaaag aagtaggtgg
      421 aattacctac aaagacggct atattaacat ttggtatcac cagtgttgtg ggctacaaga
      481 ggtctatcaa aaagctcttc gagaatcgcc ttctttgaaa ttactggagc ttttgttttt
      541 cagagacttt agttccttag aagagttagc tgaagagctg tttgtcagct tatctaccct
      601 caaacgcctc atcaaaaaaa ccaacactta cttgtctcat acctttgcta ttagtatcgt
      661 gacaagtcca gtgcaggtgt caggagatga gaggcaaatc cgcctgtttt acctcaagta
      721 tttttcagaa gcctataaaa tctcagaatg gccatttggg gacattttaa acctgaaaaa
      781 ctgtgaacgc ctattgagct tattgatcaa agaagtagat gtcaaagttc attttacact
      841 atttcagcac ttaaaaatct taagtggtgt caacttgatc cgttactaca aggggtattc
      901 ttgcagttat aacaataaaa aaaccagtca ccgtttttct caactcatcc aacattcctc
      961 agaaatccag gatctttctc gcttgttcta cctcaaattt gggttacatc tcgacgagta
     1021 taccatagct gaaatgtttt ctaaccatct taacgacaag ctagaaattg gctgtgcttt
     1081 tgagattatc aatcaagacc caacatcagg aggcagacaa gtaaccaact ggatccatct
     1141 attagatgag atggaaatca agctcaatct cagcatcacc aataaatacg aagttgccgt
     1201 aaccctccat aacgcttcgg tgttaaacga agaagacatt acagccaatt atttgctgtt
     1261 tgattataaa aagagttacc tgaactttta ccaaaaagaa caccctcgta tctatgaggc
     1321 ctttgtgaca agtgtcgaga agctgatgca ggcagataat gctcaagtta gcaaagaatt
     1381 gatcaatcag ctcacttatt gtttctttat tacctgggaa aatagtttct taaaagtaaa
     1441 tcaaaaagat gaaaaagtac gactcctagt aatagagaga agttacaata gtgtcggtaa
     1501 ttttttgaaa aagtacatcg gtgagttttt cagcattact aattttgatg agttagattg
     1561 tttgacaatt gatctagtag agattgaaaa acagtacgat gttatcgtga cagatgttat
     1621 ggtgggtaaa agcgaagagt tagagatttt ctttttctac aagatgatcc cagaagctat
     1681 tattgataga ttaaacgaat ttttgaacgt tagtttcaca gataacaacg ttatggtcaa
     1741 aaacctcgaa gccccttcct cttcaaaatc tcatagtgac aaagaggtac aaaaaccaga
     1801 aaagccagac aattcagtta aacaagcaac atcatcatag gatttcagac gtcatggtaa
     1861 gaaaaaaaga atcatttaca gcccatagag gtaaggtcaa aagctgaaaa cagctcaaaa
     1921 aaactgacct ttaccttttg gcttttttta tttagaataa ttttattgga gagatgctta
     1981 ataatttaag cacaattctt agaaagttaa gaaataagga gtaaacaatg tctacaagaa
     2041 atccaaacaa acactactca ctgagaaagt taaaaacagg tactgcatca gtagcagtag
     2101 ctttgacagt tttggggact ggattagcaa acacaactga tgtaaaggct gacttaagta
     2161 ctcgggaaca tcctagagta acagaagcaa gagaaaaagc tttagaagaa gttatagcaa
     2221 atatgtcttt agatgaactc cagcaaactc tagcaggatc ttaccgtaag aacagagagc
     2281 tggaaaagac aatcgagaaa aaggatggcg aggcttcttt cctttctaaa gttttagacg
     2341 aaactgcaaa aaaatataga gaatctagcg acaagtataa acaagagatt ggacaactaa
     2401 aagcagcagc agaagcagaa gctcaaacag cattagatgc actaaataac aaaaacaagc
     2461 aaatctcaga cttaaccaac gaaaacgcac agttaaaaga agccattgaa ggttatgtgc
     2521 aaactatcca aaacgctagt cgtgaaatcg cagcaaaaca acaagaactt gcagctgcaa
     2581 aaagccagtt agaggcaaaa aatgctgaga ttgaggcatt gaaacaacaa gatgcctcta
     2641 agactgagga aattgctaaa ttgcaatcag aagcagcaac tttagaaaac ctcctaggtt
     2701 cagctaagca tgagttgact gacttgcaag ctaagctaga tacagcaact gctgaaaaag
     2761 caaaactaga atcacaagta acaaccttag aaaaccttct aggttcagct aagcgtgaat
     2821 tgactgatct gcaagctaag ctagatgcag ctaacgctga aaaagaaaag ctccaatcac
     2881 aagcagcagc cctagaaaaa caactagaag caactaaaaa agagttagct gatctacagg
     2941 ctaaattagc agcaaccaac caagaaaaag aaaagttaga agctgaagca aaagctctta
     3001 aagagcaatt agcaaaacaa gctgaagagc ttgctaaact aaaagcagat aaagcttcag
     3061 gagctcaaaa accagatact aaacctggca ataaagaggt tccaacaaga ccatcacaaa
     3121 caagaacaaa cactaataaa gctcctatgg cgcaaacaaa gagacaatta ccgtcaacag
     3181 gcgaagaaac aaccaaccca ttcttcactg cagcggcatt gacagtgatc gcatctgcag
     3241 gcgtacttgc cctaaaacgc aaggaagaaa actaagccca acccacacta tcttttctag
     3301 cccaagaaaa aacaaaaaaa gaggaagacc attcctcttt ttttgaacgg ttgaaaagca
     3361 aaaaggtcaa aaaggcacta aagtctcaaa aacctggtct ttacctttta ccgcttattc
     3421 tttagaatag aattattaga gagaagtctt agaaaaatga ggctaattcc ctaaagatga
     3481 aaaaataagg agcaaataat ggctagaaaa gatacgaata aacagtattc gcttagaaaa
     3541 ttaaaaacag gtacagcatc cgtagcggtc gctgtggctg ttttaggagc aggctttgca
     3601 aaccaaacag aagttaaggc aactgaagtt aaggctgcgg ggcaaagcgc tcctaaaggt
     3661 acaaacgtga gcgcagacct atataattcg ctatgggatg aaaataaaac tcttagagaa
     3721 aaacaagaag agtatataac aaaaattcaa aatgaagaga caaaaaataa agagttagat
     3781 aagaaaaata aagaattaga ctctcgagtt acagatctca tagacgtaat tgaacatgac
     3841 gatcaagaac ttaaacgcaa agatcgcatg tacgaagctt tcttaaaaca gtctaaagat
     3901 caggttaaag acctgaatgc tgaaaaagat acactggcag aaaaagccaa aaaacttgaa
     3961 gaagacaaac aaatctcaga cgcaagccgt caaggtctaa gccgtgacct tgaagcgtct
     4021 cgtgcagcta aaaaagagct tgaagcaaat caccaaaaac tagaggctga gcaccaaaaa
     4081 cttaaagaag acaaacaaat ctcagacgca agccgtcaag gcctaagccg tgaccttgaa
     4141 gcgtctcgtg aagctaagaa aaaagtagaa gcagacctag ctgctcttac tgctgagcac
     4201 caaaaactca aagaggaaaa acaaatctca gacgcaagcc gtcaaggcct aagccgtgac
     4261 cttgaagcgt ctcgcgaagc taagaaaaaa gtagaagcag acttagcaga agcaaatagc
     4321 aaacttcaag cccttgaaaa actaaacaaa gagcttgaag aaggtaagaa attatcagaa
     4381 aaagaaaaag ctgagttaca agcaagacta gaagctgaag caaaagctct taaagagcaa
     4441 ttggctaaac aagctgaaga acttgccaaa ctaaaaggca accaaacacc aaacgctaaa
     4501 gtagccccac aagctaaccg ttcaagatca gcaatgacgc aacaaaagag aacgttaccg
     4561 tcaacaggcg aaacagctaa cccattcttt acagcagcag ctgcaacagt gatggtgtct
     4621 gcaggtatgc ttgccctaaa acgcaaagaa gaaaactaag ctattagact gatgctaaag
     4681 ctaagagaga atcaaatgat tctctctttt tgagtggcta agtaactaac aatctcagtt
     4741 agaccaaaaa atgggaatag ctcaaaaagc tggcctttac tccttttgat taaccgtata
     4801 taataaaaac attaggaaaa taatagcact attaagttgc tttttcaata aaatcaagga
     4861 gtagataatg actagacaac aaaccaagaa aaattattca ctacggaaac taaaaaccgg
     4921 tacggcttca gtagccgttg ctttgaccgt tttgggcgca ggttttgcaa accaaacaga
     4981 agtaagagct gatgatgcta ctacgcaggg gactaatttt ttacataagt acgttaaaca
     5041 actggaagaa gaaagagaaa aattagaaga ggaaaaagta gaattacttg ataaagtaga
     5101 tcaattagaa caacaaaaac agcaagaaca aaaagaacac aaagaacgac aagaaaagtt
     5161 agaacaatta gagcataaat accaagtaga aacagataag cgttatcaag aacaacaaaa
     5221 gaaatatcag caataccaac aagaacaaga agaaaaacag aaaaatctag aacaattaga
     5281 acgtaaatat caacgagaag tagataaacg ttaccaagaa caacaacaaa aacaacaaca
     5341 attagaagca gaaaacaaaa aactaacaga agccaatcag gtttcagaag ctagtcgtaa
     5401 aggtctaagt aacgacttag aagcttctcg tgcagctaaa aaagaactag aagctaagca
     5461 ccaaaaatta gcagaagaac atcaagtttc agagactagc cgtaagggtc taagccgtga
     5521 ccttgaagca tcacgtgaag ctaataagaa ggttacatct gagttaacac aagcaaaagc
     5581 tcaactctca gcgcttgaag aaagtaagaa actatcagaa aaagaaaaag ctgagttaca
     5641 agcaaaacta gatgcacaag gaaaagccct caaagaacaa ttagcaaaac aaactgaaga
     5701 gcttgcaaaa ctaagagctg aaaaagcggc aggttcaaaa acacctgcta ccaaaccagc
     5761 taataaagaa agatcaggta gagctgctca aacagctaca agacctagcc aaaataaagg
     5821 aatgagatca caattaccgt caacaggcga agcagccaac ccattcttta cagcagcagc
     5881 tgcaacagtg atggtatctg caggtatgct tgctctaaaa cgcaaagaag aaaactaagc
     5941 ctttagaact tggtttttgt aacggtgcaa tagacaaaag caagcaaggc caaaaactga
     6001 gaaagtctta aaaagctggc ctttacccct caaaattaat gttttataat aaagatgtta
     6061 gtaatataat tgataaatga gatacattta atcattatga caaaagcaag aaaaatagct
     6121 gtatcatatg caaataaccc ctgtttgctc tttaaaaaag acgttatcct tatttctcca
     6181 cgcacagatg gacagctagg agagaatcgt ttgattctct cttttcttaa tggtcataaa
     6241 gacaaagtct ctcatcatga aaggacgaca cattgcgtaa aaaacaaaaa ttaccatttg
     6301 ataaacttgc cattgcgctc atgtctacga gcatcttgct caatgcacaa tcagatatta
     6361 aagcaaatac tgtgacagaa gacactcctg ctaccgaaca agccgtagaa accccacaac
     6421 caacagcggt ttctgaggaa gcaccatcat catcaaagga aaccaaaatc ccacaaactc
     6481 ctggtgatgc agaagaaaca gtagcagatg acgctaatga tctagcccct caagctcctg
     6541 ctaaaactgc tgatacacca gcaacctcaa aagcgactat tagggatttg aacgaccctt
     6601 ctcaggtcaa aaccctgcag gaaaaagcag gcaagggagc tgggactgtt gttgcagtga
     6661 ttgatgctgg ttttgataaa aatcatgaag cgtggcgctt aacagacaaa actaaagcac
     6721 gttaccaatc aaaagaagat cttgaaaaag ctaaaaaaga gcacggtatt acctatggcg
     6781 agtgggtcaa tgataaggtt gcttattacc acgattatag taaagatggt aaaaccgctg
     6841 tcgatcaaga gcacggcaca cacgtgtcag ggatcttgtc aggaaatgct ccatctgaaa
     6901 cgaaagaacc ttaccgccta gaaggtgcga tgcctgaggc tcaattgctt ttgatgcgtg
     6961 tcgaaattgt aaatggacta gcagactatg ctcgtaacta cgctcaagct atcagagatg
     7021 ctgtcaactt gggagctaag gtgattaata tgagctttgg taatgctgca ctagcttacg
     7081 ccaaccttcc agacgaaacc aaaaaagcct ttgactatgc caaatcaaaa ggtgttagca
     7141 ttgtgacctc agctggtaat gatagtagct ttgggggcaa aacccgtcta cctctagcag
     7201 atcatcctga ttatggggtg gttgggacgc ctgcagcggc agactcaaca ttgacagttg
     7261 cttcttacag cccagataaa cagctcactg aaactgctac ggtcaaaaca gacgatcatc
     7321 aagctaaaga aatgcctgtt ctttcaacaa accgttttga gccaaacaag gcttacgact
     7381 atgcttatgc taatcgtggg atgaaagaag atgattttaa ggatgtcaaa ggcaaaattg
     7441 cccttattga acgtggtgat attgatttca aagataagat tgcaaacgct aaaaaagctg
     7501 gtgctgtagg ggtcttgatc tatgacaatc aagacaaggg cttcccgatt gaattgccaa
     7561 atgttgatca gatgcctgcg gcctttatca gtcgaaaaga cggtctctta ttaaaagaca
     7621 attctaaaaa aaccatcacc ttcaatgcga cacctaaggt attgccaaca gcaagtggca
     7681 ccaaactaag ccgcttctca agctggggtt tgacagctga cggcaatatt aagccagata
     7741 ttgcagcacc cggccaagat attttgtcat cagtggctaa caacaagtat gccaaacttt
     7801 ctggaactag tatgtctgcg ccattggtag cgggtatcat gggactattg caaaagcaat
     7861 atgagacaca gtatcctgat atgacaccat cagagcgtct tgatttagct aaaaaagtat
     7921 tgatgagctc agcaactgcc ttatatgatg aagatgaaaa agcttatttt tctcctcgcc
     7981 aacaaggagc aggagcagtc gatgctaaaa aagcttcagc agcaacgatg tatgtgacag
     8041 ataaggacaa tacctcaagc aaggttcacc tgaacaatgt ttctgataaa tttgaagtaa
     8101 cagtaacagt tcacaacaaa tctgataaac ctcaagagtt gtattaccaa gcaactgttc
     8161 aaacagataa agtagatgga aaacactttg ccttggctcc taaagcattg tatgagacat
     8221 catggcaaaa aatcacaatt ccagccaata gcagcaaaca agtcaccgtt ccaatcgatg
     8281 ctagtcgatt tagcaaggac ttgcttgccc aaatgaaaaa tggctatttc ttagaaggtt
     8341 ttgttcgttt caaacaagat cctacaaaag aagagcttat gagcattcca tatattggtt
     8401 tccgaggtga ttttggtaat ctgtcagcct tagaaaaacc aatctatgat agcaaagacg
     8461 gtagcagcta ctatcatgaa gcaaatagtg atgccaaaga ccaattagat ggtgatggat
     8521 tacagtttta cgctctgaaa aacaacttta cagcacttac cacagagtct aacccatgga
     8581 cgattattaa ggctgtcaaa gaaggggttg aaaacataga ggatatcgaa tcttcagaga
     8641 tcacagaaac catttttgca ggtacttttg caaaacaaga cgatgatagt cactactata
     8701 tccaccgtca cgctaatggc aagccatatg ctgcgatctc tccaaatggg gacggtaaca
     8761 gagattatgt ccaattccaa ggtactttct tgcgtaatgc taaaaacctt gtggctgaag
     8821 tcttggacaa agaaggaaat gttgtttgga caagtgaggt aaccgagcaa gttgttaaaa
     8881 actacaacaa tgacttggca agcacacttg gttcaacccg ttttgaaaaa acgcgttggg
     8941 acggtaaaga taaagacggc caagttgttg ctaacggaac ctacacctat cgtgttcgct
     9001 acactccaat tagctcaggt gcaaaagaac aacacactga ttttgatgtg attgtagaca
     9061 atacgacacc tgaagtcgca acatcggcaa cattctcaac agaagatcgt cgtttgacac
     9121 ttgcatctaa accaaaaacc agccaaccgg tttaccgtga gcgtattgct tacacttata
     9181 tggatgagga tctgccaaca acagagtata tttctccaaa tgaagatggt acctttactc
     9241 ttcctgaaga ggctgaaaca atggaaggcg ctactgttcc attgaaaatg tcagacttta
     9301 cttatgttgt tgaagatatg gctggtaaca tcacttatac accagtgact aagctattgg
     9361 agggccactc taataagcca gaacaagacg gttcagatca agcaccagac aaaaaaccag
     9421 aaactaagcc agaacaagac ggttcagatc aagcaccaga caaaaaacca gaaactaaac
     9481 cagaacaaga cggttcagat caagcaccag accaaaaacc agaagctaaa ccagaacaag
     9541 acggttcagg tcaaacacca gacaaaaaac cagaaaaaga tagttcaggt caaacaccag
     9601 acaaaaaacc agaaaaagat agttcaggtc aaacaccagg taaaactcct caaaaaggtc
     9661 aaccttctcg tactctagag aaacgatctt ctaagcgtgc tttagctaca aaagcatcaa
     9721 caagagatca gttaccaacg actaatgaca aggatacaaa tcgtttacat ctccttaagt
     9781 tagttatgac cactttcttc ttgggattag tagctcatat ctttaaaaca aaacgcactg
     9841 aagattagta caactgatca gatggctact attgatgatg agttagatca gtcttctttt
     9901 caaaagaagg catagcagta taaaaataga tatgtgagga caatatgcgt agagcagaaa
     9961 ataacaaaca cagccgctat tccattcgca aactgagcgt tggggtaacg agtatagcaa
    10021 ttgcgagtct ctttttagga aaggttgcct atgccgtaga taacgtgcag tcaatctctt
    10081 cgactcaaga aaacacagct agtgcaccca aaaaatggca tcatattgat gataatggct
    10141 ttattccttt aggtataagc ttagaaactg ccaaaaagga ttttaaaaaa gaagtagaag
    10201 aatcacgttt gtctaaagaa caaaaagaaa agtatcatca aaaaattgat gctgaaacaa
    10261 acaaagacga actattgtca acgtatcata gtgagtactc taaagatatt aagaatcttc
    10321 cagcgtctac tgagcttact actcagccag ttgaggcacc cgtgcaggag acacaggcac
    10381 cagcttcaga ttcgatggtg acaggtgatt caacatcagt tacgactgat tctcctgagg
    10441 aaaccccatc ttcggaaagt ccagtggccc cagaaacccc agctttatct gaggctccag
    10501 ctcaaccagc tgagagtgag gaaccttcag tagcagcatc ttctgaggaa accccatctc
    10561 catcaactcc agcggcccca gaaactcctg aagaaccagc agctccagct ccatcacctg
    10621 agagtgagga accttcagta gcagcatctt ctgaggaaac cccatctcca tcaactccag
    10681 cggccccaga aaccccagct ttatctgagg ctccagctca accagctgag agtgaagaat
    10741 cttcagtagc agctacgaca agcccgtctc catcaactcc agcggcccca gaaatcccag
    10801 ctttatctga ggctccagct caaccagctg agagtgaaga atcttcagta gcagctacga
    10861 caagcccgtc tccatcaact ccagctgaat cagagactca gacgccacca gctcttacta
    10921 aagactctga taagccatct tcagcagctg aaaaaccagc agcctcttca cttgtttcag
    10981 aacaaaccgt tcaacaacca acttcaaaga gatcttctga taaaaaagaa gagcaagaac
    11041 agtcttactc tccaaatcgc tcattgtcaa gacaggttag ggcccatgag tcaggtaagt
    11101 acttgccttc aacaggtgaa aaagcacagc cactctttat agctactatg actttgatgt
    11161 ctctatttgg cagtctttta gtcacaaaac gccaaaaaga aactaaaaaa tagtattact
    11221 taaactgata agagcttatt taatagagag aaccaaattg gttctctcta tttttttaga
    11281 tgtgctgcct catcaaaaag aagttaggac aaatagctgt caacaattgc cttttgctta
    11341 tctcttcagt tgctgtgata aaagatggta gttgccattg acaaagcatt gataaaggag
    11401 taaaattaac tggttaataa ctggttaaat tataattgag gaggtactat gaaaaaaggt
    11461 ttttttctca tggctatggt cgtgagttta gtaatgatag cagggtgtga taagtcagca
    11521 aaccccaaac agcctacgca aggcatgtca gttgtaacca gcttttaccc aatgtatgcg
    11581 atgacaaaag aagtatctgg agacctcaat gatgtgagga tgatccaatc aggtgcaggc
    11641 attcattcct ttgaaccgtc tgtaaatgat gtggcagcta tttatgacgc ggatttgttt
    11701 gtttaccatt cacatacctt agaagcttgg gcaagggatc tagaccctaa tttaaaaaaa
    11761 tcaaaggttg atgtgtttga agcgtcaaaa cctttgacac tagatagagt caaagggcta
    11821 gaagatatgg aagtcacaca aggcattgac cctgcgacac tttatgaccc acatacctgg
    11881 acggatcccg ttttagctgg tgaggaagct gttaatatcg ctaaagagct aggacgtttg
    11941 gatcctaaac acaaagacag ttacactaaa aatgctaagg ctttcaaaaa agaagcagag
    12001 caactaactg aagaatacac tcaaaaattt aaaaaggtgc gctcaaaaac attcgtgacg
    12061 cagcacacgg cattttctta tctggctaaa cgattcggct tgaaacaact tggtatctcg
    12121 ggtatttctc cagagcaaga gccctctcct cgccaattga aagaaattca agactttgtc
    12181 aaagaataca acgtcaagac tatttttgca gaagacaacg tcaatcccaa aattgctcat
    12241 gctattgcga aatcaacagg agctaaagta aagacattaa gtccccttga agctgctcca
    12301 aacggaaaca agacatatct agaaaatctt agagcaaatt tggaagtgct ctatcaacag
    12361 ttgaagtaaa ggagattatt agtgaagaaa acatatggtt atatcggctc agttgccgcc
    12421 attttactag ctactcatat tggaagttac caacttggta agcatcatat gggtctagca
    12481 acaaaggaca atcagattgc ctatattgat gacagcaaag gtaaggcaaa agcccctaaa
    12541 acaaacaaaa cgatggatca aatcagtgct gaagaaggca tctctgctga acagatcgta
    12601 gtcaaaatta ctgaccaagg ctatgtgacc tcacacggtg accattatca tttttacaat
    12661 gggaaagttc cttatgatgc gattattagt gaagagttgt tgatgacgga tcctaattac
    12721 cgttttaaac aatcagacgt tatcaatgaa atcttagacg gttacgttat taaagtcaat
    12781 ggcaactatt atgtttacct caagccaggt agcaagcgca aaaacattcg aaccaaacaa
    12841 caaattgctg agcaagtagc caaaggaact aaagaagcta aagaaaaagg tttagctcaa
    12901 gtggcccatc tcagtaaaga agaagttgcg gcagtcaatg aagcaaaaag acaaggacgc
    12961 tatactacag acgatggcta tatttttagt ccgacagata tcattgatga tttaggagat
    13021 gcttatttag tacctcatgg taatcactat cattatattc ctaaaaagga tttgtctcca
    13081 agtgagctag ctgctgcaca agcctactgg agtcaaaaac aaggtcgagg tgctagaccg
    13141 tctgattacc gcccgacacc agccccaggt cgtaggaaag ccccaattcc tgatgtgacg
    13201 cctaaccctg gacgaggtca tcagccagat aacggtggct atcatccagc gcctcctagg
    13261 ccaaatgatg cgtcacaaaa caaacatcaa agagatgagt ttaaaggaaa aacctttaag
    13321 gaacttttag atcaactaca ccgtcttgat ttgaaatacc gtcatgtgga agaagatggg
    13381 ttgatttttg aaccgactca agtgatcaaa tcaaacgctt ttgggtatgt ggtgcctcat
    13441 ggagatcatt atcatattat cccaagaagt cagttatcac ctcttgaaat ggaattagca
    13501 gatcgatact tagctggcca aactgaggac aatgactcag gttcagatca ctcaaaacca
    13561 tcagataaag aagtgacaca tacctttctt ggtcatcgca tcaaagctta cggaaaaggc
    13621 ttagatggta aaccatatga tacgagtgat gcttatgttt ttagtaaaga atccattcat
    13681 tcagtggata aatcaggagt tacagctaaa cacggagatc atttccacta tataggattt
    13741 ggagaacttg aacaatatga gttggatgag gtcgctaact gggtgaaagc aaaaggtcaa
    13801 gctgatgagc tttctgctgc tttggatcag gaacaaggca aagaaaaacc actctttgac
    13861 actaaaaaag taagtcgcaa agtaacaaaa gatggtaaag tgggctatat tatgccaaaa
    13921 gatggcaagg attatttcta tgctcgtgat caacttgatt tgactcagat tgcctttgcc
    13981 gaacaagaac taatgcttaa agataagaaa cattaccgtt atgacattgt tgacacaggt
    14041 attgagccac gacttgctgt agatgtgtca agtctgccga tgcatgctgg taatgctact
    14101 tacgatactg gaagttcgtt tgttatccct catattgatc atatccatgt cgttccgtat
    14161 tcatggttga cgcgtgatca gattgcaaca atcaagtatg tgatgcaaca ccccgaagtt
    14221 cgtccggatg tatggtctaa gccagggcat gaagagtcag gttcggtcat tccaaatgtt
    14281 acgcctcttg ataaacgtgc tggtatgcca aactggcaaa ttatccattc tgctgaagaa
    14341 gttcaaaaag ccctagcaga aggtcgtttt gcaacaccag acggctatat tttcgatcca
    14401 cgagatgttt tggccaaaga aacttttgta tggaaagatg gctcctttag catcccaaga
    14461 gcagatggca gttcattgag aaccattaat aaatctgatc tatcccaagc tgagtggcaa
    14521 caagctcaag agttattggc aaagaaaaac gctggtgatg ctactgatac ggataaaccc
    14581 aaagaaaagc aacaagcaga taagagcaat gaaaaccaac agccaagtga agccagtaaa
    14641 gaagaagaaa aagaatcaga tgactttata gacagtttac cagactatgg tctagataga
    14701 gcaaccctag aagatcatat caatcaatta gcacaaaaag ctaatatcga tcctaagtat
    14761 ctcattttcc aaccagaagg tgtccaattt tataataaaa atggtgaatt ggtaacttat
    14821 gatatcaaga cgcttcaaca aataaaccct taaccaaaag aagatctcat tgttaaagca
    14881 ctgctttgtc aaagcaagtt acggtgattt tgaagtcatt ctatgtaacg agtagtgata
    14941 aaagttggat aatagcggtt ttcttttgca aagaaatggt atccatgtta gaataataaa
    15001 aaaagaggag gattctaatc atgtcagaag aaaaattaaa atcaaaaatt gagcaagcat
    15061 ctggtggcct aaaagaaggt gctggtaagt tgacaggtga taaagagcta gaagcaaaag
    15121 gatttgttga aaaaacaatt gctaaaggca aagaacttgc agacgatgca aaagaagcgg
    15181 ttgaaggtgc tgtagatgcc gttaaggaaa aattaaaata attaaaaaaa ccagctgtaa
    15241 gctggttttt ttaatggtgt gattttgtta gaaaaagact caataggtaa taaccatagg
    15301 tgttaggtat gcttgaacag ttgagttcga ttctcttcaa tcatatagca cgcgtcacag
    15361 agttttttta acatatcaaa atcatgacta ataacgatga gacctaaatt tctatccttt
    15421 acaatctcca acaggctttt ccatacacta gcttgtgtaa tgctatccaa catagtagtc
    15481 atttcatctg caataaggta tttcgtctct ggatgtaatg aacgcacaat cgaaaagcgt
    15541 tgcaattccc ctcccgagag ttcactagga cgacgcttta gccatttttc ttgaatgcca
    15601 aaagcatctc gcaaatcttg acttggatag taggcttctt ccaaactttt tttcataggc
    15661 cataaaggat tcatggtttg ttcaggatgt tgttggataa gttgtacagg tctaaatgct
    15721 ttgttaggca aatgactgcc atcaactaat acttcaccag atttaggctg caaaaaaccg
    15781 gcaagcactc tggataaact cgtttttcca catccacttt gtccaaatat ccctaagatt
    15841 tgaccaggtg ctacctctaa atcaatctcc ttaaaaagcc attgatcttt tttatgataa
    15901 aagccaagct ttttagcctc taaggtcatg agtaactcct ttcaaaaagt cttgctgagg
    15961 gagtgtgcgc cataaacgtc tcgcaaattc tgtttgtaat tgctccccac ctccgctaaa
    16021 aaagctagct ggggctgttt caatagcttt tccctcttta aaaatagtaa tacgatcagc
    16081 aatttgacta gctgctacaa tatcatgagt gataaatatg acgcttattc ctttatctgc
    16141 aaaggagcgt agttggtcta aaaccatttg cagagcatct ggatgtaatc caggggtggg
    16201 ctcatccgca ataatcaaag aaaccgtatc actaatacac gttgtaaaca aaacacgtcg
    16261 gagcattccg ccagaaagtt ggaaaggata caagtcacca tcactttctt ttaaaccaaa
    16321 ctgttgaaac aatccttctt gagtagcctt agcattttct gagataccta agcgcacctg
    16381 atgtttgact ttcatagatg gatctaaata attaacggat tgtggaatca acgtcatttc
    16441 ttttcctcgc aactgtctga tgcgtttaga agttagtgat tgaccacgat aaatcatatc
    16501 tcctgttaca gctgcatttt taggaagaat atccataata gcatgcgcta ataaactctt
    16561 gcctgaacca ctagcaccta taatagctaa taactcacct tttttaactt ctaaattcag
    16621 cgcttgtatc ggtgtggatt gaaatggttt taaaaatctt ccgtattgag tgaaagtgat
    16681 ggagaggtct ttaatgctta ataatgtttc tgtcatatct ctacctccta aaaatgatcc
    16741 gtttgagggt aaaagagttt ctttaaagat tctccgatag tatcaaaggc attgacaacc
    16801 aaaataagat aaaggcctgg aaaaatcacc aaccaccaat tgccaagaga gatatgctta
    16861 gctgcttctg acaaaatgat accaaccgaa ggttgttcgg cagaaaggcc aaatcctaag
    16921 aaagtcatgg atgcttcatg caagatgacg tgcggaaata agaggataaa cccaatgaaa
    16981 atttgagaag caatcaaagg caagatatga tgcctcacaa tataataagg cgtttttccc
    17041 atgcttttag agagttggac aaaggcttta ttctttagat catagacttc attgcggata
    17101 agtcttgcta gagagggcca atgggtaaca gccgttgcaa tgataacccc ttgagcccct
    17161 ttcccaacaa caaaagaaat gagaatcata aaaatcaaat gaggcatacc aataaacaaa
    17221 tcaaccaacc aggctattat tttatcaata aggctatttc ctagacctgc aagcactcca
    17281 aaaacggtcg caagaaagac tcccataagg gcacctaata agccgacttg taaagagaaa
    17341 taaagccctt taatcgttct gacaaacata tccctaccta aaccatctgt cccaaaaaga
    17401 tggtttaagg aaggggcgag gttgcgtaaa gctgcattgg tttccaaagg cgtcctatag
    17461 aaataaaggt ttaaggctag aatactaaga atgagagaaa tggcgatacc cagttgccat
    17521 aaaaccatcg ttcgacgttt caatatcata cttttctcct taactgtgga ttgatgatgc
    17581 tatttaaaat atccgcaata agattgcccg caaaaacaaa taatgtccct atcatcacaa
    17641 tagctagaag gagcggtgta tcacttttaa gtcctgcttc agtgagggta gaccctaagc
    17701 caggatatga gaaaacttgc tcagcaagaa cagatcctcc aaacaattct ccgaaatagg
    17761 aaaaatgcag tgtaatagct ggtacgatag catttctaag gcaatgatgt ttaaaaattt
    17821 gccactgtgt ttccccacgc gctctggcaa ataagacata ttcactagaa agcaccgaca
    17881 tcattttagt tctcgtatga agggtgacat tagcaatacc tagaatactt aacgtgaaaa
    17941 caggtaacat aaggtgctta atccgatcag ctaacgtaat atcttgactt aaagtcccaa
    18001 tcggggaaga aataccaatc gggaaccacc ccagctggac agaaaagatt aataaaaaaa
    18061 tgaggccaat ccaaaacgtt ggtactgata tctgaaggta agtaaaccac ctgacaactc
    18121 ggtcaagtaa tttcccttga tggaaagctg ataacgttcc taagataaat ccaataagac
    18181 ccgataagat ccaagagagt cccataagta tgaaagaagc acctgcccgt gatctaataa
    18241 tatcactaac aggttgccga taaacgagcg aggtccctaa atgtccctgt atcacatctt
    18301 tcaaccaaat aaaatattgg actagagctg gcttatccaa gccatagtgg tgagcaatcg
    18361 ctttgtactg agcaggggtt agtgatgtgt cataattgac acttgccatg acgggatcta
    18421 ctggagattg ttttaacaaa acgaaggtca aaacagatac cccaaaaata agtgtgatac
    18481 atctgatgat tttccagatg ataataatgg tagtacgttt cacaatgaaa tctccttaat
    18541 cgataagaaa aggagccgag gaaacgatca gcacagtttt gtgtatcttg ttttctcaga
    18601 ctccaaactt aaagtcgctg tattaactaa acgcgttact tagttgattc atcccaagtc
    18661 cactcggcaa tgttagtcaa taatgaccaa tcatgaccat gactgtggac gccttgttta
    18721 cctacattga tacgtttatc accaatataa gtatggttaa ggctcaccaa ccatacattt
    18781 ggcaaatctc caagagtaga agcacctgtt ttgccatccc actgcgctaa cttccaatat
    18841 tcgttagctt tgtcaaggtc agaagatgtc attgctttgt caaggtactt agtcacggta
    18901 ggattgttat aaaacgtaat attggtccaa cctttacctg ctaggcttgg atgatgtgat
    18961 tcataaaatt gctgcgcgtg atgacgtcct ccggcataaa gtaaggctga gtcatgtgac
    19021 ttcgttgcca tttcatccca gttactagct ttgagtttaa tagtaattcc tagggctttg
    19081 gcttgctctg ctacttcaac ggctaagttc gctcgcaatt gatcattagt agggtagtac
    19141 agatcaaacg ctgcatcaag gtcacctttt ttacggctac cgtctgcttg ttctttccat
    19201 cccgcttttg tcaacaattg cttagcttta gctactttat tatctttaat ggctgttttt
    19261 ggattccaaa atggtgtttt atcaataatt gaataagctg gtttaccata accatttaaa
    19321 accgtatcga gaactttttg tctatttaaa ccaatagtca aggcttttcg gattgctgga
    19381 tcactagtga tatcatttcc tacaggataa ccatcaggag aatcagtgat gacgcctttt
    19441 ttcacataag gcaatgataa gccgcgcaca tcatttgatg gaatgtcaag gaggcgagtg
    19501 cctttgactt ttttatcagc aagttctggt gtcgcgtaga tcatatcaac atcaccagat
    19561 tctaaagctg ctagtgctgt gttttcatca agtaagaccc aagtccattt tttaaagtat
    19621 ggttttttcc catgccagta agggttacga acaaaaatag cttgttctcc agccttatat
    19681 tcttttacca tgtaaggtcc tgaaccgata ggattgctct tatacttatc attgtaatgt
    19741 tttttaggga cgattgggat ttcagtcaac tgtgctgtaa atgtcgaatg cgcctcagtc
    19801 aaatggatat tgacctgatt tttcccaact acttcaacgt tcttgatgaa ggttagatcc
    19861 caagcctttc catctgcttt caacatatca taagtaaact taacatcatc agcagtaaca
    19921 ggctcaccat ttgagaattt aaaatcatca tgcaagtcaa acgaccaagt cagcccatct
    19981 tcagagagat ggtatgtttt agcaagctct ccttttatat ctagttcagg agaacgtttc
    20041 aatagagtgc tatgagtgat attcccttca ttgtggactc cataacggtc ctttggatcg
    20101 aattcatgag ggagctttgc ccccatagaa acgacaattt cgtcttttgg acgttgtttg
    20161 cgctgacgtt cttttgtttg aggcttttgt tgttgacatg caactaaaat aagcccagtc
    20221 aaaaataacg tgataataga gaagtatttt aggtattttg acacaatcaa atctccttat
    20281 ttctaattaa taaataataa tagtaacgac tccatctcat tatagcaata aacaaatagg
    20341 agggcaattg tttaaccggt taattagaat ttttgttatt ttcaggaaga tactcagggc
    20401 ttttttataa gttgatgtat taacaaaaga taaagaggtt tcatgactta ttttagatta
    20461 aaaaaagaat taaactggtt actaagaagt aaaggaattg tagtttacga agggaatcaa
    20521 aaacttcaga tagctattaa tacaggtaaa aataacagtg aaaaagtctt ttttgatact
    20581 ttttttgagg tggttcgggc ggcagcgact ttctccacag caaaaaacat ctgttttggt
    20641 cttggcttac ttcgttcgca gaaataggaa atcgggtatc acattagcag agtagttcat
    20701 gctttttgag catcacactg ggtctgtcta cgttctaaaa gcttttgtc
//
LOCUS       DAUCWU010000021        20252 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUCWU010000021 DAUCWU010000000
VERSION     DAUCWU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20252
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.021"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            372..806
                     /locus_tag="VGY24_000752"
     CDS             372..806
                     /locus_tag="VGY24_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283754.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIAAE
                     TVEESLGQAKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(856..1767)
                     /locus_tag="VGY24_000753"
     CDS             complement(856..1767)
                     /locus_tag="VGY24_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ4283755.1"
                     /translation="MKTVRIFYNPNSGKKENQLARQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIMQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1884..2534)
                     /locus_tag="VGY24_000754"
     CDS             complement(1884..2534)
                     /locus_tag="VGY24_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ4283756.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2531..2971)
                     /locus_tag="VGY24_000755"
     CDS             complement(2531..2971)
                     /locus_tag="VGY24_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ4283757.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            3190..4038
                     /gene="ylqF"
                     /locus_tag="VGY24_000756"
     CDS             3190..4038
                     /gene="ylqF"
                     /locus_tag="VGY24_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ4283758.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            4028..4819
                     /locus_tag="VGY24_000757"
     CDS             4028..4819
                     /locus_tag="VGY24_000757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ4283759.1"
                     /translation="MSTSIKAIKESLEAVTSLLDPLFQELATDTRLGVQKALKSRQKA
                     IQTELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSIIKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4884..5720
                     /gene="dprA"
                     /locus_tag="VGY24_000758"
     CDS             4884..5720
                     /gene="dprA"
                     /locus_tag="VGY24_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ4283760.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEDRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5828..7957
                     /gene="topA"
                     /locus_tag="VGY24_000759"
     CDS             5828..7957
                     /gene="topA"
                     /locus_tag="VGY24_000759"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ4283761.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(8032..8916)
                     /locus_tag="VGY24_000760"
                     /pseudo
     CDS             complement(8032..8916)
                     /locus_tag="VGY24_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8929..9482
                     /locus_tag="VGY24_000761"
                     /pseudo
     CDS             8929..9482
                     /locus_tag="VGY24_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            9497..10489
                     /locus_tag="VGY24_000762"
     CDS             9497..10489
                     /locus_tag="VGY24_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ4283762.1"
                     /translation="MKLKLYNVRDEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLREMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDATKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10594..11259
                     /locus_tag="VGY24_000763"
     CDS             10594..11259
                     /locus_tag="VGY24_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ4283763.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     ADD"
     gene            11252..11968
                     /locus_tag="VGY24_000764"
     CDS             11252..11968
                     /locus_tag="VGY24_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ4283764.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHAFIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12119..13465
                     /gene="trmFO"
                     /locus_tag="VGY24_000765"
     CDS             12119..13465
                     /gene="trmFO"
                     /locus_tag="VGY24_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ4283765.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAESVTAELENHPLIEVIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NTFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13618..15024
                     /locus_tag="VGY24_000766"
     CDS             13618..15024
                     /locus_tag="VGY24_000766"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEQ4283766.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQVRQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEGLKRSQIGSAPVNTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            15067..15378
                     /locus_tag="VGY24_000767"
     CDS             15067..15378
                     /locus_tag="VGY24_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283767.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKLS
                     QQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVERK
                     S"
     gene            15378..15728
                     /locus_tag="VGY24_000768"
     CDS             15378..15728
                     /locus_tag="VGY24_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEQ4283768.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSQSAAPATEKEMNANA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            15738..16868
                     /locus_tag="VGY24_000769"
     CDS             15738..16868
                     /locus_tag="VGY24_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ4283769.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACCFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(17069..17953)
                     /gene="citG"
                     /locus_tag="VGY24_000770"
     CDS             complement(17069..17953)
                     /gene="citG"
                     /locus_tag="VGY24_000770"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEQ4283770.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(17946..18641)
                     /locus_tag="VGY24_000771"
     CDS             complement(17946..18641)
                     /locus_tag="VGY24_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4283771.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(18798..20201)
                     /locus_tag="VGY24_000772"
     CDS             complement(18798..20201)
                     /locus_tag="VGY24_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEQ4283772.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYISQ"
BASE COUNT         6407 a         3752 c         4103 g         5990 t
ORIGIN      
        1 gtcactttgt tgaaatgttt tcgtattaat gtttttttat ctattacgat agataatttt
       61 acttcaattt ttgattgaaa atactaaaat aagtgatgtg gtcactttga caaccattta
      121 ttggtccttt tagataagac aattttgtgg ctcttcagtt gaaaggatgt gttgttgatg
      181 ttagaggaaa caatcatgtt aaattaactc aggcttaaag accaaaaaag gagtgaagtc
      241 tattatgaaa aaaagaaatt ttaaacttac agaaatgtta caattggttt tattagtggt
      301 tcctgctgta aaacttgcta aaaaaatgtt taacgatagt aaaacaccgt ctaaaagaaa
      361 gtaggtctac aatgactaaa aaaaatcatg gcgttttttg ggggttgttg cttggatcag
      421 cagcagctag cattgcttac ttaagtttat cgtcttccaa aaaagatcaa ctcttgaaag
      481 atagtgctaa aaaaatagat gatctcaacg cttacttgca agataaaagt aagcaagttc
      541 tggatgcagt gtcggaaaaa gtacaagaat ccaaagatgc tgttgaagtt tatggtggta
      601 ttgccgcaga aactgttgaa gaaaagtggc aagataccaa agatgctgtt gaagtttacg
      661 gtggtattgc cgcagaaaca gttgaagaat ctttgggaca ggctaaagaa aaggtagaag
      721 gaattggcga agctactagc caaactatcc aatctaaaat ggaaaaactc accagcgata
      781 agacagatgc tgatgatgaa aaataggttt taaaatactt ttaagataag acaaaaagtg
      841 ccattaggca cttttttaat cttctgttgg aacaataatc gaaatggctt ttggaatgat
      901 atctaattga atgggcagat agtcactttt atccccatca atcctggtac gtggtaatat
      961 cgcttgtttt ttagcttgtg gtagtaagct aagcgtattg gcttgaaaat gactgatttc
     1021 ctctgctttg gaaaaatcac cctgtttaaa atcattaata tgccataaaa atttaaagaa
     1081 agagactttt ttcatggtat aaacttggaa atagccatcg tcagcttgag caccaggaga
     1141 aaaagaagga aaaccagcaa tggtatttgt catcgtgatt aagagaaact tcgttttcag
     1201 gtgaatacga tggttgtggc taattaaaga gatggcatag ctgcgatttc gtttcagtat
     1261 tcgaatacta tcttttaaaa atgctagagg accaaagcgt cgtttttcct cagccgttac
     1321 gtcggcagca atatcagcca gtaatcctaa ggttaaactg gaaatcatgt attgctggtt
     1381 agctttgcaa atatcaactt gcatgatttg tccagtcaag atagtatcca acgcctcagt
     1441 aatttgtaaa gggatatgca tagccttggc aaaattattg actgtccctg agggcactaa
     1501 gcctattaaa caatgagctc ctccttcata gatgccaccg atgattttat ttagtgtccc
     1561 atcgccacca agagggataa ccaagtcaat cttgtcctta gcagcttgtt ttgctaattg
     1621 aaaagcctgg tcagcatctt taggggtaat aacttctaca gaatcttcgg aaaaaccatg
     1681 ttggcaaaaa tagtctttga cttgcctagc aagttggttt tctttctttc cagaattagg
     1741 attgtaaaaa atccgaacgg ttttcatcag gtctctcctt tgacagctta ttacttgcta
     1801 tccattatac cacttctaag aattgatgtc ttacatcaaa tcatcacatt aagcctctca
     1861 atcagtctga gcatctattt gtcttaaagc ataaagaaag tgatagcgat aaattggagg
     1921 gcagaggcca ataaaataaa gagatgccaa atcatgtgga agtagggtct ttttttggca
     1981 taaaataccg ctccaatggt ataagacaag ccaccaaata acatgagaag accaaaagca
     2041 agactagttt tttgaatgat gactggcaag ataaacacaa cgagccagcc cataacgata
     2101 taaagcatca aactgaattt ttcattgata cgtttggcaa aaattttgta taaaatccca
     2161 aaaagcgtaa tgccccactg caaaacaata atgatatagc ctagccaacc agaaacaagg
     2221 gataaggcaa caggtgtata acttcctgcg atagcaatgt aaatcatact gtgatcaata
     2281 atgcgtagaa tgtatttgtg aaccgaacca taagccatgg agtggtaaat cgtagaggac
     2341 aaaaacatca aaaagagact gatgacaaag atggagatac caatagcggc tttaaggtca
     2401 taagtttgat aagcatagct ggctgaaata ggaagtagaa tgagcatagc aaatgctccg
     2461 atagcgtgtg tcacgctgtt tgctacttcc tcactaaaag ataagggtaa actttgttta
     2521 aaggtttggt tcattgggtc acctcctgtg ataaataaat agttttgtgg taatctttga
     2581 ccgtttgaca ggcaacctgc acaatcggtg gaatgtcctc ctcaatggga gctttctgat
     2641 tccaacaggt tacaaaagta ttgtagagac tttcagaatc agcctgtgcc tgaagttcct
     2701 ccaatacgcg actaatatct tgaataggaa ataccgtttt aaacaaactg atggcaatta
     2761 agcgcgctag ttgttgcttt tggtattttt tcttgatagg tttagtcaca tacccatgct
     2821 tgacgtaatt attaatcata gatgccgtta gtgatttatt atctgacact tctgaaaaat
     2881 cagtgcactg gttgacataa agtaagactt gatccaaata taagtctaaa tcagggaggt
     2941 ccttccaata aggtaatgat ggaaaaatca tagttttgtc tctctatcta gttttgataa
     3001 ctagattata cgattaatga aaacttattg caagcaaaaa ctgtaaagac ttaaaagagg
     3061 aaagtagaaa tggcgaagtg aagctaatca tgctataatg ttaaagaatt gattgggata
     3121 gatgccttct gtcgcaatca gactatttta tcacgaagaa gatgatgcta cgaaaggaat
     3181 gatacactca tggcaatgat tcaatggttt cctgggcaca tgtccaaagc cagaagacag
     3241 gttcaggaaa atgtaaagca tgttgacttt gtaactattt tagtggatgc acgcttacca
     3301 ctttctagtc aaaatcccat gcttacaaaa attgttggtg ataaacctaa attaatgatt
     3361 ttaaataaag ctgacttggc cgatgccact cgtacgaaag agtggaaggc ttattatgaa
     3421 agtcaaggta ttaaaacatt agccatcaat tccaaagaac aatcaacggt taaaaaagta
     3481 acagaagcag ccaaggaact catggctgat aaaattcaac gcttgcgaga gcgcggcatt
     3541 caaaaagaaa ccttgcgaac catgattatt ggcattccaa atgctggtaa atctaccttg
     3601 atgaatcgct tggctggtaa gaaaattgct gtagttggca ataaacctgg tgtgactaaa
     3661 ggacaacaat ggttgaagtc caataaggaa ttggaaattc tagacactcc tggtatttta
     3721 tggccaaaat ttgaggacga attggtgggc ttaaaattag ctttaacagg tgctatcaag
     3781 gaccaattac taccaatgga tgaagtgact atctttggct tgaattactt tagggaatac
     3841 taccctaatc gtttaaccaa acgttttaag aatattcctc ttgaggaaga agctcctgaa
     3901 atcattatga ccttgacacg ccaacttgga tttaaagacg attatgaccg tttttacact
     3961 ttattcgtta aggaagttcg tgatggtaaa ttaggtcaat acaccttaga tcaggtggga
     4021 gatatggatg tcgactagta ttaaagctat taaagaaagc ttagaggccg ttactagcct
     4081 cttggacccc ctctttcaag aattggcaac cgacactagg ttaggcgtcc aaaaagctct
     4141 aaaaagccga caaaaggcta ttcagaccga gttagcagaa gaagaacgat tagaagccat
     4201 gctttcttat gaaaaagctc tttataaaaa aggttatcaa gccattgcag gtattgatga
     4261 ggtgggacgt ggtcccttag caggtcccgt tgtggcagct tgtgtgattt tacctaagta
     4321 ttgtaaaatt aaaggcctta atgattctaa aaaaatccct aaagctaagc atgagaccat
     4381 ttatcaggca gtgaaagaaa aggctttggc tatcggtatc ggtattattg acaatcagct
     4441 gattgatgag gtcaatattt atgaagcaac caaactagct atgctagaag ccattaaaca
     4501 gttggagggc caactcacac aaccagatta tctcttgatt gatgccatga cattggatat
     4561 tgctatttcg cagcagtcta ttattaaagg cgatgccaat tccttgtcta ttgcagcagc
     4621 atcaattgta gctaaggtca ccagagatca gatgatggct aactatgatc gcatttttcc
     4681 tggttatggc tttgctaaaa atgcaggcta tggcaccaaa gaacatttac agggattaaa
     4741 agcttacggc ataacgccta tccatcgtaa aagttttgaa cctgttaaat ctatgtgctg
     4801 cgattcaact aatccttaaa aagttctgtt tttagcagaa tttttttctt ttttacgaat
     4861 aaatgaagta aggaggtaac ttagtgaatc attttgaact ttataagcta aagaaagctg
     4921 gactgactaa taaaaacatt ctcaatattc ttgactacca agaacaccag gaaaaatcgt
     4981 tgtcacttcg agatatggct gttgtttctg gttgtaagca tccgtctcac tttatagaag
     5041 cctataagca gttagatatt caaaaattaa aaatggaatt taaacaattt cctagtattt
     5101 ctatcttaga taagcattac ccaatggcat tgaaagaaat atacaatcca cctgtcctct
     5161 tgttttttca gggaaattta gaccttcttg agaaacctaa attagccatt gtcggctcca
     5221 gacgctcaag cgatactgga gtaaagtctg tccgtaaaat tcttaaagaa ctcgggaacc
     5281 gttttgtgat tgttagcgga cttgctcgag gtatcgacac tagtgctcat ttagcctgcc
     5341 ttaaaaatgg gggacaaact atcgctatta ttggaacagg attggatcgc ttttacccta
     5401 aagaaaatag ggagttgcaa actttcttag ggaaaaatca tcttgtgcta acagaatacg
     5461 gtccaggaga agaagcttta tcttatcact ttccagaacg gaatcgtatt atcgcaggcc
     5521 ttagccgagg tattcttgtc gttgaagcaa aaaatcgttc aggttccttg attacttgtc
     5581 aaattgggat agaagaagac cgagacattt ttgctgtccc aggaaacatt ttggacggga
     5641 aatccgaagg ttgccttcag ttaattaaag agggagcaac atgcgtcaca tcgggcatgg
     5701 atatcctttc agagtactaa aaaagttaaa gtttcttcct atatagcatt ttttttattg
     5761 acagtttctt aaaaatggga tattatgcta taagctttaa acaaactgtt ataggaagtg
     5821 tgtcgaattg ggaacaaaaa ctacaactaa gccaaaaacg ggtactaaaa agtccaccac
     5881 taaaaagaaa tcaacagcga aaaaaaatct cgttattgtt gaatcacctg ccaaagctaa
     5941 gactattgaa aaatatcttg gacgcagtta taaagttgtt gcttcggtag gacatattcg
     6001 tgacttaaaa aaatcgtcta tgtctattga ttttgataat aattatgagc cacaatacat
     6061 taatatccgt ggtaagggac cccttatcaa ttctttaaaa aaagaagcca aagctgctaa
     6121 aaaagtttat ttagctagtg acccggatcg tgagggagag gctatttctt ggcatttgtc
     6181 acatatctta ggacttgatc ctcaagataa taaccgagtg gtttttaatg aaattacgaa
     6241 agatgcagtt aaacatgcct ttgtggagcc tcgtcagatt gatatggact tggtagattc
     6301 tcaacaagca cgtcgtgttt tggaccgtat tgtgggatat tccatttcac caattctttg
     6361 gaaaaaggtc aaaaaaggac tttcagcagg acgtgtccag tcggttgctt taaaactcat
     6421 cattgaccgt gaaaatgaca tcaaagcatt tgttccaaag gaatactggt ctattgatgg
     6481 tctttttaaa aaaggaacta aaaagtttca agcgactttt tacggtatta atggcaaaaa
     6541 aacaaagctt gacaataaca acgatgtcaa agaggtatta gcaaagctaa caaatgaaga
     6601 ttttcttgtc agcaaggttg acaagaaaga gcgacgtcgt aatgctccct taccttacac
     6661 aacatcatct cttcaacaag atgctgctaa taaaatcaat ttcagaacgc gtaaaaccat
     6721 gatggtagcc caacaactgt atgaggggat tcatttagga gaaaatggaa ctcaaggttt
     6781 aattacctat atgcgtacgg attctactcg tatcagtcca gtagcacaaa acgatgctgc
     6841 tcaatttatt atcaatcgct ttggagctaa ttattcaaaa catggtaatc gtgtcaaaaa
     6901 taccagtggt gcccaagatg ctcacgaggc gattcgtcca tctagtgtta atcatacgcc
     6961 agactctatt gccaaatacc ttaacaagga tcagttaaaa ctttatacgc ttatttggaa
     7021 tcgttttgtg gctagccaaa tgacagcagc tgtttttgat accgtaaagg tgaacttgga
     7081 gcaaaatggc gtgatttttg ttgccaatgg tagccaaatg aagtttgatg gttacatggc
     7141 tgtttacaat gattccgata aaaacaaaat gttacctgag atggcagaag gagaaacggt
     7201 taaaaaaata tcgacctctc cagaacagca ctttacgcag ccaccagctc gttattctga
     7261 agcaactcta attaaaacgc tagaagaaaa tggtgttgga cgtccatcaa cttacgctcc
     7321 aaccttagaa gtcattcaac gcagatacta tgttaaattg tctgctaagc gctttgagcc
     7381 aactgagctt ggggaaattg tcaacaagct tattgtcgaa tttttcccag atattgtcga
     7441 tgttgccttt actgccgaga tggaaggtaa gctagatcaa gttgaaatcg gagaagaaca
     7501 atggcaacat gttatcgatc aattctatca accctttgtc aaagaattga ataaggctga
     7561 gtctgaaatt gaaaagattc aaatcaaaga tgaaccagct ggcttcgact gtgatgtttg
     7621 tggtcaccca atggtcatta agttaggacg ttttgggaaa ttttacgcgt gtagcaactt
     7681 cccagagtgc cgtaatacaa aagccatcac taaagaaatt ggtgttactt gtccggtttg
     7741 tcataaagga caagttatcg agcgtaaaac caaaaaaaat aggatatttt atggttgtga
     7801 tcagtatcca gattgtgagt ttatctcgtg ggatttacct attggacgtg cttgccctaa
     7861 gtcaggtgac tatcttattg agaaaaaagt tcgtggtggc aaacaagtaa tgtgtagcaa
     7921 tgagacctgt gattacaagg aagaaaaaat taaataaaaa aagaattgga actccttgtc
     7981 ttgttccaat tcttttttta tttacaaagg tatttttaac tacaaaaatc gctagttcgt
     8041 ttttaaaagg cttaaaaatt cctcaataac tggactgaaa ctcgcatcat ctcggtaagc
     8101 aatcccaata gggtaaaaat tatttttatc ttgcaaagga atccaaacta agttatctac
     8161 ctgattaaga ctcttaaaat cactcgggag gatagtcaag gtatttaaat cttgcaaact
     8221 atgaataaga acttcccaat cattgtgttt aaagacaata tctggttcaa aaccgagagc
     8281 tcggcatttt ctaggaagca tttcgcctag catgtagtga tcgtttaaag aagcaatctt
     8341 atagcctttt aaatctttaa gttttaactg aggatgttgc gccaaagggt gctgtttgag
     8401 gagaacgata ttgaccttat agtcttttgt ggacgtttgt aatagctcaa tggtaatatc
     8461 ctttcgaata cttagaaagg agaggagacc aatatcgatt tttcctcggg acaataattc
     8521 ttataatttg cgagaaccat cctgtataag cgataattca acatttgagt gagtagccat
     8581 aaagattgaa atttgtttca tgaattgcat agcaaacagt gatgttaatt caacacgaat
     8641 catctctttt tttcttgatt taattgctga atatccgcaa caatttgatt aaagtcatcc
     8701 acaaggtctt tgcctttgtg ataaagaata tctccagctt cggttattag tagctgtcaa
     8761 tcactttgtg taaaaagcgt cgtgttcaaa ctaggactct agccttttaa ttgattgaga
     8821 aagagtaggt tgcgttacaa acaaactttt agcagcgtgg gaatgatttt ttgtttcagc
     8881 aactgcaata aaataggtta attgtctaat atccatagta gatccttcat gattggtcaa
     8941 ggaatagcgt ctctacttgg tttacaacct attttgatgt ctctccttat tgccatgatt
     9001 ttctgcttcc ttattgtttc tccaattaca acggtaggaa ttgctcttgc gattaactta
     9061 tctggtattg gttcaggtgc cgcaagcttt ggactttgct tagcaggttg ggctgttaat
     9121 tcaaaaggaa cttcacttgc tcacgtcttg agatcaccga aaatttcaat ggccaacgtt
     9181 ttatcaaaac caaaaatcat gttaccaatg ttatgttctg cagctgtact tggtgttatt
     9241 ggtgctatct ttaatattca aggaacacct gctagtgcag gctttgggat tagtggtttg
     9301 atcggaccta tcaatgccct taatttagct aaaggcggat ggtctccagt aaatattcta
     9361 cttatcatta tctcattatc atttttgtag gggcacccat tgtgcttaat atgatcttta
     9421 actacctctt tattaaggtg cttaaagtga ttgatccaat ggattacaag cttaatattt
     9481 aagaaaggaa cttttgatga aattaaaatt atataatgtt agagacgaag aagcagtctt
     9541 agcaaaaaaa tgggctgatg acaatgggat tgagatttcc ttgacagagt ctcccttaac
     9601 ccctgaaacg gttaaagaag ctgaaggctt tgacgggatt gccaatgccc aaattggtcc
     9661 tttagatgat gcaatctatc ctctcttaag ggaaatgggg attaaacaaa tcgctcagcg
     9721 cagtgctggt gtcgatatgt ataaccttga tttggccacc gaaaatgaca ttattatcac
     9781 aaatgttcct agctattctc cagaatccat tgcagaattt acggttacta ttgcccttaa
     9841 tttaattcgt catgtggagt tgattcgtga aaatgttaaa aaacaaaatt tcacttgggg
     9901 actccctatc cgcggtcgtg ttttaggtga tatgacagtt gcgattatcg gaactggacg
     9961 tattggtcta gctactgcta aaatctttaa aggttttggc tgcaaggtcg ttggatatga
    10021 tatttaccaa agcgatgcta ccaaagctgt cttggactat aaggaatctg tagaagaagc
    10081 gattaaagat gctgatcttg tttccttaca catgccacca actgcagaga acacacacct
    10141 tttcaatgct gatttattta aatcattcaa aaaaggggct attttgatga atatggcgcg
    10201 tggtgctctc attgagacgc aagatttact ggatgctttg gatgcaggct tactgagcgg
    10261 agctggtatt gacacttacg aatttgaagg accttatata cctaagaatt ttgaaggtca
    10321 agaaattaca gattcactat ttaaagcttt gattaaccat cctaaggtta tttatacgcc
    10381 tcatgcagct tattatactg atgaagccgt taaaaaccta gttgaaggtg cccttaatgc
    10441 tacagtagag attatcaaaa ctgggacaac gacaactcgt gttaactaac aactgatttg
    10501 acgtccatta taaaggaata attttaaaaa ttcctttata atggactttt ttgcattagt
    10561 ccgttataat agactgtaag aaaggggact cttatgacat atatagcgct tattggagat
    10621 attatccagt ccaaacaatt gacggatcgt tccaaagttc aaaaaacttt agcagcttat
    10681 ttagacgacc tcaacaaaac ctttgctcct tatattattt ctaaattatc cctaacgttg
    10741 ggagatgaat ttcaaggact ttttcaggtg gatactccta ttttccatct gattgatttg
    10801 attaatcatc acatggatat ccctattcga tttggagtag gagtaggttc tattctaaca
    10861 gacatcaatc ctgatattag tattggggct gatggacctg cttattggca tgctcgtgag
    10921 gctattcgtt atattcatca aaaaaatgat tatggtaata ctacattagc tcttcggact
    10981 ggtcatcaca accaggatga tgtattaaac agtttactag cagctggaga tgctattaaa
    11041 gccaactggc gagctagtca atgggaaatt tttgacacgc ttttagattt gggcatttat
    11101 gaggaatact ttgatcaaca acgactagga aagcaattat ctcttagctc tagcgctttg
    11161 tcaaaacgtc taaaaagtag tcatgttaaa atttatctga gaacacggca gagtgcgcta
    11221 aactgtctca aacaaatcaa ggaggaagct gatgattaat ggtgtctctc actacctagc
    11281 tcaaacccct acacttaccc tgtttctcat ttgtcatgtg ttatcagact accagttaca
    11341 aagtcagcaa gtagctgatt taaaagaaaa acatttgact tatttaggtt accacttgat
    11401 tggagttagc attcccctaa tttgtttaac cctcattatc ccacaagcat ggttgatgag
    11461 cctattggtt atgataagtc acgcgtttat tgactggcta aagccaaaga tggctaactc
    11521 tctaaaatgg aaaagagagt ggatttttct gctggatcaa tgcttacata tcgctattag
    11581 ttcatttgct gggctaaggt tagcaggggt gaccttgcct aattggcttc cgatatctat
    11641 tttgatgacc gttttattta tcctactcat taccaaacca actaatattg tctttaaact
    11701 ctttttcagc aaataccagc ctgaccaagg agaaaaaatg gacacgatca taggagcggg
    11761 ggctaccata ggaattctag agagaattgt tattggggtg tgtatgatca tggggcaatt
    11821 tgcttcaata ggtctggtct ttaccgctaa atctattgct cgctataata aaatttcgga
    11881 aagtccagct tttgcagaat actacctaat tggttcttta tttagtattc tcagtgtttt
    11941 tatcgcagca tggatttgtt tcttctagtt gaaaagggtt cccgtagaaa gaggctaacg
    12001 cttagtctct ttctttttct ttcttcttat ttctgttata ataagtcagg taactacatt
    12061 agaaagattt gtgggtctgt catctaagag atgtcattcc cgaagaggta ttagtgactt
    12121 gtctcaatca actgcaactt atattaatgt tattggagct gggctagctg gttctgaagc
    12181 tgcctatcag attgctaagc gcggtatccc cgttaaattg tatgaaatgc gtggtgtcaa
    12241 agcaacaccg caacataaaa ccactaattt tgccgaattg gtctgttcca actcatttcg
    12301 tggtgatagc ttaacaaatg cagtcggtct tctcaaagaa gaaatgcgtc gattagattc
    12361 cattattatg cgtaatggtg aagctaaccg cgtacctgct ggaggagcaa tggctgttga
    12421 ccgtgagggg tatgcagaga gtgtcaccgc agagctggaa aaccatcccc ttatcgaggt
    12481 tatccgtggt gaaatcacag aaatccctga cgatgctatc acggttatcg cgacgggacc
    12541 gctgacttcg gatgccctgg cagaaaaaat tcacgcgcta aatggtggcg atggattcta
    12601 tttctacgac gcagcagcgc ctatcattga taaatctact attgatatga gcaaggttta
    12661 cctcaaatct cgctacgata aaggcgaagc tgcttacctc aactgcccta tgaccaagga
    12721 agaattcatg gctttccatg aagctctgac aaccgcagaa gaagcaccgc tgaatacctt
    12781 tgaaaaagaa aagtattttg aaggctgtat gccgattgaa gttatggcta aacgtggcat
    12841 taaaaccatg ctttatggac ctatgaaacc cgttggattg gaatatccag atgactatac
    12901 aggtcctcgc gatggagaat ttaaaacgcc atatgccgtc gtgcaattgc gtcaagataa
    12961 tgcagctgga agcctttata atatcgttgg tttccaaacg cacctcaaat ggggtgagca
    13021 aaaacgcgtt ttccaaatga ttccagggct tgaaaatgct gagtttgtcc gctacggcgt
    13081 catgcatcgc aattcctata tggattcacc aaatctttta acggaaacct tccaatctcg
    13141 gagcaatcca aaccttttct ttgcaggtca gatgactgga gttgaaggtt atgtcgaatc
    13201 agctgcttca ggtttagtag caggaatcaa tgctgctcgt ttgttcaaaa gagaagaagc
    13261 acttattttt cctcagacaa cagctattgg gagtttgcct cattatgtga ctcatgccga
    13321 cagtaagcat ttccaaccaa tgaacgtcaa ctttggcatc atcaaagagt tagaaggccc
    13381 acgcattcgt gacaaaaaag aacgttatga agctattgct agtcgtgctt tggcagattt
    13441 agacacctgc ttagcgtcgc tttaacacta aaaaagagtt ccttttcagt tgagttgctg
    13501 aagaggactt ttttacttgc tgatttgagc gctagctatg caaaagaatg cacaaaaaat
    13561 acatgaatag actatggtca ctagaagcaa tattatataa tgaatagtac tcattagttg
    13621 attcagtttg gaggttttag catgcagcag caagtcgcaa taacagaaac agttttacgg
    13681 gacggtcatc aaagtttgat ggcaacgcgg ttatctattg aggatatgct tcctgtcctt
    13741 accatcttag acaagattgg ttattattcc ctagagtgtt ggggaggagc aacctttgat
    13801 gcctgtattc gttttttgaa cgaagaccct tgggaaaggc tgcgcacgtt aaaaaaaggc
    13861 ttacctaata cccgtttaca aatgttatta agaggccaaa accttttagg ctaccgtcat
    13921 tatgcagatg acatcgttga caaattcatt agtttatcag ctcaaaatgg gattgatgtt
    13981 ttccgcattt ttgatgccct aaatgaccca cgaaatatcc agcaagcctt aagagctgtg
    14041 aaaaagacgg ggaaagaggc tcaactgtgt attgcctata caactagccc agtgcacacg
    14101 cttaactatt acctttcttt agtgaaagaa ttggttgaga tgggagctga ttctatctgc
    14161 atcaaagata tggctggtat tctaactccc aaagcggcta aagagttggt atctggtatc
    14221 aaagccatga ctaacttacc tcttattgtg catacccatg caactagtgg catttctcag
    14281 atgacctatc tagcagcagt agaagctggt gctgatagaa ttgataccgc tttgtcgcct
    14341 tttagcgaag gcacttcaca acctgctaca gaatccatgt atttagcctt aaaagaagca
    14401 ggttatgata tcacattaga tgaaacgcta ctggaacagg ctgctaatca ccttcgacaa
    14461 gtccgtcaaa agtacttagc agatggcatt ctggatccca gtcttctctt tccagaccca
    14521 aggacactac aataccaagt tcctggaggc atgttatcta atatgctatc tcagctgaaa
    14581 caagccaatg ccgaatcaaa attagaagaa gttttagcag aagttcctcg ggtaagaaaa
    14641 gacttgggtt atcctccctt agtaacaccg cttagccaaa tggtcgggac tcaagctgct
    14701 atgaatgtga ttttaggaaa accttaccag atggtgtcta aggaaattaa acaatacttg
    14761 gcaggcgact atggcaaaac accagcacca gtcaacgaag gcttaaaacg aagtcaaatt
    14821 ggctcagctc ctgtcaacac aaatcgtcct gcagatcaac taagtcctga attcgaggtg
    14881 ttaaaagcag aagtagcaga tttagcccaa acggacgaag atgtgctgac ttatgccctc
    14941 ttcccaagcg tagcaaaacc gtttttaaca accaaatacc aaactgatga cgtcattaaa
    15001 gtgactgcct ttatcaaagc ataataggct atcttaatca gatagaaaca aaaatatgga
    15061 gatcaaatga cctattcctt tattgatatc ttacaagtta ccttagtatc cttgttctta
    15121 gtgttaacct gtttaacagg gattatggga ttaatgatgc taagtggtaa attattgtca
    15181 caagaaaaaa aactctctca acaagcttcc agcattcctc aagagcaaga agctgacaag
    15241 ttgagtggag aagctctcga aactctttta gccaaagacg aattggctag aattgctgtt
    15301 atgactagtc ttgcttatgc tagccaagaa gagtcaggaa aacggtttga agttattgcc
    15361 gtggaaagga aatcctaatg aaaagatatg aagtgactgt caatggacag gtttatgaag
    15421 tgagtttacg tgaactagct gatggagaaa ctgttgaagt tagccagtca gcagcacctg
    15481 ctactgagaa agaaatgaat gccaacgctg ctggcggtgg cctacaggta aaggctccaa
    15541 tgtctggaac agtcttgtct atctttgcta cagaaggaaa agctgttaaa aaaggagaag
    15601 ctgttctggt cttagaggcc atgaaaatgg agaatgaaat tctagcacct gcagatggtc
    15661 tggtttcaaa aattcatgtt gtggctaacc aaacggtcga gtctgaacaa gttttgattt
    15721 cattctagga ggaaaccatg ctagacgtac tcaatcaaat ggttcaatcg tcaggattgg
    15781 ctcatctaac tgttaacaat cttatcatga tttgcttggc ctgttgcttt ttataccttg
    15841 gtatcaaaaa ggaatatgag ccttacctta tggtaccaat cgcctttggg attttactcg
    15901 ttaatttgcc tatggcggga ttgatggacc accctgccaa tggtaatcct ggtggccttc
    15961 tttattacct atataaggga actagcctag ggatttaccc acctttaatt tttctttgtt
    16021 tgggggcatc aactgacttt ggtcccttga ttgccaatcc taaaaccatt ttactaggag
    16081 gcgcagcaca agttgggatt ttcttagcct ttttcctcgc tattatgtta ggcatgacac
    16141 ctcaagaagc ggcttctgtc gggattattg gtggtgccga tggtccaaca gctatttatg
    16201 tgacgacaaa gttggcgcct gatttactct ctactatcgc cctagcagct tattcttaca
    16261 tggctctggt acctattatt caacctccca tcattaaact cttgactacc aaggcggaac
    16321 gccaagtgaa aatgactcaa gcaagaactg tttcacaaaa agaaaaaatc attttcccga
    16381 ttatggtaac aatctttgtc agccttttgg ttccaagtgc tacaaccttg gttggctgtt
    16441 tgatgctggg aaatctggtc agagaaatta aaattgttcc taaaattgta gagaatctcc
    16501 aacaagtggt tatgttttgc attactatca ttttagggtt aaccgttggt gctaaggcca
    16561 atggagatct ctttttatca gccacaacct tgaaaattat tgcccttggg ctaatcgcct
    16621 ttgctgctgg tacagcaggg ggtgtcttga tggggaaggt catgtactat ttatctggta
    16681 acaaagtgaa ccctatgata ggagcagcag gtgtttcagc tgttccgatg gcagcgcgag
    16741 tggtccaaaa ggtcggacaa gaagaagatc caagtaattt cttactcatg cacgccatgg
    16801 ggcccaatgt agctggcgtt attggctcag ccattgcctc aggtgccttg ctggccttct
    16861 ttggctaatt gtgaaaaagg attagtaaat taatcttaat aaatcattat caggagttgg
    16921 aagcaatcta gctccttttt tagtagaaag ttcgagtgat aaactattcc aaaataatta
    16981 taaaaaaaga ctgtcctgat tgtacggact atatcaattg acttttatag tctagctgaa
    17041 atagcagtgc aaaactaaga cactcgtttt aaagtagttt ttctaaaaaa gcaaaataaa
    17101 aggtcagagc caataaatct gccgcgcccc cagggcttaa atgctgatta atgaggcatt
    17161 gattgtagga ggacaaagcc aatcttaggt cagttgttga taaatcttgt tgcaggagta
    17221 gtcgcatatc tgccttaacg cctttccatg cttcaatccc tccacgatga atcagatttc
    17281 catcttcaac aatagacatc aaataaacaa gaagacgaag ctgactggtt tcggggtcat
    17341 tttgacttat catctgacga aagtagggca aggcatggtc tgttaaggtc gtgaatccct
    17401 cgcttgcctc accccgaggt cctttgatgc cataagttac aaagagttgc tccccataag
    17461 tgaactcttt tttagtgttc aaatgaccaa ggtctgtttg aatcaaatgt gccgtcatag
    17521 gttttactaa acgacagata gctagagtat cttccttgct aaaacaccct aaatcagtca
    17581 ttaagtgagg tgttcttgct agataggccc cagttgcacc aagaagtaaa gccatggaaa
    17641 aattcaggcc tttatgagtg ttaattccct gagtagcaca aaacatggtt tcctcggcct
    17701 tttgacctag ttgtctaaga cggttaaaca gaagaagtgg ctcttctttt gcataagcaa
    17761 aacctgtttc gatataagcc tgaaaaaaag gagagagtgc tatcatactg tcgataaagg
    17821 tcataaagga catgtcatca tgggcaccgt tatcaaatcg atcaactaaa ccaggttttg
    17881 gagatagtga gacttcataa agcaaagctt ttaaggccag ttgactaata cttgttagaa
    17941 ctgccttagt cattttgctc aggcctcatt tcttttaaga taaattcaag tgagctattc
    18001 aagtgttcat gtgtaatcaa cgtaatttgc tcatgatctt tgttcttcat acctcggtaa
    18061 atcatccagt gttcagatag agctcgttta cggcgttcaa ctgaagaaat cgaaattttt
    18121 ctaaagtaga ccaaataagc ttgcaattct gtcacaattt cttttaagcg gaccatttgg
    18181 ctatagctgt aaatcaaatt gttaaaggca ttaaaattat ccaaaatacg attggtgtca
    18241 tcctctgcga taaaggtttc acagtcttcc aacaagttat gcatgacttt aaaatcttct
    18301 tctgtcatta aatgcatcgc ttgcactgtt gcgagagtct ctagagcttt tcgaatttca
    18361 aaaatttcac aggcatcctt gatactaact cctttgacaa taatgccttt tttaggaatg
    18421 tgctctacaa ggtgttcttc agacaataat cccaaagcgt agcgaatagg tgtcctgcta
    18481 atattaagcg caatggaaaa ctctttttca ttaatgcggc ttccaacagg aatttgtcgc
    18541 aaaataatgg tctttttaag agcgttataa aaggctattt tcaaagggat atttcttgat
    18601 aggtcaagat tttgtttaac ggcttcgata ataggattca tgtcatctcc ttaatgctag
    18661 tgtcctcact ttattatact taaatttgtg aaacattcca ataggactag atgagttctg
    18721 aaaaattaaa gccatgctca caaaaggaca tgactttaat catctcataa gaataaggta
    18781 gtagtgttat cgttggttta ttgtgaaatg taaagtggca tttgtcccat ggcaataatg
    18841 gttaccacaa agataacaaa gatgataagg gcatatttag cagcctcttt ttgccattcc
    18901 cccatgtcaa gacctgtcaa gcgaagaaga agatagatga aggcaactaa tggactaagt
    18961 aagtggaagg cttgtcccat gagggaagca agtgccatag ccatgttact gaaaccatat
    19021 tcagcaccag cttctgcaag aacaggcaag ataccatagt aaaaaccatc attggagata
    19081 aagaaggtac caggtgcaga aactaaggca ataacgagac cccagaaacc tgccagttgt
    19141 tttggaataa tggttgcaaa actttgtgca agagcgctag ccataccaga accttgaaaa
    19201 agacccataa agataccagc agcaaagacg agaataacca cttgaaccgc atcaccagca
    19261 ttgtcaccaa tgcgttttga ttgatcttta agcactgggt aattgaccat caaggcgata
    19321 caagttccca ccgcaaaaag aagaagcggt gccatagcaa tagatttgtt gaaagagcca
    19381 gcaactaacc aagtgattaa tccaatggtt aagatagcat tgaaaacaaa gtttttagga
    19441 cgacgaataa aaagggtatc tgggtcagta atgtctgtga gttgacgcat gtcttcttca
    19501 gacaagtcaa tcacaccaag tcttgcacgt tctttacgcc ccatgcttgg agcaacccaa
    19561 caaatcacat aaagaagaga taaaatcata cccggtgcaa gatagccaag aatttcagga
    19621 ccgacaccaa gaacagacat cgcacgagca gtagggccac cccatggcag taagttcata
    19681 atagtatttt gaagaataat caagacacct aggttcatga ttttcatgtc caatttttta
    19741 tagataggta agaaagcaga gcaacaaatt aaagtagtgg ttgttccatc accattaaga
    19801 gatactgcag cagcaacgac agctgttgcg ataagcacct tcattggatc acctttagca
    19861 aagcgaatca tcttttcagt aatcggatca aacaatcctg catctaacat gacagagaag
    19921 aacaaaatgg caaatagcaa catgatacct gttttagcag tattgttgat accatacata
    19981 accattggtc cgatagctgt taggtcttta gcaattccac cgtcaccaac aaaggcaaca
    20041 aatttggcat ctgcattgaa gtcagctgaa ccagtcagta taacagcaat cgtcatgatt
    20101 aatggaatca tgaccaaagc agtaaaaggg gtcatttttt tcttcattac cacgtacata
    20161 aagacaataa tcatggcata agccagcatt gttaataaca tcattgttat ctcctttttc
    20221 aagttatgat ttcagtatag atgaaagcgc tt
//
LOCUS       DAUCWU010000022        20126 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUCWU010000022 DAUCWU010000000
VERSION     DAUCWU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20126
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.022"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            53..637
                     /gene="folE"
                     /locus_tag="VGY24_000773"
     CDS             53..637
                     /gene="folE"
                     /locus_tag="VGY24_000773"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ4283773.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            646..1446
                     /gene="folP"
                     /locus_tag="VGY24_000774"
     CDS             646..1446
                     /gene="folP"
                     /locus_tag="VGY24_000774"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ4283774.1"
                     /translation="MKIGRFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCRFVSATNEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDVPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            1453..1812
                     /gene="folB"
                     /locus_tag="VGY24_000775"
     CDS             1453..1812
                     /gene="folB"
                     /locus_tag="VGY24_000775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ4283775.1"
                     /translation="MDKIVLEGCRFYGYHGAYKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            1809..2309
                     /gene="folK"
                     /locus_tag="VGY24_000776"
     CDS             1809..2309
                     /gene="folK"
                     /locus_tag="VGY24_000776"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ4283776.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            2459..3346
                     /gene="murB"
                     /locus_tag="VGY24_000777"
     CDS             2459..3346
                     /gene="murB"
                     /locus_tag="VGY24_000777"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ4283777.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGILGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            3392..4546
                     /locus_tag="VGY24_000778"
     CDS             3392..4546
                     /locus_tag="VGY24_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283778.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKKFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEEGLINAIEEERNEENF"
     gene            4530..5324
                     /locus_tag="VGY24_000779"
     CDS             4530..5324
                     /locus_tag="VGY24_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283779.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGHVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     SRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            5321..6097
                     /locus_tag="VGY24_000780"
     CDS             5321..6097
                     /locus_tag="VGY24_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4283780.1"
                     /translation="MKKFANLYLASIFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            6090..7163
                     /locus_tag="VGY24_000781"
     CDS             6090..7163
                     /locus_tag="VGY24_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4283781.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFIFQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPIHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(7218..7883)
                     /locus_tag="VGY24_000782"
     CDS             complement(7218..7883)
                     /locus_tag="VGY24_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ4283782.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(7864..9405)
                     /locus_tag="VGY24_000783"
     CDS             complement(7864..9405)
                     /locus_tag="VGY24_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4283783.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKALLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLEKPIGKPFKFLFPSFPNLQIQEGHEQ
                     LFRYDEEDYLLAISPIAVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSFVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            9566..10897
                     /locus_tag="VGY24_000784"
     CDS             9566..10897
                     /locus_tag="VGY24_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ4283784.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVNVGFFVSRFLNMNSVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            10928..12094
                     /locus_tag="VGY24_000785"
     CDS             10928..12094
                     /locus_tag="VGY24_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ4283785.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVAS
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLKKSLDEVSIVVNGGGSAGLFITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(12176..13225)
                     /locus_tag="VGY24_000786"
     CDS             complement(12176..13225)
                     /locus_tag="VGY24_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEQ4283786.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVKPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKTFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVIIDNDIS"
     gene            13461..14192
                     /gene="aphA"
                     /locus_tag="VGY24_000787"
     CDS             13461..14192
                     /gene="aphA"
                     /locus_tag="VGY24_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ4283787.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMCKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            14370..15902
                     /locus_tag="VGY24_000788"
     CDS             14370..15902
                     /locus_tag="VGY24_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ4283788.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            16065..16679
                     /locus_tag="VGY24_000789"
     CDS             16065..16679
                     /locus_tag="VGY24_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ4283789.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQSYI"
     gene            16838..17986
                     /locus_tag="VGY24_000790"
     CDS             16838..17986
                     /locus_tag="VGY24_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ4283790.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            18035..18715
                     /gene="radC"
                     /locus_tag="VGY24_000791"
     CDS             18035..18715
                     /gene="radC"
                     /locus_tag="VGY24_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ4283791.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(18717..19412)
                     /locus_tag="VGY24_000792"
     CDS             complement(18717..19412)
                     /locus_tag="VGY24_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ4283792.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(19422..20066)
                     /locus_tag="VGY24_000793"
     CDS             complement(19422..20066)
                     /locus_tag="VGY24_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ4283793.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT         6047 a         3455 c         4167 g         6457 t
ORIGIN      
        1 gttcttttaa cccattaaaa aagagaaaat gaaaaggaaa aggaaaaaac taatgaaaag
       61 ggaaagacta atgtctatta ataaggaaaa agcagaagca gccatttatc agtttttaga
      121 ggctattggt gaaaatccaa atcgggaagg tcttcttgac acgcctaaac gggttgctaa
      181 aatgtatgcg gagatgtttt tgggactggg aaaagatcct aaagaggagt ttacggctgt
      241 ctttaaagaa caacatgagg atgtggtgat tgtcaaagac attagttttt attctatatg
      301 tgaacaccac ttagttcctt tttatggcaa ggctcatatc gcttacttgc caagtgatgg
      361 tcgagtaaca ggtttgagta aattagcgcg agcagttgaa gtagctagta aacgacctca
      421 actccaagag cgtttgacct ctcaaatagc ggatgcttta gttgaagccc tcaatcctaa
      481 agggacttta gttatggtag aagcagagca catgtgcatg accatgcgag gcatcaaaaa
      541 gccaggtagt aaaaccatta ccaccactgc cagaggctta tacaaggaaa gtcgtgctga
      601 gaggcaagaa gttatttctc taatgacaaa agattaggag gacttatgaa aattggaagg
      661 tttgtgattg agggcaatgc ggctatcatg gggattttaa atgtgactcc agattctttt
      721 tcagatgggg ggtcttacac tactgtgcaa aaagcattag atcacgttga gcaaatgatt
      781 gctgatggtg ctaaaatcat cgacgttggt ggagaatcaa cacgtccagg ttgccgattt
      841 gtaagcgcta ccaatgaaat tgaccgggtg gttcctgtga tcaaggccat caaagaaaac
      901 tatgatattc taatcagcat tgatacctat aaaaccgaaa cagctagagc agctttagag
      961 gcgggtgccg atattctcaa tgatgtttgg gcaggtttgt acgacggaca aatgtttgcc
     1021 ttagcagccg agtacgatgt gcctatcatc ttgatgcata accaagacga agaagtttat
     1081 caagaggtaa cacaagacgt ttgtgatttt ctaggcaata gagcacaagc agctcttgat
     1141 gctggcgtgc caaaaaacaa tatttggatt gatccaggat ttggatttgc caaatctgtt
     1201 caacagaata tggagttatt aaaaggattg gaccgcgtct gtcagttggg ctatcctgtc
     1261 ttgtttggta tttcgagaaa gcgtgtcgta gatgccttgt taggcggcaa caccaaagct
     1321 aaagagcgag acggagcgac agcagccttg tctgcttatg cccttggaaa aggctgtcag
     1381 attgtacgcg tacacgatgt caaggctaat caagacattg tggctgtgtt gagccagttg
     1441 atgtgaggac ttatggataa aatcgtatta gaaggttgcc gtttttatgg ctaccatgga
     1501 gcctataaag aagaacagac ccttgggcaa atttttctgg ttgatttaga attgtcagtc
     1561 gatttgcaag cagcttcttt gtcagaccaa ttaacagata cggtccatta cgggatggtg
     1621 tttgatagtg tccgtcagtt agtggaaggg ggaaagttta ttttgattga acgtttagca
     1681 ggtgcgattt gtgaacagct ttttaacgaa tttccaccta ttgaagccat caaagtggcc
     1741 attaaaaagg aaaacccacc gattgcaggc cactataagg cagttggtat tgaattggag
     1801 agacagagat gaccattgtt tatttaagtt taggcaccaa tatgggggac cgagcagctt
     1861 acttgcaaaa agctcttgag gctttagctg acttacctca aacacggttg cttgctcaat
     1921 catccattta tgaaacgact gcttggggca agacaggcca ggctgatttt ctcaatatgg
     1981 cctgtcaatt agatacacaa ttaaccgcgg ctgatttctt aaaagaaaca caagctattg
     2041 agcaatctct tggtcgtgtg aggcatgaaa aatggggatc aagaactatt gatattgaca
     2101 tcctgctatt tggagaagag gtttatgaca caaaggaatt gaaagtgcca cacccttata
     2161 tgactgagcg tgcttttgtt ttaatcccct tattggaatt gcagccagat ttaaaattgc
     2221 ctcctaatca taagttttta agggattatc ttgcagcttt agatcaatca gatatcacgc
     2281 tcttttcagc tcaacagaca gagttctaga ctctgttttc ggagtaatgc ctgctataag
     2341 ccaatccata aagttttgcc aaattcatcg ttattttttc taaaatttga tataatagta
     2401 tcggctttat gccgattttt ttacgcgtat aagaagtgat aaaagaaaga agataactat
     2461 gataactgaa cttcatggga ttgatattcg agagaatgaa cccctaaaac attacactta
     2521 cactaaggta ggtggcccag cagatttctt ggcttttcct agaaatcact acgagctatc
     2581 acgcatcgtt gcttatgcca acaaagaaaa tatgccttgg cttgttttgg gaaatgccag
     2641 caatctcatt gtgcgagatg gtggtattct tggctttgtc atcatgtttg ataagctaaa
     2701 tgcagtgcac ttaaatggtt ataccttaga agccgaagct ggtgccaatc tgattgaaac
     2761 aacgaagatt gccaaattcc atagtttaac agggtttgaa tttgcatgtg gcattcctgg
     2821 aagcattggg ggtgctgttt ttatgaatgc aggtgcctat ggtggggaaa tatcacatat
     2881 tttcttatcc gctaaggtgc ttacctcaag tggagagatc aaaaccattt cagctaggga
     2941 tatggctttt ggttaccgtc actctgccat tcaagaaaca ggtgacattg tcatttctgc
     3001 taagtttgct cttaaaccgg gtaattatga tacgatcagc caagaaatga atcggttaaa
     3061 tcaccttcgc caactaaaac aacctttaga atttccatct tgtggatctg tgtttaagcg
     3121 cccgccagga cactttgcag gtcaattaat catggaagca aatcttaaag ggcatcggat
     3181 tggtggtgtg gaagtttccg aaaaacacgc tggttttatg attaatgtgg cagatggcac
     3241 agctaaagat tatgaagatt tgattgctta tgtcattgag acagttgaaa accattctgg
     3301 tgtcaggctt gaaccagaag ttcggattat tggggaaaac ctgtaaccat tattgataat
     3361 taaagaaagt accgtggagg atttatgaca attgactaag ccaattatca catttaacaa
     3421 tgtttcaaaa acatttgagg acagtggaac acaggttcta aagaacatta actttgacct
     3481 tgaagaagga aaattttaca ccctgcttgg agcttctggc tctggaaaat caactatttt
     3541 aaatattatg gcgggcctac tagatgccag cagcggagac atttatttag atggggaacg
     3601 tattaatgat ttgccgatta ataaacgtga cattcatacc gttttccaaa attatgccct
     3661 cttccctcat atgactgttt ttgagaatgt tgcctttgct ttgaagctga agaaagtgga
     3721 caagaaagag attgctaagc gcgtgaaaga aaccttgaaa atggttcaat tagaagggta
     3781 tgaaaatcgt tctatccaga agttatctgg tggtcaacgt caacgtgttg ccattgcgcg
     3841 tgcgattatc aatcaaccgc gtgtggtctt acttgacgaa ccactctcag cccttgattt
     3901 gaaattaaga acagagatgc aatatgaatt acgtgaattg caacaacgtc taggtattac
     3961 ctttgttttt gttactcacg atcaagaaga agccttggcc atgagtgatt ggatttttgt
     4021 catgaatgaa ggtgaaattg ttcagtcggg aacaccagtg gatatttacg atgagccaat
     4081 taatcatttt gttgctaatt ttattgggga atctaatatt attaacggta ccatgattga
     4141 agactatctt gtctccttta acgggaaaaa atttgaatct gtggacggtg ggatgcgccc
     4201 taatgagcct gttgaagtgg ttattcgtcc tgaagatctt caaattactt tgccagaaga
     4261 agggaaatta caagttaagg ttgataccca attattccgg ggagttcact acgaaattat
     4321 tgcctatgat gaattgggta atgaatggat gattcattct acccgcaaag ctatcgaggg
     4381 agaagttatc ggattagatt ttacccctga agatcttcat atcatgcgtc ttaatgagac
     4441 tgaagaggaa tttgatgccc gtattgaaga atatgtggaa atggatgagc ctgaagaggg
     4501 attgattaat gccattgagg aggagcgtaa tgaagaaaac ttctagtctt ttttcgattc
     4561 cttacttctt atggattctc ttctttgttg tggcaccagt cactctcttg ttttacaagt
     4621 ccttttttga cattgaaggg catgtgactt tagccaatta cgaaacgttt tttagctctt
     4681 ggacttattt gagaatgagt gtgaattcta ttttatacgc tggtattatc acactcgtca
     4741 cgctcttgat ttcatatcct acggctctct ttttaacgcg cctaaagcac aagcagttgt
     4801 ggcttatgct cattattttg ccaacttggg taaacttatt gctaaaagcc tatgccttta
     4861 tgggaatctt tggtcaacaa ggaggaatta acagcttttt aacctttatg gggattggcc
     4921 cgcagcaaat cctgtttacg gatttctcct tcatttttgt agcctcttac attgagctcc
     4981 cttttatgat gttaccgatt tttaacgctt tggatgatat tgaccataat gtcatcaatg
     5041 ccagtcgcga cctaggagct agtgaatttc aggccttctc aaaagttatt tttccccttt
     5101 ctttaaatgg ggttagggca ggtgttcagt ctgtctttat cccaagtttg agtctcttta
     5161 tgttaacccg tttgattggt ggaagccgcg tgattacact tggtacagcc attgaacaac
     5221 attttttgac cacccaaaac tggggaatgg gatcaaccat tggtgtcgtc ttgattttga
     5281 ccatggttgc tattatgtgg ctcacaaagg agaaaagtaa atgaaaaaat ttgccaatct
     5341 ttatttagcg agtatctttg ttttactcta cattcctatt ttttatttga ttttctattc
     5401 tttcaacaaa ggtggggata tgaatggttt tacaggattt acccttgagc attaccaaac
     5461 catgtttgag gatagtcgtc tcatgacaat cttactgcaa acctttgttc ttgcttttag
     5521 tagcgctcta ctagcaacga ttattgggac ctttggagct atctttatcc accatgttag
     5581 aggtaagtac caaaatgcca tgctatcagc caataatgtc ttgatggtat caccagatgt
     5641 catgattggg gcttcctttt taattctttt tacatcattg aagtttcagc tgggcatgtc
     5701 ttcagtttta ttaagtcata ttgctttttc gattcctatt gtggttttga tggtattgcc
     5761 gcgcttgaaa gagatgaatc aggacatggt caacgccgct tatgatttgg gagctaatta
     5821 tttccaaatg ctcaaagaag tcatgctgcc atacttaaca ccagggatta ttgcaggtta
     5881 ttttatggcc tttacctatt ccttagatga ttttgcagtg actttctttt tgactggaaa
     5941 tggttttact actttatctg ttgagattta ttcgcgggct cgtcagggaa tttccttgga
     6001 tatcaatgct ttgtcaacca tcgttttctt tttctccatc ctcttagtga tcggttatta
     6061 ttatatgtca caggacaagg aggaaaaaca tgcgtaaact ttattccttt ctagcaggag
     6121 ttttgggtgt tattgttatt ttaacaagtc tttctttcat ctttcagaaa aaatcgggtt
     6181 ctggtagtca atcggataaa ttagttattt ataactgggg agattacatt gatccagctt
     6241 tgctcaaaaa attcaccaaa gaaacgggca ttgaagtgca gtatgaaact ttcgattcca
     6301 atgaagccat gtacactaaa atcaagcagg gcggaaccac ttatgacatt gctgttccta
     6361 gtgattacac cattgataaa atgatcaaag aaaacctact caataagctt gataagtcaa
     6421 aattagttgg catggataat atcgggaaag aatttttagg gaaaagcttt gacccacaaa
     6481 acgactattc tttgccttat ttctggggaa ccgttgggat tgtttataat gatcaattag
     6541 ttgataaggc gcctatacac tgggaagatc tgtggcgtcc agaatataaa aatagtatta
     6601 tgctgattga tggagcgcgt gaaatgctag gggttggttt aacaactttt ggttatagtg
     6661 tgaattctaa aaatctagag cagttgcagg cagccgagag aaaactgcag cagttgacgc
     6721 cgaatgttaa agccattgta gcagatgaga tgaaaggcta catgattcaa ggtgacgctg
     6781 ctattggaat taccttttct ggtgaagcca gtgagatgtt agatagtaac gaacaccttc
     6841 actacatcgt gccttcagaa gggtctaacc tttggtttga taatttggta ctaccaaaaa
     6901 ccatgaaaca cgaaaaagaa gcttatgctt ttttgaactt tatcaatcgt cctgaaaatg
     6961 ctgcgcaaaa tgctgcatat attggttatg cgacaccaaa taaaaaagcc aaggccttac
     7021 ttccagatga gataaaaaat gatcctgctt tttatccaac agatgacatt atcaaaaaat
     7081 tggaagttta tgacaattta gggtcaagat ggttggggat ttataatgat ttatacctcc
     7141 aatttaaaat gtatcgcaaa taagcactca aaaaggctgg tatttctcca gcctttttaa
     7201 tgtatcgaca gatgacgtca ccagtacacg cgataggggc ggccaacctt tgtaaaaatt
     7261 tgttggctgt tcagttgctt attttcttct aaataagcaa tgtatttacg gacagagaca
     7321 tgggataagt gacaagcgga agctaattct tggatagtaa aaggttgatc aaaggcttta
     7381 atattttcca taatccactg gaaagtggat tcagataagc ctttttctaa caattgtttg
     7441 tttttagtgt cttttttgct agtcaaacat ttcagttggt caatttgggc ctgctctagt
     7501 tgctgattgg ccaaatgttc tcgatgtgta acaaactgtt ggatgctttc ttgaaagcgt
     7561 tcaaaggtaa agggcttgat gaggtagtcg ataattccta aatgaaaacc atctcggata
     7621 atattcccat catttgcggc tgaaataata atgacttcgc aaggaatatg ctgagcacgc
     7681 catttctcca aaaattgaat cccattgcca tctgtaatat gaatatccag taaaatgagg
     7741 tcaattgcgt aatcagtaag tatactttga acagctttca tagagtcgct ggatataatg
     7801 cgatcaaaaa gatttagttt ttcgaggtaa ttgcgatgga taaaatccac cataggatca
     7861 tcttcaatga ttaaaacgtt catgttgccc tcctacataa ggtgttgtca ctgagaactg
     7921 taactcggtt acggtttgat gttgatggaa ttgggaattt gtatcggtca agagctgttg
     7981 aaaataaggt aagtcaaaaa taggctgtac ccgctccaaa tcaatccaat tgtctgtcaa
     8041 ggtgtaatgg ctgattaaat gattctgatc atgttgaata ctcaaaacga atgatttggg
     8101 tctcagtgtg gttagaagat tagtatggat aaagcggtaa atcatcaaac cattattaag
     8161 ttgattcttg gtggcagaat gaggaatctc gcttaaaaca tcaatcttga gatggacatt
     8221 taattcttgg tatttctctt gctctccaat gagaaaacta gctagaagag gctccttgac
     8281 aaggacagat aagctagtga gaatttcatt ttcgggctcc aaaatactgt ctaaataaat
     8341 tttcaattgg tcataataag caatatccac taagccataa ataacatgca attgattcat
     8401 gaatttatga gtttgggctt gtagagcaga agcataggca gtggtatagg ctagctgatc
     8461 caaggtatca atagccttaa ctgcttctcg catgaaaata atatggcctc ggtgatcgtt
     8521 tttgacagca attggagaga tagcgaggag gtaatcttct tcgtcatacc gaaacagttg
     8581 ttcgtgccct tcctggattt gaagatttgg aaaggaagga aaaagaaatt taaaaggttt
     8641 tccaattggc ttttctaact ggcatttccg agcaattaat ttttgtccag cctgattgca
     8701 gagctgtaag ataccagctt tatctaccac aaatacggcg gcttcgattt gatccaacat
     8761 ggcattacgc tcttcaaaga gctggtaaat ttcacttggt tctaactggt gcagttgacg
     8821 cttgagtcga aaagaaataa aactggtgac cagtaaacta ataagaagac aaaggagtaa
     8881 agacagggta taattgcgtt ttgacgttaa agccacatca tttagggtag ttagtttaat
     8941 gccaaccgca atagcaccaa tctgtttgtc accgtcaaac acaggtacta gatagcgaag
     9001 tgactttcct aaagtgccct tggcggtaga gataaccttt tttcctgcta gaacagcctc
     9061 ctcatcacct ccttgaaaag gttttccgat atttttcggg ttaggatggg tgagtcgaat
     9121 ccctttcatg ttcatcacga ccacataatc aaggttatag atggaggcaa tggagttggt
     9181 ataggctgtt gttttagcat ttggttggtt gtttaggagt aatgctttaa tcgcttgatg
     9241 acttgctaac atttttccag ttgacgttag caaatgggtt tcctgatttt tgatggattg
     9301 gtgcgtatca tgtagcataa tcccgtaaaa tagagaggtt gtgacaacaa tcatagatac
     9361 caaaatcaat gataaactgg cccataaacg tagtggtttt ttcataaggt ctcctcttta
     9421 ttgagtataa cacgataaga aatgacctgc tagcttaaat taattaaata acttgaaaaa
     9481 attaaagtag ttaaacaact taaataaact atgcttttga catgttctct tttatactag
     9541 ccttatcact aaaggaggtt gtttcatgga aacaataagc aagaaaatgc ctcaaaaaga
     9601 cttgtctgag cattcaaaag catggcaaaa taggcgaata ggctccgttc ccttgcctgt
     9661 ttatctggtt ttagctacct taattttagt cacaggctgg ctccaacaat tgcctgttaa
     9721 tatgctgggg ggctttgctg ttattttaac ccttggttgg ctactaggaa ccattggagc
     9781 gaccattcca ggcctcaaac attttggtgg cccagctatt ttatcactct tagtcccttc
     9841 gattttggtt ttttttaatc tcctcaaccc caatgtctta gaagcgacga atgtcttgat
     9901 gaagcaagcg aattttcttt atttttatat tgcctgcctg gtctgcggta gtattttagg
     9961 catgaatcga aaaatcttga ttcaaggtct ttttcggatg attattccca tgttacttgg
    10021 aatggtttgt gcaatgggtg tagggacttt ggttggcgtc attttaggat tagactggca
    10081 acataccctc ttttatgttg tgacgccagt ccttgctgga ggaattggtg aaggtatttt
    10141 acccttatct cttggttaca gtgccattac aggagtcgga agtgagcaat tagtggctca
    10201 actgattcca gccaccatta ttgggaattt ctttgctatc ttgtgcacgg ctttgctcaa
    10261 tcgttttgga gagaagcacc caagttattc tggtcaaggg caactggtta aaattggtca
    10321 cagcgaagac atgtctgatg ccctaaaaga caattctgga gccctagatg ttaaattaat
    10381 gggggcaggc gttttaacag cttgttccct ttttattgct ggtggcttgt tacagcattt
    10441 aacaggtttt ccaggaccgg tgttgatgat tattctggca gccttcttga aatacctgaa
    10501 tgttattccc caagaaaccc aaaatggtgc caagcaactt tataaattca tttccagcaa
    10561 ctttactttt ccgttaatgg cagggcttgg tctgctttat atcccattaa aagaggttgt
    10621 ggcaaccctt agctggcaat attttattgt ggtgattagt gtggtgttaa cggttgtcaa
    10681 tgtcggtttt tttgtctctc gtttccttaa catgaactca gtggaagcag ctattatttc
    10741 agcttgtcaa agtggtatgg gtggaacagg tgatgtggct attttgagta cggctgaccg
    10801 catgaatcta atgccgtttg cccaagtcgc tacccgtcta ggtggggcta ttaccgttat
    10861 cactatgaca gctattttaa gaattatttt tagttaatcc aaaccaataa aaagagaggt
    10921 atttcctatg aaaaatcaat taggtcaact tgcccttgaa caagccaaaa cgttcggcgg
    10981 taaattggaa gttcaaccaa aagttgacat taaaactaaa catgatttga gcattgccta
    11041 cacaccaggt gtagcgagtg tgtcatctgc catcgcaaaa gataaaacat tagcatatga
    11101 cttaaccacc aagaaaaata cagtagccgt tatcagtgat ggtactgcgg ttcttggtct
    11161 gggggatatt ggccctgagg cagccatgcc agttatggaa ggaaaagcag ccctttttaa
    11221 agcatttgcg ggtgtcgatg ctattccgat tgtattagat accaaagata ctgaagaaat
    11281 tattagtatt gtcaaggcgc ttgcaccaac ctttggtggt attaatttag aggatattag
    11341 tgcgccccgc tgttttgaaa ttgagcagcg cttgattaag gaatgtcata ttcctgtttt
    11401 ccatgacgat cagcatggaa cagctattgt ggtgttagca gccatcttca atagtttgaa
    11461 acttcttaaa aaatcccttg atgaggtatc cattgtggtg aatggtggtg gatctgctgg
    11521 attattcatt acccgtaaat tattagcagc aggtgctacg aaagtgacag tagtggataa
    11581 atttggcatt atcaatgaac aagaagccgc tcaattagcg ccacatcatt tggatatagc
    11641 gaaagtcact aatcgtgagt ttaaatcagg taccttagaa gatgctcttg aaggagcaga
    11701 tatcttcatt ggcgtatcag caccaggagt cttaaaagca gagtggatta gtaaaatggc
    11761 agcgcgccca gtaatttttg ccatggccaa ccctattcca gagatttacc ctgatgaagc
    11821 ccttgaagcg ggggcttaca ttgtaggtac tggccgcagt gattttccta accagattaa
    11881 taatgtcctt gcctttccag gtatttttag gggagcatta gatgcacggg caaaaaccat
    11941 tactgttgag atgcaaattg ctgccgcaaa aggtattgcc agtcttgtcc cagatgacgc
    12001 tttgagcact actaatatta ttccagatgc ctttaaagaa ggagtcgctg agattgtcgc
    12061 taaaagcgtc aggtcggtag tccttaaatc atagaatata tgaaaaaaag agaaaattat
    12121 actttaaatc acaaaataag tagaagcata tttctagctt ctacttattt tgtgattagc
    12181 taatatcatt atcaataata actttcaagg cgttatttgc accagcatgt ttaaaggttt
    12241 cataagcttt ttcaacttct gaaagtttaa aatgatgtgt aataagtctt gttgcgtcaa
    12301 tctttccagt tttcaagaca tttagtagca tttcagttgt attggcatta acaagtccag
    12361 tgtttaaggt gatattttta atccataatt catccaggtt gaaatcaact ggtttcccat
    12421 gaaccccaac gttagcaata tgcccaccta ctgatataat tttttgacaa atatcaaatg
    12481 tcgcgggata accaacacac tccatggaaa tatcgacacc acgtccattg gttatatcgt
    12541 caatgatagc cttgacttct tcactacttc ctgaacaaat cgtgtgtgtg gctccaaatg
    12601 ttttagctgc ttcaaggcga ttttgagata agtcgaccat aataatattg gctggagaga
    12661 aaaattgaac ggttagtagg gctgcgagtc ctactggacc ggcaccaaca atacaaacat
    12721 tatctccagg cttgacatgt gatggcaaga caccaatttc atatgaagtt ggtaaaatat
    12781 cagaaagcat gacaagtgcc tcatcatcaa tcgtatcagg agcatgatag agactaccat
    12841 ctgcatgtgg gatatggaca tattctgctt gtgttccatt aattaaatgg ccaagaatcc
    12901 aaccgccatc ttcacaatgt gacgaaaggc ctcgtttaca ataataacag gtatgacatg
    12961 atgtgacgca agaaataatc actttatctc cgattttaaa gttagtgacc gcatcaccga
    13021 cctccttaac aattccaatt ccttcatgac caaggattgt tccttcttta catgcgggaa
    13081 catccccacc taagatatgc aaatcagttc cacaaatagt ggtttttacc aactggacga
    13141 tagcatctgt aggctttata atgactggtt ttggtttgtc tatgagctgt agatttccgg
    13201 ttgaaaggta agttgctgct ttcatattta actccttata aggttgagag gtaatcaacc
    13261 aattcatttt tacacagata ttataatagt ttaagaaagg gttttcaata ttttttaaat
    13321 cgattatgat aattttttag atcatctttg ttgaataact tcatccttat tcagaattta
    13381 aagggtacta ttataagaat ttgaaaccgc tttacttaaa ccgtgtattt ttgtacaata
    13441 atgattaagg aggtttttag atgaaaaaag aatttaccag tatcctgttt acagttagtt
    13501 tttgtggaat catagcattg cctgtcgaag ctagtggccc taaagttcct tatacacaag
    13561 aaggtataac ggccatttca aatcaggcaa cagtaaaatt gatttctatt gctgatattg
    13621 cgagcagttt agaaggacaa aaacctataa cggttagttt tgatatcgat gatactttgt
    13681 tatttactag ccaatatttt cagtacggca aagaatatat aactccaggc tcatttgatt
    13741 ttttgcataa gcaaaaattt tgggacttgg tcgctaaacg gggagatcag gattctattc
    13801 caaaagagta tgcaaagcaa ctaatcgcaa tgcatcaaaa acgaggagat aaaattgtct
    13861 ttattactgg ccgaactcga ggatcgatgt gtaaaaaagg tgagattgat aaaacagcta
    13921 agtcactggc taaagatttt aagttagaca agccaatagc tatcaattat acaggagata
    13981 aagctgtgaa gccgtatcag tatgataaaa catattatat aaagaaaaac ggctcacaaa
    14041 ttcattacgg agatagtgat gaagatataa atgctgccaa agaggcaggt gcaagaccta
    14101 ttcgcatttt aagagctcct aattcaacca atcttccttt gccaaaagct ggaggatacg
    14161 gagaagaggt tttagaaaat tcggcttatt agtcaaatat ttgtgtaaga aaggaagtaa
    14221 cagtaatttc tttcatcacc ccactgagta accaatagcc cagtgggttt tttgggttac
    14281 agaaatcagt tctaaaaaag aatcactggt aacttaaggt tgtctttgat acaatggtaa
    14341 cagtgtattt aaaagaaaga gaggacaaca tggaaaacca taaaaatgaa ttcacctttt
    14401 ccaacaaatc aattattgcc tatgtttggc gtggtgttgt ggttggtatt attgctggtg
    14461 ttattgttag tttattccgg ttgttaattg aagtgacagc tgactgggtg attgaatggt
    14521 accgatacgc tcatatcaat tcgctgttgc tacttcctat actaagtgtc agtttattag
    14581 ctgttctttt tgtaggtttt ttagtaaagt ctgattctga tataaaagga tcagggatcc
    14641 cccatgtcga aggagaacta aagggactaa tgtccccaga ttggtggagt gtcctttgga
    14701 aaaaattttt aggaggaatc atggctattt ctatgggatt tatgctcggt cgtgagggtc
    14761 cttcgattca gttaggtgct atgtcagcca aaggattagc taagttttta aaatcgagtc
    14821 gtttggaaaa gcgagtgcta attgccagcg gagcagcagc agggctatca gcagctttta
    14881 atgctccaat agcaggcctc ttgtttgttg ttgaagaaat ttaccatcat ttttcgcgtt
    14941 taatttggat tacagcgcta gtggctagct tagtggctaa ttttatttct ctgaatattt
    15001 ttggattgaa accagtctta gcaatgtcag aggcgatgcc ttttttagga ctcaatcaat
    15061 attggttgtt gcttttactt ggattatttc taggctgctt aggctattta tatgagatag
    15121 ttatccttaa ttttaataaa ctttatgtta ttttagggag ttggttacat ctgccagctt
    15181 atttttacgg tattattatg gtttttctaa ttttaccgat tggctattat ttaccacagt
    15241 tacttggagg aggacatggt ttaatacttt ctttatccaa tcaacaattg cctttgatga
    15301 ctatcttctt ttattttatt attcgattta ttgttagtat gtttagttat ggaagtggct
    15361 tacctggagg tattttttta cctattttaa ctttaggtgc tttggcaggc ctattatttg
    15421 ggcaaatagc ttctcaatta ggtttattga accaatcttt tctttctctt tttttgatat
    15481 tagggatggc agggtatttt gcggcaattt caaaagcacc actgacaggc atgattttgg
    15541 taactgaaat ggtaggtgat ttgaaaccct taatggccat agctgtagtt acttttgtct
    15601 cctatttagt gatggatttg ttaaatggac agccaattta cgaagctatg ctagataaga
    15661 tggcaatgaa gcatccaact aatcttgttg aaccaacatt aattgaattg actgttggtg
    15721 ataagattgc agggaaatat gttaaagagt taaagcttcc agaaaatgtc ttaattacaa
    15781 cccaaattca ccatcaaaaa tctcaagttg tgtcaggtaa cacgcgcctt ttgtcaggag
    15841 caactatctt tttggtcgtt aatgaagctg atacaggttt tgtccgtgag gtattgatgt
    15901 aggtattaat ttatcacaat tcggaaagtt cgagcttgtc ttttagacta agtcttcgaa
    15961 cgttttactg ctagagtttc atctgaacca aagtgaaacg cttttgatga agtcaagtag
    16021 gctttttgtt atactaacaa taaagataag ctaaggaggc tgagatgtta gaaattgttt
    16081 cagaagagct acgtcactat caagaacaaa aattgattga gtaccgtaac aaaaatcagt
    16141 tagccccaaa aggaggtatt gtatttgcag gtgattctct cattgaattt tttcctttaa
    16201 aaaaagcttt tggttcatgt ttgcctatta ttaatcgagg tattgctggg atagatagtc
    16261 agtggttatt gcgccatttt tctgtccaaa ttactgattt agagcctaaa catatttttt
    16321 tattaattgg ttgtaatgat atcgggcttg gatatgataa atgtcatatt gtcaagacta
    16381 ttgtagagct tattagccag attcgtagtc attgtgtcta cagtcagatt tatctcttat
    16441 ctttattacc agtttccaac aatccccgat atcagaaaac tgtcaaaata agaacaaatg
    16501 ctatgattga tgctattaat aaagatttag caatgattcc aactatagaa tttattaatc
    16561 ttaatacttg tcttaaagac gaaaaaggtg gtttatcaga tgagaatact ctagatggtt
    16621 tacatcttaa ttttccagct tatgctaaac tagctgaaat tattcaatct tatatctagc
    16681 caataaatgt actaatagcg atgaggcaag aagtcagata gcagacggtt catcaatcaa
    16741 ttaagaaaaa agttatcaag ataccattaa aaatagttgc tcaaagttct ttcaaatcaa
    16801 aagattatga tatcgctatg ttgagtttta gtgtataatg ataattaaag gagttaccat
    16861 gaaatttgaa aaaaaacaag tcttctattt agtccttacg tttatcttat gctatgggat
    16921 cctagcaaac tggcgaaatg gaacggctat cgtcacgact atttacaaaa caagccttcc
    16981 ttttttttat ggagcagcag gtgcctatat tgttaatatt gtaatgagcg cttacgaaaa
    17041 ggtatatgtt tatatcttta aagactggtc acatgtctta aaagtaaaac ggggtatttg
    17101 tttactgtta gcgtatttga cattttttat tttaattact tggattattt ccattgtcat
    17161 tccagactta attacaagta ttagtactct aacaaaattt gatactataa cgatacaaga
    17221 agttgttaac aatcttgagc acaataaact gttagcacgt actattcagt atattggtgg
    17281 cgatggcaaa cttacagaga cgattgctaa ctatagtcaa cagttattaa agcaatttct
    17341 tactgttttg acaaatattt taacatctgt cacggttatt gcttctgcaa ttattaatct
    17401 ttttattagt tttgtttttt cgttatacgt tttagcaagt aaagaagatc tttgtcgtca
    17461 gggaaatact ttagtagata cttatactgg taagtacgct aaacgcattc attacttgtt
    17521 agagttgttg catcagcgtt ttcacggatt ttttgttagt cagaccttag aagctatgat
    17581 tttaggttcg ctgactgcta gtggcatgtt tatattaaga ttaccatttg cagggactat
    17641 tggcgtttta gtagctttta cagcattgat tccagttata ggtgcttcaa ttggagcagc
    17701 tattggattt attttaatta tgactcagtc catgtcacag gccattattt ttatcatttt
    17761 tttaattatt ttgcagcaga ttgaaggcaa ttttatttat ccgaaagtag ttggtggatc
    17821 gattggatta ccggctatgt gggtattaat ggcaattaca ataggcgctt ctttaaaggg
    17881 aatagttggt atgattattg cagttccttt agcagcgaca ctttatcaag tgattaaaga
    17941 taatattcaa aaaagacaag ctattcaaaa aaaacaagtt tcctaaaagg aacttgtttt
    18001 tttgtcgaat aaaaagatgg aggtaatatg ataaatgtat tctataaaat gtgatgataa
    18061 taaagccatg ccaagagaac gtttgatgcg actaggagca gagtctctaa gtaatcaaga
    18121 attattagca attttattac gaacaggtaa taaagaaaag catgtcttag agctgtcatc
    18181 ctatctttta tcgcatttag acagtctggc agattttaaa aagatgtctt tgcaagaatt
    18241 acaacatttg gcaggtatag gaaaagttaa agcgattgaa attaaagcta tgattgagtt
    18301 ggtttcccga attttagcga cagataagac attaactgat agcgtattaa ccagtgttca
    18361 ggtcgctgaa aaaatgatgg cagctttagg agataaaaaa caagagcatt tagtcgtatt
    18421 gtatttagat aatcaaaatc gtattattga agaaaaaact atttttattg ggactgtccg
    18481 acgttcactt gcagaaccaa gagaaatttt atactatgcc tgtaaaaata tggcgactag
    18541 tctcattgtt attcataatc atccttcagg aaatattgaa cctagttcta acgattattg
    18601 ctttactgaa aaaataaaac gatcatgtga agatttaggc attatctgtc tagatcacat
    18661 tatcgttagc tataaagatt attatagttt tcgagaaaaa tcaacccttt tttaagtcag
    18721 aagtcattga cgaataagcg gaacaattgt agatcttcat ctcgaacacc ttggagaagt
    18781 tcaggatgcc actgaacacc taaaaaagga atggcatcgt tggttgatat gactgcttcg
    18841 atggtaccat cacgtggatc tctagcgatt acttttaaat ccttagcaac agtttttaaa
    18901 ctttggcgat gaaaagaatt aattaatgtc ttatgaccat aaataggata taagatagag
    18961 tctggctcaa taatcatttc gtgtgataag aaatcagaag gcgcttcttg ccaatgtgag
    19021 tcaatgtgtt ggttaagatt tcctcctaag gcaacattca ttaactgtgt accacgacaa
    19081 atccctaaga ttggtttttt taatgttata gcctccttga tgatagctag ctcaaatgtg
    19141 tctcgctctg gtgaaaaatc atcatcaaaa gcagccttct cttcttgata atattttggg
    19201 tcaacgtttt gcccaccaat taaaatgatt ttatcaacca tagatacata agttttagcg
    19261 gcggcttcat caccaatggg taaaagaagc ggcagccccc cagattgagt gactgcctgt
    19321 acaaatccag taggtgcata tgaccatggg agattatcca aagccatatt tagtctctga
    19381 tttgcagtta ttccaataat cggtttggtc attatacctc cttaatctag atgattttga
    19441 ttcataaaat agagtagtgt ctggagctca ctggtcaaat cgacagattg aacaataact
    19501 cctttaggaa cttgtaggtg aacaggagca aaactcaaga ttcctttgat tcctgcttca
    19561 ataagttgat cagtaacttc ttgagcatga atgctgggaa ccgttaagat agccgtttca
    19621 atgtcagtgt tcgcaatccg ttctttgaca gatgagatac catggactgg aatattatca
    19681 gctgttttag taccaacaag agcgttatca tcggtatcaa aacccatggc aatttgcatc
    19741 ttattacgat catggaagcg gtaatgtaat agagcacgcc ctatattacc gcatccaact
    19801 aagataacgt ttgttgtaga gtgatcatta agcaagtcag caaaaaaatt catcaatttg
    19861 gtaacatcat agccaaaccc acgacgcccc aattctccaa aataagaaaa gtcacggcgt
    19921 acagtagcag agtcaattcc cattgcatct gcaatttgtt tagaactggc tttttcaact
    19981 tggtcagcat ggaatcgttt aaaaatacgg taataaagtg ataagcgctt tgcagtcgct
    20041 tttggaatag atttgtcaat aaccactatt ttactccttg tagatttctc gttaaatatg
    20101 tctgtcttta ttgtaacaaa aagatt
//
LOCUS       DAUCWU010000023        19778 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUCWU010000023 DAUCWU010000000
VERSION     DAUCWU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.023"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            48..1055
                     /gene="plsX"
                     /locus_tag="VGY24_000794"
     CDS             48..1055
                     /gene="plsX"
                     /locus_tag="VGY24_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ4283794.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKAEAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            1048..1290
                     /locus_tag="VGY24_000795"
     CDS             1048..1290
                     /locus_tag="VGY24_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ4283795.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            1442..2146
                     /locus_tag="VGY24_000796"
     CDS             1442..2146
                     /locus_tag="VGY24_000796"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ4283796.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVATHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     FKCWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            2271..5996
                     /locus_tag="VGY24_000797"
     CDS             2271..5996
                     /locus_tag="VGY24_000797"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ4283797.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDRLLTEAEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYQADDFATYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAEIHAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLVKKSNDF
                     GAGGICVAIGELADGLEIDLDKAPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSIGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIAGVVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANANSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQPLFASAVKYFTGK"
     gene            6230..7684
                     /locus_tag="VGY24_000798"
     CDS             6230..7684
                     /locus_tag="VGY24_000798"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ4283798.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            7712..8734
                     /locus_tag="VGY24_000799"
     CDS             7712..8734
                     /locus_tag="VGY24_000799"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ4283799.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVIERIKKHVARTERAGVMGVLGGFG
                     GMFDLSKTGVKEPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGQDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     ASVVIK"
     gene            8902..9456
                     /gene="purN"
                     /locus_tag="VGY24_000800"
     CDS             8902..9456
                     /gene="purN"
                     /locus_tag="VGY24_000800"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ4283800.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQVIVDLLDKHEIDLVCLAGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            9640..11187
                     /gene="purH"
                     /locus_tag="VGY24_000801"
     CDS             9640..11187
                     /gene="purH"
                     /locus_tag="VGY24_000801"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ4283801.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIEAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(11245..12369)
                     /locus_tag="VGY24_000802"
     CDS             complement(11245..12369)
                     /locus_tag="VGY24_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ4283802.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFIVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            12622..13887
                     /gene="purD"
                     /locus_tag="VGY24_000803"
     CDS             12622..13887
                     /gene="purD"
                     /locus_tag="VGY24_000803"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ4283803.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNAPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPFDYEKGVPLPEKTDGDIITYYAGAKLAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            14165..14656
                     /gene="purE"
                     /locus_tag="VGY24_000804"
     CDS             14165..14656
                     /gene="purE"
                     /locus_tag="VGY24_000804"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ4283804.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRVLSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            14643..15716
                     /gene="purK"
                     /locus_tag="VGY24_000805"
     CDS             14643..15716
                     /gene="purK"
                     /locus_tag="VGY24_000805"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ4283805.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPTNDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTIAPYKVVTSSLDLEGLDFTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKTAVQIAKKLQLSGTLCVEMFTTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPQIQLHAPAVMLNVLGQHVQQATDYVAKNPSAHLHMYGKLE
                     AKHNRKMGHVTVFAKDADEVKEF"
     gene            15743..17440
                     /locus_tag="VGY24_000806"
                     /pseudo
     CDS             15743..17440
                     /locus_tag="VGY24_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
     gene            17458..18750
                     /gene="purB"
                     /locus_tag="VGY24_000807"
     CDS             17458..18750
                     /gene="purB"
                     /locus_tag="VGY24_000807"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ4283806.1"
                     /translation="MLERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRIFEIEQNTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
     gene            18881..>19778
                     /gene="rgg4"
                     /locus_tag="VGY24_000808"
     CDS             18881..>19778
                     /gene="rgg4"
                     /locus_tag="VGY24_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEQ4283807.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEGMDGQYSDKIFYDSLLDNLSEQFELDELFIVNKIIIDISSLSLK
                     NNRLDNLEKAIEMSQKIMAKIQDWNRMPILKLIEWKYFLIKQKDIIKAEQSFMKACLF
                     AQMTADQYLENKLIQEWEKDVKS"
BASE COUNT         5989 a         3613 c         4493 g         5683 t
ORIGIN      
        1 tattaataga ttgtgtaaga acacaagata taattaaagg agtggaaatg aagagaattg
       61 caatagatgc tatgggtgga gataacgctc ctaaagctat tgttgagggt gttaatcaag
      121 caatagaagc attttcagat atcgaaattc aactttacgg tgatcagaca aagattaact
      181 cctatcttat tcaatcagat cgtgttgcta ttattcatac agatgaaaaa attatgtcag
      241 atgatgaacc tgcaaaagct gttcgccgta aaaagaaggc ttcaatggtt ttagcagcta
      301 aggcagtgaa agaaggaaaa gcagaggcca ttatttcagc agggaataca ggtgctcttt
      361 tagctgtagg tttgtttgtt gtggggcgta tcaagggagt tgatcgtcca ggattattat
      421 caactattcc cacagtaacc ggattaggat ttgatatgtt ggatcttgga gctaatgctg
      481 aaaatacggc aaaacattta catcaatatg ctattttagg ctctttctat gctaagaatg
      541 tacgtgggat tgctaatcca cgtgttggtc tattaaacaa tggtacagaa gaaacaaaag
      601 gagatcccct tcgtaaggcc acttatgaac ttttaacagc agataataca atatcctttg
      661 ttggtaatgt cgaggcgcga gaacttatgt caggtgttgc agatgttatt gtatcagatg
      721 gtttcacggg aaacgcagtg ttgaaatcaa tagaaggaac agctattagc attatggggc
      781 aactgaaaca gataattaat agcggcggta ttaagactaa aattggagct agtctactta
      841 aaagtagtct ttacgagatg aagaagactc ttgactactc tagtgctgga ggtgcagttt
      901 tatttggttt aaaggcacct gttgtaaagt ctcatggttc tagtgatgta aaagctattt
      961 tttcaacgat aaaacaagta cgtacgatgc tagacactaa cgtggtcgga caattagtag
     1021 aagaatttgc taaggagact caagtaaatg actagacagg agatttttga aaggctgatt
     1081 aacttaattc agaagcaaag aagctatttg agtgtagcta ttacagaaca aacacattta
     1141 aaaaatgatt tagcggttga ctctattgaa ctagttgaat ttattattaa tgttgaagat
     1201 gaatttcata ttgctattcc tgatgaagat gttgaggata tggtatttat gggagacgtt
     1261 ttagattatt tagttcagcg tttgacttag ttagaataat aaaaagagcg taagctcttt
     1321 ttattattct ttaaaaacga accttttttt taaaaaaact tataaacgta aacaatatct
     1381 tgaatatttt tatagagtat gttactctta aatgaaaaca gaaagaaagg cgaataatca
     1441 agtgactaac cagcttattt ataaaggaaa agcgaaagat atttatagta caaaagatga
     1501 aaatgtgatt agaacggtct ataaagacca agctactatg ttgaatggag ctcgtaagga
     1561 gactattgat ggcaagggag cactaaataa tcagatttca tctcttattt ttgagaaatt
     1621 aaataaggct ggtgtggcga cacattatat tgagcaaatt tcaaaaaacg aacaattgaa
     1681 taaaaaagtt gatattattc cacttgaggt ggtgttacgg aatgtgacag caggctcatt
     1741 ttcaaaacgt tttggagtgg aagaaggacg tgttcttgaa acgccaattg tggaatttta
     1801 ctacaaaaat gatgatctga atgatccttt tatcaatgat gagcatgtga agtttttggg
     1861 tattgttaat gatgaggaga ttgcttatct gaaaggagag actcgccgta ttaatgaact
     1921 cttcaaatgt tggtttgctc agattgggct taacttgatt gactttaagt tggagttcgg
     1981 atttgatcaa gaaggtacaa ttatcttagc agatgaattt tcgcctgata actgtcgcct
     2041 atgggataag aatggtaacc atatggacaa ggatgttttt agaagagacc ttggcaacct
     2101 gacggatgtc tatcaagttg tacttgagaa gttgatagca ttgtaactag aagaggaagg
     2161 aattgaatcc gttcgggaaa ctccgaaaaa aaagatggaa cttactgaaa tctcaatttc
     2221 agatgtcctc ctattttcca gtcgctccct tgtcggactt ggtatcttat atgaataaac
     2281 gtatttttgt tgagaaaaag gctgactttg gcattaaatc ggctagcctt gtgaaagagt
     2341 tgacacataa tctacaactg acttctttga aggacttgcg tattgtgcag gtctatgatg
     2401 tcttcaattt ggctgaggat ttactggcgc gtgctgagaa gcatattttc tctgagcagg
     2461 tgacagaccg tcttttgacg gaagctgaaa tcactgcgga gcttgataag gttgccttct
     2521 ttgccattga ggcgcttcct ggtcaatttg accaacgtgc tgctagttcg caagaagctt
     2581 tgctattatt tggaagtgac agtcaggtta aggtcaatac agcccagctt tacttggtca
     2641 ataaggatat tacagaagca gagcttgaag ccgttaagaa ctatcttttg aaccctgttg
     2701 attcgcgttt caaggacatt actttgccgc ttgaagagca ggctttctct gtgtctgata
     2761 agacgatccc taatcttgat ttctttgaaa cttatcaagc tgacgatttt gcgacttata
     2821 aggcagagca gggcttggct atggaggtcg atgaccttct cttcatccaa gattatttca
     2881 aatcaatcgg acgtgtgcca actgagactg agttgaaagt tttggatact tactggtcag
     2941 accactgccg tcacacaacc tttgaaactg aattgaagaa cattgatttt tcagcttcta
     3001 aattccaaaa acaattgcag acaacttatg acaaatatat cgccatgcgt gatgagcttg
     3061 gtcgttctga aaagccacaa acacttatgg atatggcgac tatttttggt cgttatgagc
     3121 gtgccaacgg tcgtctggac gatatggaag tctcagatga aatcaatgcc tgctcagttg
     3181 agattgaagt agatgttgat ggtgtgaaag agccttggct cctcatgttt aagaacgaga
     3241 ctcacaatca cccaacagaa attgagccat ttggtggagc ggcgacttgt atcggtggtg
     3301 ctattcgtga cccattgtca gggcgttcat acgtttatca ggctatgcgt atttcaggcg
     3361 caggcgatat cacgactccg attgcggaaa tacatgctgg taaattgcca caacaagtta
     3421 tttctaaaac tgcggcgcac ggctattctt catatggtaa ccaaattggg cttgcgacaa
     3481 cttatgtgcg tgagtatttc caccctggct ttgtagccaa acgtatggag cttggagctg
     3541 tggttggtgc tgcacctaag gaaaatgtgg ttcgtgaaaa accagaagca ggtgatgtgg
     3601 tcatcttgct cggtggtaaa acaggtcgtg atggtgtcgg tggtgcgaca ggttcatcta
     3661 aggttcaaac ggttgaatct gtggaaacag ctggcgcaga ggtacaaaaa gggaatgcca
     3721 ttgaagaacg taagattcaa cgtcttttcc gtgatggcaa tgtcactcgt cttgttaaga
     3781 aatcaaatga cttcggtgca ggtggtatct gtgttgccat cggtgaattg gctgacggtc
     3841 ttgaaatcga tttggacaag gcgcctctta aataccaagg tcttaatggt actgaaattg
     3901 caatctcaga atctcaagag cgtatgtcag tcgttgttcg tccaagtgat gtggatgcct
     3961 tcatcgcagc ctgcaacaag gaaaatatcg atgcagtcgt agttgcgacc gttactgaaa
     4021 aaccaaatct tgtcatgact tggaatggcg aaatcatcgt tgatttggaa cgccgtttcc
     4081 ttgataccaa tggtgtccgt gtcgttgttg atgctaaagt cgttgacaag gacttgacag
     4141 ttccagaagc acgtacaaca tcagcagaga cacttgaagc agatacgctt aaggtcttgt
     4201 ctgacctcaa ccacgctagt caaaaaggtc tccaaaccat ctttgactca tctattggtc
     4261 gttcaaccgt taaccaccca atcggtggtc gttaccaaat cacaccgaca gaaagttctg
     4321 ttcaaaaatt gccagttcaa catggtgtga caacaactgc atctgttatg gctcaaggtt
     4381 acaatcctta tattgcagag tggtcacctt atcacggtgc tgcctatgct gttattgaag
     4441 cgacggctcg tttggttgca acaggtgctg actggtctcg tgcacgtttc tcttaccaag
     4501 agtactttga gcgtatggat aaacaggcag agcgttttgg tcagccagta tcagctcttc
     4561 ttggttctat tgaggctcag attcaacttg gtttgccatc aatcggcggt aaggactcta
     4621 tgtctggtac tttcgaagac ttgacagtac caccaacctt ggtagctttc ggcgtgacaa
     4681 cagcggacag ccgcaaggtt ctctctcctg agtttaaagc ggctggcgaa aacatttact
     4741 atatcccagg tcaagctatt tcagaagata ttgattttga ccttatcaag gctaacttta
     4801 gccagtttga agctattcaa gctcaacata agattacagc tgcctcagcc gctaaatacg
     4861 gtggtgtcct agaaagtctt gctctcatga cttttggtaa ccgtatcggt gcttctgttg
     4921 aaattgcaga gcttgacagc agcttgacag ctcaactcgg aggttttgtc tttacatcag
     4981 ctgaggaaat tgctggtgtg gtgaaaatcg gtcaaactca ggcagacttt acagtcactg
     5041 tcaatggaaa tgacctagct ggcgctagcc ttctagcagc cttcgaaggc aaattggaag
     5101 aggtttaccc aacagaattc gaacaggcag acgctcttga agaagttcct gctgtggtat
     5161 cagatactgt tatcaaggct aaggaaacaa ttgaaaaacc agtggtttac attcctgtct
     5221 tccctggtac caactcagaa tatgattcag ctaaggcctt tgaacaggtt ggagctagtg
     5281 tcaacttggt accatttgta accttgaatg aggttgctat tgctgagtca gttgacacta
     5341 tggttgctaa tattgctaag gcaaatatca tcttctttgc tggaggcttc tcagcagcgg
     5401 atgaaccaga tgggtctgct aagtttatcg tcaatatctt gcttaacgag aaggtccgcg
     5461 cagctattga cagcttcatc gaaaaaggtg gccttatcat aggtatctgt aatggtttcc
     5521 aagcccttgt taaatcaggt cttcttccat acggaaactt cgaggaagct ggtgagacaa
     5581 gtccaactct cttttataat gatgcgaacc aacacgttgc caagatggtt gagactcgta
     5641 tcgcaaatgc caactcacct tggttggcag gagttgaggt cggcgatatt cacgccattc
     5701 cagtttcaca cggtgaaggt aaatttgttg tcagcgcttc tgaatttgca gagctaagag
     5761 acaatggtca aatctggagc caatatgtgg actttgacgg acaaccatct atggattcta
     5821 aatacaatcc aaacggctct gtcaatgcca tcgaagggat taccagcaag aatggtcaaa
     5881 tcatcggtaa gatgggacac tcagaacgct gggaagacgg actcttccaa aatatccctg
     5941 gtaacaaaga ccaacccctc tttgcaagtg ctgtaaaata ctttacagga aaataatttt
     6001 ttcaaatttt ttcataaaac agataaggaa acaagagcga agcgagctta actgtatctt
     6061 tctgtcgtag tggtatttgg gagccgttga ggctaagact caatgattag cttgaaaata
     6121 gcttgataga ttttctattt tgatgttggc attggtgacg atagatccaa aattgagaaa
     6181 atcatcaaag ctacttaaga agagatacaa aaagaattag gaaaaaagaa tgacatacga
     6241 agtaaaatct ctaaatgaag aatgtggagt ctttggtatc tggggacatc ctcaggcagc
     6301 tcaagtcact tactttgggc ttcatagcct tcaacatcgc ggtcaagaag gagctggtat
     6361 tgtttcaaat gacaatggga aactctatgg ctatcgaaat gttgggctcc tttctgaagt
     6421 ttttaagaat caatctgaat tagataattt aactgggaat gcggctattg gacatgttcg
     6481 gtatgctact gcaggttctg cagatattcg caatattcag ccttttcttt ataaatttca
     6541 tgacgggcaa tttgctttat gccataatgg taatttgaca aatgctattt ccttaaggaa
     6601 agaattagaa aagcaaggtg caattttcaa tgcctcctca gatactgaaa ttttgatgca
     6661 cttgattcgt cgaagccata actcaagttt catgggaaag gtaaaagaag ctctaaatac
     6721 agtaaagggt ggctttgcct atctactgat gacagaaaat aaactcattg ctgctcttga
     6781 ccctaatgcc tttcgtcctt tgtcaattgg acaaatgcaa aatggtgcct gggttatttc
     6841 cagtgagacc tgtgcttttg aggtggtagg cgcaaaatgg gttagagatg ttgaacctgg
     6901 cgaagttatt cttatagacg atcgtggtat tcaatgtgat cgttatactg atgaaacgca
     6961 actggcaatt tgttcgatgg aatatgtcta ttttgcaagg ccggattcaa ctatacatgg
     7021 tgttaatgtt catacggctc gaaaaaatat gggaaagcgt cttgcacaag aatttaaaca
     7081 ggatgctgat attgtaattg gtgtcccaaa ttcgtcctta tcggctgcta tgggctttgc
     7141 tgaagaatcc ggattaccaa atgagatggg tcttgtaaaa aatcagtata cgcagcgaac
     7201 ctttattcaa ccgacacaag aattaaggga acaaggtgtt cgaatgaaac tatcagcggt
     7261 atctggtgtt gtcaaaggaa agcgcgttgt tatgattgat gactcaattg taagaggaac
     7321 gacttctaga aggattgtcg gattattaag agaagcagga gctagtgaag tacatgttgc
     7381 tatagctagt ccagaattaa agtatccttg tttttatggt attgatattc agactcgtcg
     7441 tgagttgatt tcagcaaatc acgctgttga tgaagtgtgt gatattattg gcgcagatag
     7501 tctgacctat ctctcactgg atgggttgat tgaatcaatt ggactcgaaa caaaagcacc
     7561 aaatggtggt ttatgcgtag cttactttga tgggcattat ccaacaccgc tttatgatta
     7621 tgaagaagaa tacctcagaa gtttagaaga gaaaacaagt ttttatattc aaaaagtgaa
     7681 ataagctctg ctaattatat aaggagaaaa catgtctgaa aaaaatgctt atgcaaaatc
     7741 tggtgttgat gttgaagctg gttatgaagt gattgaaaga ataaaaaaac acgttgctcg
     7801 tacagaacgt gcgggagtta tgggagttct aggtggcttt ggtgggatgt ttgacttgag
     7861 taaaacagga gttaaagagc ctgtcttggt ttcagggact gacggtgtcg gaacaaaact
     7921 tatgcttgct atcaagtacg acaagcacga cacaatcggt caagactgtg ttgccatgtg
     7981 tgtcaatgat attattgcag caggtgctga gcccctttac ttccttgact acgtagcgac
     8041 aggaaaaaat aatccagtca aatttgaaga agttgtttct ggcgttgcag aaggttgtgt
     8101 tcaagcagga gcagcactca ttggaggtga gaccgctgaa atgcctggaa tgtatggtca
     8161 agatgattat gaccttgcag gctttgctgt tggtgtggct gaaaaatctc aaatcatcga
     8221 cggttcaaaa gtaaaagaag gcgatattct tttgggactt gcttcaagtg gtatccattc
     8281 aaacggttat tctttggtac gtcgtgtctt tgctgactat actggtaaag agctgcttcc
     8341 agagcttgaa ggcaaacaac ttaaggatgt ccttcttgag ccaactcgta tctatgttaa
     8401 agcagctctg ccattaatca aggaagaact agtcaacggt atcggccaca tcacaggtgg
     8461 tggctttatc gagaatgttc ctcgtatgtt tgcagatgat ttggctgcgg aaatcgatga
     8521 ggacaaggtg ccagtgcttc cgattttcaa ggcgcttgaa aaatatggtg acatcaagca
     8581 cgaagaaatg tttgaaatct tcaatatggg tgtcggtctt atgctagctg ttagtcctga
     8641 aaatgttaac cgcgtcaaag agctcttgga tgaaccagtt tatgaaatcg gtcgtatcat
     8701 caagaaagca gacgctagtg tggtgattaa ataagtcact aaattttata agaaaggagt
     8761 gtttagttag tttgcctaac tgaacacggg attaaatttc taggaaaaaa gataaaatga
     8821 cttagacgca ggcgtcgttg ttcgttttcc tatttttcta cgaaattttt cggcaagccg
     8881 aatcatccct ttgtatcgaa tatgaaaatc gctgtttttg cttctggtaa tggttccaac
     8941 tttcaggtca tagcagagca gtttccagtt agttttgtct tttcagatca tcgtgatgcc
     9001 tatgttttag agcgtgctca gaatttagcc attccaagct tcgcttttga actcaaggag
     9061 tttgagaata aggcggctta cgagcaagtc atcgttgatt tgttggacaa acacgagatt
     9121 gatttggtct gtctggcggg ctatatgaag attgttggag aaaccttact cttggcctat
     9181 gaggggcgta ttatcaatat tcacccagcc tacctgcctg aatttccagg tgcccacggt
     9241 atcgaggatg cttgggaagc tggtgttgac cagtctggcg tgaccatcca ctgggttgac
     9301 tctggtgtgg ataccggtca ggtcatccaa caagtgcgcg tgccacgcct agcagacgat
     9361 agcctagaaa gctttgaaac ccgtatccat gaaaccgaat atcaactcta cccagctgtc
     9421 ttagatagct tgggagtgga gaggaaagta atctagcctt tatcttaaaa ttttaggtaa
     9481 agttaggaaa gatagtaaag aagaattaac gttccaaaaa gaaaggaatt gaactcgtct
     9541 ggaaggcaca gaaaaaagat aaacatctcc taggcgcaag cgtcttcgtc gagtttccta
     9601 tttttccttg gccttctagt cggacttggt atcttaaata tgactaaacg tgctttaatc
     9661 tcagtgtctg acaagtcagg aattgttgac tttgcaaaag aattgaaaaa cttgggttgg
     9721 gatattatct caactggtgg gactaaggtt gcccttgacg atgctggtgt tgagaccatt
     9781 gccatcgacg atgtgactgg attcccagag atgatggacg gtcgtgttaa gaccctccac
     9841 ccaaacattc acggtgggct tttggctcgt cgcgacgctg acagccacct tcaggctgct
     9901 aaggacaaca atatcgagct gattgacctc gtggttgtca acctctatcc cttcaaggag
     9961 actatccttc gtccagacat aacctatgat ttggcggtgg aaaacatcga catcggcggt
    10021 ccatcaatgc ttcgctcagc tgctaaaaat cacgctagcg tgaccgttgt ggttgaccca
    10081 gcggactatg cgactgtttt gggagaattg gctgacgctg gtcagacgac atttgaaacg
    10141 cgtcaacgct tggcagctaa ggtctttcgt cacacagcag cctacgacgc tttgattgct
    10201 gagtacttca caactcaagt gggagaggct aagcctgaaa aattaaccat cacttacgac
    10261 cttaaacagg ctatgcgtta cggagaaaat ccacaacaag acgctgattt ctatcaaaaa
    10321 gccttgccaa cagactactc aattgcttct gctaaacagc tcaacggaaa agaattgtcc
    10381 ttcaataata tccgtgatgc cgatgcagca atccgtatta tccgtgattt caaagaccgt
    10441 ccgacagtgg ttgccctcaa acacatgaac ccatgtggta tcggacaggc tgatgatatt
    10501 gagactgctt gggattacgc ttatgaagct gacccagttt caatctttgg tggaattgtc
    10561 gtccttaacc gtgaagttga cgcagcgaca gccaagaaga tgcatcctat ctttttggaa
    10621 atcatcatcg caccatcata ctcagaagaa gcgctagcta ttctcacaaa taaaaagaaa
    10681 aacttgcgaa tccttgagtt gccgtttgat gcccaagctg ccagcgaagt ggaagctgag
    10741 tacactggcg tagttggtgg acttttggtg caaaaccaag acgttgtggc tgaaaatcca
    10801 tctgactggc aagtggtgac agaccgccaa ccaacagaac aagaggcgac tgcccttgag
    10861 tttgcctgga aggctatcaa gtatgttaag tctaacggga ttattattac taacgatcac
    10921 atgacgcttg gactcggtgc aggtcaaacc aaccgtgtcg gctcagtcaa gattgctatc
    10981 gagcaggcta aagaccacct tgacggtgcc gttctagcat cagatgcctt cttcccattt
    11041 gcggacaaca ttgaagaaat cgctgccgca gggatcaaag caatcatcca accaggtggt
    11101 tcagttcgtg accaagactc tattgaggcc gcaaacaaac atggcttgac catgatcttc
    11161 acaggtgtga gacattttag gcattaataa aaaaatgtgg tctaattaaa accacatttt
    11221 ttattaaaag aaaatataag ttaactataa tttgttagct ttatcagcat ttacccatgt
    11281 tccacgagat ccgatttgaa cataaatacc attacttggt tgatcaattt ttaatacttt
    11341 ataactacca gtgacgataa aatattcacc cacacggaga atttgatctc ctaaaacttt
    11401 tccttggtta tctgtttcat ctaatggtgt gggatcaatc cagtttagtg gagttggatc
    11461 tcctccggct aattctttat taacgatcaa attattaaca agctgatcta cacgaaaaac
    11521 gccagggaag gtaacagtat caccgacctt aatggaaggt tttgtattgt cattttgaac
    11581 cacagactgt gctggctctt taatgggaat atagcgtcgg tttccagaaa aactgaggta
    11641 gctaagccat gtataacctc cagcagtcac gactttatcg taataaacag attgacctgc
    11701 ttcatagtaa gcaagatcag ggctatcgat actggtttgt cctttgactg gtaatctggt
    11761 tgtaaagtga taagttccag aggggtcaaa tgaagcttga gaaatgtgtt ttagttgtct
    11821 tgggtaggaa tgacttgtct gagatgataa gtctttaaaa tggatataac cacttacctg
    11881 agattttgga atttgacgct tatggtatct ttcagggcct tgtccagcgt tgtaattata
    11941 ctcttcgata gtgacagtgt ctccacggat atcagctacc catgctacat gaccgtaagc
    12001 agcatttgac tgataagcgt ttttatcaaa ccaagcgata gcccctatgc ttggtgtctt
    12061 attcacagga taaccctgat ttttcgcgat atgtccccac gtgcaggcat taccgtagcc
    12121 tttaggtaac tgaaaaccat tagcagagct taaacgaaaa gctgcaaaag aagtgcattg
    12181 gcggatatac atgttccacg aatcgattcc attgcctttt ttccacttac tcggatagtt
    12241 atcgcctaaa acagctgcat gaacaagatt ttcctgttgc gaaataagtg tagatggcat
    12301 agtaggtact agaccattaa aacctaaaag gattactcct gagaccaaaa aacgatgaaa
    12361 ttttttcatt aattacctcc tgtaacttat tattcgaaag caagaaacaa aaaatgttat
    12421 ttttagatga ttattttgaa aattggcttt acttgatatt aaataactgt ttgattggct
    12481 aatagagtgt tccttatttc aagaattgtg gtctactatc acatttttac aatgtttcaa
    12541 ttattgataa ctaatacatt atgtaaaaaa gcgaacgttt atgatataat atctaattat
    12601 aatacgaaag aggtggatgt tttgaaatta cttgttgttg gttcaggtgg tcgtgaacat
    12661 gcgattgcta agaagttgtt agcgtctaag ggtgtggatc aggtttttgt ggcacctggt
    12721 aatgatggta tgaccttgga tggtctggac ttggtgaata tagtagtttc cgaacattcc
    12781 aggttgattg cctttgctaa ggagaatgag atttcttggg cctttattgg tcctgatgat
    12841 gcgctagcag ctggtatcgt tgatgatttc aacagtgctg gactcagagc ttttggtcca
    12901 acaaaggcag cagcggagct agagtggtca aaagactttg ccaaggaaat catggtcaaa
    12961 tacaatgccc caacagcagc ctatggcaca ttttcagact tcgaaaaagc caaagcctac
    13021 atcgaagagc agggcgcacc tatcgtggtc aaggctgatg gcttggcgct aggtaagggt
    13081 gtggtcgtgg ctgaaaccgt tgagcaggcg gtagaggcgg cgcaagagat gcttctggac
    13141 aataagtttg gcgactcagg agcgcgtgtg gttatcgagg aattcttgga tggcgaggag
    13201 ttttccctct ttgcctttgt taatggtgat aagttctaca tcatgccgac agctcaggat
    13261 cacaagcgtg cctttgacgg agacaagggg ccaaacactg gtggtatggg tgcctatgcg
    13321 ccagttcctc atctacctca gagcgtggta gacacagcgg ttgagatgat cgttaggcct
    13381 gtccttgagg gcatggttgc cgaagggcgt ccttatctag gtgtcctcta tgtggggctt
    13441 atcctgacag ctgatggacc taaggttatc gagttcaact cacgtttcgg tgaccctgaa
    13501 actcagatta tcctccctcg cctgacttct gatttcgctc agaatattga cgatatcatg
    13561 atgggcattg agccttacat cacttggcag aaggacggtg tgactttggg tgttgtcgtt
    13621 gcctcagaag gctatccatt cgattatgag aaaggtgtgc cactgcctga aaagaccgac
    13681 ggcgacatca tcacctacta cgcgggagct aagcttgcgg aaaatagcga gctacttctc
    13741 tcaaacggag gacgtgtcta tatgctagtc accacagaag acagcgtcaa agcagggcag
    13801 gacaaaatct atacccaact agcccaacaa gacacaacag gtctcttcta ccgaaatgac
    13861 atcggaagca aagctataag agaataagac taatcattca aatattagcg tcagcaatca
    13921 gcgaccgaag gtcgctccaa aaagaaagaa gaaaggaact gaacacggga ctaatttcct
    13981 aggaaaaaaa taaaactacc tggacgcaag cgtcatcgtt agttttctgc gtcgtaggcg
    14041 tctgatgcta aagcacctag tcgccctgac gtgggttcgt cttcgaaatc aaagatttcg
    14101 gacttaccgc atatttttct acggaaagtt tcgacaagtc gaaacgcccc tttatatctt
    14161 aattatgaaa actccaatta tttctattat catgggctcc aaatctgact gggcaaccat
    14221 gcaaaaaacg gctgaagttc tagacaactt tggcattgct tacgagaaga aagtcgtctc
    14281 tgctcaccgc acgcccgacc tcatgttcaa gcatgctgag gaagcacgtg gtcgtggtat
    14341 caaaatcatc atcgcaggag caggtggggc agctcacttg cctggtatgg tggcagctaa
    14401 aacaacgctg cctgttatcg gtgtgccagt caaatcacgt gtcctttcgg gtttggattc
    14461 gctctattcc attgtgcaga tgcctggcgg tgtgcctgtg gcaaccatgg ctatcggaga
    14521 agcgggagcg actaacgctg ccctgacagc cctccgtatc ctctctatcg aggaccaaaa
    14581 tctggctgat gcgctagctc atttccatga ggaacaagga aaaatcgcag aggagtcaag
    14641 tggtgaactc atttaagact attgggatta ttggtggtgg tcagctaggg cagatgatgg
    14701 ccattgcggc catctatatg ggccacaagg tcatcacgct ggatcctact aacgactgcc
    14761 ctgcctcccg cgttagcgag gtaattgtgg cgccttacga tgatgttgag gctttgggac
    14821 aattggctgc gcgttgcgat gtgttgactt atgagtttga gaatgttgat gcggacggtt
    14881 tggatgccgt tgtgtcagct tgtcagctac cgcaggggac cgatctgctc cgcatttccc
    14941 aaaaccgtat ctttgaaaaa gactttctgg caaataaggc tggtgtgact attgctccct
    15001 ataaggtggt gacgtctagc cttgacctag aggggcttga ttttaccaag acctatgtcc
    15061 tcaagacagc gacaggtggt tatgacggtc atgggcaaaa ggttatccgc tcagcagaag
    15121 atttgccaga ggcgcagcaa ttagctaact cagctcagtg tgtcttggaa gagtttgtca
    15181 actttgacct tgaaatctca gtcatcgtgt ctggaaatgg caaggatgtg acggtctttc
    15241 ccgttcagga aaatatccac cgcaacaata tcctatcaaa aaccatcgta ccagctcgca
    15301 tctcagatca actggctgat aaggctaaga aaacggcggt gcagattgcc aagaaactcc
    15361 agctgtcagg aactctctgt gtggaaatgt ttacgaccgc agatgatatc attgtcaatg
    15421 aaattgcccc acgcccccac aactcagggc actattctat cgaagcctgc gacttttcac
    15481 agtttgacac ccacatcttg ggtgtgttgg gcgcaccact tcctcagatt cagctccacg
    15541 ccccagccgt catgctcaat gtcctaggac aacatgtcca gcaggcaact gactatgttg
    15601 ccaaaaaccc tagcgcccac ctccacatgt atggtaaact agaagcgaaa cataaccgca
    15661 aaatgggaca tgtgacggtg tttgcaaaag atgcggatga ggtgaaggag ttttaaagag
    15721 ttaattagga gatgtttatt ttatggcaaa tattcaaata aatagaataa aaagtaaatt
    15781 gacagaactg tttagtagta ttatctatat gggtaatatc aaagttgatg aggactctga
    15841 agagtataaa aaaatgtggt atagtagggc ttactcagct tattcaactt ttctacttgg
    15901 agcagaaaat gtagatgaag caactaagtc agttacggat ggatttgctg ataatggcat
    15961 agatgccatc tataacgata aaaataaaaa aattttatat tttattcaaa ctaaatttag
    16021 caatgaagca aacggaagca tttctgaagg tgatacatta aaatttatta aaggggttaa
    16081 aaaaatcacc acttcagatt tttcaaattt taatgatctc atagtagcaa agcaaaaaga
    16141 aatagaaaaa gctttatcag attttaatta tcgaatagaa attatagttt gtacttcctc
    16201 taatcaaaat atatcttcaa atagtcaaga agtaatagat gattttttgc agggtgtaaa
    16261 ttctgatggt agtgatgatg tagtaacata cagacatata aaacttaatg atgtttatga
    16321 tcatatggct aatcaaggag tggctcctaa aatagatttc gaggacgttg tcgttcataa
    16381 ttggggcaga cctacattat acaatgacag ttataaagtc tattatggat ggttatctgc
    16441 tactgatata gttaactgga aagagcaata tggagatcga atatttgaaa agaatattcg
    16501 gaactttaag caaaatacgg atgttaataa cggaatactg aaggttttaa gagaagaacc
    16561 tgaaaatttt tatttgtata ataacggaat aaaaataatt tctgaaaaag ttgaaaaaaa
    16621 tctaaaaggt gtttcaacaa cggattatat caatttaaaa ttgattggag caagtataat
    16681 taatggagcg caaacaacgg gttctttatt tgaagcatac cattctgaag atattgattt
    16741 gacaaatgtt tcagtccaag ttcaaataat agtattagga gaagatattg ataatatcgg
    16801 acctaaaata actaagttat cgaatactca aaatagaata gaaaataaag attttgctgc
    16861 tcaggataaa gaacaagaaa gattgatgaa agatcttgca attgatggta aacagtatgt
    16921 gtatcgtcaa ggtgtcgagc tacctaattc tgatgaaggt tgtgatctgg atagtgcaac
    16981 agtggctcta ggttgctatt tagatgatgt tgcaatttct acacagatga agagagcata
    17041 tggtagcatt tttgacaata ctaataaacc tccatataag ctaatcttta attccggaac
    17101 ttctgcatat aaattatgga attgcgttga agtgtatcgc gaattacaaa acatagaagg
    17161 ggagtatcaa caagatccaa ataatcaatc taaaaagttg atgtctattc atggaaatag
    17221 atttattctt catttgattt tccaaaagtt aaaaaataac ccaagtttag atttcgagaa
    17281 tggatatatt acaacttttc caaatttaac tgaagagtat tgtttaataa tggaagaatt
    17341 gataagagcg aaggaagagt tatacccaga agcttaacca gctaatatat ttaaaaatgg
    17401 taaacgtact aaagaattaa aagaaaaaat gtctttttaa taagataagg ggaaaaaatg
    17461 ctagaacgtt attcacgccc tgagatggcg gcaatttgga cagaggaaaa taaataccgt
    17521 gcttggttgg aggttgagat tctggctgac gaggcatggg cagagttggg tgagattcct
    17581 aaggaggatg tggctaagat tcgtgaaaaa gcggattttg acattgaccg catttttgag
    17641 attgagcaga acacacgtca cgatgtggtg gctttcacgc gtgcggtttc tgagacgctt
    17701 ggtgaggagc gcaagtgggt gcactacggt ttgacctcga ctgacgtggt ggacactgcc
    17761 tacggttacc tctacaagca agctaacgac attatccgtc gcgatcttga gaatttcacc
    17821 aatatcgtgg cagacaaggc gcgtgagcac aaaatgacca tcatgatggg tcgtacccac
    17881 ggtgttcacg ccgagccaac gactttcggt cttaagttgg cgacttggta cagcgagatg
    17941 aaacgtaata ttgagcgttt tgaacatgct gccgcaggtg tggaagctgg taagatttca
    18001 ggtgccgttg gtaactttgc caacatccca ccttttgtgg aagaatatgt ctgtgacaaa
    18061 ttaggcattc gtccgcaaga aatttcaaca caagttcttc cacgtgacct tcacgcagaa
    18121 tactttgcag tgcttgcaag tattgcaact tctatcgaac gtatggcgac agagattcga
    18181 ggtctgcaaa agtcagaaca acgtgaagtt gaagaattct ttgccaaagg tcagaaaggt
    18241 agctctgcta tgcctcacaa acgcaaccca atcggttcag agaacatgac agggctagcg
    18301 cgcgtgattc gtggtcacat ggtgacagct tatgagaacg tgtcactttg gcatgagcgt
    18361 gatatttcac actcatcagc tgagcgtatc atcacacctg acacaactat cttgattgac
    18421 tacatgctca accgctttgg caatatcgtt aagaacttga ctgtcttccc ggaaaatatg
    18481 atgcgcaata tggaatcaac ttttggttta atttatagtc aacgtgttat gctcaaattg
    18541 attgaaaaag gaatgacacg agaagaagct tatgacttag ttcaacctaa gacagcttat
    18601 tcctgggaca atcaagtgga tttcaaacca cttttagaag aagacaccaa agttacctct
    18661 tgtcttacac aagaagaaat tgatgaacta tttaatccga tttattacac aaaacgtgtt
    18721 gatgatattt ttaagcgttt agggatttaa tataaaaaaa agaaggtgat tcccttcttt
    18781 tttatttaaa aaacgaatat tttctttaaa aatattaaaa aagttagttt ttaattagac
    18841 gctgtgatat aataaagaaa acagttgttg gagagaaacc atgttagaac attttggtgg
    18901 aaaagtaaaa gtgttaagac ttgaaaagag gattagtcgc gaggacttgt gtggggatga
    18961 gtctgaactt tctgttcgtc aattagcacg gatagaacta ggtcaatcca taccaagttt
    19021 aagtaaggtt atttttattg caaaagcctt aaacgttagt gtcggttact taactgatgg
    19081 tgctgattta gaactaccta agcgttacaa agaattaaaa taccttatct taaggacacc
    19141 aacttacatg gatgatggaa aattacaagt acgagaagag cagtttgatg aaatttttga
    19201 ggattattat gataaattac cagaggaaga gaaaataatc attgattgtt tacaggcaac
    19261 tttagatact ttattgagtg agaatactaa ctttggcatt gacttacttc aagaatattt
    19321 taatcaaata aagactaagg tacgctttag gcaaaatgat ttaatacttc tagaattata
    19381 tttagcttat cttgatattg agggaatgga tggacaatat tcagataaga ttttttatga
    19441 ttctttatta gataatctat cagaacaatt tgaattagat gaattattta tagttaataa
    19501 gattatcatt gatatttcat cactaagctt aaaaaataac cgacttgata atttagaaaa
    19561 agcgattgaa atgagtcaaa aaattatggc gaaaattcaa gattggaata gaatgcctat
    19621 tttaaaacta atagaatgga aatatttttt aatcaaacaa aaagatataa taaaagcaga
    19681 acaatcattt atgaaggcat gtttgttcgc ccaaatgaca gctgatcagt atttggaaaa
    19741 caagttaatt caagagtggg aaaaagatgt taaaagtt
//
LOCUS       DAUCWU010000024        18914 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUCWU010000024 DAUCWU010000000
VERSION     DAUCWU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.024"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            140..1579
                     /locus_tag="VGY24_000809"
     CDS             140..1579
                     /locus_tag="VGY24_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ4283808.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            1584..2549
                     /locus_tag="VGY24_000810"
     CDS             1584..2549
                     /locus_tag="VGY24_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4283809.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(2690..3142)
                     /gene="nusB"
                     /locus_tag="VGY24_000811"
     CDS             complement(2690..3142)
                     /gene="nusB"
                     /locus_tag="VGY24_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ4283810.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTR
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(3135..3524)
                     /locus_tag="VGY24_000812"
     CDS             complement(3135..3524)
                     /locus_tag="VGY24_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4283811.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            complement(3570..4127)
                     /gene="efp"
                     /locus_tag="VGY24_000813"
     CDS             complement(3570..4127)
                     /gene="efp"
                     /locus_tag="VGY24_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ4283812.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            complement(4223..4684)
                     /locus_tag="VGY24_000814"
     CDS             complement(4223..4684)
                     /locus_tag="VGY24_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ4283813.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            complement(4719..5792)
                     /locus_tag="VGY24_000815"
     CDS             complement(4719..5792)
                     /locus_tag="VGY24_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ4283814.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDASEIETIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSRFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLASKELIVL"
     gene            complement(5907..8735)
                     /gene="uvrA"
                     /locus_tag="VGY24_000816"
     CDS             complement(5907..8735)
                     /gene="uvrA"
                     /locus_tag="VGY24_000816"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ4283815.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEITASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            8938..9882
                     /locus_tag="VGY24_000817"
     CDS             8938..9882
                     /locus_tag="VGY24_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ4283816.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            10014..10670
                     /locus_tag="VGY24_000818"
     CDS             10014..10670
                     /locus_tag="VGY24_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ4283817.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(10803..11042)
                     /locus_tag="VGY24_000819"
     CDS             complement(10803..11042)
                     /locus_tag="VGY24_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ4283818.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(11207..11698)
                     /locus_tag="VGY24_000820"
     CDS             complement(11207..11698)
                     /locus_tag="VGY24_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ4283819.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            complement(11720..12010)
                     /locus_tag="VGY24_000821"
     CDS             complement(11720..12010)
                     /locus_tag="VGY24_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ4283820.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            complement(12183..12476)
                     /locus_tag="VGY24_000822"
     CDS             complement(12183..12476)
                     /locus_tag="VGY24_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283821.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            12674..13798
                     /gene="mutY"
                     /locus_tag="VGY24_000823"
     CDS             12674..13798
                     /gene="mutY"
                     /locus_tag="VGY24_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ4283822.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            13975..14562
                     /locus_tag="VGY24_000824"
                     /pseudo
     CDS             13975..14562
                     /locus_tag="VGY24_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(14614..14928)
                     /gene="trxA"
                     /locus_tag="VGY24_000825"
     CDS             complement(14614..14928)
                     /gene="trxA"
                     /locus_tag="VGY24_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ4283823.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(15009..15509)
                     /locus_tag="VGY24_000826"
     CDS             complement(15009..15509)
                     /locus_tag="VGY24_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ4283824.1"
                     /translation="MESYEHFYAKLSQPFRKTPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            complement(15513..17852)
                     /locus_tag="VGY24_000827"
     CDS             complement(15513..17852)
                     /locus_tag="VGY24_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ4283825.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQLPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHTFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKITVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(18001..18546)
                     /locus_tag="VGY24_000828"
     CDS             complement(18001..18546)
                     /locus_tag="VGY24_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ4283826.1"
                     /translation="MLSLLIVIVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            complement(18549..18857)
                     /locus_tag="VGY24_000829"
     CDS             complement(18549..18857)
                     /locus_tag="VGY24_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283827.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
BASE COUNT         5864 a         3979 c         3312 g         5759 t
ORIGIN      
        1 cttgactatc attatatcac taatattttt tatgtcaagc gtttaacata tttttatttt
       61 atatgcttta aattcttgat ttttaaacag aaaacgttta gtatattctt gaagacgact
      121 ataaaacaag gagattttga tggatctacc acaagctatt cgctaccgcc cttacaaaga
      181 gtggagctca aaagactacc aagctattac tgaaaaaatg gcccaatccc catggcacag
      241 ccaatttcat gtcgaaccta agacaggatt actcaatgac cccaatgggt tttcttattt
      301 caacggtcgc taccatcttt tttatcaaaa ctggccttat ggggctgcgc atggtctgaa
      361 acaatgggta cacatgacat ccacagacct cgtccatttt acggaaacaa ggagccgcct
      421 tctgccagac cacgctcacg atagtcatgg ggcttactca ggttctgcct atgctattga
      481 tgacaagctc ttcctctttt acactggtaa tgttcgcgat gccaattggg tcagaacacc
      541 attacaggtt ggcgcatgga tggataaaca aggaaatatt tccaaaatcc cgcaggtttt
      601 gattgaacag cccgatgatg ttacagaaca ttttcgggat cctcagcttt tttcttatca
      661 aggtcaattc tatgctatta ttggcgccca aggtttagat ggcaaaggaa aaatcaaact
      721 ttacaaagct gtggacaatc atgtggacaa ctggcgattt atagccgatt tggactttga
      781 cgattctggt acggaataca tgattgaatg ccccaacctt gtgtttgtcg atgacaaacc
      841 tgttttgatt ttcagcccac aaggactggc taaagctgat ttagattatc aaaatatcta
      901 ccccaatacc tataaaattt ttgaaagctt caatccagaa actggccaat tgctgggtgg
      961 aggagccctt caaaatcttg actttggttt tgaagcttat gccactcaag cctttagtag
     1021 tccagatggc agggttctag ccgtgtcttg gattgggtta ccagatattg attatccgac
     1081 agatcgttat gactatcaag gagctcttag tttagtgaaa gaattaagga ttaaagacgg
     1141 aattctctat caaacacctg tttcagccct acaaaatctt cgagggccag cagaattatt
     1201 tcataataaa atagacagtt ctaactgtta tgaattggaa ttgacgattc caggccaaaa
     1261 aaagcttgac ctgctcctat ttgccgacca aaaagggaat ggcctaagat tgaaagtgga
     1321 tacaacaaaa ggacagttaa gcattgaccg aagtcgggca ggggttcaat atgcccaaga
     1381 ctatggtacg gtgcgctctt gccaaatccc acaaggccat gttacactta atgtctatgt
     1441 ggacaattcc attcttgaaa tctttattaa ccaagggcaa aaagtcctaa cgagtcgtgt
     1501 tttcccaacc caaggacaaa caggtatcca agtagtagaa ggacaagctt ttggacatta
     1561 ttatgaaatg aggtattagg caagtggtcg caaaattaac agacgtggca gccttggcgg
     1621 gtgtcagtcc aacaactgtt tcgcgagtaa tcaacaaaaa aggctacctt tcccaaaaaa
     1681 cagtgaataa ggtgaataag gccatgcgtg aattgggcta caagcctaac aatctcgcta
     1741 gaagtttgca aggtaaatca acccaactca ttgggcttat cttccccaac attagcaata
     1801 tcttttacgc tgaactaatt gaacatttgg aaattgaatt gtttaaacag ggctacaaaa
     1861 ctattatttg caatagtgaa cataatcccg tcaaagaacg cgaatactta gaaatgcttg
     1921 cggccaatca agtggacggc attatttctt ccagtcataa tttgggaatt gaggattatg
     1981 agcgtgtcga agctcctatt gtagcttttg accgtaatct ggcgccaaat atcccggtta
     2041 tttcttcaga taattttgaa ggtggtaaat tagccgcaca aactttgcaa aaacacggtt
     2101 gtcaaaacat tgtcatgatt acaggtaatg acaattctga ttcgcccaca gggttgcgcc
     2161 agctagggtt taactaccag ctcaaacgaa gtgcagaaat catcaaatta ccaaataacc
     2221 tatctcctgt tagacgcgaa atggaaatta aatccatttt ggcaactcga aaacctgatg
     2281 gccttttcgt ttctgatgat ttaactgcta tcctcattat gaaagtagcc aaacaacttc
     2341 acattactat tccagaggat atgaaagtca taggttatga tggaacgaca tttatccagc
     2401 aatatgttcc tcaattagcg accattcgtc aacctattga tgagattgct aaattgagtg
     2461 tcgaaatact cattaaaaaa attaaaaaag aaaaaaccag taaagactat attttaccga
     2521 ttacgctact tccaggagca agtatttaaa caaagcataa ccaatcacta attactctaa
     2581 aagacaaaca agcatactct ttctagatgc gagagtatgc ttgtttgtct tttaatattc
     2641 gtacataaat tgaaaaaatc ggatacaatc ctaagattgc tccgacttat tatgatttat
     2701 ttgctgaagg agcttccgag acatattggc taagtaagcc attaataaat ttagcagagg
     2761 tttcatcaga atatttttta gctacttcaa tgatttcgtt tagtgcaaca cggtctggtg
     2821 tttcgtcaaa atacttgatt tcaaaaagac ctagtcgtaa taacgttttg tctgttagtg
     2881 tcaagcgttc taacgaccaa ccttttttca aatgagttga tatcaagtta tccaactctt
     2941 ctttatgatt attgacaccc gtcacaagac ttaataaaaa aatcggcagc tccaatactt
     3001 gagcgtcctc ccttgttact ttatcataac cataggcaaa ctgagaagct gccaataact
     3061 cagctcccat ctctatgtta aataaagcct gaaaggcacg ttcacgcaaa tctcttcttg
     3121 aattttgaaa gctgttagtc atccaagaaa tcctcatcaa aaagtgactt caaatctggt
     3181 tttggtgtct tttccgcaac aataccttca acatggatat taactgcaga aataggcact
     3241 tctgccatat cgtacacagc agatttgact gttttttgaa tattcataga tacagtcggg
     3301 accttgactc catattgcaa gtaaacataa atatctgctg taacacttcc atcttcttct
     3361 gtctggagat aaaccccttt accaagactt gctttgttaa agctatctgc cattttcttg
     3421 ttatggagtg agtggacgcc ctcaacttgt gtcgtagcaa tccccgtaat aacctcaagc
     3481 actcgtggtg aaatgacgat ttcaccgata tattcagttg tcatagcatt tcctttctct
     3541 tttttagact aatctgtttt gacaaaggat taagcacgag aaacgtaagt accttctgca
     3601 gtgttaatga ttagtttttg gccagcttcg ataaagtctg gaacgttaac gacaagacct
     3661 gtctcaagag ttgcaggttt ccctgaaccc gtcactgtcg ctcctttaat agatggttgt
     3721 gtttccgcaa cggtcaattc aacagttgtt ggaaccgtta ccccaatcac ttcacttcca
     3781 taaaattgga ttttcacgtc tgagttttca agaatgtaaa gcaattcttg ctcaacgtta
     3841 gcaactggaa tttcgtactg atcataagtg tcagtgttca tgaagtaagc agtgtcatcc
     3901 attttgtata ggtattgtgc tgggacagtt tcaatgatgg cttgctcaaa tttttcatct
     3961 gggcggtaag ttgtgtcaaa agtagaacct gtacgcacat cacgtagttt catacgcatg
     4021 atagtgtttc ctttacctgg tttgtggtgg ctagcttcaa ggacacggat taattttcct
     4081 tctgcttcaa atgtcatacc tgctttaagc ttacttgctt caatcattat tattacctct
     4141 tactaaaata tttatcactt cattttacca caaaagtggc cttggctcaa atcttttatg
     4201 accgtccttt taggtacact ttctattgtt ccttatcgcc tcctaaaaca atttgaggca
     4261 catcatgttg cacataggca accccttttt tctccatcag ttcaatggca aaggggtgcg
     4321 gacggtaatg tgctttatag gtaatctttg taatccccgc ctgcaaaagc gccttagtgc
     4381 agttaataca aggaaaatga gtcacgtaaa tttcagttcc gtccgttgaa attccttctt
     4441 tcgcacattg aatcaaagca ttcatttcag catgaacagt gcgaatacaa tgtccgtctt
     4501 ccatataatg ccctgcttca ttgcagttat ccgtagcaga aactccgcca ttgtaacctg
     4561 tggcaataat gcggttatcc tttaccaaaa cagctcccac aaaggcgcga tcacaggtag
     4621 agcgcttgga aatcagctct gcattggcca taaaatagtc ttgccatgat aaacgatttg
     4681 tcattgcttc tcccctaatc ctttcctgaa ccattctttt acaatacaat taattctttg
     4741 gatgccaagg tcaagacttg acaaccagtt tttgtgataa ccaagtcgtc ttcgatacgg
     4801 acaccatatt tgttatccaa atagatacct ggctcatctg ttaccaccat tccagcttgg
     4861 agaagttgct cagatttccc aaaaaatgga ttctcatgga tgtcaagccc gataccatga
     4921 ccaatgccat gtgtgaagcg actaccataa cccgcctcag tgatgagttg gcgcggaata
     4981 ccgtcaaagt cactataagt catgccagcg ctagctttag caatgagagc cttattagca
     5041 gcaagaacaa gagcataaat ctcacgttct tcatcagtaa cttggccaat atgaatggtc
     5101 ctcgtcatat cactaacata gtgattgtag taacacccaa agtccatggt caagctctct
     5161 ttattctgga taaccttgtc actggcgcgt ccatgaggca tggcagagcg atagcctgaa
     5221 gctacaatga tatcaaatga tgtgccgctg gcaccatact gacgcatgcg aaaatctaaa
     5281 aaattagcca ggtcacgttc agtggttgtc cctggtttaa taaaatcaag agcatcttca
     5341 aatgctttat ctgagatcga gcacgcttta gcaatggtct cgatttcaga ggcgtcctta
     5401 ataagacgta aatgctccac aaaacctgac tgagcaagca aggttattcc tgacagttct
     5461 gcttgcatgg cctgataaaa agaaaacgat acctggtcct caaaaccaag gcaatctatt
     5521 tgatcagcct ctaacaattc tgccacaacc ttaagcggcg tgcggctttc gataatatca
     5581 aatccctcaa cactagcttt agcaagcaag gtataacgtg aatctgtgat caaaacacga
     5641 cgtttggccg ttatcaaaac agttgctgca gttccagaaa aacctgtcaa gtaataacta
     5701 ttggttaaat gggtgaccag aagagcctct agccccttct ctgccatctg ccttaggcag
     5761 tgacctaatc gttgctctaa aaatcctgac atcgcaaact cctttggtaa cttttgagaa
     5821 tattgtagca tgaaaaaact actttaagtt taccatgatt tgagagcatt taaaagatta
     5881 aaaggagctc aaacgaactc ctaatcctat tgttgtagtt ttactttcaa ataatgacca
     5941 gtatagcttt ctttaacttg agctacctct tctggtgtcc ccgtggcaac aatctgacca
     6001 cctccgacac caccttctgg tcctaagtca ataatatggt cagcagattt gataacgtcc
     6061 aaattatgct caatcacaag aaccgtatta ccatcgtcta caaagcgttc caaaactttt
     6121 aatagccttg caatatcatc tgtgtgtaaa ccagtcgttg gctcatctag gatatacaag
     6181 ctcttacctg tcgagcgttt gtgcaattca ctagctaatt tcatccgctg cgcttctcct
     6241 ccagatagag tagtagctgg ttgacccaag gtcacataac ccaaaccaac atccttaatg
     6301 gtttgaattt ttcgggcaat tttagggata gcagagaaaa ataccaaggc atcatcgaca
     6361 gtcatgtcaa gcacttcagc aatatttttt tctttataat gcacctcaag cgtctcagaa
     6421 ttataccgtc tgccgtggca aacctcacaa ggcacataaa catctggcaa aaagtgcatt
     6481 tcgattttga taatcccatc acctgaacaa gcctcacaac gcccaccttt gacattaaaa
     6541 gagaaacggc cttttttgta accacgaatc ttagcttcgt tggtttgggc aaacaagtct
     6601 cgaatatcat caaagacacc cgtataggtg gctggatttg accgtggcgt ccttccaatc
     6661 ggactttggt caatgtcaat caagcgttca atatgttcaa taccagaaat agagtgatat
     6721 ttaccaggct tatcagcatt acggtttaat ttttgggcga cagccttttt caaaatacta
     6781 ttaaccaagg ttgatttccc tgagcctgat acccctgtga ccgcaataaa tttacccagt
     6841 gggaatctca cgtcgaggct ttgaagatta ttctgcgctg ctccttttat ttcaatgaat
     6901 cgcccattac cagaacgacg ctctaatggc actggaatga attttttacc agacaaatat
     6961 tgacccgtga tggatttttt attcttagcc acttgtttcg gcgttccaga ggcagtaatt
     7021 tctccaccga actcacctgc ccccggtccc acatcaatca gccagtctgc ctgcatcatg
     7081 gtgtcttcat cgtgttctac cacaattaag gtattaccca aatcacgcat ttttttcagg
     7141 ctttcgatca aacggtcatt gtctctttgg tgcaatccaa tagaaggctc atccaagata
     7201 tagagcaccc ctgataagtt agaaccaatt tgtgtcgcta acctgattcg ttgactttcc
     7261 ccacctgata aagtcccagc agcacgtgat aaggtcagat aattcagtcc cacattattt
     7321 aagaaggtca ggcggtcatg aatttctttg acaatcggtt tagcaatggt actttcattt
     7381 tctgtgagtt ccagttcttc caacaactgc aagtggtcgg caatcgataa ttctgaaatt
     7441 tgaccaatat gaggtccttc ttcgccaccc acatgcacac acaaggcttg gtcattcaaa
     7501 cggtaaccat gacaagtcgc acaggtcaat tcgttcatat aaccacgcat cacattgcgc
     7561 gtataatcac tattagtttc gtgataacgg cgattaacat tagttacaac accttcaaaa
     7621 ggaatgtcaa tgttacgctc accaccaaaa tcattcacat agtgaaaatg aaattctcta
     7681 tcacctgaac cataaagcac taaatctctt tcttcctcag tcaaggcctc aaaaggtgta
     7741 tccatatcca ccccaaaact tgccatagct tgctcaagca tggttgggta ataattggag
     7801 gaaataggat tccaaggcgc caaagctccc tctctcaaag acttacttgg gtcaggaaca
     7861 accaagtcca aatccacttc caacttaatg cctagcccat cacaggtcgg acaagagccg
     7921 aacggcgcat taaaagagaa gagtcttggt tctaattcag gaaccgtaaa tccacagaca
     7981 ggacaggaat aatgctcaga aaagagcagt tcattgccat ccatagtgtc aatcataagg
     8041 tagccatctc ccagccttaa agctgcttca accgaatcaa ataaacggct acgaatacca
     8101 tccttattaa ccagacgatc aataaccacc tcgatgttgt gcatcttact ttttgacagc
     8161 tccggcactt ctgtcacgtc aaaaatatcc ccgtcaaccc gcacacggac ataaccatct
     8221 ttttgaattt tctcaaaaat ggttttgtgt tgaccttttt tccggcgaac aataggcgct
     8281 aaaatctgca tgcgtgtccg ttcagggagg gctaagacct gctctacaat ttgttctgca
     8341 gaagatgctg taatggcccc atgcccatta atacagtaag gtgtgcccac acgcgcatat
     8401 agtaaacgta aataatcatt gatttcagtc accgtaccaa ccgttgagcg gggattctta
     8461 ctggtagttt tttgatcaat ggaaatagca gggctaagac cgtcaatgga atccacatct
     8521 ggtttttcca tattacccaa gaactgtcta gcataagctg ataaactttc tacgtaacga
     8581 cgttgccctt ccgcataaat agtatcaaag gctagactag attttcctga ccctgataac
     8641 cctgtcacaa caactaattt gtctcgtgga atttccacgt caatattttt taaattgtgg
     8701 gctcttgccc catgaattat tattttattt tgcatcttga atgtgcctaa aggcaccctc
     8761 ctcatcgagc tttcttatat tataacaaaa atttctgata acctattgcc ccaaactttg
     8821 cgaattttgg tataatagat aagtattgag aatttttcag aaaatccttg agaaggcttt
     8881 attatcaagg tttgatgtca atctctacca gtcaatcatc aaaaaggagg gcttgtcatg
     8941 aaacaaatgt ttctttcatc ggcaattgaa ttcaaagaaa tagaaacatt cgagccagga
     9001 gcttggatta aactggttaa cccttcccaa gaagagtcta tgaagatcgc cgatcaattt
     9061 aacattgata tttctgactt gcgcgcccct ttggacgtgg aagaaacctc acgtattgcc
     9121 gtagaagatg attataccct aattatcgta gacgttccga tatacgaaga acgtaacaat
     9181 aaaagttact atattactat gcctttaggc attattgtaa cggaaaatgc tgttatcaca
     9241 acttgtctgc atgacatgac ccttttcgat cattttcaca accgccgggt caaaaatttc
     9301 tataccttca tgaagacacg ttttgttttt cagattctat atcgcaacgc cgagctcttt
     9361 ctgactgcct tgaggaccat tgatcgccaa agcgaacgtt tggaagctca gcttgaagca
     9421 gctactcgaa atgaagaact tatcgacatg atggaacttg aaaaatccat tgtctactta
     9481 aaggcttcct tgaaattcaa cgaacgcatc gtcaagaaat tatccagctc aacaagctct
     9541 ctcaagaaat acattgagga cgaggatttg ctagaagata ccttgattga aacccaacag
     9601 gccattgaga tggctggcat ttatgaaaat gtcttgaatg ccatgactga aaccactgct
     9661 tctattatca acaacaacca gaacactatt atgaaaacac tggccttgat gaccatggct
     9721 ttagatatac caactgtgat tttctccgcc tatggtatga atttccaaaa caactggtta
     9781 ccactaaatg gcctagaaca tgctttttgg tatattacct tgattgctat gctacttagt
     9841 tcctttgttg tgatttactt tattagaaaa aaatggttct agagtggagc aagctgcaga
     9901 atgcggttaa gacctgacaa aactctgtgt caaacggcta gctccaaagt ttcattacaa
     9961 ctagctaata ccattgactt actttaacta acccatataa agaagggatt cccatggaat
    10021 tacaagaact taccaaaaaa aatcaagaat ttatccacac cgctacgaac aaattaatcc
    10081 aagacggcaa gtctgatgag gatattaagc tgattctcga agaagccatt ccagctattc
    10141 tagaaaacca gaaaaaaggt gtcaccgctc gaaatctatt aggcactccg actgcttggg
    10201 cagcttcttt tagccaagac cctagtcaaa gagcagcaga aactgacaaa aacaccaatc
    10261 cttggttgat gtggctagat acctctttgc tattcatcgg aattgttgcc cttttaaatg
    10321 gcatcatgac cttcttcaac acaaacgcta cagttacagg attgatttcc ttactggcac
    10381 ttggttttgg tggaggagca tccatgtacg ctacttatta ctttatttac cgtcatctcg
    10441 gaaaagataa gagccttcgt ccaagttggt tcaaaatcat cgcagcctta tcattagcca
    10501 tgttgatttg gattgccttg tactctgcaa ctgccttttt accaacctct cttaacccac
    10561 agttgcctcc tcttgccttg ctcattattg gtggtgtttc ccttgcttta cgctattacc
    10621 tacaacgcaa atacaacatt caaaacacta tgtcacctgt taacaaataa caatcatttc
    10681 atccttgctg tctacgcaag gattttcgtt tactatcatt ctctctttta gaaaaaactc
    10741 acctatacgc ctagactagt cctagtcaac taaaaaacag tccgaaatgg actgtttgtt
    10801 tattagtctt cgtttacgta aggcataaga gccataacgc gagcacgttt gattgctgtt
    10861 gttactttac gttggttttt agctgaagtt cctgttacac gacgaggaag aatttttcca
    10921 cgttctgaaa cgaaacggct aagaagctca gtatctttgt aatcaacata ttcaattttg
    10981 ttagctgcga tgaagtcaac ttttttacgg cgtttgaatc cgccacgacg ttgttgagcc
    11041 atgtattttc tcctttataa attatgatgt taagggctct taaaaaacga ctgaatttta
    11101 gaaaactggc aagaaatctt gatttcctag ggagttgatc taaattctaa gttctaaatt
    11161 tttagcccga actcaaataa tttcgtattc gttcaatcca ttctgactag aatggaagat
    11221 cgtcatctga gatatccatc gggtttgagt tcccaaatgg gctatcatct cttccaaagt
    11281 taggcgtttg ttgtgcaggc gctgaataac tgtttgatga tgaagtgtta ttgttaaaac
    11341 caccattaaa tgagccagtt gagccacctt cacgtgtagc acgactttcc aacatttgga
    11401 aattatctgc aacaacttct gttacataga cacgttgtcc ttgttggttt tcgtagttac
    11461 gtgtctgaat acgacccgta actccgatca aggcaccttt tttagcccag ttcgctaaat
    11521 tttcagccgg ttgacgccag atcacacagt taatgaaatc tgcctcgcgt tcaccatttt
    11581 ggcttttaaa ggtacggtta acagcaagtg tgaaggtagc cacagctact tgacttggtg
    11641 tgtaacgaag ttctgcatcc ttggtcatac gaccaactag tactacatta ttaatcataa
    11701 actaccctct tagttggaat taagcgtcaa gtttaacgat catgtgacga agaatgtcac
    11761 cattgatttt tgaaagacgg tcaaactcgt taagagctgc tgcgtcagtc gcttcaaggt
    11821 taacaatgtg gtaaagtcct tcacggaaat cgttgatttc gtatgcaaga cgacgttttt
    11881 cccaatcttt tgattcaaca acagttgcac cgttgtcagt caagatagag tcaaagcgtg
    11941 ctaccaaagc gtttttagct tcttcttcaa tgtttggacg aatgatataa agaatttcgt
    12001 atttagccat tgatattttc ctccttttgg tctaatgact cactactttt ggagtgagta
    12061 agtgaggtat actcacagaa tcttattgta cactaatagt caatcaaaag caaggaactt
    12121 tttctacttg actaagtttt gtcaaacatt ttctttcacg agttttaaaa attggcactt
    12181 agtcagactc ctaaaaaata ggtgaaaaaa gaaaaagcaa ggaaaagttt taaccaaagt
    12241 gcaaaatata aaattagggc caaaacaaaa aggttaaact ttttaaaaat aatagcaacc
    12301 atggcacata cgatacctaa tccaggaaaa acttgcgtca tgatcagcca ttccaactgt
    12361 agtccgctca aagaaggatc aaatggataa atatagttaa gaaataacac aagaatcatc
    12421 attataaaaa cgaaccacga ataccgatca ataagattct ttaatttctg gaacatgtcc
    12481 cctccaaatt aaaacctgtc tgcttctatc gttaatttca gttattctaa atcaacattt
    12541 agatgaggta aacctaatat tattataata tttccttttc tttttcgcaa atacctaaaa
    12601 tcttttttta ctctttttaa ggctcctgtg ttacaataac aacatgattg aattaaaaga
    12661 ttatggtatt aatatgtggg ataatgagac aatcgcttct tttcgtcgca cacttttagg
    12721 gtggtatgac caagaaaagc gtgatctccc ttggcgaaga actactaatc catattatat
    12781 ttgggtttct gaaatcatgt tgcaacaaac acaagttaat acggtaatcc cttattataa
    12841 acgttttcta gagtggtttc ctcaaataaa agatttggca gacgctcctg aagaacaatt
    12901 actaaaggca tgggagggac taggctatta ttctcgggtg cgtaatatgc aaaaggctgc
    12961 acagcaagtg atggttgatt ttggtggcat atttcctcac acttatgatg atattgcttc
    13021 attaaaaggg attggtcctt atactgcagg tgctatcgcc agcatctcat tcaacttacc
    13081 agagcctgct gttgatggca atgtgatgcg tgtcatggct cgtttgtttg aagttaatta
    13141 tgacatcggt gaccctaaaa atcgtaaaat ttttcaagct attatggaaa tactaattga
    13201 ccctgacaga cccggtgatt ttaaccaagc acttatggat ctaggtacag acatcgaatc
    13261 cgctaagaca cctaggcctg atgaaagccc tattcgtttc tttaacgctg cttatttgaa
    13321 tggtacttat ggtaaatatc ccataaaaaa tccaaagaaa aagcctaaac caatgcgaat
    13381 tcaggctttt gtcattcgta accaaaatgg ccaatatcta ctggagaaaa atactaaagg
    13441 gagacttcta ggaggttttt ggtcatttcc tattatcgaa acgtcacctc tttctcaaca
    13501 attagattta tttgatgaca atcaatccaa ccccataatc tggcaaaccc aaaatgaaac
    13561 tttcgaaaga gaatatcaat taaagccaca atggactgat aatcactttc caaatattaa
    13621 gcacactttt agtcaccaga agtggacaat agaactcata gagggggtgg tcaaagcaac
    13681 agatttacct aatgctcctc acctaaagtg ggtagcaatt gaggactttt ctctgtatcc
    13741 ctttgcaaca ccacaaaaga aaatgctaga gacctactta aaacagaaaa atgcttaatc
    13801 gcaagtgaac tataacggtt cactttttgt ttacaattcc tttacgccgc tgtaaagttt
    13861 tcctcagatt gtcttttaat accttataaa tagtgatcta tccctaatgt caaatacatt
    13921 tgatagaccc gttagcttat catccttata ctaatattat gaaaggagat atttatggaa
    13981 attggtcagc aaattattcg ctaccgtaaa caacaagcgc tctcgcaaga aaaattagcc
    14041 gagaaagtct atgtaagccg tcagagcata tctaactggg aaaatgacaa aacttaccca
    14101 gacatccata gcctcttact cctaagtcaa atttttcaag tgagcttaga ccaactcatt
    14161 aaaggagaca ttgagaaaat gaaatatacc atcactcagg ttgataaaaa gaattttaaa
    14221 cgagatacca aagtaatggt aacattgatg atactattaa tgatttcaag ttatccatta
    14281 gtctatttct tagagtggct aggacttggc atttttgttt tattatcgat aattactatg
    14341 acttatgcta atcgtgtgga acgttttaag aaaaaatatg atgtgcaacc ttataaggaa
    14401 atcctagctg tttcaaatgg aaaattatta gacgaaatcg aaaaatgaga agaaagagca
    14461 aaacttcctt atcagaaacc ccttattgtc actgtatttt tcctcattac tgttgctttt
    14521 gcttttgcaa gtcgttttat gtttacatgg ttattccatt gaagttagta ggtcataaaa
    14581 taaagctcag cgtgacgctg ggctttattt tagttaactc aattcagcaa taatagcttt
    14641 cagttggtct ttagtatgaa cacctgcaac ttgtttaacc acttctccgt cttttttaaa
    14701 catcaatgtt ggaattgaca taataccaaa ctgacgagca gtttccggat tttcatcaac
    14761 atccattttc aaaattttga gttcatcttc atcgatttct tgagataact gttctaagat
    14821 tggtgcttgc atacgacatg gaccacacca agttgcccaa aaatcaatga gtactaaacc
    14881 ttctttagtt tcttctacaa atgtagcatc tgttacttct aatgccatct tttttctcca
    14941 tttcatctta atgataagcc tattgtacaa gaatttagtt gtttttgaaa ctatttgcta
    15001 cgaactcgct aaatcaataa acaaagccct aacatcaagc caataaggta acctacaata
    15061 acatccttag gatagtgaat acctgctatc acacggcaaa ttgccaataa aaccgataat
    15121 attaaacaaa ctattccaaa gtaaacataa tatcgtaaca aacacatact aatcatcgta
    15181 gctgaaaaaa catgtctgct aggcattgac cttccctttg tatccttatc aattaaaggt
    15241 ttaatattcc atttttcata cggtcgtgga aaatcaaagc gtttcctaat gtaagacaaa
    15301 gctataaagc caacagcagg aataattaat aaccttaacc ataattgaaa tgtcatccct
    15361 tgccaaagcg taaatatcag aaaagatgga taaagtatgt acatcactcc tgtgattatt
    15421 tttagtaaaa catttaaaag aattattaac tgtggggttt ttctaaaagg ttgagatagt
    15481 ttggcataaa agtgttcata tgattccatc atttacccta acgttacaat tgtggcacca
    15541 gatccccctg cattttgtgg ggcataagca aaatgcttaa catgcttatt acgacgaaga
    15601 tattttgtca ctccctcacg aataacgcct gtaccaatac catgaataat atcaacttgt
    15661 cccatattgt taagcaatgc ttgatcaata aaatgatcta actcttgcat tgcttcttcg
    15721 tatcttttac ctctaagatc aagtcgagct cttggtccag aactatcagc ctttttaacc
    15781 acattaatct gtttattttt gactttctgt tcttcttgga ctctaacgag gctaaattca
    15841 tcttgtgtta atgtcatttt gataattccc acttgtgctt cccaacggcc atcttttaat
    15901 tgactagtta aggtacctcg ctgtccataa ctagtcacta taatatcatc accaattcta
    15961 ggggcacgag ctgctttgat ttttttagcc ttatttaaga ctttattttt tgataaatca
    16021 acttgaggtg ctaatttttt tatttgtgcc ttagcatcta taatttcgtg aggttttaat
    16081 tggctcttat cattgagttt ttttaagata gtatcactct cattcaaagc catatctaca
    16141 atttcttgag cttcttgata gattttttct aactctttat cgcgttcatg tgaaaattca
    16201 ttatagagtt tcttaaccgc acgattgaat ttgagatttt cttgttcaac ttctttaata
    16261 tgatccagtc ttctacgtgt ctcaagtgtc tgtgcctcta actgttcaat aatacggtta
    16321 acatctgagt cagaatctgt catctgctta gcttgtttaa caataaatgg agctaaacca
    16381 aggcgagaag caatttcaaa tgcatttgag cgtccaggaa ctccttgcat aaagcgatac
    16441 gtaggactaa gcgtttctgc atcaaattcc atgctcgcat tctctacaaa atttgtttca
    16501 atcccataag cttttaattc tggatagtgc gtggtcgcca tcgttttgat atgacttaac
    16561 ctaaggtgtt ctaaaatagc catagccaaa ctagcacctt cttgaggatc cgttcctgct
    16621 cccagttcat caaagagaac taaactattg cggtcagcct cgtttaaaat actgactatg
    16681 tgcgtcatat gactagaaaa agttgataga ctttgttcaa tagattgctc atcgccaata
    16741 tctgcaaaga tattgttaaa tactgtaatt ttactacctt tatccgctaa tactggcaaa
    16801 ccagactgtc ccattaattg tgctaaaccg agtgttttta gcataatcgt cttaccacca
    16861 gtattgggac cagtgatgac aattgcagtt aaatcgtgat caaaatgtaa gtcattagcc
    16921 acaggattac ttaacagagg atgacgaaca ttgattaatg ctaacgtgct gtcatcagag
    16981 atcttaggta tcgtcgcctt attatcagac ataaaaagat atttagccct tacaaaatca
    17041 agatgcccaa gaatccatgc attatttcta atagtggcga catgaggtct taacaagtct
    17101 gaaaatgtgt gtaaaatacg accttcttcg tggcgttcgt cagctctaag ctgcgttatc
    17161 tcttcgttta gtgtaactac agcacgaggc tcaatataaa cagtacttcc tgaagaagag
    17221 atgtcatgaa ccacaccaga aatacgattc cgataagtat tttttactgg taggacactt
    17281 cgtccactac gactagcgat tagattctct gataaaagct ctgctttttc cttaagcata
    17341 tcctgtaaaa tctgacgaac ccgtcgttca ctatttgtta attgacgacg gatacgctct
    17401 aattctggac tcgcaaaatg ttctaaaaaa ccaccatcat tgatagcttg aaaagaccct
    17461 tgcagattag gtaattgttc caaattttca aacaaacgat ccaaagattg gaaagaaaca
    17521 ttatccaaat cagaataaaa gtgaatcata tccgaagaac tctgtaaaac ttttttgata
    17581 gccaaaagct cttgaatatt aagatcagct gaaagctcta agcgttttaa actctcagag
    17641 attgaactta ggctgactat gccaaatgag tgatgttcaa caaaaatctg ttccatgtca
    17701 gaaatttcat taaaactttt ttctatttta ggagcctccg tcatcggctc cagctctaat
    17761 aattcttctt gtgattgttc tgtcttgaga taaggtaata gcaattcctt aactttgtta
    17821 aattctaact gctctaaaat cttgttattc atactcttca ttatacctaa aatgatggaa
    17881 aatggggcaa gaaagtcact aagttcccat gcgttctctt tatttctaaa cagtttctcg
    17941 taaataaaga acctaaaggt gaaactcact ccaggttctt atattagtca caaaacgata
    18001 tcaaataatg gcttgaatcc acaatttttg aataattatt gtcaatggtg gaaggtgttc
    18061 gatcaccaaa cgagcaagaa aactagagtg caaatagtgc tgtaaaaaag gcatagggac
    18121 cgttgcaaaa atagacagta acatgttcaa aaaaagaagc gatactagaa gcgctagtcc
    18181 gccactcaaa cattttgtcc actggttatc aaaataatct agcaaaagga aatgaacaaa
    18241 aatcccaaga aagcgactca atgcataccc caataagaaa atgataaaaa aggcaaggcc
    18301 tgcataataa accttatcta aaacaaagat atcaactgat ttaaagaaaa agactgatgt
    18361 tccttctacc gggttagaat aaggaatcca aagcgttagc ttatgggcta atccaatata
    18421 aaatcgatta gctaccaaaa gagaaagtag ggctcctaag acataaaacg attgaagaat
    18481 aatacctcta ctataaccaa tataaaaatt ccaagttaaa acaatcacta tcaataaaga
    18541 taacatgatt aaacctcatt cacatctatt tgattagctt tttcttgaag accaaccaag
    18601 gttttttgac gtaaatctaa aatttcagct tccatttttt caatggcaat ctctctgctt
    18661 aattgtgttg acaaggtatt gatagccatt aaaatagcga tagtctcgtc atcagcttct
    18721 ggtaaatgat ttttaagagc ttgatacttt tctttagcta cccgctcaac ctcttccata
    18781 aagagattgt ctttgtcagt tgtcaatgtt aatgtttttt caccaaaggt gaatttgtag
    18841 cgattaatac ttttcatagg gtcacctcat tagcattata ccgtaaaatg aaactttcgt
    18901 aaaatagtca acac
//
LOCUS       DAUCWU010000025        18207 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUCWU010000025 DAUCWU010000000
VERSION     DAUCWU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.025"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..964
                     /locus_tag="VGY24_000830"
     CDS             <1..964
                     /locus_tag="VGY24_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ4283828.1"
                     /translation="DLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQEGDGFGGVT
                     IDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLYGEL
                     AQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLNLFSYTAAFS
                     VAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRKKLI
                     FDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQFKK
                     QIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            961..1647
                     /locus_tag="VGY24_000831"
     CDS             961..1647
                     /locus_tag="VGY24_000831"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ4283829.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1741..2907
                     /gene="aroC"
                     /locus_tag="VGY24_000832"
     CDS             1741..2907
                     /gene="aroC"
                     /locus_tag="VGY24_000832"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ4283830.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            2968..3309
                     /locus_tag="VGY24_000833"
     CDS             2968..3309
                     /locus_tag="VGY24_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ4283831.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPMAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3530..4882
                     /gene="gorA"
                     /locus_tag="VGY24_000834"
     CDS             3530..4882
                     /gene="gorA"
                     /locus_tag="VGY24_000834"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ4283832.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(4969..6237)
                     /locus_tag="VGY24_000835"
     CDS             complement(4969..6237)
                     /locus_tag="VGY24_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ4283833.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVIAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLAKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6267..6707)
                     /locus_tag="VGY24_000836"
     CDS             complement(6267..6707)
                     /locus_tag="VGY24_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283834.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNS"
     gene            6942..8084
                     /locus_tag="VGY24_000837"
     CDS             6942..8084
                     /locus_tag="VGY24_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ4283835.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVKTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPSGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8096..9310
                     /gene="thiI"
                     /locus_tag="VGY24_000838"
     CDS             8096..9310
                     /gene="thiI"
                     /locus_tag="VGY24_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ4283836.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9348..10640
                     /locus_tag="VGY24_000839"
     CDS             9348..10640
                     /locus_tag="VGY24_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ4283837.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     VTVVALIVAFLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDNIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10745..10819
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            10854..11168
                     /gene="rplU"
                     /locus_tag="VGY24_000840"
     CDS             10854..11168
                     /gene="rplU"
                     /locus_tag="VGY24_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ4283838.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11180..11506
                     /locus_tag="VGY24_000841"
     CDS             11180..11506
                     /locus_tag="VGY24_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ4283839.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11534..11827
                     /gene="rpmA"
                     /locus_tag="VGY24_000842"
     CDS             11534..11827
                     /gene="rpmA"
                     /locus_tag="VGY24_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ4283840.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12175..13089
                     /locus_tag="VGY24_000843"
     CDS             12175..13089
                     /locus_tag="VGY24_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4283841.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13086..13544
                     /locus_tag="VGY24_000844"
     CDS             13086..13544
                     /locus_tag="VGY24_000844"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ4283842.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMIHLDFMDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13534..14424
                     /locus_tag="VGY24_000845"
     CDS             13534..14424
                     /locus_tag="VGY24_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ4283843.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            14820..15341
                     /gene="pyrR"
                     /locus_tag="VGY24_000846"
     CDS             14820..15341
                     /gene="pyrR"
                     /locus_tag="VGY24_000846"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ4283844.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15357..16616
                     /locus_tag="VGY24_000847"
     CDS             15357..16616
                     /locus_tag="VGY24_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ4283845.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16677..17612
                     /locus_tag="VGY24_000848"
     CDS             16677..17612
                     /locus_tag="VGY24_000848"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ4283846.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17656..>18207
                     /locus_tag="VGY24_000849"
     CDS             17656..>18207
                     /locus_tag="VGY24_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEQ4283847.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSI"
BASE COUNT         5531 a         3152 c         3970 g         5554 t
ORIGIN      
        1 cgacttgtct atctcttatt ttgttagtct attttcagtt gctaaggcca aaagacaaga
       61 ttttgctcaa tcggacgaga ccaatgctta tcgccttttt aatcaagaag gagacggttt
      121 tggtggagta acaattgatc tttataaaga ttttgcagtt ttttcatggt acaatgcctt
      181 tgtctatgac aaaaaagaaa tgattatgga ggcttttcaa caggtttttc ctgaagtcaa
      241 aggagcttat gaaaagtgtc gctttaaagg tccagatact gaaactgctc atctgtatgg
      301 tgagctggca caagaaacct tttcgatttt agaaaatggc atagcctatc aagtcttctt
      361 gaatgagggc ttaatgacag gtatttttct agaccagcat gatgttagaa gagcactagt
      421 agatggttta gctatgggca agtcccttct taacctattt tcctatacag ctgccttttc
      481 tgtagcagct gctatgggtg gtgctatcga gaccacttct gtggacttgg ctaaacgatc
      541 gagagaatta tcactagctc attttgagca taaccagtta aacttagcct cacatcattt
      601 tgtggtgatg gatgtttttg aatacttcaa atatgccaaa cggaaaaaat taatctttga
      661 tgtgattgtg attgacccac caagctttgc tagaaataaa aaacaaactt tctctgtgag
      721 cagggactac cacaaattaa tcacagaggc cttggacata ttgagtccta agggaactat
      781 tatagcttca acaaatgctg ctaatatgac tgttagccag ttcaaaaagc aaattatcaa
      841 aggatttgga agtcgtcgtc ctgagtctat gactctccag cagcttccga gtgactttac
      901 tatcaataaa gcagatgaaa gaagtaacta tttaaaggta tttactataa aggtacgaga
      961 atgagaattg tagcaccagt catgccaaga cattttgacg aggctcaagc cattgatatt
     1021 tctaaatatg aagatgttaa tttaattgaa tggcgagcag actttcttcc caaggatgag
     1081 attgtcgcag tagcaccagc tatttttgaa aaatttgccg gaaaagaaat tatctttacc
     1141 cttcggaccg ttcaagaagg ggggaatatt accctttcaa gtcaggagta tgttgatatt
     1201 atcaaagaaa tcaatgctat ttataatcca gactatattg actttgagta ttttacgcac
     1261 aagtcagtat ttcaagaaat gcttgatttt ccaaatctta ttttgtctta tcacaacttt
     1321 gaagagactc ctgaaaattt aatggaagct ttttcagaaa tgaccaagtt ggcaccgcgt
     1381 gtggttaaaa ttgctgtgat gccccagagt gaacaagatg ttctagattt gatgaactat
     1441 accagaggct ttaagacgct caatcctgag caagaatttg caactatatc tatggggaaa
     1501 ttaggaagat tgtctcgttt tgcgggagat gtcattggct cttcctggac atacgtttca
     1561 ttagatcatg ttagcggtcc aggtcaagtg actctcaatg atatgaaacg tattattgag
     1621 gtcttagaaa tggatatctc taactagtga atggtctaaa aatattattt ctacaatagt
     1681 taagaaatga tgtttttaga ttaatgtttt taaaataagt ggaaccaaaa aggagaaaaa
     1741 ttgcgttatt taacagcagg agaatctcat gggccatcct taacagctat tattgaaggt
     1801 ataccagcag gattaactct tcatccagcg gatattgatc acgaattaca gcgacgtcaa
     1861 ggaggttatg gccgtggtgc cagaatgtct attgagactg atcgggttca aatatcctca
     1921 ggagttcgtc atggcaaaac aacaggggcc cccattacct taacggtaat taataaggac
     1981 caccaaaaat ggctagatgt catggctgta ggagacattg aagagactct caagttaaag
     2041 cgtcgtgtta agcatcctag accaggacat gcggatttgg taggaggtat taaatatcat
     2101 tttaacgatt taagagatgc tttagagcgt tcctcggcta gagaaacaac gatgagagtg
     2161 gctgtaggcg ctgttgctaa acgtatctta gctgaacttg gaattgatat gctacatcat
     2221 atcttaatat ttggtggtat aacgatcaca ataccttcta agctctcttt tagagagttg
     2281 caagaaaggg ctctccattc agaattatct attgttaatc caaagcaaga agaagaaatt
     2341 aaaacgtata ttgataagat aaaaaaagaa ggggatacta ttggtggtat tatcgaaacg
     2401 atagttcaag gggtgccagc aggtcttgga tcttatgtcc aatgggataa aaaattagat
     2461 gccaagctgg ctcaagcagt tctttccatt aatgctttta aaggggtgga atttggagtt
     2521 gggtttgata tgggattcca aaagggctct caggtaatgg atgaaatcac ctggacacct
     2581 acccaaggct atggtcgtca aacaaaccac ttaggtggct ttgaaggtgg catgaccaca
     2641 ggacaacctc ttgttgttaa aggggttatg aagcccattc ctactttgta taaaccactc
     2701 atgtcggtag atattgatag tcatgaacct tataaagcaa cggtggaacg ctctgatccg
     2761 acggccttac cagctgcagg ggttatcatg gaaaatgtgg tggcgacggt gcttgccaaa
     2821 gagattttag agactttttc ttctactacc atgtctgaat tgcaaaaagc tttttcggac
     2881 tatcgtgcct acgtcaagca attttagata gcgagatact ggcttttatg gtatcataca
     2941 ggtattaatg aacaaaagga gactgtcatg tcacaagaaa tttatgacta tgctaatcaa
     3001 cttgaaagag cagttcgtgc tctgccagaa taccaaaaag tgttagaagt aaaagaggcc
     3061 attcaagcag acgctagtgc tagcgaactg tttgatgagt ttgtcgctat gcaagaaaaa
     3121 atccaaggga tgatgcaatc aggccaaatg ccaatggctg aagaacaaac aagcattcaa
     3181 gaacttagcc aaaagattga agctaatgac cagctaaaag cttattttga agcgcagcag
     3241 gccttatctg tttacatgag tgatattgag cgtattgtat ttgctccgtt gaaagattta
     3301 gtcaagtaag ccaattaaga taggaaaaaa acagattgct ggcagctcgt caggatctgt
     3361 tttttagtgt ttattttctt tgtatcagaa agttgagcct ctttcttatt tgtgatatag
     3421 tcaaataaag tgaccaaaat agcaggccag tagtggcttt aatgaaggca agttgtctgc
     3481 taactggcta tgctaaaaca tcagtattag aaagtaaaag gtgataaaaa tggttattcc
     3541 ttatgattat attgttattg gtggagggag tgcaggtatt gcttctgcga acagggcagc
     3601 catgcatggg gccaaagtat tgttagctga aggtaaagag attgggggaa cctgtgttaa
     3661 cctaggctgc gttcctaaaa aagtcatgtg gtatggcgct caagtagcag atattttagg
     3721 gacttacgct aaggattatg gatttgactt taaagacaaa gcatttgatt ttaaacaatt
     3781 gaaagcaaat cgtcaagctt atattgatcg gattcatgct tcttatgagc gaggctttga
     3841 gcagaatggt gttgaccgta tttatgatta tgctgttttc aaagatgctc atacggtaga
     3901 aattgcaggg cagctttata ccgcaccgca tattttgatt gccacaggag gacatcctgt
     3961 ttttccagat attgaggggg cacaatacgg tatcagctcg gacggctttt ttgccttaga
     4021 tgaggtacca aaacgtacgg ctgtggttgg ggcaggctat attgctgtgg aacttgctgg
     4081 tgttttgcat gctttgggat cgaagacgga tttgtttatc cgtcacgatc gacctttaag
     4141 aagttttgac aaagccattg tggatgtttt agttgaggaa atggcagttg gtggtccttg
     4201 cttacaccct cattcagaag tggcaaaggt tgtcaaaaat actgatgagt ccttgaccct
     4261 ttacctcaaa gatggccaag aagttgaggt tgaccagctc atttgggcta tcggtcgcaa
     4321 gcctaatcta gaaggttttg gtcttgacaa aactggagtg acgttaaatg ataaaggtta
     4381 tattgagaca gatgcctatg aaaacacgtc tgtcaaaggt atttatgccg tgggagatgt
     4441 taatgggaaa ctggctttaa ctccagttgc tgtggcagct ggacgtcgcc tatcagagcg
     4501 tcttttcaat ggcaaaacag acgagaaatt ggactaccaa aacgttgcta ctgtgatttt
     4561 tagtcaccct gttattggct ctgttggctt gtcagaagaa gcagcagtca aacagtatgg
     4621 gcaagaagca gttaaaacat atcaatcacg ttttacgtct atgtttactg ctatcactaa
     4681 ccatcgccaa ccttgtctga tgaagttggt aacagtcgga gataccgaaa aaatagttgg
     4741 tcttcacgga atcggctatg gggttgatga aatgattcag ggctttgcag tagctatcaa
     4801 aatgggagca accaaggcgg attttgataa caccgttgct attcacccaa ctggctcaga
     4861 agaatttgtc accatgcgct aaaaaatgta gcctaaggca ataaaaaatg atgttaacag
     4921 tttttgagga aagtcttcta aactgctagc atcgtttttg ttaggtgatt aggggaatat
     4981 gtttttaaag atatttgcgt tgctcagaca atattgcctt acctcggaga taaaataaag
     5041 tgaccctgta acaacaaaga gttcatcaga tgttacggaa ccttgtaacc agtctctgta
     5101 atcttttacc tgtgggtatt tatcaggata atcctctaaa agatttgctt cagggaaatc
     5161 aaaagtcgtg acagatactt caaaagtatc tagttgagcc agcaagtcgg ctaaaggctt
     5221 acgtttgaga cctgcaaaga gaatatgaat aggacgtttg ggaaattctt cttgcagtag
     5281 agactttaat ttagcaatgg aatctggatt gtgagcgccg tctaatagaa gattaggttg
     5341 aataaattcg gaccggccag gccaagttgt tgcctcaatt cctttttgaa tcgtttttgg
     5401 agatatttta ggaaaggatt ttctaagtaa ttgagctgtc ataatggcta aacaggcatt
     5461 cgctttttga tgttgaccta atagtttgag acggagtttg gctaaattca agttatcgtg
     5521 ttgaaaggaa aacgtctttt ctgaaggatt gagcaaaaaa tcttttccta attgatataa
     5581 gcgtgaaccg gctttttggg taacttgttg aaagacttga ctagcctcct gctcaagttg
     5641 accgataata acgggttctt tagctttgat aacttctgct ttttggtgag caatttctgc
     5701 taggctataa cctaaacgtt cttggtgatc aaaactaata gaagggcata cgacggccag
     5761 agcatgaaaa acattggttg cgtcggtttt cccaccaatc cctgcctcaa taatggcaat
     5821 atcaacaggt ttaagattgg caaagtagca aaacattatg agcgtcacta actcaaactc
     5881 ggtaggtcta tcccaatttg tttcaaggga cattttagtg agaatgggtc taatcacgct
     5941 cacacattgc gccaattcta ggtctgaaat gggctggcca ttaaggcaaa tccgatcgtg
     6001 aaaagagata ataaaaggag atgtaaaggt tcctacctga taaccagaag ttgacaggat
     6061 atgctgtaag taggctatca cagagccctt gccgttagtt ccaacaacgt gaatagcagg
     6121 gaatttgtct tgtggccggc ctaatgcctc aagtaaccaa gccattctgg ccaaatcggt
     6181 cttgcgacca ttagccttaa aggtatgaat ccaggaaatg ctatcatagg tagtcattac
     6241 tatttcttgt ccttctaaca atgcttttaa gagttcgtag ggagaccagt attagcatcc
     6301 tcagctccag ttgcattagg gagttgcgtc ccttcagtgg ttgatggtgt ctcaggagct
     6361 gtaggagttg tctcgttgtg agaaggtgtg gtagaagatg acggaagagt tgtcttatta
     6421 tcatctgtgg tttctgaagt acctgtatgg ttttgattac cctgagaaga ctctttttca
     6481 ataatcttga caatggtatt actcgattct ttgggtgtat cattagaggt cttatctggt
     6541 acaggctgca gtgctcttgt aatggtaatg gttgccacaa tgatactcaa aatactacca
     6601 ataagtgcaa tggatttttg gaaagctgta aaacctgttt taatatgctc ggtagggaca
     6661 cctcctgatt tacgaggttt ttgttgacgg gaataacgtg atgacatatt tttctccttt
     6721 aaaatcatcg taatgccaag attaaggagg aggaaacaga agtcctagct cctctttaat
     6781 acttcatcta tcagtataat ctagaaaaaa agtgaagtca acggtttacc ttatgtctag
     6841 gacaaggaag tcctttgggc ttattgtaat atgaatattc ttgatttcat tttagctatt
     6901 ttgtgataga ataaagtatt gagtaaaaat gaaggtgaca catgatttat tttgataatg
     6961 cagccacaac cataccttat ggagaggctc ttaaaactta ccaagaagta gcaacaaaaa
     7021 tatatggcaa tccctcaagt ctgcaccaat tagggacaaa tgcgtctcgc attttagaag
     7081 cttcccgtaa acagattgct ggtttgttgg gtgttaagtc agaagaaatc ttttttacgt
     7141 ctggtgggac tgaaagtgct aattgggcta ttaagggaat tgcttttgaa aaaactgctt
     7201 ttggcaaaca tattattatc tcagccattg aacaccctgc cgtgagtgaa agtgtcaaat
     7261 ggcttctcac acaaggattc gaagtttctt atgctccagt gaaaacacag ggagttgttg
     7321 atgtgaatgc tttagctgag ctgattagac cagacaccat cttaatctcg attatggctg
     7381 ttaataatga aatgggagct atccaaccga ttagagccat ttcaaaccta ttggctaacc
     7441 aaccaacgat cacctttcac gttgatgctg ttcaagccat tggtaaaata ccactctgtg
     7501 attatatgac taatcgtgtg gatttggctt ctttttcagg gcacaaattt catagtgttc
     7561 gtggtgttgg cttcctttat aaaaaagcag gcaagcgact caatcctctc ttgtcaggcg
     7621 gcggccaaga gcaggagtta cgttcaacca ctgaaaatgt tgcaggaatt gctagcatgg
     7681 ccaaagcatt aagaatagtc actgaaaaac aagtgagcgt cttacctaaa ctaacagcga
     7741 tgcgagatgt gatttacaaa gctttgtccg cttacccaga tgtgacagta ttttcagctc
     7801 aagaaggctt tgcgcccaat attttgacct ttgggattag aggggttcga ggagaagtta
     7861 ttgtccacgc ctttgaaaaa tatgagattt atatttcgac cacaagtgca tgttcgtcca
     7921 aagcaggtaa gccatcagga agcctagtgg ctatggggat tcctgtaaaa actgcccaga
     7981 cagccgttcg tattagtcta gacgatgata acgatatggg acaagtagag caatttttga
     8041 ccatattcaa acagatttat gataaaacac agaaagttag gtaagattag gtaagatgga
     8101 ctattcagaa attatggttc gccatggtga actatcaact aaagggaaga atcgcatgcg
     8161 attcattaac aaacttaaaa acaatattca agacgtactg gctcctttcc cagccattac
     8221 tgtccgttca gatcgtgata ggacccatgt gtctctaaat ggcacagatt accagcctat
     8281 tgtagaagct ttaaagctgg tgtttggtgt tcaagctcta tctcctgttt ataaacttga
     8341 aaaaagtgtc cctcttttgg taacagctgt tcaagatatc atgacttctc tttatcgtga
     8401 tggactgacg tttaagattg ccactaagcg tagtgatcac gcgtttgaat tggatagtcg
     8461 tgagcttaac agtcttcttg gtggagctgt ttttgaggtt ttaccaaata tccaagccca
     8521 gatgaaacat cccgatgtga ctctcaaagt cgaaattcgt gatgaagcag cttacatttc
     8581 ttatgaagag attaagggag caggcgggtt accagtagga acgtcaggga aagggatgct
     8641 gatgttatcg ggcggtattg attcaccagt agctgggtat ttagccctta aacgtggcct
     8701 tgacattgaa gttgttcatt ttgctagccc tccgtacacc agtccaggtg ccttggctaa
     8761 agcccaagac cttactcgtc ggttaactcg ttttggtgga aatatccaat ttatcgaggt
     8821 tccttttaca gaaattcaag aagaaattaa aaataaggca ccagaggctt accttatgac
     8881 attaacccgc cgctttatga tgcgcattac agatgccatt cgtgagcaac gtaagggtct
     8941 tgttattgtt aacggtgaga gcctaggtca ggttgctagt caaacccttg aaagcatgca
     9001 ggccattaat gcggtaacct caaccccaat tattagacca gtcgtgacaa tggataaact
     9061 tgaaattatc gagatggctc aggcgattga tacttttgac atttctattc aaccgttcga
     9121 agattgctgc actatttttg caccagaccg tcctaaaacc aaccctaaac taggtaatgc
     9181 tgaaaaatat gaagaacgct ttgatattga tggtcttgtc cagcgtgctg tgtcaggaat
     9241 tgttgtcaca gaaatcacac ctgaaatcgt caatgatgag gttgaaaatc tcattgatgc
     9301 tttactttaa aagaaaagat caagtgtaaa tcttttagta gtcagtaatg atactattga
     9361 aaagcctcta taagggcttt tttgctgcaa tgaaggagga aaattttgag gcaagatgtt
     9421 ataatagaga aaaaggaggg tgtgtcttga ttaaaactat gcaatacaaa aaaacgatgg
     9481 taacagttgt tgctcttatc gtcgcatttt tgctatttgg cttgatttat gatttacttg
     9541 gggttcaaaa aaataagcta gcagctcaaa aatctgctca gccaaaggtc aaaacagcgc
     9601 gtgttgtagc taatggcgac attttaattc atgatattct ctacatgagt gctagaaaag
     9661 ctgacgatac ctatgatttc actccttatt ttgaatatgt taaggactgg attagcggtg
     9721 ctgacttagc tattggagat tatgaaggga ccattagccc agattatccc ttagcaggct
     9781 atcccttatt caatgctcca gaagagattg caggtgctct gaaaaatact ggctatgatg
     9841 tagtcgattt agcccataat catatcttag attcccaatt agacggtgcc cttaacacta
     9901 aaaaggtttt tcatcaatta ggtatagaca gtattggcat ctatgacaag gatcgttcaa
     9961 aagaatcctt cttaattaaa aatgtcaatg gtatcaaaat tgcaatttta ggttattctt
    10021 atggttataa cggcatggag gccacactta gccaagaaga ctatgagaaa cacatgtctg
    10081 atttagatga agctaagata aaaaaagaac ttcagctagc tgaaaaaaag gctgacgtga
    10141 ccattgtcat gccacaaatg ggaacagaat atgccttaga accgacagca gagcaaaaag
    10201 aactttacca caaaatgatt gattggggag ctgacgtcgt cctaggaggc catccgcatg
    10261 ttattgaacc ttcagagaca gttataaaag gtaggcaaaa gaaattcatt atttactcta
    10321 tgggaaattt tatttcaaat cagcggcttg aaaccgtaga tgatatctgg actgagcgtg
    10381 gcctattgat ggaccttact tttgagaaga aagataacaa aacaaaaatt aaaacagttg
    10441 aggcccatcc aaccatggtt ttagccaaag gtaaaggcat tgtgggtaaa gaaggttttg
    10501 aactatacaa ttatcgaaca atggttttag aagattttat caaaggtgga aaataccacg
    10561 acaatattga tgaggagacc aaagcaaaag tagcacttgc ctatcaggaa attaatgatt
    10621 tagttaacct gaaatggtaa tgtttggtgg tttagcatct aacgataaaa aatagagatt
    10681 aaacttaatt tttccttgat aaaaagcagt tatggtgcta aaatagtaac gttgactaaa
    10741 tgcacatcct gtgcaaccgc gcgaaaatcg ttatcaagta gaccaagtct cacgattcca
    10801 ggcgagtctt cacagaggga aacctcaaac aaaaatttta taggaggtgc atcatgagca
    10861 catacgcaat catcaaaact ggtggaaaac aagttaaagt tgaagtaggt caagcaatct
    10921 acgttgagaa aattgacgct gaagctggcg cagaagtcac ttttaacgaa gttgttcttg
    10981 taggtggtga caaaactgta gttggtactc cagttgttga aggagctact gtcgttggaa
    11041 ctgttgaaaa acaaggaaaa caaaagaaag ttgtaacatt caaatacaaa cctaaaaaag
    11101 gtagccaccg taaacaaggt catcgtcaac cttacactaa agttgtcatc aacgcaatca
    11161 acgcttaatt tactagctta tgattaaagc aatttttact cgccaaaaaa atggtcaact
    11221 gagcagtgtt acactgacag gtcatgctgg tagtggtaaa catggctttg atattgtttg
    11281 tgcctctgtt agtactctag ccattaactt tgttaattct cttgaggtgt tagcagattg
    11341 ccaagcatta gtagatctta atgatgttga aggtgggtat atggctatta caataccacc
    11401 acatgacaac aaggaggaag ttcaattatt atttgaatct tttcttctag gaatgactag
    11461 tttggcgaag gactcttcga aatttgtcaa cactcaggtc atctaaattt aatttaagag
    11521 aggaaacaac attatgttaa aaatgaatct tgctaacttg caacttttcg cccacaaaaa
    11581 aggtggaggt tctacatcaa acggacgtga ctcacaagct aaacgccttg gtgctaaagc
    11641 tgctgacggt caaactgttt caggtggatc aatcctttac cgtcaacgtg gaactcatat
    11701 ctacccaggt gtaaacgtag gccgtggtgg agatgacaca ctttttgcta aagttgaagg
    11761 tgttgtacgt ttcgaacgta aaggacgcga taaaaaacaa gtttctgttt acccagtagc
    11821 taaataattg tttaacatcg cattatataa ggctttccag gtgtctggaa agccgatttt
    11881 tttattttgc tactcatttg ctacccatat tctaaaagcc gatgtaggaa gagagcttgt
    11941 cagcagtttt atcttttgag gatttagtaa catgagcgta gatattcatg gtagtttgga
    12001 tagtttcacg accaagtgta ttggattttc ttctatccta ctaaagattg tagttgctta
    12061 tttagagcaa tagttctaat tgtaaaaagt cagcataata aaacaatatc ataagatgtt
    12121 gttataacgg gctttatggt ataattaagg atactcattt taggaaggaa agcaatgaat
    12181 attcaacaat tacgctatgt ggtagccatc gctaataatg gaacttttcg agaagctgcc
    12241 tctaaattgt ttgtcagtca gcctagctta tcagtttcta tcaaagattt agaggcggaa
    12301 ttagggtttc aaatttttaa tcggactact tcaggaacag tcttgacaag tcaaggacta
    12361 gttttttatg agaaagcttt agaagtagtc aaatcttttg attcttttga aaaaaccttt
    12421 tctcaagctg atctggatca aaatgaattt tctattgcta gccaacatta tgactttttg
    12481 ccacctctta ttacagcttt ttcgcaacaa tacgatggtc atcgtgtctt tcgtatcttt
    12541 gaatcaacaa ctattcaaat tttagatgag gttgctcaag gaaacagtga aattggtatt
    12601 atttacttga atgtagacaa tcaaaaaggc ctctttcagc ggatggataa gctgggcttg
    12661 gaatatgttt ctctaattcc ttttactact catatttatc tttcaaaaac gcatccattg
    12721 gctaatcgcg aagcgcttta tttgagcgat attcaaggat tacctgcggt aaggtttacc
    12781 caagaaaggg acgagtattt gtattattct gaaaattttg tggatactag tgaatgccct
    12841 cgtatttata atgtgtcaga tagggcaacc ttgaacggta ttttagagag aaccaatgcc
    12901 tttgcgacag gatcaggttt tctcgatcat cggagtgtta atgggattaa agtgatacct
    12961 ctagctgatc atattgacaa tcaaatgatt tatgtcaaac gaaaagataa aaacctttct
    13021 gttgctgggg ccacttttgt gactatttta aaagactatt ttgataaaag aaggaaggcc
    13081 ttaacatgaa aaaacgattg tttgtgctta gcttgatcct ccttgtagct ttggatcaac
    13141 ttagtaaatt ttggattgtt tctcatatag cgcttggaga agtgaaaccc tttatcccag
    13201 gtatcgtcag cttgacttac ttgcaaaaca atggggctgc cttttccata ttgcaggacc
    13261 agcaatggtt ctttgttgtc ataacggttt tagttatcgg ttatgctatt tattaccttg
    13321 ctactcatcc ccatttaaat atctggaaac aattagctct cttgcttatt atttctggtg
    13381 gaatcgggaa ttttattgat cgtttgcgtt tagcttacgt gattgatatg attcatttag
    13441 actttatgga ttttgccatt tttaatgtgg cagattcata ccttaccgtt ggtgtcatat
    13501 tattagtgat atgtttatgg aaagaagagg attatggaaa ttaatgtgat aacatcgggg
    13561 caacgcttgg ataaggcact agctgattta agtcccctgt cacgtggtca agccaatgac
    13621 caaattaagc aaggccttgt tttagttaat ggtcagcaaa aaaaggctaa atatacggtt
    13681 caagcaggag atgtgatttg ttttgaactc cctaaagaag aagtcttaga gtaccaagct
    13741 cagaatattc ccttggacat tatctatgaa gatgatgctc tcgccattat taacaagcca
    13801 cagggaatgg tagttcaccc ttcggcggga cacccttcag gaacaatggt taatgccttg
    13861 atgtatcata tcaaggattt gtcctcaatc aatggtgtgg ttcgtccagg aattgttcat
    13921 cggattgata aggacacctc aggtctctta atggtagcta aaacagacgc agctcatcaa
    13981 gccttggcag aagaattaaa ggctaaaaaa tctctcagaa aatacctcgc cattgtccat
    14041 ggaaacctac cgaatgatcg cgggatgatt gaagctccta taggtcgtag cgaaaaggac
    14101 cgtaaaaaac aggctgttac agcaaaaggc aaagaagcag tgacacgttt tacagtgcta
    14161 gaacgttttg gcgattacag tctagtggaa ttacaactgg aaactggtcg aacgcaccaa
    14221 attcgtgttc atatggccta tattggtcat cctgttgctg gggacccttt atatggacca
    14281 cgtaagacac tatcaggtca tggccaattt cttcatgcaa aaacattggg attaacgcat
    14341 cctatgacag gaaaagaaat gatatttact gttgaagctc ccgaaatttt tcaaaaggtt
    14401 ttaaagctgc taagaaaaaa ataaacagcg aggagtagcc attattttta gtcttatatc
    14461 tttgtgtaaa gtttttaaca ttataaaaaa tactaaaaag catttacgat aagccaggaa
    14521 atatgagaag tcttttatgc tattgggata tgcaacatat ctgttagaga tctttattta
    14581 aactccttaa agaaaagaga tattccaaat agatgctagg tattgtcttt tttacttgtt
    14641 tttcttttct ttttttgcta tactgattga aataaaatat tcctttaaaa cagtcctgtg
    14701 aggctgtcaa ggagacggat taaaaacatg cactatgata cttaattgtg ctatattttg
    14761 ctgaatctcc ttgtttccaa ggagatcttt ttatttgaat ttttagagga ggtttcaaca
    14821 tgaaaactaa ggaaattgtt gatgacgtga ccatgaaacg cgccattacc cgtattactt
    14881 atgaaattat tgaacgtaat aaacaattag acaatgtggt tttggcaggc atcaaaacga
    14941 gaggtgtttt tctagctcgt cgcattcaag aaagactgca tcaattagaa gggttggatt
    15001 taccaattgg tgagttagac atcaagcctt ttagagatga catgagagtt gaggaagaca
    15061 caactctcat gtcagtagat attacaggca aggatgtgat tttgattgat gatgtgcttt
    15121 atactggtcg taccatccgc gcggccatcg ataacttagt gagtttaggt cgaccagccc
    15181 gtgttagtct agctgttttg gtggatcgtg gccatcggga attaccgatt cgcgcggatt
    15241 acgttgggaa aaatattcca acaagcagtg tagaagaaat tgttgttgag gttgtcgaag
    15301 ttgatggccg tgatcgtgtt agcattattg acccaacata agaaggaggt tttactgtga
    15361 aagatgtcat ttatgatgtg gaagaagtgc caaaggcagg tatgttagtt ggtttgtcct
    15421 ttcagcattt atttgccatg tttggggcga cggttcttgt tcctatttta gttggaattg
    15481 acccgtctgt ggccctccta tcgagtggtt taggaacact tgcacattta tcggtaacca
    15541 aatttaaaat tccagcctac atgggctcaa gttttgctta tatagcagct atgcagttac
    15601 tgatgaagac aaatggtatt ggtgctgtag ctcaaggggc tatgacagga ggtctcgttt
    15661 acctgattgt agctttgatt gttaaagtta ttggcaatga ttggattgat aagattttac
    15721 caccgattgt agttggtccg attgttatgg tcattggtct aagtctagct tctacagctg
    15781 ttaatgatgt catgttaaaa aatgggaact ataatctgac ttatcttgtt attgggttag
    15841 tcaccttatt atcagttatt tttttcaata tttatggtaa gggaattgtt gctattgtac
    15901 cgcttttatt agggctatta gttggttatg ttgtagccct tttagtgggt gttctcaccg
    15961 gtcaagaaat tgttgatttt accaatgtgg ctcaggccaa atggtttagt atcccatcag
    16021 tggaaattcc tttcttaacc tacggggtta aattctaccc aagtgccatt ttaactatgg
    16081 cacccattgc ctttgttaca atgacagaac attttggaca cattatggtc ttaaatagtt
    16141 tgacaaaaag agattatttc aaggatccag gacttgaaaa gactttaact ggtgatggtt
    16201 ttgcgcaaat cattgccggc ttcttagggg cacctccggt cacttcttat ggtgaaaaca
    16261 tcggtgtaat ggctttgaat aaaatattct cggtctatgt tattgcaggt gccgcagtga
    16321 ttgccgctct cctcagtttt atcggtaagg tatcagcctt aattcaatct attccaacac
    16381 cagttattgg aggtatatca gttgccttgt ttggtgtgat agcttctagc ggtttgaaaa
    16441 tcttgattga atccaaagtt gatatggata ataagaaaaa cttattgatt gccagtgtta
    16501 ttttggtctc tgggattgga ggattgatgc ttcaagtaaa tggtcttcaa atttcaggtg
    16561 tcgctttctc aacacttctt ggcatcatct tatatcaggt acttcctgaa aaataggaag
    16621 aattgttgtt agaccactta catagtcatc agtcgttagt gataaagggg ataatcatgt
    16681 cagttgtaaa taatcgcgtt gcacttacta atttagtttc aatggaagca ttaaccaccg
    16741 aagaagtttt aggtcttatc aaccgtggtt cagaatacaa ggctggtaag gttgttattt
    16801 cggatcatca aaaggatctt gttgccaatt tattctttga aaactccacc cgaacccata
    16861 agtcttttga agtggctgag aagaaactcg gcttgacagt tcttgatttc aatgctgatg
    16921 caagtgcggt taacaaggga gaaagtttgt atgatactgt gttaaccatg agtgcacttg
    16981 gaacagacat ctgtgtgatt cgtcacccag aagatgatta ttacaaggaa ttagtagaaa
    17041 gtccaactat tacagcttcc attgtcaatg gaggagatgg ttctggacaa cacccgagtc
    17101 aatgtttact tgacttatta accatctatg aagaatttgg acgctttgaa ggcttaaaga
    17161 ttgcgatcgc tggtgactta actcactcta gggtggcaaa gtccaacatg caaatcctca
    17221 aaagattagg agcagagctt tatttctatg gacctgaaga atggtattca gaagccttta
    17281 atgcttacgg cacttatatt gctattgacc agattattaa ggaattggat gttcttatgt
    17341 tgctgcgtgt acagcacgaa cgtcatgatg gccatcagag tttttcaaaa gaaggttacc
    17401 atcaagcgtt tggattgaca caggaacgtt accaacaatt aaaagattct gctattatta
    17461 tgcatccagc tccagttaat cgggatgtgg aaattgcaga tagtttggtg gaagctccta
    17521 aagcgagaat tgtatctcag atggctaatg gtgtctttgt tcgcatggct attattgaag
    17581 ccattttgaa tggtagaaat aaaaactcat gatgaaagat taaagcatgt ttagaaggta
    17641 ggaaaaggta ggaaaatgac aaaacgatta cttattttag aagacggcac tatttttgaa
    17701 ggagaaccct ttggtgctga tattgatgtg actggtgaaa ttgtctttaa tacaggaatg
    17761 acaggctacc aagaatccat cactgaccaa tcctacaatg ggcaaatttt aacctttacc
    17821 tatcctttga ttggaaatta tgggattaat cgagatgact atgagtccat ttcacccacc
    17881 tgtaaagggg ttgttgtgtc tgaagtcagt cggttggcaa gtaattggcg caagcaaatg
    17941 acgctagatg cgttcttgaa aataaaaggg attccaggga tttcgggaat tgatacgaga
    18001 gctttgacca aaattattcg gcaacacggt accatgaaag ctacgatggc agatgatgga
    18061 gatagtatcc agcatttaaa agatcaactg cgtgcaacag tcttaccgac taacaccatt
    18121 gaacaagtgt caactaaaac agcttatcct gcaccaggga tcgggaaaaa tattgtattg
    18181 gttgattttg gtttaaaaca ttctatt
//
LOCUS       DAUCWU010000026        18070 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUCWU010000026 DAUCWU010000000
VERSION     DAUCWU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.026"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            18..98
                     /locus_tag="VGY24_000850"
     tRNA            18..98
                     /locus_tag="VGY24_000850"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VGY24_000851"
     tRNA            105..175
                     /locus_tag="VGY24_000851"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VGY24_000852"
     tRNA            187..259
                     /locus_tag="VGY24_000852"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="VGY24_000853"
     tRNA            268..339
                     /locus_tag="VGY24_000853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="VGY24_000854"
     tRNA            359..442
                     /locus_tag="VGY24_000854"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            1585..1833
                     /locus_tag="VGY24_000855"
     CDS             1585..1833
                     /locus_tag="VGY24_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ4283848.1"
                     /translation="MCAGMGGGSSDAVTIRALNQLWLLTLSRKDMMDIGIPIGSDVPY
                     CLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST"
     gene            1888..2099
                     /locus_tag="VGY24_000856"
                     /pseudo
     CDS             1888..2099
                     /locus_tag="VGY24_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
     gene            2209..2652
                     /locus_tag="VGY24_000857"
     CDS             2209..2652
                     /locus_tag="VGY24_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ4283849.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2656..3375
                     /locus_tag="VGY24_000858"
     CDS             2656..3375
                     /locus_tag="VGY24_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283850.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3368..4183
                     /locus_tag="VGY24_000859"
     CDS             3368..4183
                     /locus_tag="VGY24_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ4283851.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVFGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILVLTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4223..4606)
                     /locus_tag="VGY24_000860"
     CDS             complement(4223..4606)
                     /locus_tag="VGY24_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ4283852.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWEGITINQENWDLTPFLKHL"
     gene            complement(4657..5913)
                     /locus_tag="VGY24_000861"
     CDS             complement(4657..5913)
                     /locus_tag="VGY24_000861"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ4283853.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6005..8317
                     /locus_tag="VGY24_000862"
     CDS             6005..8317
                     /locus_tag="VGY24_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ4283854.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8581..12147
                     /gene="rpoB"
                     /locus_tag="VGY24_000863"
     CDS             8581..12147
                     /gene="rpoB"
                     /locus_tag="VGY24_000863"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ4283855.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDLDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12238..15879
                     /gene="rpoC"
                     /locus_tag="VGY24_000864"
     CDS             12238..15879
                     /gene="rpoC"
                     /locus_tag="VGY24_000864"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ4283856.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIADAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16031..16396
                     /locus_tag="VGY24_000865"
     CDS             16031..16396
                     /locus_tag="VGY24_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ4283857.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVTYLCKLNL"
     gene            16489..17427
                     /gene="tadA"
                     /locus_tag="VGY24_000866"
     CDS             16489..17427
                     /gene="tadA"
                     /locus_tag="VGY24_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ4283858.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQKA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLFEAGHLNPKQAKLEKII"
     gene            17411..>18070
                     /locus_tag="VGY24_000867"
     CDS             17411..>18070
                     /locus_tag="VGY24_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ4283859.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFVEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MA"
BASE COUNT         5274 a         3310 c         4019 g         5467 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tataattgaa aaacaaggtt tgtaaacctt gttttttgtt
      481 ttgatcttag gctgtagcta cgtagtacaa tctcttatat tactggtaag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttg aaaaaggttt gtcactacta ggtgcttttt tagctataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggtcgggt atcgggtaat ggagtgtggt tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgatagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggccaga gctttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggtacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcgct ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agttcaagac tttgtagagt tggagaattt ggttctttat
     1081 gaggaagaag tgcaggtaat tcgttgagcc agtcgtgaag atatcttagg tatgattgat
     1141 gaaggttcgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atattcgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgcaa aaatcaagtt aggccttgat actaagaata aacgatgaga tggttaccac
     1381 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa cattgatttc aagttaaaag aggactttcc
     1561 gtttacttgg aaaagcggat ttctatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1681 gatattggta taccgattgg tagtgatgta ccttattgct tgctttcagg ttgtgcccaa
     1741 gtcactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1801 ttggttaaac cagattttgg tatctcgact tgaacaattt ttctagatat taattgtaaa
     1861 accatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     1921 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     1981 tttattcaaa aataaaagag aaaacactgc aagctggtgc tgctaatgct ttgatgacag
     2041 gaagtggacc gactgttttt gctttgtgcc aaaccgaaaa acaaactata atagtttaaa
     2101 aggactttgc aaggaagttt ttaaggtgag aatgttgtaa tattttgttg ggattgcttc
     2161 tttggaagct tcatgttata attaactggt taatgaaaga ggagatttat ggggacttta
     2221 gaaaaaaaac ttgataactt agtaaatact attttattaa aagcagaaaa tcagcatgag
     2281 ttattatttg gagcttgtca aagtgacgtt aagcttacta atacgcaaga acatatttta
     2341 atgttactat ctcagcaacg tctcactaat actgatttgg ctaaggcatt aaatattagt
     2401 caggcggcag taactaaggc tatcaagagt ttggtcaaac aagacatgct agcaggaact
     2461 aaggatacgg ttgatgctag ggtgacttat tttgaattaa ccgagttagc taagcctatt
     2521 gcgtcagaac atacccatca tcatgatgaa accttaaatg tttacaaccg tttattacaa
     2581 aaattctccg cgaaagaatt agagattgta gataagtttg taacagtttt tgctgaggaa
     2641 ttagaagggt aatccatgag atacatatca gtgaaaaatc tctcctttca atatgaaagt
     2701 gagccagttt tagaagggat cacttatcat ttagatagtg gagaatttgt caccatgacc
     2761 ggtgaaaatg gtgctgcaaa gtcaacctta ataaaggcaa ccttaggaat tttacaacca
     2821 aaggctggac gagttactat tgctaaaaaa aataaagacg gtaaacaatt aagaattgct
     2881 tacttgccgc agcaagtagc tagctttaac gctggttttc catccaccgt ttacgagttt
     2941 gtcaaatcag gtcgctaccc acgtagtggt tggtttagac atttgaacaa acacgatgaa
     3001 gagcatgtgc aagcaagctt agaagcagtc ggcatgtggg aaaaccgtca taagagaatt
     3061 ggtagtttat caggtggtca aaaacaacgt gtggttattg cccgtatgtt tgcttctgac
     3121 cctgatattt ttgtgctaga cgagccaaca acgggaatgg atagcggtac tactgatacc
     3181 ttttatgaac tgatgcacca cagtgcacat caacatggga aatccgttct gatgattacc
     3241 catgacccag aagaagtgaa ggcttatgct gatcggaaca ttcatttagt cagaaaccaa
     3301 aaacttcctt ggcgttgttt caacattcat gaagttgaaa cagatgacga aaaaggaggt
     3361 catggtcatg cttgatattt tatcctatga tttcatgcaa cgggcggtaa tggcggtagt
     3421 tgccattagt atttttgctc cgattttagg tattttcctt attttacgtc gtcaaagttt
     3481 gatgagcgat acccttagtc atgtttcttt ggctggggta gcgcttgggg tagtctttgg
     3541 tatttcacca accatcacta ctattattgt tgtggtttta gctgctattt tgttagaata
     3601 cctgcgtgta gtttacaaac actacatgga gatttcaacg gcgattttga tgtcacttgg
     3661 cttggcccta tctctgatta ttatgagtaa gtcgcatagt tcatcaagca tgagtttaga
     3721 acaatacctt tttggatcga tcatcacgat tagtatggaa caagttgtcg ccttgtttgc
     3781 tattgctgcg attattttag tcttgaccgt tctcttcatt agaccgatgt acattctgac
     3841 ctttgatgaa gatactgctt ttgtagatgg tttgcccgtt cgcttgatgt ctgttctatt
     3901 caatatcgtc actggggttg ctattgcttt gaccattcca gcagcaggag cacttttggt
     3961 ttctaccatt atggtcttgc cagcaagtat cgcaatgaga ttgggtaaaa actttaaaac
     4021 agttatctta ctgggaattg tcatcggttt tagcggtatg ttatctggta ttttcttatc
     4081 ttatttcttt gaaacgccag ctagtgccac tattaccatg attttcatta gtattttcct
     4141 cttagttagt ctaggtggaa tgcttaaaaa acggttattt taataagaaa aatcctcaca
     4201 tgtttaacat gtgaggattt atttataaat gctttaaaaa gggggtaagg tcccaatttt
     4261 cttgattaat ggtgatgccc tcccaaaagc tatcattagt taaacgagga atcaaatgaa
     4321 ggtgaaaatg agtcccctca tccatcaact ccttgtcatt gctgacgcta gtaacgcctg
     4381 aaatgggaag gtgctgctct agcttagcaa ctagagcggc ttgtaagtcg atgagatcat
     4441 agcgcacttc ctttggtagt tgtgtcagac tagtgtaatg gtctttggaa atcagtaaaa
     4501 ggtgcccctt ttggatagga tcaatatcaa aaacaacctt gaaatattga ctttgagtca
     4561 gtagctgatt tgctttgagc tgatggcaaa agatacattc tgtcataggt acatctcctt
     4621 atgaaaaaac gagccagagc tcgtttaatc ggttatctag taagtgagaa cagcgtattt
     4681 tttcttaccg cggcgaataa cggttaattg atcatcaatt ttatcatcat tacttaattg
     4741 gtaatctaaa tcttgaacgc ggtctccgtt aatgtagatt gctccatttt gcacgtcttc
     4801 acgcgcctgg cgttttgatg gggaaatctt agcagctact aaaatctcga caatattatg
     4861 attgtctatt gattgaacgt gataatttgg tacattgctc aatccttgtt ttaattcatt
     4921 agcagaaaga tttttgatgt ttccagcaaa taactgctcg gtaatgttaa gagcttgttt
     4981 ataagcttct tcgccatgaa ccaaggtaac cacttcgcgt gccaaggttt tttgagcgag
     5041 acgctcgtga cgagcggcat taaattgagt ttcaatttct gcaatctcat ctagagataa
     5101 gaaggtgaag attttcaaga aacgcactgc atcatcatcc atgacattta accagaattg
     5161 gtacatctcg taaggagacg tcttatcggc atcaagccag acagcattac cttctgattt
     5221 accgaatttt ttcccggttg agtcagtgat gagtggtaca gtcatgacgt gcccagtttt
     5281 atcagccttt ttgcggagca attcagtacc agctgtcata ttaccccact ggtcagagcc
     5341 gccaatttgt aaggttacat tatgcttgtc attgagttcg tagaagtcgt agccctgcat
     5401 gatttggtaa gcaaactcag tgtaagaaat gcctgtttca atgcgttttt taacagagtc
     5461 tttactcatc atgtagttaa cggtgaagta tttaccgaca tcacggagga agtcaataaa
     5521 gctgatttgc gagaaccagt cgtagttgtt gacaagctct gctttattat caccattttc
     5581 aaaatcaagg aaagtagaca actgcccttt aatcttgtca ctccactcca aaactgtttc
     5641 tttggtttga aggctgcgct ctgcatcttt aaaggaagga tcaccaatta aacctgtggc
     5701 accacccaca agagcgtaag gtttgtgccc tgctagttgc aagcgacgag atgttaagat
     5761 agccactaga tggcccagat gaaggctgtc agcggttgga tcataaccgg tataatagga
     5821 tacttgccct tctgttaatg ctttgacaag ggcttgttca tcagtcgttt gaaagaccaa
     5881 gccacgagct ttgagttctt caaaaatatt cataggcttt ctccattttc attatagttt
     5941 cgaatcatta tatcaaaaag cgctactgct atgcaagctt tatcaaagtt tggtataatg
     6001 ataaatgagg tattttatgg tgaaatggaa cacgaaacaa aagcgtataa gtcatcaaag
     6061 attaggtctc ttggatttgg ggccggtgct attacgtacg ttgagactac tgtctaactt
     6121 tttttatatt gttatcttcc tttttggaat gatgggattt ggtatggcat ttgggtattt
     6181 ggctagtcag attgaatctg ttaaggtacc aagtaaagaa agtttagtca aacaagttga
     6241 atcattaacg atgatttcgc aaatgaacta ttctgataat agtttaattt ctactttaga
     6301 tacggattta cttcgaacac cagtagctaa tgatgcgatt tcagagaata tcaaaaaagc
     6361 tattgtatca acagaagacg aacattttca agaacataaa ggtgtcgtgc caaaagctgt
     6421 ttttcgggca acattggctt ctgtattggg atttggagaa gctagtggag gttcgacctt
     6481 aacacagcaa ttagtcaagc aacaagtttt gggagacgat cccacattta agcgcaagtc
     6541 taaggagatc gtttacgctc ttgccttaga gcgttatatg tccaaagaca atatcttatg
     6601 tgattatctt aatgtttcac cttttggtcg taacaacaag ggccaaaata ttgctggtgt
     6661 tgaagaagct gcgcgtggca tttttggcgt ttctgccaaa gatttaacgg tgccacaggc
     6721 agcatttttg gcggggcttc cgcagagtcc tattgtttac tctccttatt tgtcaacggg
     6781 acaactgaag tcagaaaagg acatggctta tggcatcaag cgtcagcaaa atgttctcta
     6841 taacatgtac cgtacaggtg ttctgtctaa aaaagaatac gaggactata aggcttatcc
     6901 gattcaaaag gattttattc aaccgggaag cgcaatagta aataatcacg attaccttta
     6961 ttacacggtg ttagcggatg ctaaaaaagc catgtatagc tatttgatta agcgagataa
     7021 ggtgtctagt cgtgacttga aaaatgacga gactaaggct gcttatgaag agagagcctt
     7081 aacagaattg caacagggtg gctataccat caccacaacc attaataagc ctatttacaa
     7141 tgcgatgcag acagcggcag ctcagtttgg tggcctgtta gatgatggca ctggtacagt
     7201 tcaaatggga aatgtcttga cagacaatgc gactggtgct gtgttaggtt ttgttggtgg
     7261 tagagattat gctctgaatc aaaataatca tgctttcaat acagttagat cgccaggttc
     7321 tagcattaaa ccgataatcg cttatggtcc tgctattgat caaggtttaa tggggagtgc
     7381 tagcgttttg tctaattacc caacaactta ctcgagtggc caaaaaatca tgcatgctga
     7441 tagtgaagga acagccatga tgccacttca agaggcccta aatacttctt ggaacatccc
     7501 agctttttgg acacagaaat tactgcgtga aaaaggggtc gatgtcgaaa attatatgac
     7561 aaaaatgggt tataagattg cagactactc gattgaaagt ttacctctag ggggcggtat
     7621 tgaagtctcg gttgctcaac aaaccaatgc ttaccaaatg ctttcaaaca atggcttata
     7681 tcaaaagcaa tatattgtag ataagattac tgctagcgat ggtacagtcg tttacaaaca
     7741 tgaaaataag ccaattcgta ttttttctgc agcaacagct acgattttac aagaattgtt
     7801 gagaggtccg attacttcag gcgctacgac tactttcaag aaccgtttgg cggctattaa
     7861 tccgtggctt gctaatgctg attggattgg taagaccgga acaactgaga attatacgga
     7921 tgtttggcta gtcctgtcta ctccaaaagt taccttaggc ggttgggcag gacatgatga
     7981 caatacctca ttagcgccat taacaggata taacaataat tctaattatc ttgcctattt
     8041 agctaatgcc attaatcagg ccgatcccaa tgttattgga gtagggcaac gcttcaactt
     8101 agatccagga gtcattaagg cgaatgtctt gaagtcaaca ggtttacaac caggaactgt
     8161 taatgtcaat ggacatactt tttctgttgg tggagaaatg accaccagtc tatggtccca
     8221 aaaaggaccg ggggctatga cttaccgatt tgctattggt ggcacggatg ccgattatca
     8281 aaaagcctgg gggaacttcg ggttcagaaa aaattaggtt gccatttatc gataaaaagg
     8341 gtataatagt aataactatt tgtcgtccaa tctagctgaa atattgtcca gttaggaaga
     8401 acagcagtta aatcacactg ataaagtcag atttagctgc tctttttgtg tctattttta
     8461 gaaaagatag ggtttgtaac ctatatttaa atattctaaa aattaacatt tagggcaaat
     8521 gataacttag ttgcgatttg ctgaaacagc aaagcttaaa tagaagaagg agctaaaaac
     8581 ttggcaggac atgaagttcg atacggaaaa caccgtacac gtcgtagctt ttcaagaatc
     8641 aaagaagttc ttgatttacc aaatttgatt gaaattcaaa ctgactcatt ccaagatttc
     8701 ctcgattcag gtttgaaaga agtatttgaa gatgtacttc ctatttcaaa ctttacggat
     8761 actatggaac ttgaatttgt tggttacgaa tttaaagaac ctaaatatac ccttgaagaa
     8821 gctcgtatcc acgatgcaag ttattctgca ccaatctttg ttaccttccg tttggtcaat
     8881 aaagaaactg gtgagattaa aactcaagaa gtcttctttg gtgatttccc aatcatgacg
     8941 gaaatgggta cctttattat caatggtggt gaacgtatca tcgtttctca gttggtgcgt
     9001 tctccaggtg tttatttcaa cgataaagtt gataaaaacg gtaaagttgg ttacggatca
     9061 acagtgattc ctaaccgtgg ggcttggttg gaattagaga ctgactcaaa agacattgcc
     9121 tacactcgta ttgaccgtac ccgtaagatt ccattcacaa ccttggttcg tgcccttggt
     9181 ttctcaggtg atgatgaaat tgttgatatc tttggtgaaa gcgaccttgt tcgtaatacc
     9241 attgaaaaag acattcacaa aaatccaagt gattctcgta cagacgaagc ccttaaagaa
     9301 atttacgagc gtcttcgtcc aggtgagcca aaaactgcag atagctctcg tagtcttttg
     9361 attgcgcgtt tctttgatgc acgtcgttac gatttggctg cggttggtcg ttacaaagtg
     9421 aacaaaaaac ttaatatcaa gactcgtctt ttgaaccaaa tcattgctga aaaccttgtg
     9481 gatgctgaaa ctggcgaaat tttggtagaa gctggaactg agatgactcg tagcgtcatc
     9541 gaatccatcg aagaacacct tgacggtgat ttgaacaagt ttgtttacac accaaatgat
     9601 tacgctgttg ttactgaacc agttgttctt caaaaattca aggttgtgtc accaattgat
     9661 cttgaccgcg tggtaactat tgttggtaat gccaatccag atgacaaggt acgtgctttg
     9721 acacctgctg atattttggc agaaatgtct tacttcttga accttgcaga aggtcttgga
     9781 aaagttgacg atattgacca cttaggtaac cgtcgtatcc gtgccgttgg tgaattgctt
     9841 gccaaccaat tccgcatcgg tcttgctcgt atggagcgta acgttcgtga gcgtatgtct
     9901 gttcaagaca acgatgtgtt aacaccacaa caaatcatca atatccgtcc tgtcacagca
     9961 gctgtcaaag aattcttcgg ttcgtctcag ttgtcacaat tcatggacca acacaaccca
    10021 ttgtcagagt tgtctcacaa acgtcgttta tctgccttag gacctggtgg tttgacacgt
    10081 gaccgtgctg gttatgaggt tcgtgacgtg cattacacgc actatggccg tatgtgtccg
    10141 attgaaacac ctgaaggacc aaacattggt ttgattaaca acttgtcttc atttggacat
    10201 cttaataaat atggtttcat ccaaacacct taccgtaagg ttgaccgtgc gactggtagg
    10261 gtaactaacg agattgtttg gttgactgcc gacgaagaag acgaatacac agttgcacag
    10321 gccaattcga aactaaacga agatggcact tttgctgaag aaatcgttat gggtcgtcac
    10381 caaggtaata accaagaatt ttctgcaagt gttgttgatt tcgttgacgt ttcccctaaa
    10441 caggtagttg ctgttgcgac ggcatgtatt cctttcttgg aaaacgatga ctccaaccgt
    10501 gccctcatgg gtgccaacat gcaacgtcag gctgtgccat tgattgatcc aaaagcacca
    10561 tatgttggta ctggtatgga atatcaagct gcccatgact caggcgctgc ggtgattgct
    10621 cagcacaatg gtaaagttgt cttttctgat gctgaaaaag tggaaatccg tcgtcaagat
    10681 ggctcacttg atgtttacca cattaccaaa ttccgtcgtt caaactcagg aacagcctat
    10741 aaccaacgca cccttgttaa agtaggagac attgttgaaa aaggtgattt catcgctgat
    10801 ggaccttcta tggaaaatgg tgaaatggct cttggacaaa acccagtcgt tgcttacatg
    10861 acttgggaag gttataactt tgaggatgcc gttatcatga gtgagcgcct tgtgaaagaa
    10921 gatgtctaca catctgttca cttggaagaa ttcgaatctg aaacgcgtga tacaaaactt
    10981 ggccctgaag aaatcactcg ggaaatccca aatgttggtg aagaagccct caaagacctt
    11041 gacgaaatgg gcattatccg tatcggtgct gaggtgaaag aaggcgacat cttagtaggt
    11101 aaagtcacac ctaaaggtga aaaagacctt tctgctgaag aacgtttact tcacgccatc
    11161 tttggggata aatcgcgtga agtgcgtgat acctcacttc gtgtccctca tggtggtgat
    11221 ggtattgttc gtgatgtgaa aatctttaca cgcgctaacg gcgatgaatt gcaatcaggt
    11281 gttaatatgc ttgtgcgtgt ttacatcgct caaaaacgta aaatcaaggt cggagataaa
    11341 atggccggtc gtcacggaaa caagggtgtc gtttcacgta ttgtaccagt tgaagacatg
    11401 ccataccttc cagacggaac accagttgac atcatgttga accctctcgg ggtgccatca
    11461 cggatgaata ttggtcaggt tatggaactt caccttggta tggctgctcg taatcttggc
    11521 attcacattg caacacctgt ctttgacggg gcttcatcag aagacctttg ggacactgtt
    11581 cgtgaagctg gcatggatag cgatgctaag acggtccttt atgatggtcg caccggtgaa
    11641 ccatttgaca accgtgtatc cgttggtgtc atgtatatga tcaaacttca ccacatggtt
    11701 gatgataaac ttcatgcccg ttctgtagga ccatactcac ttgttaccca acagccactt
    11761 ggtggtaaag ctcaatttgg tggacaacgt tttggtgaga tggaggtttg ggcccttgaa
    11821 gcttatggtg catcaaatgt tcttcaagaa atcttgacct acaagtcaga tgacgtcaca
    11881 ggacgtttga aagcctatga agcgattact aaaggtaaac caattccaaa accaggtgta
    11941 ccagaatcct tccgtgttct tgtaaaagaa ttgcaatcgc ttggtcttga tatgcgtgtg
    12001 cttgacgagg atgataatga agtggaactt cgtgatcttg atgaaggtga agacgatgat
    12061 attatgcatg ttgacgatct cgagaaggca cgtgaaaaac aagctcaaga aacccaagaa
    12121 gtttctgaaa caactgacga aaaataagca atcaattctt attaaataat tatttactgg
    12181 tctggggcaa aggccccagg aactggtaaa gtcatcaaag gcagaaaggt aaaactagtg
    12241 gttgacgtaa atcgttttaa aagtatgcaa atcacattag cctcaccaag taaggtccgt
    12301 tcatggtctt atggtgaagt taaaaaacct gaaacaatca actaccgtac attaaaacca
    12361 gaacgtgaag gactctttga cgaagtcatc tttggtccaa caaaagactg ggaatgtgcg
    12421 tgtggtaagt acaaacgtat ccgttataaa gggatcgttt gtgaccgctg tggggttgag
    12481 gtaactcgtg ctaaagtacg tcgtgaacgt atgggtcaca ttgagttaaa agctcctgta
    12541 tcacatatct ggtatttcaa aggaattcca tctcgtatgg gattgactct tgatatgagt
    12601 cctcgtgccc ttgaagaagt gatctatttt gcggcttatg tggtcattga tccaaaagat
    12661 acaccgcttg aaccaaaatc attattaaca gaacgtgaat accgtgaaaa actacaagag
    12721 tatggtcatg ggtcatttgt cgctaagatg ggtgctgaag ccattcaaga tctcttgaaa
    12781 cgtgtggact tggcagctga aatcgctgaa ttaaaagaag agttgaaatc tgcttctggt
    12841 caaaaacgga ttaaagcagt tcgtcgttta gacgtccttg atgcctttaa caaatctgga
    12901 aataaaccag aatggatggt tcttaacatc ctgccggtta ttccaccaga tcttcgtccg
    12961 atggttcaat tggatggtgg ccgttttgcg gcatcagact tgaatgactt gtaccgtcgt
    13021 gtgattaacc gtaataaccg tttggcacgt ttgttagaac ttaatgcccc tggcatcatt
    13081 gttcaaaatg aaaaacgaat gcttcaagaa gccgttgatg ctttgattga taatggtcgt
    13141 cgtggtcgtc caatcactgg accaggaagt cgtccattga aatcattgag ccacatgctt
    13201 aaaggtaaac aagggcgttt ccgtcaaaac ttgcttggta aacgtgtaga cttctctgga
    13261 cgttccgtta tcgctgttgg tccaaccctt aaaatgtatc aatgtggtgt cccacgtgaa
    13321 atggctatcg agctttttaa accatttgta atgcgcgaaa ttgttgccaa agaatatgct
    13381 ggtaacgtta aagccgctaa acgtatggtt gaacgtggcg acgaacgcat ctgggatatc
    13441 ttagaagaag ttatcaaaga acacccagtc ctacttaacc gcgcaccgac ccttcacaga
    13501 cttggtattc aggcttttga accggttctt attgacggta aggcacttcg tcttcaccca
    13561 cttgtgtgtg aggcctacaa tgccgacttc gatggagacc aaatggccat ccacgtgcca
    13621 ctttcagaag aagcacaagc tgaagctcgt cttttgatgc ttgctgcaga gcacatcttg
    13681 aaccctaaag atggtaaacc agttgttact ccatctcagg atatggttct tggtaactac
    13741 tatcttacga tggaagatgc tggtcgtgaa ggcgaaggta tgattttcaa ggataaagac
    13801 gaagctgtga tggcatatcg taatggttat gctcatcttc atagtcgtgt gggtatcgct
    13861 gttgacagca tgccaaacaa accttggaaa gacagtcaaa gacataaaat catggtgaca
    13921 actgttggta agattctctt taacgatatc atgccagagg accttcctta cctccaagag
    13981 ccaaacaatg ccaacttgac agaaggaaca cctgataaat acttccttga acctggtcaa
    14041 gacatccaag aagtgattga tcgcttagac atcaatgtgc catttaagaa gaaaaacctc
    14101 ggtaacatca ttgcggaaac cttcaaacgt ttccgtacaa cagaaacatc agccttcctt
    14161 gaccgcttga aagaccttgg ttactaccat tcaacccttg ccggtttgac agtgggtatc
    14221 gctgacatcc cagttatcga taacaaagca gaaatcattg atgctgctca ccatcgtgtt
    14281 gaagaaatta acaaagcctt ccgtcgtggt ttgatgacag atgatgaccg ttatgttgcc
    14341 gttacaacaa catggcgtga agctaaagaa gcccttgaaa aacgtctaat tgaaacacaa
    14401 gatcctaaga acccaatcgt tatgatgatg gactcaggag ctcgtggtaa catctctaac
    14461 ttctcacagc ttgctggtat gcgtggtttg atggctgctc ctaacggacg catcatggaa
    14521 cttcctatct tgtcaaactt ccgtgaaggt ttgagcgttt tggaaatgtt cttctcaacc
    14581 cacggtgcac gtaaagggat gaccgatacg gcccttaaaa cagccgactc aggttatctt
    14641 actcgtcgtt tggttgacgt tgcccaagat gttatcattc gtgaggacga ttgtggcact
    14701 gatcgtggtc ttcttatccg tgctattaca gatggtaaag aagttaccga aacgcttgaa
    14761 gagcgtcttc aaggtcgtta cacacgtaaa tcagtcaaac accctgaaac tggtgaagtc
    14821 ttgattggtg ctgaccaatt aatcactgaa gacatggctc gtaagattgc tgatgcaggt
    14881 gttgaagaag tgaccattcg ttctgtcttt acctgtgcga ctcgtcatgg tgtctgccgt
    14941 cactgttatg gtatcaactt ggcaactggt gatgctgttg aagtgggtga agcagttggt
    15001 actattgctg cccaatctat cggtgagcct ggtactcagc ttaccatgcg taccttccac
    15061 acgggtggtg tagcctcaaa taccgatatc acccagggtc ttcctcgtat tcaagagatc
    15121 tttgaagcac gtaatcctaa aggggaagcg gtcattactg aagtgaaagg gaatgtcgtc
    15181 gagattgaag aagatgcgtc aactcgtacc aagaaagtct acgttcaagg aaaaactggc
    15241 atgggcgaat atgtcgtacc atttacagca cgtatgaaag ttgaagttgg cgacgaagtt
    15301 aatcgcggag ctgcccttac agaagggtca attcaaccga aacgtctcct tgaagtgcgt
    15361 gataccttgt cagttgaaac gtaccttctt gcagaagtac aaaaagttta ccgtagccaa
    15421 ggggtagaaa tcggagacaa acacgttgag gtaatggttc gccaaatgct tcgtaaagtt
    15481 cgtgtcatgg atccaggtga tacagacctc cttccaggta cacttatgga tatttctgat
    15541 ttcacagatg ctaacaaaga tattgttatc tctggtggta ttcctgcgac atctcgtcct
    15601 gttcttatgg gtattactaa ggcttccctt gaaactaatt ccttcttatc agctgcatcc
    15661 ttccaagaaa caactcgtgt tcttacagat gctgctatcc gtggtaagaa agaccactta
    15721 cttggtctta aagaaaatgt tatcattggt aaaatcattc cagctggtac tggtatggcg
    15781 cgctaccgta acattgaacc acaagcgatg aatgagattg aagtgattga tcatacagaa
    15841 gtctcagcag aagccgtttt tacagcagaa gcagaataat ctcatgaaag agtccttagg
    15901 ggctcttttt tgtctgagtt tgttggcagt aaaaagcatt gacaatcagt cttagtccca
    15961 atgagcagat gtttttcaaa atacttatga tttagctata ataatgcata agaggaatag
    16021 gtaatgtgtt atgtatcaag taattaaaat gtatggtgat tgggagcctt ggtggttcat
    16081 cgatggatgg caagacgata ttatagatga gcaacaattt agtgactggc aagaagcgct
    16141 tgattatttt aatcaagaat ggcagcgcat gaaagctatt tttcctagtt atcatagcca
    16201 aaagaatttg ttagctactt tttgggaaaa agaagataaa agatggtgcg aggactgtga
    16261 cgaagatttg cagcagtttc attctctttt actccttaaa aataaagata ttgtaccaag
    16321 caataattat atccctgaat ttgaacaacg aaatgattca ccacaggtaa cttatctttg
    16381 caagttaaac ctttaagtgt ttgttaaaaa caaaataagt taaaagaaaa tatcacttct
    16441 tctgatattt tctttttttg tttacgaata aatgagtaag gaggaattat ggtacaagca
    16501 ttagcaaaag ctattctagc aaaagctgaa caggttcagg cacaagatat ttatattttg
    16561 ccaagagcag atcaatatga tcttttttta cgaataggag atgaaaggag attagttgat
    16621 gtttatcaga gcgatcggat ggctcctctt attagtcact ttaaatttgt tgcaggaatg
    16681 atagttggtg aaaagagacg ttgtcaggtg ggttcatgtg attataagct tagtaaagat
    16741 aagcagttat ctttgcgctt atctagcgtg ggtgattatc gcgggcaaga aagcttagtg
    16801 attcgtctgc ttcatcatca aaataaaagt gtacattatt ggtttgatgg attgacaaaa
    16861 gtagccaatc aagttggcgg tagagggttg tatttatttg caggaccagt tgggtctggg
    16921 aagacaacct tgatgtacca gctgatttcg aattatcatc aaaaagcaca ggttattagt
    16981 atagaagatc ctgtagaaat taaaaatcac cagattttac aattacaagt gaatgatgat
    17041 attggtatga cttatgacaa tttgatcaaa ctgtctttac gccatcgacc agatatttta
    17101 gttattggtg agattcgaga tagtcaaaca gcaagagccg ttattagggc tagtctaaca
    17161 ggtgccatgg ttttttcaac ggttcacgct aaaagtatct cgggtgttta tgcaagattg
    17221 ttagaacttg gtgtaacgaa agcagaactg tctaattgct tagcattaat tgcttaccaa
    17281 aggttactta atggaggagc attgattgac tctactcaaa acgaatttga atattattcc
    17341 tcatcgaact ggaatcaaca aattgatcag ctttttgagg caggacatct caatcccaag
    17401 caagctaagc ttgaaaaaat tatctagtaa acatcagcat aaattcattc aattactagc
    17461 taatctttta tctacaggat ttagcttcgt agaggttatt gcttttttaa aaagaagcca
    17521 gttgcttcag ctagattatg ttcttaaaat ggaagagtct ttattaaaag gacaaggctt
    17581 agcagatatg ttgtcgggtt taggtttttc agatgctatt cttactcaaa ttagtttagc
    17641 tgatagacac ggtaatattg aaacaacatt agtagcgatt caacattact tgaaccaaat
    17701 ggcaaggatc agacgaaaaa ctgttgaagt tatcacctat cctcttattc ttttactttt
    17761 tctttttgtg atgatgctag ggttgagacg ctatttagtc cctcaattag aaactcaaaa
    17821 tcagataact tactttctta accattttcc tgccttcttt ataggttttt gctcaggtct
    17881 tattttgttg ttcgggatgg tatggctacg atggcgatct caaagccgct tgaaactcta
    17941 tagtcggcta agccgttatc cttttttagg taaactgtta aaacaatact taactagtta
    18001 ttatgctaga gaatggggta cgttaatagg tcaaggtctt gatttgatga ctattttaga
    18061 cattatggcc
//
LOCUS       DAUCWU010000027        17380 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUCWU010000027 DAUCWU010000000
VERSION     DAUCWU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.027"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(478..1923)
                     /locus_tag="VGY24_000868"
     CDS             complement(478..1923)
                     /locus_tag="VGY24_000868"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ4283860.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(2105..2815)
                     /locus_tag="VGY24_000869"
     CDS             complement(2105..2815)
                     /locus_tag="VGY24_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ4283861.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2946..3443)
                     /locus_tag="VGY24_000870"
     CDS             complement(2946..3443)
                     /locus_tag="VGY24_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ4283862.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVREDMAKIRASSLISDDVVINGAVYDVDTGKM
                     TQVME"
     gene            complement(3632..4993)
                     /gene="radA"
                     /locus_tag="VGY24_000871"
     CDS             complement(3632..4993)
                     /gene="radA"
                     /locus_tag="VGY24_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ4283863.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLNA
                     VFS"
     gene            complement(5074..5520)
                     /locus_tag="VGY24_000872"
     CDS             complement(5074..5520)
                     /locus_tag="VGY24_000872"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ4283864.1"
                     /translation="MTKIRGFELISSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5630..6397)
                     /locus_tag="VGY24_000873"
     CDS             complement(5630..6397)
                     /locus_tag="VGY24_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ4283865.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWEKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6515..8299)
                     /locus_tag="VGY24_000874"
     CDS             complement(6515..8299)
                     /locus_tag="VGY24_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283866.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLIWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQGDTVTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNAFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8302..10008)
                     /locus_tag="VGY24_000875"
     CDS             complement(8302..10008)
                     /locus_tag="VGY24_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283867.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     NSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(10001..10450)
                     /locus_tag="VGY24_000876"
     CDS             complement(10001..10450)
                     /locus_tag="VGY24_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4283868.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10746..11762
                     /locus_tag="VGY24_000877"
     CDS             10746..11762
                     /locus_tag="VGY24_000877"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ4283869.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     HNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11795..12694
                     /gene="galU"
                     /locus_tag="VGY24_000878"
     CDS             11795..12694
                     /gene="galU"
                     /locus_tag="VGY24_000878"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ4283870.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12793..13464)
                     /locus_tag="VGY24_000879"
     CDS             complement(12793..13464)
                     /locus_tag="VGY24_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ4283871.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAIQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVSVL
                     VLGFL"
     gene            14152..15660
                     /locus_tag="VGY24_000880"
     CDS             14152..15660
                     /locus_tag="VGY24_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4283872.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNKLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(16009..17358)
                     /locus_tag="VGY24_000881"
     CDS             complement(16009..17358)
                     /locus_tag="VGY24_000881"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ4283873.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
BASE COUNT         5282 a         3709 c         3122 g         5267 t
ORIGIN      
        1 gatagaaagg aaaaaatata aatttttgtc atataataga caataattga taataataaa
       61 aacatatatt cgctattagc catttttttc tcctttccaa ctttattatt ttagcatatt
      121 ttatgacaat gattacaata ttatttgatt acaattgatt agctaagtga gtactaaact
      181 agtctctttc agtatatacc aaaaaaatat tgtacactaa ttgattcata aaaaccattt
      241 ctttaaatca cactaatcaa aacctcaccg tttgctgtca ttatcacgta agcatctcac
      301 cacctctctt tcaaatccaa tctccaaaat agaaataaac tatactattt tttactttac
      361 caaggtagca agctcagata acgataaaaa agactgaaga acttaatctt caatctttcg
      421 ttgtcgtgac tttttaactc cttaaagcgt ttaaaaataa aggtgaacga tacagcctta
      481 tagcatgttt ttgatgtgct caatggattt atcacgtccc aataaataaa tcgtatttgg
      541 taattctggg ccatgcattt caccagatac tgcaatacga ataggcataa agaggttttt
      601 ccctttgatt cctgtttctt tttgaacagc cttaatttgt gggaaaatat tttctggttt
      661 gaagtcttca tctgacatag cttccaactt agccttgaaa gcttgcaaga ctgtcgaaac
      721 tgtttcacct gccatcactt ctttttcggc ttctgttaat tcagggaaat cagaaaagaa
      781 aaggtcagtc aatggaataa tctcatctgc agattttaat tgaggtttgt ataattctac
      841 tagcttctca gctttttctg tcaaacggcc tgcctcttca aggaatggtt tgcagagggc
      901 ataaactgtt tcaaaatccg catgtttaag atactcattg ctcatccaat ccattttctt
      961 ctggtcaaag gcagctggcg acttgctaag gcgattttca tcaaaaaggg caatcagttg
     1021 ctcacgagag aagatttcct cttctccgcc aggattccag ccaagaaggg cgatgaagtt
     1081 aaagacagct tcaggcatat aacctttttt gcggtaatct tcaatgaatt ggagggtatt
     1141 ggtgtcgcgt tttgataatt ttttacccgt ttcagaattg atgataagag tcatatgacc
     1201 aaactctggc gcttcccagc caagagcttc atagaccatc aattgttttg gcgtattggc
     1261 aatatggtca tcgccacgga taacgtgaga aatttgcata tcatgatcat caacaacaac
     1321 ggcaaagtta taagttggat agccatcttt cttttgaata acccagtcac caccgatgtt
     1381 accaccttca aactcgatat cgcctttaac catgtcagtc catttgtaaa taccggactc
     1441 attaacagca agacggactg ttggaacgat acctgctgct tcacgctcag caatgtactt
     1501 agctttttcg tcagctgaca taccaataaa ttcattgata tagcgaggcg tttcgccagc
     1561 tgcttcttgg cgttcacgct ctgctgccaa ttcttcctca gtcacgtaag acttgtaggc
     1621 ttttccttca gctaacaatt gatcaatata ttgttgataa agtgctagac gttcagactg
     1681 gcggtaattt tcatgcgttt ctgggctttc atcccaatcc attcctaacc attttaggtt
     1741 ttcaagttga gaacgttcac catcttcaac atgacgttta cggtcagtat cttcaatacg
     1801 aatgataaaa gtacctccgt gacgacgggc ataaaggtaa ttaaataggg ctgtgcgggc
     1861 attcccgata tgtaaaagcc ctgttggact tggtgcataa cgcacacgaa ttggtttaga
     1921 catgcttctt ctcctgttac atatatcact cagaaacctg tggcacgaaa aacaagcaac
     1981 aaatgatgat ttgagcttgc caagaccttt tcagacttct tctaaaatct ttcttattat
     2041 agcatatttt acttgcttag gcgaaaaaaa tcagccctaa aaagctgatt ttatgttatt
     2101 ttgactaatt tgaactagtt ggtagataac gtttaagagt cccagcaaat tgctcaaggt
     2161 taagactttg gatataaatc tcaccatgac ctctaaaggt attaacaact ccttcacctg
     2221 taccaattga ctgcataaag ccattttcta aatgaatatc ataatcaagt tctctgctcc
     2281 atgctacaac atgggcatta tcaatagtca ttgtgccacc atcaagtgtg attttcttga
     2341 tagatccaaa cgagttagca agtaaggtac caaggccttc agttgtcatg acaaaaagtc
     2401 cgccttgacc accaaaaagt gcttttccaa tattttgacg ttccattttg tactgtgctg
     2461 aaccatctag ggctagaaag gcaccatcat ttagacgata ttgtttttca cctagttcta
     2521 aagcaacaat ctgtcctggt gtattgggtg ctaatgctaa tttaccatct ccatttgaca
     2581 tagcttgtgt aatgaacatt gattcaccag aaaccatgga acggccgatg gctccaacta
     2641 attttcctaa accagatccc ttaccattca atttggtatt caaggttaca ttctcagtat
     2701 gataaaccat acttccttgt tgtaagtaaa ctgatccccc atgttccaga tcaatctcaa
     2761 ctagtggaaa ttgcatgttt tgatcaatcg taaatctcat tctattgtcg accatcttac
     2821 tctccttgtt tgtatgaata acttgataca attattataa cgcttcccta tttctttgtc
     2881 taatattttc aataattttt aaaaataaaa aagagctgaa ttcagctcta caacttggct
     2941 actaactatt ccattacttg tgtcatcttt ccagtatcca catcgtagac ggcaccgtta
     3001 ataacgacat catcagagat aagagaggag gctcttatct tagccatatc ctcgcggaca
     3061 ctgtcttcca catcttggaa gggaaggaaa tcttgccctg aaacatccac acctaagtgc
     3121 tcatgaatat gcttggcaaa gccttcatta gtaaaagtct gcgcgccaca atctgtatgg
     3181 tggagaacca caatctctct cgttcccatt tgctgctgag aaataaccag cgaccgaatc
     3241 atgtcttcgg tgacacgtcc gccagcattc cttaaaatat gagcatctcc tagagccagc
     3301 cccagagctt gggccacatg tagccgtgaa tccatacaag tcacaatcgc tactttagtc
     3361 ttgggtttta agggcaaatg agctgtccca tgcaaggcca cataagcctg attggcagcc
     3421 ataaaatgtt caaaataaga catcaataaa ctccttttga gaaacagcag agctattttt
     3481 gctgttagtt tgtcttatct taacatcttt tatggaaaat agctggatta acctcttaga
     3541 cggtcggtat cgttcgaaaa gttaaaaaaa caaatgattg ggatactttc ttgacaaatt
     3601 tcctcctcat ttgctcttag tttctgatcc tctagctaaa aactgcattt aaaacctgac
     3661 caaccgtagt cactccgaca acctcaatac cttgagggat atcaatgcct tgcaaagcat
     3721 ttttaggagc atagaccttg gtaaatccaa gctttgctgc ttcattaatc cgttgctcaa
     3781 tacgggtcac tcgccgaatc tcacctgtta acccgatctc gcctaagaaa gcttcttgag
     3841 ggctggttgg tttttcctta taactggatg cgatagccac agcgactgcc aaatcaatgg
     3901 caggttcgtc tagtttgaca ccaccagccg acttgaggta ggcatcttga ttttgtaaga
     3961 gtaaaccaca gcgcttttct aaaactgcca ttattaggct tacgcgattg aaatcaagtc
     4021 ccgtagtggt tcgtctggca ttgccaaaaa cggttggtgt cactaaagat tgcacctctg
     4081 ctaaaatagg acgacttcct tccatcgtta caacgacagc cgatcctgtt gccccatcta
     4141 agcgttcctc taaaaagact tgactaggat ttaagacctc taccaagccg ccagactgca
     4201 tttcaaagat gccaatctca ttagtggagc caaagcggtt tttcacagcc cgcagaatgc
     4261 gaaaggtgtg atgcctttcc ccttcaaagt agagaacggt atccaccata tgttctaaca
     4321 tacgaggccc agccaaggtt ccttccttgg tgacatgacc cacaataaag gttgcaatgt
     4381 tgttcgtctt tgcaagctgc atcaattctg cagtgacttc tctcacctga ctgactgatc
     4441 cttgaacacc tgtaatatca gggctcatga tagtctgaat ggagtcaata attaaaaaat
     4501 ctggtttaat attttcaatt tctgtgcgaa tagcttgcat attggtttcg gcatagaggt
     4561 aaaattcatt gtcaatatca cccagacgct cactccgcag tttaatctgc tctgctgatt
     4621 cttccccaga cacatagaga accgttccct tattagcgag ctgcgtggat acctgcaaga
     4681 gtaaggtgga ttttccaata cctggatcac caccaattaa aatcaagctt ccaggaacca
     4741 caccccctcc aagaactcta ttaaattcag acatgtcagt ctgtgttctg tgataactaa
     4801 tattatccac atcttttagt ttaaccggtc tggacttttc tccagctaga ctaacacggg
     4861 catttttgac ttctttgacc tctacttctt ccacaaaaga agaccaggcc gagcaatttg
     4921 gacagcgccc taaatacttt ggcgactggt agccacactc ctgacagata aaggttgctt
     4981 ttttctttgc catagtaatc tccctcccct agacaagaca aacctactct taaagaatag
     5041 gtctgctgtc ataagcattt ttgttaaatc gctttactgc cctgtcgaac caaaaccacc
     5101 tgttcgttca cctctggctt ggtcaccatc agcaattaaa aaaggcataa aaacaccctg
     5161 aacgatcctc tcacccacag ctaatgtcac agggtgatct gtaatgtttt gcatttgtgc
     5221 aaaaatatgt ccttcattag cctcattgcc ataataatct gcatcaatca cacctactga
     5281 attaatcagg ataatccctt tttttcgtgg atttgacgaa cgatcataga ggtaaagtac
     5341 ctcaccatct tgcatatagg ccttaacccc tgttggtacc aacttaatct cccctggggc
     5401 aatggttaca gcctcagcaa cagacaggtc ataaccagct gcatgagtcg tctcacgttt
     5461 tggtagtaaa tcaggattag taaagcttga aataagctcg aaaccacgaa tttttgtcat
     5521 tatgataggt atatcctttc aagtcaatca tatcctctta ttatagacta ttcgtaaaag
     5581 aatagcaaaa aagcggtcaa ctttaaacgg accactttta atcatgccat cacatctcat
     5641 ccccacgata actaaagcgg atatgggtga aatcattatc caaatccttg tctgccataa
     5701 aagctttggc atcttttttc acttgaacca agtcaatacc ttgcattttg gcagcataga
     5761 cacagccaca atagcattgg cggtaaatat catactcctc acacatttca acggatctgc
     5821 gataaccgtt gtttttcttg aaatcacttg gcaggtattt ggtggtgtaa accttttgca
     5881 catcgatgcc cacatcatta atggtttgcg aatttttatg aggactgatg gttagggcac
     5941 tggcaaagta gtcaaaacca agttccactg ctttttgagc ggtcttgtct agtcggtaat
     6001 caaaacagac acggcaacgg tccccacctt ctggttcctc ttctaagcct cgcacttgtc
     6061 gaacgtactc attggggaca tagtccgctt cgagaaactg cacggtattg cctgttttag
     6121 cgttgaactc ggagacaaac tgttgtgtca cataggctct gcgatggtat tcgtccttag
     6181 ggtgaatatt agaattggca aagtaaaccg taatgtcagc aaactgcgtc aggtattcta
     6241 gggtgtaagt ggagcatggg gcgcagcaga catgcattaa aatactgggg cgcacggatt
     6301 ccttctccca aaccttggcc atctgttgca tgaccctgtc ataatttatc ttttgattgg
     6361 gattcatgtt agcgagaatc tcttgtaaat caatcattgt ggctactttc taattgtttt
     6421 ttctcttgct tgctattata ccatatcagg aaagaaagct tgactgcttt attgttcacc
     6481 aaaaaactcc gctaagacgc ggagctgata ccatctattc aaagacaaat tgattgtgat
     6541 acaattcggc ataaaagcct ttttggtgca atagctcatg atgatttcct tgctcaatga
     6601 ccttgccatc tttcaacaca ataatgtgat ctgcatttaa aatggttttg aggcggtgag
     6661 caatgacaaa gctggttcga cctgccacga tagcttccat ggcccgttga attttacttt
     6721 cggtaactgt atcaacattt gaagtggcct catccaaaat caacacttca gggtcagtca
     6781 gtagcgtcct agcaatagaa atcaactgct tttgacctgt tgaaaaggca ttgtcatcat
     6841 ctgagacata ggtattgtac ccttttggta aggacatgat aaagtcatga atatgggtcg
     6901 cacgcgcagc agtttcaacc atgtcttgac taatggtctg atcaccaaaa cgaatattat
     6961 ccgtaatggt tcctgaaaaa agaactgact cttgcaacac aatccccacc ttttgacgaa
     7021 gactatccaa atcgtagtca cgaatatcac ggccatcaaa ggtaatcgaa cctgcatcca
     7081 catcgtagaa acggttaatc aagttcataa tagtggtctt tccagaacct gtcggtccaa
     7141 ccacggcaat cattttgccc ttgggtgcaa cgattgacac atctgataaa actttttgcc
     7201 caggaagata cccaaaatcg acgtggttaa tcgccactgc ttcttttaag ctggtgaacg
     7261 ctggtgcatt ttgtggacga acttcttcgg tttcatcaaa catttcttga atacggtgag
     7321 caccggtaaa ggccagctgc aattctcccc aactagacgc gatttgcatc atgggttggt
     7381 aatattgctg ggaatattgt acaaaagtaa ccactaaacc aagcgctgcc gctgctggca
     7441 tagatttgtc actgaggaca attgttgaac cgacaaaaat cacgatagcc gtgttaataa
     7501 ggctcattcc attcatgact ggaaataatt gtcctgagaa cagacggcgt ttgaaggtgg
     7561 cttgtcgaac cctttcatta tgctttaaaa aggctgtcac cgtatctcct tggacacctt
     7621 gtacaataat agccttttgt cctgaaatgg tttcatccat aaaagcattt aaagcactga
     7681 cttcttgctg ttggatattg gtgtattttc ttgccaaacg gatgttaatc actaaaaaaa
     7741 tgagagcaac tggggttgat gcgatggtta acaaagctaa acggctatct tgcctaaaca
     7801 tcatccagat caggccgatg taaagggcaa tattagtcac cacttgaacc aaggattggt
     7861 tcagcgagtt ttgaatatta tccaaatcac tcgtgaaacg agaaagaatc tccccatctt
     7921 tatggcggtc aaaaaaggcg acggttaaac gttctaattt accaaacaag cccttgcgca
     7981 tgcggttggt cgaatgtgag acaacacgtg tgagtaagaa actatatatt agattagcta
     8041 aaacagtaaa gaaataagtc atcaaaagct taaacatcac ggcattaaaa gctgataaat
     8101 cagggctaaa atgtgtctga ccactcatct tagcaacgta ataagcctga ccgatttttc
     8161 ccaactcagt caaggactcc cctaagaaga caggagcttt tacttgtaaa taagttgcta
     8221 agataacggc aacagcaatg acagtaaacg agaaacgata gcgtttaaaa taaaaccaga
     8281 aaaaacgtgc tgttttcatc actactcctc ccttcctttt tgtgtctcat aaatttcacg
     8341 ataaatcgca ttattagcca ccaactctgc gtgtgttcct tctccaatta actgtccttg
     8401 gtcaaggact aagatcttat ctgccttaac taccgatgaa attttttgag caacaataac
     8461 tgtagttgta ccttctaatt tatgacttaa ggcttcttga acgcgttttt cagattttgc
     8521 atcaagagct gaagtggaat cgtctaatat aaggattttg ggatgattaa tgacaccacg
     8581 tgcaatagac agacgttgct tttgtccgcc agacaagtta ctacctcgct cttcaacttg
     8641 gctctcataa cggctattca tgcgatcaat aaattctttt gcttgcgcga tttgagcggc
     8701 tttttgcata gcatcaatat cagcttttgc tgagccttgg cgaaggttat cagctatcgt
     8761 tcctgaaaag agaatagcct tttgcaacac gatggaaacc gattggcgca gggttgtttg
     8821 acttagggtt ttaatcggct ttcctcctaa caaaatttgg ccatcttgag gatcaaataa
     8881 gcgagggatg agctgtacaa gcgttgattt cccagatcct gttgcaccaa cgatgccgac
     8941 catttgccca ggttcaatag caaaagaaat gtgcttcaag gttggctcat catcattagg
     9001 atatgtaaaa ctaacatcgt caaatacaat gcttcctgag atggtttctt ccttttcatt
     9061 ttcaaatgtc atggcaggtt ctgtagacaa tatttgccta attctagcca tagaaacaaa
     9121 agcacgacta acttgcatcc ccatggaacc aaccacaata attgaaaaca taatttgcat
     9181 catataagtc atgaaagagg caatattccc aatcactgtt ggatctgttt cgaccatggt
     9241 cgaaacaaca tttatagaaa cataaactgc caaataagac accaacatta aagccggctg
     9301 cattagtgaa aaaccataac caatagataa atttaacgcc aaaagatcat tggacgtttc
     9361 tttaaattta gtgtattgtt gttgttcttg tacaaatgat ttaaccacgc gaacgccacg
     9421 gagattttcc ttagcgatgc gattaatctt atccattaac cgttgaaatt taccaaaacg
     9481 aggacccatt tgacgcatca ccaaacccat gatgagggca attaagataa ccattacaac
     9541 aatgacccac cataattgag ggaatgtctg aactgccata ataaacgcgc caataaaaag
     9601 gatcggcaaa cgaaagagaa tttgaaacat catcatgact aaactttgga tttgattgat
     9661 atcattagtt agcctaacca caagattacc agcattaaaa gcttcaatat tggcatatga
     9721 aaagtcttgg atttttcgaa aggtcttctc cctcatatca gcagatacac cttgagctat
     9781 tttagcagct aaaaccgtat taatcgctcc agacattaac cctaaaaggg caataataag
     9841 taatggtttc ccaacctcat aaattttctc ttgattattt tgaataacag cgcttagcac
     9901 agatttaaga taatgtggtt gcaagagact acttgcaaca accccaataa ccataaaaaa
     9961 tgagcctaaa gcataccatt tataacgtaa gattgcgtct ttaagcatcg ttgtctcctt
    10021 tataagttag catattttgc ttgagctgtt caattacttt tttcgctgtc agcaactctt
    10081 cttttgtaat ttcttttaaa aaatagtgct cgatatcctt acgacactcc cgcaacaaag
    10141 gcaatttatc tctaccaacc tgcgctaaca ctacttgctt ataacgctta tccacttggg
    10201 aaggcatcac attgataaac ccatttttga ccatacgttt cactaaatga ctagcaactg
    10261 actttgagat acgaagttgt ttttcaatat ctttgacaaa tatttcttga ttttggtgtt
    10321 tagctaaaaa aacaagaaca taaccttgag gacctgctag atgctctaca tcatattttt
    10381 ttgcaatctc atcactaatt tgttcgattt gatgtatcaa ttcacgtaaa tcacctatca
    10441 cttgtgacat ctcttccctc cttcttttcc ttaatagttt ctatgataag tattttagtt
    10501 ctctagataa ctattgtcaa ttaaaatatc tttattttta aaaataagtt tccgaactta
    10561 aataactaaa aagaacaggg caaagccatg acaacagctc acctgatgaa gcttattaaa
    10621 caacattaaa tatagctagc tctcttggga acgccttatt agccaagacc atataccttt
    10681 tctaatagag ctaatcaagg tgttatgtta taatagagtg actagctctt gaaggagaat
    10741 ttagaatgac aaaacaaaaa gtagctattt taggacctgg ttcttgggga actgctcttt
    10801 ctcaggttct taatgacaat ggccatgacg tccgtttatg gggaaatatt cctgatcaaa
    10861 ttgaagagat aaataccaag cataccaata gacactactt caaagacatt gtgttagaca
    10921 aaaacattac ggcaactctt gatttaggtc aagccctatc agatgttgat gcggtgcttt
    10981 ttgttgtgcc aactaaagta acacgattag ttgctagaca agttgccgcc attttagatc
    11041 ataaagtagt ggtcatgcat gcctcaaaag gtctagaacc agagacccac gaacgtttat
    11101 caactatcct agaagaagaa attccagctc attttcgtag tgaagtggtc gttgtatcag
    11161 gtcctagtca tgctgaagaa actatcgttc gcgatattac tctcatcacg gcagcctcaa
    11221 aagatattga agctgcaaag tatgttcagt ccctctttag taaccactac ttccgacttt
    11281 ataccaatac cgacgtcatc ggagttgaaa cagctggtgc cctcaaaaac attattgctg
    11341 ttggagcagg agctctccat ggacttgggt atggagataa tgccaaagca gcggtaatta
    11401 ctcgtgggtt agctgaaatc acccgtctag gcgtcaagct tggtgctgac ccgttaacct
    11461 acagtgggtt atctggtgtt ggtgatctaa ttgtaacagg tacttctgtc cattcacgta
    11521 actggcgggc tggagctgcc cttggacgtg gtgaaaaatt agaagatatt gaacacaaca
    11581 tgggtatggt tatcgaaggc atcgcaacaa ctaaggtagc ctatgaaatt gcccaagacc
    11641 tcggagttta catgccaatt acaacagcta tttataagtc catctacgag ggcgctgata
    11701 tcaaagaaag catcctcggc atgatgtcaa atgaatttcg ttctgaaaat gaatggcatt
    11761 aagccattcg tcactaaaat aagaggaggt gctgatgacc aaagtcagaa aagctattat
    11821 tcccgcagca ggtttaggca cacgtttttt acctgcaacc aaggcccttg ccaaggagat
    11881 gctacctatt gttgataaac caactattca atttatcgtt gaagaagctc taaaatcagg
    11941 tatcgaagaa attcttatcg ttactggaaa atccaaacgc tccatagaag atcattttga
    12001 ttctaatttt gaattagaat ataatttaca ggctaaagga aaaatcgaac ttctaaagtt
    12061 agtagacgag accacttcta ttaatcttca ctttattcga cagagtcatc caagaggatt
    12121 aggagacgca gttcttcaag caaagacctt tgttggcaat gaaccttttg ttgtcatgct
    12181 aggtgatgat ctgatggata ttacaaatcc aaatgtaaaa ccattaacca aacaattgat
    12241 cgatgactat gaagaaactc atgctgcaac cattgccgtc atgagggttc ctcacgagga
    12301 tgtctctaac tacggtatca ttgctcctca agcaaaagca gtaaaaggac tctatagtgt
    12361 ggatactttt gttgaaaaac cacaacctca agatgcacct agtgatttgg caattattgg
    12421 acgttacttg ctcacaccag aaatttttag tattttagaa aaacaagaac ctggtgcagg
    12481 caatgaagtt caactgactg atgctattga taccctcaat aagacacagc gtgtctttgc
    12541 tcgtgagttt aaagggaaac gctatgatgt tggcgataaa tttgggttta tgaaaacttc
    12601 tttggattat gccctgaaac accctcaagt caaagatgac ttgaaagcct acatcatcca
    12661 actgggcaaa gcactagaga agacaaaacc ataaaaataa ggagggaaaa tccctcctta
    12721 tttttatggt ttttaagtca tttcatccta ctagccatct gagaagtaac agtgctaacc
    12781 actcacttat tttcataaaa acccaagcac taacacacta accaaaataa taccatagct
    12841 cactaaagcc aacattcgct tggttttctt cactgttatc actctcatct tagttggaaa
    12901 aactatactc agtaacgcac cacccacgac accgccaata tggcctgcca tactcacgtt
    12961 tggcataaaa agattcatca gtaaattgac tacaattaac gtttggtatg acttgcccaa
    13021 atctttcaac gcttggttct tacccaaaaa actcaatact acaatagcag caaaaagtcc
    13081 aaataacgac gtcgatgctc ctgcagctac tgtttcagga gtcaaccaaa aagtaaaggc
    13141 attgcccata acacctgata aaacataaag taataaaaaa cggcgtgagc cccagagatc
    13201 ttcgacgatt tgaccaacaa aatatagtgt taatccattg acaaaaaaat gaccaaagcc
    13261 gatatgaata aatatcggag tcaccaatcg ccacaactgg tctggcattg cttttactag
    13321 taatccaaac atacctccaa cttgataaat agcttgagca ccagttgcta aatgtccata
    13381 taccacttgg attgctataa aaattaagcc tgttaaccct aaaaggaaaa tagtaatagg
    13441 atatcgtttt aataaccgtg tcatctaaga tatacctcct aaacagcaat atcataaatc
    13501 gtttgatttg gcaatcgtat tggcccgctg ataagcttga ttgctaacaa catccactaa
    13561 taaagcttga tcattttgac gttttaagtc tacatccata gttcgtaaag actagaagag
    13621 ttctttgaga atctcttttt tcatcatctt cttatcatac cataaattag gaaaaaggtt
    13681 caaggctgac aagaaactag ctctctcaaa aaagattaaa aataaaggca ttctaaagaa
    13741 tcatttaaga aaactttaag gttactatcg tagaatgaaa acatcctaaa aagcggttta
    13801 cctaaaccgt tgggagaata ggttttcccc ttgttttccc ttttcataaa tctcctgggc
    13861 acctatcttc gtaggtgccc tcccttgttt aaacctgacc agttaaccgg ctggttaggt
    13921 ttttaaattt aaaaagtcaa cagcaaaaat atgatatcac ccgcgtgttc ttagctctct
    13981 caattatgag ctaaaagcac gcatcaatca atctattgat gaagtttgct gctttttgat
    14041 gatctttatt gtgactatac tggtaatcct attgatttta gttgactttt acggtagatt
    14101 cgctacaata tataggatta tacaatttaa tttaaggaga gaaaaattac attgttagat
    14161 tattatttgg aatccgatat tattgaccag aaaatcctac tggctatttt gttgacagaa
    14221 aaagaactga cgatggcagc agcttgttct caaacaactt tgactgcttt aaaaataaag
    14281 cagtatcttc gacaatttaa cgccttattt aaagggtact tacgtattga gcttattaaa
    14341 tctcgtattt attgtgaggt attaaatgat aagcgagaag cattttttta tgacattttt
    14401 gccttatcag atactctaaa aatgttgaca ttcttattac tagataatcc caaacacaag
    14461 tcaatcgctg tttatacacg aaaacaaggg atatcccagt caaaagctta tcgtcttatt
    14521 cataaattaa aacattattt acaagacatt ggtttaaata ttgtagacaa tactgttatc
    14581 ggagatgaac ttaaaatacg gtatttgatt gctcttttac ataaagagta cggcattata
    14641 ttatatgata tacaacctgc agacattgaa acgattcatg cttttatctt tgcagcacaa
    14701 aaaaacttac aaccttctgc ctttttagat agacgcttct tgttttttga tgtgctgttg
    14761 atgttgactt ggaagcgcca tcgctatcct gttcatttgc ctcatttagc tctttttgaa
    14821 catctcaaat ccttgcctat ttttgacaat atcaaaacca ttgccgtgga tgagttagca
    14881 cctcgaactc gtgtcacatt ttcgtctgat gattttgact atctcttttt aatttattta
    14941 acgactgata actccttctt gagtgggtac tggacaagtc atcagcgaca acaactttac
    15001 catttaatca ccaaagatcc cgattatcat ttactaatcc atcgtcttca ggcactagtt
    15061 ggaaaatatt ccgatattca tgagcatatc cctaacttaa ttcctttttt caaaagaact
    15121 ctctacaatt tacaaacttt aattaccttt gatggttatt attttgatca atatcaaggc
    15181 aacaagctac tactagataa gcttgaaacc gttatcaaag attggcttta tgacacaggc
    15241 cgtcaaggca gtattagttc tggtcactta cacttgatgt gtttatacct tgagcagatc
    15301 ttggaaagct ctattgctcc aatcaatatt actgttattg agagccaaga aactgttggt
    15361 aacgttatag ctaatttcat cacctcaact atcccttctt acaaagttga actgtcacgg
    15421 gtcaatatct tatctgataa tatttatccc tatgataaac ctgtagatct ggttgtgacc
    15481 agtcagaagc tcttgccttt cttaaaagaa ctcggtgttt ttcccaaaga gacccgcttg
    15541 tttggtttga gtttggactg tatccaacag cagcgcgaag atttaatcaa aaccatctta
    15601 gccttgcacc agaaccacta ccaaaaacgt ttagaagaac tttggagagt tccttcttaa
    15661 ttttttgaca gtccctccta gatactttca cagtctaggg gggtctttaa ggttgtcact
    15721 tgcaaccttg ataagctctc tttttaacca ttgctattcg cctagaaaca gaactggaac
    15781 tgttttagcg gttcaactcc tcttgtagga tcttcttccc ttttttgaag gtcttatctg
    15841 aaatttggca taataaagtg cgctcccatg ttctggaagc gcactttatt atcgctttta
    15901 cttaaccttg tatccacttg atgatggaag tgccgcccca attcttagat tgcttggcta
    15961 acttttaagc tcttgtacaa ggaagaagtc tttttgacta tctgttgatt agaggcgagc
    16021 attaagctct gctgataatg cttcaaaacc tggttttcca agaagcgcaa acatgttacg
    16081 tttgtaagct tcaacacctg gttgatcaaa tgggttaaca gcattcatgt agcctgaaac
    16141 agcgatggca agctcaaaga agtaaatagt gtaaccaagg gtaaactcgt cttgagctgg
    16201 aagagtgaca aacatgtttg gcacgccacc atctgtgtgg gcaagaagca caccatctgt
    16261 ggctttctta ttaacaaaat caacgtcttt accttgaagg taaccaagac catctaggtc
    16321 ttctgctaac tcaggaatga taacattttt acgagggtta tcaacacgaa taactgtttc
    16381 aaagaggtta cggtaacctt cttggataaa ttgaccaagt gagtgaaggt ctgttgagaa
    16441 gttagctgaa gttgggtaaa tccctttttg atcttttcct tctgattcac cagctaattg
    16501 tttccaccat tcgccaaagt actgaagaga tggctcgtag ttagccaaga tttcagtgat
    16561 gtagccttta cggtaaagca cattacgaac agcagcgtat tggtaagcaa tgttttcaga
    16621 gattttgtct gaagaaaggt ctttacgagc agcatttgcc ccttccataa gagcagtaat
    16681 atctgcacct gatgcagcaa ttggaagcaa accaacagca gtcaagactg agaaacgacc
    16741 accaacatta tctggcacca caaatgtttc ccagttgtta gcgtcagctt ctaccttaac
    16801 agcaccctta actttgtctg tggtagcgta gatacgtttg ttagcttctt cttggccata
    16861 ttttttaacc aaaagttctt tgaacacgcg aaaggcaata gctggttcag tggttgtacc
    16921 tgatttagag atcacgttta ctgagaactc tttgtcttga acgtattcaa ctaaatcggc
    16981 aaggtaagta gatgaaatag agtttccagc gtaaagaatt tgtggggctt tgcgctcttt
    17041 ggcagtttgc aagttagcaa aatgatgatt taaaaaatca atcgctgctt tggcaccaag
    17101 gtatgaccca cctataccga tcaccacaag aacctcgctg tctgccttaa tcttttcagc
    17161 agcagtcaag atacgtgcaa attcctcttt gtcatagttt tctggaagat caagccaacc
    17221 caagaaatct gaacctggtc cagttccttc acgcaataac ttgtcagctt ctgtgacttg
    17281 gccttgtaaa aaatcaattt catgttgtcc cgcaaatgac tcgaggactt ttgaataatc
    17341 aaatgtaata tgtgacatga gtttcgctcc cttaaaattt
//
LOCUS       DAUCWU010000028        16841 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUCWU010000028 DAUCWU010000000
VERSION     DAUCWU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.028"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(112..184)
                     /locus_tag="VGY24_000882"
     tRNA            complement(112..184)
                     /locus_tag="VGY24_000882"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Lys,seq:ctt)
     gene            complement(185..381)
                     /gene="ssrS"
                     /locus_tag="VGY24_000883"
     ncRNA           complement(185..381)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGY24_000883"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(470..1738)
                     /locus_tag="VGY24_000884"
     CDS             complement(470..1738)
                     /locus_tag="VGY24_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ4283874.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(1771..2337)
                     /locus_tag="VGY24_000885"
     CDS             complement(1771..2337)
                     /locus_tag="VGY24_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ4283875.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            complement(2370..4127)
                     /gene="pabB"
                     /locus_tag="VGY24_000886"
     CDS             complement(2370..4127)
                     /gene="pabB"
                     /locus_tag="VGY24_000886"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ4283876.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKEDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHHEMRQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDSLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVQQKTAFLYRHKQIFDLKTTAKVEHKKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLLADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFIQLGQTLYTPPVAVGILPGLFRQELLATGQVQEKELTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            4327..4797
                     /locus_tag="VGY24_000887"
     CDS             4327..4797
                     /locus_tag="VGY24_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ4283877.1"
                     /translation="MATYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            4868..5821
                     /locus_tag="VGY24_000888"
     CDS             4868..5821
                     /locus_tag="VGY24_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ4283878.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            5821..6570
                     /locus_tag="VGY24_000889"
     CDS             5821..6570
                     /locus_tag="VGY24_000889"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ4283879.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTSSMAHELEIIETLPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEQKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLARELKEVKPGAKILFIFGPEGGISPAEITQFEAVGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            6927..9113
                     /locus_tag="VGY24_000890"
     CDS             6927..9113
                     /locus_tag="VGY24_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ4283880.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSTMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTNGNIYAPVA
                     GLVTSVFPTKHAFGLLTNNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGNQPAKTKVATVEL"
     gene            9196..10014
                     /locus_tag="VGY24_000891"
     CDS             9196..10014
                     /locus_tag="VGY24_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ4283881.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYHEG
                     VAILSKQPIHVSDILVSAMDDETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTNQEGYQVMMGSPLNLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(10397..10894)
                     /gene="nrdI"
                     /locus_tag="VGY24_000892"
     CDS             complement(10397..10894)
                     /gene="nrdI"
                     /locus_tag="VGY24_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ4283882.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDRQTDIAQ
                     QLDSL"
     gene            11276..12439
                     /locus_tag="VGY24_000893"
     CDS             11276..12439
                     /locus_tag="VGY24_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4283883.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSSTTM
                     TSEQRQKEIDKIKKDIEEKYPKAEDQKFQEWRWYGSRFKEEEFRNELQEFTKQRLSEI
                     LDLIGKSGIKGDRGEAGPAGPAGPKGDPGKDGAKGDTGPRGERGLQGPEGKPGKAGAP
                     GKDGVQGPKGDKGDTGERGEKGETGATGAQGPQGEAGKDGAPGEKGEKGDRGETGAQG
                     PVGPQGEKGETGAQGPAGPQGEAGKPGEKAPEKSPEVTPTPETPEQPGEKAPEKSPEV
                     TPTPETPEQPGEKAPEKSPEVTPTPEMPEQPGEKAPEKSKEVTPAPEKPADKEANQTP
                     ERRNGNMAKTPVANNHRRLPATGEQANPFFTAAAVAVMTTAGVLAVTKRKENN"
     gene            12691..14910
                     /locus_tag="VGY24_000894"
     CDS             12691..14910
                     /locus_tag="VGY24_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ4283884.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKTDVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            14925..15368
                     /locus_tag="VGY24_000895"
     CDS             14925..15368
                     /locus_tag="VGY24_000895"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ4283885.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     RQLYDSFNEQLAKFVPVERGVFGADMQVSLVNDGPVTIILDTKCH"
     gene            complement(15466..16788)
                     /gene="ska"
                     /locus_tag="VGY24_000896"
     CDS             complement(15466..16788)
                     /gene="ska"
                     /locus_tag="VGY24_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ4283886.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSRPAHGGKTEQGLSPKSKPFATDKGAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKTVHNSAERVNVNYEVSFVSETGDLDFTPSLRDRYHLTTLAVGD
                     SLSSQELAAIAQFILSKEHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHVKNREQ
                     AYGINKKSGQEEKMNNTDLISEKYYVLKKGEEPYNPFDRSHLKLFTIKYVDVDTKDLL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDNNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
BASE COUNT         4908 a         3337 c         3974 g         4622 t
ORIGIN      
        1 aatgaaaatt gagacatgat actaaaagtg gattgacgct tttaattaag ccgattattt
       61 atgaaacaag caaaaaagcc tttatttaaa ggctttttta tgtatagttt atacccccta
      121 cagggctcga acctgtgacc catagattaa gagtctactg ctctaccaac tgagctaagg
      181 aggcaagaat gctgtattgg caccggtgat ccacgttttt gtattgagcc cgcgcagtat
      241 aagcaggtgg gtaacgcgct ctttcttgga agttgcgtcc gcgtgaaacg gcttgcagac
      301 tagaaagagt ctttgtttcc cgaaaataca aagaaatagt cggtcaacac ttagatgtgg
      361 attcgtatgc cacagcaatg tttctgtact tttataatac cactttttaa aattttttca
      421 agcaaaatta tcgatttttg aaatcggttg cgatttttaa aactagcagt cagtctgaga
      481 gcttatcaat acgctcttta tttgaaccaa gtgcgcgttc gtatttaccg gtttcattgg
      541 cttcaaaata atgatgtcct actaatttat ctggtaggta ttgttgtttg acccattttt
      601 cagggtaggc gtgtgggtag aggtaatctt tggcattgcc taggtccttg ctgccagcat
      661 aatgcccgtc tctaagatgc cttggaatgg ggaggttacc tgatgttctc aaatcagcta
      721 aagctgcatc catagctaaa taagctgaat tggatttagg tgatagagcc aaatcaatga
      781 ccacgttagc gataagaata cgagcttctg gaaaaccaat gcgttgagcg gcatctagtg
      841 cggtgacggt atgaacttgg gcgtcagggt tggctaagcc aatatcttcg taagcaataa
      901 tggtcaatcg tcttgctaaa ctaggcaagt ctccagcttc tactaaacga gcggcgtagt
      961 gcaggctagc attgacatca gaacctctaa tggatttttg taaggcagat aaaacatcat
     1021 aatgcccgtc cccgttttta tccatggtaa tataactacg ctgcagacta ttttccattg
     1081 tctctagact aatatgacgg ctactatctt cattaggaga agtagacatg actgctagat
     1141 ccaaggaatt ataagcagat cgtaggtctc catttgtggc ggtaacaatg aaatctagcg
     1201 cctcatcatc aattgtcact aaaaaaggaa atccccgttc tttgtcggaa attgctagct
     1261 gaatggctgt tttaatatcc tcgttagaaa gtggttctaa ttcaaaaatc tggaccctgc
     1321 ttctgatagc gggtgtcact gaaaagaagg ggttctcagt ggttgcgcca atcatgataa
     1381 tggtgccatt ctcaagtaaa ggtaaaagaa aatcttgttt tgtcttgtca aggcgatgaa
     1441 tctcgtctag taataatacc aagccaccag aaaatttagc ttcttccgca atttcttgga
     1501 gacgtttttt gctgtcaatg gtggcattaa aggtccgaaa ggcatatcta gtagtaccag
     1561 caatagcaga agcaatggaa gttttaccga ttcctggagg gccatagagg atcatggacg
     1621 atagtctatt tgcttccacc atgcgacgga tgattttgcc ttcgccaaca aggtgttttt
     1681 gtccgatgac ctctgaaata gttttggggc gcattctgag cgctaaatga tcaggcatgt
     1741 aactgtcctc ctttcttttg ggggatggga ttaacgggga atagctgcga tgaagttggc
     1801 aatcatggtc atgccatcag gagttccgat gctttctggg tgaaattgta gcccaaaaag
     1861 tggcagagtg tggtgttcaa aggccatgat ttcttgatcg tcacagtctc tagcagttac
     1921 gctaaaacct tttggtaact gatccacaac gatggaatgg tagcgcatga cggtgatctc
     1981 ttgtggcagg gagcgaaaaa gactagcagg gccttgcgtt tcaatggtgc tttgtctccc
     2041 atgcatgacg cgtttggcca agcgtaaggt tccccctaaa gtttcagcga tagcttggtg
     2101 tcccagacac actcctaaga taggttttgt ttggtaaaag tcttgaatga gttttggcat
     2161 ttggttggct tccttgggcc aaccaggacc aggtgagagg actagagcgt tagctttttt
     2221 ggccatgtca tataagtttg ggtcttggtt atacaagaca atcgtctcgt caaattcact
     2281 taaatattgg gcgaggttgt aggtaaatga atcgtaatta tcaattaaga gtatcatagg
     2341 ttccttaata acaaaagatg agtggcagtt taggcttgac tcttagctaa gagggcatca
     2401 aggtgagtaa gctcaaggtt gagaggatag aggccacgga cagcatttcc tccaaaaata
     2461 gcgctagcct cttttaaatc agctagtgtt aactcttttt cctgaacttg accagtagct
     2521 agcagctctt ggcgaaaaag tcctggtaaa atccccacag ccactggtgg tgtgtagagg
     2581 gtctgaccca gctggataaa aagattgccg atggacgttt ccaataattg ccccgcttgg
     2641 ttatagaata gctgttcata agatttttgc tcaatatgag gccggtagct ggtcttaaag
     2701 taggtaaaag gcgaagctgt cacgtccttc ttctgtaaag agagttgagc tgtcagaaaa
     2761 tcggctaaca aaggttccaa gggttgatca gagaggctaa tctttccatc cttggataaa
     2821 cgaatcatca agcggtaggc agcattatcc ttattttcca aataggttga cagttgtttc
     2881 tgcaaagctt tctcatcgta agggtaagca aaataagtgg ctgcttcttt aaggcgattg
     2941 aggtgttgct caaggaaggc tatttttttg tgttccacct tggcggttgt tttgaggtca
     3001 aagatttgtt tgtgccgata gagaaaagct gttttttgct ggacttcttc gtattcgtct
     3061 tcccacttgc tttgccaagt gataccgcct ccgacaccat aagtagcttg attatgactg
     3121 agctggatgg ttctaatggg aacattgaag aaacggcgac catcaggtag gcaaatgcca
     3181 atacttccac aataaattcc ccttggtttt ggctctagag atgtgataat tgccatggtg
     3241 gagacttttg gtgcccctgt gatagagcca caaggaaaga gagctgtgag aatgtctatc
     3301 aggtcacagt ccgctttgag gtcaccaaca atagttgagg tcatttgcca gacggtagag
     3361 tatcgctcga cctcacagag actatcaaca caaacactgc ctgtttggca aattttcccc
     3421 atgtcattgc ggagcaaatc cacaatcatc atgttttcag agcgattttt cccgtcagct
     3481 tggagccaat catgttcttg ttgatccagc cagctattaa ccccacgctt ggtggtgccc
     3541 ttcataggcc tagtagttaa cctgttgcct tcctgtttaa agaacaattc aggactagct
     3601 gaaataacgg caaactcatc atgggcgatg taggcattgt agccagcagc ctgttcaacg
     3661 acgagtttat tgtaaatggc taaactatca gctgcattga gttcctgagt taattgaagg
     3721 gtgtagttaa cctgataagt attgccctgt cgcatctcat ggtgaatggt ttcaatggct
     3781 ttttgatagg cttctttttg agtggcgctg acccattggt tagggatagt aatgctgtca
     3841 taatcaagag gaagatcttc tttctgacag gttttgtgca ccgtaaaata ggcaagatac
     3901 tcgttgccta gtctgtcatt atgggtttgt aaggcgttgt caaaaaaagc agctgcttcg
     3961 taactgaggt aacccaccac gtagtacccc agctgttggt agtgttgtac gttttcaatg
     4021 actggaccga cctgatctaa ggacttggct accaattcta ctaatggctc atcaaaaaga
     4081 tagcgttgcc cgagctcttt gaaatcaata atcgtttttc tatgcatacc caaattatat
     4141 cataaaagcg gttgctagag gtgatcctgc cattaagacc ttctcttatg gtcaatatta
     4201 gtcaaatatg ttataattca gttaataaaa gttataacag tacgctcagc atgtcaaagt
     4261 aagtaaacta atgaggtctt acaaagcctt atacagcaac agttagaaag attaggtata
     4321 aaaaagatgg caacatacgg ctttttatca gtattagaag aagaaatgga taagcatttt
     4381 cagtatgact atgctatgga ttgggacaag aaaaatcatg cggttgaagt gacttttgtg
     4441 ttagaagctc aaaataaaga agccataaaa accattgacg atagtggcga agtgacccaa
     4501 gatgatattg tctttgagga ctatgtgctc ttttacaacc ctgccaagtc tcaatttgat
     4561 gcagcagatt atttggttac tattcctttt gacgctaaaa agggcttttc tcgtgaattc
     4621 ttagcttact ttgcgcaatt tctcaacgat gttgctattg aagggcacag cgatttgatg
     4681 gatttcttag cagatgacag caaggctgat ttcttcttgg agtggaacgc gcaagctttt
     4741 gaagaaggtc aacaaggact agaagaagca gcaagttacc cttacccaag gtactaaggt
     4801 gacacacgag atggtgctaa cactattagg acaataacca aaaggtcaaa atagaaagga
     4861 catacttatg gaaacgtggc aagaagtgac ggttcatgtg catcgtgatg cgcaagaggc
     4921 tgtttcacat gtgttgattg agacaggcag ccaaggggtc gctatcgcgg atagcgcaga
     4981 ctatattggg caaaaggacc gctttgggga gttataccca gatgtggagc agtctgatat
     5041 gatagctatc acagcctatt acccaagtag cactaatttg gcggatgtta ttgcgaccat
     5101 taatgaacag ttagcggagt tagctagttt tgggttacag gttggtcagg ttactgtgga
     5161 cagtcaagaa ttagctgagg aagactgggc agacaattgg aaaaaatact atgaaccagc
     5221 tcgcatcacg catgatttaa ccattgtccc tagttggaca gattatgacg cttctgctgg
     5281 ggaaaaggtt attaaattag atcctggaat ggcatttggg acagggacac atcccacaac
     5341 taagatgagc ctttttgcct tggaacaagt ccttcgtggt ggtgagacag ttatcgatgt
     5401 agggaccgga tcaggtgtcc tctcgattgc cagttcctta ctaggtgcta aaaccatcta
     5461 tgcttatgat ttagatgatg tggctgttcg agtggctcag gaaaacattg atttgaatca
     5521 aggaacagac aacattcatg tggctgctgg cgacctttta aaaggggtta gccaagaagc
     5581 ggatgtgatc gtagctaata ttttggcgga tatcttagtc ttattgactg atgatgctta
     5641 ccgtttggtc aaaaaggaag gttaccttat cttatcaggc atcatttctg agaaattgga
     5701 tatggttctg gaagctgcct ttagcgcagg ctttttctta gaaactcaca tggttcaagg
     5761 cgagtggaat gcccttgtct ttaaaaaaac agatgatatc tcaggagtga ttggcggcta
     5821 atgcaacagt attttattaa aggcaaagca gaaaaaaagg taacaattac cgataaagat
     5881 accatcaagc acatgtttca agtgatgcgc ttgactgacg aggcagaagt tgtcttggtc
     5941 tttgatgatg gggtgaagta tttagccaag gtaaccagta gtatggctca tgagttagag
     6001 attatagaga ccttgccaga tcaggtggaa ttgcctgtta aggtgactat tgcttctgga
     6061 tttcctaaag gcgataagtt agatacgatt gcccagaaag tgactgaatt gggagccagt
     6121 gcgctatggg gctacccagc agactggtca gtggtcaaat gggatggcaa aaaattagct
     6181 aaaaaagaag acaaattggc taagattgta ttaggagcgg ctgagcagag caaacgaaat
     6241 agggtgcctg aagttcacct ttttgaacag aaagcagagt ttctaaaaag cctttctagc
     6301 tttgatcata tcttcattgc ttatgaggaa acagctaagg caggtcagct agcgaccttg
     6361 gcgcgtgaat taaaagaagt taagccaggg gcaaaaatcc tctttatctt tgggccagaa
     6421 gggggaattt ccccagctga aatcactcag tttgaggcag taggtgccat caaggtcggc
     6481 ttaggccctc gcattatgcg agcagagaca gcgccgctat atgccttaag tgctcttagt
     6541 tatgctttgg aattgcaggc atcaaaataa gtaagctgct gcttacttta gaaagaagag
     6601 gctaaagaaa cgaacaggca gttcacgatt gtgtcaacgg ccttttttta ttgcttcttg
     6661 ttttttcgaa gagcgttgac gggctcatca ccaaaaaagg aaatgggctc ttgtcatctt
     6721 tttggtgagt tacattacta tttaatgaag aaaaacgact aacatctgtc taagaaaacg
     6781 ctttacaaat tgttgcgagg ctagtataat aaaggtatca gatcaatgca agcgattgca
     6841 tcactgagtt gtagtctata tcattgtgtc acaaaagcgc cttaagggca gctatggcta
     6901 aaaggggata ataaaggaga gatagaatga aaacatcatt caagcaactg tttcgtttcg
     6961 aattttggca aaaatttggg aaatgtctta tggtcgttat tgctgtcatg ccagcggctg
     7021 gtttaatgat cagtatcgga aactctattc caatgattaa ccatgactca gcatttttgg
     7081 catctctagg caacattatc gctcagattg gctgggctgt tattgttaac cttcatttgc
     7141 tatttgcctt ggccattggt ggtagttggg ctaaagaaag agctggaggg gcctttgcct
     7201 caggtcttgc ttttgtcttg atcaatcgga taacaggcgc tttttatggc gtgtctagta
     7261 ctatgctagc tgatccagaa gcaaaaatca caagcctcct tggcactcaa atgatcgtta
     7321 aagattattt caccagtgtc ttggaatctc cagctttaaa cacaggggtt tttgttggga
     7381 ttattgcagg gttcgtagga gcaacggcct ataacaaata ctataattac cgtaaacttc
     7441 ctgaagtttt gaccttcttt aatgggaagc gttttgtacc gtttgtcgtt attttacgtt
     7501 ctatttttgt agcccttatt ttggttgttg tgtggccggt gatccagtct gggattaaca
     7561 gttttgggat gtggattgcc tcttcacaag attcagctcc aatcctagcc ccattcctat
     7621 atggtacctt ggaacgtctc ttgttgccat ttggtcttca ccatatgttg acgatcccaa
     7681 tgaactatac agctcttggt ggaacttatg aggtcatgac aggtgcagca gcggggacaa
     7741 aagtatttgg tcaagaccct ttgtggcttg cttgggtaac tgaccttgtt caccttaaag
     7801 gatcagacgc ttcagcctat agtcacttaa tggatagtgt gactccagct cgttttaagg
     7861 taggacaaat gattggagct accggaacat tgatgggggt tgccttagcc atgtaccgca
     7921 atgtagatgc cgataaaaaa cacacataca aaatgatgtt catctcagca gcggcagcgg
     7981 tctttttgac aggggtaact gagccacttg agtacctatt tatgtttgca gcaatgccac
     8041 tttacatcgt ctatgccctt gttcaaggag cttcatttgc catggctgac cttgtgaatc
     8101 tccgtgttca ctcatttggg aatattgaat tattgacccg tacccctatg gctcttaaag
     8161 caggcctagg catggatgtg attaactttg tttgggtttc tatcctcttt gcagttatca
     8221 tgtactttat tgcggatatg atgatcaaga aaatgcatct cgcaacagct ggccgtttag
     8281 gcaactacga tgctgatata ctgggtgacc ataacactca agcaacacct acccaagtag
     8341 cagatagcaa ctctcaagtc gtacaaatca ttaatctcct tggtggggca ggcaatattg
     8401 atgatgtcga tgcttgtatg acgcgcttac gagtgacggt caaagacccc gctaagattg
     8461 gcgctgagga cgattggaaa aaagctggcg ctatgggctt gatccaaaaa ggcaacggcg
     8521 ttcaagcggt ctatggccct aaagcagata ttttgaaatc agatattcaa gatttgctgg
     8581 actcgggggc tgttattcca gaagtcaaca tgtcacagct cactagcaaa ccgactcccg
     8641 caaaagattt taaacacgtg acagaagatg tgctatcagt ggcagacggg atggttctcc
     8701 caatcacagg tgtgaaagac caggtgtttg cggctaagat gatgggagat gggtttgcgg
     8761 ttgaaccaac aaatggtaat atctatgcac ccgtagctgg ccttgtgacc agtgtctttc
     8821 cgaccaagca tgcctttggt ttactgacaa ataatggtct tgaagtgctg gtgcacgtgg
     8881 gacttgatac cgtcgcctta aatggtgtgc ctttttcagt caaagtctca gaagggcaac
     8941 gggttcatgc aggtgaccta ttagtcgtag cagatcttgc tgctattaaa tcagcagagc
     9001 gtgaaaccac tatcgtggta gcctttacca atacaacaga gatccaagat gtgaccttga
     9061 cttctttagg caatcaacct gctaagacta aagtagccac cgtagaactt taacaaaaca
     9121 tcacgagata gtgataggta atagccatag ctggtttagg ccctatggct cttcttgtct
     9181 tataaaggag gattaatgac caaggtatta acactgaata cccattcgtg gatgcaagcc
     9241 aacactctta aaaaattagt tgctttggct gagcatattt tagctgaaaa atacgatatc
     9301 atttgcttgc aagaaatcaa tcagttaatc gagagcgagc tggccacaga tttaccaaga
     9361 tatcaagctc tttcagggac accaagcatt cataaagacc attttgccct tctcttgatc
     9421 cattatttac aaaaacgagg gcagcactat tattggtctt gggcttacaa ccatattggc
     9481 tatgacattt accatgaagg cgtggcaatc ttatcaaaac aacctattca tgtttcagat
     9541 attctcgtgt cagcgatgga tgatgaaaca gactaccaca ccagacgctc actcatagcc
     9601 aaaaccacgt tagatggcaa agaagtcgct gttgtcaatg tacacctatc ttggtttgac
     9661 aaggggtttt taggggagtg ggagaaatta gaaaaagagc tgctcacact aaactgtccc
     9721 ttgctactta tgggagattt taacaatcca acgaatcaag agggttatca agtgatgatg
     9781 ggtagccccc taaacctaca agatagccat aaggttgctg accatgtttt tggtgatcac
     9841 agtattgtgg ctgatattga tggctggcaa ggtaacaaag aagccctcaa ggtagatcat
     9901 gtctttacca gtaaggactt catcatccgg tcttctaaga ttacctttga aggcggagat
     9961 gcgccagtcg tcagtgacca ctacggttta gaagtgacat tagactggcg ttagagcatg
    10021 tgctaacagg cacaccacat aggagaagcc aagacacata agaaactgcg atcatctagg
    10081 gggattagga gaacacaaag aggtctaacc agctcaaggt agagctggtt agacctcttt
    10141 agtcatgtca gtaaccctaa aaaaccaggg cttttttgtc accaaccatc tgagcttcac
    10201 agaaagtacc gctaagcgtt cagttgcctt gctaaagtct gcaagggggt atttctagga
    10261 gtgcttggtg tgatcccaag ccccacgaag caccatggac cacctcttca aagactgaga
    10321 aatcacaagg cgtggggtaa gagacctccc tagttggtgt cctaaaagcg ctctttttta
    10381 aaacagctat gatcatctat agactgtcta gctgttgggc aatgtctgtc tgtctgtcgt
    10441 tagatgagtg tctggagctg gcctccatga tgacctgggc taaccgcgaa atgtcatcag
    10501 gagtccctcg cagctcaaaa tcacctagca gtggaaagcc aaaacgtttg gcatactgtt
    10561 tggctgtcag gcaatactga tggttaaagt ttttgttgcc tgaaccaata atcccaagac
    10621 aacgctgggc attgccatgg gcagcaataa actctcccaa aggagtggtg aggatctcca
    10681 cctcaccaga gtcaacgcca tttccgcctt ctagataagt gggtagaatc gccacaaact
    10741 cttctttgac gggaaaagtc tcgtgcttca aatctttaat gttaatctgt ttgacatggt
    10801 aatcgtaatt gtctgctaaa taaagggata gccgcttgac aaagctaagg gtgttgccac
    10861 tgaggctaat aaaaacaagg gtaatctgag gcataagtgt ctcctttttt tatcctacaa
    10921 ggtttcttca taagcttacc acaatcaaga ccaacaaaca agtaaagaag aaacctagcg
    10981 gtatccaacc agacaagaag gtacaagcag tcaaaaaaag taacatttca aaaaagaatg
    11041 accttaaaga aaggatgcgc ttgccctcga cttaaaaacg aggcaggcaa gtgccaaagt
    11101 ggccctctga tctccacaaa agagcgatca gtcaaaatct aaaagatgaa caacaaaaga
    11161 gaactttaca atcgttttca cactctatta taatactatt ataaataatc aggtctagct
    11221 accatgttat ttttatctaa aacacaccat ctaataaaga aagagagaac aacatatgtt
    11281 gacatcaaag caccataatc tcaacaaact ggtctggcgc tacgggctaa cctcagccgc
    11341 tgccgtcctt ctagcctttg gaggcggggc aagcagcgtt aaggctgagg tttcttctac
    11401 gactatgacg tcggagcaga gacaaaaaga aattgacaaa attaaaaaag acattgagga
    11461 aaaatatcca aaagcagaag atcaaaaatt tcaggaatgg cgttggtatg gaagtcgttt
    11521 taaagaagag gaatttcgaa atgaactaca agagtttacg aagcagaggc tcagtgagat
    11581 tctagattta attggtaaat ctggaatcaa gggagaccgg ggtgaagctg gtcctgctgg
    11641 tccagcaggt cctaaaggtg atcccggtaa agatggcgcc aaaggtgaca ctggtccgag
    11701 aggtgagcgc ggcctacaag gtccagaagg taagccaggt aaagctggtg ccccaggtaa
    11761 agacggagtc caaggtccta aaggcgacaa aggagatacc ggtgagcgcg gtgaaaaagg
    11821 tgaaaccgga gcaactggcg cacaaggccc acaaggtgaa gctggtaaag acggagctcc
    11881 aggcgaaaag ggtgaaaaag gtgaccgtgg tgaaaccgga gctcaaggtc cagtaggccc
    11941 acaaggtgaa aaaggtgaaa ctggcgcaca aggcccagca ggcccacaag gtgaggcagg
    12001 caaaccaggt gaaaaagctc cagaaaagag tccagaagtg acaccaacgc cagaaacgcc
    12061 agagcaacca ggtgaaaaag ctccagaaaa gagtccagaa gtgacaccaa caccagaaac
    12121 gccagagcaa ccaggtgaaa aagctccaga aaagagccca gaagtgacac caacgccaga
    12181 aatgccagag caaccaggtg aaaaagctcc agaaaagagc aaagaggtaa ctccagctcc
    12241 agaaaaacct gctgacaaag aagctaacca aacgccagaa cgccgcaatg gcaatatggc
    12301 taagacacct gtagccaaca accacagacg tctaccagca actggtgagc aagccaaccc
    12361 attctttaca gcagcagcag tagcagtgat gacaacagct ggtgtcctag ccgttacaaa
    12421 acgcaaagaa aacaactaat cctctgaatt gagagacctt actgataagc aaacataatg
    12481 gagagagccc cttggttctc tctatttttg gtcgggagct ggccgttggt atcatgctca
    12541 cgcctgagag atcccaaaag atatcaaaag cagctcacta accgttttct aagaaaaggt
    12601 cacactaagc gctttcttag cagattctgc cttttgcttt tgtgtgtaaa gtgttaaaat
    12661 agggaaaaga aatgctagat gaggaacgaa atggcaaaaa taatgaacgt aacaggagaa
    12721 gaagtcattg ccttagcggc cacctatatg accaagactg atgtggcttt tgtggcaaag
    12781 gctttagcat atgcaacagc ggcccatttc taccaagtga gaaagtcagg cgaaccctat
    12841 atcgtccatc cgattcaggt ggcggggatt ctggctgatt tgcatctgga tgctgtgaca
    12901 gttgcttgtg gctttttaca tgatgtcgta gaagatacgg atattacttt agatgagatc
    12961 gaagcagact ttggccaaga tgctcgtgat atcgttgatg gtgtcaccaa gttaggtaaa
    13021 gttgagtaca aatctcatga ggagcaactc gccgaaaacc atcgcaaaat gctgatggct
    13081 atgtccaaag atattcgcgt gattttggtg aaattggctg accgcctgca taatatgcgc
    13141 accctcaaac atttgcgcaa ggacaaacaa gagcgcattt cgcgcgaaac catggaaatc
    13201 tatgccccct tggcgcatcg tttggggatt agtcgcatca aatgggaact agaagatttg
    13261 gcttttcgtt acctcaatga aaccgaattt tacaaaattt cccatatgat gaaagaaaaa
    13321 cgtcgcgagc gtgaagcttt ggtagaggct attgtcagta aggtcaaaac ctatacgaca
    13381 caacaagggt tgtttggaga tgtgtatggc cgaccaaaac acatttattc gatttatcgg
    13441 aaaatgcggg acaaaaagaa acgattcgat cagatttttg atctgattgc cattcgttgt
    13501 gtcatggaaa cgcaaagcga tgtctatgct atggttggct atattcatga gctttggcgt
    13561 cccatgccag gccgcttcaa ggattatatt gcagctccta aagctaatgg ctaccagtct
    13621 attcatacca ccgtgtatgg gccaaaagga cctattgaga ttcaaatcag aactaaggac
    13681 atgcatcaag tggctgagta cggggttgct gctcactggg cttataaaaa aggcgtgcgt
    13741 ggtaaggtca atcaggctga gcaagccgtt ggcatgaact ggatcaaaga gctggtagaa
    13801 ttgcaagatg cctcaaatgg tgatgcagtg gactttgtgg attcggtcaa agaagacatt
    13861 ttttctgagc ggatttatgt ctttacaccg acaggggccg ttcaggagtt accaaaagaa
    13921 tcaggtccta ttgattttgc ttatgcgatc catacgcaaa tcggtgaaaa agcaacaggt
    13981 gccaaagtca atgggcgtat ggttcctctc actgccaagt taaaaacagg agatgtggtt
    14041 gaaatcatca ccaatgccaa ttcctttggc cctagtcgag actgggtaaa actggtcaaa
    14101 accaataagg ctcgcaacaa aattcgtcag ttctttaaaa atcaagacaa ggaattgtca
    14161 gtgaataaag gccgtgattt gttggtgtct tattttcaag agcagggcta cgttgccaat
    14221 aaataccttg acaaaaaacg cattgaagcg atccttccaa aagtcagtgt gaagagtgaa
    14281 gaatcgctct atgcagccgt tgggtttggt gacattagtc ctatcagtgt ctttaacaag
    14341 ttaaccgaaa aagagcgccg tgaagaagaa agggccaagg ctaaagcaga agcagaagaa
    14401 ttggttaagg gcggtgaggt caaacacgaa aacaaagatg tgctcaaggt tcgcagtgaa
    14461 aatggagtca ttatccaagg agcatcaggc ctcttgatgc ggattgccaa gtgttgtaat
    14521 cctgtacctg gtgatcctat tgacggctac attaccaaag ggcgtggcat tgcgattcac
    14581 agatcggact gtcataacat taagagtcaa gatggctacc aagaacgctt gattgaggtc
    14641 gagtgggact tggacaattc gagtaaagat tatcaggctg aaattgatat ctatggactc
    14701 aatcgtagtg gtctgcttaa tgatgtgctc caaattttat caaactcaac caagagcata
    14761 tcgacagtca atgctcagcc gaccaaggac atgaagtttg ctaatattca cgtgagcttt
    14821 ggcattccaa atctgacgca tctgaccact gttgtcgaaa aaatcaaggc agttccagat
    14881 gtttatagcg tgaagcggac caatggctaa gaaaggataa aaacatgaaa cttgtcttgc
    14941 aacgggtcaa agaggcctct gtttctattg atgggaaaat tgctggcgct attaatcaag
    15001 ggttgttgtt gttagtaggt gtgggaccag atgataatgc agaagatttg gcctacgctg
    15061 tgcgaaaaat agtcaacatg cgcatttttt cagatgctga tggcaagatg aatcagtcta
    15121 tccaagacat caagggcagt atcttatctg tgtcgcaatt taccctttat gctgacacca
    15181 aaaagggaaa ccgtcctgcc tttactggtg ctgctaagcc agatttggct aggcagttgt
    15241 atgatagctt taatgaacaa ttggcaaagt ttgtgccagt tgagcgtggc gtttttgggg
    15301 cagatatgca ggttagtctc gtcaatgatg gaccagttac gattatttta gacacgaaat
    15361 gtcactagtc tctacaacta agaggttaga aaaaagcaag caactgttga tagcgtgttg
    15421 cttgcttttt ttgtcagtta atctcatcgt tttagaagat cgaggttatt tgtcgttagg
    15481 gttatcaggt ataggtgtcc ctgtataacg caggtagctg taaacttctc gttcttcttc
    15541 ggtataacga tctttatcat aggctaaatg atagctagca ttctctcctt cgggtcgctt
    15601 gcccatataa acagttatga tacggttgtt gtcatcgtga ttatcctcta cttttccagt
    15661 taaggtatag tccataatac caaaagcatc gagattgttg tagagtagtt tagccttatc
    15721 acgaggatcg tataaatctc tgaagtctaa gttacgttcg ctagctgtta agagctgctc
    15781 gctttttagc aagtccttgg tatcgacatc aacgtatttg atggtgaaca gtttcaagtg
    15841 actgcgatca aagggattat acggctcctc ccctttttta aggacgtaat atttctcaga
    15901 gataaggtcg gtgttgttca ttttttcttc ctgaccagat tttttattga tcccataagc
    15961 ttgttcccga tttttgacat ggtaagtaaa ctcttgatcc attggtaaaa tcgtacggaa
    16021 aatgtcattg tcatgagtga cgattgagga gtcacgtttt gtaatgatat aatctggatg
    16081 ctcttttgat aggataaatt gggcaattgc tgctaactct tgtgatgaaa gagagtcacc
    16141 aactgccagt gtggtcaaat ggtatcgatc tcttaacgat ggcgtaaagt ctaaatctcc
    16201 tgtttcggag acaaagctca cttcatagtt gacgttaacg cgttcagctg agttgtgaac
    16261 ggttttaggt tgatacggtc taacgcgcac atgcccgctt agcaaaaatt cttggacagg
    16321 ttgggtcggc aaggttaccg aatcatctcg gtcagcaaag tagaccttgc cgtttcgatc
    16381 agtaatagtt gcatcgcttg caaaatcaat gacctcaaag tagccgtcgt tactgtgaac
    16441 gttagcgatc aattgttctt gaatagcctt taataagtca gctttttcaa gtttatgtgg
    16501 cattgcgcct ttatctgtag caaatggttt tgattttgga cttaagcctt gctctgtctt
    16561 tcctccatga gcaggtcgtg atgttaggtc aatttcaaaa aatttaaggc taatttcttg
    16621 attcgtcccc tcaacagtac cagcaacgct aacaactaat tggctgttgt tgacagatgg
    16681 acggtctagc agccattcag gtccagcaat agcttggaca ggcttgactg ttccaaatgt
    16741 taatgcaaac agcagtgcaa acatcccaaa agataagtaa tttttcatag aaacctccta
    16801 aaagttaagt ttcaatcccc attattagaa taacataata a
//
LOCUS       DAUCWU010000029        16484 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUCWU010000029 DAUCWU010000000
VERSION     DAUCWU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.029"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(108..2099)
                     /gene="uvrB"
                     /locus_tag="VGY24_000897"
     CDS             complement(108..2099)
                     /gene="uvrB"
                     /locus_tag="VGY24_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ4283887.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2292..4466
                     /locus_tag="VGY24_000898"
     CDS             2292..4466
                     /locus_tag="VGY24_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ4283888.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4466..5206
                     /locus_tag="VGY24_000899"
     CDS             4466..5206
                     /locus_tag="VGY24_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283889.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5354..5506)
                     /locus_tag="VGY24_000900"
     CDS             complement(5354..5506)
                     /locus_tag="VGY24_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283890.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTELALTGLGYYL
                     TKHKRP"
     gene            complement(5503..6882)
                     /locus_tag="VGY24_000901"
     CDS             complement(5503..6882)
                     /locus_tag="VGY24_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ4283891.1"
                     /translation="MSHFSDKFMNISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFPKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(7075..7509)
                     /locus_tag="VGY24_000902"
     CDS             complement(7075..7509)
                     /locus_tag="VGY24_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ4283892.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRGLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YYPLDIFQKANHFIVGKLFKKRLECQSQAQLAALIQYAKQLQ"
     gene            complement(7506..7841)
                     /locus_tag="VGY24_000903"
     CDS             complement(7506..7841)
                     /locus_tag="VGY24_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ4283893.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAIELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(7844..8155)
                     /locus_tag="VGY24_000904"
     CDS             complement(7844..8155)
                     /locus_tag="VGY24_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ4283894.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8178..10171)
                     /locus_tag="VGY24_000905"
                     /pseudo
     CDS             complement(8178..10171)
                     /locus_tag="VGY24_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            complement(10277..11370)
                     /locus_tag="VGY24_000906"
                     /pseudo
     CDS             complement(10277..11370)
                     /locus_tag="VGY24_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
     gene            complement(11379..12779)
                     /locus_tag="VGY24_000907"
     CDS             complement(11379..12779)
                     /locus_tag="VGY24_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4283895.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNLKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSNAPEDVKAARIAELFQTKSFLDPSVLVIYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVKGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            13004..13699
                     /locus_tag="VGY24_000908"
     CDS             13004..13699
                     /locus_tag="VGY24_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ4283896.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILITLIFYSILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDSSQYAVVICFATFLINDSYTFYNWL
                     MLQRYQEKKVKKG"
     gene            complement(13704..13862)
                     /locus_tag="VGY24_000909"
     CDS             complement(13704..13862)
                     /locus_tag="VGY24_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283897.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(13931..15244)
                     /gene="obgE"
                     /locus_tag="VGY24_000910"
     CDS             complement(13931..15244)
                     /gene="obgE"
                     /locus_tag="VGY24_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ4283898.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SIIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLEHLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
     gene            complement(15301..15429)
                     /locus_tag="VGY24_000911"
     CDS             complement(15301..15429)
                     /locus_tag="VGY24_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ4283899.1"
                     /translation="MAFGENGPRKKTTFEKVTMFVVILMVLVTVGGLIASALSVLM"
     gene            15782..16484
                     /locus_tag="VGY24_000912"
                     /pseudo
     CDS             15782..16484
                     /locus_tag="VGY24_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5023 a         3458 c         2977 g         5026 t
ORIGIN      
        1 agttaatctt tactttaaaa agttaactta aaattgtcct aataaaaaga attatttgac
       61 tttaacttat atatgttaat aagtataata taagctttat tcaaaaccta gtccattaat
      121 ttaagttcta aaatcaagtc tcgcatttga gctgccaatt cgaaatcaag taattcagct
      181 gcctcttgca tttgtttttg cagagcattg atcgcttctt ttcgttctcc tcttgacata
      241 ctttcataat ccatctcttc ttttgatata tcattgtggc ttgttttact tatactaata
      301 aggcctctga tgtctttttt tatagtttgt gggacgatgc cgtgcgcttt gttatatgct
      361 atttgaatct cacgacgacg agccgtttcg tcaatagctc gctgcataga atctgtcatc
      421 ttgtccgcat acataatgac atgaccatct acgtttcgag ctgctctacc gatggtctga
      481 atcaagccgc gttcattacg gaggaaaccc tctttatcag catccaaaat ggctacaaga
      541 ctaacttctg gcacatcaat cccttcacgg agcagattga ttccaatcaa gacatcaaaa
      601 acaccaagtc gtaaatcacg aataatttct gtccgctcta aggttttaat atcactgtgc
      661 atatacttga ccttgacacc catctctttt agataatcag tcaaatcttc tgccattttt
      721 ttagttaagg ttgtgatgaa cgtccgttca tcacgcgcta cgcgctgatt aatttctccc
      781 aacaaatcat ccatttgccc catacttggc cttacatcaa tttcaggatc aagtagccca
      841 gtcggacgaa taatttgctc aataattgta ttagtctgtg acatttcgta ttcaccaggc
      901 gttgctgaaa catagacaat ttggtgcaca tgactttcaa attcctcgcg tcttaaggga
      961 cgattatcaa gagctgatgg caatcgaaaa ccatagtcaa ctagcatttg ttttctggct
     1021 tggtcaccat tatacatgcc tttgatttgt cccattgtca tgtgactttc atcaatcata
     1081 attaaaaaat cttctgggaa gaaatctaac aaggtataag gaggttctcc tggtgacctg
     1141 ccatccatat gacgggaata gttttcgact ccgctagtat agcccatttc acgtaacatt
     1201 tcaatatcat attccgttct ttggcgtaat cgctgtgctt ctagtagttt accttcagat
     1261 tcaaataact gtaactgctc tgctaactct gcttgaattt tggctattga ttgctccata
     1321 tgctcatcat ttgttacaaa gtgagtagct ggaaataaaa ccaaatgatc aacttctcca
     1381 atagttttcc cagttaaact ttcgatttca catatacggt caatctcatc cccaaaaaat
     1441 tctacccgaa aagcatgttc atccctggat gctggaaaga cctcaacaac atcgccacgt
     1501 acacgaaaac agccgcgttg aaaatcaata tcattacgct caaattgaat atctactaat
     1561 tgatttagca aagtgtcacg tgatatctcc tgacctggac gtaggctgac tgctgaatct
     1621 gcgtactctt ttggcgaacc tagtccataa atacaagaca cagaagctac cactatgaca
     1681 tcgttacgct caagtaaaga agaggtggct gaatggcgaa gtttatcgat ttcatcattg
     1741 actgaactat ctttttcgat ataggtgtcg cttgatggga catatgcttc aggttggtag
     1801 taatcataat aggaaacaaa atattccaca gcattgtctg gaaaaaattc tttaaattca
     1861 ccatataatt gtcctgctaa ggttttattg tgagctatca ctaaggtagg tttatttact
     1921 ttactaatta cttggctcat ggtgtatgtt tttcccgttc ctgtagcacc taggagaatc
     1981 tgagcttttt ctccgccctc gatattatca actaggcttt caatagcttg tggctgatca
     2041 ccagacggtt tatactttga ttttagttta aatggtttat catcacgttt atcaatcatt
     2101 ttataccctc taatagtctt actctcttat agactatttt aacatagata agccagaatc
     2161 acctccaata agcgtaaaaa acatgtcatc tacatgttat gttttatgac atataaataa
     2221 caaatttctt gcttaatgtt caatattttg ttataattaa ccatattttt tagttttata
     2281 aggagattag tatgacgcac aaaataaaag tattgctgct tgcgataatg tctatttttt
     2341 tgacatgcaa tattgcaagt gctgaaacta ttgctattgt ttcagataca gcttatgccc
     2401 catttgaatt taaagactca gatcaaattt acaaaggaat tgacgttgat attattaatg
     2461 aagtagccaa acgtcaatct tgggatttca gtatgagttt cccgggtttt gatgcagctg
     2521 taaatgctgt tcaatctggt caagcgagtg ctctaatggc cggtacaacc attacgaatg
     2581 ctcgtaagaa agtctttcat ttctcagagc catattacga taccaaaatt gtcattgcga
     2641 cacgtaaagc caatgccatc aaaaaataca gtgacttaaa aggaaaaacg gtcggtgtta
     2701 aaaatggaac agcggctcaa gcctttttga ataactataa aaaaaagtat gattatactg
     2761 ttaaaacatt tgacacaggt gatcttatgt ataatagttt atctgctggt tctattgccg
     2821 ctgttatgga tgatgaggcg gttatccaat acgcaatcag ccaaaaccaa gatattgcta
     2881 ttaacatgaa aggagagccc attggaagct ttgggtttgc tgtcaaaaag ggaagcggat
     2941 atgattatct agttaatgat ttcaatacag ctcttaaagc tatgaaagct gatggtacct
     3001 accaagctat catgaccaag tggttaggca cagatgataa agctaccacc agtcaggcaa
     3061 cgggaaatcc atctgccaaa gctacaccta taaaggacag ttataaaatt gtctctgatt
     3121 cgtcttttgc accttttgaa tttcaaaatg gtaagggcaa atacgttggt attgacatag
     3181 aattaatcaa agctattgct aaacaacaag gtttcaaaat tgaaatcgct aatccaggtt
     3241 tcgatgctgc cttaaatgct gtgcaatcta gccaagcaga tggggtcatt gctggtgcaa
     3301 ctattactga cgctcgtaaa gctatctttg atttttctga tccttattat acttctaata
     3361 tcattttagc tgttaaagct ggaaaaaaca tcaagaacta tgaagactta gacagaaaaa
     3421 cagtcggtgc taaaaacggc acctcatctt actcttggtt aaaagaaaac gctcctaaat
     3481 atggttataa tgtcaaggca tttgatgatg gttctagcat gtatgatagc ttaaattcag
     3541 gttctgtaga tgctatcatg gatgatgagg cggttcttaa atacgctatc tctcaaggtc
     3601 gtcgctttga aacacctctt gagggcattt ctactggtga agttggtttt gctgtcaaga
     3661 aaggaactaa tccagaatta atcgaaatgt tcaacaatgg cttagctgct ctcaaaaaat
     3721 ctggtcagta tgatgacatt atagataaat accttgactc taagaaagct gcaactcctt
     3781 ctgaaaaagg cgctgatgag tctactattt caggcctatt atcaaataac tacaaacaac
     3841 tattggcagg acttggaacc acgctcagtt taacccttat ttcatttgct attgctataa
     3901 ttatcgggat catctttggg atgatggccg tgtcaccaac taaatcactt cgactcattt
     3961 caacggtctt tgtggacgtt gttcgaggga ttcctttgat gattgtggct gccttcattt
     4021 tctggggagt accaaacctt atcgagagta tgaccggcca ccagtcaccg attaatgatt
     4081 tcttagctgc tacaattgca ctgtcactta atggcggagc ctatattgct gaaattgttc
     4141 gcggtggtat cgaagctgtt ccagcagggc aaatggaagc tagtcgaagt cttgggttgt
     4201 cttacggaac cacgatgaga aaagtaattc tcccacaagc tgtgaaacta atgttaccta
     4261 actttatcaa tcagtttgtt atttcattga aggatacaac aatcgtctca gcaattggtt
     4321 tagtggaact cttccaaaca ggtaaaatca ttattgctag aaattaccag tcgttccgta
     4381 tgtatgctat tttagcaatt atttacctta tcatgattac actcttaaca agacttgcaa
     4441 aacgtttaga aaagaggctt aactaatgac agaattaaaa attgatgttc aagatttaca
     4501 caaatcttat ggtcaaaatg aagtcttaaa aggaattgat gccaaattct acgaaggtga
     4561 tgtcgtttgt atcattggtc cttctggttc tgggaaatca acctttctac gtaccctcaa
     4621 ccttctagag acgattacca gtggtaaggt catggtggac ggttttgaat tatcagatcc
     4681 taagactaat attgacaagg cacgtgaaaa tatcgggatg gtattccaac acttcaacct
     4741 tttccctcac atgactgttc ttgagaatat tatctttgct cctgttgaat taggtaaaga
     4801 gtctaaagaa gttgctaaaa agcatggcat ggcacttcta gaaaaagtcg gactttctga
     4861 taaggccgac gccttcccag gaagtttatc cggcgggcaa aaacagcgtg tcgccatcgc
     4921 cagaagttta gcaatgaacc cagatattat gctctttgat gaaccaactt ctgctcttga
     4981 ccccgaaatg gttggggacg ttttgaacgt tatgaaagac ttggcagaac aaggcatgac
     5041 catgttaatt gttactcacg aaatgggatt tgctcgccaa gtagctaacc gtgttatctt
     5101 tacagacggt ggtcaattct tggaagatgg tactcctgaa gagatttttg atcatcctaa
     5161 gcaccctcgt ctcattgaat ttttagacaa ggtcttaaac gtctaataga gcagacacca
     5221 tactaacttc caaaaaataa taaggcaagg aaacctgttt ccttgccttt tttggttact
     5281 gttattattc aaaccagcaa aatagggata ctcttatgac tctctccttc ataagagtgt
     5341 cccctatgct tttttatggg cgtttatgtt tagtgaggta ataacctaat cccgtaagag
     5401 ctagttccgt cactatgtgt aagtatttgt taccaaccat cggactaatg gcaatgagaa
     5461 aaaagccaac agccattaaa aaagttgctc catttttttc tgtcatgcaa ggtcactcgc
     5521 tttttcttta ttggccaaaa tgacaaatgg tgcccagata aggaaggcaa caaagaggtt
     5581 aaacagtgaa accaaacctg ccataatatt acctccagta gctaggtaag cataaagtcc
     5641 aacaggggta atccaaggta ctgataagaa taccggtgga atcaaaccta ttgcagtagc
     5701 aaaataagct atgctagcac aaattggtgg cacaattagc catggaatca caaaggttgg
     5761 attcaaaaca attggcatcc caaatgtaat tggttcgttg atattgaaaa cacccattgg
     5821 tgcggacaat ttggcaatgg ttttatgctc ttctcgtttt gagaatatga agatagcaat
     5881 aatcaatgcc aaggttactc ctgaaccacc catttgagca taagcatcaa aggacccgcg
     5941 tgtccaaagg tatggtaaat tagcagcgct atgcgctgta ttgtaagcag ctgtattttc
     6001 tgttaaagct accatataaa taccatccat cactggggct aagacattat gaccatgcaa
     6061 gccgaagaac cagagcagtt gaactaagaa ggttaacaag ataactgaac caataccctg
     6121 tgataaacct agtaatggta attggatata ggttgaaatg acatcactta gggaagaacc
     6181 tgttaatgca aagatgaggt aggcgaaaat tgccgaagcg taaatcgcaa ctgttcctgg
     6241 aataatggca gcaaaggctt tattaaccgc aggtggtaca ttatctggta aggtaatggt
     6301 aatattacgt ttggtcaaag ccgcatacac aaaacttgat aaaaagccaa taattaaagc
     6361 tgtgaacaag cctgtggcac cgacatattt tagagctatt gctccccatt gactagtttc
     6421 aatacttgaa gctccacccg cagtaacgac tgaaagcccc atttctttca aattgctaat
     6481 taggttagca gaggcatttt caagtggtgt cgaaatggtt aaagtctgag gtagggtggc
     6541 aataaatgag ccaaaagaaa tcaatcctcc tgccaatgga ttaatcttat gcataacagc
     6601 taaattgtaa ccgaaagcaa aggcaaaaaa gagcgccatg ataccaattg tcccaaaata
     6661 aacatagcca ttgatgtcga taacaggttg catagctttt ggaaaccctg tccatcccat
     6721 attgtttgga atatctcgta aaaaaacgtt caataagaca gcaactgaac ctgccatggt
     6781 aataggcatc attgaaataa aggaatcacg aatagctact aaatgccgtt gtgacccaat
     6841 ttttcctgaa atattcataa acttatcaga aaaatgcgac ataaatcttc tcctcttttt
     6901 atttttttaa tgtaacaaag gtaaccttaa aagcagcggt gtctgcctaa cacaattatc
     6961 tcaaaatcat ccttgttaaa cgcgatgctt agagataacc ggtaatcggc tcttatccaa
     7021 atgacccctt ctctctcatt caaggtctgt gaataatctt cacaaagacg atggttactg
     7081 caactgttta gcatattgga taagggctgc taactgagct tgagattggc attctagtct
     7141 ttttttgaac agtttgccaa caataaaatg atttgctttt tgaaaaatat ctagaggata
     7201 atagtcataa gaataggtaa ttctagtccg attctccccc agagcctcta atcgataagc
     7261 caattccttt atcccatgat tggaactaaa acaagcagta tataattgcg gagcttctag
     7321 ggccgtaata agccccctca ctgtacgctc tttgtattta ccataatgtt taacatagtt
     7381 tatccccgct ttgatatcat ctcttgacaa ctgtctcaag gtattttgtc gataatcttc
     7441 ttggagcgat atcagcatgg cttcaaaaca atgatgaatg gaaacatctc cttctgctga
     7501 aataatcatg actcacctct ttgtattgct gacattttgt cgtaaacggt aaccaattcc
     7561 ttggctaaat caataaaggc taaggcagtc atcatgtgat cttgcccatg caccatcaat
     7621 aaagacaatt caatcgcatc accagctgct tcttgggaga gcaaatgtgt ctgcgattta
     7681 tgggcaatat ttaacgagtt tgtcgcttct tgaagtaagt gctgagcctc ctcaaactgc
     7741 ccatctcgtg ctgcatgaat agcctctacg gccttgccct tggcttcgcc tccatacatg
     7801 attaagccca taatagcttc taagttggat ggttccgtca tgcctactcc tccatcatcg
     7861 ctagggctgt ttcaaggact ttttcaccgt tcataagacc ataatcagcc atgttaatgg
     7921 catctacttt aatatctggt tcaaacttat ctttaaaatc ctttagtaag aatttgactt
     7981 gtggacctaa caagagaacg tcaatgctat tagcagccac ttcgttgtca gcttcactca
     8041 caggaactgc ccaaatagaa acttcaaggt cacgcgcttg ggctgctttt tgcatcttag
     8101 tcaccaacat actagttgac ataccggcat tacaaactaa cataatagat ttcataaggt
     8161 ttctcctttt aatcgtttta atcatgaata agtggggtaa acactgtcat gaatttggaa
     8221 taatttggtt cttctagtaa cgcttgctga agctccacct gattgacaaa agatgccaaa
     8281 cctggtgata catattttaa cctatgatta tgccctttag acggggataa tagaaagaca
     8341 aaatgaacag cttgatgcgc cttgtcccac tcaagaggct ctcggcaaat agccactact
     8401 acctgttcag aataagtcat gggattagca ggatgcggaa aggcaagcac ttctccatag
     8461 atgactgggc tgtaattttc cctcagtacc atttgatgat aaaagtcctc tacaaacgtt
     8521 tctgttcccg cctcgtcaag acaagcaatc atccgtaaaa gcaagtcttc ctttgagatt
     8581 ttctcgtcta aataaagaaa acgattaggg ctaaaaacac ctgttagaag ctgctctgct
     8641 tttgctaaac tcatctgtga cggtaaactg acctctttct tgataccttc ttgttctcca
     8701 ataaattgtc gaatagtttc aatatcctga tttgacaaaa aaacactgac agtaatcact
     8761 ggtgttaaaa acatcagatt ggcaaggctg atagaggaaa taatcaaatc aacggttttc
     8821 agtcgctctt cggttatttc atagtaactg ataacatcga caatacgcaa acgtccttca
     8881 aattcctttt ccaagcgatt tttcaacatc atggcactac cataacctgt ggcacaaact
     8941 actagaaccc tcaatttatg acggtttgaa taacgctcaa tggccgccat taaatgcaaa
     9001 ctaatataag cccattcatc atctgagaca tcattctttt gacatacaag caaatccgaa
     9061 aaggtctgct tggtaagggt aaatatttca ggatactggc ttttaatttc ctcagttaaa
     9121 ggattagtca gctgaatatg gttttcaaga cgtgtggtca agggcatcat gtgggctaat
     9181 aaaccttgaa ataaattggt atctgtttct aaagtcatgc ctgtcaattg actaatcttg
     9241 gcaatacatg cttttaaatg gtcctgcaac ttttcatcgg tattatcatc ttgccaatgc
     9301 ttaccaacag aatgtttgac ctttagatgc agtgcaatat aattagcctc ctctttagga
     9361 aaacgtatcc ccatcacttc ttccactcga tacaaaatgc gcagagctac ttgatactca
     9421 tcagactgac gaatagctat tggaatggaa aacagttcta atggacaccc cgatcgaata
     9481 cgctgaacca ttagggcaat atgcaagact agattattca tgacaaaatc agaaagtttc
     9541 aacttagcat cccgacattc atccaagaca ataatcatga tttctgcaaa attaatacct
     9601 tcaagaaagg tattacctac catggtggac aaggtattat caaaactagt aacaaaaaat
     9661 agtccatgat aaaatgccta atgtcctctt ctttacctct tatttgaata tgttggtgag
     9721 ttatttcaag ctctaactgg taaggtatta tgcgctcctt aatacctgct aaaacatgtt
     9781 ttaaagttgt ccgactgata tacaattctt gacaaagctc ttcaaaatcc tgaacggcat
     9841 cttcaaaaaa gaatttatga agcacataat cttctcgatc aaccgattct tccacttgag
     9901 tcacatctgc caaacgtttt ttagacgtca cactttcttg ccaaaagatg tcaaatgcca
     9961 tagaatggtc aatttctaag cagtaacctc gaccttgttt agaaatgatg tgagctccgt
    10021 gttcaggaag gctactgatc agttctttga ggtatttcct aaccgtacgg tcactcatac
    10081 caataacctc agccagagtt gcactagaga caaactgctc tttgtgatgg attagaaatg
    10141 ttatgatggc cagttctttt tttgataaca ttagtcctcc ttactacttt agagaacgct
    10201 ttctaccctc ctttagtgta ccaaatattt cctgaaaaag gtgaccatca ttttccgcat
    10261 tgattgtgaa aagaatctat gatggggcaa tctccaaaca aaattcctgc caaggtcgca
    10321 aatcatctaa caaactaaga tagtcctctc tgacctcagc aaccacatta gcttttgaac
    10381 tgaccgtaaa atttttcaaa gcaatttgca gttctccctt atatcgatga tagcggtcat
    10441 tgtcaataat aatgctacct cgtttgacct ccttagattg gtctcttgga gcaatcgatt
    10501 cctgatcata gactagacgt ggcatggttg atcgaatcac ataatcagat acatcgccac
    10561 gataacaatg cggatagcca atgatttgct cctcaacttc tgtcaaatcg atgataggcc
    10621 taaccttgat cgtcgtgact ggtcttgcta aagcttgagt acatgctgtc aattcttctt
    10681 ccgaaatgaa ttggttagat atgaggatat tatcaatggt tcctatcgct ttcattaatt
    10741 ctacttgcag gccaatgggc aagtgcctat ggtcttccac tgtcggcaag ccttctgcca
    10801 gtagccaagg gccttcagat gctgactgag cggtaataaa agcagccgtt tcaatatcat
    10861 gctcatgata aaacgtgaca tatccttaaa atgttgccat gacaaaccgg taaattcatg
    10921 tggataaaaa ttatggcaac caataatatt gctccgttct gcatctgtcg ctagcaatga
    10981 catcaataat tgtttatcag tgctcatatt aagttcaatt ttaagcccaa agggatttct
    11041 tgttaaagtt acaatctctg ctagagggag agcctcgtct aagcgtaagc ccgctaaccc
    11101 gaaggcatga gctcgttcaa taagctgatc tgaccaacca gcttggctaa taaagcttgg
    11161 actaacgtca gcaatcactc gaatgcctaa ttgatttgca taagcaatga gctctgcgta
    11221 gcaatgaaac atctggtgat cagctggtgc cagctgtaag agactcataa acaagcgctt
    11281 ggctccatag ctatgacata agtcaatata agctttactc ttggtaacat cataccgctc
    11341 agggtataga gaaaatccta aatcaaccat attgcctcct atttatcaaa accatttgac
    11401 tcgctcaacg ccttaaacca gtaacctgat ttttttatgg ttcgcttttg actctctaaa
    11461 tccaaagcta ccaaaccata tcggttttta taagcattta accaagacca gcaatcaata
    11521 aaagtccaca agaggtagcc tttgcagttg gctccttctc ccaaagcctt atgaagctgg
    11581 ataagatgat cctcgataaa ggtgatgcgg tagtcatctt gtatctggcc atctgctaaa
    11641 aaagcttctt cacctttgac acccatacca ttttccgtga ctagccattc gatgttacca
    11701 tagtgttctt ttaaattaag agcaatatca taaagtcctg gttcataaat ttcccaaccc
    11761 cggtgaggat tgactttttt accaggcata tcataaggtt caaaataaat atctaagata
    11821 agggggtccc cctcttgctg tgacttacta ggtgcctgaa cccgcaaagg ctgataataa
    11881 ttaactccca aaaaatcaac agtattgttt ttgataatct ctaattcatc agcactatat
    11941 tgaggtaata aatcagcttc ctctaaaatg gaaactaact ctgctgggta aataaccaaa
    12001 actgatggat ctaggaagga tttggtttgg aaaagctctg cgattctagc agccttgaca
    12061 tcttcaggag cattactccg aggataagca ggtgtcatgt taagcacaat actaatctta
    12121 tggtcgggat gaagcttatg acaagctttg accgccagtg aactggccag ttgtgtgttg
    12181 taagccactt gaactgctgc cttggcatct actttacaag ggtagtgata ataacctaaa
    12241 tagccacatt cgactggaac aatcggttca ttaaaggtaa tccaagtgtt tactaaatca
    12301 ccaaaaagct caaaacaggt ttttgcgtag gtttcataag cccagacagt agctttgttt
    12361 tcccagccac ctttttcttg caaggcatag gggaggtcaa aatgatagag gttaacaatc
    12421 aatttaatac cttgagcaat aatgtcttga aatacttctc ggtaaaatgt caccgccttt
    12481 agattaacct ccccgactcc ttctggaatc aatcgagacc actggattga ggtccgaaaa
    12541 atcgtgtggc ctgtttcttt caaaagggca atgtcaccct tatagttttc ataaaaggtt
    12601 gaggtttttt ctggaccaat ttggtgatga aacttagctg actcaatact aaaccagtaa
    12661 tcccagttgc taggtccttt gccatctcca ggaaccgttc cttcgctttg aggcccagat
    12721 gtagaacttc cccataaaaa gccatctgga aattggtaac ggtgttttct ttgtttcata
    12781 tcctttgtcc tttctttgct cacttctctt tttattataa gaagaaaaac aggtgatgta
    12841 aacgctttaa aaaatctgtt ttttccttat tatcacggaa aataagtgat ttaaagcttg
    12901 aaatatttta ttatcttaaa gagcctatct aacctgccct gctacttctt atcaaaacca
    12961 agcccctatt gacagatgcc ttagcctatg tttgaatagg ggtatggctt atttaaacta
    13021 ctttacaaaa accgagtggg cactttggtt aagctctgtc ttggccattt taattagcgc
    13081 ccttttattc ggtaagcaag ctcccttagc tttaatagct tctttgattg gtgtgacctc
    13141 tctcattttt agtgctaaag ctaatcctat tggtcaaggg ctcgtcatta ttttttccat
    13201 tatctatgcc tatctttcat tacgaaacag ttactatgga gagttaatga cctatctttt
    13261 tatgaccctc cctatgacta ttttttcact ttttacttgg ttaaaccatc cttttgaagg
    13321 gaaaaaatca caagtgacta tttcacgatt aacacccact gatcgaagat gtcttttcgt
    13381 ttttactata ctgatcactc ttattttcta cagcatcttg gcttctattc aaacagccta
    13441 tttacttgtc tccactttat cgattgccac ttctttctca gcggtttatc tcagctataa
    13501 gcgcagccct tattttgctt tggcttatgg actcaatgat ctagtcctca ttctcttatg
    13561 gatgcatgct gctcaaacag attctagcca atatgctgtg gtcatctgct ttgccacctt
    13621 tcttattaat gatagttaca ctttttataa ttggctaatg ctccaacgtt accaagaaaa
    13681 aaaggtcaaa aaaggatgag ggcctaagcc tcatcctttt tcaatttttc acgttctaag
    13741 cgcaaacgtc tattgggatt taaaagatta aacaattctt ctaatttctc agcattccaa
    13801 acatctgctg ccgatacaaa atttccatcc ttatccttaa aggatatcgg tttatcaccc
    13861 ataatcagaa acctccatct taaagataac acacaagagc actatcacac tgctattgta
    13921 atggattgcc ttaatccaca aattcaaatt caaacttgcc aatacgaaca ggatccccat
    13981 cttttgctcc acgttcacga agggcttcgt caacccccat accacgcaat tgacgggcaa
    14041 atttcatgat ggactcatca cgttccatat tggtcatgac aaagagatgt tctaacttct
    14101 caccagacaa gacccaagta gcgtcatcgt cacgtgtgat ctcaaagtct ttttcagtct
    14161 cagcaaagcc atagtaagct tcttcatcca ccagatcaga ttcatcatat aataagaatt
    14221 cgtctgtttt agcgagtaat tctgccgttg cttctaacaa gttctctaag ccttgatgtg
    14281 ctaggcttga aatagggaaa atcattggca aatcatcaaa ctcgtcatat tgggcagcca
    14341 attttttctt gaaggctttc aagttttctt gagcctctgg catgtccatt ttattagcca
    14401 caataatctg tgggcgctcc atcaatcgca aattataggt ttccagctcg ttattaatgg
    14461 aaacataatc ttcgtaagga tcacgaccct cactagcaga catgtcaatc acatgaagaa
    14521 taacccgtgt ccgctcgata tgacggagga attgagttcc caaaccaacc ccttggctag
    14581 caccttcaat caaacctggc agatctgcca tagcaaagct atcacctgac tttgtacgaa
    14641 ccattccaag attaggaaca atagtggtaa aatgataggc accaattttc ggtttagctg
    14701 atgacacaac acttaacaag gttgatttcc caacagatgg gaatccgacc aagccaacgt
    14761 cagctaagat tttgagttct aactctaact gacgttcctc acctggctca ccattttcag
    14821 caatctctgg cgcaggatta cgaggagttg caaaacgaat attcccacgg ccaccacgac
    14881 cacccttagc aatgaccact tcttgaccat gttctactaa gtctgtaatt acttttccag
    14941 tttcagcatc acgaaccgtt gtcccttgag gaacaaagac aatcaaatct tctgcaccac
    15001 ggccgtgcat gcctttggtc ataccttttt caccagattt cgccttgaat ttacgattat
    15061 accggaaatc catcaaggta cgcaaacctt cgtccactcg gaagataatt gagccacctt
    15121 taccaccgtc tcctccccaa ggaccgccat ttgggacgta cttttcacga cgaaaagcta
    15181 ccataccatc gccaccacgg cctgcttgga cactgatttt tgccgtatct aaaaacatac
    15241 tcatactttt tttattctct aactttcttt tactttaaaa aaacgccttt cagcgtttcc
    15301 ttacattaat actgataggg cactcgcaat aagcccgcca acagttacaa gaaccattag
    15361 gataacaaca aacattgtca ctttttcaaa ggttgttttt ttgcgaggtc cattttctcc
    15421 aaatgccaca ttaaatacct catctattga tttttcttct tatatcttaa catgaaagat
    15481 cgttttaggc aaatatcaga tgaatattta cgctatgtag gctattactg agtattgtat
    15541 cttgtgaatt tcatatgaat atgatacatt agcatttgca aaataatgtt acagataaaa
    15601 acctcctcgt ctggtgaact gcaccccaaa agttagacac aaaatctaac gattggggtg
    15661 ctattttttt atgaaattta atcaagaaac gaaagttaag atttatgagt tacggcaaat
    15721 gggagagtcc attaaatcca tatcaaaaaa gtttgatatg gcagaatccg atctcaaata
    15781 tatgattcgc ttgattgata ggtatggggt aaccattgtt caaaaatgta agaatcatta
    15841 ttattctcta gaactgaagc aagagataat aaatgaagtc ctgattgacg gccaatctca
    15901 gaaacaaaca tccttagatt atgctttacc aacttctagt atgctctcaa ggtggatagc
    15961 gcaatacaag aaaaacggtt atactattct tgaaaaacca agagggagac cgaataagat
    16021 ggggcgtaaa cgtaagaaaa atttggaaga gatgacagag gttgaacgtc ttcagaagga
    16081 attagaatac cttagagcgg agaatgctgt gctaaaaaag ctgagagaat accgcttgag
    16141 ggacgaagta aagctcaaag agcaacagaa atcattcaag cattaaggaa tcaatttccc
    16201 ttagagatgc tacttgagat ccttgattta tcacgctcca cctattacta tcaagtgaag
    16261 cgactagctc aaggagataa ggatatagaa ctaaagcatg tgattcgaga gatttatgat
    16321 gagcataaag gtaattacgg ttatcgtcgt attcatatgg agttacgtaa ccgaggattt
    16381 gtggtcaatc ataagaaagt tcaacgtctc atgaaagtca tgggcctagc agcccgcatt
    16441 cgtcggaagc gcaagtattc ttcttacaaa ggagaggttg gtaa
//
LOCUS       DAUCWU010000030        16020 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUCWU010000030 DAUCWU010000000
VERSION     DAUCWU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.030"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            63..854
                     /locus_tag="VGY24_000913"
     CDS             63..854
                     /locus_tag="VGY24_000913"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ4283900.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            931..1365
                     /locus_tag="VGY24_000914"
     CDS             931..1365
                     /locus_tag="VGY24_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ4283901.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFSDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            1366..2340
                     /locus_tag="VGY24_000915"
     CDS             1366..2340
                     /locus_tag="VGY24_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ4283902.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            2401..2625
                     /locus_tag="VGY24_000916"
     CDS             2401..2625
                     /locus_tag="VGY24_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ4283903.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            2826..3797
                     /gene="fabK"
                     /locus_tag="VGY24_000917"
     CDS             2826..3797
                     /gene="fabK"
                     /locus_tag="VGY24_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ4283904.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            3822..4760
                     /gene="fabD"
                     /locus_tag="VGY24_000918"
     CDS             3822..4760
                     /gene="fabD"
                     /locus_tag="VGY24_000918"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ4283905.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RHLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKTPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            4750..5484
                     /gene="fabG"
                     /locus_tag="VGY24_000919"
     CDS             4750..5484
                     /gene="fabG"
                     /locus_tag="VGY24_000919"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ4283906.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            5499..6731
                     /gene="fabF"
                     /locus_tag="VGY24_000920"
     CDS             5499..6731
                     /gene="fabF"
                     /locus_tag="VGY24_000920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ4283907.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            6733..7233
                     /locus_tag="VGY24_000921"
     CDS             6733..7233
                     /locus_tag="VGY24_000921"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ4283908.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAIGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            7230..7652
                     /gene="fabZ"
                     /locus_tag="VGY24_000922"
     CDS             7230..7652
                     /gene="fabZ"
                     /locus_tag="VGY24_000922"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ4283909.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            7684..9048
                     /locus_tag="VGY24_000923"
     CDS             7684..9048
                     /locus_tag="VGY24_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ4283910.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAH
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAKAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            9057..9923
                     /locus_tag="VGY24_000924"
     CDS             9057..9923
                     /locus_tag="VGY24_000924"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ4283911.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            9920..10690
                     /locus_tag="VGY24_000925"
     CDS             9920..10690
                     /locus_tag="VGY24_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ4283912.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(10923..12200)
                     /gene="serS"
                     /locus_tag="VGY24_000926"
     CDS             complement(10923..12200)
                     /gene="serS"
                     /locus_tag="VGY24_000926"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ4283913.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDSQLADIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(12592..12951)
                     /locus_tag="VGY24_000927"
     CDS             complement(12592..12951)
                     /locus_tag="VGY24_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ4283914.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(13065..13976)
                     /locus_tag="VGY24_000928"
     CDS             complement(13065..13976)
                     /locus_tag="VGY24_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4283915.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(13993..14802)
                     /locus_tag="VGY24_000929"
     CDS             complement(13993..14802)
                     /locus_tag="VGY24_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ4283916.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(14891..15883)
                     /gene="manX"
                     /locus_tag="VGY24_000930"
     CDS             complement(14891..15883)
                     /gene="manX"
                     /locus_tag="VGY24_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ4283917.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDGLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
BASE COUNT         4856 a         2904 c         3602 g         4658 t
ORIGIN      
        1 tcaaatgatt ttaaagagaa ataatttctt agtcaaataa attatcagaa aagaggattg
       61 ttatgcagtt taaacacatc atctttgatg ttgtagatga tcttgcgaca ttgactttaa
      121 accgtccaga ggtttcaaac ggttttaata ttccaatttg tcaagagatt ctggtagcat
      181 tagcagaagt taaacgcgat acatctgtgc gttttttatt aattaaagcc gttggaaaag
      241 tgttttcagt tggtggtgac ctagttgaaa tgcaagaagc tgttgcaaaa gataatgtgc
      301 agtcgctagt caagattgct gaactggtac aagaaatttc ttttgcaatc aagcatttgc
      361 caaaaccagt catcctttgc gccgatggag ctgtagctgg agcggctttt aatatagctt
      421 tagctgttga cttttgtatc gctagtactc aaacaaaatt tatccaagct tttgtaaatg
      481 ttggactagc accggatgcc ggtggtttat ttctattgac tcgtgcagta ggacttaatc
      541 gtgcaactca ccttgtgatg acaggtgaag gaattactgc tgataaaggt ctcgattatg
      601 ggtttgtcta ccgtacggca gaatctgata aactagacaa agtttgtctt caacttttaa
      661 agcgattgag acgtggctct tcaaattctt atgctggaat gaaatcattg gtctggcaaa
      721 gctttttcac aggatgggaa gattatgcta aggcagagct tgctattcaa gaggagctag
      781 ctttcaagga agattttaaa gaaggtgtca tagcttttgg agaaaggcgc cgaccaaatt
      841 ttcaaggtaa gtaagacagc tattgcttgc aaaatacttt gacctttgat ataatttgat
      901 tgtcaaagtt tattgctagg aggtgtttac ttggaatatg acaaaattaa ccaatactta
      961 gttgatattt tcaaccggat tttggttatt gaagaaatga gcttaaagac gagtcagttt
     1021 agtgatgtct ctttaaaaga gatgcacact attgaaatta tcggcaaata tgatcaggtg
     1081 actccaagtg atatcgccag agaattaatg gtaacgttag ggacagtaac cactagctta
     1141 aataaattag aagcaaaagg ttatatcgca agaacgcgat cacgctcaga taggcgagtt
     1201 gtttacctgt cattaacaaa aaggggtcga cttttggacc gcctccatgc aaagttccat
     1261 aaaaatatgg ttggacatgt cattgctgat atgagtgatg aagagatgca ggcattggtg
     1321 cgtggactag ggaatctaca tcaatttttg gaggatttgg tttaaatgat tttttctaaa
     1381 atcagtcagg tagcccacta tgttccacag cagttagtga ctaataatga tttagctagc
     1441 ataatggaca ctagtcatga gtggatcttt tctcgaacag gaatagcaga acgccatatt
     1501 tctagagatg aaatgactag tgacctagct attcaggttg ctgatcagct tttaactcag
     1561 tctgggttaa aagctgatgc tattgatttt attattgttg caactatcag ccctgatgcc
     1621 actatgccat caacagctgc gaaagttcaa gcagctattg cggctacaag tgcttttgct
     1681 tttgatatga ctgctgcttg tagtggcttt gtctttgcct tggctatggc agataagctt
     1741 attgcatcag gtgcctacca aaatggaatg gtaataggtg cagagacttt atctaagtta
     1801 gtaaactggc aggatcgagc gacagccgtt ctttttggag acggagctgg tggcgtgcta
     1861 ctggaagcca gtaaagataa acatgtttta gcggaaacat tacacacaga tggtgcgaga
     1921 tgtcagagtt tgatttcagg agaaactagt ctttcatctc cttactcaat tggcaaaaaa
     1981 gccatagcga ctattcagat ggatggacgt gccatatttg attttgcgat tagggatgtg
     2041 tcaaaaagca tcctcacact aatggcgcaa tcagatatta caaaagatga catagactat
     2101 tgtttattgc atcaagctaa tcgtcgtata cttgataaaa tagcacgcaa gattgatgtt
     2161 cctcgggaaa aattccttga aaacatgatg cgttatggta atactagtgc tgctagtatc
     2221 ccaatcctct tgtcagaggc tgttcaaaag ggacagatta ggttagatgg tacccaaaaa
     2281 atcttacttt ccggttttgg tggaggtttg acatggggaa gtctaattgt taaaatctag
     2341 ttattcatgt gcctagcaca tttataaaaa caaatttata ttttttaagg agaaataaac
     2401 atggcagtat ttgaaaaagt acaagaaatt attgttgaag aacttggtaa ggaaacagaa
     2461 gaagtaacac tagaaactac atttgatgat ttagatgcag attcacttga tgttttccaa
     2521 gttatttcag aaattgaaga tgcttttgat attcaaattg aaacagaaga aggattaaat
     2581 actgttggtg acctcgttgc ttacgttgag gaaaagtcaa aataaggata aaagtgacaa
     2641 gtaacatcag aagtctatgc aaagcttact gctgttatgt tcatgttcat cactaaaaaa
     2701 acaataagta ttaatcaaac cattagggtg ggattagact ctttttagga tctagctcgc
     2761 tctttcttct tactataata gtttgattat caaattattc tccttatttt ttatagaaag
     2821 ttattatgaa aacacgtatt acagaattac ttaatattga ttaccccatt tttcaaggag
     2881 gaatggcttg ggttgctgat ggtgatttag caggtgcagt ttctaatgct ggtggtttag
     2941 gcattatagg tggtggcaat gctcccaaag aagtcgttaa agctaatatt gatcgtgtca
     3001 aagctattac tgatagacct tttggggtta atatcatgct tttatctcct tttgctgatg
     3061 atatcgttga tttggtcatt gaagaaggtg ttaaagtagt aacaacaggc gcaggaaatc
     3121 caggaaagta tatggaaaga ctgcaccagg cgggtataat cgttgttcct gttgtcccta
     3181 gcgttgcgct agccaaacgt atggaaaagc ttggggtaga tgcagtgatt gctgagggta
     3241 tggaagctgg aggacatatt ggcaagttaa cgactatgtc tttagtgaga caagttgttg
     3301 aagcggtttc gattcctgtc attgcggcag gtggtatagc tgatggtcat ggtgcagcag
     3361 cagcatttat gttaggagca gaggctgttc aaattggaac tcgctttgtt gttgctaaag
     3421 aatccaatgc ccaccaaaat tttaaagata aaatcttagc agcaaaagat attgatacgg
     3481 tgatttctgc gcaggttgtg ggccaccctg tccgttctat taaaaataaa ttgacctcag
     3541 cttacgctaa agcagaaaaa gcatttttaa ttggtcaaaa aacagctact gatattgaag
     3601 aaatgggagc aggatcgctt cgacatgccg ttattgaagg cgatgtagtc aatggatctg
     3661 ttatggctgg ccaaattgca gggcttgtga gaaaagaaga aagctgtgaa acgattttaa
     3721 aagatattta ttatggtgca gctcgtgtta ttcaaaatga agctaagcgc tggcaatctg
     3781 tttcaataga aaagtagata aacacatgaa ggataatgat aatgacaaag acagcctttt
     3841 tatttgccgg tcaaggtgcc caaaaattag ggatggcaag ggatttttat gataactttg
     3901 ctattgtaag aaaaaccttt gatcaagcta gtcaagtatt gggatacgat ttgcgccatt
     3961 tgattgatag tgacgagtta aaacttaatc aaactagcta tacgcaacca gctattttga
     4021 catcatcaat tgctatttac cgtgttttgg gcttacatca cgttaaaccg gatatggtag
     4081 ctggtctttc cttgggagaa tactcagctt tggtagcgtc aggggcactc tcttttgaag
     4141 ataccctatc cttagtagct aagagagggc gcttgatgga ggaagcagca ccacaaggat
     4201 ctggaaagat ggttgccgtt atgaatacag acgtgcaagt catcgaagaa gtctgtcaaa
     4261 tagccgctaa acatggagtg gttgctccag caaactataa tactcctagt caaattgtaa
     4321 ttggtggtca gacagatgct gtgaacgtgg cagttgaact tttaaaggaa aggggagtta
     4381 agcgtttaat ccctttaaac gtgtcaggtc ctttccacac tgctttgtta gaaccagcta
     4441 gccgcttgtt ggctaaagag ttggaaagat acaacttcag tgacttcaag actcctttgg
     4501 ttggtaatac cgaagctaat attatggaaa aagaccgtat cccagaacta ttagcccgtc
     4561 aagtcatgga gcctgttcgt ttttatgaca gtgttgcgac tttagtagaa agtggcataa
     4621 cacaattcat tgaggtagga ccaggtaagg ttttgacagg ttttgtgaag aaaattgata
     4681 aaaatttact atgtactagt gttgaaaaca tggtaagctt acgtttattt ttagatagag
     4741 aggacattca tggaaattaa aggaaaaaat atatttatca caggctcaac gagaggtatc
     4801 ggtttagcaa tggctcatca attcgccagt ttaggagcta atattgtctt aaatggtcgt
     4861 tctgctatct ctgaggagct tgttgctagt tttactgact acggcgttac agttgttacc
     4921 atctctgggg atgtttcaga agcatcagaa gctaaacgta tggtcaatga agctatcgaa
     4981 agtttaggtt cgattgatgt tttagttaac aatgctggca ttactaatga taaattgatg
     5041 cttaaaatga cggaagagga ttttgagcgc gtcttgaaaa tcaatttgac cggtgctttt
     5101 aacatgactc aatcagtgtt aaaaccaatg atcaaggccc gccaaggagc aattattaat
     5161 gtctctagtg tggttggttt aacaggcaat attgggcaag caaactatgc cgcttctaag
     5221 gcaggaatga ttggttttac caagtctgtt gcccgtgaag ttgctgctcg taacatttgc
     5281 gtgaatgcta ttgctccagg atttatcgag tccgatatga caggtgttct tcctgaaaaa
     5341 atgcaagagc aaatcttaag tcaaatccca atgaaacgta ttggaaaagc tcaagaagtt
     5401 gctcacttgg caagcttctt ggtggaacaa gattatatta cgggacaagt tatcgctatt
     5461 gatggcggta tgacaatgca ataaggagac gtagtgaaat gacatttaaa agagtagtgg
     5521 taacaggtta tggtctaaca tctccaatcg ggcatgatcc tgaaacgttt tggaacaatc
     5581 tgaaagctgg ccaaattggt attggcccta ttactaaatt tgacacgact gattatgcgg
     5641 tcaagaatgc tgctgaaatt caggattttc catttgacaa atactttgtg aaaaaagatt
     5701 tgaaccgttt cgatagatat tctctttatg ctctttacgc agcgaaagag gctattaatc
     5761 atgctgattt aaatattgag atgattgatt ccgatcgatt tggcgttatt gttgcttcag
     5821 gtattggtgg aattgcggag attgaagagc aggtgattcg cttacatgaa aaagggccaa
     5881 aacgtgtgaa gccaatgacg ttgccaaaag cgctaccaaa tatggctgca ggaaatgtcg
     5941 ccatgtcttt aaaagctcaa ggagtatgta aatcaattaa tactgcctgt gcttcttcaa
     6001 atgatgcgat tggggatgcc tttcgtgcca ttaaatttgg gactcaagat gtgatgatag
     6061 ttggtgggtc agaggcggcc ataaccaagt ttgccattgc tggcttccaa agtttaacag
     6121 ctctctcaac aactgaagat ccaagcagaa gctctattcc atttgataaa gaccgcaatg
     6181 gttttatcat gggagaagga tcaggaatgc ttgttttaga aagcctagaa catgcgcaag
     6241 aacgcggggc gacaatctta gcagaaattg tgggttatgg aaatacctgc gatgcctacc
     6301 atatgacatc tccaaatcct gagggattag gagctcgtaa agccatccac ttagccttac
     6361 aagaagctgg tattgaagcc agtgctatta attatgtcaa tgcgcacgga acttcaaccc
     6421 cagctaatga aaaaggagag agtcaagcaa ttgtagccgt tcttggtaag gatgttccgg
     6481 tgtcttcaac aaaatcgttt acgggtcacc ttcttggagc agcaggcgct attgaagcta
     6541 ttgccactat cgaggctatg cgtcataact atgttccgat gacggctggg acacaagctt
     6601 tatcagagga tatcgaagca aatgtcatct ttggagaagg aaaagaaaca gctatcaact
     6661 atgccatttc aaatactttt ggatttggtg gtcataatgc tgtattagct ttcaaatgtt
     6721 gggaggagta ggttgaatat tcaagaaatc aaagatttga tggctcagtt tgatacctca
     6781 agcctacgtg aatttttatt caaaacaaat gaaggtgagc taatctttag taaaaacgaa
     6841 caacacctca atgcatcgac atcaaatcaa gaacatgctg tgcctgtacc acaggtacaa
     6901 cttgttccga atccaactgc ttcggaagcg tctagtccag catcagttaa ggatgttcct
     6961 gtagaggagc aaccacaagc agaatctttt gtagcagaag gtgatattgt tgaaagtcct
     7021 ttagtcggtg tagcctatct tgcagctagc cctgacaagc caccatttgt ggcaatcgga
     7081 gataccgtaa aaaaaggaca aacccttgtg attatcgaag ccatgaaggt gatgaacgaa
     7141 gtgccagcgc catgtgatgg tgtgattaca gaaattttgg tatcaaatga agatgtcatt
     7201 gaatttggac aaggattggt gcggatcaaa tgatggatat tagagaaatt caagcagctt
     7261 tgcctcatcg ctacccaatg ttattggttg atcgtgtgtt agaagtgtcg gacgaccata
     7321 tcgttgccat taaaaatgtg actattaatg aacctttctt taacgggcat tttcctcact
     7381 atcctgtgat gccaggtgtt ctgattatgg aagctttggc tcaaacagct ggtgtcttag
     7441 aattgtcaaa agaagaaaat aaaggcaagt tggtctttta cgctggaatg gataaagtga
     7501 agttcaaaaa acaagtcgtc cctggtgatc aacttgtgat gacagcaacc tttatcaagc
     7561 gtcgcggtac cattgctgtc gttgaagcca gggcagaagt tgatggcaag ctagcagcaa
     7621 gtggaacatt aacctttgcc tgtgggcaat gagaatgatt gttctgcgga ggaagaaacc
     7681 cttatgttta aaaaaatctt aattgctaat cgtggtgaaa ttgcagtgcg tatcattcgt
     7741 gctgcacgag aattagggat ttccacagtt gcagtttatt cggaagcgga taaggaagct
     7801 ctgcacacga tattggcgga cgaggctatc tgtattggtc ctgcccgttc caaagaatcc
     7861 tatcttaata tgaattccgt cttgtcagca gctattgtga caggagccca agcgattcac
     7921 ccagggtttg gctttttaag tgaaaactca aagtttgcga ccatgtgtga agagatgaat
     7981 attaaattta tcggtccatc cgcatcggtt atggataaaa tgggtgataa aatcaatgct
     8041 cgttctgaaa tgattaaggc aggagtgccc gttattccag ggtcagatgg tgaagtttat
     8101 aacgctcaag aagccttagc tattgccaat aagattggtt atcctgtcat gctaaaagca
     8161 tcggcaggtg gtggaggtaa agggattcgt aaagtggaga cagaagcaga tttggaggcg
     8221 gctttcaatg ctgccagtca agaagctttg ggagctttcg gaaatggcgc catgtatttg
     8281 gaaaaagtaa tttacccagc tagacatatt gaggtgcaaa ttttgggaga cgctcacggt
     8341 aacatcattc acttgggtga acgggattgc tccttgcaac ggaataacca gaaagtcttg
     8401 gaagaaagtc cgtccattgc tattggtaat accctcagac acgaaatggg gcaagcagcg
     8461 gttagagcag ctaaagctgt agcctatgaa aatgcgggaa ccattgagtt tttgttggac
     8521 gaagacagcg agaaattcta ttttatggaa atgaataccc gtatccaagt tgagcatcct
     8581 gtcaccgaat ttgtgacagg agtggatatt gtcaaagagc aaatcaaaat tgcagcaggt
     8641 cagcctttgg cgattaatca agaagacatc actatcacgg gacatgctat tgaatgtcgt
     8701 atcaatgctg aaaatactgc ttttaacttt gcaccgagtc ccggaaagat tacagacttg
     8761 tatatgccaa gtggtggtgt aggactacga gttgattcag cagtttacaa tggctatgcc
     8821 attcctcctt attatgacag catgattgct aaaatcatcg ttcatggaag caatagattt
     8881 gacgctttga tgaaaatgca acgtgcctta gttgaattgg aaattgaagg cattatcacc
     8941 aatacagact tccagctcga tttgatttca gataagcgag tgattgcagg agattatgat
     9001 acctctttct tgatggaaac gttcctgcca cattacaata aagattagga gaaaatatgg
     9061 ctttatttcg taaaaaagat aaatacatcc gtattactcc caataattcc ctcaaaggat
     9121 ctgtcagcca taatgtccca gaagttccag atgaactctt tgcgaaatgc ccagcttgta
     9181 agcacatgat ttataagaaa gatttgggat tggcaaagat ttgcccaacc tgttcttaca
     9241 attttaggat ttctgcccaa gagcgactga ccttaacagt tgatgaaggc tctttccagg
     9301 aattatttac aagtattgaa accaaggatc cgttacgttt tccaggttat caggaaaaat
     9361 tacaaaaagc caaggaaaca actggtctgc atgaagctgt tttgacaggg aaagctatgg
     9421 ttaaaggaca acaaattgct cttgctatta tggattctca ctttattatg gctagtatgg
     9481 gaactgttgt tggggaaaaa attacccgat tgtttgaatt ggctatcgaa gaaaacctgc
     9541 cggtggtgat ttttacggct tcaggaggcg cccgtatgca agagggtatc atgagcctca
     9601 tgcaaatggc caaggtctct gcagctgtga aaagacattc taatgcaggc ctcttttacc
     9661 tcactatttt gacagatcca actactggtg gggtaacagc tagttttgcc atggaagggg
     9721 atattatttt ggcagaacct caatccttgg ttggctttgc aggtcgtcgt gtcattgaga
     9781 ccacagttcg tgaaaacttg ccagatgatt tccaaaaagc agaattttta caagaccatg
     9841 gttttgtgga tgctattgtg aaacgaacag agttgcgaga caagattgcc cacttagtag
     9901 catttcatgg aggtggccaa tgacagatgt atcaagagtt ttaaaagaag cgcgtgatca
     9961 agggcgttta acaactttgg attacgccaa ccttattttc gatgacttta tggaactgca
    10021 tggcgatcgc catttttcag atgatggtgc cattgtaggt ggcctagctt atttggcggg
    10081 acaacctgtt acggtcattg gtattcaaaa aggtaagaat ttacaggata atttggcaag
    10141 gaattttggc cagcccaatc cagaaggtta tcgtaaagct ttgcgcctta tgaaacaggc
    10201 agaaaaattt ggacgaccag ttgttacgtt tatcaatact gcaggagcct atccaggtgt
    10261 cggtgcggaa gaacgaggac agggtgaggc cattgctaaa aatttgatgg aaatgagtga
    10321 tctcaaggtt cccattatcg ccatcattat tggtgaagga ggctctggtg gtgcattagc
    10381 cttagcggtt gccgatcagg tctggatgct agaaaacacc atgtatgcag tccttagtcc
    10441 agaaggcttt gcttctattt tatggaaaga tggttcaaga gcgacagagg ctgctgaatt
    10501 gatgaaaatt accgcaggcg aattgtatca aatgggcata gtagaccgta ttattccaga
    10561 acatggttat ttttcaagtg aaatcgttga catcatcaaa gctaacctca tcgaacaaat
    10621 aaccagtttg caagctaagc cattagacca attattagat gagcgttacc aacgctttcg
    10681 taaatattaa caacatcctt ttggagtctc cctacgtgtt tatcaagcta agggaggcct
    10741 ttccaaaagg gtttttaact agtcttgtaa ataaggaaat gggtatacta ataagacttt
    10801 tgataaacta gtgtgaaaaa aagtgttgtt aaaggggcaa tgtagtgagt ttcaagtgat
    10861 gtggtggctc tttaaaatag taaaccccag aaatcacttt aatgatctct ggggtgagtt
    10921 tattatttag gtgaaatgac ggtttcgcca cccatgtaag ggcggagtac ttcagggatg
    10981 gtaacagaac catcttcatt ttggtagttt tctaaaatgg ctgctacggt acgaccgaca
    11041 gcaagtcctg acccattaag ggtgtgaaga agtttgacct taccgtcagc ttcatcacgg
    11101 taacggattt gagcacgtcg tgcttggaaa tcttcagtat ttgaacatga tgaaatttca
    11161 cggtaggtgt tttgtgcagg aatccagact tcaaggtcat aggttttagc agctgagaag
    11221 cccatgtctc cagtacaaag ggaaatgacg cgatatggca aaccaagttt ttgaagaata
    11281 ttttcagcat tagcagtcat tttttctagt tcttggtaag attcttctgg tttagcaaat
    11341 ttaaccattt caactttgtg gaattggtgt aggcggataa ggccacgtgt gtcacgaccc
    11401 gcagaacccg cttcagaacg aaaagatggg ctcattgcag taaagtaaat tggcaattct
    11461 ttgccgtcta ggatttcccc acggtaataa ttggtcaaag gcacttcagc agttggaatc
    11521 aggacaaagt tggtatctgc taattcaaag gtgtcctctt tgaactttgg atattgtcct
    11581 gtaccaaaca tggaatcgtg gttaaccatg taaggtgtga tgatttcttg ataaccttct
    11641 ttgatatgtt catccagcat gaagttataa agagcacgct ccaaacgagc accaagattt
    11701 ttatagaata agaaacgagc accagttacc tttgccccac gttcccagtc tagaatatcg
    11761 agatcttcac ctaaatccca atgagcctta acctcaaagt caaagtcacg aggcgttccc
    11821 caacggcgaa tttcgacatt gtcctcttca tcagcaccaa caggaacaga atcgtgagga
    11881 gtattaggta aaacagtgat gatgtcagtc acttgctggt cgatatcagc gagctggcta
    11941 tcaatggttt tgatgtcggc agagactttt tgcatatcag caatttgttg agtggcatct
    12001 tctttttgac gttttgcttg ggcgatggct gcagaagcaa tattacgctc tgcttttaat
    12061 tcttctgatt gcactaatag agcacgacgt ttttcatcaa gttctttaag atgggtcagg
    12121 gtgtcttcag aaacaccgcg gttttttaat ttggcagcga cagtgtcaaa atcggtacgg
    12181 atacgtttaa gatctagcat acagaagctc ctttaagata gttattagta gctgtcagtg
    12241 aaacaaggct aagtaagaca gttggatctt gtgattctct gacgatagaa aaagcacagc
    12301 ttatgaagat gctggagcgc tttggcgcgg ttccatccag cttcacaatc tgtgcactta
    12361 atctctattt tattgatgac acggtagaat ttcatatcat tttccctatc tgttcacagc
    12421 atccacagac tctctgaatg aaaaaaaggt attacttatc cgttaggact attataacaa
    12481 aaaacaagaa aaagtaaact caaatgaggg aatcttttcc actaaatagc aatttagagc
    12541 tactattctt tgctttcttg aaatgataaa tcggcactgc tcattatttt tttaatgtcg
    12601 aaaaaatttg ttaataaagg tttgcatcaa ggttggtaat gtaatcactt tctcattacc
    12661 aatatgttga cgagtgattt ttaagatttt tccagagtct ccagaagcaa ataaaaactt
    12721 gccttggtcc gtaaaaattt caaagtggcg gctaactttt tttcttgaaa cattagcccc
    12781 gatgtgattg atagctgtcc aaggaatttg aatataatca ttcacatttt tatcgttgta
    12841 aaattcaagg gccttgtcac cgagtaaaat atgaccaact ttgcctccca ttccaaggta
    12901 ggagacagct tttgtcttgt attcgacact agtgtttaaa gattgagcca tttaagttcc
    12961 tttcattgcg tagttgtagt tattatacca tattttaagt gggattttcc tgacataaag
    13021 cataaaaaag aagcctgagt tcatctcaga cttccattgg ttgattacat aatacctgca
    13081 aggtgagcaa ggataccaaa tgcaaacaag ccgatgatga ttgcgattgg agaaactttt
    13141 ttcttcaata accacatgca aaggaatgta aggagtaatc cagctaaacc tgggataaga
    13201 gagtccaatt gtccttgaag ggtttgagct tggactttat caaggctcat accgccaata
    13261 gcgttggcaa ggatagtttt caactcagca cctttaacag cgccatctgg gaagaccaca
    13321 taggcaccat ctgacaattg ttttccagga aggtcgatag taaagttgat agatacccaa
    13381 cgttgaacaa gcactgcaag gataaacatt ccaaggattg aagcaccttt agtaatgtct
    13441 tggaggatac cgcctgacat gtctttagta atttcagaac cagctttgta accaaattct
    13501 tgagtatacc ataagaaggc catacggata aggttccatc caaagaagaa cagaagtgga
    13561 ccaacgatgt tacctgttga agccaaagaa gcaccaagtg ccccaaggat aggacgaacc
    13621 gtaaaccaga atactgggtc accgatacca gcaagaggtc ccatcatacc gattttaacc
    13681 ccttggatag ccttgtcatc aattggcgta ccatttgcgc gttcttcttc aagggcaagt
    13741 gttacaccaa tgattggagc tgcaacgtat gggtgagtgt taaagaattc catgtggcgc
    13801 tcaagagcag ctgcacggtc ttcaggagac gtatacaatt ttttaagagc agggataagt
    13861 gcataagccc agcccatgtt ttgcatacgt tcgtagttcc aagaaccttg taagaattgt
    13921 gaacgccacc aaacacgttg acggtctgat ttagttaatt taatttgttc agtcatttgt
    13981 tcttacccct ttctagtagt cttctaagat gtcgccgatt ggatcagctg aacctgctgc
    14041 attaccaccg ttaccacctt ttttagaaag attaaggtag atgaaggcaa ttgctacacc
    14101 gataacaccc atagcgataa gggttaattg gctgatagct gcaagagcaa aacctagggc
    14161 gaagaatggc catacttcac gagtagccat catgttgata accatagcat aacctacagc
    14221 aacaaccatc gcaccaccga tttgcatacc ttcgttcaac cagtttggca tagcattcaa
    14281 aacagattgt acggctgaag taggaacagc aacaagaaga gctgctggga cagcgatacg
    14341 caatccttga agaagaaggg caataaagtg gaagcgttca atacctgcga aattaccttc
    14401 agcggcagct ttgtcaccag cgtgtacaag ggcagttgac aaagtacgaa cgatcattgt
    14461 aaggaaaaga ccagcaacag caagtgggat agctgttgag taagcaaagg taattccttt
    14521 ttgagtaaag tcgccacttt tgaccatgat gatagcagct gcaacagagg caagggcagc
    14581 atcaggggca acggcagcac cgatgtttgc ccaaccaaga gcaagcattt gaagtgtacc
    14641 accaaggatg acaccagctt caaggtgtcc agttacaagt ccaatcaaag tacaggcaac
    14701 aagtggttgg tgcatttgaa attggtcgag gataccttca agaccagcga agaaggcaat
    14761 gataacgacc aaaattgcag aaataatcga aatatctgac atgttgtaat cctttcaaag
    14821 tatagtaaac gaggactttt tgctaataag ttagctagtc atcgttttaa aaccttatag
    14881 cgtttgaaac ttatttaatg ttagcttttt ggataagctc aaataagttt ttcttagaat
    14941 cgttaggaac tttacggacg tcgaatgtaa cacctaggtc gcgtaatttt tcaaaagtag
    15001 caacgtcttc tttgtccatt gataaaacgt tgttaaccat tgttttacca gttgagtgag
    15061 ccattgaacc aacgttcaat tcgttgattt caacaccacc ttcaaccgcg cgaagggcat
    15121 cttgaggggt ttggaaaaga ataagtgcgt gtgtgttacc aaaacgagga tctttagaag
    15181 cttcaatcaa tttgctaatt ggaacaacgt tagctttgac accaccaggt gcagcctgtt
    15241 tgatcaattg tttgcgaagg ccatcttgag caacctcatc agaagcaaca atgatacggt
    15301 cagccttaga agctggagtc catgctgttg caacttgacc gtgtaaaaga cgagtgtcaa
    15361 cacgagcaag attgatcttt aatttaccat ctccgatgac agttccagca ggaatagctc
    15421 cttgaagtgc gttaacaact ttttcagttg cagcagtttc ttcaactggg ttaaggtctt
    15481 caggaagggc tttgataccg tccttagatt ccttaatgat gtttgcagca acttgttcaa
    15541 cacctgcacc agcatccata agacgttcag tgtaagcttg aataagcata ggcaagttga
    15601 gaccagtgat gatcgccatt ttgcggtcag gattttcacc tgccacacga ctagcttggt
    15661 taaatggaga accactccaa aggtcagcta gcacaaggat ttcatcatca gcatcaaatt
    15721 gttggatagc attgttgaag tgtccataca aatcatctgg tccttcgttt ggcatgaaag
    15781 taacaacttg aactttctct tgttcaccaa aaatcataga accagattga tgaatacctt
    15841 cagcaaattt accatggctg gcaataataa taccgatacc cattcttgtt cttcctcctt
    15901 taaaatattt ttcgttgaaa tgaagaagaa taatctcctt ccaaaactaa attttggcaa
    15961 ttctttttca cttaaacgaa attcaagaac gtttctattt taaaacgttt tcactaaaaa
//
LOCUS       DAUCWU010000031        15718 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUCWU010000031 DAUCWU010000000
VERSION     DAUCWU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.031"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(68..2350)
                     /locus_tag="VGY24_000931"
     CDS             complement(68..2350)
                     /locus_tag="VGY24_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4283918.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            2648..2878
                     /locus_tag="VGY24_000932"
     CDS             2648..2878
                     /locus_tag="VGY24_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ4283919.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            3005..3691
                     /locus_tag="VGY24_000933"
     CDS             3005..3691
                     /locus_tag="VGY24_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4283920.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            3691..4425
                     /locus_tag="VGY24_000934"
     CDS             3691..4425
                     /locus_tag="VGY24_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4283921.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            4574..5983
                     /locus_tag="VGY24_000935"
     CDS             4574..5983
                     /locus_tag="VGY24_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ4283922.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATSDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEYVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKVKIE"
     gene            6161..7855
                     /locus_tag="VGY24_000936"
     CDS             6161..7855
                     /locus_tag="VGY24_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ4283923.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSVTKLDAIEAACRTKINQVN"
     gene            8063..8917
                     /locus_tag="VGY24_000937"
     CDS             8063..8917
                     /locus_tag="VGY24_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ4283924.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQNKGIVPGLVVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            9070..10410
                     /locus_tag="VGY24_000938"
     CDS             9070..10410
                     /locus_tag="VGY24_000938"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ4283925.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKIDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            10388..10603
                     /locus_tag="VGY24_000939"
     CDS             10388..10603
                     /locus_tag="VGY24_000939"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ4283926.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            10603..11475
                     /locus_tag="VGY24_000940"
     CDS             10603..11475
                     /locus_tag="VGY24_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ4283927.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            11468..12295
                     /locus_tag="VGY24_000941"
     CDS             11468..12295
                     /locus_tag="VGY24_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEQ4283928.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            12282..12752
                     /locus_tag="VGY24_000942"
     CDS             12282..12752
                     /locus_tag="VGY24_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ4283929.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            12774..14435
                     /gene="recN"
                     /locus_tag="VGY24_000943"
     CDS             12774..14435
                     /gene="recN"
                     /locus_tag="VGY24_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ4283930.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            14607..15563
                     /locus_tag="VGY24_000944"
     CDS             14607..15563
                     /locus_tag="VGY24_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4283931.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLNELDELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELP
                     KPHLRSLSDTALRSGEIRGHLDNKLDTLSVTATKLALTMAQKFDLTTHVYSIGESFSE
                     VLAAHYEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLG
                     MAVAKRKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
BASE COUNT         4900 a         2702 c         3368 g         4748 t
ORIGIN      
        1 tgtttagttt aataaaaaat tgatagaaaa agagagagtc tccttataga aactctctta
       61 gctaacgtta atcgagagac tcttcgttaa gaaaagaatc tccctcttta acagctttaa
      121 tcgtgatatt ttcactgttt gtcatcagtg cacgtaactg tttttcagtt gggtgttcta
      181 aataatagtc tgtaatggca tcttcgatat aatcttgaat agtacgacgg agcggccgag
      241 cgcccatttt aggatcatag cctaaatcta ctaatttttc tttgaccttt tgtgtcacat
      301 caagatgaat cccattatag cctaagcgct cattaacatc ttccagcatt aaatcaacga
      361 tatgcagcaa atgctcttta gataaagctt tgaattcaat aataccatcg aaacgattca
      421 taaactctgg gctgaagaag ttgcttaatt caccaaggac agaacttgtc cgtccttctc
      481 tagcagcacc aaatccgaca gaagcttcgc ttttacctgt tccggcatta gaggtcatga
      541 taataatggt gtccttgaag ctgactgttc gtccttgacc atctgttaaa cggccatcat
      601 caagaacttg taagaacatg tgcatgacgt caggatgggc tttttccacc tcatctaaga
      661 gaataagtga atatggattg cgacgaactt gttcggttag ctgtccagct tcttcatagc
      721 cgacataacc tggaggagcc ccgactaatt tggcgacagc gtgtttttcc atgtattcgg
      781 acatgtcaaa gcgaatcata ttgtttgtcg aaccaaagag ttcaatggct agttgtttag
      841 acaattcagt tttaccaacc ccagtcggtc caacgaataa gaaagaacca atagggcgat
      901 ttggagttcc taatcccaca cggttacgac gaatagcctt agcgatttta tcaacagcat
      961 cgtcttgccc gatcacgtgt gctttcagat cattggctaa gtttacgagt tgagactgtt
     1021 ccttttcttt taagtcccca actggaatat tagttttttg ctctacaata gcttcaatgg
     1081 ttttttcagt aatgatggga atatcttgct catcgacttt ttgagcctgc atttctttgt
     1141 attttgtaat ttgatcgcgg aaataagctg cgcgttcata gtcttcgtct cgagtagctt
     1201 gcgccttgag attttcagct tcaataagac gtttgtcaat ttctttagga tcaacaaagt
     1261 tgagggttag attcattttg gaaccagctt cgtccagaag gtcaatagcc ttatcaggaa
     1321 gaaaacggtc ttgaatatag cggttagata aatgagcggc agcttcaata gcggctgggc
     1381 tatatttgac atgatgatag tcttcgtatt tcggttggat accttttaaa atcgtgatgg
     1441 tttcctcaac agaaggttca tcaactttaa cgggttgcat acgtcgttct aaggcagcgt
     1501 ctttttcaat aatacggtat tcatttaagg tggtagcgcc gaccaactgc aattcaccgc
     1561 gggccaaggc tggttttaaa atattaccag catccatatt gccgtctcct gcagaaccag
     1621 caccgacaat ctcatgaatt tcatcaataa agagaatcac atccttgcga ttgcgaattt
     1681 cttccattaa tttttgcatg cgctcttcaa actgaccacg gatacctgtt ccctgaacga
     1741 ggctaaccac atcaagacga atcacttgct tgccttggag tttttgagga acagtaccat
     1801 caataatttt ttgagccaaa ccttctacaa cagcagtttt accaactcca ggctcaccaa
     1861 ttagcacagg attattttta gtacggcggt tgaggatctc gataacgcgt gtaatctctt
     1921 cgtcacgacc aataacagga tcaatattac catttcttgc aatatctgtg acattaatcc
     1981 caaactcttc taacaagccc tttgcttgct gatttggtgt ttgtggctga gcaggtcgtt
     2041 gtccgttgta gttaccacca tagcgtcctc cgccatttcc attttgccct gcctgagtag
     2101 gtggggtatt tggaagatta ccaaaagctc taaaattatt caaatcacca aaaaagtcat
     2161 caaagaaagg acttgtggat ctatcttgtg ctctatatcc tggggttagg ccatttaaaa
     2221 tagaattggc aggatcggat ttcatgattt gataacaatt ttgacagaga tcaacctgtc
     2281 tttgttttcc attaacactt gtataaagat gaatagtaga ttcgtttaaa ttacaatttt
     2341 gacacagcat aatatacctc cgagggttta tctaaaagtc aaagtcattt agtcaaaaat
     2401 ggtcaaactt tatattcata ttatagcacc acggcttggg aaatcaagta aaaagtttat
     2461 cgctttagcg ttagctttta gagagtaaca tgacagagta actttattaa gtttccaatg
     2521 agcaaatgtt taaaatcatt gaaaagagta aaaaaagtaa aatatcaata aatatattac
     2581 tttccttgtt agtttccctt gttttgtgtt aaaataagag ggataaaaaa cgaataggag
     2641 aaacgaaatg gaatcacatt tggtgagaat catcaatcgt ttggagttga tggtgacaga
     2701 tggcggtaat ctcaagcgaa attttgaacg cgatggtgtt gttgttgcag aagttgcctt
     2761 ttcaaatgat cctgaaaatg gacctgtttt cacattgcgt gatgttgaag cgcgtgagtc
     2821 atattccttt gatagtattg acttgattgc aatggaaatc tacgatttat tatattaagc
     2881 ttgacatagt aaagagactt gcctgaggca ggttttttta ttgtcaaaaa gtattgtatt
     2941 aatattcatc agatggtata attatcttta ttgtaatgaa cctaggactt atgaggagat
     3001 ataaatggat ttgtcatttt tgcccaaata ctgggcctac tttaactacg gtgtacttgt
     3061 caccattatg atttcagtca gcgttgtctt ttttggaacc cttattggtg tcttggtaac
     3121 cctgattaag cgtagtcatg tgaagccgtt gacctgggtc gttaatcttt acgtttggat
     3181 ctttcgggga acacctatgg tggttcaaat catgattgcc tttgcttgga tgcattttaa
     3241 caatatgcct actattggtt ttggggtttt agacttggac ttttcaagac tacttcctgg
     3301 aattattatc atttcattga atagcggtgc ttatatttca gaaattgtta gagcaggtat
     3361 tgaggctgta ccaaaagggc aattagaagc agcttattca ctaggtattc gtcctcaaaa
     3421 tgccatgcgt tatgtgattt tgcctcaggc ctttaaaaat attttaccag ccttaggaaa
     3481 tgaatttatt accattatta aggatagtgc tcttttacaa accattggag tgatggaact
     3541 ttggaatggt gcccaatcgg tggtaacggc tacttattct ccaatttccc ctttactggt
     3601 ggctgctttt tactacttaa tggtcacaac agtgatggca cagttattgg cagtcttaga
     3661 acgtcacatg gcgcaaggag gtaatcattg atgactgagg ctattatttc aattaaagac
     3721 ttgcacaagt attatgggca caatgaagtg cttaagggaa ttgatttaga cattatgcca
     3781 ggagaggttg tggtgattat tggcccgtct ggttctggaa aatcgacctt acttcgaact
     3841 atgaatcttt tggaagtacc aactaaggga cagattaggt ttgaggggat tgatatcacc
     3901 gataagaaga atgatatttt cagcatgcgt gaaaaaatgg ggatggtttt tcagcagttt
     3961 aacctctttc ccaatatgac tattttagaa aatatcactt tatcgccaat caaaactaag
     4021 ggaatggcta aagcagaggc tgacaaaaca gccttgagct tgttggacaa agttggatta
     4081 tcagaaaaag ccaaggctta tcctgctagt ctttctggtg ggcaacagca gcggattgcg
     4141 attgcgcgtg gactggctat ggatccagat gttttactct ttgatgaacc aacttcagct
     4201 ctagacccag aaatggtggg tgaggtcttg gctgtcatgc aagatttggc taaatctggg
     4261 atgactatgg ttattgtgac tcatgaaatg ggctttgcta aagaagtcgc agaccgtgtg
     4321 atttttatgg atggcggcgt tattgtggaa gaaggaagtc ctaaccaact ttttgatctg
     4381 actaaagaag aacgtacaaa agattttttg agtagagtat tataaatctg ttctatgaat
     4441 tataaaagga agtcctaaat gatttaggac ttccttttat aatttgctaa ggcattttat
     4501 aggagattac ttttcttttt gttaaaataa aaagaaatag ttgcattatt tgcatagaag
     4561 aaagggatct gttatggttt cagtgatgtg gtttcgtaga gatttaagga ttgatgatca
     4621 aaaagcttta gctcatgcta ttgctagtca atcaccaacc ttatgtgtgt ttcaatttaa
     4681 caaggagcaa ttaaatctgc tacatagtcg gaatcaatct agttttatta ctagcgtttt
     4741 ggcttttaga aagttattaa aatgtgaagg tattgactta tatctaatgt atggagactt
     4801 aatgacctgt tttgagcaat tattgacaca attaaaagac tggacagatg tgtattttaa
     4861 ctatgatgaa tctggatatg gtcgtttacg tgatcaaaaa gcagcacaat ttttcaaaaa
     4921 aaatgggatt gctgttcata cctaccaaga tcactattta catggtagtc aagagatcat
     4981 taaccaatca ggtcagcctt ataaagtttt tacgccctac tataggattt ggcaaaatta
     5041 tccgaaagag actcctatta aagtagaact atcgcaaggc aggtggttaa atcttgagac
     5101 accagatgat gttttaagga ctgttgagag ttttaaagat gaaaaatatc aggatgttgc
     5161 aacttctgat gaagcgtcaa aacaactaaa tcgatttatt caagaccaat tagcagctta
     5221 tcacgctaat cgagattttc cagcgcagct agggacgagt cggttgtctc cttttttaag
     5281 aataggagct attggcattc gcacggtata tcacgctgtg cgtcaagcac caaatagtct
     5341 agggcaagct acttttttaa aagaattagc ttggagagac ttttacaata tggtctatgt
     5401 ggcttatcct gatcagaaaa ctcagcctat ccaaaaagcc ttttctcaaa tcgaatgggt
     5461 taataatcct gactggtttc agttgtggaa agaaggtaaa acgggttacc ctattgttga
     5521 tgctgccatg cttcagttac aaaagacagg atggatgcat aatcgtttac gaatgatagt
     5581 agcttctttt ttaactaaag atcttttatg tgattggcgt ttaggagagc agtattttca
     5641 acaacaacta attgattatg atgctgccag taatatcgga ggatggcagt gggctgcttc
     5701 cactggaaca gatgctgttc cttattttag aatatttaat cctgttactc aaggtaagcg
     5761 ctttgatccc aaaggcgagt ttattaaagc gtatttgcct caactagaat acgttccaga
     5821 gaagtatctc cacgaaccgt ggaagatgcc aaaaaacctt caagagagtg tgtcctgtat
     5881 tattggcaca gactatcctc aaccaattgt cgatcatgct aaacaacgtg aacaagctat
     5941 tgcaaagtat gagtgggcaa aagaaaaagt aaaaatagaa tgactgttct cattataaga
     6001 gtttaaacaa aaagttatca tgaattaaga ttatttaatt taaaaaataa gttgacttta
     6061 tttagattat ttgatactat tcagttgtct cccttagaga aagcgatttc ttgttttgga
     6121 gctgattttt accatttggt cttagaatag gatgagtaat atgacttaca acgaggtata
     6181 tcaagaatgg ttgcacaata atgatcttag tgatgatatt aaagcagatt tagcagccat
     6241 aaaagacaat gaggctgaga ttcaagatcg tttttacaaa acacttgaat ttggaacagc
     6301 agggctaaga ggaaaacttg gagcaggcac caatcgcatg aatacctata tggttggtaa
     6361 ggcagcacaa gctttagcta atactattat tgatcatgga cctgaagcag ttaaaaaggg
     6421 cattgcggtt agttatgatg ttcgctatca atctagaaca tttgcagaat taacatgctc
     6481 tattatggca gctaacggta ttaaggcgta cctttataaa gggattcggc caacaccaat
     6541 gtgttcgtac gctattcgtg ctttgggatg tatctctggt gttatgatta cggctagtca
     6601 caatccccaa gcttacaatg gttacaaagc ttattggcaa gaaggttctc aaattttgga
     6661 tgacattgcg gatcaaattg cacaacacat ggctgcacta actcagtatc aggaaatcaa
     6721 acaaatgcct tttgaaaagg ctctggacag tggacttgtt acttatattg atgagagtat
     6781 tgaagaagca tataaaaaag aagtgcttgg tttaacgatt aatgatactg acattgataa
     6841 gtcagtccga gtagtctata cccctttaaa tggtgttggg aatctaccag tgcgtgaggt
     6901 tttaagacgc cgcggttttg aaaatgttta tgtggtacct gagcaggaaa tgcccgatcc
     6961 tgattttaca acagttggct atcctaatcc cgaagtccct aaaacttttg catattcaga
     7021 aaaattaggt aaggcagttg atgctgatat tttgattgca actgatccag attgtgaccg
     7081 tgtggcttta gaagttaaaa atgcagtcgg tgactatgtt tttctcaatg gtaataaaat
     7141 cggagcgctt ttatcttact atatcttttc acaacgattt gacttaggca atttaccggc
     7201 taatcctgtt ttagtgaaat ccattgtaac aggagacttg tcacgggcta ttgctagcca
     7261 ttatggtatt gaaaccgttg aaacattgac tggttttaag aatatttgtg gcaaggcaaa
     7321 cgaatatgat gtgacgaaac aaaaaaacta tctgtttggt tatgaagaaa gtattggttt
     7381 ttgctatggc acttttgttc gtgacaaaga cgctgttagc gcctctatga tgattgtaga
     7441 aatggcagct tactacaaga aaaaggggca gaatttacta gatgttttgc agactattta
     7501 tgcgacattt gggtattata atgaacgtca aattgccttg gagcttgagg ggatagaagg
     7561 acaaaaacgc attgcgagga taatggaaga tttcagacag acaccaatag ctagtgttgc
     7621 agagatggcg ttagacaaga caattgattt tattgatggc tatcaagatt ttccaaagca
     7681 aaactgtctc aaattttatt tggatgatgg ttcttggtat gcacttagac catcaggaac
     7741 agagcctaaa ataaaatttt atttatatac tattggccaa acacaagaaa atagtgtaac
     7801 aaaattagat gctattgaag cagcgtgtcg cacaaaaata aatcaggtta attaaacatc
     7861 aaaaatggat aacactttta cactattttt ccatataaac aactacaaaa ggaggtgaga
     7921 cttttaggca ccttcttttt cttgtaggtg ccactaacca atttaatcga ctcaaaccaa
     7981 tactaaaaaa taagtaagag tataaagaac aggcatctat ttttttgtta taatagaaaa
     8041 atgaaattaa agggagatgt caatgacaga actaattgat ggtaaagcct tagctcaaaa
     8101 gatgcaacaa gagttagcag ctaaagtcaa caatctaaaa caaaacaaag gaattgtacc
     8161 aggcttagtc gttattcttg taggtgatga tcctgctagt caggtgtatg tccgtaataa
     8221 agagcgtgca gctcttactg taggttttaa aagtgagacc gttagattat cagaattcat
     8281 ttgtcaagaa gagcttattg cagtaatcga acgttacaat gcagataaca ctattcatgg
     8341 tattttagtg cagttacccc tgccaaatca tattaatgat aaaaaaatta ttctcgccat
     8401 tgatcccaaa aaagatgtgg atggttttca cccgatgaat acaggtcacc tttggtcagg
     8461 acgtcccttg atggttcctt gtactccatc agggattatg gaattgcttc gagaatataa
     8521 tgttaacctt gaaggtaaac atgccgtcat tattggcaga tcgaatatcg ttggaaaacc
     8581 aatggcacag ctcttactgg acaaaaatgc aacagtcacg ttgacacatt caagaacacg
     8641 tcaattagaa gaagtatgtc gctgtgcaga tgtgttgatt gtggcaatcg gacaaggtca
     8701 tttcataaca aaacaatata taaaagatgg tgcgatagtg attgatgtgg gaatgaaccg
     8761 tgatgataat ggcaagctaa ttggagatgt ggcctttgat gaggtggcag aagttgcagc
     8821 gaaaatcacc cctgtaccag gaggtgtcgg tcctatgacg attgctatgt tgctagagca
     8881 aacttatcaa tctgccctcc gtagtactca taaatagtta taattagaca attcaacata
     8941 agagaatata agaagtcagc aatggcttcc ttttttgttt ttctgctcac tatttatcca
     9001 gaaaaatggt ataattgcat tagtatgcaa agggactatg tcaggtccaa ctaagtgaaa
     9061 ggtggcttga tggcagatta tttaaccgtc actcatttga cgaaatattt aaaattaaaa
     9121 tttgaccgtg acccttattt ggaacgggtt tatctgactg gtcaagtgtc caattttcga
     9181 aaacgaccaa ctcatcaata tttttcctta aaagatgaaa gtgctgtgat tcaagccacc
     9241 atgtgggcag gagtctataa aaaactagga ttcgacctag aagaaggcat gaaaattaat
     9301 gtaattgggc gagtccaact ttatgaacct agtggctctt actctatcgt gattgaaaag
     9361 gcagagccag atggtatagg tgccttggct ttgcagtttg aacaattaaa gaaaaaatta
     9421 acggcagaag gttattttga gcaaaaacac aagcagccct tgccacagtt tgtatcaaaa
     9481 attggggtca ttacaagtcc tagtggtgct gtgattcgag atattatcac aaccgtttca
     9541 cggcgttttc caggggttga gattttatta ttcccaacca aagtacaagg tgatggagcc
     9601 gcccaagaag tagtggctaa tattcgaaga gccaatcaaa gagaggattt ggacttgctc
     9661 attgtcggcc gtggaggtgg ctcgatagaa gacctttggg ccttcaacga ggaaatagtg
     9721 gttcaggcta tctttgaatc gcaacttcca gtgatttcga gtgtgggtca tgaaacagac
     9781 acgactttag cggattttgt ggcagatcgc agagcagcca caccaacagc tgcagcagag
     9841 ttagcaacgc ctattacaaa aatagatctt atgtcttgga tagtagagcg gcaaaatcgt
     9901 tcctatcagg cctgcttgag gcggattaag caacggcaag agtgggtgga taaactctcg
     9961 caatctgtta tttttaggca accagaacga ttatatgatg cctacctgca aaaaattgat
    10021 cgccttagca tgaccctgat gaatacgatg aaagatcgtc tgagttccgc caaagaaaac
    10081 aaggttcagt tggaccattc cttggccaat agtcaattac agacaaaaat cgagcgatac
    10141 caagaccgtg ttgctaccgc taagcgttta ctgatggcta atatggctag tcaatatgat
    10201 agtcagctgg ctcgctttga aaaagcacag gatgctttgc tatcactgga tgccagccga
    10261 attatcgccc gtggttatgc aatgattgaa aaaaatcaag cactggtagc ctctgtaagt
    10321 cagataacaa agggtgatca gttaaccatt aagatgcgtg atggacaatt agatgtagag
    10381 gtaaaagatg tcaaaaacga aaacatttga agaaaattta caagatttag agactattgt
    10441 gaacaaactt gaaaatgggg atgtcccttt ggaagaagct atttcagaat ttcaaaaagg
    10501 aatgcttctc tcaaaagaac ttcaaaagac cttacaagcg gcagaaaaaa cactcgtcaa
    10561 agtgatgcaa gctgatggca cagaagtaga tatggatgat taatggacaa attagctaga
    10621 attgacgaag ccattcgtcg ctactataag acgactagta acggtgtatc tgaagagttg
    10681 attgatgcta tcttgtattc tgttgacagt ggtggcaagc gcattcgccc acttatctta
    10741 ttagagatga ttgagggatt tggggtatcg ttacagaatg ctcattttga tttggctgca
    10801 gcccttgaaa tgattcatac aggaagtctg attcacgatg atttgccagc catggataat
    10861 gatgattacc gacgtggtag actgaccaat cacaaacaat ttggagaggc tactgctatt
    10921 ttggcgggag atagcttatt tttagacccc tttgggttaa tagctcaagc tgaattaaat
    10981 agcgaggtta aagtagcctt aatccaagaa ctttccctag cttcaggaac ttttggcatg
    11041 gttggtggtc agatgctaga tatgaaaggg gaaaatcaag ctttgagtct ccctcagttg
    11101 tcactgattc acctcaataa aaccggaaaa ttattaactt ttccttttaa agcagcagct
    11161 cttgtaacag aacaggctat tactgttcgt caacaactag agcaggcggg aatgctcatc
    11221 gggcacgcct ttcagattag ggatgatatt ttagacgtga cagctagctt tgaagatctt
    11281 ggaaaaacgc ctaaaaagga tttatttgca gaaaaagcta cttacccaag tttactgggg
    11341 ttagaagcct cctaccaact gttgacagag agtttagatc aggctttgac gatttttcag
    11401 acactcgaaa gcgatgtagg ctttaagcct caaataatta caaaactgat agaagggtta
    11461 cgacttaatg cctaaagaaa gagtagatgt gttagcctat aagcaaggat tatttgagac
    11521 cagagagcaa gctaagcgtg gtgtcatggc aggcttagtg gtctctgtga ttaatggcca
    11581 acgctatgac aagccaggtg acaaaattga cgatgggact gagttaaaac ttaaaggtga
    11641 aaaactcaaa tacgtcagtc gtggtggatt aaaactggaa aaggggctgc acgtttttgg
    11701 tgtatcggtt gctaatcaaa ttgggattga tattggcgct tcaacaggtg gttttaccga
    11761 tgttatgtta caagatgggg ccaaactagt ctatgccgtc gacgttggga ctaaccagtt
    11821 ggtatggaaa ctcagacaag atccacgagt aagaagtatg gaacagtata actttcgtta
    11881 tgctcagcca gaagacttta atgagggaca gcctgtattt gccagtattg atgtgtcctt
    11941 tatttctctc agcttgattt tgccagccct gcataacgtt ttgtcagatc agggacaggt
    12001 tattgctctc attaaaccgc aatttgaagc tgggcgcgag cagattggta aaaaaggcat
    12061 cgtcaaggac aaacagattc atgaaaaagt gattcaaaag gtcatggatt ttgcctcagg
    12121 ttacggattt acagttaagg gacttgattt ttcccctatt cagggtggtc atggcaatat
    12181 cgaattttta gctcatcttg ctaagtcaca gacacctgaa acgttagccc cgcatttgat
    12241 tcagaaagtt gttgccaagg cacataagga gtttgagaaa catgaaaaag agtgaacgac
    12301 ttgaattaat caaaaaaatg gttttgacgc atccaattga gacgcaacat gacctcttaa
    12361 gattattggc agagcatggg ttagaattaa cccaggctac tatttcaaga gacatgaatg
    12421 aaattggtat agtaaagatt ccttctggca gtggacgtta catctatggt ctttctcaag
    12481 atagtggaaa aaagatcgtt caaggaccta gatcgataaa gagcactatt ttagctgtat
    12541 ctgacaaaac aaaaggttta gaacaacacc tctatttaaa agtcgtacct ggcaatagta
    12601 aattaatcaa acgttattta ttagcagatt tttcaaaagc tatttttagt cttattgctg
    12661 atgatgatag tttattgtta attgcaaagt ctccttcaga agcagatatg atacgccaag
    12721 aaattttgct ttggatgcag ggtataacct agaaaaaaat aggggagaaa gtgatgttat
    12781 tagaaatttc tattaaaaat tttgctatta ttgatgaaat ttccctaaat tttgaaaatg
    12841 gtatgacggt tttgacgggt gaaactggag caggaaagtc tattatcatt gatgctatga
    12901 atatgatgct tggtgcgcgt gctagtacag aagtgattcg tcgtggagct aataaagcag
    12961 aaattgaggg gttcttttcg gtagatgcta ccccagaact ggttgcgtgt ctggaatcat
    13021 caggtattgc tatggaagaa gaactgatta ttcgccgaga tatctttgcc aatggcagaa
    13081 gcgtgagtcg cattaatggt cagatggtta acctagccac cttaaaacag gtcggacagt
    13141 ttttggtaga tattcatggg caacatgacc aagaagaatt aatgcgacca cagctccacc
    13201 agcaaatttt ggacgctttt ggtgataagg cttttgagca attgaaagag aactatcagc
    13261 ttatttttga tcgttataaa agcttgcgtc gccaggttat tgacaaacaa aaaaatgaaa
    13321 aggaacacaa agatcgtatt gatatgttgg cctttcagat agcagaaatt gaagctgctg
    13381 ccttgagtcg aggtgaggac gaccggttaa atcaagaacg tgatcgctta atgaaccaca
    13441 aacaaattgc tgataccctg accaatgcct acgtcatgct agataatgac gatttttcaa
    13501 gtctatccaa tattcgctct agcatgaatg acttactatc aattgagcag tttgattcag
    13561 agtacaaagg gatgtcgact tcgatttctg aagcctatta tattcttgaa gaagtgagca
    13621 agcaattatc agataccatt gaccaactgg attttgatgg tgggcgatta caggaaattg
    13681 aatttcgctt ggatacactc aatagcttaa cccgtaaata cggtggtaat gtgaatgatg
    13741 tgcttgacta ctacgataat attatcaaag aataccagct attaacagga gacgacttgt
    13801 cttcagggga tctagaatca gaattgaaag ccttagaaaa acaattggtt gctgcagcca
    13861 ctaacttgag tgaacgccgc catcaattgg cggagcaatt agaagctgag attaaagctg
    13921 aacttaagga actttatatg gaaaaagcag atttcaaagt tcactttacc acatcaaaat
    13981 tcaatcgtga tggtaatgaa agcttggaat tttacatctc tactaatcca ggcgaaggat
    14041 ttaagcccct tgtcaaggtg gcttctggag gagagttatc acgccttatg ttagccatca
    14101 aggctgctat ttctagaaaa gaagataaga cgagtattgt ttttgatgaa gtagatacag
    14161 gtgtttctgg tcgcgttgcg caagccattg cccaaaaaat ttataaaatt gggcgacatg
    14221 ggcaagttct cgctatttca cacttacctc aagttattgc tattgctgat tatcagtatt
    14281 ttatttctaa agaaagtaaa gaagagtcca ccgtttctaa ggtaaggtta ttaactcctg
    14341 aagaacgtgt ggaagaaatt gctagcatga ttgcaggaac agatatgaca caagctgctc
    14401 ttacgcaggc tcgtgaacta cttgccaaac attaagatta tgtttggtat ttgatatttt
    14461 cataattaga tgacattgat tttttaggtg ctaaaataat cattgtgcta gaccattttt
    14521 cctctgttaa tctggcttgg tttgccttga caaacctctg agaaatagta gaatagataa
    14581 gagaatttta agctgagggg atagaaatga ctagtaaaaa agcgtgttta tcaagcatca
    14641 ttgtgttagc aagtttaacg tgtggaaatg atactgttag tgccaatcat ctctcagcaa
    14701 ctggagataa gtttgatgat tgctcaacac ttgttgaaaa agatgtggcc cctaaagatg
    14761 aacttgagat gttagcatgg tcctcgtctc aaacaactga tgatgctgac agagactatg
    14821 aagattttct cgatgatgat tcttttaatg atgattcttt tatttctcaa aatgaaactg
    14881 ataagatgtt tgagaattta actgatgata ggttattaaa tgaattagat gaattagatg
    14941 aattagatga attagatgaa gaagatacaa ttgagccaga gcaaaatgta ataatgccta
    15001 gtgacgatga gctatttgat ttaactgatg ctgttgagac acgccttact gtttctagtg
    15061 ctccccattt agaggctgaa ttgccgaaac cacatttgag gagcctatca gatacagcac
    15121 tgcggtctgg tgaaattaga ggacatttag ataacaaact ggacactttg tctgtaacag
    15181 ctacaaagtt agcattaacg atggctcaaa aatttgattt gacaacgcat gtctattcta
    15241 taggtgaaag ctttagtgaa gtattagctg ctcattatga agacagaaaa gcagaatcag
    15301 ctttttctaa gaaaaagaga tttcaccttc ctattgctac tccagatgtt gttatagagg
    15361 agttaaggcg cctagtctct tctattggaa gttcaaaaga agatgtttca gttccttata
    15421 atcggaagct aggtatggca gttgcaaaaa gaaaaatagc cctgccacaa acgggagagg
    15481 agttctctta ttatccagct ttacttggtt taatgatatt aggattaacg ccgattatga
    15541 taccaaagaa gataaataat taggtaataa gttattgtag ttgtaaaaga cggggcaaat
    15601 gttcaagcgt ggaaaggttg acttttgcct cgttttgtat ctcgttattg ctaaggcatt
    15661 ttcttcgagc agctagtgct cgccatacct atctaccgag tgattattca atagcact
//
LOCUS       DAUCWU010000032        15168 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUCWU010000032 DAUCWU010000000
VERSION     DAUCWU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15168
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.032"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..756)
                     /locus_tag="VGY24_000945"
     CDS             complement(<1..756)
                     /locus_tag="VGY24_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ4283932.1"
                     /translation="MTKILTALDGRNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLS"
     gene            complement(1051..1767)
                     /locus_tag="VGY24_000946"
     CDS             complement(1051..1767)
                     /locus_tag="VGY24_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4283933.1"
                     /translation="MRLDKLLEVVTDGSRRQVKQLIKGQDVRIDHEIARSGRQNVDPG
                     LQLIEISGKRISHPKHSYFILNKPAGVVSARSDINHVTVLDQLSEKNRHPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVAKTYMVTVNGFLDADAPDFFAAGIKFPTGEQ
                     CRPAQLTILQASQEKSQARLVISEGKFHQVKKMFLTYGLKVISLERTSFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            complement(1871..2584)
                     /gene="nagB"
                     /locus_tag="VGY24_000947"
     CDS             complement(1871..2584)
                     /gene="nagB"
                     /locus_tag="VGY24_000947"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ4283934.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDV
                     EAEAKRYDQIIAEHPIDFQMLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIDDVPKQAISMGIASIMKSKMIVLLAFGEEKADAIKGMVSGPITEDLPASILQQH
                     ENVIVIVDEAAASKLNEVD"
     gene            2786..3814
                     /gene="queA"
                     /locus_tag="VGY24_000948"
     CDS             2786..3814
                     /gene="queA"
                     /locus_tag="VGY24_000948"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ4283935.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDIKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            3821..5041
                     /locus_tag="VGY24_000949"
     CDS             3821..5041
                     /locus_tag="VGY24_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ4283936.1"
                     /translation="MKLLGKWLFFLMFLTFCVNFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSALIQACYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(5155..5732)
                     /locus_tag="VGY24_000950"
                     /pseudo
     CDS             complement(5155..5732)
                     /locus_tag="VGY24_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S11"
     gene            complement(5729..5977)
                     /locus_tag="VGY24_000951"
     CDS             complement(5729..5977)
                     /locus_tag="VGY24_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283937.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGFGESQIAFSY
                     KDLNSGRTAAMNDHQPIIAGSTYKLPLNMLVVDKANYP"
     gene            complement(6006..6188)
                     /locus_tag="VGY24_000952"
     CDS             complement(6006..6188)
                     /locus_tag="VGY24_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283938.1"
                     /translation="MLRKKKTTIVGIILGMMIVTLLVGCLTGKKKGFPPKAGERQQTA
                     DLKMKKRLEKLFKNVT"
     gene            complement(6355..6960)
                     /locus_tag="VGY24_000953"
     CDS             complement(6355..6960)
                     /locus_tag="VGY24_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ4283939.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTSDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIVNWKKVSELYQAAK"
     gene            complement(7057..8097)
                     /locus_tag="VGY24_000954"
     CDS             complement(7057..8097)
                     /locus_tag="VGY24_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ4283940.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIDFDK
                     DDLAYSYFDMSEVAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            complement(8168..10411)
                     /locus_tag="VGY24_000955"
     CDS             complement(8168..10411)
                     /locus_tag="VGY24_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ4283941.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKVFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVVQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSIKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSDPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPNRETLARLEDRQITYYRTDTQGAIRLTGWTS
                     WHLETVR"
     gene            complement(10392..11054)
                     /locus_tag="VGY24_000956"
     CDS             complement(10392..11054)
                     /locus_tag="VGY24_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ4283942.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDNSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKRAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            complement(11254..11994)
                     /locus_tag="VGY24_000957"
     CDS             complement(11254..11994)
                     /locus_tag="VGY24_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ4283943.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKRLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            12034..12888
                     /locus_tag="VGY24_000958"
     CDS             12034..12888
                     /locus_tag="VGY24_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ4283944.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEGQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            12878..13156
                     /locus_tag="VGY24_000959"
     CDS             12878..13156
                     /locus_tag="VGY24_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ4283945.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(13180..>15168)
                     /locus_tag="VGY24_000960"
     CDS             complement(13180..>15168)
                     /locus_tag="VGY24_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ4283946.1"
                     /translation="HLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQGGVNQ
                     VSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWLIIPA
                     MIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFGIQR
                     FGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIF
                     ILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIE
                     LNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVTYPGA
                     NLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKG
                     TRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFK
                     YVKSLNLNDYKEQIKQLRDDVCFDFYQTNVVYLSNRMQDHMIDRSILYSILDKRPKRA
                     QVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQDLMES
                     GRLPKQEQEYTITPGRDVGDFRFVLIEERVTNARQLSNFERFIMQTKASIKHVTASPM
                     RWFGLQYSEVTLEVIPLILSDVLKLPIKELVPVEDSEA"
BASE COUNT         4794 a         3163 c         2720 g         4491 t
ORIGIN      
        1 tgataagtaa gccttttctt gtcgtcgtag ccatttcggg tgtctagcta aaagagctct
       61 ttgtattctt ttagataggt tcacagtcat ttttttctga taagaataaa ggatttcttg
      121 ctgatagggt aaccgaaaca tttccataat attatggtct aaagaaatgg tttttgttag
      181 gtaacttacc ttaccaaata agtcttcatg gattaaatat ttgagacgta gcagatttgc
      241 agctgcatcc agctcccaca aatgaaagcc caaactagat gaaaagtaaa gaaactgttt
      301 ttgcaaagca gttaagtgag tgttaagcca caattttctt cccagtaacc atcgtaccgg
      361 ataccccttt tcttgataag ctttggtgcg tttctttaga cgctcaactg gcaagggact
      421 gcactgaatt tctaaggcta acttatcatt cacccaaagg tctgctatct gttgcagctc
      481 aggaagatat ttttcaatac agacagcttc tgttcggact aaactggtgt aaagttttgc
      541 cttcaacgtc aaatgttcct cagattcatt ttctgcttgg aattgacaat gagctagttg
      601 tacatgggca aaatgaggtc gtctaattgt cccctgacgc aagcgaacag gagatttgca
      661 agctgggcaa cgaaaaggcg gtttcgttga aataggctga gttactagcg aaatgagctg
      721 attcctacca tctaaagcgg ttaatatttt tgtcacctcc tattagatct tccaccttat
      781 tattcgaaaa ctaagtacca aactttaagt agctttagtt tttttagcca tcaaaaaaca
      841 ctcccatttc caatttatga gagtttaaca caagggtttt tataacgctt caggttaagg
      901 agacagtggg aattgaatct ttttgcttac ctgattttgt aacaataaga tgactaaaca
      961 tagcaaaagt cggactcatc atgactataa tagtcaatgg acactttatt gattaagcaa
     1021 gaatagcttc tttttgaaag catgttttgc tcagtctaga tacgttttaa tgagttgcct
     1081 ttctgacgct gttagataac gatattctcc gctggctaaa tgaccgagtt ccagtccggc
     1141 aaaactagtt cgttcaagag aaatcacttt caagccatag gtcaaaaaca tttttttcac
     1201 ttgatgaaat ttaccttctg aaatgaccaa acgtgcttgt gatttctcct gactcgcctg
     1261 aaggattgtt aactgagctg gtcggcactg ctctcctgtt ggaaacttaa ttcctgctgc
     1321 gaaaaaatct ggagcatccg catctaagaa gccattgact gttaccatat aggtctttgc
     1381 aacatgatga ctaggatgta acattcgaaa acctaaaggc ccattatcag tgagtaaaac
     1441 caaaccttca gtatctctgt ccaatctgcc aactggatac aagtctgggt gtctgttttt
     1501 ctccgaaagt tgatccaaca ctgttacatg gttaatatcg cttcttgccg agacaacccc
     1561 tgctggttta ttaaggataa aataggaatg tttgggatga cttatccgtt ttccggaaat
     1621 ctcaatcagt tgtagcccgg ggtctacatt ttgtcgccca ctcctagcaa tctcgtgatc
     1681 aattctcacg tcttgtccct tgatcaactg tttcacttgc cgccgcgaac catctgtaac
     1741 tacctctaat aatttatcta aacgcatatt agtagtgtaa cataaattga tagcaacaaa
     1801 agaaaaagaa ggaaaaagac ttcacctcaa gaactcttct tcctcttcct tcttataaaa
     1861 ttattaatga ttagtcaact tcatttaatt tagatgctgc agcttcatcc acaatcacaa
     1921 tcacattctc atgttgttgg agaatactag ctggaagatc ttctgtgata ggaccagata
     1981 ccattccttt aatagcatct gctttttctt ctccaaaagc gagaagaaca atcattttgg
     2041 acttcatgat agaagcaatg cccatagaaa tagcttgttt tgggacatca tcaatacttg
     2101 taaagaaacg actattggct tcaatggttg attcttgaag atccaccaca tgcgtttctt
     2161 cttcaaaaga agtcccaggt tcgttaaaac caatgtgccc attacgtcca atcccaagca
     2221 tttgaaaatc aatcggatgc tctgcaatga tttggtcata tcgtttagct tctgcttcga
     2281 catcggttgc taaaccattg ggcaagtaat tctttttaaa aggcttcgca ttaaaaaggt
     2341 tttgacgcat aaaataatca taactctgat cgctctctac tgaaagaccg acatattcat
     2401 ccaagttaat acttgttaag tccgaaaaat ccaaaggact tttgaccatt tcttggtaaa
     2461 aactaattgg tgaacttcct gttgctaatc ctaacgtctt agctccctta gctaagctat
     2521 ctttcaataa ggtaaaagcg attttcccac cttcaatctg atcttggacc cgaataattt
     2581 tcatgtcatt ctccttttct aattggtata gacctttcgt gtttttatta tatggtatat
     2641 accaattttt gtcaagccta tcctttactt tctgatgaaa gattaaagat gacgggttag
     2701 acgacaagtg atataataga aaagatttat cttacatgaa agagttacta ggaagagatt
     2761 tactagtggc tttaaaggag tcccaatgaa tactaacgat tttgattttg agttaccaga
     2821 agaattgatt gcccaaaccc cactcgaaaa aagagacagt tcaaaactat tgattattga
     2881 tcaccgtcaa aaaactatgg ttgatagcca ttttgaccat atcattgatc aattaaatcc
     2941 aggggatgcc ctcgttatga ataacacccg tgtcttgcct gccagacttt atggagaaaa
     3001 acccgatact catggtcatg tggaattgct tttgcttaaa aatacacaag gagatcaatg
     3061 ggaagtttta gccaagccag ccaaacggct caaggttggt agccaagtca attttggaga
     3121 tgggcgtctc aaagccacca ttatcgacga attagaacat ggtggacgaa ttgttgaatt
     3181 cagctatgat gggattttct tagaagtttt agagagcctc ggcgaaatgc cattgcctcc
     3241 ttatattcac gaaaaattag aggatgctga gcgataccaa accgtttatg ctaaagaaaa
     3301 tggctctgcg gccgccccaa ctgctggtct ccacttcaca acagacttgc tcaaaaaaat
     3361 cgaagccaaa ggggtacatt tggtttattt gaccctacat gtagggcttg ggacttttag
     3421 acctgtttcc gtggataacc tcgatgagca tgatatgcat tcggaatttt attccctctc
     3481 agaagaagca gcgcaaaccc taagagacat caaacaagct ggcggtcgtg ttgtcgctgt
     3541 aggaaccacc tctattcgta cgttggaaac catcggaagt aagttccaag gggacattca
     3601 agctgattca ggttggacca atatttttat taaaccaggc taccaattca aggtagttga
     3661 tgctttttca actaatttcc atcttccaaa atcaaccctt gtgatgctag tatcagcctt
     3721 tgcagggcgt gactttgtcc ttgaagctta cagacacgcg gttgacgaaa agtatcgttt
     3781 ctttagcttt ggcgatgcca tgtttgtcaa ctaaaaacct atgaaattac ttggaaaatg
     3841 gctattcttc ttgatgtttc tgactttttg cgtcaatttt tggcagaaag actatcagcg
     3901 gattgccttt gaaaaagaac acaaacttta tcaagattta gtggcacctt tttaccaaaa
     3961 ctatcctaaa ttacgagaac tccagctttc agaagctatt aaattacgcc acgacttgct
     4021 tgattatgtt cgaaagctcg ataaggacgg atggtcctat aaagccatcc aaaaaggata
     4081 ccttgaacaa ctcgctgtta gaggaaactc ttatcatttc gaacaacttt atagtcgcat
     4141 tcgcctgata ggatcacctg actttcaaaa gctttggcaa caagaagaga tggcacagac
     4201 gcctcaagaa gctcaaaaac ggttacaact gctcttgacg tatttaaaga tgccagacga
     4261 gttgacagga caatcaaaac aaacacagca agtattggca caattaagtc caaagctatc
     4321 gccaacagat cctttttggg accaattatc tgccctgatt caagcttgtt atgttaatct
     4381 cgaacacatt ccttatagcc gttttaatcg gaaaattcat caactccgtt acctcatatc
     4441 aacccagcaa acggagtggg ttcgctcaca ttatgggaat aaagggaaaa ccgatgcgga
     4501 tgccttagcc aaatatttgg ctactctaga agatgacgat tatgctcttt atgaatcttc
     4561 tagataccat aataaggttg ctagtatttt agatgctaat ggcaatcatc aggcagtcta
     4621 tacagacaat attcctcaag ccaactataa gattttaatt cattttcact cagagtttat
     4681 tttgtcagaa tctggacaat ttttagtagc actagatcct gataacctaa cgcgaaatag
     4741 tattgtcaat ggttcttctt tcaattatgg caaccaaaac gatgacttgc accgtctgct
     4801 tgatattgac cctattctgc tttttgatcc ggcttttatt gaaaaagcaa ttaatagtcc
     4861 tgatgcaact tttctagttc ccgatttaga acagcaaagg gataagcaca atcccattta
     4921 cagcagaaat ggcaaatcat ctaagcagct aacacgcgct gccgttaaaa aattcaaaaa
     4981 attgcttcac cattatcaag aaacaagagg tgacaataag acctcaaaaa cacagcatta
     5041 atctcaaaaa gcttgacctg aagaaacagg tcaagctttt ttaatgagca acaacttatt
     5101 ttagtgactg tcatcagtaa gtatgtctac tatcagacta atgtcaactg agtattagat
     5161 catgttgtga ttgactctat gccactcatt aattacatat gttagctgaa caagctgcac
     5221 ataaccaagg ccattgactt cctctgatgc ttgtgttgct ccacccaaac cagatgaata
     5281 aagagcaatc aaataaggaa actcttcgca aacaatggca cctacattta aggcttctct
     5341 gacataaccc ggtttttgat aaacctttac atgaggcaaa taagtcttgt aatacaaatc
     5401 agggaaagat tcaccaatgt agtacaagat atccttatat ttatcctgat gctgccacaa
     5461 gtaatccaaa acttgcaaat agtaagcggt ggtgatttta ttgccattac gatcaatggt
     5521 cgtaatagcc ccggatacct tcccgtagcg gtcaaataat tggtaagcct tattcatgcc
     5581 acccaatcgt tctgctagag cataagctgg cgtgatttct gagtatcttg catatccgga
     5641 atagacatgg caccattgaa ttgagcaaca taggcatgat gttcctgatc atattcataa
     5701 tcagtttctg taatgtcaaa acgctctgtc atggataatt tgccttatct accaccagca
     5761 tgtttaatgg cagcttatag gtgcttcctg caattattgg ctgatggtca ttcatggcag
     5821 cagtcctgcc tgaattgagg tctttgtagg aaaaggcaat ttgggattca ccaaagccaa
     5881 attcctttaa atacaccttg accgtattga ccaaactcaa atggttgtca tcgtaataga
     5941 gccctaaggc atccatttgc gctaaatctg catccataac agcttgctta gcctctttag
     6001 atttttcagg taacgttttt gaacagtttt tctaatcttt ttttcatttt taagtcagcc
     6061 gtttgctgcc gctcacctgc tttaggagga aagcctttct ttttaccagt caaacagccg
     6121 acaagtaagg tcactatcat cattcctaaa ataatcccta caatggtagt tttcttcttt
     6181 cttaacacct aaaatctcct atccattcta ttctagctaa atttagctaa aaatcaagga
     6241 attacattaa aaagacaaac tatttttatc ttttctaaaa aatgatgtcc ccacactgtt
     6301 attcaaaaaa gcacttaaaa aaccaatctc tttttgagat tggaaaccct tttcttattt
     6361 agcggcttgg taaagttctg atactttttt ccaattaacg atttcaaaga aagctttgat
     6421 ataatttgga cgtacattac gataattcaa gtagtaagca tgctcccaca catcaagcgc
     6481 tagaattggt tttttgcctt ctgaaattgg agtatcttgg ttagcggttg atgtgatttc
     6541 gagttgtcct tctttattaa caactaacca tgcccaacct gaaccaaaac gtccagtagc
     6601 agctgctgta aattgttctt taaaggcgtc aaatgaccca aaagcatcat caatggcttg
     6661 tgcaacatca gaagtgacgt cttgtttttc aggtgataat agttcccaga aaagcgcatg
     6721 gtttaggtgt ccgccaccat tattaattaa ggcttgacga atatcttctg gaatcttagt
     6781 gacatctgcc aatagttcct ccagattttc tccgatctct gggtgttttt ctaaagccgc
     6841 attggtatta gcgacataag tggcatggtg tttatcatga tgaagagtca ttgtctccgc
     6901 atcaaactgt ggttcaagag catcatacgc gtatggaagt tctggtaaaa taatagccat
     6961 tttcctgtcc tcttttcctt tatgatacgt ctatcttatc gcaaaaaata agagctttca
     7021 actaatttga ttatctcttg gaaagcggtc caacgcctat ttttgagagt gagtcatgat
     7081 ttttaagaga gcaatatcaa ctagataact cttctcataa agtcctgttt ttatctggta
     7141 atctgtctca atcaaggttt tcaccgctcc tgttagaaag gcaagagata aggtcctaga
     7201 atcctttaac gcatacttga cctggtaagg attaacccgc cgaccaagta tctctgacaa
     7261 actggttaca agctgctgtt ctgattttcc ttctttagca agaatggcaa gctgtaaata
     7321 taagcgaaat tgccccaaca taatagcaat taatttaata tcatcttccc ctgataagcg
     7381 taaatcatga atcaaatctc tagccacatc aactttacct cctaggacaa gtctggttaa
     7441 gtcaaaaata ttatcttgta aacttttagg aatggcttgc tcaatatcag ttaggctaat
     7501 atttcccgtt tttttatagg cttttaaaaa agccatgttt ttcatgattt gactaaaatc
     7561 atcgtttgat tttaaaagca gttgttcaaa agcgccactc tcaaatccca aacccagttg
     7621 atgactgtat ttttgaaaat aagttcttag ctctgcttct ttcagagggt tggcttctaa
     7681 aacaagggca tcacgtttca aaagcttaac aagccgtctc ttactatcca atttacctgg
     7741 agcaaagata attagtcgag tagtctctaa gggattttct aaataggctt caaaggcctt
     7801 taggtctttt tcttttaaga aacttttttt attggtcgtg atatctaaca aatggtcaaa
     7861 aatgaccacc ttctgctcag caaagaaggg taggctcact agatccattt ctgcatcctg
     7921 ataagcgacc tcagacatat caaagtaaga ataggccaaa tcatccttat caaaatcaat
     7981 ctgctccatt aagcgggatt tcaactggct atactgacca atgtcatctc ctgtgacaag
     8041 ggttataaga cccaaatttt ctttactcag tttttcaatc ttttctatcg caatcatgct
     8101 tttattatat catctttctc ctaaaaggaa agcgactgct tgtttcttcg tcaggatgga
     8161 attttaacta acggaccgtt tcaagatgcc agcttgtcca accagtcaaa cgaatagctc
     8221 cttgcgtatc cgttcggtaa taagtaattt gtctgtcttc caaacgggct aaggtttctc
     8281 tgttaggatg ctgataacgg ttattttttc cagcagaaat aaaggccact ttaggttgaa
     8341 tatggtctaa aaaagctgca ctagaagagg tatttgatcc atggtgacct gcttttaaat
     8401 aatctactct tagttgagga taacgtttta tgatctcgtt ttccccctct ttttccaagt
     8461 cacctgtgaa aagaaaagtt cgatttaaga gcctgccata aagtaccaaa gaatcgttat
     8521 ttttcccgtc ccctaactgc caagggtaga gtacctgtaa gacacttccc ataataggca
     8581 actggtctcc agctgctaaa accctaacgt gacattttaa acgccttagc cgcctaacaa
     8641 agcttggatc gcttaaactg ccttgactag tcaaaatctc ctttatccga atagctttgg
     8701 caaccacttc gatatccccc atatgatcag tatctgcgtg agttactaat agttggtcaa
     8761 ttgtgtgaat acctctactt tttaagtaag gaattaaggt tttctgagca ttagctacgt
     8821 gatgtcctcg tcgccagtgc tcttttgaag aaaagaaagg gcgcccacct acatctatta
     8881 agagattttt ccctgtccac tccctcacca aaatactatc tccctggcca atgtcgataa
     8941 aggtcacttc attggtcaaa ggatatttga tagacaaaag cgtcaacgct ataaccaaac
     9001 cgcaagtgat aactcgttgt cgcacctgcc gataatcata aaaaatagcg aagctaatca
     9061 ccaagataag caagtgccaa ctggtcgggc ttccaaaaac aagggaacta ttgaaagtat
     9121 ttccgagaaa ttgaacgact ttttctaata aaataaaaag atggttgcat attgagacag
     9181 tcaccaaagg ggatagacaa aaaaccagac aaagaagggg caaaataaaa aggtcaaaga
     9241 gataagacaa gagccctgtt aagaccattg acatggggtt aaaactggaa aaataaaaga
     9301 gcaaaaatgg taatattcct agggaaatgg tcaagacttg taccaactgt cttttagctc
     9361 ctgataattc ttcaacagta accactgtta acaaaaaagc atagctaaac gttaatacgc
     9421 caccaacagt cattaagaaa tgagcatccc ataaaaagac aagtaaaaaa gtacaagcca
     9481 gattgtctaa tccttttatt cctaaatgac gcaactgcga ctgcacgagg cttcgaataa
     9541 cagaaatact ataacctgtc aacgcggcat aaaagcaagc aaaaggaagc tctatccatt
     9601 ttatccattc caaaggaact gcgagtaata gcaacaccct tctaaaacaa gtaagaaaga
     9661 agccaacctg catccctgat aaagcaaaga gatgaatgat acccaactga ctataataat
     9721 ctgtcatctc accaaacgac ttatctaaat aaccaaaaag gaggcccgtc aggtagtgag
     9781 acataggtct tggaaaatgc tgctggcaat gaacaatggc tcgtcgtctt aaagaggaca
     9841 aatagtcaca tattgactcg ggtgaaatca cttcaagctg ttctatttgc tctaccttcc
     9901 ctatcctgta aattccttga taagccaaaa aagtctgata attaaagact ttaaaatttc
     9961 gaacttcctc agctttttcc aatgtcactt ttgcgtgcat cactaaccaa cggtgctctt
    10021 tcttgaacaa ttgggcttct gcttggcttt tcaaacggta aaataactga taagaatgtt
    10081 tcccatgacg tccaaggaca gctaattgat caccattaat ccgaatacta tcaggaacta
    10141 atgctacaga ggttatttgt actggctgaa tttccagttt ttgaaccaac tgctgtttct
    10201 gatacagcaa aaacaaagca cagaaactaa ggatggcaaa gacaccgaga aattcttttt
    10261 tagacaaacg cttgacaagt aagcaaatca aactaaggct aaggagaaac gttaaccagc
    10321 tagggctatg gatttgataa aagagaacca agattaaaaa agcaaattga atctttgaca
    10381 ggggcactag cttagtccaa gaaaatgtca tcttttaatt tctcgagcgt tttttcgcca
    10441 atccccgaaa cctgacggag atcttctaac gctttaaaac cacctaactt atctctcata
    10501 tcaataatat cctgagctct tttagcacca atacctggga tatgttgtag ttcttctaag
    10561 ctggccttat taatatgaac ctttgactga tccttgctgg cagtttcttt tttaccagaa
    10621 actaatgatc ttggtagcac agaaatctct tctccttgtt taggtacata aacaacttgc
    10681 tcatcagtta gtttttctgc aaaattaata gcacgtttat cagcttctga agttaggcct
    10741 ccagctagtt caatgacatc tcgcacacga ctgctagcag ttagcttata aactccctct
    10801 ttttgtacgg cgccttttaa atccactaaa atactgttat cttcttgaat ttcctttttt
    10861 tcttcactaa cctgctgttt cacaggcgat atttctctca aagcaggaac ggctgccttg
    10921 ctctgcctat ttccccagaa caaaaaactg aagcttagta ttagaaaaag tcctaataaa
    10981 agactaatta ggtatggcag aaaataccgc tctctcaaaa acgattttcc tttaattaat
    11041 aattcgtcta acattttttt acctcatctt tttattcgta aatgatatcc aaaagtatag
    11101 tagtattgtc attttttgtg cttaaaagag ttaagggtat catcgaatat cttttttaga
    11161 aatgaaaaaa gtgattaagc attagcggct tctttcaata gaagactaat ctcaatcact
    11221 tcttatttca ggcacatgtt agccctcatg ttcttaatgg cgatgtttat ctggattcca
    11281 aacaaaacta gcaatatagg aaaataacat ggttaataat aataccacca ccaaaaccaa
    11341 tcctaatggt aaacggtaga gataagttaa agggttcctt gctttgcctg gttggaaagg
    11401 tgccaactcg tcatccatcc gatcaaactc tgcctgaata cggtttgcca cctcagcaat
    11461 cccttcatca ttcatgcgtt taatatctga gacgtcaatg ggattcccaa aagtcatgtc
    11521 aacccgttct cccgctagta atctcttgac cgacataggc ccctgataag ctgctggcat
    11581 aatcttgacc ttagccaatt tagcaatcac agcgacccct cctttgacct cttgcgaatg
    11641 cctacttcca cttggaaaca taagaagaga acgattgctc tttttgagca tattaacagg
    11701 gtaacgaata gcatctggac tcggtttgtc acgatcaata ggaaaagcac cacacatttt
    11761 aatccaccaa gcaaagagac gatttgcaaa caactctttt ttagccataa aaataaactg
    11821 cttaggacgt gctgcaaatg ccatgtaaac tggatcccaa aaggttcgat ggggtgctac
    11881 cagaatataa ttctcagaag catctaacat cttttcttca tgatggtagt gggcattgcc
    11941 attgacaacc cataataaaa agactactaa accgcgtaag taagcgtaaa acacaggctt
    12001 ctcctttaat agaataatct tacttattgt atcatgaaag ctgacttttc gaaagtcaaa
    12061 atcactcttc aaacattcac ctcaagccta ttttatgtta aaataacagg tatgataaaa
    12121 gcaatcttaa aagaaggcga acgtattgat caactctttt caagtgatgt tggcattatt
    12181 caaaacaagg atgtctttag ttactctatt gatagcgtcc tcttatcccg cttccccaag
    12241 atgccttcca aagggttaat tgtggatttg tgctcaggaa atggggcggt tggcttattt
    12301 gctagcacta gaaccaaggc agccatcgtt gaggtcgaat tacaagaacg gcttgctgat
    12361 atgggacaac gctctatcca gctcaatcaa ctggaaggcc aagtaaccat gatttgtgat
    12421 gatttaaaaa acctcctaaa ccatgtgcca cgttcaggag ttgatttgat gctctgcaat
    12481 cccccttact ttaaaagtca cgaaagttct aagaagaacg tctctgaaca ttatttactg
    12541 gctcgtcatg aagtgaccac taatttagaa gagatttgtc aggtagcccg tcatgccctt
    12601 aaatcaaatg gtcgtctggc aatggttcat cgaccagatc gattcttgga aattatagat
    12661 agccttagag ctaatggtct tgcccctaaa agggtacaat ttgtctatcc taaattaggt
    12721 aaatcagcta atatgttact tattgaagca atcaaagacg gttctattga agggatgact
    12781 attttacctc ctttagttgt ccacaaggaa aacggagaat acactgatca tatttttgag
    12841 atttattttg gtgctgcttc caaaggaaaa cctaatcatg accactaaaa aagcttatat
    12901 gtatgtgtta gaatgtgttg ataaaaccct ctacactggt tatacaactg atttgaaaaa
    12961 gcgtttagct acacataacg caggaaaggg ggctaaatat actcgctacc ggttacctgt
    13021 ttctttactt tattacgaag tgtttgatag caaagaggct gcgatgagcg ctgaagcact
    13081 ctttaaaaaa cgaaaaacaa gaagccagaa attggcctat attgctactc atcaaaaaga
    13141 aaagaaaaat cattgagact tatcatctca atgatttttt tatgcttcac tgtcttccac
    13201 aggcaccaat tctttgattg gtaacttcaa gacatctgat agaatcaagg gaatcacttc
    13261 aagggttact tcggaatatt gaagcccaaa ccaacgcatt ggactagcgg taacatgttt
    13321 aatgcttgcc ttagtctgca taataaagcg ttcaaagtta ctcaactgac gtgcattggt
    13381 gacccgttct tctattaaaa caaagcgaaa atcaccaaca tcacgaccag gtgtgatggt
    13441 atattcctgt tcttgtttgg gtaaacggcc actttccatc aaatcctgaa caatcgtgcg
    13501 caaatagcgt ggcacagtct gtggcattct aaaaccaaga taaaggttga ctcgaaccat
    13561 ataatccgtt cccatcatat ctaccttgta cttagctgta taaggttcat cagttacctg
    13621 gacatttaca aaccaataaa cttgagcgcg ttttggccgc ttgtctaaaa tggagtacaa
    13681 aatagaccgg tcaatcatat gatcctgcat acgattggat aagtagacaa catttgtttg
    13741 gtagaagtca aaacaaacgt cgtcacgaag ttgtttaatc tgctccttat aatcattcaa
    13801 attaagagac ttcacatact tgaaaacaat ccgtgtgccc gcatgccaga taaacatgac
    13861 aaagacaatt gcaagtgcca agataacaac cgcatagcca ccgtgcataa atttgatagc
    13921 tgaagccaag aagaaaatga attctacaag ggcaaagaaa gccatcgcta aatgagccaa
    13981 gatgggtctc gttcctttct tgattaagta atactttaag aggattgttg tcatcaacat
    14041 ggtgatggta atagcaagac cataagctgc ctccatgtgg gctgatgttc taaaagccaa
    14101 aacagtacaa gaggtcactg caaataaaat ccagttaatg acaggaatat acaattgccc
    14161 caaatttgcc cctgggtaag tcacacgaaa caaagggaaa attttcaaac gcatggcctc
    14221 tgaaaccaag gtaaaggagc ctgaaataag agcctgtgag gcaataatag ctgccaaggt
    14281 tgccaaacta acaaggtaaa ctcttaattg tgatggcaca ctagcaaaga aagggtttag
    14341 ttcaatacct gagtgtttat tcgctaaaat ccacgcagcc tggccacagt aagacaacac
    14401 aatacacatc ttaacaaagg gccagctcac ataaatattt cctcgaccaa catgtcctaa
    14461 atccgaataa agagcttcag ctcctgttgt cgcaagaaaa atggacccca agataaaaat
    14521 cccacgatgg ttttcaggac taaacagcaa atgcagagca tagtaaggat tgatagcctt
    14581 gaaaatttcc agatgcccca acgtattaaa aaaaccacta acacctagaa aactaaacca
    14641 gataaacata actggtccaa aaattttacc aataaaacct gttccaaacc gctgaatacc
    14701 aaacaagact attaaaatca ctagagttgt gataatgaca ttggtttgat tttggtagat
    14761 gtgactcaga ccaggaacgg ctttcaagcc ctcaatagct gaagtaactg ttaccgcagg
    14821 tgtaagagcc ccatcagaca aaagtgttgc acctccaatc atcgccggaa taatgagcca
    14881 cggtgacatt ttacgtacca aggtgaataa agaaaaaatc ccaccttcat ggtggttatc
    14941 cgcttttaga gcaatcaaaa cgtacttgat tgttgtgatt aaggtcagtg tccaaataat
    15001 taaagagatt gatcctaaaa taaaggattc agaaacctga ttaacccctc cttgattttc
    15061 aactagagac tgcatcgtat aaagggggct tgttccaata tctccataaa caatacctaa
    15121 agcaataata aaacctgctt ttgaagcttt gtcaaaggca gtaagatg
//
LOCUS       DAUCWU010000033        14862 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUCWU010000033 DAUCWU010000000
VERSION     DAUCWU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14862
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.033"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            177..869
                     /gene="pyrF"
                     /locus_tag="VGY24_000961"
     CDS             177..869
                     /gene="pyrF"
                     /locus_tag="VGY24_000961"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ4283947.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            927..1556
                     /locus_tag="VGY24_000962"
     CDS             927..1556
                     /locus_tag="VGY24_000962"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ4283948.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            1753..3207
                     /locus_tag="VGY24_000963"
     CDS             1753..3207
                     /locus_tag="VGY24_000963"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ4283949.1"
                     /translation="MTYQDATAMVIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATNTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSLVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            3325..4188
                     /locus_tag="VGY24_000964"
     CDS             3325..4188
                     /locus_tag="VGY24_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4283950.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     NVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            4217..4867
                     /locus_tag="VGY24_000965"
     CDS             4217..4867
                     /locus_tag="VGY24_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4283951.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWLIAFLLERYQEFLENKLALV"
     gene            5000..5653
                     /locus_tag="VGY24_000966"
     CDS             5000..5653
                     /locus_tag="VGY24_000966"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ4283952.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLNEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKSFITNPKHHIIESSHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            5785..7053
                     /locus_tag="VGY24_000967"
     CDS             5785..7053
                     /locus_tag="VGY24_000967"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ4283953.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVEVVAFAQRLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYSN"
     gene            complement(7111..7752)
                     /gene="plsY"
                     /locus_tag="VGY24_000968"
     CDS             complement(7111..7752)
                     /gene="plsY"
                     /locus_tag="VGY24_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ4283954.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            7887..9836
                     /gene="parE"
                     /locus_tag="VGY24_000969"
     CDS             7887..9836
                     /gene="parE"
                     /locus_tag="VGY24_000969"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ4283955.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGAVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDLRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            9903..10721
                     /locus_tag="VGY24_000970"
     CDS             9903..10721
                     /locus_tag="VGY24_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEQ4283956.1"
                     /translation="MKLVIWQNTYSLQWDGTYHFALESYPMIQDWELEKIAAFCHYER
                     MNHREPQIICKDQVIVTKINQYLKHNNRKPPFTPSHKKVASTYDVSGKAVYGDWLSHT
                     CTVETAIAVFKSGKLLSAVKAFNRPAEELVKDSRNAAGDPADYFNYVMLGWSNTTSGY
                     RLAMERLIGHLPNDRELNELFIPGVSFHFSYEEVLAQEHYLFDGYHPAKVKNHLSLDA
                     LKACIIPLDQASLFEAIIPEVLKARCFYLPYHQEGLIEWTDSVYRFLVNLEMAD"
     gene            10919..13378
                     /gene="parC"
                     /locus_tag="VGY24_000971"
     CDS             10919..13378
                     /gene="parC"
                     /locus_tag="VGY24_000971"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ4283957.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            13456..14523
                     /locus_tag="VGY24_000972"
     CDS             13456..14523
                     /locus_tag="VGY24_000972"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ4283958.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAVPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITENNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            14587..14817
                     /locus_tag="VGY24_000973"
     CDS             14587..14817
                     /locus_tag="VGY24_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ4283959.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
BASE COUNT         4720 a         2676 c         3140 g         4326 t
ORIGIN      
        1 ttaaaaaagc tataatagtt taaatttaga cacctttaag tgagtccaga gaggcaaaca
       61 aggtatacaa gataaaaaaa gggaattccc ttttagtttg ctgtagcctc gcttctttgg
      121 tagcgaggct ttttggtgct taatgagata ttgacacatt atagaaagga taagttatga
      181 aagaagaacg cccaattatt gctttagact tttcatcatt tgaggagact aaagcatttt
      241 tagacttatt tccagcagaa gaaaagctat acgtgaaaat tggtatggaa ttatattatg
      301 cgcaaggacc agatattgtt cggtatatca aatcgttagg gcataatgta tttttggatt
      361 taaaattaca tgatattcca aatactgttc gtgctgctat ggcagttctt aaagaattag
      421 atattgatat ggcaactgtt catgcagctg gtggtgtaga gatgctaaaa gcagctcgag
      481 aaggactagg tcaaggccct accttaattg cggtaacgca gctaacttca accagtgaag
      541 accaaatgcg tggtgatcaa aatattcaaa cgagtctttt agagtctgtt ttgcattaca
      601 gcaagggggc agctaaagct cagctagatg gggtggtttg ttcggcacaa gaagtagaag
      661 ctatcaaggc ggtgacacct acgggattta cttgcttaac tcctggcatt cgtcctaaag
      721 gcagtaatat tggcgatcaa aaacgagtga tgactccaaa ccaagcaaga cgcattggaa
      781 gtgattatat tgtggtaggg cgaccaatta cccaggcaaa agacccagtg gctgcttacc
      841 aagccataaa agctgagtgg gcaggctaac agataagatt aggaaaccaa cacttgtctg
      901 ttaaagaaga aaaaggagaa taagaaatga cactagcaag ccaaattgca actcaattac
      961 ttgatattaa agctgtttat ttaaagcctg aagatccttt tacgtgggca tctggcatta
     1021 aatcacctat ttacactgat aatcgtgtca ccctttctta tcctaagact cgtgatttaa
     1081 ttgagaatgg gtttgtggaa actatcaggg ctcatttccc agaagttgag gtcatcgctg
     1141 gtacagctac agcaggtatt cctcacggtg ccattatagc agataaaatg actcttcctt
     1201 ttgcttatat tcgcagtaag ccaaaagatc atggagcagg caaccagatt gaggggcgtg
     1261 tgcttaaagg ccaaaaaatg gttatcattg aagatttgat ttcaacaggt ggttcggtac
     1321 tagatgcagc cgcagcagcc agtcgtgagg gagctgacgt tctgggagtt gttgcgattt
     1381 tcacttatga gttaccaaaa gctagtcaga attttaaaga agctggtatc aagttgatta
     1441 cgctttctaa ttatactgag ctaattgcag ttgcaaagtt gcaaggctat attactaatg
     1501 atggtttaca tttacttaaa aaatttaaag aagaccaggt aaactggcaa caataaggaa
     1561 ctatcagcca ggtttatttt taatgaaaaa atctctttaa ccccaattca aaatgttatg
     1621 ttttgattgt tttgtctaca ttccttacac atcacattct aatttctaac cttttaatgg
     1681 tggttttctt ttatttgtgt tttaataaaa aagaatgttt taggattcat atcttagaaa
     1741 ggaagagtag ctatgactta tcaagatgcg acggctatgg ttattgctgt tcagaccggt
     1801 cagaccacac cactagaatt agtaacccag gctatttata aggctaaaaa gttgaaccct
     1861 accttaaatg ctattacctc agaacgcttt gaagcagccc tagaagaagc caaacaaagg
     1921 gatttttcag gtaagccttt tgcaggagtt cccatttttt tgaaagattt gggacaagaa
     1981 ttaaaaggtc actcatcaac ctcaggttcc agattgttta aagagtatca agcgactaac
     2041 acggatttat ttgtgaaaag attagaagct ttaggcttta ttattttagg gcgctccaac
     2101 acgcctgaat ttggctttaa aaatattagc gatagcagtt tgcacggacc agtgaattta
     2161 ccaaaggata atacacgaaa tgctggtggc tcatctgggg gagctgctgc tctagtgtca
     2221 tcaggaatta gtgcactagc aactgctagt gatggtggtg gttctattcg aattccagcc
     2281 tcgtttaatg gcttaattgg gctcaaacct agccgtgggc gcatgcctgt cggacctggt
     2341 tcttatcgaa gctggcaagg agcctcagtt cattttgcct tgaccaaatc cgttagagat
     2401 actagaaatc tactctacta tttacagatg gaacaaatgg aaagcccctt ccccctagcc
     2461 accttaacaa aagatagtat ttatcaatcg ctacaacgtc ccttgacaat tgctttttat
     2521 caacggttaa gtgacggtag cccagtatct ttagatactg caaaagcttt aagacaagca
     2581 gtaacttggc taagagaaca agggcatcag ctagttgaat tggaagagtt tcctgtaaat
     2641 atgacagaag tgattcgtca ttattatatt atgaatagtg tggaaacagc tgctatgttt
     2701 gctgatatcg aagacacttt tggacgtccg atgaccaaag acgatatgga aaccatgacc
     2761 tgggctattt accaatcagg taaagacatc ccagcttggc gttactcact ggtccttcaa
     2821 aaatgggata cgtacagtgc aaccatggct agttttcatg agacttatga cctgttgttg
     2881 acgttcacaa ccaacacacc agctcctaag cacggagaac ttgtcccaga ttcgaaactc
     2941 atggctaact tggctcaagc tgaaattttc agcagtgaag agcaattcaa tttggtcgaa
     3001 accatgtttg gtaagagtct ggctattaat ccttacacag ctctgcctaa tctaactggc
     3061 cagccagcta ttagtttacc tacttacgaa actaaggaag gtctcccaat gggcattcaa
     3121 ctaattgcag ccaaaggtag agaggaccta ttattaggaa ttgcagaaca gtttgaggca
     3181 gcaggattat tgaagattcc tcaataataa tcttataaaa cagtttaact attatagatt
     3241 aactttttaa agagattgag cgctcactga taacacaact taatcaaaat gttatactat
     3301 tcttatctta ataaaggagt tactatgaag aaatcagtta ctttactgtc tatcggactt
     3361 gccagccttt tattggcagc atgtgcgcca cataaatctc aaaaatcaag ttgggacact
     3421 atcaaagaaa aaggggtgtt aaaagtagct accccaggaa cctatcagcc aacttctttt
     3481 tacaacgata ataatgagtt agtaggttac gaagtagata tggtcaaaga aatcggtaaa
     3541 cgacttaaca ttaaagtcaa gtttgttgaa acaggatttg accaagcctt tacctcagtt
     3601 gatagtggtc gagtggatat ttctttgaac aactttgaca ttaccccaaa acgtcagaaa
     3661 aaatacaata tctctacgcc ttataaatac ggggtaggag gcatgattgt ccgtgctgat
     3721 ggcagctcaa acatggctaa aaaagatctt agcgactgga aaggaaaaaa agcagccggc
     3781 gcttctggaa cagaatacat gaaagtagcg caaaaacagg gagccgaact ggtgacctac
     3841 gataatgtta caggagatgt ttatctaaac gatgtggcta acggtcgaac agatttcatc
     3901 ccaaatgatt acccagctca aaaattattt gtggattaca tgttatctca aaatccaaat
     3961 ctaaatgtca aaatgagcaa tgttcagtac aacccaaccg aacaaggaat tgttatgaac
     4021 aaaaaagacg atagcctcaa gaaaaaaata gatgctgtta ttaaagacat gataaaagat
     4081 ggtagcttga agaaaatctc tgaaacctac tatgctggtc aagatttgac aaaacctttt
     4141 ggcaaagaca aaaaaattcc cgtcattgat acgaaagacg ttaactaata cttgatgtta
     4201 aaggagtcct aaccacatga ttaatatccc attaatgaaa gatagtttag gatttgtgtt
     4261 atcgggatta ccttataccc ttggtatttc gttgctcagt ttttttacgg gtctgttttt
     4321 aggtttaggc ctagcccttc tgggaaggtc acgtcagccg ttgattcatt atcttgttag
     4381 agcgtacatc tctattatgc gaggtgtgcc gatgatcgtg gtgctctttg ttctgtattt
     4441 cgggttgcct tattatggtt tagaattgcc agctttactt tgcgcttatc ttggtttttc
     4501 tatggttagc gctgcctata tttctgaagt ttttcgctct tctatagaag ctattgacaa
     4561 gggacagtgg gaagcagcta aggctttagg gttgccatat gctcttatgg ttaagaaaat
     4621 tattcttcct caagcttttc gaattgcagt tcctcctcta ggcaatgtca ttattgatat
     4681 ggttaaaagt tcgtcgctag ctgccatgat taccgtacca gatatttttc aaaatgccaa
     4741 aattattggc ggtagagagt gggattacat gtccatgtat attttagtag cctttatcta
     4801 ttggcttatt gcttttttgt tagaacgtta ccaagaattc ttagaaaata agctagcact
     4861 tgtttaatat cctgtaaaaa acgaatatta accctggaaa tatcatattt tcagggtctt
     4921 tctatttaaa atgtggtaaa ctagatagac aaggacatcg tatttaactt agaaactgac
     4981 gtgattagaa aggaatctta tggctcattc aatttggcat gagaaaatca aatcattttt
     5041 gccggagcat tactacggtc gcatcaatca ctttttaaat gaagcctatg cttccggtct
     5101 tgtctatcct ccacgagaaa atgtctttaa agccttacaa gtcactcctt tggaagaaac
     5161 caaagtgttg attttaggac aagaccctta tcatggcccc aaacaggccc agggcttatc
     5221 attttcagtt ccagaagaga tttctgctcc gccatccctt attaatattt taaaagaatt
     5281 agcagatgac attggtcctc gtgaccatca tgatttaagc acttgggcta gtcaaggggt
     5341 tctgcttttg aatgcttgct taacagtgcc agcaggccag gctaatgggc atgcaggctt
     5401 aatatgggag ccatttacag acgctgttat taaagtgctg aatgagaaag acagccctgt
     5461 agttttcatt ttgtggggag cttatgcaag gaagaaaaaa tccttcatta ctaatccaaa
     5521 gcaccacatc atcgagagct ctcatccaag ccctttgtca tcttatcgtg gcttttttgg
     5581 cagcaaaccc ttttcaagaa ccaacgctat tttagaaaaa gagggcatga ctggcgtaga
     5641 ttggctaaaa taagccagcc atttcaacta aatagcttaa gctagacatt ttgatacctt
     5701 taataagtga aatgagcgca gtagattttt cctattaaag aacgtaaggt cttatggtta
     5761 aaacagataa ataaaggaga aaacatgata ctcatcaaaa atggacgcgt tatggacccg
     5821 aaatcgcaga gagatcaagt ggcagatgtc ttaattgatg gtaaacagat tgtaaagata
     5881 gcttcagcta ttgaatgcca agaagctcaa gtgattgatg ctagtggtct tattgttgct
     5941 ccgggtttgg tcgatattca tgtgcatttt agagaacccg gtcaaacgca caaagaagat
     6001 attcacacgg gagccttggc agcagcagca ggtggtgtga ctacggttgt catgatggct
     6061 aataccaatc ctgttatttc agatgtcgaa actttacagg aagtgctagc tagcgcagct
     6121 aaagaaaaga ttcatattta caccaatgcc agtgtcactc aagcctttaa tggaaaagac
     6181 gtgactgatt ttaaagcttt gttggaagca ggagcagtaa gcttttcaga tgatggtatt
     6241 cccttagaaa gttctaaagt tttaaaagaa gcctttgatt tggctaatgc caaccaaact
     6301 ttcatttctt tgcatgagga agatcctcag ttaaatgggg ttcttggttt taatgaaggt
     6361 attgctgagg agcattttca tttttgtggg gcaactggtg tagcagaata tagtatgatt
     6421 gcaagagatg tcatgattgc ttatgacagg caagctcatg ttcatatcca acatttatct
     6481 aaagcagaat cggttgaagt agtggctttt gctcagcggt tgggtgctaa ggtaactgct
     6541 gaagtatccc cacaacattt ttcaacaact gaagatctct tattgaccgc aggtaccagc
     6601 gcaaagatga atcctcccct tcgtacacaa agagatcgtt tagctgtcat agaaggcttg
     6661 aaatcgggtg ttatcacagt cattgccact gatcatgctc ctcatcacaa ggatgaaaaa
     6721 gctgttgatg atatgactaa agccccatct ggtatgacag gacttgaaac ttctttgtca
     6781 ttaggtttga cgcatttggt tgaaccaggt catcttacac ttatgtcact attagaaaaa
     6841 atgacgctta atccagcttt gctttatggt tttgatgcgg gttatttggc agaaaatggt
     6901 ccagcagatt tggttatttt tgcagacaag caagaacgtc tgatcactga gaattttgct
     6961 tctaaagcta gtaattcccc atttattggt aacaaactca aaggtgtcgt gaaatacact
     7021 attgcagacg gagaggttgt ttactctaat taacaaatga gttaaaacaa aaaagcttga
     7081 ctttcagagg aagtcaggct tttagtaaat ttatttttta ggcacttgtt tgcttaaatt
     7141 tagcccccaa ggtattagat tttcagtatg atgtttgata cgactaatgt tatctttgtg
     7201 tcttataata ataatgaacg caagtaatat cacaataaaa gttaaaaagt agtcataatt
     7261 tggtaaaaga aaatgaatgg caggaaatgt taaaacagat aacacaccaa cgatagctga
     7321 aaccacacta gctaaagata tcatgctaaa taaatagaga actaaaacaa agatagatgc
     7381 taaaaaaaag agatataacg gagcaaagcc tagcaataca ccagcacttg ttgctacggc
     7441 cttaccacct ttaaagttgg caaaaatagg aaaagtatgc cctaaaactg cgaaaaagcc
     7501 gatagcaatg gatgaaattg aagtcatacc aaaaataatt ggtaacaata ttgaaagtgt
     7561 ccctttaaac atatcaatag ctaaggtagc tgttcctgcc ttgacaccta aaatccgaaa
     7621 agtatttgtg gttccagtat ttcctgatcc atgctctcgt aagttgatgt ggtaaaagta
     7681 ctgtccaatc catagtccag ttggaataga accaagtaaa taggcaatgg taataaaaag
     7741 tagtaatttc ataatcctta ttataacata ttcatagatt ggagaactag atctggaaaa
     7801 gaattcggaa aaaattagca aattattttg caaaattggt aaaaaacttg taagataaaa
     7861 aagatgaaac tgtctggagg tcggtgttga ctaagaaaga aattacaatt aataattata
     7921 atgacgatgc tatccaagtc ctagaagggt tagatgctgt tcgcaagcgt ccagggatgt
     7981 atatcggttc aacagatgct acgggattgc accacctgat ttgggaaatt gttgataatg
     8041 ctgtcgatga ggccttgtct ggttttggag atgatattaa ggttgttatc aataaagatg
     8101 gctcggtcag tgtggcagat agcggacgtg gaatgccaac tggtcagcat gccatgggga
     8161 ttcccaccgt tcaagttatt tttacgattc ttcatgcagg tggtaaattt ggtcagggtg
     8221 gctataaaac gtcaggtggt ttgcacggtg ttggctcctc tgttgtcaat gctttgtctg
     8281 cctggttaga agtcgaaatc acaagagacg gggcagtgta tcgtcagcgc tttgaaaatg
     8341 gggggaaacc tgttacaacc cttaaaaaag ttggcacagc tcctaagtct aaatcaggaa
     8401 cggttgtaac ctttatgcct gatgataaga ttttttcaac tattgacttt aagttcaata
     8461 ccatttcaga acgattgaaa gaatctgctt ttcttttgaa aaatgtcaag atgagcttga
     8521 cagatcttcg tggagatgac ccgattattg aagagtttca ttatgaaaat ggggttcaag
     8581 atttcgtcga atacctcaat gaagataagg aaaccttgac acctgttatt tacatggaag
     8641 gtcaagacca agactttcaa gtcgaggttg ctttgcagta taacgatggt ttctccgaca
     8701 atatcttatc ctttgtaaac aatgtgcgca ccaaagatgg tggaagccat gaaacgggac
     8761 taaagtcagc tattaccaaa gcgatgaatg actatgctcg taaaaccaat ctcttaaaag
     8821 aaaaggataa aaatctagag ggctcagatt atcgagaagg actctcagca gtattatcta
     8881 tcttagttcc tgagcagcac ttgcaatttg aaggacagac caaagacaaa ttgggaagtc
     8941 ccttagcgag acctattgta gagagcattg tttctgaaaa attaaccttc tttttactcg
     9001 aaaatggaga agtagcttct catttagttc gcaaagccat taaagctaga gatgctcgcg
     9061 aagctgctcg aaaagcgcgt gatgacttac gtaatggtaa gaaaaacaaa aaagacaaag
     9121 gcttgttgtc gggaaaatta accccagctc agtccaaaaa tgccaagaaa aatgaattgt
     9181 atctggtcga aggagattct gcgggaggtt cagccaaaca aggacgagac cgcaaatttc
     9241 aggctatttt accattacgt ggcaaagttt taaataccga aaaagctaaa atggctgata
     9301 tcttgaaaaa tgaagaaatt aacactatgg tttataccat tggagctgga gtgggagctg
     9361 actttaacct cgaggatatc aactatgata agattattat catgaccgat gccgataccg
     9421 atggtgccca cattcaaacc ctcttattga ccttctttta ccgttatatg cgaccattag
     9481 tagaaacagg tcacgtttat attgcgcttc ccccgcttta caaaatgtca aaaggtaaag
     9541 gcaaaactga aaaaattgct tacgcttgga cagatggcga attggaagat ctgcgcagag
     9601 aatttggcaa gggggctatt cttcaacgct ataaaggact gggtgaaatg aatgctaacc
     9661 aattatggga aaccactatg gaccctgaaa ccagaaccct tattcgtgtt actattgacg
     9721 acttggcaag agcagagcga cgtgtttctg tgttgatggg tgacaaagca gccccacgcc
     9781 gtcagtggat cgaggataac gtcaagttta ccctagaaga aaatacggtg ttttagtcgt
     9841 tttgtctctg agaatctcga tgtgaaacta gtaatcatgt ccttgtgaaa agggggcctt
     9901 ttatgaaatt ggtgatttgg caaaacacat acagtcttca gtgggacggg acctatcatt
     9961 ttgctttgga atcttacccg atgatccagg attgggaatt agaaaaaata gctgcttttt
    10021 gtcattatga gaggatgaat caccgggaac ctcagatcat ttgtaaagat caagtgatag
    10081 taacaaaaat taatcagtat ttaaaacata ataatagaaa gccgccattt actccaagcc
    10141 ataagaaggt tgcctcaacc tatgatgttt ctggaaaagc tgtctatggt gattggctta
    10201 gtcatacgtg tacggtggaa acggcaatag cagtctttaa gtcaggaaaa ctcctatcag
    10261 cagttaaggc ttttaaccgt ccggctgagg aattagtgaa agatagtaga aatgctgcag
    10321 gagatcccgc ggactacttt aactatgtga tgttggggtg gtcaaatact acctcaggtt
    10381 atcgtttggc tatggaaagg ctcattggcc atttaccaaa tgatagggaa ttaaatgagt
    10441 tatttatccc tggtgttagt ttccattttt cttatgagga ggtcttagca caagagcatt
    10501 atttatttga tggttatcac cctgccaaag ttaaaaatca cctatcactg gatgctttaa
    10561 aggcgtgtat tattcctcta gatcaagctt ctttgtttga agctattatt ccagaggtac
    10621 ttaaagcacg atgtttttat ttaccttacc atcaggaagg attgatagag tggactgatt
    10681 ctgtttatcg ctttttagta aaccttgaga tggccgatta gttttcccga tggcttacgt
    10741 tatcgaagac atctcttttt cctaaaatct tggttttcga gtgattttca cgttcctctt
    10801 gcttcaactt ttaagaaagg atttgaactc aggttaacat aataaaagac atcagcggtt
    10861 aagttgtttt ttgaacgacc gtagttgtcc ttcttgatag cctgtgcatc atgaacttat
    10921 gtcaaacatt cagaatatgt cccttgagga cattatggga gagcgttttg gacgttactc
    10981 caagtacatt attcaagaac gggcacttcc tgatattcga gatggattga aacccgttca
    11041 gcgccgcatt ctttattcga tgaataagga tggtaatact tttgaaaaag gttaccgtaa
    11101 atcagccaaa tcagttggta atatcatggg gaatttccac ccgcatgggg attcctctat
    11161 ttatgatgcc atggtacgga tgagtcaaga ttggaagaac cgtgaaattc ttgtggaaat
    11221 gcacggaaat aatggttcaa tggatggcga tccgcccgcg gctatgcgtt atacagaagc
    11281 tcgcttgtct gaaattgcgg gttatcttct tcaagacatt gagaaaaata cggtctcttt
    11341 tgcgtggaac tttgatgaca cagaaaaaga acccaccgtt ttaccagcag cttttcctaa
    11401 cttacttgtc aatgggtctt ctggtatttc cgcaggttat gcgacagata ttccaccgca
    11461 caatttgtca gaagtgattg atgccgttgt ttacatgatt gaccatccca aggctagctt
    11521 agagaaatta atggagtttt tgcctgggcc agatttcccg acaggaggca ttatccaagg
    11581 agctgacgaa atcaaaaaag cctacgaaac aggtaaggga cgtgtcgttg ttcgctctag
    11641 gacagaaatc gaggaactta aaggtggtaa acaacaaatt attgtcactg agattcctta
    11701 tgaagttaac aaagcagtct tggtcaaaaa gattgacgat gttcgtgtta acaataaagt
    11761 cccaggcatc gtagaagtac gtgatgaatc tgatcgcaca ggtcttcgca tagctattga
    11821 gttgaaaaaa gatgcagata gccaaaccat tctaaattat ttgttgaaat acacagacct
    11881 tcaagttaat tataatttca atatggttgc cattgatcac ttcacaccac gtcaagtagg
    11941 gcttcaaaaa atactatcaa gctatatcag tcaccgcaaa gatattatta ttgagcggtc
    12001 taaattcgat aaagccaaag ctgaaaaacg cctccacatt gttgaaggat tgattcgtgt
    12061 cttgtccatc ttagatgaga ttattgcttt gattagatcg tctgacaata aggctgatgc
    12121 taaagaaaat ctgaaagtca gctatgattt ctctgaggaa caagcagaag cgattgtaac
    12181 cttgcagctt tatcgtttaa ctaataccga tattgttact ctccaaaatg aagaaaatga
    12241 tttaagagac ttaatcacga ccctttccgc tattatcggt gacgaagcaa ccatgtataa
    12301 cgtcatgaaa cgtgaattaa gagaagtgaa aaagaaattc gctaatcctc gtttatcaga
    12361 gttacaagca gaaagtcaaa tcattgagat tgacactgcc agcttgattg cagaggaaga
    12421 aacttttgtt agtgtgacac ggggcggata cctcaaaaga accagcccac gatccttcaa
    12481 tgcgtctagt ttggaagaag ttggcaagag agatgatgac gaactcatct tcgttaaaca
    12541 ggccaaaaca acagaacacc ttttgttgtt tacaacttta ggcaatgtca tttaccggcc
    12601 aatccatgaa ttgaccgatt tacgttggaa ggatatcggt gaacatttat cacaaaccat
    12661 ttcaaacttc gcgacagaag aagagattct ctacgctgat attgtgactt cctttgacca
    12721 gggcttgtat gtggctgtca cacaaaatgg ttttatcaaa cgctttgatc gtaaagaact
    12781 aagtccttgg cgaacttata aatctaaatc aaccaaatat gtgaagctaa aggatgacaa
    12841 agaccgcgtg gttaccttgt caccagttat tatggaagac ctcctattag tgactaaaaa
    12901 tggttatgct ttgcgattct ccagtcagga agtgcctatt caagggttga aatctgctgg
    12961 tgttaaaggc attaacttga aaaatgatga cagccttgcc tcagcctttg ctgtgacaag
    13021 taactctttc tttgtcttaa ctcagagggg ttctctcaaa cggatggctg tcgatgatat
    13081 tcctcagaca agtcgtgcca atcgtggact attggtccta cgagagttaa aaaccaaacc
    13141 tcatagagtt tttctggccg gaggcgttca atcagatact tcagctgagc agtttgattt
    13201 gttcactgat atacctgaag aagaaactaa tcaacaaatg cttgaagtca ttagtaagac
    13261 tggtcaaacc tacgagattg ctttagaaac gctttcgtta tcggaaagaa ccagcaatgg
    13321 ctcctttatt tcagacacta tttcggatca agaagtactg gtagctagaa cacgttaaaa
    13381 agagtgaaaa ctctttttaa ttttatggta aatttagaat attaatgcaa aagagtagaa
    13441 taaacttgaa aattattgaa aaaaagttac aatagaaaaa agcagaaaag aggtacgatg
    13501 atgacaatag caattgattg ggacaaccta ggatttgaat atcataagtt gccatttcgt
    13561 tacatttctt attacaaaaa tggtcaatgg gataaaggtc aattaacaga agatgccacc
    13621 ttacatatct cagaaagcgc tccagcgctt cattatggac aacaagcttt tgaaggctta
    13681 aaggcttatc gcaccaaaga tggctctatt caactattta gaccagaccg taatgcagtg
    13741 cgtttacaag ccacagcaga ccgcttgttg atgccacaag tatcaacaga gcaatttatt
    13801 gatgcagcta agcaagtggt taaagccaat gaagactttg taccacctta cggaacaggt
    13861 gcaacgcttt acctgagacc gttattaatt ggagttggag atattattgg ggttaaacca
    13921 gccgaagaat atattttcac catttttgcc gtgccagtag gaaactattt caaaggtgga
    13981 cttgctccaa ctaattttat tgtttctgaa gcgtttgacc gcgccgctcc ttacggaaca
    14041 ggtgctgcta aggtgggagg aaactatgca ggaagtttgc taccaggtaa ggccgctaaa
    14101 tcagcaggct tttctgatgt gatttactta gatccagcaa cccatactaa aattgaagaa
    14161 gtcggagctg caaacttttt tggcattact gaaaataatg aatttgttac gccacttagt
    14221 ccatctatct tgccatctat tacgaagtat tcactcttac aactagctga agaaagactt
    14281 ggtatgacag taattgaggg tgatgttcca attaatgaat tggataaatt tgtggaagca
    14341 ggagcttgtg gaacagcagc tgttatctct ccaattggcg gtattcagta caaggataac
    14401 ttacatgtct tttacagtga aacagaagtt ggtcctgtga cacgccgttt atacgatgag
    14461 ttggtcggca ttcaatttgg ggatattgaa gcaccagaag gttggattgt aaaagtagat
    14521 taaaatagag cttgctcttg caagcttttt tgattttgtg taaaatagac aaagtgaggt
    14581 aaagtgatga gtaaaaaaga taaaaaaatt gaaatccaac tgattgatca caaagttatg
    14641 gttaatgaga ctaagattga tggctatcaa ttacaaattg gtaaacgtgt ggttggagaa
    14701 attgcagaac ttgatgagaa atttgctgtc ttaaaaaatg atggagtaga ttgcttcttc
    14761 aaaacgctcg aacaagcggt tgaaaatatc attgaaaact acaatttgaa ccattaaaat
    14821 tagacaaaaa cacttgccag cttacaaaaa ctatgataga at
//
LOCUS       DAUCWU010000034        14848 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUCWU010000034 DAUCWU010000000
VERSION     DAUCWU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.034"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            46..459
                     /locus_tag="VGY24_000974"
     CDS             46..459
                     /locus_tag="VGY24_000974"
                     /inference="COORDINATES: protein motif:HMM:NF013537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283960.1"
                     /translation="MGTAVAYAEEIPTTPILTLEVTERREAINKINRLNDQYPNDPEI
                     QATLEDTTNKILAFESADITEALIAEVNSEVIRLNEEKIARDALDTAEKADAEKRHDF
                     LKQQDAVAIAIQELANIPAEKAFRQYANDNGVDGV"
     gene            580..771
                     /gene="grab"
                     /locus_tag="VGY24_000975"
                     /pseudo
     CDS             580..771
                     /gene="grab"
                     /locus_tag="VGY24_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
     gene            1009..1551
                     /locus_tag="VGY24_000976"
     CDS             1009..1551
                     /locus_tag="VGY24_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4283961.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIDHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1560..2843
                     /locus_tag="VGY24_000977"
     CDS             1560..2843
                     /locus_tag="VGY24_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ4283962.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCEDSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2859..3719
                     /locus_tag="VGY24_000978"
     CDS             2859..3719
                     /locus_tag="VGY24_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ4283963.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3721..4686
                     /locus_tag="VGY24_000979"
     CDS             3721..4686
                     /locus_tag="VGY24_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ4283964.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4797..6080
                     /gene="aroA"
                     /locus_tag="VGY24_000980"
     CDS             4797..6080
                     /gene="aroA"
                     /locus_tag="VGY24_000980"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ4283965.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGIRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSRLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTCGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6073..6564
                     /locus_tag="VGY24_000981"
     CDS             6073..6564
                     /locus_tag="VGY24_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ4283966.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIENQVLKDLLFANDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6772..8223
                     /locus_tag="VGY24_000982"
     CDS             6772..8223
                     /locus_tag="VGY24_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ4283967.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8272..9660
                     /gene="rlmD"
                     /locus_tag="VGY24_000983"
     CDS             8272..9660
                     /gene="rlmD"
                     /locus_tag="VGY24_000983"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ4283968.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKTSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTIMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFIADTAEHAMATWVKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLTKVQPVDLFPQT
                     HHVETIALIQKM"
     gene            9891..10664
                     /locus_tag="VGY24_000984"
     CDS             9891..10664
                     /locus_tag="VGY24_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEQ4283969.1"
                     /translation="MNFDYFYNRQSEMYNFIRLPMVLMEDEIFESISIEAKVLYSYML
                     NRMGLSYKNGWIDEDGKVFIYYTMDAIKEQFNCANDKALKIINELDIKSGIGLIEKKR
                     QGLGKPNRIYVKDFMSVFSDSNNRNPDFRKAEVQTSDNRNSRTPNNRLQDFRKSEGNY
                     NNISNNELRKNDFSKGQKPYGIYKNIFLTDEEYKDLTNELGSRISDYIDRLSSYMKAN
                     NRVYQDHKATIINWYLNDQAKNINDNTTRKMNYDIGESL"
     gene            10661..11488
                     /locus_tag="VGY24_000985"
     CDS             10661..11488
                     /locus_tag="VGY24_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ4283970.1"
                     /translation="MTSLKDFVLRENDIERNGHIYCKVCGKRVDGELLDLGFTKFIPR
                     IKCECEIKRDKENEERERLMKISSLKRDCFSSPLQHQYTFEKYLNEKGQAYKVAYNYA
                     KSFEQMKEDNVGLLFYGDVGSGKTYLACAIANELIEREQVKVKIMNLSQVINQIQKSA
                     FKLDSNEIINNLSNIPLLILDDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSL
                     EIIQNPNIELEYQRIYSRILEMTIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD"
     gene            11481..11966
                     /locus_tag="VGY24_000986"
     CDS             11481..11966
                     /locus_tag="VGY24_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEQ4283971.1"
                     /translation="MINEEVSRSSLNLEVRLAKATSKAILDALKKVHKQIEEQGGLKN
                     VIKNNGEEVKLKDMVKKGQLEEINLKDPELKELKKILNKHGVKFSVMKDKETGNHSVF
                     FQSKDIKVMEHAFKKAVKASERKADRKDSITKTINKFKDMAKDTISKDKVKNKHKEQS
                     L"
     gene            11963..12697
                     /locus_tag="VGY24_000987"
     CDS             11963..12697
                     /locus_tag="VGY24_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ4283972.1"
                     /translation="MISNLKTFENKNFGKLTVIEKDGEFFFIANEVATMLGYVNPRKA
                     VYDHVDEEDKGVTKWNTPGGIQNISIINESGLYSLILSSKLPQAKIFKAWVTREVLPS
                     IRKNGGYIVGQEKKTNEELLADAILVANRIIAEREEEIEELKPKADYYDKLVDYNLLT
                     NFRNTAKELGIPQNQFISFLMDKGLIYRDKKRKLLPYADKNKGYFEVKEWVDPLGTLV
                     GIQTFITPKGRHYLLILLDSEGFYDE"
     gene            12690..14486
                     /locus_tag="VGY24_000988"
     CDS             12690..14486
                     /locus_tag="VGY24_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEQ4283973.1"
                     /translation="MNKILEVILSDIKNLIKIDNPKKFILANIPYLSFFYIGNIFSKH
                     INSYVGGDIIDRIMVGISDIGTLSYIPSLNPRDFLVGISIAGLVKLIVYSKGKNKKKY
                     RQGKEYGSARWGESKDIAPYIDPKFENNVLITNTERLTMNSRPKNPKYARNKNVLVIG
                     GSGSGKTRFYVKPNLMQMHSSYVVTDPKGTLVLECGKMLYENGYDIKILNTINFKKSM
                     KYNPFAYLRSEKDILKLVQTIIANTKGDGEKAGEDFWVKAEKLYYTALIGYIYYEAPE
                     EEKNFKTLLDMIDASEVREDDETYMNPIDRLFEALEKKDPSHFAVKQYKKYKLAAGKT
                     AKSILISCGARLAPFDIRELRELMSEDELDLDKIGDRKTALFVIISDTDDTFNFVVSI
                     MYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASII
                     LQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKETIDLYNTSETRS
                     NQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGVRPFLSDKFDITKHKNYKLLED
                     YDKKNLFDVEEYLTNRDKVKLKSNYKINRLNI"
     gene            14505..14780
                     /locus_tag="VGY24_000989"
     CDS             14505..14780
                     /locus_tag="VGY24_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4283974.1"
                     /translation="MCENRKSYLIILNINGEQFILESDTELTRDKKNYIEAICETMYD
                     ESNEWYEDIYDMSPYDIAELFEKTVKEEVGITVTFKAIDLEVSILEN"
BASE COUNT         5319 a         2249 c         2931 g         4349 t
ORIGIN      
        1 tagtaaaagt taattgttta actttaatcg tctgctgcgt ttgtagtggg gacagcagta
       61 gcatatgcag aagaaatccc tactactcca atactcactt tagaggtgac tgagcgaaga
      121 gaagcgatta ataaaattaa cagattaaat gatcagtatc ctaacgaccc tgagattcag
      181 gcaactttag aagatacgac taataaaatt ttagcatttg aatcagctga tattactgaa
      241 gcacttattg ctgaagtaaa ttctgaagtt attcgattga atgaagaaaa aatagcacgt
      301 gatgcattag atacagctga aaaagcagat gcagaaaaac gtcacgactt cttgaaacaa
      361 caagacgctg ttgctattgc tattcaagaa ttggcaaata ttcctgctga aaaagccttc
      421 agacaatatg ctaatgataa tggtgttgac ggtgtttgat cttacgacga cgctactaag
      481 acgtttacag taacagaaat agttactgaa attcctggag atgctctagt tgaaccagaa
      541 aaactaaaag cagaaattcc ttttgttcca ttaacaccag caagtccaat cgctttagat
      601 attaagaaaa ctaaagatac aaaacctgta gttaaaaaag aagaaagaca aaacgttaat
      661 acccttccta caactggtga agagtctaac ccattcttta cagctgctgc gcttgcaata
      721 atggtaagta caggtgtgtt agttgtaagt tcaaagtgca aagaaaatta gttgctattt
      781 gttctagcaa atgaaacaag ggaatcgaaa gattctcttt ttctgatatt taaaaatgct
      841 acagtttata atagcttgaa agactaaatg ttctcaaact cattagaaca agctttaata
      901 aaactaaccc agttgtatca aaggatagat aaagactaag actgttatag aatatttgca
      961 aaagatttgt tataatggac ccaatacagg tattgtgaaa ggtataatat ggatatttgg
     1021 acaaagcttg cagtgtttgc tttttttgag actccgaaag taatattacg gccttttcgc
     1081 tatgaagatc attgggattt ttacagcatg gttaacgaca ctaaaaacct ttattatgtt
     1141 tttccagaac aaaaaactaa ggcagcaagt gactatcttt tagtacatag ttttataaag
     1201 tttcctttag gtcagtgggc aatagaagat aaagcaaccc accaagtaat aggttctatt
     1261 agaattgatc attatgatgc taaaacgcgt tgtgctgata ttggctattt tttaaactat
     1321 gccttttggg gacaaggaat tatgacagaa gtcgtaataa aacttgttta tttatctttt
     1381 catgaatttg gtctcaaaac gttgcgtatt ataactcatt tagaaaataa ggccagccag
     1441 aaagtagcta aaaaagcagg ttttcaacta aaaacctgtt ttaaaggaag tgatcgtaat
     1501 actcataaaa tctgcattta taaaatgtac caactaacta atgataggtg ataatcatta
     1561 tgagcaaaca tcaagatatt ttagattatt tagaaaaact tgctattggt aaaaaagtga
     1621 gtgttagaag catttcaaac cacttaaaag taagcgatgg aacagcctac cgtgctatta
     1681 aagaggcaga aaatcgaggt attgtcgaaa ccaaaccaag aagtggaact gttcgtattg
     1741 aaaaaaaagg acgggttcgt attgaccgtc tgacctattc cgaaattgct cggatcagtg
     1801 attcggaagt gctagcggga catgctggtt taggacatga attcagtagg ttttcaattg
     1861 gtgctatgac tcaacaaaat attcgtcgtt acttggtcaa aggagggtta ttaatagttg
     1921 gggatcgtga aacgattcaa ctcttagctc ttgaaaacca taatgccatt ttggtgacgg
     1981 gcggatttcc agtatccaaa cgtgttattg aaatggcaaa taaccaacga atcccagtta
     2041 tggtaactca ttatgatacc tttactgtgg ctacaatgat taatcacgcg ctatctaata
     2101 ttcgtattaa aacagatctc aaaacagttg aacaggtaat gatacctatt acagattatg
     2161 gttacctttg cgaagacagc tctgttgaag aattcaatac attaataaaa aaaacacgac
     2221 aagtcaggtt tcctgtatta gattataaaa gaaaagtgat tggtgtagtt agtatgcgtg
     2281 atgtggtgga tcagttgcca acaactaagc ttaccaaggt catgtctaaa aatccaataa
     2341 cggctagacc aaatactagt cttgctaata ttagccagaa aatgatcttt gaagatttaa
     2401 acatgttacc tgtcactgac gaagaaaaca atttacttgg tatgattact cgtagacaag
     2461 caatggaaaa tttgccaaat caccaaccaa ataaccccta tacttatagt gagcaaatac
     2521 tttctaacct tgaagaaacg gttgattatt accaagtagt agttgagcca acaatgattg
     2581 atagtgctgg taacatgtca aacggtgtta tttcagagtt cttaaaggaa attagtatca
     2641 gagccctcac gaaaaagcac caaaaaaata tcattatcga acaaatgatg gtatactttt
     2701 tacatgctat tcaaattgag gatgaattaa aaatatatcc taaaatcatt actgaaaata
     2761 gacgaagcag caccattgat attgaaattt ttgtggatga tcaagtgatt gctaaagcta
     2821 ttattacaac gaaaataaat tagaggagta acaaaacaat gataacatta aaatcagccc
     2881 gtgaaataga agcgatggat agagcaggag acttcttagc aggtatccat attggcttaa
     2941 gggatattat taagcctggt gtggatatgt gggaagtgga agcatatgtt cgtcgccgtt
     3001 gtaaagaaga caacgttctt cctttgcaaa taggggttga tggtcacatg atggactatc
     3061 cttatgcgac ctgttgtggt ctaaatgacg aagtagcaca tgcctttccc cgtcattaca
     3121 ttctgaaaga aggggatttg cttaaagttg acatggtact cagtgagcct ttggataaat
     3181 caattgttga tgtagcagct ttagattttg ataacgtgcc tgagatgaag aaatggacag
     3241 gatcttatac tggtggttta gctgactctt gttgggctta tgctgttgga acaccgtctg
     3301 acgaaataaa gcaattaatg gatgtcacca aagaagccat gtaccgtggt attgaaaaag
     3361 cagtaattgg caatcgtatt ggtgatattg gcgcagctgt tcaagaatac gctgaaagct
     3421 tcggttatgg tgtcgtgcgt gatcttgttg gtcacggtgt tggtcctaca atgcatgaag
     3481 agccaatggt accaaactat ggaacagcag gtcgtggact tcgtctaaaa gaaggcatgg
     3541 tcttgactgt tgagccaatg attaatacag gaacttggga aattgatacc gacatcaaga
     3601 caggttgggc tcataaaacc ctagatggtg gtttatcttg ccaatacgaa caccaatttg
     3661 tgattactaa agatggtccc gttattttaa caagtcaagg agaagaaaga acttactaaa
     3721 atggcagaaa aaaaatggtt tgataaggta ctgtccaagt ggcagtatga acccattcaa
     3781 gtctttatga ggcatttaca aagtgcagaa atggatttat ctgccattgc ggttgcgtat
     3841 taccttatct taacggcctt tccattaatt gttattgcag ccaatatttt tccttacctc
     3901 aatattgata ttgctgattt gttacgttta atgaagcaaa atttgcctaa agatattttt
     3961 agaccggctt ctgcaattgt agaaaatatt ttctctaaac cgtctggaag tgttttggga
     4021 gtagcgactt tgacaggatt atggacaatg tctcgaagtt tgacatcgct gcaaaaagct
     4081 attaataaag cctatggcgc ttctcagcat cgagatttct ttatcggaca cttggttggt
     4141 ttgttgacca gtttaattat tttattttta ttagcctttg ccttaatctt ttcaacattt
     4201 tctaaagctg ctattcaagt gctggataag cattaccatt tgagtgataa tatcacaacg
     4261 attttcttac tcctcattca acctattaca gttttgatta tttttgttgg cttgatgcta
     4321 ctatattttt tattacccaa tgttaagatt aaaaaaattc gctacatttt accaggaacc
     4381 ctatttacat cttttgtgat gacattttta agtaatctgg ttggcaatta cgttgtgtat
     4441 aatgttgagc ggatggtaga tattaaaatg tttggttcag ttatgatttt tatcatcatg
     4501 ctatggttta ttttcttagc aagaatcctg attttaggag ctatatttaa tgcgacctat
     4561 caagaaatgt ccttggggaa attagaaggt cgtagtggtg atatgatcgc tatccttaaa
     4621 aaaacacttg ggaatgattc agatctatct ccttcacagt ctatcgaaga tagtcacact
     4681 gattgataca aaaaagataa taagtcgtgg agtgagaaac tattttcttg cttcattttt
     4741 tatgaggata aatagaaaat cattgatctt agcaaataga aaggcagatg aaacggatga
     4801 aattgcgaac aaatgcagga ccattacagg gaacgattca agttccagga gacaagtcca
     4861 ttagtcaccg ggcagtcata ttaggagccg ttgctaaagg ggaaacacga gtaaaaggac
     4921 tcttaaaagg agaagatgtg ctttccacga ttcaagcctt taggaatttg gggatccgta
     4981 tcgaagaaaa agatgaccag cttgtgatag aaggtcaagg ttttcaagga ttgacagctc
     5041 cttgtcagac acttaatatg ggaaattctg gaacctctat gcgcctcatt gctggattat
     5101 tggcaggtca accttttagt gtcaaaatga ttggcgatga aagcttatcc aaacgtccta
     5161 tggatcggat tgtttatccg ttaaagcaaa tgggggttga gatatctgga gaaacagata
     5221 ggcaatttcc tccactacaa ttgcaaggta atcgtaattt acaaccaatc acatatacct
     5281 tgcctatctc ttctgctcag gttaagtcag ctattttatt agcagctttg caagcaaaag
     5341 gaacaacaca ggttgttgaa aaagagatta ctcgaaacca tacagaagaa atgattcaac
     5401 aatttggtgg acgattgata gttgatggta agcgcattac tttggtggga ccccagcagc
     5461 tcactgctca agagataact gtccccggag atatttctag tgcagctttt tggcttgtcg
     5521 ccggcttaat tattcctggt tcggagctcc ttttaaagaa tgttggagtc aatccgacac
     5581 gaacagggat tcttgaagtg gttgaaaaaa tgggagcaca gattgtctat gaagatatga
     5641 ataagaaaga gcaagtaact agtattcgag tggtatattc tcgcttgaaa ggaactatca
     5701 tatcaggagg tcttatccct cgattgattg atgaacttcc catcattgca cttcttgcaa
     5761 cccaagcaca aggaacaacc tgtattaaag atgctcaaga attacgagtc aaagaaacag
     5821 atcgtattca agtagtaact gatactctta acagtatggg agctaatatc aaagcgacag
     5881 cagatggcat gattatcaaa ggccctacgg tactatatgg ggctaacaca agtacctgtg
     5941 gagatcatcg tattggtatg atgacagcaa ttgcagcctt gcttgttaaa caaggacagg
     6001 ttcatttgga taaagaagaa gccatcatga cgtcttatcc gacgtttttc aaagatttag
     6061 aaaggctgtg tcatgactaa agtgttattg ggatttatgg gagtggggaa aacaacggtt
     6121 tcgaaacacc taagtatgca ctgtaaagat atggatgcta ttattgaagc taaaatcggt
     6181 atgtctattg cagctttttt tgaacagcat ggagaaatag catttcgaac aattgaaaat
     6241 caagtgttaa aagatttatt gtttgcaaat gataatagtg tcatcgtaac aggtggcggt
     6301 gtagtggttt tacaagaaaa tcgacaatta ctgagaaaaa atcaccagca taatatttta
     6361 ttggtagctt cttttgaaac tttgtaccag cgtttaaaac atgataaaaa gtctcaaaga
     6421 cctctatttc ttaagtactc taaggaagca ttttatgaat tttatcaaca acgtatggta
     6481 ttttatgagg gactttcaga tttagtaatc agagttgacc atcgtacgcc tgaagaagtt
     6541 gccaacatca ttgagggtta ttagtaggtc attgtcgtaa tctgatattt atggttttaa
     6601 acaggtatct gtctaaagca cttcttatgg caagttggct ttattaaata ttaggaaata
     6661 atcttgatta ttctataatg attaaaattt tgagaggaca atctggctaa tataggttat
     6721 aatggtagta ttcgttaaaa gtgcaattgt aatactaaga gatgataaga tatgacaaaa
     6781 taccccatgg gtggccttag tcaccatgaa gaattacgtt atttttatct cctaaggaat
     6841 ttgagttacc ttagtgagaa tgaaaaaaaa gagtttgctt ttttaaaatc taaattggaa
     6901 attggtcgag cttatgctcc ttctaagcag cattatagga agtcaaaacg gcaagaacct
     6961 tactttgaag atgactacta taacgactac tcaccaaatg atttactaga agatgatgac
     7021 gtcaatcatg actcttcttt tgttccatat ggtttaccga tatatcctaa agaagacaga
     7081 tatttaaaca aaaaaacaaa acttacagct cgtagaccta tagatactcc gcagcctatt
     7141 gatgaagacg atgctttttt aacagagtct gttgctcgtt gtgctcttcc tagatctcaa
     7201 aaaagaaagc ataagaaaaa aggatgtatg aaatggtttt ttaacattct tggcttattg
     7261 ctaatgactg tgcttatggg gttagggttg atgtttgcaa aaggggtttt tgatatttct
     7321 actaataaag ctaattacaa accagctgtt tcccaagctt ttgatggtca agaaactcaa
     7381 gatggtacga acattctggt tttgggttct gaccaacgag ttactcaagg ttctacagat
     7441 gctcgcacag ataccattat ggttgtcaat gtcggcaacc atgctaaaaa aataaaaatg
     7501 gtctctttta tgcgtgacac tttgattaac attccaggat acagttataa tgataattcg
     7561 tatgatctta aattaaatag tgcatttaat cttggagagc aagaagatca ccacggagca
     7621 gagtacgttc gacgggcctt aaaacataat tttgatatcg acattaagta ttatgtgatg
     7681 gtagattttg aaacatttgc agaagctatt gacaccttgt ttccaaatgg tgttaagatt
     7741 gatgccaaat ttgcgacagt aggtggtgta gctgttgact ccgtagaagt tccagatgac
     7801 ctgcgaatga aaaatggggt tgttcccaac caaaccatcg aagttggaga acaacgcatg
     7861 gatggtagaa ccttgctcaa ctatgctcgt ttccgtaaag atgacgaagg agatttcgga
     7921 cgaaccgtcc gtcagcaaca ggttatgtca gctgttatgt cccaaatcaa agacccaact
     7981 aaactcttta cagggtctgc ggctattggt aaaatctatg cgttgacttc gaccaatgtt
     8041 tcttttccat ttgttgttaa aaatggtgtt tctgttttag gaagtgggaa aaatggagtt
     8101 gagcacgtga ccatcccaga aaacggggat tgggttgacg aatacgatat gtatggagga
     8161 caagcccttt acatagattt tgataagtat cagaaaacgt tagccaaatt aggtctgcgc
     8221 taagatatgg tataatggag agtaactaaa gttgctcttc ttttttgagc aatggttagc
     8281 cctagaaaag gaaaaagaat aagaatgttg aaaaaaaatg atattatcca agttgctatt
     8341 tcagatttaa gccatgaggg agcaggtgtc gctaaacacg atggttttgt tttctttgtt
     8401 gataatgcct tgccagaaga agtgattgac atgcgtgtct taaaggtcaa taaaaccagc
     8461 ggttttggca aagtagaagc ctatcattac ctgtcaccag ctagaaatgc agatgtcaat
     8521 ttaacttatt tgcgaacagg cattgcagac cttggacatc taacttatga agaccaactg
     8581 actttcaaaa agaaacaagt tcaggacagc ctctataaaa ttgctggaat cagtgacgtg
     8641 acagtcgaaa gcacgattgg gatgacagaa cctttagctt accgaaataa ggctcaagtt
     8701 cctgttagac gtgttaacgg tcagctagaa acaggctttt tccgcaagca ttctcatgac
     8761 ttgattccta tttcggatta ttatattcag gacaaagaga ttgatcgtct cattaatttt
     8821 acccgtgatt tattacgtcg ctttgatatt aaaccgtatg acgaaacaga acaaacaggt
     8881 ctcttgagaa atattgttgt tcgccgaggc cattactctg gagaaatgat gctggtctta
     8941 gtgactacac gcccaaaagt tttccgtgtt gaccaagtca ttgaaaaaat cgttgaagct
     9001 tttccagcag tggtgtctat tattcaaaat attaatgata agaacaccaa tgcaatcttt
     9061 ggtaaagatt tcaaaacgct ttacggcaag gataccatta ctgatagcat gcttggaaac
     9121 aactatgcca tttctgctca gtctttctac caagtgaata cgattatggc agaaaaactc
     9181 tatcaaacag ctatagcctt ttcagacctt tctaaagatg acattgtgat tgacgcctac
     9241 tcaggtatcg gaaccattgg cctatcattt gctaaaacag ttaaagctgt ttatggtgtt
     9301 gaagtaattg aagcagctgt tagagatgct caacagaacg ccgctcttaa tggcatcacc
     9361 aatgcctact ttatcgcaga taccgcagaa catgccatgg caacatgggt gaaagatggc
     9421 attaaaccaa gtgttattct ggtagacccg ccacgaaaag gcttaacaga aagtttcatc
     9481 caagccagcg tggcaatggg accacaaaaa atcacctacg tttcctgcaa cccagccacc
     9541 atggcgcgtg acatcaagcg ttatcaagag ttgggttaca aactgaccaa agtacagccc
     9601 gtagatttgt ttccgcagac gcatcatgtg gagactatag cgttgataca aaagatgtaa
     9661 atatagaaat cctgcatttt aagcagtttt ataagcattt tgtcttcgat aaagatgaag
     9721 aaggatacgt caaaaagacc caaaaaaact acatggacgt aagagtagtt ttgcaactgg
     9781 tatgaggaga cacaaagaaa aatataataa gataaaaccc attttatact ttctacaaga
     9841 gtagcttaaa taaggctgct ctttttttat ttcaaagaaa ggaggcacga atgaattttg
     9901 attatttcta taataggcaa tctgagatgt ataacttcat taggttacct atggtattaa
     9961 tggaagatga aatttttgag agcatttcta ttgaagcaaa agttttgtat tcatatatgc
    10021 ttaatcgaat gggtctttca tataaaaatg ggtggataga tgaagatgga aaagtcttta
    10081 tttactacac aatggatgct attaaagagc aatttaactg tgctaatgat aaagccttaa
    10141 agattataaa tgagcttgat ataaaatcag gaattggatt gatagaaaag aaaagacaag
    10201 gacttggaaa accaaatagg atatatgtta aagattttat gagtgttttc tccgattcaa
    10261 ataatagaaa tccagatttc cgaaaagcag aagtccagac ttccgataat cggaattcaa
    10321 gaactcctaa taacagactt caagatttcc gaaaatcgga aggtaactat aacaatatta
    10381 gtaataatga gttaagaaag aatgatttta gtaagggaca aaagccttat ggaatatata
    10441 aaaacatatt tttgactgat gaagaataca aggacttaac aaatgagtta ggaagtagaa
    10501 ttagtgacta cattgatagg ttgtcgtcat atatgaaagc aaataacaga gtgtatcaag
    10561 atcacaaggc aacgataatc aattggtatc ttaatgatca ggcaaaaaac attaatgata
    10621 atacaacaag gaaaatgaat tacgatatag gagagagttt atgacaagtt taaaagattt
    10681 tgtattaaga gaaaatgata ttgaaagaaa tgggcatatc tattgcaagg tatgcggtaa
    10741 aagagtcgat ggagaattac ttgaccttgg atttacaaag tttattccca gaattaaatg
    10801 cgaatgtgaa ataaaaagag ataaggaaaa tgaagaaaga gaaagactga tgaaaatatc
    10861 atcattgaaa agagattgct tctcatcgcc gctccaacac caatatactt ttgagaaata
    10921 cttaaatgaa aaaggtcaag cttacaaggt tgcttacaat tatgctaaaa gttttgaaca
    10981 aatgaaggaa gacaatgttg gacttttatt ttatggagat gttggcagtg gaaaaactta
    11041 ccttgcttgt gcaatagcaa atgaattaat tgaaagagaa caagttaaag ttaagattat
    11101 gaatttatct caggttataa accaaataca aaaatcagca tttaagctag attcaaatga
    11161 aattatcaat aacctctcta atattccgtt gttaatatta gacgaccttg gaattgaaag
    11221 ggatacatct tatgcaagag aacaagtata taacattata aactcaagat acttaaaggg
    11281 taggccgaca atttttacta caaacttatc attggaaatt attcaaaatc ctaatattga
    11341 acttgagtat caaaggatat attcaagaat acttgaaatg acaataccag ttaaagttac
    11401 gggagaagat tttagaagaa aaatccatca agagaagtta agaaaataca aagagttact
    11461 tttatatgga ggtggaatag atgattaatg aagaagtatc taggtcgagt cttaatcttg
    11521 aagtaagact tgctaaagca acaagtaaag caattcttga tgccttaaag aaagtacaca
    11581 agcaaataga agaacaagga ggcttgaaaa atgttataaa aaataacgga gaagaagtaa
    11641 aactaaaaga tatggttaaa aagggacagt tagaagaaat taatctaaaa gatcctgagt
    11701 taaaagaatt aaagaaaatt ttaaataaac acggagtgaa gttttctgtt atgaaagata
    11761 aggaaactgg caaccactct gtgttttttc aatctaagga tataaaggta atggaacatg
    11821 cctttaaaaa agcagttaag gcttctgaaa gaaaggccga tagaaaagat tcaataacga
    11881 agacaataaa taagtttaaa gatatggcta aagacaccat cagtaaagat aaagttaaaa
    11941 ataaacataa ggagcaaagc ttatgataag taatttaaaa acatttgaaa ataagaattt
    12001 tgggaaactc actgttatag aaaaagacgg tgagtttttc tttatagcta atgaagtagc
    12061 aactatgttg ggatatgtca atccaagaaa agctgtttat gaccatgtag atgaagaaga
    12121 taagggtgta acgaaatgga acacccctgg aggaatacag aatatttcaa taattaacga
    12181 atcaggatta tattcactta tcctttcatc aaaactacca caagcaaaaa tattcaaagc
    12241 ttgggtaact agagaagtct taccaagtat tagaaaaaat ggaggatata tagtagggca
    12301 agaaaagaaa actaatgaag agttacttgc agatgcaatt ctggtagcca atagaattat
    12361 tgccgaaaga gaagaagaga ttgaagaatt aaaaccaaag gcagattatt atgacaaatt
    12421 agtagattac aacctactta caaactttag aaatactgcc aaagagttag gaataccaca
    12481 aaatcaattt ataagttttt taatggataa aggattaatt tatagagata agaaaaggaa
    12541 actcttacct tatgcagata agaataaagg atattttgaa gtaaaagaat gggttgatcc
    12601 actaggtaca cttgtaggaa tacaaacatt tataacacca aagggaaggc actacctact
    12661 aattttatta gatagtgaag gtttctacga tgaataagat attagaagtc attctttctg
    12721 atattaaaaa cttaattaaa atagacaacc caaagaaatt tatattagca aacattcctt
    12781 atctgtcctt tttttacatt ggaaatattt tttctaagca catcaattct tatgtaggag
    12841 gagatattat tgatagaata atggtgggaa tttctgatat aggaacttta tcttatatac
    12901 caagccttaa tccaagggac tttttagtag gtatttcaat tgctggtctt gttaagctaa
    12961 ttgtctatag caaaggaaaa aataaaaaga aatataggca aggtaaagag tatggatctg
    13021 caagatgggg agaaagcaaa gatattgctc cttatattga ccctaagttt gaaaataatg
    13081 ttcttataac taataccgaa agacttacaa tgaactcaag acctaaaaat cctaaatatg
    13141 ccagaaataa aaatgtatta gtaataggtg gttctggatc aggtaagaca agattttatg
    13201 taaagccaaa tctaatgcaa atgcactcat cctatgtagt aacagaccct aaagggacac
    13261 ttgttttaga gtgtggaaaa atgctctacg aaaatggata tgacataaag attttaaaca
    13321 caataaactt taaaaagtct atgaaataca atccatttgc ctatttgaga agtgaaaaag
    13381 atattttaaa gcttgttcaa acaattattg ccaacaccaa gggagatgga gaaaaggcag
    13441 gagaagattt ttgggtaaag gctgagaagc tatattacac tgccctcatc ggttatatat
    13501 attatgaagc accagaagaa gaaaagaatt ttaagacgct attggatatg attgacgcaa
    13561 gtgaagttag agaagatgac gaaacctata tgaacccaat tgataggcta tttgaagctc
    13621 ttgaaaagaa agacccaagt cattttgcag ttaagcaata taagaaatat aagcttgcag
    13681 caggaaaaac tgctaagtca attctcatat catgtggagc aagacttgca ccttttgata
    13741 taagagagct tagagaacta atgagcgaag atgaattaga tcttgataaa attggagata
    13801 gaaaaactgc tttattcgta ataatatcag atacagatga tacctttaac tttgtagtct
    13861 caataatgta ttctcaatta tttaatctac tatgtgataa ggcagatgat gtgtatggtg
    13921 gaagacttcc agttcatgtt aggtgtctac ttgatgagtt tgcaaatata ggattaattc
    13981 ctaaatttga aaaactaata gcgacaattc gttctagaga aatatcagca agtataattc
    14041 tgcaagcaca atctcaatta aaagcaattt acaaagacca tgcagataca atagttggta
    14101 actgtgactc tacactcttt ctaggaggaa aagaaaaaac aacagtaaaa gaattatcag
    14161 aaacattagg aaaagaaacc atagacctat ataacacatc agaaacaaga tccaaccaaa
    14221 aatcttttgg tctaaattac caaaaaactg gtaaggaact tatgagtcaa gatgaaataa
    14281 ctgtaatgga tggcggtaag tgtatatacc aactaagagg agtaagacct ttcttgtctg
    14341 ataaattcga tattacaaaa cataagaatt acaagttatt ggaagattat gataagaaaa
    14401 acttgtttga tgtagaagag tatttaacaa atagagataa ggtaaagtta aaatcaaatt
    14461 ataaaataaa taggctaaat atttgagttt atggagaaag caaaatgtgt gaaaatagaa
    14521 aatcatattt aattatatta aatataaatg gtgagcagtt tattctagaa agtgacacag
    14581 aactcacaag ggataagaaa aattatattg aagcaatatg tgagacaatg tacgatgaaa
    14641 gtaatgaatg gtatgaagat atatatgata tgtccccata tgatatagct gagctatttg
    14701 aaaaaacagt aaaagaagaa gtaggaataa ctgttacatt taaagcgata gaccttgaag
    14761 tatccatttt agaaaactag aaataaagtg ttgataggcg atagaaatta aaaaatctat
    14821 cgtctttttt tatactcaaa attaggag
//
LOCUS       DAUCWU010000035        14703 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUCWU010000035 DAUCWU010000000
VERSION     DAUCWU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.035"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(528..914)
                     /gene="rplQ"
                     /locus_tag="VGY24_000990"
     CDS             complement(528..914)
                     /gene="rplQ"
                     /locus_tag="VGY24_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ4283975.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(929..1867)
                     /locus_tag="VGY24_000991"
     CDS             complement(929..1867)
                     /locus_tag="VGY24_000991"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ4283976.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1913..2296)
                     /gene="rpsK"
                     /locus_tag="VGY24_000992"
     CDS             complement(1913..2296)
                     /gene="rpsK"
                     /locus_tag="VGY24_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ4283977.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2314..2679)
                     /gene="rpsM"
                     /locus_tag="VGY24_000993"
     CDS             complement(2314..2679)
                     /gene="rpsM"
                     /locus_tag="VGY24_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ4283978.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2697..2813)
                     /gene="rpmJ"
                     /locus_tag="VGY24_000994"
     CDS             complement(2697..2813)
                     /gene="rpmJ"
                     /locus_tag="VGY24_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ4283979.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2839..3057)
                     /gene="infA"
                     /locus_tag="VGY24_000995"
     CDS             complement(2839..3057)
                     /gene="infA"
                     /locus_tag="VGY24_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ4283980.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3175..3813)
                     /locus_tag="VGY24_000996"
     CDS             complement(3175..3813)
                     /locus_tag="VGY24_000996"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ4283981.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGIAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLHLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3963..5267)
                     /gene="secY"
                     /locus_tag="VGY24_000997"
     CDS             complement(3963..5267)
                     /gene="secY"
                     /locus_tag="VGY24_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ4283982.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5284..5724)
                     /gene="rplO"
                     /locus_tag="VGY24_000998"
     CDS             complement(5284..5724)
                     /gene="rplO"
                     /locus_tag="VGY24_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ4283983.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5938..6120)
                     /gene="rpmD"
                     /locus_tag="VGY24_000999"
     CDS             complement(5938..6120)
                     /gene="rpmD"
                     /locus_tag="VGY24_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ4283984.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6135..6629)
                     /gene="rpsE"
                     /locus_tag="VGY24_001000"
     CDS             complement(6135..6629)
                     /gene="rpsE"
                     /locus_tag="VGY24_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ4283985.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6648..7004)
                     /gene="rplR"
                     /locus_tag="VGY24_001001"
     CDS             complement(6648..7004)
                     /gene="rplR"
                     /locus_tag="VGY24_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ4283986.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7109..7645)
                     /gene="rplF"
                     /locus_tag="VGY24_001002"
     CDS             complement(7109..7645)
                     /gene="rplF"
                     /locus_tag="VGY24_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ4283987.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7848..8246)
                     /gene="rpsH"
                     /locus_tag="VGY24_001003"
     CDS             complement(7848..8246)
                     /gene="rpsH"
                     /locus_tag="VGY24_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ4283988.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8397..8582)
                     /locus_tag="VGY24_001004"
     CDS             complement(8397..8582)
                     /locus_tag="VGY24_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ4283989.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8598..9140)
                     /gene="rplE"
                     /locus_tag="VGY24_001005"
     CDS             complement(8598..9140)
                     /gene="rplE"
                     /locus_tag="VGY24_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ4283990.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9164..9469)
                     /gene="rplX"
                     /locus_tag="VGY24_001006"
     CDS             complement(9164..9469)
                     /gene="rplX"
                     /locus_tag="VGY24_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ4283991.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9548..9916)
                     /gene="rplN"
                     /locus_tag="VGY24_001007"
     CDS             complement(9548..9916)
                     /gene="rplN"
                     /locus_tag="VGY24_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ4283992.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDRTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9941..10201)
                     /gene="rpsQ"
                     /locus_tag="VGY24_001008"
     CDS             complement(9941..10201)
                     /gene="rpsQ"
                     /locus_tag="VGY24_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ4283993.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10227..10433)
                     /locus_tag="VGY24_001009"
     CDS             complement(10227..10433)
                     /locus_tag="VGY24_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ4283994.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10443..10856)
                     /gene="rplP"
                     /locus_tag="VGY24_001010"
     CDS             complement(10443..10856)
                     /gene="rplP"
                     /locus_tag="VGY24_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ4283995.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10860..11513)
                     /gene="rpsC"
                     /locus_tag="VGY24_001011"
     CDS             complement(10860..11513)
                     /gene="rpsC"
                     /locus_tag="VGY24_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ4283996.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11526..11870)
                     /gene="rplV"
                     /locus_tag="VGY24_001012"
     CDS             complement(11526..11870)
                     /gene="rplV"
                     /locus_tag="VGY24_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ4283997.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11886..12164)
                     /gene="rpsS"
                     /locus_tag="VGY24_001013"
     CDS             complement(11886..12164)
                     /gene="rpsS"
                     /locus_tag="VGY24_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ4283998.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12303..13136)
                     /gene="rplB"
                     /locus_tag="VGY24_001014"
     CDS             complement(12303..13136)
                     /gene="rplB"
                     /locus_tag="VGY24_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ4283999.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13154..13450)
                     /locus_tag="VGY24_001015"
     CDS             complement(13154..13450)
                     /locus_tag="VGY24_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ4284000.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13450..14073)
                     /gene="rplD"
                     /locus_tag="VGY24_001016"
     CDS             complement(13450..14073)
                     /gene="rplD"
                     /locus_tag="VGY24_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ4284001.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAVFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14097..14690)
                     /gene="rplC"
                     /locus_tag="VGY24_001017"
     CDS             complement(14097..14690)
                     /gene="rplC"
                     /locus_tag="VGY24_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ4284002.1"
                     /translation="MTQIFTESGEFIPVTVIEATPNVVLQVKTVETDGYKAVQVGFDD
                     KREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEAGDVVDVTGTSK
                     GKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAGRMGGNRVTVQN
                     LEIVQVIPEKNVILVKGNVPGAKKSLITIKSAVKAAK"
BASE COUNT         4107 a         3088 c         2692 g         4816 t
ORIGIN      
        1 aattttgttg gaaacagctt aaagtgacag attttaaaag taaaaaaaat attttcacct
       61 ccaaattaaa agaggatttt aaaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttgc tcagcttaaa agcggggggg tgttcacctc ataatacaac aaaacgcctg
      361 cttataaaaa gtttctatag cttagatacc tttcataaac aagcgttaca tattaacaat
      421 atgatgagtg ttcccgctag gaaaaatcct agcggtagac cagagctaga ctaagaataa
      481 tctctcgatt actccatcat cataacactc aacaaaattg ataaaaatta aactaattca
      541 ataattgcca ttggagcagc atctccacgg cgtggttctg ttttaagaat acgagtgtat
      601 ccaccgttac gttctgcata acgaggtgca atttctgaga aaagtttttg aagagctgtc
      661 gttgatgtgt atttatcggt agcttcatca taattttctg atgcgatttc gttacgaaca
      721 tatgcagctg cttggcgacg agcatgaaga tcaccacgtt taccaagagt aatcattttt
      781 tcaactgttt tacggatttc ttttgcacgt gcttctgtcg ttacaattga ttcgttgatc
      841 aaaagatctg tcgtcaaatc acgaagcatt gctttacgtt gtgagctagt gcgtcctagt
      901 ttacggtaag ccattttgtc ctcctatatt atttatcgtt ttttagtccg agacctaagt
      961 cagcaagttt aaccttaact tcttcaaggc tcttacgtcc aagattacgg actttcatca
     1021 tttcaggctc agatttttct gttaagtcga agacagtgtt aatgcctgca cgttttaaac
     1081 agttatatga gcgtacggat aaatcaagct cctcgatggt gcggtcaagt accttttcat
     1141 cgttcacttt ttcagtttct ttcattactt cggtagcttt tgcaacctct gttaaatcag
     1201 taaacaggtt taagtgttcg atcaaaactc gagcagatag acctaaagca tcttcaggaa
     1261 taattgttcc gtttgtcatg atttcaatcg ttaatttatc aaagccatca ttgctaccta
     1321 cacgggcagg ttcaacttga taattaactt ttttaactgg cgtatagatt gaatctactg
     1381 ccaatgtacc cacaggtgca tcatcttttt tgttgccttc tgctggcaca taaccacgtt
     1441 ttttagcaac agtcattgtt gcacgtagag aatgtccttc agcgattgta aaaagataat
     1501 gatccgggtt aacaagttcg atatcactgt cagtaagaat atcaccagct gttacctctg
     1561 ctggtccttc tacttcaagt tcgataatct tttcgtcttc gacgtatgat ttcacagcaa
     1621 gtcccttaac attaaggata atttgcatga catcttcacg tacacctggg attgtatcaa
     1681 attcgtgtag tactccatca attttaattg atgttactgc tgcacctgga agtgaagaca
     1741 agagtacacg acgaagagaa ttacctagag ttgttccgta gccacgttca agtggttcga
     1801 tgacaaatct accgtaatct ttattttcat caatttttgt tattattggt ttttcaaact
     1861 caatcatttt ttcacccctc gaaacgaaac ttgtgtacta tttgttatat gattatacac
     1921 gacgacgttt tggaggacga gcaccattat gtggcacagg ggtcacatca cgaattgcag
     1981 ttacttcaag acctgcagct gctagagcac gaatagctga ttcacgacct gaaccaggac
     2041 cttttacagt cacttcaaca gtttttagtc cgtgttcttg tgcagatttt gcagcagctt
     2101 ctgcagccat ttgagcagca aatggagtag atttacgaga acctttgaaa ccaagagcac
     2161 cagctgatga ccatgcaaga gcattaccat gcacatctgt aatcataaca atagtgttat
     2221 taaatgtagc gtgaatgtgt gcaacaccag attcgatgtt ctttttcaca cgacgtttac
     2281 gtgttggttt agccaattgt tttacctcct attttatttt ttcttacctg caatcgcaac
     2341 agctttccct ttacgagtgc gagcattgtt tttagtattt tgtccacgga caggaagtcc
     2401 acgacgatga cggattccac ggtatgaacc gatttccatc aaacgtttaa tgttcatgtt
     2461 tacttcacgt cgaaggtcac cttcaacttt gattgcatcc acttcacgac ggatagcatc
     2521 ttcttgatct gatgttaaat ctttaacacg gatatcttca gaaatacctg cagctgctaa
     2581 gattttttta gatgttgcaa gaccaattcc gtaaacataa gtaagtgaaa ttactacgcg
     2641 tttatcattt ggaatatcaa ctccagcaat acgagccatt ttttctcctt tctattttat
     2701 ccttgacgtt gtttgtgttt tggatttgtc ggacaaatta ccataacacg accattacgg
     2761 cgaataactt tacagtattc gcaaattggt ttaaccgatg gtcttacctt catgttttaa
     2821 tccctccaat tatttcgatt atttaaagcg gtatgtgatg cgtccacgtg tcaagtcata
     2881 aggactcatt tctactgtaa cacggtcacc tactaaaata cgaatgtaat ttttgcggat
     2941 ttttcctgat acagttgcta gaatttgatg tccattttcc aattcaacag taaacattgc
     3001 atttggcatc gtttctacta ctttgccttc aatttcaatc acgtcttctt ttgccacgca
     3061 aaagtacccc ctaaatttcg atttgatgtc ctctgagcac agaggtaaca attataagtc
     3121 agactaaact agtatatcat tactaatcta cttatgcaag tagatttgta taatttattt
     3181 gagttctagc aacgcttttt caacatctgc aaaaacgtct gttatttctt gattaccttc
     3241 aatatctgta acaagaccaa gcttacgata gtgttcaaga ataggttctc cttgagccat
     3301 attgacgtcc aaacgacgtt taacagtttc aggcttatca tcttcacgtt gatagtaatc
     3361 ttcttcttta taatctactg gtgggttgaa cactttatgg aacgtttcac cagttttacg
     3421 attgataata cgaccactca aacgctctac aagacatgat ggatccactt taatattaat
     3481 aacaccatct aagtggagtc ctagttcttc aagtgttgca tctaaggcgt gtgcttgttc
     3541 aatagtacgt ggatacccat caagcaaaaa acctttttct gcgatatcat cctcagctaa
     3601 gcgctctttt acaatcccgt ttgtgacttc atcaggaacc aattcacctt tatcaatata
     3661 acttttagct aaacgtccca tttcggtttg attagccatt gcggcgcgga acatatcccc
     3721 tgttgagatg tgagcaatac caaattcttc aacaatctta gctgcttggg tccctttacc
     3781 agctcccggc aaacccatga ttaaaagatt catcttttcc tcctctttta aaataaatac
     3841 atagtaggaa attattttcc ttggtttttg ttttcaaatg gcaacaagaa atcgtttaat
     3901 gataaccaag tcttattgtc atttaaaaac aaaatagaag gttgacaatg tcaacctgta
     3961 ttctattctg ctgtattcat aaatccgaca tattttctct ttagaagata tccctcaagc
     4021 tgtttcatac cttcgatacc agttgaaatc aaaatgagca aactagttcc acctaaagca
     4081 atactagaag aaaggttgag agcttgttgt gctgctattg gcgctaaaga gataaatgct
     4141 aagaagatag ctcctacggt tgctagtttt tttagcaatg cggacataaa ttgttctgtc
     4201 tcacgtccag ggcgaacact tggtatatat gaggaattct tctgaagatt ttctgctgtc
     4261 ttctcaggat taacttgtac aaaggtatag aagaatgaga acaatataat caacaaggcg
     4321 taaactatca ttccgactgg agtttgataa ttaaaaattt cttgcaaacg gtttaaccac
     4381 ggtaaatctc taccattttg aacaaaagga ataatcgtac ttgggatagt tgtaatcgag
     4441 ctagcaaaga taacgggaat aacgccggct ggatttactt ttaatggaag atatgaactt
     4501 gtaggtgcac cttgcattag ctttgtgtat tggattggaa ttttatattc cgcttgttgg
     4561 acatatgttg taaagaaaac aatagcaaga acagctatga ttaaaatccc aacaatgaga
     4621 taagacgagt gtaaatcact tgccttaaca tttacaaaat agtcttcacg aattgttgca
     4681 attgcacttg gaatagacga aataataccc gcaaagataa tcattgatac gccattacca
     4741 aatcctttat ctgtgatttg ttctccaagc caggtaacaa taacgctacc tgttgtcagt
     4801 aatgccccga tcagtaaata tgttttgatg tctggtgtct taacaagtgc aacgttcgac
     4861 aaagtattaa accctgcagt gataccaatg gattgggcaa aagccaaaac aagtgaaata
     4921 tagcgcgtcg cttgatttaa tttacgacgt ccaacctcac cttgtttgcc ccactcaaca
     4981 aacttaggta aaatatccat ctgcaataac tgaacaacga tagaagcagt aatatatggg
     5041 ctaaccccca ttgaaaatac tgagaaattt ctcatcgcat taccactaac taagtttaac
     5101 atatttaaga aagggagttc acttaattgc tctaagctct tggcatttac accaggtacg
     5161 gtaatatgtg tcccaattcg gaatacaaga atgataaata tagtaaagaa aattttattc
     5221 cgtacagtct ttatctttaa cgcgtctttt agtattttta agaacataat gagtatcccc
     5281 tcattagatg acttcgattg aaccaccttt agcaataata gctgcttcag cagattttga
     5341 gaacttagct gcttttacag tcaatttttt agtcaattca ccgttaccaa gcactttaac
     5401 gcctgatttt tcagctcgga caataccagc atcttttaaa atagccggag tcacttctgt
     5461 accatcatca aaaacattca gttgatcaag attcacaagt gcatattctt tagtattaat
     5521 atttgtaaag ccacgttttg gaatacgacg gaacaatggt gtttgtccac cttcaaaacc
     5581 taaacgtaca ccgccaccgc tacgagcttt ttgacctttt tgaccgcgac cagatgtttt
     5641 accattacct gatgatgtcc cacgaccaac acggttacgt actttacgtg agccttcagc
     5701 agcttttaat tcatgaagtt tcattttttc tccttttttt gtaaaatgct agcgcctcta
     5761 tgagggaaaa ggactcccta tagaaactcg cctatacatt aatgatactt aagttatcca
     5821 aaataactta atacagatac gcactaaccg actactgttt gttgccaaaa agcaaactac
     5881 aagagtcggt tttgtgcacg tttttaatta tgaaatcaga gatttcacag ttataactta
     5941 tttaacatct tcaacggtaa ccaagtgaga aatagctgtc accataccac ggatagcagc
     6001 attatcttct ttgactactg aagagtttaa tttaccaagt ccaagagcaa caacagtttt
     6061 acgttgttct ggcttacgtc cgataggaga cttagtcaaa gtaattttaa tttgagccat
     6121 gatgatcccc tttcttatgc taagtcagaa actgagatgc cacgaagtgc ggcaacttct
     6181 tctgcacgtt taagttgttt caatccttca acagttgcac gaacaatgtt gattggagtg
     6241 tttgaaccaa gagattttga agtaatatca gcaacacctg ctaattcgat gacggcacga
     6301 actgcaccac cagcagcaac tccagaacct tctacagctg gtttcaataa tactttagct
     6361 ccaccgaagt tagtgtaaac ttcgtgaggg attgttgtac caaccattgg tacttcgatc
     6421 atgtttttct tagcagcttc aactgcttta cggatagctt ctggaacttc ttgagcttta
     6481 ccagtaccaa atccaacacg accatttcca tcaccaacaa ctacaagagc tgcaaagcga
     6541 agacgacgtc cacctttaac aacttttgta acgcggttaa tcgcaacaac gcgttcttca
     6601 agttcaactg cattatcttt aaatgccatt attttgtgtc ctccctatta gaatttcaat
     6661 ccgttttcac gagctgcatc agccaaggct ttaacacgtc cgtgatagag atatccaccg
     6721 cggtcaaaca ccacttcaga aataccttta gccactgcac gttcagcaac aagtttgccg
     6781 actacaacgg cttgttctgt ttttgttcct ttagaaacgt ctttatcaag agttgatgca
     6841 cttgcgagcg ttacacccgc tacgtcatca ataacttgag cgtagatgcc tgtattagaa
     6901 cggaatacgt tcaaacgtgg gcgatcagca gttccagaga gtttaccgcg gacgcgacgg
     6961 tggcgttttt ggcggatttt gtttttatct ggtttcgaaa tcacaatttt cacctcttaa
     7021 ttttaatcaa tttgtctcat tgacaagttg tcgattgaac ttatcgagca tgttaaggtg
     7081 caatacttcc catacccgac taagaacatt atttacctgt tttaccttct ttaaggcgta
     7141 cgtattcacc aacgtaacgg atccctttgc ctttgtaagg ctctggtgaa cgcaagctac
     7201 ggatgtaagc agctgtttga ccaacaactt ctttgttgat tccttcaact gagattgaag
     7261 ttgggttagc aacagtgaaa gtaattcctt ctggagcttc aacttcgtct tggtgagatt
     7321 tacctactga aaggacaagt ttagtacctt gaagttgagc acggtaaccg acacccttca
     7381 tttcaagatc ttttttgaaa ccttcagaaa cacctacaac catgttattc aagttagcac
     7441 gggttgtacc atggattgtt ttcatttctt ttgagtcgtt aggacgtaca actgtgattt
     7501 cagtcccttc aactttgatt tcaatatttt tgttgaactc acgagtgagt tcgcctttag
     7561 ggcctttaac agtaataaca ttgttgttat ttgttaattc aacacctgca ggcatagtaa
     7621 ttactttatt accaatacgt gacatgtttg ttttctcctg ttaaattgtc aagccgcata
     7681 gcgactagtt ttcacggggg tttgatagaa tgatatactt caatttatat ttcaaaattg
     7741 aacacgagct gtggcctgtg tgaaaaagat aaatcctcct tgagtgtaaa cacgctacgt
     7801 cagatttcct attttcactt tgtccacgac gctattagta tcttaaatta ccaaacgtaa
     7861 gcgatcactt ctccaccaac attcttttgg cgagcttctt tgtcagtcaa aagaccttct
     7921 gatgttgaaa tgattgcaat tccaagtccg ttaagaactt taggcatgtc atcacgtttt
     7981 gcgtaaacgc gaagacctgg ttttgaaata cgttttaagt tagtgataac acgctcacca
     8041 ttttttccgt attttaagaa cacacgaatg atgccttgtt tatcatcttc gataacttca
     8101 acatttttta caaaaccttc acgtttaaga atctcagcaa tccctttttt aatgtttgaa
     8161 gcaggtactt ctaacacttc gtgttttact tgattagcat tacggatacg tgttaaaaag
     8221 tctgcaattg ggtcagtcat aaccattttt tatattctcc tcttactagc agtttgatgc
     8281 tatcacttac tagttaatgt tgtccttaaa ggactttggt atcagcttat gctcacaccg
     8341 ttttaattaa gcacaagact caatctgaag aacaaattaa gttttgtgtc atatttttac
     8401 caagaagctt tgacaacacc tggaatttga cctttgtaag ccaattcacg gaagcaaaca
     8461 cgacaaagtt taaacttgcg gtaaactgaa tgtggacgtc cacatttttc acagcgagta
     8521 taagcttgtg tagagtgttt tgcaggacgt ttgttcttag caatcataga ttttttagcc
     8581 aatttattta ccccctatta ttttgcaaaa ggcattccaa ggcccttaag caattcacgt
     8641 gattcttcgt cagtatttgc agtagtgacg attacaatat caagaccacg tactttatca
     8701 acatcatcaa agctgatttc tgggaagata agttgttctt tcacaccgag agtgtagttt
     8761 ccgcgtccat caaacgattt tgttggaacg ccatggaagt cacgtacacg aggaagtgaa
     8821 acgctaacca atttgtctag gaattcgtac atacgttcgc cacggagggt aacttttgca
     8881 ccgatcgcta caccttcacg aagacggaag ccagcgattg atttcttagc tttagtaata
     8941 agtggttttt gacctgagat aagtgctaat tcagctgcag ctttctctag gttttttgcg
     9001 ttggatacag catcaccaac acccatattg agaacaattt tctcaacttt tggcacagcc
     9061 ataactgatg tgtaattgaa tttctctgtc aacgcaggaa ttacttcgtt agtatatttt
     9121 tcttttaaac gatttgccat tatgcttctc ctttccttcg tgattaatca agcacttcgc
     9181 ctgatttttt gctgtaacgt acttttttac cgtcaacaac tttataacca acacgtccag
     9241 ctacaccatt tttatctaat acttgaacat tagagacatg gataggtgct tctttttcga
     9301 cgatagctcc ttgaggattt tcagtgttag gtttttggtg ttttttaatc attccaacac
     9361 cttcaacaat aactttgtta acttttggaa gtgctttaag aactacagct tcagtgcctt
     9421 tgtccttgcc agcaattacg cgaactttgt cgcctttttt tacaaacatt ctggtttctc
     9481 ctattatttc ttacgccccg atgggcaccc taggaaaacc taggggacta cgtttgttaa
     9541 cctgagatta aagtacttct ggtgcaagtg atacgatttt catgtagcca ccctcacgca
     9601 attcgcgtgc aactgggcca aagatacgag ttccgcgagg agttctatca tcacggatga
     9661 ttacagcagc attgtcgtca aatttgatgt atgaaccgtc tggacggcgt gcaccagttt
     9721 ttgtacgaac gataacagct ttaaccacat cacctttttt aactgctcca ccaggagtag
     9781 cttgttttac agaagcaacg attacgtcac cgatgttagc gaatttacgt cctgaaccac
     9841 caagtacttt gatagtcaag atctcacgag caccgctatt atcagcaact ttcaagcgag
     9901 tttcttgttg aatcatttca attttctcct tttagtttga ttagataata acagcttttt
     9961 ccactacttc cacaagacgg aaacgttttg tagctgaaag tgggcgagtt tccatgatac
    10021 gaacgatatc gccttcttta gcaacgttgt tttcgtcatg tgctttatat tttttagaat
    10081 agttgatacg tttaccatag actgggtggt tacgtttagt ttcaactaca actgtgattg
    10141 ttttgtccat cttgtcagac actacgcgtc cgtaaagagt tttacgttga ttacgttcca
    10201 tttagaattt ctcctttccc aatctattat ttcatttctg attgcacagt tttaacacgt
    10261 gcaatttgtt tcttaacttc gtcaaggcga gcagtctttt caagttgacc tgctgcagct
    10321 tggaaacgaa gatcaaacaa ttcttttttg agttcgtttt ctttcttagc aagttcttct
    10381 tgagacaatc cacgaagctc tttaacaaaa tctttaattt cttgaagttt catgtcttct
    10441 ccttattctg cttcacgttt tacgaatttg cacttaactg gtaatttgtg gctagcaaga
    10501 cgtaatgctt cgcgagcaat ttcttcagaa acaccagcaa tttcaaacat cactttacca
    10561 cgtttaactg gtgctaccca accttcaggt gcccctttac cagaacccat acgtacaccg
    10621 atagcttttg cagtgtatga tttgtgtggg aagattttaa tccaaacttt accaccacgt
    10681 ttcatgtaac gagtcatcgc aatacgggca gcttcgattt gacggtttgt aatccagtgg
    10741 ctagttgtgg cttgaagtcc gtattcaccg aatgaaactt ctttaccacc tttagcctca
    10801 ccgcgcattt ttccacggaa ttcgcgacgg tgtttaacac gtttaggtac taacatttgt
    10861 tatttgcctc ctttagtgtt tttacgagct ggaagaactt ctccacggta aatccaaact
    10921 ttaacgccaa gtttaccata agttgtgtca gcttcttccc aagcatagtc gatatcagcg
    10981 cgaagcgtgt gaagtggaac agttccttct gaataacctt cagcacgagc gatatcagca
    11041 ccgttcaaac gaccagaaac ttgagtttta atccctttag ctcctgcacg cattgtacgt
    11101 tggattgctt gtttttgagc acgacggaaa gcaacacgtt gctcaagttg acgagcaatg
    11161 ttttcaccaa caaggtgagc atctaaatca ggttgtttga tttcgatgat gttgatgtgt
    11221 acttgttttc cagttaattt gttaagttga ccacgaagag cgtcaacgtt tgcgccacct
    11281 ttaccgataa ccatacctgg tttagcagtg tgaagtgaaa caataacttt gtttactgca
    11341 cgttcaattt caatagttga aactgatgcg tcagctaatt ctttattaat gaatttacgg
    11401 attgcaagat cttcatgaag gtaatccgcg tattcttttt cagcatacca tttcgcatcc
    11461 cagtcacgga tgattccgac acgcatacca attggatgta ctttttgacc cacgatttta
    11521 cctccttatt tttctgatac aactacagtt acgtgagttg tacgtttgtt gattggtgaa
    11581 gctgaacctt tcgcacgtgg acggaaacgt ttcattgttg gtccttcgtt agcgaatgtt
    11641 tcagatacta ccaagtttgc tttttccaaa ccaaagttgt tttctgcgtt agcaattgct
    11701 gagttaagag ttttctcaat aacacgagct gctttgtttg gagtgaattt taagattgcg
    11761 attgcgtcag caactttctt accacggata agatcaagta ctaaacgtgt tttacgaggt
    11821 gaaacacgca ctgtacgagc cattgcttta gctgaagtaa tttctgccat tgtgtcctcc
    11881 tcctattaac gacgtgtttt cttgtcgtca gctgcgtgac ctttgtaagt acgcgttggt
    11941 gcaaattcac caagtttgtg acctaccatg tcttcttgga tgtaaacagg tacatgttta
    12001 cgtccgtcat aaactgcgat tgtatatccg atgaaacttg ggaaaatcgt tgaacgacgt
    12061 gaccaagttt tgattacttt tttcttttcg tcgtttgctt gagcttcaac ttttttcatc
    12121 aaatgctcat cgacgaaagg tcctttttta agactacgtc ccattttgta gttttctcct
    12181 ttaaataatg taccacagcg gcttgaattc aagagcaaga ctccaaactc taccgagttg
    12241 gcggaataaa tagcaaagct attgtaacgc tatttcagca agttttaagc aacttactaa
    12301 gattattttt cgttacgacg acgaacgata agtttgtctg atttagcttt cttgttacga
    12361 gttttaagac caagcgctgg tttaccccat ggagttgatg gcgctttacg tccaactggt
    12421 gctttacctt caccaccacc gtgtgggtga tcgttagggt tcattacaga accacgaact
    12481 gttgggcgga tacctttcca acggctacgt cctgccttac caatattaac aagtgattgt
    12541 tgttcgttac caacagtacc gatagttgca cggcatgtac caagaatcat acgaacttcg
    12601 cctgattgaa gacgaacaag aacgtattta ccttcttgac caagtacttg agcggaagct
    12661 ccagctgcac gaacaagttc tccaccctta ccaggtttca actcaatatt gtgaacaact
    12721 gtaccgacag gaatgtttgc taatggaagt gcgttaccaa ctttgatatc tgcatctgga
    12781 ccagaaacaa tacgttgacc tacttcaaga cctttaggtg caatgatgta agctttcaca
    12841 ccgtcagtgt aatgtacaag tgcgatgttt gcagtacggt ttggatcata ttcgattgtt
    12901 ttaacaactg cttcaacgcc atctttgtta cgtttgaagt cgatcaaacg gtaatgacgt
    12961 ttgtgtccac caccttggtg acgaactgtg atgcgaccat tgttgttacg accagctttg
    13021 ctcttaagag aaacaagcaa tgatttctca ggcgtgcttg ttgtgatttc cgcgaaatcc
    13081 aaagaagtca tgttacgacg gccatttgtc gttggtttat aaactttaat acccacgtta
    13141 tttcctcctt agattattca gcttcagctg cgaacaactc gattgcttta gaatcagctg
    13201 taagagtgat gatagctttt ttagtttttg aagtgaaacc tgtgtaacga ccaacgcgtt
    13261 ttgcttttgg tttaacgtta acagtgttta cacttgcaac tttaactccg tcaaaagcag
    13321 cttcaacagc ttgtttgatc aaaagtttgt gtgcacgagt atcaacttcg aaagtgtatt
    13381 tgcctgcttc aagagcaatc attgacttct cagtgataac tggttttttg attacgtcgt
    13441 acaaattcat tatgcaagaa cctcctcaat tgtagagatt gcttctttag taacaagaag
    13501 tttgtctgcg ttcacgatat caagaacact tgcagttgct gcagttgcaa cagttacgtt
    13561 tggaaggtta cgtgcagaaa gtgctgcaaa ttcatttcct tcttcaacaa gaacaagtac
    13621 ttttgtatca atgctaagag ctgaaagcac ttttgcaaat tcagcagttt ttggtgctgc
    13681 aaatgaaagg ccttctacag ctacaaattt atcttcagca acttttgctg agtaaactga
    13741 tttcaacgca aggcgacgaa ctttttgtgg aagtttgtat ccgtatgaac ggggagttgg
    13801 tccaaagaca acaccaccac cacgccattg tggtgagcgg atagaacctt gacgagcgcg
    13861 tccagttcct ttttgacgcc atggtttacg tccaccacct gatactgctg aacggttttt
    13921 aaccgcatga gtaccttggc gaaggctagc gcgttggcta attacaacat caaaaacaac
    13981 tgattcgttt ggttcgatac cgaagacagc gtcgtttaat tcaactgagc taacttcttt
    14041 accagtttgg tcaaatagtt ttacgtttgc cattttaact gttttctcct ttcttattat
    14101 ttagcagctt taactgctga cttgatagtg ataagagatt tcttagcacc tggtacgtta
    14161 cctttaacaa ggataacgtt cttttctggg ataacttgta caatttcaag gttttgaact
    14221 gttacacggt taccacccat acgtcctgct aagcgtttgt ttttgaaaac gcggttaggc
    14281 gcaacaggtc ccatagaacc tgggcgacga tggtaacgag aaccgtgagc cataggacca
    14341 cgtgattgac catggcgttt gataacacct tggaaacctt taccttttga tgtccctgtg
    14401 acgtcaacaa catcaccagc ttcaaattgt tctacagata attctgcacc aacttctaag
    14461 ccttcaatgt ttttgaattc acgaatgaag cgcttaggag ctgtgtttgc ttttgcaaca
    14521 tggcctttgg caggtttgtt actcaagact tcacgtttgt catcaaaacc aacctgaact
    14581 gctttataac cgtctgtttc aacagtttta acttgaagca caacgtttgg agttgcttca
    14641 atgacagtaa cagggatgaa ttcgcctgat tcagtgaaga tttgagtcat tcccactttt
    14701 ttc
//
LOCUS       DAUCWU010000036        14279 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUCWU010000036 DAUCWU010000000
VERSION     DAUCWU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14279
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.036"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            55..1437
                     /gene="glmU"
                     /locus_tag="VGY24_001018"
     CDS             55..1437
                     /gene="glmU"
                     /locus_tag="VGY24_001018"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ4284003.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKAVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            1468..2022
                     /locus_tag="VGY24_001019"
     CDS             1468..2022
                     /locus_tag="VGY24_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4284004.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            2022..2273
                     /locus_tag="VGY24_001020"
     CDS             2022..2273
                     /locus_tag="VGY24_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284005.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDDKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            2292..2987
                     /locus_tag="VGY24_001021"
     CDS             2292..2987
                     /locus_tag="VGY24_001021"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ4284006.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQTLMTFLENLPV"
     gene            3138..3479
                     /locus_tag="VGY24_001022"
     CDS             3138..3479
                     /locus_tag="VGY24_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284007.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNKGYHQHLTD
                     EKSHLQYSKDNAQLQLRNILDGYQDDLGRNYSSYYYYNLRTVMGLSSEQDIEEHYEKL
                     KDELYRMYNHY"
     gene            complement(3579..4226)
                     /locus_tag="VGY24_001023"
     CDS             complement(3579..4226)
                     /locus_tag="VGY24_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ4284008.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLNINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            4372..5304
                     /locus_tag="VGY24_001024"
     CDS             4372..5304
                     /locus_tag="VGY24_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ4284009.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            5368..6093
                     /locus_tag="VGY24_001025"
     CDS             5368..6093
                     /locus_tag="VGY24_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284010.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            6094..6948
                     /locus_tag="VGY24_001026"
     CDS             6094..6948
                     /locus_tag="VGY24_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ4284011.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(7096..7902)
                     /locus_tag="VGY24_001027"
     CDS             complement(7096..7902)
                     /locus_tag="VGY24_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ4284012.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            8120..10525
                     /locus_tag="VGY24_001028"
     CDS             8120..10525
                     /locus_tag="VGY24_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ4284013.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDETSLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSITKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(10594..10947)
                     /locus_tag="VGY24_001029"
     CDS             complement(10594..10947)
                     /locus_tag="VGY24_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ4284014.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            11191..11616
                     /gene="rplK"
                     /locus_tag="VGY24_001030"
     CDS             11191..11616
                     /gene="rplK"
                     /locus_tag="VGY24_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ4284015.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            11722..12411
                     /gene="rplA"
                     /locus_tag="VGY24_001031"
     CDS             11722..12411
                     /gene="rplA"
                     /locus_tag="VGY24_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ4284016.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            12733..13461
                     /locus_tag="VGY24_001032"
     CDS             12733..13461
                     /locus_tag="VGY24_001032"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ4284017.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            13490..14047
                     /gene="frr"
                     /locus_tag="VGY24_001033"
     CDS             13490..14047
                     /gene="frr"
                     /locus_tag="VGY24_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ4284018.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            14156..>14279
                     /locus_tag="VGY24_001034"
     CDS             14156..>14279
                     /locus_tag="VGY24_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ4284019.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQI"
BASE COUNT         4498 a         2450 c         3080 g         4251 t
ORIGIN      
        1 agaaaatatg ctataatgag aaggtattta aattattata gtaaggaaaa acaaatgaca
       61 aattatgcca ttattttagc agcgggcaaa gggactcgca tgacttctga tctcccaaaa
      121 gttcttcata aagtatctgg actaaccatg cttgagcatg tttttcgtag tgtaaaagcg
      181 attagtcctg aaaaagccgt gacggttatt ggtcacaaat cagaaatggt acgtgctgtc
      241 ttagcagatc aatcagcctt tgttcatcaa acagagcagt taggaactgg ccatgccgtg
      301 atgatggcag aaacacaact agaaggacta gaagggcata ccttggtgat tgcaggagat
      361 actcctttga tcactggaga aagcctgaag agtctgattg attttcatgt gaatcataaa
      421 aatgtcgcca ctattttaac agctacggct caagatccat ttggttatgg tcgtattgtt
      481 cgcaacaaag atggcgaagt gatcaagatt gttgagcaaa aagacgccaa tgagtatgag
      541 caacaattaa aagaaatcaa tacaggaacc tatgtgtttg ataacaagcg tctctttgaa
      601 gcgctcaaat gcatcactac caacaatgct caaggagagt attacctgac tgatgttgtg
      661 gctattttta gagcaaacaa agaaaaggta ggagcctata tcctgagaga ctttaatgaa
      721 agtcttggtg ttaatgatcg tgtggcctta gcaacagctg aaacagtgat gcgtcagcgc
      781 attacccaaa aacatatggt caatggggtg actttccaaa atcctgaaac cgtttatatt
      841 gaaagcgatg ttgagattgc tccagacgtc cttatcgaag ggaatgtgac cttaaaagga
      901 cgtacacata tcggctcagg aactgtcttg acgaatggta cttatattgt ggattctgaa
      961 attggtcaag gaagtatcat caccaactca atgatcgaat catctgtttt agctgcagga
     1021 gttacggttg gcccttacgc ccaccttcgt ccaggaacga ccttagatag ggaagtccat
     1081 atcggtaact ttgttgaggt caagggttct cacattggtg aaaaaacaaa ggctgggcat
     1141 ctgacttata ttggaaacgc tcaggttggg tcttcagtca atgttggggc tggaaccatc
     1201 actgtcaact acgacggcca aaacaaatat gaaacagtca ttggagatca cgcttttatt
     1261 gggagcaact cgactctcat tgcacctttg gaagttggag ataatgcttt aacagcagct
     1321 ggctcaacga tttctaagac agtaccagct gatagcattg ttattggtcg tagccgccaa
     1381 gtcacaaaag aaggttatgc caagcgccta gcgcatcacc caagtcgaag caaataattc
     1441 aagatctatt tagtaaggag gccaggcatg aaatttgaag aaaaaacgct taagagacaa
     1501 acagtttttg atggtcatat ttttaaagtt gttgtggatg atgttgaact tcccaacaat
     1561 ctggggcaat ccaagcgaga attaattttt catcgtggtg ccgttgctgt tttggctatt
     1621 actcctgaac gtaagattgt ccttgtcaaa caataccgta aggctattga aagtgtctca
     1681 tacgagatcc cagcgggtaa gctagagatt ggtgaggaag gctcaaagct aaaagctgct
     1741 gcacgtgagc tagaagaaga aacagcctat acggggacat tgacgttttt gtatgaattt
     1801 tatacagcga ttggtttttg caacgaaaaa atcacacttt tcttagcgac agacctaaca
     1861 caagttgcca atcccaaacc tcaagacgac gatgaagtta tcgaggtctt agagttgaca
     1921 tatcaagagt gcatggactt agtcgctcaa ggtaaacttg ctgacgctaa aacgttgatt
     1981 gctttgcaat actatgcctt acattttgga ggtgagcagt gatgggaagg ccattgttga
     2041 ccgatgatat catcgaaaaa gcgagacgaa tggaaacatt tgagccagat gatgctgtag
     2101 actttgacac aaaagtaatg acgctaccag aaaaagatga taaagcacgc atttataaaa
     2161 gccgtcggat tgaaaatgcc aagcgcagcc aattgcaatc taaattaaat gtgattttga
     2221 tagctgttat gctgttaatt gccatcttgg tatatgctat cttttatctt taaaaaacat
     2281 aaggaaatga tatgaaaata ggaattattg ctgctatgga ggaggagttg tccctgctct
     2341 tagcaaatct cttggatgct caagaacatc aggtgctttc aaaaacttac tatacgggtc
     2401 gttttggcaa gcatgagctc atattggtac agtcaggtgt tggaaaagtg atgtctgcta
     2461 tgacagttgc gatcttggtt gagcatttca aagcccaagc tattatcaat acaggatcag
     2521 caggggcagt cgcttcacat ttagcgattg gagatgtggt ggttgcagat cggttagtgt
     2581 atcatgatgt ggatgctact gcatttggtt atgcttatgg tcaaatggct ggtcaaccat
     2641 tatactatga ttgtgatcca cagtttgtgg ctatcttcaa acaagtccta aaacacgaaa
     2701 agacaaatgg ccaagttggt ttgattgcaa caggagacag ttttgttgct ggtcaggaca
     2761 agattgacca gatcaaaaca gcttttagcg acgtgttagc agtagaaatg gaaggcgcag
     2821 ctattgctca agcagcacat actgcaggga aacccttcat cgttgttcgt gcgatgagtg
     2881 ataccgcagc gcatgatgcc aatattactt ttgatcaatt tattatagaa gctggcaagc
     2941 gttcggccca gacattaatg acatttttag aaaacttacc tgtttaactc actttatcgt
     3001 gagacaatta gctggataat ctctatttta ggagtctggt cacctcttgt tttgaaacct
     3061 aaaaataaga attgcaatca cttgaccaaa cgctataatg ggttaaggaa cgatagatta
     3121 ctaaaaggag tctaaaaatg acactaaaaa aacactatta tcttctcagt ctgctagctc
     3181 ttgtaacggt tggtgctgcc tttaacacaa gccagagtgt cagtgcacaa gtttatagca
     3241 acaaagggta tcaccagcat ttgactgatg aaaaatcaca cctgcaatat agtaaagaca
     3301 acgcacaact tcaattgaga aatatccttg acggctacca agatgaccta gggagaaact
     3361 actctagcta ttattactac aacctaagaa ccgttatggg actatcaagt gagcaagaca
     3421 ttgaagaaca ctatgaaaag cttaaagatg agctataccg catgtacaat cattattaag
     3481 tcaaaggctt aaaaatggag actagcccct caaaaaaaag aggccacact catttaaatt
     3541 taaatgagtg tggcctcttt ttgaggagac gcaaggggtt aaagggctgt gacataaagt
     3601 tgcttagcaa tattttcagg gatgacaagt tccttgtcgc cataacaaat cgtataggtt
     3661 tttgcaaagg tatcaatttg agttaaggtt agctcggtat tgatattgag gtgatgtgct
     3721 tctagatact ggattaaatc aaagtggtcg tggattctgc ttaggcgaaa ccgtcccaac
     3781 tccgtgatgg tgttgagagt tgtagtgttc atttcgacaa gaggctggcc atagcgagga
     3841 atagtccctc cgtgtgggca gaaatctgga aaatcaagga tcttgtctaa acggtcgata
     3901 aaggtgtctg agacagtgtg ctctaagact tcagcttctt ggtgcacttc ttgggtatta
     3961 tagcccagct ggtgaatcaa aaatacctca atcaagcggt gtttgcggta gaggttagca
     4021 acaagagcat aacctttgtc ttttagcaaa tagccttttg ctttatcttt gacaatccag
     4081 ccttgtgaaa tcattttttt aatcatctct gaaacggcag gtgccgaaac gtgcatcttt
     4141 tctgcaacca ttttatttgt aatcttgggt tcttgttctc caatttcata aatacatttc
     4201 aagtaatctt ctttattagg cgtcatatcg tttggccttt ctcttattta taatgattat
     4261 tatatcagaa tttttaggaa aaagcttgac aaaaagccta aaagaggata tccttatagc
     4321 ataattaact aaacttaatt aaataaataa tatatcctaa ggagagaaat gatgggaaag
     4381 agaatgagcc tcattttggg tgctttttta tctgtttttc ttttagtagc ttgttcgtca
     4441 actggcacta aaactgctaa gagtgataaa ttaaaagtcg tggcaaccaa ttcaattatt
     4501 gccgacatga caaaagctat tgctggtgat aaaatcgatc tgcacagcat tgtgccaatc
     4561 ggtcaagatc ctcatgagta cgaaccatta ccagaagatg ttgaaaaaac aagtaatgct
     4621 gatgtgattt tctataatgg tatcaatcta gaagatggtg ggcaagcttg gttcaccaaa
     4681 ctagtgaaaa atgctcaaaa aacaaaaaac aaagattact ttgccgtgtc tgatggcatt
     4741 gatgtgattt acttggaagg tgcaagcgaa aaagggaaag aagatccaca tgcttggtta
     4801 aatctcgaaa acggaatcat ttattcaaaa aacattgcca aacaattgat tgcaaaggat
     4861 cctaaaaaca aagaaactta tgaaaagaac ctaaaagctt atgtggctaa attggaaaaa
     4921 ctagacaaag aagccaaatc aaaatttgat gctattgcag aaaataaaaa attgattgtg
     4981 actagtgaag gctgcttcaa gtacttttca aaagcttacg gtgtcccatc tgcttatatc
     5041 tgggaaatta acaccgaaga agaaggaaca ccagatcaaa tttcatcatt gattgaaaaa
     5101 ctaaaagtca tcaagccatc tgcgcttttt gtagagtcaa gtgtcgatag acgccctatg
     5161 gaaactgttt ctaaagatag tggtattcct atttattctg agatctttac agattcaatt
     5221 gctaaaaaag gtaaacctgg cgatagttat tatgctatga tgaaatggaa ccttgacaaa
     5281 atttctgaag gtctagcaaa ataagataac aataaagcac agcgatttta gtgttgtgct
     5341 ttgttttatt tatgtaaagg agacaaaatg attacaacta acaatctttg tgtgacctac
     5401 gatggcaaca gtaatgcttt agaagcgatt aatgtgacaa ttgaaggtcc ttctatcgtt
     5461 ggtattattg gtcctaatgg agctggaaaa tcaaccttta tgaaagctat cttaaatctt
     5521 attgattatc aaggtcacgt aacggttgat ggcaaagatg gtcgtaagct tgggcataca
     5581 gtagcttacg tagaacaacg aagcatgatt gactataatt ttcctattac tgttaaagaa
     5641 tgtgtggcgc tagggactta tagtaaatta ggcctttttc gccgagtagg aaaaaaacaa
     5701 tttgagcagg ttgataaggt tctcaaacaa gttggtttag aagactttgg ccatcgaccg
     5761 ataaaatcct tatctggtgg tcagtttcag cggatgttgg ttgcgcgttg cttgattcaa
     5821 gaatcagact atattttctt agatgaaccg tttgttggca ttgactcggt cagcgaaaaa
     5881 atcattgtag atcttttgaa agagctcaaa atggctggca aaacgatttt aattgtacat
     5941 catgatttga gcaaagtaga gcattatttt gacaaactca tgattttaaa taaacatttg
     6001 gtggcttatg gcaacgtttg tgaggtattt actgttgata ccttgtcaaa agcttatggc
     6061 aatcatttga ttttaggaaa ggagatggtg taaatgtcta tgaaattttt tgagggcttg
     6121 atgtcatacc actttttaca aaatgcactg ataacggctg tagtcattgg tatcgtctca
     6181 ggtgctgtag ggtgttttat tatccttagg tcaatgtctc ttatgggtga tgccatctca
     6241 cacgctgttt taccaggggt tgctttgtca tttattttag gagtcaattt ttttattgga
     6301 gcgattattt ttgggttatt agcttctgtt attattactt atatcaagga aaattctgtc
     6361 attaaaggag atacggctat tggtatcacc tttagctctt ttttggcact gggagtcatc
     6421 ttgatagggg tagccaatag ttcgacggac ttatttcata ttttgtttgg gaatatttta
     6481 gctgtgcaag atagcgataa gtggattact attggtgttt cgatttttgt tttaggggtt
     6541 attagtctct ttttcaaaga actattatta acatcatttg atcctatctt agccaaatca
     6601 atgggtgtga aggtcaatgc gtatcattat ttgttgatgg tcctattaac tttagtggct
     6661 gttacggcga tgcaaagcgt gggtactatc ttgattgttg ccttattgat tacgccagca
     6721 gcgacagctt atttatatgc taatagctta aaagtaatgt tagtgatgtc atctttacta
     6781 ggcgctttag catcggtttt agggctttat ttgggctata cctttaatgt tgccgcaggg
     6841 tcaagtatcg tactgacttc tgcgatgatg tttttgatca gtttctttgt ttcacccaag
     6901 caaggctacc tcaaaagatg gatgcaaaaa aaagaaaaaa caccttaaac ttttcgcttt
     6961 aaacacctta gtggtttgca aatagtggca aataacatca aagggtgttt tttatatgtc
     7021 atcaaaagac cataaaaaac cttatcaaac caaggtattt agcctattat ctgataaggt
     7081 ttttttaagc atgatttagt ttttaaagcg ataatctttg acaatgtcaa ttgaggtaat
     7141 cgtaatgtct tgttctggtt tatcattttg gttgatagaa gtagcggcaa ttttatcgac
     7201 aacatccata ccatcaatga cttgaccaaa tacagtataa ccgccatcta agcttggatt
     7261 gccgccatgc tcataggcag agatgatagg ttttgggtag ttggtacttg ataatccctt
     7321 gctttgattt ttcttgtttt ggttgatata aaattggcta ccgttagtat tagcgccagc
     7381 atttgccatg gcaagagcac cgcgaatatg atataaaaat ggagagattt cgttgacaaa
     7441 gccattgcca gcatcttttt taggatcctt gcctttccaa atcgattcac cacctgtgcc
     7501 atctcctttg gggtcacctg attgaatcat aaagtcgttg atcacacgat ggaaggtaag
     7561 gttatcatag tagccttttt tagcatgggt gaggaagttc tcaacagcta agggagcata
     7621 ttttgggaat aacttaaggg taatgtcccc ttgacttgtc ctcattacga ccttagcttc
     7681 ttctttgccg acttctttgg ttagttgtgg aaattggcta gcatcagctt tgagagcttt
     7741 ttgaatgctt tcttcatagg ctttagaagc tgcttcgctt tcttctttag cagttttttc
     7801 atcaatgtat ttatcgcctt ttatagcgcg gtcaacagat tcacaagcac tcaaactaag
     7861 taaactaatg gctactaaac tgagagataa taattttttc atgtctactc ctaagaatga
     7921 ttttctttat tttatcataa aatgcaacag aaaatagtag taaagagtta aaaacgccta
     7981 tttttgtgtt ttatgatcta accttggtta aaaggctgct acgactgtta ttatagcaca
     8041 agatgctata gaaaaactta aaacaccttg gtttttttag agctatttcc taatagttaa
     8101 aaaatggtat aatagtgtta tggttaaaag aaatcaaaga aaaaaaagtg cgcctaagaa
     8161 gcgtctgaca aaagcagaag tggaaaagca acgtgccatt aagaggatga ttttatctgt
     8221 tttaatggct ttgttgctta tttttgcgat gcttcgcttg ggcgtctttg gcgtgacaac
     8281 ttataatatg attcgttttt tggtgggtag tttagcctat ccgtttatgt ttgcctggtt
     8341 aatttactta ttttgtttta aatggttacg gcaaaaagat ggaatgattg cgggagttgt
     8401 tattgccttt ttgggattgc tagttgagtg gcacgccttc ttatttgcga tgcctaggat
     8461 gcttgatcaa gatatttttc taggcactgc gcggctaatt acaagagatt tactagccct
     8521 gcgcgtgact gaatttgttg gtggaggaat gcttggtgca ttactttata aacccatagc
     8581 ctttttattt tcgaatatcg gctcttactt tattggtttt ttgtttatct tattaggatt
     8641 atttctaatg acgccttggg atatctatga tgtgagtcac tttgtgaaag aggctgttga
     8701 taaactagca gtggcttatc aggaaaataa agaaaagcgg ttcatcaaac gagaagagca
     8761 ccgcttacaa gctgaaaaag aggctttaga gaaacaagca caagaggaag aaaaacgtct
     8821 agcagaattg acggttgatc ctgagactgg agagattgtt gaggatagtc agtctcaagt
     8881 cagctatgat ttggctgagg atatgccaaa agagccagag attcttgctt atgatagtca
     8941 cctcaaagac gatgagacaa gcttatttga tcaagaagac ttggcttatg ctcatgagga
     9001 aattggggct tatgactctt tatctgcttt agcatcttct gaagatgaga tggatatgga
     9061 tgaacctgtt gaagttgatt tcacaccaaa aacccatctt ctttataaac ttccaacgat
     9121 agatttattt gcaccagata agcccaaaaa ccagtccaaa gagaaaaact tagtccgcaa
     9181 aaatatcaag gtgcttgaag ataccttcca aagttttggg attgatgtca aggtagaacg
     9241 agctgaaatt ggaccatcaa ttactaaata tgaaattaaa ccagctgtcg gggtccgagt
     9301 taatcgtatt tcaaatcttg ctgatgattt ggcacttgct ctggctgcta aagatgttcg
     9361 tattgaagca ccaattcctg gaaaatcctt aataggtatt gaagtgccta actcagaaat
     9421 tgcaacggtt tctttccgcg aactttggga acaatctgat gccaatcctg aaaacctttt
     9481 agaagtacca ttaggtaaag ctgttaatgg taacgctcgc agttttaact tagctagaat
     9541 gccgcatctt ttggtagctg gttcaactgg ttcaggtaaa tctgtggcag ttaatggcat
     9601 tatttcaagt attttgatga aggcacgtcc agatcaagtt aagtttatga tgattgaccc
     9661 caaaatggtt gaattatctg tttataatga tattccacat ttattaatcc cagttgtaac
     9721 caatccgcgt aaagccagta aggcactcca aaaagtggtt gatgagatgg aaaaccgcta
     9781 cgagttattt agtaaaattg gtgtgcgtaa tatagcaggt tataatacaa aggttgaaga
     9841 gtttaatgct tcttctgagc aaaaacaaat accgttgcct ttaatcgttg tcattgtaga
     9901 tgaattggct gacttgatga tggtggctag taaagaagtt gaagatgcta ttattcgttt
     9961 gggacaaaaa gcacgtgctg caggtatcca tatgattctt gcaactcaac gtccctctgt
    10021 agatgttatt tctggtttga ttaaagcaaa tgttccgtcg cgtatggcat ttgctgtttc
    10081 aagtggtact gatagccgta caatccttga tgaaaatggt gctgaaaagc tcttgggacg
    10141 gggtgatatg ctctttaagc cgattgatga aaatcaccct gttcgcttgc aagggtcttt
    10201 catctcagat gatgatgtgg agcgtattgt caactttatc aaagaccaag ctgaagcgga
    10261 ttatgatgat gcctttgatc ctggtgaagt tagcgacaat gatcctggtt tttctggaaa
    10321 tggcggagct gctgaaggtg atcctctttt tgaagaagca aaagccttgg tcttagaaac
    10381 acaaaaagct agtgcctcaa tgattcaaag gcgtttgtcg gttggattta atcgtgccac
    10441 gcgcttgatg gatgaattgg aagaagcagg tgttatcggt ccagcagaag gaactaaacc
    10501 tcgtaaagta ttgcaaacaa actaaaagaa cctgacgtct catcatgaga tgtcaggttc
    10561 tcttttgtga tgtcaagcca gttgatgaga tgcctatgct aatgtaaaaa taaagataac
    10621 tagaatcagg tagaaaataa cggtgactaa aaagccaact cgccaaaata gcttcataaa
    10681 gcgatggtaa gaaaacgact gagttcggat gactagcagt agtgaaatga cgatggctag
    10741 cagcgacatg ataataaggt aataaggcag taaattatgg gtgaaaaatt ttcctgagac
    10801 aagcacaatt tctatcgcaa aaagtaaaag tgcgacatct ggaaatttaa atcccttttt
    10861 attaagttta aataaagagg ttacaatcat ggcaaaaaga ggcgttacta tcaagaaagc
    10921 taaggcagtt aatttataga tcatcatagg tctattttat actcaaatcc cttacttgta
    10981 aactgatact tacctgttgt gatagaaaat gatctttgag agggaaagcg cctacttcaa
    11041 tttaaacatt gcaaaaggca tcagttattt taacaaaaag acttgattta ttttggcaat
    11101 aagctataat agtaaggtat gcaatattgc atatctgtgg gaggtaaaaa tctcaattac
    11161 cgccaaaacc acaacaggag gatttttaaa atggctaaaa aagtcgaaaa acttgtaaaa
    11221 cttcaaatcc ctgctggtaa agctacacca gctccaccag ttggaccagc tcttggtcaa
    11281 gcaggtatca acatcatggg cttcactaaa gaatttaacg ctcgtacagc tgatcaagct
    11341 ggtatgatca tcccagttgt tatctcagtt tatgaagaca aatcatttga tttcatcact
    11401 aaaacaccac cagctgctgt tcttttgaaa aaagctgcag gtgttgaaaa aggatcaggt
    11461 acacctaaca ctactaaggt tgcgacagtt actcgtgcac aagtacaaga aattgctgaa
    11521 actaagatgc cagatttgaa cgctgcaaac attgaagctg caatgcgtat gatcgaaggt
    11581 actgctcgtt ctatgggatt cactgttact gactagtcag tcaagaaccc ctattacccg
    11641 cacgacttca tcataagaga tgagtgacgt gggagatttt aaatcaaatc gatatgacca
    11701 cattacaagg agaattttaa aatggctaaa aaaagcaaac aaatgcgtgc tgcacttgaa
    11761 aaagtagata gcacaaaagc gtacagtgta gaagaagctg tagcattagt aaaagaaacg
    11821 aacttcgcaa aatttgatgc gtctgttgaa gttgcttaca acttgaacat cgacgttcgt
    11881 aaagcagacc aacaaatccg tggcgcaatg gtattgccaa acggaactgg taaaacacaa
    11941 cgtgttcttg tttttgcacg tggtgcaaaa gctgaagaag caaaagcagc tggtgcagac
    12001 ttcgttggtg aagacgacct tgttgcaaaa atcaatggtg gatggcttga ctttgacgtt
    12061 gttatcgcaa cgccagatat gatggctatc gtaggtcgcc ttggacgtgt ccttggacct
    12121 cgtaacttga tgccaaaccc taagactggt acagtaacaa tggatgttgc aaaagcagtt
    12181 gaagagtcta aaggtggtaa aatcacttac cgtgctgaca aagcaggtaa tgttcaagct
    12241 cttattggta aagtttcatt tgatgctgac aaattggttg aaaacttcaa agccttccac
    12301 gatgtaatgg ctaaagctaa acctgcaaca gctaaaggaa cttacatggc aaacgtctca
    12361 atcacatcaa cacaaggtgt tggtatcaag gttgatccta actcacttta agaaaaaaat
    12421 tagggcatcg cctaatcata gcaaaaagac aaatttctga agacaaatct tcactagagg
    12481 tttgtctttt ttatggttta aaatggaatg caaacaaaaa agtgtaggcg ataatatcat
    12541 gaaaggagtt gattctctta atgagatgtg gtgttaacac atcatgagct cacaaaaaat
    12601 gataaaggaa gtatcggtgg tgttaaaaaa gggattttaa ttgacattta ttagcctcta
    12661 aaatgtcctt tctctattca taattgaggg tttgtggtaa aatagtaagg acaaactaag
    12721 gagaacatag tagtggaacc taaatatcaa cgtatattaa ttaagttatc tggtgaagca
    12781 ttagcaggtg aaaaaggagt tggcattgac attccaactg ttcaggctat tgcaaaagaa
    12841 attgccgaag ttcatgtgtc gggagtacag atcgctcttg ttattggtgg aggcaatctc
    12901 tggcgtggag agcctgcagc agacgctgga atggaccgtg ttcaggctga ttacactggc
    12961 atgcttggaa cggtgatgaa tgccctagtc atggctgata gtcttcaaca ttatggtgtt
    13021 gatacccgtg tgcaaacagc tattcctatg caaaatgtag cagaacctta tattcgcgga
    13081 cgtgccttgc gccatctaga aaaaaatcgg attgttgttt ttggagctgg tattggctcg
    13141 ccttattttt caacagacac caccgcggca ctgcgtgctg ctgagattga agcagatgct
    13201 attttaatgg caaaaaatgg tgtggatggg gtctataatg ctgatcctaa aaaagatgct
    13261 aatgcggtta aatttgatga attaacacat ggtgaagtga tcaaacgtgg tctcaaaatc
    13321 atggatgcaa cggcatcgac cttatcgatg gataacgata ttgatttggt ggttttcaat
    13381 atgaatgaag ctggcaatat tcaacgtgtt gtctttggag aacatattgg aactactgta
    13441 tcaaataaag tttgtgacta aaagttagaa aagaaagaga gtaattatca tggcaaatgc
    13501 aattattgaa actgcaaaag aacgttttgc acaatcccat cagtctttat cacgtgaata
    13561 tgccagcatt cgtgcgggtc gtgccaacgc tagtctttta gaccgtatcc aagttgatta
    13621 ttacggggca ccgacgccat taaatcaatt ggcttcaatc actgtgccag aagcgcgtgt
    13681 cttattgatc tcgccttttg ataaatcttc tatcaaagat atcgagcgcg ctcttaacgc
    13741 atcagattta ggcattacac ctgctaacga tggctctgtc attcgtttgg ttattccagc
    13801 tttgaccgaa gaaacacgta aagaattagc caaagaagtg aaaaaagttg gtgaaaatgc
    13861 taaaattgca atccgtaata tccgccgtga tgctatggat gatgctaaaa agcaagaaaa
    13921 agcaaaagaa atcactgaag atgagttaaa aacacttgag aaagatatcc aaaaagcaac
    13981 cgatgatgct atcaaagaaa ttgaccgcat gacggctgaa aaagaaaaag aattgctctc
    14041 agtgtaacag gatttctacg aaaaatagac gactatagac acaaaaagat aggtgaccat
    14101 ttcagaagcg tttggttttg atttgtccct atttttttaa agaaagaaaa aagatatgaa
    14161 tgatttacta gcaacagtca ttactgggct tattaaagaa gaaaacgcta atgactattt
    14221 tatccaaaaa gaagggttta cctttacctt gtctaaagca gaaggagaac gccagattg
//
LOCUS       DAUCWU010000037        14097 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUCWU010000037 DAUCWU010000000
VERSION     DAUCWU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.037"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..310
                     /locus_tag="VGY24_001035"
     CDS             <1..310
                     /locus_tag="VGY24_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ4284020.1"
                     /translation="RSMRLWDDKTFIYNENTDGQTRINIEDNGKIEIAFVDRERLLGY
                     RFVGTAEIQTEGAYYEAAKKWAQGRMGVPKAVGIIHVERIFNLQSGANAGKEINSESN
                     "
     gene            complement(476..1351)
                     /locus_tag="VGY24_001036"
     CDS             complement(476..1351)
                     /locus_tag="VGY24_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ4284021.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYYALHQELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSITVEIDTSLN
                     EHQHHCSSLSNIEVN"
     gene            1487..2008
                     /locus_tag="VGY24_001037"
     CDS             1487..2008
                     /locus_tag="VGY24_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4284022.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLEFIK"
     gene            2223..2741
                     /gene="rimM"
                     /locus_tag="VGY24_001038"
     CDS             2223..2741
                     /gene="rimM"
                     /locus_tag="VGY24_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ4284023.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDVLIGHVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            2731..3462
                     /gene="trmD"
                     /locus_tag="VGY24_001039"
     CDS             2731..3462
                     /gene="trmD"
                     /locus_tag="VGY24_001039"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ4284024.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLL
                     EHYNFSEEERKLLDKIKEALDQGED"
     gene            3462..4454
                     /locus_tag="VGY24_001040"
     CDS             3462..4454
                     /locus_tag="VGY24_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4284025.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGAELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            4632..5690
                     /locus_tag="VGY24_001041"
     CDS             4632..5690
                     /locus_tag="VGY24_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ4284026.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNRFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            5703..6626
                     /locus_tag="VGY24_001042"
     CDS             5703..6626
                     /locus_tag="VGY24_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ4284027.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDLKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
                     ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            6882..7595
                     /locus_tag="VGY24_001043"
     CDS             6882..7595
                     /locus_tag="VGY24_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ4284028.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            7592..8503
                     /gene="pfkB"
                     /locus_tag="VGY24_001044"
     CDS             7592..8503
                     /gene="pfkB"
                     /locus_tag="VGY24_001044"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ4284029.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLNAEGVKTDFVKGDQDTRINVKIKSQEETEIN
                     GQGPVISREQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALAYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            8500..10446
                     /locus_tag="VGY24_001045"
     CDS             8500..10446
                     /locus_tag="VGY24_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4284030.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSEA
                     AVSTVKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            10539..11138
                     /locus_tag="VGY24_001046"
     CDS             10539..11138
                     /locus_tag="VGY24_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4284031.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDSLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            11291..11998
                     /locus_tag="VGY24_001047"
     CDS             11291..11998
                     /locus_tag="VGY24_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ4284032.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(12055..12366)
                     /locus_tag="VGY24_001048"
                     /pseudo
     CDS             complement(12055..12366)
                     /locus_tag="VGY24_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12570..12914)
                     /locus_tag="VGY24_001049"
     CDS             complement(12570..12914)
                     /locus_tag="VGY24_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284033.1"
                     /translation="MLRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEFDFKIRKF
                     LMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVFKKYKDNKTINMK
                     DFSHFDIYLWTK"
     gene            complement(12955..13980)
                     /locus_tag="VGY24_001050"
                     /pseudo
     CDS             complement(12955..13980)
                     /locus_tag="VGY24_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases.; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
BASE COUNT         4398 a         2315 c         2873 g         4511 t
ORIGIN      
        1 acgttctatg cgtctctggg atgataaaac atttatttat aatgaaaata cagatggtca
       61 aactcgtatt aacattgaag ataatgggaa aattgaaatt gcctttgttg atcgtgagcg
      121 ccttttaggt tacaggtttg taggaactgc tgaaattcaa acagaaggtg cttattacga
      181 agcagcgaaa aaatgggcac aaggacgcat gggggtacca aaagcagttg gtattattca
      241 tgtcgagcgt atttttaatt tacaatctgg tgctaatgca ggtaaagaaa taaacagtga
      301 aagtaactaa aattaactgt aacagtattt aggaccctta ttttaaacgc taaaaaacaa
      361 gcatacccct ttttacagca tagtgactat gttgtaaaaa ggggtatgct tattaggtgc
      421 tcttaatatt tctagataac tgcacctaat aagtttttaa tagacttata gcacattagt
      481 tgacttcaat attagaaaga gaagaacagt gatgttggtg ttcgtttaaa gaagtatcga
      541 tttccacagt gattgagttt atattgtacc tttgacaaat cagtctaata gattttttgc
      601 aatgtttttc agtggtagat tctctcaagc agcaatgtat agttgctcta tgatcaatac
      661 catccatcga ccaaacatta agctgattaa cagacactat atgaggaagt tggctgagtt
      721 cctggtgcaa agcgtaatag tctatcgaat caggaacacc atctaaaaaa atgttagcgg
      781 ttgctactaa ctttggaaga gctttagata atataaaaga agcaatggca attgataata
      841 aaggatcaag aatgtaccaa ggtttccatt ttaagatcag tgacagtatg ataatggcta
      901 accacccaag gatatcttct aaaaagtgaa ggcttaatat ttcttcattt tttgtctggt
      961 tagagtgaat gatgaagcta gcaaatccat tgattataat tgcaaaaata gctaaaacga
     1021 acataccgtc ataattaaca atagtaggat gccataattt tggtatcgtt tcaatcatta
     1081 ctaataatga cccgctaata agtattagat ttgtcgttag cgctcctaaa aggctaaatc
     1141 gcttatagcc tagtgaaaaa tttggactct cttttttaac agcctttctt tctaagatag
     1201 ctgagatacc aatagcaatg gcatctccaa aatcgtggac agcatctgct aaaatagcac
     1261 ccgaaaagaa taatgtccca aaaataaatt caatgaggga aaaactaaga tttaatataa
     1321 aaataatggt gactttttta cttgctggca taattttctc cttttgttat actggtaata
     1381 tgtatagtag aattctttat tctattatct aaaagttaaa atacaattac tataaccaaa
     1441 aatgagcacc tgttcaatag tgaacaacat ttagaaagtg tccgatatga caaaagacag
     1501 acaaatcaaa aaaacaaaaa cagctatcta tagtgctttt attgctttat tacaaaaaaa
     1561 agaatattcg aaaataactg ttcgagatat gattacactt gctaatgtag ggagatcaac
     1621 cttttatgca cattatgaaa gcaaagaaat gttactaaaa gaactctgtg aagaactttt
     1681 tcatcatttg tttaggcaaa aaagaaatgt gacttttgaa gactatttgg tacatatttt
     1741 aaagcatttt gaacaaaata aagacagcat tgcgacgttg ttactttcta atgatcccta
     1801 ttttttatta agatttaaaa acgaactaga acatgatgtt taccctaact tgcgctgcaa
     1861 gtatatcgac aaaacgacta ttccagaagt attcctaaaa caatttgttc tatctagttt
     1921 tatagaaacc ttaaaatggt ggctccatca aaggcaaagg atgtctgcaa atgaactatt
     1981 aaagtattat ttagagttca taaaataatg actagtgagt catcttgcca aattcaattt
     2041 tttaatacag acggatttta acaatttagc agagaaagac aaaactgtaa gtgcaaaata
     2101 gactataacg tgataaaata ataggtaatt tggttctgaa aaagaagtgt ctatcaaact
     2161 ttagaatcaa gagaggtcgc taaagtgacg tgagataaag agttaacgat ttaggagcta
     2221 ccatggaata ttttaatgtt ggaaaaattg tcaacacgca aggcctgcaa ggagagatgc
     2281 gcgtgttgtc tgtcagtgat tttgctgagg aacgctttaa aaaaggctca caattagctt
     2341 tatttgatga taaagatcgg tttgttcaag aagtgacaat tgttagtcac cgtaaacaaa
     2401 agcattttga tatcatcaaa tttaaagaca tgtatcatat taatgctatt gaaaagtaca
     2461 agggatacac tttaaaggtc tcaaaagaca atcaagggga tttacaagaa ggagaatttt
     2521 attaccatca gattattggc atggctgttt atgaaaagga tgtccttatt ggacatgtta
     2581 aggaaatctt gcagccaggt gctaatgatg tttgggtagt gaaacgccaa ggaaaacgag
     2641 acttgctctt accatatatt cccccagtag ttcttaacgt agatgttcct aacaagcgcg
     2701 tggatgttga attgatggaa ggcttagacg atgaagattg atattttaac cctttttcct
     2761 gaaatgtttg cccctctaga gcattccatt gtaggcaagg caaaagaaaa aggtctgtta
     2821 gacatccact accataattt tcgagactac gctgaaaaag ctcgtcatgt ggatgacgag
     2881 ccctatggtg ggggccaagg catgttattg agagcgcagc ccatttttga caccattgaa
     2941 cagattgaag ccaaaaaacc aaggattatt cttttagatc cagcaggtaa accctttact
     3001 caggcttatg ctgaagaatt agctcttgag gaagagttaa tttttatttg tggtcattat
     3061 gaaggttatg atgagcggat taagaccttg gtgacggatg aaatttcatt aggcgacttt
     3121 gtattaacag gaggtgagtt ggcggccatg actatggtgg atgcgaccgt caggttaatt
     3181 ccacaggtac ttgggaaaga aagtagccat caggacgatt ctttttcatc aggactttta
     3241 gaataccctc aatacacaag accttatgat tatcgtggta tgacagttcc agatgttcta
     3301 atgagtggtc atcacgaacg tattcgcttg tggcgtctag aagaaagtct caaaaaaact
     3361 tatttaaggc ggccagattt acttgaacat tacaattttt ccgaggaaga gcgcaagcta
     3421 ttggataaga ttaaagaagc attggatcaa ggagaagatt agtgaaagat aaagcatatg
     3481 atattacgat tattggtggt ggtccaatag gtttatttgc agccttttat gcaggtctta
     3541 ggggcgtaac agtaaagatt attgagagtt tgtcagaact tggtggacag cctgccattc
     3601 tttatcctga gaaaatgatt tatgatattc cagcttatcc atctttaaca ggtgctgagt
     3661 tgacagaaaa tttaataaaa caattgagcc gttttgaaga tcgtacaacc atttgtttaa
     3721 aagaagaagt attaaccttt gacaaggtta aaggtggttt ttcgattagg actaataagg
     3781 cagagcattt ttcaaaagcc attatcattg cttgtggaaa tggcgctttt gcaccaagaa
     3841 ctttagggtt agaaagtgag gaaaactttg ctgatcataa ccttttttat aatgttcacc
     3901 agttggacca gtttgcggga caaaaagttg tgatttgtgg tggtggagat tcagcagttg
     3961 attgggcttt ggctttagaa gatattgctg agagtgtgac ggttgtgcac cgtcgtgatg
     4021 ctttcagagc acatgaacac agtgtggaat tattgaaagc atcaacggtc aacctcttaa
     4081 caccttatgt gccaaaagca ttgaaaggaa ttggaaacct ggctgaaaaa ttagttattc
     4141 aaaaagtgaa agaagatgaa gtgttagaac ttgaattgga cagtcttatt gttagttttg
     4201 gtttttcaac ttctaataag aatttgaaaa attggaatct cgactataag cgttcaagta
     4261 ttactgtttc accacttttt caaacaagtc aagaaggtat ttttgccatt ggtgatgcag
     4321 ccgcttataa tgggaaagtg gatttgattg ctactggttt tggcgaagca ccaacagcag
     4381 ttaatcaggc cattaactat atttatccag atcgagataa ccgtgtggtg cattcaacct
     4441 ctttaattga ctaagggagt cggaagtttc aagagcagta gcaacagcta atattcagtt
     4501 tgtcaggaga ggacaaatgg tttttaccta agtcttcgtg acaagtttta tactttgtga
     4561 taaaatagtc attgtgaaaa aagtgtgatg gttttcacag attaacacat tacaatgaaa
     4621 aggggtctat catgacaaca acagataaag agacatttag ttcttttatg aataaggtct
     4681 tggctggtac agcgattgct atcgttgtgg cactcattcc aaatgctatt ttagcgactt
     4741 tcttaaaacc gcttttgcca aacatggcag ctgctgaatt tttacacatt gtgcaagtct
     4801 tccaattctt cacgccgatt atggctggtt tcttgattgg gcaacaattt aagtttaatc
     4861 ctatgcaaca gttggctgtt ggtggagctg cttatatcgg ttctggggct tgggcttata
     4921 cagaagtcat tcaaaaaggt gtggcgacag gaacattcca acttagaggt attggggact
     4981 taatcaatat gatgattaca gctagccttg cggttttagc tgtgaaatat tttgggaata
     5041 gatttggctc tttaaccatt atcttgttac ctatcactat aggaactggc gtaggttata
     5101 ttggttggaa atttttgcct tatgtgtctt acgtgacaac cctgatcgga caagggatta
     5161 attcttttac aaccttacaa cctattttaa tgtctatttt aattgcagtt gctttctcgc
     5221 ttatcattgt tagtccgatt tcaactgttg caatcggttt agctattggg cttaatggaa
     5281 tggctgcggg agcagcttct atgggaattg catcgacagc agcagtctta gtgtgggcga
     5341 cgcttaaagt taataaatct ggtgtaccaa ttgctatcgc acttggggct atgaaaatga
     5401 tgatgccaaa cttcttgaaa catcctatca tggctattcc gatggtgttc actgcagcaa
     5461 ttagctcatt aacagtacca ttgtttaatc ttgttggaac accagcttca tctggttttg
     5521 gcttagttgg tgcagtaggt ccgattgctt ctttagcagg tggtagctca atacttatta
     5581 ttatccttgc ttggatcatt gttccgtttg cggttgcttt tgcggcgcat aaggtttcta
     5641 aagatattct aaaactttat aaagaagaca ttttcgtctt tgaaggctaa taggaggaaa
     5701 atatgttagt ttatattgct ggctcaggag ccatggggtg ccgttttggt taccaaattt
     5761 caaaaacaaa taacgatgtg attttgttag ataactggga agatcatatc aatgccatta
     5821 aagaaaacgg tttagtggtg acaggggatg tggaagaaac agttaagctt cctattatga
     5881 aaccgactga agccactcaa gaagccgatt tgattatctt gtttacaaaa gcaatgcagt
     5941 tgccacaaat gcttcaagac cttaaaggca ttattggaaa agaaaccaaa gtgctttgcc
     6001 ttttaaatgg tcttggacac gaagacgtta ttcgtcagta cattccagag cacaacattt
     6061 tgatgggggt cactgtctgg actgctggtc tagaaggacc aggtcgtgct catcttcaag
     6121 gtgtgggagc acttaactta caaagcatgg atccaagcaa tcaagaagct ggacaccaag
     6181 tggctgattt gttaaatgaa gctaacttga atgcgactta tgatgagaat gttgtgccaa
     6241 acatctggcg aaaagcttgc gtgaatggta ccatgaactc aacttgtgcc cttctagatt
     6301 gtacaattgg tgaattattt gccagtgaag acggtctcaa aatggtaaaa gaaatcatcc
     6361 atgagtttgt tatcgttggt caagcagaag gtgtcgaact aaacgaagaa gaaatcacac
     6421 aatatgtaat ggatacatct gttaaagctg ctcaccatta cccatcaatg caccaagacc
     6481 ttgtgcaaaa tcaccgattg actgaaattg attttatcaa tggtgcagtc aataccaaag
     6541 gtgaaaagct tggtatcaat actccatact gccgtatgat tacagagcta gttcacgcta
     6601 aagaagcagt gttgaacatc caataaacgc tatcaaatcg ttatactcaa tattatggct
     6661 aaagctttaa tattgagttt tttgttatgc tgatagtatt tttcttaggc gtcattattt
     6721 gtgagcctag attatgagtt aataaaagaa ataataattg gtagaatttg atagattttg
     6781 aaaggttatg cttgactttt agttataaaa tagtataata attgtaaacg atttcagtta
     6841 ggggtggttc tatcttaaaa tcaaaacgaa aacaattaat catggcaaag atcactgaag
     6901 aaaactatgt ttctttagag gatttaatgc aattactcaa ctcctctgag tctactatcc
     6961 gtagagatct aggagagttg gagcaagaag gacggctaca tcgtgttcat ggaggtgctg
     7021 aactttttca ctctttacaa gaggaacttt cgaaccaaga aaagtctgtc aaaaacagtc
     7081 atattaaaaa agctattgcg caaagagcct ctcagtttat ctatgacaac gatgtgatct
     7141 ttattgatgc tgggacaacg actgaatttt tattaccttt tttacaagct aaaaatttaa
     7201 ctgtagttac taattctatt catcatgctg ctagattagt tgaactctct attgagacga
     7261 ttattgtagg gggttatgtc aaacaaacga ccgatgccag tattggtaat gtagctttgg
     7321 aacagattag gcagatgaat tttgacaagg catttcttgg aatgaatggt gttgatgatt
     7381 cttacctgac gactcctgat atggaagaag cagttattaa aaaagcggtt ctgtcaaatg
     7441 caaaattagc ttacatttta gtagatggca ctaaaattgg tcaggtatcg tttgtaaaag
     7501 tagctcctat taatgatgtg actattatta cattaggagg atcagcaagt attcttaaac
     7561 aaataaagga aaaggcaaag gtgattgaat tatgatttat accgtgacct taaacccttc
     7621 tattgacttt attgtcagaa ttgaccaaat aaatcttggg tcagtgaatc gcatggcgag
     7681 tgatgataag tttgctggag gtaaaggcat taatgtgagt cgtatcttgc aacgtttaga
     7741 cattgctagc acggcgacag ggttcttggg tggttttaca ggacgtttta tcgaagaaag
     7801 tttgaatgca gaaggcgtta aaactgattt tgtaaaaggt gatcaagata ctcgaatcaa
     7861 tgttaaaatc aagtcacaag aagagactga aataaatggt caaggacctg tcattagtcg
     7921 ggaacaacta gaggatttga aaactaagct ttcccagcta acagcagagg atacagttgt
     7981 ctttgcaggt tcagcgccag ctaatcttgg caatgctgtt tataaggaat tacttccttt
     8041 agttaggcaa agcggtgctc aggtggtttg tgattttgaa ggacagactt taatagatgc
     8101 gcttgcatat aatcctctgt tggttaaacc taataatcat gaactagaag ctatttttgg
     8161 tacgattctg actagtcttg atgatgttga aacctatgcc cgtcgtcttt tagagatggg
     8221 tgctcaaaac gtcatcattt caatggctgg tgatggtgct ttgctggtta ctaaagaagc
     8281 gacttatttt gctaaaccga ttaagggaga agttaaaaat tctgtcggtg ctggtgattc
     8341 gatggttgct ggctttacag gcgaatttat gaagagtcaa aatccaatag aagccttaaa
     8401 atggggcgtg gcttgtggta cagcgacggc tttctctgat gacttggcga caattgcatt
     8461 tattaaggaa acatatcata aagttgaggt agaaaaacga tgaaaattca agatttattg
     8521 agaaaagata ttatgattct cgatttacag gctatttcta aagaagttgc cattgacgag
     8581 atgattacta aattagttga aaaagatatt gtacatgatt ttgatgtctt taaaaagagt
     8641 atcatgacac gtgaagaaca aacatcaaca gggcttggtg atgggattgc catgcctcat
     8701 tctaaaaata ttgtagtaga taaaccagcg gtcttgtttg cgaaatcaaa taaaggtgtg
     8761 gattataagg ctttagatgg tcaaccaacg gatctcttct ttatgattgc tgcgcctcaa
     8821 ggagcaaatg atactcactt ggctgcgcta gcagaattat cacaatacct tttgaaagac
     8881 ggctttgctg ataaacttcg tgctgcagcg acaccagaag cagtcattgc tgtttttgat
     8941 gaagcgtcaa cagctaaaga agaagtggtt gctccaacaa gtggtcaaga ctttatcgtt
     9001 gctgttacag cttgtccgac aggaattgct cacacttata tggcagaaga ggctttgaaa
     9061 aaacaagctg cagaaatggg agtagctatt aaagttgaaa caaacggtgc ttctggtgtg
     9121 gctaatcgtt taaccgctga agatattcag agagctaaag gagttattgt tgcagctgat
     9181 aaagcggttg aaatggaccg ttttgatggt aaacaattca ttgctcgccc tgttgcagat
     9241 ggtatcaaga aaagtcagga attgatttct ttgattttaa acaatgaagg aaacacttat
     9301 catgctaaaa atggaaaatc tgaagcagca gtatcaactg tgaaaacaag tctaggcggt
     9361 gctttctata aacacttgat gggcggtgtc tcacaaatgt tgccatttgt tattggtggc
     9421 gggattatga ttgctctagc atttttattg gataacatgc ttggtgtgcc aaatgatcag
     9481 cttggaagtc ttggatccta tcatgaaata gcagctattt ttatgaacat tggtggagca
     9541 gccttttcct tcatgttacc agtactagca ggttatatcg cttattctat tgctgaaaaa
     9601 ccgggcttag tagctggttt tgtggcaggt gccattgcct caaatggtct tgcttttggt
     9661 aaggtaccat ttgcagcagg aggagaagtg agcttgggct taacgggcgt gccatcaggt
     9721 ttccttgggg cgcttgtggg tggtttcctt gccggtggtg ttattcttgc ccttcgtaaa
     9781 ttgttggcag gcctaccacg ttctctagaa ggggttaaat ctatcctcct ttacccatta
     9841 cttggcgtcc ttgtcactgg tttcttgatg ctttttgtca atattccaat ggcagctatt
     9901 aacacagccc ttaatgattt cttacaaggt ctttcaggaa gctctgctgt ccttatggga
     9961 cttcttgttg gtggaatgat ggctgttgac atgggtggtc ctgttaataa ggcagcttat
    10021 gtttttggta caggaacttt agcagctact gtggcaaatg gtggttcggt cgttatggcc
    10081 gctgttatgg caggtggtat ggtacctcct cttgctgttt ttgtagccac tctcttgttt
    10141 aaagataaat ttaccaaaga agaacgtgaa tcaggcttga caaatattgt tatgggactt
    10201 tcattcatca ccgaaggagc aattccgttt ggtgcagctg acccagcacg tgcgattcct
    10261 agctttatcg caggttctgc cttgacagga gctcttgttg gtttggctgg tattaaatta
    10321 atggcgccac atggtggtat cttcgtaatc gctttaacaa gcaatccaat cttatatctt
    10381 gtctttgttg tgattggcgc ccttgtatca ggtatcttat ttggcgctct tcgcaaaaaa
    10441 gcctaaaagg taaagaactt ttttcttact aaaactagaa tctgtcaagg ttctagtttt
    10501 ttgctatact agagttaaag gataagaaac gaggaccttt gaaggtgaaa aaaaggagaa
    10561 gaagggctaa atcatcagta aatcgacttg tcttagggtt agtattactt aatctgattg
    10621 ttagtatgtg gacactaaaa ttgggaaacc agcgattagc tccgtatgcg gatcacgaaa
    10681 ctctgacatt tgtcaggaag attagtcatg ctgctcaatc agtcgctcaa aagaaacagt
    10741 tatacagttc ggtgatgatg gctcaggcca ttttagaatc caataatggc aagtcacaac
    10801 taagtcaaaa accttattat aattttttcg ggattaaggg gagctataaa gaacggtcag
    10861 tcatttttcc aactttagaa gacgatggac aagggaatct ttatcaaatt gatgctgctt
    10921 ttcgttccta tgggagcctg acagcttgtt ttttagatta cgcaagagtt ttgaatgatt
    10981 cactttatga caaaacccac aaaaagtttt ggtctcatta tcaagatgct actgcaacct
    11041 taacaggcac ttacgccact gatacaactt accataccaa attaaatgaa ttgattgaat
    11101 ggtatcaact cactaatttt gatggtctaa tgaaatagtt atccacaatt tttaaaaaaa
    11161 tcagaagaaa tatgtcgatt tcaagacaaa agttatcaat ctcattaaat tatcctgtgg
    11221 aaaagttaat ttaaacgttc gtgtgctata atagaaacat agaaaatgaa taaaaaagga
    11281 tatgtgaata atgacaaaaa agaaaggtaa gcttgtactt atcagtcttt ttgttctagc
    11341 agcttgttta ggagcttata gtgcaatgag acaatctcat aaaacttcaa acgtgtcagc
    11401 tgagactatc gccagttctt caacccgaca ttttattgat gaaattggtc caactgctag
    11461 tactattggt caagaacgtg atctttatgc gtctgttatg atagcacaag cgattttaga
    11521 atcaagtaat ggtaaatcaa gtctaagtca agcaccttat tataattttt tcggtattaa
    11581 gggtgcctat aacggttctt ctgtgacgat gtcaacttgg gaagatgatg gtaatggcaa
    11641 tacctatacg attgatcaag cctttagagc ttatccaagt attgcagact cccttaatga
    11701 ctatgcagac ttgctaagtt ctagcactta tataggtgct agaaaatcaa acacgctatc
    11761 gtatcaagac gctacagcag ctctaactgg cttatacgct acagacacca gttataactt
    11821 aaaactaaat aatattattg caacctatgg tttgacggct tatgatgttg ctaatagctc
    11881 agctcaagaa actggtctag caacttcggg ttatgtttgg aatgagtatc gtcgtaatta
    11941 cactgatgcg gagaccctag cagttgatga ggcttgggct aaacgcatga cttattaatg
    12001 taacactaac aacaatctgc ttgcactgac cccaaaaaag ctggatactc tattttaagt
    12061 aaaggattta gttctgtatt gtacagaact aaatcctttt agttttactt tgatatgttt
    12121 atcgttgtaa taatcaatgt catttgtaat cgcttgttct aagtcttcta gtgatttata
    12181 gttattctca taaccataga acatttctga ctttaagatg ccaaagaaag attccatcat
    12241 gctattgtta ggactgtttc ttttacgtga catagacgga cgaattcctt tagagtttag
    12301 gaaatgatga taatagttgt gttgatattg ccaaccttga tcactctatc atatagttcc
    12361 atcatagcat ttatttggag atgtcttttt ccattattgg tttaatgtga tttaagcaag
    12421 gttattagct aaacttaatg ccagacaaaa acaccccaat tattagattt tttgtctaac
    12481 ttttggggcg caattcaatt tgagcagttt tagatttggt atcaatgtta ttataggtca
    12541 attgatcata tttatccaaa gcttttagct tatttagtcc aaagataaat atcaaaatgg
    12601 ctgaaatctt tcatgtttat agtcttattg tctttatatt ttttaaagac atcagatctg
    12661 gtgctattgg gttttgcgtc atataaattg attttttcat gtttcctatt tttactgtga
    12721 atttctaaac tcccccttat ataaggagac ttagtcaaat gaatttcttt tgattccatc
    12781 aaaaattttc ttattttaaa atcgaactct tgaatcgtaa caatatcctt atcgattttt
    12841 ataggattta ttaatgtttt ttgctgttgc ccatctataa aaaaattccc tgcaatagta
    12901 ttagtcctta gaataccatc atctgttgaa tgtaatcttt cttattgata cgctttaatt
    12961 ggtctgattc caactatctt gccctgttga aagtgtaaat aaccctagta cttgagcacc
    13021 aatattatct tcttttattt ctttagcaga aatagctact tttccagcgg aattaacacc
    13081 aacaaagtat ttcttcatac caatagatgc attactatca gagtctgtta cataaatagc
    13141 tttaaggttc ccgttagaat caaagtcagc tccccacagg tttataacat ggttgatgcg
    13201 tacgttagcg taggtgtgtg ataggcctag agccttgcct tcggttaact ctttcttaat
    13261 gagatcactg atttctttga gatttttttc tttaaaatca tgacgacttg tcaatagctt
    13321 actttgatca cctcttgtaa atacggcgtc aaaaatacca cctcggcgat ctttctcttg
    13381 ataggttcta ttgacatcag tagtctgtgt cttataaaca tttaagtaat aaccattgat
    13441 aaacatatct aaaacaagat caggcataac tccaattcgg cgtgctgaca aaccggggaa
    13501 agctttatct cggaaataat taaaaagctc gctatttgtt tggtcacctt tggtattaat
    13561 aacttttctt acatccaata attcttgatc accaaacatg attttttgtt tatctgggtg
    13621 tttttttaga tatgcttcaa ttttttcttt gttttgatcg aaccaccagt gaagcatatt
    13681 ccctgctgtg gcagccccgc aaagaagatc gtcttttcca ttgaatgttt tggtaatatc
    13741 ataccatcct tggttagcaa cataaggagc gtgaaaaaca tcttcgcctt gagtgaattt
    13801 tgctggagga gtaactcctt tggtctaaac ggaagtaacg tgataaggtg ttacttccga
    13861 atatctaatc tcttgattag cagaaaaact atctgctata acaccacgat ctaccgatag
    13921 agcaaataaa gtcactgctg ccaatactgc agctgaagtt gaatagcatc tttttctcat
    13981 aagaatacct ttttctatat aattttatga taaagaaatt atataatata gacatatatt
    14041 attgcaagtt tttaaaataa tttttaataa aattaaacaa gcgattgaca atggaga
//
LOCUS       DAUCWU010000038        14074 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUCWU010000038 DAUCWU010000000
VERSION     DAUCWU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.038"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..2187
                     /gene="recJ"
                     /locus_tag="VGY24_001051"
     CDS             <1..2187
                     /gene="recJ"
                     /locus_tag="VGY24_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ4284034.1"
                     /translation="KIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTEEALDEFLT
                     ADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVKETLDMMGA
                     EPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQEQEVDVIV
                     TDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPTDCLDLVAI
                     GTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDAIGFQIAPQ
                     LNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQAMAMVDPQK
                     PVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAINIFEALNG
                     KRELFTAFGGHAGAAGMTLPVDNLEALSDLLCQFVIERGLDQTAKNTLTIDERLSLDD
                     LSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQGKASFDVL
                     AFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLDLRTKTAKV
                     PEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPYYLTGFGSR
                     EQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVTIDDGLMTV
                     NPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            2337..2855
                     /locus_tag="VGY24_001052"
     CDS             2337..2855
                     /locus_tag="VGY24_001052"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ4284035.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            2936..3619
                     /locus_tag="VGY24_001053"
     CDS             2936..3619
                     /locus_tag="VGY24_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ4284036.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            3616..4272
                     /gene="nth"
                     /locus_tag="VGY24_001054"
     CDS             3616..4272
                     /gene="nth"
                     /locus_tag="VGY24_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ4284037.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            4344..5030
                     /locus_tag="VGY24_001055"
     CDS             4344..5030
                     /locus_tag="VGY24_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ4284038.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            5020..5808
                     /locus_tag="VGY24_001056"
     CDS             5020..5808
                     /locus_tag="VGY24_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ4284039.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMERDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPSGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            5848..6954
                     /locus_tag="VGY24_001057"
     CDS             5848..6954
                     /locus_tag="VGY24_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ4284040.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KTSGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            7013..7882
                     /gene="rfbA"
                     /locus_tag="VGY24_001058"
     CDS             7013..7882
                     /gene="rfbA"
                     /locus_tag="VGY24_001058"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ4284041.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            7882..8475
                     /locus_tag="VGY24_001059"
     CDS             7882..8475
                     /locus_tag="VGY24_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ4284042.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            8719..9759
                     /gene="rfbB"
                     /locus_tag="VGY24_001060"
     CDS             8719..9759
                     /gene="rfbB"
                     /locus_tag="VGY24_001060"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ4284043.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGCIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(9842..10981)
                     /locus_tag="VGY24_001061"
     CDS             complement(9842..10981)
                     /locus_tag="VGY24_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ4284044.1"
                     /translation="MWSEKHKSGKVNFVERYKHPYTGKWCRASVLMEKDTPRIRKEAQ
                     KYLDEKISKILTSLTTTDAYLLDVMNEWWEHHQKSLKSTSVRSLDFRIRELRNLIDPE
                     VMIAKITTKYLQSIIDKIPGSYDKRKRARQLLKQTFDYAIALEYVSINPVISTQLAKP
                     VKTIKDFEDVAQKFLEKDELKRLLDEMYRRKGSIKMAYLAEFMSLNGCRIGEALAIQP
                     DNIKNDIIEIHGTLDYTSNGYRNAIKTTPKTNSSWRETLITKREKEIIQDILKINALE
                     KNTNPNYKDMGYIFISRNGVPIQDNALNTSIRAANKRLEKPIQKELTSHIFRHTLVSR
                     LAENKVPLKTIMDRVGHADSKTTQQIYTHVTKSMKNEVVDILNRL"
     gene            complement(11109..11375)
                     /locus_tag="VGY24_001062"
     CDS             complement(11109..11375)
                     /locus_tag="VGY24_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEQ4284045.1"
                     /translation="MGFLDNVKEASTVSTASGENYEYVVLQVTLKEKLIGTGSGNLTE
                     LENVINKQAKKGYRLHTITTSNGGSKGFGGGDRIQATMVFEKIK"
     gene            complement(11387..11773)
                     /locus_tag="VGY24_001063"
     CDS             complement(11387..11773)
                     /locus_tag="VGY24_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ4284046.1"
                     /translation="MDLSLQEYVKGHGYELLFYDNRGTDKEAFANHDCKVLGIGSYLD
                     DHEKKKAIYHEIGHKEHTNRQYELNRELCELEADRNMIHHLLKEELSYLDNVNEFNYA
                     RFMEKYKLKTIADEKMVIDEYYTLIG"
     gene            complement(11776..12126)
                     /locus_tag="VGY24_001064"
     CDS             complement(11776..12126)
                     /locus_tag="VGY24_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284047.1"
                     /translation="MFPTFEKIKELCKQRGITLIQLEETLGYSRNTLYKLKTQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPAIANGTPSQKTIDFKEIAAQSMSYDGKPLTDDDID
                     LIAAVLEQRFKNRD"
     gene            12422..12592
                     /locus_tag="VGY24_001065"
     CDS             12422..12592
                     /locus_tag="VGY24_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284048.1"
                     /translation="MKKNIVDLQKRKEHFQWVADSLEGKENELYVERDWYDNPTLISK
                     EDAKKEVEQIRQ"
     gene            complement(12618..13406)
                     /locus_tag="VGY24_001066"
     CDS             complement(12618..13406)
                     /locus_tag="VGY24_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284049.1"
                     /translation="MSRYSDYKYANTILKSIEERPEKYLIIHYSCESFYNLGGKSPRI
                     ASISVRQFNNAQTNNFSIHQYSEILDTPITDDTYETIEKELLTDFFTFVDRNTDKNWI
                     HWNMRDTVFGFHAIEQRFKVLGGTPIIIDDDKKIDLAYLFKKMYGGGYIDNPHIEKLL
                     ELNNLTPHRFLTGKQEADAFSSRQYHELSMSTSSKVNSFSTFLTLAINDDLKTNTPKW
                     KMRGTNLSGLLSTFQETISGKVIIGIINLIVGGIVGAIIAKYIQ"
     gene            13457..13648
                     /locus_tag="VGY24_001067"
     CDS             13457..13648
                     /locus_tag="VGY24_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ4284050.1"
                     /translation="MPDIAVGREKVVTFLKSNNIKKSDLAAAYGLNRQEVTNILSGST
                     RGPKANQFILRVIADYSIE"
     gene            13727..14038
                     /locus_tag="VGY24_001068"
     CDS             13727..14038
                     /locus_tag="VGY24_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ4284051.1"
                     /translation="MPKAELVYRPAKQSEKAEAGDYEHLCQIWEGLTVGTAKVWATEM
                     RDHPDFKQFVLNPTHKIVFIDYEGFRLFVKWKSRNRYKPKKETLVEMLENIKFEKRVG
                     V"
BASE COUNT         4274 a         2460 c         2979 g         4361 t
ORIGIN      
        1 aaaataaaag acaagaagcc agatgatggc ttctttaagc tagcaaagac aaaaggtcta
       61 acccagaccg ccgctcagtt gatttatgat cgagggataa ggactgaaga agccttagat
      121 gagtttttaa cagcggactt gtctcaactg catgatcctt acttgctaca tgacatggca
      181 aaagctgtgc caagaattcg tcaagctatt gaagagggcg aacgaattct catttacggc
      241 gactatgatg ctgatggcat gacgtcggct tctattgtca aagaaaccct agacatgatg
      301 ggagcagagc ctttggttta tcttcccaac cgctttacag atggctatgg ccccaaccag
      361 agcgtctata aatattttat tgagcaggaa gctgtgtccc taattatcac ggttgataat
      421 ggcgtggccg gacatgaagc gattagatat gctcaagaac aggaagttga tgtcattgta
      481 actgaccatc atagcctgcc tgaagaattg ccagaagcct ttgccatcat tcatcctgag
      541 catcctgatg ctgattatcc ctttaaacac ttagctggct gtggcgttgc ctttaaattg
      601 gcaacagctt tgctagaaag tttaccaaca gactgtctag acttggtagc tattggaacc
      661 attgccgata tggtaagctt gacaggtgaa aaccgtgttt tagtcaaaaa tggtctggcc
      721 atgctcaaac acaccgaaag agtgggcttg caggagttga tgtccttgtc tcctattgat
      781 ttggaacatt ttaatgaaga cgccattggc tttcagattg ctccgcaatt aaatgcttta
      841 gggcgcttag atgaccctaa tccagccatt gagctactga cgggctttga tgaccaagaa
      901 gctcaagcga ttgccttgat gattaagaaa aaaaatgaag agcgtaaggc cttagtccaa
      961 gacatttttg atcaagcgat ggctatggtt gatcctcaaa aacctgttca agtcttggca
     1021 caagctggct ggcatcctgg tgttcttggg attgtcgctg gtcgtatcat ggaaactatt
     1081 ggacaaacgg tcgtcgtttt gaccattgat aatggctttg caaaaggctc ggccagaagt
     1141 ttagaagcta ttaatatttt tgaagccttg aatggcaagc gtgagttatt tacagccttt
     1201 gggggacatg ctggtgcagc aggaatgacc ctgcccgttg acaatttgga agctctctca
     1261 gacttgctct gtcagtttgt gatagaacgt ggcttagatc agactgccaa aaatacgtta
     1321 actattgatg aaagactgtc tttggacgac cttagccttg atattttaaa gagtttagat
     1381 aaattagccc cttatgggat ggatcatcaa aagccggtct tttatgtcaa ggacataagg
     1441 gttagtcaag cgcgtaccat agggcaagac cagagccatc ttaaatttaa agtcagtcag
     1501 ggcaaagctt cctttgacgt actggctttt ggtcaaggaa gtcaactgca agagttccga
     1561 caagctactg gtttagaatt agcagttacc ttatctgtca accattggaa tgggaacacg
     1621 agtctccaat tcatgttagt agatgctcgt gttgacggag tgcaacttct tgacttaagg
     1681 acaaaaacag ccaaagttcc tgaaggtatt ccgactattg aagaagaccc aaatgccaga
     1741 gtcattctga ttaatgacat ccctgaagat ttcaaaacct ggcgcaacca gtttgttcac
     1801 aaggattttg acgccatcta ttttaaaaat cagatgaaac atccttatta tttaacagga
     1861 tttggcagca gagagcagtt tgccaagctc tacaaaacca tctatcagtt tcctgaattt
     1921 gatttacgac ataaattgac agagttgagt cactatttaa acatagaaaa acttttactg
     1981 attaagttga ttcaaatttt tgaagagttg tcttttgtga cgattgacga tgggctgatg
     2041 acggttaacc cccaagcgca aaaacgagag atttcagaga gccacattta ccaagactta
     2101 aaagaattgg tgaaatttca agaaatcatg gctttggcta gcccgaaaga gatgtatgat
     2161 tatttggtga aatcaaacga tcaataagta aagcagttga cagtagttat tgaggtagtt
     2221 acgaatgaag tatcagataa acataatgtc agaagtctta ttttacgaaa aatatgtttc
     2281 gtgatataat gaagagtatt ttatatttgg caatttgcca ctagaaagaa ttaactatgg
     2341 atttaacaaa ttacattgca tcgatcaaag attaccctaa agctggtatt acgtttcgag
     2401 atatctcacc tttaatggct gacggtaaag catatagcta tgctattcgt gaaatagcac
     2461 agtacgcttg tgataaagat attgatatgg ttgttggacc tgaagcgcgt ggttttatca
     2521 ttggttgtcc tgttgcagtt gagttaggaa ttggttttgc tcccgttcgt aaaccaggaa
     2581 aactgccaag agatgttgtc tctgctgatt acgaaaaaga atatggtctt gataccttaa
     2641 ctatgcatgc agatgctatt aagcctggac aacgtgtttt gattgtcgat gatttactag
     2701 cgactggcgg tacggtaaaa gcaactattg aaatgattga aaaacttggt ggtattgtag
     2761 ctggttgtgc cttccttatt gaattagaag gtttaaatgg acgccatgct attaggaact
     2821 atgactataa agtcttaatg cagtttcctg ggtaagcaca ttaatcagca tctgattagg
     2881 tgatttaaag tagtaaaggc tatagattcg attttagcct tttgattgag ggattatgag
     2941 ttttttagaa cattataagt caggtaattt ggtcatccca agtgccttgc tttttcatta
     3001 caaagatctt tttaagagct cagatgattt tttggtctgg cagtttttct atcttcaaaa
     3061 tacgacaaag cgagacgact tagcgcccag tcaaattgct catgcgcttg gaaaatcagt
     3121 tgctgatatt aataaaatca tttcatcttt gactagtcaa gggcttttag atatgcggac
     3181 aattgaactt actggagaaa ttgaaattat ttttgatgcg agtcctgtgt tagccaagtt
     3241 agatcaattg tttgtcagtc aaacggcaac tgaaattgac aaacaagaaa caccaaatca
     3301 tttcaaacga ttagttgatg aatttgaacg tgaactagga cgattcttga gtccttttga
     3361 gttagaagat cttgaaaaaa cgcttcgtga tgataaaaca gatcctgatt tgatacgaga
     3421 agctttaaaa gaagctgtct tcaacggcaa aacaaattgg aaatatatcc aggctatctt
     3481 acgtaattgg cgaaaagaag gtattgttaa tcttcgacaa gtagaagaac gtagacgtgt
     3541 tagagaaggc gaagatcttt ctcaagtgac gatatcagag gattttctat ctgcgatgaa
     3601 tttatggagt gattcatgag aattggaaaa gcaagattag caaaagtcct gaccatcatt
     3661 ggtcaaatgt ttcctgaggc taagggagag ttagactggg aaacaccttt tcaattgtta
     3721 attgcggtga ttttatcagc tcaaacgaca gataaggccg tcaataaggt aactcctgga
     3781 ctatggcagt cttatccaga aatagaagac ttagcttttg ctgaactttc tgatgttgaa
     3841 aatgctctga gaacgattgg actctataaa aataaggcca aaaatatcat taaaactgct
     3901 caggctattc gtgatgattt taagggtcaa gtgccaaaaa cccacaaaga gcttgaaagt
     3961 ttaccgggtg ttggccgaaa aacagccaat gtggtattgg cagaggttta tggtgttcca
     4021 gctattgcgg ttgatactca tgtggctaga gtctcaaaga ggctcaatat ttcatcacca
     4081 gatgccgatg tcaagcagat tgaagcagat ttgatggcta aaattccaaa gaaagattgg
     4141 attattactc accatcgatt gattttcttt ggacgctacc attgtttagc caaaaaacca
     4201 aaatgtgaga tttgtcccgt tcagtcttac tgtaagtact atcaaaatac ttatggaaaa
     4261 tcgaaagcct agcctttggt tttctttttt tatggaggta attttgctat aatagaacca
     4321 ttacgtttta gaaagaaatc aagatggata gtcaattatc aaaccgttta gcccaagttg
     4381 cggcctatgt tccaaaaggt gtcaaattat tagatgttgg cagtgatcat gcctatcttc
     4441 ccatattttt ggtagaaact aatcagattt cagcagccat tgctggcgaa gtggttaggg
     4501 gtccctacga atctgctctt aaaaatgtca cacagtctgg ccttgccgag catattcagg
     4561 tgcgcttagc caatggcctt gcagcctttg aagaagctga tgatgtgaca gccattacca
     4621 tttgtggcat gggtggccgt ttgattgctg atattttaga ggcaggtaag gaaaaattgc
     4681 agggcattga acgtctggtt ttacagccca acaatcgtga ggatgattta cgggcttggt
     4741 tatcggttaa cgcttttaaa attgttgcag aaaccatcat ggctgaaaat gacaaatatt
     4801 atgaaatcat cgttgctgag catggtgaaa aggccttatc cgcgacagaa ttgcgttttg
     4861 gtccatacct ttctcaggaa aaatcagtcg tttttaaaga gaagtggcaa cgagaaatgg
     4921 ataagttagc ttatgccttg tcctgcatcc cagaagaaaa aactcaagag cgtcagcttc
     4981 tcttgacaaa aatacaacaa ataaaagagg tgattgttca tgaaagctaa aaccctaatt
     5041 gatgcctatg aagccttttg cccacttgat ttatctatgg aaagagacgt taaaggtctt
     5101 caaatgggtt cattggataa agacattcgt aaggttatga ttactttaga tatccgagag
     5161 tcaacagtag cagaagccat caaaaatgaa gttgatctca ttataactaa gcatgctccc
     5221 atctttaaac cgcttaaaga tctggtttca tcccctcagc gtgatatcct tctagatttg
     5281 gtcaagcatg acatttctgt ctatgtgagc cataccaata ttgacattgt accaagcggg
     5341 ctaaatgatt ggttttgtga cctgctagaa atcaaggaag caacttactt gtctgaaacc
     5401 aaagaaggct ttggcattgg tcgtattgga acagttaaag aacaagcgtt agaagagtta
     5461 gcaagtaagg tcaaaagggt gtttgattta gataccgtgc gacttatccg ttacgataag
     5521 gaaaaccctt tgatttcaaa gattgctatc tgtggcggaa gtggtggtga attttatcag
     5581 gatgctgtgc aaaaaggcgc agacgtttat attacagggg atatctatta ccatacagca
     5641 caggagatgc tgacagaagg actatttgcg gttgacccgg ggcatcatat tgaggtgctt
     5701 tttacggaga aattaaagga gaaactccaa gggtggaaag aagagaacgg ttgggatgtc
     5761 agcattattt caagcaaagc ctcgaccaat ccatttagcc atttataggc tgttaaggtc
     5821 ttaaagtaag ataaaaggat accaggaatg aaaatagcca ttattggggc ggggattgta
     5881 ggatcaaccg ccgcttatta tttacaacaa agtggtcaaa aagaagttac catttttgac
     5941 catggtcaag gacaagccac taaggctgct gctggtatta ttagtccttg gttttcaaag
     6001 cgtcgcaata aagtttggta ccgcatggct cgtttaggag cggactttta tcagcaatta
     6061 atcaatgacc tcaaagaaga tggttttgca acagattttt atcagcaaaa tggcatctat
     6121 gttttaaaaa agcaggaaga aaaactaaga gatctttatg aattagcgtt agcaagaaag
     6181 gtagaatcac cgattattgg tgagttagcc attaaaaatc gtaaagaact ggggaatgat
     6241 tttaaaggat tgattggctt tgataactgt ctgtacgctt caggagctgc tagagtggag
     6301 ggagccgctt tatgtgagac gttactcaag actagtggtt atccagttat tcgccaaaaa
     6361 gtcactctga aacagcaagg aagtggttat gagattgctg gtcattattt tgatcaggtc
     6421 attctggcag caggagcatg gctacctgac ttattgatgc ctttaggcta tcaggttgat
     6481 gtgcgaccac aaaagggaca actcttggat tatgacgttc atcacatcat cagcgacaat
     6541 tatcccgttg ttatgccaga aggtgaaata gacctcattc ctttcaatca aggtaagatt
     6601 tctgttggtg cgagccatga aaatgataaa ggttatgatt tggagcctga ttggcaggtc
     6661 ttagaaaaac ttgaaatgca agctctcact tatttgccgt tattaaaaga agctactcaa
     6721 aaatcatgtc gtgtagggat aagagcttat accagtgatt attctccttt ttatggtcag
     6781 gttccaggat tgaagaacct ttatacagct agtggtttag ggtcatcggg attaacagtt
     6841 ggccctttga ttggctatga attagctcaa ttattgctag gacacgaagg acttttaacg
     6901 cctagcgact attcccctga gccttacctt ttgcggtcta ctggtaaagc gtgacactgc
     6961 tttcaaaaaa tgatagaatg atagggtata tagaagaata ggagcactac gtatgaaagg
     7021 tattattctt gcaggaggtt cagggacacg cttgtatcca ctgacccgtg ccgcatctaa
     7081 acagttgatg cccatttatg acaaaccaat gatttattac ccattgtcaa ccttgatgtt
     7141 ggcagggatt aaagatattt taatcatttc aaccccccaa gatcttcctc gtttcgaaga
     7201 attgctagga gatgggtcag aatttggcat tagcttatcc tataaggaac aaccaagccc
     7261 agatggttta gcacaagctt tcattattgg agaggagttt atcggtgatg accgtgttgc
     7321 tcttatttta ggagataata tttaccacgg taatggcctc actaaaatgc ttcaaaaggc
     7381 agctgccaaa gaaaaaggag ccaccgtttt tggttatcaa gtgaaagacc ctgagcgctt
     7441 tggagtggtg gaatttgatg agaacatgaa tgctatctct atcgaagaaa aaccagaagt
     7501 acctaagtcc cactttgcag tgactggact ttatttctat gacaatgacg tggtagagat
     7561 tgctaaaaac attaaaccaa gcgcgcgtgg tgagttggaa attacagacg tcaacaaggc
     7621 ttacttggat cgtggtgacc tctccgttga attgatgggg cgtggttttg catggctaga
     7681 cactggaacg catgaaagtc tacttgaagc ggctcaatat attgaaactg ttcaacgctt
     7741 gcaaaacgct caagtggcaa atctggaaga aattgcctat cgcatgggtt atatcagtaa
     7801 agaagatgtc cataaattgg cgcaatcttt aaagaaaaac gaatacgggc aatacttgct
     7861 tcgtttgatt ggagaagctt aatgacagaa actttttttg acaaaccatt ggcttgccga
     7921 gaaatcaaag aaatcccagg cttactagag tttgatattc ctgttcgtgg ggataatcgt
     7981 ggctggttta aagaaaattt ccaaaaagaa aaaatgttgc caattggctt tccagaacgt
     8041 ttttttgagg aaggaaaact acaaaataac gtttcttttt cacgtcagca tgtgctgcgt
     8101 ggacttcatg ctgaaccttg ggataaatac atctcagttg ctgatgatgg caaggtttta
     8161 ggagcatggg ttgatcttcg tgagggagag acttttggaa acgtctacca gacagtgatt
     8221 gatgcttcaa aaggaatgtt tgttcctaga ggggttgcta atggctttca agttctttca
     8281 gagactgtct cttacagtta tcttgtcaat gactactggg ctcttgactt gaaacctaag
     8341 tatgcttttg taaactatgc tgacccaagt cttgggatta cttgggaaaa tctagcagct
     8401 gcagaagttt ctgaagcaga taaaaaccat cctcttctta gtgatgtcaa accactgaaa
     8461 ccaaaagacc tatagggaaa caacatactc agatgattgg gattaatcat ttgaggtgga
     8521 tatgagagga gttattcttc tctacttcag gtaacaagta acaactaaaa atgatgaaag
     8581 gaaagttcag tttctctctt tgagctgaac atgggactca aatcttagaa agcaatgttt
     8641 tcttaggcct aaaacgtctt tgttagagtc attactttta atagattttt tcggaaacga
     8701 aaggtccctt tatatcttat gtataaaaat attatcgtaa ctggtggagc tggtttcatc
     8761 ggatctaact ttgtgcacta tgtctacaat aaccacccag atgttcatgt aactgtcctt
     8821 gataaattga catatgcagg taaccgtgct aacattgaag ctattcttgg tgatcgtgtt
     8881 gagttagttg ttggtgatat cgctgacgct gaattggtag ataaattggc tgccaaaacg
     8941 gatgctattg ttcactatgc ggctgagagc cacaacgata actcattgga agatccaagt
     9001 ccatttatcc atacaaactt tatcggaact tacactttgc ttgaagcagc tcgtaaatac
     9061 gatatccgtt tccaccacgt gtcaactgat gaagtttatg gagatcttcc acttcgtgaa
     9121 gaccttccag gacagggtga aggaccaggt gaaaaattca ctgctgaaac aaaatacaat
     9181 ccatcatcac cttactcatc aactaaggca gcttctgacc ttatcgttaa ggcatgggta
     9241 cgttccttcg gtgtgaaagc gaccatttcg aactgttcaa ataactatgg accataccaa
     9301 cacatcgaaa aattcattcc acgtcaaatt acaaatatct tggcaggaat caaaccaaaa
     9361 ctttatggtg aaggtaaaaa cgtccgtgac tggattcata ctaatgatca ttctacagga
     9421 gtatgggcta ttttgactaa gggttgtatc ggtgaaacat accttattgg tgccgacggc
     9481 gagaaaaaca acaaggaagt tcttgagctt atccttgaga aaatgggtca accaaaagat
     9541 gcttatgatc acgtaactga ccgtgctggt cacgatcttc gttacgctat tgattctaca
     9601 aaattgcgtg aagaactcgg ctgggaacca caatttacaa acttttcaga aggtttggaa
     9661 gaaactatta agtggtatac agaaaatgag acatggtgga aagcagaaaa agatgctgta
     9721 gaagccaagt atgctaaaac tcaagaagtg attaaataaa acattaaaga accttgtcat
     9781 atcaacgttt gttgaagatg tcaaggtttt ttcttgtgtt ttggggcatt tttggggcat
     9841 attatagccg atttaagatg tcgacgactt catttttcat gcttttcgtg acgtgggtat
     9901 agatttgttg agtagttttc gagtcagcat gtccaactct atccatgata gtttttaagg
     9961 gcactttgtt ttctgcaagg cgactcacca gggtatgacg gaagatgtgg ctggtgagtt
    10021 ctttttgaat cggtttttct agtctcttat ttgccgctct gatagaggtg ttgagggcgt
    10081 tgtcttgaat aggaacacca tttctggaaa tgaagatgta gcccatgtcc ttgtaattag
    10141 gattagtatt tttttcaaga gcattaattt tcagtatatc ctgaataatt tctttttcac
    10201 gtttggttat caatgtttca cgccaactgg aatttgtttt aggggtggtt ttgatagcgt
    10261 ttctatagcc atttgaggtg tagtcaagag ttccgtggat ttcaatgata tcatttttga
    10321 tattgtctgg ctgtatagct agagcttctc caatgcggca gccatttaag ctcataaatt
    10381 ctgccagata agccatttta atcgatcctt tacgtctata catttcatct aatagccttt
    10441 tgagttcatc tttttctaaa aacttctggg caacgtcttc aaagtctttg atggtcttaa
    10501 caggttttgc gagttgggta ctaataactg gattgattga gacatactct aaagcgatag
    10561 cgtagtcgaa ggtttgtttg agcagctgcc gtgctcgttt acgtttgtca taactacctg
    10621 gtattttgtc gataatactt tgcaggtatt tcgtggttat cttggcaatc atcacttctg
    10681 gatcaattaa gtttcgcaat tctcttattc taaaatcaag agagcggaca gaagttgatt
    10741 tgagtgattt ctgatgatgt tcccaccatt cgttcatgac atctagtagg taggcatcgg
    10801 ttgtggtaag acttgttaag attttagata ttttttcgtc aagatatttt tgggcttctt
    10861 ttcgtattcg tggcgtgtct ttttccataa ggacagaagc tctgcaccac ttgccagtat
    10921 acgggtgttt gtaccgttct acaaaattta cttttccgct tttgtgtttt tctgaccaca
    10981 ttgtttttta cctcattttc tgttaaaata ggtatagtaa agagacctac tgcgaagcag
    11041 gtttttacta tactagattt gcctcacgct cgcaccgacc aaaatttgag cgtagggctt
    11101 tttttatttt acttgatttt ctcaaatacc attgtagctt gtattctatc tccgccacca
    11161 aatcctttac tgccaccatt tgatgtggta atagtgtgta ggcgatatcc tttttttgct
    11221 tgcttattaa taacattttc aagttcagtt aaatttcctg agcctgttcc tataagcttt
    11281 tcttttagtg taacttgtag cacaacatat tcataatttt cgccagaagc agtcgaaaca
    11341 gtactagctt cttttacatt gtccaaaaat cccatgataa tctcctttaa cctattaaag
    11401 tataatattc atcaattacc attttttcat cggcaattgt ttttaactta tatttttcca
    11461 tgaaacgagc atagttgaat tcatttacat tgtctaaata agataactct tcttttagca
    11521 agtgatgaat catatttctg tctgcttcta attcacacag ttctctattt agttcatact
    11581 gtctgttggt atgctcttta tgaccgattt catgatatat tgctttcttt ttttcatgat
    11641 catcaagata agaaccaata ccaagaactt tgcagtcatg gttagcaaac gcttctttat
    11701 ctgtgccacg gttatcataa aataaaagct cataaccgtg acctttgaca tactcttgaa
    11761 gtgataagtc catagttaat ctctattctt gaatctttgt tctaagactg ctgcaatgag
    11821 atcaatatca tcatcggtaa gtggttttcc atcataagac attgactgag cagctatctc
    11881 tttaaagtct attgtttttt gactaggggt gccattagca atagcaggat tatcagtgcg
    11941 accaagcaaa taatctgttg aaacattgaa gtagtctgct atttcagcaa ttctttcagc
    12001 gttgggtttt tgagttttca acttatagag tgtatttcgg ctatatccta aagtttcctc
    12061 tagttgaata agtgtaattc cacgctgctt gcacaattct ttgatttttt caaacgtcgg
    12121 aaacattgat ttatcaacct ttctaaggca tgacaaaaaa tatttcaact ttttgggtga
    12181 aaaatgttga caattcaacc aaatgggtgt atcatatttt ttgtaagcaa gagataacta
    12241 aaaaaacaac taagcaaaca actataaaac agttttggcg aacgagttta agttgatttg
    12301 ttaaggttct ttaatatgct ttaattctaa ctttttaggt gtgagttgtc aagtggtcat
    12361 atactaaaaa gttgaaaatt tagttatttc ttgtttacaa attttaaaac aaggaggaaa
    12421 aatgaaaaaa aacatcgttg atctacaaaa gcgtaaagag cattttcagt gggtcgctga
    12481 tagcttagag gggaaagaaa acgaacttta cgtggaaaga gattggtacg acaatccgac
    12541 tcttatttcc aaagaggatg caaaaaaaga agtagagcaa atacgacagt aacttattct
    12601 acttcaaaat aaatcattta ttgaatatat tttgcaatta ttgcaccaac tattccaccg
    12661 acaataaggt tgattatacc gattatcact tttcctgaga ttgtttcttg aaaagtggaa
    12721 agtaatccag ataggttagt tcctctcatt ttccatttag gggtgttagt ttttaaatca
    12781 tcgttgatag ctaaagtgag gaatgttgaa aagctattga ccttgcttga tgttgacata
    12841 cttagttcat ggtattgtct cgaagagaaa gcgtcagctt cttgtttacc tgtcagaaaa
    12901 cgatgtgggg ttagattgtt caattcaagt aatttttcaa tatgcggatt gtcaatatat
    12961 cctccaccgt acatcttttt aaacagatag gctaaatcga tctttttgtc atcatcaatg
    13021 attattggtg tgccgcctag aactttgaat cgctgctcaa tagcatgaaa gccaaagaca
    13081 gtatctctca tgttccagtg aatccaattt ttgtcggtat ttctgtcaac aaatgtgaag
    13141 aaatctgtta acagctcttt ttcgatagtt tcataagtat catcagtaat tggggtatct
    13201 aatatctcgg agtactgatg aatggaaaag ttgttggttt gggcattatt gaattgacga
    13261 actgaaatgg aagctattct aggactttta ccacctagat tataaaaact ttcgcaggag
    13321 taatggataa tcagatattt ttctggtcgt tcttcaatac ttttaaggat agtattagcg
    13381 tatttataat cagagtatcg agacattttt acctcaagtt tttattttta ttatatcaga
    13441 aaagaaagga gaaaatatgc cagatattgc agttggacga gaaaaagttg ttaccttcct
    13501 aaaaagtaac aatatcaaga agagtgattt agcagcagcc tatggtctca atcgtcaaga
    13561 ggtcactaac attcttagtg gctcaactag aggaccaaag gctaaccagt ttattctgcg
    13621 agtgattgca gattacagca ttgagtaaat gcaaaaaagg aactgattgc agtcagcccc
    13681 ttatcaaaaa ttctacttac attatatcac agattggagg taagaaatgc caaaagctga
    13741 attagtttac agaccagcta agcaatctga aaaagcagaa gctggtgact atgaacacct
    13801 ttgccaaatc tgggaaggtt tgacagtcgg aacggctaag gtttgggcga cagaaatgcg
    13861 tgatcatcca gactttaaac agtttgtatt aaatccaacg cataaaatcg tattcattga
    13921 ttacgaaggt ttccgtttat ttgttaaatg gaaaagtcgt aaccgctata aacccaagaa
    13981 ggaaaccctg gtagaaatgc ttgaaaatat taaattcgaa aaaagagtag gagtataaca
    14041 tgaacaaatt agaattattt ttattagtaa caac
//
LOCUS       DAUCWU010000039        14007 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUCWU010000039 DAUCWU010000000
VERSION     DAUCWU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.039"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            164..1522
                     /locus_tag="VGY24_001069"
     CDS             164..1522
                     /locus_tag="VGY24_001069"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ4284052.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTQATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            1522..2604
                     /locus_tag="VGY24_001070"
     CDS             1522..2604
                     /locus_tag="VGY24_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ4284053.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKNLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            2605..3753
                     /locus_tag="VGY24_001071"
     CDS             2605..3753
                     /locus_tag="VGY24_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ4284054.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMHDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQARG"
     gene            3965..5329
                     /gene="ftsA"
                     /locus_tag="VGY24_001072"
     CDS             3965..5329
                     /gene="ftsA"
                     /locus_tag="VGY24_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ4284055.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            5353..6672
                     /gene="ftsZ"
                     /locus_tag="VGY24_001073"
     CDS             5353..6672
                     /gene="ftsZ"
                     /locus_tag="VGY24_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ4284056.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLEPTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            6675..7346
                     /locus_tag="VGY24_001074"
     CDS             6675..7346
                     /locus_tag="VGY24_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ4284057.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTTDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            7358..8014
                     /locus_tag="VGY24_001075"
     CDS             7358..8014
                     /locus_tag="VGY24_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ4284058.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSSIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            8019..8273
                     /locus_tag="VGY24_001076"
     CDS             8019..8273
                     /locus_tag="VGY24_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ4284059.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            8273..9064
                     /locus_tag="VGY24_001077"
     CDS             8273..9064
                     /locus_tag="VGY24_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ4284060.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRVA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            9074..9832
                     /locus_tag="VGY24_001078"
     CDS             9074..9832
                     /locus_tag="VGY24_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ4284061.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKVKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            10105..12906
                     /locus_tag="VGY24_001079"
     CDS             10105..12906
                     /locus_tag="VGY24_001079"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ4284062.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIAIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLTEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(13171..13473)
                     /locus_tag="VGY24_001080"
     CDS             complement(13171..13473)
                     /locus_tag="VGY24_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ4284063.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(13524..13979)
                     /locus_tag="VGY24_001081"
     CDS             complement(13524..13979)
                     /locus_tag="VGY24_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4284064.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
BASE COUNT         4462 a         2406 c         2996 g         4143 t
ORIGIN      
        1 tacttaaaaa aacgtatcaa taatctcaca aatatgggca tgatgtttgt atattggaaa
       61 cggattctaa ttctaaactc aagaacggta tttttaaaat gttgaaaaag catctaaata
      121 aaggtaaaat agaaatcaaa tagaatggat agtgtgagct tagatgaaag tgataagtaa
      181 ttttcaaaac aaaaaaatat taatattggg gttagccaaa tcgggcgaag cagcagcaaa
      241 attattgacc aaacttggtg ctttagtgac tgttaatgat agtaaaccat ttgaccaaaa
      301 tccagcggca caagccttgt tggaagaggg gattaaggtc atttgtggta gccacccagt
      361 agaattatta gatgagaact ttgagtacat ggttaaaaac cctgggattc cttatgataa
      421 tcctatggtt aaacgcgccc ttgcaaagga aattcccatc ttgactgaag tagaattggc
      481 ttatttcgta tctgaagcgc ctattatcgg gattacagga tcaaacggga agacaaccac
      541 aacgacaatg attgccgatg ttttgaatgc tggcgggcaa tctgcactct tatctggaaa
      601 cattggttat cctgcttcaa aagttgttca aaaagcaatt gctggtgata ctttggtgat
      661 ggaattgtcc tcttttcaat tagtgggagt gaatgctttt cgccctcata ttgctgtcat
      721 cactaattta atgccgactc atctggacta tcatggcagt tttgaggatt atgttgctgc
      781 taaatggatg attcaagctc agatgacaga atcagactac cttattttaa atgctaatca
      841 agagatttca gcaactctag ctaagaccac ccaagcaaca gtgattcctt tttcaactca
      901 aaaagtggtt gatggagctt atctgaagga tggaatactc tattttaaag aacaagcgat
      961 tatagctgca actgacttag gtgtcccagg tagccacaac attgaaaatg ccctagcaac
     1021 tattgcagtt gccaagttat ctggtattgc tgatgatatt attgcccagt gcctttcaca
     1081 ttttggaggc gttaaacatc gtttgcaacg ggttggtcaa atcaaagata ttaccttcta
     1141 caatgacagt aagtcaacca atattttagc cactcaaaaa gctttatcag gttttgataa
     1201 cagtcgcttg attttgattg ctggcggtct agatcgtggc aatgaatttg acgatttggt
     1261 gccagacctt ttaggactta agcagatgat tattttggga gaatccgcag agcgtatgaa
     1321 gcgagctgct aacaaagcag aggtctctta tcttgaagct agaaatgtgg cagaagcaac
     1381 agagcttgct tttaagctgg cccaaacagg cgatactatc ttgcttagcc cagccaatgc
     1441 tagctgggat atgtatccta attttgaggt tcgtggggat gaatttttgg caacctttga
     1501 ttgtttaaga ggagatgcct aatgcctaag aagattttat ttacaggtgg tggaactgta
     1561 ggtcatgtca ccttgaacct cattctcata ccaaaattta tcaaggacgg ttgggaagta
     1621 cattatattg gtgataaaaa tggcattgaa catacagaaa ttgaaaagtc aggccttgac
     1681 gtgacctttc atgctatcgc gacaggcaag cttagacgct atttttcatg gcaaaatcta
     1741 gctgatgttt ttaaggttgc acttggcctc ctacagtctc tctttattgt tgccaagctt
     1801 cgccctcaag cccttttttc caaaggtggt tttgtctcag taccgccagt tgtggctgct
     1861 aaattgcttg gtaaaccagt ctttattcat gaatcagatc ggtcaatggg actagcaaac
     1921 aagattgcct acaaatttgc aactaccatg tataccactt ttgagcagga agaccagttg
     1981 tctaaagtta aacaccttgg agcggtgaca aaggttttca aagatgccaa ccaaatgcct
     2041 gaatcaactc agttagaggc ggtgaaagag tattttagta gagacctaaa aaacctcttg
     2101 tttattggtg gttcggcagg ggcgcatgtg tttaatcagt ttattagtga tcatccagaa
     2161 ttgaagcaac gttataatat catcaatatt acaggagacc ctcaccttaa tgaattgagt
     2221 tctcatctgt atcgagtaga ttatgttacc gatctctacc aacctttgat ggcgatggct
     2281 gaccttgtag tgacaagagg gggctctaat acactttttg agctactggc aatggctaag
     2341 ctacacctca tcgttcctct tggtaaagaa gctagccgtg gcgatcagtt agaaaatgcc
     2401 acttattttg agaagagggg ctacgctaaa caattacagg aacctgattt aactttgcat
     2461 aattttgatc aggcaatggc tgatttgttt gaacatcagg ctgattatga ggctactatg
     2521 ttggcaacta aggagattca gtcaccggac ttcttttatg accttttgag agctgatatt
     2581 agctccgcga ttaaggagaa gtaaatggca aaagataaag agaaacaaag tgatgacaag
     2641 ctcgttttga cagagtggca aaagcgtaac attgaatttt taaagaaaaa gaagcagcaa
     2701 gctgaggaag aaaaaaaact caaagagaaa ttattgagtg ataaaaaagc gcagcagcaa
     2761 gctcaaaatg cttctgaagc agttgagctt aaaactgatg agaaaactga tagtcaggaa
     2821 attgagtcag aaacgacgtc aaaacctaaa aaacccaaaa aagttagaca acccaaggaa
     2881 aaaagcgcga cacaaatcgc ttttcaaaaa tccttgcctg ttcttttggg ggcgctctta
     2941 ctcatggcgg tgtctatttt tatgatcact ccttatagca aaaagaaaga gttttctgta
     3001 agaggaaacc atcaaacgaa ccttgacgaa ttaatcaaag ctagcaaagt caaagcatct
     3061 gactattggt taacgttgtt aacttcgcct ggtcagtatg aacgaccgat tcttcgtact
     3121 attccatggg tgaaatctgt acatctctct taccaatttc ctaatcactt tctatttaac
     3181 gttattgaat ttgaaatcat cgcttatgca caagtcgaaa acggttttca gcctattttg
     3241 gagaatggaa aacgtgtgga caaggtcagg gcatcagaac taccgaaatc tttcttgatt
     3301 cttaatttaa aagatgagaa agcgatccaa cagttagtta agcaattaac gacattacct
     3361 aaaaaattag tcaagaatat caagtcagtg tctcttgcaa attccaaaac gacagcggat
     3421 ttactactta ttgaaatgca tgacggtaat gtagttagag taccgcagtc acaactcaca
     3481 ttgaaacttc cctattatca aaaattgaaa aaaaaccttg aaaatgatag tatagtggat
     3541 atggaagttg gaatttatac tacaacacag gagattgaaa atcaacctga agttcctctt
     3601 acgccagaac aaaacgcagc tgataaagaa ggagataagc ctggtgaaca tcaggaacag
     3661 acagacaatg attcagaaac gccagcaaat cagagtagtc ctcagcaagc accaccatcc
     3721 ccagaaacgg tcctcgaaca ggcccgtggc tagctaatat ttaagttgaa aaagcaatga
     3781 aaacgttaga aattcaacga ttctaaccca taatgaattg cctaaaaaaa attaagttta
     3841 tataacaaaa aacgtaaaat gataacattt tacgtttttt tatggtataa tattttctga
     3901 atgattctgt tttttagcag tttttagaat agcaaaagtt tggaaagtaa gtgaggtcaa
     3961 gtgaatggct agaaatggct tttttactgg tttggacatt ggaacaagct cgataaaagt
     4021 tttagtagca gaatttattt ctggtgagat gaacgtcatt ggtgttagta atgttccaag
     4081 taccggcgta aaagatggca taataatcga tatagaggca gctgcgactg ccatcaaaac
     4141 tgcggtagaa caagcagaag aaaaagcagg gatgacaatt gaaaaggtta atgttgggct
     4201 accggcaaac cttcttcaaa ttgaaccaac acaaggaatg attcctgtcc caagtgagtc
     4261 taaagagata aaagatgagg atgttgatag cgttgttaaa tcggctttaa caaaaagtat
     4321 cacaccagaa cgagaggtta tctctttagt tccagaagag ttcattgtgg atggctttca
     4381 gggcattcga gatccacgtg gtatgatggg gattagatta gagatgcgcg ggcttattta
     4441 tactggacca agcaccattt tacataatct gcgtaaaacg gtagaaagag caggcattaa
     4501 agttgaaaac atcattattt ctccgttagc tatggctaaa accattttaa acgaaggtga
     4561 gcgcgagttt ggagctactg taattgatat gggaggtgga cagacaactg tcgcttctat
     4621 gcgagcacaa gaattgcagt ataccaatat atatgctgaa ggcggcgaat acattactaa
     4681 agatatatca aaagtattaa aaacgtcttt ggctattgca gaagcactta agtttaattt
     4741 tggtcaagcg gagatatcag aagctagtat aactgaaaca gtaaaagttg atgtggtagg
     4801 tagtgaagag cctgttgagg taactgaacg ttatttatct gaaattattt cagcgcgtat
     4861 tcgtcatatt ttagatcgtg tgaagcaaga tttggaaaga ggtcgtttac tagacttacc
     4921 aggaggcatt gttttgattg gtggcggtgc aatcatgcct ggagtggtag aaattgcaca
     4981 agaaatcttt ggagtaactg taaagctcca tgttccaaat caagtcggta ttagaaatcc
     5041 aatgttttca aacgttatca gtttggtaga atatgttggt atgatgtctg aagtagacgt
     5101 tttagcacaa actgcagttt caggagaaga acttttgcga cgcaagccta tcgatttcag
     5161 tggtcaagaa tcttatttac cagattatga tgattcaaga agaccagaat cgaccattgg
     5221 ctatgaacaa caagcgtcac aaacagcata tgattcacaa gttccgagtg atcctaaaca
     5281 aaaaatttca gaacgtgttc gtggcatatt tgggagtatg tttgattaaa agtaataaag
     5341 tgaggagata aaatggcatt ttcatttgat actgcatcaa ttcaaggtgc aattataaaa
     5401 gtaattggag tcggcggagg tggcggaaat gccattaatc gtatgattga tgaaggtgtt
     5461 gctggtgtcg agttcatcgc agcaaacaca gacattcagg cattaagctc atcaaaagct
     5521 gaaacggtta ttcaactagg ccctaaatta actcgtggac ttggtgctgg aggacaacct
     5581 gaagtaggac gtaaagctgc tgaagaaagc gaagaaattt taacagaagc tcttacagga
     5641 gcggacatgg tatttattac tgccggtatg ggtggtggct ctgggacagg ggctgcaccg
     5701 gttattgctc gtatcgctaa aagtttggga gccttgacag tagctgttgt tactcgcccg
     5761 tttggttttg aaggtaacaa acgtggtaat tttgctattg aaggtatcga agaactacgt
     5821 gaacaagttg atactttgtt aattatttca aataataacc ttcttgagat tgttgataaa
     5881 aagacacctt tattagaagc acttagtgaa gctgataatg ttttacgcca gggagttcaa
     5941 gggataaccg acttaattac tagtcctggc cttatcaatc tcgattttgc cgacgtgaaa
     6001 acagttatgg caaataaagg gaatgccttg atggggattg ggattggttc tggagaagag
     6061 cgcattgttg aggcggcgcg taaggcaatc tattcacccc tattagaacc gactattgat
     6121 ggtgcacaag acgttattgt gaacgttaca ggaggtctcg acatgacact cacagaagct
     6181 gaagaagcct ctgaaattgt tgggcaagct gctggtcaag gcgttaacat ttggttagga
     6241 acatctattg atgatactat gaaagatgac atccgtgtga ctgttgtagc aactggagtg
     6301 cgccaagaaa aagccgaaca agtttcaggt tttcgtcagc ctaggacttt tacccaaacc
     6361 aacgcgcagc aagtagcggg tgcacaatat gcatcagatc aagcaaaaca gtcggttcaa
     6421 ccagggtttg atcgtcgctc aaattttgac tttgacatgg gggagtctcg cgagatacca
     6481 agtgcacaaa aggtaatttc taatcataat caaaatcaag gttcagcttt tggaaattgg
     6541 gatttgagac gtgataatat ttctcgtcca acagaaggtg aattggataa ccatcttaat
     6601 atgtcaacgt tctcagctaa cgatgacagt gatgatgagt tagaaacgcc tccattcttt
     6661 aaaaaccgtt aataatggat ttactgacaa ataaaaagaa aatttttgag actatccgct
     6721 tatctacaga ggcagcaaat aggactactg atagtgtttc agttattgct gtaacaaaat
     6781 atgtggatag tacaattgca ggtcagctta tcgaagcagg aattgagcac attgccgaaa
     6841 accgtgttga taaatttctt gaaaagtatg atgcgttaaa gtatatgcca gtaaagtggc
     6901 atttaatcgg taccttacaa cgtcgtaaag tcaaggaagt tatcaattat gtcgattatt
     6961 ttcacgctct agattctgtg agattagctt tggaaatcaa caagagagct gaccatcctg
     7021 tgaagtgttt tctacaagtt aatatttcta aagaagagag taaacatggt tttaacattt
     7081 ctgagattga tgaagcgatt gaagaaatag gtaagatgga gaagatacag ttagttggtt
     7141 taatgactat ggcaccagca aatgccagta aagaaagtat tataactatt tttcgacaag
     7201 caaatcaatt aagaaaaaac ttgcagttaa aaaaaagaaa gaatatgcct tttacagaat
     7261 tgagcatggg catgagtaac gattatccaa ttgctattca agaaggctca acttttattc
     7321 ggattggtag agctttcttt cactaatgga gaataagatg gcttttaaag atacatttaa
     7381 caagatgatt tcttattttg acacggatga ggttaacgaa gttgaagaag atgttgcagc
     7441 atcaactgat aacgttattc caagatcaca acaatcagtc agagcaagta gtcatccaaa
     7501 acaagaacct agaaacaatc acgtacaaca agatcatcaa gcgagatccc aagaacagac
     7561 aaggtcacaa atgcatccaa aacatggtac ttctgaacgc tattatcagc agtctcagcc
     7621 aaaagaaggc catgaaatgg ttgacagaag aaaacggatg agcacttcta gtattgcaaa
     7681 tcgccgtgag cagtatcaac aatcaacttg ttcagatcaa acaactattg ccttaaagta
     7741 tcctcgtaaa tatgaggatg ctcaagaaat tgtggatctt ttaatagtta atgaatgcgt
     7801 tttgattgat tttcagttta tgctagatgc tcaggctaga cggtgtttag attttattga
     7861 tggtgctagt aaagtgctct atggtagctt acaaaaggtc ggctcttcaa tgtacttact
     7921 ggctccgtca aatgtatccg tcaatataga agaaatgact atcccacata ctacacaaga
     7981 tattggcttt gattttgata tgaaaaggcg gtaaataaat gatattaata ctatctattc
     8041 ttcttcgtct gatcaaagtt tacacttatt tattgattgc ttacgcatta atgtcatggt
     8101 ttcctggggc atatgattca aaaattgggc gtttgattag tggtatcgtt gaaccaattt
     8161 taaaaccttt tagagcattt aatttacaat ttgccggtct tgacttcact atttttgtcg
     8221 tcattattag tttgaatttt ttagctcaag ttttggtccg tgtgtttatt taatggttag
     8281 tcatagtaag atttatcagc attttcacca agaagaatat ccttttattg atagaatgtc
     8341 tgatatgatt aatagagttg aagattacta tcttttagaa gttactgagt ttttaaatcc
     8401 tagagaagtg atgattttaa aaagtttgat tgctttaaca gatctaaaaa tgttcgtatc
     8461 aacagattac tacccaagcg aatatggtcg tgtcattatt gcacctggtt actatgactt
     8521 agaacaaagt gattttcaaa tagctttagt agagataagt tatcaggcaa agtttaatca
     8581 gttgacacat agtcaaattt taggaacttt aattaatgaa ttaggagtaa agcgaaattt
     8641 atttggagat gtttttgttg aaatgggata tgcccagctc atgattaagc gggagttatt
     8701 ggattatttt ttaggaacaa ttactaaaat agctaaaact agtgtgaaat taagagaagt
     8761 taactttgat cagttaatta ggtctattga taacagccag accctggata ttctagtttc
     8821 tagttttcga ttagatggtg tagttgctac tatcttaaaa aaatctcgaa cgcaagttat
     8881 agcattaatt gaagcaaata agattaaggt aaactatcga gttgctaata aagcttcaga
     8941 taatctagtc ataggggata tggtgagtat cagaggtcac gggcgtttta ctcttttagc
     9001 agataatgga gtgaccaaac atggcaaaca aaaaataaca ctaagtaaaa tgatacataa
     9061 ataaggagat acaatggcac ttacaacgct agaaattaaa gataaaactt ttaagacaaa
     9121 attccgagga tactgtgaag aagaagtcaa tgaattcctt gatattgttg ttgatgatta
     9181 cgaagctctt gtacgtaaaa atcgtgataa cgaagcaaga attaaagatc ttgaagaaaa
     9241 attatcttac tttgatgaaa tgaaagagtc acttagccag tctgtaatct tagctcaaga
     9301 aacagctgaa aaagtgaaag caacggctaa tgcggaagca acaaacttgg ttagtaaagc
     9361 gacttatgat gctcagcatt tattagatga atctaaagtt aaagccaatc aaatgttgcg
     9421 tgatgcaact gatgaggcta agcgagttgc gattgaaaca gaagaactaa agcgtcaaac
     9481 acgtgtgttc caccaacgtt taatctcatc tattgagtca caattaagct tatcaaattc
     9541 acctgaatgg gatgaactgc tacaaccgac tgcaatctat cttcaaaatt ctgacgatgc
     9601 ttttaaggaa gtcgtgaaga ccgttcttaa tgaagacatt cctgaatctg atgatagtgc
     9661 ctcttttgat gcaacacgtc agtttactcc agaagagtta gaagaattgc aacgtcgtgt
     9721 tgatgaaagc aataaggagt tagaggctta ccaacttgac tctcaatctg attctacgac
     9781 tgagccagag gtaaatctca gtgaaacaca aacgtttaaa ttaaatatct aaataaagaa
     9841 aaacaagatg atttgcttat aatcacagca agcgggagat gatgagagtc tcgtattccc
     9901 taagtattat cattatcatt ttctaaaatt ttcgctgaac tacagtaagc gaggaggttt
     9961 gttcacctta cgagcaaaga gggagcggta agtcttatga tatattatcg ctctgaacta
    10021 aggtggtacc acgagctttc gtcctttttg agacgaggct tttttcgtta tagtcgcaag
    10081 tttgtttaaa aggagtttga aataatgaaa ttaaaagaga cacttaatct aggaaaaaca
    10141 gcctttccaa tgcgcgcagg tcttcctaat aaagaaccac aatggcaagc agcttgggaa
    10201 caagcagagc tttacaaaaa acgtcaagaa ttgaatgcag gtaagcctgc ctttcatctt
    10261 catgatggac ctccatacgc taacggcaat attcacgttg ggcatgccct taataaaatt
    10321 tcaaaagata tcattgtgcg ttctaagtcc atgtctggct ttcaagcgcc ttatgtacct
    10381 ggttgggaca cacacggcct tccaatcgaa caagtcttgg ctaagcaagg aatcaaacgt
    10441 aaggagatgg atttagcaga ataccttgag atgtgtcgtc aatacgcttt aagtcaggtt
    10501 gataaacagc gagatgattt caaacggtta ggtgtttcgg cagactggga aaatccttat
    10561 gtcactttgg acccacagtt tgaagctgat caaattcgtg tttttggagc gatggctgaa
    10621 aaaggttaca tctatcgtgg tgctaaacct gtttattggt catggtcttc agaatcggcc
    10681 ttggcagaag ctgaaattga ataccatgac atcgattcaa cctctttata ctacgccaat
    10741 aaagttaaag atggtaaagg catccttgat actaatacgt atatcgttgt ttggaccaca
    10801 acaccattta ccgttacagc atcacgcggt ttgactgttg gtcctgatat ggattatctt
    10861 gtggtgaaac ctgctggttc agaccgtcaa tatgttgtgg cagaaggtct tttggatagc
    10921 cttgctggaa aatttggctg ggaatctttt gaaactttag ccagccataa aggagctgat
    10981 ttagagtaca ttgtgacaga acacccatgg gatactgacg tagaagaatt ggttatcctt
    11041 ggtgaccatg ttacccttga gtcagggaca gggattgtcc acacggcgcc aggttttggt
    11101 gaagatgact acaatgttgg gacaaaatat aaactggaag ttgctgtgac tgttgatgaa
    11161 cgtggcctaa tgatggaaaa tgcaggtcca gatttccatg gacaatttta taacaaggta
    11221 acgccgattg ccattgataa acttggtgat cttttattgg cacaagaagt gatcaatcac
    11281 tcttatccat ttgactggcg gacgaaaaaa ccaatcatct ggcgtgctgt accgcaatgg
    11341 tttgcttctg tttctgattt ccgtcaagat attttggatg aaattgaaaa aacaaccttc
    11401 catccatcat ggggtgaaac acgcctttac aatatgattc gtgatcgtgg cgactgggtt
    11461 atttctcgtc aacgtgcttg gggagttccg cttcctatct tctatgccga agatggcaca
    11521 gctattatga ctaaagaagt gacagatcat gtggctgatt tgttccaaga aaatggatca
    11581 atcatttggt ggcaaaaaga agctaaagac ctattaccag agggcttcac tcatccaggg
    11641 tcaccaaatg gcgagtttac aaaagaaact gatatcatgg acgtttggtt tgactctggt
    11701 tcttcttgga atggagtcat gaatgctaga gaaaatcttt cctacccagc ggatctctac
    11761 ttagaaggct ctgaccagta tcgtggttgg ttcaattcat ccttaattac atctgtggct
    11821 gttaatgggc atgctccata taaggctatt ttatcgcaag gctttgttct tgatggtaag
    11881 ggcgaaaaaa tgtctaaatc aaaaggcaat atcatttctc caaatgatgt ggctaaacaa
    11941 tatggtgcag atattcttcg actttgggta gcttctgtgg ataccgacaa tgatgtccgt
    12001 gtttctatgg agatccttgg tcaagtctct gaaacctacc gtaaaatccg taacaccctt
    12061 cgtttcttga ttgctaatac atctgatttc aaccctgcta cggatacagt agcttatgca
    12121 gacttaggag ctgttgataa gtacatgaca attgtcttta atcagttggt agcaacgatt
    12181 actgacgctt atgagcgtta tgactttatg gctatttaca aagcagtggt gaactttgtt
    12241 acggttgatt tatcagcctt ctatcttgat tttgctaaag acgttgtcta tattgaagct
    12301 gccaatagct tagaacgtcg tcgcatgcag acagtcttct atgatatctt ggttaagatt
    12361 accaagttat tgacgccaat cttgcctcac actactgaag aaatctggtc ttatctagag
    12421 catgagtcag aagcatttgt tcaattgaca gaaatgcctg tggcagaaac cttctcagct
    12481 caagaggata ttttagaagc ttggtcagct ttcatgactt tgcgtaccca agctcaaaaa
    12541 gctttggaag aagcgcgaaa tgccaaggtt attgggaaat cattggaagc tcatttgact
    12601 atctacgcta gcgaagaggt gaaaacctta ttgactgctt tagacagcga tattgctttg
    12661 cttttgatcg tgtctcaatt aaccattgct gacttggcag atgcgcctgc ggatgcagtg
    12721 gcatttgaag gtattgcctt tatggtagaa catgccatag gtgaagtttg tgagcgttca
    12781 cgccgcatcg acccaactac tcgcatgcgt tcttacaatg catttgtctg tgatcacagc
    12841 gctaaaatca ttgaagaaaa tttcccagaa gctgtggctg aagggtttga agagagtggc
    12901 aaataagtct tatcaagact tgaagtgaca ctgagtaaga agacaaccat cgggtcttag
    12961 gacctgatgg ttttttagta gagctgatta cgttcattag ccagctttaa tgagagacaa
    13021 agtagaagaa atttagacta ctttttttat ttcttttaag aaaattgctg gcttcatata
    13081 aggactatta agatagagtt cacctttcgc taataaaaaa acttagctct tttagaaggg
    13141 ctaagttttg tctgaatagc tggctactgt ttatacagaa gtttgaaatg aaatttcaat
    13201 aaggttattc gtgtgaagtg tttttgattg tccaggaata acaattttag gatcaatttt
    13261 acgttttaaa aaaaattcgc gaatgatatc aagttcatcc tctttgatgg cacggtattt
    13321 atttgaaata agtagttcgt aatgttttgg tgcttgggta aaaatgacat ctgtattccc
    13381 tgctgagtat acttcgacta aataggcatc ggtgtttttt agttgatttc tgatgagttc
    13441 tgggtgactg ctagtggtat tgatgagtct cataaatgtc ctccttagta ttcctgtacc
    13501 tattatagca ctaatttaaa gccttagtta gtagaatgat gctttttttg ccattccttg
    13561 actccccatt gatggctctc tcgttttaat ttagcaattg cctcgatagg agaaaaccaa
    13621 cctaagttat taaaatcttc taagggctta gaaacggctt gaaatgcagt tacttcataa
    13681 agataggcag gatggtagaa gtgggtatca cgatggcgag agtagaaata ttcatcagcc
    13741 tgaccgtaat atgaaccaat agtagcagaa aaaccaagtt cttcaatcag ttcacgctct
    13801 agggcctgaa gctgaccttc acctgcttca atttcaccac caggtaaaaa ccaggaacca
    13861 ttaggagctt gaacaagaat aatttttgtt tgttcgtgat tgggaatgat agcatagaca
    13921 ccgtaacgtg tgacatagtc tttatgtgca tttttatgtc caaatgttgg aatcatcatt
    13981 ttatttacct ttcctttttc ttcatta
//
LOCUS       DAUCWU010000040        13607 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUCWU010000040 DAUCWU010000000
VERSION     DAUCWU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.040"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            269..1171
                     /locus_tag="VGY24_001082"
     CDS             269..1171
                     /locus_tag="VGY24_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ4284065.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            1171..1986
                     /locus_tag="VGY24_001083"
     CDS             1171..1986
                     /locus_tag="VGY24_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ4284066.1"
                     /translation="MIRFLILSGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(2085..3509)
                     /locus_tag="VGY24_001084"
     CDS             complement(2085..3509)
                     /locus_tag="VGY24_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ4284067.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFGELLNYGIDPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(3528..5390)
                     /locus_tag="VGY24_001085"
     CDS             complement(3528..5390)
                     /locus_tag="VGY24_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ4284068.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKAKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLAYNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFINALIAGTATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDFVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKIVTKEDALLVIL"
     gene            complement(5392..6234)
                     /locus_tag="VGY24_001086"
     CDS             complement(5392..6234)
                     /locus_tag="VGY24_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ4284069.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            6562..7929
                     /locus_tag="VGY24_001087"
     CDS             6562..7929
                     /locus_tag="VGY24_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4284070.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNMRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(7983..9533)
                     /gene="ftsY"
                     /locus_tag="VGY24_001088"
     CDS             complement(7983..9533)
                     /gene="ftsY"
                     /locus_tag="VGY24_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ4284071.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEVVQQEDVSSEGAGSVENGPEAALVNAQVEEETGDNSNHSSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHVTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEVLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(9533..10357)
                     /locus_tag="VGY24_001089"
     CDS             complement(9533..10357)
                     /locus_tag="VGY24_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4284072.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(10361..11158)
                     /locus_tag="VGY24_001090"
     CDS             complement(10361..11158)
                     /locus_tag="VGY24_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4284073.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            11462..12588
                     /locus_tag="VGY24_001091"
     CDS             join(11462..11696,11696..12588)
                     /locus_tag="VGY24_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4284074.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
     gene            complement(12959..13375)
                     /locus_tag="VGY24_001092"
     CDS             complement(12959..13375)
                     /locus_tag="VGY24_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284075.1"
                     /translation="MNYKIFIRTKSGDIFDIADGGRIDDMVSKFNKVNVLGVCMGIGT
                     TVLSQEIEYEIEDRIMILVEKIDVKIYKNCLELANKLSGYHCSVFEFPYKKIKKFFKH
                     ELYSRHNYIIVRLDGSMEYRFSSVALKNKIKSILDS"
BASE COUNT         3995 a         2954 c         2366 g         4292 t
ORIGIN      
        1 aaaaatgcta gcaggctttg catcaatccc tagttttgat aagcctttgg aagtcaatac
       61 tgatggtaaa ctagcgacca aagaaaaaac gacagaacaa acaaaaccaa gcaaaccata
      121 gctagtttgt ctcattctct taacatatcg tccattcgtt taaactgagt tgagagattt
      181 ttagcgatca ggctaacaaa acgtatcagt cgaggctgaa cattcgtgtt tcagcctcct
      241 cttttatcat ctagaaagga tatggtgaat gtcactcttt tccaaccttc ctcccagtgt
      301 cttgcaatgg tttgcgattt ttatgtccat tatcattgaa gctcttcctt ttgtgctcct
      361 aggaactatt ttatctggtt gtattgaagt ctttgtaacg ccagaattgg ttcaaaaata
      421 cctacctaag caaaaatgct tgcgcattct gtttggaacc tttgttggat ttgtttttcc
      481 ttcttgtgaa tgcgggatta ttcccatcat caaccgcttt ttggaaaaaa aggtgcctag
      541 ttatacggcc gtcccttttc tggcaacagc gcctattatt aatcctattg ttttgttcgc
      601 tacttattct gcttttggga attctttgcg ctttctgata cttcgcttgg tcggtgctgc
      661 actagtagcc attacacttg gggtaatgtt agcttttatt gtagatgata acattctcaa
      721 ggataatgct caacccgtgc attttcatga ttattctcat gaaagcttgc caaaacgcat
      781 ttatctagca ctggtacatg ctattgatga attcttcgac accggtaggt atctcgtatt
      841 tggaaccttg attgcctctg ccatgcaaat atatgtccca actcgtgtgt tgacaacgat
      901 tgggcacaat cccttgacag ccattttgat catgatgctg atggctttca ttctatcatt
      961 atgtagtgaa gccgatgcct ttatcggagc ttccttatta tccacctttg gagtggctcc
     1021 agttcttgcc tttcttttaa ttggtcctat ggtggatatt aaaaacctta tgatgatggt
     1081 aaaggctttt aaaggacgct tcattgtcca atttatcggc gtatccgttc ttatgattgc
     1141 cgtttattgt ttacttgtgg gggtgctcta atgattcgct ttttaatttt atctggctat
     1201 tttgaactaa ccatgtatct ccagctgtct ggcaaactgg atcagtacat caatgttcgg
     1261 tattcttacc ttgcttatat ttccatggtt ttatctttca ttttagctct tgttcaactc
     1321 tatacctgga tgaaaaacat taaggtacat agccatttaa caggaaaaat cgcaaggctg
     1381 accagtcctt ttatcttggt ctttcctgtt ttgattggtc tccttgtgcc gaccgtgacc
     1441 ttagattcca ccacggtttc tgctaaaggc tatactttcc cattggctgc gggagcctct
     1501 aaaacgggag tcagtgatga cggtaccacc attcaatacc tcaaacctga taccagcctt
     1561 tactttacaa agtctgccta ccaaaaggag atgcgtcaag aactccataa atataaaggc
     1621 aagaaaccgg tcaccattac cactgaaaat tatatggaag tgatggaatt gatctatctc
     1681 tacccagatg agtttcttga tcgtgacatc caatacactg gctttgttta taacgaaccg
     1741 ggccatgaca actaccaatt ccttttccgc ttcggcatta ttcactgtat tgccgactca
     1801 ggagtgtacg gacttttgac cacaggtaat caaaccagct accctaacaa cacatggcta
     1861 acagttaaag gtcgccttca tatggaatat gataaaaact tagaacaaca tctacctgtc
     1921 cttcaacttg ctgaggttca tcaaaccaaa gaacctaata atccttatgt ttaccgcgtt
     1981 ttttaaagac taataaaaaa gcacttgttt tttatcaaaa caaatgcttt ttgagtttta
     2041 tcagttagca taggaacaga gtcaccatct taaacaagac ctatttagga aaaattatcc
     2101 tcaaggtaac gtccattggt ttgaatgact tgtcggtacc aatcaaatga ttttttctta
     2161 tagcgtgtga gttgaccagt accatcatca tttcggtcaa cataaataaa accgtagcgc
     2221 ttacttaatt gggctgttga catagagaca caatcaatac agccccaagc agtgtaaccc
     2281 atgacctcaa cgccatcttc aatcgctttg gcaacttgta cgagatgctg actcatgtag
     2341 tcaatgcgat aatcatcgtg aaccgtcatg ctgccatctg cggtttgcac cagctgatcc
     2401 tttgcaccta agccattttc aacaataaac aaaggaatct ggtagcggtc atagtactga
     2461 ttcaagacca atcgcagacc aattgggtct atctgccaac cccactccga agattctaaa
     2521 tatggattgg acaaaccacc tagaatattt ccctgaccag atgtgtaagc ttcaggattg
     2581 tgggcttgtg tgacactcat gtaatagcta aaggatagaa aatcaaccgt atgttccgct
     2641 aataattcct tgtctccgtc ttcaaaaaca atctcaatcc cattttcttt aaaatacgac
     2701 ttaatatatg aaggatactt gcctctcaca tgaatatctg aaaataagag attaagattt
     2761 tcaaatgcat gcgcagcaag tatatcacga ggatcagagg tcattgggta agcaggcatc
     2821 gccaaaatca tacacccaac tttaacatca ggattgattt catgcgctag ctttgtgaca
     2881 ctagcagatg ctaccaattc atgatgaata gcttggtata aatcttgcag gctcagcttt
     2941 tctttttctg tcatgatgcc accactggta aagggcatgt gtaaaatcga gttgacttcg
     3001 ttgaaggtca accagtattt gactttgtcc ttatagcgtt ccatgactgt ctgagcaaag
     3061 cgctcaaaaa agccaatcag acgtctatct gtccaaccat tataggcttt ggccaaatgc
     3121 agaggtgtct cataatgcga caaggtcact aaggggtcga taccatagtt caacaattca
     3181 ccgaataagt catcataaaa ttgcaaacct gcttcattgg gctggtcatc gtcaccattt
     3241 ggaaaaatac gagaccaagc aatagacgtt cgaaagactt taaagcccat ctcggcaaaa
     3301 agagcaatgt cttctttgta gcggtggtaa aaatcaatcg cctctaaagt taaattatcg
     3361 ctggtaggac tatctgtcca ttctcctagt ccaccatttg gtagaacatc ttgtacggac
     3421 aaaccttttg cgtctgcctc aaatgctcct tccacttggt tggcagctac tgcgccgccc
     3481 cataagaaat cttttggaaa aattcccatt ctccctctcc ttttaggtta taaaatgact
     3541 aacaaagcgt cctctttagt cactattttt ttgtcatctt gaataataat ctccaactgc
     3601 tcagccgaat tggtcacgac cacaggactg atgagtgaat agccttttga agtaatgaaa
     3661 tcaatatcca tttttagaag tgcctggcct cgtttcacga aatcgccaac agacactaac
     3721 tgctcgaaac cttgcccttt taactcaacc gtatcaatac cgacatgaat caagacttcc
     3781 acaccacgtg tgttttttaa ggcaacagca tgcttggttg gaaagaaagt gatgacttca
     3841 ccatcaaagg gggcatagac cacaccttcg cttggtaata tggcaaatcc ttctcctaat
     3901 aacttggaag aaaaggtttc atcaggaact tctgatagaa aaagcatctc acctgtcatt
     3961 ggagcataca acgtttgttt ggttgacaag ccgctcttaa cttgtgacat atctgctgca
     4021 acactcactt gctttgggga ctcttcatca attcccatga accaagtcaa actgaaggct
     4081 aacacaatcg tcgcagttcc ggcaatcaaa gcatttataa aattagctcc gcctgatggg
     4141 ttgatgtact gtggtaaacc aataatagaa ggtacgacaa aggtataaga agcaatattg
     4201 acaaaaccaa taaaggctcc aaccagacca cctgaaatca tggctgcata gagtggcttt
     4261 ttaaatttca gtgtgacccc ataaagtgcc ggttccgtga tacctgccag caaagctgaa
     4321 atccccgcag caagggcaac ttgacgtgtt tgtttctgct ttgttttaaa ggcaacagcc
     4381 aaagatgcgg ctccttgagc caaattataa gccaacattg ctggtaaaat caagacatct
     4441 ggtgttgcga ccgaagcggc caaaaagatt ggtgcaaaag cccagtgcat ccccgtcatg
     4501 acaacaagcg gcataatcgc tcccaaaatg gcaagtgcta gccacggagc cacatgataa
     4561 atagcgagca tggtattaga taacccttga ccaataatca caccaagagg acctacaacc
     4621 actaaagcca aaaagccaga aatcaaaatg attaaggttg gctgtaagaa gcttttcatc
     4681 acgcttggca ccaagcgatt gacccatttt tcaatgtatt gcatcaagta aaccatcatc
     4741 aaaatgggaa taacagatga gccatagcta gcaggagtga ctggtgctcc aaataaagtc
     4801 aatggtttac cttctgctac cattgcaaca aaggcaggat gcaataaaat ccctgcgatc
     4861 gtcgctgcta aaacaggtgt caccttaaag cgcgccgctg ctgaataggc aatcatgaca
     4921 ggcataaaat aataagccgc atcgccaaag aagttaataa aggcaacggt ttgagagtcc
     4981 gcactcgcta agccaagcat aggaagcaag atccccacca ctttgagcat tccaccacca
     5041 agaagtgctg gaatcagcgg tgtaatcacg ccagcaatgg tttcaatcaa gcgctcaagg
     5101 atacttcctt tcgcttttga agaatgatct tcatcttgat tgtcaaaatg ccctaggctt
     5161 aaaaaggctt ggtaataatt attgacatca ttgcccaaga taatttggta ctggccgttt
     5221 ttgcgcatca cgccaatcac atttgaaatg gctttgactt gctgatcttt agctttctca
     5281 tcatttttca gtactaagcg tagacgtgtt acacagtgtg tcacacgttg aatatttgtt
     5341 ttgccaccaa ccgccgctaa aatagccttg gcagtttctt gatacgtcat attaagtctc
     5401 ctttaaatgt ttcactaaac gtttaacatg aaccgtcaaa tacaaaagtt cgcttgaatt
     5461 aaggtggtag ttgtaccgct tttttatctc ctcactaata tctaagacgc accggtattc
     5521 tttagggtat ttcgttttca ccactagaag cagttccgca tcatcatcat cgtaagacat
     5581 ctctgataac acacgttgag caaataattt aatatgggtc ataaagcggt aataatcgat
     5641 ggatgtgtca tccaattctg ttttgaagtc tgttttgatt ttttgctcaa tataagaaac
     5701 aatctccgtc aggcgatgtg gctccttaaa aggagtgtcc aaactagcat tgacaaaatg
     5761 catggcaata aaagctgctt catcaatagc tagagtgact cctagattac gctcaattag
     5821 ctctaaggct tttaccccta gactatattc atcaggataa tagcgttgaa tttcccaccg
     5881 tagtgggtta tgaatcaaca ttccttgctc atgtctttca atagcagagt gaatatgatc
     5941 cgttaaatta atatagagaa tttcatctaa ggttttgcct aatttaattt tacctagatt
     6001 gataattctc tctgagcaag caacaacttc ttgtggcacc gtaataaata actctgtgaa
     6061 gcgattcata ttgtcactat tttttaaaac aaatgatgtt tcgatagcat taagctcaat
     6121 actatctcca actttttttc caaaggcaat cccttttccc atcaataaaa tatctaatcc
     6181 ttgatggttg gtcgaaatcg ccgcattatg gtttaaaacg cgtttaatca acataaaagt
     6241 cctagtcctt tagtatcaca ataaaaaatg acctatccaa aagctcgcta aaaagcactt
     6301 ttcgaatagg tccctgctca caaaactgtg ataacatcct ttaatattct gattgttatg
     6361 tctctagcct gcttacctga agtaatcaca tctgagaacg ctttaatgtt atcatctctt
     6421 taagattctg tcaagcgttt tcaaaaaatt ctctgaaaga gcaactattg ttattcgtat
     6481 ctattgattt agcaaaaaag agtttctgac aattttaaga ttatatgata caataacaat
     6541 cgattagata aaggagttat catgtcacat catcaacaaa ccgtttcaaa acaaacaatt
     6601 atggcgatta tcgccatagc actcattggt ttttcaggaa ttttgtctga aaccagtatg
     6661 aatgtcacct tcccgacact gatgtcagtc tatcagttgc ccttaaatag cttgcaatgg
     6721 atgacgacca tttatttact agcagtggcg attatgatga ccacttcggc tacactgaaa
     6781 aaaaatatgc gggaaagacc cctctttttt atggccacag gtctttttac atttggcacc
     6841 atcttggccg ttctgaccca gtcctttgcg atcatgttgc tagcccgcat ttttcaaggc
     6901 attggtactg gtctggtaat gcctcagatg tttaatatta ttttagagcg tgtcccaatg
     6961 cataaggtag gtctatttat gggatttgct ggtcttatta ttagcttagc acctgctttt
     7021 gggcctactt atgggggctt tatgattagc cattttagtt ggcaatggat ttttatctgt
     7081 atactccctg taccactgat tgcaggtatt ctagcttatt attacctcga agattctcca
     7141 gtaagcgaaa aagtaccctt tgactggttg gcatttattg cactatcgat tagcttaact
     7201 tctgccttat tagctattac tagtctagaa aacggaagtg ttaatttgta ttacttaggg
     7261 ctttttattc tcagctttgt cctcttcctc tacaagaatc ttacagctaa gcaacccttt
     7321 cttgatattc gcattctcaa aattccctct ctaacctttg gcctgattcc cttttttgtc
     7381 ttccagctga ttaatttagg cataaatttt ctaacgccaa actttattgt catggaaaag
     7441 attgctaata gttctcaagc tggtatggta ttactacctg gtaccttact cggagctcta
     7501 ctagcacctg cttttggtaa actttatgat caaaaaggag caagactttc gctttattta
     7561 ggaaatgcct tatttagttt atcattgatt attatgacac ttcaaacaag acattttatg
     7621 cttttaccat tcactctttt atatatttta ttcacgtttg ggcgtaacat gggctttaat
     7681 aatagcttag ccacggctat tcgagaattg cctgccgaga aaaatgccga tgccacggcc
     7741 atttttcaga tgatgcagca atttgctggc gctctaggaa ctgctatggc atcactgata
     7801 gcaaatagtc aaccagaatt cacaagcggt gtccagtctg tctaccttct ctttactatt
     7861 tttgctctac ttgattttat ctttttcttt gctatgtttt accatttagg gaaaaaagga
     7921 cttgcctaag ctgacttact taaaaaccag cctcgttctg ttgaacaaga ctggtttttt
     7981 tcttataaaa taccttctaa aagacctttc ataaagtctt ctgagtggaa ttctccaata
     8041 tcatccactt tttcaccaaa tccgataaac ttgactggaa tgtctaattc ttgacggatg
     8101 gctaggacta caccaccttt ggccgtgcca tcaattttcg ttaaaatcaa acctgttaat
     8161 ggtgttattt ttgaaaattc cttggcttgg ctaagcgcat tttgacctgt tgacgcatct
     8221 aaggccaaaa gcgtttcatg aggggcatct ggtagcacgc gcttaatgat acgacccatt
     8281 ttttccagct ctgccatgag attttctttg ttttgcaggc gaccagccgt gtcaatcagg
     8341 agaatatcta caccttttgc cacagctttt tccatcccgt caaaaacaac agacgctgga
     8401 tcagcctttt cagggccagt aatgactggc acatctactc ggcgtcccca ttcgactagc
     8461 tgagccacag cccctgctcg gaaggtatct gctgccacca acatgacttt tttaccttct
     8521 tgtttgtaac ggtaagccag cttcccgatt gaagtcgttt taccaacccc gttaacacct
     8581 acaaaaagca taaccgtcaa gccatcttga taattaatgg cttcattata acgcccgtct
     8641 ttttcataga tatctaccaa tttttccaca atcacacgtt tcaaggcatc tggttttttc
     8701 gcattttcca gtttggcttc ataacgcaac tcctctgtca aagttgtcgc cacatgtaca
     8761 ccgacatctg ataagatcag cacttcttcc aagtcttcaa agaattcttc atccactcgc
     8821 ctgaaattgg caaagaaact gttcagacga gctgagaagc cagtccgcgt ttttttcaag
     8881 ctacgcttgt atttttcttg atctgtctca ggaatggtaa cgtgggttgc cttgttttga
     8941 gaagcttctg ctgaggtaga ggcttgattt tcttgtggaa cttcttctgg catcactgga
     9001 accgttgccg ctgctttttc ttgaaggctt ttttcaagag cagccttacg acggtagtag
     9061 tcttccataa cctgttggct actggattgc tcttttgaag tgtctactgc ttcttcattc
     9121 gccgaatcat ttgaggcatc agcttgagct tgttttgttg gaaactgttc cacaactggc
     9181 tggtcaacaa ggtcttttgg ttctgatgcc gtagattgac taagttcact taccttaagg
     9241 tctgttttgt cggcagcaaa ctcattggta tcttctgatg aatgattact attatcacct
     9301 gtttcttcct caacctgtgc atttactaaa gctgcttcgg gaccattttc caccgaacct
     9361 gcgccctctg aagaaacgtc ctcttgttga accacctcaa tcttgtcttg ctcattggct
     9421 ttttcagaaa gctcctcttt ctgagttgtt tctgtcaaaa gattttcttc taacttttcc
     9481 tcagctacct tcggggtttc ttttttccca aataaacgat caaataatcc cattaactct
     9541 cctcttctaa aataaatgtc ttcacagctt ctgcgactcc agactcatca ttggtcaagg
     9601 tggttacggc atcggcaacg gctttagctc ctgaaacacc attagccata gctactccaa
     9661 gtcctgccca ttctaacatc gcaaagtcgt tagcttcgtc tcccatagcc atgacatgtc
     9721 tagcgtcaag gccaagatgc tgacaaagca agtttaatcc aaaagcttta tcaatgcctt
     9781 ttggcatgat ttcaaaaata atatctcgtg atttaaaggc ttcaaaatcc tcaaataagc
     9841 ttggggatag ctttataatc tgctgatcca aaaagtctgg atcggtgact gtcacaattt
     9901 tgttataggt aatgtctttt ggtagttccg ctacagatgt cacctcaata aaggtcaaca
     9961 gtgggttagc tagatggtac tgcgaatgcc taccgtcctt acttggaatg ctatacacat
    10021 ccccaccact gataatatct gttggtaagc caaccgcctc taaagcttgc tgaatctggc
    10081 aaacctggtc aaaactgaga gaactcttat ccaaaacctc ccctgtattt cgctgaacca
    10141 accccccatt aaaggtaata ctgtagtcat cgtggtcaag caagtccaac tcttctagca
    10201 agttaccgat ggcctttaga gggcgacctg tggtaataac caccttaacc cctttttctc
    10261 tagccgctgc taaggctttt ttattggcat ctgtgacaat tttttcagtg ttgtagaggg
    10321 taccgtctaa atcaagcgcc aaaattctaa tattcatgag ttacactagt ccttttaaat
    10381 acgttagtac tgctccgtca ttacagtggc caatgacttg gtctgaaatc actttgattt
    10441 caggtcttgc attttccgta gcaatagccc taccagcaaa ctccaacatc tggtaatcat
    10501 taaaattatc cccaaaagca atcgtttctg ctttcttaat gcccaaggct tgacaaagat
    10561 gctccatacc aaagccctta ttcacctctt ttaagataat atcaatggaa tcaaagccgg
    10621 tggtcactgc agtggcataa gggagggctt gattcaacca atcgcttcct tctaagacgg
    10681 tatggcctgt gaagttggta gaaactttaa aaatggcgtc attctccata tcttcaaaac
    10741 cattaataac cttaacatta gcatagtaat gttttgtttt ttgaatgtac tcttctgatg
    10801 cgccttttaa aatataggct gccttttgcc cagaaaaaac catacctgtt tctacatagt
    10861 gaggattggc taaaaccttt tttgcaactt cggtatactg ctctttggtc atcatatctg
    10921 caaatagaat ctcaccatgg tattgaacca cggaaccatt ttctgcaata acagcaatct
    10981 gatccaaaaa gggctcaaag agctgatcaa tggctaagag cgagcggcca ctcgaaaccg
    11041 caaataaaat gcctttttcg gctaactttg gcaagagcgc agctagttgc tcttgattat
    11101 aagtaccatc ttcagctagg aaggtaccat ccatatctgt tgctattagt tttatcatac
    11161 aatgtctcct ttttttactc taggggggtg caaacgccat ttagggtgtg cactttttaa
    11221 cgatttaaat aagccccatc atttctgcat tctgttcaat ttttcttatt atatcataaa
    11281 aagcagcttt cattccattc gtaaaaaaca gccagattat atttcgataa cctgaccgtt
    11341 tttgaagtga actgctactt ttttattttt ataaatgacc actggatgta tactataaaa
    11401 gtagacaaaa ctttgtgtgt tacaatctgt tttaaaagac acaaaaacga aaggacaacc
    11461 tatgtcaaca gttaaacgct acgatgaaga attcaaacaa tctcttgtca acctttatca
    11521 aactggaaaa actcagtctg aactctgtaa agactatggg gtatccgctt ctgcgcttgc
    11581 aaaatggatc aaacagtatt ctcaagtcaa actcgaagat aattctgtac ttactgccaa
    11641 gcaaatacaa gagctacaaa aacgtaatgc gcagcttgaa gaggagaacc ttatcctaaa
    11701 aaaagcaagt gccatattca tgcaaaactt aaagtaagac tcctcgctgt ctatcggtta
    11761 cgctttgaac acgccacaac aaccttgtgt cgtgttttac gtgtcaatcg ctccacttac
    11821 tataaatttc taaaacataa gccctccaaa agagaattgg ataatcaaat ttataaaaaa
    11881 caaatacttg agatttatac caaagcgaac aaaagacttg gtgtgaagtc tatcaaagtc
    11941 attcttcaaa gagactacga cacaaaaatc tctgaaggaa gaatttaccg tctgatgaag
    12001 aatatggcgc ttcctaaaat ggctaccgtt aagccaaaaa cagctcttaa aaggactcaa
    12061 aaaacgtatc ctcaaaactt actcaaccag aaatttaatc ctgacaaacc aaatcaagta
    12121 tggtctactg acttcaccta tatttctatt ggatataaga aatatgtcta tctctgcgca
    12181 atacttgatc tctattctag aaaatgtatt gcttggaaac tgagtcatca tatggatgca
    12241 aagttagcat gtgacactct agaattagct cttaataaaa gaaagattga aggaacactt
    12301 ctctttcatt ccgaccaagg gtcacaattt aaggccaggg aattgagaaa aataattgat
    12361 gacaacacta tcatgcattc tttttctaaa cctggatatc cttatgataa tgccgtaacg
    12421 gaagcatttt tcaagtattt aaagcataga caaatcaacc gaaaaaagta tcaaaatatc
    12481 aaacaggttc aattagactg ctttgaatac attgagaatt tttataacaa ttacaaccca
    12541 catacggcta atctaggact aacccctaat cagaaagaag aaaattagtt taacgtaata
    12601 aaataacacg gttttctgtc tacttatttg acattagtcc aaaatacatc ctaatggtgt
    12661 cgcttttttt atttcactag acattcgttc tagtttagga tataagaagg tctcaaattt
    12721 ttttcaggat gcttattcca aacatccaat cacctttcta tataactgtt catttagtgg
    12781 tatagacaaa cagaaatact ccgtttctag tttcatagaa gacggagtat tttttagtag
    12841 agacgtctgg attgatagat agttggtata tagctcatcc ctactaggct ttcattatag
    12901 actatggtgg gtttcgttga caacaatatt tgattattat tctaaatgac agtatacatc
    12961 aactatcaag tatgctttta attttatttt ttaaagctac agatgaaaat ctgtattcca
    13021 tacttccatc taatcgaaca ataatataat tatgtctcga atataattca tgtttaaaaa
    13081 actttttgat ttttttataa ggaaactcaa aaacggaaca atgataccca cttaatttat
    13141 ttgctaattc aagacaattc ttatagattt tcacatctat tttttcaact aatatcatta
    13201 ttctatcttc aatttcatac tcaatctctt gtgataaaac agtggttcct attcccatgc
    13261 aaactcctaa tacgtttact ttattaaatt tagaaaccat atcatcaatt ctaccaccat
    13321 cagcaatatc aaatatgtca cctgacttag ttcgtatgaa gattttatag ttcatacttg
    13381 aataatgtcc tgttccatat aaattagata atgaaaagta aatgcaatct actggtaaaa
    13441 agttttttac atactcatac aagtctaaaa cttttataaa ttcctgtttc accagtttgt
    13501 tacttatttt tgaaataaga ctaattaaga gttccatatt tattttgtct ttttcataaa
    13561 gacattttat gatttctatg tcattattac acaaatattt acccaat
//
LOCUS       DAUCWU010000041        13431 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUCWU010000041 DAUCWU010000000
VERSION     DAUCWU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.041"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            480..890
                     /locus_tag="VGY24_001093"
     CDS             480..890
                     /locus_tag="VGY24_001093"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ4284076.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(972..2984)
                     /locus_tag="VGY24_001094"
     CDS             complement(972..2984)
                     /locus_tag="VGY24_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ4284077.1"
                     /translation="MKKYFILKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGAL
                     DNTGTAYTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANS
                     ALLQDEPTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEH
                     EASHQTIVIANVIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQ
                     NYEHYQFLDVAETIAKYAKELQEQHVHAIVVLAHVPATSKDGVVDHEMATVMEKVNQI
                     YPEHSIDIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFMTTPSAN
                     VVTVAPGIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNL
                     VTTAQLTIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQM
                     TGQHIYDVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLT
                     TTYTVVVNDFLYGGGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGV
                     KNYVTSNLESSTKVNSAGKHSIISKVFRNRDDNIVSSEVISDLLTSTENTNNSLGKKE
                     TTTNKNTISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            3204..3854
                     /locus_tag="VGY24_001095"
     CDS             3204..3854
                     /locus_tag="VGY24_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ4284078.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            3857..4525
                     /locus_tag="VGY24_001096"
     CDS             3857..4525
                     /locus_tag="VGY24_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4284079.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            4534..5766
                     /locus_tag="VGY24_001097"
     CDS             4534..5766
                     /locus_tag="VGY24_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4284080.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSVIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVMI"
     gene            6060..6938
                     /gene="mvk"
                     /locus_tag="VGY24_001098"
     CDS             6060..6938
                     /gene="mvk"
                     /locus_tag="VGY24_001098"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ4284081.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            6920..7864
                     /gene="mvaD"
                     /locus_tag="VGY24_001099"
     CDS             6920..7864
                     /gene="mvaD"
                     /locus_tag="VGY24_001099"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ4284082.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFNTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDQWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYRAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            7857..8870
                     /locus_tag="VGY24_001100"
     CDS             7857..8870
                     /locus_tag="VGY24_001100"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ4284083.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQSALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            8857..9846
                     /locus_tag="VGY24_001101"
     CDS             8857..9846
                     /locus_tag="VGY24_001101"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ4284084.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVVDNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQRVN"
     gene            complement(10076..11353)
                     /locus_tag="VGY24_001102"
     CDS             complement(10076..11353)
                     /locus_tag="VGY24_001102"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ4284085.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDRDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(11340..12515)
                     /locus_tag="VGY24_001103"
     CDS             complement(11340..12515)
                     /locus_tag="VGY24_001103"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ4284086.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
     gene            12724..>13431
                     /locus_tag="VGY24_001104"
     CDS             12724..>13431
                     /locus_tag="VGY24_001104"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ4284087.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQP"
BASE COUNT         4248 a         2333 c         2653 g         4197 t
ORIGIN      
        1 acattatggc gcttcttttc tattattatg tgaggaaaaa tcaagtaaaa aagttaagtc
       61 aactgaaaaa cttgacagaa atattcagaa aatttataat actaactatt aacaacatat
      121 cagctattaa agcatttcaa acagcaggtg ttttatttgg acctgttaag gctgcaaatg
      181 caagagaaag cagtatctgg tttgaaaatc tcacaaagca ataactatct ctcttagaca
      241 tttgaagaag cagacagtaa attcgataat attgtgatga gaacttagca acaatctgct
      301 gatgcaggtc aatttttatt tggtactcat attgcagaat ttcataaagt tgcccaagat
      361 aacttgaaat aaggaatcac ctgatgtttt aaacagatga tactaagtct atcatatggt
      421 gataatagtc cattgaacat gtagtttatg aattaaaaag aaggtttaag gagataagaa
      481 tgattagaga aatcataaca gatcattttt tattacagca aaaagcgcaa gtcgccaaga
      541 aagaagatct atggattggc caagatttac aagatacctt agccttttat agacaggaat
      601 gtcttgggat ggctgccaat atgattggtg aacaaaagcg cattgttatt gtgagtatgg
      661 gatttattga tttggtgatg tttaatcctg tgatggtttc taaaaaggga atttaccaaa
      721 ctaaagaatc ttgtttgtcg ttatctggct atcgcaaaac acagcggtat gataagatta
      781 ctgttgagta tttggaccat aattggcgac caaagcgatt aagcttaaca ggcttaactg
      841 ctcaaatatg tcagcacgaa ttagatcatc tagaaggtat tttaatttaa caaaaaacct
      901 agagtaggac gctttaggtt tttttggcaa agatgcctga ttatataagg gtgatcgatt
      961 actagtaaac gctaggattt ccttttttta ataaaagcgt ttagtccacc taagcttatc
     1021 aaggcaagga ttgtcataat aggagacatt ttataattgt cccctgttat tggaagagtg
     1081 gaactagaga tagtattttt gtttgtcgtt gtttcttttt tgccaaggct gttattagtg
     1141 ttttcagtag aagtcaaaag gtctgaaatg acttcactag acactatatt gtcatcacga
     1201 tttctaaaaa ccttactaat gatactgtgt ttaccagcac tattaacttt tgtcgaactt
     1261 tcaaggtttg aagttacata atttttaacc ccttttatag tagcattaac agttttacct
     1321 gatgcttcta aatttgtgat atatgtgatg aaagcttcag tatctgtgtt aatagctccg
     1381 attaatttag cttttttaaa tgctgaaaaa ccatcaccac caccataaag aaagtcgttg
     1441 acaacaacgg tgtaagtagt tgttaagtta atttcttcac cattgtcttt ataaacctta
     1501 actatcttga agggggtatc agagttctta ggatcattat ctgtataagt gtatgttaaa
     1561 cctgacattt gaagaaaata ggtctggttt tcatcgtatt gctgatttag gacatcgtaa
     1621 atgtgttgac cagtcatttg aatgacttga aggatattac caaatggttg tacagcctgt
     1681 gcagctcccc aggtgatggt ccggtcattt ttgacaacta ggtcacttcg aataccacca
     1741 ttattggtca tagcaaagtc aacagttgga aaagttttct tagcaatagt aagctgagcc
     1801 gttgttacta agttaccgac aggagattct ttatcaatat tttctgtttt agaaatagtt
     1861 gaagaattag ttgcagttcc gatttttcgt tcagtaactg ttttaacaat atcattagca
     1921 tgatttatta tagctttgat atctgaattt tctgttttga tacctggtgc tacagtaaca
     1981 acatttgctg atggagttgt cataaaatca ttggtatcag tatctagcgt tccacggaca
     2041 tctgcataag cttttccttg agagagggct tgaacgatac gtgttttacc gatagttcca
     2101 ttagtgtatt gatgattatg tcctgcaaaa ataatatcaa tgctatgttc gggatagatt
     2161 tggttcactt tttccataac cgtagccatt tcatgatcaa caacaccatc tttacttgtt
     2221 gcaggaacat gagctaaaac cacaatagca tgaacatgtt gttcttgtag ttctttagca
     2281 tatttggcaa tggtttcagc tacatctaaa aattgatagt gttcatagtt ttgctttaaa
     2341 acgagatttg gaatctctgt agtcacaaca ccaatgaagc caatcttaac gattttgtca
     2401 ttaatggcta tgtcttttat agcataaggt ttccaaccat agggtatatc attggttttt
     2461 ttatcaataa cattagcaat gacgatggtt tgatgcgaag cttcgtgctc atattgtttg
     2521 gtgatatcat taattgttga ttcaggatca ggcgcttgac ctgtcatgat acggttaaat
     2581 tcatctagtc cttcgtcaaa ttcatgatta ccaagagtgc catattcaaa tttcattttg
     2641 ttaaagactt ttacagtagg ctcatcttgt aaaagtgcag agttagcagg actggctccg
     2701 accatatctc cagcttgaac acgtatactt gttccgtctt gatttgcttg cttgaagtct
     2761 atctcagcgt catccatata agcacctaat tgagcagccg tcccagcatt tggtatttta
     2821 ccacttggtg tgtaagctgt tccggtatta tcaagagcgc cgtgaaaatc attgacgcca
     2881 aggaattgca catcaacttg atctgcttga acgtctgtaa ctaatagagt aaaactagtc
     2941 aggatactca atacactact ttttaaaata aaatattttt tcataaaatc tcctaataag
     3001 ttaacgtaat cgttatagat caagtttact atgttttcgt ttgtaattca atggtatttt
     3061 taaaaaataa cgcacaaaaa atatatgaaa gcgatttaaa aacaaacgat aaagatcgtt
     3121 tacagttata tagttagaaa taaagataac cgatcattgt agagatttaa gcatttgttg
     3181 agaaaatttg ttataataag cgtatgaaag aaagactaga acattattct accagctcta
     3241 tatatagcgg atttgaagtg tacttgccgt tggtactaca gacaataaca gatgtgatta
     3301 ttgctgagaa tatcaaaagc aaaaaagaaa cagggtttaa actatatgaa cactttacat
     3361 cccgtattaa atcggaagct tcgatgattg aaaaatgtca acgcaaacaa cttcctctaa
     3421 caagcaaatc tgcgttaaaa atcattaaag atagtatcgg tattcgcatt atttgtggtt
     3481 ttattgatga tatttatcgg atggtagact tgctcaaaag cattcctggt atgtcagtta
     3541 acactgaaaa agattatatt ttaaatgcca agcctaatgg ctatagatca tatcatctca
     3601 ttttggaatt agaaacacat tttccagata ttttagggga aaaaaaagga tgttatttta
     3661 tagaagtgca gttgcgaacc attgctcaag actcttgggc tagcttggaa catcaaatga
     3721 agtacaaaca tcaagttgct aatgcagaaa tgattacaag agagttaaag cgttgtgcag
     3781 acgagttggc atcttgtgat gttaccatgc aaaccattcg tcaattgatt caagaaacga
     3841 cggaggaaga gtaaccatga aaattttgtt agctgaagac gaatggcaga tgtctaatgt
     3901 tttgacaact gccatgactc atcaaggtta tgacgttgat gtggttttta acggtcaaga
     3961 ggcaattgat aaagccaaag acaatgcata tgatattatg attttagata tcatgatgcc
     4021 gattaagtct ggcattgagg ctttaaagga gattcgcgct tcaggaaatt gctcacatat
     4081 tattatgctg acggctatgg cagaaattaa tgaccgtgtt accggactag atgcaggagc
     4141 tgatgattat ctgactaaac ctttctcatt aaaagaactt ttagctagat taaggtcgat
     4201 ggaaagacga gtagaaagtt ttactcctca ggtattacaa tttgctggtg tcacattaaa
     4261 tattaatgag caagaattga gtgccggtaa tgctatacga ttagcttcta aagaaggtaa
     4321 attaatggcc tttttaatgt taaatcaagg aaagtacttg gatactaaaa cgctttacca
     4381 acatgtttgg tcagaccaag aagattatga tacttctatt gtttgggttt atatttctta
     4441 tcttcgtcaa aaattattag ctattcaagc aaatgtcatt attgcaggtg ataaagatac
     4501 tagctattgt ttattagaaa aataggaggt gacatgttta atcgtatccg tattcgtttt
     4561 atcatgattg cttctattgc catttttatt attttaagtt caattgttgg tattattaac
     4621 acggcacgct gttaccaaag tcaacaagaa attaaccgta ttttacattt aatttcctca
     4681 aataaaggta aattacctgg cactacggaa agttctaaac gacttggaac gaaactgtct
     4741 gaagatagcc taagccagtt tcggtattat agtgtcattt ttaatgctaa tggacatcta
     4801 ctttcttcta atactgctaa tatttcagct ttagatagag aagaagccca atattttgct
     4861 agactttttg ctaagtctgg ggaagaaaaa ggcagttacc gtcaccaaga tagtgtttat
     4921 tcgtacttga ttactcagct gccaaatgaa gaaaaactgg ttgttatttt agatactacc
     4981 ttttattttc gtagtgtagg agatttatta gctgtttcaa tgatgttggc ctttggagga
     5041 tttatctttt ttgttgtttt agtgagtctt ttttctggta tggtcatcaa accttttgtc
     5101 caaaattatg agaagcagcg ccgctttatt actaatgcag gccacgagtt aaaaacacca
     5161 ctagccatta tctcagccaa taatgaattg gtcgaattaa tgactggaga atcagaatgg
     5221 accaagagta caagtgatca ggtgaagcga ttgacaggct tgattaatca aatgattact
     5281 ttggctcgtt tggaggaaca gccagatgtg gtattacata tggtagactt ttcagttatt
     5341 gctcaagatg cagctgaaga ttttaagagt ctggttttga aagatggtaa acgttttgat
     5401 ttgacgattc agccgaatat tatgattaaa gcggaggaaa agtcattatt tgaacttgtg
     5461 acaattttag ttgataatgc caataaatat tgtgatccta aaggcttggt caaggtatct
     5521 ttaaccacta ttggaagaag acgaaaacga gcgaaattag aagtttctaa tacctatttg
     5581 gaaggaaaat ccattgatta cagccgcttt tttgaacgct tttatcgcga agacgaatcc
     5641 cataatagca aagaaaaagg ttatggaatt ggtttatcta tggcagaaag tatggttaag
     5701 ttatttaaag gaacgataac tgtaaattat aaaaacgatg ctattgtttt tacagtgatg
     5761 atttgactta aacatttttc agtaccttta gctagtaata atgtctaggt aaaggtaata
     5821 aacgatgctt aaccagccca tgaggctggt ttttaacgtt taatttagta gaagagtgat
     5881 taaagaaggt agtttgaaat aagaaaattt ttgttatgat cctaaaaaga gtagatagtc
     5941 tttattgtta atttcggtga agaaatgatt ttaaaaagat atatcatcgc attttctaat
     6001 catttagtgt aaaatagtaa ggtaaaagtt tagaagaatt agtaatgaaa agataaaaaa
     6061 tgaacgaaaa cattggatat ggtaaggcac acagtaagat cattttgata ggtgagcatg
     6121 ctgttgtgta tggctaccca gctattgctt tgcctttaac agatattgag gtggtttgtc
     6181 atatttttcc agctgataag ccattggtct ttgattttta tgatactcta tcaacagcca
     6241 tttatgctgc cttggattat ttgcagcgac tacaagaacc aattgcttat gagattgttt
     6301 cacaagtgcc acaaaagcgt ggtatggggt cttcggcagc cgtttctatt gcagctatta
     6361 gagccgtttt ttcttattgt caagaacccc tttcggatga tttgttggaa attttagtca
     6421 acaaggcaga aattattgcc cacaccaatc ctagtggttt agatgccaag acatgcctta
     6481 gtgaccatgc cattaagttt atccgaaaca ttggctttga aactatcgaa atcgccttaa
     6541 atggttatct tatcattgca gatacaggga ttcacggtca tacacgtgag gccgtcaata
     6601 aggtagcaca gtttgaggaa acgaatttgc cctatctggc taaactagga gctttgacac
     6661 aagcccttga aagagcgatt aaccaaaaaa ataaggtggc tatcggtcag ctcatgacac
     6721 aagcgcactc tgctttaaag gccattggcg ttagcatcag taaagctgac caacttgtgg
     6781 aggctgccct aagagcaggt gctttaggcg ctaagatgac aggcggtggc ttgggtggct
     6841 gtatgattgc cctagcagat accaaagaca tggccgaaaa aatcagtcac agattaaaag
     6901 aagaaggagc cgtaaacacg tggatccaaa tgttataacc gtaacatcct atgctaacat
     6961 tgctattatc aaatattggg gaaaagagaa tcaagctaag atgattcctt cgacctctag
     7021 catttctttg actttggaaa acatgttcac cacaaccagc gtttccttct taccagatac
     7081 tgcaaccagt gatcagtttt acattaacgg tgtcttgcaa aatgacgaag aacataccaa
     7141 aatttctact atcattgacc aatttcgcca acctggtcag gcttttgtaa agatggaaac
     7201 tcaaaataat atgccaacag ctgcaggttt atcatcaagt tccagtggct tatcagcctt
     7261 ggttaaagcc tgtgatcaat tatttaacac ccagctagat cagaaagctt tagctcaaaa
     7321 ggctaagttc gcctcaggat catcttctcg ttcttttttt ggcccagtcg ctgcttggga
     7381 caaagatagt ggtgctattt acaaggttga gactgacttg aaaatggcca tgattatgct
     7441 ggtcttaaat gctgcgaaaa agccaatttc tagccgagag ggcatgaagt tatgccgcga
     7501 tacctcaact acatttgatc aatgggtaga acaatcggca atcgattacc aacatatgct
     7561 tacctatctc aaaactaata attttgagaa agttggtcag ttaacagaag ctaatgcctt
     7621 ggccatgcat gccacgacaa agacagccaa tcctcctttt tcttacctaa caaaagagtc
     7681 ttaccgggct atggaggctg tgaaagaact gcgtcaagaa ggttttgctt gttattttac
     7741 tatggacgca ggtccaaatg tgaaggtcct atgtttagaa aaagacttgg ctcaactagc
     7801 agaacgactt ggcaagaact accgtattat cgtttcaaaa acaaaggatt taccagatgt
     7861 ctaattattg tgtgcaaaca ggtgggaaac tatacctcac aggcgaatat gctatcttaa
     7921 caccaggaca aaaagcctta attcacttta ttccactgat gatgacagca gaaattagcc
     7981 cagcagccca tattcaatta gcttcagata tgttttccca taaagcgggc atgacacccg
     8041 atgcctctta tgcactgatt caagcaacgg ttaaaacctt tgctgattat ctaggacagt
     8101 caattgacca actggagcca ttttccctaa ttataacggg aaaaatggag cgcgatggca
     8161 aaaaatttgg cattggttca agtggtagtg tcaccctctt aaccttaaag gccttatcgg
     8221 cctattatca gatcacttta accccagagt tactctttaa actagcggct tataccttgc
     8281 ttaagcaagg agataatggg tctatggggg atattgcctg tatcgcttac cagactttag
     8341 tcgcctatac ctcctttgac cgagaacagg tcagtaattg gctgcagacc atgcccttaa
     8401 aaaaacttct cgtcaaagat tggggttacc atatccaagt cattcaatca gccctgcctt
     8461 gtgacttttt ggttggctgg actaaaatac ctgctatttc aaggcagatg attcaacaag
     8521 tgacagcgag cattacccca gctttcttaa gaacaagtta ccagctaacg caatcagcta
     8581 tggtagcttt gcaagaaggt cataaggaag aactcaagaa aagtttagca ggagcaagtc
     8641 atctcctaaa agagcttcat ccagctatct accatcctaa gctagtaacc ttggtagctg
     8701 cttgtcagaa gcaagatgct gttgctaaat cttcaggttc tggtggtgga gattgtggca
     8761 tcgcgcttgc ctttaatcag aatgccagag atacccttat ttccaaatgg caagaagctg
     8821 atatcgcatt actttatcaa gaaaggtggg gagagaatga ccaaccgtaa agatgatcac
     8881 atcaaatatg ccctcaagta ccaatcgcct tataatgctt ttgatgacat agaactcata
     8941 catcattcct tacctagcta tgatttgtct gatattgatc tcagtactca ttttgccggg
     9001 caagacttcg actttccctt ttacatcaat gccatgacag gaggaagtca aaaaggcaaa
     9061 gctgtcaatg aaaaattggc caaagtagca gcagcaacag ggattgtcat ggtgacaggg
     9121 tcttatagcg ctgctttaaa aaatcctaac gacgattcct atcgtttaca tgaggtggta
     9181 gataacttga aactagccac gaatattggt ctagataaac ctgtggcgct aggacaacaa
     9241 acggttcaag aaatgcagcc cctcttttta caggttcatg tgaatgtgat gcaagagttg
     9301 ctgatgccag agggtgagcg cgtctttcat acctggaaaa aacacctcgc tgaatacgct
     9361 agtcaaatac cagttcctgt cattctcaaa gaagttggtt ttggcatgga tgtcaatagt
     9421 atcaagctag cacatgacct aggcattcaa acctttgata tttcaggaag aggagggacc
     9481 tcatttgcct atattgaaaa tcaaagagga ggagaccgct cttacttaaa cgattgggga
     9541 caaaccaccg ttcagtgctt gctaaatgca caaggactga tggaccaagt ggaaatctta
     9601 gcttcgggtg gtgtcagaca ccccttggac atgattaaat gtttcgtcct aggagcacgt
     9661 gcagtgggac tctcacgcac cgttttagaa ttggttgaaa aatacccaac cgagcgtgtg
     9721 attgctatcg ttaacggctg gaaagaagaa ttaaaaatca ttatgtgtgc tcttgactgt
     9781 aaaactatta aagaattaaa gggagtcgac tacttactat atggacgctt gcagcgggtc
     9841 aattaggctt catcacctct acaatagtgc cacctactac aaagatgata gagctttttt
     9901 agaacataat ataaaatatt tataaatacc tcatcaaact cagaatagag tagatgagta
     9961 aatagagtat gttatggaga ctatttctaa gacttgtatg tatagaaaca aagattaaac
    10021 acccatgtaa accaattgtt tacatgggtg attgccttat attagttgtt taaatctaag
    10081 actgtcttag ctgagataag taaaaatgtg ctttttctag atttattggt ttatcagcca
    10141 gtaaagcatt aactaaagga gcaatttcgt cttgggttgc ccccgccaaa agagctaatg
    10201 atttcgcatg tagtttcatg tgaccagctt ggatgccaga tgaggtcaga gcttttagtg
    10261 cagcaaagtt ttgagctaac cccacagatg caatcaattg ggctaacgtt ttggcatcag
    10321 gttgattaag aagatcatgt gcgatagaaa cagtagggtt aagcccgata gatcccccct
    10381 tactagcaat aggcataggg agggtcattt ggccaagcag aacttgttta tcttggtcaa
    10441 aatgccactg actaagtcct tgatagctac catttcttga ggcataagca tgtgcccctg
    10501 cttcaatagc acgccagtca tttcctgtgg ctattaccac tgcatcgata ccattaaaaa
    10561 tacctttatt atgagtagta gcccggtaag gatctacttg agctagtcgg ctggctagct
    10621 ctattttttg agctaactgt agggattttg ttttatcgcg atctaaaagg cgcacgggaa
    10681 tctcacaact agtagtaaca agcgactctg ttgcgtaatt agataaaatc gccattagac
    10741 aatgcccctt agacagtctt gttaaatcag gaacaagagc ttccatcata gtattgacca
    10801 tatttgctcc catagcttct tgggtatcaa cagtcagata gaaaatcaaa aactctcctt
    10861 tttcttccaa atgaatggtt ctagcgcctc cacctctttt aacaatggaa ggataagctt
    10921 tatttgccaa tgcaattagc ttttttgttt tatgaaggat ggcggcttta gcgttatcta
    10981 tttggtcgat atcgtaaaga acaatttgac caatcatttg tcggtttagg gtttgagctt
    11041 taaaaccacc tgagcgtttg attagttttg ctgcgaaaga tgctgcagca acaacagaag
    11101 gttcttccgt gacaaaaggc atctgataag ttgagccgtt gacaagaaaa tcaggagcta
    11161 tgctaaaggg caaagccaat cgtcctaaga cattttcagt catttgatta gctgtttgga
    11221 taggcaaaag aacgtctgtt ttgagttgat ttaagttttc agcattaagt agtttaaatt
    11281 tttcgataag ttggaggcgt tcttcgaatg ttttctttga aaaaccgctc cagttaagat
    11341 tagtttttgt cattggtatg atagatcctt cgatgctcct tgatggctgt tagagcaaat
    11401 tttccagtta agtaaatgtc aaaattggca ttaccattat catctaattg gacttgttcg
    11461 tagaaaaggt cttcgtattt agaaacggaa agacgtgttc gttgatccag taaagcttgt
    11521 cgattagtca ttaacatttt atcgtaccca gcaactaact gaccggaata aaattcactt
    11581 acggcaccgg aaccatagct aaaaaaacca attttatctc cagattgtaa tactttacta
    11641 ttttcaagta aagataacaa tcctagataa agtgagccag tataaatatt tccaatttgt
    11701 ttgctataag taatagaagc ttgaaaggct tctattagtt tttccctgtg ttcaggaggt
    11761 actgtgttat ccataatgtt atttagacct tttagggcta acttgggaaa tggaatatgg
    11821 aaacaaactg cagcaaaatc agataactga aggttttctc ttttctgata agcttgccac
    11881 gtggtttcta agcaattgag atattgcttg gtagagtaaa taccatcaac ataaggtgtg
    11941 aaactatagt taggcctcca aaagtccata atatccctag tttgagccac attatcttta
    12001 tttagagcaa gaatacgagg gttagcagtt accaaaagag caatactacc agagccttga
    12061 gttgattcgc caggagatcc tacaccgtat ctagcaatat cactagcaat aacaaggaca
    12121 cgagaatctg gcttagaagc cacatgcagt ttagcgtagt ctagagcagc agttgcgcta
    12181 tagcaggctt gttttacttc aaaggaacgt gcaaaaggct ggataccaac taagtgatgt
    12241 acatagatag ctgatgcctt tgactgatca gtactcgatt cagttgccaa aataaccata
    12301 tcaatcttag atcgatcttc gtcggttaag atttgatctg ctgcagaagc agctaaagta
    12361 ataatgtctt cagtgattgg tgccacacta aaagactcaa tgagtagccc ttgactaaat
    12421 tttgctggat ccacttggcg tgcaagcgct aaatcttcca atttcaagac atattgactg
    12481 gttgcaaaac caatcttatc aattccaatt gtcatatttg tccttctttt agttattttt
    12541 atagtcttac ccattctatc acattttact gcaaaattag actgataaag ttatttgttt
    12601 tatcctttaa tagttaaaat gttcatgacc acatgaagtt tttcgaatgt ttatttattc
    12661 tggtattttt tggtaaaatt agttagaata gtatgaagta cgcttacaga aaggttatta
    12721 gagatgacaa aagctgacca gatttttaaa gctaatattc aaaaaattat aaacgaagga
    12781 tcattgagtg agcaagcacg tcctaaatat aaagatggtc gaacagctca ttctaaatat
    12841 attactgggg cgtttgctga atatgattta gctaaaggag agtttcccat cacaacttta
    12901 cgacccattc caattaaatc tgctataaag gaattatttt ggatatatca agatcagtct
    12961 aacagtttag atgtgctaga agctaaatat aatgttcatt attggaatga atgggaagtt
    13021 gaccagacac gtactattgg acaacgttat ggtgctgtag tcaaaaaaca tgatatcatc
    13081 tcaaaaatac taaagcaatt agcagaaaat ccttggaatc gtcgtaatgt catttctctc
    13141 tgggactatg aagcatttga ggagacaaaa gggttgttgc catgtgcctt tcaaatcatg
    13201 tttgatgtta gacgtgtagg ggaagatctt tatttagatg ctagtttgac gcaacgttct
    13261 aatgacatat tagttgctca tcacatcaat gccatgcaat acgttgcctt gcagatgatg
    13321 attgctaaac attttggatg gaaaataggt aagttctttt attttgttaa caatttacat
    13381 atttatgaca accaatttga tcaggctcaa gaattactaa aacgacagcc a
//
LOCUS       DAUCWU010000042        13387 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUCWU010000042 DAUCWU010000000
VERSION     DAUCWU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.042"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..113
                     /locus_tag="VGY24_001105"
     CDS             <1..113
                     /locus_tag="VGY24_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ4284088.1"
                     /translation="VLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI"
     gene            193..690
                     /locus_tag="VGY24_001106"
     CDS             193..690
                     /locus_tag="VGY24_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ4284089.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            710..880
                     /locus_tag="VGY24_001107"
     CDS             710..880
                     /locus_tag="VGY24_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284090.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLGSLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            1010..2239
                     /gene="clpX"
                     /locus_tag="VGY24_001108"
     CDS             1010..2239
                     /gene="clpX"
                     /locus_tag="VGY24_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ4284091.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            2249..2848
                     /locus_tag="VGY24_001109"
     CDS             2249..2848
                     /locus_tag="VGY24_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ4284092.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESMVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQI"
     gene            2996..3739
                     /locus_tag="VGY24_001110"
     CDS             2996..3739
                     /locus_tag="VGY24_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284093.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(3797..5896)
                     /locus_tag="VGY24_001111"
     CDS             complement(3797..5896)
                     /locus_tag="VGY24_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ4284094.1"
                     /translation="MAHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSESNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQKDQ"
     gene            6274..6957
                     /gene="rpiA"
                     /locus_tag="VGY24_001112"
     CDS             6274..6957
                     /gene="rpiA"
                     /locus_tag="VGY24_001112"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ4284095.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPKAS"
     gene            7034..8245
                     /locus_tag="VGY24_001113"
     CDS             7034..8245
                     /locus_tag="VGY24_001113"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ4284096.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLSHLK"
     gene            8264..8704
                     /gene="arsC"
                     /locus_tag="VGY24_001114"
     CDS             8264..8704
                     /gene="arsC"
                     /locus_tag="VGY24_001114"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ4284097.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            8688..9497
                     /locus_tag="VGY24_001115"
     CDS             8688..9497
                     /locus_tag="VGY24_001115"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ4284098.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENPVV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTEHIKEDFKGLVKAILAEL"
     gene            10082..10795
                     /gene="deoD"
                     /locus_tag="VGY24_001116"
     CDS             10082..10795
                     /gene="deoD"
                     /locus_tag="VGY24_001116"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ4284099.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            10788..11576
                     /locus_tag="VGY24_001117"
     CDS             10788..11576
                     /locus_tag="VGY24_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ4284100.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWINQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWQFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(11656..12561)
                     /locus_tag="VGY24_001118"
     CDS             complement(11656..12561)
                     /locus_tag="VGY24_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ4284101.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLSDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            12783..13319
                     /locus_tag="VGY24_001119"
     CDS             12783..13319
                     /locus_tag="VGY24_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284102.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKIPTGEFE"
BASE COUNT         4086 a         2373 c         2754 g         4174 t
ORIGIN      
        1 ttgttttaaa tgttccagat gggactaatt tctttgatat taaaccagat gattttgaat
       61 tacaaaatta tgatccagtg aagcctcagt tgcactttga ccttgctatt taaactttat
      121 ttagattttt tgtgagcatt ttgttcataa atttggtaaa ataaatagag tttaaaatga
      181 aggaaataat taatgacaaa ggaaattatt gctatttggg cagaggatga agcagggctt
      241 attggtgttg cgggtaagtt accatggtat ttaccaaaag agttagaaca ttttaaaaag
      301 acaacgctac atcaagcaat cttaatgggc cgtgtgactt ttgaaggaat gaattgcaaa
      361 agacttcctc aacgtcaaac ccttgtcatg acaagaaata gagattatca agtagatgag
      421 gtgttgacaa tgactagtat tgaaaaagtc ttggagtggt accatgcgca ggataaaaca
      481 ctttatatca ttggtgggaa taaggtttta gaagcgttta atggttattt cgatcgtatc
      541 ataaaaacag ttatccatca tcgttttaaa ggagatacct accgaccaaa tctagatttt
      601 agtcatttta cgcaagaaag ccaaacgttc tatgctcgag acgctaaaaa tccttatgat
      661 tttaccgtta ctgctttaaa acacaaatag tctgaggaga tgataagcga tgcaacgtag
      721 tatttttgga gtatttacgg cttttttgtg cgtgatttgt gttctttgtg ccatcccagc
      781 ttttaaaaag aatcgttatg gcttaggaag tctgttctta ttaaatgcgt ttactaatct
      841 ggttaatagt attcacgcct tctatggtac attgttttga gatgcgactt gcaaagacag
      901 cactggtgaa gatttagcta tcaagcaaga atcaccaaaa accaatacaa cacattgact
      961 aatattaaaa ttcagtaaaa gataagagaa agtaagagaa ttataagaaa tggcaggaag
     1021 tagaactaac gatattaaag tatactgttc attttgtggt aaaagccagg atgacgttaa
     1081 aaaaattatc gcaggaaata atgttttcat ctgcaatgaa tgcgtagctt tatcacaaga
     1141 aattataaaa gaagaattgg ctgaagaagt ccttgccgat ttgacagaag ttccaaaacc
     1201 aaaagagctc cttgatgttt taaatcaata tgtggttggt caagatcgtg ctaaacgtgc
     1261 tttatcagtt gctgtgtaca atcattataa gcgtgtctcg tttacagaaa gtcgtgatga
     1321 cgatgatgtg gatttacaaa agtccaacat tttgatgatt ggaccaacag gttcaggaaa
     1381 aaccttctta gcacaaactc tggctaaaag cctcaatgta ccatttgcta ttgcagatgc
     1441 aacttctttg actgaagcag ggtatgttgg agaagatgtg gaaaatattt tactcaaact
     1501 gattcaggct gctgactata atgtcgaacg agcagagcgt ggtattattt atgttgatga
     1561 aatagataag attgctaaaa aaggtgagaa cgtatctatc acacgtgatg tatctggtga
     1621 aggtgtacag caagcccttc ttaaaattat tgaaggtacg gtagcaagtg ttcccccaca
     1681 aggtgggcgg aagcacccta atcaagaaat gatccaaatt gatacaaaaa atatcttgtt
     1741 tattgttggc ggggcttttg atggcatcga ggagattgtt aaacaacgtt taggtgaaaa
     1801 agttattggt tttggtcaaa acagccgtaa aattgatgac aatgcttctt atatgcaaga
     1861 aattatttca gaagatattc aaaagtttgg gcttattcca gaatttattg gccgcttacc
     1921 agttgtggca gcccttgaac aactaaacac atcagatttg attcaaattt tgacagaacc
     1981 aagaaatgcc ctggtcaagc aatatcaggc ccttctttct tacgatggcg tcgaattggc
     2041 atttgataag gaagccttag aagccattgc caataaagcc attgaacgta agacaggagc
     2101 acgtggtctt cgttctatta ttgaagaaac aatgcttgac attatgtttg aaattccaag
     2161 ccaagaagat gtcacaaaag tgcgtatcac caaggctgct gtcgaaggca agtcaaaacc
     2221 cgttttagag acagcttaag gagaaataat ggctgaagaa caagtcttaa atacccataa
     2281 cgcctcaatt ttattgagtg ctgccaataa atctcattac ccccaagatg atttgccaga
     2341 aattgccctt gctggacgtt caaatgttgg taaatcaagt tttattaata ccatattggg
     2401 gcgcaaaaat ttggcaagaa cctcaagcaa gccaggaaaa acccaactat taaatttttt
     2461 caatattgat gacaagctta gatttgtgga tgtccctggt tatggctatg ccaaggtttc
     2521 taaaagtgaa cgggccaaat ggggaaagat gatcgaagaa tacttaacca gcagagacaa
     2581 tctgcgtgca gtcgtgagtt tggttgattt acgtcatgcc ccttcaaaag aagatattca
     2641 gatgtatgac tttttgaagt actatgacat tcctgttatt gttgtggcaa ccaaagcaga
     2701 taagattcct cgtggcaaat ggaacaaaca tgagtctatg gtcaagaaag ctttgaattt
     2761 tgacaaatct gatactttta tagtattttc ttcggtagaa cgtattggta tcgatgattc
     2821 ttgggatgct attttagagc agatctagcg ataggtgttg atttatctat gctaaaacat
     2881 tctcagtaga gggtgttttt cttatcgaaa acataaaaaa tcatagttag gtttaccgat
     2941 tttacttgac gccttaatga tgacatgcta gtattttata ttaaggagat gagtcatgac
     3001 acaagtagcc caagggcgct ctaaaggaaa gtcattgtta tgccatcaat gcaatgccct
     3061 ttttgtattt attatttttc ttatcttgct tgctgtactt tttggtgttc gatatcgtaa
     3121 tagctctatt gaaggtatct ggcgaacaac aagcattgat cagaagttag gcgatgattt
     3181 tgctaaacgt cttactggtt tgcatcagag ccctcttatt gatgatagtt tattaacgtc
     3241 atctcagatg atactaacag ttaaaaataa caacgttgac ttgagtttta gtgttcaagt
     3301 tgaacgagac atttttgtca aacgtttagc tgcttatcac caaaatgaat tattaaagac
     3361 attaaaggaa aatcatttag ttgttggtga cttgagtagt aaagaacgcc aaattattga
     3421 gaatagtatg ccagctagcc atgaattaga aatgatttta gatcaagctt ttgagaaatt
     3481 agcttctcag atcggaggca aatataatca aaaaacagga catttatctg ctgttgttct
     3541 caaaggaaaa gttaatcgta ttttgcacac cattgatatc aaagaagaag tagcagcggg
     3601 acatacttct ttttcaaaag gattgctcac acctaatggt tattttgatt acaccagatt
     3661 tggaaagaaa cttgagttat taggcgatga aaaaattatt tttaaaaaag cgctaaaaaa
     3721 aagtccgtca agtgtataac gtagtcacaa gacggatttt ttcatgtttt gaaaattatt
     3781 caattgaaga agtttattac tgatcttttt gcctaatcac tagttgtcct tcttttagga
     3841 ttgcctgaag ttttttgact tctggatggt caagataata atcagttata cggtcacgaa
     3901 tttcctgttc gataatacgt cgtaatggac gagctcccat agcgtgattg taccctaaat
     3961 ctatcaaatg agctttagcg tcatcagcaa tcgttaaact aatacctttt tttgcggtgg
     4021 tttgatttac ttcagctagc attaaatcaa caatttcttc taaattttct ttttgcagat
     4081 gattaaattt aatgatacta ttaaaacggt tcaaaaactc tggtctgaaa taaggtgcaa
     4141 tgcgatccat aatgttagac tcagaagttt ccgtatcttg ttggccaaaa ccggcatttg
     4201 atgtagcaat aataacggta tttttaaagt taatagtatt tccttgtcca tctgtcaatc
     4261 gtccatcatc taacacttgt agaagaagtg taatgatttg aggatcagct ttttcaattt
     4321 catctagtaa gacaatagca taagggttac gacgaacacg ctcagttagg gtattattat
     4381 tatcatcgta gccaacataa ccagccgttg tcccaatcaa ttttgacaca gcagtgcgat
     4441 cgttatattc ggacatatca agtcgaataa tagcatcttt ggaaccaaat aaatcaagtg
     4501 ctaattgttt agctaactct gttttaccaa caccagttgg acccacaaaa aggaaagaac
     4561 ctattggacg gttaccatca tcaaaacctg ctcgatttcg tcgaatagca cgtgctactg
     4621 ctgcaacagc ctcatcttgt ccgatgacat ggctttttaa gcggctgcta attcctttga
     4681 gcctttctaa atcattagtc cccatgttag agacgggaat accagttaag cgctctacag
     4741 actctgcgat atcattgact gtagcagtaa cattttggct ttggttatgt tgctcaattt
     4801 gcttttgtaa tgtttcaatc cgtgtttttg ctgctaaagc tttttcaaag tcttctttgg
     4861 cgacagcttt ttcttgcctc tctttttgtt tagctatttc agtttcgagt gtttttaagt
     4921 cagtgaccgg gtgttgcgca gctaaatgag cagctgtcat atccagtaag tcaattgctt
     4981 tatctggcag gctacgctgt gggatgtatt gaatagaata atcaacagca gcttttagaa
     5041 cattatcagg aagggtaata tggtggtgtt gttcataaag attacgaata cccattaaaa
     5101 tgtgaaaagt atcttcagct gatggtgcat tcactttaac ttcattaaag cgacgagcga
     5161 gtgcagcatt ttttaaaata gtattgcggt attcgtcttg agtagtagca ccaatcagtg
     5221 ttaattctcc tcgtgacaag gctggtttaa gaatatctgc aagtcccttt gagcctgaat
     5281 cactgcttgt ggcaccagca ccaactattt gatgaatttc atcaaaaaat aagatgatat
     5341 tcccagcttc ttttacttct tgaatcaagt tttgaatggt ctcttcaaag gaaccacgat
     5401 actgtgtacc agcttcaaga ctagaaatat caattgatac gatttccttg tttttaatag
     5461 cagctgggac atctccatta acaatggctt gagctaaacc ttcaatgact gcagttttac
     5521 cgacaccagc atctccaaca aggacaggat tatttttagt tcgtcgtgcc aagatttcag
     5581 cggtatcttg aatttctttg ttacgaccaa tgacggggtc aagatgccct tgacgagctt
     5641 cttgtgttaa gttagttccg agttgagtca atacactatc agctttaggt gttaacattt
     5701 gagaatttgt tgctttggtt gtcgctacag ggagttgtcc tgtttgacga tagtgttgga
     5761 attcttcggg agttatttcg cgaccgttaa cgagatagcg gggattttcg ctacgataac
     5821 cacccatgtt tgccattaat tggttgaaaa tatcatccat atttacaaaa ggatctttac
     5881 cagaaaaatg tgccatatca gttacctcgc aataaaaaat agactataag atgtttgtaa
     5941 gcagtcgcag aatcgttgtt aggatttcac atgataggaa accctttgct aataaaatga
     6001 ttcagacatt atttttactc atcttatagt attattataa tcgaaaggtc agtaaaggtc
     6061 aaacaaaaac acttgatttt caaaaagcta aataaacacg aaaagtaagt gcttatatga
     6121 tgactttaga ataaatcata agttttaaaa agatcatcct attctatagc acttaggctg
     6181 tcaatgagag aaaaacaaat gttttcatta acaagttggc gctttcatgg tataataaag
     6241 tgctaagacg caattctatt ataaggggga atcatggaag cactcaaaaa aattgccggt
     6301 gttaccgctg cgcaatatgt gactgatggc atgaccattg ggcttggaac tgggtcaacg
     6361 gcttactatt ttgttgagga aattggccgt cgcgtaaagc aagaaggact gcaagtggtt
     6421 ggtgtgacta cgtcaagtgt gactagcaag caagcagaag tcttaggcat tcctttaaaa
     6481 tcgatagatg atattgatag cattgacctc accgtagacg gtgctgatga agtggataag
     6541 gatttcaatg gaatcaaggg tggcggcgca gccttactaa tggaaaaaat tgttgcaaca
     6601 ccaacaaaag aatacatctg ggttgttgat gcttctaaaa tggtagagca tctgggtgcc
     6661 tttaagttac cagtcgaagt cgttcaatat ggtgctgatc gtttgttccg tgtctttgaa
     6721 aaagctggtt acaaaccgtc ttttcgtatg aaaggtgata gtcgtttggt gacagacatg
     6781 caaaactata ttattgatct tgatttgggt tgtattaaag atcctgttgc ttttggtcac
     6841 ctgttagatg gaacagttgg tgttgtggaa cacggtctct ttaatggtat ggttgataag
     6901 gtgattgttg ctagtaaaga cggtgttact gttttagaag cgcctaaagc aagctagtct
     6961 tagcatataa cagtgttaat gtattttata ggtattgggg agtcaaccgt aacctcccct
     7021 agattggaga cttatgtcaa aatttaatcg tattcacttg gttgttttag actctgtcgg
     7081 tattggtgca gcaccagatg ctgacaaatt ctttaatgct ggtgtagctg atacagattc
     7141 tgacaccctc ggtcatattt cagaaacagc aggcttatcg gttccaaata tggcaaaaat
     7201 tggacttgga aacatttctc gtccaatacc actaaaaact gttccaacag aagataatcc
     7261 tactggttac gtgaccaaat tggaagaagt atctcttgga aaagatacca tgacaggcca
     7321 ttgggaaatt atgggcttga acattacaga accttttgac actttctgga atggtttccc
     7381 agaagagatt ttaactaaga ttgaagaatt ctcaggtcgc aaaattattc gtgaagctaa
     7441 taaaccctat tcaggaactg cggttattga tgattttggt ccgcgtcaaa tggaaacagg
     7501 tgagttgatt gtttacacct cagcagaccc agtgcttcaa attgcagccc atgaagacat
     7561 tattcctgtg gaagagcttt acaagatttg tgaatatgct cgctccatca cgcttgaacg
     7621 ccctgccctt ttaggccgta ttattgcgcg tccttatgta ggcgaaccag gaaactttac
     7681 acgtacggct aaccgtcatg actatgcagt gtctcctttc caagataccg ttttgaacaa
     7741 actggctgac gcaggtgtcc caacttatgc cgttggtaag attaatgaca tctttaatgg
     7801 ttcaggtatt acaaacgaca tgggacacaa taagtcgaac agccatggta ttgacacgct
     7861 tattaagact cttcaattac ctgagtttac taagggattc tcctttacta acctcgttga
     7921 ttttgatgcc aactttggtc atcgtcgtga cccagagggt tatcgcgact gcttgcatga
     7981 atttgacaat cgcctacctg aaatcatcgc aaatatgaaa gaagacgatt tgcttctcat
     8041 cactgctgac catggtaatg acccaaccta tgcaggcacc gaccacactc gtgaatacat
     8101 tccattgctt gcctactctg catcctttac tggaaatggt cttattccac aagggcattt
     8161 tgcggatatt tcagcaactg ttgcagaaaa ttttggagtt gatacagcca tgattgggga
     8221 gtcattcctt agtcacttaa aataagaaag ttagtagttg ataatggaag aaatcacgat
     8281 ttatcataat ccaaactgtg gcacttcacg taatgttctt gccatgattc gccatgcagg
     8341 tattgagcct actattattg agtatttgca gacgccacct aatcgggaga ctttaattga
     8401 gctcttacaa tcgatgggaa tctctgcgcg agaactcttg cgaaccaatg ttcctgaatt
     8461 tgaggctcat ggattagcaa atcaagcagt agctgagaaa gacgttatta atgccatgtt
     8521 gacggatcca attttgatca atcgtcctat tgttgtgact cgcaaaggag taaaactttg
     8581 ccgtccttct gagaccttac tggatattct tcctgttccc ctgcctagtc cttatatgaa
     8641 agaggatggc gagagtgtga accctattga ggtaaaagga gaaaaagatg tcattgatga
     8701 ctaaaattaa cgaaacaaaa gactttttag ttgctaaagg aattgaagct ccagaatttg
     8761 gattaattct gggttcagga cttggtgaac ttgctgaaga agtagagaat cctgttgtta
     8821 ttgattatgc agatattcca aactggggca aatcaacagt tgttggccat gctggaaaat
     8881 tagtctacgg tgatttagct ggtcgtaaag tacttgccct acaaggtcgt ttccattttt
     8941 acgaaggaaa cccgcttgaa gtggtgactt tccctgttcg tgtcatgaaa gctctgggat
     9001 gtgaaggcgt tcttgtcacg aatgcagctg gtggtatcgg ctacggccca ggtaccttga
     9061 tggcaatcac agaccatatc aatatgaccg gaaataaccc actaatcggt gaaaacttgg
     9121 acgagtttgg tccacgcttc cctgatatgt ctgatgccta tactaaggtt tatcgcaata
     9181 aggcacatga agtggctgaa aaaatgaaca tcaaactaga agatggtgtt tatatgggct
     9241 taactggtcc aacttatgag acaccagcag aaatccgtgc tttcaaagta ttaggagcag
     9301 atgctgtcgg catgtcaact gtgccagaag ttattgtggc tgctcattct ggacttaaag
     9361 tacttggcat ttcagccatc actaactttg cagctggttt ccaatcagaa ttgaaccacg
     9421 aagaagttgt tgaagtgact gaacatatta aagaagattt caaaggtctt gttaaagcta
     9481 ttttagctga gttgtaggac gaacaaaatc aactggagca ttattacagg aatgttattt
     9541 tatgtcgtca gtttggcaca tctgttcgca ataaaaaaat caaaaaataa tcacggggtg
     9601 tgagttctat gaatgttgaa ctcagattct agcttttcta actaacataa ccaacgaagg
     9661 gaaagagacg tattcggttt aaattaagca tggactcgaa catttcttcg tattaatttt
     9721 taaataataa aggaaagaga gttagtcctt tattatttga actcgatttt tagctctctt
     9781 cttctattca atatcaatta tgaaaggaat ggggttgtat tcaattgaat acgggcagaa
     9841 agctcggaaa ctagataaac taacttttgt gctcacacat ggcgttagtt ccctgaattt
     9901 cagttgcttt ctgtccgccc tctgaccttc tacaaaataa aaggaaaggt agggctaact
     9961 gtattcattg aattgaatac gggcggagaa ctgtgtaaaa aagataaact gtctagcatc
    10021 tgggatgcgt cgtcagtttc ctatttttac tttgttcttt gtcgccttta atatcttaat
    10081 aatgtctatc catatttctg ccaaaaaagg cgatattgct gataaaattc ttctacctgg
    10141 agaccctttg cgcgcgaaat ttatcgctga aaatttccta gaagatgcag tgtgttttaa
    10201 cgaagtgcgt aacatgtttg gctacacagg aacttacaaa gggcaccgcg tttctgtcat
    10261 gggaactggt atggggatgc cttcgatctc tatctatgct cgtgaattaa tcgttgatta
    10321 cggtgtcaaa acattgatcc gtgttggtac tgcgggtgcg attgacccag aagttcatgt
    10381 gcgtgaattg gtgcttgccc aagcagcagc aaccaactca aacattatcc gtaatgactt
    10441 cccagaattt gatttcccac aaattgctga ttttggttta cttgacaagg cttaccatat
    10501 cgccagagaa atgggagtaa caactcacgt tgggaatgtg ctttcttctg atgtctttta
    10561 cactaacatg cctgagcgta atatggctct tggtaaattg ggtgttaaag ccatcgaaat
    10621 ggaagcagca gctctttact atttagctgc tcagcaccat gtcaaagctc ttggcattat
    10681 gacaatttct gacaatttga acgacccaac agaagacacc actgctgaag agcgccaaac
    10741 aactttcact gatatgatga aggtcggctt ggaaacctta attgccaatg actaataatc
    10801 aaacgctaga catccttttg gatgtctatg cttataatca cgcctttaga attgctaaag
    10861 ccttgccaaa tatccctaaa actgctctct atttactaga gatgttaaaa gagcgcagag
    10921 aattgaacct tgcctttctg gcggaacatg cagcagagaa tcggaccatt gaagaccagt
    10981 accactgttc attatggatt aaccaatcgc ttgaagatga gcagattgcc aattacattt
    11041 tggatttaga agttaaagta aaaaacggtg ctattattga tttcgtcagg tcagtgtcgc
    11101 ctattcttta ccgacttttt ctcagactaa tcacgtcaga aattccaaac ttcaaggctt
    11161 atatttttga tacaaagaat gaccaatatg atacctggca gtttcaggcc atgttggaat
    11221 ctgatcacga ggttttcaag gcttacctgt ctcaaaagca gtctcgcaat gtgacgacca
    11281 aaagcttagc agacatgttg acgttgacct ccttacctca ggaaatcaag gacttggttt
    11341 ttttgttacg acattttgaa aaggctgtcc gtaatcctct ggctcatttg attaagcctt
    11401 ttgatgaaga ggaactgcat cgcaccactc atttttcttc tcaggctttt ttggaaaaca
    11461 ttatcacctt agcgactttt tctggtgtaa tctaccgacg tgagcctttt tactttgatg
    11521 acatgaatgc cattattaaa aaggagttga gcctttggag acaatctatt gtctgatatt
    11581 tatcaaaaaa cacgataact tgaccgatac aggtcttact accgtgtttt tttagtatga
    11641 cgattgaaaa agacattaat caaaggttac ttcttcgaga aggtattcga taaaacgttc
    11701 ccccattttg gacaagttgg ctttttcatg cttgataaaa acaatgtcaa tttcgtcagg
    11761 gacatctagc gggatagcca caatctggtc accatttaga ttgctattta gaataccact
    11821 ggcaacggtg tagccatcta aaccaatcat taagttaaag agggtggcac ggtcactgac
    11881 cacaattgat ttgttgtggg gcatctggga catcatctct tctgaaaaat aaaaggagtt
    11941 gtgaatccct tggtcatagc tcaggtaagg aaaatcgctc aagtcgtcca tgcttaatag
    12001 gcttttagtt gctagaggat ttgatttgct gacaaaaata tgaggctgtg ccttgaataa
    12061 tggagaggca gtcaaatgat tgtcatcaaa taatttggtc aagacatctc ggttatagtc
    12121 gttgataaag agaacaccaa tttcagatcg aaagtttttg acatcatcaa tgatttccca
    12181 agttcttgtc tcgcgcaaaa agagttcata ccgagtcatg tcggtccgct tgaggagtga
    12241 gacaaatgca ttgactacga aggcataatg ttgagatgaa acgctaaaga gctctcggcc
    12301 agtattgtgg tttttatagc ggtcttctaa cagtgatgtt tgctctataa tttggcgagc
    12361 ataagataaa aattccaccc catcttttgt caaggtgatc cctttaggat ttcggttgaa
    12421 aatggtaatg cccatctcca tttccaaatc cttgaccgca ttggatagac ttggttgtgt
    12481 gataaataat tgcttagcag cttcattcat agagccgcac tcaacaattt tgataatata
    12541 atgcagttgt tgaattctca taagtacagt ctagctcaaa aaaaataaag acgcaagcac
    12601 aaacgtcatc atgggtttag aactttgaaa aagcgctgga gtcttttctc aatatggcct
    12661 aggataaata tgattcaatt ttgagagttg aatctatcaa tttcattgcc cttttatttt
    12721 tcttgtgtta tattaatagt attaacaaat aggtattttt ctaatcaaag ggtggtggtc
    12781 gtgtgaatgt tactgaacaa aatagtcatc aaatcttaat ccaaaaatta ttggtcagta
    12841 tccactattt gactcttttt cgagatgaac tgaagcttgt agaacgaact ccttctatac
    12901 ttggagggga atttccagct catttagtac agtctgagtt gggagatatt gttgcggcca
    12961 ttgatacctt ggatatgcag caacgcttga ttgagtctac cttttggtat gaagaatcag
    13021 cttttaagtt gatgaacaaa actttggata ttgtcgataa ttggattaaa ggagtggatc
    13081 atctgataga tttatgccag tccaaagaag tctttcaaat tattattgga gataaacgca
    13141 ttcgagtttt tggggtctta agtgatgttt tttcatcttt aaaagtgagt gctttgtcac
    13201 ttaaagaagc tcctattccg gatgtgctct atgaacatat ccagagggtt aatcttgaag
    13261 aagaagcttt tataaaatat taccaaagcc ctaagatacc cacaggtgag tttgaataaa
    13321 tgattgggta aaaatgataa aatgagttga cgtaagtata caaaaaaaga tatagtagga
    13381 aaaatgt
//
LOCUS       DAUCWU010000043        13198 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUCWU010000043 DAUCWU010000000
VERSION     DAUCWU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.043"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            45..962
                     /locus_tag="VGY24_001120"
     CDS             45..962
                     /locus_tag="VGY24_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284103.1"
                     /translation="MKSFVHPQREIFEAVKDLSFEVPKGQILGFIGANGAGKSTTIKM
                     LTGILKPTSGYCRINGKIPQDNRQDYVRDIGAVFGQRTQLWWDLALQETYVVLKEIYD
                     VPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLGQRMRADIAASLLHNPKVLFLDEPTI
                     GLDVSVKDNIRRAITQINQEEKTTILLTTHDLSDIEQLCDRIIMIDKGQEIFDGTVTQ
                     LKQSFGKMKSLSFELKPGQEQVVSQFMGLPDITVERHELSLDIQYDSSRYQTADIIQK
                     TMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            964..1782
                     /locus_tag="VGY24_001121"
                     /pseudo
     CDS             964..1782
                     /locus_tag="VGY24_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            1784..2569
                     /locus_tag="VGY24_001122"
     CDS             1784..2569
                     /locus_tag="VGY24_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284104.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            2770..2919
                     /locus_tag="VGY24_001123"
     CDS             2770..2919
                     /locus_tag="VGY24_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ4284105.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            3314..4462
                     /locus_tag="VGY24_001124"
     CDS             3314..4462
                     /locus_tag="VGY24_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ4284106.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADFILDLPGASDQGIRPRLSSKVLSKVPPILGEG
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKVRILGKAMPK"
     gene            4419..5666
                     /locus_tag="VGY24_001125"
     CDS             4419..5666
                     /locus_tag="VGY24_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ4284107.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCS
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            5722..6756
                     /locus_tag="VGY24_001126"
     CDS             5722..6756
                     /locus_tag="VGY24_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ4284108.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDFVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            6918..7628
                     /locus_tag="VGY24_001127"
     CDS             6918..7628
                     /locus_tag="VGY24_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4284109.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            7621..8973
                     /gene="vicK"
                     /locus_tag="VGY24_001128"
     CDS             7621..8973
                     /gene="vicK"
                     /locus_tag="VGY24_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ4284110.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADVELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTDTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            8977..9786
                     /locus_tag="VGY24_001129"
     CDS             8977..9786
                     /locus_tag="VGY24_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4284111.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            10228..10920
                     /locus_tag="VGY24_001130"
     CDS             10228..10920
                     /locus_tag="VGY24_001130"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ4284112.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            10921..>13198
                     /gene="smc"
                     /locus_tag="VGY24_001131"
     CDS             10921..>13198
                     /gene="smc"
                     /locus_tag="VGY24_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ4284113.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSD"
BASE COUNT         4107 a         2231 c         2782 g         4078 t
ORIGIN      
        1 aaaatttttc caaaaccatt aaagagccag gattaaaggg agccttgaag tcctttgttc
       61 atccccaaag agaaatcttt gaggcggtta aggacttatc ttttgaggta cccaaagggc
      121 aaattttagg ttttattgga gccaatggtg caggaaaatc aacaacgatt aagatgttaa
      181 caggtatttt aaagcctaca tcaggttact gtcgcattaa cggaaagatt ccacaagata
      241 atcgtcaaga ttatgtgaga gacattggag ctgtttttgg tcaacgaaca caactatggt
      301 gggatttggc cttgcaagag acctatgtcg ttttaaaaga aatttacgat gtgcctgaaa
      361 aagcctttcg caagcgcatg gattttttaa atgaggtttt ggacctgaat gagtttatta
      421 aggatcctgt ccggacctta tcactcggtc agcggatgcg agctgatatt gctgcttcct
      481 tgttgcataa tcctaaagta ttgtttttag atgaaccaac gattggactt gatgtttctg
      541 tgaaggacaa cattcggcgt gccattactc aaattaatca agaagaaaag acaactattt
      601 tactgacgac ccatgatttg agtgatattg aacagctctg tgatcggatt atcatgattg
      661 ataaagggca agaaatcttt gatggaacgg tgacccaatt aaagcaaagc tttggaaaaa
      721 tgaagagtct gtcttttgag ttgaagccag ggcaggagca ggtggtctca cagtttatgg
      781 gcttacctga catcacagtg gaacggcatg agcttagttt agacattcaa tatgacagtt
      841 cgcgctatca aacagcggac attattcaaa aaaccatggc tgattttgcg gtcagggatt
      901 tgaagatgac tgatgtagat attgaggata ttgttcgtcg tttctaccga aaggagcttt
      961 aagatgtggt cattttggaa acgttataga ccctttctca gtgcaggaat tcaggaatta
     1021 ataacctatc gcgttaattt ctttttgtat cgtattgaag atgtcatggg ggcttttgtg
     1081 gcttattacc tctggaaggc tgtttttgat tcgtctaagc aatccttaat caatggtttt
     1141 actctgtctg acatgacctt ttacattatc atgagttttg tgaccacttt actgaccaaa
     1201 tcagacagct cctttatgat tggcgaagaa gtgaaagatg gttctatcat catgcgcctg
     1261 cttcgaccag ttcattttgc agcctcttac ctctttatgg aaattggttt tcgctggatt
     1321 gtgctaatga gtgtcggctt tcctttttta atggtcttat ctggtattaa agtcatggcg
     1381 gggctgtcta tcttgtaagt cctagccagc agttgtctgt atttagttag ccttttatta
     1441 gcttttttaa tcaattttta tttcaacatt tgttttggct tttcagcatt tgtctttaaa
     1501 aacctttggg ggtctaattt attgaaaaat gccttggttg cctttatgtc aggcagtttg
     1561 attccattgg ctttttttcc taaaatggtt tctatagtgc ttagttttct accgttttcc
     1621 tctttggttt acacgccagt tatgattgtc attgggaaat acagccttag tcagataatg
     1681 gtggccctaa gtttgcaaat cttttggtta ctggtgatgg tagtcttatc gcaagtcatc
     1741 tggaaaaagg ttcagtacca tttaacgatt caaggagggt gagatggcaa aattgagacg
     1801 tatgcacgct atttttatca aacaatacct caaacaaatt atggaataca aggttgattt
     1861 tgtggtggga gtgctaggtg tttttctcac tcagggactt aatctgctct ttttatcggt
     1921 acttttccaa catatccctt ctttagaagg ctggacgttt gaacaaattg cctttattta
     1981 tggcttttct ttgattccaa agggaattga tcacctcttt tttgacaatc tttgggcttt
     2041 agggcaacgg cttgtccgca aaggagaatt tgacaagtac ctcactagac caattagtcc
     2101 tttgtttcat gttttggttg agacctttca agtggatgcc ctgggtgaat tattggttgg
     2161 ggttatctta ttagtgacca cggctggtag tatcgtatgg actcttccta aggtgctttt
     2221 gtttattttg gttattcctt ttgcgacctt gatttacacc tctttaaaaa ttgcgaccgc
     2281 cagcatttct ttttggacca agcagtctgg ggcagttatc tatatttttt acatgttcaa
     2341 tgacttttcc aagtacccta tgtccattta ccattccttt ttgcggtggt taatcagttt
     2401 tatcattccc tttgctttta cagcttatta ccctgcttct tattttttaa caggtcaaca
     2461 cctgctcttt aatattggag gcttggtagt ggtgtcgtta ctggttttgg ccttgtcgct
     2521 taagctttgg aaatggggcc tagatgctta tgaaagtgca ggttcctaga gagactctta
     2581 aacaaattga aaatcgttta ggtcagaata tgaaaaccta gataatgaag gaaggcagct
     2641 gttgtaaaaa cggctgcttt tttttgtgat ggtttaccaa gcgtaggcat tggttggaac
     2701 catcatcagc agattggtct aaattaaggg cggtaaaata acataagcag atatatttca
     2761 agtggttgca tgcaattgct agataacgtt tatggaactt tgagatcttt tgcctttatg
     2821 agacctgtca aatctagtaa cgataccagc ttagcagatg ccttgcaggc tgatacagac
     2881 atggctttta ttttagttag aggcattaac tatcaataaa tagtaaagac aaggtttgtc
     2941 aataccataa aaaagttaag tgtcacagct tagctttttt atggtgtcta attctttatt
     3001 ctatataaga ttttcttttc ttaggaatgg ttttccagta taataaagtc accctgtatc
     3061 aaaaacaaag gttacagttt gaatgttaaa gcaacaaagt aagtgacttc tacgacaata
     3121 gttgtgggac ttgacatttc aatgacgtta gagaatttta tagattttat tgatggcaca
     3181 ctagcgagac taaagagatt ctctaaatta atttaggtgt atttttagca gttattagtt
     3241 caaaaaagaa aaccatcaga aataattata taataaccat ttaaggacat cgaaaataaa
     3301 ggaggtcaat ataatgacgg atgtttatat tgctgcaggc cttagaaccc ctatcggttt
     3361 agtagggaaa caatttgcta aagaacaacc agaaatcctt ggagcaaagc tcatcaacgc
     3421 tttacaaaat aagtatccag ttcccattga ccaagtgatt tgtggcaata ccgtcggaac
     3481 gggtggtaat attgggcgct tgatgaccct gtattctcat ttaggagaat ctgtctctgc
     3541 tttgacggtt gatatgcagt gtgcatcagc tggtgcagct ctttccgtgg gttatgctaa
     3601 aatcaaggca ggtatggcaa gcaacctttt agtgggaggg attgaaagca gctccttaca
     3661 acctgaatcg gtatatgctt cagctgattg gcgccaagga gcctacaagg tagctcagtt
     3721 ttctccagat agtatcagcc cttttgcgat gattgaaggg gcagaacgag tggcaagaga
     3781 gcatggcttt acaaaggagt atttgaatca ctggacattg agaagtcatc aaaaggctag
     3841 ttattgccag gagcaagccc tgttagctga ttttattcta gacctgccag gggccagtga
     3901 tcaaggtatt cgaccgcgct tgtcaagtaa ggtgttgtca aaggtacccc ctatcttagg
     3961 agagggtcac gtgattagtg cagccaatgc ctgcctcacc catgatgctg ctgccttttt
     4021 acagctaagt agtcagcctt cagcttttaa actgattgat gtggtagaag tagcaggaga
     4081 cccacagcgt agccctttaa tggtgattaa ggctagtcaa gtccttttgg aaaaacatgg
     4141 tctagggatg gcagatatga cagcgattga atggaacgaa gcctttgccg ttattgatgg
     4201 tctatttgaa acccattacc cagatttatt ggaccgctat aatatatttg gtggtgcatt
     4261 agcttatggt catccctatg gtgcctcagc agccattata atattgcact taatgagggc
     4321 tttagagatt aaaaatggac gctatggaat agctgctata gcagcagctg gtggccaagg
     4381 gtttgctgtt ttactaaaat atcataagga gtttagtcat gctgacaaag ttagaatatt
     4441 gggcaaagca atgcccaaat aaaaaagcta tcgtagccga tcagataagc ttgacttatc
     4501 aagagctatg gcaagcggtt ttaataaagg atcagacaat aaaagacagt gtaccatata
     4561 tcatcagtca tagtcgctat cttaatcagt tactctcttt tttacgagga ttaaaagaag
     4621 gcagttgtcc tatcatttta caccctaata tctcaggtac atttcagcag caaataaaac
     4681 acgtcgatag tgagcttctt aaaaaagctg attttgctgt tttgagttcg ggaacaactg
     4741 gcaaagcaaa attattttgg cgttgtctat caacttggac gagacttttt gattatcaaa
     4801 ataaggtgtt tggtatgact ggtaatagtt gtctattctt acatggtagt tttagtttta
     4861 ctggcaatct taacttagca ttagctcaac tatgggccgg gggatgtctg gttttaagcc
     4921 aaaagctgtc tttgaaaacg tggttaagct tatggcaagc aaaaaaggtg agtcaccttt
     4981 accttttacc gacctaccta aatcgcttgc taccttattt gactaagaac aacatgactg
     5041 cgactcactt attgacctct tcgcaaatga tatcacaaga attactaaga cattattaca
     5101 aaaaatttcc tcagcttgaa attgtcattt tctacggggc tagtgaatta tcttttataa
     5161 cgtggtgtaa tggcagagct gctgtcaaaa ttaatggttt ggtaggacag ccctttcctg
     5221 atgtgtctat cagtttcaaa gataaggaaa tttttgtaga gactccttat agtgtagaag
     5281 gaatgtcaca accatacagt gtctcagact taggaaaaat gagcccagcg ggtttaattt
     5341 tagaagggcg acaagatgac tgggttaatc aacgaggagt gaaatgccac ctgcctagcc
     5401 tagttgaact cgctcaccaa gcgccaaacg tcaaagaagc gcacgcttta aagataggaa
     5461 aaggagaaaa cgaaacccta atattagtat tagttttgac taagaaggat tgcctagcac
     5521 ctattaaaga ctttttagct ctttatttaa actctggaca acttcccaag tactatcttg
     5581 tgattgattg ttcgccttta aaagataatg gtaaaattaa tcgagaagtt cttttaaata
     5641 aaatacccaa acagtggctt agttaaccac ttcaggtttc gttttgctta tagtgagata
     5701 caagtgacga ttcagcttta gatgtgttat aatatggtca aaaaagatga ggagaaggcc
     5761 atgaaacaag ctatcttaga tcgctatcag gccttgaaat gctatcaaaa cgcaggttta
     5821 tcaaaccaag catttcgtgc tatagctaaa gagcctatca ttgataatcg acttggttct
     5881 ccaactttct gggtaatatg gcctattgaa aaagaaaacc aatcagctaa gcagttacta
     5941 acatttcttc ttgattttgt agagatgcca tttgagctaa gtggtcagtt gcatgaaact
     6001 caaacgctac tcacccgatt ccatccatct ctattgccag atcatatgtt ttggaaagag
     6061 ttggctagct tagttgatca agcttttcct ggcaaaacac taagccaagc aggtgagctt
     6121 gaaaagcgtt tgcatcaatt tcgttatgtc atctctagtc aacaagctca atctatcaga
     6181 aaccattata aaatgattga gatgacagac gcccaagctc tggccctttt tttgaggtct
     6241 aaaaaagggc cttgtttgtg gcggcaagca ccagattata ccttgatgga ttctgccagg
     6301 ttacataata aactaagatt tgaagataat aaagtcattt ttccaagtca agaagtttcc
     6361 tataatataa aagtacttct ttggtttcat accgaattta ttctagatag cacaggcttt
     6421 tttcttaatg aagttgatgc agaagtagtg accgaaaaag gaattgtcaa tggtgcatct
     6481 tttaactatg gcacagatgg tcctagacat tgggatttag atgttgatcc gattagccgt
     6541 cacgatcctc agtttagaag ggacaccctt aaaggatttc gatcgccaaa aagagtattt
     6601 cgtcagtggt ttagagctca aaaagatgac tttatgttct cttattttaa tgccaaagga
     6661 ctttttgctt accacaacaa gagtagtttt gccagagtca aaaaatctgc taagcaattc
     6721 aaacgtcaaa ttcatcctat aaagggttgg ttttagaaag tactctattt gtaacggtag
     6781 tcgtcattgt caattaggct gaatttttgg tataatcagg tagaaataat ttagaaaaat
     6841 atagttttgt gattttttat taaccacaaa aatcacaaat tgtaatatca attacaagaa
     6901 ctttctgagg tatagacatg aaaaaaatac ttattgtgga tgatgaaaaa ccgatttctg
     6961 acattattaa gtttaatttg acaaaagaag gttatgacat tgttacagct tttgatggac
     7021 gcgaagcggt aacaattttt gaagaagaaa agccagattt aattattctt gatttgatgc
     7081 tccctgagtt ggacggtctt gaagtagcca aggaaattcg taaaaccagt catgtcccga
     7141 ttattatgtt gtcggctaaa gatagtgagt ttgacaaggt tattggactt gaaattgggg
     7201 ctgatgatta cgtgaccaag cccttttcta atcgggaatt gctggcacgt gtcaaggctc
     7261 atctgcgtcg taccgaaact attgaaacgg ctgttgcaga agaaaatgct tcttcaggta
     7321 cacaggaact aaccattggt aatttacaga ttttaccaga tgcgtttgtt gctaaaaaac
     7381 atggtcaaga ggtagagttg acccatcgtg aatttgaact attgcatcat ctagctaacc
     7441 atatggggca ggtgatgaca cgagaacact tattggaaat tgtttgggga tatgattatt
     7501 ttggcgatgt gcgcacggtt gatgtgactg ttcgtcgtct ccgtgaaaaa attgaagaca
     7561 caccaagtcg tcctgagtat attttaacaa gacgtggtgt tgggtactac atgaaatctt
     7621 atgactagag atatcattgg aaacttatcc acatttgaat tagcgatcct tatcttactt
     7681 gtttttgttg ctttttactt tatccatctt gcggtgcgtg attaccgaaa tgcacgtatt
     7741 attcggatga tgagccataa aatccgagac ttgattaatg gtcgctatac tgatataatc
     7801 gacgaaaaag cagacgttga gttaatggag ctttcagacc agttaaatga cctgtcagat
     7861 gtttttcgct tgacgcatga aaatcttgcc caagaaaaaa atcgcttggc aagtattttg
     7921 gcttatatgt cagatggtgt acttgctaca gaccggtctg gtaaaatcat catgattaac
     7981 gagacagctc gcaagcaatt aaatttaagt aaagaagagg cactaaagaa aaacattaca
     8041 gatttgttag aaggtgatac ttcatatacc taccgtgatt tggtatccaa aacaccagtg
     8101 gtaactgtta atagccgaaa tgatatgggt gagtttgtct cattacgctt gcgctttgcg
     8161 ttgaatagga gagagagtgg ttttatttcg ggcttggttg tggtgctcca tgacaccaca
     8221 gaacaagaaa aagaagaacg tgaacgccgt ctttttgtct ctaatgtaag tcatgaatta
     8281 aggacccctt taacttcggt taaatcctac ttggaggctc ttgatgaagg tgcacttaaa
     8341 gaagatattg ctccaagttt cataaaagtt tctcttgatg aaactaatcg gatgatgcgt
     8401 atgatttcag atcttttaaa cctgtctcgg attgataatc aagtaaccca attagcagta
     8461 gagatgacta attttactgc ttttataact tctattttaa acagatttga tttggttaaa
     8521 aatcagcata cagatacagg aaaagtctat gaaattgtaa gagattaccc tattacctct
     8581 gtctggcttg aaattgataa tgataaaatg acacaggtta tcgagaatat tttgaacaat
     8641 gccattaagt attctccaga tggtggaaaa atcacagtcc gtatgaaaac aacagatacc
     8701 caattaatta tttccatttc agaccaagga ctaggtatcc ctaaaacaga tttgcctctt
     8761 atttttgatc ggttctatcg tgtagacaag gcaagaagtc gtgcccaagg agggaccggt
     8821 ctaggccttg ccattgctaa agaaatcatc aagcagcacc atggctttat ctgggctaag
     8881 agtgactatg gtaaaggatc gacctttact attgtcttgc cttatgaaaa agatgcagcc
     8941 atctatgaag aatgggagga agatgtagac taatcaatga atgagagtgg ttttaaatat
     9001 agtattttag catctgggtc cacaggaaat tgtttttatt tagaaacacc taaaaaacgc
     9061 ttattgattg atgcagggct tactggtaag aaaattacca gtcttcttgc tgaaattgac
     9121 cgcaaacctg aggatttgga tgctattttg attacccatg agcactctga ccatattaag
     9181 ggtgtaggcg ttatggcccg caaataccat ttggatatct atgctaatga aaaaacatgg
     9241 cagctgatgg atgagtgcaa tatgctaggt aagcttgatg cctcacaaaa acatattttc
     9301 caaagggata aagttttgac ctttggagat gttgatatcg aaagttttgg agtcagccat
     9361 gacgctattg atcctcaatt ttatcggatt atgaaagata ataagtcttt tgtgatgttg
     9421 acagatacag gatatgtcag tgataggatg acaggtatta ttgaaaatgc agatggctac
     9481 ttgattgaat ccaatcatga tattgaaatt ttgagatcag gttcttatcc ttggagcttg
     9541 aagcagcgta ttttatctga tttaggacat ttatcaaatg aagatggagc gggagccatg
     9601 attagaagcc tggggtacaa taccaaaaaa atatacctag gtcatctgag taaagaaaat
     9661 aacatcaaag agttagcgca tatgacgatg gtcaatcaac tggctatggc agatttagca
     9721 gtaggtacag actttacggt ccatgatacc tctccagata ctgcttgtcc attaactgat
     9781 atttgagata agaaactaaa agagaatatg caaagtagcg ctgattggtg atataccagt
     9841 cagggctatt ttttgatgtc ttaaagcgta ttgtcttctg agttcaatga actctcattg
     9901 gtcatccttt agagcatcaa tagacttaaa ttagcctgta gttttggctt gtctgatcta
     9961 tttatcaaag gtgttgagga ttataaagat gaggtagttt ttagttgttc agactaaaaa
    10021 caaaatagct ctatactatc tgtagtggga ctgtagtggg aaattcccct cagatagtgt
    10081 agagtctttt gattgtatga gaaagtttcc tttgacgggt atttaagatg tttaataaat
    10141 cacgaaaaat ccacttagag tcggttttct aggtggattt ttggtatact aaataaggtc
    10201 tactgacaaa tatgaaaggg attttttatg aaacagcttg aagagttact ctcaacatct
    10261 tttgatattc aatttaatga tttgacttta ctagaaacag cttttaccca cacctcttac
    10321 gctaacgagc atcgtctcct aaacgtttca cataatgaac gcttggaatt tttaggagac
    10381 gctgttctac aattgattat ttcagagtat ttatttgcaa agtatcctaa aaagacagaa
    10441 ggagatatgt ctaagttacg ttccatgatt gtacgtgagg aaagcttggc aggtttttca
    10501 cgattttgtt cttttgacgc ttatatcaaa ctaggaaaag gtgaggaaaa gtcaggtggt
    10561 cgccgtcgtg atactatttt aggagactta tttgaagcct ttttaggtgc tcttttatta
    10621 gataagggga ttgatgcagt acgtcgtttc ttgaaacaag tcatgattcc tcaggtggaa
    10681 aaagggaatt ttgaaagggt taaagactat aaaacttgtc ttcaagagtt tttgcaaaca
    10741 aaaggtgatg tggctattga ttatcaagtc attagtgaga aaggacctgc tcacgctaaa
    10801 caatttgagg tatcgatagt tgttaatggt gctgtcctta gtaaaggttt aggtaagtct
    10861 aaaaaattag cggagcaaga tgctgctaaa aatgcccttg cccaacttag tgaggtttag
    10921 atgtttttaa aagaaattga gttggaaggg ttcaagtcct ttgcggacaa gactaaaatt
    10981 gaatttgata agggagtaac tgcagtcgta ggaccaaatg gttcaggtaa aagtaatatt
    11041 acagaaagtt tacgttgggc tttaggagaa tcaagtgcta aaaatctgcg tgggggcaag
    11101 atgccagatg tcatttttgc tgggacacaa aaccgtaatc ctctaaacta tgctaaggta
    11161 gcggttgttc ttgataattc cgatcatttt ataaaaactg ccaagaaaga aatccgtgtc
    11221 gaaaggcata tttaccgaaa tggagatagt gactatctga ttgatggtcg caaggtaagg
    11281 ctccgtgata ttcatgattt gtttatggat acagggcttg gtcgtgattc tttctccatt
    11341 atttctcaag gtcgtgtaga ggaaattttc aatagtaaac ctgaagaaag gcgcgccatt
    11401 tttgaagaag cagctggtgt tttaaaatat aaaacacgta aaaaagaaac tcagattaaa
    11461 ttaaatcaaa ctcaagataa tttagaccgt ctagaggata ttatttatga gctagacacg
    11521 cagttggccc cacttgaaaa gcaagctaaa gtagctaaac aatttttaga acttgatgcc
    11581 aatcgtaagc aactacaact agatattcta gtcaaagata ttgatatagc tcaagaaaga
    11641 cagactaaag atactgaagc tttagctgct ttacaacaag acttagcatc ttattacgct
    11701 aagcgtcaat ctatggaaga agattaccaa aaattcaagc aaaaaaagca ggttcttagt
    11761 caagagtcag atcaaaccca aacaacatta ttagaattaa ccaagctaat agctgacttg
    11821 gaaaagcaaa ttgaattggt taagctagaa agtggccaag aagcagagaa aaaggcagaa
    11881 gccaaaaaac acctcgaaca gttacaagaa caattggatg gttttcaagc agaagaaaag
    11941 cagcgtacag aacaattatt gcatattgat caacaacttt gtgatgtcaa acaacagtta
    12001 aacgaattga gcaatgcatt agagcgtttt tcaagtgatc ccgaccaatt aatggaaact
    12061 ctaagagaag agtttgtatt attgatgcaa aaagaggcgg ccttatcaaa tcagctgaca
    12121 gctttaaaag cccatttaga caaagaaaaa caggctcgtc aacataaggc tcaagaatat
    12181 cagctacttg ttactaagct tgatcaactt aatgacgagt ctcaaaaagc tcaagctcat
    12241 tataaagctc aaaaagagca agtcgaaatg ttgctccaaa attatcaaga aggtgataaa
    12301 cgcgttcagg aattagaaag agattaccaa ctgaatcaag aaagactatt tgacttgttg
    12361 gatcaaaaaa aaggaaaaga agctcgtaag gctagcttag aatctattca aaaaagtcat
    12421 agtcaattct atgcgggagt acgagctgtc cttcaatcac aaaaaaagct tggagggatc
    12481 ataggtgcag tcagtgaaca cctttctttt gattctgatt accaaacagc tcttgaaatt
    12541 gcattaggtg ctaatagtca gcatattatt gtaacagacg aagctgcagc aaaaagagca
    12601 attgcttatt tgaaaaaaaa tcgccaagga cgagcaactt ttttaccttt aactactata
    12661 aaggctagat cattgtcaga acactatcat cgtcaattag cgacttgtga aggttatctg
    12721 ggaactgcag aatcattggt tcgctatgat gatagtcttt cagctattat ccaaaacctt
    12781 ttatctagta ctgctatctt tgaaactatt gatcaagcta atattgcagc gcgcttatta
    12841 ggatataagg taagaattgt tactcttgat ggaacagaat tacgaccagg tggttctttt
    12901 tcaggaggaa ctaatagaca aagcaataca acttttatca aaccagagtt agaacaaatc
    12961 agtgaggaat taacgcgatt agttgagcaa ttaaaaataa ctgaaaaaga agtagctgcc
    13021 ttgcaaagcg atttgatagc aaaaaaagaa gaactgacac aattgaaatt agccggtgac
    13081 caggcacgct tagctgaaca aagagctcaa atggcttatc agcaattgca agaaaaacaa
    13141 gaagattcaa aggctttgtt agctgccctt gatcaaagtc agacgactca ttctgatg
//
LOCUS       DAUCWU010000044        13169 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUCWU010000044 DAUCWU010000000
VERSION     DAUCWU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.044"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1298
                     /gene="glnA"
                     /locus_tag="VGY24_001132"
     CDS             <1..1298
                     /gene="glnA"
                     /locus_tag="VGY24_001132"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ4284114.1"
                     /translation="TFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEGF
                     VRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKH
                     MNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDLAPIDLADNTRREIVNIL
                     TKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAK
                     PKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAYYFLGGLMKHAYNYTAIT
                     NPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLELRSVDPTANPYLAL
                     AVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNALKALQKDDVV
                     QKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(1526..3208)
                     /locus_tag="VGY24_001133"
     CDS             complement(1526..3208)
                     /locus_tag="VGY24_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ4284115.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(3210..3440)
                     /locus_tag="VGY24_001134"
     CDS             complement(3210..3440)
                     /locus_tag="VGY24_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ4284116.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLNYEKETGVFELTEF"
     gene            3724..4422
                     /gene="tsaB"
                     /locus_tag="VGY24_001135"
     CDS             3724..4422
                     /gene="tsaB"
                     /locus_tag="VGY24_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ4284117.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHASLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            4394..4849
                     /gene="rimI"
                     /locus_tag="VGY24_001136"
     CDS             4394..4849
                     /gene="rimI"
                     /locus_tag="VGY24_001136"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ4284118.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQELGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
     gene            4839..5867
                     /gene="tsaD"
                     /locus_tag="VGY24_001137"
     CDS             4839..5867
                     /gene="tsaD"
                     /locus_tag="VGY24_001137"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ4284119.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(6038..6307)
                     /gene="rpsN"
                     /locus_tag="VGY24_001138"
     CDS             complement(6038..6307)
                     /gene="rpsN"
                     /locus_tag="VGY24_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ4284120.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(6543..7286)
                     /locus_tag="VGY24_001139"
     CDS             complement(6543..7286)
                     /locus_tag="VGY24_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4284121.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSC"
     gene            7526..8305
                     /gene="udp"
                     /locus_tag="VGY24_001140"
     CDS             7526..8305
                     /gene="udp"
                     /locus_tag="VGY24_001140"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ4284122.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            8326..9528
                     /locus_tag="VGY24_001141"
     CDS             8326..9528
                     /locus_tag="VGY24_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ4284123.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKGVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDYKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            9558..10229
                     /gene="deoC"
                     /locus_tag="VGY24_001142"
     CDS             9558..10229
                     /gene="deoC"
                     /locus_tag="VGY24_001142"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ4284124.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(10324..11493)
                     /locus_tag="VGY24_001143"
     CDS             complement(10324..11493)
                     /locus_tag="VGY24_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ4284125.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEENHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSADQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            12164..>13169
                     /locus_tag="VGY24_001144"
     CDS             12164..>13169
                     /locus_tag="VGY24_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284126.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKPAPITGMYYTL
                     GKIDSSSLKVLTASGLKLIYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQISQLSPWNKVRNWKRLELLGIENGSINNGEINEPESQYIQI
                     A"
BASE COUNT         4050 a         2203 c         2698 g         4218 t
ORIGIN      
        1 taacgtttct tcgcttgatg ttcactgata tcatgggcgt tatgaaaaat gtggagattc
       61 ctgcaacgaa agaacagtta gacaaagtat tgtctaacaa ggttatgttt gatggttcat
      121 ctatcgaagg ttttgtacgg atcaatgagt cagatatgta cctttacccc gatttagaca
      181 cttggattgt ttttccctgg ggagatgaaa atggagcagt tgcaggttta atttgtgata
      241 tttatacagc agaaggaaag ccttttgcag gagatcctag aggaaattta aaaagagccc
      301 tgaaacacat gaacgagatc ggctacaaat catttaatct tgggccagaa ccagaatttt
      361 tcctttttaa gatggatgat aaaggtaatc cgacacttga agttaacgat aatggtggtt
      421 attttgattt agcgccaatt gacttagcag acaacacgcg ccgtgaaatt gtgaatattt
      481 taacgaaaat gggttttgaa gtggaagcta gtcatcatga agtggctgtt ggtcaacatg
      541 agattgattt taaatatgca gatgttttga aagcttgtga taatattcaa atttttaagc
      601 tagttgtaaa aacgattgcc cgtgaacatg gactttatgc tactttcatg gctaaaccaa
      661 aatttggaat agctggatca gggatgcact gtaacatgtc tttgtttgat aaccaaggta
      721 ataatgcttt ttatgatgaa actgataagc gaggaatgca gctatcagaa gatgcttatt
      781 atttcttggg aggactaatg aagcatgctt ataactacac tgctatcact aaccctacag
      841 tgaattctta caaacgatta gttccgggtt atgaggcacc tgtttatgtc gcttgggctg
      901 gaagtaatcg ttcaccgctt atccgtgttc cagcatcacg tggtatggga acgcgtttgg
      961 agttacgttc ggttgatccg acagctaatc cttatttagc cttggctgtt ctcttggaag
     1021 ctggattaga tggtatcatt aacaaaattg aagctccaga acccgttgaa gctaacattt
     1081 ataccatgac aatggaagaa cgaaatgaag caggcattat tgatttgcca tcaacgcttc
     1141 ataatgcctt aaaagctctt caaaaagatg atgtggtaca aaaggcacta ggttaccata
     1201 tctacactaa tttcttagaa gcaaaacgaa ttgaatggtc ttcctatgca acttttgttt
     1261 ctcaatggga aattgaccat tatattcata actattagac ttatcaggac ctgctcaagt
     1321 tgagtgggtc ttttggatga gctattgtat ttgagctagc aagctattat tagctgatcg
     1381 gacaccaatt gattttccat tatagaaaac taaaaaccag tccatgttct atgaggactg
     1441 gtttttagtt tgataattat ctgaactaga actgatatgt taaagatata gacagatagt
     1501 attaagaaat gagtcttatt tgattttagt gcttatcagg tgttaagacc atcggaatta
     1561 taattggttc acgctcagtc ttttcataca agaatggacg aagagcattg acgatagcac
     1621 cgttaactga ttggatgcta gcatccttat tttttaaagc aatacggata gcgttaaaga
     1681 gcacacgttg gctttcgcgg atcaagtcac ctgactcacg catataaatg aaaccacggc
     1741 tcagaatatc tggtccagct aaaatcattt gtgtgttaaa atcaacagtt gcaacagcta
     1801 agacaacacc atcttctgac aaatcacggc ggtcccgtaa aacggctgct ccaatatccc
     1861 cgatgccatt accatccacg tagatgtctt gggcattaaa gtgaccagca atacgcgcac
     1921 tgtcactggt taaagctaga acgtctccat tttccataat aaagatattt tcttttggaa
     1981 taccaatatc catcgccagt cctgcatgaa ctttttgcat gcgatactca ccatgtactg
     2041 gcatgaaata ttttggttta attaaactaa gcatgagctt ttgttcttgc tgtcctccat
     2101 gaccagaagt atgaatatta ttgacttttc catgaataac atcaacacca gcttcttgga
     2161 tagtattgat taatttattg acacttgtgg tattaccagg gattggactt gatgagaaaa
     2221 tcacagtatc accaggctgt aaagttactt gacgatgagt gccattagcg atacgagcaa
     2281 gtgctgccat agactcacct tgactacctg tacacataat taaaacttca ctagcatgaa
     2341 gatttttaag ttcgcttggc tcaatgaagg taccttttgg aactttaata tagccaagtt
     2401 caataccgtt aacaattgcc ttttccatag aacgaccaaa aacggcaatt ttacgtcccg
     2461 ttttaacagc agcttctgca gcctgttgca aacggtagat gtttgaagcg aaggaagcaa
     2521 aaataatacg accatgaata ccttcgataa tttttaaaat ggattggcca acaacttttt
     2581 cagagttagt aaaggttggg atttcagcat tagttgagtc tgataataag caaaggacac
     2641 cttcttcacc aagcgctgcc atacgttgta aatctgcagg atctcctact ggcgtgaagt
     2701 caaatttaaa atcaccagta cagataatct taccttgagg ggtgtggata acaataccga
     2761 ccggttctgg aatggaatga gtagtcttga agaaagtcac gctcatgttt ttaaaagtta
     2821 actcggtgtt gtgattgatt tcgtaaacag ttgcttcacg ccaaagacca tgttcttcca
     2881 atttaccacg gataagagca agtgctaaag gtcctgcgta gatagggata ttagcctgtt
     2941 tgagaagaaa tggaatacca ccaatgtggt cttcgtgacc atgtgtaata acaagagctt
     3001 tgacccggtc taagttatcg acaatgtaag aatagtctgg aataacgtaa tcaattccca
     3061 aaagatcatc ttctgggaat ttgataccgg catcaacgat aatgatctcg tcttgatact
     3121 caataccata agtattcttc ccaatctctc ccagaccacc aatggcaaac accccaactt
     3181 cattaggttt taaactgata tttgtcatct tagaactccg ttaattcaaa aacaccggtc
     3241 tctttttcgt aattgaggtg tttgtcagaa agtagctcga taaattccac gttataatag
     3301 gtgttatctt caacgagacg acgagccttg atacggccat cgagttcatc agtagcatca
     3361 atattaagat agagtgcttt agtactttca cgacgtgggc tttgatcctt tgtttcttga
     3421 tagaaaactt tataaatcat ttgtttttaa ttcctttcgt tatgaatcaa ttatccaaac
     3481 aataataaat aggtcaaaag gtaggtgttc cattttgacg gttttatatg ttgtcattat
     3541 tgtgtagtag attaattcct gattctgtgc aattattata attataccat aaacttagct
     3601 tttagtaaaa aatttaggta catttatggt atagtctaaa tcaaattaag agtcagcctt
     3661 tttgtttatg attcttaatt tgtgttataa ttgatactag aacttgcagt agaaaggcat
     3721 aaaatgaaga cacttgcatt tgatacctca aataaaacct tgtcccttgc tatacttgat
     3781 gatgagacac ttctagcaga tatgaccctt aacattcaga aaaatcatag tgttagcctt
     3841 atgcctgcta ttgatttttt gatgacttgt actgatctta aacctcaaga tttagaaaga
     3901 atagtggttg caaaaggccc tggatcttac acaggtttac gagtggcagt tgctactgca
     3961 aaaacgttag cgtacagttt aaatattgca ttggtcggga tttccagtct atatgctttg
     4021 gctgcgtcta cttgtaaaca gtatccaaat actttggtgg tgccattgat tgatgctaga
     4081 aggcaaaatg cgtatgtagg ttattatcgg caaggaaaat cagtgatgcc acaagcccat
     4141 gcttcactag aagttattat agaacaatta gtagaagaag gacagctgat ttttgttggg
     4201 gagactgctc cttttgctga gcaaattcaa aagaaactac ctcaggcgat actacttcca
     4261 acccttcctt ctgcttacga atgtggtctt ttggggcaaa gtttggcacc agaaaatgta
     4321 gacgcctttg tccctcaata tctcaagaga gtggaagctg aagaaaactg gctcaaagat
     4381 aatgagataa aagatgatag tcactacgtt aagcgaatct aatatgaaaa cagttgaaga
     4441 acaagcaaaa aatatttatc agcttttgga aatggtttat ggcacgtctc cttggacttt
     4501 agaacaagta ttaatcgata tacggcggga tcaaacagat tatttcctac tatatgatca
     4561 tgataaattg ctaggttttt tagctattca agatttagct ggtgaagttg aaatgactca
     4621 aattgctata ctacctagtc atcaagaact tgggctagcg tctcaattga tgacgcattt
     4681 agatagcatt gaaagtgaca tctttttaga ggttagagaa tctaatcata gagcacaggg
     4741 tttatatcag aagtttggtt ttaaatttat tggcaaaaga ccagactact atagaaatcc
     4801 tatagaaaca gctttattga tgaaacggga aggaaaacat gacagatagg tatattttag
     4861 cagttgagag ttcttgtgat gagaccagtg tagcgatttt aaaaaacgaa agtaccctct
     4921 taagtaatgt cattgcaagt caggttgaaa gccacaaacg ctttggtggt gtggtgccag
     4981 aagtagctag tagacaccat gtcgaagtga ttacgacttg ctttgaggat gctttgcaag
     5041 aagctggcat cagtgcttca gatttatcag cagttgcggt aacttatggc cctggacttg
     5101 ttggagcctt gttagttgga ttagcagctg cgaaggcttt tgcttgggca aatcatttac
     5161 ctttgattcc ggttaatcat atggcaggtc acttaatggc agcgcgtgaa caaaagcctt
     5221 tagtttatcc tttaatagca ctattggttt caggaggaca cacagaattg gtttatgtcc
     5281 cagagccagg ggattatcat attattggcg agacgcgtga cgacgctgtt ggtgaggctt
     5341 atgataaagt tgggcgtgtg atgggcttaa cctatccggc aggacgtgaa attgaccagt
     5401 tggctcataa aggtcaagat acttatcatt tccctcgtgc tatgataaca gaagaccact
     5461 tagaattttc tttttcagga ctcaagtccg cttttatcaa tcttcatcat aatgctaagc
     5521 aaaaaggcga cgaactgatt ctagaagatt tgtgtgcatc ttttcaagca gctgttctag
     5581 atattttatt ggccaaaaca aaaaaagctc tgagcagata tcctgcaaag atgcttgtag
     5641 tggctggtgg tgtagcggca aatcaaggat tacgagatag attagcacaa gaaattacac
     5701 atatagaagt tgtgattcct aaactaagac tctgtggaga taacgcgggt atgattgctt
     5761 tagcagcagc tattgagtat gacaaacagc attttgctaa tatgagccta aacgcgaagc
     5821 ctagtttagc ttttgaccag tttccagaca gttttgtaat aaactagtca cacttgctag
     5881 ttagtaagat ttataatgga taagaatctc cctttgaaaa aacaacccgg tatcttggca
     5941 taccgggttg tcatgatgat aagtaattgg cttctatggt tactaataat tcagataatt
     6001 acctgcaaaa taattgtctg cctaaggtga agtaagctta ccagctagct ttagtgacgc
     6061 caggaagctg tcctttgtga gctaagtcac ggaaattgat acggctaaca ccaaacttac
     6121 gcatgtaagc atgaggacgg ccgtcaattt tgtcgcggtt cttcaagcgg tttggatttg
     6181 aatcacgagg caatttgcgc agtgattcat aatcgccctt cgcttttaag tcacgacgaa
     6241 ggtcagcgta ctgttcaatc agttggagct gcttttggta tttagcaatt tttgactttt
     6301 ttgccattgc tttctccttc ttaatattta ataacctcag agtcgttatc agcgctctga
     6361 atccacttaa ctggtattta actggttaag ttgatatata atcattatac aataaataaa
     6421 agcaaaagca aataaaaaaa attaactagt tatctttttt agccataata tcaaaaaact
     6481 ccacatctaa gtgtgggagt caggtattaa taactgttta aaggtgttta atgagtgtcg
     6541 ctttagcaac tttgagattg aacttttaag tcaaataaat cagcaggaac gtagtgtttg
     6601 gttgagacca agatgtcttt accattgtag agagtttctt ggattaaaat aaaagcacta
     6661 ttttctgata gagtatactt agttgctgtg taattgccag tcttggtata gccgatttgg
     6721 ttgcaggaat gagaagcttt accagattca tagattttct cttctaagaa agtaagaagg
     6781 ggctcttctt gattgagatt tatagcatat ttagaaataa cctcaatagg gataaaagat
     6841 aaactataag caatagcttt attttggcta tgataccagc gatcaacaat aatcacaaca
     6901 ggagtttctt gttttaggga tgctgtaatg gcaacagtgg gcacttcaat ccgatattcc
     6961 aactcaacct ttgtgatgtc agatgatagg cttgcatata tggggtgttg aagatattcg
     7021 aaaccttggt gatatttagt ttcaggagtc ttgagtataa aatttccctt tcctctgata
     7081 ttttttatga gatgatcctc ttggaggagg gctagggatt tacgcagtgt catccgactg
     7141 acattgagct gtctagcaag ttcaggttct gatggcagtt gcatgccagg actatatgta
     7201 ccatcttgaa taagttgaaa aatagtatta tatacttgca catgtttaag ttttttgagt
     7261 tttttggtta agtcgttagt agacatatca cttacctcat taataatctt tagtggagtt
     7321 taacttgtct aaaaactatt ttagcactaa cagtattcct tgattagaat tgtacccatc
     7381 tatagtgtta gtatctcaca ctcaggtact ttttgtcaaa aaagagatga aaaacacctt
     7441 aaaaaagcgt ttacaaaata gagtgtgtgt gatactatac aagtgtatca aaaattagac
     7501 aagtaaaata caggaggttt ttaacatgca aaattattca ggtgaagtcg gattgcaata
     7561 tcacctacag attcgtccag gtgatgtagg tcgttatgtt atcatgccag gtgatccaaa
     7621 acgttgtgca aaaattgcag aacattttga taatgcagtt cttgtagcag acagccgtga
     7681 atatgttacg tatacaggta cattaaacgg tgaaaaagtt agtgttactt caactggtat
     7741 tggtggtcca tcagcgtcta ttgcgatgga agaattgaaa ctttgtggcg ctgatacctt
     7801 tatccgtgtt ggaacatgtg gtggtattga ccttgatgtt aaaggcggag acatcgttat
     7861 tgcaactgga gctatccgta tggaaggtac cagtaaagaa tatgctccga ttgaatttcc
     7921 tgctgtagct gacttggaag tgacaaatgc tctagttaat gcagctaaaa agcttggtta
     7981 tacaagtcac gcaggagtag ttcaatgtaa agatgcattt tacggacaac atgagccaga
     8041 acgtatgcca gttagttatg aattattaaa taaatgggaa gcatggaaac gtctgggaac
     8101 aaaagcgtct gaaatggaat ctgcagctct ttttgtagca gcaagtcatc taggagttcg
     8161 ttgtggatca gatttccttg ttgttggtaa tcaagaacgc aacgctttgg gtatggataa
     8221 cccaatggct catgacacag aagcagctat tcaagttgct gttgaagcac tccgcacact
     8281 tattgaaaat gataaatcac aataagcgag gttttggaag tcatcatgca atttatttat
     8341 agtattattg gtattttatt ggtattagga attgtgtatg caatttcttt caatcgtaag
     8401 ggtgtttctc taagtttaat tggaaaagct cttatcgttc aattcattat tgcgctaatc
     8461 ttagtacgta tcccactagg ccaacaaatt gttagtgttg tttcaactgg agttactagc
     8521 gtaatcaact gtggtcaagc tggtttaaat tttgtgtttg ggtcattagc agatagtggc
     8581 gcaaaaactg gttttatttt cgctattcaa acgcttggta atattgtttt cttatctgcc
     8641 ctagttagtc tactttatta tgtaggaatc cttggatttg tagtaaaatg gataggtaag
     8701 ggcgttggta aaattatgaa atcctcagag gttgagagtt ttgttgctgt agctaatatg
     8761 tttcttggtc aaacagacag tccaatcttg gttagcaaat acctaggtcg tatgactgat
     8821 agtgagataa tggttgtgtt ggtatcaggt atgggaagta tgtcagtttc tattcttggt
     8881 ggctatattg cattaggcat tccaatggaa tatctcttga ttgcttcaac aatggttcct
     8941 attggcagta ttcttattgc taaaatctta ttgcctcaaa cagaacctgt tcaaaaaatt
     9001 gatgacatta agatggataa taaaggtaat aacgccaatg tgattgatgc aatcgctgag
     9061 ggtgcaagca caggtgcaca aatggctttc tcaattggtg ctagtttgat tgcctttgtt
     9121 ggtttagttt ctttgattaa tatgatgtta agtggattgg gaatccgctt agaacaaatc
     9181 ttttcatatg tttttgctcc atttggtttt cttatgggat ttgactacaa aaacattctt
     9241 ctagaaggaa accttcttgg aagtaagttg attttaaatg agtttgtttc gttccaacaa
     9301 ttgggtcacc taatcaaatc tttagattat cgtacagcat tggtagcaac tatttcactc
     9361 tgtggttttg ctaatttatc aagtttaggt atttgtgttt caggtattgc tgttctttgc
     9421 ccggagaaac gtagcaccct agctcgactt gttttccgtg caatgattgg tggtattgct
     9481 gtaagtatgc ttagcgcctt tatcgtcggt attgtaactc tattctaagc gttgacaaaa
     9541 gaaagaagga tagtaacgtg gaagtaaaag atattttaaa aacggtagac catactttgc
     9601 tagcaacaac agcaacgtgg ccagaaatcc aaacaatttt agatgatgcc atggcttatg
     9661 aaacagcttc agcatgtatt ccagcttctt acgtcaaaaa agcagcagaa tacgtttcag
     9721 gtaaattagc tatttgtact gttattgggt tcccaaatgg ctatagtaca actgcggcga
     9781 aggtttttga atgtcaagat gctattcaaa atggtgctga tgaaattgac atggtcatta
     9841 atttgacaga cgttaaaaat ggggattttg atactgttga agaagaaatt cgtcaaatca
     9901 aagctaaatg tcaagaccat atcttaaaag ttatcgttga gacatgtcaa ttaactaaag
     9961 aagaacttat cgaactttgt ggagttgtca cacgttcagg tgcagacttt attaaaacct
    10021 ctactggttt ttcgacagca ggtgctacat ttgaagatgt tgaagtgatg gcaaaatatg
    10081 tcggcgaagg tgttaaaatt aaggcagcag gtggaatctc atcattggaa gatgctaaaa
    10141 catttattgc tttaggagct tcacgcttgg gtactagccg tatcatcaag attgttaaga
    10201 acgaagctac aaaaaccgat agctattaac ttaatcatac cgatttagct tgagactaaa
    10261 acaaaaaact catataatag gagttaaaaa gcctactatc ttagtgataa taggcttttt
    10321 tagctatgaa gacttccctg cgatccatcc tgaacaaaga gctgaggtaa tattaaaccc
    10381 tccggtgtga gcattaatat ctaagacctc tccagcgaaa tacaggccag gaactttctt
    10441 gctttccaag gttttagggt tgatttcttt taaatctacg cctcctttgg tcacaaatga
    10501 tttagccaaa gacatttttc ctgtaatggg tatctggagg tgtttgagtt tatcaagcaa
    10561 ttccttttct tgttttggag agagttgttt gaccttttcg gggtaatctt ctgataaaaa
    10621 atcagctact cgttcaggca gtaggccttt aagagcattt ttgatgtttt tatcacgttg
    10681 atcactcaga taagcggtta agtcatctgt tgataagtgt ggcaaaaagt ccagctctgc
    10741 aatttctcca cctttcacaa aagaggacag acgaagagct gcaggtcctg aaaggccaaa
    10801 gtgggtgaag agtaagtcgt gtgtgatgac gtgtttgtca taactaagag taacgtcgtc
    10861 tagggaaata ccttgcaaga ctttgtgagg aaaatcagtt aacagagggc tctcagcagc
    10921 ttctaaatca gtcacggtca gtttaaaatg ccgtgcaata tcatgtccaa aacctgttga
    10981 acccgttgaa gggtaggact tgcctcctgt ggtcacaatc aatttttggc aggtgaaagt
    11041 ctggtctgca gatttgaggt agaagaggtc atcttgtttt ttaacggaaa ccacttcagt
    11101 gctggttagc acctgacccc caagagcctt gatttttttc tccagagcat caatgattgt
    11161 ccttgattta tctgttgtgg ggaacatccg tccatggttt tcttctttga gtttgacgcc
    11221 gttttcctcg aaaaaggcaa tgatgtcatg gttgtcaaac tgggaaaaaa cactatataa
    11281 aaagcggcca tttccaggaa tgccagccat aaggtcatca aggttaccac tattagtgac
    11341 attacagcgt ccaccaccgg ttcccgctaa ttttttacct aagcggcgat ttttttcaat
    11401 taagagagtt ttatagccat agtagctgct ggagatggcg gccatcatac cagcagggcc
    11461 acctccaata ataatcgtat cgtattgagt catagcctta ttttagcata aaagcggggg
    11521 gggggttaat agtcaggtaa atgaactaac ctggcatacc gtttttttac gcatttaaac
    11581 aatgcctcca tgacatcaaa aagctagaaa aatagtccag atgattaaaa ttatctttgt
    11641 atgaaaacgg ttgctttttt atttaattca tgctattgta ttactaaaga atgaagtaat
    11701 cattaagttc ttttgaaatt ttttaaaaat taaataattt tattaaagga gaatagtata
    11761 aaaaaagatt taatagcaaa gcgataactt tgcttgctgt aagctcttta agtttgggat
    11821 ttacggccgt aactagtgtt gtcagtgcgg caacagcaca tccagctcca tctccaatta
    11881 ttgatgaatg gaattatgga gtaagtgaca actatggtta ttctaattat tatgttaaat
    11941 ctccaaacaa tattggttca aaatcttcga taactaatta ttggggatcc attaaggcat
    12001 ctgattctca aaaatacggg tgggcactgt caagttcaaa aaaaagcttg gaatgatatt
    12061 cggttgaatg cacattggaa ttactttaat ttttagaaat ggtatatgca agctaaagac
    12121 ccaataatta ttatagggtc ttttataata taggaagttg tttatgaaat taaaattatc
    12181 tgtcattgga ttattctttt gcttaattgc tgcgataggt gttatcagta ttagtgatac
    12241 tcaaaaacca gtacctttac caattgatgg tgccttttca atccaaggaa aaagtagcct
    12301 ttctagtgac gaagtttaca aaaagattag taatctatca aaaacgaaaa acgttaatat
    12361 ttacaagcct atagttcgta actcaggtca gttgacttat atcaatttta aaaatacaaa
    12421 ttatgaacaa ttaaaacctg caccgataac aggcatgtat tataccttag gtaaaataga
    12481 ttcaagtagc ttaaaggtac ttacagcttc aggtttgaaa cttatttatg cagcctatcc
    12541 ttggtatata ggggggattt tacaatttga tgggacttta aggttgttat tgatgctatc
    12601 aatttactta acattattag ttgttttatt tgttgttaga acgaggcaga ttaaagaagg
    12661 agtgataaga cattctttag gattgcctat atataactta agaagagatt atgtcatttc
    12721 ttttactttt gaattggtta tggctggttt gttaatgatt gcctacagct atttttgggg
    12781 aagtggcctc tttacttaca gttctaagtt atttttctct ctaattttaa ctaatcttat
    12841 aatattccag attgttgaca ttatcacttt tattttattt tggctaacta ttagggttga
    12901 gaaacctatt gaaattataa aaaataaagc aaaaaatagt tttcttttta ttgtatggct
    12961 tgtaattatt tctattataa taatcgtctc aggaatcttt ttacaagaaa caaaggctag
    13021 tcaatcaagg ataaatagac agataagtca actatcccca tggaacaaag taagaaactg
    13081 gaaaagacta gagttgttgg ggattgaaaa tggctctatt aataatgggg aaataaatga
    13141 gccagaatca caatatatac agatagcga
//
LOCUS       DAUCWU010000045        13074 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUCWU010000045 DAUCWU010000000
VERSION     DAUCWU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.045"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(58..1677)
                     /locus_tag="VGY24_001145"
     CDS             complement(58..1677)
                     /locus_tag="VGY24_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4284127.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(1984..3528)
                     /locus_tag="VGY24_001146"
     CDS             complement(1984..3528)
                     /locus_tag="VGY24_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ4284128.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(3776..4471)
                     /locus_tag="VGY24_001147"
     CDS             complement(3776..4471)
                     /locus_tag="VGY24_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ4284129.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            complement(4551..5942)
                     /locus_tag="VGY24_001148"
     CDS             complement(4551..5942)
                     /locus_tag="VGY24_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ4284130.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLYHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            complement(6141..7187)
                     /locus_tag="VGY24_001149"
     CDS             complement(6141..7187)
                     /locus_tag="VGY24_001149"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ4284131.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVIAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            complement(7288..7884)
                     /gene="recR"
                     /locus_tag="VGY24_001150"
     CDS             complement(7288..7884)
                     /gene="recR"
                     /locus_tag="VGY24_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ4284132.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(8682..9461)
                     /locus_tag="VGY24_001151"
     CDS             complement(8682..9461)
                     /locus_tag="VGY24_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ4284133.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIASSPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(9585..10127)
                     /locus_tag="VGY24_001152"
     CDS             complement(9585..10127)
                     /locus_tag="VGY24_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284134.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFFAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            complement(10288..10809)
                     /locus_tag="VGY24_001153"
     CDS             complement(10288..10809)
                     /locus_tag="VGY24_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ4284135.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQESFLAIK
                     HDLELAGILYKGI"
     gene            complement(10917..11612)
                     /locus_tag="VGY24_001154"
     CDS             complement(10917..11612)
                     /locus_tag="VGY24_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ4284136.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            11851..12786
                     /locus_tag="VGY24_001155"
     CDS             11851..12786
                     /locus_tag="VGY24_001155"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ4284137.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
BASE COUNT         4057 a         2688 c         2248 g         4081 t
ORIGIN      
        1 aaaagcaact ttttcagaaa attaaaaaga gctgactctc atcaactctc tttaagctta
       61 ttcacctttg tgacgaatca caaaaccagt atttttagca cttgattttt tctctttatt
      121 gaaaaagtct ttactattac gttttgattt acgtttgaaa tcgcggttgc cactgccatc
      181 gtgaccatca cgcttatcac gtttttgacg acgacggtca ccatcacgct catctctgtt
      241 acggtcacca cgatagccgc cacggcgatc accacggtta cggttgtcac gtccacgacc
      301 acctttgcca tttttattgc cgtgaccacc acccacatat ttgaatggta atggtttttc
      361 acgtgcaatt tcaacttccg gtaagctatc tggatcttgg accgttaagc tcaaaatata
      421 taaagctaat tcttcaggag tgaattcggc agccaattgg acagcatcgc ctttgaattt
      481 atcaaagttt gaacgaatag tctcatccgc aaagtcgcgt tcaattttct tgagggctac
      541 ttttttcttg gcttgaaagg cctcttcagc agttgctgga cgaagaggtt tcatctgttt
      601 tttcgttaag ttctcaatca ttgagaggta gcccatctca tttggtgaga caaaagtgat
      661 agactcacca gatttaccgg cacgacctgt acgaccaata cggtgaacat aactttctgg
      721 atcttgggta atatcgtagt tataaacgtg ggtaactcct gaaatatcaa ggccacgcgc
      781 tgctacatca gtggcaacaa gaatatcaat ttggtcgttt ttgaaatcac ggataacacg
      841 taagcgttta ttttggtcaa gatcaccatg gattccttcg gcacggaaac cacgaagttt
      901 caagccacgt gtaatttcat caactcggcg cttagtccgc ccaaaaacga tggataattc
      961 tggctggtta acatccatca gacgggtcat agtatcaaat ttttcttgtt ctttcacacg
     1021 aacatagtat tgatcaacat tgacgtttgt taattctttg ttcttaattt gaacatgttc
     1081 agggtctttc atgaatttaa ccccgatttg tttaataggt gccggcatcg ttgcggagaa
     1141 gagcaaggtt tgacggtctg ctgggacacg actaataata gcttcaatgt cttccaagaa
     1201 tcccatgtta agcatttcat ctgcttcatc aaggattaag gtttccacat gatctaaaat
     1261 aagagccttg cgcttaatca aatctaacaa gcgacccggt gttcccacga caatgtgggc
     1321 acctgattta agggctttga tttgtttttc aatacttgat ccaccgtaaa cagagcgaac
     1381 cttaacccct ttttcacgtc caaaacggaa caattcttcc tgactttgta cagccaattc
     1441 acgcgttggt gcaatgacca atgcttggat gatattttcg ttagtacgaa tcttattcaa
     1501 ggttggtaga ccaaacgctg ctgttttacc agttcctgtt tgtgcttgac caataacatc
     1561 cttcccttct agtgcgagag gaatcgtcat ttcctggata ggagatgctt tctcaaaacc
     1621 agcggtaaca acagctgatt ggatgtcttg tgataagtta aattctgtaa atttcaattg
     1681 tttctctttt ctaaaaaggc agtgcgaagc tgccttataa accttttaat tttcgtcgtt
     1741 tgattaacaa cctaactagt gtaacacaat tagaatcaaa aagataggaa ataaggctaa
     1801 tataagcaat aacacgcttt cacgattatc cgctaaatca taaatatgct tacagttgtg
     1861 aagatatcgt tttttgatag aatcttaaat ctgcaaaaga gataactagc cactcatttc
     1921 ttttaaaaag ttgctcagta aattgatctc caaaacgtta gactgttgaa aatcatgttt
     1981 caattacatt ttctcttcta aagtgacatc tgggtatttg tctgcaaacc agcgaagggc
     2041 aaaatcattt tcaaatagga aaactggttg gtcaaaacga tccttagcta aaatgtttcg
     2101 gcttgaagac atgcgttgat ccaaatcatc ttctgaaatc cagcgaacag tctttttacc
     2161 cattggtgtc atcacaactt cagcgttgta ttcaccttcc atacgatgtt taaagacttc
     2221 aaactgcagt tgacccacag cacccagcat gtattcgcct gtctgataat tcttataaag
     2281 ctgaattgct ccttcttgta ctaattgttc aatacctttg tggaaggatt tctgcttcat
     2341 aacatttttc ggagaaacct tcatgaagat ttctggggta aaagttggta agggttcaaa
     2401 ttcaaattta tttttcccaa ctgttaaggt atccccaact tgataagttc ctgtatcata
     2461 aaccccgata atatccccag caacagcatt cgtcacattt tcccgtgatt ctgccataaa
     2521 ttgagtaaca ttggagagct tagcgccttt gccagtgcgt gtcaaattaa ctcccatacc
     2581 gcgctcaaat tcaccagaaa caatacgcac aaaagcaata cggtcacggt gtttagggtc
     2641 catgttagct tgaattttaa agacaaaacc tgaaaaatct ttagctaagg ggtcaacaac
     2701 attaccttct gtagtcttgt gaccatgagg ttctggggca aactccaaga aagtatcaag
     2761 aaaagtctga actccaaagt tcgtaagggc agagccaaaa aagactggag ttaggtcacc
     2821 atcaagaata gcttgttctg aaaagtcatt acctgcttct tgaagaagtt caatatcttc
     2881 tttgacttgt tcataaaagg gattattggc aaacagctga tcaccgtctt caatacttgc
     2941 aaaacgctcg tctcccttat agagttctaa acgtttatta tgtaaatcat ataagccctc
     3001 aaaggcgcgt cccataccaa tcggccagtt catcggataa gaagcaatcc ctaaaacttc
     3061 ttctaattct tccaagagtt ctaaaggctc acgcccatca cggtctaact tgttaataaa
     3121 ggtgaagaca ggaatattac gatgtttgac aacttcaaaa agttttttcg tttgcgcctc
     3181 aataccttta gccgaatcta caaccatgac agccgcatcc acagccatta aggtacggta
     3241 tgtatcttcc gagaaatcct catgcccagg tgtatccaaa atattaacac gtttacccgc
     3301 gtaatcaaat tgcataacag atgaagtaac agagatacca cgttgctttt caatatccat
     3361 ccagtcggac tttgcaaacg tacctgattt tttccctttt actgtccctg cttctctaat
     3421 ttcaccacca aaatataata gttgctcagt aatagtcgtc ttaccagcat ccgggtgaga
     3481 aatgatggca aaagtacggc gttttttaat ttcttctgta agtgacattc ttttttcctt
     3541 gtatagtaaa atggcagagc ttttctctga gttattttcg ttaggtatta tttgtttatc
     3601 aaaagagttg ggctacattg ggtttctcca tatacgatta gcctctaaag ttcttcaaaa
     3661 ctggtattat aacttatctt gggctatcaa tgagtgtctt ttttaaaaaa atcgagcctg
     3721 gaaatcatcc cagactcttc ttattatagc tgattttaag tgctttttca acgacctact
     3781 tttctttaga gaaagcaaat gccagctgtt gagcttcacc aaatttaagg taaaaatatt
     3841 cggttatttt aaccataaaa gtcaagccac ttgtaacgat cacaaaatta agtagcaaat
     3901 gacgcgtatg aatcactgga atatccatca caaaaccata atttcctttc gtggctaaac
     3961 tgaccaacag aagaagaaag ttaacagttg ctaaatagcg tactactgat agcagtctta
     4021 actgactggc atcgtaaaaa cgcaagaggt aaatcaaacc attcactaac aaagcataat
     4081 gaccaagata aaacgagaca tttgctacat gccataatct aaacggataa agatctggcg
     4141 ataaaagtgc aaggaatgtt ccacctatcc ccaaaaccat aaataattgc ttaaacttgt
     4201 tacgatcagg tagaaaaaag atagctaaca tagcgatgcg acaatgatac aacggcaaag
     4261 cttcatctaa aggaaacccc cttaacacgt accatgtgta caagccaatc acctgagata
     4321 actgtaaaaa caagaaaaaa taacgataag attttaacct atatgcttga aaagtcaaaa
     4381 aaacaagcaa aagcgctatc aagagtgacg acaagtaaaa gataagtgag gtatgaggca
     4441 gtccgatggg gtcaattgca aaaaaatcca taatcgtccc tttctatatt ttttcattat
     4501 accttaatct tcatgagaga acaagccttt ctctgaaagt tttataaaaa tcaaggctga
     4561 tagctgtcac aattcttgtc agtcactaat cgatctacta acttttctag cgacatcgag
     4621 tggctgcctt ttaaaagaat ttgatcaaag ggattaagaa tgttttgaac atattgtgcc
     4681 atggcttcaa actggtcttc ttgttcagtt tttaaaaaat aatgtacttg ctcagcagga
     4741 taaacctgac ttgccaaccg tgctaattct ttaatatgat ccccataaaa aacaagctga
     4801 tcaatattac ctgatgttaa gctgtcaatt aactgagaat gaagtatcac tgaatccttt
     4861 ccaagttctt tcatatctgc tagcactgca atcttttttc ctccggggtt ctttgctatg
     4921 ttagcaaatg tttctaaaat cagacgcatg gctgttgggt tagcattgta gacatctgat
     4981 aaaatatcag caccattggc tgcttttttc cactccgtac gattacctgt caacgtcaca
     5041 gtctgtaaag cagctataat atcctcgtct gttactgcca atagttttcc tacataggct
     5101 gctaccatag cattggtcgc gttgtatttt cctggtagag gtagggacac aggtgttgct
     5161 aacacatttg ttgtaaacgt taagctatct ttgtcttctt gaataccagt aacgtcaatt
     5221 tcctgctgat ttccaaaacg gataaccatc tgattttctg gaagatatgg gtcaataatc
     5281 ggatctcccg gagcaattaa aatcccatct gaattcatgc cgtcaacaat ctgcattttg
     5341 ccttgggcta ttttgtctcg gcttccaaaa tattctaaat gagcttcgcc gactaaggtt
     5401 aaaactgcaa tacgaggcct tgcaatttca gacagaagac gaatatctcc catgtgatct
     5461 tgtcccattt caagaacaat tttttccgta tcgtcaggca tgtggagaac ggtgtaaggc
     5521 aaaccaatct cattattgta atttccttgc gttttgtaag ttttgtaagt ggttgatagg
     5581 acagctccta tcatatcttt ggtggaagtt ttaccatttg agcctgtcac agcaataaca
     5641 tccacgcgca tcttatcaat ataataatgg gctagctttt gaaaagcttt taggcagtct
     5701 tcaacaagaa ggtgaggttt ccccggcaat tctttttctg aaaaagtagc gacagctcca
     5761 ttttgaaagg ctaaatcaat aaactcatga ccatcccttt gaccttttaa gggtaaaaag
     5821 aggtctcctt ttgtaatctt tcggctatca aattcaatgt gatacagagg aacgtcgtct
     5881 aagtcagaca cattattttg ggcatcaact attttagcca cttcgtgtaa ggttaatttc
     5941 attttgtttt ctttctaaga acactagtat ttacgatgtc ctatctatta tagcacaaaa
     6001 cattagaaaa atttctaaaa tgtcaaactc ccttaatggc tctactattc ttcctaaaag
     6061 agcaacaaaa aaaggacgaa cgtcacatct tgacgatgtt tgccgttcca tccaagtcaa
     6121 tgtttattca tctcataggt ttaaatcagg tggctctcac gctggtcaaa catttcttgt
     6181 gccagtgtca ccagctcttc aatcaaatct ggataagcca atcccatatt ttcccataag
     6241 agtggataca tagaccattg agtaaagcct ggcatggtgt ttaattcgtt aaggtaaacc
     6301 tgtccgtctt gggtcaagaa aaagtcacaa cgggacaacc cacaaccacc taaggcttta
     6361 aaggctactt cagcataaga acgcatttct gtcacaatac tctggtctac atctgctgga
     6421 atggccatcg taatcttatt gtcaacatat tttgcttggt agtcgtaaaa atcaacgtcc
     6481 ttgattactt ctcctggtag agttgattta accttatcat ttccaaggag accaacttca
     6541 atttctctgg caatcacgcc ttgttcaatc aagacacgac tgtcataggt cagggctaac
     6601 tgaatcgctt ttcttaattc tacttttgtt tgggctttgg aaatccccac agaagaaccc
     6661 atattagctg gttttacgaa gataggaaaa gtaagtcttg ctaaagtttc aactaaacaa
     6721 gcctccaaat cttgtccgtc aatataaaca gtataggcaa cttgtggaat tccaattgat
     6781 tctaagacac gtttagtagt aattttatcc atagctatgc tagaagacat gacattagtc
     6841 cctatatatg gcattctaag cacttctaag aaaccttgga tagagccatc ttctcccata
     6901 ggtccatgta atacaggaaa tacaactgca ccttcttcat agatatcact tggttttatt
     6961 ttttgagtta gctctatcgt ctcatttgtc atcaagcgtt ctgattccga tggcttttct
     7021 gaaaattgct gagttttgat aaattggccc atctgagtaa taaaataagt ttttactaag
     7081 aacttatcat agttgactgc tcgcatcaca ctctctgctg aaagcactga gacttcacgt
     7141 tctgctgagc ggcctccgta taatagaact aacgtttgtt tggacatagc taacctttct
     7201 tatctgaatt gaatgttttt ttattataac aaaaagatgt agccttttca aaacctctct
     7261 ttgcgagtaa aggcaccaac atgatcatta aagttcagta cgattctcaa tagctctcaa
     7321 taatgttact tcatcagcat actcaatatc tgaacctact gcgagacctc ttgccaaacg
     7381 agttacctta atcccggctg gtttcaagac acgtgaaata tacatggacg ttgcttcccc
     7441 atctgctgtg gcattggtag ctacgataac ttcgctcacc ttaccatcca ttagacgggt
     7501 aattaaactt ttaaggttga tgtcatctgg ccccacacca ttcataggcg aaatcaagcc
     7561 gtgaagcaca tgataatagc catgatactc ttggattttt tccatggcag aaacatcttt
     7621 agcatcttct actaccagaa tggtcgtctg atcacgactc gtgtctgtgc aaatgtgaca
     7681 aggatcgtca tcggtaaggt ttccacaaat cgaacaatag gtcagttctc ttttagctgc
     7741 taataagttt ttagcaaaat cattgacatc ttcatttgac attccaatag tataaaaggc
     7801 taatctcgtc gccgtcttga taccaattcc tggaagttta gagtaactgt caattaactt
     7861 tgcaatgggt gttggataaa gcactaagtt ctcctttatt gttttccata tatcgccaca
     7921 taaagcgccc tcacatcttt gccgatatat tcgtaatatg tggatacata acagcaaccg
     7981 caatattatc ttcgttattt gtattataag ctactacatt taagttgtaa gttactaagg
     8041 tctgatctga attgtctgtg atacaagtct cagcagttcc aattttagcg tgaatcgtga
     8101 cagaaccgtt tggattagcc aagtaaaaac ttttttactc gacttgttac cttggccttg
     8161 agatatttgg cttttgtttt ttgattcatg ttactttttt agaaaccgca aaccatcctt
     8221 atgatataag tttataggaa ttgtttcatt ccatgcagaa atgatgttta ctcaattgac
     8281 tatgctattt ttctcaacaa tagtgcataa agcaaactca tggttatctt taagctttca
     8341 tgctacactt tcttcactaa aagtatatta ccaaaagcag tattaagaaa tctctaagca
     8401 caaaccataa gacagtttgt acgcctgatg aatctgttgc tgcatcactg tccacttttt
     8461 accctatcaa ggctattttt gaaagacaac taatgatctg cattattttg ggaactactc
     8521 ccatagcaaa ttctttttac ggattttttg ggaatactca ttttttaggg aataaactaa
     8581 ccattattta gctaaatttt ttaaacttcg cttcttattt taccataaat tataaaaaga
     8641 agactgagaa tagaacgccc tcagtctaaa aaaatatttt ctcattgagt tttactcttt
     8701 tgcggatgat agtaaacttt aaaatataaa aaactaacaa aaatggctcc accgataata
     8761 ttaccactat agacaaaaat aaaattagtg gcaaaatcca accatgttgc tcctccttca
     8821 aaaatagcag caggaatgac aaaagcattt gctacactat gctgaaaacc aagggctaca
     8881 aaggtcatga caggaaacca agtaccaagg aatttaccag cagcatcatt tgccccatag
     8941 catagccaca aggccaaacc aacaaaccag ttacaaccaa ttcctgatac tagtgcctgc
     9001 aaagggctcg atgctatttt agcatgggcc acctcaatca cttcttcttt aaaaatacca
     9061 gctgatgtca aacctagaaa atgaccaaac acaaaagcta caaaaacagc tccaatcaca
     9121 ttaaacaagg taatgataag ccaattcttg gcaagctcgc taaacttaat ttttttggcc
     9181 aacaaagctg cagaaacagc catcatgttc cctgtaatta attctccacc cgccattaaa
     9241 ataataatta gaccaatcgg aaaagcacaa gctcctacga tactggaaaa agcaccaaaa
     9301 gtttctaaac cactggcggc aattctcacg tatagtaaat aaccaaggct aatcatcgct
     9361 ccaccaataa agcctaagat agatttagcc aaaaaagttt tggtaacctt atgttcccca
     9421 atatgaatag ttgcttctaa aatctgttct ggtgttttca tgatatctcc ttttgttact
     9481 gtattcacaa acacacactt tagtatatca attttcctaa aaaatgcaag ccctttcaac
     9541 aagaaagggc tcgtcattat catataacta tctctatttt agtcttaggc atttatggtg
     9601 ggaagtttta agttttttgc taaaaaacgc catagaacta aagcaagata gaaatccaat
     9661 atgccatgaa taagcgaacc taagccaatc aagcctagca tagaccaaaa ataagacata
     9721 gaagttgctg tggttgctgt taaaatatag acaactaaaa attcagccaa gccatgaatg
     9781 acattaatga aaaacgcaaa actaaaacat tttactgccg atgtcatcag ttgtggtttt
     9841 ctacttaacc accacgctgc caaaatagca aacaataaat gagagagtgc tcttaaaaca
     9901 ataattaaag gaagtccagc taataaaaaa cctaatgtgg taccaagagc gaccaaaatt
     9961 gctactggaa tcgacataaa aatagcaaaa aacaaaggaa catggctcgc cagagtaaag
    10021 gaagctggtc caataataag cttaagaggc atgattatcg gaatcaaaat cgcaaatgcg
    10081 accaaaatag aggtataagc tattagttgt gttggttttt tagtcatttt atctcctttt
    10141 caaaaatagt ataccttaaa aaccaataac tatcaagaca cctgtatctt ttggtatttt
    10201 taaacgatac cttgttgctg ataaataatc aatagaatat tttagcatgg tattcataac
    10261 aaccatcaat ggcataatat actatagtta tattcctttg tataagatac ctgctaattc
    10321 taaatcatgc ttgatagcta aaaaggattc ttgactagga cacgaaatta agtgactatg
    10381 tagtccttct gtcaaggaag acaataactc tgctttagat tgactgagtt tggacatgaa
    10441 attagttaca tcagagtgtg tcttaatatt taaaggggct gttatcatac cgtaaatagg
    10501 atgttctacc tctaccgtcg taataatccc ttgatgagct aaaataattt ctaattcttt
    10561 tttagtttct tctacattat gttggcacac aatacgtgca caaaattggt gactataaag
    10621 cgctgttgac ataatataac cttttggagt cgaaataata tcaatctgct gagctcgaag
    10681 aagagctata tctccaacga ttacttggcg actcactcct aataacttac ctaaacgagt
    10741 agcagataca gctttttgtt cactatttaa gcactcaata attttttgac gacgatcttc
    10801 tgctttcata atctatctat ccttttttat ttctatcata tcatattttt aagcagggtt
    10861 ttctttcttc ttaggatata gaacccaaaa aaccagacca aagtctggtt tttaagttat
    10921 tcaccaccaa ggtagtattc tttaacaatg ttaagttttt catctaattc aaaaacaagt
    10981 ggtgggaagt ttggaatttc cacatccatg atttcgtcat ctgaaagacc tttaatgtgt
    11041 ttcacaagag cgcggattga gttaccatgt gcaccaacaa agacgttttt accgtcaaga
    11101 agagctggag caattttttc ttcccagtaa ggcattgcgc gttcaagagt tacttttaag
    11161 ttttcagcat ctggaataag ggctgggtca agatcagcat aacgacggtc tttgtgtgct
    11221 gaatactcat catctttagc catagctggc ggcaacacgt cgtatgaacg acgccagata
    11281 tgaacttgtt catcaccaaa ttgttctgca gcttcagctt tgtttttgcc agtcaaagca
    11341 ccatagtgac gctcgttcaa gcgccatgat ttttcagttg gaacccacaa ttgacctgca
    11401 ttttcaaggg caaggttagt tgttttgata gcacgtgtca atactgaagt gaaagcaagg
    11461 tcaaattcaa tgcctgcttc tttgatcaat ttacctgcat caattgcttg ttgtgtccct
    11521 ttttctgaaa gatcaacatc tgcccaacca gtgaagaggt tagctttgtt ccattctgac
    11581 tcaccgtggc gagcgaaaac caattttacc atgagttaat atctcctttt aatttaaagg
    11641 ttgctacctt tttacaattc ctattttaca aaaaatatag caaaaaagct agtctattct
    11701 gactttattg aaaatcataa tgtgactatc atgaaaattt caaatgttcg tcaataacag
    11761 taatatattt acatttttgt aaaaatagct tgctattatt catgcctagt ggtagaatag
    11821 aaaaaatttg tctaaaatat ggaggaaata atggtttcta ctgctacaca aattggtcat
    11881 ttctcttttg ataactgttt gatgaatgct gctggtgttt actgcatgac aaaagaagag
    11941 cttatggagg ttgaaaagtc tcaagccgct tcctttgtca ccaaaacagg gacgcttgaa
    12001 gttcgtccag gtaatccaga acctcgctac gcagatactc gtcttggttc tatcaattca
    12061 atgggactcc ctaataatgg ttttcgctac tacttagact ttgtcagtga tcttgccaaa
    12121 accgggcaac ataaacctca ctttctctcc gttgtcgggc tatctcctac tgaaaccgaa
    12181 accattttaa aagccatcat ggcttcagat tatgagggtc tagtggaact aaacctttct
    12241 tgccctaacg ttccaggaaa accacagatt gcttatgatt ttgaaaccac tgatcaactt
    12301 ctagagaaca tctttaccta ttacactaaa cctcttggta tcaaattacc tccttatttt
    12361 gacattgtgc attttgatca agcggctgcg attttcaata aataccccct ttcctttgtt
    12421 aactgtgtca attctatcgg aaatgggctg gtcattgagg atgaacaagt cctcatcaag
    12481 cctaaaaatg gctttggtgg tataggtgga gattacatca aacctactgc tctagccaat
    12541 gtccatgcgt tttacaaacg cttaaaacct tctatccaca ttattggtac agggggagtt
    12601 aaaacaggac gcgatgcctt tgaacatatt ctttgtggcg ccagcatggt ccaaattggg
    12661 acagcccttc atcaagaagg gccagctatc tttgaacggg tcactaagga actaaaaacg
    12721 attatggtag aaaaaggcta ccaaagctta gacgattttc gtgggaactt acgttacaaa
    12781 gattaacctt aatagtcaaa aaagcttgga aatgtgcact ttgtacactt tccaagcttt
    12841 ttaaaatggg aatttttttg aagggacagc gtcactttct tgggactgtc tttgctggtt
    12901 gtttgattca acttttcttt tttgcttgcg ctcaaattgc tgataaaaat agaaaaatag
    12961 aatgatttta attattgaaa atacagctaa accaatgatc ataatagtaa acatacggcg
    13021 gttttctctt tcataagtct tactattatt ataaacactt tcaagttttc aagc
//
LOCUS       DAUCWU010000046        12961 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUCWU010000046 DAUCWU010000000
VERSION     DAUCWU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.046"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            427..1143
                     /locus_tag="VGY24_001156"
     CDS             427..1143
                     /locus_tag="VGY24_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4284138.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            1346..2188
                     /locus_tag="VGY24_001157"
     CDS             1346..2188
                     /locus_tag="VGY24_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4284139.1"
                     /translation="MIKKQYLGRATIGLASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSGIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            2515..3360
                     /locus_tag="VGY24_001158"
     CDS             2515..3360
                     /locus_tag="VGY24_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ4284140.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            3610..4674
                     /locus_tag="VGY24_001159"
     CDS             3610..4674
                     /locus_tag="VGY24_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284141.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKAFHEAGVDVSILKRGA"
     gene            4675..5367
                     /locus_tag="VGY24_001160"
     CDS             4675..5367
                     /locus_tag="VGY24_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284142.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(5421..6791)
                     /gene="brnQ"
                     /locus_tag="VGY24_001161"
     CDS             complement(5421..6791)
                     /gene="brnQ"
                     /locus_tag="VGY24_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ4284143.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEMTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            7025..8239
                     /gene="sstT"
                     /locus_tag="VGY24_001162"
     CDS             7025..8239
                     /gene="sstT"
                     /locus_tag="VGY24_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ4284144.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLVLVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(8294..8968)
                     /locus_tag="VGY24_001163"
     CDS             complement(8294..8968)
                     /locus_tag="VGY24_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ4284145.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(8978..10369)
                     /locus_tag="VGY24_001164"
     CDS             complement(8978..10369)
                     /locus_tag="VGY24_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ4284146.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSLEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(10439..11152)
                     /gene="rsmG"
                     /locus_tag="VGY24_001165"
     CDS             complement(10439..11152)
                     /gene="rsmG"
                     /locus_tag="VGY24_001165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ4284147.1"
                     /translation="MTPQDFYRTLEKDGFLLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            11302..11859
                     /locus_tag="VGY24_001166"
     CDS             11302..11859
                     /locus_tag="VGY24_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ4284148.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            11906..12802
                     /gene="htpX"
                     /locus_tag="VGY24_001167"
     CDS             11906..12802
                     /gene="htpX"
                     /locus_tag="VGY24_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ4284149.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
BASE COUNT         4284 a         2193 c         2565 g         3919 t
ORIGIN      
        1 cttcagttaa tccaatgagg aaattttata acaataatta aaaaagtcag tcaaaagata
       61 tgctttcgaa gaacaggcta acgtttttac aagattatat cagtaatcat tttgacaatt
      121 gtagtaatgg aatgttacca aaatttagaa cgttgtgtgt tatggatgac aagattttaa
      181 aggaatacta attaaacggg aggtcgtgaa ccggaagctg ttgtcactca agcgccaaaa
      241 tctgtctgta acataatatt gatgtatggg aacgcaatta tttggttttt attgtgatgc
      301 ttaaaacgat tttatatttc tattttacca aagcttttca aagtgctata ataagggagt
      361 tagagtcaat attagtaacg ttaatgttga caatattcaa taaaatacta ttaaaaggag
      421 acaaaaatgg gacgtaaatg ggcaaatatt gttgctaaaa aaactgctaa agatggagca
      481 acatcaaagg tctatgccaa atttggtgtt gaaatttatg tggctgccaa acaaggcgag
      541 ccagatccag agttaaatac agctcttaaa tttgtcattg atcgtgcaaa gcaagcacaa
      601 gtgccaaagc atgttattga taaagcgatt gataaagcta aaggaaacac agatgaaact
      661 ttcgtagagg gacgctatga aggttttggt ccaaatggtt caatgattat tgtggatact
      721 ttgacatcaa atgttaaccg tacagcggca aatgtacgta cggcttacgg taagaacggt
      781 ggcaatatgg gagcttcagg atctgtatcc tacttatttg ataaaaaagg ggtcatcgtt
      841 tttgctggtg atgatgctga cagtgtcttt gagcaattac ttgaagcgga tgtagacgta
      901 gatgatgttg aagcagaaga gggaacaata acagtttata ccgccccaac agatcttcat
      961 aaaggtatcc aagcacttcg cgataatggt gtagaagaat tccaagttac tgaacttgaa
     1021 atgattcctc aatcagaagt agtattggaa ggtgatgacc ttgaaacttt tgaaaagctt
     1081 attgacgcac tcgaatcaga tgatgatgtt caaaaagtgt atcataatgt agcagatttt
     1141 taaattgaca tagaataata aaaagagttg aaatctattt cagctctttt taagtgatgc
     1201 ttttataagt aagtaccagg taacatagtc gttgttagaa gataatgaac aatagtaaat
     1261 cagtatcaac ttgataggaa tcaattatta gtgaactcta attaaaaatg gtaaactaga
     1321 cacaagaaca aaaaggagag ataggatgat taaaaaacaa tatttaggaa gggcaacaat
     1381 tggcctagca tcaacccttg tgctagcagc ttgtgggtca tcaaaaacag ctgagtcagg
     1441 aaatcagggg agttctaaag aagtcttatt tgcaacggtt ggaacaacag ctcccttttc
     1501 ttatgaaaaa ggtggtcaac taactggcta tgatatcgag gtggctaaag cagtctttaa
     1561 aggatcggat gattacaagg tgtcctttaa aaaaacagaa tggtcttcta tctttacagg
     1621 tttggactca ggaaaatacc aaatgggtgg taacaacatt tcctttacca aagagcgttc
     1681 agctaagtac cttttttctt atccaatcgg atcaacacct tctgttttag ttgttccaaa
     1741 agacagtggt atcaagagct ttgatgatat ccaagggcat acgacacaag ttgttcaagg
     1801 gactacaagt gttgcacagt tagaagactt taataagaaa cacagtgaca acccggttac
     1861 tctaaaattc accaatgaga acattactca aatgttaacc aatttgagtg aaggcaaggc
     1921 agatttcaag atttttgacg ctccaacggt taacgccatt attaaaaatc aagggttaga
     1981 taatctcaaa accattgaac tcacctcaac agagcaacca tttatttatt ttatctttag
     2041 tcaagatcaa gagaaattgc aaagctttgt taacaagcgt attaaagagt tgactgctga
     2101 cggaactctt agcaagcttg ccaaagagca ccttggcggt gattacgtgc catcagataa
     2161 agagttgaaa cttcctacag ctaactaaac catctattaa ccagttattc atctagaaga
     2221 ctggtttttc tgatcggaaa caatattata gtttatgagg ttatgttata aaaattgcaa
     2281 atagccttaa tcaaaatgag atgttaaagt gctttttgag aagtgaatat tgtaagaagt
     2341 atgctttggg ttttagtcct caaataccaa gttttttgtt gactaaacaa ataaggatgg
     2401 ttagcatagc atatagttta aaactatggt atatgctagc cattatcaat ttgaaaacta
     2461 tatctaatta agatacaata atgatcgtaa acaaaatgaa tcgaggataa cgctatgtct
     2521 tttaaaaaat ggattggaat agtcggaatc gctttagcat caactgtatt ggttgcttgt
     2581 tctgaaaaac aagatgataa aaacactcta actattgggg tgatgacaaa aacagaatct
     2641 gatcaagcga gatgggataa agttgaagaa ttattgaaaa aagacaatat cactttgaag
     2701 tacaaagaat ttacagatta ctcgcaacct aataaggcag ttgctaatgg tgaagtcgat
     2761 attaatgcct tccaacacta taatttctta aataactgga ataaggaaaa taaggaacat
     2821 ttggtggcca ttgctgatac ctatatcagc ccaattaacc ttttctctgg aaccagtcaa
     2881 gatgggaagg ccaaatacaa atcagtagct gatctgccaa atggtgctca aattgcagta
     2941 ccaaatgatg cgaccaatga aagccgtgcc ctctatgtgc tgcaatcagc tggcctcatt
     3001 aaattgaatg tgtctggtga tcaattagca accattgcca atatctcaga gaacaaaaag
     3061 aaattagata tcaaggaatt agatgctagc caaacggcac gtgctttagt atctgctgat
     3121 gcagctgttg tcaataatag ttacgctgtt cctgcaaaaa ttgactacaa aacctctctt
     3181 ttcaaagaaa aagcagatga caattcaaaa cagtggatta acattattgc aggtcaaaaa
     3241 gattgggaaa aatcagagaa ggctgatgct attaaaaaac tcatcaaggc ctaccaaact
     3301 gatgaggtga agaaagtcgt ggaaaaaact tcaaatggta tagatgtatc cgtatggtaa
     3361 attttagcca ttgttataac aagagtgtgg tcgtgtttaa aacacatgct ttcttgcatg
     3421 cttgaggaag agtttacgac taatttacct ttagaaagct atttaagacc ttttcccttt
     3481 taggatttag aaaccttggg aagactattg catgttatct taatagtcat gattatgcag
     3541 gcgatgaaaa cattgctatc gtagattact gtcgccatat cgtactaata gaggagttat
     3601 taaaatctta tgaatgaagc aataattcaa ttagatcata ttgatattac cttccgtcaa
     3661 aaaaaacggg ttatcgaagc tgttaaagat gtcacggttc atatcaacca aggagatatt
     3721 tatggcattg ttgggtattc aggtgccggg aaatcaactc ttgtgcgtgt gattaacctg
     3781 ctacaggcac caacaaacgg gaaaatcact gttgatgggg atgtgacctt tgaccagggg
     3841 aaaattcaat tgtcagctga tgcccttcgc caaaaacgac gtgatattgg tatgatcttc
     3901 caacatttca atttgatggc ccaaaaaaca gccaaagaaa atgtagcatt tgcgctccgt
     3961 cattcgtcgt taagtaagac tgagaaagaa cacaaagtta tcgaactgct agaacttgta
     4021 ggtttatcag agcgagcaga taactaccct gcccagttat cgggtgggca aaaacaaagg
     4081 gtggctattg cgcgtgctct agctaacgat ccaaaaattt tgatttcaga tgaggccaca
     4141 tcagcactag atccaaaaac cactaaacaa attttggcgc tcttgcaaga attaaaccgc
     4201 aaacttggcc ttaccattgt tatgattact catgaaatgc aaattgttaa ggatatttgt
     4261 aatcgtgttg ctgttatgca aaatggtgtt ttgattgaag aaggttctgt ccttgatatc
     4321 ttttcaaatc caaaagaagc cttgacacag gaatttatca caacagctac agggattgat
     4381 gaggctttag aaaaaattaa ccaacaagac attgttaaac atttgcctgc aaatgccctt
     4441 ctagctcagt tgaaatatgc aggaacttct acggatgagc ctctacttaa tagcatttac
     4501 cgtcagtttg aagtgacagc taatattttg tatggcaata ttgagatttt agatcatatt
     4561 ccagttggtg acatgattgt tgtcttggaa gggcaagctg aaaatattct tgcggctgag
     4621 aaagcctttc atgaagctgg tgttgatgtt agtattttaa agagaggagc ctagatgtca
     4681 cagttaattc aaacttattt gccaaatgtt tatgaactcg gatggtctgg agatgctggt
     4741 tggggcttag cgatttggaa tactttatac atgaccattg tgccttttat tgttgggggt
     4801 gctattggtc tcttattagg cttgttatta gttttaacag gaccggacgg tgttattgaa
     4861 aacaaaacga tttgttgggt tattgataaa gttacctcta tttttagagc cattcctttt
     4921 gttattttga ttgctatttt agctagcttt acctatttgc ttttaagaac aactttagga
     4981 gcaacagcgg ccttggtacc tttaaccttt gcgacttttc ctttttatgc ccgtcaggtc
     5041 caagttgttt tttcagaatt agacaagggt gttattgaag cagcacaagc ttcaggtgct
     5101 actttctggg atattgtcaa agtgtatctc agtgaaggtt taccagatct tattcgtgtt
     5161 tctacagtca ccttaatttc tttagttggg gaaactgcca tggcaggagc tatcggtgct
     5221 ggaggattag gaaatgtagc gatttcttat ggttataatc gttttaacaa tgatgtgact
     5281 tgggtagcga ctattattat tcttttaatt atttttgcta tccaatttat tggtgatagc
     5341 ttgactagaa ggtttagtca taaataaacg aaacaacacc ctaagttttt acttagggtg
     5401 ttgttgttta gatgatgtca ttagcttttc ttcgttttac ttagtataag ccctattaaa
     5461 aaaccaatag cagcaaagat aatccatccc attgagaatt gccctagtgg aacagttttt
     5521 tggaaaaaag ttacaacggc ttctggtaaa tgaaataatc cagtcatttg tgatagtgtt
     5581 aggagtgcat caaatatggc aggaataaag gttagaccaa ttgtagttcg atagacaata
     5641 ggatcattat tgaagcattt ttgtgccaaa acaaggaaaa ttaaatcgac tgttaatggg
     5701 tataaaagga ataaaacagg agctgaccag ttgataatga ctgataagcc accaaaataa
     5761 aagaaagctg atagtaaagt aaagatagtt gcccaagcaa tatgagataa agcaggcact
     5821 aatttatgga agtattcagc acttgacgtg attaagcctg ttgaagtggt tagacaggcc
     5881 aggaaaatca caacagctag aaatgcttgt ccgatgccac ctagataaaa acgagaagca
     5941 tgacttaaga tttgaccgcc attaactgga ttaccatgaa gggtaaagct gccatcacta
     6001 aaaggaaata atgattgtga tgttgctcca atacgaccga caaagatata gacaagtgct
     6061 aatagcaaaa tggcaatagc cccagaaata agtgttattt tggtcatttc tttgtccgtc
     6121 ttagcgccaa attgtttggt agcctcaatg actaaaatag caaaaacaag agaagctagt
     6181 gcatccatag tgccataacc ttgaattaat cctgctataa aaggaaaatc tttaaaggca
     6241 ttattaacac cgaccccagc gttaaaagca tcaccgtaat ttccagcagg atggacaaag
     6301 gacgcgataa ccaaaataga aataacaact aacaacgttg gcgttaaaaa ttttccgata
     6361 ttttcagcta gtttactagg tttgatagca aggaagtagg acagaccaaa aaatagtata
     6421 gcataaatag ctttattggt tgtattatct cctaagatag gagcgatacc gactgaaaaa
     6481 gaagtggctc ctgttcttgg aatagcaaaa aatgggccaa tagataaata caaaatggaa
     6541 gaatagaaga ttgcatacca tttagagatg gggcgtgcca aactttcaac atcgcctgat
     6601 ccagatttag caacagcaat gacaccgagt aaaggcaagg agacaccagt taaacaaaaa
     6661 ccaatgatag accatagaat ctgatgacct gagtagatgc ctaaaaaagc tgggtaaatg
     6721 aggtttgctg ctccaaaaaa tagcgcaaat agcatgaatc caatgacgat atatacattt
     6781 ttttgtttca tttataaata gtctttctat tgatactaat ccgaagattc taacaatttt
     6841 aattatatta tgtgtcaaaa taacaacaaa ggttattata tctaagatta cttcgatagt
     6901 caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt agctcattcg
     6961 atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag taaggatgta
     7021 acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa aaataggtat
     7081 cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag gttttagtat
     7141 tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg tatttgcttt
     7201 ggtttcccaa gctatctccc atcagaaaaa agggaaacag actaatatga cattaatcat
     7261 tgttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga cagcttatct
     7321 attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac cacctcaagg
     7381 tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga tcaatgcttt
     7441 agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac tagcattaaa
     7501 ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta cttcacaaat
     7561 agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt tcactacgat
     7621 ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttggtactag ttttagtagg
     7681 aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga ttacacgaca
     7741 aaatccttat ccccttgtct tgagatgttt acgtgagtca ggtcttacag cattttttac
     7801 acgaagttct gcagccaata ttccagtcaa tatgcaatta tgccaaaaaa ttggattgag
     7861 taaagatact tattcagtgt caattccact tggagcaact attaatatgg gtggggcagc
     7921 aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc ctattgattt
     7981 tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg cttctggtgt
     8041 tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca tttcaaatga
     8101 tcttgcgatg caggtggttg gtgttggttt tattgttggt gttatccagg attcttgtga
     8161 gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa atgctttttg
     8221 gaaacgtaaa aaagcctagc taggtttaac atagtaaaaa acatccttgg aaaacgccaa
     8281 ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag gtgtggtcat
     8341 tagcgatggc cacaatgata gtattcggtt ctaaaggttc gaaaggtttg acattggtat
     8401 ctaaggtttt aacttctttt tgacgcatgc caatcacatt taactcatat ttacgacgaa
     8461 catctaactc tgaaagagat tgtccttccc agcttttagg aatgacaaat tcaatcatcg
     8521 aaataccatg ttccaaatag atgatgcttt cgatatgacg tcttaacaga tttgaagcta
     8581 cacgttttcc agaatcacgt tcaggggtga tgactttagt tgctccgatg ccatatagaa
     8641 cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg agttttttac
     8701 agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata acgacagtat
     8761 cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct ccaacagcgg
     8821 ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca atagcaataa
     8881 cgtcttgatc aaaattactt aattctctgg caacagtacg gccaaaaata ccaaggccaa
     8941 gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa atatcagtag
     9001 tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca gtaattgggc
     9061 caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta gacaattgtg
     9121 gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca aataagagtg
     9181 gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc ataagaactg
     9241 caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg ataattcggt
     9301 tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag aaagtaatgg
     9361 cagcagtcgt cactttaata ccaccagctg ttccaccagg agcaccacca ataaccattt
     9421 gaatcatata aagaatgtta gttggtgcta gggtatcgtt ataagagatt gttgcaaagc
     9481 cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga tgtaaactaa
     9541 aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag gttccaagaa
     9601 aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat ttacgaaaag
     9661 ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact cctaaatcaa
     9721 cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata acatttaatg
     9781 taggatttaa cataaagtct tttaaactag aagatcctaa attgtcaaaa ccagcgttac
     9841 aaaatgccga cacagctaaa aaaatactat tgaagatacc atttttccac ccgaagcggg
     9901 ggatgaagtc aatcatgata actatcgcag caaaggcttc tagactaaag gttactttat
     9961 aagcaaaaaa aaggtagtgc tttaaatctt tgctatcacc tcgatttaac gctgattgga
    10021 gcaaggtttg atcgctcaat cgcatttttc gcttgagggc aaaagtactc acagctatta
    10081 acgtcaccaa gccaagacca ccaatctgca ttagtgccat agcaatggtt tgtccgatac
    10141 cgttatacac ttcagcgacg ggaacaacag aaagtccagt cacacaaacc atggaaacca
    10201 cattaaagaa atgatcaaga tagacggtat taggaccatt ttgataatgg gtaaagggca
    10261 tggataacaa gagagtccca atcagaatca caatggcaaa actaaatgtt aagcgctgtg
    10321 tgactgaaag agatttgata aaagagcgtt tcatgtttcc tccagacata atatctccta
    10381 ctataataac aaaaataagt taaaaaagct aagtttcatc ttagcctttg gtactatttt
    10441 atagaggttt tttgttaggc aagccagctt ttcttggata cttgttaggt gtttcctttt
    10501 tcttctcaac aatggtgatg aagcgagaat ctccatttgg caattggtag ctatggtttt
    10561 taatgacttt accaaaaaga agacaaagag cattcttagc ttcttctaac tcttggtcag
    10621 cagcttgtgc ctttaaggcg attaattttc ctccaatttt taaaaagggg atggttaatt
    10681 cagatagaac ctgcatccga gcaaccgctc tggcagtaac gacatcaaat tgacctcgaa
    10741 aggccttgtc ctgtccgaaa tcttctgctc taccatgaaa aaaatggaca ttttccaagc
    10801 ccaattcctg agcaaggagt gtcagaaaag aaatccgctt attaagagaa tcaataatgg
    10861 taacctcaag gtttgggaaa agaattttca tgggtaggct aggaaaacca gctccagctc
    10921 caatgtccaa taatttgatt ggttcattag ctaaaaagcc ctgtaaaata ggagcgatgg
    10981 aatcataaaa atgcttgagg taaacctcat tttcttctgt aatagctgtt aaattaattt
    11041 tagtgttcca ttccactagt aatttgaagt aggtgtcaaa ttgctctttt tgtttcgatg
    11101 ataataaaaa accatctttc tctagtgttc ggtaaaagtc ttggggtgtc attagtgtcc
    11161 tctgtttttg atagttgtta ccattttatc ataaaatagc ctataacaaa atccaataaa
    11221 tcatcagaaa taacacctca ttacttgcta ttagcctaga aattgctata attgagacac
    11281 aaagattaaa ggagacccac tatgccaaca gttttgatta tcttagtcgt tttaggagtg
    11341 cttgcgctat ggttaatgat tagttacaat agcctggtta aatctcggat gcacacaaaa
    11401 gaagcttgga gtcagattga tgtgcaatta aagcgtcgta atgacttgat tccaaatctt
    11461 attgaaaccg taaagggata tgctagctac gagcaaaaga catttgaaaa aatcactgat
    11521 ttgcgtgcgc gtgttgcgaa tgcctcaact cctcaagaaa ccatggcggc ttctaacgaa
    11581 ttgagtaaac aagtgaccag tttgtttgcc gttgctgaaa attacccaga cttaaaagct
    11641 aacgaaaact tcttgaaatt acaagaagag ttgaccaata cggaaaataa aatctcatat
    11701 tctcgtcaac tctataattc aacaacgtct aattacaacc ttcaattaga atctttccca
    11761 agcaatatcg ctggtaaatt atttggtttt aaaccaagtg aattcttaca aacaccagaa
    11821 gctgaaaaag aagttccaaa agttgaattt aacttttaaa tatgaaatta tcatctagta
    11881 ggcaatcata agagaaagga gaattatgct ttatcaacag atttcacaaa ataagcaacg
    11941 aacagttgtt ttattagttg ttttcttcgc tctcttagca cttattggag cttcagcagg
    12001 ctatctgctg ttagataatt atgccatggg cttggtcctt gctcttgtca ttggggtgat
    12061 ttatgctacc agtatgattt ttcaatccac cagtcttgta atgagtatga acaatgctag
    12121 agaagttaca gaaaaagagg ctccaggctt cttccatatt gtcgaggata tggctatggt
    12181 ggcccagatt ccgatgccga gagtttttat tattgaagat ccttctttaa atgcttttgc
    12241 gacaggatct agccctcaaa atgctgctgt tgcagcaacg acaggtttac ttgaagtcat
    12301 gaatcgtgaa gaacttgaag gtgttatcgg ccatgaaatt agccacatcc gaaattatga
    12361 tatccgtatt tcgactattg ctgtagcttt ggcgagtgct gttaccgtta tttctagtat
    12421 cggtgggcgt atgctatggt atggtggcgg ttctcgcagg caaagagatg acggagatga
    12481 tgatgtttta agaatcatta cattactttt gtcgttatta tctttgcttt tagcgcccct
    12541 agtagcaagt ttgattcagt tggctatttc tcgtcaaaga gaatatctag ctgatgctag
    12601 ttctgttgaa ctaacaagaa atccccaagg tatgattaag gctcttgaaa aattgcagtt
    12661 atctcagcca atgaagcatc ctgttgatga tgctagtgca gccttgtata ttaatgagcc
    12721 tcgcaaaaaa aggagcttca gttcattatt cagcacccat cctcctattg aggagaggat
    12781 tgaaaggtta aaaaacatgt gagcgtttgg ggtttttgct caagcctcac ttttttatgt
    12841 ctttgggcag ataatctaaa atggcaggaa ttatcaaaaa tatctgaaca aaaagaaaaa
    12901 aatgagtgaa acaatggtta aaaatagaat gatttaaatc ttaaaattaa aaaacgtcag
    12961 a
//
LOCUS       DAUCWU010000047        12580 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUCWU010000047 DAUCWU010000000
VERSION     DAUCWU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.047"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..771)
                     /locus_tag="VGY24_001168"
                     /pseudo
     CDS             complement(1..771)
                     /locus_tag="VGY24_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            759..2198
                     /locus_tag="VGY24_001169"
                     /pseudo
     CDS             759..2198
                     /locus_tag="VGY24_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2201..4378
                     /locus_tag="VGY24_001170"
     CDS             2201..4378
                     /locus_tag="VGY24_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ4284150.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQNPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            4375..5376
                     /locus_tag="VGY24_001171"
     CDS             4375..5376
                     /locus_tag="VGY24_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEQ4284151.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            5373..6305
                     /locus_tag="VGY24_001172"
     CDS             5373..6305
                     /locus_tag="VGY24_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ4284152.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            6550..6666
                     /locus_tag="VGY24_001173"
     CDS             6550..6666
                     /locus_tag="VGY24_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEQ4284153.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            6682..8601
                     /gene="tet(M)"
                     /locus_tag="VGY24_001174"
     CDS             6682..8601
                     /gene="tet(M)"
                     /locus_tag="VGY24_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ4284154.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLKALELEQEESIRFHNCSLFSVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            8720..8887
                     /locus_tag="VGY24_001175"
     CDS             8720..8887
                     /locus_tag="VGY24_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEQ4284155.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(8947..9300)
                     /locus_tag="VGY24_001176"
     CDS             complement(8947..9300)
                     /locus_tag="VGY24_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4284156.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            9805..10227
                     /locus_tag="VGY24_001177"
     CDS             9805..10227
                     /locus_tag="VGY24_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ4284157.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            10224..10454
                     /locus_tag="VGY24_001178"
     CDS             10224..10454
                     /locus_tag="VGY24_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4284158.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            10915..11118
                     /locus_tag="VGY24_001179"
     CDS             10915..11118
                     /locus_tag="VGY24_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ4284159.1"
                     /translation="MKQTDISIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            11200..12417
                     /locus_tag="VGY24_001180"
     CDS             11200..12417
                     /locus_tag="VGY24_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ4284160.1"
                     /translation="MSEKRRDNKGRILNTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         4188 a         2259 c         2542 g         3591 t
ORIGIN      
        1 ccaactttgt atagcagtat ttaaaaacca ttccgggagg tctattaagc cgctaaaata
       61 atttaataaa aaagagaatg taagataaat atacactatt ttacctagtt tcggtgccca
      121 tccaagagct aacgctgcca gacctataaa gaataaaaca gtagggaaaa ggttaaagcc
      181 agcagcaaaa aagtcaacta tatccatctc tccgctattc cccatcgcag agatagcagt
      241 acctccaaga ctacctgctg ctaataaaac tcctaataat ccgcttaata tcgataaccc
      301 tatactagtc caatataatt gactgcgggt tacttttgtt gcataaatct gacttaagtg
      361 taatcgtctt tcttcagaaa agagtttatt tacaatcgca attggtagaa tggaaactaa
      421 accaatcaaa accatcataa ctgtgcctgt gaatgattct tcaatagaaa atcctgtatg
      481 agaaaacatt tgtttcatca tttcattact ttctaggaat gtctgcatat ctccataaat
      541 cgaaccatag gcagcaccca taacaacata tgcgattaac caactaataa tgacaccttt
      601 attaatattt ataaataatc ctcggacgga cagcaaagac tgctttgcac tttcacgccc
      661 ttctctttgc ggtaaatatc cagcccccat atcgcggcca ccttcaagtg aaaaagctat
      721 gaacaccact attatactga aaattaaagc aaaaataagt ggaaaccaat ttagaccaaa
      781 gcaaggaatc tatgtacaag ctgtcttatg tggtaagggt atcagcaaat gatcttgacg
      841 aactcaaacg tcgttgtaat gaagtgaaag atttttatga cgatttaagc gtaaaactgg
      901 tacgaccatt tggggatatg ctcggcttac atgaagaatt tttacctgcc agcaagcgtt
      961 atatgaatga ttatattcaa tacgtgacct ctgatttcct cgctggttta ggttttggtg
     1021 ctactcaaat gctgggggaa aatgagggga tttatgttgg ctacagctta gatactggac
     1081 gcaatgtcta tctgaaacct gctcttgcca gtcaaggggt taagggttca gtaaccaatg
     1141 cgttagcgtc ggcttttgtt ggttcgctgg gtggtggtaa atcctttgcg aataacctta
     1201 tcgtctatta tgcggtgctt tatggggcac aagcagtgat tgtagaccca aaagcagaac
     1261 gtggcagatg gaaagaaacc ttgccagaga tttcccatga aatcaatatc gtcactctga
     1321 cttctgatga gaaaaacaaa ggcttacttg acccttatgt gattatgaaa aatcccaaag
     1381 attctgaatc actggctatt gatattctga cattccttac ggggatttcc tctcgtgatg
     1441 gggaacgctt cccaatcctt agaaaagcca ttcgtgcagt aaccaatagt gaagtacgag
     1501 ggttgatgaa agtgattgag gaattacggg ttgagaatac gccactaagt accagtatag
     1561 ccgaccatat cgaaagtttt acagactatg actttgcaca tttattattc agtaatggtt
     1621 atgtggagca gtctatcagc ttagaaaaac aactgaacat tatacaggtt gcggacttgg
     1681 tacttcccga caaggaaact tcctttgagg aatataccac tatggagctt ttatccgttg
     1741 ctatgctgat tgtcattagt acctttgctt tagactttat ccatacagac cgaagcattt
     1801 tcaagattgt agatttagac gaagcatgga gctttttaca ggtagcacaa ggaaaaacac
     1861 tatctatgaa gctggttcgg gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc
     1921 aaaatacaga cgacctctta gatgaaaaac tgaaaaataa cctcggctta aaatttgcat
     1981 ttcgttccac tgaccttaac gagattaaaa agaccttagc cttttttggt gtagacccag
     2041 aggacgaaaa caatcagaag cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg
     2101 atttatatgg tcgtgtcggt gtgatacagt tccaccctgt atttgaagaa ctgctccatg
     2161 cctttgatac cagaccacct gtgcgaaaag aggtgtaaat gtgaaaccat caatagtaaa
     2221 cagaataaaa tcaaactgga cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt
     2281 cacacttgtg attgccattt ttcttttagc catgctggga acggtggttc aagctgcggg
     2341 cttggtagat gatacggtca atgtggcaaa tgaatacagc cgatacccac ttgaaaacta
     2401 tcaactggat ttttatgtgg ataatagctg gggctggctt ccgtggaact ggtcggacgg
     2461 gattggaaaa caggtcatgt atggactata tgccattacc aattttattt ggacaatcag
     2521 tttgtatgtt tccaatgcga caggttactt agtacaggaa gcctattcct tagacttcat
     2581 ttccgctaca gcagattcca ttggtaagaa tatgcagacc ttagctggtg tgagtgcaaa
     2641 cggattttca acagagggtt tctatgttgg attcctctta ctcttgattt tggttcttgg
     2701 ggtttatgtt gcctatacgg gactgataaa gagagaaacc acaaaggcaa ttcatgccat
     2761 tatgaatttt gtgctggtgt ttatcctatc ggcttccttt attgcctacg ctcccgacta
     2821 cattaaaaaa atcaatgact tttcatcaga catcagtaat gccagtttat cacttggcac
     2881 gaagattgtc atgccccatt ccgatagtca aggcaaggac agcgtggact taatcagaga
     2941 tagcctgttt tccatacagg ttcagcaacc gtggctactg cttcaataca acagttcaga
     3001 cattgaaagt atcggtattg accgtgtgga aagcctgctc tccaccagcc cagattccaa
     3061 caatggcgaa gacagagaaa aaattgttgc ggaagaaatt gaagacagaa gcaataccaa
     3121 tctaaccatt acaaagacca ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa
     3181 tattgggatt tccatatttg tattcctatt aacaggaatc atgattttct cgcaggtact
     3241 ttttatcatc tatgctatgt ttctgcctgt gagctttatt ttaagcatga ttccatcatt
     3301 tgatggtatg tcaaaacgag ccataacaaa gctctttaat accattttga cacgagctgg
     3361 aatcacattg attattacga cagcatttag tatttcaacc atgctctata ccttatcggc
     3421 tggttatccg ttctttttaa ttgcttttct acagattgtg acctttgcag gaatctactt
     3481 caagctgggc gatttaatga gtatgttttc tctacagagt aacgattctc aaagtgtggg
     3541 aagtcgtgtg atgagaaaac ctcgtatgct tatgcacgct cacatgcacc gtctacagcg
     3601 gaaacttgga cgttccatga ctactctagg ggctgggtct gccattgtta caggtaaaaa
     3661 aggacagtcg ggttcgggga gttctgcaag gacacaagca gatcactccc gaccagacgg
     3721 aaaggaaaaa tcaacacttg gaaaacgtat cggtcaaacc atcggtacag tagctgatac
     3781 caaagacaga atggtagaca ctgctagtgg tttgaaagaa caggttaaag atttgccgac
     3841 caatgcaaga tatgcagtat atcaaggaaa atccaaagta aaagagaatg tccgtgattt
     3901 aaccagtagt atttctcaaa ccaaagcgga cagagccagt ggacgcaagg aacagcagga
     3961 acaaaggcga aaaaccattg cgaagcgtcg ctctgaaatg gaacaggtca aacagaaaaa
     4021 acagcctgct tcttctgttc atgaaagacc gactacaaga caagaacaat atcatgatga
     4081 acagacctca aaacagtcta atattcagac ttcatataag gaatctcaac aagccaaaca
     4141 agagcgtcca gcagttaagt ccgatttttc aagtccaaaa gtggaacgcc aaggcaatac
     4201 cgttcaagaa aaaaccgttc aaaatccagc aacttcaacc actacagcag atagaacttc
     4261 acaacgtcca atcacaaaag aacgtccgtc tactgttcaa agagtaccac tacaaaatac
     4321 aagaagtaga ccaccaatca aaaccgccac cattaagaaa gtcggtaaga aaccatgaag
     4381 ttgaaaactt tagtgattgg tggttctgga ttattcttga tggtcttctc actgcttctg
     4441 tttgttgcca ttttattttc agatgaacag gacagcggaa tttccaatat tcattatgga
     4501 ggtgtgaatg tttccgcaga agtgctggct cataagccta tggtagaaaa atatgccaaa
     4561 gaatatggcg ttgaagaata tgtcaacata cttcttgcga ttatacaggt ggaatcgggc
     4621 ggtactgcgg aagatgttat gcagtcctcg gaatccctcg gtcttccacc taattcattg
     4681 agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgaattatt agccagtagc
     4741 gaaaggctca gtgtagattt agaatcggtt atccagtcct acaattatgg tggtggtttc
     4801 ttagggtatg tggctaatcg tggaaataaa tatacctttg aactggctca aagtttctca
     4861 aaagagtatt caggtggcga aaaagtgtct taccccaatc ccatagccat acctatcaat
     4921 gggggctggc gatacaacta tggcaatatg ttttatgtgc aactggtaac gcagtatctt
     4981 gtcacaacag agtttgatga tgatacggta caagccatca tggacgaagc actgaaatat
     5041 gagggctggc gatacgttta cggtggagct tccccgacta cttcttttga ttgtagcgga
     5101 ctgacacaat ggacgtatgg aaaagctgga attaacttac cacgaaccgc acaacagcaa
     5161 tatgatgtga cccagcatat cccactatcg gaagcacaag ctggcgattt ggttttcttt
     5221 cattctacct ataacgctgg ctcttatatt actcatgttg ggatatacct tggcaataac
     5281 cgtatgtttc atgcaggcga cccaatcggt tatgccgact taacaagccc ctactggcaa
     5341 cagcatttag tgggagcagg acgaatcaaa caatgagaaa ggaagattta atgatgaaat
     5401 ttagaaaaaa tcagaataaa gaaaaacaga taccaaagga aaagaaacct cgtgtctata
     5461 aggtcaatcc tcataaaaag gttgtgattg ccttgtgggt acttttaggg cttagtttca
     5521 gctttgcgat attcaagcac tttacagcta tagatactca tactattcac gaaacaacta
     5581 tcatagaaaa ggaatacgtt gatactcatc atgtagaaaa ttttgtagag aactttgcga
     5641 aagtctacta ttcatgggag caatccgata agtccattga taatcgaatg gaaagtctaa
     5701 aaggctatct gacagatgaa cttcaagctc tcaatgttga tacagtacgc aaagatattc
     5761 ctgtatcgtc ttctgtaaga ggatttcaga tatggacggt agagccaact ggcgacaatg
     5821 agtttaatgt aacctacagt gtagaccagc tcattacaga gggagaaaat acaaagaccg
     5881 tccactctgc ttatatagtg agtgtctatg tagatggttc tggaaatatg gtactggtta
     5941 agaatccgac cattaccaac atacctaaga aatcaagtta taaaccaaaa gccattgaaa
     6001 gtgaggggac ggttgattcc attacaacca atgaaatcaa tgagttttta acgacgttct
     6061 tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat gacgggatat
     6121 taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt cacaatcgta
     6181 aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag accaaagcaa
     6241 cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg aagattgtaa
     6301 aataacaaat attggtacat gattacagat actttgtaat catgtactct ttttgataaa
     6361 aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg actatttaaa
     6421 aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg atagcgggaa
     6481 caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac
     6541 ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat acctatggtt
     6601 atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc ccttttgggt
     6661 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
     6721 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
     6781 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
     6841 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
     6901 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
     6961 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
     7021 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
     7081 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
     7141 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
     7201 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
     7261 tcattaaaag cattggaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
     7321 ttctctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
     7381 attacgaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
     7441 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
     7501 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
     7561 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
     7621 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     7681 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     7741 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     7801 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     7861 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     7921 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     7981 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc accgcttcca
     8041 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     8101 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     8161 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     8221 gcagattttc gaatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     8281 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     8341 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     8401 gaggtcattc ttagtggaga aatccctgct cgatgtattc aagaatatcg tagtgattta
     8461 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     8521 accggtgaac ctgtttgcca gccccgtcgt ccaaatagta ggatagataa agtacgatat
     8581 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     8641 ataagatgaa atattcttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     8701 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     8761 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     8821 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     8881 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     8941 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     9001 ggatagttct ttgtctgtaa agctatccat gtatttctct atctgtaatc gtcgggtgct
     9061 ttttaccaag ttattagcag gtaagaaaaa ttcatcaacg gaaacatgaa gtaacgatac
     9121 aaggtcataa agaacttgta tgctggggtg ttgcccttta ttttcaatat tagttaagta
     9181 ccgtgggtca atttcaatca atgctcccac ttgttcacga gttaaacctc gtttcaatcg
     9241 agcttcttta atggctaaac caaaggctct aaaatcatat ttatcttctt ttttacgcat
     9301 agtagaccac ctctatacat tttattgttc ctactgaatt aaaaacaggt atagaaaaac
     9361 gtgttatatg gtttataggt ttatatttaa taaaaagcac tactaaacgc caataaaaaa
     9421 aaccgttata tggtagtgct atttacgctg ttaaaatatt gtatattact tccaaatggc
     9481 ggtttgttgg aggtcaacgt cgccatgaag tacatcatat acaataaatt tccttacatt
     9541 gggttcttgt caaaaaaagt cgtctatctg caatagataa gtacgtccac caatgtggtt
     9601 ttataaatca tatagataga ataacagaag catgtaaaca gagaaataaa tctgtttata
     9661 tgcttttttg gctattcaga acttttttac aaagtttatt tatcagtaat gcaacaaatc
     9721 cccctttcac attgggacta agagtgaaag gagataaacg agcaaggctc acttcctttc
     9781 ctagacagaa agggggtgag aaacatgaaa ccatcttctt ttcagaccac aatagaaaat
     9841 cagtttgact atatctgtaa acgtgctatg gaagacgagc gaaagaatta tatgctttat
     9901 ctttcaagga ttgcaaagcg tgaggtgtcc ttttcggatg ttggcgatta tcttgttagc
     9961 cagtttgcga caacagataa ctattcaact gactttcaga tttttacact caatgggtta
    10021 tcagtaggcg ttgaaaatga tttgttgagt gaagcattac gtgagttgcc agacaagaaa
    10081 cgtgaaattc tactgctgtt ttactttatg gacatgagcg attcagaaat tgcagacctg
    10141 ttgaaattga accgttctac tgtctatcgg catagaacca gtggactagc cttaattaaa
    10201 aagtttatgg aggaatttga agaatgaaaa cacaatatcc tatgattccc tttcctctca
    10261 ttgtaaaggc aacagatggc gataccgaag cgattaacca gattctacat cattacagag
    10321 ggtacataac gaagcgttcc ctacgactta tgaaagatga atatggcaat caaagtatgg
    10381 tcgttgatga agtcttacgt ggaagaatgg aaaccagact gattacaaag attttgtcat
    10441 ttgaaattaa gtaatatcct ctctcctttc gtggaagcgt gctaaaccat tccacgcttc
    10501 ccgaacaggg aggtttgtta ttccaccaaa gcatattgag ctttcaatgt gttttgatag
    10561 gctaacgagc cattgttctt tgaaaactga ataaaagtaa tcgaatacgt ttcgataaga
    10621 aaagagccaa cggaactaac cgccatgacc tatcttataa agatagcgag cgattcatgt
    10681 tagtgatccg agaagcaatc tttagcagga ttgcctgcaa cgacattctt atcgtgataa
    10741 tgatactccc atacagtcaa tagtccgagc gtgataaaac cgtcgcaggc aatgagtatg
    10801 gctacatgag aaccatgcag gggtggaact cccgtgagct ttgctaaagc tgttcgattg
    10861 ctggtaaaac aacttttatg aaatccaaat aagtgatttg gaaaggagga ttttatgaag
    10921 cagactgaca tttctatttg ggaacgttat accctaacca ttgaagaagc gtcaaaatat
    10981 tttcgtattg gcgaaaacaa gctacgacgc ttggcagagg aaaataaaaa tgcaaattgg
    11041 ctgattatga atggcaatcg tattcagatt aaacgaaaac aatttgaaaa aattatagat
    11101 acattggacg caatctagcg tcgccaaagg gtcttgtata tgataaaata gtattaagtc
    11161 gtatcaaggc tctttccata aaggaaagga gcaaatgcca tgtcagaaaa aagacgtgac
    11221 aataaaggtc gaatcttaaa tactggagag agccaacgaa aagacggaag atacttatac
    11281 aaatatatag attcatttgg agaaccgcaa tttgtttact cgtggaaact tgtggctaca
    11341 gaccgagtac cagcaggaaa gcgtgattgt atctcactta gagagaaaat cgcagagtta
    11401 cagaaagaca ttcatgatgg tattgatgtt gtaggaaaga aaatgacact ctgccagctt
    11461 tacgcaaaac agaacgctca aagaccaaag gttagaaaaa acactgaaac tggacgcaaa
    11521 tatcttatgg atattttgaa gaaagacaag ttaggtgtaa gaagtattga cagtattaag
    11581 ccatcagacg ctaaagaatg ggctattaga atgagtgaaa atggttatgc ttatcaaacc
    11641 atcaataact acaaacgttc tttaaaggct tcattctata ttgctataca agatgattgt
    11701 gttcggaaga atccatttga ctttcaactg aaagcagttc ttgatgatga tactgtccct
    11761 aagaccgtac taacagaaga acaggaagaa aaactgttag cctttgcaaa agctgataaa
    11821 acctacagca aaaattatga tgaaattctg atactcttaa aaacaggtct tcgtatttca
    11881 gagtttggtg gtttgacact tccagattta gattttgaga atcgtcttgt caatatagac
    11941 catcagctat tgagagatac tgaaattggg tactacattg aaacaccaaa gaccaaaagt
    12001 ggcgaacgtc aagttcctat ggttgaagaa gcctatcaag catttaagcg agtgttagcg
    12061 aatcgaaaga atgataagcg tgttgagatt gatggatata gtgatttcct ctttcttaat
    12121 agaaagaact atccaaaagt ggcaagtgat tacaacggca tgatgaaagg tcttgttaag
    12181 aaatacaata agtataacga ggataaattg ccacacatca ctccacatag tttgcgacat
    12241 acattctgta ccaactatgc aaatgcagga atgaatccaa aggcattaca gtacattatg
    12301 ggacatgcta atatagccat gacgctgaac tattacgcac atgcaacatt cgattctgca
    12361 atggcagaaa tgaaacgctt gaataaagag aagcaacagg agcgtcttgt tgcttagtag
    12421 tacaaatgaa tttactactt atttaccact tctgacagct aagacatgag gaaatatgca
    12481 aagaaacgtg aagtatcttc ctacagtaaa aatactcgaa agcacataga ataaggcttt
    12541 acgagcattt aagaaaatat aaaaagataa ttagaaattt
//
LOCUS       DAUCWU010000048        12280 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUCWU010000048 DAUCWU010000000
VERSION     DAUCWU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.048"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..572
                     /locus_tag="VGY24_001181"
     CDS             <1..572
                     /locus_tag="VGY24_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ4284161.1"
                     /translation="PRGDKGETGEQGDRGETGPQGPQGDTGPAGPQGPRGEQGLQGDR
                     GETGPQGPQGETGPQGPQGETGQDGKDGLPGKDGKDGQDGKDGQPDPKTPEVPQKPDT
                     APHTPKTPQIPGQSKDVTPAPQNPSNRGLNKPQTQGGNQLAKTPAAHDTHRQLPATGE
                     TTNPFFTAAAVAIMTTAGVVAVAKRQENN"
     gene            758..1195
                     /gene="msrB"
                     /locus_tag="VGY24_001182"
     CDS             758..1195
                     /gene="msrB"
                     /locus_tag="VGY24_001182"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ4284162.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDDFFEKGIYVDI
                     VSGEVLFASLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            1325..2344
                     /locus_tag="VGY24_001183"
     CDS             1325..2344
                     /locus_tag="VGY24_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ4284163.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIVSTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDAIHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSFGVS
                     ADSFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAIQL
                     SLGIW"
     gene            2551..2976
                     /locus_tag="VGY24_001184"
     CDS             2551..2976
                     /locus_tag="VGY24_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ4284164.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            2995..3486
                     /locus_tag="VGY24_001185"
     CDS             2995..3486
                     /locus_tag="VGY24_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ4284165.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3503..4312
                     /locus_tag="VGY24_001186"
     CDS             3503..4312
                     /locus_tag="VGY24_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ4284166.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4309..5136
                     /locus_tag="VGY24_001187"
     CDS             4309..5136
                     /locus_tag="VGY24_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ4284167.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5272..6921
                     /locus_tag="VGY24_001188"
     CDS             5272..6921
                     /locus_tag="VGY24_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ4284168.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDELGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            6925..7713
                     /locus_tag="VGY24_001189"
     CDS             6925..7713
                     /locus_tag="VGY24_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4284169.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLKTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7707..8753
                     /locus_tag="VGY24_001190"
     CDS             7707..8753
                     /locus_tag="VGY24_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4284170.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            8868..10265
                     /locus_tag="VGY24_001191"
     CDS             8868..10265
                     /locus_tag="VGY24_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ4284171.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQGLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSMPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYAQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10367..12163
                     /gene="uvrC"
                     /locus_tag="VGY24_001192"
     CDS             10367..12163
                     /gene="uvrC"
                     /locus_tag="VGY24_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ4284172.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
BASE COUNT         3798 a         2243 c         2534 g         3705 t
ORIGIN      
        1 gcccacgtgg tgacaagggt gaaactggtg aacaaggtga ccgcggtgaa accggcccgc
       61 aaggtcccca aggtgacaca ggcccagcag gtccacaggg cccacgtggt gagcaaggcc
      121 tgcaaggtga tcgcggtgaa accggcccgc aaggtcccca aggtgaaacc ggcccgcaag
      181 gtccccaagg tgaaaccggc caagacggca aagatggtct tccaggtaaa gacggcaagg
      241 acggccaaga tggcaaggac ggtcaaccgg atcctaaaac accagaggtc cctcaaaaac
      301 cagataccgc accacataca ccaaaaaccc ctcagatccc tggccaatca aaagacgtca
      361 cacctgctcc tcaaaaccct tctaatagag gtctaaacaa accacaaaca caaggtggta
      421 atcagctcgc aaaaacacca gcagctcacg acacacacag acaattgcca gcaacaggcg
      481 aaacaaccaa tccattcttt acagcagctg ctgtagctat catgacgacg gctggagttg
      541 tagctgttgc aaaacgtcaa gaaaacaact aatcctcttt ttgctaccta actgatggaa
      601 agtaaaactc agagagaacc tcaaggttct ctcttttttg aatcaagata gtagctctag
      661 gtataacgtc ttgtctgagg tgctgtgaac aaatcagtta ccgctcacct ctttttttag
      721 taaaatgtaa gcattaaaac atcaaaagga gttttccatg gaaacatcag atgagttaaa
      781 acaacgtatt ggtgatcttt cttacgaggt cacccagcat gcagcaacag aatctccctt
      841 tacaggagaa tatgatgatt tttttgagaa gggtatctat gtcgatatcg tcagtggtga
      901 ggtcttgttt gcatcactgg ataaatttaa ttctggctgt ggttggcctg ccttttcaaa
      961 acccattgaa aatcgtatgg tcacaaacca cgatgactct tcttatggga tgagacgagt
     1021 tgaggttaaa agccgtgaag caggatcgca tttggggcat gtctttagtg acggccccaa
     1081 agaagctgga ggcttacgtt actgtatcaa ttcagcagct cttaaattta ttccttatga
     1141 ccagatggaa aaagaaggct atgcccagtg gctcacattg tttgatgaga cataacaggg
     1201 tttcaaaatg actgaaaaag aagcgtcagt gattcccaca agcactggat tggtgaggcg
     1261 tacgggtcaa ttttacaagc ctttgtaaaa ggtctataat agggaatgaa aaaaggaggt
     1321 tagtatgtca actcacttaa gaaaacttcc agggctgtta ctttgcttat tgttagctct
     1381 tccagcctgg tatttagggc gcttgtttcc tattattgga gctcctgttt ttgctattct
     1441 tttaggaatg ttgttagcct tgttttatga acatcgtgac aagactaaag agggaattag
     1501 ttttacatcc aagtatattt tacaaacagc agttgttttg cttggttttg gattaaacct
     1561 aacccaagtt atggcagtgg gcatgcagtc tttaccgatt atcgtttcaa ctattgcgac
     1621 agctcttttg gtagcttatg gctcacagaa atggctgcgc ttagatgtca atacagccac
     1681 cttggtgggt gtagggtctt ccatttgtgg ggggtctgct attgcagcga cagctcctgt
     1741 cattaaggca aaggatgacg aggttgctaa ggcaatttca gtcatttttc tctttaatat
     1801 gttagcagct ttgctatttc caagtttagg acaattacta ggcttatcta atgaaggttt
     1861 tgctattttt gctgggacag ctgttaacga cacctcttcc gtgactgcaa cggcgacggc
     1921 ctgggatgcc attcaccatt ccaatacact agatggcgca accattgtca aattgactcg
     1981 caccttggct attcttccaa ttactttagg tttatccctt taccgagcga aaaaagagca
     2041 cgacatcgtt acagaagaaa actttagcct taggaagtca ttccctcgtt tcatcctctt
     2101 ctttttatta gcttccctca tcacgacatt gatgaccagc tttggtgttt ctgccgatag
     2161 tttccattcc ctaaaaacct tatcaaaatt ctttatcgtg atggctatgg cagctatcgg
     2221 tttaaataca aacctggtta aactgattaa gacgggcggt caggctatcc ttttaggagc
     2281 tatttgctgg gtagctatca cgcttgtcag tttagccata caattaagtt tgggcatttg
     2341 gtaatcagac acgagaaatc agtttgactg atttcttttt ttgtatctta tttgtgtaga
     2401 attgtctaca ttttaaagag aatagtgcat tttaaaaacg ttttcatttg ataaaataaa
     2461 cctatcgtta caccagttca gtaaagacaa ctggttgaga taagcaatta ccgtttactc
     2521 agcggttttt gttaatagag gaggatagga atgaaacgaa aatttctcat tgggagtcac
     2581 ggcagactag ccagtggttt gcaaagctct attgacattt tagcaggtat gggacaggca
     2641 cttgaaacca ttgatgctta tgttgatgat agtgactaca ctagccagat tgatgacttt
     2701 atcgctgggg tagcagcaga tgagcagggc cttattttta ctgatttgtt aggcggcagt
     2761 gtgaatcaga aaatggtaac cgctgtaatg aacagtggca aagacaatat ctttttaatc
     2821 acaaacagta acttagccac tttattgtcg ttggtgtttt taaaaccagg cgaagcgctt
     2881 actaaagatg aaattgtaac cgttatcaat gaatctcaag ttcagttagt ggatttggtc
     2941 cctgagacaa attcagaaga tgactttttt gattaatatc taggaggaag acaaatgatt
     3001 actcaaattc gtgttgacga ccgtttgatt cacgggcaag tggcagttgt atggaccaaa
     3061 gagttaaatg cgccactttt agtagtggct aatgatgaag ctgcaaaaaa tgaaatcaca
     3121 caaatgacgc ttaaaatggc tgtgccaaac ggcatgaaac tgcttattcg ttcagttgaa
     3181 gactcgatta agctctttaa tgatccacgc gcaactgaca agcgtatttt tgtgattgtc
     3241 aattctgtta aagatgcttg tgccattgct aaagaagttc cagacttaga agctgttaat
     3301 gtggctaatg tggggcgctt tgataagtca gatccagcaa gcaaggttaa ggtgacgcca
     3361 agtcttttat taaacccaga agaaatggca gccgcaaagg aattagtcag tttaccagaa
     3421 cttgatgtct ttaaccaagt cttaccatca aataccaagg tccatttgag ccaattagtc
     3481 aactaaagag aggtaaaaaa gtatgttagt accagcaaca atggcagctt tagctgtatt
     3541 gatttgtttt gggggaaatt atttaaccgg tcaaagcatg atggaaagac ccctagtagt
     3601 tggactggtc acaggtcttc ttttaggaga tatgaaggtc ggtatcttaa tgggggcctc
     3661 tctagaagct cttttcctag gaaatgtgaa tattgggggt gttattgcag ctgagcctgt
     3721 aacagcgact gccatggcaa caacctttac cattatttca catattgacc aaaaagcagc
     3781 catgacccta gctgttccaa ttggaatgtt agcagccttt gtggttatgt ttttgaaaaa
     3841 cgtcttcatg aatatctttg ctccaatggt tgataaagct gcagcagcta accatcaagg
     3901 taaactagtg atgctccact atggcacttg gattatctat tacttaatca ttgcctctat
     3961 ttctttcatt ggtattttag taggtagtgg acctgttaat gcctttgtgg aacacattcc
     4021 tcaaaatctc atgaatggct tgagtgctgc gggaggcctc cttccagctg tcggttttgc
     4081 catgctgatg aaattattgt ggaccaacaa attagctgtt ttttacctat tgggctttgt
     4141 cttaacagct tatttgaaat taccagcggt tgcagttgct gccctaggag cagttatttg
     4201 cgtcattagt tcccaacgtg atctagaatt agacgcaatt actagaggtg caattagtaa
     4261 acaaacaacc tttgactcta aagaatcaga agaggaggac ttcttcgcat gacatcacaa
     4321 gataatttga ctaaagaaga ccgtaaaatg cttcgctcag tcttttggcg ctcatggacg
     4381 atgaatgcca gtcgtactgg ggcaacacaa taccacgcag taggggttat ttacacttta
     4441 ctaccagtca tcaatcgttt ttataagact gataaggata aagcagaggc actggtccgt
     4501 catacaacat ggttcaatgc caccatgcac atcaataact ttatcatggg tcttgtggca
     4561 tcgatggaaa agaaaaacag tgaagatcct gattttgatg ctagtgctat tacggcggta
     4621 aaagcatctc taatgggacc tatttccggc gttggagatt ccttcttctg gggaatttta
     4681 agggttattg cggcaggtat cgggatttcc ttggcaagtg caggctcagc catgggagct
     4741 gttgtcttct tactcttgta caatattcct gcttttatta ttcactacta tagcctttat
     4801 ggtggttatt ctgtaggtgc aggatttatc aaaaaacttt acgaatctgg tggtataaaa
     4861 atcgtcacta aaacctcaag tatgcttggg ttaatgatgg taggctcaat gacggcttca
     4921 aatgtaaaat ttaaaacgat tcttacagtt gctgcgaaag gtgccaaaga agcagcttct
     4981 attcaagatt accttgacca attgtttatt ggtattgtac cattaatggt aacactagca
     5041 gctttttggc ttctacgaaa gaaagtgaat atcaactgga ttatgtttgg tatcatgttc
     5101 cttggcatta tccttggact tttaggtatt tgctaataat ctcctagcaa taaaggtaag
     5161 taagacaagt tttcttgttt tgcttacctt tttcctttta agatgatgag acttgttttg
     5221 atgaaaaacg tagtagcatt aagaaatttg ttataatgaa aggagcttaa gatgcgaggt
     5281 gaacaggtgg aagaacattt taaaaaacaa ttacaagatg atatttctag acatttttcg
     5341 taccaatcgt taatgttatc attgttgcta attggtcttt ttattatttt ttcattagcg
     5401 ccacaacaat taggcctcta tcgtgatatc aatgccactg caactcgtta tcaccgtttg
     5461 attagcaaac aagaagcctt gctggatgaa ttagggaaaa atagcttact acctttttta
     5521 aataaaaacc tcagcactgc tgatttaagc aagcactatt ttcatttgcg ccatagcagc
     5581 caaacgtcac cagaactttt actattttca cctagtcaag gccttttatt tgctagtaac
     5641 ccacatttag gaaatgtttt tagtaaatct gtttatattc aagaagtctt gagagcagct
     5701 catcctccaa aaaccttgtt taaagttgct atggatagtg aagatggtca ctacttgatg
     5761 atcattaagc caatgattga tcaaaaccaa ctaaagggat atgctttttt agtgatgagt
     5821 ggcaaagatt ttcttcatcc tacaaaaaca ttgacgtcag agttggtcat tgctgataag
     5881 ctggacaaca cgtttacgtt ttccaatcgt gagtttatag catctagtct agataagatc
     5941 aatagccagt atttacacca ttattttgtt tttcaagata atcgagcctt tatcacaaga
     6001 aaggttgcct tacaaggagg tctttggctt tacatgtata ggcccttgat tccaatggta
     6061 tcggtgatgt tattttcact tatttcatca gctgttatct ttgtgatctt gcaacgtaaa
     6121 tcaagtggtt tagctaaccg aattgcagct aaaaattcaa gagcaatcaa tcaaatggtt
     6181 agagatatga gtgcgatctc tcggcaagaa aaaagacgta ttgaccttga gagtcaagat
     6241 gaatttcaat atttatctga tcaaatcaat caaatggtag agcgattgca acagttacat
     6301 gataaaacgt tagatttgga aactcaaaaa ttattatttg aaaaacggat gttagaagct
     6361 caattcaatc cgcatttcct ctacaatacg ctagaaacca ttttgattac gagccattat
     6421 gattctgccc taacagaaaa aattgttatc caattgacaa aattgttgcg gtatagtctc
     6481 acggattcta gcaaacctgt cttgctcaaa gatgatttaa gtgtcatcga gtcttattta
     6541 gtgattaatc aggtgcggtt tgaagaattg caatatagca ttaacctatc cccagacctt
     6601 gatagtcttg cagtgcctaa attgttcttg ttacctttga tagaaaatgc catcaaatat
     6661 ggcttgaaag aacgacacga tgtcaagatt aacattgctt gttattatca agatgaccac
     6721 attatttttt ctgtgcgaga taatggttcg ggaattgatg ctcatcacca aaaagtgatt
     6781 cgagagcaat tggaagctgg agagtcacac catgggttaa ttaactctta tcgtcggctt
     6841 aagtatcatt tttcagaagt atccttggtt tttgatcaag gtgataaaca gtttaatgtt
     6901 agctaccatg ttaaggagta gtctatgtac aaattagtca ttattgaaga tgaacatatt
     6961 attaggaaat ggttgcgata cgctattgat tataaagctt tagatatttt ggtcattggc
     7021 gaggctaaag atggcaaaga aggagctgtt ttaatcaaag agagtcaacc agatatcgtt
     7081 ttaaccgata ttaacatgcc catcatgaca gcttttgaca tgtttgaagc aacaaaagat
     7141 caaacttacg ccaaaattat tttatctggc tatgctgatt ttccaaatgc aaggtctgcc
     7201 atacactacg gtgtacttga atttttaaca aaaccgattg aaaaagcagc tctttgggaa
     7261 tgtcttaaaa ccattattgc taaaatagaa aagcaaaagg ggtctaatca aaaaacagat
     7321 gcttgcgtct atattccctt accccagatg actgatcaga ttcctgaagt agtcaaagat
     7381 attttggagt gggtacatgc tcattttcaa gacaagatca gcacgagccg cctagctcat
     7441 gacttagggt atagtgagag ttatatctat caaaatatca aaaagcatct tcagatgcct
     7501 ttaagtgatt acattaatca atatcggatt aatcaggcaa ttcaattgat gcaacaagaa
     7561 ccagatttaa tggtatatga aatagcacaa gctgtaggta tttatgacta tcgctatttt
     7621 gatagggttt ttaaaaagta tctaggacaa actgtaaagg cttttaaaga agagcatttt
     7681 atgaaagata ccgataggag taagccatgt taaaactcaa acgcaaatgg ctattaagct
     7741 ttttgttagt aataattatt ctagcaatcg ttaacgttgc aatgtatata ttctcatcgt
     7801 caaaaaaaga tagtgctaaa gaattagtta tcttgactcc taatagtcaa actattttaa
     7861 cagggactat tccagccttt gaggaaaagt atggggttaa agtaagatta atccaaggtg
     7921 ggacgggcca acttattgat caattaggtc gaaaagataa gccattaaac gctgatattt
     7981 tctttggtgg caattacact caatttgaaa gccataaaga tttatttgaa tcctatgttt
     8041 ctccgcaggt ttctactgtc atttcagatt accaattgcc tagtcatcgt gcaaccccat
     8101 atacgatcaa tggcagtgta ctgattgtta ataacgaatt agcaagagga cttcatatta
     8161 ccagttatga ggatttgcta caaccagctt taaaaggcaa aattgctttt gctgacccaa
     8221 atagctcatc tagcgctttt tcacaattaa caaacatctt actggctaaa ggaggctata
     8281 cgagctcaga agcttgggag tatgtgaaac gtttattact gtctatgaat tctatcagag
     8341 caacaagttc ttcagatgtt tacgaatctg ttgcagaagg taaaatgatt gttggtttga
     8401 cctatgaaga tccttgcatc aatctgcaaa aaagcggtgc aaacgtttcg attgtttatc
     8461 cggatgaggg gacagtattt gttccgtcgt cagtcgctat tatcaaaaaa gcaaaggcaa
     8521 tgaaagaagc taagcaattt attaatttta ttttatcatc agatgtacaa aatgcctttg
     8581 ggcagtcaac tagtaataga cctatccgac gagatgctca aacgagtcat gaaatgaaac
     8641 cattagatga aattattact ttaaaagaag attacggata tgttactaga cataagaaaa
     8701 aaatattgaa tacctataat cgactacatg aagcattaga aagaggaaag tagactctag
     8761 gcttgttgtt gctatactag tagtgttaca aaatagcgta gtcatttttt gaaatcgatt
     8821 tcataatctg gtagactaag gttatcacga aaaggaggct atatgtgatg gcttatcaaa
     8881 ctatttaccc ttatacaaat gaagtccttc atacatttga taatatgacc gatcaaggtt
     8941 tggcagatgt ccttgaaaga gctcaccttc tctataaaaa atggcgcaag gaagatcatt
     9001 tagaagaacg taaagcccag cttcatcaag tggctaatat tcttcgccgt gaccgcgata
     9061 aatacgctga aatcatgacc aaagacatgg gaaaattgtt cacagaagcc caaggtgaag
     9121 tagatctttg tgccgatatt gctgattact acgctgataa ggcagatgaa ttcttgatgt
     9181 ctatgccact agaaacggat tcaggtcaag cttactattt gaaacaatca actggtgtta
     9241 tcctagctgt tgagccttgg aattttcctt actatcaaat catgcgggtc tttgcgccaa
     9301 actttattgt tggaaaccca atggttttaa aacacgcgtc catttgccca cgctctgccc
     9361 aatcctttga agaattagtt cttgaagctg gtgctgaagc aggtagtatt accaatttgt
     9421 ttatttctta tgcccaagta tcacaagtga ttgctgataa acgtgtggtt ggtgtctgct
     9481 tgacaggttc tgaacgtgga ggagctagca ttgcagaaga agctggtaaa aacttgaaaa
     9541 aaacaaccct tgaattgggt ggcgatgatg cctttattat ccttgatgac gcggattggg
     9601 atcaattaga gaaggtgctt tacttctctc gtctctacaa tgcaggacaa gtatgtacct
     9661 catcgaaacg ttttatcgtg cttgacaaag actatgaccg tttcaaagag ttattaacaa
     9721 aagtcttcaa aacagctaaa tggggtgacc caatggatcc cgaaacgacg ttggcgccat
     9781 tatcatcagc tcaagctaaa gcagatgttc ttgaccaaat taagttggcc ttggatcatg
     9841 gtgcggagtt ggtttatggt ggtgaagcga ttgatcatcc aggtcatttt gtcatgccaa
     9901 ccattattgc tggcctcaca aaagacaacc ctatttacta tcaagaaatc tttggcccag
     9961 tcggtgaaat ctacaaagtg tcttctgaag aagaagcgat tgaagtggcg aatgattcta
    10021 actatggcct tggaggtact atctttagta gcaatcaaga acatgcgaaa gccgtagctg
    10081 ctaaaattga aactggtatg tcttttatca actcaggctg gacaagtctt ccggaactcc
    10141 catttggtgg gattaaacat tcaggctatg gccgtgaatt gagtgagctt ggctttacat
    10201 cttttgtgaa tgaacacctt atctatattc cgaataaaac gaataatagc aatacgaaag
    10261 tttgatctag attgttaaag gagatctgct cgccttaata aacaatcaag gcgttacact
    10321 cttctttttt aaagtcaaaa ttttaaaaat ttgataaact agtaacatga acgaactgat
    10381 taaacataaa ctagaactct taccagacag ccctggctgt tacttacata aagataaaga
    10441 aggaactatt atctatgtcg gtaaggccaa gaatttgaaa aaacgcgtcc gatcctattt
    10501 tcgtgggagc catgatacca aaacagagtt attagtatct gagattgtag atttcgaata
    10561 tattgtaaca gagtcagata ccgaagcgct tttgttggaa attaacttga ttcaaaaaaa
    10621 tatgcctaag tacaacatca agttaaaaga tgataaatct tatccctttc tcaaaatcac
    10681 aaatgagtct tttcctcggt tagtgattac aagatatatt aagaaaaatg atggattgta
    10741 ctttgggccc tacccagatt cttacactgc taatgaagtt aaaaagttat tagataggat
    10801 ttttccattt aaaaagtgta aaaatcctat caataaggtc tgtttttatt accatttagg
    10861 ccagtgctgt gctcatacca tttgtcatac agacaaggcc tattgggatc gtttaattga
    10921 tgatgttaag cactttttaa atggtaaaga tgacaaaatt attgaagatc ttcgttctaa
    10981 gatgttagca gcttctgaag aaatggcatt tgaacgtgcc gctgaatatc gagatttgat
    11041 ttcaggtatt gccaccatgc gcaccaagca gcgggtgatg agcaaggatt tgcaagatag
    11101 agatatcttt ggctattatg ttgataaggg atggatgtgt gtccaagttt tctttgtgcg
    11161 tcaagggaaa ttaattcagc gtgatgttaa cctttttccc tattataacg atgcagaaga
    11221 agatttctta acttatatgg ggcaatttta ccaagataaa cagcatttca tccctaaaga
    11281 agtctttatt ccagaagcta ttgatgaaga actggtagcc gctattgtgc caaccaaaat
    11341 catcaaaccc aaacgcggtg aaaaaaaaca actggtagct ttggctacta agaatgctcg
    11401 tgttagtctg caacaaaaat ttgatttatt agaaaaagat atcaaaaaaa cgagtggtgc
    11461 tatcgaaaat ttaggtcagt tacttaggat tgacaagcct gtgcgcattg aagcctttga
    11521 taattctaac attcaaggga caagtccagt tgcggctatg gttgtttttg ttgatggcaa
    11581 acctagtaag aaagattatc gtaaatttaa gattaaaaca gtggtgggac cagatgatta
    11641 tgccagtatg agagaagtgc tttttcgaag gtacagtcgg gtgaaaaagg agggactgca
    11701 agctcctaac ttgattattg ttgatggagg cgttggacaa gtcaatgtgg ccaaagatgt
    11761 gatagaaaaa caacttggac ttactattcc tgttgcggga cttcaaaaaa acgataaaca
    11821 ccagacccat gatttgcttt ttggaaaccc gcttgaagtt gtaccgttgc cgcgccgttc
    11881 tgaagagttt ttcttactgc atcggattca agacgaagtg catcgctttg cagttacatt
    11941 tcatagacaa gtgcgacgta aaaattcttt ttcatcaaca ttagatcata tttcagggct
    12001 tggcccaaag cgaaaacagt tattactaag acactttaaa accataacag cgattgcatc
    12061 agctacctct gaggagattc aagctttagg tatacctaaa acagtagttg aagcaataca
    12121 gcaacagata acagacaata aaaatgatag atcgtctcct taaataagtg ttatgttttc
    12181 agtactgatg aggtaaaaag gcgctacttg ttacgcacga catttcagtt cgttattttg
    12241 aaactttcag aaagttgcga agtgtagtga ttttatgatt
//
LOCUS       DAUCWU010000049        11691 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUCWU010000049 DAUCWU010000000
VERSION     DAUCWU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.049"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(68..1168)
                     /locus_tag="VGY24_001193"
     CDS             complement(68..1168)
                     /locus_tag="VGY24_001193"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ4284173.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSSSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLSKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(1165..1521)
                     /locus_tag="VGY24_001194"
     CDS             complement(1165..1521)
                     /locus_tag="VGY24_001194"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ4284174.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(1637..4156)
                     /gene="secA"
                     /locus_tag="VGY24_001195"
     CDS             complement(1637..4156)
                     /gene="secA"
                     /locus_tag="VGY24_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ4284175.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVGDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            complement(4290..5243)
                     /gene="manA"
                     /locus_tag="VGY24_001196"
     CDS             complement(4290..5243)
                     /gene="manA"
                     /locus_tag="VGY24_001196"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ4284176.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            complement(5338..6222)
                     /locus_tag="VGY24_001197"
     CDS             complement(5338..6222)
                     /locus_tag="VGY24_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ4284177.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIART
                     IAYFKAFEADLAGIAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     LHPDDMAKGFLGVCPFHKGCLEGMSAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            complement(6486..9473)
                     /locus_tag="VGY24_001198"
     CDS             complement(6486..9473)
                     /locus_tag="VGY24_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ4284178.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEGKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKGKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGVDKSRLF
                     IMDSTYMADKNPLIERGASYINLLLVQVYGSQGEKGVFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEENAQEGNLWYDINSRKDEDTANGINTDITGTRAERYARWQPKT
                     GGAKGGIFSYAIDRDGVAHQPKKYAKQKEFKDATDNIFHSDYSVSKALKTVMLKDKSY
                     DLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNKFKKLAQLD
                     LIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLKVSGLTGLK
                     ELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLSTVSNHVGS
                     NKQTVKFDKQKPTGHYPDTYGKTSLRLPVANEKVDLQSQLLFGTVTNQGTLINSEADY
                     KAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATDKEETYKVD
                     FFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADKEMSRNVFDGSLGGDRSN
                     LLGMSGPKSTIIFNLKESGIVKHWRFFNDRARSPQNPAEDIQEIKLQVFDSEKYDIET
                     LLKTPNQFDKDDYWITVDSYKSKSETVNNYDQVLKMNSAKYWRVTVDTKGGNYSWPSL
                     PELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALETSLNSKIFD
                     VTAINANAGVLKDCIEKRQLLKK"
     gene            complement(9704..11587)
                     /locus_tag="VGY24_001199"
     CDS             complement(9704..11587)
                     /locus_tag="VGY24_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ4284179.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVYDERKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGVIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLLAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
BASE COUNT         3386 a         2617 c         2070 g         3618 t
ORIGIN      
        1 ttctaatgtc ctcactttat tataacatta aagataagca caatgcaatt atttttacgt
       61 gattttctta atatatccga ggaatacggt cacttaaaag gcatagaact tcataattga
      121 ttgtattacg gtaatttgcg atatctgttg tagaaatatt tttttgctga ttgctgccaa
      181 tcaaagtgac ttttgttcct aaaggatatg ctttggaaag tcgtatggtc agttgatcca
      241 tcgatacacg ccctataatt tcacagaatt gtccatcaac taacaccgaa aagccttgca
      301 tgttcctggt ccagccatct gcataaccga ttgggacggt ccctacatat tcagactttt
      361 tggcagtata agtagctcca taaccgactg tatcaccagc tgaaatcatc ttgacatgca
      421 ctaagctaga ttctagagat aaagcctctt gcagtggaaa tggtaagctt agatcagaac
      481 tacttggatt caaaccataa ctgacaattc ctaaacgaac agcattaaaa atggtatcac
      541 tatgccagat acttgtggct gaattactag catgtactaa acgaggctta tcctcaagtc
      601 cagctatcag ctttttaaaa aactgtaact gctggttaaa tttagtatca tctgcctcat
      661 cagctgtagc aaaatgagta aaaatacctt ctacatcagc acccaactct tttagactat
      721 caatcaataa attgacttct tttgaagaac gaagcccgat ccgccccata ccagaatcaa
      781 cttttacatg aacttttaag ccttgacatt cttttttctc cagtctagct aaagctatcc
      841 agtctaaact agcgattgta acagtaatag cattagcaac tgctaactct aattcatttg
      901 gcaggaggac cccaagaatt aaaatctctt tatcaatacc tgcttgacgt aattgcaaag
      961 cctcatcaag atttgacaca cagtacccat ccacttgagg taggagtgct tttgacacct
     1021 ggacagcacc atgaccataa gcatcagcct tgacaactgc atacgttttt acccctagtg
     1081 gaatatgctt ttgaacactg gcaacattct ccttaatagc ttgtaaattg acccttgcaa
     1141 ctgttgggcg atggaaactt gaaatcattt tttatcctcc aaaataacac tagcctgtac
     1201 ataattgcca ctatgtgaga tgctaataaa actatttcct ttaaacggag attttgtcag
     1261 aatgggacat cctctaacat catttaaaat ctcaatatct tgaaaggtta accgcccaat
     1321 acctgtccct atagccttag caaaagcttc cttacctgac cagcgtcctg ccaaatagct
     1381 gagtcgacgt ttgtaaggaa aactctcaaa aatagccaat tcctgctcag ttaaaatttt
     1441 ttgagcaaag cgaggatttc tttgataaac tttttcaata gctgaaatct cttgtaagtc
     1501 tattccatgt ccaacaatca ttactttcca ccaaacctta tttcatctta atgttagaag
     1561 atttaaacag tgagaagctg agaattattc tcagcttctt tctatatacc taactttgtc
     1621 ctgttgtgtc tcgttcttat gagaaggatt tacgaccgtg acaatttttg aattttttgc
     1681 ctgaaccaca tggacaagct tcgttacgct ccactttggg taaatcatcg gtgttagctg
     1741 actgctgcga ttggatgttt tgaggtgctg ctgttgtggc acgttgactt gcacgttcac
     1801 gttcttgttc gtgaatttgt gctttcatca tggtacgtgt tacatcaaac tcgatagcac
     1861 caatcatatc ttggaacatc ttgaaacctt ctgcttggta ttccaccaca gggttatttt
     1921 gagcataccc ccgaagtcca acagcattac gaagttgatc taaggcatca atgtgttcag
     1981 tccatttgtt atccacaatc atcaaaatga gtactttttg gaattcaata atagcttctt
     2041 gatcacgaag ttttgatagt tgctggtcgt agatagcaag cgctctttga taaagcttct
     2101 ctttgatttg atcatctttt aaaccacgta actctttagc actaatagac tcttctggta
     2161 ccagcgaggt cctagcaaaa gttacaatag catctatagc atctttacga ttgctacgtg
     2221 catgagcatc tacagcacgg tcgatagtac gcttgatcat agctttaatt tctgggccaa
     2281 gatctcgatt agcagttatg acatcacgac ggttagcata gataatttca cgctgttcac
     2341 gcatcacatc atcatattgt aagacctgtt tacgggtatc gtagttattt ccttcaaccc
     2401 gtttttgagc agattccact tgacgcccta acatacctga tttaataaca gtgtcttctt
     2461 catctaattt catccgatcc aaaaaggctt ttatacgatc agagccgaaa cgtctcatca
     2521 aatcatcttc taatgaaaga tagaattggg attcacctgg atctccttga cgacctgaac
     2581 gtccacgaag ctggttatcg atacgacggc tttcatgacg ctctgtacca atgacacata
     2641 acccaccaag ttcacgaaca ccttcaccaa gcttgatatc agtaccacga ccagccatgt
     2701 tagttgcgat tgtaacagca ccacgttgcc cagcattcat aatgatttga gcttctttaa
     2761 agtgattttt agcattcaaa acctcatgag gaatccctgc ttcaaccagc ttgcgtgaaa
     2821 tcaaatcact agtttcaaca gcaaccgtac ctacaaggat aggttgtcct tttgcatgac
     2881 gcgttttaac gtctcccaca actgctctaa actttgattc taaggtcggg taaagcaaat
     2941 cagtgtgatc aatacgggca attggacgat tggttggaat tggaataata cgcatgttat
     3001 aaacttcgcg aaattcttct tcctcggtct tcgcagtacc agtcatacct gccaattttt
     3061 tgtacatacg aaacatattt tggtaagtga ttgaggcgct ggtttttgat tcttcctgaa
     3121 tacgaacgcc ttcttttgcc tcaatagctt ggtgcaagcc gtctgagaag cgacgccctt
     3181 ccatcgtacg acctgtaaat tggtcaacaa taagaatctc accatcttcg ctgaccacat
     3241 aatcgatatc taacaacata atatagtttg cacgaagggc gttgtctata aagtgagtca
     3301 aggctacatt ttcaatatcg taaagattgc tcaagttaaa gtaactctca gctttatcaa
     3361 tacctgaatc gcttaatcca atcgttttag tgggcacatc aataacataa tccactgacg
     3421 tcagagtttt tacaaacatg tcagcacgaa tatacaattg attggtttca gagctaacag
     3481 caccagaaac aattaaaggt gtccttgcct cgtcaattaa gactgagtcc acttcatcaa
     3541 ccaaggcaaa attgagtggg cgttgtacca tgtcttcctg gcgaacaacc atgttgtcac
     3601 gaaggtaatc aaaaccgact tcagagttgg ttgaataagt aatatcacaa ttataagctt
     3661 cacgtttttc agcaggagac ttagcagcta ggttgatccc aactgaaaga cctaaccagc
     3721 tatacacttc acccatttcg gtagcgtcac gcgttgaaag atactcatta acagtaataa
     3781 cgtgaacacc ctcaccagca atagcattga gataaactgg catggtggct gtaagagttt
     3841 ttccttcacc agtacgcatt tcagggacat ccccgttatg aagaacaatt cctcccataa
     3901 tttgaacacg ataagggaac aagcccaaca cacgttttgc tgcttcacga acaactgcga
     3961 aagcttcagg taatagttgt tcgagggtct cacctttttg atagcgttct ttaaattcaa
     4021 gtgtttttcc ttgtagatct ctatccgaca atgacgccat ttggtctgcg taggattcta
     4081 ctttttttgc aattttttct aattttctta gttcgccttt gtcattttcg ataactttgc
     4141 gtagaatatt ggccattctc tttcctttcg atcatctaat catttcattt taacataaat
     4201 atgcaggcag ttcaagaaag gttggtaaag ttatcagaga aacagagagt cataatttta
     4261 tccttttttc tcatattgat accatggtat taaacgagtt gattggggtg actcacaatc
     4321 atttctaatt gaccatcaaa tgaccacgat ttaacatcgt taggcaagat aaagtgcatg
     4381 cctttttcta gctcataagc cttctggtcc acatagagtt ttccttgacc ttttaaaaca
     4441 ctcactaaaa gataaggcgc agcttgtttc atgtcaacca tttgactagt tacccatttg
     4501 taaacagtga aaaatggcgt tgagactaaa gttgttgcta ccatattatc caaaaccata
     4561 gtggctggca cgctattttc tggcttacca atcgttaaga catcaataga cttttcaata
     4621 tgaaggtcac gaagattacc attaacgtcc ttacggtcaa aatcataaac ccgataagtg
     4681 gtatcagatg actgctgggt ttctaaaata agaattccct tgccgatcgc atgcatggtg
     4741 ccacttggaa catagaaaaa atcgcctgct ttgactggga cacgagtcag caaatcatcc
     4801 caagcgcctg cttcaatcat ggcacgaagg tcttctttgg acttggcctg atggccgtat
     4861 acaatttcag aaccctcttc ggcagaaata atataccagc attctgtctt gcctaattct
     4921 ccttcatgtt cacgtccata agcatcatca ggatgaactt ggacacttag ccagtcatta
     4981 gcatctaaaa tcttagttaa cagtggaaaa acttcttcct ttggattccc aaacaaggcc
     5041 ggctcttgcg catataatgt gtttaaaggt tgtccttgat aacgtccatt agtaactgtc
     5101 gaaacaccgt taggatgagc agaaatagcc caatactcac ctgtggtgtc acttgggatg
     5161 ttgtaagcaa agacatctct caacttggtg ccaccccaaa tcctgtcgtg catggttgat
     5221 tttagaaata atggttctga cataatattc ctcgcttctg atgttgatct ctgtttatta
     5281 tagcatgttc tagctgactg cgctttcttt tactagacaa gcaggcaaaa acagacccta
     5341 tttcccttgt gcagccaact tggccaaagc aaaatttccc agagtagcgg agccattatc
     5401 agcaacagct ggagtaacaa tgtagtctgt caaatcaggg actggcaaat aaccgcttaa
     5461 aagggtagtg aatttatcat gcacgcgcaa caccatatgt tcttgcgcca tgacacctcc
     5521 gccaaaaaca atcacttgag gacggtaaag catagttgct tgcagcgctg cttgggcaat
     5581 gtagaaggct tgaatgtccc aaacatcagc ttcctgatca agtcgttcac ctctcactcc
     5641 cgttctggcc tcaatacttg gacctgctga catcccttcc agacaccctt tatgaaaagg
     5701 gcaaacacct aaaaatccct tggccatatc atcaggatgg agcatgacat aggtatgacc
     5761 cgcttctgta tgacctaatc ccccaataaa gtggccatgt tgaatagctc ccgctccaat
     5821 accagtacct atggtgtaat agaccaaact ttccacacca ggacgagcaa gcacctcacc
     5881 ataagcagag ctattaacat ctgttgtcac atcaaaagga atcttaaaag cagccgaaag
     5941 ttgtcctaac aaatccacat tagcccagcc tgacttagga gtggttgtga tgtaaccgta
     6001 agtctctgac gacggatcaa tatcaattgg tccaaaagaa ccaatagcaa taccagccaa
     6061 atctgcctca aaagctttaa aataagcgat ggttcgcgcg atggtttctt ctggtgtggt
     6121 ggtcggaaat tgtgttttat ccaccactgt aaactcctca tcacccacgg cacagacaaa
     6181 ctttgtccca ccagcttcaa tgcttccata taattttccc atgttttctc ctttttcttg
     6241 ttctttttta tctttatgat gacacaggct cttattaaga cagccatgtc tcacggacaa
     6301 gcgccacttt ctggtagtct aacgattatt tagctttatg tcatcaaaac ctataaaaga
     6361 ctaaaaatct gggactattc atctcagatt tttagtcttt tggttaatca attccagcaa
     6421 acctttatca tagtcacatt ttgttaattt aatctgaatg ttgctatcta agagagttac
     6481 tttgtttatt tttttagcag ctgccttttt tcaatacaat ctttcaaaac tccagcatta
     6541 gcattaatag cagttacatc aaaaatctta ctgttcaaag aagtttctaa agccatctct
     6601 tttattttta actcatcaag catcttttga gaaaacttat ctttttgttg agatagacct
     6661 ttagcaatag ttactgtttt catgatagtg tcggcgttag gtaacggata acctaaaatt
     6721 tggagttcag ggagtgatgg ccaactataa tttcctccct tagtatctac ggttactctc
     6781 cagtatttgg ctgaattcat tttcaaaact tgatcataat tattaacagt ttcggatttt
     6841 gatttataac tatcaaccgt aatccagtaa tcgtctttat caaattgatt aggcgtcttt
     6901 aatagtgttt caatatcgta tttttctgag tcaaagactt ggagtttaat ttcttggatg
     6961 tcttcggccg gattctgagg tgaccgtgct ctatcattaa aaaatctcca gtgttttact
     7021 attccagatt cttttaaatt aaaaataatt gtagattttg gtcctgacat gcctagtaga
     7081 ttagatcggt ctcctcctaa agaaccatca aatacgtttc tagacatttc cttatcagca
     7141 cttcctccaa taactgttgc gccttctgcc aaattaacca tcatggtttt ttcgtcacca
     7201 acaatcactt tagcagtatg aacagctttt gtcttatctg ctgggctaaa gaagtcaacc
     7261 ttataggtct cttctttatc agttgctagc gttttgtcag tagtggttcc caatgtggaa
     7321 tcagttactt taacggtata gttctcataa gaaactttaa agttattgta atgatagttt
     7381 gaatcaacaa agctacgtcc agcaatctgc tgattttggt aagccttata gtctgcttcg
     7441 ctattgatta gggttccttg attcgtcaca gtcccaaaca aaagctggct ttgcaaatca
     7501 actttttcat ttgccactgg taagcgcaga ctagttttcc cataggtatc tgggtaatgc
     7561 ccagttggtt tttgcttgtc aaatttcact gtttgtttat tgcttccaac atgattgctg
     7621 acagttgata gcatagtatc aaaaatttgt cgattttctg ttcctggagc caaatcaagt
     7681 ttgttgccag aaatatcaac tttttctaaa gacgttagag tagcggcatc aagaccagcc
     7741 aaggtttcac ggtcaaaacc tgacaaatct aattctttca gaccagttaa accagaaacc
     7801 ttcaaagata ctggtgggat agtagcaggt tcttctttgt tatccttttt ataggtttca
     7861 agaactgttt ccaaggtatc tttgcctggc ttcatattag cgggtaaaac agattggtcg
     7921 agctttgtaa tgcgagataa gccaatcaag tctaattgag ctaatttttt aaatttattt
     7981 agaccttcta aactttgaat cgctggatta tccaatcgta atgtgccatt gaaacgttcc
     8041 aaatcacctt ttctggttcc aacctgcgcc atcacagctt ctcgcaaagc cttatctggg
     8101 aaatctttct catcaatcag atcatacgac ttatctttta gcataactgt ctttaatgcc
     8161 ttggagacac tataatctga gtggaagatg ttatcagttg cgtccttaaa ctctttctgt
     8221 ttagcatatt ttttaggttg atgagctaca ccatctcggt caatagcgta ggagaagata
     8281 cctcccttag ccccacctgt cttaggttgc caccttgcat accgttcggc acgcgttcca
     8341 gttatgtcag tgttaattcc atttgccgtg tcctcgtcct tgcgagaatt aatatcatac
     8401 caaagattcc cttcttgagc attttcctca tagaaagaaa aaccaatcat gtattgttca
     8461 ggacgaatat acttgctata accttgccat cgttcttcag gggtatcggt gactaatttg
     8521 gtatcatttt gaaagacacc tttctctcct tgtgaaccat agacctgtac cagtaataaa
     8581 ttaatataag aagctcctcg ctcaatcaat gggtttttat cagccatata ggtgctatcc
     8641 ataataaata accgcgattt atcaacacct tttggtccaa ttaatttccc aatttcttca
     8701 aaaacatcaa tagatcgctt gagattttcg tcactagctt ctccatttac ttttggaata
     8761 ctatcatgtt caacatccac atctaagcca tcaaggttgt acttataaac atattcatca
     8821 acaatagctt tggctaaagc tttatttccc tctggtgtat ttgggtattt actggtatct
     8881 tctgcaatac cactgttatc acctccagct aggaaacgcc atggaatggt acgaatgaca
     8941 cgtgtccctt gcttgtttaa ctttggcaca tgtttggtgg ccaattcttt ccaaaaaagg
     9001 ctataatctt ttgtccaatc gtggaaaata aaggctagat ctacttcttt aggaagctct
     9061 cccatcgagt taactttgtc tttttctgtt ggatctgatg ttttgtcatg ccaagttcta
     9121 aagtaaccac cgtagagagg accatgtaac ggtttcatcg gtattttctc aggaattttc
     9181 attttggcaa gttcttgagc ttgtttatct gcctgctgag cttttgctaa tatctctttg
     9241 cccttttgag attcttgccc cgcttttgag agttcttctt taaattcttt tttgctattc
     9301 tctgacagat aatgcaagct atctatagaa gataattctt tctgaacctg aacagtcttc
     9361 ccctccgctt ttacagtatt gagtgaatca tggtgggtcg ctaaggcagc tcccatcaac
     9421 gttgcagcac aaacacaccc tagtgttctt tttaccaaca aatgtttatc catttggaca
     9481 ctccttattt ttggtactaa gtcatggctt cccagttgat ataccatcta tgtaagcgca
     9541 ttcctatagt tcgccttaag tataacaaca ctttattaaa aaaaacaagg tacaaagtat
     9601 aggtaaaata acacaaattt aaggttttta ggcttttctc atttttttgc caacctgcca
     9661 ttcatggcag gacaaaagag ctgcttagtt agctcttttg tccttatttg ataagtgcca
     9721 cttgatcacc aatcaaaaca tgtccttgag caagaatgtc cacgctttga tagtcagcgc
     9781 tattggtcac aatcatcatg gtggtgtcat ctagtccagc ttctgcaatc ttgctaggat
     9841 caaagtgtcc aagaagatca ccttttttaa ccgcctggcc tactgctaca agagattcaa
     9901 agccatctcc tgccatcgac acggtatcaa taccaatatg aagtaacact tctgctcctt
     9961 gacttgacgt tatagcatag gcatgacctg tttcaaagac aatttcaact ttaccatcaa
    10021 ctggtgagta tagggtatta tcttctggct taattgctaa gccttgaccc atagcacctg
    10081 atgaaaaaac agggtctgaa acagctgcta aatctacaac tgttccattc aaaggactat
    10141 ataaagtttc ctctgctaag gctggctgat cagccgtttg atcaacttcg acagctagta
    10201 acggaagggt ttctctatct tgataacccc aagtataagc aattgcaaag gctaccccaa
    10261 gaccgacaag catagtcaca aggtattgta ataattggcc attcaagtaa aggagagttc
    10321 ctggtaagac cgtaatccca aaaccagttc ctgcaatgcc aaagagccca gcgacccaac
    10381 cacctaaggc accaccaata agacctgaaa caaagacttt tggataacgg aggttgaccc
    10441 caaaaatagc tggttcagta atccctaaaa gagctgacaa ggttgatgga aaggccagac
    10501 cttttagttt tgttgatttg gttttaacag caacagctaa ggtagctcca gcttgagcag
    10561 ctgttgctgc tgttaggtaa gcattgaaag gatcttttcc ggtattggca attagctgcg
    10621 cttctaggaa gttaaagata tggtgaatac cagtcactac gattaattgt tggattaccc
    10681 caacaatcaa gcctgcaata ccaaacggca aatgcaagac ggcctgtgtc ccagccagaa
    10741 caaggttttc aagagaatgg aagactggtc caatcacaaa tagtcccaag atactcataa
    10801 tagcaaacgt taaaaacggt gtgaccaata agtctaaagc ttctggaacc tttttgtgca
    10861 accatttctc caacttagct cctaccagac caacaaagaa ggcaggtaaa acggtacctt
    10921 gataaccaac aacaggaaca aatccaaaga aggttagcgg cttaacatct ccaccagagg
    10981 caactaccca ggcgtttggc aattcattgg acaccagcat caaacctaaa acaataccga
    11041 taataggatt acccccaaag accctaaagg ctgaccaagc caccaaagct ggcaagtaga
    11101 caaaggctgt atccgttaga atacgagtgt acatgagaaa attttccccg tactcatgca
    11161 ccccaaataa atccataata gctggctggg tcaccaaacc acgaaccccc ataaagagac
    11221 ccgttgctac aatagctgga ataatgggaa caaagacatc tccaaacgta cgaatcgccc
    11281 gttggaagat attgccttgt ttgcctgctt ctgccttttg ctcactggtt gatgacgtgg
    11341 gtaaaccaag agcaacaact tcgtcataaa tgttattaac ggtaccagtt ccaaaaatca
    11401 tctgatattg accagaatta aagaaagctc ctttaacctt gtcaatagct tctgcttttt
    11461 ccttatcaat ctttctttca tcataaacca tcacgcgaag gcgagttgca cagtgggcaa
    11521 cacttctcac attttctcgg ccacctaaag cctcaatcac ttcagttgca atctgacgat
    11581 tatccatgcc taaaagtctc ctttttcatt tggtgttagt ccctcatcca ttctgccagc
    11641 tttgtggcta agaatgtgca ggaccaacgc tagtgaatcc gcttcacttc t
//
LOCUS       DAUCWU010000050        11598 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUCWU010000050 DAUCWU010000000
VERSION     DAUCWU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.050"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            171..419
                     /locus_tag="VGY24_001200"
     CDS             171..419
                     /locus_tag="VGY24_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ4284180.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            456..1067
                     /gene="amaP"
                     /locus_tag="VGY24_001201"
     CDS             456..1067
                     /gene="amaP"
                     /locus_tag="VGY24_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ4284181.1"
                     /translation="MLSRKEVCMSKLLKISYCLAGLVLLSVFGWVVGITGGYIYLPCS
                     YRWLSWGMDSFSNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGITEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1078..1266
                     /locus_tag="VGY24_001202"
     CDS             1078..1266
                     /locus_tag="VGY24_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ4284182.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1279..1818
                     /locus_tag="VGY24_001203"
     CDS             1279..1818
                     /locus_tag="VGY24_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4284183.1"
                     /translation="MTETYIKNTSKDLTSVIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1859..2059
                     /locus_tag="VGY24_001204"
     CDS             1859..2059
                     /locus_tag="VGY24_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ4284184.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGLGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            2070..2558
                     /locus_tag="VGY24_001205"
     CDS             2070..2558
                     /locus_tag="VGY24_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4284185.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2721..3362
                     /locus_tag="VGY24_001206"
     CDS             2721..3362
                     /locus_tag="VGY24_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4284186.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3505..4047)
                     /locus_tag="VGY24_001207"
     CDS             complement(3505..4047)
                     /locus_tag="VGY24_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4284187.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4165..4866
                     /locus_tag="VGY24_001208"
     CDS             4165..4866
                     /locus_tag="VGY24_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284188.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4881..7517
                     /locus_tag="VGY24_001209"
     CDS             4881..7517
                     /locus_tag="VGY24_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ4284189.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHHLI
                     MSDTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7609..8205
                     /locus_tag="VGY24_001210"
     CDS             7609..8205
                     /locus_tag="VGY24_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ4284190.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8216..9172
                     /locus_tag="VGY24_001211"
     CDS             8216..9172
                     /locus_tag="VGY24_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ4284191.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9269..10609
                     /locus_tag="VGY24_001212"
     CDS             9269..10609
                     /locus_tag="VGY24_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ4284192.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALVKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10725..>11598
                     /gene="truB"
                     /locus_tag="VGY24_001213"
     CDS             10725..>11598
                     /gene="truB"
                     /locus_tag="VGY24_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ4284193.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKK"
BASE COUNT         3709 a         1921 c         2414 g         3554 t
ORIGIN      
        1 taggattttg aaatatcggt attcaaacaa aaaataaact aacactttat tatttagttt
       61 atatagtcac tttttgaaag tcgactatta aaagactatt gcttgaagaa tgacagctgt
      121 tattcttcct tggacaaaag gtacaggagt atctaaaaag gagaattatc atgggactta
      181 tttggacttt aatcgtcggt gcgcttattg gtgtgattgc tggagcactt actaaaaaag
      241 gtggttcaat gggctggatt gcaaacatcg ctgctggtct agttggttct tccgttggtc
      301 aagctttact tggttcatgg ggcccttctt tagcaggaat gtctttgatc ccgtcagtta
      361 ttggtgccgt tatcgttgtt atgatcactt cttttgtact aaacaaaact aacaactagt
      421 ttcctcttta tttagtaagt acttacaaat agagaatgtt aagtagaaaa gaggtctgta
      481 tgtcaaaatt gttaaagatt agttattgtc ttgccggact tgttctattg tctgtttttg
      541 gatgggtagt tggaataacc ggaggctata tatatttacc ttgtagttat cgttggctaa
      601 gttggggtat ggatagtttt tccaatctct tagattcagc tttatcctat tattacttct
      661 ggacagccct tgtattattt gttataacat ttcttgcctt attagttatt attttatacc
      721 caagaattta tactgaggtc cagttacgcc ataaaaataa aaaaggaaca cttttattaa
      781 aaaaatcggc tattgaaagt tatgttgcga ccgctattca aactgcaggt ttgatgccaa
      841 acccaacagt cacagctaaa ttatataagc ggaagtttaa gattatcgtt aaaggacgtt
      901 tagcttctcg tgtagcagtg gcagatcaaa ttagtggcgt gaaagaaggg atcgaaaaag
      961 gtataaccga gttcttcgga attaattatc ctgttaattt caaagtttat gttaaagata
     1021 ttgctgattc tgatcgtaaa catattacca gaaatcgtgt agaataggag atctatcatg
     1081 gaattttatg aaaaattcaa gtaccctatt attggtggct tggtggggct aattattgcc
     1141 atattgctca tggcttttgg tttattcaaa acgctgctag caattatttt cattatttta
     1201 ggaatatatg gtggcctgta tgccaaaaaa acaggtatta ttgatcaatt tcttaatcgc
     1261 aaataaagga gacttattat gactgaaact tatattaaaa acacatctaa agacttaact
     1321 tcagtaattc gtggccaatt aacatatgat gataaagtaa ttgaaaaaat tgttggttta
     1381 gcacttgaaa atgttgatgg gttactgggt gtaaatggtg gtttttttgc taacctaaaa
     1441 gataaattag taaacaccga atctgtacgt gatggcgtga acgtggaagt tggaaaaaaa
     1501 caagttgccg ttgatcttga tattgtcgct gaatatcaaa agcatgtgcc gactatttat
     1561 gactctatta aatcgatcgt tgaagaagaa gtgaagagaa tgaccgatct ggatgtgatt
     1621 gaagttaatg ttaaagtagt cgacattaaa acaaaagaac aatttgaagc tgaaaaagta
     1681 agtttacaag acaaagtttc agacatggca cgttcaacat ctgaatttac gagtcatcaa
     1741 gttgagaatg ttaaagcttc agtggataac ggtgttgaaa aacttcaaga tcaaaaagct
     1801 gagccacgtg taaaataatc atatgtcgtc aattaggtaa gctaaaggag ggtttattgt
     1861 gtcagatgaa aaatataatg cgaaattaga tcaagctggt ggcaagttaa aagaaggatt
     1921 aggtaagatt tctggagata aatctttaga aactgaaggt aaagtagata aagttactgg
     1981 aaaagttaaa gaggttattg cagacgccaa agatactgta aaaggattag ctaaaggctt
     2041 agataataaa gataaataaa ggggacatga tggaaaaaac tcaaattaaa agcactctta
     2101 cttatgacga taaggtcatc gaaaaaattg tcggtcatgc tttggaaaac gttggcggat
     2161 tactagctgt aacaggtgga tttttctcaa atatcaaaaa taatttggtg aattctgagt
     2221 ctgtaactga tggtgtttca gttgaagttg gtagcaagga agtagcagtt gatttagcaa
     2281 tcattgtaga atacggtaaa gatattcctg ctattgcaga atctattaaa gctattgttt
     2341 ctcaaaacgt tgacagtatg acacatttga aagttgttga agtcaatgta aacgttgttg
     2401 atattcgtac taaggaagaa catgaagctg ctagcgttac tgttcaagat cgtgtcacta
     2461 gtgcagcttc aagtacttca caatttgtat cagaacaaac agaaaaatta aaagatacta
     2521 tctctgatac tgtaaatagt gatgaggcag ctaaataagc aatcgtctag gaagtattat
     2581 tgactggtgg tgataagttt gagctggtat ttaccagctc tttttgtcac ggttaaaaag
     2641 atacggacta ttgtcatctc tctaaaaata tgatttaatt agatataaat ttttcaaatt
     2701 gttagaaagg agtaaattaa gtgataagtc ctaaaaaaga gataacaagt tcaaaatatc
     2761 aaaaaattgc tatttcagta gctcagcgta tcgcaaatgg cgaatatgaa gtgggcgaaa
     2821 agttaaagtc aagaactact atcgcctcga catttaatgt atcaccagaa acggctcgta
     2881 aaggcctgaa tattttagct gacttgaaaa ttttgacttt aaaacatggt agtggagcta
     2941 ttgtgctgtc aaaagaaaga gctattgagt ttataaatca atacgaatca acccattcca
     3001 tcgctgtttt aaaagaaaaa atacgtgaga ctattaatga tcagggaaaa gcaatggaaa
     3061 aaatggctgt tttggtcaat gattttttaa tgcaaagtca atctgttagt aagcaatacc
     3121 cattagcacc ttatgagatt atttgtagtc aagattcgga acattttggc aaatctatcg
     3181 gtgtgttaaa tatctggcac cagacaggag caactatcgt cgctattgaa catgctggtc
     3241 agtttatcgt ttcaccaggt ccttattctg ttattgaaaa aggcgatcat atttatttcg
     3301 ttggtgatga gtcagtcatt tcacgaatga agaccttttt taatttacga aaaggattat
     3361 aagtttgagg gcccacctca aacttttttt gttgctgttg agcattatta aaagaggcag
     3421 atcatcatgg tttgtctttc caaaatatct gtttagacag ttgttttgga ttgggagcat
     3481 gatttcagac tcttttgatg ttttttaatt gtctagagta actgctttga gaataatatc
     3541 ggcaacctct tcagtggttt cttgatagcc attagcaatc caatagctaa taatgctctc
     3601 aatactggca aggtaaactt ctaaagcata gggataagga atatggtatt gattggtcac
     3661 aatgtcttgg aaaccagtaa tagtagtcaa aaattggtaa cagaaatcct tgatgagttg
     3721 cggaaatttg aggtaagaaa tagtagcgag tgcggttata aattctctat gttctataag
     3781 gtaggacaag gtttgattga ggacttgtcg agtgtctgtg tagatttcag cctgatttaa
     3841 caaacggtac aaatcatcca gagtgtcatt tttgaagtga ttcatcatgt caaatttatc
     3901 agtgtagtgg aggtagaagg ttcctctatt gataccagcc tttttagtaa ggtcagagac
     3961 agtaagagtt tcaaaagatt gttcagttag taaggtggtt aaggcggtct ttacataagc
     4021 tttggtttca gtatgtcttt tggtcattct caggtgctcc ttaaatcaac agttgcgtca
     4081 gatttgttct tgttaaatac ttagggccat tatataataa ggctataatt aaatcaacac
     4141 tatgttcaaa taatggaggg gcagatggct tttattgaat taaaacaggt ttcaaaatct
     4201 tatcaggtag gagagacgac tgtttttgcc aaccacgaag tgtcttttga gattaataaa
     4261 ggagaactag tggttatttt gggagcatcc ggagctggta aatcgacagt cctcaatatt
     4321 ttggggggca tggacacagt agatgcaggt caagtaatta ttgatggcaa agacattgct
     4381 cattacacgt ctaaggcctt aactcagtat cgtcggaatg ctattggttt cgtttttcaa
     4441 ttttataatt tggttcctaa tttgacagct aaagaaaatg ttgaattagc agttgaaatt
     4501 gtagcagatg ctttagatcc tgtgaccatt ttaaaggaag taggactcag tcatcgtctg
     4561 gatcattttc ctgctcagct ctcaggtggt gaacagcaac gggtttcgat agcacgtgcc
     4621 ttagctaaaa accctaaatt gcttctttgt gatgaaccta caggtgccct tgactaccaa
     4681 acaggaaagc aaattctaac cctcttacag gatatggcac gaactaaggg gaccacggta
     4741 gttattgtaa cccacaatgc tgctattgct ccgatagctg atcgtgtcat ttttatgcat
     4801 gatgcacagg ttaccaagac agtcattaat aaggaacctg caagcattga gacaattgat
     4861 tattaagagg aaggtgtcgt atgataaaga aaaccttatg gaaggatatt ttgagagcta
     4921 tcaagaatag taaagggcga ttcatttcct tatttttcct aatggcctta ggctcctttg
     4981 ctttagttgg acttaaagtc accggtccag atatggaacg aacagccagc cgttacctcg
     5041 aaagacacca ggtgatggac ctaacggtac tggcttctca tcaattttcc caagccgata
     5101 aacaagagtt agatacgtta aaaggggctc atttagaata tggtcattta cttgatgtca
     5161 gtctaactag caaccagaaa tctcttaggc tctatagcgt gccaaagaaa gtgtctaagc
     5221 cagtcttggt taaagggagt tggccaaagc gagagacaga tttggtttta tcctcctcac
     5281 ttgctaaaaa ctatcagatt ggagatgaac tagcagtcac ctcacctatg gaaggtttgc
     5341 tgacgacaac ccattttcaa gtggttggtt ttgcgaattc ttcagaagtt tggtccaagt
     5401 ccaatttagg aagttcctca accggagatg gaagtctcta cgcttatgct tttgttaatc
     5461 caaacgtttt taaaagtgcc tttaacctgc tgcgcatccg ttttagtaat cttcgtctga
     5521 caaacgcttt ttccaaagac tatcaaaaaa gggtaaccca aaaccaagct catttggata
     5581 atctacttaa agataatggc cagaagcgtt atgatgacct ccaaaatcag tatgaccttg
     5641 ccttaaaaaa tggcagagca gcacttgcaa aggaaacagt gaaactagct gcgagtgagg
     5701 agaacttaac ttttttagaa ggctctgctt tacaagaagc taagcatcag attgaacaag
     5761 gcaaacaagc attagccaag gaggaaaagc agttagagca ggtgcaggct acaaaagata
     5821 agctagaaaa acccagttac ctgacttata atcgctcgac cctaccagga ggagaaggat
     5881 atcatactta tgcaacttca acgacctcca tttcaaatgt tggaaatatt tttcctgttg
     5941 ttctttatct cgtagctgct ctagtagcct ttaccaccat gacacgttat gttgatgaag
     6001 aaagaacaag ctctggtcta ttaaaagcca ttggttattc taacaaggat atcagtttaa
     6061 agtttcttat ctatgggctt ttagccagtt ttttagggac aactttaggt attattgggg
     6121 gaacttacct cttatctgcc ttgatttcag agatcttaac aggagctttg actattggaa
     6181 agactcacct ttatagttat tggttttata atggcatagc ttacttgctg gctatgttat
     6241 ctgctgtttt accagcctac ttaattgtca aaaaggaatt attcctcaat gcagctcagt
     6301 tattgctgcc caaacctcct agtaaggggg caaaaatctg gttggaacac cttacttttg
     6361 tctggaaagc cctgtccttt actcacaagg tgaccatacg taatattttt cgctataaac
     6421 aaagaatgct gatgaccatt gtaggcgttg caggctcagt agccctttta tttgcaggct
     6481 tagggattca gtcgtcatta gccaaagtag ttgagcatca atttggtgat ttaacgactt
     6541 atgatatttt ggctgtcggt tcggccaaag cgacagcgac agagcaaact gacttagcta
     6601 gctatcttaa acaagaacct attacagggt accaaaaggt atcttatgcc agcttaaccc
     6661 ttcctgtaaa gggattacct gataagcaaa gtatttccat tttatcaagt tcagctactt
     6721 ctcttagccc ctattttaat ctgctggata gtcaggagca aaagaaggtt cccattccaa
     6781 cctctggtgt tttgatttct gagaaattag cctcctatta caaggtaaaa ccaggtgatc
     6841 agttggtatt gactgatcgg aaaggacagt cttataaagt gacgattaaa caggttattg
     6901 acatgacagt tggccatcac ctgataatgt ctgataccta ttttaagaat cattttaaag
     6961 gattggaggc tgctcctgcc tatctgatta aggtaaaaga caaagatagc aagcacataa
     7021 aggagacagc cagtgacttg ttaaccttaa aagcgattag agcagtttca caaaacgtca
     7081 atcatattaa atctgttcag ctagtagtca cctctcttaa tcaggtcatg accctccttg
     7141 tcttcttgtc tattttatta gcaatcgtta tcctttataa cttaacgact attaatattg
     7201 ctgagcgtat tcgagaatta tccactatta aagttctggg attttacgat caggaggtca
     7261 ccttatatat ttatcgagaa actatttcgc tatccctagt aggcattctt ttaggtatct
     7321 atttaggaaa aggcctgcat acttatatca tgacaatgat ttcaactggg gatattcaat
     7381 ttggtgtaaa ggttgatgct tatgtttatc tagtgccaat tctagtaatc cttagcttgt
     7441 tagcggtatt aggtatctgg gttaatcgcc atttaaaaaa ggttgatatg ttagaagctt
     7501 tgaaatccat agattgattg gtgaaaagac agatatagac agcacttgaa aagttaatca
     7561 tattatgata gtatctaaat actataattt aatagaaaga tactatgtat ggaaaatagc
     7621 attgaggcct atctagcaga tagcagagaa aaggtaacat taggtacagt tgttgattgt
     7681 tttaaaggaa aagcagtctc aagtaaggtt gtgccaggtg atgtgggatt gattaattta
     7741 tctgacatgg gcaccttagg gattcaatat caccaattaa gaactttcca gatggatcgt
     7801 aggcagctct tgcgttacct tttggaagac ggagatgtct taatcgcctc taagggaacc
     7861 ctaaaaaaag tctgtgtttt tcataagcaa aatcgagatg tggtggcatc atcaaatatc
     7921 actgtcttac gccctcaaaa actgttaagg ggctactata tcaagttctt tttggactca
     7981 cctattggtc aagcgctctt ggacgcagcg gaccatggca aagatgtgat caatctctca
     8041 acgaaagaat tgttggatat tccaattccc gttattcctt tagtgaaaca ggactatttg
     8101 attaatcact acttgagagg attaacagat tatcatcgta aactcaatcg agcagaacag
     8161 gagtgggaat atattcagaa tgaaattcaa aaaggattag gttaggagtc ttgtcatggg
     8221 ttcagttgaa cgtacattaa aaatgacctt ggctaccatt gttgccattt taattgctta
     8281 ccaacttcat ctggattatg caatgtcagc ggggatcatt gccctgttaa gtgttttaga
     8341 cacccgtaag tccagtttgg tggtcgctag gaatcgcttg ctatcttttt tcctggcttt
     8401 cgggattgcg atgatgtgct ttagcttgtt tggttttacg acagtaggtt ttatgtgtta
     8461 tttgttaatt attattcctt tgctctatca ctttcagata gaggcaggac tggttcctat
     8521 aacagtactt gtgacacatt tgattgctaa aaaaagtatt gcattaccta ttttatcgaa
     8581 tgagtttatg ctcttttttg tagggactag tgtcgcttta ctatttaatg cttatatggg
     8641 tccacaagat cagcaaattc ggtactatca ccaaaaagtt gaatcggatc tgaaaggtat
     8701 cttataccgt tttgaaagtt ttctactgga aggaaaaggt caaaatgaag ggctcttgat
     8761 caaaaacctt gataaaatat tagatgaagc tctaaaacta gtttatcgag aaaggcataa
     8821 tcaactattt cagcagacca attatcaagt tcattatttt gagatgcgac gacaacaaaa
     8881 tagactattg ggacaaatgg ctatcaacgt aaacacgtta atgagacaaa gtaaggaaag
     8941 tattcttttg tcacatcttt ttcacgaaac tgcttgtcag ctaagcgaac aaaatccggc
     9001 tttaaccttg attgatgaca tcgaacaatt gcttgaaacc tttcgtcatg gtgatcttcc
     9061 tcaaactcgg gaggaatttg agcgacgtgc ggttttattt cagctcttac aagacttgga
     9121 acgttttatc ttattaaagg tagagtttta tcaggattat caaaatgact aaatctctat
     9181 tgcacacttc ttatctatga cagcttggga cttaacttcc tgagcttttt tgctataata
     9241 aaaacaagaa tctcagaatg gagacaagat gaacgaaata aaatgccctc attgtcatac
     9301 cctatttact atcaatgagt cagagtatag tcaattattg gaacaagtca gagggcaagc
     9361 ctttgatgaa gaattgcaaa aacggctaat caatgagata gccttgctgg aagaaaaagc
     9421 gaagcatcaa ttgcatgagg ttgtcgcaaa aaaagaaaca gctattacca gcttgacaaa
     9481 ccaattagag caaattgaaa aagaacaagc ctacctcaga caagaagaac tggctaaaaa
     9541 agaccagctt attgcttctt tagaagcgaa acttgataaa ttagcctcac aaaatgcttt
     9601 ggaactggcc aatcaattag ctgagaagga caaagaagtg gttagtttga ccaatcagct
     9661 ggacaaatta gcacttgaaa aagatgccac gttccagtca aaacttgcta caatcgaaaa
     9721 agaacgcgat ggaatcaaaa accaattggc gcttcaagct aaagaaagtg aattgtcgtt
     9781 ggcttcggtg cgcagcgact acgaagcaca gttaaaagcg gctaacgagc aagttgaatt
     9841 ttataaaaat ttcaaagcgc agcaatctac caaagcaatc ggagagagtc tagaactcta
     9901 tgctgaaaca gagtttaaca aagtaagaag ctatgctttt ccgaatgcta gctttgtcaa
     9961 agataaccaa ttgtctagtc gtgggtcaaa aggggactat atttaccgag aggttgatgc
    10021 caatggtgta gaaatcctct ctatcatgtt tgaaatgaag aacgaggcag acaccaccaa
    10081 aacaaaacat aaaaatagtg attttttcaa ggagttagat aaagaccgtc gcgaaaaaga
    10141 ttgtgagtat gctgttttgg taagcatgtt agaagctgat aatgattact acaatacagg
    10201 gattgttgat gtcagtcatg agtatcaaaa gatgtatgtg gttcgtcctc aactctttat
    10261 tcagttgata gggattctgc gaaatgcagc cctgaatagt ttacattata agcaagagtt
    10321 ggccttggtt aaggaacaaa atattgacat cactcacttc gaagaggatt tggaccaatt
    10381 taagaatgcc tttgctaaga actatcagtc tgccagcaac aactttaaaa aggctattga
    10441 cgagattgat aaatcgatta aacgcatgga ggaagtgaaa cgctttttaa ccactagtga
    10501 aaatcagtta cgcttagcta ataataaatt agaggatgtt tcagttaaaa aactgacccg
    10561 ccaaaaccca accatgcgag agaaatttga agccctcaag gaccagtaag cctgaaagtg
    10621 agtctttcct tgctaaaatt ggttaagcgt ttattttggc aaaactgatg ataaagcggt
    10681 agaatagaat cagtaataat tactaattag agagaaaaat agacatgata aatggaatca
    10741 ttaatttaaa aaaagaagcc ggcatgacct ctcatgacgc agtttttaaa ttacggaagt
    10801 tattacaaga aaaaaagatt ggacatgggg ggaccttaga tcctgatgtg gtcggtgttc
    10861 taccaattgc agttggtaag gctactcgtg tgatcgaata catgacagaa gctggtaaag
    10921 tttatgaagg acaagtgact ctcggttact caacaacgac tgaggatgct agtggagagg
    10981 tagttgcgag gtcctctttg ccagctgtgc taacagaaga actcgttgat caaacgatga
    11041 cgactttcct tgggaaaatc acacagactc cacctatgta ttcagccgta aaggttaatg
    11101 gccgtaaatt atacgagtat gcgcgtgctg gtgaaagtgt ggaacgtcct agaagagaag
    11161 tgactatttc tctgtttgaa cgcaccagcc ctttgaattt tactgaagac ggtctttgcc
    11221 gattctcttt taaagttgct tgtagcaaag ggacttatgt gcggacctta gcagttgatt
    11281 taggtcgagc tctaggggta gagagtcata tgtccttctt gcagcgatca gcttcagcag
    11341 gattaactct cgagactgcc tatactttgg gagaaattgc tgatatggtt tctaaacaag
    11401 agatgtcttt tttgttaccg atcgagtatg gcgttgctga tcttcctaaa atggttattg
    11461 atgatactga attgactgaa atttcatttg gtcgccgtct tagtctccct agtcaagagc
    11521 cactgttagc tgcttttcat ggcgaaaaag taattgctat tttagaaaaa agagatcaag
    11581 aatacaaacc caaaaaag
//
LOCUS       DAUCWU010000051        11493 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUCWU010000051 DAUCWU010000000
VERSION     DAUCWU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.051"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            83..2323
                     /locus_tag="VGY24_001214"
     CDS             83..2323
                     /locus_tag="VGY24_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ4284194.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRSSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDSAQINPDESFKTEAQSNGINDQQLGLMR
                     KALKELIDPNLGSKYSNKTPSGYRLNVFESHDKTFQNLLSAEYVPDTPPKPGEEPPAK
                     TEKTSVIIRKYAEGDYSKLLEGATLRLTGEDIPDFQEKVFQSNGTGEKIELSNGTYTL
                     TETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVGSPYTIEAYNDFDEFGL
                     LSTQNYAKFYYGKNYDGSSQIVYCFNANLKSPPDSEDHGATINPDFTTGDIRYSHIAG
                     SDLIKYANTARDEDPQLFLKHVKKVIENGYHKKGQAIPYNGLTEAQFRAATQLAIYYF
                     TDSVDLTKDRLKDFHGFGDMNDQTLGVAKKIVEYALSDEDSKLTNLDFFVPNNSKYQS
                     LIGTEYHPDDLVDVIRMEDKKQEVIPVTHSLTVKKTVVGELGDKTKGFQFELELKDKT
                     GQPIVDALKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETEAKDYIVTVD
                     NKVSQEAQSASENVTADKEVTFENRKDLVPPTGLTTDGAIYLWLLFLVPFGLLVWLFG
                     RKGTKK"
     gene            2320..2841
                     /gene="lepB"
                     /locus_tag="VGY24_001215"
     CDS             2320..2841
                     /gene="lepB"
                     /locus_tag="VGY24_001215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4284195.1"
                     /translation="MTNYLNRLNENPLLKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            2863..3918
                     /locus_tag="VGY24_001216"
     CDS             2863..3918
                     /locus_tag="VGY24_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ4284196.1"
                     /translation="MKKNKLLLASTILATALGMNNLASAEVNYVKSGVIDGAKLEIHK
                     TIKNYDDDKVLMPNVIFKFNIKPDDSVVAGKKEEKSGLDIKPGIVDGLETSKEVSYTN
                     SDKPNSKDKYTYFDFSQVKFTNVGIYRYIVEEVQDTVSGIHYDSQKWYIDVYVVEGDN
                     GFVPKYIVSSKALETKEPVLFSNSFDTTSLVVKKEVTGNTGDKTKNFKFQLLLKENAY
                     FSAGQKVSVTITSEKASEVTTATIGQPLIFELKHNEQLKLDKLPVGITYQIDETSKNS
                     DSYTTTATIQEGEQSQNSYVLNSDKETDKSPDIITVTNKRDTQVPTGVVGTLAPFAVL
                     SIVAIGGVIYITKRKKA"
     gene            3934..4659
                     /gene="srtB"
                     /locus_tag="VGY24_001217"
     CDS             3934..4659
                     /gene="srtB"
                     /locus_tag="VGY24_001217"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ4284197.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKYHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            4676..5263
                     /locus_tag="VGY24_001218"
     CDS             4676..5263
                     /locus_tag="VGY24_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ4284198.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
     gene            complement(5422..6627)
                     /locus_tag="VGY24_001219"
     CDS             complement(5422..6627)
                     /locus_tag="VGY24_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ4284199.1"
                     /translation="MVIFDLKHVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            7012..10506
                     /locus_tag="VGY24_001220"
     CDS             7012..10506
                     /locus_tag="VGY24_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ4284200.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGAFEIKKVDQNNKPLSGATFSLTPKDGKGTSVQTFTSNDKGIVDAQNLQPGTYTLK
                     EDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     FSKYGGAEVTSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDRRLNPKGI
                     SQDIPKIIYDSANSPLAIGKYDAESHQLIYTFTDYIAGLDKVQLSAELSLFLENKEVL
                     ENTNISDFKSTIGGQEIKYGGTVNVIYGNEKNQKDNYVANGLSNVGGSIESYNTQTGD
                     FTWYVYVNPNRTNIPYATMNLWGFGRLVKSNNSTSQSEDSATSSAELGEIQVYEVPEG
                     EKLPSSYGVDVTKLTLREEFKPQVVQGSFGTTKRQRIDFGNNIQNKAFIIKVTGKTDQ
                     SGKPLVVQSNLASFSTKEDYAGLKLGGGNVYFQNEIALSPSKGSGSGKSEFTKPSITV
                     ANLKRVAQLRFKKMSTDNVPLPEATFELRSSTSDSQKVEAKSNEQGEVHLKDLTSGTY
                     DLYEKQAPKGYQQVTEKLATVTVDTSKSAEEMVTWGSPHSSVKVEVNKEVTIVNHKET
                     LTFSGKKIWENDRPDQRPAKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYDAK
                     NQEYKYSVEEVNVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKI
                     IDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKN
                     LKKYNGTGNDIIYSVKEVAVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLES
                     GASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATM
                     ELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETVAPDGYEVATAITFTVNEQGQVTV
                     NGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKPSDVIIGG
                     QGEVVDTTEDTQRGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDSGC
                     KTEVEDTKLVQSFHFDNKEPESNSEIPKKDKPKSNTSLPATGEKQHNKFFWMVTSCSL
                     ISSVFVISLKSKKRLSSC"
     gene            complement(10771..11436)
                     /locus_tag="VGY24_001221"
     CDS             complement(10771..11436)
                     /locus_tag="VGY24_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284201.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKTLTADEKSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTDITYEHHY
                     WDGTVHSEKHNNTQWHYDWENQQTNFNQEFDKFPGYTGWSRSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEGNKDKWNKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         3946 a         1850 c         2283 g         3414 t
ORIGIN      
        1 aaatgatata gtagctatga aatagcattg tgactgtccg gtaaagggat gggactgaag
       61 attttattca gaaggagagg gaatgaataa caaaaaattg caaaagaagc aagatgctcc
      121 tcgggtatca aacagaaagc caaaacaatt aactgtcact ttagtgggag tatttttaat
      181 gctcctagta ttgataggat ttgaaggaaa agttcgagca gctcacgaat tggttgaggt
      241 accagtacct atttttcaca atccagatcc acagtcagac tatcagtggt atggctatga
      301 agcctataca ggggggtatc cgaagtatga tttattcaag acatactatc atgatctgag
      361 agtgaatttg catgggagta aaagttatca agtatattgc tttaacgttc acaaacacta
      421 tcctcgttcc tcccagagtt ttgatcggaa gtggtataaa aaattagatg gaactgctga
      481 gaattttgat tctcttgcta tggaaccaag agttcgtaaa gaggagttaa ccaaaaaact
      541 ccgagctgtt atgtataatg cctatcctaa tgatgctaat ggcattatga aagatttaga
      601 gcctttaaat gctattaaag tcactcaaga ggctgtatgg tactattctg attcagctca
      661 aattaatccg gatgaaagtt ttaaaacaga agctcaaagt aatggtatta atgaccagca
      721 gttaggctta atgcgaaaag ctttaaaaga actaattgat ccaaacttag ggtcaaaata
      781 ttcgaataaa actccatcag gttatcggtt aaatgtattt gaatctcatg ataagacttt
      841 ccaaaatctt ttgagtgctg agtatgttcc ggatactccc ccaaaaccag gagaagagcc
      901 tccggctaaa actgaaaaaa catcagtcat tatcagaaaa tatgcggaag gtgactactc
      961 taaacttcta gagggagcaa ctttgcgttt aacaggggaa gatatcccag attttcaaga
     1021 aaaagtcttc caaagtaatg gaacaggaga aaagattgaa ttatcaaatg ggacttatac
     1081 cttaacagaa acatcatctc cagatggata taaaattgcg gagccgatta agtttagagt
     1141 agtgaataaa aaagtattta tcgtccaaaa agatggttct caagtggaaa atccaaacaa
     1201 agaagtaggt tctccatata ctatagaggc atacaatgat tttgatgaat ttggcttact
     1261 gtcaacacaa aattatgcga aattttatta tggaaaaaac tatgatggca gttcacaaat
     1321 tgtttattgc ttcaatgcca acttgaaatc cccacctgac tcggaagatc atggtgctac
     1381 aataaatcct gactttacga ctggtgatat taggtatagt catattgctg gttcagattt
     1441 gataaaatac gctaatacag ctagggatga agatcctcaa ttatttttaa aacacgtaaa
     1501 aaaagtaatt gaaaatgggt atcataaaaa aggtcaagct attccatata acggtctgac
     1561 tgaggcacag tttcgcgcgg ctactcaact ggcaatttat tattttacag atagtgttga
     1621 cttaactaag gatagattga aagacttcca tggatttgga gatatgaatg atcaaacttt
     1681 gggtgtagct aaaaaaattg tagaatacgc tttgagtgat gaagattcaa aactaacaaa
     1741 tcttgatttc ttcgtaccta ataatagcaa ataccaatct cttattggga cagaatacca
     1801 tccagatgat ttggttgacg tgattcgtat ggaagataaa aagcaagaag ttattccagt
     1861 aactcatagt ttgacggtga aaaaaacagt agtcggtgag ttgggagata agactaaagg
     1921 cttccaattt gaacttgagt tgaaagataa aactggacag cctattgtcg acgctctaaa
     1981 aactaataat caagatttag tagctaaaga tgggaaatat tcatttaatc taaagcatgg
     2041 tgacaccata agaatagaag gattaccgac gggatattct tatactctga aagagactga
     2101 agctaaggat tatatagtaa ccgttgataa caaagttagt caagaagctc aatcagcaag
     2161 tgagaatgtc acagcagaca aagaagtcac ttttgaaaac cgaaaagatc ttgtcccacc
     2221 aactggtttg acaacagatg gggctatcta tctttggttg ttatttcttg ttccatttgg
     2281 gttattggtt tggctatttg gtcgtaaagg gactaaaaaa tgactaatta cctaaatcgc
     2341 ttaaatgaga atccactatt gaaagctttc atacggttag tacttaagat ttctattatt
     2401 ggatttctag gttacattct atttcagtat gtttttggcg tcatgattgt taacacaaat
     2461 cagatgagtc ctgctgtaag tgctggtgat ggagtcttat attatcgttt gactgatcgc
     2521 tatcatatta atgatgtggt ggtgtatgag gttgataaca ctttgaaagt tggtcgaatt
     2581 gccgctcaag ctggcgatga ggttaatttt acgcaagaag gaggactgtt gattaatggc
     2641 catccaccag aaaaagaggt cccttacctg acgtatcctc actcaagtgg tccaaacttt
     2701 ccctataaag ttcctacggg tacgtatttc atattgaatg attatcgtga agaacgtttg
     2761 gacagtcgtt attatggggc gttacccatc aatcaaatca aagggaaaat ctcaactcta
     2821 ttaagagtga gaggaattta aaaaaatttg gagagagaga aaatgaaaaa aaacaaatta
     2881 ttacttgcaa gtacaatttt ggctactgct ttgggaatga ataatttagc atcagctgaa
     2941 gtaaattatg taaaatcagg agttattgat ggtgcaaagt tagaaatcca taaaacaatt
     3001 aagaattatg atgatgataa agttttaatg ccaaatgtga ttttcaagtt taatattaag
     3061 ccagatgatt cagttgttgc tggtaaaaaa gaagaaaaat cgggattaga tataaaacca
     3121 ggtatagttg atgggttgga aactagtaag gaagtatctt atacaaatag cgacaaacca
     3181 aatagtaaag ataaatatac ttactttgat ttttctcaag tgaaatttac taatgttggt
     3241 atctatcgct atattgttga agaagtacag gatacagtat ccggtattca ttatgatagt
     3301 caaaaatggt atatagacgt ttatgttgtt gaaggagata atggattcgt tccgaaatat
     3361 attgtatcaa gtaaagcatt ggaaactaaa gaaccagttt tatttagtaa ttcatttgat
     3421 acaacttctt tagttgttaa aaaagaggtt acaggaaata ctggagataa aacgaaaaac
     3481 tttaaattcc aattattatt aaaagagaat gcttatttct cagcaggtca aaaagtttca
     3541 gttacaataa cttctgagaa agcttcagaa gtcacgactg ctaccatagg tcaaccactt
     3601 atctttgagt taaaacacaa tgaacaactt aaattggata aattgcctgt tggtattacg
     3661 tatcaaattg atgaaactag taaaaatagt gacagctaca caacgactgc tacaattcaa
     3721 gaaggtgaac aatcacaaaa ttcttatgtt ttaaactcag ataaggaaac tgataaaagt
     3781 ccagatatta ttacagtcac aaataagcgt gacactcaag ttccaactgg tgttgtaggc
     3841 acccttgctc catttgcagt tcttagcatt gtggctattg gtggagttat ctatattaca
     3901 aaacgtaaaa aagcttaagt gattagtcaa agaatgatga tgacaattgt acaggttatc
     3961 aataaagcca ttgatactct cattcttatc ttttgtttag tcgtactatt tttagctggt
     4021 tttggtttgt gggattctta tcatctctat caacaagcag acgcttctaa tttcaaaaaa
     4081 tttaaaacag ctcaacaaca gcctaaattt gaagacttgt tagctttgaa tgaggatgtc
     4141 attggttggt taaatatccc agggactcat attgattatc ctctagttca gggaaaaacg
     4201 aatttagagt atattaataa agcagttgat ggcagtgttg ccatgtctgg tagtttattt
     4261 ttagatacac ggaatcataa tgattttacg gacgattact ctctgattta tggccatcat
     4321 atggcaggta atgccatgtt tggcgaaatt ccaaaatttt taaaaaagga ttttttcaac
     4381 aaacataata aagctatcat tgaaacaaaa gagagaaaaa aactaaccgt cactattttt
     4441 gcttgtctca agacagatgc ctttgaccag ttagttttta atcctaatgc tattaccaat
     4501 caagaccaac aaaggcagct tgttgattat atcagtaaaa gatcaaaaca atttaaacct
     4561 gttaaattga agtatcatac aaagttcgtt gctttttcaa cgtgtgaaaa tttttctact
     4621 gacaatcgtg ttatcgttgt cggtactatt caagaataac gaaaggagga gacttttgag
     4681 aaaatattgg aaaatgttat tttctgtcgt aatgatatta accatgctgg cctttaatca
     4741 gactgtttta gcaaaagaca gcactgttca aactagcatt agtgtcgaaa atgtcttaga
     4801 gagagcaggc gatagtaccc cattttcgat tgcattagaa tcaattgatg cgatgaaaac
     4861 aatagaagaa ataacaattg ctggttctgg aaaagcgagc ttttcccctc tgaccttcac
     4921 aacagttggg caatatactt atcgtgttta tcagaagcct tcacaaaata aagattatca
     4981 agcagatact actgtatttg acgttcttgt ctatgtgacc tatgatgaag atgggactct
     5041 agtcgcaaaa gttatttctc gaagggctgg agacgaagaa aaatcagcga ttacttttaa
     5101 gcccaaacgg ttagtaaaac caataccgcc tagacaacct gacatcccta aaaccccatt
     5161 accattagct ggtgaagtaa aaagtttatt gggtatctta agtatcgtat tactggggtt
     5221 actagttctt ctttatgtta aaaaactgaa gagtaggcta taaaacccat ttttaagagt
     5281 cacacatttt tagttgctct atttttataa tgaaacttat tgacatctcc acagtcttgc
     5341 ttgactttgg agatgttttc tatgctgata ggtttttgta ccatataaga taatgattga
     5401 tgaaaagagt gtcttgaatt cttaaatatt atctcggtat ttagcaagat agtcttttga
     5461 agaaataccc gtgtattttt taaagatttt atgaaaatgg gtagtgtcat aaaaatataa
     5521 gcttttagcc acttctccaa caggaattcc tcgttttaag agtagttgag cttctttgat
     5581 ttttgtactt agaatataat gttgtaagga aacatttgag tattttttaa agactgaacg
     5641 taagtgagat tcggaaacat ataggcgctt agcgatatca gataccttta atttggaata
     5701 caggtgacta tcgacataat gaagcacact attgtacatt tgacgtttat cagaaatact
     5761 tttattggtt aatgattcgg aaaaatggat gatggctgca cagcgaattc tcaaaacttt
     5821 gagcaagtca ctcgcttctt catttaaggc catgtagtat ttgacaaggc tataatgctt
     5881 tgctacgtca gtattttcta caaaactgaa ttccagtaac ctagttaaat agataacagt
     5941 gaggttcttc tccgccctta gagcagaaat agaagatgag gtgatacttg ataaagggat
     6001 gcgattgatt tcttgcttga gcaattgtgg attgcctagc tttaccagat ccataatatg
     6061 atttaggtgc tctaaaacag gcggtagttg gctagatgct ttgctaatat gcttaaggcg
     6121 atgaatgata tcaggatcag cagtcatctg ttttgaatgc tgcaacaatt gatgaattgt
     6181 ttgttggcaa gtcgtctcaa attgtgtgtc aaaaaaagcg tcaatagcta tcaaaaggtt
     6241 ccgcacatta ttaattggaa aatgtgggac aagtgccata taggataaga gctcttcttt
     6301 gctacgatga gaagaatgtt ttaaaaaatt attagctaat tgttcctgat agtctttatt
     6361 aagtgaataa gggtagaaag gtccaatggc aataatgtag tgacaaagag gaaaaatcat
     6421 aaaggactct tcaaataaac cctcataaaa aataacatct tgtgcagctt gaggaatagc
     6481 tagatgcttt aaaaattgat agtaacataa taaatagtcg tcattaccat atacttgaat
     6541 aagtgcctta tcttgtgaca tcactgaaat aggtaattga gacaagctgt gtaatgtttg
     6601 cacatgtttt aaatcgaata taaccatttg atagattgcc ccccatcctt agctgtatca
     6661 ttttccttag acttgtaaaa gctttagtaa atgttaatga tgaaaattcc tatttggtag
     6721 gcacactaaa aggtcttcct aatagtaaac tctctctcca tcatctcctg ccttaattat
     6781 actatgaata gctaagcaga taaagataaa ggacttgtac gttaagcgct tgaaaaagaa
     6841 agagttacag ataatgacat aaaaaacgct aaaaaaccat caaaataaat actctgacca
     6901 taagatgtag acttgacaac tgaaaatggt aaaataacta tttgacagtt gtcctgtagt
     6961 ctttagtttt ggacataggc tgtcgcttat gaatgtggag agagaaaata aatgacacaa
     7021 aaaaatagct ataagttaag cttcctgtta tccttaacag gatttatttt aggtttatta
     7081 ttggttttta taggattgtc cggagtatca gtaggacatg cggaaacaag aaatggggca
     7141 aacaaacaag gagcttttga aatcaagaaa gtcgaccaaa acaataagcc cttatcggga
     7201 gcaacttttt cactgacacc aaaggatggg aagggaacat ctgttcaaac gttcacttca
     7261 aatgataaag gtattgtaga cgctcaaaat ctccaaccag ggacttatac cttaaaagaa
     7321 gataaagcac cagatggtta tgataaaacc agtcggactt ggacagtgac tgtttatgag
     7381 aacggctata ccaagttggt tgaaaatccc tataatgggg aaatcatcag taaagcaggg
     7441 tcaaaagatg ttagtagttc tttacagttg gaaaaatcaa caatgtctgt attctctaaa
     7501 tacggagggg cagaagtaac tagtggcgca gcggatttct accgcaacca tgccgcctat
     7561 tttaaaatgt cttttgagtt gaaacaaaag gataaatctg aaacaatcaa cccaggtgat
     7621 acctttgtgt tacagctgga tagacgtctc aatcctaaag gtatcagtca agatatccct
     7681 aaaatcattt atgacagtgc aaatagtccg cttgcgattg gaaaatacga tgccgagagc
     7741 catcaactta tctatacttt cacagattat attgcaggtt tagataaagt ccagttgtct
     7801 gcagaattga gcttattctt agagaacaag gaagtgttgg aaaacaccaa catttcagat
     7861 tttaagagta ccattggtgg tcaggaaatc aaatatggtg ggacagttaa tgttatttac
     7921 ggaaatgaaa aaaatcaaaa agacaactat gttgctaacg gattgagcaa tgtgggtgga
     7981 agtattgaaa gctacaatac acaaacaggc gactttactt ggtatgttta tgtcaaccca
     8041 aatcgtacca atattcctta tgcgaccatg aatttatggg gatttggaag gttggttaaa
     8101 tctaataact caacaagtca gtcagaggat tccgctacaa gtagtgctga gcttggagag
     8161 attcaggtct atgaagtacc tgaaggagaa aaattaccat caagttatgg ggttgatgtt
     8221 acaaaactta ctttaagaga agaattcaaa cctcaagttg ttcagggtag ttttggaact
     8281 accaaacgtc agcgaattga ctttggaaat aatatccaaa ataaagcatt tatcatcaaa
     8341 gtaacaggga aaacagacca atctggtaag ccactagtcg ttcaatccaa tttagctagt
     8401 ttctcaacta aagaggatta cgcgggatta aaattaggtg gaggaaatgt ctacttccaa
     8461 aacgaaattg ccttgtctcc ttctaagggt agtggttctg ggaaaagtga atttactaag
     8521 ccctctatta cagtagcaaa tctaaaacga gtggctcagc ttcgttttaa gaaaatgtca
     8581 actgacaatg tgcctttgcc agaagcgact tttgagctgc gctcatcaac tagtgacagt
     8641 caaaaagtag aagcaaaatc aaatgaacaa ggagaggttc accttaagga cctgacctcg
     8701 ggcacctatg acttgtatga aaaacaagca cctaaaggct atcagcaggt gacagagaaa
     8761 ttagcgaccg ttactgttga tactagcaaa tctgctgagg aaatggtcac ttggggaagc
     8821 ccacattcgt ctgtaaaagt ggaagttaac aaagaagtca cgattgtcaa ccataaagaa
     8881 acccttacgt tttcagggaa gaaaatttgg gagaatgaca gaccagatca acgcccagca
     8941 aagattcaag tgcaactgtt gcaaaatggt caaaaaatgt ccgataaagt tcaagaagta
     9001 acgaaggata acgattggtc ttatcacttc aaagacttgc ctaagtacga tgccaagaat
     9061 caggagtata agtactcagt tgaagaagta aatgttccag acggctacaa ggtgtcgtat
     9121 ttaggaaatg atatatttaa caccagagaa acagaatttg tgtttgaaca gaataacttt
     9181 aaccttgaat ttggaaatgc tgaaataaaa ggtcaatctg ggtcaaaaat cattgatgaa
     9241 gacacgctaa cgtctttcaa aggtaagaaa atttggaaaa atgatacggc agaaaatcgt
     9301 ccccaagcca ttcaagtgca gctttatgct gatggagtgg ctgtggaagg tcaaaccaaa
     9361 tttatttctg gctcaggtaa tgagtggtca tttgagttta aaaacttgaa gaagtataat
     9421 ggaacaggta atgacatcat ttactctgtt aaagaagtag ctgttccaac aggttatgat
     9481 gtgacttact cagctaatga tattattaat accaaacgtg aggttattac acaacaagga
     9541 ccgaaactag agattgaaga aacgcttccg ctagaatcag gtgcttcagg tggtaccact
     9601 actgtcgaag actcacgccc agttgatacc ttgtcaggtt tatcaagtga gcaaggtcag
     9661 tccggtgata tgacaattga agaagatagt gctacccata ttaaattctc aaaacgtgat
     9721 attgacggca aagagttagc tggtgcaact atggagttgc gtgattcatc tggtaaaact
     9781 attagtacat ggatttcaga tggacaagtg aaagatttct acctgatgcc aggaaaatat
     9841 acatttgtcg aaaccgtagc accagacggt tatgaggtag caactgctat tacctttaca
     9901 gttaatgagc aaggtcaggt tactgtaaat ggcaaagcga ctaaaggtga cgctcatatt
     9961 gtcatggttg atgcttacaa gccaactaag ggttcaggtc aggttattga tattgaagaa
    10021 aagcttccag acgagcaggg ccattctggc tcaactactg aaatagaaga cagcaagcct
    10081 tcagacgtta tcattggcgg tcaaggtgaa gttgttgaca caacagaaga cacacaacgt
    10141 ggtatgacag gccattctgg ctcaactact gaaatagaag atagcaagtc ttcagacgtt
    10201 atcattggtg gtcaggggca ggttgtcgag acaacagagg atacccaaac tggcatgcac
    10261 ggggattctg gttgtaaaac ggaagtcgaa gatactaaac tagtacaatc cttccacttt
    10321 gataacaagg aaccagaaag taactctgag attcctaaaa aagataagcc aaagagtaat
    10381 actagtttac cagcaactgg tgagaagcaa cataacaagt tcttttggat ggttacttct
    10441 tgctcactta ttagtagtgt ttttgtaata tcactaaaat ccaaaaaacg cctatcatca
    10501 tgttaagata agctgttggt gacaatagcg aaaaagggtc cacttattag tgggcctctt
    10561 tttctggaat cgatcgtctc ggttcactag ataagtcatt ttaagtagtt caaaaaggca
    10621 gagccttaaa aggttttctg cctttttaat cttgttgggt acttacgaaa gtatttcggc
    10681 cacttcaagc ataataaaaa gagtctgagc aatcatgttg tctcagactc tgactgtatt
    10741 atgcaaaaga tgatggactg gcagcttcat ttactcaccg aactcccctt tataacttgg
    10801 aaatgatgct gctaatgggt tgttttgttt aatacctgct ataaacggta atttattcca
    10861 tttatctttg ttaccttctt caagctcagt tttaaacttc caaaccattt tatgagaaaa
    10921 ataagtgtgt gattgacggc cataggtgtc tactaaaacg tgagaaaatt ttccgtcgtt
    10981 ttttagtttg taaaatttat ctagagaacg actccagcca gtataaccag ggaatttatc
    11041 aaattcttga ttaaaatttg tttgttggtt ttcccaatca taatgccatt gtgtattatt
    11101 gtgtttttca gaatgaactg taccgtccca ataatgatgc tcataagtta tatccgtagg
    11161 agcttttaca cccttaggaa gaaaaactga gaaatcaggg taaacccatt gttctttagc
    11221 tgaattaaag gttacagtcc attcgatact tttttcatct gcagttagtg ttttagtata
    11281 gtcgacatag ttatcaacat agtaatttcc ctgccagtaa aggtgcactt tacctttctc
    11341 atcaatgtta tcatgggcat gtactgattt atacgaacta aacagcatcg aagctgctag
    11401 tagtgaagtg aagagatatt tattacatcg tttcatatca tgaaaaacct ttcttttaat
    11461 actaatatat tatatcaaaa aaactaaaaa gta
//
LOCUS       DAUCWU010000052        10883 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUCWU010000052 DAUCWU010000000
VERSION     DAUCWU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.052"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..981)
                     /locus_tag="VGY24_001222"
     CDS             complement(<1..981)
                     /locus_tag="VGY24_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ4284202.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKHLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTYYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEI"
     gene            complement(978..1985)
                     /locus_tag="VGY24_001223"
     CDS             complement(978..1985)
                     /locus_tag="VGY24_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ4284203.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNEINIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEVSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(2010..3215)
                     /locus_tag="VGY24_001224"
     CDS             complement(2010..3215)
                     /locus_tag="VGY24_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284204.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            complement(3215..4018)
                     /locus_tag="VGY24_001225"
     CDS             complement(3215..4018)
                     /locus_tag="VGY24_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284205.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            complement(4021..4953)
                     /locus_tag="VGY24_001226"
     CDS             complement(4021..4953)
                     /locus_tag="VGY24_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4284206.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(4943..6097)
                     /locus_tag="VGY24_001227"
     CDS             complement(4943..6097)
                     /locus_tag="VGY24_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ4284207.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDISEKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDHLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            complement(6216..7070)
                     /gene="rfbD"
                     /locus_tag="VGY24_001228"
     CDS             complement(6216..7070)
                     /gene="rfbD"
                     /locus_tag="VGY24_001228"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ4284208.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDHPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(7208..7546)
                     /locus_tag="VGY24_001229"
     CDS             complement(7208..7546)
                     /locus_tag="VGY24_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ4284209.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(7782..8891)
                     /gene="rpoD"
                     /locus_tag="VGY24_001230"
     CDS             complement(7782..8891)
                     /gene="rpoD"
                     /locus_tag="VGY24_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ4284210.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(8900..10705)
                     /gene="dnaG"
                     /locus_tag="VGY24_001231"
     CDS             complement(8900..10705)
                     /gene="dnaG"
                     /locus_tag="VGY24_001231"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ4284211.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELL
                     YQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERD
                     LHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
BASE COUNT         3324 a         2150 c         1832 g         3577 t
ORIGIN      
        1 gatctctttt cttttgatat cactatctgt tgtaataaaa agatcataat ggaagttcca
       61 gtcttcgaaa gctgttaaaa attcatctag caaatcaacg taaaaaacat gtaaatgaac
      121 agctaccttt tgacaagttt gttttgctaa ccgttggcag tttaggtatt tttgagataa
      181 aaggtactta gcatctggca aagagatttg cgacatatgt gaaacaataa gatctatagg
      241 ataataggtt gtttctcgta tcagattcag taagtaagga gcgatatgct ggttagcatc
      301 aatagctttt acttttataa aaggcacatg gtgttttaga attgctgttg gattataata
      361 tgaaaaatcc ggatgtggca tgaatgacga ctcagctttt ctagtatcaa agaccgtctg
      421 ataactaaag ccttcattta agagaatgga tgttaattgc gtttcataat gctgaatcac
      481 ttcttgaacg ttttcatatt ctattatcga ttgccaaaat tgttgaaaaa ctttgttttg
      541 aatgacttga tttttaaacg tcataaaata actttggaca tgttctttaa aagcttttgt
      601 acctctatta ttagtaattc cccaaaagtc aactgcctct ttttcttcaa aattttcaaa
      661 ataaggaccc atctcccaaa taggaccaaa acaagtatcg ttcataatcg tcaatgagtc
      721 aaactcttcc aacttgtcaa atcccatagt aatcaagcca tcgtgccatg cagaaaaatc
      781 aaaaccttta tttttgcgtt gcaaaaactc atcaataaga cagtggtttt tgagatgttt
      841 taaatcttca tgactcactt tactatttga gataaaaaca atcttagaaa acagtgatct
      901 catttgttcc aattgatagt aaacatgtgc acttatttta ttgtatttgt tgaagtgaac
      961 atataataaa atacgattca tttttttctc ctaataaaag caatgagctt agacgtgtat
     1021 ttccattgac gtgaattaat aatggcatgg tattgttgtc ttaattcaga tagttgattt
     1081 ttttgttctt ctatgacatt ttgcaactcc acttcacgtg tttcttgctg ctttgtttta
     1141 tctacgatag tttgttgtaa ttcttgaata aagcaactca actcatgaat tcgtatttct
     1201 tgcttagcat cttgttgtaa taatagttca gacacttctt ttgtatctac ctgattatac
     1261 ttttttaagt aatttaactg agtatcaagt agtttttgaa tacgatgaat catttttggt
     1321 gtatcagtag gtcttgaatc agaatcggtt gtcatgcgat aaaccgtcgt tgagtcttta
     1381 atataggtca gttgagtcag ttgaaataac tctagttgaa tgtcaaaagt atcgtctggt
     1441 gtatctacat tgattttttg gtttacttgt ctcattaaat cagcatcaac taaccatgtc
     1501 gatgccatcg tcatcccttt taaggctaaa actttttcgt aagtatctgt caaaggtgta
     1561 tgaccagatg aaaaagcatt agcatataat aagtcgcctt gactatttac aaagtcaaaa
     1621 tcagtattac accatcgtga ccgtttggaa gcttctaaga catcaacttg tttttggagt
     1681 ttaaaactat ccgtccagta atcatcacca tcacatctag caatatattt gccttttgca
     1741 taaaggcatg cttttatcca agtctttgtt attccaatat taatttcgtt aaagagtaat
     1801 ttaatttttc ctgaagactt tttttgataa ctctttaaaa tctctcgtga gtcatctgta
     1861 gaagcatcat caataacaat aatttcaact tcaaaatctg tcacttggct taaaaaactg
     1921 tcaagtgcat cagatatcca aggagctttg ttataattgg tgcaaataat tgaaacttta
     1981 tacataaatt cccttctagc tattactcat taatgaaaaa cccacttccc attacgttga
     2041 tacaagccac ttgctgaatc tagagctgac agatcatctt gatcaatatc gtttctttga
     2101 atcactatct ggggtgattg ttctgctgtt gaaaagagta gcatctgacc gttcttatct
     2161 cggacagtta cttctaattt tagttttata tcattaagtt gagatagttg acattggtag
     2221 gaaatgcatt ttctacctat accttgtgtt ggataatcta aagaattgtc gttgtaaacc
     2281 caaatgtttc tatcaatatc cgttaaagaa aaggcaatgt aggtaaaatc atcttttaaa
     2341 acttcataag aaacttcaaa tgtgattgtg tcatttggtg ttattcttgg atttgagatt
     2401 aatgacactt tcaaatcttt ggcaatatca gatacagata ttttttcagc attcattgca
     2461 tcctcagctg tctctgtatt atctacacta tactgattag caacatcaaa tggttctcca
     2521 tatgctttaa caagaccatc ttcaataaga actgcacggt tacaatactt cttaacagct
     2581 cccatatcat gtgtcactaa aatagtcgtc ttaccactgt cttttctttc cataaaataa
     2641 tcattacatt ttcgctgaaa agcttcatct cctacagcaa gcacctcatc taaaataaga
     2701 acgtcacctt gggctttaat agccactgaa aatgctaaac gtacctgcat gccagaagaa
     2761 taatttttta atttttgatt cataaaatga tgcaactctg caaaatcaac aatatcattg
     2821 tacatatcat caacttcgtc tttggtaaat cccagcatgg cgccgttcat atagacgttt
     2881 tctcgtcctg ttaattctgg attgaatccc actccaagct ctataaaaga aaccatcttc
     2941 ccatctactg taacctgtcc tttttctgga acatagatct gggaaataat tttaaggagc
     3001 gttgattttc ccgagccgtt acgcccaaca attccaaaaa aatcgccttt ataaacatca
     3061 aagttaatat cttttagcac atgttgttca gtaaatcctt taatccctct taggcgatta
     3121 accagagtcg tgcgaaaact tttagttgct tctgtaggca gtttaaacgt tttactaaca
     3181 tgctgtactt ttactgcaat ttctctatta atcattaaat aatctccgca aatctatcgg
     3241 catttttctt aaagacaaag atgccaataa ataatataac aaatggtact aaatatggaa
     3301 taacaatgta aaaccaattg gttgacatct gccaaatcgt tacgttggcc ctgtcaatca
     3361 ataaataacg aaaatcttga atcatttgtg ctactggatt tagcatcaac aactttgccg
     3421 ccaaagggtg gctatctaaa acaaaagtga tcggataaat gattggagtg gcatacatac
     3481 ctgcttgtaa tagtacttcc cagacttgag ctaagtcacg ataataaaca aagacattcg
     3541 acaataacaa tgcaattcca agcactaaaa caactaattc tataaaaaga aaaagagaga
     3601 gataagcata ccctgatata gtcacaccat ttatcaacgc aaaaattaaa acaacaacca
     3661 aattaataag aaaattaatt aaagctccta acactgccga aaaaacaatg atgtgcttag
     3721 aaaagttcaa ttttcgcaac aagtctcccc gagatacaat agataccatt cccatgctag
     3781 ttgcttctga aaaaaaagac cagatgacat ttgccaataa aagcgctact ggaaaatgag
     3841 gtacatttcc acccaggcga agaaaacgaa taaataccaa gtacataatc gtaaacatca
     3901 taagcggttt taagatagac catagatagc caattgcgct cccttgatag cgtaatttaa
     3961 aatccgtttt taccatttct ctcagtaaaa tacggttttt ctttgttaaa aaattcattc
     4021 ttactttctc ctgtaaccaa ataaggtaac aactaaacta cgaaaaataa aggtgtgaaa
     4081 gatgcgattt tttctaaaac cgtacctttt caaggtagct aagcgtttgg taaaaggcat
     4141 atccaataaa gaaacatagg ccgttaccaa ttcatggtca tttggtttta atggtaaatc
     4201 taatagtaat tgagcctgtt tttggcttga agtaatcaac caccaatatt tgttgactaa
     4261 atgatggggg gttaaccaat ttttcatgcg tttagaccaa gttctagctc ccaacacgtt
     4321 agcatcgtgc tgacggtata attctgttgg aatatcaagg taaactaatt tcccagtagc
     4381 agaagctagt aaagctaaat accagtcatg catcaataaa ccgtcacaag tggtccattc
     4441 ctcagccaga gcatgagtaa tcatcattgt gccgcctgtt acggtatttt cggttaattc
     4501 ttgaagtagg cttgtgtttg catgaccaga ttgagtctta atcatactgt catggcaaat
     4561 agctaaatgt tgggttacca ctttgaggtc tgtataaacc aataaaggag ctgtcatttc
     4621 atgtttttga gcctccaata aggtcacttc cagtttattg tctaaccata tatcatcttg
     4681 gtcactgaaa aaatagacat ctgctttttg gtgctttaag agggtgtaaa aattcttaat
     4741 aactcccaga ttttctgtct gcccctcatt gatccactga attcgtttgt cttccttgac
     4801 aaatgtgcga ataatatcct gggtgccatc agttgaacca tcatctctaa tcaataaggt
     4861 ccagtcgtta actgtttgcc tttggatact ttgaatttgt tctgctaaga agcgttctcc
     4921 attgtaggtg gatagtaaaa tattaatgtt catttaaaaa taaagcctcg tactccccta
     4981 caattttttc ccaagtgtaa tgttcttgaa taatagcttt ggcttctttg cccagatgat
     5041 cactatcaaa acctgcatca acttggttaa tcagctctgc taattgccct ttttgcttgg
     5101 accaatacag cgcagcagac ttagccaccg actggttaaa atcaactcct aaaaccaaat
     5161 taagattggt atgggctagt gcttctagaa ggcctggatt agtccctcct acctcatgac
     5221 catggatata ggcataagcc tgttctcgaa tgtaggctaa tagttctttg tcataaaggg
     5281 taccaacaaa tttaatgcgt gggtctttat cacactctgt ctcagcaagt aattgcttaa
     5341 agtaagcatt gccttcatga ttacaaataa tcactaaatc acgcttagtt gaggaagcca
     5401 taaattcttt aatagcggtt tcgtaattat tttcaggaac aaagcgtcct aaaatgagat
     5461 agtaatcctt ttcgctaata tcaaaggttt gaaaataagc tctgaccttg ctatctgctg
     5521 ttgtcaaacg tgaaggttgt gtctgcgttc cataagcaat aaaacatgtt ttagaccaag
     5581 gataaacctg tttaaggtag cggtcaatgc caatattatc tgaaatgacc aaatcagcct
     5641 gtctagtcat ttgcttttcg gaaaacttca ggtaggcctg cactggtttt gaccatttag
     5701 accgtttcca ctctaacccg tctggattga taaaaaagcg tccacctcga ttgtgaatct
     5761 gcttcacaaa tggcgcaata aaagcgccta cagtattgcc caacacataa aaaatagggt
     5821 tctgaatctg gtgttggtcg ctataagaca gggcataggt aattgccatc atatcataag
     5881 caataactct agcaggacca agttttgggg gattaaggta aaagcaatct gcccccttat
     5941 aatcaaaatg aaccttatgc ttcgtatcgc ttaaacaagc aacgtgataa cgaatgtttt
     6001 tagatgattg atgactaatc aactcttcga caaaggtttc aaagccaccg tatttcgctg
     6061 gtaaccctct gctaccaatg ataaaaacat cctgcattgc atactacccc attctaaaat
     6121 ataatcagtg atattatacc acaattagaa ggctaatgcg accttaaacc catgaaaaag
     6181 agttggcctt agaaagccaa ctctttttag aatacttact ttttcagtcc ttgttggtaa
     6241 aatgccttca aggcttcttg ccatgttgga ataacaaacc ctgttgcttt tgccttatca
     6301 agattcatgg ttgaatttaa aggacgtttg gcttttgctg ggaaagctga tgaatcaact
     6361 ggcaccactt caacagcctt gtcttttaag atttctttgg caaaatcata ccaagtggtg
     6421 tcttccttag catcatttga caagtgatag taaccaaagg ctttttgatt ctctgtcaaa
     6481 taacacatga attctgccaa ggtccttgtc cacgttggcc gaccgtgttg gtcattgaca
     6541 acagtcaaac gggagtgatt ttctgctaac tgctccatgg taaagacaaa gtttttacca
     6601 taatttccaa atacccaagc agtacggata atataaaaat gctctgcgta tcgttcaaca
     6661 gctagttctc ctaaacgttt tgtacggcca tattctgtct tagggtctgg atgatctgtt
     6721 tctacccatt cttgaccgac tggtttattg ccatcaaata cgtagtctgt tgaaatgtaa
     6781 acaagggtag ccccatattt tccacaagcc ttggcaatat tttctgagcc tgttacatta
     6841 atagcctcat tcaaggcttt tccttcgtct tctgctgcat caactgccgt ataagcagca
     6901 caatgataga ccaatgttgg cttgacctgc gcaaaaacag cttcgacttt gtcttcattt
     6961 gtgatgtcca tttctgcgac atcaacggct acataatcaa cacctcgttc atctaataag
     7021 tatcgaagtt ctgtccctaa ttgaccattg cttcctgtaa ttaaaatcat cagttctcct
     7081 ttttgccata ggtagaacac ttttctcagt atcttagtgt tctctaacat taaccaaagg
     7141 ctttcacctg tatttccttt tattctacat caaaaaaggg aatatttcat ccctttacta
     7201 tcaatgatta gcgaataccc agcgcaatcc tagcatagcg gctcatttta tcaaccgtcc
     7261 aagcagggta ccagactaat ttgacctcag tcttagtgac ttctggcaca tcttgcaggg
     7321 catcatggat gtaatctgtc aacaagtctg ccaaaggaca acccattgtt gtgagtgtca
     7381 tatcaatctc tgtataaccg ttatcattaa accgaatttc ataaatcaag cctagattaa
     7441 caacatcaat acctagctcg ggatcaataa cagtttctaa agcttcaaga attctattct
     7501 tgatagctat cacctgatct tgtgtatatt ttggtgtatc agacatggct ttcctttcat
     7561 tttttgaata tgtcgtagat aagtaaaaaa accactaaag ctttgcctat ttagaggaat
     7621 tgccctctgg gcttgtttaa gatatcaagt ctacccttaa aaatacccac cacattcggt
     7681 ccgatttcta atttgtaggt gtaccgctga taagtgccac ttgggatttt cgctcctttc
     7741 ttatttcaaa ggaacgacct aaaatcgtcc actggacgat tttagtcctc tataaagtct
     7801 cttaattgtt tactacgact aggatggcga agttttctaa gggctttagc ttcaatctgt
     7861 cgaatgcgct cacgagtaac attaaagact ttacctacgt cctctaacgt acgcatttta
     7921 ccatcgtcaa ggccaaagcg taggcgaaga acgttctctt cacggtcagt aagcgtatct
     7981 aaaacttcat ccaattgctc acgaagaaca acacgcgtcg tatagtctac tggattttca
     8041 ataacttcat cttcgataaa atcacctaag tgactatcat cttcttcccc aattggtgtt
     8101 tctaaagaaa caggttcttg agcaattttt agaatttcac gaaccttgtc tggagtcatt
     8161 tccatacgtt ctgcgatttg ttctggcgtt ggatcttgac caagttcttg aagaaggtta
     8221 cgttgttcac ggactaattt attaatcgtt tcaaccatat gaacagggat acgaatcgta
     8281 cgtgcttgat ctgcaattgc gcgtgttata gcttgacgaa tccaccatgt tgcataagtt
     8341 gagaatttga atccttttga ataatcaaat ttatcaacag ctttcatgag tcccatgtta
     8401 ccttcttgaa ttaaatcaag aaattgcatt ccacgtccaa cataacgttt tgcaattgag
     8461 acaacaagac gaaggtttgc ttccgctaaa cgttgtttag ccattaaatc tcccttggca
     8521 accgcaacag ccaattcttt ttcctcttca cttgttaaaa gcggtaccac accaatttct
     8581 ttgaggtaca tgcggacagg gtcgtttact ttagctgaat tactaccaat taattcttca
     8641 tcagttaatt cttctggttt tggttcttca acaatgtatt ttgatgatgg gttaccttct
     8701 ttatccgtaa tcgagatacc accatctgtt aaccgctcaa gtaagtcatc aatctgatca
     8761 gcatccaaag caaatggaat gacaagtttt tcggtcacgt catcatcaat cgctgttcct
     8821 tcttttttgt gatggcgaat aaactccgca acttgaacgt taaaagttgt tatttctttt
     8881 tgttttgtca taatttcctc tattccattt ttcgtttctg cgcaataaaa tgttctagta
     8941 cttctagagc cgcttgatga tcgcctttgt tactagattc tctaactttt ttcccttgtt
     9001 tgtgaagatc gcgctctgct aaaagtttgg cacgtttgga taaaatatca tcaatctcac
     9061 caagagctac ttctttggga aggttttctt ctaaaacatt gtaataagca cggttaactt
     9121 cctctgacat ctctgacaaa tcataagatg taatgtgtcc ttgttgcttc agccgttgat
     9181 aaagtaattc taaggtagag gtatcaaaat aaaaatcatc acgatgtcga aattcattta
     9241 ataaatagtc atgatgtaag agccgatgca tgagatgact ttctgtctta gcaatagctg
     9301 tcaaactttt tggcattggt aaggtcacaa gattgctgac agcttgacct tgacgctttt
     9361 gcctatcttg aatccttaat gcatttactg attgttctac ttgaaaatag tcaaagtttg
     9421 gcaacaaatc agcaatcttg ttaatatacg aatgttgagc tgtgatggat ggtgattgag
     9481 caatcaatgg tgccattttc tccacataaa caatttgtga ttgcaaattg tctacattag
     9541 taggttttag gtaatcaata aaaaattcaa cactactgat ccgtgactgc ttaagcaaat
     9601 ctgcaaatgc ttctggggaa tgccgttgta caaattcgtc aggatccatt ttattgggga
     9661 ttctgacaat ttcgacaaca aaatctttaa gcaattctag tgattttgca atagcatgtt
     9721 gtccagcatc gtcaccatca taaatcaaaa caactttttt agtgacttgc ttaaggtgat
     9781 tgacatgttc ttgagtcaat gccgtcccca ttgaagcaac agcattttca tagccggaac
     9841 ggtaagcggc aatcacgtcc ataaaccctt ccattagaaa cacttcatgg gttttggcaa
     9901 taacaggcct tgccttgtcc agatgataca attcataaga tttgttaaaa agaactgttc
     9961 ctcttgaatt tttatacttg gcctgtctct tttccgtatc agctgccgtc cagatacgtc
    10021 ctgaaaaggc aataatatgc cctcggtcat ctgataaggg aaacatgata cgatttcgaa
    10081 aggcgtcata aatggtattg gattgatcgg acaagtgaaa caatcctgaa gcaaccaatt
    10141 gaccttcctc gtatttttta gaaagagctt gataaagata atctgactca tctggggcta
    10201 aaccaatatt gaaatgctca attaattggt catccaagcc tctctggtaa aggtacttcc
    10261 tagcttcttg accaatggta gtggtcatca aaactgcatg gtaaaattta gcagcatcct
    10321 catgaagtgt catcaaagcg tgattagggt gcttgtgttg gctagcaagt actgcctgac
    10381 ttggcggtat attaagcgac ataccagtct tatcggcaat aatctgaaca ctttctaaga
    10441 aggggacttg gcggtattcc tcaataaatt taaaaacatc ccctgatttt ccacagccaa
    10501 agcagtgaaa aaattgtctg tcttcaacaa cattaaaaga gggtgtcttt tccttatgaa
    10561 atgggcaaag cccgagataa tgccgccctg atcgggaaag tttgaccact tctccaatga
    10621 catcgacaat attaacgcta ttttttactt gggaaatcat ttctttgtca attgccaaat
    10681 cgtcacctcc ccataaaaat cccattttag ttttacgttt tattatacca ccgaaactct
    10741 tttttgtaaa tttaaaaact gaagtgattc cattggattt tgtttactaa ttatgctatg
    10801 cttaacttag agatattaaa aagggggaat gtttaatggt taaagaatta aaagcatttt
    10861 tgtttagagg aaacattatt gaa
//
LOCUS       DAUCWU010000053        10832 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUCWU010000053 DAUCWU010000000
VERSION     DAUCWU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.053"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            229..552
                     /locus_tag="VGY24_001232"
     CDS             229..552
                     /locus_tag="VGY24_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284212.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            609..1004
                     /locus_tag="VGY24_001233"
     CDS             609..1004
                     /locus_tag="VGY24_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ4284213.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTVTAIHVGPGQVVNPGDGLITIG"
     gene            1049..2170
                     /locus_tag="VGY24_001234"
     CDS             1049..2170
                     /locus_tag="VGY24_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ4284214.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KIATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            2358..2666
                     /gene="citD"
                     /locus_tag="VGY24_001235"
     CDS             2358..2666
                     /gene="citD"
                     /locus_tag="VGY24_001235"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEQ4284215.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGIDQYDWKEIDSWNV
                     "
     gene            2654..3541
                     /gene="citE"
                     /locus_tag="VGY24_001236"
     CDS             2654..3541
                     /gene="citE"
                     /locus_tag="VGY24_001236"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEQ4284216.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDII
                     DVEAVIERVERENGIEVGRTRMMAAIESAEGVLNARDIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTKKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            3544..5076
                     /gene="citF"
                     /locus_tag="VGY24_001237"
     CDS             3544..5076
                     /gene="citF"
                     /locus_tag="VGY24_001237"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ4284217.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKKKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            5069..5647
                     /gene="citX"
                     /locus_tag="VGY24_001238"
     CDS             5069..5647
                     /gene="citX"
                     /locus_tag="VGY24_001238"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEQ4284218.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            5662..7056
                     /locus_tag="VGY24_001239"
     CDS             5662..7056
                     /locus_tag="VGY24_001239"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ4284219.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREVGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLVDKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVITCRPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(7166..8218)
                     /gene="citC"
                     /locus_tag="VGY24_001240"
     CDS             complement(7166..8218)
                     /gene="citC"
                     /locus_tag="VGY24_001240"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ4284220.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYTLGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAVVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWELLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(8307..8789)
                     /locus_tag="VGY24_001241"
     CDS             complement(8307..8789)
                     /locus_tag="VGY24_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ4284221.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            complement(9489..10559)
                     /gene="xerS"
                     /locus_tag="VGY24_001242"
     CDS             complement(9489..10559)
                     /gene="xerS"
                     /locus_tag="VGY24_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ4284222.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT         3315 a         2136 c         2125 g         3256 t
ORIGIN      
        1 ataataatga tgaacctagt tagatcaagc ttttcttgct atcagtatct tgatctcagt
       61 ttttcaacag ttacctaaaa ataaaataaa aggtaactgt tgaaaaactt tatgaaacat
      121 tgaaagcgct cttagaaaga acacaaaaaa gccatctcaa aaaagattgg tagtatataa
      181 taaacttact agttccctaa ataggtaaat gtgaaaaaga ggtagctcat gcagttttta
      241 caaaaatggt ttaaacactc taaaaaagag aagttgaaag aatcctcgcc ccttagtaca
      301 gaaatagaac cttctgaaaa ctgggaaaag attcccgctt atattcctgc agacaaaagt
      361 gattacaaaa aggttacttt aattaccagt gctattgctg ctggcgatag acctaatagt
      421 caattcaagg ttaaacgcat tctcaagcgc aatcctgagg caatcactgt ttctttaatt
      481 gcttcaagta tcgctgcagg tgtttaccca gaaagtcagt ttcgggtgac atccatctat
      541 tgcaaaaggt agtcggtgaa cgatacttta gacgaactca tgacacaata acatatagga
      601 gaaaaatgat gttacgaaaa tttaaaatta ccattgacgg taaagaatat cttgtagaaa
      661 tggaagaaat cggtgctcct gcccaagcag ctgcccccgc tcaaccaata tccactccag
      721 tacctgtccc tactgaagca agcccacaag tggaggaagc gcaagcacca cagccagtag
      781 ctgcagcggg agcggatgcc attccatcac caatgcctgg aaccatctta aaagtcttag
      841 tagcagtggg agaccaagta actgaaaatc agccactatt gattttagaa gccatgaaga
      901 tggaaaatga gattgtggcc tcatcagcag gaaccgttac agctatccac gtcggtccag
      961 gtcaagtggt caatcctggt gatggtctca ttacaattgg ttaatagtac tataactaga
     1021 ataaatagac agaaaaggaa gtgaatttgt ggacacttta atcgcaggaa taacctccat
     1081 caccattcct caaattgtta tgatggtcat cggtgccctc ttaatgtatc taggaattaa
     1141 aaaagaatac gaaccaacct tacttgtccc catgggactt ggaaccattc tcgttaactt
     1201 ccctggttca ggagttttaa cccaagtggt taacggagtc gagcaagaag gggtttttga
     1261 ggctctcttc aattttggga ttggaacaga acttttccca ctgttgattt tcattggtat
     1321 aggagccatg attgactttg gtcccttgct tcaaaaccca ttcatgctct tgtttggtgc
     1381 tgcagctcaa ttcggaattt tctttgttgt cgttgttgct gtactggctg gctttgacat
     1441 taaagaagca gcttcaatcg gcattatcgg agcagcagac ggaccgactt ctatctttgt
     1501 tgccaaccaa ctggccaagg atttactggg tccaattacg gttgcagcct attcttacat
     1561 ggctctggtt cctattatcc aaccatttgc cattaaatta gtgacaacga aaaaagagcg
     1621 ccgtatccgt atgacttaca aagctgagaa tgtatctcaa atgactaaaa tcttattccc
     1681 aatcatcatt accttagtag cagggttcat cgccccaatt tctctacctt tagttggctt
     1741 tttgatgttc ggtaacttat tacgtgagtg tggtgtgctc gatcgcctgt cacaaactgc
     1801 tcaaaatgaa ttggtaaaca ttattagtat cttgcttgga ttgaccatct ctattaaaat
     1861 gcaggcagac ctgttcttaa atgtacagac actcttaatc atcgtctttg gtttacttgc
     1921 ttttatcatg gattctatcg gtggggtcat gtttgccaaa tttctcaatc tcttccgaaa
     1981 agagaagatt aatccaatga ttggcgctgc gggtatttca gcttttccaa tgtcaagtcg
     2041 tgtcattcaa aaaatagcaa cagatgaaga cccgcaaaac tttatcctca tgtatgctgt
     2101 aggtgccaat gtctctggcc aaatcgcctc tgttatcgca ggtggtttgt tgctagctta
     2161 ctttagctaa gaaaggaagg aaaccttatg acaattaata tggactattt gattagttcc
     2221 tttgagctaa tggcgcttgg tatggcaggt gtctttatcg tgttaggcat tctctatctt
     2281 gtcgcagaga tcctgatcaa acttttccca gttagtaaat aacaatatca tttaagaaaa
     2341 tcaaggaggt aaacgcaatg gatattaaac aaactgccgt tgctggttca cttgaatcaa
     2401 gtgacctaat gattacagta tcccctaatg acgagaaaac tattacaata accctagaca
     2461 gtagtgttga aaaacaattt ggcaatcaca ttcgtcaact cattcatcaa accctagtga
     2521 atttgaaggt gacggctgct aaagtagagg ctgttgataa aggtgcatta gattgcacca
     2581 ttcaagcgag aaccatcgca gctgttcatc gcgctgctgg tattgaccaa tacgattgga
     2641 aggagattga ctcatggaac gtttaagaag aacaatgatg tttgtgcctg gtgccaatgc
     2701 agccatgctt cgagatgctc ctttgtttgg tgctgattca atcatgtttg acttggaaga
     2761 ttctgtttca ctcaaagaaa aagacacttc tagagctctc gttcattttg cgcttaaaac
     2821 ttttgattat tcaagcgttg aaacggttgt tcgtgtgaat ggtcttgatt cttgtggggc
     2881 tttagacatc gaagctgttg ttttggcagg tgtcaacgtg attcgtcttc caaaaacaga
     2941 aactgctcaa gatattattg atgtggaggc tgttattgaa cgtgtcgaac gcgaaaacgg
     3001 cattgaagtt ggtcgcacac gcatgatggc agccattgaa tcagccgaag gtgtcctaaa
     3061 tgctcgtgac attgccaaag cttctaagcg cttgattggt attgctcttg gagcagaaga
     3121 ctatgtcacg aatatgaaaa cgcgtcgtta cccagatggt caagaattat tctttgctcg
     3181 tagcatgatt ttacacgctg ctcgtgctgc tggaattgct gccattgata ctgtttattc
     3241 tgatgtcaat aataccgaag ggttccaaaa cgaagttcgc atgatcaaac agttaggatt
     3301 tgatggtaaa tcggtcatca accctcgcca aattcctcta gtcaatgaga tttatacccc
     3361 aacaaaaaaa gaaattgacc atgccaaaca agtcatctgg gcaattcgtg aagctgaaag
     3421 caaaggctct ggcgttattt ccttaaatgg aaaaatggtt gataaaccaa tcgttgaacg
     3481 tgcagaacgt gtgattgccc tagcaacagc agcaggtgtt ttatctgagg aggatattta
     3541 aagatggtaa caaataaact cggtagagat attcctcaac catacgccga ccaatacggt
     3601 gtctttgaag gagaactcgc aaatatcaaa cagtatgacg aatcaagtcg ccgtattaaa
     3661 ccagttaagc ctggagattc taaattacta ggatctgttc gtgaagcgat tgaaaaaaca
     3721 ggtttaacag atggcatgac catttctttc caccaccatt tccgtgaagg ggatttcatt
     3781 atgaatatgg tcttggagga aattgccaaa atgggcatta aaaacctgtc tattgcccca
     3841 agttccatcg ctaatgtaca tgagcctttg attgatcaca tcaaaaatgg tgtggtcact
     3901 aacatcactt catctggtct tcgtgacaaa gtaggggcag ccatctcaga aggactaatg
     3961 gaaaatcctg tggttattcg ctcccatggt ggtcgcgctc gtgccattgc tagtggggat
     4021 atccatattg atgttgcctt tctgggcgcc ccaagttcag atgcttacgg aaatgttaat
     4081 gggacaaaag ggaaagcaac ctgtggttct ttgggctatg ccatgattga tgccaaatat
     4141 gcggatcagg tggttatctt gacagataat ttggttcctt atcctaatac cccaatcagc
     4201 attcctcaaa cagatgttga ctatgtggta acagtggatg ctatcggaga tcctcaagga
     4261 atcgccaaag gggcaactcg ttttacgaaa aatcctaaag aactcttaat tgcagaatac
     4321 gccgctaaag taatcaccaa ctctccttat ttcaaagaag gattctcttt ccaaactgga
     4381 acaggtggcg cttctttggc agtaacccgt ttcatgcgtg aagcgatgat taaagaaaat
     4441 atcaaagcta gctttgctct tggtggtatt accaatgcta tggtggagtt gctcgaagaa
     4501 ggactggttg aaaaaattct tgatgtccaa gactttgatc acccatcagc tgtttctctt
     4561 ggtaagcatg ctgaacatta tgaaattgat gccaacatgt atgcctcacc tttgagcaag
     4621 ggtgctgtta tcaatcaatt agacacttgt attttatcag cccttgaagt cgatactaac
     4681 ttcaatgtta atgtgatgac aggatctgac ggcgtgattc gtggcgcttc tggaggacac
     4741 tgcgacactg cctttgcggc taagatgagt ttggttattt caccacttat ccgcggacgc
     4801 atcccaactt ttgtagatga ggttaatacg gtcattacac caggaacaag tgtcgatgtg
     4861 gtcgtcacag aagtgggaat tgccattaac ccaaatcgtc aagacttagt agaccatttc
     4921 aaatccctca atgtgccaca atttagtatt gaggagctaa aaaagaaggc ttacgccatc
     4981 gttggcacac ctgagcgtat tcaatatggt gataaggttg ttgctcttat tgaataccgt
     5041 gatggtagcc tcatggatgt ggtttacaat gtgtaaagac acttattttt caggcgaagc
     5101 tatccaactt agtgatatgt taagagcccg cgaagaaaga gctctgcgtc agctgcattt
     5161 attaaaggag tacccagaag gtagcttatt atcggtcacc atgaatatcc ctggaccaat
     5221 taaaacctct cctaaacttc ttgaagcttt tgatatagtg attaaggcca ttcaaactgc
     5281 cttagctgac gataagattt gttaccagtt gcgattactg cctacaacgg gttatgagta
     5341 ttacctcatc acaagtctac ctagccgcga cctgaagtta aaaatgatag ccttagagac
     5401 agagttgcca ataggtcgtc tcatggattt agatgtcttg gtcttgcaaa atgatctgcc
     5461 tcattcaatt agcagaaccg tattaggagg ctcccctagg caatgtttta tctgttctaa
     5521 agaggctaaa gtctgcggtc gcctacgtaa gcacagtgtc gaggagatgc agactgctat
     5581 ttcaaaatta ctccattcat ttttcaataa agacaaccaa tcatcgtcat cagataagac
     5641 aggttgatga ggagaacaat tatgacgaaa atccgtataa cggaaacagt tttacgtgat
     5701 ggtcaacaaa gtcagattgc cacacgtatg acaaccaagg aaatgattcc tattctcgaa
     5761 acgctagaca atgctggtta tcatgccctt gaaatgtggg gaggagcaac ctttgattcc
     5821 tgcctacgct ttttaaatga agacccttgg gaacggctaa gagccatccg taaagcagtg
     5881 aaaaagacta aacttcagat gctcttacgc ggacaaaacc ttcttggata tcgcaattat
     5941 gctgacgatg tggtcagatc cttcgttcaa aaatccattg aaaacgggat tgatattgtc
     6001 cgtatcttcg atgctttaaa tgaccctcgc aacttgcaaa cagctgtttc agcgactaaa
     6061 aaatttggag ggcatgctca agttgccatt tcttacacga caagtccggt acataccatt
     6121 gactactttg ttgaattggc gaaagcttac caagctatcg gagctgactc catttgtatc
     6181 aaagacatgg ctggtgtctt aactcctgaa ataggttacc aattggtcaa atgtatcaag
     6241 gagaatacaa ctatccctct tgaggttcat acccatgcta ccagtggtat ttcagaaatg
     6301 acttacttaa aagtagcaga agcaggagct gatattattg atacggcgat ttcctctttt
     6361 tcagggggaa ccagtcagcc tgccacagag tcaatggcga ttgccttgac agatttgggc
     6421 ttcgacactg gcttggatat gcaagaggtg gccaaagttg cagaatattt caacactatt
     6481 cgtgaccact atcgagaagt agggatttta aatcctaaag tcaaagatac tgagccaaaa
     6541 acattgattt accaagttcc aggtggtatg ctatcaaacc tattgagcca attaaccgag
     6601 caaggcctag ttgataaata cgaagaagtc ttagcagaag tacctaaagt aagggccgat
     6661 cttggatatc caccacttgt aacgccatta tcacaaatgg ttggtacaca ggccttgatg
     6721 aatatcatct caggggaacg ctacaaggta gttccaaatg aaatcaaaga ctatgttcga
     6781 gggctatacg gtcaatcacc agccccacta gcagaaggta tcaaagagaa aatcattggt
     6841 gacgaagcag tcattacttg cagaccagcc gacctaatcg agcctcaaat gatttatctg
     6901 cgtgacgaga ttgctccata cgctcattca gaagaagacg tgttaagcta tgcaagcttc
     6961 ccgcaacaag ctagagattt cttgggacgc cgtgaagatc ctttctatga tgttccggtt
     7021 caagaagtta ctgtacaact ggacattcaa gactaacaga gaatgactaa tcctcttaac
     7081 cattaatctc cttggtgaaa cgcatttgct gtaagaacaa taccaaaaac ctacctgatt
     7141 ttgataatca gataggtttt ttatcttagt aatgtttgac agatgaagtg gcttgtatct
     7201 ttttaataat gggttgggct tctagcgaac aaaaataatc taaagacgtt ttgggaagga
     7261 gttttgccag aagttcccaa tcattatctt tcaatgcctg cctagccgtt gaggcactga
     7321 taggatcttg ctgctgattg atagctttcc ttggcaaaat atctaggagg ataccagctt
     7381 gctgcaactg ctctgccata atttcattgt aaatagcagt tactcggctg gttggctctt
     7441 ctccaacgta tctttttgta atctgtaagg tttgagcaat ctttagaaaa atagcagtat
     7501 ccagcaaagc ttggctctta atcacgtcat tgtcttcttt ttggaaatag gctgggaaag
     7561 tcgcttggct aatcaaataa ggacctgttt catgatatat gacattatca agatgagcta
     7621 gaccttcttg aatcaacctc tttctcaccg aaaaaggaat tagactgcgg tcttcactga
     7681 ccataaacag atgaacccaa tcattttctc ttgcagcttt ctctactaaa aattgatgcc
     7741 ctaaggtaaa ggggttagcg ttgataacga cagcagcgac tttaccaggt gtttgctcag
     7801 gcttattaag tgtcatcaga taatcttgaa aaccagtttt tttattttcc aagaaactga
     7861 tgtgattgtc gatatggaca atttcagtaa aaccaagatc tttgaaaaaa gcagcagaag
     7921 aggtcttcgt ataaacaaaa agatgatagt tgccaagggt atattcttca tcaatgagat
     7981 gactcacaat cctattaaga agtccttcac cttgataggc actggatacg caaagacagc
     8041 gcaagctatt tccaaagtaa gaaccggtag caatcacatc attttgcgca ttcatgatgg
     8101 cacaagtata atctaaatga gcatccaaac gaataccttc ttgatgaagt aggttcttta
     8161 cactagccat tgtcgtttta tctgaaggaa agacttttga aatagtgtag taaggcattt
     8221 cttctcctta tctttaattt tattgaatca taacataact tagtaccaaa acaggctgat
     8281 ctatctaaag gcaactctgt tttttatcaa cgccattttt taaaaggaaa tcgaatgtgt
     8341 ttgacagact caaaagtatc agcgattttt tgattgcgtt ccacagtccg caagttttct
     8401 tggtctaata agtcttgact tttttccaaa acatcaacag taatgtagta gagtttgatc
     8461 aggcttccta tcttatattt accagatttt ggagacatag caaccacttc attgagatta
     8521 ctatgaagcc attttttatg aggcttagca ggtatagtgg ctactacaaa gccaagttgt
     8581 tccaaatgag cttgtgcctg atcaacagga agattaatca catcatctat cgtgcgtagt
     8641 tgcatctctt tttcatgttt ttgttccata cgtttttcga taattggtcg actgttatca
     8701 atcgtttttc caataatttc agtagtgtca ggaacaattg acatcagttt cccaactact
     8761 cctagagctt ttcttattct ctttggcatc ttttatctcc tcaagtcgtt tctttttatt
     8821 ataacaaaat gtctaaaaat agtatgcgat aaaccttgtt ctaaaaggtt aaatagatca
     8881 attcaaggaa aaaataggag gcgatccgac aaaaaaacac aaaaattcta aaaaagtcaa
     8941 gctattttag tttagccttt gtcaatcgta tcagttcaaa tagaaaatac aagttttgac
     9001 aagaatttat ttatgtaata aactgtgttt ttgattcttc agtctatcta gcgataatgt
     9061 tatctgattc ttgttttcaa ccaattgatg ttaaagaagc tgtcttcatg attctctaat
     9121 attccttcaa ttttagcttt atattactct aagttatcag taatgacatt aagtagttta
     9181 acaagaataa tcctaaaagc tactttacaa gataacatat tatcatacta aataaaagta
     9241 ctgacatact aaaaacaggc ttatctcatc tttttgatga gaaccctact agatttaatc
     9301 tataaacatc gcaaaaaagt cattcaaaga agggacaaag ttagcagtga tattcctttt
     9361 tctaagtgac ttgagtagtt tggaaaacga cccgacacca aactgtcacg gttaatagta
     9421 gccatctatt aaagttaatt ttgaagctat ttttatcttt aatgttacat aaaaacaatt
     9481 atgtaacatt aaaggttatc taaagcattt ttttgctcat cattgacaat atgggtataa
     9541 agatccgtga cttgagtaga ggaatggcct aattgatgac taactaacac ctgagattta
     9601 gtagcatcat atagtcttgt cgctagcgta tgacgcaatt tgtgaggagt cacacgaatt
     9661 ttaaaatcct cagagtactt gccaaccatc ttttctatac tagaagcatc cattcgattt
     9721 ggaacgccac ggtattctgt taaaaagaaa gcaagatcct gtttttcagc cttgtaacgt
     9781 ctctgacgaa cagctagata agactccaaa taacctttcg caaaacctgc tacattgact
     9841 gaatcacgtt ttccgccttt gcgaataact tcgataatca tcatatttag attgacatct
     9901 tttaaatcaa gattcactgc ttcagacaaa cgaacaccag aagcaagtag tagagcaata
     9961 atagccaaat ccctctcctt atttttacgg aaagaagatt tggcacgatt agacaatttt
    10021 tgctcatatt ccttatccac ataatccaag aaagctagag tttcatcacc taaaaacagt
    10081 ttctgcttaa tattttccgc tctggaagcc agggtttctt ttttcttttt tgttgacact
    10141 tttttcataa catttcgata aaaataaggc tccccttggt cattttcaac ctcttcagtc
    10201 aagtacttgt aaaggcttga tagggcagac aaggtccgat taatagtggt ttgagaaaga
    10261 ccctctttgg tggaataggt gttgagagaa ggtcgttccc gcaaataaag cacaaaggcc
    10321 tctaaatcct tcttggtaag gtgctcaagc gtgctcaagt caatatcagc aatctttggc
    10381 gcctgcgtta aatcagcatc catcagccaa tcaaaaaagc gtttgtattc ttttaaatac
    10441 tcatataagg tggtaaagct gtatggaaca gctaatttgg attggtaata atctaaaaca
    10501 taccaaggca tgatagcctt gtaggtttca attttttcta gtaataattc gcgtctcata
    10561 tcatcataat tccttaacaa taacaatttt tcaaaaatga attttcgcaa attgtctcat
    10621 gtattttatg ccattcattt tctacaagag cagtatatca cacctataaa tatttttcaa
    10681 gaaaattata gtttttcgga aagatgtcac gagacaaatc tcttttttta cagatactaa
    10741 cgtcacttct tttagattca gtaataattt actatcatta ttttagtaac ttttaggcac
    10801 agaatttgtt tagctcagaa ctaaatgata at
//
LOCUS       DAUCWU010000054        10804 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUCWU010000054 DAUCWU010000000
VERSION     DAUCWU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.054"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            15..1238
                     /locus_tag="VGY24_001243"
     CDS             15..1238
                     /locus_tag="VGY24_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ4284223.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1297..2103
                     /locus_tag="VGY24_001244"
     CDS             1297..2103
                     /locus_tag="VGY24_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ4284224.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            2336..3691
                     /gene="dnaA"
                     /locus_tag="VGY24_001245"
     CDS             2336..3691
                     /gene="dnaA"
                     /locus_tag="VGY24_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ4284225.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQVQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            3846..4982
                     /gene="dnaN"
                     /locus_tag="VGY24_001246"
     CDS             3846..4982
                     /gene="dnaN"
                     /locus_tag="VGY24_001246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ4284226.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDININVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            5058..5255
                     /locus_tag="VGY24_001247"
     CDS             5058..5255
                     /locus_tag="VGY24_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ4284227.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            5584..6699
                     /gene="ychF"
                     /locus_tag="VGY24_001248"
     CDS             5584..6699
                     /gene="ychF"
                     /locus_tag="VGY24_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ4284228.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDDFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNILQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEGKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            6769..7338
                     /locus_tag="VGY24_001249"
     CDS             6769..7338
                     /locus_tag="VGY24_001249"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ4284229.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            7341..>10804
                     /gene="mfd"
                     /locus_tag="VGY24_001250"
     CDS             7341..>10804
                     /gene="mfd"
                     /locus_tag="VGY24_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ4284230.1"
                     /translation="MDILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKTLAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEVSDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEHEIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQISSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     SDMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYIADERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERL"
BASE COUNT         3723 a         1728 c         1984 g         3369 t
ORIGIN      
        1 tcggaggaca aaacatgcca agtatgaaac atatcttaaa atccttaagt attttattaa
       61 ttggattttt aggaggatta atagcaatta ttacattcaa taatctctac ccacattctc
      121 cttcaaaaat aaattcaggt aaggcaacaa ctagtaatat ggtttttaac aacacaacca
      181 atactactaa agctgtcaaa gctgttcaaa atgcagttgt atcagttatt aattatcaag
      241 ataacccttc ttcatccctt tctaaccctt atacaaaact ctttggagaa gggcgttcaa
      301 aagagaataa ggatgctgaa ttatctattt ttagtgaagg atctggggtc atttatcgaa
      361 aagatggcaa ctccgcttac gttgttacta ataaccatgt tatcgacgga gctaaacgaa
      421 ttgaaattct tatggcagac ggatctaaag ttgttggtga attagttgga gctgatactt
      481 attcggattt agctgttgtt aagatctctt cagataagat aaaaacagta gctgaatttg
      541 cagattctac aaaactaaat gttggagaag ttgctattgc tatcggcagc ccactaggaa
      601 cacaatacgc taattctgtt actcaaggaa tcgtctctag tttaagtcgt actgtaactt
      661 taaaaaacga gaatggtgag actgtctcaa caaatgctat tcagacagat gctgctatta
      721 accctggaaa ctctggtgga ccactaatta atattgaggg gcaagtaatc ggaattaatt
      781 caagtaaaat ttcttctacc ccaactggta gcaacggtaa tagtggtgct gttgaaggaa
      841 ttggttttgc tatcccatct actgatgtta ttaaaattat taaacaacta gaaactaatg
      901 gtgaagttat cagacctgct cttggaatat ccatggtcaa tttaaatgac ttatccacaa
      961 atgctcttag tcaaattaat attccaacta gtgtaactgg tggtattgta gtagcagaag
     1021 ttaaggaagg aatgccggca tctggcaaac ttgctcagta cgatgtgatt acagaaattg
     1081 atggcaaaac agttaattca attagtgatt tacaaagtag tctatacgga cacgatatta
     1141 atgatactat taaagtaact ttttatagag gaactacaaa gaaaaaagca gatatcaaat
     1201 taacaaaaac tactcaagat ttgactaaaa cgcagtaaac aagctaccta aatactatta
     1261 cattttatca cataaaacag tccaaaggac tgttttatgt ttcttattct atccaggaac
     1321 tcccttatga caaaagaatt attgatagac ctaccaattg aagatattgt tacaaatccc
     1381 tatcagccaa ggatacaatt taatcaaaga gaactccaag acctagcaac ttctattaaa
     1441 tcaaacggcc tcattcaacc tattattgtt cgaaaatctg acatttttgg ctatgagtta
     1501 gttgctggag aacgacgact taaagcttca aaaatggctg gtctaaaaaa agttccagct
     1561 atcatcaaga agatctctac actcgagagc atgcaacaag ctatagttga aaatttacaa
     1621 cgttctaacc ttaacgctat cgaagaagct aaagcctatc agttattggt tgaaaaaaaa
     1681 cacatgactc acgatgagat tgctaaatat atgggaaaat caagacctta tattagcaat
     1741 accttacgtc tgttacaact cccagcacct atcattaaag caattgaaga aggaaaaatt
     1801 agtgcgggac acgcgcgtgc tcttttaact ttgagtgatg ataagcaaca actgtacctc
     1861 actcataaaa tacaaaatga aggcctaagt gttaggcaaa ttgagcaact ggttacttct
     1921 actccaagtt cgaagctatc taaaaaaact aaaaatattt ttgcaacttc tttagagaaa
     1981 caattggcta aatcattggg actctctgtc aatatgaagc tgacagcaaa ccatagtggg
     2041 taccttcaga tatctttttc caatgatgat gaattaaaca gaattatcaa caagctaaag
     2101 tagcttgttg atattctgtt ttttcttttt tagttttcca catgaaaaat agttgaaaac
     2161 aatagcggtg tcaccttaaa atggcttttc cacaggttgt ggagaaccca aattaacagt
     2221 gttattttat tttccacagg ttgtggaaaa actaattatt atccatcgtt ctgtggaaaa
     2281 ctagaatagt ttatggtaga atagttctag aattatccac aagaaggaac ctagtatgac
     2341 tgaaaatgaa caaatttttt ggaacagggt cttggaatta gctcagagtc aattaaaaca
     2401 ggcaacttat gaattttttg ttcatgatgc ccgtctatta aaggtcgata agcatattgc
     2461 aactatttac ttagatcaaa tgaaagaact cttttgggaa aaaaatctta aagatgttat
     2521 tcttactgct ggttttgaag tttataacgc tcaaatttct gttgactatg ttttcgaaga
     2581 agacctaatg attgagcaaa atcagaccaa aatcaatcaa aaacctaagc agcaagcctt
     2641 aaattctttg cctactgtta cttcagattt aaactcgaaa tatagttttg aaaactttat
     2701 tcaaggagat gaaaatcgtt gggctgttgc tgcttcaata gcagtagcta atactcctgg
     2761 aactacctat aatcctttgt ttatttgggg tggccctggg cttggaaaga cccatttatt
     2821 aaatgctatt ggtaattctg tactattaga aaatccaaat gctcgaatta aatatatcac
     2881 agctgaaaac tttattaatg agtttgttat ccatattcgc cttgatacca tggatgaatt
     2941 gaaagaaaaa tttcgtaatt tagatttact ccttattgat gatatccaat ctttagctaa
     3001 aaaaacgctc tctggaacac aagaagagtt ctttaatact tttaatgcac ttcataataa
     3061 taacaaacaa attgtcctaa caagcgaccg tacaccagat catctcaatg atttagaaga
     3121 tcgattagtt actcgtttta aatggggatt aacagtcaat atcacacctc ctgattttga
     3181 aacacgagtg gctattttga caaataaaat tcaagaatat aactttattt ttcctcaaga
     3241 taccattgag tatttggctg gtcaatttga ttctaacgtc agagatttag aaggtgcctt
     3301 aaaagatatt agtctggttg ctaatttcaa acaaattgac acgattactg ttgacattgc
     3361 tgccgaagct attcgcgcca gaaagcaaga tggacctaaa atgacagtta ttcccatcga
     3421 agaaattcaa gtgcaagttg gaaaatttta cggtgttacc gtcaaagaaa ttaaagctac
     3481 taaacgaaca caaaatattg ttttagcaag acaagtagct atgtttttag cacgtgaaat
     3541 gacagataac agtcttccta aaattggaaa agaatttggt ggcagagacc attcaacagt
     3601 actccatgcc tataataaaa tcaaaaacat gatcagccag gacgaaagcc ttaggatcga
     3661 aattgaaacc ataaaaaaca aaattaaata acatgtggaa aagaatatct tttatgaaat
     3721 agttatccac aagttgtgaa caaccattta gtcttggatt ctctcgttta tttagagtta
     3781 tccactatat acacaagacc tactactact acttattatt atacttataa aataaaggag
     3841 ttctcatgat tcaattttca attaatcgca cattatttat tcatgcttta aatgcaacta
     3901 aacgtgctat tagcactaaa aatgccattc ctattctttc atcaataaaa attgaagtca
     3961 cttctacagg agtaacttta acagggtcta acggtcaaat atcaattgaa aacactattc
     4021 ctgtaagtaa tgaaaatgct ggtttgctaa ttacctctcc aggagctatt ttattagaag
     4081 ctagtttttt tattaatatt atttcaagtt tgccagatat taatataaat gttaaagaaa
     4141 ttgaacaaca ccaagttgtt ttaaccagtg gtaaatcaga gattacccta aaaggaaaag
     4201 atgttgacca gtatcctcgt ctacaagaag tatcaacaga aaatcctttg attttaaaaa
     4261 caaaattatt gaagtctatt attgctgaaa cagcttttgc agccagttta caagaaagtc
     4321 gtcctatttt aacaggagtt catattgtat taagcaatca taaagatttt aaagcagtag
     4381 cgactgactc tcatcgtatg agccaacgtt taatcacttt ggacaatact tcagcagatt
     4441 ttgatgtggt tattccaagt aaatctttga gagaattttc agcagtattt acagatgata
     4501 ttgagaccgt tgaggtattt ttctcaccaa gccaaatctt gttcagaagt gaacacattt
     4561 ctttttatac acgcctctta gaaggaaatt atcccgatac agaccgttta ttaatgacag
     4621 aatttgagac ggaggttgtt ttcaataccc aatcccttcg ccacgctatg gaacgtgcct
     4681 tcttgatttc taatgctact caaaatggta ctgttaagct tgagattact caaaatcata
     4741 tttcagctca tgttaactca cctgaggttg gtaaggtaaa cgaggattta gatattgtta
     4801 gtcagtctgg tagtgattta actatcagct tcaatccaac ttaccttatt gagtctttaa
     4861 aagctattaa aagtgaaaca gtaaaaattc atttcttatc accagttcga ccattcaccc
     4921 taacaccagg cgatgaggaa gaaagtttta tccaattaat tacaccagta cgaacaaact
     4981 aagtaagaaa aagctccctt ttaggagttt tttttgttat tataaatatt aacgataata
     5041 taagtggaga aaagggaatg tatcaaattg gatcatttgt tgaaatgaaa aaacctcatg
     5101 cttgtgtaat taaagaaact ggtaaaaagg ctaatcaatg gaaggtactg agagtaggag
     5161 ctgatattaa aattcagtgt actaactgtc agcatgtgat tatgatgagt cgttatgatt
     5221 ttgagcgaaa attaaaaaaa gtattgcaac catgaaagtc cttttaaata aataattcta
     5281 gctatatttg caaccaatac tttcctaaaa aattgctagt atgccgttgg aaaattagct
     5341 attctaatgt tatcgaaaga agaaaggtgg ccgttgacaa tgacgaaagt tgtagcacag
     5401 ttacttagtt tctcggcaaa ctatatcaaa atgagaataa tacaccaaac tttgataatt
     5461 taaatgaagt gaaagagtgg cataatgata agcaaatctg aaattttctt cttattccta
     5521 ccgctttttc ttctattttt tggtataata atcttgattg aaatttgaat ggagattcgc
     5581 taaatggctt taacagcagg tattgtaggc ttaccaaatg ttggtaaatc aaccttattt
     5641 aacgcaatta caaaagcagg agcagaagct gctaattatc cttttgcgac tattgatccc
     5701 aatgttggta tggtagaggt tccagatgag cgtctgcaaa aattgacaga attgattaca
     5761 cctaaaaaaa cagttccgac aacctttgaa tttactgata ttgcaggtat tgttaaaggt
     5821 gcttctagag gggaaggtct aggaaataaa tttttagcta atattcgtga agtcgatgct
     5881 attgttcatg tggtacgtgc ttttgatgat gaaaatgtca tgcgtgaaca aggtcgtgag
     5941 gatgatttcg ttgatcctat agcagatatt gacactatta atcttgaatt aatcttagct
     6001 gatttagagt caatcaataa acgttatgcg cgtgttgaaa aaatggcacg aactcaaaaa
     6061 gataaagaat cagtagcaga gttcaatatt ttgcaaaaga ttaaacctgt tttggaagat
     6121 gggaaatcag ctaggacaat tgagtttaca gaagatgaag caaaagttgt taaaggtctc
     6181 tttttattaa caactaaacc tgttttatat gtagctaatg tcgatgaagg taaagttgct
     6241 aatccagatg gtattgatta tgtcaaacaa attcgtgact ttgcagctac tgaaaatgct
     6301 gaagtagttg ttatctcagc gcgtgcagaa gaagaaattt cagagcttga cgatgaggat
     6361 aaagaagaat ttttggaagc tatcggtctt actgaatcag gcgttgataa attaaccaga
     6421 gcagcttatc atctcttagg ccttggaacc tattttacag caggtgaaaa agaggttcgt
     6481 gcttggacgt ttaagcgtgg tataaaagct ccacaagctg ctggtattat ccattcagat
     6541 tttgaaagag gttttattcg tgcagtaacc atgtcttatg atgatctaat gacctacggt
     6601 tcagaaaaag ccgtcaaaga agctggacgc ttgcgtgaag aaggaaaaga atatgttgtc
     6661 caagatgggg acatcatgga attcagattt aatgtgtaat tatattaaaa caataccaga
     6721 aggttggaag aacattccag cccttttggc attttagaaa gagaaaatat ggtaaaaatg
     6781 attgttggtc tgggaaatcc aggctctaaa tatgaaaaaa caaagcacaa tattggtttt
     6841 atggctattg acaatattgt caagaacctt gatgttacct ttacagatga taaaaatttt
     6901 aaagcacaga taggaagtac ttttattaat catgaaaaag tttactttgt gaaacctact
     6961 acttttatga ataatagcgg catagcagta aaagcattac taacctacta taatattgac
     7021 ataacagatt taattgttat ctatgatgat ttagacatgg aagtcagtaa attacgttta
     7081 cgtagtaagg gttcagcagg aggacataat ggcattaagt caatcattgc ccacattgga
     7141 actcaggaat ttaaccgaat caaagttggt attggacgac ctttaaaagg tatgactgtt
     7201 attaaccatg tgatgggcca attcaatacc gaagataata ttgctatttc gttaactctt
     7261 gacagagttg tcaatgctgt caagttttat ttacaagaaa atgattttga aaaaacaatg
     7321 cagaaattta atggataatc atggatattt tagaattatt tagtcagaat aagaaagtcc
     7381 aatcctggca ctctggatta accaccttag gaagacaact ggtaatgggg ttatcgggtt
     7441 caagtaaaac attggctata gcttccgctt atttagatga tcaaaaaaaa atagttgtgg
     7501 ttacatcaac tcaaaatgag gttgaaaaat tagccagcga tttatctagt ttacttgatg
     7561 aagaacttgt tttccaattt tttgcagacg atgtggctgc agcggaattt atctttgcgt
     7621 caatggataa agctctatca agaatagaaa ccctgcaatt tttaaggaat cctaaatctc
     7681 agggcgtttt aattgttagt ttatcaggct taagaacttt attgccaaac ccagatgttt
     7741 ttacaaagag tcagattcaa ctaacagttg gagaagatta tgatagtgat actcttacta
     7801 aacaactgat gacaattggc tatcagaagg tctcacaggt tattagtcca ggagaattta
     7861 gccgtcgagg ggatatttta gatatctatg agattacaca agaattgcct tatcgattgg
     7921 aattttttgg cgatgatatt gatagtatta ggcaatttca tccagaaact caaaaatctt
     7981 ttgaacaact agaaggtatt tttattaatc cagcaagtga tcttattttt gaggttagtg
     8041 attttcaacg tggcattgag caattagaaa aggctctaca aacagcacaa gatgataaaa
     8101 aatcttattt agaagatgta ttagctgttt caaaaaacgg ttttaaacat aaggatatcc
     8161 gtaaatttca atcattattt tatgaaaaag agtggtcatt attagattat attcctaagg
     8221 gaacgccaat cttttttgat gattttcaaa aactagttga taaaaatgca agatttgatt
     8281 tagagattgc taatctcttg acagaagatt tacagcaagg aaaggctctt tccaatctta
     8341 actactttac agataattat cgagagctta ggcactataa gccagcgacc ttcttttcaa
     8401 attttcataa gggacttgga aatatcaaat ttgatcagat gcatcagcta actcagtatg
     8461 ccatgcagga attttttaat caatttcctt tgttgattga tgagattaaa cggtatcaaa
     8521 aaaatcaaac aacggttatt gtacaggtag agtctcagta tgcttacgaa cgacttgaaa
     8581 aatcttttca agattaccaa tttcgccttc ctttagtgag tgctaatcaa attgtttcac
     8641 gtgaatcaca aattgtaatt ggagctatct ccagtggttt ttattttgct gatgaaaagt
     8701 tagcgttaat cacagagcat gaaatttatc ataaaaagat caaacgacgc gctagacgat
     8761 ctaatattag caatgctgag cgtttgaaag attacaatga gttagcagta ggtgattacg
     8821 tggttcataa tgtccatggt atcggtcgct ttcttggaat tgaaacgatt cagattcagg
     8881 gaatccatcg agattatgtt actattcaat atcaaaattc agaccgtatt tctcttccaa
     8941 ttgaccaaat tagtagctta tcaaagtacg tttctgctga tggaaaagaa cctaaaatta
     9001 ataaactcaa tgatggtcgt tttcaaaaga caaagcaaaa ggtcgctaga caagtagagg
     9061 atattgctga tgaccttcta aaattatatg ctgaaagaag tcagcaaaaa ggattttcat
     9121 tttcaccaga tgatgacttg cagcgcgctt ttgatgatga ttttgctttt gtagaaacag
     9181 aagatcaact taggtctata aaggaaatta aatctgatat ggagagcatg cagcctatgg
     9241 atcgtctttt agtaggcgat gtaggatttg gtaagacaga agtagctatg agggcagcct
     9301 ttaaagcggt gaatgatcac aaacaagtag ctgtcttagt tccaaccaca gtcttggccc
     9361 agcagcatta tgaaaatttc aaagcacgct ttgaaaatta ccctgttgag gttgacgtct
     9421 taagtcgttt ccgtagtaaa aaagagcaag ctgaaacact agaacgtgta cgaaaaggtc
     9481 aaattgacat tattatcgga acccatcgac tcctatcaaa agatgtggtc ttttctgatt
     9541 taggattgat tgtgattgat gaggaacaac ggtttggtgt taagcataaa gaaactttaa
     9601 aggaattaaa aactaaggtt gacgtcttaa ccttaacagc tactccgatt cctagaacct
     9661 tacacatgtc tatgttaggt atccgagatt tatcggttat tgagacccca ccaaccaatc
     9721 gttatcctgt tcaaacctat gttttggaaa ataatccagg tctcgttaga gaagctatca
     9781 ttcgtgaaat ggatcgcgga ggacaaattt tttacgttta caataaagtt gacactattg
     9841 aaaagaaagt tgcagagcta caagaattag tcccagaagc ttctataggt tttgttcatg
     9901 ggcaaatgag tgaaattcaa cttgaaaata ccttgattga ctttataaat ggtgattatg
     9961 atgtccttgt ggctacaaca atcatcgaaa caggagttga catttctaat gtaaacactt
    10021 tgtttattga gaatgctgat catatgggat tgtcaacttt atatcaacta cgaggtcgtg
    10081 taggaagaag taatcgtatt gcctatgctt acctgatgta tcgtcctgat aaggtcctaa
    10141 cagaggtttc tgaaaagcgt ttagaggcta ttaaaggctt tactgaatta ggttcaggct
    10201 tcaagattgc tatgcgagat ttgtctatac gaggagcagg taatatttta ggagcttctc
    10261 aaagtggctt tattgattcg gtcggttttg aaatgtattc tcagttattg gaacaggcta
    10321 ttgctagcaa gcaaggaaaa acgactgttc gccaaaaagg gaatactgaa atcaatcttc
    10381 agattgatgc ctatttacca gatgattata ttgcagatga gcgccaaaaa attgacattt
    10441 acaagcgtat tcgagaaatt caatcaagag aagattatct caatttgcag gatgagctga
    10501 tagatcgttt tggagagtat cctgatcaag ttgcctattt gttagagata gctctgctaa
    10561 aacattatat ggacaatgcc tttgcggaat tagttgaacg caaaaataat caggtcattg
    10621 ttcgatttga agtaacttct ttaacttatt tcttgactca ggattatttc gaagccttat
    10681 cgaaaaccca tcttaaagct aaaattagtg aacatcaggg gaaaatcgat atcgtctttg
    10741 acgttcgcca tcaaaaagat tatagaattt tagaagaatt gatgttattt ggagaaaggc
    10801 ttag
//
LOCUS       DAUCWU010000055        10541 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUCWU010000055 DAUCWU010000000
VERSION     DAUCWU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.055"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            40..573
                     /locus_tag="VGY24_001251"
     CDS             40..573
                     /locus_tag="VGY24_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ4284231.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            840..1526
                     /gene="covR"
                     /locus_tag="VGY24_001252"
                     /gene_synonym="csrR"
     CDS             840..1526
                     /gene="covR"
                     /locus_tag="VGY24_001252"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ4284232.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            1532..3034
                     /gene="covS"
                     /locus_tag="VGY24_001253"
                     /gene_synonym="csrS"
     CDS             1532..3034
                     /gene="covS"
                     /locus_tag="VGY24_001253"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ4284233.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITPRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            3248..3742
                     /gene="nrdR"
                     /locus_tag="VGY24_001254"
     CDS             3248..3742
                     /gene="nrdR"
                     /locus_tag="VGY24_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ4284234.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            3726..4901
                     /locus_tag="VGY24_001255"
     CDS             3726..4901
                     /locus_tag="VGY24_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ4284235.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGNLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            4902..5804
                     /gene="dnaI"
                     /locus_tag="VGY24_001256"
     CDS             4902..5804
                     /gene="dnaI"
                     /locus_tag="VGY24_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ4284236.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLEWKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            5867..7177
                     /gene="der"
                     /locus_tag="VGY24_001257"
     CDS             5867..7177
                     /gene="der"
                     /locus_tag="VGY24_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ4284237.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINLTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            7384..10482
                     /locus_tag="VGY24_001258"
     CDS             7384..10482
                     /locus_tag="VGY24_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ4284238.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASDTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQIDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIKRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMQDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGESGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
BASE COUNT         3399 a         1757 c         2206 g         3179 t
ORIGIN      
        1 agtagtaaaa ataaatgaga gttttattag gagaagatga tgttagctat ttccgaaatc
       61 agaaaacacc cagacggtct ctcgtttgat cgtttatgtg atgtcaaaag tatgttactt
      121 gagcgtgacc aacagattat tgacataaag gcagttaaag ctgtcggtaa tgttcgctat
      181 gataaggggc tttatctgtt agattatcaa ttgagttacg aagtgatatt accgtctagc
      241 cgttctatgg ttccggtctg tttaagtgaa gtccaacaca ttcaagaatt atttatagag
      301 gcgacagatt tagctgataa gaaagagttg gttgaagaca atctggtatt agttttagac
      361 aaagacgcta ttaacctcga agaaagtatt gtggataata tcttacttgc tattccgcta
      421 caggtcttga cagaggaaga aaagaaatca aaagagttgc cagcaggtca aaattgggca
      481 gttttgacag aagaagacta ccagtgtcta aaagaagaaa agcaaaaaga aaataatcct
      541 tttgctagct tgcaagggtt gtttgatgaa taaaactgaa tattaaagat gtctgtaatt
      601 tttatttgaa aaaacagcta ttctttgata tactctttag agaatatggt tacttatcta
      661 aagtagaagg tattttgacc atagagggca aagaaggtaa tgactattag atgcttcata
      721 ttttattcgc tagaagacta tttgaccatt agaactgaaa tagtctagga tatgagatgg
      781 atttcatagt tttctttctg ggagaaaaag atagattaag aggataaggg ttggtataaa
      841 tgacaaagaa aattttaatt attgaagatg aaaagaatct ggctagattc gtttctcttg
      901 agctgcaaca tgagggttat gaagtcattg ttgaggtcaa tggtcgcgaa gggttagaaa
      961 ctgctttgga aaaagagttt gatttaatcc tgcttgactt aatgttacca gagatggatg
     1021 gttttgaagt gacccgtcgt ttgcaaaccg aaaaaacaac gtatatcatg atgatgactg
     1081 cgcgtgattc tattatggat gtggttgcag gtttagaccg cggtgcagac gactatattg
     1141 ttaaaccgtt tgccattgaa gaactacttg cccgtattcg tgctattttc cgccgtcaag
     1201 atattgaatc tgagaagaaa gtgcctagtc aaggcattta tcgagatcta gttttaaatc
     1261 cacaaaaccg ttcagttaat cgtggcgacg atgagatttc tctcactaaa cgtgaatatg
     1321 atttgcttaa tattttgatg actaacatga atcgtgtcat gacacgtgaa gaattattgt
     1381 caaatgtttg gaaatatgat gaagccgttg agactaatgt tgtagatgtc tatattcgtt
     1441 atctccgcgg caaaattgac attccaggca aggaatctta tatccaaaca gtgcgtggca
     1501 tgggatacgt tattcgtgag aaataagtca tatggaaaat cagaaacaaa aacagaagaa
     1561 atataaaaac tcgttaccaa aacgactatc taatatcttt tttgttcttt ttttctgcat
     1621 tttctctgcc tttacactga ttgcctatag ttcaacaaac tatttcttat tgaagaagga
     1681 aaagcagtca gtctttcaag ctgtaaatat tgttagagtt cgtctttctg aggtggactc
     1741 taattttaca ttagagaact tagcagaagt tttgtacaaa aacgataaaa cacatctgag
     1801 aattgatgac agaaagggca gtcgagtcat taggagtgag cgcgatatca caaatactct
     1861 agatgcgaat caagatattt atgtctataa cattgataaa cagatgattt ttaccacaga
     1921 caacgaagaa tcatctcctg gcttgcatgg tcctatcggt cgggtgtatc acgaccatat
     1981 cgaagatcag tatcgtgggt tttccatgac acaaaaggta tattctaatc ggactggaaa
     2041 atttgtgggc tatgttcaag tctttcatga tttaggcaat tattatgtca ttagagcaag
     2101 actgctgttt tggctactag ttgttgagtt atttggcaca agcttagcct atttaatcat
     2161 cttaattact ccgcggcgct ttttgaagcc tttacacaat ttacatgaag tgatgcgtaa
     2221 tatctctgaa aatcctaata atttaaacct gcgctcagat atttcgtcag gagatgaaat
     2281 cgaagaattg tctgttatct ttgacaatat gttagacaaa ttggagacac atactaagtt
     2341 gcaatcacgt tttattagtg atgtcagcca tgaattacga acaccggttg cgattattaa
     2401 agggcatatt ggtctcttac aacgttgggg taaagatgac agtgatattc ttgaagaaag
     2461 cttgacagca acagcgcatg aggctgaccg tatggcaatc atgattaatg atatgttaga
     2521 catgattcgc gtacaaggct catttgaggg acatcaaaat gatatgacag ttttggaaga
     2581 ttctattgaa actgttgttg gtaattttag agttttaaga gaagatttta tctttacatg
     2641 gcagtcagaa aatccaaaaa cgatagcccg tatttataaa aatcattttg agcaggcttt
     2701 gatgattctg attgacaatg ctgtgaagta ttcccgtaaa gaaaagaaaa tcgcgattaa
     2761 cctttcagtg actggcaaac aagaagctat tgttagagtt caagataaag gcgaaggaat
     2821 ttctaaagaa gatattgaac atatctttga acgcttttat agaacggata aatcacgtaa
     2881 tcgaacaagt acccaggctg gattagggat tggcttgtct attctcaagc aaattgtaga
     2941 tgggtatcat ttacagatga aggttgaaag tgaattaaat gaaggttcag tgtttatctt
     3001 acatattcct ttggcccagt ctaaagagag ttagagtagc ttaaggcatt gttaatgtga
     3061 caggtatagt atgtaatcat tagataatag tagcagtctt ttgccacaac tggttagaag
     3121 ctgatgctat tttttagcaa tgatggatga catgaaagaa atactgtcaa tcgcgatgcc
     3181 ttttgttatc cttgagaata tggtaaaata gtgtattaga ttaatttgat agaattgggg
     3241 tgtttgcgtg cgttgtccaa aatgtaatta ccataaatcg agtgttgttg acagtagaca
     3301 agctgaagac ggcaacacca ttcgtcgtcg aagagaatgt gaacagtgtc atactcgctt
     3361 tactaccttt gagcgtgttg aagaactccc tttattagtc atcaaaaaag atggcacacg
     3421 agagcaattt tctagagata aaatattaaa tggggttgtc caaagtgcgc aaaaacgacc
     3481 tgtctcgagc acagatattg aaaatgtcat ttctcgtatt gaacaagaag ttcgaacaac
     3541 ctacgaaaat gaagtgtcaa gtactgctat tggtaattta gtgatggatg agttggccga
     3601 acttgatgaa atcacatatg tacgctttgc tagtgtctac aagagcttca aggacgttga
     3661 tgaaatcgaa gaacttttgc aacaaattac gaatcgcgta cgcggaaaga aaaaacgttt
     3721 aaataatgat gaaaccaatt gatactttta catatctaaa acgcaacaaa gtaactcttg
     3781 attcggtgac tttaatacag ttgtactttc caattattgg aagtgacgca gtctctatct
     3841 atcaatactt tattcacttt tttgacgatg gtttacaacg tcataaattc agcgatattt
     3901 taaatcacct acagtttggt atgaaacgtt ttgaagatgc tcttgccata ttgacagcaa
     3961 tggaattagt gagtgtgtat cagctatcag atacatatct cattacgttg caccagccgc
     4021 ttagccgtga cttgtttttt caacatccag cttattctcg attattagaa caaaagattg
     4081 gagaggtagc tgtctctgag ttgcaggtaa ctgtcccaag tcaagcaaga aatatttcta
     4141 agcgattttc ggatattttt ggggtgcaag gaaatttgac gaacgtacct caaaagccac
     4201 aaaagaattt tgacctttca agtttccagc aattaatggt tcgtgatggg ttacaatttg
     4261 aagataatca aaaagatatc attagccttt acagtattgc tgaacagtat gatatgacgt
     4321 ggtttgatac ttatcaaata gctaaggcga cggctgttaa tggaaaaatt cgacctgaac
     4381 gcttattggc taaaaaaaat caaagcatga caaaaccaag caaagaaaat ttttcacagg
     4441 ctgagcaaat tattttgcga gaagcaaagc aagacagtgc tttagtcttt ttggaaaaaa
     4501 ttaaaaaggc tagacgtgca acgattacta aggatgaacg cattttattg caaacattgg
     4561 ccaaaatgaa ttttcttgat gatgttatca atgtcatggt gctttatacc tttaataaga
     4621 caaaatctgc taatttacag aagagttatg tcttaaaaat ggccaatgat ttcgcctacc
     4681 aaaaggtgtc aacggctgaa gaagctatag tggttttgcg agccttcacg gatcgtcaaa
     4741 gtcgtcggca atcaaaagta aaaacaagtc aaagtaatgt gcctaagtgg agcaatcctg
     4801 attaccaaga gacaacaagt caagaagagc aggcgaaact agatcagttc aaacaggctg
     4861 ctttgaagag gttagaaaac ctaggaaaag ggggtgacta gatggaaaag attggagaaa
     4921 ccatggcgaa attgggtcaa aacactcgtg ttaatagcga ccagttgatt caaaccattt
     4981 tagcggatcc tgaagtagca agcttcatca gtcaacatca cttatctcaa gagcaaatta
     5041 accttagctt atctaagttc aatcaatttt tggtagaacg ccagaagtac caactcaaag
     5101 atccctctta tattgctaag ggctatcagc ctattctagc tatgaatgag ggctacgctg
     5161 atgtgtctta tttggaaact aaagaattgg tagaagctca aaaacaggcc gctatctctg
     5221 aacggattca actggttagt ttgccaaaat cctatcgcca tattcattta tcagatattg
     5281 atgttaataa tgcgagccgt atggaagcct tctctgctat tttagatttt gtggaacaat
     5341 acccatcagc agagcaaaaa ggcttgtatt tgtacggaga tatgggaatt ggcaagtctt
     5401 acttgttggc tgctatggcc catgaattgt ctgagaaaaa aggtgtgtca acgacccttc
     5461 tacattttcc gagctttgcc attgatgtaa aaaatgccat ttcaaacggc tctgttaaag
     5521 aagaaatcga tgcggtcaaa aatgtaccag tgctcatttt agatgatatt ggtgcagagc
     5581 aagccacttc ttgggtccgt gatgaggtgt tgcaggtgat tttacaatac cgtatgttag
     5641 aggaattgcc aaccttcttt acctctaatt acagctttgc ggatttggag tggaaatggg
     5701 caaccatcaa aggaagtgac gaaacttggc aggccaaacg tgtcatggaa cgcgtgcgtt
     5761 atttggctag agagtttcat ttagaagggg ctaaccgccg ttaagtttga gatagcaatc
     5821 aagaagaata gctacacgta tgagtgagtg aaagaaaagg agaatcatgg ttttacctac
     5881 cgttgccatt gtgggccgtc caaatgtggg caagtccacc ttatttaatc gaatcgcagg
     5941 agagcgcatt tcaattgtag aagacgttga aggggttact cgtgaccgta tttatgctac
     6001 tggggagtgg cttaaccgcc aattttcatt gattgataca ggtggtattg atgacgttga
     6061 tgccccgttt atggagcaaa tcaagcacca ggctcaaatt gccatggaag aagctgatgt
     6121 tatcgttttt gttgtttctg gcaaagaagg ggttacggat gcagatgaat atgtgtctaa
     6181 aatcctatac cgaaccaata ctccagtgat tttagctgtc aacaaagtgg ataatcctga
     6241 aatgcgtaat gacatttatg atttttattc acttggttta ggggatccct atccagtttc
     6301 ttctgttcat ggtatcggaa caggggatgt cttggatgcg atcgttgaaa acttgccagt
     6361 agaagaggct gaagaaaatg atgatattat tcgcttcagt ttaatcggtc gtcccaatgt
     6421 tgggaaatct agcttgatta acgctatttt aggtgaggac cgtgttatcg ctagccctgt
     6481 cgcagggaca acacgtgacg ctattgacac gcattttacg gatgctgacg gtcaagaatt
     6541 taccatgatt gacacggctg gaatgcgcaa atctggcaag atttacgaaa atacagagaa
     6601 atattctgtc atgcgtgcca tgcgtgccat tgaccgctcc gatgtggtct tgatggttat
     6661 caatgctgaa gaaggtatcc gtgaatacga caagcgtatc gctggctttg cacacgaagc
     6721 tggtaaagga atgattatcg tggttaacaa atgggatacc attgacaagg ataatcatac
     6781 cgttgctaag tgggaagccg atattcgtga ccaattccaa tttttgacat acgctccgat
     6841 tatttttgtg tcagctttga cgaagcagcg cctcaacaaa ttgcctgact tgattaaacg
     6901 tatcagtgaa agtcagaata aacgtatccc atctgctgtc ctcaatgatg ttatcatgga
     6961 tgctattgcc attaatctaa cgccaacgga taagggtaaa cgtttgaaaa ttttctatgc
     7021 cactcaagtg tcagtaaagc caccaacatt tgtcgtcttt gttaacgaag aagaattgat
     7081 gcacttctct tatctacgct tcttggaaaa ccagattcgc gcagccttta cctttgaagg
     7141 gacaccgatt catctgattg ctcgcaaacg gaaataagaa atgaacatca actaaaatgc
     7201 tgacctaaga aataatctca tcattgataa ggactgtgtt gtgtatgtta caggagacag
     7261 tcctttttat aataccttta agggtgtaga cggaagatta gtagcaaaca aagaaaaagg
     7321 attttgacaa gaaatatcag tcggtttttg ctataatgaa aggataagca aaaaggtgga
     7381 aaaatgatgg ctagattaat tccaggacga gtaaggaatg aaggtattaa gctttatgag
     7441 caaggtttag tctcattcca agatgataat aaaggcattc ttcaaataga agtggagact
     7501 tatcaggttc aatatggagc agatgatgag gatatcactt gtcaatgcga cacatttcat
     7561 atgaaacatt attgcaagca tattgcagca gtcgagtatt ttttaaaaaa tgatcaaaaa
     7621 ggtaagcttt ttttaaaaca gttaacaaat caaacaaaaa tcaaagaaac aacaaagaaa
     7681 atgacatctt ttggtagcct ctttttagat agcttagcca tgaatgaaga tgattctgtt
     7741 aaatacaggc tgtctgctct tggtagtcga agcccttttt cgtcagatta ctggtggagt
     7801 ctgaaaatca atcgtttacc agatgatcgt tcttatgtga ttcgagatat taaaggcttt
     7861 ttgcagctta ttaaaaaaga aggcttttat cagattggaa aaaattattt tgaacaactt
     7921 tcatggctgc agtttgatcc atccagtcaa gaccttattg agtttttatg gcgcttagca
     7981 tctgatactg acaaagggga caatgagaac atttttccaa atcatgcaag gcatttgcgc
     8041 ttacctagtg gtttttttga agaaggcatt cactatttga cgtcacttta tgactttact
     8101 tttgagggac cttctcaaac ttatcatcat ctttttgtcc gttctttgga agcagaggca
     8161 ggcctttatg agtttaaagt ggaagtgcat cgcaaatcta ttgaattgca aattgctgaa
     8221 aaaaatgtgc aatacctctt tgataatgac tatcttcttt atcaagatac cttttatcac
     8281 ttgacgttaa agcaacgaaa aatggttcag gctattcgta gtctgcccat tgaggccgac
     8341 ttggctaaac atattcattt tgatttggat gatcatgcga aattagctgc aagcttatca
     8401 gattttaaac agatcggtct agtagaggct cctaaaagct ttgctattag agactttgag
     8461 gtgacttttc aaattgattt gttaaatcga gatgaaatca gttgtcagct tatgtttgat
     8521 tatggcaatt accaagttag tgacaaagca agtttagaag cacttccttt tgctagtcat
     8581 ttaaaaaaag aagaaaaaat caatcgttct ttacttgctt ttggcttctc gccacaattt
     8641 tactcaaaaa agcgactaac aagtgcaaaa gagctctata ctttttttga agaaacagtt
     8701 ccatgttttg agcgcttagg taatgttgcg ttgagtacag ctatccaagc attacaagtg
     8761 aaagaaatgc ccaagatcgc tattaaacgt aatcaggggt tactagacat ctcgtttgat
     8821 ttttcaacca ttattgaaaa tgatattgac caagctgtta cagctctttt tcaaaataat
     8881 ccttattttg tgagtcaaac aggtcaatta gtggtttttg atgatgaaac tcaaaaagtt
     8941 agcaaaagtt tacaagaatt aagagcaaga cagctaaaaa atggtcactt gcaactggat
     9001 ggcattagag ctctacaagt atccaaactt tttgaaggca tgacatctgt tcatttttca
     9061 aaggaattag aagagttagc ctatcatttg cagcatcctg aaactttttc aataaaaccg
     9121 ttaccagtta aggctcaaat gcgagattat caacgcaacg gtgttcagtg gttatcaatg
     9181 ctcaatcatt atggctttgg tggcatttta gcagatgaca tgggacttgg aaaaactctc
     9241 cagacacttg cttttttagc tagtcacctg aaatcagata gtaaagtgct cattttatct
     9301 ccatctagtc ttatctataa ttggtttgat gaatgtcaaa aattcacacc tcagctagat
     9361 gttgtggttt cttacggact taaacaaatc agagatcaaa tcatcgaaga aggtcatcaa
     9421 ataaccataa ccagctattc ctcctttaga caggattttg agacctatca agcgtttcac
     9481 tacgattatc ttatcttaga tgaagctcaa gtgataaaaa atgcacaaac taagatttca
     9541 cactgtttac gtgcgtttaa tacagccaat tgttttgcgc tttcgggcac tcctattgaa
     9601 aataaaatgc ttgaaatttg gtccattttc caaattgttt tgccagggtt gctaccgact
     9661 aaaaaagagt ttttaaaatt aacagctgag caagtatcac gctatatcaa accctttgtg
     9721 atgcgtcgta aaaaagaaga tgtcctacca gaattacctg atttaattga aactaattat
     9781 tcaaacgaga tgacagatga acaaaaagct atttacttag cgcagttaag gcagatgcaa
     9841 gatcaaattc gaaattctag tgatgtggat attagccgtc aaaaaattga gattttatct
     9901 ggtatcactc gattgaggca aatctgtgac actccaagtc tctttatgga ttaccaaggc
     9961 gaaagtggta agctagatag tttgcgaatc ttattgacac aaattaaaga aaatggtcat
    10021 cgggctttga ttttttctca atttagaggg atgctggact tggccaagca agagatgact
    10081 gctctaggat tgacctctta tcaaatgacg ggatctaccc ctgccaatga acgtcaagaa
    10141 atgactagag cctttaataa cggttccaag gatgcttttt tgatttccct caaggcaggt
    10201 ggagttggaa tcaaccttac gggagcagac acagtgattt taatcgattt atggtggaat
    10261 cctgctgttg aaatgcaggc aattagtcgc gcctaccgca ttggtcaaaa agaaaacgtc
    10321 gaagtatatc gcttaatcac tcgaggaacg attgaagaaa agattttaga gcttcaagaa
    10381 agtaagcgca atttggtgac tacagttttg gatggcaatg aaagccgagc aagtatgtcc
    10441 attgaagaaa taaaagaaat tttgggacta aacactcatt aatcttcctt gattttagtg
    10501 acattaaaaa ccctttaaag aagatgacac caaaagcacc t
//
LOCUS       DAUCWU010000056        10256 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUCWU010000056 DAUCWU010000000
VERSION     DAUCWU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.056"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..737)
                     /locus_tag="VGY24_001259"
     CDS             complement(<1..737)
                     /locus_tag="VGY24_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ4284239.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFARLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYL"
     gene            complement(737..1666)
                     /locus_tag="VGY24_001260"
     CDS             complement(737..1666)
                     /locus_tag="VGY24_001260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ4284240.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(1681..2316)
                     /locus_tag="VGY24_001261"
     CDS             complement(1681..2316)
                     /locus_tag="VGY24_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ4284241.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(2309..3547)
                     /gene="hflX"
                     /locus_tag="VGY24_001262"
     CDS             complement(2309..3547)
                     /gene="hflX"
                     /locus_tag="VGY24_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ4284242.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDVAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(3620..4519)
                     /gene="miaA"
                     /locus_tag="VGY24_001263"
     CDS             complement(3620..4519)
                     /gene="miaA"
                     /locus_tag="VGY24_001263"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ4284243.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVNRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            4653..4829
                     /locus_tag="VGY24_001264"
     CDS             4653..4829
                     /locus_tag="VGY24_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ4284244.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(4947..5855)
                     /locus_tag="VGY24_001265"
     CDS             complement(4947..5855)
                     /locus_tag="VGY24_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ4284245.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFNYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     VSSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQSK"
     gene            complement(6092..6511)
                     /locus_tag="VGY24_001266"
     CDS             complement(6092..6511)
                     /locus_tag="VGY24_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ4284246.1"
                     /translation="MPFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMI
                     NPHRYTISQDNQALEKVKPASYKRMDIAMTHLDSLIGESGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLTAFMAKMVEDSAVQQVLNAAH"
     gene            complement(6822..7808)
                     /locus_tag="VGY24_001267"
     CDS             complement(6822..7808)
                     /locus_tag="VGY24_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ4284247.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAVKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVVSKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            8009..8311
                     /locus_tag="VGY24_001268"
     CDS             8009..8311
                     /locus_tag="VGY24_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284248.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWREDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(8674..8745)
                     /locus_tag="VGY24_001269"
     tRNA            complement(8674..8745)
                     /locus_tag="VGY24_001269"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8710..8712),aa:Arg,seq:ccg)
     gene            complement(8858..10063)
                     /gene="rpsA"
                     /locus_tag="VGY24_001270"
     CDS             complement(8858..10063)
                     /gene="rpsA"
                     /locus_tag="VGY24_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ4284249.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
BASE COUNT         3120 a         2196 c         1836 g         3104 t
ORIGIN      
        1 agataatcag ataatttagg gaaaagggtg taaaattgat gggtcaccgc taaactaaag
       61 ggcaaattca attctcgttt atttttcccg ataatagaaa ccaaacgctt agccacttga
      121 tttggttgga gagtaaattt accaacgctt tccaaataat gtccagacgg gtcagcttgg
      181 tcaaaaaact tggtggcaat gggacctgga ttcacggtgg tcacataaac ccctttatcc
      241 gctaattcta agcgaagggc attggaaaat ccgataaggg caaacttggt ggctgaataa
      301 atgctcgatt tggctgacgc aatcaagcct gccatggaca caatgttaat aagatgacct
      361 tgcccctgct ctgccatttt ctgaccaatc aagcgagcaa agtgaatgct cgctagggta
      421 ttaacctgaa acatatcagc tatttcttgg gcagaaaact cttcaaagcc tttgaaagct
      481 ccatagccag cattattaat caagacatca atacggccat agcgctggta aatctgggcg
      541 accattttct caatggctac tggattggta atatccaact caaggcattc tttgttgtca
      601 atatgctgat aacagtgttc taggcgttct ttgttacgtc ctaataaaat caagctatct
      661 tccttgggta actgcttaac aattgcctga gccagtcctc cagaagctcc cgtgataaca
      721 atgattcttt gtgccattaa ataatcactt cctctaaatc ttgtaccatt ttaacatttt
      781 caaatatagt ggcggcatct ttttccatct ggcgacaatc acgccctaaa aagcgagctg
      841 atacatgatt gagcaagagg cgttttgcgc ctgcttcatg agcgatttgt gctgcctgca
      901 tgttggtaga atgcccgtga tttctcgcaa tccgctcgtc acccttacca taggtggatt
      961 catgaaccaa aacatctgca tctttagcga gtttaacact ggcagacgtt ttgcgcgtat
     1021 cgccgataat ggtgatgatt ttgccttttt taggggcaga aatataatct ttggcacaaa
     1081 tcaggcggcc gtcttccaac tcaacgtctt gaccattttt aattttgcca aagagtggtc
     1141 caaagggaac acccgctgct tttaaggctt ctgcgtctag ggttccttct aagtcttttt
     1201 gaactactcg ataacccatg caaaaaatgg tatgcgccag acgctctgca tagaccacaa
     1261 acttatctgt ttccattatt ttacctaatg atttgtcatc aaattcatga aaatgaatct
     1321 gataaggtac acgcgctcca gaaactttta gactagttaa cacgtaagtt ttaatgccaa
     1381 ttggtccata aatatcaaga tctgtctgtt cttcactagc ttgaaaagac cgacttgata
     1441 aaaatcctgg taatccaaaa atgtggtccc catgcaaatg agtaatgaaa atctttctta
     1501 tttttcgtgg cttaatcgtc gtttctaaaa tttgtctttg cgttccttca ccgcaatcaa
     1561 acatccagac ttcattgatt tcgtctagta atttcaaggc aagacttgac acattgcgct
     1621 gcttggctgg ttgaccagct ccagttccta aaaattgtaa ttccattcaa tctattcttt
     1681 ctattgggtg atgtaaatca taaattctcg ttgggcaaaa tcataatact tttgaccaga
     1741 aaatgtttgg ctaagagccc ataactcctc tacattaaac cccttaagca tggtgatacc
     1801 gtttgccaga cggtaaatct tcgacaagag tgtgtcatca ggcgtaatgt cttcacattc
     1861 tccttgctca aactgcttag cctgcatttt aagctggcct ttgtcttcat aaaccatata
     1921 ttgtgggaaa atttgtgtta attcaaagaa gagttcgggt ttcttaggct ctgctttttc
     1981 agaaaatacc aatgcaactt cttggtcatt ttggtaagca aaaccataag cttttccaga
     2041 caaattgagg cgtacaaagg gcttttcttc ctcaaaagat ggttggtcag taaaaagccc
     2101 caaagcctta gaaagattaa aataataatc agtcgcagct tgcggtgact gtttctggta
     2161 gatttcagca aagtaggcat taataacact tggtggtggt gtgataaaag ctagtttttg
     2221 ctggtctgtc aaactggcta acagatggtt taggtagttg gtatccagac tagtaaatcc
     2281 accataggtt tttgctaaat caatataatt attcataaaa atcgtcaagt ctccattgct
     2341 gttttgggga aatgtagccc gaaatatcct ctatttcttg gtcaaacgtg tagtggtcta
     2401 aaagagcaac tcggttcaga tcatacagct tgtacgcctt gtcttgatgc actttgattc
     2461 taaaaggaac aaactggtct cgaatttggt caatgattaa acgtctcaag agaattttgc
     2521 tatcctttga acgggctgaa atcctgatat ttggaaaagc agtagctgtg aattgttctg
     2581 ctacatccac cttgttataa atagctaaac gtggaatatt aagcatgtct agctctttta
     2641 gaaggtttaa aaccactttt tcttgctcac tatgattagg gtcgctagcg tcaatcacat
     2701 gtaacagcaa atccacatat ttgctttctt ctagggttga tttaaaagca gccactaatt
     2761 ccgttggcaa gtcttgaata aagcctacgg tgtctgttaa ggttgcttga aattgatttt
     2821 ctaaatagag ttgctttgta gttgcatcta aggtcgcaaa taattcattg gcttcatagt
     2881 ggctgtcatc tgtcaacaag ttcataatgg ttgacttgcc agcattggtg tagccaatca
     2941 agccaatttt aaaggtatct gaacccacac gacgatctct aattgtttga cgatttttct
     3001 ctacctgagt taattggcgt tcaatatcag caatctgatg acgaatcgaa cggcgattta
     3061 actctagttg gctttcacct ggtccacggc tcccaatacc acctgcttga cgacttaaca
     3121 tgatgccctg tccaaccaaa cgaggtaaca tatatttcaa ttgggcaagg tgtacttgta
     3181 atttgccctc atggcttctg gcacgcatgg caaaaatatc taagattaac tgcatacggt
     3241 caatgacttt tacctctaaa acagcttcta gattagcatt ttgcctagca gtcaaccgat
     3301 tgttcacaat aactgcatca atctcatcag cttccacaat cgccttaatc tcatccagtt
     3361 tccctgagcc gataaaggtt ttgctgtcgt aacgttctcg tttttggctg aaacttgcca
     3421 taactttgac gccagctgtt ttggcaagat tagctaattc tgtcatggac atgtcaaaat
     3481 gttctgttgt ctgaagctca actcctaaaa gaatcactct ttcttgctga cgctttgtct
     3541 caatcatcac ctttaccaat cctccaaatt ccgtaaattc tattataaca tatcttcaac
     3601 agattctgct aattttgcgt catgacttct ctttctgacc aagaaaatcc ctgaccctat
     3661 catgtactac ctgtgggtaa tcaggagccg ttatggcagt gaagctaaca gccattcgat
     3721 tgcggaacca agttagctgt cgcttggcaa aacgtctcgt gttttgtttt aactgatcac
     3781 tagcttctgc caaagtcatt tcacctacaa aataggggaa taattcctta taacctatgc
     3841 cacgactagc ctgaacagtc gggtaatgct catataacca ttttgcttct tccaataagc
     3901 cgttttcaag catacgattc acgcgctgat tgatacggtc atagatgact tggcggtcat
     3961 cattcaagcc gataataagc ggctcataag ccgtctcggc attctctaat tcatcagcaa
     4021 actgagccaa ttcaagggct cggatcgccc gcctgcgatt cacctgctca atggtaatgt
     4081 tattgacttg caagcgttca tacaagtcgt gatcgtccaa ttgctccaac tcattccgat
     4141 aggcttttac cgcctcttga tcaacttgac ctcctagatg ataaccttcg agtaaacttt
     4201 gtaagtaaag accagttcca cctacaataa ttggtaattt cccacgacta acaatatctg
     4261 aaatagcttt ttgagcatct tgaacaaaat cgtaggccga ataagactcc gtcacctcac
     4321 gaatatcaat taagtgatga accgctgctt cttgctcttc ttgagtggcc ttggctgttc
     4381 caatatccaa ctgtcgataa acttgctggc tatctcctga aataatttct ccattaaaag
     4441 ccttggctaa actaatccca agtgctgttt ttccaaccgc tgtcggacca acaatcacaa
     4501 ctatttttat ttttgtcatt ttcttgtctt tctattagaa aagcgtgtcc aaaaacgcct
     4561 tttacttgaa aattagctta tttttcgcta ccatatatta taacataaga ttaaggacca
     4621 ctgaggtcct tcactagaaa tggagaataa tcatggttaa gaaatatcaa tttgttaaag
     4681 gtcttgctac tggcgtccta gcaactgctg ctactgttgc tggtgctgtc tttgctgtaa
     4741 aaaaaacaat cattgaccct gaagaagaaa aagctgcttt cattgaagaa aaccgcaaaa
     4801 aagctgcgcg tcgtcgtgta gctcgctaac gtgacaatgg atcaatttaa aagagactag
     4861 cacattaaag tgccggcctc ttttttagta atctcgataa ctagttagta gtaactgtct
     4921 cttgatgagc caagatgtta gctgctttac ttactttgaa atagaaatct caggtaagcc
     4981 ccataaacat aagtgactaa agctgtcgcc aacaatacct ctcctagcaa aagaacctgc
     5041 caacctaaac cttgccaacc taaaaaagat aaggtcattt ttaaggaagt ggcactaatc
     5101 acaaacggaa aggtaaaagc tgaaaaacct gggttaaagg gacgattgag taagcgagga
     5161 agctgtacaa ccacaaaagc atataacagt tgtgagctga cgagaagaaa gataaccatt
     5221 cctacttgtg gtctaggaaa ggttgctaaa taagacgctg aaagtaagga cagaggggca
     5281 caaaacgttg aaatattggg cattactgct tgacctagac caatcccata agtctttttg
     5341 gccataaaag gtaaaatcac agcagttagg agaagacaaa cccaaaaaat cacctgtcct
     5401 aataaaaact gcctactagc aggtgctgtt aatgccgcca ttgcaattcc aacaaaaagc
     5461 acggaccagg aaggaaaaac gttatcccat gaaaaagaaa agacaaaacg atactggtaa
     5521 taagcaatca ggacaagatt tcccaaaaag aatagccacc aagtgagaaa gcctaaccaa
     5581 caccctcctg tcactttgag aaagagtgaa gacaacaaca tccctgtcat aaaaaaagtt
     5641 ggaaaaacag aggcaattag aggttgtcgt aactgcattt tggtatcgtt aaggttccta
     5701 accatgccaa caagcaagat accatagata aaaagagctg ccagaattcc caaataatta
     5761 aaaatcgaaa cataagtagc caacaaattg ccaaatgaca acgttcctaa tgccaatcct
     5821 gacatgacca atggtgggtt tttaaggtgt ttcataagtg tccttttctg cctattaaca
     5881 gctattttta gtgacataat cttgtttatt ttatcacatt tcgagcattt ttgacaccac
     5941 ccgcgaaaga aaatttaagg ctcttgccat tgttttttta cattctcgca aaactaggaa
     6001 actgctcaaa aagctaagga gtgaccactc ttctctagaa aaatgaaaga gtagagaaga
     6061 cttggtctga tctttacttc ttctttttga tttagtgagc tgcattaagc acttgttgaa
     6121 cagctgaatc ttccaccatt tttgccataa aagctgtcag atgcggataa ttctcgtaag
     6181 attttggtgt tttttgagac catagcgcca tggcgtaagc ataagcatct gcaatggttt
     6241 gttgatctcg ataaacatgc ccactctccc caataagact atctaaatgc gtcatagcaa
     6301 tatccatccg cttgtaagaa gctggtttga ctttttcaag cgcttgattg tcttgagaaa
     6361 tagtgtagcg atgagggttg atcattggcc taaaaacaag gggaaaatca cctgttaaga
     6421 agtccatgat ttcgttgaac cgactttctg ccaaaagatt atcttgggga atgagctgag
     6481 atttcagggt ttgtttcgct ataaagggca agataatacc tgcctgtgtg acaatttgac
     6541 catcatcaag ctgtaaagct taaaccattc ccaaaggatt gatggtaagg tagtcttcag
     6601 aatccagttt taccttctct atctcgtaat acagaaactc acaattcttt tgaaaaggtt
     6661 ctagacagag ttggtgttga cgtcttcgct gttgatggct ctgcatgaca aaatgagctt
     6721 ggtgtccacg ttcactggta tcttgttgca aagtctttgc aatttggtct catcatctgt
     6781 atcacaaaaa acaaaccagt ttcctgattt gttttgagat attaggcacc tacaaattct
     6841 ggcaaggatt ctccaatagc ttctaaacgt tctgcccatt cttgacgtga taaaccattt
     6901 tcttgcacat agcgattacg ttcatgtgca cgacattcag gcgagcatcc tcgaacatac
     6961 tttgtttcgt tttcttctga agcaaaaatt tgtttgttac aaaacggatt tgcacaattg
     7021 acataacgtt cacaaggtgt tccatcaaaa taatctttac taacgacagt tggattaaca
     7081 tgattaattg gcaccgagat acgatcatca aaaacataca tcgcaccatc ccaaagttca
     7141 ccttgaactt ccggatcttt tccatatgtt gcaataccac cgtgtaattg tccaacatct
     7201 ttaaagcctt ctcgaaccat ccaacccgag aatttctcac agcgaacacc accggtacag
     7261 tacacgacaa cacgtttttc cataaactta tctttgttgt cacgtaccca ttgtggtaac
     7321 tcacggaaat tgcgaatatc aggacgaatt gctccacgga agtgacctaa gtcatactca
     7381 taatcattac gcgtatctaa aacaacagta tcttcatcaa gcaaggcttc tttaaattgt
     7441 ttaggattta aatactcacc tgttgtttcc aaaggattaa tatcactatc aaagttattg
     7501 tcttctaacc caaggtgcac gatctctttc ttataacgaa caaacatttt acggaaagct
     7561 tgttgatttt cctcgtcaat cttgaaccaa agatcagcaa aacgctcatc gctgtggacc
     7621 caatccatgt acttctgagt tgtttcataa tctccagaaa cagttccatt aatcccctca
     7681 tccgcaatca aaatacgacc tttaagccca atagatttac aaaattctag atgtttcaca
     7741 gcgtactctt gcgcattttc aattgaaaca tatttataat agagtaaaac tctgattttt
     7801 tctgacattt tttctccttc attgttattc agttacttcc tattatatct aggaaaaaca
     7861 agtctaggca agccatttgt ttgtcttttt tttcacaaaa tagatctcaa tttatttttg
     7921 atgagatgag atggcaaccc attactcctt taagcaaatc attagaagct ttatttagaa
     7981 aatgctaaga taaaacaagg aggccaaaat gacttatgaa ctttgtttag aatatgggac
     8041 ttatccgttg tcacgtgttg atgcttactg gagagaagat caaaatcccc caacattcat
     8101 tcaagaagat cgactgcttt gccataaact agaaactatg aatcacctgt tccatgattt
     8161 atttgtaact attgaatcac aattccatta tgttggtttt aacatgcctg aaaaaagagc
     8221 gcagattcgg atactatatc aagaagtagc tactatatta aaatccaaat ataaggatta
     8281 tccaattaag attgagacgt ttcttcttta aaaatctaat actaaaaaac taactgtgct
     8341 tagatagatg acacagctag ttttttaggt aaaactcttc ttaaatttta aaacgtttcg
     8401 aaaaatcact aaatgcttta atagcacttg ctaaaaatgg tttcaccatc tcatcattaa
     8461 ttaaccttga ttacttacaa tcttatgaat ctgagtcaaa taaaattcag gctgtggcat
     8521 tacagagaca tttaaaacct atactcatcg ttttcaaggt cagaaagaga atgattttga
     8581 aaaggttcgt caactcttct agataatttt ggacaaattt tgatcaagaa acaaaaagag
     8641 cctatcaaat aaggcttttt tatatgattt ctatccacct tgagggaatc gaacccccat
     8701 ctcaagaacc ggaatcttac gtgatatcca ttacactaaa ggtggtcaca actttgtcaa
     8761 tacccttata gtatatcata aaatgaaata atatgaaata acttttctaa ccagaagaca
     8821 ataaaaagtt gaggaaaacc ttaacttttt agatttatta caattcaata tcaccgaaaa
     8881 gatcagccat tgagaatcca gtttgtgttt ctgggagttc gtagtcacgt cttgattcac
     8941 gttttggacg acgtgggcgt gattgacgtt tttcttcttt gttgtctcct tcagcttgcg
     9001 ctgggcgttc ttcaagagct ttgattgata atgatacgcg ctcatcagct gcattcactt
     9061 caagaacttt aactgttact tcttgtccta cagaaagtac atcttttgga ttttcaacac
     9121 gtttgtgtga aatttgtgaa atatgaacaa gtccatcaat acctggtaat acttcaacaa
     9181 aagcaccgaa gtcagtcaag cgttttactt taccttcaac aacatcacct tgagcaagtt
     9241 tttgttcaac gccgtcccat ggtccaggtg ttgttgcttt aagtgaaagt gacacacgac
     9301 cagcttcttc gtcaattgaa agaactttaa cttcaacttc ttcaccaact gaaacaactg
     9361 atttaggtga cacgttacgt tcgtgagaca attcagtcac gtgaacaagt ccgtcaacac
     9421 caccaaggtc aatgaaagca ccaaaacttg ttaaacgcgc tacagtacct gtaacgactg
     9481 caccttctga aatcttagag aacacttcag cacgagcttc tttagcggcc tcttcgataa
     9541 cttcacgacg tgataggatg aaacggtttt ctgctgcatc aacttctttg attttagcat
     9601 caaactcttg tccaacaaat ttttcagtgt tgcgaacaaa acgagtgtca atcattgaag
     9661 cagggataaa tccacgaagt ccttcaaatt caactgaaag gccaccttta acagcacggg
     9721 tacctttaac agtaacaact tcgccttcac gaccaacaag tttgtcccag gctttacgag
     9781 cttccaaacg ttttttagat actaggaaag taactgtatc agtatcttta cctactactt
     9841 gacgaagaac aagtacttca actgtgtcgc cagctttaac aaagtcgtta atatcagcat
     9901 cgcggtcgtt agtcaattca cgaagtgtca agacaccttc aacacctgtt ccttcgataa
     9961 caacgtttgc ttgaccgtta tcaacagtta aaacttccgc agtgacaaca tcaccagggt
    10021 tcacttcgct aacactgttt agcaaatctt caaattcatt cattctaaaa aatcctccaa
    10081 caaaagctag ccaatgctag cttttgataa caatatattt tctcaaggta acccgcaacg
    10141 acaacaactc ttatctttga cggtctcagg gagcacctga tacctctgac tggggtagct
    10201 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctata
//
LOCUS       DAUCWU010000057         9956 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUCWU010000057 DAUCWU010000000
VERSION     DAUCWU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.057"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..702
                     /locus_tag="VGY24_001271"
     CDS             <1..702
                     /locus_tag="VGY24_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ4284250.1"
                     /translation="IKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEI
                     VVSLDVLPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQH
                     WPAIVYRLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLS
                     LKPRSFEMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMK
                     AKRIKQDATGTELIG"
     gene            775..1284
                     /gene="msrA"
                     /locus_tag="VGY24_001272"
     CDS             775..1284
                     /gene="msrA"
                     /locus_tag="VGY24_001272"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ4284251.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            1281..1496
                     /locus_tag="VGY24_001273"
     CDS             1281..1496
                     /locus_tag="VGY24_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ4284252.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            1652..2821
                     /locus_tag="VGY24_001274"
     CDS             1652..2821
                     /locus_tag="VGY24_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ4284253.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMASSATPSDVPTTPFASAKPDSSVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPVQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            3136..4908
                     /locus_tag="VGY24_001275"
     CDS             3136..4908
                     /locus_tag="VGY24_001275"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ4284254.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWVYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEGKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKENETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            5067..6119
                     /locus_tag="VGY24_001276"
     CDS             5067..6119
                     /locus_tag="VGY24_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ4284255.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            6165..6740
                     /locus_tag="VGY24_001277"
     CDS             6165..6740
                     /locus_tag="VGY24_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ4284256.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            6899..7396
                     /gene="ybeY"
                     /locus_tag="VGY24_001278"
     CDS             6899..7396
                     /gene="ybeY"
                     /locus_tag="VGY24_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ4284257.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            7377..7784
                     /locus_tag="VGY24_001279"
     CDS             7377..7784
                     /locus_tag="VGY24_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ4284258.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNFRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            7904..8800
                     /gene="era"
                     /locus_tag="VGY24_001280"
     CDS             7904..8800
                     /gene="era"
                     /locus_tag="VGY24_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ4284259.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            8820..9296
                     /locus_tag="VGY24_001281"
     CDS             8820..9296
                     /locus_tag="VGY24_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ4284260.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILINDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         3036 a         1822 c         2155 g         2943 t
ORIGIN      
        1 ataaagcaaa aggcacgtct cacaaccaaa gagatccgtt caacacggac aagttatggc
       61 tggggtgaag tgacagaggt tcgtcgtgac ctaggcgttt ttgtcgatac aggaattcca
      121 aacaaagaaa ttgtggtttc tttggacgtg ttgcctgaaa tgaaagaact atggcctaaa
      181 aaaggggata aactttacat tcgtttggat gtggataaaa aagaccgtat ttggggactt
      241 ccagcagagc ctgaggtctt tcaaaagatg gctagccctg cttacaataa catgcaaaat
      301 caacattggc cagcaattgt ttaccgccta aaattaacag gaacttttgt ttacctgcct
      361 gaaaacaaca tgttgggctt tattcaccca agtgaacgct acgcagaacc gcgcttaggt
      421 caggttttag atgcccgtgt gattgggttt agagaggtcg atcgtacctt aaacttgtcg
      481 ttgaaaccac gttcttttga gatgttggag aatgacgctc agatgattgt cacctatctg
      541 gaagccaatg gtggtttcat gaccttaaat gacaaatcaa gtccagaaga gattaaagca
      601 acctttggta tttcaaaagg ccagtttaaa aaagccttgg gtggattgat gaaagctaaa
      661 cgcatcaaac aagacgccac aggaactgaa ttaataggat aaaaatgctt tgccactggc
      721 aaagtttttt gttacaatga agaccattaa gtgaagaaaa aggggatata gagaatggaa
      781 agagccattt ttgcaggagg ttgtttttgg tgtatggtgc aaccttttga ggagcaagca
      841 ggtattttgt cagtcagaag tggctacacg ggtgggcatc tcccaaatcc tagctatgaa
      901 caagtttgtg ccaaaacaac aggacataca gaagctgttg aaattatttt tgaccctaag
      961 caaatcgctt ataaagattt ggtagagctc tattgggcgc agacggatcc aacagacgcc
     1021 tttgggcaat ttgaagatcg cggggacaac taccgtcctg tcatttacta tacaacggaa
     1081 agacaaaaag aaattgcaga gcaatcaaaa gcaaacttgc aagcttcagg acgttttgac
     1141 caacccattg tcaccactat tgaaccagca gagccatttt atctcgcaga ggattatcac
     1201 caaggatttt ataaaaagaa tcccaagcgg tatgcacaaa gcagtgctat tcgtcatcag
     1261 tttttagagg agaattggtc atgagaaaat cattttacag ctggctgatg acacaacgta
     1321 accctaaatc aaatgaacct gtggcgattt tagcagattt ggtgtttgat gacaccactt
     1381 ttccaaagca taccaatgat tttgaactca ttagtcggta tctagaagat caagctagtt
     1441 tttcctttaa tttaggtcaa tttgatgaaa tctgggaaga ttatctagca cattaatacg
     1501 tggataaaaa agagtcatat cgactctttt gaagggttat cacgttatta ttacaaaatg
     1561 acatcacttt ctttcaaaaa aatgacaagc tacttttttt cttaaaaata tgctatgata
     1621 tagcttagaa aaaaataaaa aggaagcaga gatgattatt actaaaaaga gcttatttgt
     1681 gacaagtgtc gctttgtcgt tagcaccttt agcgacagcg caggcacaag agtggacacc
     1741 acgatcggtt acagaaatca agtctgaact cgtcctagtt gataatgttt ttacttatac
     1801 tgtaaaatac ggtgacactt taagcacaat tgctgaagca atggggattg atgtgcatgt
     1861 cttaggagat attaatcata ttgctaatat tgacttaatt tttccagaca cgatcctaac
     1921 agcaaactac aaccaacacg gtcaggcaac gactttgacg gttcaagcac ctgcttctag
     1981 tccagctagc gttagtcatg tacctagcag tgagccatta ccccaagcat ctgccacctc
     2041 tcaatcgact gttcctatgg catcatctgc gacaccatct gatgtcccaa cgacaccatt
     2101 cgcatctgca aagccagata gttctgtgac agcgtcatct gagctcacat cgtcaacgaa
     2161 tgatgtttcg actgagttgt ctagcgaatc acaaaagcag ccagaagtac cacaagaagc
     2221 agttccaact cctaaagcag ctgaaacgac tgaagtcgaa cctaagacag acatctcaga
     2281 agattcaact tcagctaata ggcctgtacc taacgagagt gcttcagaag aagtttcttc
     2341 tgcggcccca gtacaagccc cagcagaaaa agaagaaacc tctgcgccag cagcacaaaa
     2401 agctgtagct gacaccacaa gtgttgcaac ctcaaatggc ctttcttacg ctccaaacca
     2461 tgcctacaat ccaatgaatg cagggcttca accacaaaca gcagccttca aagaagaagt
     2521 ggcttctgcc tttggtatta cgtcatttag tggttaccgt ccaggtgatc caggagatca
     2581 tggtaaaggt ttggccattg attttatggt gcctgaaaat tctgctcttg gtgatcaagt
     2641 tgctcaatat gccattgacc atatggcaga gcgtggtatt tcatacgtta tttggaaaca
     2701 gcgattctat gcgccatttg caagtattta cggaccagcc tacacatgga accccatgcc
     2761 agatcgcggc agtattacag aaaaccatta tgatcatgtt catgtctctt ttaatgctta
     2821 attcatgcta tcaaaaaaac tcacgtttag gcgtgagttt tttgacaatt ctgacagagt
     2881 aagccctata agaggctgag atacgctttt aatacctgtt gtttttctca ccctttagac
     2941 agttttaacg ttatgtctaa aagagatggt ttttattagc gcaaatatgg gtttaggggt
     3001 aaaatatagg taattaacct gatatgatca tgataaaggt agaagaatac ctattatttc
     3061 gtgacaactg agcaatctgc ccttaccttt ggagtgatga tatcactttg cttgactatt
     3121 caaggaggtc ttgaaatgta ttatactagt ggtaattacg aagcttttgc gacacctcga
     3181 aaacctgaag gggtagatca gaaatcggct tatattgttg gtactggttt agctggttta
     3241 gcagcagctg ttttccttat tcgcgatggg catatggctg gggaacgcat tcatctgttt
     3301 gaggaattgc ctttagcagg tggttcttta gatggtattg aaaagcctca tcttggtttt
     3361 gtgacccgtg gtggtcgtga gatggaaaat cattttgagt gtatgtggga catgtatcgg
     3421 tctattccct cactggaaat tcctggtgcg tcttatttgg atgaatttta ttggttggat
     3481 aaggatgatc ctaactcatc caactgtcgt ttgattcaca agagaggaaa tcgtgtggat
     3541 gatgacggcc agtatacgct cggtaaacag tcaaaagaat taatccattt aatcatgaag
     3601 acagaagaat ctctaggaga tcaaaccatt gaagagttct tctcagaaga tttctttaag
     3661 agtaattttt gggtgtattg ggcaaccatg tttgcttttg aaaaatggca ttctgctgta
     3721 gaaatgcggc gctatgcgat gaggtttatc caccatattg atggtttgcc agatttcacc
     3781 tctctcaagt tcaacaaata taaccaatat gattctatgg tcaaaccgat tattgcttac
     3841 ctagaatcac acgacgttga catccaattt gacacaaaag tcactgatat tcaggtggaa
     3901 caaacagctg gtaaaaaggt agcaaaaacc atccatatga cggtgtctgg ggagggtaag
     3961 gcgattgagc taacacctga tgatttggtt tttgtgacca atggttctat tactgaaagt
     4021 agcacatacg gtagtcatca cgaagtggct aagccaacca aagcgttagg tggttcttgg
     4081 aatttatggg aaaatctagc tgctcaatca gatgattttg gtcatcctaa agtgttttat
     4141 caggacttgc ctgctgaaag ctggtttgtg tctgctacag caaccataaa acacccagct
     4201 atcgagcctt atattgaacg tttgacccac cgtgacttgc acgatggcaa agtgaacact
     4261 ggcggcatca tcactattac agattctaac tggatgatga gctttgccat tcaccgtcaa
     4321 cctcatttta aagaacaaaa agaaaatgag accactgtct ggatttacgg tctttattcc
     4381 aatagtgagg gcaattacgt ccacaagaaa attgaggagt gtacaggtca agaaatcaca
     4441 gaagaatggt tgtaccacct tggggtacct gttgataaaa tcaaggactt agcgagtcag
     4501 gactatatca atacagttcc tgtttacatg ccttatatta cgagttactt tatgccacgc
     4561 gtcaaaggag accgtccgaa agttatccca gatggttcag tcaacttggc ctttattggt
     4621 aactttgcgg aatctccatc tcgagatacg gtctttacga ctgagtattc tattcgtact
     4681 gccatggaag cagtgtatag cttcttgaat gtggaacgag gcatcccaga agtctttaat
     4741 tcagcctatg atattcgtga attgctcaaa gccttttatt accttaatga taaaaaggca
     4801 atcaaggata tggatttgcc aattcctgca ctgattgaga aaatcggaca taaaaaaatc
     4861 aaggatacct ttatcgaaga attgctcaaa gatgctaatc ttatgtaaca tgccttagag
     4921 tagttaaagc tgataagttt aggcttatca gcttttttga tgatttatag aaaagtgata
     4981 aaggaaaagt ctgacgtgaa cgtcacataa gaatttaaaa tttgttataa tgtaagcaat
     5041 aaagatgaaa gaaagaggga ctctatttgc aagagtattc tattgacatt accttaaccc
     5101 atcctgatga tgttttagct ctttttggta gcaacgagcg tcatttgaag ctaatagaag
     5161 ctcatttagg agttattgtt cacgcaagaa ccgagcgagt ccaagtgatt ggtgatgatg
     5221 aagaagctgt ggaattggct cgattgacca tcaaagccct actggttcta gtgggtcgtg
     5281 gcatggtggt caacacatca gatgtggtga cagctttatc tatggcagaa tctcatcaga
     5341 ttgaccaatt tatggcgctt tatgaagaag aaatcattaa agataactat ggtaaggcaa
     5401 tacgagtcaa aacattaggt caaaaaacct atgttgatag tgtcaaacgt catgatgtgg
     5461 tttttggtgt aggacctgca gggacaggga aaaccttctt agctgttacg cttgcagtca
     5521 ctgctctgaa aagaggacag gtaaagcgta ttattttgac gcgtcctgcg gtagaagctg
     5581 gtgaaagcct aggtttttta ccaggcgatt tgaaagaaaa ggtggatcct tacctcagac
     5641 ctgtttacga tgccctttat cacattttag gcaaggaaca aaccacacgc ctgatggaac
     5701 gagatgtgat tgagattgct ccgttagctt atatgcgggg tcgtactctg gatgacgctt
     5761 ttgttatctt agatgaggct caaaacacga ccatcatgca aatgaagatg tttttgaccc
     5821 gtcttggttt taattctaaa atgattgtca atggagatac gagtcagatt gacctccctc
     5881 gaaatgtcaa gtctggtttg attgatgcta ctcagaaatt acaaggcatt aaacagattg
     5941 attttgtcta tttctctgct aaggatgttg tccgacatcc tgttgtggct gatattatca
     6001 aggcttatga aacgtcctca gaggagatga aggacttact aaaacctaag gctagtgacg
     6061 ttgaagctga ttaccagcca ggcttgacaa aataccctgt gatcggtcaa gagcactaag
     6121 acttttacga tgatgtttaa tatggccttt ttaaggagga ttctatggat gatttaacca
     6181 aagctattat ggcagatgag gctaatctct cctataccga aagaggtatt tttcctttat
     6241 atgatgctcc tcaaacagct aggattatta ttgttggcca agctcctgga atagtagctc
     6301 aagaaaccaa actttactgg aatgatcgta gtggcatccg cttacgagac tggctaggtg
     6361 tggacaatga caccttttac cactcaggat tgtttggcat tatccctatg gatttctatt
     6421 atcctggtaa gggaaaatcc ggtgacttac cacctagaga aggttttgcg gctaagtggc
     6481 acccgccatt aagagcattg atgccagaag ttgaattgac gattttggtt ggtcgctatg
     6541 ctcaggactt ttaccttggt aacaaggctt acaaaacctt aactgaaaca gtccgtcact
     6601 ttgaggatta cttgccagac tattttccac ttgttcatcc ttctccaaga aaccaattgt
     6661 ggttagctaa gaacccctgg tttgagcagg atttattacc tatcttgcaa aaacgtgtgg
     6721 aggcaattct aaccaaatag gccctgattt acctagcatt cccattttta aggggcttgt
     6781 ggtataatgg gtaaataaca aatgctgatt gaaaggataa gggaaattat ctgtcagata
     6841 ataatgggtg cccaacatac caaatgaggt gggcagccct tccctaacaa ttaaccttat
     6901 gtatatcgag atgattgacg aaacgggaca agtttcgcaa gagattatgg agcaaacact
     6961 tgatttgctc aattttgctg ctcaaaagac gggcaaagaa gaaaaagaaa tgtctgtgac
     7021 ctttgtgacc aatgaaagaa gtcatgaact caacttagaa taccgagata ctgatcgtcc
     7081 aacagatgtg atttctctag aatataagcc agaaacgcct attttattta gtcaagagga
     7141 tttggctgct gatcctagtt tggcagagat gatggcagag tttgatgctt atattggcga
     7201 attgtttatt tctattgaca aggcgcgtga gcagtcccaa gaatatgggc actcttttga
     7261 gcgtgaaatg ggatttttag ctgttcatgg attcttacat attaatgggt atgatcacta
     7321 cacccttgaa gaagaaaaag agatgtttac cttacaggaa gagattttga ctgcttatgg
     7381 ccttacacga caataatacc accaaacgga aatggaaaaa cagaaccatt acctcaagct
     7441 tggagtttgc cttgacaggg gttttcacag cttttaagga agaacgcaat ttcagaagtc
     7501 accttttgtc tgcctgttta gcttgtgttg ctggtttatt ttttagcatt tcagccatcg
     7561 aatggttatt tttacttcta gccatttttt tggtcattac cttagaaatt gtcaattctg
     7621 ctattgaaaa tgtagttgat ttggctagtg actatcactt ttctatgcta gctaaaaatg
     7681 ccaaggatat ggcagcaggt gctgttttga tgatttctgg ctatgctgtc ttgacaggac
     7741 ttattatttt tattcctaaa atttggaata ttttcgttca ttaaaaagaa aggaatgggc
     7801 tatcctatgt gatgatggca taggagaata aggaaaggac aagacggtta agcttagtga
     7861 caagcttgat ctgaagccct accgttaagg cccgtaatta tctatgttta aatcaggctt
     7921 tgtagccatt cttggtcgtc caaatgttgg caaatccacc tttttaaatc acgtcatggg
     7981 gcaaaaaatt gccatcatga gtgacaaggc tcagaccacc cgtaataaaa ttatgggcat
     8041 ttacaccaca gaaacagaac aaattgtctt tattgatacg ccaggtattc ataaaccaaa
     8101 gaccgccctt ggtgatttta tggttgaatc tgcttatagt actttacgtg aagttgagac
     8161 agttcttttc atggttcctg ccgatgaaaa gcgtggtaag ggtgacgaca tgattatcga
     8221 gcggctaaag gcagctaaga ttcctgttat tttagtcatc aacaaaattg ataaggttca
     8281 tccagaccaa cttttggaac agattgatga cttccgtagc caaatggatt ttaaagaagt
     8341 ggtcccaatt tctgccctag aaggtaataa tgtgccaact ttgatcaagt tattaactga
     8401 taatcttgaa gaaggtttcc aatattttcc agaagatcag attactgatc atccagaacg
     8461 tttccttgtg tcagaaatgg ttcgtgagaa ggtactgcat ttgacgcaac aagaggttcc
     8521 acattccgta gctgttgttg tcgaatccat gaagcgagat gaagagactg ataaagtgca
     8581 tatccgtgca accattatgg tggagcgcga tagccaaaaa ggcatcatca tcggtaaaca
     8641 aggagccatg ctcaaaaaaa tcggaaaaat ggcgcgccgt gatattgaac tcatgttagg
     8701 tgacaaggtc tacttagaaa cgtgggtcaa agtcaagaaa aactggcggg ataaaaagct
     8761 tgatttagct gattttggct ataatgagaa agaatactaa catagtaaag cgagatcata
     8821 tgccacaaga ctacatttct tatatccgct ccaaagttgg gcatgataag attattctta
     8881 actttgctgg tgggattttg atcaatgatg acggcaaggt gctgatgcag ttgcgtggtg
     8941 ataagaaaac ctgggctatt cctggtggca ctatggaact gggtgagagc tctttagaga
     9001 cttgcaaacg tgagttctta gaggaaaccg gaattgaggt tgaagctgtt cgcttactga
     9061 atgtttacac tcattttgag gaagtttatc ccaacggtga tgctgttcag actattgtct
     9121 ttatctatga actaacggct gtttctgata tggctattga taactttcat aatgaagaaa
     9181 cgctcaagtt acagtttttc tctcatgagg agatagcaga gttagagagt gtctcagcca
     9241 aacatcgcct gatgttagag gaatatttta gtgatagctt tgctatgggg cattaagaac
     9301 tctctaggac agttggagta tttttggaga ttggcttgtt aaaaaaattt ttgaaagacg
     9361 atgtttcgag agcttatgct ctcttttttt gcttctaatt tttctgatta agtctgaata
     9421 attttgttaa atgttaacag gattttcttt ccaattgata ctagcagaca tcaaacattt
     9481 gttttagagt tatgacgaat tgccacctag ccatgcatga cttaggcaag ttgaacctaa
     9541 ctgtggcaat agacttggaa ggggtgacaa gaatcatttc ctaaaagaat tgatgaagtg
     9601 cctatccatt attcgaattg taaagttcgt ctaagtaaag ttgaatttgg tctcgaatgg
     9661 accttaatga tttatgactt gttgtgtcaa taacaccaaa aactcgataa tgatttttaa
     9721 atatttcctc gtaattaata tgatttccga ttagaataat atgagcatga ggataacgca
     9781 tacgtatttc gtttagaagg tcatatactt gctcagattt aaaaaagtat gatttatgat
     9841 aaaataaacc tgacatttgt atttataatc taagtttttc ccactaataa tttgagtttt
     9901 tagaaagtat ctcttgaggt aatagttaat atagatagat atcatatatc ctaaaa
//
LOCUS       DAUCWU010000058         9871 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUCWU010000058 DAUCWU010000000
VERSION     DAUCWU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.058"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            17..2290
                     /locus_tag="VGY24_001282"
     CDS             17..2290
                     /locus_tag="VGY24_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ4284261.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2407..3486)
                     /locus_tag="VGY24_001283"
     CDS             complement(2407..3486)
                     /locus_tag="VGY24_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEQ4284262.1"
                     /translation="MKNISRKCFMTSVVCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPIVTTVTGKTLSLSQKP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKASNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTISLGN
                     GQLTTKDTTLKAVNIQSLHPGGIEAERTTLENVTFTVSKSKEEEENDYYDNDAIFTAH
                     ALTLKGTNTITGGDIDVDITLTKAKAIAYRARTENGKVSLGSQLTPAKIGKESTSDVI
                     SYVAENKAATGNLTVNLNKGDITIK"
     gene            complement(3483..4070)
                     /locus_tag="VGY24_001284"
     CDS             complement(3483..4070)
                     /locus_tag="VGY24_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ4284263.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(4057..4383)
                     /locus_tag="VGY24_001285"
     CDS             complement(4057..4383)
                     /locus_tag="VGY24_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ4284264.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
     gene            4590..5231
                     /locus_tag="VGY24_001286"
     CDS             4590..5231
                     /locus_tag="VGY24_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEQ4284265.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWESIQPNEEVDKVFTVSLRQLLVTDPVYYHLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(5278..6120)
                     /locus_tag="VGY24_001287"
     CDS             complement(5278..6120)
                     /locus_tag="VGY24_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ4284266.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
     gene            6537..6581
                     /locus_tag="VGY24_001288"
                     /pseudo
     CDS             6537..6581
                     /locus_tag="VGY24_001288"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6588..6869
                     /locus_tag="VGY24_001289"
     CDS             6588..6869
                     /locus_tag="VGY24_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ4284267.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(7345..7947)
                     /locus_tag="VGY24_001290"
     CDS             complement(7345..7947)
                     /locus_tag="VGY24_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284268.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(7944..8696)
                     /locus_tag="VGY24_001291"
     CDS             complement(7944..8696)
                     /locus_tag="VGY24_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ4284269.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGRQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            complement(8762..9535)
                     /locus_tag="VGY24_001292"
     CDS             complement(8762..9535)
                     /locus_tag="VGY24_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284270.1"
                     /translation="MLYLNTQTDSDYKEIIFGDIAKFVENMFYHYFSSILFRDLETVD
                     KRMYSFSDDDLISIQSSCLRLSKTFANFNIQRKNFLASDETEMDTFHKKDDIDITVGE
                     KSYNLARSFRTSTINELTVFNFEEMFDIIWLMLGDNLIKSFEVNVCGILFELDGNGIP
                     STFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFLVDDILERMYKENIPK
                     SYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            complement(9729..>9871)
                     /gene="cadX"
                     /locus_tag="VGY24_001293"
     CDS             complement(9729..>9871)
                     /gene="cadX"
                     /locus_tag="VGY24_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ4284271.1"
                     /translation="NLSMASASHHLRKLANQNILGTRREGKIIYYFIKDEEIRDFFNQ
                     LG"
BASE COUNT         3141 a         1740 c         1922 g         3068 t
ORIGIN      
        1 cattttagga gaaaatatgt tagaagaatt gaaaacactt attaaaaatc caaaattaat
       61 gattacaatg attggtgtgg ccctagtgcc tgccttatat aatttatcct ttctaggctc
      121 aatgtgggat ccttatggtc gggtcaatga ccttcccatt gctgttgtta atcatgataa
      181 gcctgcaaag agagctgata agtcattgac aattgggaat gatatggtgg acaagatgtc
      241 taaaagtaaa gatttagagt atcattttgt ctcagctaag caagctcaag agggacttaa
      301 agaaggtgat tattatatgg ttattacttt acccgaagat ctttctcaac gggcagcaac
      361 cttattaaat cccgaacccc aaaaattaac tatccgttac caaacgagta aaggacatgg
      421 aatggtcgct gctaagatgg gggaaacagc gatggctaag ctgaaagagt ctgtttcgca
      481 aaacattacg aagacttata cctcagcagt ttttagcagt atgacagacc tccaatcagg
      541 attaaaagaa gcctcagctg gaagtcaagc acttgcttca ggagcgaaga ccgctcaggc
      601 ggggagtcaa acactttcga cgaacttagc agccttaacg ggtgctagcc aacagtttca
      661 acaaggtact ggtcgattga catcaggttt gactacctat acagatggtg tcaaccaagt
      721 caagaatggg ttaggaacat tatcaacgga catccccaat tatctgaacg gggtttctag
      781 gttatcccag ggagcttctc agcttaatca gggtctttca cagttgacac aagcaacaac
      841 actttctgac gagaaagcta agggaattca atccttaatt gtaggactac cagtcctaaa
      901 tcaaggcatt cagcaactaa atacagagct atcaacattg caacccccta accttaatgc
      961 tgatgagtta ggtaatagct taggagctat cgctcaagct gccaaacaag ttattgctga
     1021 agagactgcc gctcagaatg aacaactatc ggctctccaa gccactagtg tttatcaatc
     1081 attaactgct gaacaacaag gagagttagc tgcggccctc agtcaatctg ataaaagtca
     1141 aaccgtatct gcagcccaaa ctattttaag ttctgttcaa acactgtcaa caggtttaca
     1201 aactctgtcc caaggaaata gcttagctca attagaccag cttaaagcag ctgtttcaca
     1261 gattgcgagt cagtctaacc aggctttacc tggagcgagt tctgcattat ctgagttgtc
     1321 aatggggtta tcaaaggtaa atggtagctt aaatcaacaa gttctaccag gaagtaatca
     1381 attgacaaca ggattagcac aattaaacag gtataatact gtcattggtt ctggggtaat
     1441 aaaactctca gaaggtgcca atgccttgtc atccaagtcc ggagaattac tagatggtag
     1501 ccatcaatta tcagagggtg ctactaaact agctgatagt agttctcaat tgagtcaggg
     1561 tggtcatcaa ttaacgagcg gattgactga attatcaaca ggattatcaa ccttaaatgg
     1621 ttccttagcc aaagcctctc agcagttatc gcttgtttct gtgactgata aaaatgctaa
     1681 agctgtcgca aaacctcttg tgttaaatga gaaagacaaa gatggtgtta agacgaatgg
     1741 gatcgggatg gcaccttata tgattgctgt ttctctaatg gttgtggccc tttcaaccaa
     1801 cgtcattttt gctaattctt tatctggtcg tccggtcaaa gataaatggg attgggctaa
     1861 acaaaaattt gttattaatg gttttatttc gactatggga tccattgttc tctacttagc
     1921 tattcaatta ttagggtttg aagcccgtta tggtatggaa accttaggat ttattatgct
     1981 aagtggttgg acgtttatgg ctcttgtcac agctttggtc ggttgggatg atcgatatgg
     2041 ctcttttgct tctttggtca tgttattact tcaggttggc tcttcaggtg gctcttaccc
     2101 cattgagtta agtggagcat tcttccaaaa gttacatcct ttcttaccaa tgacttatgt
     2161 ggtatctggt ttacgacaaa ccatttcatt atcaggtcat attggagtag aagtgaaagt
     2221 cttaactggt ttcttactgg catttatggt attagcacta ctcatttatc gtcccaagaa
     2281 aacagtctaa ataaaataac ctcaaaaaaa acagcatcta aggcagttac actagatgct
     2341 gttttttgtc ttattttgac aataacttga caatttctgt caagtttttt tattgtcttg
     2401 ttatgattat ttgatagtaa tgtctccctt attgagatta accgttaaat ttccagtagc
     2461 tgctttattc tcagccacat aagaaataac atctgaagtt gattccttac caatcttagc
     2521 tggtgtcagc tgtgagccaa gggagacttt accattttcg gtccttgctc tgtaggcgat
     2581 ggcctttgct tttgtcaagg ttatgtcaac atcaatatca ccaccagtaa tagtattagt
     2641 ccctttaagg gtaagggcat gagcggtgaa gatagcatca ttgtcatagt agtcgttctc
     2701 ctcttcttct ttgcttttag aaacagtgaa ggtcacattt tcaagggttg ttctctcggc
     2761 ttctatacca ccagggtgta atgattgaat attgacagct tttagagtgg tatctttagt
     2821 agttagttgt ccatttccta agctgatggt cccattttcc acactagaat cagtcaggtt
     2881 tgaaaaagaa tagtcagctt ttaactcgag atttgttaac tgacttttag tggcttccaa
     2941 cataccagtg atagtagctt tcttgacttt ggtatgtaaa agagtaagat tgccatcgaa
     3001 ttgcatatct tggacagtta gatttgacaa agtggtatgt gggacattac ttgccttaac
     3061 ttcattgagg ctagtttttt caggaacagt aatggtaata gaacggtagt tcttctcttg
     3121 acgactatta ttgagagtaa aacctaaaat ttcaattcca ccagtgatga caacgtcttt
     3181 aggtttttgc gaaagagata gggtcttacc tgttactgtt gtgacgatag ggtcaataaa
     3241 tttaggatgt gtgtaatagg tcactgttgg tctttgaacg ggtcctgtct ctactgtaat
     3301 agttgaggca gaactgtcaa tatcaagagc agttatttgg tcaaatgttt tgatgacctt
     3361 tttgggagct gtttggtgtt taatgtcctg aagtcctcca gttgcatagc cagcccctag
     3421 aagaatgcct cctagaataa tacataccac actggtcata aagcatttgc gtgagatatt
     3481 tttcatgctt gagctccttt cttattactg atccacttaa taaaggcaac aaaagcccgt
     3541 cctgaaaaac gaaggattgc tgtggcaata atccaaataa gaagaccacc accaaaacac
     3601 aacaaggcgc tgccgatgcc catagcttgt gcaggaatag atgagccaat caaggtcaag
     3661 ctttcaaaca gagtcactcc tgcgacaaga atacctgcaa gcccaaaaat gtaggcggca
     3721 aagatagctg tgagcccgag cattaggcag gcaataataa agagaatcaa cgccagcaat
     3781 acgggtaaag caatcggagc agcaaagagg gacaaaatgg ttaatccaat aattgttgcc
     3841 cgatttttgg gagttttctc gggagttgcc aagtgttttc ctagaacatt tgaaatgatt
     3901 tcactggcag cttccttagg atttcctaaa tcatcgataa cctggctttc tttgtcagga
     3961 ccagcttcat caaaatattc aatataatat tccatcgctt catggtagtc ttctctaggt
     4021 agtttgcgca agtatttatc taattctgct agatactcag tccttgtcat gtcttaagct
     4081 cccttcaata atcccgtcta aggcaaattt atagctctgc cattcttttt ttagaaaaat
     4141 cagctgtgct ctccccgaag aagtgacagc ataatattta cgctttcgtc cctgatgttc
     4201 ttgactataa gttgtcaaaa aacctgcctt ctccaatttt ttaagaatag gatagagggt
     4261 ggattctttg atattagcca ctagtttaat ggtttgacta atatcgtaac cgtaagaatc
     4321 atttttttcg acaatggcta aaatcagaaa ttcaatcaag gtagccgata ctggaaaata
     4381 cattttaggc tccttccata tataaaaatt ttatatataa ttattttaca tatttagtat
     4441 aagcttagtt ttttgatatg tcaatatttt atatataaaa aaataatata tatatcaaaa
     4501 atcactaact aggagtaata agttgaccaa gaagctagga ttggttatga tgaagataag
     4561 attaacagaa agagaaatta gggaaggcta tgaaggactt attaaaacag tatcaggcaa
     4621 aaccactagg agaggagaag cggtacgccg tatttttacc tcttattctt gtcaatgatg
     4681 actggcatgt cttatatgaa gtcagaagtc agcatatttc acaaccgggg gaagtttctt
     4741 tcccaggggg acaagtagaa aatcaggaga cacttcaaga agctgctatc cgagaaacag
     4801 ttgaggaatt gactgttgat gcgtcccaga tccagctatg gggtgaaatt gattatttag
     4861 tacagtcttc tagaactatt cattgtttcg ttggtcaatt agtcgttgat gactgggaaa
     4921 gcatacagcc taacgaagaa gttgacaagg tctttacagt atctttacgt cagctacttg
     4981 tcactgatcc tgtctattat catttagaag ccactcctat tgagacaacg gattttcctt
     5041 ttgaccgtat tcgtaatggg aaatactacc aatttagtca agagtaccgt agtattccgt
     5101 tttatgaaaa tctagaagaa accatatggg gaatgactgc ccagtttact aagtgtttaa
     5161 ccgatattct aaatgatgaa tcaattgcaa agaaaaaccc cttagatatt gaaaaatcta
     5221 aggggagata gagcactagt ttattggcgt caactttttg tcaacaacct gtaaaggtta
     5281 tttgtatttt tcttctaatt gactcatcca aagtccgagc caaataccaa gagcaaataa
     5341 cacaaaggcc ataagccagg ttaagatacc tgcatgactg taggaaatgg attcgggact
     5401 actactattt ggaataagaa ttaatagcgt gctggataat acaataccaa tgataaagtg
     5461 gtaaactttt gaatgatggt gctgtaaagc ataatccata cttttagaaa aagccaagat
     5521 ggctaggact cctccgatag caataggtaa gaaagtccct aataaatcca aagatttgaa
     5581 tcctattaac ataggaccat agagtcctaa gataagtaag agatttgacg ggcttagtcc
     5641 agggaccagt actcctaaag caattaaggc acctgctaaa ataaaggtta aaaagttagc
     5701 tggtaaggta ccgattaagt catttaagaa gtaaagacct agtcctgaaa taacaaaggt
     5761 aaggactagc catagccagt caactttgtc tctttgagac tgcttggttg actccttaat
     5821 cagactagga atagtgccga caatagcgcc agcaaatccc cataagacga taacttgata
     5881 gtgcttaagc aaaaactcaa cggggaaaga aaagagagca atacctaaaa taccaccaat
     5941 tcctactggt aagaaaaata agacattttc aataaagtta tccctcatat gagctaaaaa
     6001 ggaaatcatt cgctcataga ttcctaaaat ggctgcgaga acaccaccgg atacaccagg
     6061 taaaataaaa cctagtgcaa taatcatgcc tttaaaaact cttgaaataa atgaaaccat
     6121 tgttgcctcc tgaaaagaat gattttattg tatcataaat ttaaagaatt gctgacacaa
     6181 agctgcatat ccctactaaa ataattgttc ctaataaata atacattata aataatacat
     6241 tcaattttga agatattttc cttaaacacc atgtctttgt gatcaagtca agtttgattg
     6301 tcaaagttgt aaataaattt tacaaagact gtaaacctta cgataattat caaaaagata
     6361 aacgaaaaaa agcttaggtc aaagactttt gatagtaaac ttattgaaga aggattatag
     6421 tagctattaa aaaggagatt tattatgggc tttaaaaacc agttacctta ttattgaaaa
     6481 aaattacaga tgtcacaaga agagcttgct gaaatgatgt atgtttcaag aagactatct
     6541 caaactggga aaacggtaaa agctatccag acctgcaaaa tcttttgatg ttaagtaagt
     6601 ccttacaggt tcctttagag caactaatag cagaagatat cccaaaaatg actttaatta
     6661 ttaaggatga ggagattaaa caatgtgata ggaatacgca tattatgtta tttgggacac
     6721 tcctaattgc tatcagtatt tatcctataa tgtcttggtt aggttggtgg ggatatggtc
     6781 cggttgctat cttatggttg ataatcatgc gtagcgcctt aagaattgag cgatttaaaa
     6841 agacatatga tgttcagact taccgataac ttgaagctat agcaaagggt gagagcttag
     6901 atcagattga taagattaaa gaaaaagcaa aatatcctta tcaaaaagca tggattgtta
     6961 ttgcgtacac aggattgaca gtgttaataa ccctactctc tattttgttt tttcatttgt
     7021 ttttttaaac acgaaaaaag cctttgaaac gttcatttca taggcttttc tcatctcaat
     7081 tattttactt ctgtaaaggt taagtatttt ctattttagc tgttataaac cttattgtat
     7141 caatatttat tgatagttgc ttaataaaag gtagagtgaa tggtagagta gttattgcgt
     7201 tagttcagca atcttttcaa gataaataaa tacccacagc ttctaacttt cgttgtcgta
     7261 gtaaccccaa taggtcatga tagataagaa tataaaaaag gggctgtctg gcttttgctt
     7321 aacgcaagcc acagccccaa ggaattaacg cttttgacca ataaaccaca attggttacg
     7381 aactcgaaca ggagaagaga aataatagtc tgtgtgttgg atgacaatct cttctttcag
     7441 ttgtactccc gtatccttta ggattttttg cccctgttgg tctctaggaa cttttagaaa
     7501 tacagtaatg gcatcttttc gtatatctac gatacactta cgtacagctc ggttaaaccc
     7561 gttatggatt ggattgatag tggtaacggt ctggtgttca aagttgctta ccttctgtac
     7621 tcgttcacgc tggaaaagga agtgtcttaa tagaaaggtg cgataaatag attggcagta
     7681 gttcccaagg ctatctgtat tcaagcgttg tatcatcttt aatccagata ataaggctag
     7741 aagtaaagaa ataagagcga atccataaca tacagaacta atggtatcag taacgttttt
     7801 aaccgtatcc agattgacaa atgtgacggt agataattga actgaaacaa gattgaacag
     7861 ccaaccgaac acatagcaga ttggagcaat aagcagtaag tataatatga gattgaagct
     7921 tctcatgaag taggttttag tggtcatagg atacctcgct tagtatagta ggttggacag
     7981 agtaagggta gaacttgata ggagtgttca ttatcgacaa cttgaatgat acctgttccg
     8041 tgtcctgttg gaatgacaat cccctctgga tctaggtctg gaaataagaa ttgagtggtt
     8101 ttctgattga tatttccaat ttgcaataat acgtttagct gttccctcac tgaaatggga
     8161 atagtgttat ggtcaaaacg ctgtcttccc aagaatagat ggattttagt agcatgtccc
     8221 aacaaggcaa tttgtgatag tagggagaaa aaagcttctt tgatattctt attgactccc
     8281 tctgatagag cgaggacttc atcaataaca atagttagat gggtaaattg atgattggga
     8341 ttatcataca agatagcttg tcgtttctga atgagagttg cacactggtt taattgttca
     8401 ttgacttgtg aaacgaaatc agattttgaa tggttttcta ctgggtggat aacggcaatt
     8461 ttattttctc ttgcccattg gctaggggta tcgaattttg ggtcaatgat aattaggccg
     8521 gacataggtt tcaggacact caagaagtaa gtgagagcgt acggtttccc agaacctgaa
     8581 tttccagcaa tagcccagtg actcccctta tctaaattga gttcgaagtg ctttatgaca
     8641 ggaattttcc cctgttgtaa gctagatgaa aatttatcta aatcaggaat cgccaagcgt
     8701 tctgaaatcc cctttctcca agggaagaat aatccacgtt gaatgtgtaa gtcatccgtc
     8761 tctaaaggga caaaatctgt gcaaattttg atttatctat tttttgactt ccgtttgact
     8821 taatcgataa agttgacctt gcttttttgg aaataactaa cgggacggac gtaaggtaag
     8881 attttgggat attttcttta tacattcgtt ctagaatatc atcaaccagg aatccgatat
     8941 taaatagtgg attttctttg agttttttta ttaggttagg gataatttct gtcgaaacat
     9001 tatcatacca ccatttattg atgaggggat caatgttttc ttgcctaaat gttgagggta
     9061 tgccatttcc gtctaattca aataggatac cacagacatt aacttcaaat gatttaatta
     9121 aattatcccc taacatcaac caaataatat caaacatttc ttcaaaatta aagacagtca
     9181 attcattgat ggtactggtt ctaaaacttc tagcgaggtt ataacttttt tcaccaacag
     9241 taatgtcaat gtcatctttc ttgtgaaaag tatccatttc agtttcatca gaagccaaaa
     9301 aattttttct ttgaatattg aaatttgcga atgtcttgga aagtctaaga cagctggatt
     9361 gtattgaaat taagtcatcg tccgaaaaag aatacattct tttatctaca gtttccaaat
     9421 ctctaaataa aatagagctg aagtagtggt aaaacatatt ttcaacaaac ttggcaatat
     9481 ctccaaaaat aatttcttta tagtccgagt cggtttgagt attcaaataa agcaatattg
     9541 aagcggtaac ttgtcgcttt cccttttcaa cttggctaac ttgacctgca ttacctaggt
     9601 cactttactt taaattatac ttttttctta aacatttgat acgagtaggt atttcttgtg
     9661 agtaattttc atcaaaaaat ttcatagata agccccctat aaacatggaa aagtggtaaa
     9721 aatagttgtt atcctagttg attaaaaaaa tctctgattt cctcatcttt tataaaataa
     9781 tatataattt tcccctctct tctagtgccc aagatgtttt gattggctag tttacgaaga
     9841 tggtgggagg cagatgccat actgagattt a
//
LOCUS       DAUCWU010000059         9675 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUCWU010000059 DAUCWU010000000
VERSION     DAUCWU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.059"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(109..765)
                     /locus_tag="VGY24_001294"
     CDS             complement(109..765)
                     /locus_tag="VGY24_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ4284272.1"
                     /translation="MEYKDIRDNLEEMMNDNYKDFIKALVSIEKGVNDEKALEDVYVL
                     YMNNNTTGLLSDDFDYMIDDMKEQGKIVENSNELEEKDDLINLVGNIAGKVENLEREN
                     ANGEKFKVSNFSIVSKDDDGNKIYTNCSAYGDKTKDLENLKQGDFVKIFGQVKTSIDN
                     NGKEHKNVRILSSKLLKAKEQVKSQDKDKKSILGQIKSFKTDDKAKSNKKDHSKGTER
                     "
     gene            complement(809..8452)
                     /locus_tag="VGY24_001295"
     CDS             complement(809..8452)
                     /locus_tag="VGY24_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ4284273.1"
                     /translation="MQVNDFKNIQEAIKYEVLQDEKEYLKLLKVIGNNQKYDFSSQLS
                     IYNKEPEARACATFDMWKKYFGRVVMRGEKGIPILVGSDINQRVSYIFDISQTTSMDR
                     NINEVSLWQFDHENHNEALKEIINNSSFEASDSLNENIFSLSRIYGDEYINLALADLR
                     IDIEDRLSFEKFMRDSISYAVANRFNTVYPMDMENLKNNFSRINTISLEQIGLVISRV
                     SEDIIDSTIEKSKEIDRARLLTERDAADYNKDIENINEDRGGQDDLYRRDDRSRSRDG
                     RVFTDGSDRGNSDEDRRESLGYDGEGSGIYGEISESDLRSPKTVLPSRERGHGELEET
                     SGNVRGENTFEPSEGNSRSGSGLYQERESQDDESTRIDREDDERESRGIPRTDEQLDG
                     NSEENGNQGIRGSLENEISQEKEADEASFFDGNNIEKRKDYWIVEFNENHELVPDYSG
                     QIVTKELINVLRQKDIDVKDHNQTLGENEFGEMTDDYIGYFKFYFDHYVDGEVVEHYR
                     IDLGDGEEVNEREFSYLEEQVALSEEKSSQEEVKENIELKFKIGDQVRYKDKDFTITD
                     FDELSGGLKTVTIRDNMEYMGGMIRGSEVIPYRNDSYLEEIFENLSQTSEKLAVKVGK
                     EFILEDENTFDGINLIETGTKVEVNGEEIPLYKGETFEESRKIDDLLDSGNYEIHKLS
                     EHEKQIERQVEQESFIDNYNPEIDQMMDRYNVPREAAENLLRGKEDLKNLGYEPNKER
                     LSFARNYDLKNHIYSEYLTPSEKLDKNIKAIKMLKRLENENRSPREYEQAYLADYLGW
                     GGLADVFDEEKGGQWLEARNILKENLTHEEYLNAKESTLTSFYTPREVMDGIYKTLTD
                     MGFKTGNILEPSAGVGNFIGNMPSEMKASKVYGVEKDSLSGRIARELYPEANIQIKGF
                     EETNFSNNFFDLVIGNVPFGDFKVNDREYNRNNFLIHDYFFAKSIDKVRNGGIIAFIT
                     SSGTMDKKDESVRKYINARCEFLGAMRLPNTTFKGLAGTEVTSDIIFLKKRDSVIERD
                     DDWIHLATDKKGLTYNKYFVDNPQMVLGDMKEVSGRFGNTITCDEKEDENLKDLMDLA
                     SKEISSNSKYEEVELLEDEELSLPATDDVKNFSYTIIDEEVYLRENSVLIKQNISDKN
                     KEKIKDYLDVMNALKDVIEKQKDDFSDEEIKESQAKLNEVYDNFSKKHGFINSLSNTR
                     ALKEDSNFPLVSSIEILDDEDNFKAKSDIFSKRTITKAKVVDHVDTSLEALVLSVSQK
                     GYVDFEYMESITSKDRNTLIGELEGEIFLDIKDTDLINNRMPFENFDNDDPFHFSYVS
                     ADEYLSGNIREKIGYLNSYIGEIENVIDLAPSEKKDTLLNELGKLKYQREKLQEVMPE
                     ELTASDINVRLGATWIPQKDIEDFTFNLLKTPGYDRWNINVRFSPHSSEWNIEGKSVD
                     STNDLANMTYGTSRVNAYKLIENALNLKDTKVFDQIINDDGSKTSVLNKKETMLASQK
                     QELIKEEFKNWIFEDPDRRYRLEKIYNEKFNSIRNREFDGSNLTFDGMNTEIRLREHQ
                     KNAIARTLYGGNTLLAHVVGAGKTFEMVASAMESKKLGLASKSLFVVPNHLTTQIGRE
                     FMQLYPSANIMVADKKDFQPKNRKRFIGRIATGEYDAVIIGHSQFEKISMSKEYQVRH
                     IQDQIDDIVSFIDENKRNRGENFTVKQLEKTKKKLLVRLEKLNDDFKKDDVITFEELG
                     VDKLFIDEAHNYKNLFLHTKMRNVAGIGQSEAFKSSDMYMKCRYMDEMTDGKGVVFAT
                     GTPISNSMTELYTMQRYLQYDDLKARGLEHFDAWASTFGETENTFELSPEGTGYRQKT
                     RFSKFYNLPELMSMFKEVADIKTSDMLNLPVPEANFEVIKTKPTEEQKEILEAISERA
                     DAVRNNQVEPTEDNMLKITNDGKKLALDQRLINPLLPDDPNSKVNVCVKNIFSIWDKT
                     KENSSTQLVFSDMSTPKGDGEFNIYDDIRNKLVNMGIPKEEIAFIHEADTDKQKDELF
                     SKVRRGEVRVLLGSTQKMGAGTNVQNKLIALHDLDVPWRPSDLEQRSGRIVRQGNEND
                     KVNIFRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDEASLSYSEIKALA
                     TGNPIIKEKMDLDNEVTKLKMLEANYKSNKYKLEDKVNKIYPQSILKTEMEIQAVKED
                     IASVEKLGEGDSKFTSISLGENKILDKKEAGEKLLEEIKKVKINDSKVIGKYRNLDLQ
                     VSYNFMTNTHTFKLLGKAEYFGEFSNSTDGNITRLDNAIEKMPSRLERLNQNLENYKE
                     SLENAKVELTKPFEKADELRDKTLRLAEINKLLDMGEVEELENQSPLLEDLKRAIVDY
                     SNYEFSESNSYEDFDKLYPDLSHIGLAYTETPDGKHSIQYEVNLEEKTWTQYVDNVAI
                     RTESFVEDGISNSQAIKDMTEAIKMSSFDDLVSVDEEDVKQALGLEIDDDGNFYDPLA
                     KDLDNDGIPDRYDNDFKDSDYFESTYDVEDNLHAREEKPSILGQISKFKSEEEKDKNQ
                     EKSEKGQER"
     gene            complement(8439..9404)
                     /gene="dcm"
                     /locus_tag="VGY24_001296"
     CDS             complement(8439..9404)
                     /gene="dcm"
                     /locus_tag="VGY24_001296"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ4284274.1"
                     /translation="MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDKACVKSYN
                     ELYEENYKPKSVLGYKAPNEKIDLIMHGSPCQDFSRIGKKQGGVKNSGTSSSLLFETI
                     RIIKEMKDKPKWIIWENVKGVLDRNMRDSFFIYLKELEDLGYESKYEILNAMDFGIPQ
                     KRERIFVVSCLGANNFSFNKLERKETRPLSEFLEKDVSELYTMTQPYMLKFLNKGIDN
                     SFKGRLKVIKDFSYTISTKQMRVPNSGIIDIGNGQYRYLTERECLRLMGFDDSDIDKL
                     EKVHPRRKNCTSSKLYKQAGNSIVVDVLMAIIKEIHRMEVGNASK"
     gene            complement(9397..>9675)
                     /locus_tag="VGY24_001297"
     CDS             complement(9397..>9675)
                     /locus_tag="VGY24_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEQ4284275.1"
                     /translation="GLGTPATRAGIIENLIHKDLIRRDKKNLLVSEKGNRLVSIVEDK
                     FKSAETTSEWEMKLAKISSGEVDKEDFLREIEDSIKELVDRYKNNLNE"
BASE COUNT         2623 a         1929 c         1184 g         3939 t
ORIGIN      
        1 ttattgcata ctaattaatt ataacattaa ctataggtta ttttcaatca tttgatatat
       61 cactttaaat gaacaaaaaa ataatggcag tccgaagact gccgatattt atctctctgt
      121 tcctttgcta tggtctttct tgtttgactt agctttgtca tctgtcttaa agctttttat
      181 ttgccctaat atggactttt tatctttatc ttgactcttt acttgttctt ttgcttttaa
      241 aagcttagaa gacaaaatac ggacattttt atgttccttt ccgttgttgt caatacttgt
      301 ttttacttgt ccgaagattt taacaaaatc tccttgttta aggttctcta aatcttttgt
      361 cttatctcca taggctgaac aattggtata aattttattt ccatcgtcat cttttgaaac
      421 aattgaaaaa ttacttactt taaacttttc tccattagca ttttctcttt caagattttc
      481 tactttgcca gctatattac ccacgagatt tatgaggtcg tctttttctt caagttcgtt
      541 ggaattttca acaatcttac cttgttcttt catatcatca atcatatagt caaagtcatc
      601 acttagtaaa cctgttgtgt tattgttcat atataaaaca tagacatctt caagtgcctt
      661 ttcatcatta acgccttttt ctatgcttac aagtgctttt ataaaatcct tgtaattatc
      721 attcatcatt tcttctaagt tatctctaat atctttgtat tccataattt cctccttgta
      781 ctaaataagg agagccttac gctctccttt atcgttcttg tcctttttca cttttttctt
      841 ggtttttatc tttttcttct tctgatttga attttgatat ttgtcccaat attgatggtt
      901 tctcttccct tgcgtgtaga ttatcctcta catcataagt tgattcaaag taatcactat
      961 ctttaaaatc attgtcatac ctatctggta ttccgtcatt atcaagatct tttgctagtg
     1021 gatcatagaa gtttccgtca tcatctattt ctagtcctag tgcttgtttc acatcttctt
     1081 catctactga taccagatca tcaaaacttg acatctttat ggcttcagtc atatctttaa
     1141 ttgcttgtga gttagatata ccgtcttcta caaaagattc tgttctaata gctacattat
     1201 ctacatactg agtccatgtt ttttcttcca aatttacctc atattgaatc gagtgcttac
     1261 catctggtgt ttctgtatag gcaagtccta tatgacttaa atcagggtat aatttgtcga
     1321 agtcttcata gctattagat tctgaaaact cgtaattaga ataatcaact atcgccctct
     1381 ttaaatcttc taataatggt gattggtttt ccagttcttc cacttctccc atatctaaaa
     1441 gtttattaat ctcagctagt cttagagtct tatcccttaa ctcatctgct ttttcaaatg
     1501 gtttggtcaa ttctactttg gcattttcta aagattcttt atagttttca aggttttgat
     1561 ttaacctttc tagtcttgaa ggcatttttt caatggcatt atcaagcctt gttatattac
     1621 catcagtaga gtttgaaaac tctccaaagt attctgcttt tcctagaagt ttaaaggtgt
     1681 gagtattagt cataaagtta tatgaaactt gtaagtctaa atttctatat ttaccaatga
     1741 ccttactatc atttatcttt acctttttta tttcttctaa tagcttctct ccggcttctt
     1801 tcttatctaa aattttattt tctccaagac tgattgaagt gaatttgcta tctccttctc
     1861 ctaatttctc aacacttgca atatcttctt taactgcttg tatttccatt tcagttttta
     1921 aaatactttg aggataaatt ttatttacct tatcttcaag cttgtattta ttagacttgt
     1981 agtttgcttc cagcattttt agctttgtaa cttcgttatc taaatccatt ttttctttaa
     2041 ttataggatt gcctgtagcc aaagccttaa tttctgaata agatagactt gcttcatcaa
     2101 catcttctgc aacacgcaca ggtgtcttgc ttgtcataat ttgggatata aatttctgct
     2161 tattttctat tgtctgccac aaataagcat caaaggtatt ctctgttaca tatctaaaga
     2221 tatttacctt atcattttca ttgccttgac gaacaattct accacttctt tgctctaggt
     2281 cagacggtct ccaagggaca tctaaatcat gtaaggctat taatttattt tgtacatttg
     2341 tacctgctcc cattttttga gtagagccta ataatactct tacttctcct cttcttacct
     2401 ttgagaataa ttcatctttt tgtttgtctg tatcagcttc gtgaataaag gctatttctt
     2461 cttttggtat tcccatattc actagcttat ttctaatatc atcatagata ttaaattctc
     2521 catctccttt tggtgtagac atatctgaaa atactaattg tgtcgatgaa ttttcttttg
     2581 tcttatccca gatcgaaaag atatttttta cacatacatt tacctttgaa ttaggatcat
     2641 cgggtagtag tggatttatt aatctttggt caagggcaag ttttttacca tcatttgtaa
     2701 tctttaacat attatcttct gttggctcga cttggttatt tctaactgcg tcagctcttt
     2761 ctgaaatagc ttctaatatt tctttttgtt cttcagtagg ttttgtttta ataacttcaa
     2821 aatttgcttc aggaactggc aaatttaaca tatctgaagt ctttatatct gctacttctt
     2881 taaacataga catcaactct ggcaagttat aaaacttcga aaatcttgtc ttttgcctat
     2941 atccagtgcc ttctggagat aattcaaagg tgttttctgt ttctccaaaa gtagaagccc
     3001 aagcgtcaaa atgttctaat cctcttgcct ttaaatcgtc atattgaaga tatctttgca
     3061 ttgtataaag ctctgtcatt gaatttgata tcggtgtacc agtagcaaat acaactccct
     3121 ttccatctgt catttcatcc atatacctac atttcatata catatctgaa gacttaaagg
     3181 cttcactttg accgataccc gcaacatttc tcattttagt atgcaagaat aagtttttgt
     3241 aattatgagc ttcgtctata aataacttat ctactcctag ttcttcaaag gttattacat
     3301 catctttttt aaagtcatcg tttagctttt caagtcttac caagagtttc ttctttgtct
     3361 tttccagttg ttttacagtg aaattttctc ctctatttct tttgttctca tcaataaagg
     3421 aaactatgtc atctatttga tcttgtatat gccttacctg atactcctta gacattgata
     3481 ttttttcaaa ttgagagtgt cctatgataa ctgcatcata ttctcccgtt gcaatcctac
     3541 caataaatct tttcctattt tttggttgaa agtctttttt atcggccacc ataatatttg
     3601 ctgacggata taactgcata aactctctac caatttgagt ggttagatgg ttaggaacta
     3661 caaataatga tttacttgca agtcctaact ttttagattc catagcagaa gctaccattt
     3721 caaaagtttt tcctgctcct actacatggg caagtagtgt atttccacca taaagagtcc
     3781 tagctattgc atttttttga tgttctcgaa gtctaatttc agtattcatt ccatcaaaag
     3841 ttaagttaga accatcaaat tctctatttc taattgaatt gaatttttca ttataaatct
     3901 tctctagcct atatcttcta tcaggatctt caaatatcca attcttaaat tcttctttta
     3961 tcagttcttg cttttgactt gctagcatag tttctttttt gtttaataca gaagtcttag
     4021 acccatcatc atttattatt tggtcaaata cttttgtatc ttttagatta agagcatttt
     4081 caattaactt ataggcattt actctacttg taccataagt catgttagca aggtcattag
     4141 tcgagtcaac acttttaccc tcaatattcc attcacttga gtgtggcgag aacctaacat
     4201 ttatattcca cctatcataa cctggtgttt ttaaaaggtt aaaagtaaag tcttctatat
     4261 ctttttgagg tatccaggtt gctcctaacc ttacatttat atcagaagca gtaagttctt
     4321 caggcataac ttcttgtaat ttttctcttt gatatttgag tttgcctaac tcgtttaata
     4381 gtgtatcttt cttttcagaa ggtgctaaat ctattacatt ttcaatttct ccgatatatg
     4441 aattgaggta gccaatcttt tctctgatat taccacttaa atattcatca gccgatacat
     4501 atgaaaaatg aaatggatcg tcattgtcaa agttctcaaa gggcattctg ttatttatta
     4561 ggtctgtatc cttaatatct aaaaatatct cgccctcaag ctcaccaatt aaggtgttcc
     4621 tatctttact tgtgatagat tccatatatt caaaatctac atatcctttt tgtgaaactg
     4681 ataagactaa agcttcaagg gaagtgtcta catggtctac tacttttgcc tttgtgattg
     4741 ttctttttga gaatatatca ctcttagctt tgaaattatc ctcatcatca agtatttcta
     4801 ttgatgatac caaaggaaag tttgagtctt cctttaaggc tctagtgttt gataaggagt
     4861 taataaagcc atgtttcttt gaaaagttat catagacttc atttaacttt gcttgtgatt
     4921 cttttatttc ctcgtcagaa aaatcgtcct tttgtttttc tattacatct tttaaggcat
     4981 tcataacatc aagataatct tttatctttt ctttgttttt atctgatata ttctgcttaa
     5041 ttaagactga gttttctctt aggtagacct cttcatcaat aatggtgtag gaaaagttct
     5101 taacatcatc tgtcgctggt agacttaatt cttcatcttc caatagttcg acctcttcat
     5161 attttgaatt tgaagatatt tccttacttg caagatccat taaatccttt agattttcgt
     5221 cttctttttc atcacaagta attgtatttc caaatcttcc agaaacttct ttcatatccc
     5281 ctaataccat ttgaggatta tctacaaaat atttgttgta agtcaaaccc tttttatcag
     5341 ttgctaggtg tatccaatca tcatctcttt ctataactga atctctcttc tttaagaaaa
     5401 taatatctga agtaacttct gtaccagcaa gtcctttaaa tgttgtatta ggaagcctca
     5461 tagctcctaa aaactcacat cttgcattaa tatattttct aacagattca tcttttttat
     5521 ccatagttcc agatgatgtt atgaaggcaa taattcctcc attcctaacc ttatctattg
     5581 actttgcaaa aaaataatcg tgaatcagaa agttatttct gttatattca cgatcgttaa
     5641 ctttaaaatc tccaaaggga acatttccta ttaccaagtc aaagaaatta tttgagaaat
     5701 ttgtttcttc aaagccttta atctgaatat tagcttcagg ataaagttct cttgctattc
     5761 ttccgcttag gctgtctttt tctactccat aaactttaga cgctttcatt tcacttggca
     5821 tattaccaat gaaattaccg actcctgcag atggttcaag gatatttcca gtcttaaatc
     5881 ccatatctgt tagagtctta tatattccat ccattacctc tctaggtgta taaaacgatg
     5941 ttaaggtaga ctctttagca ttcaaatact cttcatgtgt taaattttct tttaaaatgt
     6001 ttcttgcttc aagccattgt cctccctttt cttcatcaaa tacatcggca agacctcccc
     6061 aacctagata atcagcaaga taggcttgtt catattctct tggactccta ttttcatttt
     6121 caagtctttt tagcatttta atagctttga tatttttatc tagcttttct gatggtgtaa
     6181 ggtattctga gtagatatga tttttcaagt cataatttct agcaaaactt agcctttcct
     6241 tattaggttc atagcctaga ttctttaaat cttcttttcc cctcaataag ttttcggcag
     6301 cttccctagg cacattgtat ctatccatca tttggtcaat ttctggattg tagttatcaa
     6361 taaagctttc ttgttctact tgtctttcga tttgtttttc atgctcagat aatttgtgta
     6421 tctcatagtt tccactatct aaaaggtcgt caatctttct actttcttca aatgtttcac
     6481 ctttatataa ggggatttct tctccattaa cttctacctt agttcctgtt tcaattaagt
     6541 taattccatc aaaggtattc tcatcttcta aaataaattc cttaccaacc tttactgcta
     6601 gtttttctga tgtttgactt agattttcaa agatttcttc aaggtatgaa tcgtttctat
     6661 atggaattac ttccgaaccc ctaatcatac ctcccatata ttccatatta tctctaatgg
     6721 ttacagtttt aagtcctcca cttagttcat caaaatctgt aatggtaaag tccttatctt
     6781 tatatctaac ctgatcgcct atcttaaatt taagctctat attttcctta acttcttctt
     6841 gtgaagattt ttcctcactt agtgcaactt gttcttctaa atatgaaaat tcacgctcat
     6901 ttacttcttc accatcacca aggtcaatcc tatagtgttc gacaacttct ccgtctacat
     6961 agtggtcaaa atagaatttg aaatatccta tataatcatc tgtcatttct ccaaattcat
     7021 tttctccaag tgtttgatta tggtctttaa catcaatatc tttttgtctt aggacattaa
     7081 ttaattcttt ggttactatc tgcccactat aatcgggaac taattcgtgg ttttcattaa
     7141 attctacaat ccagtagtct tttctttttt caatattgtt gccatcaaaa aaagaagctt
     7201 catcagcttc cttttcttgg cttatttcat tttctaagct tccacgtatt ccttgattgc
     7261 cattctcttc actgttgcca tcaagttgtt catctgtcct tggtattcct ctggattctc
     7321 tctcatcatc ttctctgtca atccttgtgc tctcatcatc ttgactttct ctctcttgat
     7381 aaagtccact gcctgatctt gaatttcctt cagatggttc aaaagtgttt tctcctcgta
     7441 catttcctga agtttcttcc agttctccat gtcctcgctc cctactaggt aagacagtct
     7501 taggactgcg taagtcggat tcggaaatct ctccataaat tccagatcct tctccatcat
     7561 atccaaggct ttctcttcga tcttcatcgc tatttcctct gtcgcttcca tctgtgaaaa
     7621 ctcgtccatc tctacttctt gacctatcat ctcgtctata taaatcatct tgacctcctc
     7681 tatcttcgtt tatattttct atatctttat tatagtcggc agcgtccctt tctgtcagca
     7741 gcctagcacg atctatttcc ttagattttt ctattgtgct atctataata tcttcactaa
     7801 cccttgatat tacaagacct atctgctcta aagaaattgt attaatccta gaaaaattat
     7861 tttttaaatt ttccatatcc atagggtaga ctgtattaaa cctattagct actgcatagg
     7921 atattgagtc cctcataaac ttttcaaagc taagtctatc ctctatatct attctcaaat
     7981 cagcaagagc caagttaatg tattcatctc cataaattcg gcttaaagaa aatatatttt
     8041 cattaagtga atcactagcc tcaaatgaag aattatttat tatttctttt aaagcctcgt
     8101 tatggttttc gtgatcaaac tgccataagc taacctcatt aatattccta tccattgatg
     8161 tcgtctgact aatatcaaag atgtatgaaa ctctttgatt tatatcactt ccaactaaaa
     8221 ttggaatacc cttctctcct ctcataacaa ctcgaccaaa atatttcttc cacatatcaa
     8281 aagtcgcaca agctctagct tcaggttctt tgttatatat acttaattgg ctagaaaaat
     8341 catatttctg attattgcct ataactttta agagctttaa atattctttt tcatcttgta
     8401 aaacctcata ctttattgct tcttgtatat tcttaaaatc atttacttgc atttcctacc
     8461 tccattctat gaatttcttt tataatagcc attaaaacat caaccacaat agaattgcca
     8521 gcctgcttat atagtttgct tgaggtgcaa ttttttcttc ttggatgtac tttctctaat
     8581 ttgtctatat cactgtcatc aaaacccatg agtcttaaac attctctttc tgttaaatac
     8641 ctatactgac catttccaat atctattatc ccagaattgg gtactctcat ctgttttgta
     8701 gaaatagtat aagaaaaatc tttaatcact tttagtcgcc ctttaaaact attatctatg
     8761 cctttattta aaaatttgag catataaggt tgggtcattg tgtaaagttc acttacatcc
     8821 ttttctaaaa attcacttag tggtcttgtt tcttttctct ccaatttatt aaaagaaaag
     8881 ttatttgctc caagacatga aacaacaaat atcctttctc ttttttgagg tatcccaaag
     8941 tccattgcat ttaagatttc atatttgctt tcatatccaa gatcttctag ctcttttaga
     9001 taaataaaaa aggagtccct catatttcta tcaaggactc cctttacatt ctcccaaatt
     9061 atccatttag gtttatcttt catttcttta attattctaa ttgtttcaaa taataagctt
     9121 gaactagttc ctgaattctt tactcctccc tgcttttttc ctattcttga aaagtcttgg
     9181 caaggacttc catgcattat taagtctatc ttctcattag gtgctttata ccctaatact
     9241 gattttggct tataattttc ttcataaagt tcgttgtatg atttaacaca agccttatct
     9301 atctctacat aatcaacaac ttcataaggt atctttaaat taataaaagc ctttctaata
     9361 gcacctatgc caccgaaaag ctctaatatt tttaccttat tcatttagat tattcttgta
     9421 cctgtctaca agctccttta tactatcttc aatttctctt aaaaagtctt ctttatctac
     9481 ttcgccagag ctaatctttg caagtttcat ttcccattca gatgttgttt ctgccgactt
     9541 aaacttatcc tctacaatcg atacaagtct atttcctttt tctgatacaa gtaaattttt
     9601 cttatctctt cttataagat ccttatggat aagattttcg ataattcctg ctcttgtagc
     9661 tggtgttcct aagcc
//
LOCUS       DAUCWU010000060         9597 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUCWU010000060 DAUCWU010000000
VERSION     DAUCWU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.060"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..480)
                     /gene="coaC"
                     /locus_tag="VGY24_001298"
     CDS             complement(<1..480)
                     /gene="coaC"
                     /locus_tag="VGY24_001298"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ4284276.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     "
     gene            complement(473..1165)
                     /gene="coaB"
                     /locus_tag="VGY24_001299"
     CDS             complement(473..1165)
                     /gene="coaB"
                     /locus_tag="VGY24_001299"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ4284277.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNEHLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNSNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            1346..2197
                     /locus_tag="VGY24_001300"
     CDS             1346..2197
                     /locus_tag="VGY24_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ4284278.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            2194..3393
                     /locus_tag="VGY24_001301"
     CDS             2194..3393
                     /locus_tag="VGY24_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ4284279.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            3390..4388
                     /locus_tag="VGY24_001302"
     CDS             3390..4388
                     /locus_tag="VGY24_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ4284280.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMSLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEKAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            4430..4762
                     /locus_tag="VGY24_001303"
     CDS             4430..4762
                     /locus_tag="VGY24_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ4284281.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            4755..5567
                     /locus_tag="VGY24_001304"
     CDS             4755..5567
                     /locus_tag="VGY24_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ4284282.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            5560..6441
                     /locus_tag="VGY24_001305"
     CDS             5560..6441
                     /locus_tag="VGY24_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ4284283.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILGTKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            6488..7507
                     /locus_tag="VGY24_001306"
     CDS             6488..7507
                     /locus_tag="VGY24_001306"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ4284284.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            7635..9305
                     /locus_tag="VGY24_001307"
     CDS             7635..9305
                     /locus_tag="VGY24_001307"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ4284285.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKLDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            9390..>9597
                     /locus_tag="VGY24_001308"
     CDS             9390..>9597
                     /locus_tag="VGY24_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ4284286.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVL"
BASE COUNT         2896 a         1714 c         2060 g         2927 t
ORIGIN      
        1 tttatctcca caggctaata agctagattt tggaggaatt tctgtaaacc ctattgtcga
       61 taagcgttta atattttctt gggtaatagg gttttgatac attttggtat tcatggctgg
      121 ggctattaat tttggtgtgg tagctggtag ggcaagagca acacttgtta caatattatc
      181 agcgaatcca tatgctagat gagcaatagt attagcagag gcaggtgcaa caataaataa
      241 atctgtgcgt tttgctaatt cgatatggtt gataactttt gggtcatgct catccataac
      301 atctaaatga atagcatttt ttgataacac ttgcagggtt aatggtgtaa taaattgtgt
      361 agcagcctga gtcattatga tgtgaacatc atagccaatt ttagttaatt gactcgtaag
      421 atctgctgct ttataggcag atatactacc agaaacagct aaggtaatat gtttagtcat
      481 gtttggtcat cctttcatat aataagtcag caattgcctc tttggtatct gcagatgcaa
      541 cttcgttatt ggatatcaaa agagccttgt gcatacctgt ttggatatca actaaatcat
      601 tggcgagaat ataatcagca tggtttttag ctagacttgc tctggctact ttaattaact
      661 cttcttgagg cacattaact agaagtttaa agccgactag tttaatatta gaattccact
      721 gtttaacata agagataact ttgggtgttt ttttcaaaaa aagaacctga taatcagatg
      781 cagatgagat tttcggctca ctgttatgtt cacatagaaa acaattaagg ttatcagctt
      841 gagagacttg ttccaaatca gtcatgtaga ctggagtata gtcagaaaca gccatactat
      901 gaattagaat gtcatggtga ggaacctgat ctttgagtgc agccattaag tcgttaactg
      961 tttccacttc aataatcctt aggtgctcat ttggcagagg tttagtggct gttttagtag
     1021 tgactagggt aacgtcatga tgatattgta aaaatcgctc tgtgattaat tttccaagtt
     1081 gcccagttga gtgattagtg atacctcgta ctgcatctat aggttcagtt gttccacctg
     1141 atgttattat taatttcata gtcatatttt atcaaaaaag gagctgatag taaacggaag
     1201 ataatgacgc cagacattgt cttgcgtttg tattaatatg gaatagtaat agatttctag
     1261 taattaaaac gaagttctgg aaagcaagac actatagtag ctaaaatcta aattttaagg
     1321 taaaatagag caagcggttt caagtgtgaa aggaagaatt gtgataacta ttagagattt
     1381 agcgtcactg ccgatttttg tttacggtga tggaaataaa aaagttccag gagctttgag
     1441 tccttttgtg tggacagagg tgtttttaaa aactatcaat caagaaccta atcaactgat
     1501 cttacatatt tggccgatga caagaacagt tatattagga atgttagata gacagcttcc
     1561 ctactttgag ctggctaaaa cagagattgg caataatggt tatgttcctg ttactcgtaa
     1621 tattggcggt ttggctgttg tcgctgatga tgggattttg aatttttcgc ttgttattcc
     1681 agaccatttt tcggaatcta tcagtattag taatgcttat ttaataatgg ttgatgtcat
     1741 tagagagagt ttttcggatt attatcagcg tattgaatat cacgaaatta aaaattctta
     1801 ttgccctggt aattttgatc ttagtattgc aggccgcaag tttgcaggta tcgcccagcg
     1861 tcgtatcaaa aagggaattg ttgtgtctat ttacttgagc gtttgtgggg atcaagcagc
     1921 gcgtggtcaa ctcatcaagg atttttatga ggccggaaca caaggagaag ttaccaaggt
     1981 taattaccct caaattgatc cagaatgcat ggcaactctt tcagaactcc ttgagacgcc
     2041 ttttacagtg gcagaagtcc ttgaacgtct tcgattgacc ttgcgacagc ttggcttttc
     2101 cctcacggaa aaaagtccag atcaagcctt actgactaat tttgatgctg tctatgaacg
     2161 gatgcagcta gaagtcgtca gaaaggaagg caaatgaaag aaagatttag tcctttattt
     2221 gagcctttga ctttgccaaa tgggagtcaa ttggataatc gctttgtttt gtctcctatg
     2281 gtgaccaact catccacaaa agacggttat gtcacccaag atgatgtatc ctatgctttg
     2341 agacgagcag cttctgcccc tctacaaatt acaggagcgg cctatgtgga tccttatggt
     2401 caattgtttg aatatggttt tagtgttaca aaagatgccg atatttctgg cttgaaagag
     2461 ctagctcaag cgatgaaggc aaagggagct aaagctgtct tacaactgac ccacgctggc
     2521 cggtttgcta gccatgcctt gactaaatat ggctttgtct atgggcctag ttacatgcag
     2581 ttgagatctc cccaacctca tgaagtcaaa cctttaacag gtcagcaaat agaggaattg
     2641 attgcagctt acgctcaggc cactcggcgg gcaattcaag caggctttga tggcgtggaa
     2701 gtatcttcgg cgcagcgctt attgattcaa acgttctttt cgactttttc gaacaagagg
     2761 acagatagtt atggttgtca aacacttttt aatcgctcta agttaactct agctgttttg
     2821 caagctgttc aacaggtgat taaacaagaa gctcctgatg ggtttatttt tggatttcgt
     2881 gctacaccag aagaaactag gggaaatgat attggttata gtattgacga gtttttgcag
     2941 ttgatggatt gggtacttaa tgttgcaaaa cttgattatt tagcgatagc aagttggggc
     3001 cgtcatgtat ttcgaaatac tgttcgttct cctggccctt attatggaag gcgagttaat
     3061 caggtagtta gagattattt aagaaataag ctacctgtta tggctacagg gggaatgaac
     3121 acacctgaca aagctattga agcactagct catgctgatt ttattggagt atctacacct
     3181 tttgtcgtgg atccagaatt tgctcataaa atcaaagaag ggtgtgaaga gagtattcat
     3241 ttaaggattc gacctgctga tttgaaaagt ttagcgattc ctcaggcttc tttcaaagat
     3301 attgtacctt tgatggacta cggggaatca ttgcctaaag aatcaagaac tttatttcgt
     3361 agtcttactc acaattataa ggagattaaa tgaaagtcag tattcttgat tatggggtga
     3421 ttgataaaga aaaaacaccc caagaagctt tattagaaac gcgctgctta gcacaagtag
     3481 ccgacaaact tggttttcat cgtttttggg tagcagagca tcataatatt tatgcctttg
     3541 caataagcag tccagagctg ttgatgatgc atttagcaga tcataccaaa cagattcgga
     3601 taggttcagg aggtattatg tctctacatt atagtagctt taagattgca gaatggataa
     3661 tgactttaga ggccttgcac cctaatcgga ttgatttagg tattggcaac tctttaggaa
     3721 ctacgttagt tcaaagagct ctatcaagta tccattgcaa agacagctat agtcaggttg
     3781 tcacagaatt ataccagtat cttaatcctg atcatttgtc accactaccg atttttgtta
     3841 accctagggg taatacttac cctcagattt ggacgctaag caatagtttg gagacagcag
     3901 aactggctgg tcaattaggt ttaggctata cctttggtat tttcccctat ataccaaaag
     3961 atcctataac agaagctaaa agagtatcgg cacattatag aaaggctttt aggccttcaa
     4021 agctattaaa aatccccaag ttaatattag cagtttttat tgtcctttct gataccgacg
     4081 agaaagcaga agctttggct aaaccgttgg acatttggat gcttggtcag caagatttta
     4141 atgaatttaa gacctaccct gatgttgagg aggctagaaa ctatcactta acagaaaaac
     4201 aaagagaagc cattgcagca aatcgtagtc gcatggttat tggatcacct cacacagtca
     4261 agaaacaatt ggataggctg atagaagctt gtcaagctga cgaattatta gccatcccct
     4321 tagttcctga atttgccaat agacaacgaa ccttagagct gctagcagac ttatacgtga
     4381 ccatttaacc ttaccaaaaa caattcccta aactgaaagg aaagaaacaa tgaaaaaaat
     4441 tgcaaattac ttattaattg agaaaacaga tgaccgctat accattagca tgacaccaga
     4501 attacaagac gatattggta ctattggtta cgctgaattt acggataacg accaccttgc
     4561 cgttgatgac attatcttaa atctagaggc ttctaagacc gtcatgtctg tcttgtcacc
     4621 attggctggg gcagttgttg agcgcaatga ggcagccaca ctaacaccaa cccttttgaa
     4681 ttcagaaaaa gctgaggaaa attggattgt tgtgctaaca gatgttgatc aagcagcttt
     4741 tgatgcccta gaagatgcct agttcatttg atttattagg ggaaatgatt ggtctgttgc
     4801 aaacagagca gcttactagt tcttgggcct gtcccttgcc taatgcttta acaaagcgac
     4861 aagacttgtg gcgggcgctc attaaccagc gccctgcttt gcccttatca aaagattact
     4921 tgaatctgga agatgcttat ctagatgatt ggcgtgcttc ttttgttcct gtatcagtca
     4981 aggattgcca aaaaactaat tataccagtc tttttcttta ccatggggat attaggtacc
     5041 ttgctgtgga tgctattgtc aacgctgcta atagtgagtt attgggatgt tttattccta
     5101 accatggctg tattgataat gcgattcata cgtttgccgg aagtcgttta cgcttagctt
     5161 gtcaggcaat catgactgaa caaggccgta aggaagccat tggacaagca aagcttacca
     5221 gtgcttatca tctaccagcc tcttatatta ttcataccgt agggccaaga attaccaaag
     5281 gacaccacgt ttctccgatt cgagctgatt tgttagcacg ttgttatcgt tctagtcttg
     5341 atttggcggt taaagcagga ttgactagcc tagctttttg ctctatttca acaggagaat
     5401 ttggctttcc taaaaaagaa gccgctcaga ttgctattaa gacagtttta aaatggcaag
     5461 cagaacatcc agaatctaag accttaacta tcatttttaa tacctttaca tcagaagaca
     5521 aggccttata cgacacttat ttgcaaaagg agaataactg tgagtaattg gacaacctat
     5581 cctcagaaaa acctcaccca ggcagaacaa ctggcccaat taatcaaaga agcagatgca
     5641 cttgtagttg gtattggagc tggcatgtct gcagctgatg gttttactta tatcgggccg
     5701 cgttttgaga cggcattccc agattttatt gctaagtatc agtttttgga tatgctgcaa
     5761 gctagccttt ttgattttga agattggcaa gaatattggg cttttcaaag tcgctttgtc
     5821 gctttgaatt atcttgatca gcccgtaggc cagtcttatc ttgatttaaa agagattttg
     5881 ggaaccaaag actatcatat tattactact aatgctgata atgctttttg ggtggctggt
     5941 tatgatcctc ataacatttt tcacattcaa ggagaatatg gtttgtggca atgtagccag
     6001 cactgccatc agcaaactta taaggacgat acggtcatcc gtcaaatgat cgcagagcaa
     6061 aaaaacatga aagtcccagg tcaactgatt cctcattgcc ctgaatgtga ggcgcctttt
     6121 gaaatcaata agcgtaatga agaaaaagga atggttgaag atgctgactt tcatgcccaa
     6181 aaagcacgtt atgaggcttt tttatcagaa cataaagagg gtaaagtgct ttatctagaa
     6241 attggtgtgg gacatacgac gcctcagttt atcaaacacc ccttttggaa acgtgtctca
     6301 gaaaacccta atgccttatt cgttaccttg aatcacaaac attaccgcat ccctctgtca
     6361 ataaggcgac aaagcctaga actgacagaa cacattgcac aattgattag cgccacaaaa
     6421 accatttacc aaaaatcgta accaaagcca atagacggtt aattaagaga tagagaagga
     6481 gactaacatg taccttattg aaccgattcg taacggtaaa cgtatcaccg atggagctgt
     6541 tgctctagcc atgcaagttt atgttcaaga aaatctattt ttagatgatg atattttatt
     6601 tccctactat tgtgatccca aagttgaaat tggaaaattc cagaatgcag ttgtagaaac
     6661 taatcaagag tatttaaaag agcatcatat ccctgttgtt cgcagagata ctggcggtgg
     6721 tgccgtttat gtagattctg gtgctgttaa tatttgctac ttgattaatg ataatggtat
     6781 ttttggtgat tttaaaagaa cctatcagcc cgctattgaa gcccttcatc atttgggagc
     6841 aacagaggtt gaaatgtctg gacggaatga cttggttatt gatgggaaaa aggtatctgg
     6901 cgctgcgatg actattgcta atggccgtgt ttatggggga tattcccttt tgctagatgt
     6961 ggactttgaa gccatggaaa aagcattaaa acctaatcgc aaaaaaattg aatcaaaagg
     7021 catcaggtca gttcgtagtc gcgttggaaa catcagagaa catttagcac cacaatacca
     7081 aggtattacg attgaagagt tcaaagattt aatggtttgc caattgttac agattgagac
     7141 gattagccaa gctaagcgtt atgatttaac ggaaaaagac tggcaacaaa ttgatgcttt
     7201 aacagagcgt aaatatcaca attgggaatg gaattatggc aacgcgcctc aataccgtta
     7261 ccatagagat ggccgtttta ccggtggaac agtagatatt catttagata ttaaaaaagg
     7321 ctatattgca gcttgtcgca tttacggaga tttttttggc aaggcagaca ttgcagaact
     7381 tgaaggtcac ttgataggaa cacgaatgga aaaagaggat gtcttagcta ccctcaatgc
     7441 aattgattta gccccttatt taggtgcgat tacagcagaa gaattaggag atttgatatt
     7501 ttcttgataa aaaagatacc tcagaccggt aaagcgcgat taaaaacgaa caataattgt
     7561 tttaattcaa ataaatgtac gtatggtata gattgtgata taatgacgtc attacgtaaa
     7621 attgaggagt catcatgaaa tcagatatcg aaattgccca aagtgtcgcc ttacaaccaa
     7681 tcactgacat tgttaaaaag gttggaatag acggcgatga cattgagctt tatggcaaat
     7741 ataaggctaa attgtctttt gagaaaatga aagctgtcga agctaatgag cctggaaaat
     7801 taatcttggt aactgctatt aaccctactc cagcaggaga aggaaaatca accatgtcta
     7861 ttggactggc agatgcctta aatcaaatgg gaaaaaaaac catgcttgcc ttacgtgaac
     7921 cctcccttgg tcctgttatg gggattaaag gcggggcagc aggtggtggt tatgcccaag
     7981 tattgcctat ggaagacatt aatcttcact ttactggtga catgcatgcc attacaaccg
     8041 ctaataatgc attatctgct ttgattgaca atcatttgca gcaaggaaac gatttaggaa
     8101 ttgatcctcg ccgcattatt tggaagcgcg tgttggacct taatgatcgc gctcttcgtc
     8161 aggtaattgt tggcttaggt agtccagtga atggtgtgcc acgcgaagat gggtttgata
     8221 tcacagtagc ttctgaaatc atggcaattt tatgtttggc aactgactta aaagatctaa
     8281 agaaacgtct tgctgatatc gtcgtggctt atacctatga tcgtaagcct gtctatgtgc
     8341 gtgacttgaa ggttgaagga gcattgacat taattctaaa agatgctatc aagccaaact
     8401 tggtgcagac tatttatgga acaccagctc ttattcatgg ggggccattt gctaatattg
     8461 ctcatggttg taattctgtc cttgctacct caacagcttt gcgattagca gattacactg
     8521 ttaccgaagc aggattcggg gccgacctag gagctgaaaa attcctaaat atcaaagtac
     8581 ctaatcttcc taaagcacca gatgctattg tcattgttgc aaccttgcgc gcccttaaaa
     8641 tgcatggagg tgtcgccaaa ttagatttag ccgctgaaaa ttgtgaggct gttcgcttag
     8701 gatttgccaa cttaaaacgt cacgtggaaa acatgcgcca attcaaagtt ccagttgtgg
     8761 tggctatcaa tgaatttgta gcagataccg aggctgaaat tgctacctta aaagcgcttt
     8821 gtgaagagat taaggttcca gtagaattag ccagtgtttg ggcaaatgga gctgagggag
     8881 gcttagcatt agctaaaaca gtggttcgtg tgattgatca agaagctgct gactacaaac
     8941 gtctctattc agatgaggat actttggaag aaaaagtgat caatatcgtt acccagattt
     9001 atggtggcaa agctgttcaa tttggcccca aggccaaaac acaattgaag cagttcgcag
     9061 aattcggttg ggataaattg cctgtttgta tggctaagac ccaatacagt ttttcagata
     9121 acccatcctt gcttggagcg ccaaccgatt ttgacattac cattcgtgaa tttgttccta
     9181 aaacaggagc aggttttatt gttggtttaa caggtgatgt gatgaccatg cctggtttgc
     9241 ctaaagttcc agctgccatg gcaatggatg tagcagaaaa tggcacagcc ctcggtctct
     9301 tttaggaaaa attgctcgaa taattcgagc aagcgctcct ttcttcccgc tttactgtga
     9361 tataataagg taaatgagat tttggaggga tgattatcaa aaagaaagca aaagtaaaat
     9421 atttgctcca taaagggaaa catggatttt taagaggaat ttttagtaga acaacaatca
     9481 ttgtattatt aattattttg caattagttt tcttatttca atcttatgcc tggatggagc
     9541 agtaccgtgt ttggataacg attcttgaaa gtgtctttgc tattactatt gttttat
//
LOCUS       DAUCWU010000061         9555 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUCWU010000061 DAUCWU010000000
VERSION     DAUCWU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.061"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..99)
                     /locus_tag="VGY24_001309"
     CDS             complement(<1..99)
                     /locus_tag="VGY24_001309"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4284287.1"
                     /translation="MKEMIKAIFYEHKERYGSVRITQELCRRGIHVN"
     gene            complement(234..527)
                     /locus_tag="VGY24_001310"
     CDS             complement(234..527)
                     /locus_tag="VGY24_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4284288.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQSVKMVSSALEIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHAQFEIRKLEKEHKLLQEELALLKKFQVFLKPNRK"
     gene            complement(665..1978)
                     /locus_tag="VGY24_001311"
     CDS             complement(665..1978)
                     /locus_tag="VGY24_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ4284289.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVVVALT
                     SLWSQRCNLSKADQKLLLACASGAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGTKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLIIIPLALASLIFYFGYQFYRFILK"
     gene            complement(1953..2912)
                     /gene="nrdF"
                     /locus_tag="VGY24_001312"
     CDS             complement(1953..2912)
                     /gene="nrdF"
                     /locus_tag="VGY24_001312"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ4284290.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(3116..5275)
                     /gene="nrdE"
                     /locus_tag="VGY24_001313"
     CDS             complement(3116..5275)
                     /gene="nrdE"
                     /locus_tag="VGY24_001313"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ4284291.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEGLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(5295..5513)
                     /locus_tag="VGY24_001314"
     CDS             complement(5295..5513)
                     /locus_tag="VGY24_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ4284292.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5906..6169
                     /locus_tag="VGY24_001315"
     CDS             5906..6169
                     /locus_tag="VGY24_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ4284293.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            6174..7907
                     /gene="ptsP"
                     /locus_tag="VGY24_001316"
     CDS             6174..7907
                     /gene="ptsP"
                     /locus_tag="VGY24_001316"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ4284294.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            8092..9519
                     /locus_tag="VGY24_001317"
     CDS             8092..9519
                     /locus_tag="VGY24_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ4284295.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALIAGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         3025 a         1730 c         1977 g         2823 t
ORIGIN      
        1 attgacatgg atgccacgtc tacaaagttc ttgggtaatt cgtacacttc cgtaacgctc
       61 tttatgttca tagaaaatgg ctttaatcat ttccttcaaa gcttcattct caacatgcct
      121 tgaagaggga cgacgtttta aataagcata gaaacctgaa cgggagactt gaagagtttg
      181 acaggtgtga tgactattta agctaacact attttccttc agaaactgaa attttatttc
      241 cggtttggct tcaaaaagac ctggaacttt tttagaagag ctaattcctc ctgtagcagt
      301 ttgtgctctt tttcaagttt tcttatctca aattgagcat gacgaagtgc gctcccatgt
      361 cctgggaacg cactttctcc atatttttca tattcttgaa tccatcgata aagactattg
      421 ggatgaattt ctaaagctga actaaccatt tttacagact gctcttcctc aaggatgagt
      481 tttacagcag agagtttgaa ggctttatca aaggtttttc ttggcataat gactattcct
      541 cgttttcact taattttgtg tctactttat tataccaagt ccaactttgc ttaaaatata
      601 atgtccaact tttggggtgc agttcattca tccgaagctt tttattaaaa aagtaattaa
      661 ctaatcattt aaggatgaaa cgataaaact gatagccaaa ataaaaaatg aggctagcta
      721 atgctaaagg gataattatt agtaacgatt ggcctgtaat ttcaaccaga aaaactggtg
      781 ccgccaaggg tatcttattg atcacaccta aaaagattgt tgcaccaacc ataatggcta
      841 acgtatttga tagagacaag ggaataaggt actgactaag gatagctagt agtaagccac
      901 tagcacctcc aagcatcata gaaggagcaa ttttaccccc ttttgttcca gaagcaaaaa
      961 ctaaagagat agcgacagct ttggcaacaa gtagccagct aatatagctc agatgtggtt
     1021 cttcatggag aaaaaagagt aatccagctt tgccatttcc taaaatctct gggaagaaaa
     1081 tgctgagtcc tgcgataagg ctaaaagcga tgaggacttt cggaataaac cactgacttt
     1141 tggcatcagc cttaggaagg tgttccaaaa gatgtttgta agcgtgagca aagagggaaa
     1201 ggataagacc agccaggacg gaccaaataa gggttcctag cgtccaatgt tgctgaggca
     1261 ttaggtattg aatctcatgt cgtccttgta ataaagcaac tgtttccaca gccacatagc
     1321 tcgttaggca ggcagcgtat atatttttaa gggaccaacg gttaagaata gcttccaaga
     1381 taaataaaat agtggctaag ggagcattgt agacagcacc aagggcagcg ccagaagcgc
     1441 aagctaataa aagtttttga tcggcttttg acaagttaca tctttggctc caaagagacg
     1501 tcaaggcaac aacgacttcc cgtgaagctc cttctcggcc gacaggagct cccatagata
     1561 cggtcgttaa ttggagccaa ccatgccaaa attgtgtcca gggactaaat gagatgtcat
     1621 cttgaatgat ttgctgaata gattgaatgt cctttccttt tttagccaat aggtgccacc
     1681 caagacctgc aagaagtcca gcaaagagga gtgaaagagc tcgtctttgt ggtggaacac
     1741 tagcaatcat agtgctaaaa gaaccttgac tgaatccaaa actgagggac tgaatcaggt
     1801 gaatggcttt tgttaagata aaagcaacaa ggccagctgt taaacctgtt aaaaagaggt
     1861 aaaaggtcaa gagtaaaaag tgtcgtttca taagactatt ataacaaaag ttaagaaaag
     1921 agactgatag attatcagtc tcgtgagcac tattagagtc cataattata atcgtcatca
     1981 gacatagctt ctacagaacc aagaagatag ccattcccta cctgtgagaa aaagtcatgg
     2041 tttgatgttc ctgttgaaat accattcata acaattgggt taacatcgtt tgctgtatct
     2101 gggaataaag gatcctgtcc taaattcata agagctttgt tagcattgta gcgtaaaaag
     2161 gtcataactt cttcagtcca ccctacgcca tcataaagcg ttttggtata tttttcttca
     2221 ttttcataca gctgataaag gaggtcatac atccaatcac gaaaattctc ttgctcatct
     2281 tctggtagtt cgttaaaacc aagctggaat ttgtaaccga tataagtacc atgtacagat
     2341 tcatcacgaa taattagttt aatgatttca gcaacatttg ctagcttatt atttcccaaa
     2401 tagtaaagag gggtgaaaaa gccagaataa aaaaggaaag tttcgagata tgttgaggca
     2461 actttctttt gtaaggcatc tccattagcg taaatgttat tgataatacg tgctttttct
     2521 tgaaggaact cattattatt agtccactca aagatctctt caatttcttt tttggtgttt
     2581 aaggttgaaa agatagacga ataagattta gcgtgaacag attccatgaa ttgaatattg
     2641 tttaagacag cttcttcgtg aggagtgcga acatcagcac gaatagcttc gacaccagtt
     2701 tctgattgca tggtatcaag tagggttaat cctccaaaaa ccttgccaac aagatctttt
     2761 tcttgaagag aaagtttgcg ccaatcgtct aagtcatttg ataaagggat acgggtatca
     2821 agccaaaatt gttcggtcaa cttttcccaa gttgacttgt caatgacgtc ttcgatttca
     2881 ttccagttaa ttgcttcata atatgtagtc atagaaaaat gatctcctaa agagtagtaa
     2941 agtgcgttaa ggatatcgag ataaaccagt agaaccgacc aaagttctac cggtaataat
     3001 aagtttaaat agattcaaaa gggtaacgaa tgagttagtg agtggtttag gcagatcgcc
     3061 taaagcgact gccgtagaag taatagatgc tttagcatca ttacttctta accaattaaa
     3121 tgacacaaga ttcacattgg tttgcgccca cttcttcccc atcatccgta aaggtacgga
     3181 tatagtagat agatttaatg cctttattga aagcgtagtt tcgtaagatg gataaatcac
     3241 gagtggtttg tttgctttgt gttttccact cataaagctc cataggcaac tcactacgaa
     3301 ggaatagagt taatgacaag ccttggtcca catgttcggt cgcagcggca tagacatcaa
     3361 taactttgcg catgtccata tcgtaagcag atgtatagta aggaatggta tccgtagaca
     3421 aaccatttgc aggatagtag attttaccaa ttttcttttc ttgacgttct tcaattcttt
     3481 gtgtgattgg gtgaatagaa gcagagcagt cattgatata agaaatagag ccatttggag
     3541 caactgctag acggttttga tgataaagac catctttttg aacggcgtcg cgaagagcct
     3601 cccaatctga agcttgcgga ataaagtgat ctttaaagag acttttgacc aaatcagatt
     3661 ttggaacaaa gtgtcctgta acgtatttat caaagtaact accattagcg tacttagagt
     3721 tctcaaagcc aacaaaggta gtttggcgct cacgagcgat gttattggat tcgaccaagg
     3781 tccaataatt caggagcata aagtaaatat cagtaaactc gatggattct ggactgccat
     3841 attcaatatg atgttgagca aggtaagaat gtagtcccat agctccaagg ccaaaagtat
     3901 gtgcttgact gttaccatgt ttaatggttg gaacagcttc aatgcttgat gagtcagtaa
     3961 caaaagttag ggcacgtgtc atggtcttaa tagaacggcc aaagtctggt gaggtcatca
     4021 tgttcaggat attagtggaa cctaagttac atgaaatatc agttcccatt tctacaaact
     4081 cttgcgcatc attgataagg ctaggtgttt gaacttgtaa aatttcagaa cacaagttgc
     4141 tcatgatgat ttttccatcg ataggattag ctttattagc tgtatcaata ttgatgatat
     4201 aagggtaacc agattcttgt tgtaattttg aaatctctgt ttcaagatca cgagctttaa
     4261 ttttagtctt agtaatttta gggttcgcca ctaactcatc gtacatattg gtaatgtcga
     4321 gatagttaaa gggaatgcca tattcttttt caacattgta aggactaaag agatacatgt
     4381 cctcgttttt acgagctaat tcgtagaatt tatcaggaac ggtaatccct agtgacaagg
     4441 ttttaacacg caccttttca tcggcatttt cttttttagt agataagaaa gcaatgatat
     4501 caggatgaaa aacatttagg taaacaacac cagctccttg acgttgccca agttgatttg
     4561 aataagaaaa actatcttca aataatttca taacaggaac aactcctgag gctgcaccag
     4621 catagccttt gattggtgcg ccagcttcac ggaggttaga caaggtaatc ccaactcctc
     4681 caccaatacg ggataattgc aaagcagagt tgatagaacg tccgatagag ttcatgtcat
     4741 cagttacttg aatcaagaaa caagagacca attcaccacg acggcttcga ccagcattta
     4801 aaaaggaagg agtagccggt tggtaacgtt ggttaatcat ttcaacggct aaatcttttg
     4861 ctaagccttc ttgaccatct gcaaaataca aagcattaaa caagacacgg tcttcaaggt
     4921 tttctaaata atgctctcca tcatttgttt ttaaggcgta ttgctggtag aacttgtagg
     4981 ctgccataaa tgatttaaat cgaaaatttt ctgatttgat tatgctatct aattcagtaa
     5041 taaattcagg acggtatttc tgaataaaag ctgattcaat gtaatcattt gagattaaat
     5101 actcaatttt ttccgtaatg gaagtaaaag acatggtgtt tggcagcaca ttttcagcga
     5161 aaaaagcttt taaagcttct ttgtctttat gaagtggaat tttaccatta acaggacggt
     5221 taatttcatt atttaggcga aaatatgaaa tatcgccaag atctttgaga ctcatattat
     5281 ttttccttgt cttattaaat caattctttt aactttgcag gttggaagcc agaaaatacg
     5341 aggttatctg cttcgataac aggagcagat gtgaagccaa gacttttcac ataatccact
     5401 ttttcaggat gttcatcgat attaatttcc tggaagttgg ctccgtgttg ttctaaaaat
     5461 ttcttagtca ttttgcactg catgcaatta tttttggaat atactgtaat catgtcttgt
     5521 ccttctaaaa ttagtaatgg ttccatctta aatggaatta ctataatagc atataaaaaa
     5581 taaagctaaa aagcaagcta taaacttatc tgaaacacaa catattgtgt tgaaataaaa
     5641 aacagaaacc taaatattgt gtcaatctgg attcttatgg ttagagctct agcgttttgg
     5701 tgatagaagc taagcagttt taataaagca ttaaagtctg aaaaataaat tgtgaaaaac
     5761 gtgaaaataa aagaaaataa tttcacattt tttgtcgttt aaatatttaa tcttgaaaat
     5821 aaaaaagcat tgtgctataa tcggttgttg taagggttat catgtgatgg ctcaaaaata
     5881 cacaacttaa aaggagaacc atactatggc ttcaaaagac tttcacattg ttgcagaaac
     5941 aggtattcat gcgcgtccag cgactttgct tgttcaaaca gctagcaaat ttgcttcaga
     6001 catcactctt gactacaaag gtaaagcagt aaatctaaaa tcaatcatgg gtgttatgag
     6061 tcttggtgtt ggtcaaggtg cagatgttac tatctcagct gaaggtgcag atgctgaaga
     6121 tgccattgca gcaattgaag aaacaatgac taaagaggga ttggcataag actatgacag
     6181 aaatgcttaa aggaattgca gcctcagacg gcgttgctgt tgctaaagca tatctactag
     6241 ttcagccgga tttgtcattt gagactgtta cagtcgcaga tacaaatgca gaagaagctc
     6301 gccttgatgt tgcactccaa gctgcacaag acgagctttc tgttatccgt gaaaatgcag
     6361 tagaaagctt aggtgaagaa gcagcagccg tttttgatgc ccatttgatg gttcttgctg
     6421 atccagaaat gatcagccag gttaaagaaa cgattcgcgc aaaacaaacg aatgcagaaa
     6481 caggtcttaa agaagtgact gacatgttca tcaccatctt tgaaggcatg gaagataacc
     6541 catacatgca agaacgtgca gcggacatcc gcgacgttgc aaaacgtgtg ttggctcacc
     6601 ttttaggtgt aaaacttcca aatccagcta caatcaatga agaatcaatc gttatcgcac
     6661 acgatttgac accttcagat actgctcaac ttaacaaaca atttgtaaaa gcatttgtta
     6721 caaatatcgg tggtcgtaca agtcactcag ctatcatggc acgtacactt gagatcgctg
     6781 cggtacttgg aacaaatgat attacaaaac gtgttaaaga tggtgatgtg attgccgtta
     6841 atggtatcac tggtgaagtg attatcgatc caagcgaaga tcaagtactt gcttttaaag
     6901 aagctggtgc ggcttatgcc aaacaaaaag cagagtggtc tctccttaaa gatgcgcata
     6961 ctgaaacagc tgatggcaaa cactttgaat tggctgctaa tatcggtacg cctaaagatg
     7021 tcgaaggtgt taatgacaat ggcgctgaag ctgttggcct ttaccgtact gagttcttgt
     7081 acatggattc tcaagacttc ccaactgaag acgaacaata cgaagcttac aaggcagtgc
     7141 ttgaaggcat gaatggcaaa cctgttgtgg ttcgtacgat ggatattggt ggcgacaagg
     7201 aacttcctta ctttgacctt ccaaaagaaa tgaatccatt ccttggtttc cgtgctcttc
     7261 gtatttccat ctctgaaact ggggatgcca tgttccgcac acaaatacgt gcgcttcttc
     7321 gcgcctctgt tcacggacaa cttcgtatca tgttcccaat ggtagcactt cttaaagaat
     7381 tccgtgctgc aaaagcagtc tttgacgaag aaaaagcaaa cttgcttgca gaaggcgttg
     7441 cggttgctga tgacatccaa gttggtatca tgattgagat tcctgcagct gctatgcttg
     7501 cagaccaatt tgctaaggaa gttgatttct tctcaattgg aacaaacgac cttatccaat
     7561 acactatggc agcagaccgt atgaacgaac aagtatcata cctttaccaa ccatacaacc
     7621 catcaatatt acgtttgatc aacaatgtga tcaaagcagc gcacgctgaa ggtaaatggg
     7681 caggtatgtg tggtgagatg gcaggtgacc aacaagctgt tccacttctt gtcggaatgg
     7741 gcttggatga gttctctatg tcagcaactt cagtacttcg tacgcgtagt ttaatgaaga
     7801 aacttgactc tgctaagatg gaagaatatg caaatcgtgc gcttacagaa tgttcaacag
     7861 cagaagaagt tcttgaactt tctaaagaat acgtttctga agattaaagg gttaaagagt
     7921 tggtttaaac cagctctttt ttgctagcta aaaatacaat tactttttgg tgtgaaatca
     7981 tttgagtcat caaataatta tcagaatttt cagatattta gtgcagccaa atcttgaatt
     8041 atatcggaaa agtgatagaa taagattact atcataaaag gagataaaac gttggcaaaa
     8101 caatataaaa atttagtgaa cggtgaatgg aaactatcag aaaacgagat taccatttac
     8161 gcaccagcaa caggtgaaga gttaggatca gttccagcga tgacgcaggc agaggtagat
     8221 gctgtttacg cttcagctaa aaaggctcta tcagattggc gcgccttgtc ttatgtggaa
     8281 cgtgcagctt accttcataa agcggctgat attttagtac gtgatgctga aaagatcggc
     8341 gcgattcttt caaaagaagt agccaaaggt cacaaggcag ctgtcagtga agttattcgt
     8401 actgctgaaa tcattaatta tgcagcagaa gaagggcttc gtatggaagg tgaagttctt
     8461 gaaggtggta gcttcgaagc cgcaagtaag aagaagattg ctattgttcg tcgtgaacca
     8521 gttggtttag ttcttgccat ctcacctttt aattatcccg ttaacttggc aggttctaaa
     8581 attgctccag cccttattgc aggaaatgtt gttgctctta aaccaccaac acaaggctct
     8641 atttctggtt tgttactagc agaagctttt gcagaagctg gtattccagc aggtgtcttt
     8701 aataccatta cagggcgagg ttctgttatt ggggattaca tcgttgagca tgaagcggtt
     8761 aactttatca actttacagg ttctactcca attggggaag gaatcggtaa attagcgggt
     8821 atgcgaccaa ttatgcttga gcttggcggt aaggattctg ctatcgtttt ggaagatgca
     8881 gatcttgttt tagcagcgaa aaatattgta gccggtgctt ttggttactc aggacaacgt
     8941 tgtacagcgg ttaaacgcgt tcttgtgatg gacaaggtgg cggatcaatt ggcggctgag
     9001 attaaaaccc ttgttgaaaa actaagtgtc ggaatgcctg aagacgatgc tgatattaca
     9061 ccattaattg atacatcagc tgctgatttt gttgaagggt tgattaaaga tgcaactgat
     9121 aagggagcta ctgctttgac agcctttaat cgtgaaggca atcttatttc acccgttctc
     9181 tttgatcatg tgacaactga catgcgtttg gcatgggaag agccgttcgg cccagtatta
     9241 ccaattattc gtgtaaccac tgtagaagaa gccattaaga tttctaatga gtctgaatat
     9301 ggtttgcaag cttctatttt tacaactaat ttcccaaaag cttttggcat tgctgagcaa
     9361 ttagaagttg gaactgttca ccttaacaat aaaacacaac gtggaacaga taatttccca
     9421 ttcttaggcg ctaaaaaatc aggtgcaggg gtacaaggag ttaaatattc tatcgaagct
     9481 atgacaactg ttaaatctgt tgtatttgat atccagtaat aaaaaaaata aacaaaaagt
     9541 taggttaact taact
//
LOCUS       DAUCWU010000062         9116 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUCWU010000062 DAUCWU010000000
VERSION     DAUCWU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.062"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(87..1373)
                     /locus_tag="VGY24_001318"
     CDS             complement(87..1373)
                     /locus_tag="VGY24_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ4284296.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(1475..2401)
                     /locus_tag="VGY24_001319"
     CDS             complement(1475..2401)
                     /locus_tag="VGY24_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ4284297.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDHIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            2600..2884
                     /locus_tag="VGY24_001320"
     CDS             2600..2884
                     /locus_tag="VGY24_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ4284298.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            complement(3045..3473)
                     /locus_tag="VGY24_001321"
     CDS             complement(3045..3473)
                     /locus_tag="VGY24_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ4284299.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     FAEVEAKEKQDDVIIDLNEDDIIIDYTEQDESVSDTLDKH"
     gene            complement(3466..3873)
                     /locus_tag="VGY24_001322"
     CDS             complement(3466..3873)
                     /locus_tag="VGY24_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ4284300.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(3895..4674)
                     /locus_tag="VGY24_001323"
     CDS             complement(3895..4674)
                     /locus_tag="VGY24_001323"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ4284301.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(4671..5603)
                     /locus_tag="VGY24_001324"
     CDS             complement(4671..5603)
                     /locus_tag="VGY24_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ4284302.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(5968..6240)
                     /locus_tag="VGY24_001325"
     CDS             complement(5968..6240)
                     /locus_tag="VGY24_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ4284303.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(6304..6741)
                     /locus_tag="VGY24_001326"
     CDS             complement(6304..6741)
                     /locus_tag="VGY24_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ4284304.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEENGEFIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(6728..8860)
                     /locus_tag="VGY24_001327"
     CDS             complement(6728..8860)
                     /locus_tag="VGY24_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ4284305.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWASKIDLDRHKVNLSLLPPRDTH"
BASE COUNT         2756 a         1986 c         1602 g         2772 t
ORIGIN      
        1 attttcaaaa aaaaaaaaca ggctctcttt tcccaactat ggtcgtatac catttggaaa
       61 aagagggcct ctagtatttc ttgttactat gttctaacag ttttactggt gccatctttt
      121 tgatagaggt tgacaaggcc ttccttgcaa gccctaatca tatcccctgg cttaacttct
      181 ctcggtaaag agatggttaa gagttccatt ggatttgggg cacggtcaat cttttggcca
      241 tccgcatcat gtaagtcctt aacaaccgtt tcaaaatgac ggaaacctgg tccataacat
      301 tcaatccgat cgccttccat gatgacatta cgctgacgaa tggtcgctgt catgctagct
      361 gagtcaaagg caactacttc tccgacaaat ttatattgtg gaattttgcg gcgagcacca
      421 aataattgtt cattttcagt tgggatacca taataaaaac ctgtagccaa ctcgcgctgg
      481 gcaaccttcc ataactcgtc aatcaattcc tctttgatag cataaaaagc ttctgggctt
      541 tccatgtaag catctacagc cgccttgtaa cagttggtta cggttgagac gtagtggata
      601 gatttcattc ggccttcaat ttttaagcta tcaaccccat tttcaatcag gtcaggaata
      661 tggtcaatca tacacatgtc aacagaggac atagaatagt cttctggaat ttcccctttt
      721 aaggagcggc gctctcctcc aaatggcatg tcatacaaat catacttcca gcggcaagac
      781 tgtgagcagc cgcccctgtt ggcatcacgg tgactcatgt ggtttgacaa aacacagcgg
      841 cctgaataag aaatacacat ggctccatgg acaaaggctt caatttccac atctgtccgc
      901 ttgcggattt ctgctaattc agccatattg acctcacgag ctaaaacaac acgtgtcaag
      961 cccatggctt tccaaaattc aaaggtctcg taattggtag atgaagcttg cgttgacaaa
     1021 tgaatttcca aacctggggc ttctgttgaa caaataacaa tcaaggctgg atctgaaaca
     1081 atgaccgcat caagccccat atcacgcagt tgacgaaacc actcgcccgc accaatttcg
     1141 ttcccttcgt gggtaaccat gttggcagca acatagacct tagctccacg cgcatgtgca
     1201 taatcaatgc cttcttgcaa ttcttccata gagaagttac cagcgcggct tcttaggcca
     1261 taggcctgtc ctccaacaaa aacagcatct gcgccatagt caatcgcaac ttttaatttt
     1321 tcaagtgttc cagcaggtga taagacctcg ggacgttttt tcatatgtga catgtgatat
     1381 tctttctatt ttcaagatag gtagattacc tgtgtaatgg tgtcataacc ctgtcttgcc
     1441 ctgaaatagt aaactcactc aagacaagga ttacttattt aacggttttt ggatcgaact
     1501 cataaaagcc agtatctaat ccacggccag ctggatgatg ggcatgcact gcttgatcaa
     1561 gcttttcagc ttcttcttgg gtaaattgcc cagcttctaa taaggtttta gcctgaataa
     1621 acaatttagt aatagcgaca aaatcatcac ctggacagta aatgccatct agcttccaat
     1681 gagttaagcc atgcgcatac agctcaccca atttagacat catgtcaata tcgttattaa
     1741 tgaaaatatg agtgccatga ttgtcttcat aaatggaata atggctgcta gcatctcctg
     1801 gttctgccaa gaaaagtcct cgttcacgac taacctcatc atcaatcttt gtaaaatggt
     1861 aataattttc tagcaaggga cgtttcgagt gatgaatcac cgaggcgccg taaactagca
     1921 cctctgctgg aaattccaaa ttttcagcta aggtgaataa ctctgctgat gggatttccc
     1981 ttgctaaaac actttcaaca gcaccatgtt gcccccagaa atttacctga cggctagaag
     2041 tcacaaaaac agaggtatca taaatcagtt taaagttata gccatccctt ttattgacat
     2101 aaaacacacc tgcatcacca accacaagat aatcaacagc aatctctatc attaagtcta
     2161 aaaagggttt gatgtgatcc atcatatctt gatgcatcaa agcgttgcaa gctactgtca
     2221 actctttgcc tgcatcatgc actagtttgg caatttgtct caattcatca taagaaaaat
     2281 tatggggaag acgtaacccg taattggctt caccaacata aatacgatca acaccagcag
     2341 ctaataaagc tttgacttgc tcaatgcttt cagctgtggc tgtaataatt attttttcca
     2401 tataagcttt attataccaa aaattttaag accttaagca tatttcttgc aaaagcccaa
     2461 acgttaagct agtcccctta ctgttatagc atgataagtc acttgtcatt agtaaatctt
     2521 ttcaaatctt ttaaaaaaag atgacaagaa gagactttta tgatagaatt acttagtatg
     2581 ttaaggaaag gagaggacta tgcctgcacc gttaaagaag cataattcgg ttgaacagga
     2641 tgattcttac caagaatctc aaagtaaatt tcgagatttt caagagttca atggccaaag
     2701 tgcaaagcta aaagaattag tcttctttgc tcgtatcgct agtttcagcc ttattaccgt
     2761 tttatttgca tttgtcctgc tcgtaatgaa tttagcgcca atgttcgctt tcttatttgc
     2821 aagcatcatc agcttaggca ttacttcctt attagccaag gttatcaaat cattactgat
     2881 gtaacttcca gagtccattt tgtatgactc tttttctttt gccaaaaaca aaaaaagatt
     2941 gaagccacaa tcccccccct tcttcagtta gcctaaaact aaccatcggg actagtgatc
     3001 agatccttca atcttttctt cttctatgtt ttaggtcgtt ttagttagtg tttgtctaat
     3061 gtatcactaa cagactcatc ttgttcagta tagtcaatga tgatgtcgtc ttcatttaaa
     3121 tcaatgataa catcatcttg cttttctttc gcttcaactt cggcaaaggt ttcttttgtt
     3181 ttttgcacga aagctgttgt tttatctttg agcaagtcta acatgtcctc tttagttaag
     3241 tcaccagaag ccaacttatc tttcacatca taaaaagtgt cacgtgctaa atggctgtat
     3301 tcactacctt tttctttagc aaattggtgg taatcatctg gactttcttt gtaggcttga
     3361 taagcttttt cagcacgatt tttcaaagcc ttgccttttt ctgttgataa gaagtaagct
     3421 gccgctactc ctgaaacagc tccaatcact aagtttttga aacttttatt catgcttttc
     3481 tccttttttt ccaaatactt ttgaggctac tttaccaact aatgctaatt ttccagcttt
     3541 tgaaacatta cctgtagcat tagttgcttt ttgcccaaaa tgccttgctt gactatttaa
     3601 atctgaaaga ctttctgaca aatcagcaat agcaacaaac agtggatcga tggttgctac
     3661 tttaccatta acatcttcaa caaggatatt agcttttgct aaaatatcat tagtttgatg
     3721 aagtgtcaca ttaacatcac ttgtcaatac agaaatcgtt tttttggctt catcaattgt
     3781 ttctgagact ttctttaata caataatcaa gaaaatcacc aaagcaacaa atgcgagtgc
     3841 aataatcatc agtgagattc caactaagtc cataagtttc tcctttttct atccttattc
     3901 ttgataataa gaaattcctt tttgacggcg tctttttatg acaaagatta agccaataat
     3961 aattaataag gcagacacat attgggagat gcgaatacct aaaaacataa gactatctgt
     4021 tcgcatacct tcaatgacta gtcttccact accataccaa attaagtaaa atgcaaagat
     4081 ttctccatct aatagacttt ttggcttacg acgccacatc ataataatga caaatcccaa
     4141 aagattccaa agtgattcat agagaaaggt cggaatgcgg taacttccct ctataaacat
     4201 ttgcttttga ataaagcttg gtaagtaatt taactggctc acagctttac cataggcttc
     4261 ttggttgata aaatttcccc agcgaccgat agcttgagcg accatgacac ttggtgcggc
     4321 aatatctaaa aaatgaatgg gattgagcac cttgttataa cagtaagcca aaagtacaag
     4381 agctcctgta atgagaccgc cataaatagc aataccgcca ttccaaatgg ctatgatttc
     4441 atctaaatgc ttgacataat aagaccattc aaaaataaca tagtaaattc ttgcaccaac
     4501 aatagcaagt ggaaaagcaa tcagaataaa atcgaaaata gcgtcagatg atattccttt
     4561 tttgggtgct tcttttgatg ctaagtaaac cgccaaaaca agtcctgaca agatacatag
     4621 cgcataccag tggatggcta gtggaccaca ttttaaggca atgggattga tcattgactc
     4681 acctcatttt tggtgattag ttgagttaaa cgatcttcaa aagtttttgt agcatcaaat
     4741 cccatttctt tagcccgatg attcatcgca gcggcttcaa taaccactga aacatttcga
     4801 cctgttttta ccggaatacg aatacgaggg attctcactc ctgagaaagt tatctcctca
     4861 ttaccgtttc cgagacgatc aaaaacttta ccagcctcaa agttttcaag gtaaatggcc
     4921 aattgaactt gtgatgagtc cttcacagca ctagctccat aaagcgacat cacatcaata
     4981 atacctaccc cacgaatttc aagcaagtgg cgcaagatct ctgctggctc tccccaaagg
     5041 gtttcttcat ctttggcata gacatccaca cggtcatctg caaccaaacg atgccctcgc
     5101 ttaactaatt ctaaaccagt ctcactttta ccaattccag aatccccttg gataagaacc
     5161 cccataccat aaatatccat taaaacgccg tgaacactag tacgttcagc taaagaagcg
     5221 tcaagaaaat aagacatttc tccagctaag cggctagttg aaacacggct acttaaaagt
     5281 gaaatacctt cttcttttgc agcttgaacc atttcttttg gaatggctaa atttctagag
     5341 acaaccactg caggagtgtc ctttttgaac atctctttta aaacagaata acggttgtga
     5401 gaagtcatct gtgtaagata agaccactct ttcattccaa atagctgcaa cctttctgga
     5461 gcataataat caaaataacc agtcatttca agacctggac gtgaaatatc tgatgttgtt
     5521 atctcttttg acagcaagtt atctgtagca taaaccacat caagtttaac tttttgcact
     5581 agcatcttta cagtaacggt cataatttct cctttttcaa tattataaca caaatcgacc
     5641 gttagaccct tgcaaatact cattttttca ctactaaatg agaacaacga caccaaaaat
     5701 ggtcttagct ctattaaaaa ctaggtttta tcatttagag atatgagtca aaaataagtc
     5761 aatggcaaac aaatacttta ctagtatcat tgagcaatta gtgcttatta tggtatttaa
     5821 aaaggtatca tcttactaag tcaaaaagca aaaagcctca tcatagctga taagacccct
     5881 cgcttactta catatcaaaa cattttatta tcatcagtta atcacatggt tgctcttacg
     5941 tgatgaatta tcattttaac tgattgttta ccagaaccag ccatcttctt cttcatttaa
     6001 gacatcagcg tctttacgac gacgaggacc tcggccataa cgttcttcaa gcaagtcttc
     6061 cttgtaaggt aagaagatgg ccaacaaaat ataaagcaaa actccaaaac cagttccgta
     6121 aataagaaga gccgctaaga cacgcgctag agcaagatcc caaccatact tatctgctaa
     6181 accagcaata acacctgcaa ccaagcgatt ttttcgctgt ttatagaatt tagtttccac
     6241 gtttctttac ctcctcattc tatgatacta gtttacattg tttttcgcaa aataaaacca
     6301 gtcttacgac tgattttttt ccataagttt cccgtggcaa tttccacaaa catatttagc
     6361 cagattgata cgcctttttc gttgataaac ctgcccacag gtttggcaac tataatgatg
     6421 attagtcttt gatttagagc tagttgggac ataacgaagc ccgtttactt gagctagcag
     6481 atctttgaaa tccctatctt tatgatggta ccctcttcct tcaaaataaa gatggtaatg
     6541 gcagagctca tggcgaacaa ttttccgaaa aataaactca ccgttctcct caagcatcct
     6601 agggttaaaa tccaaatgac catcttttgg aaaaaagcga ccgccagttg tttttaggcg
     6661 cttgttccaa taagccttgt ggtgaaatgg tttgccaaaa tcagccaggg aaacttcttg
     6721 gacataatta gtgagtgtca cgcggtggca ataggctgag attgaccttg tggcggtcca
     6781 agtcaatctt agaggcccaa acagttacca agtcacccac tgaaacaact tgactagggt
     6841 ggttgacaaa ggttttgctc atttcagaaa tgtgaataag cccatcttca tgaacaccga
     6901 tgtctacaaa agcaccaaag tcaacgacgt ttctcacagt tccttcaagc ttctggccaa
     6961 tttccaaatc tttcaaatca aggatatctt gacgtaagat tggtgcttca aagtcatcac
     7021 gaaggtcacg acctggttta aggagatcag caatgatatc tttaagggtt tcctgcccaa
     7081 tagccaatgt ttctgccatt tggggaactt gaactgctgc taaagttgcc ttggcagcgt
     7141 cgtccaagtc ctgaatacca agtaccttaa agagttcttt gactgctggg taggactcgg
     7201 ggtgaactcc tgtgttgtct aaaatatttt tggctcctgg aattctgaga aaacctgctg
     7261 cttgctcaaa cgccttagct cctaaacgcg gcactttttt gatgtcagcg cgtgatgtca
     7321 atgctccatt ttcttcgcgg tatttgacaa tattttctga aatcgttttg ttcaaacctg
     7381 acacatgtgc taataaagat ggactagccg tgttcacatt aacaccgact tggttaacca
     7441 cagtatccac aacaaaacca agattctcac tcaacttttt ctgactcaca tcgtgctggt
     7501 actgaccgac gccaatagat ttaggatcaa ttttaaccaa ttctgctagt gggtcttgca
     7561 agcgacgagc aatagaaata gcagagcgct tctccactgt gaggtctgga aactcatgac
     7621 gggctaactc tgacgctgaa taaaccgaag caccgctttc gttaacgatg acataggacg
     7681 tatttgggaa atctttcaaa acgtctgcta caaaggcttc actttcccga ctggctgttc
     7741 cgtttccaat ggcaataata tcaatctggt aagtctcaat gagctgagtc agtgtttctt
     7801 tagctgcttg aatcttcgtt tggctagctg gtgctacggg ataaataacc tgagtagtca
     7861 acaattttcc agtctgatca acgatagcca attttgcacc tgttcgaaag gcagggtcaa
     7921 acccaagaac cattttccct tttaaaggag acactaacaa aagatgacgg agattttcag
     7981 aaaagaggtg gatagcccca tcttctgcgg catcactgag ttctgaacga acccgtcttt
     8041 ccatagctgg aacaattttc tttttgatgg tctgattgat gacctcttca atataagggt
     8101 tggtttcttt gaaccgcaca ctgaaaaagc gttgcatttt ctccaagtta tgctcaaaag
     8161 acacttttaa gatgcctaac ttttcgccac ggttaagggc taaagtacga tatccttgca
     8221 tgttagacac ttgatcagaa aaatcgtaat agatttggaa aacctttttc tcatccaact
     8281 gctcatcttt aagcgttgat actaagcggc tgtactgcca aatctcattg taggtccaag
     8341 agcgtaattt agcatcttct gacatggcct ccacaaggat gtccacagct cctgctagag
     8401 cttcttgtgg gctggcaaat ccttctgtga caaagggttc tgctgctgtt tcaaggtttt
     8461 gcgcattttg taaaatcaaa cgtgctaatg ggaacaagcc tgcttcacgc gcaatcgtcg
     8521 cttttgtacg gcgtttttcc ttataaggca ggtacaactc ttccaaatca gcgagtttct
     8581 cggttgcttc aatactcgtt cgtaactgat ctgtcagttt tccctgttcc tcaattttag
     8641 cgagaatggt tgctttgcgc tcattcaggg tagtgagaga cttatccata tcaataatgg
     8701 acttaatcac cacttcatct agatttcctg ttacttcttt acggtaacgt gcaataaagg
     8761 gaatggtatt gccttgtgct gttagggcaa gcacctgttc aatttgatgg agactaactg
     8821 acaaagcctc tgcaatgttg tgattattgt tattttccat aatattgcta ttataacaca
     8881 aaccatgtta atgcaaaaaa tgatagcgaa acattgtcct aatataaagt tgcgtaaaga
     8941 atcaaaaaaa gctactttta gtaaataact gacgaaaccc taaataacaa tacctaattt
     9001 taaaaaagaa agaagtaaac aacacttccg ttttggcatt gttctcaaat gatagtataa
     9061 tagacttatc ttgaatgaaa gaaggtcata gatatggcaa ttacccataa aaaaaa
//
LOCUS       DAUCWU010000063         9003 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUCWU010000063 DAUCWU010000000
VERSION     DAUCWU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.063"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            3..929
                     /locus_tag="VGY24_001328"
     CDS             3..929
                     /locus_tag="VGY24_001328"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ4284306.1"
                     /translation="MEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFDKAREVANKE
                     GLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFSKV
                     SSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRKIS
                     ATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLPAD
                     GVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDKIR
                     DMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            972..1376
                     /locus_tag="VGY24_001329"
     CDS             972..1376
                     /locus_tag="VGY24_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ4284307.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            1378..1656
                     /locus_tag="VGY24_001330"
     CDS             1378..1656
                     /locus_tag="VGY24_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ4284308.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            1646..2434
                     /locus_tag="VGY24_001331"
     CDS             1646..2434
                     /locus_tag="VGY24_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ4284309.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAITLEDQ"
     gene            2437..3747
                     /locus_tag="VGY24_001332"
     CDS             2437..3747
                     /locus_tag="VGY24_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ4284310.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            3886..4752
                     /locus_tag="VGY24_001333"
     CDS             3886..4752
                     /locus_tag="VGY24_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ4284311.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            4763..5698
                     /gene="pstC"
                     /locus_tag="VGY24_001334"
     CDS             4763..5698
                     /gene="pstC"
                     /locus_tag="VGY24_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019773503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ4284312.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTVLSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRAIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPESLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            5688..6575
                     /gene="pstA"
                     /locus_tag="VGY24_001335"
     CDS             5688..6575
                     /gene="pstA"
                     /locus_tag="VGY24_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ4284313.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            6591..7394
                     /gene="pstB"
                     /locus_tag="VGY24_001336"
     CDS             6591..7394
                     /gene="pstB"
                     /locus_tag="VGY24_001336"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284314.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            7407..8165
                     /gene="pstB"
                     /locus_tag="VGY24_001337"
     CDS             7407..8165
                     /gene="pstB"
                     /locus_tag="VGY24_001337"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284315.1"
                     /translation="MTEPILQIRDLSVYYNQKKMLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            8233..8886
                     /gene="phoU"
                     /locus_tag="VGY24_001338"
     CDS             8233..8886
                     /gene="phoU"
                     /locus_tag="VGY24_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ4284316.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
BASE COUNT         2800 a         1571 c         1859 g         2773 t
ORIGIN      
        1 aaattgaata tatcaaagat tatcgtgata ttaaccaaga agatgataca gtcttggtat
       61 tgggatattt tgatggatta caccgtggtc ataaagcctt atttgacaaa gcaagagagg
      121 ttgcaaataa agaagggtta aaagtagtcg tttttacgtt tacagagtct ccgaaattgg
      181 ctttttcgcg tttttctcct gagttattgc ttcatattac ttatccaaag aagcgttatg
      241 aaaaatttgc cgattacggt gtaaataaat tatatttggt tgattttact tctaaattct
      301 ctaaggtctc atccgatcat tttataactc attatattaa aaacctgaaa gcaaagcata
      361 ttgttgtcgg ttttgactat aagtttgggc ataatcgtac agatagtgat tatttaacca
      421 gaaattttga aggacaagtt tataccattg aggaaataaa agaagatcat cgaaaaattt
      481 ctgctacatg gataaggaag cttattcaag aaggtaatgt agttaaagca aaccacttgt
      541 taggctatga cttatcgaca aggggaagag tcgttcacgg ggatgcgcgt ggccgtacta
      601 ttggtttccc aacagccaat ctagctccaa ttgacaacac atacttacca gctgatggag
      661 tctatgttac aaatgttatt gttgctaata aaatctatcg ctctatgacg agtttaggta
      721 aaaatgtgac atttgggggg aaagagttgc gtctagaagt taacattttt gactttgacg
      781 aagagatcta tggagaaatt attgagattg tttggttaga taagattcga gacatggaaa
      841 aattcgaggg aatcgaagat ttgactgata gattggaata tgataaacga acagcattaa
      901 attggaaaaa agatagcaaa ttatcttaaa tttttgtata ataaggtaag taaatttgat
      961 agaggaaaaa tatggttacc ttatttttat cacctagctg taccagttgt cgtaaagcaa
     1021 gggcatggtt agtaaaacac gaagttgact ttcaagagca taatattatt acaagtccat
     1081 taagccgtga tgaattaatg tcaatcctct cttttacaga gaatggaacg gaggatatta
     1141 tttcaacacg ttcaaaggtt tttcaaaaac ttgatgttga tgtagaagag ctttcaattt
     1201 cagatttaat tgatttaatt gctaaaaatc caagtcttct tcgtcgccct attatcatgg
     1261 atcaaaaacg catgcaaatt ggttttaatg aagatgaaat tagagcgttt ttatcgagag
     1321 attatcgcaa gcaagaacta cgtcaagcaa cgataaaagc agaaattgag gggtgatatg
     1381 tcaggtaatt attattatcc acttgactta agttggagca ctgaagaaat atcctcagtg
     1441 cttcattttt tgaataaagt ggagttagca tatgaaaaaa aagtagatgc aaaacaactc
     1501 ctagatagtt ataagactta taaaaccatt gtcaaaagta aagctcagga aaagcaaatc
     1561 gaccgtgatt ttcaaaaagt tagtggttat tcgacctatc aagttgttaa aaaagcgaag
     1621 gcaattgaga aaggattctt ttcccttgga aactaaatac gcatttgcaa ggcaaatcat
     1681 taaagaagca gggcttttta tcaagtcaaa aatgtcagag cagctagata ttcaggtgaa
     1741 aacacaattt gatgatctgg ttaccaatgt tgatcaagaa actcaacagt tactgatgga
     1801 tcgtattcat cagacctatc cttgtgatgc cattttagct gaagaaaatg acgttcgtca
     1861 ccccattaat caaggaaatg tttgggtcat cgatcccatt gatggaacgg ttaactttat
     1921 tgtgcaggga tcacagtttg cagtgatgat tgcctattat gaacaaggaa ttggccaatt
     1981 tgggcttatt tatgatgtga tggcagatca attactagct ggtggtggtg attttgaagt
     2041 tactttaaac ggtgataagc ttccagctta ccaagaaaaa cctttagagc gtagcttaat
     2101 tggctgcaat gcaggcatgt ttgccagaaa tgatcgtaac cttgctcatc taattgcaaa
     2161 gactttaggt gtaagggttt atggtggtgc aggtatctgc atggttaagg tcatgaagca
     2221 ggaattactg gcctattttt catttatcca accatgggat tacgcagcag caaaagtact
     2281 aggcgataaa ctgggttatg ttttactaac tattgacgga tatgagcctg attttcaaac
     2341 acgtcaaaaa atcatgtttg ttcctaaatg ccaactcact aggatagcgt cttttctaac
     2401 aaaggactct gccatcacac ttgaagatca atagaaatga tgtcattacc aaaagaattt
     2461 attaacactt atcaagcaat actaggcaaa gaattagaag atttcttggc aagttttaac
     2521 caagagccgg tcaatgcttt tcggattaac cccttaaaga atcaactaaa aacctttgaa
     2581 caccccattc caaatacctt atggggatat tatggtaaac tatctggcaa gtctccagag
     2641 catgtgtcgg ggttggttta ttcccaagaa ccagcggctc agatggtggc acaagtagcg
     2701 gctcctcaaa aggggagtcg tgttttagac ttggcagctg caccaggagg aaaatcaact
     2761 catttattag cttatttaga caatacaggt cttttagtca gcaatgaaat ttctaagaag
     2821 cgcagcaagg ttttagttga aaatatcgaa cgttttggcg ctagaaatgt tgtggtgact
     2881 aatgaatccg cagatcgttt agccaaggta ttttctcatt attttgatac tatcgtcttt
     2941 gatggaccat gttctggtga aggaatgttt cgcaaggacc ctgatgctat tcaatattgg
     3001 catcacgggt atcccgcaga atgcgccaag ttgcaaaaat ctattttaga agacgcctta
     3061 accatgttaa aaccaggagg agaattgata tactccacct gtacttgggc tcctgaagaa
     3121 aatgaagatg ttgtgcagtg gctcttagag acttatacct ttttagaatt ggtcgatgtt
     3181 ccaaaactaa atggaatggt tagtggtatc ggacttccag agactgctag aatgtaccct
     3241 catcgttatc aaggggaagg gcaatttgtg gccaagctaa aagataagcg tcaagaagga
     3301 caaagcacta aactaaaagc acctaaatcc aatttgacca aagaccagtt acgtctttgg
     3361 aaaatgtttg aaaaagacca tctaaacctt accctatcag gaacactaca aacctttggt
     3421 gattacctat acctcttgcc agatggtctt cctgctttag atggccttaa aattgctcgg
     3481 aacggtttag aattaggcgt cttcaagaag aaacgctttg aaccctctta cgccttggga
     3541 ttagctttaa agcctgatga ggtccgatca tctattgagc ttagagagga agactttaga
     3601 ccttatgtgt caggtaacgt cattactttg ccccaatctt atccaacagg ctggtaccaa
     3661 ttactcatta atggcaatgg tctaggattt gccaaggtcg ttgacaagac catcaaaaat
     3721 aatttcccta aagggttaag attttagcgt aaaaaataag attaaaaagt taaatattta
     3781 aagaaaaaat gctttcaaag cataggtaga tatgttacta taaagaatat gtggcactta
     3841 gtttctacat atataccctt tatttttacg aataggaaat aaataatgaa aatgaaaaaa
     3901 aaattctttt tgttaagtct tttggtccta tcaactttct ttttatccgc atgttctagc
     3961 tggattgata aaggtgagtc aataaccgct gtaggatcaa cagcactaca acccttagta
     4021 gaagcagtag ctgatgaatt tggaagcagt aatctaggca agactgtcaa tgttcaaggt
     4081 ggtggttcag gtacagggtt gtctcaagtt caatcaggag ctgtccaaat tggaaatagt
     4141 gatgtctttg cggaagaaaa agatggtatt gatgcttcta aattagttga tcatcaagta
     4201 gctgttgcag gacttgcagt tattgccaat cctaaagtca aggtttccaa tctcagtagt
     4261 cagcagttgc aaaagatttt ttcaggagaa tataccaatt ggaaacaagt tggaggagaa
     4321 gatcttgcga tttcagtgat caaccgagca gcaagttctg gctcacgagc aacctttgac
     4381 agtgttatca tgaaaggggt caacgctaaa caaagtcaag agcaagactc caatgggatg
     4441 gttaaatcga ttgtttcaca aacaccaggt gccatttctt acctttcctt tgcctacgtt
     4501 gattcatctg ttaaatcttt gcaattaaat gggtttaagg caaatgctaa gaacgtggct
     4561 acaaatgatt ggccaatctg gtcctacgaa cacatgtata ccaaagataa accaacaggg
     4621 ttgaccaagg aatttcttga ttatatgttt tcagatgaag tacaacagaa cattgttaca
     4681 catatgggat atatttcgat aaatgatatg gaagtggtca aatctcatga tggaaaagta
     4741 acaaaaaggt aatggaggaa ttatggaaaa tcaggaattg gctaaaaaat tagcctcacc
     4801 atcaaagaat tcaagacttg aaacctttgg ccgcaccatt acgtttttgt gtctggcttt
     4861 gattgttttt atcgttgcga tgattctgat cttcgttgct caaaaaggat tgtcaacctt
     4921 ctttgtggac aaggttaatc tttttgattt cttgtttgga aaggagtggc aaccaagtgt
     4981 gaaaaatgcg gccggcattc cttatcttgg tgctctgcca atgattacag gatccttttt
     5041 ggttaccgtt ttatctgcta ttattgccac cccatttgcc attggcgcag ccgtttttat
     5101 gactgaaatc tcacctaaat atggtgctaa attattacag cctgcggttg agcttttggt
     5161 tggaattcct tcggttgttt atgggtttat cggtttgcaa gtgattgttc cttttatgcg
     5221 cgctatcttt ggtggcacag gttttggaat cctatctggg gtctgtgtct tgtttgttat
     5281 gattttacca acagtgactt ttatgacaac agacagtctg cgggcggtgc ctcgtcatta
     5341 ccgtgaagcg tctatggcta tgggagcaac ccgttggcaa accatttggc gtgttgtcct
     5401 taatgcagct cgtccaggaa tttttaccgc tgttattttt ggaatggcga gagcttttgg
     5461 agaagccttg gctatccaaa tggtagtcgg taactctgca gttatgccag agtctctcac
     5521 aactccagca gctaccctaa cttctgtctt gacaatgggt atcggtaata ccgttatggg
     5581 aacggtccaa aataatgtgc tttggtccct tgccctagtc ttattattaa tgagtctggc
     5641 cttcaattcc cttgtcaaat taatcacgaa agaaagaaag agaaattatg aacgctaaaa
     5701 aagtcgataa agtagcaact ggtactcttt ataccattgc tggaattatt gtagctattt
     5761 tagcttcctt aattctatat atccttgtcc gtggcttgcc acacatcagc tggtcctttt
     5821 taacaggaaa atcgtcttcg tacgaagctg gtgggggaat tggtatccag ttgtataatt
     5881 ccttcttcct attgattgtt actttaatta tttccattcc tttatcaact ggagcgggga
     5941 tttacttggc tgaatatgcc aaaaaagggc ctgttaccaa ctttattaga acctgtattg
     6001 agattctgtc ttccctacca tctgtggttg tggggctctt tggttacttg attttcgttg
     6061 tgcagtttga atatggcttt tctattattt caggggctct tgctttgaca gtctttaatc
     6121 ttcctcaaat gacccgtaat gttgaagata gtttactgca tgttcatcat acccaaagag
     6181 aagcaggatt agctttaggc ctgtcacgct gggaaacggt tttttatgtg gttatcccag
     6241 aagctctccc gggaatggta acaggtattg tcttagcttc aggtcgtatt tttggtgaag
     6301 cggcagcgct tatctatact gctggtcaat cagcaccagc tctggattgg tcaaattgga
     6361 atccacttag tgttactagt ccaatctcta ttttccgtca atctgagacc cttgctgttc
     6421 atatttggaa agtcaatagc gaagggacaa ttccagatgc tactcttgta tccgcaggaa
     6481 gtgcagcagt gctattaatt tttatcctta ttttcaactt ttcagctcgc tttattggga
     6541 agaaacttca ttctaaaatg acagcagcga aataagatat tggagaaatc atgacagaat
     6601 acaattggaa tgaacgtcat atcatcactt ttcctgaaga aacgcttgct ctagctacca
     6661 aggatttaca tgtttactat ggtgcgaaag aagctatcaa agggattgat atgcagtttg
     6721 aaaaacataa aataacagcc ttgattggtc catctggttg tggcaaatca acttatcttc
     6781 gtagtcttaa ccgcatgaac gataccattg atattgctcg ggttacaggt gagattcttt
     6841 atcagggcat tgatgttaat cgaaaagaca tgaatgttta cgagattcgc aagcacttag
     6901 gaatggtttt tcaaagaccc aatcctttcg ctaaatccat ttataaaaac attacctttg
     6961 cccatgaaag agctggtgtg aaagacaaaa aagttttaga tgaaattgtt gagacttcac
     7021 tgaaacaagc cgcgctttgg gatcaagtta aagatgacct tcacaaatca gccttcacct
     7081 tatcaggggg tcagcaacaa cgcttgtgta ttgctagagc catttctgtc aaacctgata
     7141 ttctattaat ggatgaacca gcctctgccc ttgacccgat tgcaacgatg cagctggaag
     7201 aaaccatgtt tgaattgaag aagaattaca cgattattat tgtgacccac aatatgcaac
     7261 aagcagctcg tgccagtgat tacactgcct tcttctattt aggcaacctc attgaatatg
     7321 ataagacacg taatattttc caaaatgcgc aatgtcagtc aacaaacgac tacgtttcag
     7381 ggcactttgg ttagaaaggt aatatgatga cagaacctat tttacagatc agagaccttt
     7441 ctgtttacta caatcaaaag aaaatgttaa aagatgtctc tttggacctt tatccaaatg
     7501 aaataacagc tttaattggt ccttctggat ctgggaaatc aacgctcttg cgttctatca
     7561 accgtatgaa tgatttgaat cctgaagtaa ccattacagg ctctatcgtc tataacggac
     7621 acaatattta tagtccaagg acagatacgg ttgatttacg caaagaaatt ggtatggtat
     7681 tccaacaacc caacccattt ccgatgtcta tttatgagaa cgtggtttac ggccttcgct
     7741 taaaaggtat cagagataaa tcaatcttag accatgctgt tgaaagctct cttaaagggg
     7801 cttccatttg gaatgaagtc aaagatcgtt tacatgattc tgcggttggc ttatcaggcg
     7861 gacagcaaca gagagtttgc atagctagag ttcttgctac cagccccaga ataatccttt
     7921 tagatgaacc tacctcagct cttgatccta tctcagctgg gaaaattgaa gaaacattat
     7981 tgttattaaa aaaagattat acactggcta ttgtgacccg ctcaatgcag caagcctcac
     8041 gtttatcaga tcgaacaggt tttttcttgg agggtgactt acttgagtgt ggtcctacaa
     8101 aagccatgtt tatgaatcct aagcgcaaag aaaccgaaga ctatatttct ggtaaatttg
     8161 gttgagaaag acgtatatgg ttgacccctc tgtgttttta ttataatgac tagattgtag
     8221 gaagaaaaaa tcatgttaag aacgaaattt gaagaagaat tagataaatt gcataaccag
     8281 ttttattcaa tgggaacgga agtattggca caaatcaata aaactgttcg tgcctttgtt
     8341 agtcatgatc gtgaattggc caaagaagtt atcgaagaag atgacactat taataatttt
     8401 gagacaaagc ttgaaaagaa atcgctggaa attattgccc tacaacagcc agtttcgaat
     8461 gatcttagaa tggttattac tgttttaaag gcatcaagtg atattgaacg tatgggtgac
     8521 catgctgctt cgattgccaa ggccactatt cgtatgaaag gtgaggagcg tattccagtg
     8581 gttgaagaac aaatcaacct tatgggaaaa gctgtgaaac agatggtaga agaagccctt
     8641 aatgcatata tcaatgctga tgatacaaaa gcttatgaaa tcgcagcttc tgatgagatt
     8701 atcgatcaat atttccgtaa tatccaaacc ttagctgttg aagaaatccg taagtctcca
     8761 gatgctgttt ttgcaggaaa agaatatttt caagtattaa tgtatttgga acgtattggt
     8821 gactatgctc gcaacatttg tgaatggatt gtttatctta aaactggaaa aattattgaa
     8881 ttataagaaa ttatgctata atgagactgg gaaacagctt gccagtccct ggttttatac
     8941 gaaattgtgc aagttagaaa atgaactaac aagaaaagtt ttctttgctg aatctgattt
     9001 tgt
//
LOCUS       DAUCWU010000064         8668 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUCWU010000064 DAUCWU010000000
VERSION     DAUCWU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.064"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            79..240
                     /gene="sagA"
                     /locus_tag="VGY24_001339"
     CDS             79..240
                     /gene="sagA"
                     /locus_tag="VGY24_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ4284317.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            462..1412
                     /gene="sagB"
                     /locus_tag="VGY24_001340"
     CDS             462..1412
                     /gene="sagB"
                     /locus_tag="VGY24_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ4284318.1"
                     /translation="MSFFTKEKQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSPEEN
                     VRDFAEYGAINAENCNIIIIYVYHYIKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1409..2467
                     /locus_tag="VGY24_001341"
     CDS             1409..2467
                     /locus_tag="VGY24_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ4284319.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2487..3845
                     /locus_tag="VGY24_001342"
     CDS             2487..3845
                     /locus_tag="VGY24_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ4284320.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3820..4491
                     /locus_tag="VGY24_001343"
     CDS             3820..4491
                     /locus_tag="VGY24_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ4284321.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLTELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4488..5171
                     /locus_tag="VGY24_001344"
     CDS             4488..5171
                     /locus_tag="VGY24_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ4284322.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5194..6117
                     /locus_tag="VGY24_001345"
     CDS             5194..6117
                     /locus_tag="VGY24_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284323.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6126..7253
                     /locus_tag="VGY24_001346"
     CDS             6126..7253
                     /locus_tag="VGY24_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284324.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7250..8368
                     /locus_tag="VGY24_001347"
     CDS             7250..8368
                     /locus_tag="VGY24_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284325.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMVSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALIIFSSLLLKPTRL"
BASE COUNT         2716 a         1331 c         1635 g         2986 t
ORIGIN      
        1 actttatttt taaaataagg ttaaaaataa acgactcgtg ttcttatcag ttacttatta
       61 gataaggagg taaaccttat gttaaaattt acttcaaata ttttagctac tagtgtagct
      121 gaaacaactc aagttgctcc tggaggctgc tgttgctgct gtactacttg ttgcttctca
      181 attgctactg gaagtggtaa ttctcaaggt ggtagcggaa gttatacgcc aggtaaataa
      241 tctatttagc atctctatgt gatagtgata ttaaggtaat gagtttgcta gcaactaagt
      301 tatctttggt aacttctgat tatagatgaa tggcatagag gtgttagaaa acatgagaca
      361 aaagtaatct tcttgatgct aacgtctcag gtaattagca ggtactatac agtacctgct
      421 aattactata tgtttagtaa aatgagatag gaaagatagt tatgtcattt tttacaaagg
      481 aaaaacaacc taaagagaat tgtccgccaa taactgttga aaaagcaagg caattgtttg
      541 aatttaatac aaaccacttg tccttatcgg attaccatca tcaaacggtg ctaaaaacgt
      601 caaagcagct agttgctcaa catttaatgc ctaatgcaac cgataatctt agtcaacatt
      661 ttttgatgaa ctataaagct aataataatt atttaggctt ccaagctagt attgtcgact
      721 tttttacaga ttctgccgtt gctaattttt caagtagtta cgtttatgaa agtcaggaaa
      781 agataattcg tttaccaaaa cctaccaaga tatcaactgc tctgtcgaca tgtattataa
      841 aacgaagaag tcatcgtcaa ttttcagata gacaaatgcc tcttcaagat ttatcaaaca
      901 ttctttatta tgcatgcggt gttagttcac aagcatcaat tagagatgga gcatcagata
      961 agataacact cagaaactgt gcttcaggtg gaggtttata ccctattcat ttagtttttt
     1021 atgctagaaa catcagtaaa ttaatagatg gtttctatga atatctaccc tatcagcatg
     1081 cactaaggtg ttatcggcat agccctgagg aaaacgttag agattttgcg gaatacggtg
     1141 ctattaatgc tgaaaattgt aatattatta ttatttatgt ctaccattac ataaaaaata
     1201 cacgtaaata tgggaatcag gcgactgcct atgcttttat tgaatcagga gaaatagccc
     1261 agaatattca attgactgca actgccttaa cttatggaag tattgatatt ggtggttata
     1321 ataaggaata tctccaagaa ttattagatt tagatgggct aggagagcat gtgattcaca
     1381 tgacactcgt aggaactaag gagtctcaat gaaatatcaa cttaatagta atgttcgtgt
     1441 cgtgacattt caagatacat tttgttttag aaaaggaatc tgggacttta atgaagccat
     1501 tctagatttg acacaagaac ctcaaaattt aaagatagtt tatcaagaaa tagtttcaca
     1561 gttggttaag ggagtagcca ttgatactga aacttatgaa aatactttag aacctgagac
     1621 gttcgctaat ttaatggaag tcatttcagg cttgtattac aatgatatgc tgatgcttga
     1681 ggatgactac aatcttgaag aaaatgtcat gaaaatttta atgggtaatt tccgttttat
     1741 ggcgcaagca ggtcatatgg taagcaatga tcctgttttg tttatcagtg attcgactta
     1801 tgttaatgaa tctgctgagt tgctagctga acaactgcat ttacaattgc aagtggctag
     1861 cgatgactta aaaatgttaa ttcagcagac agatgttagt tcgcgtttag atgctttgga
     1921 gcatcgccgt aacatgaatt gtttatcaga agccttgtgc gattaccaaa gtattattat
     1981 ttgtcaagaa cgcttgaata ttatgatgtt gcgacacttg aatgaagtta gcgtagctat
     2041 gaaaaaacaa ttagtgattg gttttgtaga tggaccattc ttgcacactt gtactttgaa
     2101 tcctccccat agtgctgact ttgatagctt agaaagaaga gttttggcac gtttacaaga
     2161 ctcaactctc tatcaacatt ttgctaatca ggttttacca gccacacaag atgtgagcca
     2221 ggcctattta ccattgttaa atgttttaat gaatttagtc gttagcgaag ctttcattat
     2281 tgctcaaaca ggtagttcta aatttgaggg acgtttatta agcatttact taccgacgct
     2341 tgagattcag gtccaagata ttttgaaaat gtctaattca cagacacaag gagctttggc
     2401 aaaattaaag tatgaggatc agcagatatc aacacgtgag attgttaaaa aactccttga
     2461 cgagtaacct ataaggagaa tctattatgt tatactatta tccttctttt aatcatattt
     2521 ttgatgaatt aaaaagttta agtggcaatc gaacaggtat cttaaatcaa tctcaagttc
     2581 cagtttgcaa ccatcctcac gatgtttact taaaaagtat tactggccaa atgccagatt
     2641 atcataaaca atttataggt gagttgagtc aagttagtta tcacattatt ggttacggca
     2701 gttactatga agaagcactt atcaagtatt taggagaaag tattgaacgc tatgctactg
     2761 taattgccgg agacttacta tcagatcgga ttgtctatgc ttcttataat gagttaaaac
     2821 tgttacataa agtgatgcct ttagaatact tgcaagtgtt tacccaagaa cagatcgcat
     2881 tatcttgtga cttgcaaatg atgatgtgcg ataaaatggt aactgagaac gatgtattgg
     2941 gctgggttaa atgtcccatg ttctttgagg acgcagagat gtatgttcca gctcaaatgc
     3001 tttgtgttgg gtataaaaca aatgagactg taggagaaag acggataata ccaggatttt
     3061 ctacgggaac agcttctcac aaaacgttag aagcggctat gtgcaacagt ttgattgaat
     3121 acatccagat cgattcaatg atgcttagct ggtatactaa gaagccttgc cccaagatta
     3181 ttgttgatga tcctgatatt gaagtgattt tagaagaagc tagactggga aaagacagtc
     3241 tatacgacat tattcctatc gatatgactg taggagaaga taatcctctt tatacgtttg
     3301 gaatcattct caaaaataaa tatgatgaag ggccttacct cttgtttgga gtgcaggctg
     3361 ggttagatcc taaacatgct ttgctaagag ggatcatgga agcttcagct atcagctata
     3421 gttactacta taatctttta tatcaaaaag cttcccttgc aaatattgaa tgcgaagaac
     3481 ctttgttttt agatttagat agtaatgttt tctattatgc acatcccaag gaccaagacc
     3541 ataaatggaa ggcctttgaa cctttgattt caggagaagt tctgttaagt gatttggaag
     3601 atcattctgg taaagataaa aaagaagatt tgaagacttt attagcttat gctaagaaag
     3661 tcagtcctaa tgctgtcttt ttagatatta cacctcctga agctctagaa aaaggctggt
     3721 acgtcacgcg agttctgatg ccagaacttc ttgaaatgtg tataccagca tttccttttg
     3781 ctaatcatcc acgaatgaga cagtttggag gtgtaacaaa tgcctttgtc catccaatgc
     3841 cttaatcttt gttttttgtt ggtaactttt tgtccttcaa ttcctatgca agctattttt
     3901 ggaaaggaag atagtggtta tgcatttaat ttaattggtt ttctaagagc aactctgatt
     3961 tatgacattt tggctttggt aagtatttat gttttgtcac ctcaaataac cttgtctctt
     4021 gaaagtattg atagtaagac gttctttatg ggacttgtat tttgcgtctt gatagtattg
     4081 actgaactcg ttttcttaca tggtttacgt tgttggcaaa aaaagcaatg gcttcctgca
     4141 actttctcgt ttgtaggaac gacaaatgat tggtctaaga ttggctatcc tttattacta
     4201 gctttatttg aagagacgat ttatcgtttt ttgtggttta atatattagc ttttcaatgg
     4261 catttaccaa caattattgt tttaattgta actagttttt gctatgcttt aaatcactta
     4321 ttgatgggga aatccatttt ttatgctaag ttggtaacag gcatcattta tggtagtatt
     4381 tacatgctga ctagtcaatt gtggttggtt gtgataatgc atgtaggagg gaacttgcta
     4441 gttgagtgtc ttagccatct tcaaacaaaa aagaagaagg aggtgacatg atgctattgg
     4501 ttttgctgtc gtttttaggt ctaactactc tttggttgac cagttatcga agacgttgtt
     4561 tggctagatg gcatcttcaa tggttagtgg ccttatccta tcaagatttt ttagatgtct
     4621 tactgagtct gtttcaattt gtcattatta tacttgtttt attcttttac tcagctacta
     4681 ttaatctagg agaggttctg acctttttga cacaaactag ctggcattgg cagattcttt
     4741 gttatttagt gttatatctt atggccatca tagagatgac gcttttagtt ttaattctta
     4801 tctttgatgt tttgctccag aaagacagtc gattgctctt taaaaaaata acttggctgc
     4861 cttttagaca agataaacca gtcttaagta tacttttact aggcatagtt attctagtag
     4921 acagtctctt ttatttgggc ttgcttatat tattaggaga acagtcgcta acaagtttag
     4981 caacgcttat tttaggttat gggatagtca aggcttgtcg ctattcaggt tggttaaatg
     5041 tactattagc tttttgtctt ttcactatag ttggactatg ggcagttacg gcaactttat
     5101 tatatggttg gctagtaggt gcgatgatat taacggtgac ttatttgatg attagttgca
     5161 aagagtatta gcaatattaa aaaggagtga tatatgagtt ttgtacaatt aacaaatgtt
     5221 gtcaagtcct acaaaaacgg caagaaagct gtcaatgatg tttccttgtc tattgaagca
     5281 ggtaatattt atggtttgtt aggaccaaat ggtgctggta agtctacctt gattaatctt
     5341 atcttaggct tgatcccttt gagttccggt aaaattactg ttttagggca atcccaaaag
     5401 actattcgaa aaataagttc gcagataggt tatgttcctc aagacattgc tgtttatcca
     5461 gacctaactg cttatgaaaa tgtagaacta tttgggtcac tttatggctt aaagggagct
     5521 cagcttaaaa aacaagttct aaaaagttta gaatttgtgg ggctacactc ccaagctaag
     5581 cagtttccaa gtcaattctc aggaggaatg aagagacggt taaatatcgc ttgtgcgcta
     5641 gttcattcac ctaaattaat catttttgac gaaccgactg tagggattga tcctcaatca
     5701 cgtaatcata ttttagagtc gattcgtttg ctaaacaaag aaggtgccac agttatttat
     5761 acgacccatt atatggaaga agtagaggct ctttgtgatt atatttttat tatggatcat
     5821 ggtcaagtta ttgaagaagg acctaaattt gaactggaaa aacgttacgt tgcaaatcta
     5881 gcaaaccaga ttattgtaac tctaacagat tcacgtcatt tggaactggc agataagcct
     5941 gattggtctt tgatagaaga tggagaaaaa ctcatgttga agattgataa tagtgatatg
     6001 acatcagttg ttcatcagct cacacaggcc aatattactt ttagcgagat tagacataac
     6061 catttgaatt tagaagaaat tttcttacac ttaacaggta agaagttacg agattagagg
     6121 ttactatggt tttatttcat ttaatcaaaa aagaaagttt acagattttt agaaaccgaa
     6181 cagccttatt gatgatggtg atttttccaa ttttgatgat cgttatttta agttttgcct
     6241 ttaaatcaag ttttaatact gcgacaacag tccctaaatt gaccattcgt taccaattag
     6301 agggtgaaaa aacggattac cagaaaaatt ttcttgcttt tttaaaagtt ttaaaccaaa
     6361 aacttcattt agaggctaaa cctagtaatt ctcttgaaaa agatcgacaa agggtcagtg
     6421 aaggagcctt aacggctgtt ttagaagtga agaagaatca gaccattaag gttattacta
     6481 ataatattaa tcagcaaaat gcagatttga tcaatatgct agtaaaaaat tatgtagata
     6541 atgctaaaac ttatgactcg atagcagctc tttatcctca acaattaaat catatcagaa
     6601 agcgaagtgt ggactatgtt aaggtcagtt caatacagac aagtaaagga atgacatcag
     6661 ctgattatta tgctatttcc atgtttacca tgattacttt ttatagtatg atgtctgcga
     6721 tgaaccttgt tttgtcagat cgtcaacaac ggattacaaa tcgtattcac ttaacaggag
     6781 tttctccaag tcttttggtc tttggaaaat taataggtgc tatgttagca acaactgttc
     6841 aattgagtct tttatacatt tttacaaggt ttgttttacg agttaattgg ggcactaatg
     6901 agtggatgct tattggtata acagcctcct tagtctatct ctctgtagct ataggtatcg
     6961 ggctaggcat aagcattaaa aatgaggctt ttttaacggt tgcatccaat actattattc
     7021 ctatattcgc ctttttagga ggcagttacg ttccattgac aacattacac agctctatta
     7081 ttaatcaatt gtcgaatata tctcctatta aatgggttaa tgatagtttg ttctatctta
     7141 tttttggtgg tcaatataat ccgattcctg taactttgat cgttaatatt agtatcggaa
     7201 caatttttat aatattggca ttgataggta tgagaaagca ggtgacgaca tgatttgttt
     7261 tataaaaact ctatttgtta agataaagcg caaaaaaaca agttatgtga cattcttttt
     7321 aatgcctatt ttaacgacac ttttggcttt atctttgagt ttttcaaata ataatcaggc
     7381 taaaattggg atattagata aagataatag tcagatatct aaacaattta ttgctcaact
     7441 aaagcaaaac aaaaagtacg acatttttac caaaatcaaa aaagagcata ttgatcacta
     7501 ccttcaagat aaatctctag aagcggtatt gactattgat aaagggttct cagataaagt
     7561 gttgcaagga aaatctcaaa aactcaatat acgttcgatt gctaatagcg aaattacaga
     7621 gtgggtaaag gcacagacca attatctatt ggaaaattat aatattatag gggatgtggc
     7681 tctaggtaac gaagatactt ttaatagaat tctccaaaaa aaccaacagt taaattatga
     7741 tgttaagcaa gtaactttga cagaccgatc acgtagcaaa gcagtatctt ccacaacgac
     7801 aggatttttg cttattctaa tgttaggtag tacgagcgtg atttacagtg gtattttggc
     7861 agataaatct agtcagttat accatcgatt aatgttatcg aacttatctc gttttcgcta
     7921 tatggtgagt tacgtctgtg taggatttgt tgcttttacg attcaaattg tcattatgct
     7981 gagtttgtta aaagtattta atattagttt ttttgttccg acatcacttc ttttgattat
     8041 ttttttcctt tttagtttat tagcgatagg ctttggcctg ctgattggag ctatcacaca
     8101 aaattcccaa caaagtagcc agttagctaa tcttattgta atgccgactt caatgttggc
     8161 tggctgttta tggccgttgt ctattacgcc ttcttatatg caagcaattg gtaagttatt
     8221 accacaaaat tgggttttat cagccatagc tatttttcaa agtggaggga ccttatctca
     8281 ggcttggcca tacttgttag ctctaatggg aacagcacta gctttgatta ttttttcaag
     8341 tttattatta aaacctacca gactataatt agtcttttgt taaaaaatac tttcctaatt
     8401 agctaactac caggtagccc ttttattggc ttatatgcaa taaaagggtt gctttttgct
     8461 tatattttct ctagtgcact tatcttttgt taaaagcgat tgcgatgtta ctgttgttac
     8521 tgtgtttttt agagagcata ttatcaaaag cgacccctct ataaagttcg tttttgttga
     8581 taaatgggtt gtcaaagtat tttaaaaaac gttttcaata aacataaaaa ctaatgttat
     8641 taaaaaaagt tttgaaaacg tatttatt
//
LOCUS       DAUCWU010000065         8661 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUCWU010000065 DAUCWU010000000
VERSION     DAUCWU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.065"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1470
                     /locus_tag="VGY24_001348"
     CDS             <1..1470
                     /locus_tag="VGY24_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ4284326.1"
                     /translation="YLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFISLLFVVSTL
                     LIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEGDINLTNNNK
                     KLRISKDVDKLIKQSGKTFITSNLNATKHSTREYSYPYIKGTNAYYSFSYIPQALGIL
                     VGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLPMNIYLAASF
                     NQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFTYFLLVCLPL
                     FIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLKEVNVSQQVK
                     NMLESPIVYSSIIIRHMVINLINMNNIFQFGALSYGITNLFPLYVCFFFFVYISNASK
                     ITINIVEKLGIIFVISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFS
                     SQQQKFKQIEDILSDKLAIHVSLLFILAMLMSTIFRYYH"
     gene            1564..2787
                     /gene="pepT"
                     /locus_tag="VGY24_001349"
     CDS             1564..2787
                     /gene="pepT"
                     /locus_tag="VGY24_001349"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ4284327.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQETFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            2828..3316
                     /locus_tag="VGY24_001350"
     CDS             2828..3316
                     /locus_tag="VGY24_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ4284328.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(3303..3500)
                     /locus_tag="VGY24_001351"
     CDS             complement(3303..3500)
                     /locus_tag="VGY24_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ4284329.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            3549..4025
                     /locus_tag="VGY24_001352"
     CDS             3549..4025
                     /locus_tag="VGY24_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ4284330.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            4040..4720
                     /locus_tag="VGY24_001353"
     CDS             4040..4720
                     /locus_tag="VGY24_001353"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ4284331.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    4744..4867
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4882..5412
                     /gene="infC"
                     /locus_tag="VGY24_001354"
     CDS             4882..5412
                     /gene="infC"
                     /locus_tag="VGY24_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ4284332.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            5454..5651
                     /gene="rpmI"
                     /locus_tag="VGY24_001355"
     CDS             5454..5651
                     /gene="rpmI"
                     /locus_tag="VGY24_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ4284333.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            5710..6069
                     /gene="rplT"
                     /locus_tag="VGY24_001356"
     CDS             5710..6069
                     /gene="rplT"
                     /locus_tag="VGY24_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ4284334.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKILADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(6360..8570)
                     /locus_tag="VGY24_001357"
     CDS             complement(6360..8570)
                     /locus_tag="VGY24_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ4284335.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILAQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
BASE COUNT         2826 a         1404 c         1650 g         2781 t
ORIGIN      
        1 tatctttgga aagaaaataa taactatatt tattcgactt ttttgacatt acttctgaca
       61 gttattttat atgccaatag tttaaatcta cctttaaaat ttattagtct tctttttgtg
      121 gtgagcactt tactgatagt gtttttacca aaaaagttag aggttgctac gatcattttt
      181 attttagtat ttggaacgat atctgctatc atatcaccta taaatgatat tcctgatgaa
      241 tatgttcatt attcaaggac agtttatatc tcagaaggtg atattaatct taccaacaat
      301 aacaaaaagt taagaatatc caaagatgtt gataagctta ttaaacagtc aggaaaaaca
      361 tttataacat caaacttgaa tgcaactaaa cacagcacaa gagagtatag ctatccttac
      421 atcaaaggaa ctaatgcata ttatagtttt tcttatatac cacaagcttt gggtatttta
      481 gtaggaaatg cgctggattt acctatactt ttaacatatt atttcggaag attgtgcaac
      541 cttattagct acgctatgct tgcttttata gctatcaagt tgtctggttc ttttaaacaa
      601 gtgatagctg ttgttacttt actacctatg aacatttatt tagcagcatc ttttaatcaa
      661 gacggctttg ctatcggttt agttttagta acaattggat tgtttattaa cttgctctca
      721 tcaaaagata aatctaacta taataccaaa ttcttccttt atttagttct atgtgggtta
      781 ctagttcttt caaaattcac atacttttta ttagtatgcc ttccattatt tattcctaat
      841 gaaaaattcg gaaaaaatac caagttagtc atcttgaaaa aattgggagg actattactc
      901 atttttcttt ttgctgcaat gtggtttaga ctttatggac aggttaaaac cccatatgtc
      961 gcggattttc taaaagaagt aaacgttagc cagcaagtta aaaatatgct agaatcccct
     1021 atagtctata gcagtattat tattagacat atggtaatta atctgattaa tatgaataat
     1081 atttttcagt ttggcgcatt aagctatggt ataacgaacc ttttcccgct ttatgtgtgt
     1141 ttcttctttt ttgtttatat tagtaatgct agtaaaataa ctattaatat tgtagaaaaa
     1201 ctgggtataa tctttgttat ttcagcgatt ataggtgcaa ctgtattagc tatgtacctg
     1261 acttggacac cagtagggtc ctcaactgtc ttaggtgttc aaagtcgtta tctgatagga
     1321 attatcccat tagtgttgct tctttttagt agtcaacagc aaaagttcaa acaaatagag
     1381 gatatccttt ctgacaaact ggctattcat gtttcgcttt tgtttattct cgcaatgtta
     1441 atgagtacta tttttagata ttatcattaa actcttaatt agaggcttta aaaaacttag
     1501 cttgaataag tattttaaag ccttttatgc tataattaaa ttgagaaatt attgaggtgg
     1561 cctatgaagt acgataactt gttagatcga tttataaaat atgtcaaagt gaacacaaga
     1621 agcgatccag acagtgagac tactcctagt acggagagtc aagaaacatt tgccttaacg
     1681 attttaaagc ctgaaatgga ggcaattggt ttgcaagatg ttcattataa tccagttaat
     1741 ggctatctta ttggaactct gcctgccaat aatccaacct tgacgcgtaa aattggtttt
     1801 atagcccata tggatacggc tgatttcaat gctgaaaatg ttaatccgca gattattgac
     1861 aattatcaag gaggggacat tacacttggc agttctaatt ataagttgga tccaaaagca
     1921 tttccaaatc ttaataatta tatcggtcaa acattgatta ccactgatgg gactacactt
     1981 ttaggggcag atgataaatc tggtattgct gaaatcatga cagcaataga attcttaaca
     2041 tcacaaccac aaatagagca ctgtgacatt aaagtggctt ttggccctga tgaagagatt
     2101 ggggtcggag ctgataagtt tgaggtggct gattttgagg tggattttgc ttatactatg
     2161 gatggcggtc ctttaggcga attgcaatat gaaactttca gcgctgcagc tctagaagtg
     2221 acttttttag gcagaaatgt tcatcctggt acggctaagg atcaaatgat taatgccctt
     2281 cagttagcaa tagattttca tgagaagcta cctgctaaag atcggcctga atacacagat
     2341 ggttatcaag gtttctatca cttaacggga ctaactggaa cagttgaaga ggcacgcgca
     2401 tcttacatta ttcgtgattt tgaagaagca tcatttgaag ctagaaaagt gaaagttgaa
     2461 aatatagctc aatcgatgaa tgctcagcta ggaacaaaac gtgtcttggt agagcttaat
     2521 gaccaatact acaatatgaa aaaagttatc gaaaaagata tgaccgctat tgagttagct
     2581 aaagaagtaa tggaagagct tgctatcaag cctgtaatcg aacccatacg tggaggaaca
     2641 gatggctcta aaatttcatt tatgggtatt cccactccta atatttttgc agggggtgag
     2701 aatatgcacg ggcgttttga gtttgttagc ttacaaacga tggagagagc tgtcgatgtc
     2761 attattggac ttgtttgcaa agcctaatta tcatgtaatt gatatttagt ggaaggggct
     2821 tataaaaatg attaaactat ttggtaaaat aaggtatcac tggcagccag agttgtcttg
     2881 gtctattatt tattggtcta ttgcgtttgc acctattttt gtaggtttat ctttgttgta
     2941 tgagcgcact gaaataccta gtcgggtttt tattctattt gctatttttg ctgtcctagt
     3001 tggcattgga ttacatcgtt atttcattat tgaaaataat ggtattttaa gaatcgtatc
     3061 ttttaaattg tttggtccac gtaagctact tatttccact attactaaga ttgaggtgac
     3121 caaatcaaca ctttgtctgc acgttgaaga taaaagttat ctcttttata tgcgcaaatg
     3181 gcctaaaaaa tattttcttg atgccttagc ggttaatcca tacttccaag gagaagttat
     3241 attaagtgat aactttatta aattagacta ttttgaagtt tatcagcatg ataaaaaagc
     3301 ccttactcga gggtaagagc tttagttggg caagatttga cagctaaaat agcgtcctta
     3361 tctgaaggac aaattgactg acttgtttca gatgagcttg aaaatgtgac gataccattg
     3421 tcatgataat caaaaagtga tgaataggtt tggcatagac cacaagcgat gcatttttct
     3481 ggaatgatag atactttcat aactattatt ctaatatgat tttgttaaat taacaagggg
     3541 ggacgatcat ggctaaagaa ccatgggaag aaaaaattgt tgatgatact atagggacac
     3601 gaacacgtaa atcaagaaat gctttcatta gcacgccttg gttgactgct ttattaagtg
     3661 tattctttgt catcattgtt gctatacttt ttattttctt ctatacatca aatagcggta
     3721 gtaatagaca agctgaaaca aatggttttt atggagcatc cactcataaa aaaacaagga
     3781 aagcttctaa cgctaaaaaa acatcaagta gttcaacaac tacagacaca acaccttcta
     3841 gcgaagaaac acttgcttct agtgaaggaa ctggcgaaac ccttactgta ttggcaggtg
     3901 agggggcagc ttctattgca gcccgtgcag gtatttctgt ggagcagtta caagcactta
     3961 atccagagca catgactcaa ggatattggt atgccaatcc aggagatcaa gtcactatta
     4021 aataattgaa gaggaaaaaa tgaaagcaat taaaattgcg attgatgggc cagcttcaag
     4081 tggaaaaagt acagtagcca agattattgc aaaaaatctt ggctatactt atctagatac
     4141 tggtgctatg tatcgctcag caacttatat cgcactgaca catggctata cgggtaaaga
     4201 agtagccctt attttagagg aacttgagaa aaatcctatt tcctttaaaa aagcaaaaga
     4261 tggctcccaa ttagtttttc ttggggatga ggacgtgacc ttagccatta ggcaaaatga
     4321 tgtaactaac aatgtttctt gggtctcagc tttgccagaa attagagaag agctggttca
     4381 tcaacaaaga cgcattgcac aagctggtgg cattatcatg gatggtcgtg acattggaac
     4441 ggttgttctc ccagatgcgg aactaaaaat ctttttagtg gcttctgtcg aagagcgggc
     4501 agagcgccgc tataaagaaa acctagaaaa aggaattgaa tcagattttg agacgctaaa
     4561 agaagagatt gcagctcgtg attacaagga tagtcatcgt aaggtttcac ctctaaaagc
     4621 agctgaagat gcccttattt ttgataccac aggtgtttct attgatggcg tggtgcaatt
     4681 tatccaagaa aaagcagaaa aaatagttga catgtcctag agcctatgtt agaatagtaa
     4741 caatgagaaa agtagaagtg agagcttctc gccttgcgac ttaggttgtc tggccctaac
     4801 atgtcaaatt cctatggaat attatgtgtg cgggcttgat ttatcaagtc cgctttcgtt
     4861 tttctctaaa aaaataaaga ggtgaagatc atagctaaaa aggatctatt cattaatgat
     4921 gaaattcgcg ttcgcgaagt tcgtctcgtt ggtcttgaag gtgaacaatt aggtattaaa
     4981 ccattgtctg aagcgcaatc attggctgat gcttcaaatg ttgatttggt tttaatccag
     5041 ccacaagctg ttcctcctgt tgccaaactt atggactatg gaaagttcaa atttgagtat
     5101 caaaagaaac agaaagaaca acgtaaaaaa caaagcgttg tgactgtgaa agaagttcgt
     5161 ctcagtccag ttatcgataa aggtgacttt gaaacaaaac ttcgtaacgg tcgtaaattc
     5221 cttgaaaaag gaaataaagt taaggtttct attcgtttca aaggacgtat gattactcat
     5281 aaagagattg gtgccaaggt actagctgac tttgctgaag cgactcaaga tattgctatc
     5341 attgagcaac gagcaaaaat ggatggacgc caaatgttta tgcaacttgc accaatttca
     5401 gacaagaagt aattgtcaca gtttcattat atctttaaga ggagaatact aaaatgccaa
     5461 aacaaaaaac acaccgcgca tcagctaaac gttttaaacg tacaggttca ggcggtttga
     5521 aacgcttccg cgcctttaca tcacaccgtt tccacggaaa aactaaaaaa caacgtcgtc
     5581 atcttcgtaa agctggtttg gttagctcag gagatttcaa acgtatcaaa gcaatggtta
     5641 ctggtcttta attttaaagg cagtagacat ttacgagtat tataagaatt atttgaagga
     5701 gaactattaa tggcacgtgt taaaggtggc gttgtttcac gcaaacgtcg taaacgtatt
     5761 ttaaaattag ctaaaggtta ctatggagca aaacacatct tgttccgtac tgcaaaagag
     5821 caagtgatga actcttacta ctatgcatac cgtgatcgcc gtcagaaaaa acgtgatttc
     5881 cgtaaactat ggatcactcg tatcaatgcg gctgctcgta tgaacggttt gtcatactca
     5941 caattgatgc acggtttgaa acttgctgaa atcgaagtaa accgtaaaat acttgctgat
     6001 ttagcagtag ctgatgcagc agctttcaca gctcttgctg atgcagccaa agcaaaactt
     6061 ggcaaataag aaacgctata cctaaaaaat aactttctat tacctataga aagttataaa
     6121 gaaacaaaaa tgaaggagac gatggacgtc ttctttttat tatactcaat ataataaaaa
     6181 gcagtttccc atgttttatt accagtaatg tgggatattt agatggtagc aaggagtttt
     6241 atagctgatt tattttcttt aggtctaatt agcatatttt gattactgat aaacttgaat
     6301 atcgccaata aaaaagaagc aaaattatta ttttgcttct agtcagatca ttaaattgat
     6361 tattctttat ttttggggtt atcctctttt agttcagaag ttgccgcgtc agcttctacg
     6421 ggatttagtt ctaagtaaga tggtgcttta aaaagtttct gagttgattt atgaccattt
     6481 ttactgtata aacttgttga ctctgatccg agttttgctg aaatatcttt cagttgtttt
     6541 aactgcttag tgtagataaa ctggttagga tcaatagctt ttagaccatt ttgagtatca
     6601 aagcgcagga gatcacccgt ttgaatagcg tcgctagctg ctagttgttg agcaacagca
     6661 gagcgaattt ccttagtttt ggctatagtc atttcatcgg gattagtgat ttctaaacct
     6721 gtttgcgtgt tgtacagacg tccactatag ttagtgtatt caggagtcat ataagtccca
     6781 gatgttcgtt gtgccacaat ctggctattt tgaggtgata ataaatcttg tcctaattga
     6841 acaaactgac tagtgtcaat accaagtatg tggagtaaag taggaagagc atcgatttca
     6901 ccgccaaagg tttctttgat actaccattc gtataacctg gaatatggat catatacggg
     6961 actcgttgta gcatagcatt atcatattca gaccaagttt cagaatcttt accaagaagt
     7021 ggagcaagac tagaattacg agaatttgaa ataccataat ggtcaccata taaaacaaaa
     7081 atagaattgt cgtacaaacc agtggctttc aagtaatcaa aaaaagattt aagggcagcg
     7141 tctaaataat tggcagtagc aaagtaacca ttgatcgttt catcgtctgt tttggctaaa
     7201 ggaaaacctt cttcactact ttcgcctttt agactagtat aaggatagtg attactcaca
     7261 gtgataaact tggtataaaa aggttgttgc atctgttcaa gatattttat ggaatcctta
     7321 aacatgtact tatcatttaa cccatattga aaagagttct tactattttg tttagagaag
     7381 tagctagaat caaaaaaata attatagccc cattgtttat aagcattatt gcgattccag
     7441 aaagttccaa cgttaccatg aaagacagcg ctggtatagc cacctttttg ggctaaaata
     7501 cttggagtag caaattgtgt attttcacca ccgtagttca ccataaaaga accactattc
     7561 aaaccaaata aggaattctc catcatggtt tcagcatcag aagttttacc tgctttaacc
     7621 tgatgaaaaa agttagggaa tgctaaggta gcatttgagt gataaagcga gttgataaaa
     7681 ggcgttacct catattcttt atctccttct tttagtttat aatctattaa gaattgttga
     7741 aagctttcta aatgaagaac aataatattt ttgccttttc caattccaaa atactgagga
     7801 tcaggtgctg cataatgtcc tttgacataa gttttaacat caattaattc ttctgcagta
     7861 gctccatttc gttctttttg agcttgatag gtttgattgc cgctatagag ggtaaacgct
     7921 ggtagtccta aagctctcac aatataagta tttgaaaagc cacgtgttaa tagttccgga
     7981 cgatcaattt ctgctaaaaa aagattaata gaaagcaata gactagataa agcggtgatg
     8041 gcaaatgctg cgcgcttgtt aaaaggacgt tgatcatttt tgattttttt agtgatagat
     8101 aaactaatta gaataataaa atcaagaata tagataatat cccagatgcg taaaaggttt
     8161 aaagcagagt cccctaatcc agcggagact ttactactag ctagcatggc actcactgta
     8221 ataaagtctg aaaattctcg gtaatatata gaatttgata tcaagagaat gttaaggata
     8281 atataaacca cccaagaaca gatataaaag gctcgggtat tcttaacgta aagagcaacc
     8341 cccaaaagta aaaaagctag tgggattgga ttgataatag ttagaaagac ttggtatata
     8401 tttcccaaat ctagtgaaaa gtctgtatga tatgcccaca gggtctttat ccagtaacaa
     8461 aagagtagag taattataaa acccaatctt gtgttgataa aacctgtgat aagtgtttta
     8521 aatttcttca cagtagcaac ttccttatcg ttttttccta aagatgtcat ctccaaataa
     8581 aatctcagat taaattatac caaaaatgtg aagctaatgc ttgttgtaag ttcaaattta
     8641 gtaggatttt ttatcagatt t
//
LOCUS       DAUCWU010000066         8616 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUCWU010000066 DAUCWU010000000
VERSION     DAUCWU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.066"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            610..999
                     /locus_tag="VGY24_001358"
     CDS             610..999
                     /locus_tag="VGY24_001358"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ4284336.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFDPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1093..2145
                     /locus_tag="VGY24_001359"
     CDS             1093..2145
                     /locus_tag="VGY24_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ4284337.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2284..3816
                     /locus_tag="VGY24_001360"
     CDS             2284..3816
                     /locus_tag="VGY24_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284338.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGVLAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVSPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3809..4873
                     /locus_tag="VGY24_001361"
     CDS             3809..4873
                     /locus_tag="VGY24_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284339.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4875..5831
                     /locus_tag="VGY24_001362"
     CDS             4875..5831
                     /locus_tag="VGY24_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284340.1"
                     /translation="MSLVTIFALLMSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(6045..7763)
                     /locus_tag="VGY24_001363"
     CDS             complement(6045..7763)
                     /locus_tag="VGY24_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ4284341.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVVGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(7938..8507)
                     /locus_tag="VGY24_001364"
     CDS             complement(7938..8507)
                     /locus_tag="VGY24_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4284342.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCCKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
BASE COUNT         2664 a         1409 c         1740 g         2803 t
ORIGIN      
        1 cctaatttaa ataatcaata atttctcatt taaattaggt ttgcttaaat agtaaagagt
       61 tatttttaaa ttatagataa ctaatattat actagggtag cccctctact cgagtaacct
      121 tttttaatgt attttaactg aaaaaggctt tcataatttt aaaaaaatgt taaaataggt
      181 gtataacaac tgattaccaa gagattatta tcattatctt ctaagttgtc attgttatgg
      241 ttgatcaagt gattgaaaag tcaatattta taattttaca aatatctgta tcaatccatg
      301 ttgaatagtc tatgcttcta aggtgtcgac gttaatgtac tgacgggacc gttatagtct
      361 ttctttttgg agaaacgtcg gatgccagtg aactagttga agttaaagat gttaattcaa
      421 acagtgctga agagattgat atcgttttga acggtcgttt acttcaaagc aaagaaatta
      481 ccagtctatc ggagatgata taagtgctaa agttagatca cgttaattat ctatcaagtc
      541 gggtgcatat ttcgttaaag tatctacagg ggtttcaata gatgtagcta ttatgaaagg
      601 tgatgttaaa tggggactac tgatttagtt agttgtgctg tacaagctag tgagtatgct
      661 tatgtccctt actctcattt tccagtagga gcagctttaa aaacaaaaga tggaacgatc
      721 tatactggct gtaatattga aaatgttagt tttggtctga caaattgtgg agagcgaaca
      781 gctatattta aagccatatc tgatggtcat aaagaattgg ttgaaattgc tatttatgga
      841 gaaactatgc aacctgtatc tccttgtggg gcttgtcgtc aggtaatggc agaatttttt
      901 gatccttctt ctttagtcac tttaattgct aaaaatggtc aaactgttga aatgactgtt
      961 ggagatttac tcccctattc ttttacagat ttaatataat ctgctttaat ttctgttatc
     1021 tagccgtctt ggctttatat ttactttcag caacttattt gctagaaatc ttttttagga
     1081 gggttcatat tcatgaacaa gaaatttatt ggtcttggtt tagcgtcagt ggctgtgctg
     1141 agtttagctg cttgtggtaa tcgtggtgct tctaaaggtg gggcatcagg aaaaactgat
     1201 ttaaaagttg caatggttac cgatactggt ggtgtagatg acaaatcatt caaccaatca
     1261 gcatgggaag gcctgcaatc ttggggtaaa gaaatgggcc ttcaaaaagg aacaggtttc
     1321 gattattttc aatctacaag tgaatctgag tatgcaacta atctcgatac agcagtttca
     1381 ggagggtatc aactgattta tggtatcggc tttgcattga aagatgctat tgctaaagca
     1441 gctggagata atgaaggagt taagtttgtt attatcgatg atattatcga aggaaaagat
     1501 aatgtagcca gtgttacctt tgccgaccat gaagctgctt atcttgcagg aattgcagct
     1561 gcaaaaacaa caaaaacaaa aacagttggt ttcgtgggcg gtatggaagg aaccgtcata
     1621 actcgatttg aaaaaggttt tgaagcagga gttaagtctg ttgacgatac aatccaagtt
     1681 aaagttgatt atgctggatc atttggtgac gctgcaaaag gaaaaacaat cgcagcagct
     1741 cagtatgcag caggtgctga tgttatttac caggcagcag gaggcactgg agcaggtgta
     1801 tttaatgaag caaaagctat taatgaaaaa cgtagtgaag ctgataaagt ttgggttatt
     1861 ggtgttgacc gtgatcaaaa agacgaagga aaatacactt ctaaagatgg caaagaagca
     1921 aactttgtac ttgcatcatc aatcaaagaa gttggtaaag ctgttcagtt aatcaacaaa
     1981 caagtagcag ataaaaaatt ccctggagga aaaacaactg tctatggtct aaaagatggc
     2041 ggtgttgaaa tcgcaactac aaatgtttca aaagaagctg ttaaagctat taaagaagcg
     2101 aaagcaaaaa ttaaatctgg tgacattaaa gttcctgaaa aatagtgttt aagaacaaga
     2161 agcgcgacct ttgtcggtcg ctctttttaa actgagataa tttttatctc agtaaagttt
     2221 gtgataggtg atagatcata ggctttactg aaataaaaga ttgcattgaa aggaaatgat
     2281 cctatgacac agcatgtcat tgaaatgaga gaaatcacga aaaaatttgg tgattttgtt
     2341 gcaaatgacc acattaactt aaatgtcaga aaaggtgaga ttcatgcctt attgggtgaa
     2401 aatggggcag gtaaatcaac tttgatgaat atgctagctg gtctcttaga gcctactagt
     2461 ggagaaattg tcattaatga caagccagtt caaattgatt cgccatctaa atcagctaag
     2521 cttggtatcg gaatggtaca tcaacatttc atgttagtag aagccttcac ggttgctgag
     2581 aatattattt taggaaatga agtagtaaaa aatgggtgct tagatcttaa ccaagcatcg
     2641 aaagatatta aggtactatc cgaaaaatat ggccttgcaa tcaatccatc agctaaagtt
     2701 tctgatattt cagtaggagc acaacaacgt gttgagattt tgaaaacctt gtatcgtggt
     2761 gcagatattc ttatctttga tgagccaact gccgttttaa cacctgcaga aattaaagaa
     2821 ttgatgacta tcatgaagaa tctcgtgaaa gaaggaaaat caattatttt aattacccat
     2881 aaactggacg agattcgtgc tgtcgccgac cgtgtcacag ttattcgtcg tggtaagagt
     2941 atcgaaacag ttgatgtggc aggagcaact tcacaagatt tagctgagat gatggtggga
     3001 cgctcggttt cctttacaac tagcaaaaaa gctgctgaac caaaagatgt ggtcttatcc
     3061 atcaaaaatc ttgaagtaga tgaaaataga ggggttctag cagttaaggg gctatctcta
     3121 gatgtccgag ctggtgaaat tgtagggatt gctggaattg acggaaatgg tcaatctgag
     3181 ttaattcaag ctattacagg tttacgtaaa gtcaaatctg gaagtattat gattaaaaac
     3241 aatgaagtca ctcatttgtc ttcacgcaaa attactgagt tatcagtggg tcacgtgcca
     3301 gaagatcgtc atcgagatgg tttgatacta gatctttctt tagctgaaaa tacagcctta
     3361 caaacctatt acaaacaacc tctaagtcaa aatggaatct taaattatac taaaattaat
     3421 gactatgctc gtcaattaat gaaagaattt gatgttcgcg gagccaatga gctagtacca
     3481 gctagaggat tttctggtgg taaccaacaa aaggccatta ttgctcgtga agtggaccgt
     3541 gatccagatt tacttattgt tagccaacca acacgtggtc ttgatgtagg agctattgaa
     3601 tatatccata agcgtttgat taaagaacgt gataaaggta aagctgtcct cgttgttagt
     3661 tttgaacttg atgaaatatt gaatttatct gatcgaattg ctgttattca tgatggtaaa
     3721 attcaaggta tcgtatcgcc agaaaatacc aataaacaag aattgggtat tttaatggca
     3781 ggtggttcaa ttcataagga ggaaggtcat gtctaaaaat gcgcaaaaaa ttgctgtacc
     3841 attgatttct gtcttgctag gatttttact tggcgctatt attatggtta tctttggtta
     3901 tgatcctatc tgggggtatg aaggtttatt ccaaattgcc tttggtagtg ttaaaaatat
     3961 tggagaaata ttccgttcaa tggggccatt gattttaata gcgcttggct ttactgttgc
     4021 tagccgtgca ggcttcttta acgttggttt gtctggacag gctttagctg gatggatttc
     4081 agcaggttgg tttgcactct taaatccaga tatgcctcgt ccattattga ttcttatgac
     4141 agctcttatc ggaatgattg ctggtggtat tgctggagct attccaggga tacttcgtgc
     4201 ttatcttggt acaagtgaag ttatcgttac gattatgatg aactacatca ttttatatgt
     4261 cggaaatgct attgttcaac gtggttatcc tgagagtgtc aaacaaagta ttgattcaac
     4321 tattcaagtt agtgataatg ctagttatca aacgcattgg ttatcagccc taactaataa
     4381 ctctcgtatt aatattggta ttttctttgc cattattgcc attgccctaa tctggttctt
     4441 gcttaacaaa actactttag gttttgagat tcgttcagtt ggtctcaacc cacatgcttc
     4501 agaatacgca gggatgtcat caaaacgtac tatcatttta tcaatgatta tctctggagc
     4561 tcttgcaggt cttggaggag tagttgaagg tctaggaacc tttgaaaatg tttttgtaca
     4621 aggatcttct ttagcagttg gttttgacgg tatggcagtt agcttattag cagcaaattc
     4681 accgcttgga atatttttct catcattcct atttggtgtc ttaaatattg gtgctccagg
     4741 aatgaatatt gcaggcattc caccagaact tgttaaagta gtaactgcat caatcatttt
     4801 ctttgttggt tcacattacc tcattgaacg ttacattatt cgccctaaaa aattagtgaa
     4861 aggtggtaaa taagatgagt ttagtaacaa tttttgcctt attgatgtca tctatgctta
     4921 tttatgcaac accactcatt ttcacaagta ttggaggaac tttctcagag cgctcaggcg
     4981 ttgttaatgt tggtttagaa ggtatcatgg tcatgggagc gttctctggg attgttttca
     5041 acttagagtt tgctgaaaca tttggaaaag caacgccttg gatagccgtt ttagtcggtg
     5101 gtattgttgg tttgattttt tctttgattc acgctgtagc aaccatcaac tttcgagctg
     5161 accacattgt cagtggtaca gtgttgaact tgttagcacc ttcttttgct gtctttttgg
     5221 ttaaagctat gtacggtaag ggacaaacag acaacattca acaatctttt gggaagtttg
     5281 atttcccagg tttatcacaa attcctgtga ttggtgatat tttctttaaa aatactagcc
     5341 ttattggtta cttcgccatt gctttttcgt tctttgcttg gtttttgctt tataaaacca
     5401 ggtttggtct gcgtttacga tcagttgggg aacaccctca ggcagctgac acacttggta
     5461 ttaacgtcta tttgatgaaa tactatggtg tgatgatctc aggttttctt ggtggaatag
     5521 gtggagctgt ttatgctcag tcaatctccg ttaactttgc tgtaacaact atcttaggtc
     5581 ctggatttat tgctttggca gctatgatct ttggtaaatg gaatccagtt ggcgccatgc
     5641 tatctagtct tttctttggc ttatcgcaaa gtttagccgt tattggtgct caattaccat
     5701 tactagaaaa gattccaacg gtttacttac agattgcacc ttatatggta acgattatta
     5761 ttttagctgc tttctttggt caagcagttg caccaaaagc tgatggaatc aactatatta
     5821 aatctaaata acgttatatt agtaagcctc ttagtgaggt ttaacagcac tcctaaagca
     5881 agatagttag gtctagcttt aagggtgctt ttttggggac ttaccgcaag tttggattgc
     5941 cagtcaccat cacaatagaa gcaagaatag actaaaaaga actaagtcag cggcttagtt
     6001 ctttttgctt gtcttttgtt tttactgata gctcttaagt tgttttagcc aacaaaggta
     6061 ttgatttctg tttcaatgtt agcaattttt tcttgagcga tgtcaagagt atcacctatt
     6121 gtggcaatat agaacttgat ttttggttca gttcctgaag ggcgaactgc aatccatgaa
     6181 tcatcagcta acgtgtactt caaaacatta cttggaggag ttgttaagtt agatattgtt
     6241 ccatcatttt tagtagcggt ttgtttttgg aaatcttcta ataatacaat atcagtattg
     6301 ttaaattgtt ttggaccatt ttcacggaat ttgtccataa tcttcttaat ttcagcagct
     6361 ccatccacac ctgaaagtgt tactgaaatc gttttttcgg caaaatagcc atattctttg
     6421 taaatttcat caataccatc tgcaagtgtt aaaccacgcg aacggtaata agctgcgatc
     6481 tcagcaacca aaagaactgc ttgaatagcg tctttatcac ggacaaatgg tttaataaga
     6541 tatccgaagc tttcttcaaa accaaacata taggtgtggt tatgtttttc ttcaaactct
     6601 tgaattttct cagcgatgaa tttgaatcca gttaaaacgt taaacattgt agcgccataa
     6661 ctttcggcaa tttttgttac taattctgtt gatacgattg atttagcgag agcagcattt
     6721 tctggaagtg ttcctgctgt tttatgagct tccaggatgt atttcgcaat aatcgctcca
     6781 atttgatttc cagaaagatt tttataagaa ccatctggtt ggcgaatttc aacgcctagg
     6841 cggtctgcat caggatcggt agctactaag acatctgcat caacttctcg tccgagttct
     6901 tcagcaagag caaaggcagc ttggctttca gggttaggcg atttaacagt agaaaagtct
     6961 ggatctgcct tagcttgtga ttctactaca acaacagatt caaaaccggc ttgagcaagg
     7021 gcacggcgtg tcaacatctc accagttccg tggagaggag tatagacaat tttcatgtct
     7081 tttccaaagt tattgattaa atcttgattg atattaacat ctttaacctc tctaagatat
     7141 tccatatcaa catcttcacc gacaacttga ataagacctg atgatttgct ttcatctaaa
     7201 tcagcaagtt caactgcaaa cggattttca atagcacgga taaagtctgt tagtgcatca
     7261 gcatcagctg gtggtagttg acctccgtct tgtccataga ctttataacc attgaaaggt
     7321 gctggattgt ggcttgcagt aaccataata ccagcatagg catttaaatg gcgaacagca
     7381 aaagagagtt ctggtgttgg acgaagagct tcaaatacgt aagatttaat tccatgctgt
     7441 gccaagacct gtgcagactc aaaagcaaat tctggagaga agtggcgcga atcataagcg
     7501 atggctacac cacgtttttt agcttcctct cccttagttt cgattaattt tgctaatcct
     7561 tcggttgctt gacggacaac ataaatatta atacgatttg taccagcacc aatataacca
     7621 cgcataccag cagtaccaaa ttctaagttt gtgtaaaaag catcttcttt tgttttttca
     7681 tccattgata gtaattcttg acgaagatag tcaggaagct gctcaaaatc taaccatttt
     7741 tgaaaatttt ctgtgtaagt cattgaaata ctccttcaat atttataaac gctttcatta
     7801 tatcattttg aagggaaaaa aacactaatt atttgcagct gtttacaaaa ctttttccat
     7861 attttcaagt agctaatgta agaaagttaa aataaccaag tttttaaaga gaaaaactcg
     7921 gttattgtga aagctaatta cctttttagt cttgatagtg ttggaataag tacaaaggta
     7981 ataatagctg agatgatcat ctcaacaata gcgtttgatg agatgatggc tgttagtagt
     8041 gctttaatat ttccatcaaa aactgtcgcg aaaaaaataa atatcccaga taatacaaat
     8101 atagtatttg ttagagaacc aatgataccg gatactatta aaccaaactg attaggaatc
     8161 aatttacagc aataataagg ggtaatacca atcaaaatac gtgggataat agcaattatt
     8221 aaagaagcga aagttccatg atcgacaaaa ggtgaaaaga gataatttgt aggcagtaga
     8281 atgatggtat ttgtaatgac acttataatc cccattaatc ctcctaaaat agcacctatc
     8341 cttgggccat ataagacaga agcaataata actgggatat ggacaagcgt tggtttgatt
     8401 ggaataggcc aaatattaaa gacaagactg ctcacaaaat gaatgactaa catgatggca
     8461 aaaaagatag caatacgaga aatatctgct gatttacggt gattcatttt actctccaaa
     8521 aaatataata cttaattcta aaaaggtgat cagaaaaacg actgttaaag tttccaaatc
     8581 gtatcaatag tagctaagat aacatcaata tcagcc
//
LOCUS       DAUCWU010000067         8425 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUCWU010000067 DAUCWU010000000
VERSION     DAUCWU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.067"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            72..974
                     /locus_tag="VGY24_001365"
     CDS             72..974
                     /locus_tag="VGY24_001365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ4284343.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNSANALAQELNLPIAKTVFEASNNSQDIPMIGSDEVGNGSYFGGIAV
                     VASFVDPKDHSFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            985..1578
                     /gene="lepB"
                     /locus_tag="VGY24_001366"
     CDS             985..1578
                     /gene="lepB"
                     /locus_tag="VGY24_001366"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4284344.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTIFN"
     gene            1636..4089
                     /locus_tag="VGY24_001367"
     CDS             1636..4089
                     /locus_tag="VGY24_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ4284345.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKTADALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNLIYTAITRSKSKLIMLGEFAAFDYAIKNEGDKRQTYLVERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTAALEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDVALFFQKKS"
     gene            4180..4662
                     /locus_tag="VGY24_001368"
     CDS             4180..4662
                     /locus_tag="VGY24_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284346.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIIFCTQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILAQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(4755..5849)
                     /gene="dinB"
                     /locus_tag="VGY24_001369"
     CDS             complement(4755..5849)
                     /gene="dinB"
                     /locus_tag="VGY24_001369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ4284347.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            6059..8386
                     /gene="pflB"
                     /locus_tag="VGY24_001370"
     CDS             6059..8386
                     /gene="pflB"
                     /locus_tag="VGY24_001370"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ4284348.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLVYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDKNGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
BASE COUNT         2614 a         1778 c         1564 g         2469 t
ORIGIN      
        1 gtctctgttt tctttgttaa ataaacttat gaattaacca tagtcaacta ttttatgtta
       61 aaataattat tatgaatacc ttagttttga aaatagatgc tatactgagt aaacatttaa
      121 aaaaacagct agccccctac accatcagta gccaaaatac ctatgtagcc tttgctgcta
      181 aaaaaaatgg ggttactgtt ttgctgtaca aatccggaaa attagttctc cagggaaaca
      241 gtgcaaatgc tttagctcaa gagcttaatc ttccaatagc taagacagtt tttgaagcaa
      301 gcaacaatag ccaagatata cctatgatcg gcagtgatga agtgggtaat ggctcttatt
      361 ttggtgggat tgctgttgtg gcaagttttg tcgatcctaa agatcattcc ttcctgaaaa
      421 agcttggggt tgatgattct aaaaaattat cggataaaac cattcaacag attgcgcctc
      481 tgttagagaa acaaatacct catcaatcac ttctcctatc acctaaaaaa tataacgaat
      541 tagttggtaa aagtaaacca tataatgcca tttctatcaa agttgctctt cataaccaag
      601 ctattttttt gttgttacaa aaaggcattc aacctaaaca aattgttatt gatgctttca
      661 ctagccaatc aaattatgaa aagcatctaa aaaaggaaaa aaaccatttc ccaaatcctc
      721 taacctttca agaaaaggca gaaagtcact atttggctgt ggctgttagc tctatcattg
      781 ctagaaatct atttttagac aatctcgatc aattaggtca agatttaggt taccaactcc
      841 caagcggtgc tggttcagct tcagataaag ttgctagcca actcttagcc gcttatggta
      901 tgtctagctt agaatatagt gctaaacttc acttcgccaa cactcacaaa gcgcaagcgc
      961 ttctcactaa ataaaaggaa tcatatgaaa cagtttatta aagaatgggg cccattcact
     1021 ctctttttaa ttctctttgg tctatctcgt ctttttttgt ggcaggctgt taaagtagac
     1081 ggccattcta tggacccaac tctagctcat ggcgaacgcc ttatcgtttt taatcaagct
     1141 aggattgatc gctttgatat tgtagttgct caggaagaag aaaacggaca aaagaaagaa
     1201 atcgtaaaaa gagttattgg attgccaggc gataccattt cttataatga tgacacactt
     1261 tatattaatg gtaaaaaaac agttgagccg tatttggctg agtatctaaa acaatttaaa
     1321 aacgataaac tccaaaaaac ttacgcctat aataccctat tccaacagtt agcagaaaca
     1381 tctgatgctt ttacaactaa ttctgaggga caaacacgct ttgagatgag tgttccaaaa
     1441 ggagaatacc ttcttcttgg tgatgatcgt attgtttcca gggatagtcg cgaagttggt
     1501 agtttcaaaa aagaaaacct tatcggtgaa gtgaaagctc gtttttggcc actcaataaa
     1561 atgaccatct ttaattagaa aaaacctgcc tttgggcagg ttttcagtta tcttgtataa
     1621 atagaaagag ttactatgga atacgttttt acaggtactg ttgatcgtat catttttgaa
     1681 aaccaggcta atttctttaa aattctcctc cttgccattg aggatacaga cagtgacatt
     1741 gacgactttg aaatcattat cacaggaacg atggctgaca ttattgaagg agatgactac
     1801 accttttggg gagaattgac ccagcaccct aaatatggac agcaactcaa actaagccgt
     1861 taccaaaaaa tcaaacctag ttcatctggt ttggttaatt atttctctag cgaccatttt
     1921 aaggggattg gtaaaaaaac agcggagaaa atcattgcac tatatggtca taataccatt
     1981 gaccatattt tagaagaccc aagcaaatta gaaactatct ctggcctatc taaggctaat
     2041 cgtcaagcct ttgtcgctaa actaaaattg aattacggca cagagcaact gattgctggt
     2101 ctcgtcgaac ttggccttag caaccgtttt gcctttcaag cgtttgaaaa gtataaagaa
     2161 gaggctcttg accttgttaa agaaaatccc tatcagttag tcgaagatct acaaggtttt
     2221 gggtttaaaa cggcagatgc tctcgctgaa aacttaggga ttgaaagcga ctctccgaaa
     2281 cgttttcgtg cagccctcct tcactgtctc ttggaagaat ccatcaatcg aggagatact
     2341 tatgtccaag cacgacaatt attagacttt gccatcacac tccttgaaga tgcacgtcaa
     2401 gtagaatgtg atcctgctgc tgtggctgaa caactaagcg agttaatcat agagggcaaa
     2461 atcaaaaaca gtgacaccaa actgtttgat gctagccttt attttgctga agaagggatt
     2521 gctaacaaca tctctcgtct cttagatact cctttaagtc agtcatttag tcatgatacc
     2581 attcaaacaa ccatccaagc cgttcaaaaa gactttgcca tcacctatga ccaagtgcag
     2641 caagaagcca ttactaaggc cttaaccagc aaggtctttc tcctaacagg tggtcccgga
     2701 acaggaaaaa caactgttat tcgaggcatt ttgcaggctt acgcgaacct gcatcagatt
     2761 gatttggata aaaaagacct tcctatcttg ttagcagctc caacaggtcg ggctgcacgt
     2821 cgcatgaatg agttgactgg acttcctagc gcaaccatcc acaggcactt aggcctcaat
     2881 ggcgacaacg attaccaagc catggaagat tatctagact gcgacctgct gattgtcgat
     2941 gaattttcaa tggtggatac ctggcttgcc aaccagttgt taggagcgat taactcaaca
     3001 actcaagtga ttattgtcgg agatagtgac cagcttcctt cggttggacc cggtcaagtc
     3061 ctgtcagatc ttttaaaagt caatagcctg ccccaaatcg ccttgcaaaa gatatttcgt
     3121 caatcccaag aatctactat tgtcaatttg gcagaccaga tgcgtcgagg aatcttagct
     3181 gctgactttc gtgataaaaa agctgaccgt tcttattttg aagcccaagc agcctttatc
     3241 cctgacatga ttcaaaaaat tgtcctatct gccattaaaa gtggcatccc tgctgaggaa
     3301 attcaaattt tagcgcctat gtacaaagga caagctggca tcaatcatct taatcaactc
     3361 atgcaagaac ttctcaatcc cttgcaagga caaacagaat ttctgttcaa tgatacgcat
     3421 tttcgtaaag gtgataaagt cctgcacttg gtcaacgatg ctcagttgaa tgtctttaac
     3481 ggggatattg gttatattac agatttgatt cctgctaaat ataccgaatc taagcaagac
     3541 gaattaatct tagattttga cggtagcgaa gtaacgtatc ctagaaatga atggctaaaa
     3601 ttaaccttag cctatgccat gagcattcat aagtcacaag ggagcgagtt tcaagtggtg
     3661 attttaccca tcacacgcca aagcggccga cttttgcaac gaaatttaat ttatactgct
     3721 atcacccgtt ctaaaagtaa gttgattatg ctaggtgaat ttgcagcctt tgactatgct
     3781 attaaaaatg aaggggataa acggcaaact tatttggtgg aacgcttcca agaacaatcc
     3841 gacttagcct cctctcaacc taaccaagag ctaaaatcaa aagagcagac ctcccttttt
     3901 tctaatacgg cagcccttga ggacgactct caaaaatctt cctctcaatc aacaaactct
     3961 aaccccactg agaactctca gtcagataat gatgatttta ggttaacacc tgagaattat
     4021 tcgactatcg attcgatgat agggcttaca gaatcagatg ttgccctctt tttccagaaa
     4081 aaatcctaga aaaacggaca tttttgatta atctatggta taatattttt gtattaaaca
     4141 cattgtacaa tttcggatta aggactctag gaggattata tgatttctta tgaaaaagtg
     4201 cgtcaggcac tcaaaacgtc tactattgcc attattattc taaacgggct tggagttgta
     4261 ctgtcattaa tgggatttgc agggatcttc tacttacaaa gccaactcaa gaatgaagca
     4321 tttcgggccc aattaacaac tgagcaattg gcacagctac aaagcagcat gacaccattt
     4381 atgattttct tatctgtctt aaatgtcctt gctattattg ccattatcat cttctgtact
     4441 cagaacttat ccaaactaaa acaaggtcta acggttagtt atattcctta tatcttaggc
     4501 cttatccttt ctgttatcgg cttggttaac cagttcacaa caaccatgtc aatggttgga
     4561 actatcctta tccttgcaca agctgctctt tatggctttg ctttttacaa agccaaaacc
     4621 cttaacgaaa aaggtgatga tacagaccaa gctatgctgt aataaaataa tggaccaaag
     4681 aagctgatta gggaaatccc taatcagctt cttttttctc caaaatgagg cggacggata
     4741 gagcactctt tctcctatag gtccaaggaa atatcagcta ccttatcctc caaattggtc
     4801 atggttaccc ctaacaggcg gataccagca ggattttcgc ttaagctgtc aaaaatatca
     4861 ccagctactt gctcaatttg tgcggcattt ctggttaatt ctggcaaggt gacacgtttt
     4921 gtcaaggtgg taaaatcagc ataacgcact ttgagcacaa tggtcttgcc taacttttta
     4981 tggtcttgta agagagcggc cacgcgctta gcatttttac tgatctctgc cttgatgtct
     5041 gtttcttgat aaagcagttt agcgtaggtt ctctcactgc caattgactt gcgtatccga
     5101 tcagacttga caggagaatt gctgatgcct ctcgctttac ggtaaaggtc aaaaccaaac
     5161 cgaccaaaat gatcaatcaa ggtcatttca ggaactgcca acaaatcctg tcctgtataa
     5221 atccccatgt catgcagttt ttcaactgat tttttaccaa caccatgaaa cttttcaatg
     5281 gggagtttgg ctaaaaagca cagggcatct tctttcaaga ctagggtgag gccatgaggt
     5341 ttttcaaaat cactagccaa tttagccaaa aatttgttat aagacacacc tgctgaacag
     5401 gtcaatccta cttctttcca gatatcatgc tgaatcagct tggctatttt gacggctgat
     5461 tttataccca acttattgtt ggtgacatct agataggcct catcaatgga catgggctct
     5521 accacatcag tataacgctt aaaaatacga cggatctggt ccccaactgt tcgatacttt
     5581 tcataatttc ctgaaataaa aatggctttg ggacaacgct cataagcttc cttagagctc
     5641 atggccgaat gaatgccata ttttctcgct tcgtaattac aagtggaaac aactccgcga
     5701 ccacctgttt ctcttggatc tttcccaatc acaacaggct ttccttttaa agcagggtta
     5761 tccctttcct caactgcagc aaaaaaggca tccatgtcaa tatggatgat ttttcgtgac
     5821 gtgtcattaa tcagtggaaa aataagcata agtccctcct ttctctatta taccttttag
     5881 gctatctaaa gccaaaaaaa atacattcta atacagtttg tgactgtttc aacaatctgt
     5941 attataaaag aatcttaatt ttgtcaagaa ttttgcttgg gaaaacgttt cctatgtgtt
     6001 aaacttagaa ttgtaaatgt aacagatcgc tttgttacta gaatttaagg agaaatttat
     6061 ggcaactgtt aaaaccaata cagatgtttt cgaaaaagct tgggaaggct ttaaaggaac
     6121 tgactggaaa gaaaaagcaa gtgtctcacg ttttgtacaa gctaactaca ccccatacga
     6181 tggcgatgaa tctttcttgg ctggtgctac tgaacgttca cttcacatca aaaaagttat
     6241 cgaagaaaca aaagcacact atgaagctac acgtttccca tacgatactc gtccaacttc
     6301 aattgctgac attcctgctg gtttcatcga taaagaaaat gaattaattt atggtattca
     6361 aaacgatgaa ttgttcaaat tgaacttcat gccaaaaggt ggtatccgta tggcggaaac
     6421 tacccttaaa gaaaacggct atgaaccaga cccagcagta catgaaatct ttactaagta
     6481 tgtgacaact gttaacgacg gtatcttccg cgcttatact tcaaatattc gtcgcgctcg
     6541 tcatgcccac actgtaactg gtcttccaga tgcttactca cgtggacgta tcatcggtgt
     6601 ttacgctcgt cttgctcttt acggtgctga ctacttgatg caagaaaaag tcaatgactg
     6661 gaatgctatc actgaaatcg atgaagaatc cattcgtctt cgcgaagaag ttaaccttca
     6721 ataccaagct cttggtgaag tggttaaact tggtgacctt tacggagttg atgttcgtcg
     6781 cccagcacaa aacgttaaag aagctatcca atgggtaaac atcgctttca tggctgtatg
     6841 tcgtgtgatc aacggtgctg ctacttctct tggacgtgtg ccaatcgttc ttgatatctt
     6901 tgcagaacgt gaccttgctc gcggtacctt tacagaatca gaaatccaag aatttgttga
     6961 tgattttgtc cttaaactcc gtaccgtaaa atttggtcgt actaaagctt acgatgccct
     7021 ttactcaggt gacccaacat tcatcacaac ttctatggct ggtatgggta acgacggtcg
     7081 tcaccgtgtg actaagatgg actaccgttt cttaaacact cttgataata tcggtaactc
     7141 tccagaacca aacttaacag ttctttggac tgaccaactg ccagaaacat tccgtcgcta
     7201 ctgtatgaaa atgagccaca aacactcttc tatccaatat gaaggtgtga caacaatggc
     7261 taaagaaggt tatggtgaaa tgtcatgtat ttcatgttgt gtatcaccac ttgacccaga
     7321 aaatgaagaa caacgtcata acatccaata cttcggtgct cgtgtaaacg ttctcaaagc
     7381 ccttcttact ggtcttaacg gtggttacga tgatgttcac agagactaca aagtgtttaa
     7441 cgttgttgaa ccaatcacat cagaagtcct tgtgtacgac gaagttatgg ctaactttga
     7501 gaaatctctt gactggttga cagatactta tgtagatgcg cttaacatca tccactacat
     7561 gactgataaa tacaactacg aagcagttca aatggccttc ttgccaacac accaacgtgc
     7621 taacatggga ttcggtatct gtggtttcgc taacacagtt gataccttgt cagctattaa
     7681 atacgcaact gttaaaacta tccgcgataa aaatggctac atttatgact acgaagtaac
     7741 aggtgacttc cctcgttacg gtgaagatga cgaccgtgtt gatgacatcg cgaaatggtt
     7801 gatggaagct taccacacac gtcttgcaag ccacaaactt tacaagaatg ctgaggcttc
     7861 agtatcactt cttaccatca cttcaaacgt tgcttactct aaacaaactg gtaactctcc
     7921 agttcaccgc ggcgtcttct taaacgaaga tggtacagtt aacactagcc aagtggaatt
     7981 cttctcacca ggtgctaacc catctaacaa agcaaaaggt ggctggttac aaaaccttaa
     8041 ctcacttgct aaactggaat tctcacatgc taacgatggt atctcattga ctactcaagt
     8101 atcacctcgt gctcttggta aaacatttga cgaacaagtt gacaacttgg taacagtgct
     8161 tgacggttac tttgaaaatg gtggtcagca cgttaacttg aacgttatgg accttaacga
     8221 tgtttatgac aaaatcatga acggcgaaga cgttatcgtg cgtatctctg gttactgtgt
     8281 caacaccaaa taccttacac cagaacaaaa aactgaattg actcaacgtg tcttccacga
     8341 agtgctttca atggatgatg ccgctgaagc tatttcaagt aaatagtagt atctaaatga
     8401 tcaataactt ataaaaaaga acagc
//
LOCUS       DAUCWU010000068         8243 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUCWU010000068 DAUCWU010000000
VERSION     DAUCWU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.068"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..556
                     /locus_tag="VGY24_001371"
     CDS             <1..556
                     /locus_tag="VGY24_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284349.1"
                     /translation="ALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNH
                     QIDKKSKDLIDKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFG
                     DSMEPTYLNGEVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSD
                     KFAPYSEEPRIIGKIIANFRPLEI"
     gene            complement(691..2253)
                     /gene="ffh"
                     /locus_tag="VGY24_001372"
     CDS             complement(691..2253)
                     /gene="ffh"
                     /locus_tag="VGY24_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ4284350.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2268..2609)
                     /locus_tag="VGY24_001373"
     CDS             complement(2268..2609)
                     /locus_tag="VGY24_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ4284351.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(2698..3396)
                     /locus_tag="VGY24_001374"
     CDS             complement(2698..3396)
                     /locus_tag="VGY24_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4284352.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3479..4912)
                     /locus_tag="VGY24_001375"
     CDS             complement(3479..4912)
                     /locus_tag="VGY24_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284353.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKKPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDYFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLISWFMEPIHFPPNSPVIVAGNIIN"
     gene            5079..6641
                     /gene="guaA"
                     /locus_tag="VGY24_001376"
     CDS             5079..6641
                     /gene="guaA"
                     /locus_tag="VGY24_001376"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ4284354.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSDTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(6683..7906)
                     /locus_tag="VGY24_001377"
     CDS             complement(6683..7906)
                     /locus_tag="VGY24_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ4284355.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYTIRKQLKNKH"
BASE COUNT         2455 a         1579 c         1456 g         2753 t
ORIGIN      
        1 tgccttattg catctattca atgtgacatc tgattatttc gacccaaact atagattgct
       61 aacgccttat aaccagctga caatatctaa taaagaaaaa gtaattggct attcagagcg
      121 attgttaaat catcaaatag acaaaaaatc taaagatctc atagataaac catcacaatt
      181 atatgcttat cgggtctatg aaagtttatc tgctggtact ggttactcct attttggtga
      241 tggtaacttt gatgttgtct tttacgatga acaattagaa tacgattttg cgtcttgggt
      301 ttttggagat tctatggagc caacttattt aaatggcgaa gttgttctta taaaacaaaa
      361 tagttttgat tacgatggag caatttatgc agtcgaatgg gatgggcaaa catatatcaa
      421 aaaggtattt cgtgaagatg agggattacg tctagtgtcc ttaaataaaa aatattctga
      481 taagtttgct ccctatagcg aagaacctcg cattattggc aaaattatcg ctaattttag
      541 gcccttagaa atttaagtta gattgattag gttatttcaa attttaaatg aactcatcat
      601 gcatgtaacg cggtcttcca taattttaat aatcttgtat acaagataaa tgaatttcac
      661 atcaaaaaga gacgaaaatt tccgtctctt ttattttcgt tttttctttg ctttttttag
      721 tttgttagct tgacgtttca tggcctgttt catggcaaat tgtccaattt tccccttgaa
      781 ccctttacca aatagttgac tcatatccat atcaccacca aggccagata aatcaggcat
      841 accaccttgt cccatcatcc cttctaagga agacatatct ggcataccag caggcatgtt
      901 tttaggaaga ttattgggat taatgcccat atctttcatc atcttagaca tatcgcccga
      961 cataacccct tgcatcatgc ttttggcttg gttaaagtct ttgataaatt tattaacttc
     1021 cacaaagctg ttcccggaac cagctgcaat acgacggcga cgactcgggt ttagtaagtc
     1081 agggttttca cgttcagcgg gggtcatcga agagacaata gcacgtttgc gagcaatttg
     1141 gttttcgtca actttgatat tggccaaagc tgggttacca gccattcctg gaatcatttt
     1201 caagaggtct tccataggcc ccatattttg cacttgatct aactgttcaa tgaaatcatt
     1261 gaaatcaaag gtgttctcac gcattttttc agctaactct aaggattttt tctcatcata
     1321 ttcctggcta gctttttcaa tgagggtaag caaatccccc attcccaaaa tccgactgga
     1381 catgcggtct gggtgaaatg tttcaatatc tgtgattttt tcaccaatac ctgtaaattt
     1441 aattggtttc ccagtgattt cacggacaga tagcgctgca cctccacggg tatcaccatc
     1501 aatctttgtt aacacaaccc ctgtgatgct caactgatga ttaaactcgt aggctacatt
     1561 ggcagcttct tgaccaatca tgctatctac aaccaaaaga atttcatttg gttgtgctaa
     1621 agccttgaca tcgcgcagct cttccatcag tttttcatca atttgcaagc gacccgcagt
     1681 atcaatcaag acatagtcat tgcggttttc acgagcttgt tccaaacctt ttctgacaat
     1741 atcgactgct gagtggtctg tccccatatc aaaaacaggt acattgattt gttgccccag
     1801 tgtttttaac tgatcaatgg ccgctggacg gtaaatatcg gcagcaatca tcagtggtcg
     1861 cgcgttttct tctttaatta atttattagc gagttttcct gcaaaagttg ttttaccagc
     1921 cccttgcaaa ccgaccatca tgataatagt tggaattttt ggagatttat caatctctgc
     1981 ggtttctgag cctaaaatac tcgttaattc ttcattaaca atcttaagaa tttgttgtgt
     2041 tggatcaaga gtatcaataa tctcatggcc aatagcgcgc tcacgtacgc gcttaataaa
     2101 ggttttaaca actggtaaag cgacatcggc ttctaaaaga gctagacgaa tctcttttgt
     2161 cacttcttgg acatctgatt ctgataattt ctttttaccg cgaatgtgtt taaacacatc
     2221 ttgcaaacgt tgggttaagc tttcaaaagc catagtcaaa actcctctta ctctctatta
     2281 tcaatactgg ttagaatgga aattttttct tgcaaatact catcatgagg gtagtgagca
     2341 atcatgtcgt caaagatttc acttcgaaca acataatccg aatacatatg aagtttcatc
     2401 tcataagtct ctaaaatttt ttccgtacgc ttaatattat catagacagc ctgacgactg
     2461 acaccaaatt catcagcaat ttcagctaag ctataatcat cggcataata cagttcaata
     2521 taattcattt gtttatctgt tagcaaagct gcataaaatt caaaaagggc attcatcctg
     2581 tttgtttttt caatttccat aatgttcatt ttatcatact atgattgttt ttaacagtag
     2641 caaattaatt gccttgtgct gttcgtgtaa aaaaagaagt cataaatgac ttctttacta
     2701 attattttct aagtaaaatt caaaacggtc accgatatac tgactgcgga catactcaaa
     2761 aggtttgccg tctgtaaagt aagacacctg agttagagcc aaaatggcat gtcctttagc
     2821 aacttccaag taggaggcta cacgctcact tgctagctta gcgtagattg tctgtttact
     2881 tttaccaatc tcgtaaccat ttgctatcaa tgagtgaaaa aagtgttctg taatatcagc
     2941 tcgtttaacc gtttttataa atttttctgg gattgaagca atttcgtaca ccaaagggac
     3001 actgtctgca tagcggatac gttccattcg aatgaccaag tctgttttgt ctaagttcaa
     3061 ttctttaact tcggtatcac tagctaattg cttttgatag gataataatt tagaggaggg
     3121 ttgtcgtcct tgggagcgta taatttctgt gaaacttgtt gttcctcgca ttttttcttg
     3181 aacacgatga ctggcaacat aagtaccact tcctatccgc ctttcaagaa tcccttcttc
     3241 tactagtaat gtaatagcct gccgtagagt cattcgacta acggtgaaat gttccgctaa
     3301 atgtcgttca cttggtaagc gactaccaat tggccagatg cccagatcaa tgtctttttt
     3361 aattgcatca tgaattttta tataagctgg taacatgcca cactcctcaa gtcggttatt
     3421 catctatttt atcatatttt tgatgaaaat tgaattgtaa atattgaggt atttggcatc
     3481 aattaatgat attacctgcg acaataaccg gtgaatttgg tggaaaatga ataggttcca
     3541 taaaccaact aatgagcttc gctttagtaa caatggatga ggtaaaaaca ttatttaaaa
     3601 ccctaataac tcctactttt gaggaatgct gaccaataga accactatag gctctcaggc
     3661 aaccagtcgg atctatataa ggtaaaaatt gattttggct tacagtaata ttgtgacata
     3721 gatgtcgagt ttggttaaat gcttcatagt ttggagcaat attactaagt agacctccat
     3781 cccatgcaaa atgatgaaca gcagcatcaa actgaatgac ttctgaaaaa taatcatgcc
     3841 cttgagcctt tgatagcagc tgttggtctt gtactaattc tggcgcatag ccaataaatt
     3901 ggtttttctc aaacaatgaa ttttgtagag atcctaaatc aaaaatatga ctattacgct
     3961 tatgaaccat agtaaatttg ttatcgtaaa tatgccaatc agtaccatga tcagccataa
     4021 tcataaagtg gtctcctttt ttcaaatcat ttgctttgaa atgcactcca gtcaacacga
     4081 gattacgaac accttgttca gcctttggtc ctgtagggaa tccaaaccac aacattttac
     4141 cgtgaataat gaactctgtc tgatgaccaa taatagctgt atcagatgga agctcaaaat
     4201 aatctttttg tgaaggcaaa gagccaatgt gaaattgccc ctttggaaaa gatataacgc
     4261 tatttggatg ctgctttgca taagcgaaag cttttcgcaa aagacgattg ttttgctcag
     4321 gggaatttcc tttattgaac ctaatataat aaggtttaac aaaatcttgc tctgaaattc
     4381 catctaatct tagattatct ttcaggttaa tatcgtactg atatcctcct aacgaatagt
     4441 catagcgttt tttaacttgt ttaatagtcg ctaattgacc ttgatacact tttatatgtg
     4501 gctttttagt taatgaagat aatttaacga tatctccttt tttaaacctc ggtttatcag
     4561 gctttttcag gttatgttct ggcaaagaaa cagtgacttt tcctattgga aataccactt
     4621 cataatgata actcactcgg taattttcgg ctatatcttc tacctgttta ttaatagttc
     4681 caatccattg atgatatgga ccactttttt gtacttgaat agcttgtcca atgtgatagc
     4741 gaggctgttg atctttgctt cgttccacta ttccgacagt tgctaatata ttgtgaagtg
     4801 ttgcaaaact attggtccac gcaaggtacg aaacaactaa actcaacaat aatcctagga
     4861 aaattaattc aacaggtcga aataaaccca aaattctctt ttttcttagc atagaccctc
     4921 cctgttgtct taattccctt tcatagcctc tattgtaaca taacgattac gtttttttcc
     4981 aacgagctag agaaattgta atgtcttttc gatgaaaata tgctacaata agttgacaac
     5041 tatcagcctg acggttggta tagaatgaaa ggattataat gactgaaatt tcaattttga
     5101 atgatgttca aaaaattatc gttcttgatt atggtagcca gtacaatcag cttattgcta
     5161 gacgtattcg agagtttggt gttttctccg aactaaaaag ccataaaatc accgctcaag
     5221 aacttcgtga gatcaatccc ataggtatcg ttttatcagg aggtcctaac tctgtttacg
     5281 ctgataacgc ctttggcatt gaccctgaaa tctttgaact agggattccg attcttggta
     5341 tctgttacgg tatgcaatta atcacccata aattaggtgg taaagttgtt cctgctggac
     5401 aagctggtaa tcgtgaatac ggtcagtcaa cccttcatct tcgtgaaacg tcaaaattat
     5461 tttcagacac acctcaagaa caactcgttt tgatgagcca tggtgatgct gttactgaaa
     5521 ttccagaagg tttccacctt gttggagact caaatgactg tccctatgca gctattgaaa
     5581 atactgagaa aaacctctac ggtattcagt tccacccaga agtgagacac tctgtttatg
     5641 gaaatgacat tcttaaaaac tttgctatat caatttgtgg cgcgcgtggt gattggtcaa
     5701 tggataattt tattgacatg gaaattgcta aaattcgtga aactgtaggc gatcgtaaag
     5761 ttcttctagg tctttctggt ggagttgatt cttcagttgt tggtgttcta cttcaaaaag
     5821 ctatcggtga ccaattaact tgtattttcg ttgatcacgg tcttcttcgt aaagacgagg
     5881 gcgatcaagt tatgggaatg cttgggggca aatttggcct aaatattatc cgtgtggatg
     5941 cttcaaaacg tttcttagac cttcttgcag acgttgaaga tcctgagaaa aaacgtaaaa
     6001 ttattggtaa tgaatttgtc tatgtttttg atgatgaagc cagcaaatta aaaggtgttg
     6061 acttccttgc ccaaggaaca ctttatactg atatcattga gtcaggaaca gaaactgctc
     6121 aaaccatcaa atcacatcac aatgtgggtg gtctccccga agacatgcag tttgaattga
     6181 ttgagccctt aaacactctt ttcaaagatg aagttcgagc gcttggaatc gctcttggaa
     6241 tgcctgaaga aattgtttgg cgccaaccat ttccaggtcc tggacttgct atccgtgtca
     6301 tgggagcaat tactgaagaa aaacttgaaa ccgttcgcga atcagacgct atccttcgtg
     6361 aagaaattgc taaggctgga cttgatcgtg acgtgtggca atactttaca gttaacacag
     6421 gtgtccgttc tgtaggcgtc atgggagatg gtcgtactta tgattatacc atcgccattc
     6481 gtgctattac gtctattgat ggtatgacag ctgactttgc tcaacttcct tgggatgtct
     6541 tgaaaaaaat ctcaacacgt atcgtaaatg aagttgacca cgttaaccgt atcgtctacg
     6601 acatcacaag taaaccaccc gcaacagttg aatgggaata acagaataaa cattaaaaga
     6661 ctcgctcatt ttggcgagtc ttttaatgtt tattctttaa ctgttttcgt attgtgtaca
     6721 tggtattagc aatatgataa agtgggctga cagggagatt aaattctcct ataaattctt
     6781 ctattgtcgg agcaaagttg gccttaaatt tggtaagtcc atcatcaagc gagccttcta
     6841 ctcctcccat gttagcccaa ataataccat cttgataagc atcctgaaaa actttaggat
     6901 acagcagata ctgaggataa aacttcttaa aatcatcatt catcccagca taaagcattt
     6961 ccataacatt tccataagaa atagataata tacctgctac aacaacttcc tcaggatatt
     7021 ggtctgtaaa gccttcaaat tcagttatat aacgttctaa agaagctttt tgatcttcta
     7081 atttttttaa acgcttcttt tgatgagctt gggtgcgagt aatatcttgg ttaataagaa
     7141 ttaattgctg gcggtattga tctagttttt gaggaatatt cacttttgct agatgtaagt
     7201 aggccttatc cccataagta gtcatcaact tttgaaagta agcttcgtta cgcaaagaaa
     7261 tatttttacg tttttctgtt aaggagacaa tcttggaaaa tttgtgaagt tctgattgac
     7321 tggcacgata tgtttctaca ccacgctgct tagcatcttt tattaaacgt ctggtatgct
     7381 taggaaattg catctcaagg ttttcttgag tgtaaatatt agcttggaaa cgaggttgga
     7441 tactatctgc aatctccatt gttaaaccag tccaatgaac accagcttct tggagattct
     7501 taatagctgc taaagctaaa ggtttttctt cttcttcctg tcccagtgcg tattgtttta
     7561 acaggatagc tggatcatat ttgataaaga gggccttttt agttttacca taatccttta
     7621 gtgttttcat ggtaaatgcc accaaatcac ggtcaaggta atccatgact ggccctcttg
     7681 gaatataaat aatgcttctt ccaagtggca atagcttaat caaaagtgat aaagaggcaa
     7741 cctgcttttc ctctttatag ataccaattc gctcattctg ccattgattt ttgattttcg
     7801 cccaatcact accttgtaaa acgctaattt gctgatgttc tttaacaaaa gagtcatgct
     7861 cttcttctga aataccgatt tttgcataaa acgttaaatt cgtcatatat tgtccccatc
     7921 tattcgtaat cttagcagat gtacttacta agtatcttca ctgtctttat ctatttattc
     7981 gtaataaatt ttaaaaaaac ataaaaatag ggaaactatt tgaatcactt tttagtaata
     8041 acgtttgtat cttaaatggc ttgttaatca ccaacaattt catgaacaac tgatcatcaa
     8101 aaaataagct attattaaac gactttacaa cattccatta gataaaaaaa agatgtagtc
     8161 acatcttttt ttggttggtg atcagttacc taaaccatca catatctagc agtatttatt
     8221 ctatatcttt actgatttta gta
//
LOCUS       DAUCWU010000069         8199 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUCWU010000069 DAUCWU010000000
VERSION     DAUCWU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.069"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            540..857
                     /locus_tag="VGY24_001378"
     CDS             540..857
                     /locus_tag="VGY24_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ4284356.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFVVENHFDIDDVFWHNIEFHYLASPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
     gene            981..2879
                     /gene="mnmG"
                     /locus_tag="VGY24_001379"
     CDS             981..2879
                     /gene="mnmG"
                     /locus_tag="VGY24_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ4284357.1"
                     /translation="MTHEFTENYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDAEYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            2969..4945
                     /locus_tag="VGY24_001380"
     CDS             2969..4945
                     /locus_tag="VGY24_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ4284358.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            4942..5394
                     /gene="rplI"
                     /locus_tag="VGY24_001381"
     CDS             4942..5394
                     /gene="rplI"
                     /locus_tag="VGY24_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ4284359.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLTITEA"
     gene            5424..6791
                     /gene="dnaB"
                     /locus_tag="VGY24_001382"
     CDS             5424..6791
                     /gene="dnaB"
                     /locus_tag="VGY24_001382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ4284360.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            6808..7080
                     /locus_tag="VGY24_001383"
     CDS             6808..7080
                     /locus_tag="VGY24_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ4284361.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            7810..>8199
                     /gene="rpsD"
                     /locus_tag="VGY24_001384"
     CDS             7810..>8199
                     /gene="rpsD"
                     /locus_tag="VGY24_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ4284362.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIP"
BASE COUNT         2715 a         1289 c         1676 g         2519 t
ORIGIN      
        1 ttgatgatta ggtatttcgt aaaacagatt atttcatgat tattagtacc tattatctaa
       61 tactaaatgt gtaaaaaatt attggaaatt cctagttata cttttatggt tttgttatcg
      121 gcgttcttac ttgtagactt agaaacaacg atatctgtat tttactactt tagaagatat
      181 caaaaaatcg tatgataata ctgatgacat ttttatttct cgcctcgcta gattagacat
      241 agtgatactt ttgtaatcat tttaaaaaca ttacaataga ttgacagagc tgtaaaaaca
      301 ttgacattac tgtaaaggtc tgtgatgaaa tcaatattga caatcactaa tcattttttt
      361 aaaatagata gtaacaatcg gaatgatggt caaagctcat gaaagttgta ggtttttttg
      421 tcctgcaatc ttctagggtt ttggcccttt ttatggttta gctctagaaa tgagatggct
      481 aataggactg ggatttagaa tcactagagg ttagataact ttagtttacg agctagtgct
      541 tgattgttag aaatggtaag aattttttaa ctagagatgc agctgaccaa tattacacca
      601 ttggtggtac aagtttggta ggtgaaaaaa cgcatgaaac tgttttacgt gaaaccttag
      661 aggaagttgg tattcgagct aaggttaatc aactggcttt tgtggttgag aatcattttg
      721 atatcgatga tgttttctgg cataatattg aatttcatta tttggcaagc cccttagaag
      781 accctaaact agaaatgata gagaatgcaa gcgacaggtt tgtgaatgga ttccaatcaa
      841 tcaactggat caaatgaatc ttgttcctga gtttttacaa accgagttgg ctaaatggcc
      901 aggacatatt gtccgcattg aaggctaaac taaagcgtat gattttaaat gaatagactg
      961 aaataaagga ttttaaaaaa atgacacatg aatttacaga aaactatgac gtcattgtca
     1021 taggtgcagg tcatgctggt gttgaagcca gtttggccac tagtcgtatg ggttgtaaaa
     1081 ctttattggc aacaatcaac cttgatatgc ttgcttttat gccgtgtaat ccttctattg
     1141 gaggatctgc caaagggata gtagtacgag aaatcgatgc tcttggcggc gaaatgggta
     1201 aaaatattga taaaacctat atccagatga aaatgcttaa tacaggtaag ggaccagctg
     1261 tacgcgctct ccgagctcaa gcagataaat ccctgtatgc gcgtgaaatg aaacatacgg
     1321 ttgaaaaaca ggctaatttg actcttcgcc aaacgatgat tgatgatatt ctagttgaag
     1381 atggacgagt tgtaggtgtt ttaacagcca caggacaaaa atttgcagct aaggcagttg
     1441 tagtgacaac aggaacagct ctgcgtggcg aaattatttt aggagaactc aaatattctt
     1501 ctgggcctaa taatagtttg gcatcagtaa ccttagcaga taatttaaaa aaactcggct
     1561 tagaaattgg cagattcaaa acaggaacgc ctcctcgtgt caaagcatct tctattaatt
     1621 acgatcaaac ggaaattcag ccaggtgatg acaaacctaa tcatttttca tttatgtcaa
     1681 aagatgctga gtatctcaaa gatcaaattc cctgctggtt gacttatacg aatcaaacta
     1741 gtcatgatat cattaatcag aacctttaca gagcaccgat gttttctggc attgttaaag
     1801 gtgtaggccc tcgttactgc ccatctattg aagataagat tgttcgcttt gctgataaag
     1861 aacgtcatca acttttctta gagccagagg ggcgtgatac agaagaagtg tatgttcaag
     1921 ggttgtcaac gagtcttcca gaagatgtcc aaaaagattt gattcattcg atcaaaggac
     1981 ttgaaaaggc tgaaatgatg cgcactggtt atgctattga atatgatatt gttttacctc
     2041 accagctacg tgccacactt gaaaccaagc taatttcagg actctttacg gctgggcaaa
     2101 ctaatggaac ttctgggtat gaagaggctg ctggccaagg acttattgct ggaatcaatg
     2161 ctgctttgaa agttcaaggt aagccagaac ttattttgaa acggagcgat gcttatattg
     2221 gcgttatgat tgacgatttg gttaccaaag gaaccttgga accttatcga ttgttgacat
     2281 cgcgagctga gtatcgtttg attttacgtc atgacaacgc tgatatgcgt ttgacagaga
     2341 ttggtcgcga cattgggttg gttgatgatg agcgttggaa ggccttcgaa attaagaaaa
     2401 accaatttga taacgaatta aaacgtctga acagtatcaa attaaaacca gtaaaagcaa
     2461 ccaacgatcg tgtgcaggaa cttggtttta aaccattgac ggatgctatg acagctaaag
     2521 aatttatgcg tcgcccagaa attgattatg cgactgcagt ctcttttgtt ggtcctgcgg
     2581 ctgaagattt agatgctaaa atcattgaac ttttagaaac agaaatcaag tacgaaggct
     2641 atattcgcaa agctttagat caggttgcta agatgaaacg catggaagaa aaacgtattc
     2701 ctgcaaatat tgattgggat gctattgatt ctattgcaac tgaggcgcgt cagaaattta
     2761 agaaaattaa tcctgaaaca attggacaag ctagtcgtat ttcaggagtc aatcctgcgg
     2821 acatcagtat cttgatgatt tatttagaag gaaacggaaa agctcatcga aaatattaaa
     2881 tggaaatgaa attgtaatct caaagcaagt ggaagctctg tatttcaggg cttttttatg
     2941 gtataatgtg tgcttaagag gttataagat gaaaaaattt cgttttgaaa ccattcattt
     3001 gattatgatg ggattgattt tatttggact actggcactt tgtgtcagta ttatgcaatc
     3061 aaaaatatta atattattag ctatttttct cgttttgcta tttgttgttg ctcttttatg
     3121 gtatcaaaaa gaagcttatc aattatcaga cttagctcat attgagctct taaatgaaca
     3181 aacagaagat aacctaaaaa cgctacttga taatatgcct gttggagtag ttcaatttga
     3241 tcaagagacc aacgctgtag aatggtataa tccttatgca gaattaattt ttacaactga
     3301 agagggtttt atacaaaaca gtttgattca gcaaattatt accgaaaaac gtcgtgaaga
     3361 tatttctcaa acatttgaag tttctggtaa taaatacact tcttatattg atgtatcatc
     3421 aggaattttt tatttttttg atagctttgt gggaaacagg caattagctg atgctagtat
     3481 gttaagaccg gttgttggca ttatttcggt tgataattat gatgatatca ctgatgattt
     3541 atcagatgca gatacctcaa aaattaattc atttgtagct aacttcattg atgagtttat
     3601 ggagtcaaaa cggatttttt accgtcgtgt taatatggat cgctactatt tctttacaga
     3661 ctttaaaaca ttaaatgatt tgatggataa taaattttct gtcttagaag agtttcgtaa
     3721 agaagctcaa gatgctcaac gccctttaac actcagcata ggaatttctt ttggtgaaga
     3781 aaatcatagc cagattggac aggtagctct tgagaatctt aatattgcgc ttgttcgtgg
     3841 tggtgatcag attgtcattc gtgaaaatgc ggatcatacg aatccaattt atttcggggg
     3901 agggtctgtt tctacagtta aacgttcaag aacacgtacc cgtgctatga tgacagctat
     3961 ttcagatcga atcaagatgg tggacaatgt ttttattgtt ggacatagga aacttgatat
     4021 ggatgcacta ggttcagctg ttggtatgca atttttcgca ggtaacatta ttgaaaatag
     4081 ttttgccgtt tacaatccag atgagatgag tccagatatt gaaagggcta ttgagcgatt
     4141 gcaggctgac ggaaagactc gtctaatcag tgtttctcaa gcaatgggtt tagtgactcc
     4201 aagatcactt ttagtaatgg ttgaccattc taagatatct ttaacacttt ctaaagaatt
     4261 ttatgaacag tttcaaaatg ttattgtagt tgatcatcac agaagagatg atgattttcc
     4321 tgataatgct atcttaactt ttattgaaag tggagcaagt agtgctgcgg agctcgtcac
     4381 tgagttaatt caatttcaaa atgctaaaaa acgtttaaat aagatccaag ccagtgtttt
     4441 aatggcaggt attatgcttg atactaaaaa tttttctacg cgagtgacaa gtcgtacctt
     4501 tgatgtcgct agttacctaa gaagcaaagg aagtgatagt gttgaaattc aaaatatatc
     4561 cgcaacagat tttgaagaat ataagcaaat aaatgaaatt attttacaag gagaacgtct
     4621 tggtgacagt atcattgtag ctgcaggaga aaaaaatcat ctttatagca atgttattgc
     4681 tagtaaagct gcagatacga tactgtcaat ggctcacgtt gaggctagct ttgtattagt
     4741 tgaaacagct tctcataaga ttgctatttc agctagaagt cgtagtaaaa tcaatgttca
     4801 acgtgtgatg gaaaaattag gtggaggagg ccactttaat cttgctgcct gtcagttaac
     4861 ggatatcagt cttccccaag cgaagcactt attattgaaa actattaata tgacaatgaa
     4921 agaaacagga gaagtagaat catgaaagtt atttttttag cagatgtaaa aggaaaaggt
     4981 aaaaaaggag aaatcaaaga ggtcccaaca ggctatgctc aaaatttcct tattaagaaa
     5041 aatttagcta aagaagcaac tagtcaaagc atcggtgaat taaaaggaaa acaaaaagct
     5101 gaagaaaaag ctcaagctga aatattagca gaagctcaag ctgtcaaggc tgttttggat
     5161 gaggacaaaa cacgtgttca atttcaagaa aaagttggtc cagatggacg tacatttggt
     5221 tccatcacag ctaaaaagat ttcagaagaa ttgcaaaagc aatttggtgt aaaagttgat
     5281 aaacgtcata ttgttttaga tcatcctatc cgcgccattg gtctaattga agttcctgtg
     5341 aaattacata aggaagtaac agctgagatt aaattgacta tcacagaagc ataatataaa
     5401 aaacatcaca agatttggag gttatgaggt tgcctgaagt agctgaatta cgagttcaac
     5461 cccaagattt actagcagag caatctgttc ttgggtcaat ctttatctca cctgataagc
     5521 tgattgcagt gagagaattt atcagtccag acgattttta taagtacgct cataaaatta
     5581 tctttcgggc aatgattacc ctcagcgatc gtaatgatgc cattgatgca accactataa
     5641 gaacaatcct agatgatcaa gatgatctgc aaagtattgg tggcttatcc tatattgttg
     5701 aactagttaa tagtgtccca actagtgcta atgcagaata ttatgctaaa attgtagctg
     5761 agaaagctat gttgcgtgat attattgcta ggttgacaga atctgtcaac ctagcttatg
     5821 atgaaatttt aaaaccagaa gaggttatcg ctggagttga gagagcttta attgaactca
     5881 atgaacatag taatcgtagt gggtttcgca aaatttcaga tgtgctaaaa gttaattacg
     5941 aggctttaga agcacgttct aagcagactt caaatgttac aggtttacca actggtttta
     6001 gagaccttga caagattaca acaggtttac acccagatca attagttatt ttagctgctc
     6061 ggccagcagt ggggaagact gcctttgttc ttaatattgc gcaaaatgtg gggactaagc
     6121 aaaaaaagac tgttgctatt ttttctttgg aaatgggtgc tgaaagttta gtagatcgta
     6181 tgcttgcagc agaaggaatg gttgattcgc acagtttaag aacagggcaa ctcacagatc
     6241 aggattggaa taatgtaaca attgctcagg gagctttggc agaagcaccg atttatattg
     6301 acgatacgcc cgggattaaa attactgaaa tccgcgcaag atcacggaaa ttgtctcaag
     6361 aagtggatgg tggtttaggt cttatcgtaa ttgactactt acagttgatt acaggaacta
     6421 aacccgaaaa tcgtcagcaa gaggtttcag atatttcaag acagcttaaa atcctagcta
     6481 aagaattgaa agtaccagtt attgccctaa gtcagctttc tcgtggcgtt gagcaaaggc
     6541 aagataaacg accagtttta tcagatattc gtgaatcagg atctattgag caggatgccg
     6601 atattgtagc cttcttatac cgggacgatt attaccgtaa agaaggtgat gatgctgaag
     6661 aagctgttga agataacaca attgaagtta tcctcgagaa aaatagagct ggggcgcgtg
     6721 gaacagtcaa actgatgttt caaaaagaat acaacaaatt ctcaagtata gcccagtttg
     6781 aagaaagata agaaagagag acaaaaaatg agcgatgcat ttacagatgt tgcaaaaatg
     6841 aaaaaaatta aagaggacat aagagcccat gaaggacagt tagtagaatt aacccttgag
     6901 aatggacgga aacgggaaaa aaataaaatt ggtaggctta ttgaggttta cagttcgctc
     6961 tttatcattg agtatagtga tagctctgat actccaggag caattgataa ttcttatgtc
     7021 gaatcataca cctattcaga tattttgaca gaaaaaacat taattcgtta tttagattga
     7081 tttttgcctc atagaggctt tttctttaca ataaattgat ataattatta gcgatagaag
     7141 attattctgc ctgtattctt tttattttga aaaaattcct ataaaagata atatttttag
     7201 gttagattga aggaaaaaag aggtcttcac catggttaag caaatgatct tagtactaag
     7261 gttaaaagat tatcgtgaaa taaagttttg acacaagtgt aattaataga ggaacttgaa
     7321 gtatccgata aaactatctt gaagtgaaaa aacgagtaga cctcccctga taaatacaat
     7381 atactagcca tttcagagca attaagagtt tccagtgaag tgcttttgat agagagctaa
     7441 gaaaaaaaca gtcaaggttt aagaactgca aattagcatg taaattattt tttgttaatg
     7501 acagttgtct ttattaccat aactataact tcttataata tagtcttctt aattcatcag
     7561 tggtggttcg tcccatatct gaccttaaaa tattaaactg atcatcagat gatagataga
     7621 ctgactttac aaaacaactt tcttatggta aactgttctt tgtgcgaaat aacagcagaa
     7681 aaatatgaag ctcgtcaaca ggtggttatc tattcaagta acctttgctg tttaggcgaa
     7741 aaccccctgc acgaatcagg attttttaaa ccgttatttc ctcaaaaaat ttagaggagg
     7801 acatgtaata tgtcacgtta tactggtcca tcatggaaac aatcacgtcg ccttggttta
     7861 tcacttacag gcacaggtaa agaattggca cgtcgtaact acgttcctgg tcaacacggt
     7921 ccaaacaacc gtagcaaact ctcagaatat ggtttgcagt tagctgagaa acaaaaactt
     7981 cgtttctcat acggtttagg tgaaaaacaa ttccgtaact tgttcgttca agctacaaaa
     8041 atcaaagaag gaactcttgg tttcaacttt atggttcttt tagaacgtcg tcttgacaac
     8101 gttgtttacc gtctaggttt agcaactact cgtcgtcaag cacgtcaatt cgttaaccac
     8161 ggtcacatcc ttgttgacgg taaacgcgtt gatatccct
//
LOCUS       DAUCWU010000070         7783 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUCWU010000070 DAUCWU010000000
VERSION     DAUCWU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.070"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..546)
                     /gene="speG"
                     /locus_tag="VGY24_001385"
                     /pseudo
     CDS             complement(1..546)
                     /gene="speG"
                     /locus_tag="VGY24_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
     gene            complement(648..746)
                     /gene="ffs"
                     /locus_tag="VGY24_001386"
     ncRNA           complement(648..746)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGY24_001386"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            946..2178
                     /locus_tag="VGY24_001387"
     CDS             946..2178
                     /locus_tag="VGY24_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ4284363.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNNSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2792..3307)
                     /locus_tag="VGY24_001388"
     CDS             complement(2792..3307)
                     /locus_tag="VGY24_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ4284364.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(3307..4086)
                     /locus_tag="VGY24_001389"
     CDS             complement(3307..4086)
                     /locus_tag="VGY24_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ4284365.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PDGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(4226..4765)
                     /locus_tag="VGY24_001390"
     CDS             complement(4226..4765)
                     /locus_tag="VGY24_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ4284366.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(4769..5080)
                     /locus_tag="VGY24_001391"
     CDS             complement(4769..5080)
                     /locus_tag="VGY24_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284367.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(5297..6439)
                     /gene="tgt"
                     /locus_tag="VGY24_001392"
     CDS             complement(5297..6439)
                     /gene="tgt"
                     /locus_tag="VGY24_001392"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ4284368.1"
                     /translation="MTDYPIKYRLIKTDKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDDLIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDCTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6658..7521
                     /locus_tag="VGY24_001393"
     CDS             6658..7521
                     /locus_tag="VGY24_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ4284369.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLILAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFSIYINRYYAYSLSEYLLYDEVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
BASE COUNT         2440 a         1594 c         1375 g         2374 t
ORIGIN      
        1 aaggctcccc gatgtataac gcgattccga atcatagata ttgtattttt ccattagaaa
       61 ttttcttatt ttaaaatcaa attcctgtaa agtaaactgt ttctttttta tgcgaacagc
      121 ctcagagggc aaataagttt ctttcccatc ttgatttaaa attcctacaa actataatga
      181 tttatttcct tgattcgaat gttttacaat accaccataa atattatcta cataaggtgg
      241 agaaaaatta taagggagac caaaaacatc gatcttatcc ccttttataa actgatcagt
      301 aatgcctaaa gatacaatag aatcaacata aagaatacat tccgatcttt tttgtttagt
      361 attaatatgt atgctatttg tagtaacaaa tgcaatttct acattttcat aatcgcatgg
      421 ggtaatatta taggcaaacc ttaaacttct ttttaaatct tttaaatttt catctgcata
      481 agctaattga cttccatagc taaaaacaca tgataatatg ataattgtca aaatgtttgt
      541 tttcatttaa aactttttat gtcctgtttt atacctctta acctaaataa aaatatcaat
      601 cggtttcaac aactgatgac actatagaag gaggaggtta ctttagtaaa gtcaacaaaa
      661 aaagagcaca cacccgaaaa tcgcttaggg ctgctggatt cctcccctga cccacttcac
      721 gcacaagtgt tgctccgcct attattatat catattacgg acagtttgca agttttccaa
      781 ctaagtaaaa tcactttcaa gtgagcatag cttcattttg ggacaagatg gactgaatac
      841 ctagaaaaat catgaccctt tccaaacacg attttgcttt ttgaaaatct ttccctctcc
      901 ccattccagc tattctctat gttataataa gattattaga ttagaatggg agttatgatg
      961 aaacaaaaga ttaaaatctt aacagtaatt ggccttatga cagttggtat gtctgcgtgt
     1021 cacaacaata gcaaaccttc taataccgat tctgtttttt ctttaaccgg aaaaaagcgc
     1081 caacaaatcg tcaaacaagt aagacaacgg tattatttcc aacaattatc caaaactgaa
     1141 caagaaaatt acctcacttt atacgatagt ctagcacaat ttcgtgaaat catcagtttg
     1201 acgccggctt ctaaaaaaag cttaattaaa acgattgacg cctttgtgat ggataatccc
     1261 gaattttact ggattacatc tgccgattat cgatttgagt tttctgatca aacggtcttt
     1321 gtcacctttc ctatcccaga agatgccaag aacgtttatc aagacttgca agcaatcggt
     1381 aatgatatcg tcgcaaacac gccatcaaag gatcgctatg agcaggtcaa atatttttat
     1441 gaggtcatca ttcgagacac agactacaat aaaaaagcct ttgaagccta tcaatcaggc
     1501 agtcaagccc aggtcgcttc taatcaagac attaaaagtg tttttattga tcatttatct
     1561 gtttgtaatg gctatgccca agcctttcag tttctttgtc aaaaagctgg cattccagtc
     1621 gcctatatcc gtggaactgg tacatctcag caacctcagc aatcttttgc acatgcatgg
     1681 aatgctgttc aaattaataa cacttattat ggcgttgatg tcacttgggg tgaccctgtt
     1741 tttgataacc atctctcaca tcaaaagcaa ggaactatca attatagttt tctatgccta
     1801 ccagattatc taatggcctt atcacatcag ccaagcaaag acattgcttt taatactaaa
     1861 gaacgttttg aaaatgtttg gactatacct tcttgtacag atgattcttt gctttattct
     1921 aaacgtcatc aaagctatat ctcaacgttt gacagtgacg ccatccttgc aagccttgaa
     1981 aatcagttgt taaatagaca agagccactt tctctacaat ttgctcatca agatgattat
     2041 caacagatgg taactgattt aacaacgaat caaacaggtt atcataacct tttcaatcag
     2101 tattggaata attatacggg ttttacctat ggactcttac cagagactct tagtatttca
     2161 tttgctagta ggaactaggt ttagacacat gaaacacaaa gttaagatga ttatctttgt
     2221 caaacattac aagaaattga tatactaaca caattgaagg cgattattat tctaaccgtc
     2281 ttcaatagat ataacctttt cagattgagc acatctaatg cttgtaacat tagcaacagt
     2341 gatgagctct tttttaatgc tttagcaaaa tatatcagct aagacaaaag attaaataac
     2401 tcaagccatg cctataagtc acttgttttt aatccctgtg tcagtgtcaa tctacgccac
     2461 agataactga cagctctgag ccttagaagt ctgagcttga taagtttact aaaaacaaaa
     2521 atcaggtgag ccattactat ctgtttttag atagtaaatc tttttaagaa tgactaactc
     2581 atctagtcat cacgtgtttt atttttcgat tgttttcacc tcgattgggt ctgaaaaaga
     2641 gcgcttctag tacttggaaa cgcccttttg tatattctat cttcgtatgt tgaatctaac
     2701 aaccattgga aaaatggtct aacgtttcac tcattatttt gacaggctac ccttcaccaa
     2761 aaatgcatgt gataacaaac tccttaaaac tctagtcaaa gggatctgac tgttccttga
     2821 tcaaatgctt agctattttt ttgcgctctc gcccttggcg gaaaaaggtt tgcatgatgt
     2881 tagcacagtc tgctgctagc aggcctcgtt caacttgcac gcggtggttt aagcgctcat
     2941 ctgtcaaaat ctggtaaagg ctgtctgctc caccaaattt ttgattgctg gctccataaa
     3001 taacatgcgg aatccttgct agcccaatgg caccactaca catcacacaa ggctcgatgg
     3061 tcacaaagag tgtggtgtct aataaacgcc agttgccttc atgagcgtta gcctcgttaa
     3121 tagccatcat ttcagcgtgc ataatggctt ggttactctc ttcacgcgca ttgtgcccac
     3181 gaccaataat ctcaccatcc ttgacaatga cacagccaat ggggatttca gctttctgta
     3241 aggatttttc ggcttctttt aaagcctctt gcatgaaata agtttgctct tctaaactat
     3301 atggcatcaa gaaagactcc ccaagttcca agatttgtgt ggtaaggcaa ggtcaacagg
     3361 aatattacct gcataggcct gtgtttcttg cctcaacact tctaaatggt ttttggaatt
     3421 aataccatct ggaggaaccg gtggcatgtg ggtcaagaca agcttcttaa cacctgcctg
     3481 gctagccatt agtcctgctt ccttactggt caaatgggcc atatgatttt cattgccttc
     3541 gtataagtaa acatccgcta agaaaaggtc tgcttctttt gcgaagtctg ccaatccttc
     3601 aaaataacca ctgtctccag taaagacaag tacctgacca gtcgctcttt ctacaattcg
     3661 aaaggcataa cacacaactg gatgaactgt tttaagaaag gtaatgtcaa aaggaccaat
     3721 ctgttcaact ccagtaacat cataggctct accttctgaa acatcaggaa tgcttaactt
     3781 agcaaattcg tgtcggtcct catcatgccc ataaatgggc aatactttag gctgccacag
     3841 gtgtttagga taaagttgaa aataatggcg caacacgcct aaatcggcca cgtggtctgg
     3901 atggtaatgg ctaataatca ctgcatccaa atcgagagga ctgatttctt tttccagctc
     3961 attgagcgct cgactaccag catccatcaa taactgaaag ccgtcatgtc ctgtgaccaa
     4021 ataagaggtt gtgcctgcat cttgataggg ataggctccc caagacccta aggtggttaa
     4081 tttcatctca aacccttctt tcttgcttta actcttatta taacaaaaat tagtgtcaaa
     4141 gaggattgag acccaccaaa aacaggtcct agcagataaa tctgccaaga cctgttcaac
     4201 acaacttaac tatttgatga ttagattact gttttgtatt caaaaaacta tctttgtatt
     4261 taacagcgat aatggtcgca atgattgctt tgatggtatc tcctggaata aagaccaagt
     4321 ttgacaaaag cgatgtcaca agagacatgc cagtgtaggc tgccaaccag atagcaccca
     4381 gaacatcaac cagtacaact cctgagatta aaagagctag gaatgctaat ggcgtgcttt
     4441 ttgagaacca gttgcggtaa agtaatgaaa agacaatagg cacaagaagg taggcaataa
     4501 catagccagc agatggcccc atcaatactg gaatggttgt tcttgagcct gagaaaacag
     4561 gtaagaaaag gccaatcact aaaaataaga aaacagataa ggttcccttt ttaccaccca
     4621 gcatcaatcc agccaacatg acacctaagt tttgtaaaac aatagggact gggataaagc
     4681 ctagcgggat agcaggaata aagcctaaaa taataatcaa agtggtcatc atagccacct
     4741 taacaagttc tttggttgta aacatcagtt acgactcctt taaaaaatag atatccttgt
     4801 gcctatgaca ggctggatta aagggacttt gacaataagg acagcatcca caaccatact
     4861 cagctaggct caagagcttt cgacaatgcc cacaaataac aggagatgtt tcttgatgtc
     4921 ctgttggcac aaaggcatga tctgttaact ggtcatggca atggtaacaa gcataataag
     4981 tctgacaaaa agcacatttt agcccaacaa tatcaagcgg ggtatgataa tgtaggcacc
     5041 gatactcttg gtcaaggtca attccaaaac agtccgtcat atctcatcct cttgtaacag
     5101 tttaatcaaa agcttatgat ttgtcaacta tatttttaca aaagggtaac tgttttctga
     5161 aaaagaacac gtaaaacaag aacttccctt taaagagtga cctatcagtt gaaagcaatg
     5221 ggacatccgt ctatacaaaa aaactgaaac caaagacgat agatcgccct cagttccagg
     5281 atcatagcat ttttaattag aaattacggt ttgatttatt ataaccatat cgttcaagaa
     5341 aatcttggcg gaattcaagt aagttatcgt ccataatagc ctgacgtacc tttttcatca
     5401 agttaaccaa gaaataaagg ttgtggtagc ttgtcaaacg aataccaaaa gtttcgtccg
     5461 cttttagcaa gtgacgaatg taggcacggc tatagttttg acaagtgtag cagtcacagt
     5521 catgatcaag tggggtacaa tcttcagcga atttggcatt tttaaccaca agacgcccct
     5581 cactagtcat gcaggtccca ttacgagcga tacgcgttgg caagacacag tcaaacatat
     5641 cgacaccacg aatcacacca tcaatcaagc tatctggtgc tcccactccc atcaggtaac
     5701 gaggtttatt ttctggtaag aggggagtcg taaagtcaag gacggcattc atttcttcat
     5761 ggctctcccc aacagctaaa ccaccaatag aataacctgg gaaatccata gccaccaaat
     5821 ctgccgcaga ctgacgacga aggtcctcaa atccagctcc ttgcacaata ccaaataatc
     5881 cttggtcatg aggacgacga tgggctttta ggccacgttc tgcccaacgg ctagtacgtt
     5941 caatggattt tttcacgtaa tcataaggtt ggtaaaattg cgggcactcg tcaaaactca
     6001 tcatgatgtc tgagccaagg ttattctgaa tggaaatggc tttttcgggt gatagaaaca
     6061 tttttgagcc attcaagtga tttttgaaag tcactccttc ttcagtgatg tttcgtgaat
     6121 cggctaaaga gtaaacttga aaaccacccg aatcggttaa gattggctga tcccaattca
     6181 tgaacttgtg cagaccacct gcacgagcaa tcaaatcatc tcctggacgc aaccagaggt
     6241 gataagtgtt tgataaaata atccctgacc caatagcttt taactcttct ggagactgtg
     6301 ttttaacagt agcctgagtt cccactggca taaacattgg tgtcggaaag gtcccgtgag
     6361 gggtgataat ttcgcccaag cgtgcaccgg tgtgcttgtc tgttttgata aggcgatact
     6421 taatcggata atctgtcatg tgctttcttc cttgctatca agataaacag tcttgagctt
     6481 ttttgatctg cctgagtttc catcataatg aagggcttca agatgacatg ttgttctagc
     6541 gcctatcatt ttatcaaaaa atccataatt tgtcatgctt tcccaaaaaa gtatagtcaa
     6601 aataatggtt agctgtctca ttttgtttta tggtataatg aaataaagga gtctgttatg
     6661 tcaattaaag caatcaaagg ccaggctaga gacacgttaa aaaatctctc tggcaagtat
     6721 ttactatttt taattccaac ccttctcttt atgtttcatt tcggtattga gattcatcaa
     6781 ggttacgtcc tatctagtgg tattgaagtc tcgctagcgg ctagttattt cccattacta
     6841 ctaggactta tccttagcct tttcatttta tcagcatcct ttacgatgat tgatgttgtt
     6901 aggcattttc gtcagaaagt ttcttttgcc gaatcaacaa cagccttctc aaaagaattc
     6961 tttgggaacc tgctgatttt agcgatcact aagtggctat tctttctcat ctggtcacta
     7021 atttggtttt ttggcctctt tattttcctt tccggacttt cagcgttttt ggtcaatgct
     7081 aaatcaggta gctcaacagt catttcactg atctttttac tatttggagc agttttgtcc
     7141 ttaattggtt ttagtattta tatcaaccgt tattacgctt atagtctatc agaatacctt
     7201 ctctacgatg aggttaaaga ggggacttat ctaggagcta tagccgtcat tgaaacaagt
     7261 gtagctatga tgaaaggtta taaatggaag ctttttttcc tacaacttag cttcaccggt
     7321 tggtttctac tgaatattgt tacttttggg ttacttaata tctatctttt accatatttt
     7381 acaacagcca atgtcatttt ttatgatcaa ctcaagaaac gtttcaaaga caaagatgac
     7441 ccaattgaag gagaacattt aagccttcat cagcttaata aaaagcctac tcctatgatg
     7501 tatgacgatc aaaaagacta aataaatcct ccatccataa aaatagccct tgaactgcac
     7561 cccaaaagtt agacacaaaa tctaacgatt ggggtgctat tttttataaa atttaatcaa
     7621 gaaacgaaag ttaagattta tgagttacgg caaatgggag agtccattaa atccatatca
     7681 aaaaagtttg atatggcaga atccgatctc aaatatatga ttcacttgat tgataggtat
     7741 ggggtaacca ttgttcaaaa atgtaagaat cattattatt ctc
//
LOCUS       DAUCWU010000071         7626 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUCWU010000071 DAUCWU010000000
VERSION     DAUCWU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.071"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            196..1284
                     /locus_tag="VGY24_001394"
     CDS             196..1284
                     /locus_tag="VGY24_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ4284370.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQSARE
                     TLINLQAELDKPKSSMTFGALTSQWLKEYEKTVQGSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRK
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILLELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
     gene            1647..2267
                     /locus_tag="VGY24_001395"
     CDS             1647..2267
                     /locus_tag="VGY24_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ4284371.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDREPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(2523..4787)
                     /gene="glgP"
                     /locus_tag="VGY24_001396"
     CDS             complement(2523..4787)
                     /gene="glgP"
                     /locus_tag="VGY24_001396"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ4284372.1"
                     /translation="MTRFTEYVEIKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLVIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(4822..6315)
                     /gene="malQ"
                     /locus_tag="VGY24_001397"
     CDS             complement(4822..6315)
                     /gene="malQ"
                     /locus_tag="VGY24_001397"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ4284373.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(6429..7448)
                     /locus_tag="VGY24_001398"
     CDS             complement(6429..7448)
                     /locus_tag="VGY24_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ4284374.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIIDSNEQNLSQTIFVPFSLKQRESV
                     RDISH"
BASE COUNT         2492 a         1421 c         1267 g         2446 t
ORIGIN      
        1 ttagtacagt tgagtttaat gatgtactat cttctatggg gataaattta actgaactgc
       61 tcaaaatcaa ataaaaaaaa gccctacgct caaattttgg tcgaggagag cgtaaggcga
      121 attccagtat agtaaaatcc ttttttaaga gggcttttac tatacctatt ttatcaaata
      181 gagggatagt aggcaatgaa aattaaatca tataaaaagg aaaatggcga aactgcttat
      241 aaatttcttt tgtatgccgg ttatgttaac ggaaagagaa aatatattag gcgagaaggt
      301 ttcaagacta agcaatcggc acgtgaaacc ttaattaatt tacaggctga acttgataaa
      361 cctaaatcaa gtatgacatt tggagcattg acaagtcaat ggctaaaaga atatgaaaaa
      421 actgttcagg gcagtaccta cttaaaaacc gaaagaaata tcaataaaca tattttgcca
      481 aaacttgata aagtgaagat tggagacatc aatccactac ttatccagcg gcttactgaa
      541 gaatggtgca acgatttaaa atatggagga aaaattcttg ggcttgttag gaatatctta
      601 aatctagctg ttagatacgg atatatcaat aacaatccag ctttgccaat tacacctcca
      661 aaaataaaaa ggaaaagaaa aatgaataat aatttttata cacttgatca acttaaacaa
      721 ttccttgaac tagttgaaaa aactgacaac attgaaaaaa tagccttgtt tagattatta
      781 gcatttactg ggatacgaaa aggggagctt ctggcactaa cttgggatga tttgaatggc
      841 aatactctat caattaataa agctgtcaca cgtactcaag ttggactaga aatagatgtt
      901 acgaagacaa aatcgagcga tagattaatc agcttagatg atgaaacttt ggaaatttta
      961 ctagaacttc atgaaacttt tcctacttct actcttatgt ttcaatctga atcaggtgga
     1021 attatgacgc caagtttacc acgaaaatgg ctattgcaaa ttatcaaagg gacagactta
     1081 ccacaaatca caattcatgg tttcaggcac actcatgcaa gcttactttt cgaatcaggt
     1141 ctatccttga aacaggtgca acatagatta gggcatggag atttacagac aactatgaac
     1201 gtatatactc acatcacgca atcggcaatt gatgacattg gaactaaatt caatcaattt
     1261 gttactaaca agcaactaga ttgacaacta attctcaaca aacgttaatt taacaacatt
     1321 caagtaactc ccaccagctc catcaatgct taccgtaagt aatcataact tactaaaacc
     1381 ttgttacatc aaggtttttt ctttttgtct tgttcatgag tttccgttat ccctatagcc
     1441 ataccatcac gcttaccgat actccatcaa taccactcaa aaccttgtca tatcagagct
     1501 ttttgaccat ttttttcatg aatatctaaa aaaaagaatc aaaatagtac taaattcccc
     1561 atagcgcatg cgctatgggg aatcatagct atatcttgtt tataatttat gatataatac
     1621 agcaaaaata gtttaggaaa taacatatga caaaaaaaca cttactaaca cttcttctca
     1681 tctctttttt tactagcttt ttggtagctt gttcaacaac gaaagataga gagcctcaac
     1741 cgtctgattc agaaatcatt actccccgac tacaccaagc cgctcatcaa gataaacgcg
     1801 ctaactttga aaaaattaaa cttgcgactg ttgattcctc atttacagga gggacaagcc
     1861 ttgaagaact tatttcactc tttggagagc ctagccaaca tgatccaaaa acagcaggcg
     1921 aagtaacaat cgacgcttat acttggcagt ttgatcaagt tactctcact gttaatcttt
     1981 atcaaaatag tagtattgtt aaaaccatct ctaattttac ctttgcaaga gagttaggct
     2041 tatcgcaaaa ggaataccaa caattacaaa aaggaatgtc ttatgaagac gttaaaaaga
     2101 tcttaacaga acctgataat tatagccaag cgtcatctag tgatcatcaa actttgcaag
     2161 cgatttgggt tagtggctta aagacagata caagcggagc taatatttct ctcgtttttg
     2221 aaaataatca gttaacagaa atgtctcagg taggacttga agaataaaaa atgcgtaata
     2281 tattttgcta aaaagattga tttcgatgta caccgaaatc aatcttttta tatcatttta
     2341 ataaaaaact ttagtgtaat ttcttataat tgccttcata gatcgataaa cagacttgtt
     2401 ttacgcttaa tgtaataatt aatctaagct gaagaaaaat cataagacag cataaactac
     2461 tgtcttatga tttagtttaa gcatgttacg gtaccgctac cccacattgc aattattatt
     2521 ttttatcgtg aatgccaaat atcttcgtta tactgctcaa tggtacggtc agatgagaag
     2581 aatccagctt ttgcgatgtt atggaccact tttgtcatcc ataaatcttg gtcttcatag
     2641 tctgctaaca ttttttcttt cacttcaatg tactcttcaa ggtcaatcaa agtcatgaac
     2701 cagtcttttg aaatcaattc tttataaaga cgatcaagac gttcttcgtt gccaaatgct
     2761 agcaattctg gactgataat aaagttcact gctgctttaa tagcaggatg gttatcatag
     2821 taatcttttg ctacataaga agcagtcgca taaaggttga tgatggtgtc agaatcttta
     2881 ccaaaggtgt agatattctc catgcctgca agctcagcga tttctacgtt agcaccgtcc
     2941 attgtaccaa gtgttaaagc accgttaagc atgaatttca tattaccagt tccagaagct
     3001 tctttggatg ctagtgaaat ttgctcagaa atatcagttg ctggaatcaa gtgctctgct
     3061 actgtcacgt tgtaattttc aactagatgc acattaaggt atgggcttac ttcagggtca
     3121 ttgttaatca attcagacaa gcaaaggatc aagtgaatga tgtcttgagc aatgatgtaa
     3181 gcaggcgctg ctttacctcc aaagataaca gtgattttac gttttggaag gttgcctttt
     3241 ttaatttcca aatatttgtg aatcacgtaa agagcattca tctgttgacg tttgtactcg
     3301 tggaaacgtt tgatttgggt atcaataata gaatgttcat caagctcaat gtctttattg
     3361 tctttaaggt aacgtttaag agctaatttg ttgttgaatt tgatttcagc caatttagca
     3421 tgaacagctt tgtcatctgc aaaggccatc aatttttcta gttttgttgc gtcagtcaag
     3481 tactcatcgc caataagttc tttaatgtaa tcagccaagt cttggttagc aaattctagc
     3541 caacgacgga aagtaatccc gttggttttg ttgttgaatt tttctgggta aaggtcatag
     3601 aaagctttta attcgctgtt tttcaagatt tctgtgtgga gtgctgctac cccattgaca
     3661 cttgttgcaa aatgaatatc catatgggcc atgtgcacac gacctgattc gtcaataatt
     3721 tgaacagctg gatcagacac ttctgaacga acaagagcat ccaatttttc aatgatggtt
     3781 accaagtgtg gcactacttc gtttaagtaa gcagttggcc atttttcaag ggcttctgca
     3841 agaatagtgt ggttagtgta accaaccata tttttcacaa cagctaccgc ttcatcaaag
     3901 tcaaagccat gtttttcagt caagagacga attaattctg ggatgaccat tgatggatgc
     3961 gtgtcattga tttgcacgta agcgtagtct gcaaggtcat gaaggtttga accacgttcg
     4021 atagcttcat caatgactaa ttgcgctgca tttgacacca taaagtactg ttggtaaata
     4081 cggagcaatt ccccattttt gtcagaatcg tctgggtaga ggaacaaggt caagttttta
     4141 gcaatttgcg ttttatcaaa tgaaataccg tctttgatta acccgtaatc aacgccctcg
     4201 atatcaaaca agttaaggta gtttttagtg tcacgtttgt aacccaaaac atcaatacga
     4261 tcaagacgag attttaaggt aaagtttttg aagggcacat cgtaagaaat gtctgttgga
     4321 accaaccatg agtcatcttc aatccagtag tttggctcag cttcttgctc attgtgtttg
     4381 aagacttgct taaacaaccc acaatgatag tttaaaccaa ccccttcacc attaatacca
     4441 agagatgaaa tagagtcaat aaaacatgaa gcaaggcgtc ctaaaccacc attacctagt
     4501 gatggttcta attcaacatc ttcgacttct gcgatagatt tgccagcagc tgccaattct
     4561 tctttgatat ctttataaat tcctaggtta atcaggttgt ttgatagtaa tttaccaatc
     4621 aaaaattccg ctgagatata gtaaactttg cgtttggcag tatttttagc tttgtggcta
     4681 gcttcttctt tgacaaagtt taataatgat agataaatct cttcattgct agcctgcgta
     4741 agtgattttc ctagtttaat ttcaacatat tctgtaaagc gtgtcatgtc ttcctctttt
     4801 caataatgta acatttaaat attatcttgc gcgaccataa agatctgtta aataaataag
     4861 gtaatctttg tgatcttggt tcaattcacc gtcaagcata cgccattgcc aattacctcc
     4921 aattgtattt ggcatattca tacgactctt accatcttta tctaataaat cttggatgca
     4981 aagaatggct gtatcacaaa cagaagcaaa gattgttctt agcatggctt ttgtaatact
     5041 ttcatctgca tgtttgtgaa gatagttatt tacaaagtgt acttgttctt cactaagatt
     5101 gtcataccat ccgttaacca cctcgttatc atgagtccct gtgtaagcga cacaattacg
     5161 gtcatagtaa tgtggtaaat ctatactttg actcgttata tcaaataaac caaattctaa
     5221 tatcttcatg ccaggaaagc ctgttgatgc taatagctgc tctgcttttt catcaatata
     5281 accaaggttt tctgcgatga taggaagctc tcctaaggcc tcacgaaccg cagaaaataa
     5341 cgctatacct ggggctgagg cccaatggcc atttctagca gttttatcac cagcaggaat
     5401 ttcccaaaaa tctgagaagc ctttaaaatg atcgattctt agctgatcat atagtttgaa
     5461 actttcttga atacgataaa tccaccatgc aaaattagac ttttcatgag cagaccaatt
     5521 gtaggttgga tttccccaca gctgaccatc ttcactaaag ccatctgctg gtacacctgc
     5581 aataaacaat ggctttttat cactatctac tttaaatagc tcaggcatcg tccaaacttc
     5641 tacactgtcc gcagaaacat aaataggcat atcaccaata atttctatcc ctttatggtt
     5701 agcgtaagtt ttcaaggctg accattgttg gtaaaagaag tattgacaga ctttatgata
     5761 ggtaatcttt tttgcaagca actcacggta attattaagt gagtcttctt ggcgtttaat
     5821 gacagtttcg tcgtcccaat cttgtaaggc tttattgtta aaatattctt ttaaagccat
     5881 aaattcagca aaatcagtta accagctaga agctgtttca aaagcttcta atttacacac
     5941 attttcttgt tcagcaacaa aagctctcac tgctttttct aaaagtggac gcctaacttg
     6001 aaaaagctga gcatagtcta ctgcttcagg atttgtgccg aatgtaatat cacacaaatc
     6061 tgctgcttct aaaagttcat catctactag caattcaaaa tcaatgaaat gtgtgttccc
     6121 agcaatagct gaaaatgact gataaggaga atctccaaaa ctcgtcgttg ttaaaggcag
     6181 aatttgccaa taggtttgtt tcgtctctgc tagaaaatca acaaattcaa aagctgattt
     6241 tccaaaagtg cctatcccaa atttcccagg cagggaacta atatgcatta agataccgct
     6301 tgcacgtttg ttcatgattt ttctccttgt gaaatctatt catgatgaga ttataacgca
     6361 agcgcttgcg taagtcaacc agtttcctaa aaaaagtaac attccttaaa aatgctactt
     6421 ttttgttctt aatgactaat atcacggacg ctttctcttt gttttaaact aaaaggaaca
     6481 aaaatagtct gactaagatt ctgttcattg gaatcgataa tatcaagtaa ttgcttaaaa
     6541 gacatttttc caagattttc aacatttata tcaaatgtcg ttaaataagg gtgaattaat
     6601 ttagcgtaat tagaattatt aaaagtgata attgacatat catctggcac ttttatatca
     6661 taaaaagata ataactgtac catacgaata gccagaacat caccaacaac aaccaaggca
     6721 gttgctttaa aggaagatat agtttccatc aatatttcta cgctaacagg atccttacga
     6781 tcaaataaca gcattggctt cgtttttaaa gatcgcttaa aacaaccctt caaatagcca
     6841 agataacgct cagaagttac ttctgatagt aaatcatctg ttataaataa tatgttgtga
     6901 tggccttttt gataaaggtg ttctactgct gttttagcca ttagctgatt atcattatca
     6961 atataagtgg ttttattagt gtcaccttgt ggagcaccta caatgacaaa tggtaagtta
     7021 tttgtcatca aatatttttt gacaggatca tcttgatcag aataaagaat aataaaacca
     7081 tcaactcgtt tttgaagatg catcagtttc acttgctcta tcaggtcatc aagagatata
     7141 cctgtagcaa tcgacacagt aaaatgagat tctttagctt cgttagtaat agtcgacaaa
     7201 atttccataa agaaaggttc acttaatcta tcgctagttg taataggagg aaaaacaagt
     7261 cctatattgt gtgtcaaacc actcgctaga atctgtgctg cgacatttgg aacatatccc
     7321 aaatctgcca tcgcttttct aaccttctct tttgttttca tagaaataga tcgattatct
     7381 tttaataccc tactcaccgt cgaagggtta acacctgcct tttgagcgac atcttttatt
     7441 gttaccatat taacctcgtt actatattta ccaaagataa accttatcat gggtttgtca
     7501 tgtatttcgt ttccattata caattatctt tcaaaaaatc aacttcaatc aagattccct
     7561 tgtcatttta actcaaattc tctaaactat ttttaaaaaa aaacgacctt ttttttaaaa
     7621 aaagct
//
LOCUS       DAUCWU010000072         7429 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUCWU010000072 DAUCWU010000000
VERSION     DAUCWU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.072"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            1..358
                     /locus_tag="VGY24_001399"
                     /pseudo
     CDS             1..358
                     /locus_tag="VGY24_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            503..706
                     /locus_tag="VGY24_001400"
     CDS             503..706
                     /locus_tag="VGY24_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284375.1"
                     /translation="MNYLEYALVYLERELEIIDNEVIEVELPGGDWEFVPNPYYEKGL
                     HDSPHYRSQVAKDILDIKGLLGR"
     gene            794..1093
                     /locus_tag="VGY24_001401"
     CDS             794..1093
                     /locus_tag="VGY24_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284376.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEEYQFAEFVEKLEAL"
     gene            1093..2250
                     /locus_tag="VGY24_001402"
     CDS             1093..2250
                     /locus_tag="VGY24_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ4284377.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLA
                     MGTLEKLIGKTTFADLLAEVIDKPQGKPVLVKNSDKRPALNSLEQAIKDFE"
     gene            2259..2822
                     /locus_tag="VGY24_001403"
     CDS             2259..2822
                     /locus_tag="VGY24_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ4284378.1"
                     /translation="MIMTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKVTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHVFMS
                     VSSKTKPQIIDKYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            2865..4787
                     /locus_tag="VGY24_001404"
     CDS             2865..4787
                     /locus_tag="VGY24_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ4284379.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSHYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDTSGKNLIRYFSLPCKPSKANGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWDYYACDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSVNGLTKADVEQELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIVYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYGVVFHVHDEAIIEGSGLTIEEVNDLMAQAPEWAEG
                     LPLNSEGYVTKYYMKD"
     gene            4792..7176
                     /locus_tag="VGY24_001405"
     CDS             4792..7176
                     /locus_tag="VGY24_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ4284380.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWRDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDEVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDITPTNKLPSSK
                     AMNAFVSDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEAACETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYAIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKHQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMGEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLARNSFTQRMNELNFENKNAKMGGKTVRCWIGFRLKGEF"
BASE COUNT         2390 a         1333 c         1788 g         1918 t
ORIGIN      
        1 attaagaaaa ttaatcggat tgagggggag ttagttaaac ctgaagctga aagacgagat
       61 ggcttaccta tctgctcagt tgggttctat taaacaagat ttaaaattga tgctttggat
      121 tgagctaccg acgttaaatg gcgtagagaa atatgaagct ctttatcaag gaaatgattt
      181 taaattcagt gaaactttct tagagcgtaa agcgacaaga aaagcctttt ggagacgatt
      241 agctaaggaa acttttaaag aagaccagga taggaggaac tttgttaagt tggctgagaa
      301 agaatttaaa ggtgcaactt tgtcatggca agaatttttg tggggtaaaa agaaatgaat
      361 atatcactaa aatttattga tgaaacccta gctggcttaa atgatatttt acgtcagggg
      421 ggggctcttg ctcccaaaac caagccttag cagacgcagt ttttatacta acagctttaa
      481 aacaagtaat tgaggagaga aaatgaacta tttagaatac gcactagttt atcttgaacg
      541 tgagttagaa attattgata acgaagttat cgaagttgag ttgccgggcg gagattggga
      601 gtttgtgcct aatccctact acgagaaagg tctacatgat agcccacact atcgtagtca
      661 ggttgctaaa gacattcttg atattaaagg acttttgggg aggtgaggct attgattgca
      721 tcaaaaaaga cggcttagga aaccgtcaac aattatacac aattaaatta taacacagaa
      781 agagagaaac acaatgattg aagtaacttt taaagcagag agcatggcag gtgttttcga
      841 tgctatgcgt gaagctctgg atatgccgaa gacagtaacc aaggatgttg ttgaagaaaa
      901 accagctcct aaaaagaaag aagagtctgt aactttgaca ttagctgata ttaagaaaat
      961 ggctaaagcc aaggttgaag aagggaagtc aaaggacatc aaggaagtct tgaaagagat
     1021 gaacgtcgca aaagttggtg aacttgaaga ataccagttt gctgagtttg ttgagaagtt
     1081 ggaggccctc taatgccagt agaaaatcac gcactactgt ctgcttctag tgctcacaga
     1141 tggctttatt gccctatgct gccgagatta gaagcagact atcctagccg cgataccgta
     1201 tacactcaag agggcacaag cgcccatgag ctttctgaaa tcaaactgat gtacaagtct
     1261 ggtaaaatca ccaagcgtaa atttaacacg ttaaccaagg cttttaagga aaactcagac
     1321 ttctacaacg aagagatgga agagatgacg gaactttaca cagatattgt tatggagcac
     1381 ctaaatgctt atgagaacgc cgaaattgaa cttgaaaaac gggttgattt tagcgactgg
     1441 gtgcctggcg gttttgggac ttcggatgtt gtcattttag cggatggggt cattgaaatt
     1501 attgatctta agtatggcaa gggcatgcct gtgtctgcta accaaaaccc acagatgggg
     1561 ctgtacgctc taggagctta tgcttcctac gatatggttt atgactttga ccgtatcaaa
     1621 atgaccatta ttcagccacg tttagattcg gttagttctg ttgacattta cgtggaggag
     1681 ttactctact gggcggataa tgttgtcttg cctatggccg ctcaagcaga cgcaggcatt
     1741 ggtgactgga acctaagtga aaaagtattg cagtggtctc ctgtcgcagc taaattggta
     1801 ccaagagcgc aagaaaattg ggaattaatt gataaatatg actatcagga gcctgtctat
     1861 ttatctgatg aagccgtcgc agagattctt gacaaagcct cagctatcaa gaagtgggtt
     1921 gagtcagttg aagcctatgc cctgaaagaa gcactctcgg gaaaagaagt tccaggctat
     1981 aagattgttg aaggtagaag taatcgagtc atcactgata aagacaaagc aattgatatt
     2041 ttacaagata acggctttga cgatgagatt tttaaaccga aagagctgtt agcaatggga
     2101 actcttgaaa agttaatagg taaaaccact tttgctgatt tattagcaga agtaattgat
     2161 aaaccacagg gcaaacctgt ccttgttaaa aatagcgaca agcgacccgc attaaatagc
     2221 ttagaacaag caattaaaga ttttgaatag gagaaattat aattatgaca ccaaacacaa
     2281 ctaaagtagt aaccggaaaa gtacgcctaa gctatgtagc tttactagag cctaaagcct
     2341 ttgaaggtca agaggctaag tattcaacag ttatcttaat tccaaaaaca gacaaagtca
     2401 caatcaagaa aattaaagac gcgcagaaag ctgcttatga ggctgccaag gacaataaac
     2461 tcaaaggggt taaatgggag cgcgttaaga caacgcttcg tgacggcgac gaagaaatgg
     2521 ataccgaaga gcacccagag tacgctggac acgtgttcat gtcagtgtca agtaaaacta
     2581 agccacaaat cattgacaag tataaaaacc ccgttgactc agcggatgaa gtttactctg
     2641 gcgtctatgc tcgcgtatca cttaatgcct atgcttacaa cacagcggga aataaaggaa
     2701 tctcttgcgg attaaacaac gtccaaattg ttgctaaagg agactacctt ggcggccgtt
     2761 cgtcagctga tgcagatttt gacgagtgga acgaagaaga ggacgaagac gacattttat
     2821 agtagagggc ctctttagag gctcctcatt tttaaaggag aggtatgaga catttaaata
     2881 ttgatattga aacctatagt tcgaatgaca tcaaaaatgg ggtttacaag tacgctgacg
     2941 cagaagattt tgagatttta cttttcgctt actctataga tggcggagaa gtagagtgcc
     3001 ttgatttgac aaggcagtct ctacctgaag acatcaaaga tatgttattt gatgataaag
     3061 tccgaaagca cgcctttaat gcccaatttg aaagagtttg tctcagtcat tacctcggtc
     3121 taccttacta tctagatcct tgccaatggc aatgcaccat ggtgctggcc caagagttgg
     3181 ggctgccttc aagcttggaa aagtgtgcgc tgtatttaaa attagcgcag gaaaaagata
     3241 cctcgggtaa aaacttaatc agatactttt ccctgccttg caaaccaagt aaagctaacg
     3301 gtgggagaac tagaaactta ccagaacacg cccccgaaaa gtggcaaatg tttattgact
     3361 actgcatcca agacgttgtt gttgagatgg caattgccga aaaactagag tcagttcctg
     3421 tgcacgaccg tgaatgggac tactacgcat gcgaccagag aatcaacgac agaggcgtgg
     3481 cgcttgataa agagttagtt gcttcggcct tgtattgcaa agatgttaag atggaaagtt
     3541 tgtctggtga actaaaagct ctaacaggac ttgataatcc taatagcagg gcgcagttgt
     3601 taccgtggct aaaagaacac ggttattcgg ttaatggtct gactaaagcg gatgttgagc
     3661 aggagcttaa gacggccgaa ggagaactta agagagtttt agaacttaaa ctacaaaccg
     3721 ctatgtcaag tctaaaaaaa tatgaagcta tggaaagagc tatgtgctca gacggacgag
     3781 ttcatggact acttcagttt tacggagcta gcaggacagg aagatgggcg ggcagagttg
     3841 tccaagtaca gaacttagct aggaattata taaaggatct agatgatgct agagagtatg
     3901 ttaaaaagcg tgatattgat gctgtggaga ttttatacga tagtcttaac gacactttaa
     3961 agcagctagt aagaacggca ctcgtggcca aagactgctg taccttctac gtctctgact
     4021 tctcagcgat tgaggctagg gtgattgcgt ggtttgccgg agagcagtgg aggcttgacg
     4081 tgttttcgac gcacggaaaa atctatgagg catccgctag ccagatgttc ggaattccaa
     4141 ttgaggagat tgataaggaa ctacgccaaa aaggcaaaat ctcagagttg gcacttggct
     4201 atcaaggagg tcctggagcg ctcaagcaga tgggagctct aaatatggga gtcaaggaag
     4261 aggagctcca agggctagtt gatgactggc gcagggccaa taagaaaatc gtccaatttt
     4321 ggaaagatgt gcagagagcc gccatcaaag ctatcaaatc gagagcacca ataaaacttg
     4381 gaaaactacg atttagatac cgtaaaggtt tcctctttat aacattgcct agcggtagga
     4441 acttagctta tgcaagagcc aaggttgagc caggcgacta tggagacaaa atcgtctatg
     4501 agggccaagg agataaggcc tacttcacag cgcaagagac ttacggcggt aagcttgtcg
     4561 aaaatatcgt tcaggcgacg gccagggata ttctagctga agcgcttctg aggattgaag
     4621 ctgcaggcta tggtgttgtt ttccacgttc atgatgaggc tattatcgaa ggctcaggcc
     4681 tgacaatcga agaagttaat gatttgatgg ctcaggctcc tgaatgggcg gagggtcttc
     4741 ctttaaatag cgaaggctac gtaacaaagt attatatgaa ggattagata gatgaagcaa
     4801 gaaaaactaa tagtaaagtc ttctcctctg caagagcttc atatcgcaac gggtaattca
     4861 cgaacagcta agacgtggaa aaatattacg ctaacttggc aggagctggt tgagaggtta
     4921 gagaaaccta cagtcaccca agagacgttt gcggagtacc agaagatgtc tcgagcagaa
     4981 aaagggcaag caaaagacgt aggaggtttt gtcggcgggt ggctaaagca gggtaaacgg
     5041 aaaaacgaaa atgttcaaag taggtccttg gttgcgcttg acgcagatag cccaagtaaa
     5101 gatttcttag ataggctaga cctgcttgca gactatgcat acgtactcta cagtactcac
     5161 agccactcaa aaaaagctgc taagtaccgt cttattatcc ctactgaccg tttaatgatg
     5221 cctgatgaat atgagccagt cgctaggtac ttggctaatc aactaggcat gtcgaacttt
     5281 gatgacacga cttatcaaag tgtgcgtttg atgttctggc cgagccactc gagagatgct
     5341 gactttacgt ttaaatataa cgatgaggct tttctaagtg ttgatgaggt gcttgataca
     5401 tacccggact ggcgtgactc aagcttctgg ccagaaagcc cgacgcacgc tgttaaaaga
     5461 cagcgtgaag ctaaaaaaca aggtgaccca cttagtaaaa aagggcttat tggagccttt
     5521 tgtcgtaact atgacattag acaggccatt gcaacgttct tgcccgaggt ttatgaagaa
     5581 ggaacgactc ctgataggta cacctacact gagggttcaa ccgcaaatgg cttagtcatc
     5641 tatgatgaag tcttcgctta tagccatcac gggacagatc ccgtggggga tacgcttgta
     5701 aatgcatacg accttgttcg tatccataaa ttcggagagc aagatagcga ggctaaagat
     5761 attactccta ctaataagtt gccatcaagt aaagcgatga atgcttttgt ctctgactta
     5821 cccgaaatta aagactattt aatggcggag gctttaggcg attttgatga agagttacca
     5881 gtcgaagatg acagaagctg gcttgaaatt gatgagaggg gcgaacctga ggtcaatagt
     5941 tatttgctag caacgcagat tattaaggag gttccgattt attgggacgg cttagaattt
     6001 ttacgctacg acgctaaaaa aggcatctgg ttgccaaacg cagaggagta tttgaagagt
     6061 tatatctcaa ctaagaaact cggtaaaatt actaagatta ggcacattag cgaaaccgtc
     6121 gtagcgatta aggcacaggc tttctcaagc gaagtgttta ctgagagcga tcttaacaag
     6181 atagtgctag cgaacggagt ctatgacttg agggataaca gttttaagac taagtttgat
     6241 ccagaattgc atgcaaggtc aagccatccc gttgtctatg accccgaggc ggcctgtgaa
     6301 acctttgagg gttttcttag ggagaccgtc ggagctgaaa atatagattt catctttgag
     6361 tggttcggct ataactttta tcgcgaatat gctattcaaa aaatgctatt catctacggc
     6421 agcggcggta ctggtaaatc aacactgatt aatattttac gtgaaatgat aggtgctgat
     6481 aattactcag ccgtgacact gcagtacctg atgcaagaac gctttgcaaa aatcggctta
     6541 tatcgaaaga ctgctaactt cgataccgat gctaaacatc aatacttagc agatggcgca
     6601 acacttaaaa tgttgacggg ggaggatact attcacgcag accgtaaaaa taaagagccg
     6661 attaactttt acaattatgc taagctgtct tttgccatga atgagctccc acctatgcga
     6721 gatttcagtg ggggacttaa gcgccgcatg atgatccttg agatggataa ggtattaact
     6781 caggaagtta aggcaaaata ccccctagat aagattatgg gcgaggtgcc tggtatcttt
     6841 aacagagcga tggaggggct tagaaaggcc ttaagtaaaa gagatttcag tattagtgcc
     6901 agcatgaggt caagtgtcga gaaatgggaa aaaggcaacg atgtcgtggc tatgttcctt
     6961 gaagacgagt gtgaacttgg cgaagacttc aaagttcctg ttagggatgt ctacccagct
     7021 tataaattct attgtcagga ttcaggctac aaacctttgg ctaggaattc gtttacgcaa
     7081 aggatgaatg aactgaattt tgaaaataag aacgcaaaaa tgggaggcaa gaccgtaaga
     7141 tgttggattg gttttaggct aaaaggagaa ttttaaaggg ttacgttttt caacaaaaaa
     7201 ggtaaacagg agatataagg ttacgtagtt acgcagaatt cagactgata aaaaatttta
     7261 taagttgaaa aaaaacgtaa ctacataact ttcctgaaaa acctttcctg tttaagtcaa
     7321 tttacataac taacgcaact tttatgcaac ctcaaaaaac ctttcatagc aatagtttag
     7381 atcctaaggt tatgtagtta tgttttttct tctattaact taaatataa
//
LOCUS       DAUCWU010000073         7361 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUCWU010000073 DAUCWU010000000
VERSION     DAUCWU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.073"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            523..702
                     /locus_tag="VGY24_001406"
     CDS             523..702
                     /locus_tag="VGY24_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4284381.1"
                     /translation="MTKLERLQEENEYLRTEVAYLKKPRELRLKEASLQRRRQKTIIK
                     NGQRRALIRFSFKNG"
     gene            complement(851..1774)
                     /locus_tag="VGY24_001407"
     CDS             complement(851..1774)
                     /locus_tag="VGY24_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284382.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1767..2837)
                     /locus_tag="VGY24_001408"
     CDS             complement(1767..2837)
                     /locus_tag="VGY24_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284383.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2846..3772)
                     /locus_tag="VGY24_001409"
     CDS             complement(2846..3772)
                     /locus_tag="VGY24_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284384.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGVGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3772..5274)
                     /locus_tag="VGY24_001410"
     CDS             complement(3772..5274)
                     /locus_tag="VGY24_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284385.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMAGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5339..7309)
                     /locus_tag="VGY24_001411"
     CDS             complement(5339..7309)
                     /locus_tag="VGY24_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ4284386.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKADQNKDVVAAVGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2279 a         1436 c         1356 g         2290 t
ORIGIN      
        1 acaacttctt tattctatca ggtttctcat agcctgtcaa ctactttttc atcttttttc
       61 aataagtgtg ttacaggaac ctctctgaca gctttattag tatacgatat ctttactgtc
      121 attgtcaact ctttttttac tttttttgta cttttttatt tgcccgcgtt gtaaaatgaa
      181 gtgcaacaaa aaagagaaaa agccttgaac tacaccccaa acgttagaca aaaaaatcta
      241 acgttcgggg tattttttat gaaattaagt gatgaaggta cgaaatagac atcgttaaga
      301 aagaaaaaaa tacctactat ccaccagaga taaagcaaga aataatagat aacgttttga
      361 tagaaggccc gccacaatac caagtatcac ttgtttatgg cttgttacat gcaggaactc
      421 tcccaagttg aatagcacaa tataagaaaa acagttatac tgttgagaaa acaagagcaa
      481 gaccatcaca aacaggacgt aaaccaaaag aaacctgaag aaatgactaa attagagcgg
      541 cttcaagaag aaaatgaata tctcagaact gaggttgctt acttaaaaaa accgagggaa
      601 ctccgtttga aagaagcatc tctgcaaaga agaagacaaa aaaccattat aaagaatgga
      661 caaagacgag ctctgattag attttctttt aagaacggct aaattgactc gttctaccta
      721 ctattatcat cgattattgc aacaataaac gtatcaaaat aaaacgaaaa ggacttagtc
      781 ctctacaaca cagaactaaa tcctttactt caaatagagt agccaacttt tggggctcag
      841 tgcatctctt ttacaattct ttttgatatt tttcaatttc tgcttggttt gcccaaacaa
      901 agtgatttgg tttgatttca accattgatg gcttatctaa tgtataatca tgctggtctg
      961 gatgatagac aacaagttct ttttgacgct ctaaaattgg atctgggata ggcacggctg
     1021 ataacaaaga ttgggtgtag ggatgaattg ggttattaaa cagttcttct gtttctgcaa
     1081 cttctacaat aaccccttta tggataaccg caatacgatc tgaaataaag cgaacgactg
     1141 aaagatcatg ggcaatgaag agataagtca aacctttttc ggcttgcatt cttttaagaa
     1201 gattcaagac ctgtgcgcga acggaaacgt ccaaagctga aatcggttca tcagcaataa
     1261 cgaattcagg gttcattact agggctctag cgataccgat tcgctgacgt tgacctcctg
     1321 aaaattcatg tgggtagcgc gtcaaatgct ctgatagcaa accaacttcg gccatcatgt
     1381 tcttaatttt ttctttacgt tcttcctctg ttttaaacag attaaagtta taaagacctt
     1441 ctgaaatgat gtagtcaacg gttgctcgtt cattcaaact agcagcggga tcttggaaaa
     1501 tcatttgaat tttacgaatg agctcattgg cttctgattt tgattttctg ccattaatta
     1561 ctttcccatc gtataaaatt tgacctgaac tagtatcgtt caaaccaata atagcacgac
     1621 caattgttgt tttaccactc ccagattctc caactaaaga aaaggtttct ccttttttaa
     1681 taaagaaatt agcattttta actgcaacaa attttttctt tccttctccg aaggaaattt
     1741 ctaggtcttt tacttcgact aatttctcag acatttcctt cctcctgttg tgacattttc
     1801 cttaagattt tttgatgcaa atcttgaatg acttctggtt tttgaacttt tggagcctct
     1861 gggtgtaaaa gccatgtttt ggcccaatga gtctcagata cgttaatcgc cggaggtgct
     1921 ttttcaaagt ctaaaaccat agcatattct gagcgaagtg caaaggcatc tccgataatt
     1981 ggtgaataaa gtgatggagg cgttcctgga atagcgtaaa gttcaccaga ttcatctgcc
     2041 aactgcggta agctagacag caaactccat gtataggggt gtcttggatc atagaaaatc
     2101 tcttcgactg ttccaaattc aacaatttct cctgcataca tgacagccac tttatctgca
     2161 atgcttgcca caacacctaa atcgtgcgta ataaagataa tagtgaaatg atattctcgt
     2221 tgaagcgact tcaataactc aacgatttga gcttgaatag tcacatcaag ggctgttgtt
     2281 ggctcatcac agataagaat atctgggcga caagctaaag caatagcgat aacaatacgt
     2341 tgacgcattc ctcctgaata ctcaaatggg taatcttcaa agcgtttttt ggcatttggg
     2401 atacccactt tattcatgta atcaagggcc atttctttag ctttggcatg acttactttt
     2461 tggtgcttaa taatcacttc tgtgatttgg ctaccgatag ttttaatggg actaagactg
     2521 gtcattgggt cttggaaaat cgttgcgatt tttgagccgc gaatctttgc ccactcttta
     2581 tttgttttta aatctgtcaa ttcttgccca cgatagacaa ttgaaccatt agcaatgcgt
     2641 ccattagact ccaacatccc tgtaaaggtc tttgttaaaa cagatttacc tgagcctgat
     2701 tcccctacaa aagcaaggac ttctccttca acaagttcca gtgagacgtt acggatagct
     2761 gttaaaacac gatcacgcac atcaaattct accaccacat ctttggcagt taagattaca
     2821 ttattttctt ttgtcatttt aactcctatc tatgtgaacg tgggtcactg gcatcagcca
     2881 agttttgtcc gacaatgtat agtggtaacg atactaaaat caatgttact aagggaatcc
     2941 aaaagaggta ggcatttgtt gttaagttgc ttgaataatt agcaatcaaa cgtcctaaac
     3001 ttggagtagt ggtgggtaaa ccaaccccaa agaaggataa gaaggcctca gaagatacat
     3061 aaactggtag catttgtgac aacatagtca tgataactga aaccaattga ggcaggaggt
     3121 tcttaacagc aatcttgtac attggtgttc ccaaagtttg actagcaagg ttgtattcta
     3181 aatcacggta acgcaagatt tgaacacgga tggagtaggc gacaccaatc catccagtga
     3241 tacagaaagc tagaatcaaa ttccaaaatc ctgcacctaa tgaataggtc aaaacaatga
     3301 taataagcat agaagggata tttgagataa tgttataaat ttcaatcata actttatcaa
     3361 atgctttaga aactccccat atggctccta acactacccc aatggtgata ttaattagtg
     3421 tcgctataac tgagattaaa atagaattac gtgccccata ccaaacacca tcaaacagag
     3481 attgcccatt tttgtcggtt ccaaaccagt actctgcatt tggccaaata taacgctttg
     3541 aaaagtcatt gatattacta acgtcattaa agtcgtagtt ggcaaacatt ggataaataa
     3601 agctcatcat caagactgtc actaaaatca cgagcataaa gactgtagat tttttagaaa
     3661 aaaactgacg aaacactgat ttccagtaag aataagcagg ggtatcaatc acttcggagg
     3721 cttcactatc gcgctcaaca aatcgaaatt tagatttatc aatcgattcc attacttacc
     3781 tccttttttt gttgacaatt taatacgtgg atcgacaagg gtcatgacaa tatctcctag
     3841 taaaagtgaa acaattgaca agacagtgaa gataaaggtt aatccaacaa tcatagaatt
     3901 gttagcagat ttaatagaat caattaacat tttacccata cccgggaagg caaagacagt
     3961 ctctgttaag gttgcaccac caatagcaag gataatactt gcaggaaccc ctgacacaat
     4021 tgggaccatg gcatttttga agagatgccc tctgtaaatt tcagattctg ataatccttt
     4081 tgaacgcgca aaacgtaccc aatcactggc ctgaaggtca acaagatagc gacggaacca
     4141 gataaccgta gttggaatat ttaaaattcc taaaataagg gctggtaaaa tataagattt
     4201 aggatcagat gcccctagca ttgggaaact atctggtaaa ccagccatgc cacctaggaa
     4261 gcgaacaaca tagataacag caatacttgg taaagcaagc atgaaggtca tggtcgctgt
     4321 tgagaaacgg tcaaaatagg tatttttaaa ccgagccata aataaaccaa gaggcaaacc
     4381 aacgatatag gaaaacataa caccaaaaaa accaattaca aaagaattgt gaatcattga
     4441 tgggtcgacg taattattga tggttttagt gtaggaatct cccttaccat agtttacctt
     4501 ggtgatatca tccaatgatt ttggattttt gtagctacgg ctatagatat ccactgacga
     4561 tttcttagtg acacctgatg ggaaggtcac atcttgaaga gctgttcgtc cttgcccttg
     4621 tgaaataacc tgaagaacag gaatattgct gtatgttggg taagatgttc ctaagtttag
     4681 ggtaacaaag ttttggtgaa gataaggaaa ttttccgtta gtgtagagga gatatttatg
     4741 ttttgtcccc gaaccaacaa gtgaccagcc aactgattta tctttttcta gacgaatata
     4801 cctagctaat tttggattgt ctttatcctg aatcttccaa gggtgatcaa taacaactag
     4861 atttgagaaa aagttccaaa cacgttcgta aataggaata ttgcgaattg cataaaactg
     4921 tttgctagtg gggaagcgtt ttagctgcca gccattgcca agagaatcaa tatacttctc
     4981 atagatagct ttgttagcac tagttgcttc tgtcgttaca ctactatcta ctttagaagc
     5041 attatctttt aattctttgc ttgaaaaata attgacataa cccatacgtt gaaaagttag
     5101 attttcatac gctacttttt tatctggtgt tgttgtcatt ttattataat tagggtcttg
     5161 tttaaagata aggctggtag gtactagagt atatacgatg gtgtatgtta aagccgttac
     5221 caaaactacc gacactaacg atcgcatgat acggtttaaa atgtattttt tcatagttat
     5281 cctcaatagt tcaaaagaac ttcctcacat aaacgagaaa gttcttttga acatcgtttt
     5341 atttttcaac gtgatcagct aacttctcag catattttgc atttgactta gcctttgctt
     5401 tcatccattt ttcttttgca ttttcgtatt gttttgctgt gacaggtttg tcttgaagtt
     5461 tcattccttt atatgctaga ggacctttag accctgccca agagaagccc ccgctaaatg
     5521 gaacggcttt agtaactcgt ggagtgccac ccaatgccac aactggaata tctacggcac
     5581 tatctgtaag gtaggcttgt gcttttgcgt aagctttata gcgcgcatcg ttgtcgtctg
     5641 taattgctgc tgcttcatca agaagagttt ggtaggtatc aaggcctaca gctgccacaa
     5701 catccttatt ttgatctgct ttgattccaa gtttttggat aacagatcca ccacctactg
     5761 gactcatgat gtcaaggtag gtccgtggat cttggtagtc tggtccccac catgatgaaa
     5821 taatatcgta gtcttgttgt tctggggtct cagcatagaa gccttgggct tcgtgagttg
     5881 atgtttctgt ttcaagaaca ttgacaatga cattttcttt accaagagat gcttcaacag
     5941 attgtttgaa agactgggct tcctgaacag ttgctgcgtt tgcctggtca acagggtaat
     6001 ctaattgaac tgggaaggtt acaccttcag ctgtcaaagc ttctttggct tttgcaaatt
     6061 caacttttgc tttttcagga ttatagaaac catcttgagc atcagctaag ttaacgtctt
     6121 tccattcatc accaagtttt gccatttcct tttcaacttc tgagccaaaa tcactttctc
     6181 caacggttac aaaagttggt ggcacaagca tgttacgtaa ggcttttgtt ttggcatctt
     6241 gacctgcagt ttgtgcttgg aatgacgctc ggtcaaaagc aaactgaata gcttgacgaa
     6301 aatccttgtt gttaagagct ttcttaccgg catcttgttg tgcagggtct ttcttagtgt
     6361 ttttgaaaga agtgcggttc aaattccatg ttaaatgacg gatatctcca gtcaacattc
     6421 cgtaagtaat attatcagca tagtttttct tagctgattt gtaggtaggg tcatttgggt
     6481 aaagtcgtgc aacgctgaac tcacccttgt caaagttctt gtagaacgaa cctgggtctg
     6541 aaccatctga gtaagtcaat ttaacagatt ctatcccaac attcttagca tcccagtagt
     6601 tttcattttt atggaattcc atagatgatt ttgaggtgaa ggcgctcaag aagtaagcac
     6661 cattaacaag gattgatgat ggatcggttg taccaaaatc tttacctttt gacttcaaaa
     6721 atttcgcatt aactgggaaa agcacactat aagttgtttt tgaattccag tagctttcag
     6781 gcttgttcaa agtatactga acagttttat cgtcaagggc tttgacacca acttctttaa
     6841 aatctacttc accattttgg taagccttta agttttttat tgaatcttca acaacgtaaa
     6901 gagcatctga tttatcgtca accgcgtgct tcaaaccagt cacaaaatct tctgctgtta
     6961 ctggggcata ttcttcacca tcagccgtat accaagagac accatcgcga agagtataag
     7021 tataagtcag accgtctttt gaaaccttcc aatctttagc aagtgatggt actaaattac
     7081 catactcatc gttttccaaa agaccatcaa ccatttgtgt tatcacatca gtcgttccac
     7141 cgccattagt caaaatataa tccaatgatt ttggatcgtt aacaaaaacg tacttgtagg
     7201 tttttgtagc ttctgagcca cctgaagcat ttttattacc acaagctgcc aaagcggata
     7261 ctgacaagat cgcaacactt acagctgcca accatttact tttcttcata aaagtcaccc
     7321 ctaaacttta tttttcattt tttggcaatt gctaaatgaa a
//
LOCUS       DAUCWU010000074         6913 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUCWU010000074 DAUCWU010000000
VERSION     DAUCWU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.074"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1136)
                     /locus_tag="VGY24_001412"
     CDS             complement(<1..1136)
                     /locus_tag="VGY24_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ4284387.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMVENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAILKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVA"
     gene            1467..2429
                     /locus_tag="VGY24_001413"
     CDS             1467..2429
                     /locus_tag="VGY24_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ4284388.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFNSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(2546..3781)
                     /locus_tag="VGY24_001414"
     CDS             complement(2546..3781)
                     /locus_tag="VGY24_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ4284389.1"
                     /translation="MIKRWAILFFVVLTCSGLSKIVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDPVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3939..5126)
                     /locus_tag="VGY24_001415"
                     /pseudo
     CDS             complement(3939..5126)
                     /locus_tag="VGY24_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(5129..5316)
                     /locus_tag="VGY24_001416"
                     /pseudo
     CDS             complement(5129..5316)
                     /locus_tag="VGY24_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            5403..5546
                     /locus_tag="VGY24_001417"
     CDS             5403..5546
                     /locus_tag="VGY24_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284390.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            complement(5617..5835)
                     /locus_tag="VGY24_001418"
     CDS             complement(5617..5835)
                     /locus_tag="VGY24_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284391.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFYKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(6027..6749)
                     /locus_tag="VGY24_001419"
     CDS             complement(6027..6749)
                     /locus_tag="VGY24_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HEQ4284392.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLW
                     LCYCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTG
                     VALGISMVGVIAAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVS
                     CTVALLHIRIFKRWNQDCLTSS"
BASE COUNT         2289 a         1384 c         1045 g         2195 t
ORIGIN      
        1 gcaaccctac caaaacgttc tgggtatttt tccaatggat aggcgttagg gtaatgaaag
       61 ctagtaacct gcaagtcacc tatttgctca agagcaacca acatatctgc aataggcttg
      121 gtgtcgatcg caccaaacaa aatatgaagc tttttatcat tgtagttatt ttttaagatg
      181 gctaccaaag cagcaacgct ttcattgtta tgagccccat caatcattag atttggtgct
      241 aataattcgg tacggcctag ccacaaacta tttgctagac cttcccttat atgatctgaa
      301 gttagcctag gatagcggtc tttcaaggtt aagcaagtca taatagctaa agctgcatta
      361 gacacttggt ggtgtcctgg catagcgatg tgaatatctg aaataactcc taaaggacta
      421 gtaaaacgat agccagatgc gttctcaacc atttgaaact gctcttgcca ttcccagatt
      481 gaagcaccaa cctgctcagc ttttgttaaa aaaacctctc gagctgacgg gttttcaacg
      541 gcaaaaacaa gggtctcgcc cccttctaaa acaccagctt tttgggcggc aatatcagcg
      601 taagtctctc ccaaaatagc ttggtgatct aaaccaattg agggacagac cacaaccatt
      661 gcttgaaaaa cattggtcga atcataaaga ccgccaaggc ctgcttcaat aatagcaata
      721 tctacaggat gcatatcacc aaaatagagg aacatgatca aagtaatcac ttcaaattca
      781 gttacctctc caaaatcagt ctcttgaact agacgctctg tcaaaggacg aatacgatta
      841 gcagcaataa ccaaatcttt ttctgaaatc attcgaccat ttatgctaat acgttctttg
      901 aaatccataa tataaggaga ggtaaaggtc cccacttcat aaccagctgt cgtaaagatg
      961 tgttgtaaat gattaaccgt ggaacctttt ccattagttc caacgatatg cactcccttg
     1021 acattttttt ggggatttcc taactgtcca agaacccaaa gcatacgttt taagcctggc
     1081 ttgatgccga aaactaagtg atcatgaatc cattctagag tctcttcata agtcatttca
     1141 tacctcacat ctatctttag tataccatat ataaaaaaaa gtgacagcag ataacctaga
     1201 gtagtaccct tttttttatt atcctcacca aacactcaaa taagttctaa agtacactac
     1261 acttttatct tcattgcttt ttttacgtga taagaatacc agcctcacat agtctttgct
     1321 acctcctaaa gtgttatctg tgacaatatt tttttactcc ttaatacacg ttcttccaaa
     1381 aaacaagaaa aaaggctgac ttcaagcctt cttttctcta aaaaatgtta caatagaggt
     1441 acttatatca aaaaaaggag caagtaatga acaatagaca taaacggcgc caaaaaagaa
     1501 aagccttcac tctcattaac cttatactat tatttgcctg cattcttggt ttgaccctca
     1561 ttttctattt atatcaaggg tctaacaaat tcggcttgtc agatgtctct caatttgtac
     1621 ctaaaataag tctttttcat cacaaaaaga cggcaaaaaa agaaacgaca aaacttaaga
     1681 agactcattt caattcctca aaatctcaaa aaaaggccca ttctaaatta acttggacca
     1741 agcaagaaac acctgtcaag atcccaattt taatgtatca tgctattcat gtaatgtccc
     1801 ctgaagagac ggccaatgct aatttgattg tcaacccaga tctctttgat caacaacttc
     1861 aaaaaatgaa agacgagggc tactattttt tgagtcctga agaggtctac cgcgctcttt
     1921 caaataatga attgcctgct aaaaaagttg tatggctaac atttgatgac agtatgattg
     1981 atttctacaa cgttgcttac ccaatcttaa aaaaatatga tgctaaagcc accaataatg
     2041 ttattacagg actaaccgaa atgggaagcg ctgccaatct gactcttaag cagatgaaag
     2101 aaatgaaaca attcggtatg tctttccaag atcatacagt gaatcatcct gatctagagc
     2161 aggctagtcc tgatgttcaa acaactgaaa tgaaagattc aaaggactat cttgataagc
     2221 aattaaacca aaacactatt gccattgctt atccatctgg tagatataat gacactacct
     2281 tacaaattgc agctagactt aactataaat taggtgttac cactaacgaa ggaatagcta
     2341 gcgcggctaa tgggcttctt tctcttaaca ggattcgtat cctcccaaac atgagtccgg
     2401 aaaatctttt acaaacaatg gaaccataac atcaattttt ttatcattcc aaatcatact
     2461 cactgtaaac taaaaagaca gttatgaaaa aattctttca taactgtctt tttttgattg
     2521 ttttaaaatt attttattct tatctttaac gtcttttttc aagaaaatct cgccaccaca
     2581 gctgtaagtc agaggctttt ttaagagatt ggctagcttt aagaggaata cgcttaggtt
     2641 cctttaaata cctcactgac tgatctgtta ttcttggaga cgtcaaataa gcaaccactt
     2701 cacctttagt aatcggtgct agtaaagaag tctctgatat cataaattgt ttaggtttgg
     2761 gaacttgttc accctgttgc aaaatgatgg ttacatcttc ggcaacagat accggtagtt
     2821 cttttacttg gccatcttga atagggagcg accgctccga gacaagactt ccttttttaa
     2881 gaatcttacg acgacaataa gttctagcca catagtccaa caaacgattt gcttgctcaa
     2941 accgcgtgtt ttggttttta tcagatccat cggcatttag aataacggta atcactcgca
     3001 taccattttc aatagaagtt accacaagac aatgacctgc caagtctgtt gtcccagtct
     3061 ttaaaccatc cactcctgct ctacccttag ccatacctgg taagagttga ttaaaacttt
     3121 taatagaatt gccagcaaaa ttaacttctg tttgtttggt gatctctaaa atctcaggaa
     3181 aatcttctaa caaatgcatc gtcacaactg caacatcttg agcactcaag gtattttctt
     3241 cttccaacgc agaaccagga taacgattat cctttaacac ctcatttggc agacctgagg
     3301 cgttgaggat tttaccagaa gtaatccccc aatgagatag ctgttctctc atttgattta
     3361 caaaatgagg ctctgagccc gccacttttt cggccaacgc aatagcagcg ctatttgagc
     3421 ttgctaccaa agtagctgtc aacaggtctt gaactgaata agtttttttg tcaaaaggta
     3481 cattacttaa agacctatct tttgttaact caaaggcata atctgagaga ttaactggat
     3541 ctgataattg cagctggcct gatttaacct tatctaaaac cagataagcc gtcaccaatt
     3601 tggtcaaaga ggcaatagca tctggcgtct tagcgtcttt ctcatacaga actctcccag
     3661 agtctgcctc tacgacaatc gcatgttctg ctcccacttg aaaatcagct gcgaggacta
     3721 ttttactaag ccctgaacaa gttaatacca caaaaaaaag gatagcccac ctttttatca
     3781 ttatatcttt catagttact attatagcaa aaattctcag aaatttggaa ggaagtatag
     3841 tggcacacac ctttagtagc ttaaacaagc ccataaagca agtaccctgt aacaataaca
     3901 catcctgtta tatgggcgat ataataagct tcgttatatc aattagttgt ttggcaggaa
     3961 ttttggtata aaatggaatc taatcctgaa cttattacat tttgggttat ttcttcagat
     4021 actgctttat ctaatgcatt aaaccagtga ctatatgtat tcaaagttgt agacttatca
     4081 gcatgtccca tacactttgc aacgtacaga atgtcttttt tcaaaacatt aataagatag
     4141 gaagcataac tatgtcgcaa tccttttcca gttattacgg gaactccaat actttgagct
     4201 ttgtgcttaa taactcgaca aatcgttgat ttacaaaatg gttcaccaaa ccgtgaaata
     4261 atgaaatctg tgtcagtatt gactacttga tttcttctcc aattttccaa tactactatt
     4321 gtcacatcat ctaactcaat aagacgttct cctgctggcg tttttgtttg gtctttacga
     4381 taccaatttc cattttcatc cttttccaag gttgtatgaa ctttcaagaa tttttgttcg
     4441 aaatcgatat tttcccatca taatgataaa ccttcactta cacgaacacc tgtcatataa
     4501 tatagccaaa tagttgtaaa tctttgtagc tcttcataat cttgcaaatc aaacgatttt
     4561 ataaaattct gaaactcaat ataagtccaa aatggagtgt cgggatgttt ccctttagga
     4621 ttatccaaag ccttacatgg cacttttgaa atatagtcta acctttcagc ataccccatg
     4681 caagctttga atcgcgacca taaattcttt gcataatttt caataatatg taaactaaaa
     4741 gattcacaat ctcaagctgt tattcccttt aaaagtttag aatcaaaata ctgaataaat
     4801 aacttatgat gtggtaaggc tatttcataa gttacagcct gaaccttttg cttatatgct
     4861 cttaggtaaa tagtattcat atagttttca aacgatatat tgtcattttc aatactaaga
     4921 ttattactaa acccatacct aatccgaacc agttcatcat acgcttcttt cattgtacca
     4981 aatggctttc catactaatt ctttctggct ttcttttgaa ctcgtttacc tgttactgga
     5041 tctacaccaa gatcagcttg ataaaatatc ttcccattct tatcacaata aacacctttg
     5101 tacttttttt gattttttat agtcatcttt aattctcact ttctaaactt tcttcatctt
     5161 taggagcaat accaagtctt ctattgtcaa agggcgattt acttaattgt accgcatttt
     5221 gttcaagttc gcgagcttct tcaacttttt tatagctatt ctttttgctt ctctaataat
     5281 atttcttgca gtatgttcga gaaaaccaat attcatcaag ctttttatat gttactattt
     5341 tcacacctcc actcctttca aaaaaattac aacctccgtg ggtatgactg gttaaatcac
     5401 tcatgggaat ctcacccact ggccttttac gctggcagcc tgaacgttca gaagtatcat
     5461 tgtccctatt tgtggtcatg gcagatagag aaccactcta ttttatagct aataattatc
     5521 gctcctatca cggcgtatct cactaaattg ctcgacaaac aagaaagtcc cacctcggtt
     5581 tattcagttg tcaatgaaca aatcaaggcg agacttttat ttattatgaa aacgatacgg
     5641 ataatctgtt tgccctaata aataatcaat actgacgtta tagagtttat aaaactgcac
     5701 aagaacatct gccattaagg caacctctcc tctctcaatt tttgcataat ttgcatgaga
     5761 gaaggaaagt aaatttgcaa caaacttttg agtgaaatcg ttatcttctc tcaaattcct
     5821 aatacgcgga tacatctaac attctcctaa tatcagtata aaggagattt gattaaaaaa
     5881 tgtttaattg tcctatttgg acaagactat taaatataag ctagtaatcc attgaaattt
     5941 tatagtcatt ttttagttaa acaagttaga tagtatttat aacttaaaaa atcatattta
     6001 ttgctaggta aacaaaatag atgtatttaa ctacttgtca aacagtcttg attccacctt
     6061 ttaaatattc gtatatgaag taaagctact gtgcatgaaa caaaaaatat aacaataagt
     6121 ggaacaattg ccattttctg ttggttatat agagtatctg atattcttaa aagccatgcc
     6181 catggaataa gaaaccatac cttatcacca aggctcgttt cacaaaatgc tgcagcaata
     6241 acacctacca ttgaaatacc taatgccaca cctgtactaa atatatagct aataatatga
     6301 ttgatcagat aggttacata ggcaaaaatg atataaatta aactattcaa aataagtagt
     6361 ctgagagata catttatacc aatagttttc aataaaagaa taaatatagt aattgaaata
     6421 attaatacta tacaatagca taaccataga aatactaatt ctacaataaa tggtttataa
     6481 ttcgactgtg agtaacccaa taaatattta tattttccag atgattcttc taaatttttt
     6541 agttgaatgc acacaatact tgcaacaaaa ggaagaagta cacctactat ttgaaagtag
     6601 gagaataata cagtcctgct ttgtaaacct ccacgttgac tccaatagaa aaatagaagt
     6661 aagggaaata atagtgcagc taaaatatgt attagtaaat atgaagtatt tttgattttt
     6721 aaaaaattag aacataaata tgatttcatg actatttcac ctgttttttg aaagagatag
     6781 caaatagatt ggtcagtaag atgaaaaaag caattcctag gaacaatgat ataaaaatct
     6841 gtgagtagtc tggaacaaac tccgctccaa aaggtatact atttattctc attcttgtca
     6901 ttggctcaag caa
//
LOCUS       DAUCWU010000075         6877 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUCWU010000075 DAUCWU010000000
VERSION     DAUCWU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.075"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            5..78
                     /locus_tag="VGY24_001420"
     tRNA            5..78
                     /locus_tag="VGY24_001420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            158..231
                     /locus_tag="VGY24_001421"
     tRNA            158..231
                     /locus_tag="VGY24_001421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            227..808
                     /locus_tag="VGY24_001422"
     CDS             227..808
                     /locus_tag="VGY24_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ4284393.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEETGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGECFKGRRALLRKISPYFKEFAQQL"
     gene            993..1742
                     /gene="truA"
                     /locus_tag="VGY24_001423"
     CDS             993..1742
                     /gene="truA"
                     /locus_tag="VGY24_001423"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ4284394.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1732..2496
                     /locus_tag="VGY24_001424"
     CDS             1732..2496
                     /locus_tag="VGY24_001424"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ4284395.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            2486..2956
                     /locus_tag="VGY24_001425"
     CDS             2486..2956
                     /locus_tag="VGY24_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4284396.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2970..3530
                     /locus_tag="VGY24_001426"
     CDS             2970..3530
                     /locus_tag="VGY24_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ4284397.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVSQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(3595..4440)
                     /locus_tag="VGY24_001427"
     CDS             complement(3595..4440)
                     /locus_tag="VGY24_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ4284398.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            4761..6044
                     /locus_tag="VGY24_001428"
     CDS             4761..6044
                     /locus_tag="VGY24_001428"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ4284399.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            6261..6836
                     /locus_tag="VGY24_001429"
     CDS             6261..6836
                     /locus_tag="VGY24_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ4284400.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
BASE COUNT         2289 a         1001 c         1489 g         2098 t
ORIGIN      
        1 tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gagagaggta ggaagcgtcc tatctttttt tgcattttag
      121 agaagagtgt ggtaagatag aggcaagata tgcgttggca cccttagctc aactggatag
      181 agtacctgac tacgaatcag gcggttagag gttcgactcc tctagggtgc ataatgaaaa
      241 aagcaacagg acattagtgt cctgttgttt ttttgtggtt ctctttatac tagggatagt
      301 taaaaagaaa aggagactca aaatgtcgat agagacaaga gcagcgtttg aaaaagttaa
      361 gcccattatt ttgaaattaa agcgacacta ttatattcaa ttgtgggata gagatgactg
      421 gttacaagaa ggacatatta tcttattaca gttactagaa aggtacccag agttaattga
      481 ggaagaagag cgtttatatc gctattttaa aacaaaattt tcatcttatt tgaaagattt
      541 attacgccgt caagaaagtc aaaagcgtca gttccataag ttagcatatg aagagacagg
      601 ggaggttgca catgccattc catcgagagg gttatggcta gacgactatg tggcttatca
      661 agaggtaata gctagcttag agaaccaatt aaattcgcaa gagcgtatgc agtttcaagc
      721 acttatcagg ggcgaatgtt tcaagggaag acgtgctcta cttaggaaga tcagtcccta
      781 ttttaaggaa tttgcacagc agttgtaagc aagacctata aggaattggg ggtaaatcta
      841 tgaaaaaagg agagagtttg gaggaaatag ctttcttata gcttttaaat cgctagtagc
      901 ttagagtaag cttcaatacg tctttcattt gtttgggtac agtttgatta aaaatagttt
      961 ctcttacgtt taagaaatgt taaaatagta acatggtaag atacaaagca acgatttcct
     1021 atgatgggac tcttttttcg ggttttcaaa gacaaagaca tttacgtacg gttcaagaag
     1081 agattgaaaa aacactctat aaattaaata acggtacaaa aattataatt catggtgcag
     1141 gacgtacgga tgcaggagta catgcttatg gacaggtgat ccattttgat ttgcctcaag
     1201 aacaagaagt tgaaaaatta cgatttgctc ttgatacaca gactcctgaa gatattgatg
     1261 ttgttaatat agaaaaagtg gctgatgatt ttcattgtcg ttatcagaag catttaaaaa
     1321 cttatgaatt tttagttgat aatggtcgac ctaaaaatcc gatgatgcga cactatacca
     1381 ctcattaccc ttatactctt aatattaaac ttatgcaaga agctattaac ggtctggtag
     1441 gtacacacga ttttactggt tttacagcag caggaacttc ggtacaaaat aaggtgcgaa
     1501 cgattaccaa agcaacggta tctcgggatg aaaaaactga ttttttggtg tttacttttt
     1561 caggaaatgg ttttctctat aagcaggtga ggaatatggt gggaactttg ctgaaaattg
     1621 gtaatggtca aatgccagtt gaacaagtta aagtgatttt gagctccaaa aatcgtcagc
     1681 tagctggacc aacgatatct ggaaatgggc tttacttgaa ggagatttgt tatgaaaact
     1741 gattatatcg tgactatatc tgggaacgat attttaagtg gaggtggtct ctatgctgat
     1801 ttagcgactt acataaggta tgacctacag gcctttgtag cggtaacttg tttaactact
     1861 agatctgaag agggctttag tctatttccg gtagctaaag aaattttccg tgatcagttg
     1921 aacagcttta cgaacgctcc aatttctgct attaaaattg gtttgttacc taatgcggaa
     1981 atgtgtgaaa tagttttgga ttttataaag gggcatctgg gaatcccagt ggttttagat
     2041 cctgttctag catgcaaaga aatagatgat gttaaaatag ttcctctaag acaggagatt
     2101 ttacaattat taccttatgt gactgttgtt actccaaatt tggtagaggc tcagctatta
     2161 tctcaaaaag agattgtttc tttaaaagat atgcaagaag ctgctaaata tttttatcaa
     2221 cttggggcaa aacaggtggt tatcaagggt ggtaatcgat ttagtcagaa aaaggctatt
     2281 gatctttttt atgacggtaa agagattgtt accttggaat gtcctgtact tgagaaaaat
     2341 aatattggcg caggctgtac ttttgcatct agtatcgcta gtcagttagt aaagaaaaaa
     2401 acgccactag aagctgtgaa aaactcaaaa gagttagtct accaagctat tttacaatct
     2461 gatagatacg gagtaaaaca aagttatgca aaatagtaag atacgacaaa tgagtttaac
     2521 agggatattg acagcattgg ttgttgtttt aggaagattt gtcatgctac ctactccaac
     2581 aggtttttta acgctgttgg atgcaggaat ttatgctgtc agcttttcct ttggatcagc
     2641 tcaaggagct attgtgggag ggttatcagg ttttttaatc gatttagtgg caggataccc
     2701 tcagtggatg tttcatagtt tgattgctca cagtgtccaa gggtattttg cgggatggag
     2761 agggcgcaag cgttggcttg gtgttgtgat aggttctttt atcatgattt tttggtactt
     2821 tttaggctct cttatgcttg gttatggcct ttcgggatct ttagccggta tttggggaaa
     2881 cgtcatgcaa aatacattag ggctttttgt tggttttatt atctttaagg ccatattaag
     2941 gcaaaaaaag agataaggag agtgagaagt tgttgaataa tttagagaaa caaacaaggg
     3001 aaatcgtcat tgatgttgta gaacgctctg ctattcaacc aggtaatctt tttgttctag
     3061 gtctttcatc tagcgaaatt ttgggtagta gaattggaaa acaatctagt ctagaagtaa
     3121 gtcaaatagt tgttgaagtt gtgttagatg aattaaataa gcgtggtgtt catttggctg
     3181 tacaaggttg tgaacacgtc aatcgtgcct tggtagtaga gaggcatgtg gcagaatcta
     3241 agcagttaga gattgtcaat gtggttccga atcttcatgc tggtggcagt gctcagatgg
     3301 ctgcttttca atttatgtca gaccctgtcg aagtagaaga agttattgca cacgctggac
     3361 ttgatatagg tgatactgct ataggaatgc atattaagcg tgttcagatc cctctgattc
     3421 cttgtcaacg agagctagga ggtgctcatg tgacagcttt ggctagtcga ccaaaattaa
     3481 ttggtggagc tagggctgat tacaatatgg atattatccg taaatcctaa accctttttt
     3541 ctggaataaa acaattggtg ttttttctag aaaaaaggat tttttaagat tatattaaga
     3601 ttttgaggtg atagaatagc tgattgcagt cggtaattga atgccttctt tgagtaaagc
     3661 ttcttgataa agtctataaa aagtatggta aatcttaaat tgttctccat tttctgtgaa
     3721 aatagcaatt ctaaaagtga actgtccatt agagttattt tgaggcccta aaatatttgg
     3781 tttgccaacg atttgaggat ggtttggcaa ttccttttga ttcacttcat caatgattcg
     3841 agtgacttgg ctcaaattaa cagtggaata aagaggaatt tcaattaagg cacgcatatt
     3901 accacgagat ttgttgctga caacggtgat gctacggttt gggatgaaat gtagtgttcc
     3961 atcgaatcca cgaatttgag tagttctaat tccaacacca aagacactgc cttctatgtc
     4021 agagatagta acgttatctc ctacttcaaa ttgattctca aataagataa aaaagccatt
     4081 gacaacgtca gataaaaatc cttgtgcacc taacccgatt gctactccag caattccagc
     4141 tcctgctaga agactagaga caggtatgcc aaataagcta agtatccaat aaatgagaag
     4201 aaaatacaat aagtaattta aaatgttgtg agtgagctta gagagtgttt ttttgcgagc
     4261 ttcactttgt cttgaataag caaacgattt gttaatggtt ttttcaaaaa gatagttagt
     4321 aacacgtttt aaaatagcaa aaaagagtaa tagaataatg agagacacta gctttttaaa
     4381 aatcgttaag ccaatatttt cgatatgaga ctgctctagg taatgtaata taaatgtcat
     4441 gagaaatcct ttctttgata gtttatcaaa aaaaaaccaa tcttgaaaag agataaatta
     4501 atattttaac caaagttatg ataattgttt tatcataaag aatgccatag tcatccgttg
     4561 tgataatgct tgaaaatagt cagttagtaa gcgaaaaagt ctaacattaa cctttataaa
     4621 ttgaaaataa ctgttagtag actaatgaca ggtagatact cttaatagcg cgtttgattt
     4681 cacgcataaa aagctttaaa aacgctttaa attatgataa aatgaactgt atcgaaatta
     4741 ttttaaggag aaatgactta atgtctacat catttgaaaa caaagctaca aatcgtggcg
     4801 tgattacatt tacaatcagt caagataaaa taaaaccagc tcttgataaa gcttttaata
     4861 aaatcaaaaa agacttaaat gcaccaggtt tccgtaaagg acacatgcct cgtccagtct
     4921 tcaaccaaaa atttggtgaa gaagttcttt atgaagatgc tttgaatatt gtattgccag
     4981 aagcttatga agcagctgtg acggaacttg gtcttgatgt ggttgcacaa ccaaaaatcg
     5041 acgttgtgtc aatggaaaaa gggaaagagt ggacactttc tgctgaagtt gtgacaaaac
     5101 ctgaagtgaa acttggtgat tacaaaaacc ttgttgtaga agttgatgct tcaaaagaag
     5161 tttcagatga agacgtggat gctaaaattg agcgcgaacg tcaaaacctt gcggaactca
     5221 ttattaaaga cggtgaagca gctcaaggtg acactgttgt gattgacttt gttggttcag
     5281 ttgatggtgt tgagtttgat ggcggtaaag gagataactt ctctcttgaa cttggttcag
     5341 gacaatttat cccaggtttt gaagatcaac tagttggtgc taaagctggc gatgaagtag
     5401 aagttaatgt cacattccca gaatcttacc aagcagaaga tcttgcaggt aaagccgcta
     5461 aatttatgac aactattcac gaagtcaaaa caaaagaagt acccgagctt gatgatgagc
     5521 ttgcaaaaga tattgatgaa gatgttgaca cacttgaaga cttaaaagta aaatatcgta
     5581 aagaacttga agcagctcaa gaaactgctt atgatgatgc tgttgaagga gctgcgattg
     5641 aattagcagt tgcaaatgct gaaattgttg atttacccga agaaatgatt catgaagaag
     5701 tcaaccgttc agtgaatgaa tttatgggca acatgcaacg tcaaggaatc tcacctgaaa
     5761 tgtacttcca attgactggt acaactcaag aagatttaca taaccaatat tcagctgaag
     5821 ctgacaaacg tgttaaaaca aaccttgtta ttgaagcaat tgctaaagca gaaggttttg
     5881 aagcaacaga tagtgaaatt gaacaagaaa ttaatgacct tgcaacagaa tataacatgc
     5941 cagctgacca agttcgttct cttctttcag cagatatgtt gaaacatgat attgcaatga
     6001 aaaaagcagt tgaagtgatt acaagcacag caagcgttaa gtaatcttat taaggaacaa
     6061 aacgtacaag ccggattaga acgtctttct agtctggttt ttctttttct aaagaaataa
     6121 taaggaatta gttaaacgtt attttaatga attagcttta aagatttgtc aagaagaagt
     6181 gatatgagtt gataaaagtt ttgacgaatt acttttttta gcgtaaaatg gtagagaatg
     6241 ataaaataag gagaactgcc ttgaaattag acgtatttgc aggacaagaa aaaagcgagc
     6301 tttccatgat tgaagtggcg cgtgccattt tggaagaacg cggtcgcgac aatgagatgt
     6361 actttagcga tttagtcaat gagattcaaa attacttagg aaaatctgat gcaggtattc
     6421 gtcatgcatt accatttttc tatactgact taaatacgga tggctcattt attcctctag
     6481 gtgaaaacaa atggggactt cgttcgtggt atgctattga tgaaattgat gaagaaatta
     6541 ttactctaga agaagatgaa gatggcgcac aaaaacgtaa gaaaaaacgc gttaatgctt
     6601 tcatggatgg agatgaagat gctattgatt atagagatga tgatccagaa gatgaagatt
     6661 tcacagaaga atcagctgag gttgaatatg atgaagaaga tccagatgat gaaaaatcag
     6721 aagtggagtc atatgattca gaattgaatg aaattatccc tgaagatgat tttgaagaag
     6781 ttgatatcaa tgaagaagac gaagaagatg aagaagatga agaaccagtt ctctaatgtc
     6841 tttagttcag aaataaaaat gtaattatca tttgaca
//
LOCUS       DAUCWU010000076         6817 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUCWU010000076 DAUCWU010000000
VERSION     DAUCWU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.076"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(359..667)
                     /locus_tag="VGY24_001430"
     CDS             complement(359..667)
                     /locus_tag="VGY24_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284401.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNLSI
                     DTPLLKVRGESLAPQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVRID
                     "
     gene            complement(733..1185)
                     /locus_tag="VGY24_001431"
     CDS             complement(733..1185)
                     /locus_tag="VGY24_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ4284402.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFSYLFGRTTSGENVDEFKAM
                     QTTAVYACVRILAEAVASLPIHIYERTENGKEKKLDHPLYFLLHDEPNPEMSSFIFRE
                     TIMIHLLIWGNAYVQIIRDKSGMVSLLLKQKVSVLIRKLLLIIWQRMP"
     gene            complement(1290..1562)
                     /locus_tag="VGY24_001432"
     CDS             complement(1290..1562)
                     /locus_tag="VGY24_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284403.1"
                     /translation="MELVKTIQIGDNIYLPIPDPFGIQENQEFNLYKSTDGTLVLSPS
                     DSKLSADHQSLSSDEQMTTVVEQARLDEIATSVLSRHLDAFKELTE"
     gene            complement(1581..1886)
                     /locus_tag="VGY24_001433"
     CDS             complement(1581..1886)
                     /locus_tag="VGY24_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ4284404.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQAM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(1886..2188)
                     /locus_tag="VGY24_001434"
     CDS             complement(1886..2188)
                     /locus_tag="VGY24_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ4284405.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLSQLKENA"
     gene            complement(2244..2420)
                     /locus_tag="VGY24_001435"
     CDS             complement(2244..2420)
                     /locus_tag="VGY24_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ4284406.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            2436..3329
                     /locus_tag="VGY24_001436"
     CDS             2436..3329
                     /locus_tag="VGY24_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284407.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILISAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHMATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            3329..3553
                     /locus_tag="VGY24_001437"
     CDS             3329..3553
                     /locus_tag="VGY24_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284408.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            3544..5202
                     /locus_tag="VGY24_001438"
     CDS             3544..5202
                     /locus_tag="VGY24_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEQ4284409.1"
                     /translation="MLIEMWSPVFKKHGEIREPICFHHGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPTYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNRLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTQQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTEIQQYFPDTNLKKLYEVEAYHKKLATILDSEFSTERESLIAEIDEL
                     ESQLTILNQELQELGNIPNLSSEYLENYSKLTATINALKEQSEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREVNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALAHDSLLLSNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            5192..5401
                     /locus_tag="VGY24_001439"
     CDS             5192..5401
                     /locus_tag="VGY24_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4284410.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(5504..6712)
                     /locus_tag="VGY24_001440"
     CDS             complement(5504..6712)
                     /locus_tag="VGY24_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4284411.1"
                     /translation="MNNRKKNIQYLVYSKVIYRIGDVMFDFANNTFLAGLNPASLSLV
                     AVYQSLESVIGVLFNLFGGVIADSFKRKKVIITTNILCGTACLVLSFLTKEQWLVYAI
                     VLTNVILAFMSAFSSPSYKAFTKEIVKKDGISQLNSLLETTSTVIKVTVPMVAIFLYK
                     LLGIHGVLLLDGLSFLIAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHK
                     SIFIITVLSALVNFFLAAYNLLLPYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGF
                     VNKELSSMRLILFLSLSGLMLMLAPPFYIMFHNAIILALSPALFSLFLSIFNIQFFSL
                     VQKDVDNDFLGRVFGIIFTITILFMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVF
                     RILFKKYNIR"
BASE COUNT         2315 a         1358 c         1100 g         2044 t
ORIGIN      
        1 ttcttcaagc gataaaacaa gcgataaact attacaattt attttaaatt ctttaacgat
       61 tactaacttt ttaacgttat tattttcaaa tactctacat aaaattaccc tagagtatta
      121 aaactcccac tcagactcct agtccctttg tttcaagcta tttagaactt ttattgtatt
      181 atccacttta ttcctatttc tgttgctatt agttttatca tacaatctaa cctttaccct
      241 ttttagggag tgcaaaattc aaatgaatcg tgcatctcaa ctcccaatat aacttctttc
      301 attataccac agataaacta aaaaaggacg tgccgaattg atacgccctt gccttttatc
      361 aatcaatccg tacgccattt ttgaaggtta ggctgatgtg cttgtctact tttatatcca
      421 acctttcaac acttgtctgc cagagttctc cctttggacc aggagttaaa gagatggttg
      481 taaggtcagc ttgtggcgct agactttccc ctctcacctt taggagcggt gtgtcaatgg
      541 aaagattccc atccctatcc caactaaaga ctggagtatt ctgtcctgga ataacgacct
      601 tatcagcttc aacttctagg ctcttcacat actctgatag aatctgttgg gagataagcc
      661 ttgtcatcca tgctgaacta gagacggtca actcatcaat cttagctttt tgaagtgctg
      721 caactcgtgc ttctaaggca tccgttgcca gataatcaag agcagctttc ttatcaaaac
      781 gctcaccttc tgctttagca gtagcgatac cataccactt ttatctcgta taatctgtac
      841 ataggcattt ccccatatca aaagatggat catgattgtc tctcgaaaga taaaggagga
      901 catctctgga ttgggttcat catgaagaag aaagtacagt gggtggtcca gctttttctc
      961 cttcccattt tctgtccgtt catagatgtg aataggaagt gacgccaccg cttcagcaag
     1021 aatacgcaca caggcataga ctgccgtcgt ctgcatagcc ttaaactcat ccacattctc
     1081 gccactagtc gtgcgaccaa agaggtagga aaagtcctga ccttcataac tgttttgggg
     1141 cttatctcta gccctttttc ttccaattaa atcaagtagt cccataagtc ctccttattt
     1201 tggggtacaa aaaaagcacc tcctttgaag tgctttcggt atatttcttt caaagtaatc
     1261 aacagtcttt cttacaaaac ctagtattct cattctgtca attccttaaa tgcgtcaaga
     1321 tgtcgtgata atactgaagt cgcaatctca tctaaccttg cttgttcaac aactgttgtc
     1381 atctgctcat cactgcttaa actttggtga tccgctgata gtttgctatc agatggactt
     1441 aataccaaag ttccatcagt tgatttataa aggttaaact cctgattctc ttgaatgcca
     1501 aatgggtcag gtatcggaag atagatgtta tcaccaattt gtatcgtctt tacgagttcc
     1561 atacatcact ctcccttact ttaccaatta ttcatatgac gtgatccata gacgaccgca
     1621 acaattataa cttcatcttc cagaacatga tatacaatgc gatatttttt gacaatcagt
     1681 tgtctgaagg tatagccttt tccaatcaga tcctcaatga tggcacaacg ttcaggaaaa
     1741 atggatagtg ataacattgc ttgggataac ctctcaagaa gattatccgc tgcctgtggt
     1801 gcacagagtt catcacgaac atagtgatag atgctcagca aatctgcttt agcatcatcc
     1861 gacatggtga cctgatactc tttcattaag cattttcctt caattgagat agaaattctc
     1921 tgacatcctg gcggttgcca gttttcgcat cttcaaagct ggtgataagc ttatcataaa
     1981 acttctcctg actcatgtaa tcaacattaa ctcgttgagg cgcttcaggt agagaaacat
     2041 caaatgggat acccccagtc aaaataatct gattcaaaaa catatcaatc gcagttgaca
     2101 taggaatacc taagcgtttc aaaatatcat ctgccgcact ttttactgaa tcatcaactc
     2161 gtaaattcaa agttcccgtt ttagccatgg ttatcctcct ttttttgtaa cgacattgta
     2221 tcacattttt ggagttgact caactaaaaa ctcaatatcc ctcgctcatc atagacgcta
     2281 gcttcatcac cttggcggcg aatgcaacgg tcaagcccca ggataagacc tacaataccg
     2341 tcaatcttct caacagattt ttccttatct ggcttgatgt tgcctccagg ggcttgtcgc
     2401 atcaccacgt tttgtcccat ccatttaagg actggatgac caccatgtta gattttccct
     2461 tccatcatga gtttatagag ttccttggac ggtggactca tatccttata actctgctca
     2521 aaaggcacca tggtcaagcc gacaccgcaa ttccaaggta tcttctagta accatcagag
     2581 agaagacagg gattgtaaaa tcaaccactg tgcaaaatac tgcgttcact acccagtcga
     2641 atcagctcaa aaaacaatac gggcagttct tgttaacaga atctaataac tgttgtgcat
     2701 gcccaggctg tgatagacca ctcattctaa ccagtggcaa cttagcagca gaaaattacg
     2761 aggtttcagc cattgaaaaa gataaggacg ctgaaccact gaatctgata gccctctgcc
     2821 cagattgttt tttaacctat caagcagata gtcgcaaaaa aattgtaaca gctctaaaaa
     2881 atgttaaaaa gattctaatc tctgcacata acagccaaca agcgatttct gatatgaagt
     2941 tagacagtgg aattgtttca gttttgacta gtctcaataa actaaaatgt gatgaatatg
     3001 atatctccta tgactcgaag caattgactg ataagatatc acctgagaac aatcgtacgc
     3061 tttatcaatt ggttaaaaac ccagttattg ataactactt aaccattcaa aaaatcatcg
     3121 taaatttgga caaacaagga aaaattgatt acgaggaaat ccaatatcaa acgcgatcga
     3181 tgtataaaaa gttaaaagca gccaagcata ataatctggc tattttcaat actatttcgg
     3241 aaaaactcca tatggctaca ctacaggaca tctacttctg tcagacgatt gtctcttact
     3301 ttatccaaaa atgcgaggta cttgaataat gcaattacca aataaacttt actcctatca
     3361 aaaaagcacc cttgcttatt tgcctagagt gctaaatgaa atcaaaagtg gaaactcaaa
     3421 cgtcaaagat atctttcacg ctatctcaga agagttggat gatcctacag atttcctatc
     3481 tataatggat tgcttgtatg cattgaacgc tattgaaatg tctaatgaag gagaagtaaa
     3541 attatgctta tagaaatgtg gtctccagta tttaagaaac acggagaaat tcgggaacca
     3601 atctgcttcc accatgggtt aaacgtgatt atggggatgg atttagctga taactctatc
     3661 ggcaaatcct cctcactatt agccattgat ttcatctttg gaggtaatag ctatctaaaa
     3721 tcaattgcgg ttaaaaaatt aggtgatcat cctacctact tctgctttca atttgagaaa
     3781 aagttttatt tttcaagaga tacggccaac ccagatatca ttctagtgtg tgacaagagc
     3841 tactctccaa ccggtgaaac aatggaccta ggaatttttc ttaacagact taagaaacga
     3901 tatcatttag attctccaga gctatcattc cgattagcta tgagtggctt ctttaggatt
     3961 gcaggaaaaa gtaatcaaaa taccgatttc cccttacaag tttattcgag ccaaaaatct
     4021 tctgaatcta ttactactct catccaactg tttaatttgt atgataacat tgcacgatac
     4081 aaagaacgtc tgaaagataa gagcgaccag ttaacaactt ttcgtaatgc tcgaaagtat
     4141 accttcatct ccaacctggt tggcggtaaa aagcaatttg aagcaaatgt ctcagaaata
     4201 aaaagattgg aatatgattt agcacatctc caagatactc agcaagataa aattgactct
     4261 gatgatattg agaaaaatca acaaaaactc caacttagaa atactaagct ggagttagaa
     4321 agcagtcttc gggacaagca aagacgtctt aagctattgg acatcagtat cgaatttggc
     4381 ttatatccta ctgaatctga cttaacagag attcaacaat acttcccaga tactaatctc
     4441 aaaaaattat atgaagttga agcttaccac aaaaaattgg caactatttt agattccgaa
     4501 ttctcaactg aacgtgaatc cctaatagct gagatcgatg aattagagag tcaattaaca
     4561 attctaaacc aagaacttca agaactagga aacatcccaa atctttcaag tgagtactta
     4621 gaaaactact ctaaactaac agctacaatc aatgcactaa aagaacaaag cgaggcctac
     4681 ttaaaggagt ctggtctatc caaagaaaaa tcagaagcgg acgctgactt gaaacgcagt
     4741 acagaagata tccttctaga tctagaagta aaaatcaaca gtaagatgcg tgaagtcaat
     4801 aacattctat atccagatat tcgcaaagct cctcaaatta acctcaatgc tcacaatagc
     4861 tactccttct atacaccaga tgatgatggt acaggaacta agtttaaagg gatgattgta
     4921 ttcgacttag taatgctcta ccttactaat ctgccagcac tcgcacacga ttcattactc
     4981 ttgagtaatg ttagctacca agcgactgaa gcacttttga agctgtatga ccagtcaagg
     5041 tcattgaaca aacaagtctt tttagccttc gataaagcca gctcttattc tccagatgct
     5101 aatcaactct tgtcagaaaa tacggtattg agactctcta gtaacggaaa tgaactctac
     5161 ggtatttcat ggaacaaagg agaaaactca gatgagattt agttacaata aattatggaa
     5221 acttcttatt gataaaggtt ggaccaaaac aaagctcagg caagaagctg ggattagttc
     5281 ttccacgatc gcaaaattgg gaaaagggga aaatatcact actgatattt tattggaaat
     5341 ttgtattgca cttgattgta agattgaaga tatagtagaa attgtagata ataatatcta
     5401 gaaataaaaa aagcactaag ttgataagat gtgtaagtca gatcaactta gtgcctttac
     5461 tttgaaaaaa aataattttc tcaataatac ttagtactaa ctgttaacga atattatact
     5521 ttttaaataa tattctaaat actaatgaca atatagtaat gcacgaacca ataataaaaa
     5581 gattaaagct attgttagga tttaatgcca ccgagaaaaa gccagtccca ataggcataa
     5641 aaagaatagt tattgtaaag ataataccaa acactctccc taaaaaatcg ttatcaacat
     5701 ctttctggac aagagaaaaa aattgaatat taaagatgga aagaaataaa ctgaataaag
     5761 ctggtgacaa agctaaaata attgcattat gaaacataat ataaaatggg ggagctaaca
     5821 ttaacatcag tcctgataaa gatagaaaca atatcaatct catacttgat aattctttat
     5881 tcacaaaacc acttaatatt gccccaataa atccaccaat tgcttctgct gttaaaaaag
     5941 tgccataaag cccagttgaa atttctccaa acatttgatt actatagggc aacaataaat
     6001 tataagcggc tagaaaaaaa ttaacaagtg cagaaagaac ggtaataata aagatggact
     6061 tatggctgta aacatattta aatcctattt ttaaatcgtt aaatatttct ctgattgtca
     6121 ccttctcctt tatcaccact tcgtcattaa caggtaaaat aaaggaaatt agtaaagcag
     6181 ctattagaaa tgatagtcca tccaatagta atacaccgtg tataccaagt agcttatata
     6241 agaaaattgc taccatagga actgttactt tgattacagt acttgttgtc tctagcaatg
     6301 aattaagttg tgatatacca tcttttttaa caatttcttt tgtaaatgct ttataggatg
     6361 gactagaaaa agcactcata aatgccaaga taacattagt tagtacaatg gcataaacca
     6421 accattgctc ttttgttagg aacgagagta ctaagcaagc tgtgccacat aagatatttg
     6481 tagtaataat aacttttttc cgcttgaaac tgtctgcaat gactccacca aataaattaa
     6541 atagaacacc tatgacactt tctagagact gatatactgc aactaatgat agagacgctg
     6601 gattaagccc agcaagaaaa gtattgttag caaaatcaaa cataacatcg ccaattcgat
     6661 atataacttt actatacact aaatattgaa tatttttttt cctattgttc attttaaaaa
     6721 cctttaaaat tttcagatat aataactttt aactgaattt attacttaaa aatatttttt
     6781 cgcaaaaatg tttttgttaa tcatactgaa aaatatc
//
LOCUS       DAUCWU010000077         6720 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUCWU010000077 DAUCWU010000000
VERSION     DAUCWU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.077"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            583..1059
                     /locus_tag="VGY24_001441"
     CDS             583..1059
                     /locus_tag="VGY24_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ4284412.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            1117..2298
                     /locus_tag="VGY24_001442"
     CDS             1117..2298
                     /locus_tag="VGY24_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ4284413.1"
                     /translation="MPLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            2288..3535
                     /locus_tag="VGY24_001443"
     CDS             2288..3535
                     /locus_tag="VGY24_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ4284414.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLATLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(3594..5246)
                     /gene="fbp54"
                     /locus_tag="VGY24_001444"
     CDS             complement(3594..5246)
                     /gene="fbp54"
                     /locus_tag="VGY24_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ4284415.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRLQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAEHDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSMVPNNQDSVVLDN
                     YYTGEKLEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            5600..6598
                     /locus_tag="VGY24_001445"
     CDS             5600..6598
                     /locus_tag="VGY24_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ4284416.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANQQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
BASE COUNT         2012 a         1162 c         1387 g         2159 t
ORIGIN      
        1 tattaaaaat agataaataa agtaaaaatt accaagatat atttttagtg ttttaaaatt
       61 atgctaatat ttattgatat tttaatataa aatatatgag gagataagag attgggaggt
      121 tgtgacaatc gagcggatat cagatgttat ggcagaactt tctgagtctg aataatttta
      181 gttcaccaaa gaatcaagaa agaaccattg gggcaaaatt ggggcataag tttagaactt
      241 ttgtcgtttc ttgtgtcgat acaataccgg tttaatacta acaaatgctt ttattatcga
      301 ctttgtaacc attattgttc cttgtgtttt ttcgggaata aactcaagaa gtgattaaat
      361 aaaacattaa agaaccttgt catatcaatt atcgttgata tgacaaggtt cttttgatag
      421 tttaaattga tgtttgtcat tgtaggctct tatctacact tttttaaggc atctttagaa
      481 ggttttgagc gtgactaagt attcacactt tgactcataa aatgatagca tattttctag
      541 agattggtat aatagaaaca aagatggaaa ggaaagagat agatgactca attagcaacc
      601 atttgttaca ttgataatgg cgattctttg ctattattac accgcaataa aaaagaaaat
      661 gatgtccaca aaggcaagtg gatttcagtt gggggtaagc tcgaggctgg tgagacccca
      721 gatgagtgtg cccgtcgtga gattttagag gaaacccatt tgactgttac agagatggcc
      781 tttaaaggaa tcattacttt tccagaattt acccctggtc atgactggta tacctatgtt
      841 tttaaagtaa caggatttga aggagacctc atttcagatg aagagtcacg agaaggaacc
      901 ttggaatggg taccttacga tcaagtgttg gaaaagccaa cttgggaagg tgattatgac
      961 atttttaaat ggattttaga agaccgtcct tttttctctg ccaaatttac ctatgaccaa
     1021 aacaaccaac tcatggataa atctgtgacc ttttattgat ttacccaggt taccagtaaa
     1081 acttatcatt ttcccaaaga aaggaaccct atcttaatgc ccttaatacc tgaaaagtct
     1141 agaacagata gcttatttta caaatggttt ttaaataatc aagctaccat ggctttagta
     1201 ataacacttt tagccttttt aacgattttt gtttttacta aaatctcttt tttatttatg
     1261 ccagtgattt ctttttttgc ggtcatcatg ttgcccttgg ttatttcgac cattctgtat
     1321 tatctgacca aacccttagt tgaccttatt aatcacttgg gcccgaatcg taccacctct
     1381 atttttattg tttttggttt gattacctta ctttttgtct gggcgatttc aggctttgtt
     1441 cccatggtac agacccaact taccagtttt attgaggatc tgcccaaata cgtgggcaag
     1501 gtcaatgaag aagccaataa acttcttgaa aatgaatggt tagtcagcta caagccccaa
     1561 ctccaagaca tgcttaccca tacaagtcaa aaggctttgg attacgcgca aagtttttca
     1621 aaaaatgcta ttgactgggc tggtaatttt gctggtgcca ttgctcggat taccgtggct
     1681 attattattt cgccctttat tctcttttat tttttacgag acagtagcca catgaaaaat
     1741 ggccttgtga atgtcttacc tctcaaatta cgggtgccta tggttcgagt cttgggagat
     1801 attaacaagc aattatcagg ctatgtccaa gggcaggtta cggttgcaat tgttgtcggc
     1861 tttatgtttt ctatcatgtt tagccttgtg ggactgaaat atgccatcac ttttgggatt
     1921 attgctggct ttcttaacat gataccttac ttaggaagtt tccttgccat gatacctgtt
     1981 gtgattatgg ctatggtaca aggcccattt atgttggtta aggtgcttgt tatctttatg
     2041 attgagcaaa ccatcgaagg gcgttttgta gcaccgcttg ttttgggaaa taagctgagc
     2101 attcatccta ttaccattat gttcttgtta ttgactgcag gctctatgtt tggtgtctgg
     2161 ggtgttttcc ttgtcatccc aatctacgct tctgtcaaag tcgtgattaa agaattattt
     2221 gactggtaca aaaaagttag cggtttgtat gacgaagaag tattggtgat tgaggaggtt
     2281 aaggaccatg ttaaatagtg aaaaaatgat tgcttccttg gatcagcaag acctagcgca
     2341 cgctgaaaaa tatttccaaa aagctttaaa agaagatgat gcagatagtt taatcgcctt
     2401 aggagaatac ttagaaagta taggcttttt gccacatgcc aagcgtattt atttgcagtt
     2461 agctgatgac tacccagaac tcaatataaa tttagctcag attgcagcag aagacgacgc
     2521 cattgaagaa gctttcttgt atttggataa ggtttcaaaa gatagcccca attatttgtc
     2581 agctctttta gtcatggctg acctctatga catggaagga ctgacagagg tggcgcgtga
     2641 aaaattgctt caagcagtgg ccatcagctc agagccattg gtcatttttg gtttggcaga
     2701 aattgacatg tccttgcagt attttaaaga agctattgat tactatgcac agttagacaa
     2761 tcgtcaaatc cttgaattga caggtatttc cacttatcaa cgcattggac gagcctatgc
     2821 tagcttaggg aaatttgagg cagccatcga attcttggaa aaggccgtgg ccattgaata
     2881 cgaagatgaa actgtttttg aattggcgac cctcttgtat gaccaagaaa attatcaaaa
     2941 agccaatctt tactttaaac agttggaaac catcaatcca gattatccag gttatgaata
     3001 tggttatgct ttgagtcttc atgaagagca taagacgtca gaagctttgc gcttggttca
     3061 acagggccta cgtaaaaatg catttgacag tcaattatta ttactagcat cgcagctttc
     3121 ttatgaatta cacgataggc agaatgctga aaattacctg cttcaagcaa aggaagtagc
     3181 tgttgatgac gaagagattc tcatgcgttt agcgaccctt tattttgatg ccgagcgttt
     3241 tgaggaggtt attgctctta atcgtgagac gattgataat gtcttaacca aatggaccat
     3301 tgcaaaggct taccatgcct tggaacaaga agaagttgcc ttggctcttt ataatgagat
     3361 ttcggctgat ttggcagaaa atccagagtt tttacaggat tatgcctatt tattaaggga
     3421 gtttggccag tttcataagg ctatccaaat ggctaccgct tacctcagac aagtgcctga
     3481 tgatgtgaat atgcaagact ttcttgacca tatcaagcac caacaatcag aataaaaaaa
     3541 cagttctcct aggggaaata actttcctag gagagctgtt ttttagttgc ttatcaggat
     3601 aatttcatag agagaatttt ggcttggtca ggagtaaccc gtaaggtttt ttgaccagta
     3661 taagtcacaa atcctggctt ggcgccacta ggtttgtgta attttttagc ttcaatcata
     3721 tcgacctgaa cgagatttga taacctagct ttcgaataat aagcagctag ttcggcagca
     3781 tcagttttaa cctcgtcact tgggtcaaga ttgtctttga tgatgacatg acttccaggg
     3841 atatccttag catggaacca gagttctcct tttttagcca ttttaaaggt gagttcttca
     3901 ttttgcaagt tattgcgtcc caccatgaga atggtttttc catctgaagc caggtactgc
     3961 tcaggctttt tacgtttatg gcgtttgtct ctttggcgac ttttcaaaaa gccagcctga
     4021 tataattctt cgcgaatatc ttcgatgtcg tcgatacttg cttgagataa attgtagtcc
     4081 acgctctcaa agtaagtaat actttgcttg gtatcggcaa tcaatccaga taagtgtttg
     4141 acagcttctt ttagcttttg gtatttttta aaataacgtt gagcattttg atttggtgtc
     4201 agagccttgt ctaaggcaat ctcaagcttt tcacccgtgt aataattgtc taataccacg
     4261 gagtcttggt tattaggaac catactcagg taagtggtta acagctcccc cttttgacgg
     4321 aataattcag cattttcagt ggctagcagc tcagcttctt gcttacttag cttgttgcga
     4381 tttttgtcca gttcagtttg cacacggtga atcaaatcac tggcttgctg attgatacgg
     4441 tcatgctcag ccttatcttg ataaaagtgg tcaagcatgt cagataaagt ctcaaacgtc
     4501 gcatggctat ctgagaaaag aacaggcgca aaagaagctg tggtcagatt tgcctgagta
     4561 ggtctagcaa aaaactcacg gaatcgtttt aatttgtctg tggtcagaag ctcggccaat
     4621 tcttttgcgg tgtctcgccc taagccttga aaatgctgtt gcaagctttt aactgttagt
     4681 tcttgtgttt ggaggatttc aaatagagga acatctgtaa ttgtaaaagg attaacagca
     4741 gctgttttag gaggctcaat gtaggtagag ccaggtaaaa tggtgcggta agagttttgt
     4801 gaaaagccaa catgtttaat ggattcgatg attttatttt ctgcacggtc caccaagatg
     4861 atattactgt gcttccccat gatttcaatg attagggttg cctgaatagc atctccgatt
     4921 tcatttttat tagatacctt gatttcgata atgcggtcat tatcaatctg ttctaactgc
     4981 tcaatgactg caccctggag gtatttacgc ataatcatgg taaaggtatt aggtacctga
     5041 ggattttgaa agtccgcttg ggtaatttga agtcgtccaa aaacggggtg agcagacaat
     5101 agcaatttat aattcttgcg atggttacga atggttaaga caagttcccg ttcaaagggt
     5161 tgattgacct tttggatacg accgtaaagc aagttctctt ttaattcgtt ggttaaatgg
     5221 tgtaagaaaa agccgtcaaa agacatggtt tctcctttct gaattctaat ctcttcatta
     5281 taccacaaat acttgactaa aaatatggac gaaagagctt gcaatttttt tgatttaggt
     5341 tataatgtta aaaaaacgag aaaggaagtc aatgatggta aaaacacaac acttagcaaa
     5401 ttggcaaagc tggcatagat aagttgtgtt tatgaattgg catagatacg acttttagag
     5461 tacttctaaa aagatatcta tgcgctgaca ttggatttca caagagcgcc tagtaatcat
     5521 tactaagtgc tttttctttt gagaagtatt ctcatcatca acgttttaaa gagattgaca
     5581 tgaaatgtga ggaattcaga tgaaaaacaa gagtttaata gctaccctcc ttgttttaac
     5641 tgtaatagtg ataggctctc tattatcaaa aggagtatca aaggaaaacc gtgatttggc
     5701 taaccaacaa aacattacaa ttggcatctt acaattcgtt actcatgagg ctcttgatga
     5761 tattaagcga ggtattgaag atcaactcaa aaagcagatg cctcaaaaac aaaatgtagt
     5821 gattaaggtt atgaatgctg agggggatca gagtaaaatc caaacaatga gtaggcaatt
     5881 agtgcaatca ggaagtgata tcgtgattgg tatcgcaacc ccggcggcgc aaggcttagc
     5941 agctgccagc aaggatatcc cagttgtcat gtcagcagta tctgatccag taggatctag
     6001 attagtgatg caattggatc aacctgaagc taatgtaact ggtctatcta ataaagtgcc
     6061 tgttaaacag acaattgatt taatgaaaaa gctgacacca catgtcaaaa cagttggaat
     6121 tttatatgca agtaatgagg ataattcctt atctcaagtt aaagagttta ggcgattagc
     6181 tcgcaaaaag ggctatcaag ttatttcata cgcggttcct tctactaatg aagttcctgc
     6241 cacgatgtca gttatgttag gtaaagtaga cgcggtattt atcccccaag acaatactat
     6301 agcaagtgca ttttcatcag tcatgacaac cagtaaggct gcaaagattc ctgtctacac
     6361 tagcgttgat cgaatggttg aaaaaggtgg tttggcagct atttctcaaa atcagtacga
     6421 tttgggagtt caaacagcta atcaggtgtt aaaattaatc aaaggaaagc gtgttgtaga
     6481 tgttcctgtc aaagttgtgg atattggtca accgctaatt aataagaatg tagcagcaga
     6541 attaggtatt gctattaaca gggaagattt tccttcagct tcttttattg agaattaata
     6601 caatgacatg tgatataaaa ggtcaagaaa aaaccataaa aaacaccctc actgagatga
     6661 atttggatgc caagtcagac aatcgtcttg aggatgtaac ggttattttt atcattttta
//
LOCUS       DAUCWU010000078         6694 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUCWU010000078 DAUCWU010000000
VERSION     DAUCWU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.078"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            83..1327
                     /locus_tag="VGY24_001446"
     CDS             83..1327
                     /locus_tag="VGY24_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ4284417.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSEDYNKNIIERIYTSSYIDSSYSIKNWIKGVNEVN
                     KADIIKVANLLKLQTVYFLEGI"
     gene            1329..2618
                     /locus_tag="VGY24_001447"
     CDS             1329..2618
                     /locus_tag="VGY24_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ4284418.1"
                     /translation="MTKLVKINYPNIDEDLYYVKLENGLTVYFIKKIGFLEKTAMLTV
                     GFGSLDNKLTVDDESRDAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQ
                     TSFFFSTASKFQENLELLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGI
                     LQNLFPKTSLANDIAGSKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLA
                     IQRFQTTLSYPDRKRVTVDPLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLT
                     YRIALKLFLSMLIGWTSKIYHTLYEDGKIDDSFDVDVEIHHNFQFVLISLDTPEPIAM
                     SNYIRQKLATIKISKEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKET
                     YFDIPKIIERLTLKDVVTIGKAFFEKADASDFTVFPK"
     gene            2668..3693
                     /locus_tag="VGY24_001448"
     CDS             2668..3693
                     /locus_tag="VGY24_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ4284419.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTMSPVSQFKSNNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            3707..4249
                     /gene="pgsA"
                     /locus_tag="VGY24_001449"
     CDS             3707..4249
                     /gene="pgsA"
                     /locus_tag="VGY24_001449"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ4284420.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVVSNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            4251..5090
                     /locus_tag="VGY24_001450"
     CDS             4251..5090
                     /locus_tag="VGY24_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ4284421.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVHMLQEEGYPIDYGYLTEKELENQLCQLI
                     SKM"
     gene            5066..5908
                     /locus_tag="VGY24_001451"
     CDS             5066..5908
                     /locus_tag="VGY24_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ4284422.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            5901..>6694
                     /locus_tag="VGY24_001452"
     CDS             5901..>6694
                     /locus_tag="VGY24_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ4284423.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTKFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTS"
BASE COUNT         2235 a         1047 c         1210 g         2202 t
ORIGIN      
        1 aagtcaaaaa aagttatcac tcacctaacc tgaataaaaa tggtataatc gtaaggatca
       61 cactatttat taaggaaaca ttatgaaaat tgttcaggga gtacaacttc atctgattaa
      121 gaccaaacag tttaaaacca atcatattac ctttcgtttt tcaggtgatt tgaaccaaaa
      181 aacagtagca aaaagagtct tagtggcaca gatgttagca acagctaatg agtgttatcc
      241 aactgttagg cagtttagag aaaaattagc acgactttat ggagctagct tgtcaactaa
      301 cgtgttaaca aaagggttag ttcatattgt ggatatcgat attactttta ttcaagatag
      361 atatgcttgc aatggggaaa aaatactaga tgaaatgatt cagtttttaa aagatatctt
      421 gttctcaccc ctattatcca tcgcccagta tcagcctaaa gtttttgaga ctgaaaaaaa
      481 taatttgatt aactacatcg agtcagatag agaggattct ttttactaca gctctcttaa
      541 ggtaaaagaa ttgttttatt gtaataagaa tctacaaatg tcagaatatg gtagcccaga
      601 actaattgca aaagagactg cctatactag ctatcaagaa tttcataaaa tgctcaatga
      661 agatcagatt gatattttca ttttaggaga ttttgatgat tatcgcgttg ttcagctaat
      721 tcatcaattt ccacttgata accgaaataa aaacctaaat ttctttcatt tacaaaattc
      781 tgtcaatatt attaaagaat ctattgaaaa aagagcagtt catcaatcta ttttacaatt
      841 agcttatcac ttcccttcag tttttggaca acgtgactat tatgctttag ttcttttaaa
      901 tgggttgtta ggctcttttg cacattcccg tctctttata aaaattagag aagaagaagg
      961 acttgcttat agtatagggt gtcgttttga tagttataca ggtttatttg aaatttatac
     1021 tggaattgac agtcaacatc gcactaaaac gttacaacta atcatccaag aattaaatgc
     1081 cattaaaatg ggacgattct cagaacaatt gattaaaaag accagatcga tgttgttgaa
     1141 taacgcctta ttatcagaag attataataa aaatataatt gaaagaatat acacatcttc
     1201 ttacattgat tcttcttatt ctattaaaaa ttggattaaa ggtgttaatg aagttaataa
     1261 agcagatatc attaaagtag ctaatctttt aaagcttcaa actgtctatt ttttagaagg
     1321 aatataaaat gacaaaatta gtaaagataa actatcctaa tattgacgaa gacctttact
     1381 atgtaaaatt agaaaatggt ttaactgttt attttataaa aaaaataggg tttttagaaa
     1441 aaacagctat gttgacagta ggtttcggtt ctcttgacaa taagttgaca gtcgatgacg
     1501 aatcacgaga tgctccagca ggtattgcac actttttaga acataaactc tttgaagatg
     1561 aatcaggtgg agatatttca ttaaagttta cacagctagg agcagagaca aatgccttta
     1621 caactttcaa ccaaacgagt ttcttctttt caacagctag taaatttcag gaaaatttgg
     1681 aactccttca atattttgtt ctttctgcta atataacaga tgaatctgtt agtagagaaa
     1741 aaaaaataat cgggcaagaa attgatatgt atcaagatga tgctgactac cgtgcatata
     1801 gtggaatcct tcagaactta tttccaaaaa caagtttagc taatgatatt gctggctcaa
     1861 aagcatccat tcaaaagatc acgaaaatat tattggaaac gcatcatact tatttttacc
     1921 aacctactaa tatgagtcta ttcattgtcg gtgatattga tattgacgaa acttttttag
     1981 ctattcaaag gtttcaaacc acattatcct atccagatag aaaaagggta actgttgatc
     2041 ctttacacta ctatcctgta attaaatcat cttctgttga catggatgtt actactgcca
     2101 agctggttgt tggttttcga gggtatctga ccttaactca gcattcacta ttaacgtatc
     2161 gtattgcctt gaagctattt ttatcaatgc ttataggttg gacttctaaa atctatcata
     2221 cgctatatga agatggtaaa atagacgatt catttgatgt ggatgttgaa attcatcata
     2281 attttcaatt tgtattaata tcacttgata caccagagcc tatcgcaatg tctaactata
     2341 tccgtcaaaa attagccact ataaagatat caaaagagtt tacaaatgag cacttaaatc
     2401 tattaaaaaa agaaatgtat ggtgacttta ttcagagttt agactctata gaacatttaa
     2461 cccatcagtt cagtctatac ctgtctgatt ctgataaaga aacatatttt gatattccaa
     2521 aaattataga aagactcact ctaaaagatg ttgttacaat tggaaaagca ttttttgaaa
     2581 aagctgatgc ttcagacttc acagtcttcc caaaatagat gaaaatctgt tataataggg
     2641 ttactatatt atttttgagg tacctgtatg agtgaaaaaa gtcttggtga agtgttaaga
     2701 gaatcgcgtg ttggaaaaaa tatcacacta gatgatatag aattgaagac agggatttct
     2761 tcccattact tgctggcaat ggaattggat cagtttaaaa ttattccgga agaaaaattt
     2821 gatcaatttt taaaagaata tgctgacatt gttgagttag atttcaatac tttaaaaaga
     2881 cgataccgtt atcaagttaa ttccaaaaaa gacagtgata taaaatctgt aactgagatt
     2941 gttgaagaga agttaagtaa gaaacgatta caagagaagt tgttatcaca tcagagcatg
     3001 gggtatggtc aaccgttaga agtttctaaa aacaaagaaa ccttagttat ccctgtaaag
     3061 actgttggtt tagatagcaa atcgccacta aaaacgatgt cgcctgttag ccaatttaaa
     3121 tcaaataaca ggttaattcc taaaaaaaat gtgtcacgta tgaggcgaaa tagcactgac
     3181 agtcgttcta aaacgtcact atttcccata gttatattag gaacgattgc ttttgcaatt
     3241 atcagtgtta tcattttagg tgtttggcaa caattcgaaa agagtcaaag agctaaagaa
     3301 gctgaagttg ctttgcttaa agcctctgag cagtctacta acggtttaaa aaataattca
     3361 aatgatcgca aaactcaagt cacaactgag gggtcgggta attatttaat agcgactgtt
     3421 actaaatcta aagagaccgt tgatatcagt gtgtctttaa ccgaggcaca aagtagttgg
     3481 atttctctaa caaattcaga aattggtgaa ggtggtataa cattgactca agactcgcca
     3541 acttatacag cgactttacc tgcagaaata acagaatcat tgttgacact tggtgtgaca
     3601 aaaggtgtat ctgtaacaat tgatggacaa cctgttgatc tatcaccttt aacaagtact
     3661 gacttaagtt atatcacgtt taagatccaa taattaaggt agactaatga taaaaaaaga
     3721 aaatattcct aatctattaa ctcttgttcg aatcgcaatg attcctttct ttctttttat
     3781 tacttcctca tctaataagg tggggtggca tatttttgca gctgtgattt ttgcaattgc
     3841 cagttttacc gattatctag atgggtatct tgcgcgtaag tggcatgtag tcagtaattt
     3901 tggaaagttt gccgatcctc tagcagataa gatgcttgtc atgagtgcct ttatcatgtt
     3961 agttggactt ggcttagttc ctgcgtgggt atcagctgtt attatttgcc gagaattggc
     4021 agtaaccggt cttcgattac tacttgttga aaccggagga aaggtccttg cagctgctat
     4081 gccgggaaaa atcaaaacag cgacacaaat gttgtctatt attttattac tttgccattg
     4141 gatattccta ggaaacgtct tactctatat tgctcttttt ttcactattt attctggata
     4201 tgattatttt aaaggagcaa gctttctttt taaggatacg tttaaataac atgtcagcta
     4261 ttattgaact taaaaaagtt acatttaatt accataaaga ccaagaaaaa ccaacattag
     4321 atggcgtatc gtttcatgtg aaacaaggtg agtggctgtc tatcattgga cataacggat
     4381 ctggtaaatc aacgactatt cggttaattg atggattatt ggaaccagaa tcagggtcta
     4441 ttattgttga tggagatcta ctaaccataa ctaatgtttg ggaaattcgt cataaaattg
     4501 gtatggtctt tcaaaaccct gataatcagt ttgttggagc aactgttgaa gatgatgttg
     4561 cttttgggct tgaaaataaa ggtattgcac atgaagatat aaaagagagg gttaatcatg
     4621 ctttagagtt agtcggcatg cagaacttta aagaaaaaga accagcccgt ttatctggtg
     4681 gccaaaaaca gcgcgtagct attgcaggtg cagttgctat gaagcctaaa attattattt
     4741 tagatgaagc tactagtatg cttgacccta aaggacgatt agagttaatt aaaactataa
     4801 aaaacatccg tgacgactac cagctgactg ttatttccat tactcatgac ttagatgaag
     4861 ttgctcttag tgatagagtt ttagtgatga aagatggtca agtggaatca acatcaacac
     4921 cagaacaatt atttgcaaga ggggatgaac tactccaatt aggtcttgat atccctttta
     4981 caacatctgt tgtacatatg cttcaagaag aaggttatcc tattgactat ggatatctca
     5041 cagaaaagga attagaaaat cagttatgtc aattaatctc caaaatgtaa gttatactta
     5101 tcaagcaggg actccttttg aagggcgtgc cctttttaac attaatttag atattttaga
     5161 tggttcttat acggctttta tcggacatac tggatccggg aaatcgacca ttatgcaatt
     5221 gctaaatggt ttacacgttc cgacaacggg aattgtcagc gtagataaac aagatataac
     5281 gaatcactct aaaaataaag agattaaatc tattcgaaaa catgtaggat tagtttttca
     5341 atttccagaa agtcaactat ttgaagagac agttttgaaa gatgttgctt ttggccctca
     5401 aaattttggg gtctctccgg aggaagcaga agctttagcg cgtgaaaaac tagctcttgt
     5461 tgggatttca gaaaatcttt ttgaaaagaa tccatttgaa ctctcagggg gtcaaatgcg
     5521 tcgtgttgct attgcgggta tccttgctat gcagccaaag gttttagttt tagatgaacc
     5581 gactgctggt cttgatccta aagggcgtaa agaattaatg actattttta aaaagttaca
     5641 ccaatctggc atgactattg ttttagtaac ccatctgatg gatgatgtgg ccaattacgc
     5701 agattttgta tatgttcttg acaaaggtaa aattatttta tcaggaaaac caaaaacaat
     5761 ttttcaacaa gtcagtttgc ttgaaaaaaa acagttagga gttcctaagg tgactaaact
     5821 tgctcaaagg cttgttgata gaggaatacc tatctcttct ttgcctatta ccttagagga
     5881 gctaagagag gtactaaaac atggataagc tgattcttgg tcgttatatt cccggagatt
     5941 ctctcattca tcgtcttgac ccaagaagta aattattagc gatgattatt tatattgtca
     6001 ttatcttttg ggctaacaat gttgtgacaa atttgttgat gttgactttt actttagcag
     6061 ttgttttttt gtcaaaaatc aagttgtctt tctttttaaa tggggttaaa ccaatgattg
     6121 gtattatcct cttcacgact ttatttcaga tgtttttctc acaaggagga aaagtaattt
     6181 ttagttggtg gttcattagc ataactgatt taggattgag ccaagctatt ttaattttta
     6241 tgcgtttcgt tctaattatt ttcttttcaa cgctgttaac gttgacgaca acacctttaa
     6301 gcctatctga tgcagttgaa tcattattaa aacctttaac aaaatttaaa gttccagctc
     6361 acgagatcgg actaatgctt tctttaagtt tacgttttgt gcctacttta atggatgata
     6421 ccacgcgtat tatgaatgca caaagggccc gtggagttga ttttggagag ggaaatttaa
     6481 ttcaaaaagt taaatccatt attcctattt taatccctct tttcgcttca agctttaaaa
     6541 gagccgacgc attagctatt gctatggaag ctagaggtta tcagggagga gaagggcgaa
     6601 ctaaatatcg tcaattagac tggcagctaa aagatagttt agctattgga atagtttctt
     6661 tattgggttt gctacttttt ttcttgaaaa cctc
//
LOCUS       DAUCWU010000079         6663 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUCWU010000079 DAUCWU010000000
VERSION     DAUCWU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.079"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..223
                     /locus_tag="VGY24_001453"
     CDS             <1..223
                     /locus_tag="VGY24_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284424.1"
                     /translation="TTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDE
                     LKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            238..1107
                     /locus_tag="VGY24_001454"
     CDS             238..1107
                     /locus_tag="VGY24_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEQ4284425.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1088..1822
                     /locus_tag="VGY24_001455"
     CDS             1088..1822
                     /locus_tag="VGY24_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4284426.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1855..2763
                     /locus_tag="VGY24_001456"
     CDS             1855..2763
                     /locus_tag="VGY24_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEQ4284427.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2760..3302
                     /gene="sat4"
                     /locus_tag="VGY24_001457"
     CDS             2760..3302
                     /gene="sat4"
                     /locus_tag="VGY24_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEQ4284428.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3395..4189
                     /locus_tag="VGY24_001458"
                     /note="aph(3')-IIIa"
     CDS             3395..4189
                     /locus_tag="VGY24_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEQ4284429.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4465..5037
                     /locus_tag="VGY24_001459"
     CDS             4465..5037
                     /locus_tag="VGY24_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEQ4284430.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            5010..5417
                     /locus_tag="VGY24_001460"
     CDS             5010..5417
                     /locus_tag="VGY24_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEQ4284431.1"
                     /translation="MVLNRFCRLRNEYRNFRVDRIKSICIEEELCQSHDGSLEQILKQ
                     MLSYKKLYNVILRAEKGETYNSIKNRYSLGFLEETDLGSKMEIEFQTDSFEILSKQLI
                     EYGSGIEIVQPDELKCITRKHLAQITNHCLNLI"
     gene            5528..5890
                     /locus_tag="VGY24_001461"
                     /pseudo
     CDS             5528..5890
                     /locus_tag="VGY24_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
     gene            5886..6554
                     /locus_tag="VGY24_001462"
                     /pseudo
     CDS             5886..6554
                     /locus_tag="VGY24_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
BASE COUNT         2243 a         1052 c         1486 g         1882 t
ORIGIN      
        1 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
       61 tgctagacgt attggccaga ctccacagaa tttcaataaa aaattacaac gagaaacagt
      121 aaccttggat gagttgaagg ccattgctga tgtgctaggt gtcaagtttg tgcaggcatt
      181 tattttacca gatggagaag aaataaaaat atctaacgaa taaaggagga tgtttatatg
      241 gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt tatagaaggc
      301 atcgtattag ggggctcacg tgcaagaggc acccatacag aaaattctga tatagatatc
      361 ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat tgctacagaa
      421 ttggatgata aaaatagaaa caaccttgtt gtgcctcccg gagcgtgggg tgattggatt
      481 aatggcggcg gatggttatt tataaatggg tatcatgttg acttgatttt acgtgatata
      541 aaacgggtag aacaaataat caaagatacg gagcaaggaa ttgttactgc caattatcag
      601 accgggcatc cccatggtta tataagtgca atgtatcgtg gagaattagc gattagtaaa
      661 atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga aatttaccct
      721 actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt ctctttaatg
      781 tttgtaaaag caaatgcggg ggtagaggat aaatattata ttgcaggcca tgtttttcgt
      841 attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg cattaacgaa
      901 aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata tgccgaaaga
      961 gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga tatgaccgag
     1021 aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     1081 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     1141 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     1201 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     1261 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     1321 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     1381 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     1441 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     1501 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     1561 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     1621 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     1681 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     1741 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     1801 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     1861 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     1921 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     1981 tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt
     2041 aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct
     2101 gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc
     2161 ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta
     2221 attgataaag attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta
     2281 agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct
     2341 tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag
     2401 attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt
     2461 aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg
     2521 gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt
     2581 ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc
     2641 tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact
     2701 ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag
     2761 tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg
     2821 aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac
     2881 tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg
     2941 cagattgcct tgaatatatt gacaatactg ataagataat atatctttac taccaagacg
     3001 ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag
     3061 atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat
     3121 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
     3181 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
     3241 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     3301 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     3361 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     3421 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     3481 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     3541 gtataaaggg accacctatg atgtggaacg agaaaaggac atgatgctat ggctggaagg
     3601 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     3661 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     3721 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     3781 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     3841 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     3901 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     3961 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     4021 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc gatcgatcag
     4081 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     4141 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt
     4201 agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat
     4261 ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc
     4321 tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag
     4381 ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg
     4441 gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt
     4501 cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc
     4561 ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct
     4621 gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca
     4681 gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa
     4741 tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt
     4801 gaaaaaaact atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag
     4861 aaaaatacat tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg
     4921 gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag
     4981 cctatatcac tggggtttta tgaacagaac tggtacttaa tcggttttgc aggctaagaa
     5041 atgaatatag gaattttcgt gtggatcgga ttaaaagcat ttgcatagaa gaagagctct
     5101 gtcaaagcca tgatggctct ttggaacaaa tattaaaaca aatgctttca tataaaaaac
     5161 tatataatgt aattcttaga gctgaaaaag gggagacata caattctatc aagaacaggt
     5221 attctctcgg cttccttgaa gaaaccgatt tgggaagtaa aatggaaatc gaattccaga
     5281 cagattcctt tgagatattg agcaagcagc tgattgaata tggatcaggc attgaaatag
     5341 tccaacctga tgaattaaaa tgcataaccc gtaaacacct ggcacaaatt acgaatcact
     5401 gcctaaactt gatttagaga aagcgattgg tctgttatgt attttttgta aggtttcata
     5461 tcccctgctg acatacagct gtcagtgtat tatgatattc tactatcaaa aaggaggatt
     5521 gaaaaatatg aaaccaaaga taattatgca cacacaaatt agccttgatg gccgcattaa
     5581 aggctttgat aatccggaag tttactacca ggttgccggg ggaattcatt cggatgcggt
     5641 gctgtttggg tccaatactg tttttactgc atttgaaaaa tatcctgctg aaacagaggc
     5701 tgattttgag aaaatcataa caagcccgga ggaccccaga ccaataggag tcatacctga
     5761 cagcagaggt atcttgagaa gtcttcattg cttaagaaat cttggatatc tgaaggaaat
     5821 tgtcattctg gtatcaactg ccactccgaa ggaatatttg aattatttgg aggaaaggca
     5881 ttacccatac cgagatattg gttcggggtg acagcggctt cgccacacca gaggtgtatg
     5941 agtcttgtga agcaactgaa agccagtacg ttatccgatt aaagagcaat cggaggttaa
     6001 gtcagttagc tgaacattct gttctttacg gggacaatca agaatgggaa gagcgagaag
     6061 tgcagtattt ttcactacct tatcaagcac aatcctggtc gaaacctcgt cgtgtctgta
     6121 tacgctccgt ccgtgaagga ggagaattgc tatttcatca tgcgtttatt gtgactaatc
     6181 tatccgacaa tgtctcgcct gaagtcatat tttctcttta cggcaagcga ggaacaatgg
     6241 aaaatttcat caaagaagcg aaaggtggct tttacttcga taaaacagat agcccgcgtt
     6301 tcttagaaaa tcatgtcaga atgatgatca gtgtccttgc atacaacctc gttaattttt
     6361 taaaaagcat tggttttgat aaagtgaacc agggcatgac gattcattcc atccggctga
     6421 tattgcttaa agtagccggt aaacttgttc agactgggag acaggtctat ctcaaactgt
     6481 caagttatca tgtgtatcaa actgaatttt ataaggtttt tgaacgcctg cggcgatcca
     6541 aacaatggat ttagactagt tatcacacaa attttttaac cgattctttt tcaagggatt
     6601 cgtctgccct taaatagaca aataattttt attatgatct tcttccgtag gcatcctgtt
     6661 cga
//
LOCUS       DAUCWU010000080         6600 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUCWU010000080 DAUCWU010000000
VERSION     DAUCWU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.080"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..211
                     /locus_tag="VGY24_001463"
     CDS             <1..211
                     /locus_tag="VGY24_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ4284432.1"
                     /translation="GEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFQALIR
                     GERFKGRRALLRKISPYFKEFAQQL"
     gene            792..1319
                     /locus_tag="VGY24_001464"
     CDS             792..1319
                     /locus_tag="VGY24_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ4284433.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1319..2437
                     /gene="yqeH"
                     /locus_tag="VGY24_001465"
     CDS             1319..2437
                     /gene="yqeH"
                     /locus_tag="VGY24_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ4284434.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKEMTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2461..2769
                     /gene="yhbY"
                     /locus_tag="VGY24_001466"
     CDS             2461..2769
                     /gene="yhbY"
                     /locus_tag="VGY24_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ4284435.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2838..3470
                     /locus_tag="VGY24_001467"
     CDS             2838..3470
                     /locus_tag="VGY24_001467"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ4284436.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLCMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDYYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3467..4060
                     /gene="yqeK"
                     /locus_tag="VGY24_001468"
     CDS             3467..4060
                     /gene="yqeK"
                     /locus_tag="VGY24_001468"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ4284437.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLDVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            4084..4437
                     /gene="rsfS"
                     /locus_tag="VGY24_001469"
     CDS             4084..4437
                     /gene="rsfS"
                     /locus_tag="VGY24_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ4284438.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            4485..5228
                     /locus_tag="VGY24_001470"
     CDS             4485..5228
                     /locus_tag="VGY24_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4284439.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5482..6588
                     /locus_tag="VGY24_001471"
     CDS             5482..6588
                     /locus_tag="VGY24_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ4284440.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTLSYPQKKQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
BASE COUNT         2079 a         1016 c         1431 g         2074 t
ORIGIN      
        1 aggggaggtt gcacatgcca ttccatcgag agggttatgg ctagacgact atgtggctta
       61 tcaagaggta atagctagct tagagaacca attaaattcg caagagcgta tgcagtttca
      121 agcacttatc aggggcgaac gtttcaaggg aagacgtgct ctacttagga agatcagtcc
      181 ctattttaag gaatttgcac agcagttgta agcaagacct ataaggaatt aggggtaaat
      241 ctatgaaaaa agtgtaatca ttttttgatt tacagttatt atcatgtgtt aactggcgac
      301 tgtcctgttt acaagtaagt tcacaagtat gggataaaat agtataaact atagtaaata
      361 ctgaatagtc atagaaaaat atcggcatag gtcagaagag ctgatatgtt taacactttt
      421 tgcggatatc tactgtattt aaatgtattt gatatccttg tcaaggatat acgtctaaaa
      481 aagttcaaaa cgaaatcatt tgtgattatc ttatcaggag ttgactcttg ataagatttt
      541 tgtatattag tattgtggca cgtttagata gtttggcagt gaatttatag agtgtcaaac
      601 aacgagtgtt ttgtgttaca ctaaccatta gaagagtcta ttgttgagca tatcaaggtg
      661 ttatcttttc gagttgaggt atcatatcta gttgtaatgt caaacatatc tagtatattt
      721 aactatggtt ttaaatgcta tttcaaaaaa aatatgataa aatagaaagt actaattatt
      781 ttggagatga catgagtatc gatgattata ggccgaccta tatggtagag gctatttatg
      841 atttaagagc aaatgatttg ttgcgtcatg gtattacggc tgttttagtg gatttagata
      901 ataccttaat tgcatggaat aatcctgatg gcacaccaga ggttcgcgct tggcttgatg
      961 aaatgactat cgcggatatt agcgtggttg ttgtttcaaa taataaacat tctcgtgttg
     1021 agcgtgccgt ttcacgcttt ggtgttgact ttattagccg tgctttgaag ccatttgctt
     1081 atggtattga aaaagccatt gctcgttatg gatttgatcg caacgaggtt atcatggttg
     1141 gagatcagct aatgactgat atcagagcta gtcatagagc tggaataaaa tcagttttag
     1201 tcaaaccact tgttgcttct gatgcttgga atactaaaat taaccgctgg cgtgaacgcc
     1261 gtgtgatggc aaaattagaa gagaaatatg gtaaattatc atatcaaaaa ggaatttaat
     1321 ggaagaatta ttttgtatag gctgtggtat ccagattcaa actgaagata aagaaaaagc
     1381 tggttttacg ccagctgctg ctttgaaaaa gggtatggaa acaggagaac tctattgtca
     1441 gcgctgcttt agattgaggc attataatga aattacagat gtccatatta ccgatgatga
     1501 atttttaaga cttcttcacg aagtgggtga tagtgatgct cttgtggtta atgtgattga
     1561 tatttttgac tttaatggtt ctattattcc aggtctatct cgctttattt ctggtaatga
     1621 tgtcttactg gttgggaata aaaaagatat tttgccaaag tctgttaagg atggtaaagt
     1681 tactcaatgg ttgaccgaac gtgcacacga agaaggtctt cgccctttag acgttatgtt
     1741 aacgagcgct caaaataaat atgcgattaa agacttgatt gagcgtatca atgagttacg
     1801 taatggtcga gatgtttatg ttgtgggggt cactaatgtt ggtaaatcaa ccttgattaa
     1861 tgcgattatc caagagatta ctggtaacaa ggatgtcatc acgacgtctc gttttcctgg
     1921 gacaactctt gacaaaattg aaattccttt ggatgatggg acgttcattt ttgatacacc
     1981 aggaattatt cataggcacc agatggctca ttatttatcc cctaaagagc taaaaatagt
     2041 gagtcctaag aaggaaatca agcctaagac gtatcaatta aacccagaac aaactctctt
     2101 tttaggtggt ttgtctcgct ttgactttat caatggggag cgtcaagggt ttacagcttt
     2161 ttttgacaat caactagaat tacatcgtac aaaattagct ggagcagatg ctttttatga
     2221 taagcatgtt ggaactttgt taactccccc aggtaagaaa gagatgacag catttcctaa
     2281 attagttcgt catgaattta caattgatca gaaaatggat attgtttttt ctggtctagg
     2341 atggattcgt gttaatggtc aaaaagactc aaaagcgata gttgctgcat gggctcctga
     2401 aggcgtcgct gtcattgttc gtaaagcgat tatttaaaaa taaaaaagaa gttggaaaaa
     2461 atgcttacaa gtaaacaaag agctttctta aaatctgagg ctcatagctt aaaaccgatt
     2521 gtacaaattg gcaaaaatgg attaaatgac cacattaaga ctagtattcg tcaagctttg
     2581 gatgctagag aattgataaa ggtcactttg ttgcaaaata ctgatgaaga tattcatgaa
     2641 gttgcagaga ttttagaaga agaaattggg tgtgatacgg ttttgaaaat tggtcgtatc
     2701 ttgattctct ataaggtttc tgctaagaaa gaaaaccgca agttgtcccc taaagttaaa
     2761 gcaatctagt ccttatattt gtataggggc tggtcttatg agacgaggac tattgtcgcg
     2821 agaattttgg agaaagtatg gcattagaat tgttgacgcc ttttacgaag gtggaattag
     2881 aggaagaaaa aaaagaaagc aaccgcaaac agattgggat tttaggaggg aattttaatc
     2941 ctattcacaa tgcacacctt gtggttgcag atcaagttcg ccagcaatta ggattagatc
     3001 aggtgctatt gatgcctgaa tgtaaaccac cgcatgtgga tgccaaagag actattgatg
     3061 agaagcatcg tctttgcatg ttggaattgg ctattgaaga tgttgaaggg ctagctattg
     3121 aaacctgtga acttgaacgc cagggaatca gctataccta tgatacgatg ctttatttga
     3181 ctgagcagca tcctgatgtc gattattatt ttattatcgg agctgatatg gttgattatt
     3241 tgccaaagtg gcaccgtatt gatgaactgg tcaaactcgt tcaatttgtt ggggttcaac
     3301 gtccaaaata taaagctgga acctcttacc cagtcatttg ggtagatctt cctttgatgg
     3361 atatttcttc tagtatgatt agggatttta ttaaaaaagg tcgtcagccc aattatctct
     3421 tacctaagcg ggttttagac tacattactc aagaaggttt gtatcaatga catatgaaga
     3481 ttatcttcct tatagcagga ccgaactgtt agcaaaaatt gcagagcaga tgagccctaa
     3541 acggtttaaa catgtacttg acgtggaaaa ggcagccttg tccttggcag agtgttatgg
     3601 ttgtaaccca gacaaagcag ggttagcagc tcttttacat gattatgcca aggagtgccc
     3661 tgaccaggtc tttttagact taattgacaa gtaccaatta tctccagaat tagctaaatg
     3721 gaataataat gtctggcatg gtatggttgg tatttataag attcaagaag acctaggcct
     3781 aaaagacaaa gatatcttgc gagctattga aattcacacc gttggtgctg ctgagatgac
     3841 cttgctggat aaggtcttat acgttgctga ttatattgaa gaaggtcgca tttttccttt
     3901 ggttgatgac gctagaaaaa tcgcgaagct cgatttgaat caggctgttg cctatgaaac
     3961 agtgaacact gtcgcttatt tagcctcaaa agcgcaacct attttcccac aaactctgga
     4021 tacctataat gcgttttgca gctatttaaa ggaatactaa tggataatga tatgaggaat
     4081 aaaatgaaaa aagaagaact cttaaaaatt gttgtggaag caactgatga aaagcgtgct
     4141 aaagatattt tagccttgga tttagagggg ttaactagtt tgaccgatta ttttgtgatt
     4201 gcgagtgcaa caaacagtcg tcaattagag gctattgcgg ataatattcg tgaaaaggta
     4261 aaagaggctg gtggagatgc tagccatgtt gaaggtaata gccaagcagg ctgggtcttg
     4321 cttgatttga ccgatgtggt ggtacatctt tttttagaag acgagcgtta ccactataat
     4381 cttgaaaaat tgtggcatga agcgcctgct gtggctctag atgcctattt agcttaatgt
     4441 tacaaagaga ccttgtcaat acgacagggt ttttgaggat gataatggaa cataattatg
     4501 aaaaatttgc gtcagtttat gatgctgtga tggatgattc tctatatgac ttgtggacag
     4561 atttttcact tcgacattta ccaaagtcta agggtcgaaa tcgtttgtta gagttggcct
     4621 gtggaaccgg tattcagtca gttcgttttg ctcaagcagg ttttgatgtg actggtttag
     4681 acttaagcca agacatgctg gctattgcta aaaagcgtgc gcaatctgct aaaaagaaaa
     4741 ttgattttat tcaaggtaac atgctggatt tgtcacaggt ggggcagttt gattttgtaa
     4801 cctgctattc ggattccatt tgctatatgc aagacgaagt tgatgttggc gatgttttta
     4861 aagaagttta tgatgtttta gcaaacgatg gcatctttat ttttgatgtg cattcaacct
     4921 atcaaacgga tgagtgtttc cctggttatt cctatcatga gaatgcagat gattttgcaa
     4981 tggtttggga tacctatgct gacgaggctc ctcactctgt tgttcacgaa ctcactttct
     5041 ttatccaaga agacgatgga cgtttttctc gctttgatga agtgcatgag gaacgaacgt
     5101 atgagttatt gacttatgat attttgttgg aacaagcagg gttcaagtca tttaaggtct
     5161 atgctgattt tgaagataag gagccaacta agaccagcaa gcgttggttt tttgtagcat
     5221 ataaatagaa gaaaaaagta gcctttttag ctataggatt taatgaatta tcttgaatgt
     5281 tacttatatg aaaagagttc attaggcatt aaatgctatt atcaaagctt agaagtattg
     5341 gagacattac gattgacact atgtctgaag gttgctacca actttaaaga aagcattttc
     5401 tattatacgt tgtgcttaag catttgattt aactcttatt agccctataa aagactattt
     5461 agtacaattt gaggagattc tatgactgtt accggaatta tagctgagtt taatccgttt
     5521 cacaatggac acaaatattt gctagaaaca gcagagggac taaaaatcat tgctatgtca
     5581 ggaaatttta tgcaaagagg agagcctgct cttattgata aatggattcg atcagagatg
     5641 gctcttaaaa atggtgcgga tattgttgtg gaattgccct ttttcgtttc agttcaatca
     5701 gcagactatt ttgctcaagg agctattgat attctttgtc aattaggaat tcagcaatta
     5761 gcatttggga cagaggatgt gattgattat caaaaactga ttaaggtcta tgagaaaaaa
     5821 tcagagcaaa tgactgccta tttatcgacg cttgaagata cacttagcta tccacaaaaa
     5881 aaacaaaaaa tgtgggaaat ctttgctgga gttaaatttt cagggcaaac gccaaatcat
     5941 attttggggt taagttacgc aaaggctagc gctggaaagc atatacaact atgtcctatc
     6001 aagcgtcaag gggctgccta ccattcgaaa gacaaaaatc atctgctagc ttctgcaagt
     6061 gccattagac agcacctgaa tgattgggat ttcataagtc attcagtacc aaatgcaggt
     6121 ttacttataa acaatcccca tatgtcatgg gaccattatt tttcattctt aaaatatcaa
     6181 attctgaacc attctgattt aactagtatt tttcaggtca atgatgaatt ggcaagtcgt
     6241 attaaaaaag cgataaaagt tagtcaaaat attgaccacc tagttgacac tgttgctacc
     6301 aaacgctata caaaagctcg tgtaagacgt attttaacct acattttggt taatgctaag
     6361 gaacctactt tacctaaagg aattcatatt ctagggttta cctcaaaagg acaagcacat
     6421 ttaaaaaaat taaaaaaaag caggcctctg attactagaa ttggagcaga aacttgggat
     6481 gagatgacac aaaaagcaga cagcatctat caacttgggc atcaggatat cccagaacaa
     6541 agttttggaa gaatacctat tataataaaa aatgagaggc tcaattaaac tctaattgag
//
LOCUS       DAUCWU010000081         6514 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUCWU010000081 DAUCWU010000000
VERSION     DAUCWU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.081"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..494
                     /locus_tag="VGY24_001472"
     CDS             <1..494
                     /locus_tag="VGY24_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEQ4284441.1"
                     /translation="RSKKVMKTNKKTLGKSIEERPEYINDRSEFGHWEIDLVLGKKTK
                     SEAVMLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIASITADNGSEFSLLSD
                     LKAVDVYFAHPYSSHERGTNENFNGLLREYVPKGVSLNPLTSEELDNDITAINERLRR
                     LLQ"
     gene            complement(1008..1991)
                     /locus_tag="VGY24_001473"
     CDS             complement(1008..1991)
                     /locus_tag="VGY24_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4284442.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(2022..3272)
                     /gene="dltD"
                     /locus_tag="VGY24_001474"
     CDS             complement(2022..3272)
                     /gene="dltD"
                     /locus_tag="VGY24_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ4284443.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTVQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(3265..3504)
                     /gene="dltC"
                     /locus_tag="VGY24_001475"
     CDS             complement(3265..3504)
                     /gene="dltC"
                     /locus_tag="VGY24_001475"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ4284444.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(3522..4778)
                     /gene="dltB"
                     /locus_tag="VGY24_001476"
     CDS             complement(3522..4778)
                     /gene="dltB"
                     /locus_tag="VGY24_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ4284445.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(4775..6313)
                     /gene="dltA"
                     /locus_tag="VGY24_001477"
     CDS             complement(4775..6313)
                     /gene="dltA"
                     /locus_tag="VGY24_001477"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ4284446.1"
                     /translation="MIKDMIDSIEQLAQIQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(6325..6468)
                     /locus_tag="VGY24_001478"
     CDS             complement(6325..6468)
                     /locus_tag="VGY24_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ4284447.1"
                     /translation="MIKNKSRLRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         2174 a         1302 c         1113 g         1925 t
ORIGIN      
        1 aacgttctaa gaaagtcatg aagacgaata agaagacttt aggtaaatct atcgaagaac
       61 gtccagaata tattaatgat cgttctgaat ttgggcattg ggagattgat ttggttctcg
      121 gcaagaaaac caaaagtgaa gctgttatgt tgactttggt agagcgccag acacgctatg
      181 cactaggagt taagctagaa gacaagcagt cccaaaccat taaccgtgct gtcacgcatc
      241 tcatcagtca gtatcctatt gcatccatca cagcggataa tggttctgag tttagtttac
      301 tctcagactt aaaagctgtt gacgtttact ttgcacatcc ctattcttca catgagagag
      361 gaacaaacga gaacttcaat ggcctcctca gagaatatgt ccctaaagga gtctcactta
      421 atccactaac ctctgaagaa cttgacaatg atattactgc catcaatgag cgcctaagac
      481 gacttcttca atagcaatcc tcaaaattcc tgtttgagct atcccgaaca gcttaacctc
      541 tggaactcta gttatctatg aactcgttgc acttgacttg acaacttgcg agagcatatc
      601 agtagttttt ctgctaattg aaaacagggt atttactgtc acataagtct gtcctttctt
      661 tttttctaca actttatttc gtcagcgtaa ataaaacaaa agctctataa tctcttgaat
      721 gggtaaatcc actgtagaga ttatagagct ttttgaatat caaaaaagtc ccataagatt
      781 tataatgaaa agcaaccaaa ctctcattag aaagactctt atggaacata tcaatcatac
      841 cttacaacac atcggaatta aagacaaaaa tatcactcta aacaatgttt tgaaggatga
      901 atctcctact gagctcgtta ctacttatca gaaaggtaga taggaaatcc tgatgaagag
      961 tgtgtccaag ggattatcaa ttaggtctta gattacaaaa tagtaattca ggtatgctct
     1021 actgttttca acactagttc tgcaatttgt tttcgagctt gcttagcttc tcttattgga
     1081 aaaataacat acgcatgaat cattttttct gcaataatca gctgattagc aattcgttgt
     1141 cttgttaatt gatcatgtaa agacataatg tctgggtaaa aaatctcttt tgtcccaaca
     1201 aaggtcgtta tttcaggcaa atcctcataa tcaccataca aaggactgac ataaggaagt
     1261 tgcatattac cttgaccacc tgcccatagt tcacctatat cacgaagacg acgagcatct
     1321 aggtaagctt cttctgcttc aaaatccttg taggcttgat ttttaaaact aacatctaac
     1381 caaggagata taagaattaa ttgctttggc tggggtgctg ttttagtatc tcgtatatac
     1441 ttagcaaacg ccaaagccat acccccacca gaagaatcac caatgacact aacttgactc
     1501 ggtccattag acaaacaaaa ttgctggtaa gcttcaacta atttaggata gacatcttga
     1561 taagtagcat acggtacttt aggaaagtta ggaaaaacaa ccttggcatt agtcagctgt
     1621 ataagatgct taaccgtctt aaaatgaagt ttttcgggtc gtcgtaaata gacactgcca
     1681 taaagataat aaataacagg ctgatgactt tgcttttgat cattaataat aaaaacttgc
     1741 atcccgttca catcgtattc ttgagttgaa aaacctacta tctcttcagg attatctaga
     1801 tgataaggga tattacgatt acgctgcgtc tcaaccccct tataattatc aactttttta
     1861 gataacttat actttctata agcaaacatt ttctcaacca accaacttga aaaggagcgc
     1921 ttttgaccat attcaactat caaaatacct agtatcactc caaaaaaaat caccacagca
     1981 ataatgacca cttattatcc tcgttttcta atattatttt actattgcaa gtctttccta
     2041 tttgcccaaa ttttactata aaaataaggg ttcattttat agttgggcac tggctgcttc
     2101 gtttctagaa atggttgcac tttcttatca aaagctaacc agccattcca accgagatgg
     2161 atggtatctt gcataaagta ggactcacca ccatcttttg agaagtcagc aatgcgatga
     2221 aatccttgtg actttaactg gaattttatt ttacgaactg ccgcttgata cttatcttga
     2281 tttaagccgg tataatccgc ccaagcttta ttaacaggag ttataacaaa gagtacatct
     2341 gtttttcgtt tagcaaattc tgataataaa agctgaaaat cattgtattc tggtgacgcc
     2401 aggtaactat aattaacttg gaaattctta taaagattgt atttaggtgc tattcgttta
     2461 cgataaaatg tatttttaat cccaaaacgg ttgttggttg ttgctaattg gcctctctta
     2521 gttgctaacg cattcaattg tttatacgaa aatactttag gtaatccctt aacgcgaggc
     2581 aaaattcttt tttcatagtt agtagatttg cctaaaaaac taaaaaggga ctcttctctc
     2641 aatgctactt gatgttgaca tttcaaaata gctctgtcta accgactaag agacttaccc
     2701 ttacttactt ttttcaataa gtttgattta gacacaccag ggttaagctc aagcaaacgc
     2761 tttgctgcaa actgtgattc tttatcagtt ctagctttta gtaaaaattc aatcacttga
     2821 gtgttagaca agtacatctg aaccgcacta ggattaatcc cttgaacagt aaaccattga
     2881 ggagatacta caaagatggc ttttttcttt tgcatttcat tggtaatttg ttgcatacca
     2941 taataatgcg acaaagatgc tgatcctctc ttaccaatta aaaatggtct atagctccgc
     3001 ttgtagcgct ctgcaagcac cgaaggatgc atactatcca ttcgactcca ttcgctagaa
     3061 ccaaaaaaag gcacaaaacg acaagtctca tctgataaag cttgtctttt aatcaaacca
     3121 tttttaaaag aactatctgt gatcgcaacg gcatttgctt tttcctgagc tatggaatga
     3181 tcaagttgtg tgggaaaact aaaaatagta atcactacta aaacaaaggc aataagaaga
     3241 ggacctagaa ttaaccagag tctcttaagc atgttgcaat tcctctacac cttgaacaat
     3301 cttagttact gtgttccaat catcacgacc aaattctgaa ataggtactt taatattgaa
     3361 cgttgactct aactcaacaa tcaattcgac tgtcccaaga ctatccaaca cacctgcatc
     3421 aaacaagtct tcgtccatca tctctgaaac atcttccata aacaaacggt caaacaattc
     3481 aattacagtt tcttcaatag acatttttat acttcctttt attaatgtag tggtctgtta
     3541 aaccaaaggt catttaaaaa accagagaat aacagaagtg aaaacatgac aacgtggaaa
     3601 gttaccacga tacataagac atggaatgcc ctgctttgaa agaggggtga taagcctttt
     3661 ttcttacggt gacggttgat ttcttttttc ttacgaatcc aagcatcatt aataataagt
     3721 cctataccat ggaaaagccc ataagcgata taataccagg ttaagccatg ccaaaatccc
     3781 atgactagca tattaaccag ataggcaaca cctgaagtga catttcggtt tttaaaggtt
     3841 ttatgcttaa tcaatagatg aaccaatcgc ataaacacat aatctctaaa ccagaaggac
     3901 aaactcatgt gccatctatt ccaaaaatct ttcaaacttg cagataagaa aggcatgtta
     3961 aagttttctg gtgttcgaat acccaatagg taggaaatac caatcgcaaa catcgaataa
     4021 ccagcaaagt caaaaaagag attcaagcca tacacgtaca taaccagaat agtttcctta
     4081 ttaaagtagc caccaaccat caaagcctta ttttcaagga gaggaagtaa gatgccaccc
     4141 aaacaatagg aaatgatatg cttgtataga aaacctaaca tgagatacat gactgcctta
     4201 ttgagcattg caaaataatc atcacgttca ggaagtttat gataatcttc ctgaaaacgt
     4261 ctgaagcgat caatggggcc acttgagaaa gtggggaaga aaatcatgaa gcgaataaaa
     4321 tccggtaaac gaacctcttg gagagtcccg tcacgcatct caataatcat tccaatactt
     4381 ttaaaggtca aatatgaaat ccctagaaaa ctaaagagtg tttgagaaga aggacctgtt
     4441 aggggagcaa ctttgaccca gataaggggg aataaggcca tcgcaatagc taagtaaaac
     4501 actcctgcac tattagcttt ctcacggtat ttctggtaag caaaaacact gatgatttgc
     4561 cacaaaagat aaaagagaaa agcaactaat tgttgataat gatctcctcc aaacataaag
     4621 agaataaata ccaaactcac caaggtttca taagtgctta agcgcttttg tttaaaaatc
     4681 ccaatgatga ctggtaagaa agccaaaata agatagacaa agtaaatggg gttaccgtaa
     4741 ggttccatgt agggaatgtg gctaaaaaac atcatcatcg gttattgacc tcattaatca
     4801 aagttttaat atctatctta ccatttggag ttaatggtag actatctcga taaagaaact
     4861 tagaaggcat catataactc atcatatgat ccttaacaga ggctttgata gctttggtta
     4921 actccaattc cctatcaaaa cgctctttaa ccccatcttt gacaacgata taggccaaga
     4981 gattttgaac cttatgttct ttattgtacc ttggaacagc cacagccgat gctaccattg
     5041 gtgattgatt caactgttgg gaaacatctt ctagttcaat acgataacca gcatatttaa
     5101 tctgaaaatc caaacgccca ccatacagca aaatattatc ttctgtcagt gagccaatgt
     5161 cccctgtatg ataagcaggc tgtcctttaa aagtaaagaa agcttctgcg gttttctcag
     5221 gattgttgag gtaacctttt gacacagcag gccctgtaac aataatttca ccctgctccc
     5281 ctgacgataa ctccttacca tcttcatcaa taatataggt cggagaatct ggtttggggt
     5341 agccaatcgg caagcgggta taattatcca ccatttctct ggtaatttca atagcggata
     5401 aggctacagt cgcttctgtt ggtccatagg cattgataat cttggcactc gggaaacgtt
     5461 caaagagttt tcgagccgta gaaacggtta attcttcgcc atcaaaataa aaatgcgtta
     5521 aagctggcat cttagcctgg caaaaatcat cgctaagcat ggccatatca gcaaatgaag
     5581 gagtcgatgt ccaaatgcca actggaagtt gcgcaatcgt tgtaaataac tgtttaaagt
     5641 ctgccaccaa ttcttttgga agtgcaaata aggttccacc taaagccaag gtaggtgccc
     5701 agtacatgac agaaaggtca aaggaatagg gtggctgcgc cagcatttgt ggctgtttag
     5761 gtacatcaaa tgctgcatct tcgatcatcc agttggtaaa gctcaaaaga ttgtcatgtg
     5821 aaatttgcac cccttttggt tggcccgttg ttccagaggt gaaaataatg taataattat
     5881 catctccttt aacagaatgt gttcgctcat agggcatttc tgctaatttt gctgactcaa
     5941 tctctgatag tgagacaagg gaaatccctt caatagtaag agggaattcc tcaatagcaa
     6001 tgattaaact gggctttgca atctcaataa tcgctaaaat acgttctggt gctgaatgca
     6061 catctactgg gatataggca tgaccagatt tggttaaagc tacaaaagta gctaacatat
     6121 cataagtttg cgccccaaaa accaaaactg gagattttgc cagtaaagct aagctatcta
     6181 taaatgcagc aatgctatca gaatctcttt tgagttgtcc gtaagtgcgg cgttccccta
     6241 aacaatcata aactggaaaa tcagcctgta tctgagcaag ctgctcaata ctgtcaatca
     6301 tatccttaat catcagtcaa tctcctaaaa ttcattataa ataaagttac tttgaccatg
     6361 tcctagataa ccaaagaaat agaccagcaa cattaaaatc agataaaata aaattgtctt
     6421 gccaataaaa acagcaattc ctctcaatct tgatttgttt tttatcatac tttaccttct
     6481 tgtattagtg aaataatgtc ctaaaaaatt ctac
//
LOCUS       DAUCWU010000082         6494 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUCWU010000082 DAUCWU010000000
VERSION     DAUCWU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.082"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..88
                     /locus_tag="VGY24_001479"
     CDS             <1..88
                     /locus_tag="VGY24_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ4284448.1"
                     /translation="YIFYYNNKRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(137..868)
                     /locus_tag="VGY24_001480"
     CDS             complement(137..868)
                     /locus_tag="VGY24_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ4284449.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQKTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            983..1348
                     /locus_tag="VGY24_001481"
     CDS             983..1348
                     /locus_tag="VGY24_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ4284450.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVREQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            complement(1468..2688)
                     /locus_tag="VGY24_001482"
     CDS             complement(1468..2688)
                     /locus_tag="VGY24_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4284451.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPVKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISIGAMW
                     TIGQLKAERYQQLR"
     gene            3062..4546
                     /locus_tag="VGY24_001483"
     CDS             3062..4546
                     /locus_tag="VGY24_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ4284452.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            complement(4651..5052)
                     /locus_tag="VGY24_001484"
     CDS             complement(4651..5052)
                     /locus_tag="VGY24_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ4284453.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(5137..5915)
                     /locus_tag="VGY24_001485"
                     /pseudo
     CDS             complement(5137..5915)
                     /locus_tag="VGY24_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            complement(6080..>6494)
                     /locus_tag="VGY24_001486"
     CDS             complement(6080..>6494)
                     /locus_tag="VGY24_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ4284454.1"
                     /translation="AKKYKKLTKQKEELLDEKKTLELTIKDENSIKERLKQFKKVLEN
                     KEIIEEFNRTVFESIVDKVVVGRIDKDGTVHPYDLTFYFKTGDKDSQDSNNFKDKRKN
                     AKDNDINKLCSYKDDEDKKLCSQSKDNTRGVCKFT"
BASE COUNT         2003 a         1420 c         1166 g         1905 t
ORIGIN      
        1 ttacattttt tactataaca acaaacgaat caaagcaaaa ctaaaaggat ttagtcctgt
       61 ccaatacaga actaaatcct ttcaataatt atttgtccaa ctttttgggg tcagtactga
      121 caggggaggt ttctttttat agtgagctct gacaataagg tgccaggctt tgcaattcct
      181 caggcgtcag gcgcctaaac tccccttctg caaggctagg gtctagtttt aatggtccca
      241 tgctgagacg ctgcaagtca agcactccct tcccacaggc tgctaccatg cgtttcacct
      301 gatggaattt tccttcttgt atggtaattt taaccaaact tgttttctgc tgtaaatcac
      361 tagcaagcac ttctaaatga gcaggcaagc actggtgatc ctttaaggaa atacctctag
      421 caaaataatc tttatcagct tcagtcatga tgcctgctac tttagccaag tactctttcg
      481 taacatgttt tttaggggat aaaaggtcat gggctagttg tccattattg gtcaagagca
      541 aaagtccacg ggtatcctta tctaacctac ccacaggaaa aactgctttt tgcctagccg
      601 tgtcatctaa caactccata acagtagcct gctgtctgtc ctgggttgct gacacatacc
      661 ctgacggctt gtttaacagg taataaacaa aggattcata gaccaagggc gtgccttgat
      721 acgtcaccaa atccttgtat tcatcaatgt gtgctttggc agaggtttca accttttgat
      781 tgacgaaaat agccttcttt tttaagagaa gtttgacttg gctcctagtt ccaacaccag
      841 ttgccactaa aaatttatct aagcgcatca taaccctttc aaagaaaatt cattattaag
      901 tataacataa aaaaacagtg gtttcatgac tgtaagggct taatatatta taataggctt
      961 atagaaacga ggagattaac ttatgactat ttattctcac acttatgaaa cccgtcccaa
     1021 tcattcggct aaagccatgg gctcaggaac gctgaatgtc ctagctactc ctgctcttgt
     1081 tgcttttatg gaaaatgctt cttacttatt tgcccaagaa cagctcgaca gtgactttac
     1141 aacggttggc agcgagatag ctatccaaca tttggcagct tcagccattg gagatcctgt
     1201 tacaatcatc attacagccc ttaaagaaga aggccgaaaa tacgatttcc gcttggaagc
     1261 ttttgtgaga gaacaactca ttggaaaagc ctgtcatact agagttaggg tgaacagtaa
     1321 acattttatg gccaaagtag cggagtaaaa caaaagaaaa gaaccaaaaa ggtaagcaat
     1381 ccctaagtgc ttaccttttt gcttatctca atcaataaaa tagctttgtg agtgaaagta
     1441 actcaaatac taacacccat ctctttgcta acgcaattgt tggtagcgtt ctgcttttaa
     1501 ctgaccaata gtccacatgg ctccaatact aatcacatac agagaagcta ggaaaatcat
     1561 gaccattgaa atacctgagg catctaaaat actaccaata gcaacggacg aaaatcctcc
     1621 tagagcacgg ccaacattca aaataacatt attagccgtg gatcgaatgt gatggggata
     1681 aagtctggta atcatagccc cgtagccagc aaacatccca ttgacaaaaa aaccgacaat
     1741 agcacctcca ataaccatac tcgccatgga attggcaaat tgaaaaaggt aaacacaaat
     1801 agatgatgct aataaaaaga gggaataaat cagacgtgga ccaaagcaat ctaaaagctg
     1861 accaaaacac agcatcccca aacacatgcc gacaatagta gccaccatcc acaaggacga
     1921 actttttact gaaaggttta agcttgtttg aataatggtt ggcaaccaat tcatcattcc
     1981 aaaatagccc gcaatctgca cagtcgtcat gaccattaaa gcaagggttt gagctgttaa
     2041 ggattttgtt ttgaatagtt cattgatttt aactggttga ctgcattctt cctcctcttg
     2101 cccataatga tcccaaattt tttggtcatc tatggctaaa gtcatccaaa tcaccaagag
     2161 aatcggaagt aatccaaaga gaaaaaggcc acgccagcct agacttggag ccagccaccc
     2221 tgctaaaaga gccgaactaa tggagccaag ctggccagca ataccattta aagaagacat
     2281 ccgacccatt ttttctggtg gtacaatccc tgccattatg gcaatggcta caccgtactc
     2341 acctccaact cctatgccag caataaatcg catcaggtac aggtagggca aagactgggt
     2401 aaaataaatt aatcctgttg ctactgaaaa cagtaaaatc gtccatttaa agaccttaaa
     2461 cttgtggtgc ctatcagcca gcaacccgaa taagaggcct cccaccaaca ttcctaaatt
     2521 ggtaatggtc gcaatccagc caccctgagt gccactaaga cccaagtctg tcataatgga
     2581 agacatggaa aaggcaagaa acatgacatt caagtcatcc gttcctgaag ctgcaatagc
     2641 tgccactaaa gctcgcttat ttgtggtgtc caatgataat gttgacataa ttgtctcctt
     2701 cttaatagta gctcactgac taccttaatt aattgaatac cctttgtcca aaggtcaaaa
     2761 aaagacttgg aaacaagtcc gcaagtccat caaagttcac taaataataa gagatgacaa
     2821 aaaaactctc tttactgtta agactttcat gcttcacgga acatgtttaa agtagtattt
     2881 aattattact agtatagtaa agctcatttt aattgtcaag ttcttttgaa catagcggat
     2941 acatttttct tctaagaacc aagcctaagt ttttttaaaa agtcacaaaa tgaggtatcc
     3001 ctgcaaaaaa ccttttcttt cttggaattt ctttgttata atgctttaca gaggtgaaaa
     3061 aatgaagact attgcttttg attctaacaa gtatttaaac ttgcaaagag atcatatttt
     3121 agaacgtatc agccaatttg atggcaaact ttacatggaa tttggtggta aaatgttaga
     3181 agattaccat gctgcccgtg tacttcctgg ctatgaacct gacaataaga ttaaattact
     3241 caaagagctc aaagaacaag tcgaaattgt aattgccatt aacgctaata atattgaaca
     3301 ttctaaagcg cgtggtgatt taggcatttc ttatgaccaa gaagtctttc gcttaattga
     3361 caaatttaac acgttagata tttacgtagg ttctgtcgtt attacccaat acaataacca
     3421 acccgctgca gatgcctttc gcaagcaact cgaaaaaaat ggcattgctt cttacctgca
     3481 ttatcctatc aagggctacc caacagatat taatcatatt atctcatctg aggggatggg
     3541 gaaaaatgat tacatcaaaa cgagtcgtaa ccttatcgtt gttacagcac caggccctgg
     3601 gtctggaaaa ttagccactt gcatgtccca aatgtatcac gaccaaatca atggggttaa
     3661 gtctggctat gctaaatttg aaaccttccc agtttggaat cttcctcttc atcaccctgt
     3721 caatctggct tatgaggcag ccacggcaga cctagatgat gtgaatatga ttgacccctt
     3781 ccatttggaa acttatggca aaacggccgt taattacaac cgtgatatcg aggtctttcc
     3841 tgtcttaaat cggaccttcg agcgtatttt gagcaagtct ccttatgcct caccaaccga
     3901 tatgggtgtt aacatggttg gtttttctat cgtcaatgaa gaagctgcta tcgaagcttc
     3961 taaacaagaa atcatccgtc gttattacca aaccctcgtg gattttaagg cagagcgggt
     4021 cacagaatct gctgtcaaaa aaattgagct cttgatgaat gatatcggtg tcacccctga
     4081 cgatcgtcac gttactgtag ctgctcatca aaaagcagag caaacaggcc aacctgccct
     4141 tgctctccaa ctaccaaatg ggcaaattgt gacaggtaaa acatctgaac tctttggacc
     4201 aactgcagct gttattatca atgcgattaa aaccttggca aagattgata aaaccactca
     4261 tttgattgaa cccgaatacg tgaaacctat tcaagggctc aaagtgaacc acttaggcag
     4321 tcacaatccg cggcttcatt ccaatgaaat tctgattgcg ttagccatta ctgctatgac
     4381 tagtgaagaa gctaatctag ccatgaaaga actcggcaat ttgaaaggta gcgaagccca
     4441 ttctaccgtt atcttaaccg aagaagataa aaatgtactc cgaaaacttg gggtaaatat
     4501 tacctttgac cctgtttacc aacatcacaa actttaccgt aaataaacca taaccacccg
     4561 tctaacgggt gatttatggt atctctcaat aaaggaaaaa agccccaatt ctcagagagt
     4621 tgggactttt ttcctttatt gattcattaa tcacccgtta tgacgtcggc cagtcacaaa
     4681 catggtcaag cttgtttttg ttagaagagc tcctgtttgg ttatgaatcg tcacatcaac
     4741 cacctgcgtg gttcgacctc catgaaccaa tcgaccttca accatcagct tgtctccctt
     4801 gtgccctgct tttaaatagt tagcattggc ctgtaaggtg acagattcta cgccagtagt
     4861 tctagcaacc aaaccaccta cttgatcaca tagggtgaaa agataaccac catgggcatt
     4921 cccataataa ttcaaagccg tctcagtcac ttcagtactt aaaataaggt gtcctttttc
     4981 agcaagttca atttggtaat tatcaaaaat actaataaca tttaaggtca tctcttgtgt
     5041 taaatctgac attgtttttc tcttttctaa gcttagtcta aaagcaatac aagcctcatt
     5101 atactacaaa taggaacaga aggcagagct catgtttcaa aaatcagtag cctttccatc
     5161 cttttctagc gtattgagca cttccaagca taaatggagc gcaaaactaa aggaatcagc
     5221 gccccagttg cgttgatcgt aaacctctac atctgctaga gtatctgctg taaataagag
     5281 ttgcccccaa aggatgcctc ttaattgagc tactgctgct aaggcagaac attccatctc
     5341 aacaaccgaa cacccttctc cctggcgata agccactttt tctttggttt ctcggtaaaa
     5401 accatctgtt gaccaggtga tgacctcttg ataagctaac ccctgtgcta aaagcgtttt
     5461 ctcaatcagt ctaagcatct taggatcaat atcaatataa cgagatggtg ccacgtaatg
     5521 ataacttgtt ccttcatctc gcaaagcctt aactggcact aaaaaacggt tttctgcgat
     5581 aggaaccaaa acgccacagg ttcctgtact gatcatttct ttaaccccat aaccaatcag
     5641 ccagtccatg aattgcgccg caggagctga gccaacggga gcttgagcta ggcaaatgtc
     5701 ttgaccttta taagatatct gataaacagg gtaaaccttt gtggctgaaa tgaattctgc
     5761 tactttttta ccttgatggt ctaaagcaaa agcttcaatc tcgtcttcta agaaagcata
     5821 gacacactta gctggaagct aaatagctag ataatgacgg ctgatggatt actatcacat
     5881 tctaaaaggg gaatctcgtg tttggtaata ggcattcgtt tctccttatt tttgtcatgt
     5941 ggaggcagct accttactcc ttgattagca acactacgca ctcgacgtga tgggtctgcg
     6001 ggagtcaaaa ggtccagtgg accttttgag cgtctcacct tgaaataaca gagtgaggcg
     6061 gactctttaa ctccgtttct caagtaaact tacacactcc acgtgtgttg tcttttgatt
     6121 gggaacataa ttttttatcc tcgtcatcct tgtaggaaca caatttatta atgtcattgt
     6181 ctttggcatt ttttctttta tctttaaagt tattagaatc ttgactatct ttatctcctg
     6241 ttttgaaata gaatgttaag tcatatggat gaactgttcc gtctttgtca attctaccta
     6301 ccacgacttt atcaactatg ctttcaaata ctgttctatt aaattcctct ataatttctt
     6361 tgttttctaa gaccttttta aattgcttta gtctttcttt aatagagttt tcatctttga
     6421 tagttagctc tagggttttc ttttcatcaa gtaattcttc tttttgtttt gtaagttttt
     6481 tatacttttt agca
//
LOCUS       DAUCWU010000083         6378 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUCWU010000083 DAUCWU010000000
VERSION     DAUCWU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.083"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            59..352
                     /locus_tag="VGY24_001487"
     CDS             59..352
                     /locus_tag="VGY24_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ4284455.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            349..666
                     /locus_tag="VGY24_001488"
     CDS             349..666
                     /locus_tag="VGY24_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ4284456.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            684..1310
                     /locus_tag="VGY24_001489"
     CDS             684..1310
                     /locus_tag="VGY24_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ4284457.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPIVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            1462..1857
                     /locus_tag="VGY24_001490"
     CDS             1462..1857
                     /locus_tag="VGY24_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ4284458.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISIVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(2117..2758)
                     /locus_tag="VGY24_001491"
     CDS             complement(2117..2758)
                     /locus_tag="VGY24_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ4284459.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(2778..3755)
                     /gene="dusB"
                     /locus_tag="VGY24_001492"
     CDS             complement(2778..3755)
                     /gene="dusB"
                     /locus_tag="VGY24_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ4284460.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(3742..4614)
                     /gene="hslO"
                     /locus_tag="VGY24_001493"
     CDS             complement(3742..4614)
                     /gene="hslO"
                     /locus_tag="VGY24_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ4284461.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIINDKA"
     gene            complement(4761..6254)
                     /gene="rofA"
                     /locus_tag="VGY24_001494"
     CDS             complement(4761..6254)
                     /gene="rofA"
                     /locus_tag="VGY24_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ4284462.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYIEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
BASE COUNT         1989 a         1114 c         1356 g         1919 t
ORIGIN      
        1 agtagaaatc tttagttttc ttatttcaga tcgtccaatt tgatcttttt atgataaaat
       61 gagggatatg agtaaaaaga aaataggtat gatttcaggt atctttggat ttagtttagc
      121 tattggccta ggaatagtta tcaaagatta ttgccaagac aggcagcgcc gacaaatgac
      181 aagggattta cgcacctttt tttcaccttt aggacaaatc gaagttttat atatcaatcc
      241 ttgtcaggtc aaacaagatt atatttcagg tggggttgtt atgtcaaacg gcaaacagta
      301 ccaattcacc tatcacagtc gacaaattag ttttgaggag gacaagggat gatcagaccg
      361 acttcttatg agtcattagc ggctcttatt gaaaaagaag ataagctggt gttatttttt
      421 acggcagact ggtgtccaga ctgtcaattt atttatccta ttatgccaga aattgaagca
      481 gaacttacag atatgacttt tgtttgtgtt aatcgtgatc agtttataga ggtagcacaa
      541 aagtggaata tttttggtat tcctagtttt gtcgtgattg aaaaaggtca ggaagtaggg
      601 cgtctggtta acaaaatgag aaagacaaaa actgagatta tgcatttttt agctgcttat
      661 caataagaaa agaggaagaa taaatgattt ttgcatacaa taaagaacaa gttggcgatg
      721 tcttgatggt tatcttacaa gacaccaaag atatcaaacg tcaagtagaa cgaaaaggca
      781 aagtagcccg tgtttttgca gaagaaagcg gcaaaaccct tgcttggaat atctttgaag
      841 cgtcaagctt gattactatt gaaggcaatg gacagatttt tttgacagac gaggaccttg
      901 caagattaaa tgcagagctt gctaaggaag gattttcaga aaggcttgaa ccgattgtgg
      961 gacctgtttt tgtggttggt caaattgttg agatggtggc tcatccagat agcgaccatc
     1021 ttaatatctg ccaagtggct attggtgaag atcaaacggt tcaaatcgta gcgggcgcac
     1081 caaatgctgc gcttggttta aaaacgattg ttgccttacc aggtgctata atgccaaatg
     1141 gtagtctgat tttcccagga aaattgcgtg gtgaagaaag ttatggtatg atgtgctctc
     1201 ctcgtgagtt agccttgcca aatgcaccgc aaaaacgtgg cattattgaa cttgatgagt
     1261 cagctgtggt aggagaagct tttgacccag ccaaacattg gaaaggttag aaaaaccagc
     1321 tgaaataaga ctgtatttaa ctaaatgacc agaaaaaaag gaataggagc ccttaatcct
     1381 tttttcttat gttttcaaaa acgtttaaaa tgaaacttat tttaccacaa tttttacgaa
     1441 taataagata aggaggtcag tatgtataat aaagtgatag caatcggtcg tttggtagct
     1501 aaaccagaat tggtaaaaac agctacggat aagcatgtag cacgtctctc tttagctgtt
     1561 aatcgaagat ttaaaaatgc ttctggagag cgagaagcgg attttatttc aattgttgtt
     1621 tggggaaagt tagcagaaac tctggtttct tatgctagca aaggtagttt gatgtctatt
     1681 gatggcgaac ttaggacccg caagtatgat aaagatgggc aagtgcatta tgtgacagaa
     1741 gttctctgcc aatcatttca actgcttgaa agtcgtgctc aacgcgctat gagagaaaat
     1801 aatgttacta atgatctagt tgatttagtc ttagaagaag atactcttcc cttttgatag
     1861 aggataatgc ttctcatcag ggttcagctg attactgaaa tatatgatga ggtgatatcc
     1921 gagcaacaaa agtgtgacta gatacttctg ataaaaataa aaaaacacct tacgagtatg
     1981 aacaaagttt caagtgctag atgttggaac tttgttcatc ataggaatag tagggtgttt
     2041 tttagtgtat tacttctttt tgttgctagt gttaaggttt acttgcttac aagcggttat
     2101 caggccacta gaactcttat aataaatgga gctctttgag tttggtgtca atcattttta
     2161 agacagtcgc caaatcttgt gggttttgaa cgaaatcaag ggaattgcca tcaattttca
     2221 tttttggaga gaccttgtag tcttcgtacc aggttgggta ttcaccgtgg acttgatggt
     2281 aatattgctc aagactagga ttgccgtcga cctgctcaaa acttcggcca cgtttttcaa
     2341 tgcgctcaag cattttgtca aaagacacat caatgtagat gagaagatca gggcgttttt
     2401 taggcatgcc ttctagttct tctaacatgt tggcgagaag ttcctgatag atgtcaagtt
     2461 ctgtcttagt gacattgcca tttttgtagt taagttttaa gaagagttcg tcttcaaaaa
     2521 tagagcggtc aagaatgttg ttatcggctt gataagcttc tttgatggac ttaaaccgtt
     2581 tattcaaaaa ataaatttga agtaaaaagg cgtatttttt agggtcttgg tagtagaggt
     2641 ctagcacggg gttattatca acagcttcgt aaaaaacgtc agttcctaaa tgctctccaa
     2701 gtgcagcagc aagcgagctc ttgccagccc caattgttcc agctagtaca atcaacatgc
     2761 gtctccttct aatgtcatta gcgtactgtt tcgaagatgg cttcaacctc agctagagtc
     2821 tcagcgcgtg aaacagcacc acgaactttg gcagcacctg ctgttccgcg aagatagtgt
     2881 ggtgctaagc cccgaaattc ccgaacagca atggtttcac ctttgaggtt aatcaggcgt
     2941 ttaaggtggt ctttggcgat gtctaatttt ttggcaaagg gaagatctgg caattcttga
     3001 ccagtttcaa agaaatgatt gatttgggta aaaagataag gattgttcat ggctgcgcgg
     3061 ccaatcatca cggcatctac accgatttct tcaatcataa atttggcgtc ttgcaccgaa
     3121 cgcacgtccc catttccaat aaagggaatc ttagtgatag ccttagaaac tcgggctaat
     3181 gtctcatggt cgcaggttcc tgtatacatt tgttcacggg tacgaccgtg catggcaaga
     3241 gcagatacac ctgccgactc agcggcaagg gcattctcaa cggcaagtga actatccgcc
     3301 caacctgtac gcattttgac agtcagtgga atatctaaaa cagacgtgac ttccttgaca
     3361 atgtggtaaa tcttatcagg gtcacggagc cacttggcac cagcctcgtt tttgacaact
     3421 ttattaacag ggcagcccat attaatatca acaatatcgg ccttggtatt ggtttgaatg
     3481 aaatcagctg cacgtttcaa cccttctgca tcaccaccaa ataattgaat ggacataggg
     3541 tgctcattct cgtcaatatg gagcatatgg agggtctttt cgttgttgta gagcagccct
     3601 ttttcggaaa tcatttccat aaccaccaaa ccagcaccaa attctttggc gatggtacga
     3661 aaggcagagt tggtcacgcc agccatggga gcaaggacag tgcggtgagg gatttccaca
     3721 tcaccaatcc taaaggaaga attaagcttt atcattgata atggcctcca aatcactttc
     3781 attaaattgg tacttggtac cacaaaattg gcaaacaatc tcagcaccct tatcttcgtc
     3841 aatcatggcc tgtaaatctg cttttggcaa agtcattagg gctgcttcaa aacgttctct
     3901 agaacagtca cactgaaaac tcagaggttc ttcagaaaga cgcttgtaag gttcatctcc
     3961 atagatggcc tccaaaaggg catccacatg atttttggaa gctagtaggt gagaaatggc
     4021 aggcatttct tgcaggcgtt tttcatagcg agcaatctct tcttcagaag ctcctggtag
     4081 cacctgcacc ataaagccac cagcaacctt gaccttatca ttttcatcta aaagcacatt
     4141 aagtccgatt gcagatggtg tttgctctga ttcggttaag tagtaggcaa aatcctcacc
     4201 aatttcccca gtaataagag gcgttgttga ggtgtaaggg ttacctgtgc cataatcaat
     4261 aatcgtcaca aaatgaccat tccccataaa aggcccaaca aggacttcac ctgttgcggt
     4321 ctttttaata tcgacaccag tattttgaat gtaacctttc acatgtcctt tggtatctgc
     4381 cacagagata atgtgtccaa aagaagaatc accaatcact ttgacggtga ttttactatc
     4441 gcctttttga ttagccgcca aaatctggtt ggcgatcaag gtacggccaa gcgcaacagt
     4501 agacgatgac agcgtgttgt gtttttcttg agctaatgca accgtctcag tgctatcaag
     4561 gacataagcc ctaaaagccc ctgattgtgc tattgattta attattttat ccataactta
     4621 gattatagca taaagtgtaa aacttgtatt gacaggattg cttgaaaaat caatacagat
     4681 agacagtaaa aaatcccaga gaatgactcg ggattaagtt aaggtgtggt ctaaagtcgc
     4741 gcaatgtggt gacatggtgc ttatgttaat tgcttggtta aatcagcttg gaatttttcc
     4801 tctttaactt gatacatcaa ttcttggata gacagaatcg attcatcaaa agatatttca
     4861 gcaacagcaa ttccttttgt aagttcatgg tgaacaaaag gaatcagttg actgtgagtg
     4921 atgaccaaat ctggctttaa atcaggaatt tgataaacat tatcttgcaa tagataatag
     4981 gaatgaaaat caatgctttt atccgagaaa taccttggaa aagaatccgt taggagatga
     5041 gcattgataa aattactggc tacgaaaaca acaactaaag gaggttggat atttcttaga
     5101 ctttgctcga tatagtgaca aaaaagatga aaatgcttat ggttcaagtc acgcttacca
     5161 ggaagtttgg ccatccactc ttcgacaatt aactttaagg acgtatagag cttttggttt
     5221 cctttatagt acggagaaac gaataagttg gtctcaggaa taaaatgttg cagattaaac
     5281 aagaatgatt ttgaaaaaaa cataagagct tttactaaac tagccttttg ctcttttagg
     5341 ttaggtaaaa gagtgatgat aggatttaaa agcaggcgaa aagtatcatt ttcttcaaaa
     5401 agttgacaac attgtctgat atgctcaggt gtccattgta agctcgcaaa agaattatta
     5461 gcggtgatat aaattaaata gaggtagtcc aaatctcctg ctgaaaagtt tagttggcag
     5521 taagtttcga taatatcatg gctacttttt ttcaaagaat catagacaaa aagttttttt
     5581 aattgttgaa aaattctggt ttggggaata gttaccgaaa attgatgacg cttccacgat
     5641 aaagccaata aaatgtcata gaaagaaaac gattccgata accaaggaga ggttttaagg
     5701 tgggtggaac tatgggataa aaagctatga atagtgtttt tgtcttgctg cgtcaagtca
     5761 taaactttaa tgccaaactt actatatagc agagcgatga ggtaacggat gcgatattcc
     5821 tcaccgacaa tcttgttctt agagagtttt aattcaaagt ttcttaataa aggaatcaat
     5881 gcttcgcgca tccgataagc tgaggagttt gataaaaaat gacttcttgc aaaatccgta
     5941 aggggacgag agtgggaacc attttttatt aaaaacgcta gtaattgtaa gacattagat
     6001 gatgcataga gttggtaaag ataagtttct ttaaaaggat gtgtaaattg gcaagatatc
     6061 atcctttttt ggatggtcat agacagacta ccagggaaaa aggcattcag ttcctcacaa
     6121 taatggttta gttgtaaaaa ggttaagcca gttttttctg ctacctcagt tattggcaaa
     6181 taagatgtct taaaaaacaa gataattaac tgacattttg attcgattga tgattccaag
     6241 tatttttcta tcaaggaaag ggacctctct tcctaaactt agaaagccaa ataagtcttt
     6301 gataagtgta ttctcctatt tcaattctat ctaagctaaa aatagaaatc aaatctaatg
     6361 gtttttggtg aaaagtcg
//
LOCUS       DAUCWU010000084         6254 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUCWU010000084 DAUCWU010000000
VERSION     DAUCWU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.084"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            69..995
                     /locus_tag="VGY24_001495"
     CDS             69..995
                     /locus_tag="VGY24_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ4284463.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            1049..1134
                     /locus_tag="VGY24_001496"
     tRNA            1049..1134
                     /locus_tag="VGY24_001496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Leu,seq:aag)
     gene            1340..2221
                     /locus_tag="VGY24_001497"
     CDS             1340..2221
                     /locus_tag="VGY24_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ4284464.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            2603..2791
                     /locus_tag="VGY24_001498"
     CDS             2603..2791
                     /locus_tag="VGY24_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ4284465.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            2943..3308
                     /locus_tag="VGY24_001499"
     CDS             2943..3308
                     /locus_tag="VGY24_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ4284466.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            3308..4972
                     /locus_tag="VGY24_001500"
     CDS             3308..4972
                     /locus_tag="VGY24_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ4284467.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKTKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            5209..6099
                     /locus_tag="VGY24_001501"
     CDS             5209..6099
                     /locus_tag="VGY24_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ4284468.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
BASE COUNT         2017 a         1081 c         1360 g         1796 t
ORIGIN      
        1 aaaagaaaaa cgtgatattt ttaggttagc actttctttt tcagtcattt ttttgtaaaa
       61 tacttggtat gaaaactatt cgaatagcaa agtatttagg aattctattc cttttgataa
      121 ctcttattag cgtaggtgct agtttttatt tctttcatgt tgcacaaata agagaagaga
      181 aatcgtttat taataataag aaacggagta caaataatcc attataccca gctgaacagt
      241 cttttgacgc tttaccttac gaaaaacgtc aactaacaaa tcgtgggtta aaacaagtgg
      301 ggtggtactt accagctgct caaaaaacaa aaaagacagc tattgttgtt catggtttta
      361 cgaatgacaa agaagatatg aagccatatg ccatgctttt tcatgatttg ggctataatg
      421 tcttaatgcc agacaatgag gcccatgggg aaagtgaagg gaacttgatt ggttatggct
      481 ggaatgaccg ccttaatgtc atggcttgga cagaccaact gattaaggaa aaccctgaaa
      541 gccaaatcac actctttggc ttatctatgg gtgctgcaac agtaatgatg gcaagtggtg
      601 agcgattgcc tgcgcaagtc acctccctca tcgaagattg cggttatacc agtgtttggg
      661 acgaattgaa gtttcaggcc aaggctatgt acaacttgcc tgcctttcct ttactctatg
      721 aagtctctgc cttatctaag attcgagcag gttttagtta cggagaagcg agctcagtga
      781 aacagctggc taaaaataaa cgtccaactt tatttatcca cggtgataag gatgattttg
      841 ttcctacaaa aatggtttat gacaattata aggccacgaa aggccctaag gaaatcttga
      901 ttgttaaagg ggcaaaacac gccaaatcct ttgaaacaaa cccagaacaa taccagaaaa
      961 aaattgccgc ttttttgaaa aaagttgaga aataagtatt gacagttaaa ctcacagttg
     1021 ctataataat ttatgttact gatgagtagc ggggatggcg gaattggcag acgcgcagga
     1081 ctaaggatcc tgtgaccgct ttaggtcgtg tgggttcaag tcccactctc cgcataatgt
     1141 aagaggtgaa tcattgattc gtctcttttt ttggttaaat gaaggataag aaggcataga
     1201 tttactggag aaaacttttg ataatctgct tttggaaaac gctttaagct tgtgaaagac
     1261 tagaaattta tggaaaattg tgctagaatg aacaatgtaa acggggagtt atcccgaaaa
     1321 ataagaggag gccttacaaa tggcaatcgt ttcagcagaa aaatttgtcc aagcagctcg
     1381 tgaaaacgga tatgctgttg gtggatttaa cacaaacaac ttagagtgga ctcaagcaat
     1441 cttgcgtgca gcagaagcta aacaagctcc agttcttatc caaacatcaa tgggtgcagc
     1501 aaaatacatg ggtggttata aagtatgtca atctcttatt actaaccttg ttgagtcaat
     1561 gggtatcact gttcctgttg ctatccacct tgatcatggt cactacgaag atgctcttga
     1621 gtgtatcgaa gtaggttaca cttcaatcat gtttgacggt tcacaccttc cagttgaaga
     1681 aaaccttgct aaaactgcag aagttgttaa aattgcacat gctaaaggcg tttcagttga
     1741 agctgaagtt ggtacaatcg gtggtgaaga agacggtatc atcggtaaag gtgagctcgc
     1801 tccaatcgaa gatgctaaag caatggttga aactggaatt gacttcttgg cagctggtat
     1861 cggtaacatc cacggtccat acccagaaaa ctgggaaggt cttgctcttg accacttaga
     1921 aaaattaaca gcagctgtac caggtttccc aatcgtattg cacggtggtt ctggtattcc
     1981 tgacgatcaa atcaaagaag caatccgtct tggtgttgct aaagttaacg ttaacaccga
     2041 aagtcaaatc gctttctcta acgcaactcg tgaatttgct cgtaactacg aagctaacga
     2101 agcagaatat gatggtaaaa aattatttga cccacgtaaa ttcttggctc caggtatgaa
     2161 agctgttcaa ggtgctgttg aagaacgtat cgatgttttc ggatcagcta acaaagcata
     2221 atttagagta ttaaataccc taaagccctc ttgagggctt ttttagatac aattgacatc
     2281 ttaaaatgga taccagtttc tgtgaaacaa tctttgattg tgaacaaaca aagcataatt
     2341 tagagtatta aaatctggac cctgctatct atggtaagtc tttttgttat tgttaaagag
     2401 gttaagcgga atgttaatat gttttagcta aaaaatgtag tgaaaaatga gtacgtagac
     2461 tattgtaata gtaattccgt aaaattgttg aaaaagaaaa tgggaaatac cttgtcaaat
     2521 taagcaccct atgatagaat agataagtat gtggtgctag cacatccgta aacatattct
     2581 acgtaggagg aaacagatca aaatggctaa agtatgttat tttacaggac gtaaaaccgt
     2641 ttctggaaac aaccgttcac acgcgatgaa ccaaacgaaa cgtacagtta aaccaaacct
     2701 tcaaaaagtt actatccttg ttgatggtaa acctaaaaaa gtttgggctt ctgctcgtgc
     2761 gcttaaatct ggtaaagtag aacgcatcta atagaaataa gccttagggc tttttttatt
     2821 agcttatttc tatccataag ctagtcaggg taataaacgc tattgttaac tattctatct
     2881 cttttacatt acttctaaaa tatgataaaa taggatgata aaaacttttt gaggtagtaa
     2941 ctatgactgt aaaaattaat acaaaagatg gtctaatcga gctatccgac gatgtgattg
     3001 caactgtcgt gggaggatca gcaacggaaa tttttggtgt tgttggaatg gccagcaaaa
     3061 gtgcaataaa agacaatttt caatcactac ttcgcaaaga aaactacgct aagggtgttg
     3121 tagtaaaatc aacagatctg ggtatttctg ttgatgtcta tactgtgatg agttatggtg
     3181 tgaaaatcag tgaagtctct aaaaatatcc aagaacgcgt gaaatttaac cttgaaagtc
     3241 agcttggtct cactgctgat atggtcaatg tttacgtaca aaatattaag gttgtaggag
     3301 aaaattagtg tcaaatatta caacaagctt attccaagaa atggtccaag cggcagcgac
     3361 tcgccttggc aaacaagcag aatatgttaa ttccttaaat gtttttcctg ttccagatgg
     3421 tgatacagga acaaacatga gcatgactat ggacaatggt gccaaggaag ttgctgataa
     3481 acctgcgtca actgtgggag aagttggaca aatgttatca aaaggtcttt tgatgggagc
     3541 gcgtggaaat tctggagtta ttacttcaca actttttcgt ggttttggtc aaagcattaa
     3601 gggaaaagat gagctgacag gtaaagattt ggcacaagcc tttcaagtgg gtgtagaagt
     3661 tgcctacaaa gcagtgatga aacctgttga aggaacaatt ttaactgttt ctcggggtgc
     3721 agcgactgct gccttaaaaa aagcagatct tacagacgac gccgtggaag tcatgcaagc
     3781 tgctttagac ggtgccaaag gtgcccttgc taagacacct gatttattac cagtccttaa
     3841 agaagttggt gtggttgatt caggcggtca aggtcttgtc tttatctatg aaggtttcct
     3901 atctgctttg aatggcgact atgtgacttc agcagacttt aaagcgactc ctgctaacat
     3961 gtctgaaatg attaatgctg agcatcacaa atccgttgtt ggtcatgtgg ctactgaaga
     4021 cattacctac ggttattgta ctgaaatcat ggttgccctt aaacaaggcc caacctatgt
     4081 gaaagaattc aactatgatg agtttcaagg ctaccttagt ggccttggtg actcgctgtt
     4141 agtggtcaat gatgatgaga ttgttaaagt tcacgtccat actgaagacc ccggacttgt
     4201 gatgcaagaa ggtcttaaat atggttcgct catcaagatt aaagtggaca atatgcgtaa
     4261 ccagcatgaa gcacaggttc aaaagactga tgtcgaaaaa accaaggctg aagtaaaaga
     4321 ctttggcttg attgcagtag tagctggaga aggtttatct gaaattttca aggcgcaagg
     4381 tgttgattac gtgatttctg gtggtcaaac catgaatcca tctacagaag atattgttaa
     4441 agctattgaa gcagttaatg ccaaacaagt cattatcttg ccaaacaata aaaatatctt
     4501 tatggctgcc caatctgctg cagaagtggt ggatatcccg gctgctgttg ttgcaacacg
     4561 tactgttccg caaggattta ccagcttgtt ggcctttgat ccttcaaaat ctttagagga
     4621 caatgtagca gatatgtcaa ctagtctttc tgacgtggtc agtggaagtg ttactttggc
     4681 agtccgtgac accaccattg atggtttaga aatccatgaa aatgatttct tgggaatggt
     4741 tgatggtaaa atcatcgttt caaacccaga catggaagca actctaaaag ctgcctttga
     4801 aaagatgatt gacgaagata gcgagattgt gactatcttt gttggtgaag aaggtgatca
     4861 agacttagca gaagaacttg ctgggtattt aggagagact tacgaagatg tggaagttga
     4921 gatccatcaa ggagatcagc ctgtttaccc atatctcatg agtgtagaat aatagaatac
     4981 aataaaaaaa gaggagttag gcaaaggtct gactcttttt gtctatattg atgtcgtaat
     5041 gagtggtttt agtgatggta agcccctaaa aaatgagggt aatccatcac aaagctaatg
     5101 gttacgattt aatttgacaa aacctagaaa aatattataa ttgtattata acaataagtc
     5161 aagagctgaa aacggttcag ttctaagaca tgacaaggag gagattttat gttaggacca
     5221 tttatcttta ttgcttttgg ggttattgta atcctagcta ttgttgccag taccctttat
     5281 gttgttcgtc aacaatctgt tgccatcgtg gaacgttttg gtaggtacca aaaaacagcc
     5341 actagtggta ttcatgtccg ccttccgttt gggattgaca aaattgctgc gcgtgtgcag
     5401 ctgcgtcttt tacagtcaga aatcatcgtt gaaaccaaaa ccaaggacaa tgtgtttgta
     5461 actttgaatg ttgccactca gtatcgtgtt aacgaacaaa atgtaacaga tgcttactac
     5521 aaattaatga aacctgaatc tcaaatcaag tcttatattg aagatgcctt gcggtcatcc
     5581 gtgccgaagt tgaccttaga cgaattgttt gagaaaaaag atgaaattgc cttggaagta
     5641 caacatcaag tggcagaaga aatgtcaacc tatggatata ttattgtcaa aaccttgatt
     5701 accaaggtgg agcctgatgc tgaagtcaag cagtcaatga atgaaatcaa tgcggctcaa
     5761 cgtaaacgtg tggctgccca agaattggca aacgcagata aaattaaaat tgtgacagca
     5821 gcagaagctg aggctgaaaa agatcgattg cacggggtag ggattgccca acagcgtaag
     5881 gccattgtgg atggtttggc ggagtctatt caagagctta aagaagcgaa tatttcctta
     5941 aatgaagaac aaatcatgtc cattttgttg accaaccaat acctggatac cctcaatacc
     6001 ttcgcggcca aaggaaacca gacccttttt ctaccaaata caccaagtgg ggtagaggat
     6061 atccgtacac aagttttatc tgcccttaaa acaaaataac gtgtaaaagc ttggctaaaa
     6121 agttgctcaa tactggattt agcagaaggc tatcatacct tgtgaagtgg caaacgacga
     6181 aatgaataca ttacttttat tggagaaatg aaagtaaaaa aagccttttt catgattaac
     6241 aacatagtat tgat
//
LOCUS       DAUCWU010000085         6170 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUCWU010000085 DAUCWU010000000
VERSION     DAUCWU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.085"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..452
                     /locus_tag="VGY24_001502"
     CDS             <1..452
                     /locus_tag="VGY24_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ4284469.1"
                     /translation="IADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEI
                     KALSGIDWDMEQRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAH
                     KIGDAYDYKHDVAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            449..1735
                     /gene="tilS"
                     /locus_tag="VGY24_001503"
     CDS             449..1735
                     /gene="tilS"
                     /locus_tag="VGY24_001503"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ4284470.1"
                     /translation="MKTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLTIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQIIQTA
                     KQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKEEKMLEYDSNLCYRDYCFSFMPKSN
                     GDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQ
                     DEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            1740..2282
                     /gene="hpt"
                     /locus_tag="VGY24_001504"
     CDS             1740..2282
                     /gene="hpt"
                     /locus_tag="VGY24_001504"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ4284471.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            2304..4283
                     /gene="ftsH"
                     /locus_tag="VGY24_001505"
     CDS             2304..4283
                     /gene="ftsH"
                     /locus_tag="VGY24_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ4284472.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            4609..6000
                     /locus_tag="VGY24_001506"
     CDS             4609..6000
                     /locus_tag="VGY24_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ4284473.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         2106 a          888 c         1136 g         2040 t
ORIGIN      
        1 aaatagctga taataaagat tatcgtgttg aagacctact gaaagctgta gcacaacaat
       61 cggataatgt agcaactaat attttaggtt attatctatg tcatcagtat gataaagctt
      121 tccgctcaga gataaaagct ttatcaggta tcgattggga tatggagcag cgcttattaa
      181 cttctcgttc agctgcaaat atgatggaag ctatttatca tcaaaaaggc caaattattt
      241 cttacctttc aaataccgaa tttgatcaac aacgtatcac aaaaaatatt actgttccag
      301 ttgcacataa aattggtgat gcttatgatt ataaacatga cgttgctatt gtttacggta
      361 atactccatt tattttgtct atttttacaa ataaatcaac ctatgaagat attacggcta
      421 ttgcagatga cgtttatggt attttaaaat gaagacctat caagaaattt ttaatgaaat
      481 aaaaaataaa gcctacttta aaaatcatag acatgtgtta atagccgtat ctggtggagt
      541 agattcaatg aatttattgc actttttata cctgtttcaa gacaaattga caattcgtat
      601 tggtattgct catgttaatc acaaacaaag atcagaatca gattcagaag aagcttattt
      661 aaagtgttgg gctaaaaaac atgacatacc tatttatgtt tctaattttg aaggaatatt
      721 ttcagaaaaa gcagcacgtg attggcgcta tgcttttttt aaaagtatta tgttaaaaaa
      781 taactattca gctttagtaa cagctcacca ttcagatgat caagcagaaa ctattttgat
      841 gaggctaatt agaggaagcc gattgaggca cttgtcgggt ataaaaagcg tgcaaccatt
      901 tgccaatggt caacttataa gacctttttt aactttctca aaaaaggact tgccagaaat
      961 ttttcatttt gaagattctt caaataggga attatcattt ttaagaaata gagttagaaa
     1021 taattattta ccattgttaa agcaggaaaa tcctaggttc attcagggat taaatcaatt
     1081 agcacttgaa aatagcttac tttttcaagc ttttaaggag ctaacaaatc atattactac
     1141 cactgattta acagaattta atgagcaatc taagtctatt caatactttt tgttacaaga
     1201 ttatttagaa ggttttccag atttagattt aaaaaaatct caatttactc aattattgca
     1261 aattatccaa actgctaaac aaggctatta ttatctcaaa aaagactatt atatattcat
     1321 tgataaattc tcttttaaaa ttacaaaaat cgttcctaag actgagttag ttaaagaaga
     1381 aaaaatgtta gaatacgata gtaacttatg ctacagagat tattgttttt catttatgcc
     1441 aaaaagtaac ggagatcaag gacaggtaag tataccttta tttagtttat cgtctattaa
     1501 attaagaagt cgccaatctg gagattatat ttcttttggt cacttttcaa aaaaaataag
     1561 acgtttattt atagatgaaa aattcacaat agctgaacgt caaaatgcta ttattggaga
     1621 gcaagatgag cagattatat ttgtattgat aggtaataaa acttatttga gaaaagcttg
     1681 taaacatgat ataatgttgg ctaaactgta tattgataaa ttagaaaaag ggtgacagca
     1741 tgctcgagca agatattcaa aaaattcttt actctgaaaa tgatattatt cggaaaacta
     1801 aaaaattagg agaacaatta acaaaagatt atcaagaaaa aaatccgctt atgattggtg
     1861 tattaaaagg atcagttcct tttatggcag aactaatgaa acacatcgat actcatgttg
     1921 aaattgattt tatggtagta tcaagttatc atggcggtac atctagcagt ggtgaagtta
     1981 aaattttaaa agatgtagat accaatattg aaggaagaga tatcataatt gttgaagata
     2041 ttattgacac cggtcgcacc ttaaaatatt tacgagatat gtttaagtac cgtaaagcta
     2101 atactatcaa aattgcaaca ctgtttgata aacctgaagg tcgtgttgtt aaaattgaag
     2161 cagattatgt ttgttacaat attccaaatg aatttatcgt aggctttggt ttagattacg
     2221 cagaaaatta tcgcaatctt ccttacgtag gtgtattaaa agaagaagtc tattcaaaat
     2281 agaaaaaggt caatgtttac tgtatgaaaa ataataaaaa taatggtttt gttaaaaatt
     2341 cttttatata tatattgatg attatagttg tcatcacagg atttcaattt tatcttaagg
     2401 gaaccagcac acaaagccaa cagattagtt attccaaatt aattaaacat cttaaggctg
     2461 gagatataaa atctttaagt tatcagccta gcggtagtat tattgaagtt aaagggaaat
     2521 acgagaaacc tcaaaaagtg acagttaatt caggactatc atttttaggt ggaagagctt
     2581 caacacaggt aacagagttc tcatctttag tattacctag tgataccatc ttgaaagaaa
     2641 tgacagcagc agctgataaa aatggaacag agcttacagt taagcaggaa agttcaagtg
     2701 gtacttggat taccttctta atgagctttt tacctattgt tatttttgct gctttcatga
     2761 tgatgatgat gaatcaaggt ggtggaggag ctcgtggtgc tatgagtttc ggcaaaaata
     2821 aggcaaaatc tcaatcaaaa ggtgatgtta aagtgagatt tacagacgtt gctggtgccg
     2881 aagaagaaaa acaagaatta gtagaagttg ttgatttttt gaaaaatcct aaaaaatata
     2941 aatcactagg agcacggatt ccagcgggag ttcttctaga ggggcctcca ggtactggta
     3001 aaacattact agctaaagct gtagcaggtg aagctggagt gccatttttc agtatttcag
     3061 gttcagattt tgtcgaaatg tttgtcggtg taggtgctag tcgtgttcgt tctttatttg
     3121 aggatgctaa aaaagctgag cgtgcgatca tctttatcga tgaaattgac gctgtaggtc
     3181 gtcgtcgtgg tgctggcatg ggaggcggta acgatgaacg agaacagacc cttaaccaat
     3241 tactcattga aatggatggt tttgagggta atgagaacat tatcgttatt gctgcaacta
     3301 accgtagtga tgttttagat ccagcgttac taagaccggg tcgttttgat cgtaaggttt
     3361 tagttggtcg ccctgatgtt aaaggacgtg aagccatttt acgtgttcat gctaaaaata
     3421 aaccacttgc aaatgacgtt aatttgaaag tagtagccca acagactcca ggttttgtag
     3481 gtgctgatct tgaaaatgtt ttaaatgaag ctgcgttggt tgctgctcgc agaaataaaa
     3541 ttaaaattga tgctagtgat attgatgaag ctgaagatcg tgttattgct ggaccatcaa
     3601 agaaagatcg cactatttcg caaaaagagc gagaaatggt tgcttatcac gaagctggtc
     3661 atactattgt tggtttagtt ttatctaatg cgcgtgtagt tcacaaggta actattgtac
     3721 ctcgtggacg agcaggtggt tatatgatcg ctcttccaaa agaagatcaa atgcttcttt
     3781 ctaaagaaga tctgaaagag caattagcag gattgatggg tggtcgtgtt gctgaagaaa
     3841 ttgtttttaa tgctcaaaca tcaggtgcgt caaatgactt tgagcaagct actcagatag
     3901 caagggccat ggttacggaa tatggtatga gtgaaaagct tggaccagtt caatatgaag
     3961 gtaaccatgc gatgatgcca ggacagatct ctcctgaaaa agcttactct gcccaaacag
     4021 ctcaaatgat tgatgatgag gttcgtgaac tcttaaatca agctcgtaat caagcagctg
     4081 atatcatcaa tgagaatcga gatactcata aattgattgc tgaagctctt ctcaaatatg
     4141 aaactctaga tgctgcacaa attaaatcaa tttatgaaac tggtaaaatg ccagttgatt
     4201 tagaaactga tgataatgaa gctcatgcgt tatcttatga cgaaattaaa aataaaatga
     4261 cagaatctga atctgcagaa tgatataaaa aagctgaact tatagattaa gttcagcttt
     4321 tttatcaaca caatatcctt gtaaacatta tgaattgtaa taggtgccta attgctagaa
     4381 atgagaagat gacatacttt ttttctttat tacttaaagt gagaaagact ttggtattta
     4441 aaactagaaa attgttaaag ccatttgaaa aaatctatta gtgttttctt agaataatag
     4501 aagaacagaa gatagcaaaa gtttcagtca accaataaga tgaaatttac aatcattact
     4561 aactatgata cacttagtag aaaatcattt actatagaag gtgataacat gactattttt
     4621 cgtaaaaaaa agaaatattc caataaaaca gaaatgcaaa ggcacttcaa agttattgat
     4681 ttagtttttt taggcctagg ttcaatggtt ggaacaggta tctttacagt tactggcata
     4741 ggcgctgcta agtatgcagg tcctgcttta acaatatcga ttatcatttc cgcgattgct
     4801 attggaattt tagccttatt ttatgcagaa tttgcgtctc gtatgccttc taacggtggt
     4861 gcttatagtt atgtttatgc aaccttggga gagtttccag catggcttgt tggctggtat
     4921 attattatgg aatttttaac agctatatct agtgttgctg ttggctgggg aagttattta
     4981 aaaggattac ttgccaatta taatatagta ttaccaaacg ctttaaatgg cacgttcaat
     5041 cttaagaatg gaacttacat agatattctt cctgttttag tgatgttttt cgtgacaggg
     5101 attgtcctaa tgaattcgaa attagccctg agattcaata gttttttagt tattttaaaa
     5161 ttttcagcac tggctttatt tatctttgta ggcatttttt ttattgatca caataattgg
     5221 tctcattttg ctccatatgg tgtaggacaa attactggag gtaaaacagg tatctttgct
     5281 ggtgcttctg ttatgttctt tgcattttta ggatttgaat ctatttcaat ggcagttgat
     5341 gaagtgaaag aaccacaaaa aacaattcct aaaggtatca ttttatcttt gattattgta
     5401 acagcactct atatagttgt caccactatt ttaacgggaa ttgtacacta cactaaatta
     5461 aatgttccag acgcggtggc ttttgcttta agaaatatta gactttattg ggcagctgac
     5521 tatgtgtcga tagttgctat tttgactctt attacggtat gtatttctat gacctatgca
     5581 ttagctagaa caatttacag tattagtaga gatggtttat tacctaaatc actttatact
     5641 ctcactaaaa aaaataaagt gcctcaaaat gctactcttg tcactggact acttgctatg
     5701 atttgtgcgg gaatatttcc tttatcgagt ctagcagagt ttgttaatat ttgtacttta
     5761 gcatatttaa ttattctgtc gggtgcaatt attaaactca gaagaataga aggagaacca
     5821 aaagcaaatg aatttaaaac tcctctagta ccattcctac caatgttggc aattatcatt
     5881 tgtctatcat ttatgagcca atataaagct tttacttgga ttgcttttgc tattgccaca
     5941 attattggaa cattaatcta cttagcttat ggttatactc attcaataga aaataaataa
     6001 tgttttgata ataagatttg acattacatt atagactata atgtttagaa gtaaatgaat
     6061 agtaaaatca atacctactt acttatcaaa gttgtaatat ttaaaattac ccaaccccta
     6121 gagaattgat tagtctctag gggttgattg gcacaatgac atagactcta
//
LOCUS       DAUCWU010000086         6138 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUCWU010000086 DAUCWU010000000
VERSION     DAUCWU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.086"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            33..1088
                     /gene="prsA"
                     /locus_tag="VGY24_001507"
     CDS             33..1088
                     /gene="prsA"
                     /locus_tag="VGY24_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ4284474.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKKAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            1475..4093
                     /gene="alaS"
                     /locus_tag="VGY24_001508"
     CDS             1475..4093
                     /gene="alaS"
                     /locus_tag="VGY24_001508"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ4284475.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEDKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFKYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            4453..4668
                     /locus_tag="VGY24_001509"
     CDS             4453..4668
                     /locus_tag="VGY24_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284476.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQAPVEEVFRLVEVEE"
     gene            4665..5426
                     /locus_tag="VGY24_001510"
     CDS             4665..5426
                     /locus_tag="VGY24_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ4284477.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            5445..>6138
                     /locus_tag="VGY24_001511"
     CDS             5445..>6138
                     /locus_tag="VGY24_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ4284478.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRSVDWGYLALFWLIIRVIAIVGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
BASE COUNT         1957 a         1069 c         1295 g         1817 t
ORIGIN      
        1 ttagttaaag ataaaaagga gctcagaagt aaatgaaaaa ctcaaataaa ctcattgcta
       61 gtgttgtgac attggcctca gtgatggctt tagcagcttg tcaatcaact aatgacaata
      121 ctaaggttat ttcgatgaaa ggtgatacaa ttagcgttag tgatttttac aatgaaacaa
      181 aaaacacaga agtatcgcaa aaagcgatgc taaatctggt aattagtcgt gtttttgaag
      241 ctcaatatgg tgataaggtt tcaaaaaaag aagttgaaaa ggcgtatcat aaaacagctg
      301 aacagtatgg cgcttcattc tctgctgctt tggcacaatc aagcttaaca cctgagactt
      361 ttaagcgtca gatccgctct tcaaaattag tagaatatgc ggttaaaaaa gcagctaaaa
      421 aagaattgac aacacaagaa tataagaaag catatgaatc ttatactcca acaatggcag
      481 tcgaaatgat tactttagat aatgaagaga cagctaaatc agtcttagag gaactaaaag
      541 ccgaaggcgc agactttaca gctattgcta aagaaaaaac aacaacacct gagaaaaaag
      601 tgacctataa atttgattca ggcgcgacaa atgtaccgac tgatgtcgta aaagcggctt
      661 caagtttgaa tgagggtggc atatcagacg ttatctcggt tttagatcca acttcttatc
      721 aaaagaagtt ttacattgtt aaggtgacta aaaaagcaga aaaaaaatca gattggcaag
      781 aatataagaa acgtttgaaa gctatcatta tagctgaaaa atcaaaagat atgaatttcc
      841 aaaacaaggt tattgcaaat gcattggata aagctaatgt aaaaattaaa gacaaagctt
      901 ttgctaatat tttggcgcaa tatgcaaatc ttggtcaaaa aactaaagct gcaagtgaaa
      961 gttcaacaac cagcgaatca tcaaaagctg cagaagagaa cccatcagaa tcagagcaaa
     1021 cacagacatc atcagctgaa gaaccaactg agactgaggc tcagacgcaa gagccagctg
     1081 cacaataagt aggattttat tgactaatca gttaaaaaaa cttataataa tttagttaag
     1141 aattgtttat caaggattag atacagaaaa gtgacggttg ctgcgagtca ttgataatta
     1201 gataaacatg ctacttaact ttacaacaat tgatatcgta aattaagaat gacaagttca
     1261 ttgaatagag gtggtaccgc ggttttcgcc ctctgtgaga tggacttgtt ttgtattgag
     1321 gactatttga aatcaacgaa aggaatagag tagactaggc tgccaggctt atagctgatc
     1381 tcctagcaag aaagacgagc tgtgtcaact ggtgtgatta cattactagc ttcagcataa
     1441 cactgttttt cttactcttt gtatctaaat ttttatgaaa gaattatcgt ctgcacaaat
     1501 ccgccaaatg tggttggatt tctggaaatc taaaggacat tgcgttgagc cttcagctaa
     1561 cttggttcct gtgaacgacc caacgcttct ttggatcaac tcaggtgttg caaccttgaa
     1621 aaaatacttt gatggttcag tgattccaga aaatccacgt attaccaatg cacaaaaatc
     1681 aattcgtact aatgatattg aaaatgttgg taaaacagca cgtcaccata ctatgtttga
     1741 aatgcttggt aatttctcaa ttggagacta tttccgtgat gaagctattg agtggggatt
     1801 tgaactcttg acaagtccag actggtttga tttccctaaa gacaagctct acatgactta
     1861 ttacccagat gacaaggatt cgtataaccg ttggattgct tgtggcgttg aaccaagtca
     1921 cttggtgcca atcgaggata acttctggga aatcggtgct ggtccttcag gtccagatac
     1981 ggagattttc ttcgaccgtg gtgaagattt cgatccagaa aatatcggac ttcgcctctt
     2041 ggctgaagat atcgaaaacg atcgttacat cgaaatctgg aacatcgttc tctcacaatt
     2101 caatgctgat cctgctgtgc cacgttcaga atataaagaa ttaccaaaca aaaacattga
     2161 tacgggtgct ggtcttgaac gtcttgcagc tgttatgcaa ggggcaaaaa caaactttga
     2221 aactgatctt ttcatgccta tcatccgtga agtagagaag ttgtcaggta aaacttataa
     2281 tccagatggc gacaacatga gtttcaaggt tatcgctgac cacatccgtg cgctttcatt
     2341 tgctatcggt gatggtgcgc ttcctggaaa tgaaggtcgt ggttacgttc ttcgtcgtct
     2401 tcttcgtcgt gcggttatgc acggtcgccg tcttggcatc aacgaaactt tcctttacaa
     2461 attggttcca actgttggac aaatcatgga aagctactac ccagaagtac ttgaaaaacg
     2521 tgactttatc gagaaaatcg ttaaacgtga ggaagaaaca tttgctcgta ctatcgatgc
     2581 aggtagcggt cacttagatt cattgcttgc gcagcttaaa gctgaagata aggatactct
     2641 tgaaggtaaa gatatcttca aactttatga tacttatgga ttcccggttg aattgacaga
     2701 ggaattggca gaagatgcag gctacaagat tgaccacgaa ggctttaagt cagccatgaa
     2761 agaacaacaa gaccgtgcgc gtgcagctgt tgttaagggt ggttcaatgg ggatgcaaaa
     2821 tgaaacccta gctggtattg ttgaagaatc acgattcaaa tacgacacat atagtcttga
     2881 atcaagtctt tcagtcatca tcgctgataa tgaacgtacc gaagctgttt cagaaggtca
     2941 agctcttctt gtctttgctc aaacaccatt ctatgctgaa atgggtggac aggttgctga
     3001 cacaggtaga atcaaaaatg ataagggtga cacagttgct gaggttgttg atgttcaaaa
     3061 agcaccaaat ggtcaacctc tacacactgt aaacgtttta gcatcacttt cagttggaac
     3121 aaactacaca cttgaaatca acaaagagcg tcgtttggct gttgagaaaa accacacagc
     3181 aactcacttg ctccatgcag cccttcacaa tgttatcggt gaacacgcaa ctcaggctgg
     3241 ttcattgaac gaagaagaat tcttgcgctt tgattttact cactttgaag cagtaagcaa
     3301 tgaggaactt cgtcacattg aacaagaagt taatgagcaa atttggaacg ctcttacaat
     3361 cacaacgact gaaactgacg ttgaaaccgc aaaagagatg ggagcaatgg cactttttgg
     3421 tgagaaatat ggtaaagtgg ttcgtgtggt tcaaattggt aattattctg ttgaactttg
     3481 tggtggaact cacttaaata attcttcaga aatcggtctc ttcaagattg tcaaagaaga
     3541 aggtattggt tcaggcactc gtcgtattat tgcagttact ggtagacaag cttttgaagc
     3601 ttatcgtaac caagaggatg ccctaaaaga gatcgctgct actgtaaaag ctccgcaatt
     3661 gaaagatgca gcagctaaag tacaagctct tagcgactca cttcgtgatt ttcaaaaaga
     3721 aaatgcagag cttaaagaaa aagcagcagc tgcagcagct ggtgatgtct ttaaagatgt
     3781 tcaagaagct aagggcgtgc gcttcattgc tagtcaagtt gatgttgcag atgcaggggc
     3841 acttcgtaca tttgctgata actggaaaca aaaagactac tctgatgtgc ttgttctcgt
     3901 agcagctatt ggtgagaagg ttaatgtcct tgttgcaagt aaaactaaag atgtccacgc
     3961 tggtaacatg attaaagaat tggcaccaat tgtagcaggt cgtggtggag gtaaaccaga
     4021 catggctatg gcaggtggta gcgatgcaag taaaattgca gagctgctag cagcagttgc
     4081 tgaaacagtg taaatccata aaaaccaagt tatttagaac ttggtttttt atatttgaaa
     4141 tgactatcat cataaccttt ttgaccatta tcgttgtcaa aaagaaaaag atatgttaca
     4201 atgatagtaa caaatagaca tgaggaggtg ttagagaaaa tgacacatgg tatcaaactt
     4261 aagaggcaga tttgcttatg ggcaagtatc ctgactcttt tcattttaag ttatcatctt
     4321 atcagggtaa tctggtttgg agtacccttg agttatctgg ttttgggctt tacaggacta
     4381 gccttgctgt tgaatacttt ttcttatttt agaaagtaag ttataagtta aagaagaatg
     4441 aaggagatgg caatggaggt tattcttaaa aatcggctca aagaattacg agccagagat
     4501 ggaattaatc aaacggaaat ggctaaactg gcaggagtat cgaggcagac catcagtctc
     4561 attgagcgta acgagtacac cccatcagtt ataattgcca tgaaaattgc aaaggtattt
     4621 caggcaccag ttgaagaggt gtttcgtcta gtggaggtcg aggaatgaaa aagaagaaga
     4681 atagtttttt acgcttgtta aaaatgttat tgctatcaag tttggcggga gggattatag
     4741 gtggcatggt tggagccttt ttaggttacc atggagagag attagatcag ctcacgttcc
     4801 taaaagatga tgtgattaac ctgattatct tattaaatcg tctagtagtt gtcacaggcc
     4861 tcacactttc ttttgttttt ttaacgcaac tgaaaaaaga gacagctgtt tataacacaa
     4921 ttgaagagga tgattattct gaaaatggtt accgtcagct gaataaaaag catgcttata
     4981 ccatgttatt gattgccgta gcttctattt tatccatgtg caatgtgcta cttggtctga
     5041 ccttaactaa tgattcacag catgccatgc tggccattcc gcttctggat atccttttat
     5101 tattaatggt cattcctttt caagctttag ccatgaaacg ttacaatgcc attcggggaa
     5161 ccgatgttcc ttattttcca aatttgaaag aattaaaaca taatatcatg gctctagatg
     5221 aggcagagtt acaagcctat cataagacat cgtttgaaag tgtgttgtca ttaaatggag
     5281 tcatcatccc ttccttgtat gtgattttat tttttgtcta tctattcact ggtcaagtgg
     5341 agttaacagc catcttagtt ttggtgttaa ttcagctcta tttactcgtt aagagtgcaa
     5401 caatgaccag acaattttac cgttagccta aaaggagact gtttatgaaa ggattcatta
     5461 attatttaaa aatagcagtg ctcattattc tggctatggt tttcaatgtc cttccgatga
     5521 ttttattgca aaagcaacac gatattccta tggtacttaa ttggggaatt ggtattttct
     5581 acttggttat tgttggaagt gtccttattg tattatggcg tctttatcaa gctaagcaag
     5641 acacccctat taaacagcaa aaaatgagat cggttgactg gggttattta gcattatttt
     5701 ggttaatcat ccgtgtgata gctattgtag gtacccttgt taaccagcta tggtctggtc
     5761 aacaagtgag tgctaatgat gctgcaatac ataccttagc tagacttatc aaaggtggtt
     5821 tcccgcttta tacggcccta tttgtacttg tgatagcttt tatcgctcct attatggaag
     5881 aactagtctt tagaggattt cctatgattg atctcttcaa aggaaaatca cttaaggtgg
     5941 caggtttagt gacctctctt gtttttgctt taccacatgc caccaatagt gttgaattta
     6001 tcatgtacag ctgtatgggc atttttctct ttgttgccta tcaaagacga ggaaacttaa
     6061 aagacgctat cttgttacat atttttaata acttgattga agtcattttg ttaatgtcaa
     6121 taggcttagg agtcatat
//
LOCUS       DAUCWU010000087         6087 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUCWU010000087 DAUCWU010000000
VERSION     DAUCWU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.087"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            388..>6087
                     /locus_tag="VGY24_001512"
     CDS             388..>6087
                     /locus_tag="VGY24_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ4284479.1"
                     /translation="MFLKHQDVKQKNWRMRKVKKLFVSSCMLLTVGLGVAVPTAFSQS
                     NGVMVVKAAEVSEEVSKLPTVSFVKQNPEGSDTSKRVLWGPEVDKTYSKHAAGSDQRK
                     YYDNDVKAPVLRVGILGSSTNGGIEISAPVYSNTNYTLRVNLAHQMTWVRLGYKGAVK
                     TGEYYEKTKSNKLYTHEMRLVTSNKKHVYQGEGSEGKLTMYVYRDNRVDGKNNSAAII
                     KDAILVPDTPKLVPQNKELELKLSKVENKSIQSIVNEIPEVSKILDSLVTDSAHFNNP
                     VKSTWSKIDNPDKVITTVDDSFVLNYTAEKWGQNTSGKVTVRLIEDRDYKKDIDLAKQ
                     SLLDIKKKTIKQIDLDNWLSDDEKRDQTSEVERTYTEGESEINKAKTLTTIHDVLKKY
                     STEGNNISIPDQYKKGKKEAKVSQEKEKLQSVYDTEKNKIVQDKWSSTSQKQEEQRLL
                     DSVKTEAENNLSETNSLQDLLSKSPSEQDKLRKIYKGNKEQKVREDGLKNLKKTLLDT
                     QIAIKEDNWLSNGAKKMQLTAAQKAFEKGAKELQNSQDFATHEEKFNNYVSKDTEKHP
                     DSIPNQYIPGNQEEIKSKEKVNLKKVYDDTISNINQDNWLSDEDKQKQTAQATQAYEN
                     GIQDISSADSLINLKQVVEKSVGNEDETSIPNKHIKGDQKKEVDSATQKLQEIHKKTQ
                     DAIETDPWLSPEQKKAQKENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKAESIP
                     SQHQAGTPEVARKTFLDNFEKEAKKEIESIDKDDTLTANAKQVAKDKVAQQLQEATAK
                     VEKAQSFDDLKKVETEFVPALPHTKGEKLNQQQTEAISGLEGVQKATEKAISEDKTLS
                     KDEKKKQIADSKERLKSDTQKVKDAKNADAIKKAFEEGKVNIPQAHIPGDLNKDKEKL
                     LAELKQKADDTEKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDEKVP
                     ELKKAIEDTHVKGNLEGVKNKAIEDLKKVHDETVAKINGDVTLDKATKEAQVKEADKA
                     LAAGKEAITKADDADKVSTAVTEHTPKIKAAHKTGDLKKAQEEANTALDKAAEKEREE
                     INNDITLTAKDKEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQ
                     DLGARKSGQVAKLEEAAKETKDKISADPTLTSKEKEEQSKAVDAELKKAIEAVNAADT
                     ADKVDDALGKGVTDIKNQHKTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEK
                     AKQVKDVDAAKERGMAKLNEAKDADALDKAYGDGVTSIKNQHKSGDPVEARRGLHNKS
                     IDEVAQATKDAITADTTLTEAEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGV
                     TSIKNQHKSGKGLDVRKDEHKKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELE
                     LATDKIAEAKDADEADKAYGDGVTAIENAHVIGKGIEARKDLAKKDLAEAAAKTKALI
                     IEDKTLTDDQRKEQLLGVDTEYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQN
                     LNDRRNAAKEFLLKEADKVTKLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTA
                     DAINDALGKGIENINNQYQHGDGVDVRKATAKGDLEKEAAKVKALIAKDPTLTQADKD
                     KQTAAVDAAKNTAIAAVDKATTADGVNQELGKGITAINKAYRPGEGVKARKEAAKADL
                     EKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTAEGINQELGKGIT
                     AINKAYRSGEGVKARKEAAKADLEKEAAKVKALITNDPTLTKAAKAKQTEAVAKALKA
                     AIAAVDKATTAEGINQELGKGITAINKAYRPGEGVKARKEAAKANLEREAAKVREAIA
                     "
BASE COUNT         2401 a          995 c         1281 g         1410 t
ORIGIN      
        1 ggtgacgcgt gtttaaggag tattgagaat gtcagatata agcaaaatgc agctgaatat
       61 tcttaatttt aggtatgaaa aagcgtcggg attgataaaa aatctattga attgggtaaa
      121 tttaagttga tataataagt tgattagaaa aattaacaat tcatttaaat tctaatcagc
      181 tgtaacgttc cggggcgtgt taaactgtct gaacatagaa aatacaggtt tctacggtga
      241 ctaagataag ttaaggagga ttccttaaat tctgtgatag gaacgtaaac tctctacaag
      301 agtagagcac gtcatcagtc agtctgggtg tctactggct gacaacgtca aataagatag
      361 gcatgaaaaa ataaggggga atacagaatg tttttaaaac accaagatgt gaaacaaaaa
      421 aattggagaa tgcgtaaggt caaaaaactc tttgttagtt catgtatgct tttaacagtc
      481 ggcctcggag ttgccgtacc tactgcattc agccaatcta atggcgtgat ggtcgtaaag
      541 gctgcggaag tttcagaaga agtaagtaaa ttgccaactg ttagttttgt caaacaaaat
      601 ccagaaggtt ctgatacctc aaagcgggtt ttatggggac cggaagttga caaaacttat
      661 tcaaagcatg ctgcaggatc tgaccagaga aaatattatg ataatgatgt gaaagcacca
      721 gttcttagag taggaattct tggtagttct acaaatggag gtattgaaat atctgcgcct
      781 gtttatagta atactaatta tactctaaga gttaatttgg cacatcaaat gacctgggta
      841 agattaggct ataaaggtgc tgtgaaaact ggagaatatt acgaaaaaac caaaagtaac
      901 aaactttata ctcatgagat gagattagta acttcaaaca agaaacatgt ttatcaaggt
      961 gaaggttctg aaggaaaatt aaccatgtat gtttataggg ataacagagt tgatggaaaa
     1021 aataattcag cagccatcat taaagatgct attttagttc cagatactcc taaattagtc
     1081 cctcaaaata aagaattgga attaaagtta tcgaaagtag aaaataaatc tatacaatca
     1141 atagtaaatg aaataccaga agtttcaaaa attcttgatt ctctagttac tgatagcgcc
     1201 cattttaata atcctgtcaa atctacttgg tctaaaattg ataatccaga taaagtgata
     1261 actactgttg atgattcttt tgttcttaat tatactgctg aaaaatgggg gcaaaatact
     1321 tcgggtaagg ttactgttcg cttgatagag gatcgtgact ataaaaaaga tattgattta
     1381 gctaaacaat cgttattaga tattaagaaa aaaacgatta agcaaataga cttagataat
     1441 tggctgtcag atgatgaaaa gcgagatcaa acttcagaag ttgaaagaac ttatactgaa
     1501 ggtgagtctg aaattaacaa ggcaaaaaca ttgacaacaa tacatgatgt cttgaaaaag
     1561 tattctaccg agggaaataa catctctata cctgatcaat ataaaaaagg taaaaaagaa
     1621 gcaaaagtct ctcaggaaaa agaaaaatta caatctgtct atgatacaga gaagaataaa
     1681 attgtacaag ataagtggtc tagcacaagt cagaagcaag aagaacaacg attattagat
     1741 tctgttaaaa cagaggcaga gaataattta agtgagacga acagtttaca ggatttgtta
     1801 tcaaaatctc cgtcggagca ggataaatta cgtaaaattt ataagggaaa taaagagcaa
     1861 aaagttcgtg aagatggctt gaagaacctt aaaaaaacat tgttagacac tcaaattgct
     1921 attaaagaag ataattggct ctcaaacgga gctaaaaaaa tgcaattgac tgcagctcaa
     1981 aaagcatttg aaaagggagc taaagaactc caaaattcac aagattttgc tacacatgaa
     2041 gaaaagttta ataactatgt aagtaaagat acagagaaac accctgattc aattcctaat
     2101 cagtatatac ctggaaatca agaagagatt aagtctaaag aaaaagttaa tttgaaaaaa
     2161 gtttatgatg atacaatctc aaatattaat caagacaatt ggttgagtga tgaagataaa
     2221 caaaaacaaa ctgctcaggc aactcaagct tatgaaaatg gaattcagga tattagttct
     2281 gctgatagtt taattaattt gaaacaagtt gtggaaaaat ccgttggtaa tgaggatgaa
     2341 acatctattc caaacaagca catcaaaggc gatcagaaaa aagaagttga ctcagcaacg
     2401 caaaaactcc aagaaattca taaaaagact caggatgcta ttgaaacaga tccatggttg
     2461 agtccggaac agaaaaaagc tcaaaaagaa aacgctaaag caagattaga tgcaggtttg
     2521 aaagctgtcg aaactacaga aagcttagat aaacttaaag aagttgagag tgacttcctt
     2581 gataaagaaa aagcagaatc tattccatct caacatcaag caggaactcc tgaagttgct
     2641 cgcaaaactt tcttagataa ctttgaaaaa gaagctaaaa aagagattga atctattgat
     2701 aaagatgata ccttaacggc aaatgctaaa caagtagcta aagataaagt ggcacaacag
     2761 ctacaagaag ctacagctaa agtagagaaa gcacagtcgt ttgatgattt gaagaaagtt
     2821 gagactgaat ttgtacctgc tttaccgcat acaaaagggg aaaaacttaa tcaacaacaa
     2881 actgaagcta tttctggttt agaaggagtt caaaaggcaa cagaaaaagc tatctcagaa
     2941 gataagacac tgtcaaaaga tgaaaaaaag aaacaaattg ctgactctaa ggaacgtttg
     3001 aaatctgaca cgcaaaaagt taaagatgct aaaaatgctg atgctattaa aaaagcattt
     3061 gaagaaggga aagtgaatat tcctcaagca catatcccag gtgatttgaa caaggataaa
     3121 gaaaaacttc ttgcagagtt gaagcaaaaa gcagatgata ctgaaaaagc gattgattcc
     3181 gataaaactt tgacagcgaa agaaaaagaa gagcaaaaag cgaaaacaaa agaagaactt
     3241 caaaaagcaa cagaagctgt tggagcaatt gacaatcgtg aagaactaga tgaaaaagtc
     3301 ccagaactta agaaagctat tgaagacact cacgttaaag gtaatcttga aggtgttaag
     3361 aataaggcta ttgaagatct taaaaaagtt catgacgaaa cagttgctaa gataaatggt
     3421 gatgttaccc ttgacaaagc tactaaagaa gctcaagtga aagaagctga caaagctttg
     3481 gcagcaggta aagaagctat cactaaagca gatgatgctg ataaagtaag tacagctgtt
     3541 acagagcaca caccaaaaat taaagcagca cataaaactg gtgatcttaa aaaagctcaa
     3601 gaagaggcta acacagctct tgacaaagca gctgaaaaag aacgtgaaga aatcaataat
     3661 gatatcacat taacggcaaa agacaaagaa caacaactga aagaagttga gacagctctt
     3721 actaaagcta aagataacgt gaaagctgct aagacagcag acgctatcaa tgacgcacgt
     3781 gataaaggcg tagcaacaat tgatgccgtc cataaagcag gtcaagactt aggtgctcgt
     3841 aagtcaggtc aagtcgctaa acttgaagaa gcagctaaag aaacgaaaga caagatttca
     3901 gctgatccaa ctttgacaag caaagaaaaa gaagagcaat ctaaagctgt tgacgctgaa
     3961 cttaagaaag cgatagaagc tgttaacgca gctgacacag ctgacaaggt tgacgatgct
     4021 cttggtaaag gtgtcacaga catcaagaac caacataaga caggtgaccc tgtcgtagct
     4081 cgtcgtgagg ctcatggtaa gcaacttgat agagtcgctc aagaaactaa agatgcgatt
     4141 gaaaaagacc ctactttgac gactgaagaa aaagctaaac aagttaaaga cgtagatgcc
     4201 gctaaagaaa gaggcatggc taagcttaat gaagctaaag atgctgatgc tttagacaaa
     4261 gcgtacggtg acggtgtgac cagcatcaag aaccaacaca agtcaggtga ccctgtcgaa
     4321 gctcgtcgtg gattacacaa caagtcaatc gacgaagtgg cgcaagcaac taaggacgct
     4381 atcacagcag atacgacttt gactgaagct gaaaaagaaa cacaacgtgg caatgttgat
     4441 aaagaagcaa ctaaagctaa agaagaactt gctaaggcta aagatgctga tgctttagac
     4501 aaagcgtacg gtgacggtgt gaccagcatc aagaaccaac acaagtcagg taaaggtctt
     4561 gacgttcgta aagatgagca caagaaagct cttgaagctg ttgctaaacg tgtcactgct
     4621 gaaattgagg ctgatccaac cttaacacca gaagtgagag aacaacaaaa agcagaggtt
     4681 caaaaagagc ttgaacttgc gactgataag attgctgaag ctaaagatgc agatgaagca
     4741 gacaaagctt acggtgacgg tgtcacagcg atcgaaaatg cccacgttat tggtaaaggt
     4801 atcgaagctc gtaaagacct tgctaagaaa gaccttgctg aagctgctgc taagacaaaa
     4861 gctctcatta ttgaagacaa aacgcttact gatgatcaac gtaaagaaca gttattaggt
     4921 gttgatacag agtatgctaa aggtatcgag aatattgatg cagctaagga tgctgcaggt
     4981 gttgataaag catatagtga cggtgttcgt gacatcctgg cacagtacaa agaaggtcaa
     5041 aaccttaatg atcgtcgtaa tgctgccaaa gaatttcttc ttaaagaagc agacaaagtg
     5101 acgaaactaa tcaatgatga tccaaccttg actcatgacc aaaaagttga tcaaatcaac
     5161 aaagttgaac aagctaagtt agacgcaatc aagtctgttg atgatgctca aacagctgat
     5221 gctatcaatg atgctcttgg taagggtatt gaaaacatca acaaccaata ccaacatggc
     5281 gatggcgttg atgttcgtaa agcgactgcc aaaggcgatc ttgaaaaaga agctgctaaa
     5341 gtgaaagctc ttattgctaa ggatccaacc ttgactcaag ctgataaaga caaacaaaca
     5401 gcagcggttg acgcagctaa gaacacagca attgcagcgg ttgataaagc gacaacagca
     5461 gatggtgtta accaagaact tggtaaaggc atcacagcta tcaacaaagc ttaccgtcca
     5521 ggtgaaggtg ttaaagcacg taaagaagcc gctaaagctg atcttgaaaa agaagctgct
     5581 aaagtgaaag ctcttattgc taaggatccg accttaactc aagctgataa agacaaacaa
     5641 acagcagcgg ttgacgcagc taagaataca gcaattgcag cggttgataa agcgacaaca
     5701 gctgaaggta ttaaccaaga actgggtaaa ggcatcacag ctattaataa agcttaccgt
     5761 tcaggtgaag gtgttaaagc acgtaaagaa gccgctaaag ctgatcttga aaaagaagct
     5821 gctaaagtga aagctcttat tactaacgac ccaaccttaa caaaagctgc taaagctaaa
     5881 caaacagaag ctgttgctaa agctcttaaa gctgctatcg cagcggttga taaagcgaca
     5941 acagctgaag gcattaacca agaacttggt aaaggcatca cagctatcaa caaagcttac
     6001 cgtccaggtg aaggtgttaa agcacgtaaa gaagctgcta aagctaatct tgaaagagaa
     6061 gctgctaagg ttcgtgaagc tatcgct
//
LOCUS       DAUCWU010000088         6053 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUCWU010000088 DAUCWU010000000
VERSION     DAUCWU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.088"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            112..3222
                     /locus_tag="VGY24_001513"
     CDS             112..3222
                     /locus_tag="VGY24_001513"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ4284480.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDILTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            3302..4315
                     /gene="pfkA"
                     /locus_tag="VGY24_001514"
     CDS             3302..4315
                     /gene="pfkA"
                     /locus_tag="VGY24_001514"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ4284481.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKDGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            4378..5880
                     /gene="pyk"
                     /locus_tag="VGY24_001515"
     CDS             4378..5880
                     /gene="pyk"
                     /locus_tag="VGY24_001515"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ4284482.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         1880 a         1034 c         1304 g         1835 t
ORIGIN      
        1 tttatgccat ttttcactct ttgagaagta gacgtagatt agagagaaaa ttgaagcaag
       61 aaaacctttt tcatagtata atagggaaca agataaccaa aggaggaact tatgtttgct
      121 caacttgata ctaaaactgt atactcattt atggatagtt taattgactt aaatcattat
      181 tttgaacgag caaagcaatt tggttaccac accataggaa tcatggataa ggataatctt
      241 tatggtgctt accattttat taaaggttgt caaaaaaatg gactgcagcc agttttaggt
      301 ttggaagtag agattctcta tcaagagcgg caggtgctcc ttaacttaat cgcccagaat
      361 acacaaggct atcatcagct tttaaaaatt tccacggcaa aaatgtctgg caagcttcat
      421 atggattacc tctgccaaca tttggaaggg atagcggtta ttattcctag taagggttgg
      481 agcgatacat tagtggtccc ttttgactac tatatcggtg ttgatcagta tactgattta
      541 tctcatatgg attctaagag gcagcttata cccctaagga cagttcgtta ttttgcgcaa
      601 gatgatatgg aaaccttgca catgttgcat gccattcgag ataacctcag tctggcagag
      661 acccctgtgg tagaaagtga tcaagagtta gcagattgtc aacaactaac caccttctat
      721 caaacacact gccctcaagc tctacagaat ttagaagact tagtgtcagg aatctattat
      781 gatttcgata caaatttaaa attgcctcat tttaatagag ataagtctgc caagcaagaa
      841 ttgcaagact tgactgaggc tggtttgaag gaaaaaggat tgtggaaaga gccttatcaa
      901 tcgcgcttac tgcatgaatt ggtcattatt tctgacatgg gctttgatga ttattttttg
      961 attgtgtggg atttacttcg ctttggacgc agtaaaggct attatatggg tatgggacgt
     1021 ggctcggcgg caggtagtct agtggcttat gctctgaaca ttacagggat tgatccagtt
     1081 caacatgatt tgctatttga gcgcttttta aacaaagaac gttatagcat gcctgatatt
     1141 gatatcgatc ttccagatat ttaccgttca gaatttctac ggtatgtccg aaatcgttat
     1201 ggtagcgacc attcggcgca aattgtgacc ttttcaacct ttggccctaa acaggctatt
     1261 cgtgatgttt tcaaacggtt cggggttcca gaatacgaac tgaccaatct cactaaaaaa
     1321 attggtttta aagatagctt ggctactgtc tatgaaaagt caatctcttt taggcaggtt
     1381 attaatagta gaactgaatt tcaaaaggcc tttgctattg ccaagcgtat cgaaggaaat
     1441 ccaagacaaa cgtccattca cgcagctggt attgtgatga gtgatgatat cttgaccaat
     1501 catattcctc taaaatcggg cgatgacatg atgatcaccc agtatgatgc tcatgcggtc
     1561 gaagctaacg gcctgttaaa aatggatttt ttggggttaa gaaatttgac ctttgttcaa
     1621 aaaatgcaag agaaggttgc taaagactac gggtgtcaga ttgatattgc agccattgat
     1681 ttagaagacc cgcaaacgtt ggcacttttt gctaaagggg ataccaaggg aattttccaa
     1741 tttgaacaaa atggtgctat taatctttta aaacggatta aaccacaacg ttttgaagaa
     1801 attgttgcca ctaccagtct aaatagacca ggggcaagtg actataccac taatttcatt
     1861 aaacgaagag aaggacaaga aaaaattgat ttgattgatc ctgtgattgc tcccatttta
     1921 gagccaactt acggtattat gctttatcaa gaacaagtta tgcagattgc acaggtttat
     1981 gctggtttta cgttaggcaa ggccgacttg ttaaggcgtg ccatgtctaa aaaaaatcta
     2041 caagaaatgc aaaaaatgga agaagacttt attgcttctg ctaagcacct agggagagct
     2101 gaagaaacag ctagaggact ttttaaacgg atggaaaaat ttgcaggtta tggttttaac
     2161 cgcagccatg cctttgccta ttcagcttta gcttttcaat tggcttattt caaagcccat
     2221 tacccggctg ttttttacga tatcatgatg aattattcta gcagtgacta tatcacagat
     2281 gctctagaat cagattttca agtagcgcaa gttaccatta atagtattcc ttacactgat
     2341 aaaattgaag ctagcaagat ttacatgggg ctgaaaaata ttaaggggtt gccaagggat
     2401 tttgcttatt ggattatcga gcaaagacca tttaatagcg tagaggattt tctcactaga
     2461 actccagaaa aatatcaaaa aaaggttttc cttgagcctc tgataaaaat aggtctgttt
     2521 gattgctttg agcctaaccg taaaaaaatt ctggacaatt tggatggttt actggtattt
     2581 gttaatgagc ttggttctct tttttcagat tcttccttta gttgggtaga tacgaaagat
     2641 tactcagcaa ctgaaaaata ttctttggaa caggagatcg ttggggttgg catgagcaag
     2701 catcctttaa ttgatattgc tgagaaaagt acccaaactt ttactcccat ttcacagtta
     2761 gtcaaagaaa gcgaagcagt cgtactgatt caaatagata gcattaggat cattagaacc
     2821 aaaacaagtg ggcagcaaat ggctttttta agtgtgaatg acactaagaa aaagctcgat
     2881 gtcacacttt ttccacaaga gtatgccatt tataaagacc aattaaaaga aggagaattc
     2941 tattacttaa aaggtagaat aaaagaaaga gaccatcgac tgcagatggt gtgtcagcaa
     3001 gtgcaaatgg ctattagtca aaaatattgg ttattagttg aaaaccatca gtttgattcc
     3061 caaatttctg agattttagg tgcctttcca ggaacgactc cagttgttat tcactatcaa
     3121 aaaaataagg aaacaattgc attaactaag attcaggttc atgtaacaga gaatttaaag
     3181 gaaaaacttc gtccttttgt tctgaaaacg gtttttcgat aaaaatggac aattagtgaa
     3241 gtattttgca aatgaatgtg ctataataag accgttaaat taattaaagg agtaccagaa
     3301 aatgaaacgt attgctgttt taaccagtgg cggcgacgct cctggtatga acgctgctat
     3361 tcgtgcagtc gttcgtaaag caatttcaga aggaatggaa gtttatggta tcaaccgtgg
     3421 ctatgcaggt atggttgatg gcgacatttt cccactgggt tcaaaagaag ttggtgataa
     3481 aatctcacgt ggtggaactt tcttatactc agctcgttat ccagagtttg ctcaacttga
     3541 aggtcaattg gctggtattg agcaacttaa aaaacatggt attgagggtg ttgttgttat
     3601 tggtggggat ggttcttatc atggcgctat gcgcttgaca gagcacggct tcccagcggt
     3661 aggtatccca ggaacaatcg ataacgatat tgcgggaact gactacacta tcggatttga
     3721 tacagcagtt aacacagcag ttgaagctat tgataaactt cgtgacacct catcaagtca
     3781 cggacgtact tttgtggttg aagtgatggg tcgcaatgcg ggtgatattg ctctttgggc
     3841 aggtattgct agtggtgctg atcaaatcat tgttccagaa gaagagtttg atattgaaaa
     3901 agtggcatca acaatccaat atgattttga acacaaaggt aaaaatcacc acattatcgt
     3961 cttagctgaa ggtgttatga gcggtgaagc ttttgctcaa aaactgaaag aagctggcga
     4021 taagagtgac cttcgtgtga caaatcttgg ccacattctt cgtgggggtt caccaactgc
     4081 tcgtgaccgt gtcattgctt cttggatggg ttcacatgct gttgagctac ttaaagatgg
     4141 caaaggtggt ttggctgttg gtattcacaa tgaagaactt gttgaaagcc caatccttgg
     4201 tactgcagaa gaaggtgctt tgttcagctt gactgaagaa ggcaagatta ttgttaataa
     4261 tccacacaaa gctcgtttag actttgcagc tttgaaccgt tcattgtcac aataagttta
     4321 gttaaaattg ttaaatcgtc aattattgac aaaaaaataa gggagtttca gagtaaaatg
     4381 aataaacgcg taaaaatcgt tgcaacactt ggtcctgcgg ttgaaatccg tggtggtaaa
     4441 aaatacggag aagatggcta ctgggctggt caacttgatg ttgaagaatc agcaaaaaaa
     4501 atcgctgaat taattgaagc tggtgcgaat gttttccgtt tcaatttctc acatggtgac
     4561 cacaaagaac aaggtgaccg tatggcaact gttcgccttg cagaagaaat tgctcgtcaa
     4621 aaagttggtt tccttttgga tactaaaggt cctgaaatgc gtacggaatt gtttgcagat
     4681 gatgctaaag agtttagcta tgttactgga gaaaaaatcc gtgtagcgac tacacaaggt
     4741 attcaatcaa ctcgcgatgt tattgctttg aacgttgctg gtagccttga catctacgat
     4801 gaagtagaag taggtcatac tattcttatc gatgatggta aacttggttt gaaagtgatc
     4861 gacaaagata tcgcaacacg tcaatttatc gttgaagtag aaaacgacgg tattattgct
     4921 aaacaaaaag gtgttaatat ccctaacact aaaattcctt tcccagcact tgcggaacgc
     4981 gacaatgctg atatccgttt tggtcttgaa caaggactta actttattgc aatctcattt
     5041 gttcgtacag ccaaagacgt tgaggaagtt cgtgaaatct gtcgcgaaac tggtaacgac
     5101 catgtgcaat tatttgctaa gattgaaaac caacaaggta tcgataatct tgatgaaatc
     5161 atcgaagctg ctgatggtat catgattgca cgtggcgaca tgggtatcga agtaccattt
     5221 gaaatggttc cagttttcca aaaaatgatt atcactaaag tgaatgcagc tggtaaagca
     5281 gttatcacag caacaaacat gcttgaaaca atgactgaaa aaccacgtgc gacacgttct
     5341 gaagtatctg acgtcttcaa tgctgttatc gatggtactg atgcaacaat gctttcagga
     5401 gagtcagcta acggtaaata cccggttgaa tctgttcgta caatggcgac tattgatcgt
     5461 aatgcacaaa ctcttcttaa cgaatatggt cgtttagatt catctgcatt cccacgtaca
     5521 aacaaaactg atgtgattgc ttcagcggtt aaagatgcca ctcattcaat ggacatcaaa
     5581 cttgttgtga ctattactga gacaggtaat acagctcgtg cgatttctaa attccgtcca
     5641 gatgcggaca tcttagctgt aacatttgat gaaaaagtac aacgtgcttt gatgattaac
     5701 tggggtgtta ttcctgttct tgctgaaaaa cctgcatcta ctgatgacat gtttgaagta
     5761 gcagagcgtg ttgctgtgga ggcaggctta gttcaatctg gggataatat tgttattgtt
     5821 gctggtgttc cagttggtac tggtggaacg aatactatgc gtgttcgtac agtaaaataa
     5881 atgtgttata actatttacc taaaggttag aaggcaagct tctaactttt tttgatatca
     5941 aaaatatcat gttaattcac tagtgttaat ttactttaat gagtctaagt ttgtatttta
     6001 tacagaatga gtaacgttta ttggtattca tcaaaaattg tttacttttt ttc
//
LOCUS       DAUCWU010000089         5998 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUCWU010000089 DAUCWU010000000
VERSION     DAUCWU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.089"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(251..1393)
                     /locus_tag="VGY24_001516"
     CDS             complement(251..1393)
                     /locus_tag="VGY24_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEQ4284483.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIVSNFAKKVFYQKTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1674..4652
                     /locus_tag="VGY24_001517"
     CDS             1674..4652
                     /locus_tag="VGY24_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ4284484.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEKPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRQ
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSRWYGFTGTPIFMENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLFASVQVYCDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4665..5873
                     /locus_tag="VGY24_001518"
     CDS             4665..5873
                     /locus_tag="VGY24_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ4284485.1"
                     /translation="MTKSKQPQYRFDSFEGEWEEKKLGEISNIVRGASPRPIQDPKWF
                     DAKSDIGWLRISDVTEQEGRITYLQQRISELGQEKTRVLKDPHLLLSIAATVGKPVIN
                     YVKTGVHDGFLVFLDPKFNREFMFQWLDMFRPYWNKYGQPGSQVNLNSEIVRNQVINL
                     PSLPEQGTIGELFQTVDQLIQLQRQKLATLKEQKQTFLRKMFPPQGQKVPEIRLQGFD
                     GEWEEKKLGEISRMFSGGTPNVGIPEYYNGNIPFIRSAEINSDQTELSITDKGLSNSS
                     AKLVEKNTLLYALYGATSGEVGLSRISGAINQAILAIIPEKKYSSLFIKNWLYKQKSS
                     IIEKYLQGGQGNLSGSIVKELTIHFPSLSEQEAIGDFFQTLDQQMSQTEDKLIELKAL
                     KQTLLNRLFV"
     gene            5886..>5998
                     /locus_tag="VGY24_001519"
     CDS             5886..>5998
                     /locus_tag="VGY24_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEQ4284486.1"
                     /translation="MAKKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIF"
BASE COUNT         1990 a         1186 c         1087 g         1735 t
ORIGIN      
        1 acaacttctt tattctatca ggtttctcat agcctgtcaa ctactttttc atcttttttc
       61 aataagtgtg ttacagcaac ctctctgaca gctttattag tatacgatat ctttactgtc
      121 attgtcaact ctttttttac aaaatgttgc tgtttttcat actatgctaa tgagttagct
      181 cgtgcgtctc cttcttcata ggttactttt aaccgccaaa tgaatgctac acaatcgaaa
      241 atagatgagt ttacttatta aactgagcta tcaatagcat aaggttagct gctaatattt
      301 tgatattaga aagtgttgtt gttttatcta tcgctgcttc taaggaaatt ggctgctgtt
      361 gtattgaaaa aacagccaaa aagtcttctg ctgctagtcc tgtctcaatc tttacagaac
      421 cacatagagc aatcgttgga acttgatatt ttttagccat tcttgcaatc gcaacaggta
      481 ccttgccaga ttgtgattgt gtatccaggc atccttctcc agtaataact aaatcacaag
      541 actgaaggga attatccaga tttagtagct ctgcaatcct gctaaaacca ctagttaagg
      601 tgcctcctaa taggacaatg gcacctccta atcctccagc tgctccactt cctggtattg
      661 tctgtaaatc aatggttttt tggtaaaaaa cttttttagc aaagttactt acaatttgat
      721 cagtttcttc aatttgacta agactcccac ctttttgagg gccaaagacc gcagcaaatc
      781 cttgagggcc atgatatgga ttggtcacat cagtcagccc taatagcgta acaggtgatg
      841 ctaaagtatc aagataagat cttcctgtca tgaaatcata gtttaaactt tccaaaaacc
      901 ctttgccacc atcagaagtg cctgttcctc ctagcatgat ttcgatttga gtagctcctt
      961 tttgaatggc atctttaact gccaaaccca aaccatagga agtggcttgt gcataggtta
     1021 ctgaattggg ggtgatctta tcaataccaa taatactcgc agattcaata aaagcttgct
     1081 ttgcgtgacg ataataagcg actttaatcg ggcgtcttaa tagatcaatg gtttttactt
     1141 gatgccacct ccccgccacg gtttgtgaga gggctgctaa acttccttct cctccatcag
     1201 cgatagctct tgtttcaata actaactgct tatcgacaga aagaagggct tgagctacac
     1261 tagtgttaag ttctggagac gttaccgatc ccttaaaaga atctatggct accaaaattt
     1321 tcatatcttc aataaattcc ttttctgtat attgtagcag attatcgtcc tttaaaaccc
     1381 tattaatagt caagctagat ctgtcttaag ttttttatct attttgagaa ctcgaccttt
     1441 ttactagtgt tgtcttatta ctttcttcac cttaaattat tgtcaagcaa actcaaaaat
     1501 acaagaactt tctcgattgc atttacatag tcatagcttt gtttagttgc taggatctta
     1561 agcattcaac taatcaaaaa aagactgttt ggaaggtttc aatagctttg ccattctaaa
     1621 aaggatccgc caatgatata atactactat atcatgccga atggggatta aaaatgggaa
     1681 atcaaactaa aacggaactt gaacttgaaa aagagttgat ccatctctta gaaacaggtg
     1741 aaagtcaatg gacatatcgt aaagaattaa aaactgaaga tgctttatgg gataatttct
     1801 ttaagatctt agcgcaaaat aacacgcaat atctgaatga gaagccttta acagctagtg
     1861 aaaaagagca gattaagaat caacttaact ttgttaatta ctatgaagcg gctaaatggt
     1921 tagctggtga aaacggcatt gctaaagtac aagtgcaacg cgaagatgca aaactaggaa
     1981 cgattcgttt agaagtcgtt aaagcagaca atgtggctgg tggaacttct gtttatgaga
     2041 ttgccaatca agttgctttc tctggttctc gtgatagacg aggtgatgtg acacttctca
     2101 tcaatggctt acctatgatc caaattgaat taaaatctca gaatcatcaa tgcattgaag
     2161 cttttaacca agtcaaaaag tatgataaag aaggccagtt tagaggtatc ttttcaacct
     2221 tacaaatgtt tgtggtctca aataaaacag atacaaggta tatcgctgca gctaaagaaa
     2281 ataaattaaa ccctaacttc ttgacccaat gggtagatca aaacaacaaa ccacaaaaag
     2341 atctctttgc ctttgccaaa gaagtcctat ctatccctcg tgctcaccaa atggtcatga
     2401 cttactctgt catcgatgat gataaaaaag cactaattct ccttcgtcct tatcagattc
     2461 atgcaatcga agccgtcgct gaagcgagtc gtcaccgcca gtctggctat atctggcaca
     2521 caacaggttc tggaaaaacc ttaacttcct ataaagtggc tcgcaatatc cttcaaatac
     2581 cggccgttga aaaatccatc tttgtcattg accgaaagga tttagataat cagaccactt
     2641 ctgcttttca atcttatgca caaaatgata tctttgatgt cgatgagaca gaagatacga
     2701 gacaactcat caaaaattta gaatccagtg atcgtcgtgt tgtggtcacc accattcaaa
     2761 aactcaatgc tatgatcagt cagatggaaa gttacgacac tccaaaattc aagaaactaa
     2821 aagagcgtct tgcacaccta aatgtcgtct ttgttgtaga cgaatgccac cgtgctgtca
     2881 cgccagaacg tcaacgtcat ctcacgaata cttttcgaaa cagtcgctgg tatggcttta
     2941 caggtactcc tatctttatg gaaaataagc gtgctcaatt aggtgattta gcccaaacaa
     3001 cggaacagca atacggcaaa tgtctccatc aatacactgt caaagaagcc attcacgata
     3061 aagcggttct aggcttccaa gttgagtata agacaaccat tcctgatatg cctgaagaca
     3121 gtattcctga agaagcttat gatcatgaag aacatatgtt agctgtctta aatagtatca
     3181 ttaatcaatc acgtaagaag cttggcttta ataatgggat tggtcagaca ttcgaaggct
     3241 tattaaccgt taagagtatt gctcgtgccc aagcctatta tgatctgatt cagaaagtta
     3301 aagctggtga aacggattta gtcatttcta agaaggtcaa agaaaaacta ccagactttc
     3361 caaaggtagc tattacttat tctatcactg aaaatgacaa tgcgtctatc agccgacaag
     3421 ataaaatgac caaaaatcta gaagactata atcacctctt tggcactaac tttaccatcg
     3481 ataacttaca aggctataac agagatttaa atgatcgctt agcccgtaaa aaagataaat
     3541 tcaaagaccg tcatgaacaa ttagacctcg tgattgtggt tgaccgtctc ttaacaggtt
     3601 ttgatgcgcc ttgtttatca acgatcttta ttgatcgcca gcctatgaaa cctcagcaca
     3661 ttattcaagc tttctcacgc accaaccgta tctttgaaag tagaaaacat tacggtcagg
     3721 ttgtgacttt ccaaacacca cttcgtttta aagaagctgt cgacaaagcg cttagcctct
     3781 actctaatgg gggtgaaaac gacgttttgg ccccttcttg ggaagaagaa aaagcaagat
     3841 tctttgaaaa agtaacagta ttaaaaaata tagttcctga tccagacgct ttcccaacta
     3901 ttgagagcgc acagacagct tttctcaaac aatacgccaa agcctttcaa gcgtttgata
     3961 aactctttgc atctgtacag gtctattgtg actttaatga aacactgttg agtgaggttg
     4021 ggttatcaga tgaagtgatt gatacttata aaggcactta ccaaaatgtc attgcagaaa
     4081 tacgaaaacg aagagaagat gacgaagcca tcccagagat caatatcgac tatgagctag
     4141 aaagtgttca gatggatgat atcaactatc actatatctt gaccttaatt caagcctttg
     4201 tcgatcaaga acaagaagct cttcaagaaa ggctcaatga caatcccatg gatcaataca
     4261 tccaagattt ggcaaaatca aaccctgcta tggctgactc actagctgag ttatggcaag
     4321 atatccaaaa agaacccaaa gcctatgaag ggaaaagcat tgcttatgag ttggataacc
     4381 tcattggtga taagatccaa agggccatca aacactttgc ggaccaatgg aaggcagatc
     4441 ctgacaaatt agcctttgtg gcgactaatt atcatagtgc taactctacc aaacaagtgg
     4501 gcatgtcaac cttaaaagaa tccctagact accaagccta taaagaaaaa caaggtgact
     4561 cggccatgaa taaactcaaa tacaagagcc aatttgaacg ggagttagtt caattcatta
     4621 gagatcaaat ccaaccctta aaggggcact aggaaaggaa agagatgaca aaatctaaac
     4681 agcctcaata tcgctttgac agttttgaag gggagtggga agagaaaaag ctgggagaaa
     4741 tttctaatat agttcgtggt gccagtccta gaccgataca agatccaaaa tggtttgatg
     4801 ccaaaagtga tataggttgg ttaagaattt ctgatgttac tgaacaagaa ggtcgtataa
     4861 cttatttaca acaaagaata tctgagcttg gtcaagaaaa gactagagta ttaaaggatc
     4921 cacatttact attaagtatt gcagctacag taggtaaacc agttatcaat tatgttaaaa
     4981 ctggagtaca cgatggattt cttgtgtttt tagatcctaa attcaaccgt gaatttatgt
     5041 ttcagtggtt agatatgttc agaccatatt ggaataaata tggtcaacca ggaagccaag
     5101 tgaatctgaa ttctgaaata gttcgaaatc aagttatcaa tctcccctct ctccccgaac
     5161 aaggaaccat tggcgaactc ttccaaacgg ttgatcagtt aattcagtta cagaggcaaa
     5221 agctagcaac ccttaaagaa caaaagcaaa ccttcctcag aaagatgttc ccacctcaag
     5281 gacaaaaagt ccctgaaatc cgtttacaag gttttgatgg tgagtgggaa gagaagaaat
     5341 tgggggagat atctcggatg ttttcaggtg gcactcctaa cgtaggtatc cctgaatact
     5401 ataatggtaa tattcctttc attagatctg ctgagatcaa cagtgatcaa acagaattat
     5461 ctatcacaga caaaggtcta tcaaattcat cagcaaaatt agtggaaaaa aatacattat
     5521 tatacgctct ctacggtgca actagtggag aggttggact ttcaagaata agtggagcta
     5581 ttaatcaagc aattttagcc attattcctg agaaaaaata ttcttctctt tttataaaaa
     5641 attggttata taaacaaaaa tcctcaatta ttgagaaata tttacaaggt ggccaaggaa
     5701 atttatctgg ttctattgtt aaagaattaa ctattcattt cccttctctc tccgaacaag
     5761 aagctatcgg tgacttcttc caaaccttag accaacaaat gagtcaaaca gaagataaat
     5821 taatagaact taaagcactc aaacaaacat tactcaatcg tttattcgta taagaaagga
     5881 cgcacatggc aaagaaaaca acttcactac ggcaagcact atggcattcg gccgatcaat
     5941 tacgtggcca aatggatgcc aatgactata agaactatct tcttggactc atctttta
//
LOCUS       DAUCWU010000090         5856 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUCWU010000090 DAUCWU010000000
VERSION     DAUCWU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.090"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..356
                     /locus_tag="VGY24_001520"
     CDS             <1..356
                     /locus_tag="VGY24_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ4284487.1"
                     /translation="EQVLAFLDISQTTKGHTLVIPKQHVRNLLEMTAETASHLFARIP
                     KIARAIQSATGATAMNIINNNEALAGQTVFHAHVHLVPRYNEEDGISIQYTTHEPDFP
                     VLEKLARQINQEVSS"
     gene            353..709
                     /locus_tag="VGY24_001521"
     CDS             353..709
                     /locus_tag="VGY24_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ4284488.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLSEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(820..2094)
                     /locus_tag="VGY24_001522"
     CDS             complement(820..2094)
                     /locus_tag="VGY24_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ4284489.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGQSASTDQDSTASSHTPT
                     TPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(2103..2627)
                     /locus_tag="VGY24_001523"
     CDS             complement(2103..2627)
                     /locus_tag="VGY24_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ4284490.1"
                     /translation="MVQQSLVIEEGVPQDAKAVLSLVTDAAKETDFITGVETILEATP
                     QELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            complement(2602..3063)
                     /gene="tsaE"
                     /locus_tag="VGY24_001524"
     CDS             complement(2602..3063)
                     /gene="tsaE"
                     /locus_tag="VGY24_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ4284491.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHGSAKSRY"
     gene            complement(3217..4677)
                     /locus_tag="VGY24_001525"
     CDS             complement(3217..4677)
                     /locus_tag="VGY24_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ4284492.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSGVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGVTFLFIVNFIILTIL"
     gene            4947..5759
                     /locus_tag="VGY24_001526"
     CDS             4947..5759
                     /locus_tag="VGY24_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ4284493.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLTQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
BASE COUNT         1962 a         1210 c          972 g         1712 t
ORIGIN      
        1 atgagcaggt cctcgccttt ttagatatct cacaaactac aaaagggcat actctagtca
       61 ttcctaagca acatgttcgt aacttactag agatgactgc tgagacagct agtcatctct
      121 ttgctcgtat tcctaaaatt gctcgtgcta tccagtcagc tactggcgct actgccatga
      181 atatcattaa caataacgaa gccttagcgg gacaaactgt tttccatgct catgttcatc
      241 tcgttcctcg ttataatgaa gaagacggta tctcaattca atacaccact catgagcctg
      301 actttcctgt cttggaaaag ctcgcccgac aaatcaatca ggaggttagt tcatgaaagt
      361 taaaaccgtg atagccgtcg gattattatc ttttaccgct tacaaagcat atcaaaaacg
      421 ttgtaccata aaagagctgt tatccatcag tagacaagca aaagatgctg cccaacttga
      481 tttagataac attaaagcaa accttgacct cattcacagc caaggaaaag taattcaaaa
      541 tattagtcag gacttggccc ataaatggcg ctactttaac caagagacac aagcacacct
      601 ctctgaaatc caaaatcgta tggccaaata tcaagaagat agtgagctaa tttctagtga
      661 atcacctact tgccaaaccg tcattttaac taacaaccat tccagatagt attatctccc
      721 tcatatactt tatttgaaat cacagacagc tatcatcttt gatacctgtc ttttttatat
      781 tcaaaatcgg atattttaaa cataaaaacc ctcagaacgt taggtctgag ggttgatatt
      841 agcaggagga actaatgatc cagaccctga tgagctagac tcatcagctg ctaaagcatc
      901 tgatgaagaa gacggcgtag ttggcgtatg gcttgaagca gtagagtcct ggtctgtact
      961 agcactctgg ccggtatcta ctccacttga ataattagcg taagagctat gactatcaga
     1021 ataagatgga gcctggcctg aagagtcata gttgttgttt actgtctgag acttagttga
     1081 cccatacaaa ttttcataaa cagtagcatt tgtttttaat tgactaacct tatgaagtcc
     1141 taattctgtt cggatacgat tttggatttc taacaaatga ttagaggtaa caatttggta
     1201 tgatccacca tctgataaag tggcatcttc tccttttagt tgataagtct taatagttct
     1261 aagtgcgtca cgataaccta atagactagg gatagtgcga gaagagattt cgatattcgt
     1321 ttgcatatta ctacttacag cagataaaat cttccgataa gagctaatgc tatcaagagc
     1381 aaggattttt ttcaatacct tttgaatgac ttcacgttga cgcttttgtc gaccataatc
     1441 tccctcagga tcatcataac gcatacgagc ataaaccaaa gcttgttcac cgttaatttt
     1501 gtgtgttcca ggcgcaacag tagcttgata ttcaggttcg ttttcagcaa tcgagatagg
     1561 aaaatcaaac tcatttgtaa ctgtaatccc tccaactgca ttcacaagat caataaggcc
     1621 ttgcatatta atttgaacat agttatcaat ggtgatattc aaaagatctt gcacggtcat
     1681 aatagccatc tgagcgccac ctgctgcata agcagcgtta agcttagctt caacaccatt
     1741 catttcatta tttttgggtc cagataacgt ggttaaggta tctcgttcta aactagtcat
     1801 agttgttttc ttggtctttg gattaaccgt aaccaaaatc atcgaatcac tgtttccttc
     1861 ccacttggag gcacgctctg aagagcctgt atcaacaccc atcaacaaga tagaaaaagc
     1921 tcttgtttgt ttaatggcat cacttttctt tgaagatgtc gaaaaatctt taaaggtctt
     1981 tgataattct cctgttgaga aggtataagc actagttaga tagacaccta atgccaagac
     2041 agttgttaac acaatagctg tgaacattaa aactattttt tttccaattt tcatcgattc
     2101 acttactgac ttacagattt acgcatatga taaacatcta agtcatctcc attttttgat
     2161 ttgatgtcgt tttttcgcat actttcaata cgaaaaccat actttttata caggtaaata
     2221 gccttagtat ttcttacttg aacgtccaac ttaaggcttt cgatataagg gttttcttca
     2281 gcccaatcaa gagcaatctc taacaataat tgaccgatac cataaccacg gtaagtctta
     2341 gcaactaaca taaatacatc tgcttcagct tggccttggg ataatacttc cccagaaaga
     2401 tttaaaagcc ctactacttt ttcatctaat ctagctagca ggcaaaaatc aataaaagag
     2461 gtttgacttc ttgataaaaa gtctgacaat tcttgaggag tagcctctaa tatagtctct
     2521 acgcctgtta taaaatctgt ttctttggca gcgtctgtca ctaaggaaag tacagctttt
     2581 gcatcttgag gtacgccttc ttcaataacg agactttgct gaaccatgtg acattatctc
     2641 cagaagctga cgagagcgct caccatgcgc taacaagtct agttgacgac ctttgtctcg
     2701 tttagtaatg gtaatttgca aatagtcttg taataatccc tctcctaata attctcccca
     2761 ctcaataacg gtaacaccat taccaaaaag aaaatcatct aaatcaatag agtctggatc
     2821 atcaccaaca cggtaaatat ctaagtgata aagaggtaga cgtccttcat attctctaac
     2881 aatagtataa gttgggcttt tgatcatttg actaatcccc atccctttag caataccttt
     2941 tgccaatgtt gttttaccgg cacctaagtc gccagacaat acaatcacat ctccaataga
     3001 caaataagta cctagggttt ctccgtaagc tttaagagta tattcatttt cactataaaa
     3061 catagcaaca ttatatcaga aaaaaaacat acttccaagc actacctaga agatgactcc
     3121 aataaggtat tcgtaaagta aaaacgcttc tttatattaa aggttaaaat caattaaaga
     3181 agcggcagtt agtagcaaaa taagtaaatt atttggttat aagatagtta atatgatgaa
     3241 atttacaatg aacaagaagg ttactcccca aataataggg tgaatatctt ttgtttttcc
     3301 ctcaacaact tttactagac aatagaaaat aaaggcagcg gcaataccat aggaaataga
     3361 gtaacacagc gccataaaga aagctgcaaa aaaagctgga agagcatctg caaatttact
     3421 ccaattaaca tcaagaaaag aagacaccat catgacaccc acaacaatta aagctggagc
     3481 cgtagcagca gctgggacaa tacctacaag cggtaatagc aatattgata ataagaagca
     3541 tactgcggtg gagactgctg ttagtccagt acgtccacct tcagcaattc ctgctgctga
     3601 ttcaacatag gtagtcgtat ttgaagttcc aaccaaagcc ccaatagaag taccgatagc
     3661 atctgcaaaa agcgcacgat ccatttttga actaaagcct atactatttt ccaaagcatt
     3721 ctcatcgtct tgagagaaaa tacctgttcg acgaccagtt ccgataaagg taccaattgt
     3781 gtcaaaagta tctgatagac taaaagcaaa aatagtcatg aaaactagcg gtaaacgact
     3841 tgaatcgcta aaaagagaac tcaaaccatc gaaagctgca aggaaagttg tacctaattc
     3901 agtccaagct tcaccaatat ggctgatccc aaaattgagg ttggacacat ccactattcc
     3961 taaaggaata cctacaagag ttgttgcgac aataccaatt aaaatagcac ctctaaaatt
     4021 tcgaatcaca agagctgtcg ttaaaagtaa accaaaaata gcaagtaata caccggaatc
     4081 cgtaaaacta gaaatcgtag gtacaactcc accattggcg tctacaaata atagaccatc
     4141 tgcaaatgtt ttagccgatg ctttagccgg ttcaacacca tttaccatta ctatattttc
     4201 agcagagata gaaaaagtaa tgatatttgc gtttttaaat cctaaataag ctacaaagac
     4261 accaattcct ccaccaatag catgctgtaa actaactgga atcgccttga tgatactttt
     4321 acgaaacttg gttacggtaa taaaaatatt gaataatcca caaatgaaaa ccattgccaa
     4381 ggcttcttgc catgaaaacc ctaaagcaaa aacaactgta taagtgaaaa aagcgttaag
     4441 tcccattcct ggtgccagcg cataaggcac attggcaaat aatcccataa ttaaggttga
     4501 tatggctgct gcgataatcg tagccaaaaa gacagcatta gagggcatcc ccgctgcacc
     4561 taaaatactt gggttaacaa acaaaatata ggacatggca aaaaaggtcg ttaaaccagc
     4621 cataatctca gttgagacag ttgtcccatt ttcgcttaac ttaaaaaact tttccattga
     4681 aatggagtct ccttaattaa atagtaggta aaatcaaaaa gcgcttatca ctagataagt
     4741 ctttttgaca cctcattatt attgttattg ttattgttcg tattaaaatg aacaataaca
     4801 atatgttacc atttttttgt tcacttttca acgtctataa cttcatttat tcattaaaaa
     4861 aacagttatc ctatcttttt tactaatgaa tacgcttaac ttatcttttc tctttatctt
     4921 ggagcaaaat ttgctataat ttaattatga ctaaaaaatt gattgccatt gatttggatg
     4981 gaaccttatt acatcatgac aacaccatct ctacctatac tcaaaaaacc atcaaagctg
     5041 tccaagacaa gggccaccac gtcattattt caacaggccg tccttaccgc atggcactgg
     5101 actactatct gcagctcaat ttaaagaccc caatcattac ttttaatggg gccttgaccc
     5161 acatgcctga acaaaaatgg gcctatgaac ataatgtcac tttggacaaa ggctacctac
     5221 taaggttact aaaatatcag gatgatttcc agatggattt tattgccagt gagtatcgaa
     5281 aaaatgtcta catcaccatg accaatccag aaagtatcga cccgcaactc tttggtgttg
     5341 atgaaattac tcaggacatg gcattagaga ttaccaaaat cactcgaaat ccaaacgcct
     5401 tattgacgca aacgcatcac gaagacaagt atgccttagc taaaaacatg cgtgctttct
     5461 tcaaagatga aattgaaatt gattcttggg gaggccctct caacattctt gaaatttcgt
     5521 ctaagaatgt caataaagct tacgctctga actatctcct tggaatttac aatatggata
     5581 aaaaggattt aatcgccttt ggtgatgaac acaatgacac tgagatgcta gcttttgctg
     5641 gaactggtta tgctatgaaa aatgctagcc ctgtgctact gccttatgct gatcagcagc
     5701 ttaatttttc caacgaagaa gacggtgttg ccaaaaaatt ggaagaactt ttcttgtagt
     5761 agcaaacgaa accactattt ttcagaaaat ggtggttttt cttctgtcaa cagctatcaa
     5821 aaaatgaaaa tcattttaat aatttcaaca tttttc
//
LOCUS       DAUCWU010000091         5824 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUCWU010000091 DAUCWU010000000
VERSION     DAUCWU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.091"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(87..1424)
                     /locus_tag="VGY24_001527"
     CDS             complement(87..1424)
                     /locus_tag="VGY24_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4284494.1"
                     /translation="MEEDYNKREPEKFTQFLCRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            complement(1756..2715)
                     /locus_tag="VGY24_001528"
     CDS             complement(1756..2715)
                     /locus_tag="VGY24_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ4284495.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLTTAKDLA
                     KAYHMPLATSKYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQVQLSLHGHEAQVVNHNKH
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSLAVMAVLERAVHS"
     gene            complement(2791..3489)
                     /locus_tag="VGY24_001529"
     CDS             complement(2791..3489)
                     /locus_tag="VGY24_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ4284496.1"
                     /translation="MTKVVLVTGCASGIGYAQAHYFLKQGYHVYGVDKSDKPDLSGNF
                     HFIKLDLSSKLSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            complement(3482..3718)
                     /locus_tag="VGY24_001530"
     CDS             complement(3482..3718)
                     /locus_tag="VGY24_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ4284497.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            4079..4810
                     /locus_tag="VGY24_001531"
     CDS             4079..4810
                     /locus_tag="VGY24_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284498.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDAIKWELDPDTSPAIHVTVSP
                     ENGNNKLQFTLPRGWSVSFLKKKNHNSEPYSPLKINSESLDEYAKRENNNEIYGKKNT
                     SPKVITKPSTKGNESSNHSNTNLQLSFIYNDNHGDGGLPITVTVDKPNEGDELTLLYR
                     ADDGFFGGFTKLKDSKTLKKEKEKKERQLAEEKQIEEERKHVEQKQLEESMLKQIREE
                     DHKPWHQRLSESIQDQWWNFKGLFQ"
     gene            complement(4905..5156)
                     /locus_tag="VGY24_001532"
     CDS             complement(4905..5156)
                     /locus_tag="VGY24_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284499.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     SLVQNKDRQKVWSLAGLVLAAIAVAVVAYAYYDPTVIPY"
     gene            complement(5322..>5824)
                     /locus_tag="VGY24_001533"
     CDS             complement(5322..>5824)
                     /locus_tag="VGY24_001533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284500.1"
                     /translation="EQPVNAEGVPIKGVQQAEKNAENITYKEYMAQFGLGELEEPYYK
                     SRFQGYLDGYKEGLEGSRSTSPEPKDIQLPNGFSGDPVGYTDGYEEGFGKGAHKHRPL
                     EAEDASPDTEGQTGGPSSRESATHDEKNQTDESPGGEDATDGKEISDIIFEIITEVIS
                     WFYNLF"
BASE COUNT         1849 a         1335 c         1075 g         1565 t
ORIGIN      
        1 tcttttatag tgtcatttgc ggcttgtgat gactaaaaaa gacaggactt cagcaataag
       61 tctgtctttc ttaggcattt gcctagctaa tctttgatga tttcttcctc acgtttggct
      121 aaaatcaaat aaccaaccgc tgaaaaagca gataaggctg caacgacaat atatgaccaa
      181 aaccagccaa aaccatctgc caaaatcggc atcagccagt ttgctcccat attgccgatg
      241 agataagctg ttactcctac aaaaccaatc gctgttcctg caacatttaa aggaacaaag
      301 ttaattgtta aaatattcac gatcaactgt gggccataaa tcaatgaccc taaaatacct
      361 gcaataacaa gcaacaagaa atagtttgga gcacctgtag cagtcaagcg ttcatagaca
      421 aacacaatag ccgccaacac aaaaagtcca atagccccaa tcttggccat tttattagga
      481 taacgaaccg ctaaccacgc aaataccagc gagcctggaa tagctaccca ctctaacatt
      541 gaaatcgcaa aatgaatatg ggcctctgac atattggcta cttgtgacaa gtagatcggc
      601 atccagtctt caatcccaaa acgaacaaaa taaagggcca cattgacagc agcaacaagt
      661 aagagcgacg ggttacaaaa aacgtatttc cagatgagtt gccaataaga caggttatgc
      721 ttttcttctt cactggcaac agctgattcg cccgcatcac catacatcgt tcgaagagag
      781 tcaagtccct cagactcagg gttatcaccg ccaagcttcc agcaacacaa agcaaacaag
      841 aacactaaaa cgccagggat taaaaaggca agtaaaatat tgccattagc tggtaccacc
      901 aagcccatac tggtgagcaa ggcgatggtt aatggcaaga gggctgatcc catgttttga
      961 gaaatgttcc atccagcgat ggctccgccg cgtgttttat ttggaaaata attggcaatc
     1021 atggcttgtg aggcaggtgc caaggcacct tgtacgacac cacacaagac tagcagtatt
     1081 cctaatacca ccattgaact attgaaaaaa ccgattaaaa tacaaatcaa agcgcttgca
     1141 cccaagctga tcgaaaagag cttcctcaag ctaacacgat cccctaaggc tcccatatag
     1201 aattttgcta agccataaga aacggtcaaa cacgataaca aggtcgcaat ttgagccttg
     1261 tcccaaccat tttgcaccat aatagtgttt gacattagtt tgaaattgtt gcgtaccaag
     1321 taggcacaca cataaccaaa aaatgcgaca aaaaagacga ccttttgacg gcacagaaat
     1381 tgcgtaaact tctcaggttc tcttttgtta taatcctctt ccataaacaa ttaaactcct
     1441 acactgtaat ttttgtcatg acattctctt agtgtaacag agttatgcag tagtgtcaac
     1501 gcttacaaat tcgattttac tgcttctaga gcccctttgg gcagaatttc cgaacggtca
     1561 tttcttaaat cgtttggctt tatcattatt ttaatcaaat caattcgtca ggcatctctc
     1621 atctcacaaa gaaggcccaa atcctgaaca agttattttt accaaaaaag cacgaggggg
     1681 ttaaccctcg tgaaaacacg tctttcaacc attaccatag cacctgcaca aaaggcaatg
     1741 gctaccagat tacaatcacg aatgaacagc tcgctccaac actgccataa cagctaggct
     1801 atgttccaaa gcctgattca ctttctcaaa gtctctttgg tcaatcatgt ccctaaaagc
     1861 gacaaactct tcatacatcc ggtggtcatg cttgttgtga ttaaccactt gtgcttcgtg
     1921 accgtgtaga cttaactgaa cttgaggaag ggtatttgtt gctccaagca ctgccagtga
     1981 ccctttatta ccttggatgg ttgatttgat ttctgcagtg cagtctttgg ccccgataca
     2041 caccacttta aactgctcat agtccatgac aagcatgcct gaggtgtcaa tgcctttttc
     2101 gacattggct aaatattgaa ccgttttagg gcgaccaaat aatcctacca caaaatgaat
     2161 gttgtagatg tttaaatcac gtaaagctcc accacccatt ttaggattaa aagctggcgc
     2221 gatatcacct cttttgaagg catcgtagcg tgaagaatat tgtgaataat tgcattctac
     2281 aattttgata tcgccaagtt ggtcaagatg ctctttgata aaggtcatgt tgcttaaata
     2341 ctgattggta atcgcttcta gcaaaatgag ttttcgtttt ttagcgatca ccactagttc
     2401 atctaattcc cctgctgtca tggtaaaggg cttttcacaa atgacatgtt taccagctag
     2461 aagggcctcc ttagcatagg caaaatgcag gtgattcggc aatccaatat agaccgtgtc
     2521 aatctcctca ttgcctaaga cagcctcata tttactcgta gctagaggca tgtgatatgc
     2581 cttagcgagg tctttagctg tcgttaaact cctaactgtt gataagatag caactaaatc
     2641 aataccatca attttctgca agacaggcaa aacttcctta acaatcatac ctgttcccaa
     2701 aaccgctaat ttcatttgtt catctccttt gactgtttga ctcgtcacac ctctagggct
     2761 tactgcgcca gctcaagcta aggcatgtga tcatttcaaa gtccagccac cgtcaatttt
     2821 gacaatctcg ccttgcattg atctcgcttt tcctgacgca agaaatcccg ttaattcagc
     2881 aacttcatct ggttcagtcc aacgcccaat tggcgtctca cgagctaccc attctgctaa
     2941 gccaccaggc tcaaagtcgc tagccgtcat agcggttttg acagcacctg gtgctatacc
     3001 aaagatgtga atctgatctt tggcgtaatc caaagccagc tggcgcgtga agcccgcaag
     3061 cgcatgctta gaactcgtat aggcaacacc accgccaccc gctataaaac ttgctattga
     3121 gcacatattg atgataacac ctgactgctt ttccaccata cgtctaagat aatgacgtgt
     3181 taacttcacc gtcgcaaaaa aattaatatc aaataggtgt tccacttctt catcactgac
     3241 gtctaaaagt ggcttgtagg catctaaaat ccctgcagta ttacacaaaa tatcaacact
     3301 ggggaccact ttaaatagtg gggataactt agaggataaa tccaatttga taaagtgaaa
     3361 gttaccgctt aagtccggtt tatccgattt atccacacca tatacgtgat acccttgttt
     3421 caaaaaatag tgagcctgtg cataaccaat cccactagca cagcccgtca ctaaaacaac
     3481 tttagtcatt gacctcgatc caatcctctg ctaacacatc acatggtgtc ggcgcccaca
     3541 tggaaaaacc ttcacctgca ccagtaacat gaatcagaaa ataaggtgtc gcttgtagta
     3601 ctttcccatc cacttcaagg gtatcaaaca actgcacata attttcagcg ccaccccaac
     3661 ctgtacgtac atattttttc ttggctttta aacctggtaa aatctcttca aatgtcatat
     3721 tgttctcctt tattgacgaa atagacactt taggcagtct ataaactcct aaaaataaat
     3781 ttgtggactc acgcatcatt catcgaaaat gctatcttct tttatttagt ctatcataac
     3841 gcatttttgt caaaaagcaa ctaaaagtat tggcaacctc aaaacacgac ctgatgccca
     3901 aacggcttgc aaaggtaaaa gggcaacggc tagcaactgg ctaggaaaaa aactgtctgc
     3961 ccctaccaag aatagtcgcg ccatttccca accacaagac ataaacgccc tatcttttta
     4021 gcgcaaaaat tggtataata agccttgtct taaaaaatga gcaaaaggag aaaaaaaaat
     4081 gaaaaagaca ttaaccttgc tactggcact ctttgccatc ggggtaacca gtagcgtcag
     4141 agcggaggat gcaatcaagt gggaactaga tccagatacg tcgccagcta ttcacgttac
     4201 tgttagccct gaaaatggta ataataagct acagtttaca cttcctagag gctggtcggt
     4261 atctttttta aaaaagaaga accataacag tgaaccatat agtccattaa agataaacag
     4321 tgaatctctt gatgaatatg ccaaacgaga aaataataat gaaatatatg gaaaaaaaaa
     4381 tacaagtcct aaagtaataa ctaaaccatc aactaagggg aacgaaagtt ctaatcatag
     4441 taataccaat ttacagctgt cttttatata taatgacaat catggagacg gtggtttacc
     4501 aatcactgta actgtcgata aaccaaatga aggagacgaa ttaacactgc tttatcgtgc
     4561 cgatgatggt ttttttggcg gatttacaaa attaaaagat tcgaagacac ttaaaaaaga
     4621 aaaagagaaa aaagaaagac agctagcaga agaaaaacaa attgaggaag agagaaagca
     4681 cgttgagcaa aaacaactag aagaaagcat gctaaagcag ataagagaag aagaccataa
     4741 accttggcat cagcggttaa gtgagagcat ccaagatcag tggtggaact ttaagggact
     4801 gtttcagtga gggtttacta gactatgagg ttggctaaag cacaaaaagc acctgtcagg
     4861 ggtgcttttt gtgttgctga ttgcgtgcga aggggaggtt aaggttagta ggggatgacg
     4921 gttgggtcat agtaggcata ggccactact gccacggcta tagcagctaa gacgagccca
     4981 gctagcgacc acaccttttg cctgtctttg ttctgcacca gactgataag ggctagacta
     5041 cttgctacaa gcggcaagta gagataacta taagtagcaa aaaagaagat atgatcaagc
     5101 aggcagacaa aactcaccac catgcttagg acgggcaaca ctttcttttc tggcatatat
     5161 gctcctttct caacacacgg gtcactaaaa ctcaaaaagg ccacccctat cagaggtggc
     5221 ctgactacta aagggactat cccaattata gcagattagt gtgggtaagg tagaacaaaa
     5281 gctactagca cgaagttgct gacagacaat ttattttacc tttaaaataa attgtaaaac
     5341 cagctaataa cttcagtgat gatctcaaaa ataatatcgc ttatttcttt accatcagta
     5401 gcatcttctc cgccagggga ctcgtctgtc tgattttttt catcatgagt agcactttct
     5461 ctgcttgaag gcccacctgt ctgaccttct gtatccggag aagcatcttc tgcttctaat
     5521 ggacgatgtt tatgtgctcc ttttccaaat ccttcctcat aaccatctgt atagccgact
     5581 gggtcaccgc taaaaccatt aggaagctgg atatctttcg gttcggggct agtagatcta
     5641 gatccttcca agccttcttt atatccatct aaataccctt gaaatctact tttatagtac
     5701 ggttcttcca attcacctaa cccgaactga gccatatatt ctttatatgt aatattttct
     5761 gcattttttt cagcttgttg cactcctttt attggtactc cctccgcatt tacaggctgt
     5821 tcca
//
LOCUS       DAUCWU010000092         5345 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUCWU010000092 DAUCWU010000000
VERSION     DAUCWU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.092"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..112
                     /locus_tag="VGY24_001534"
     CDS             <1..112
                     /locus_tag="VGY24_001534"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEQ4284501.1"
                     /translation="KEFLSMLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            159..1157
                     /gene="ruvB"
                     /locus_tag="VGY24_001535"
     CDS             159..1157
                     /gene="ruvB"
                     /locus_tag="VGY24_001535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ4284502.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITSQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1295..1732
                     /locus_tag="VGY24_001536"
     CDS             1295..1732
                     /locus_tag="VGY24_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ4284503.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREWGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            1755..2156
                     /locus_tag="VGY24_001537"
     CDS             1755..2156
                     /locus_tag="VGY24_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284504.1"
                     /translation="MIDMQDIVKKWSITRAKLELVSVIVILVCAVSVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2153..3928
                     /locus_tag="VGY24_001538"
     CDS             2153..3928
                     /locus_tag="VGY24_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ4284505.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGKKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
     gene            4237..>5345
                     /locus_tag="VGY24_001539"
     CDS             4237..>5345
                     /locus_tag="VGY24_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEQ4284506.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNPNIVGKPATWIAAQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGV"
BASE COUNT         1673 a          855 c         1087 g         1730 t
ORIGIN      
        1 taaggagttt ttatccatgt taaaaaagta taagtactat tttatattcg cagctctact
       61 atcttttaaa gtagttcaag agcttagtgc tgttgactgg tggcgattat aataaaagta
      121 ggccactgct ctcttttact ttatgttata atagtcctat ggctagaatt ttagacaata
      181 atgtaatggg aaatgaggag ttttcggatc ggactcttcg ccctcaatat ctacacgaat
      241 atattggaca agataaagtt aaggaacaat tcgctatttt tatagaagca gctaaaagac
      301 gtgacgaatc tttagaccat gttttattat ttggacctcc aggactggga aaaacaacaa
      361 tggcatttgt gattgccaat gaattgggag ttaatttaaa gcaaacttca ggtcctgccg
      421 ttgagaaagc tggagattta gtagctattt taaatgaatt agaaccaggg gatattttgt
      481 ttattgatga aattcaccgc atgccaatgt ctgtcgagga agtactatat agtgctatgg
      541 aagacttcta tattgatatt atgattggcg ctggagatac tagtcgtagt attcatttgg
      601 atctgcctcc atttacattg attggtgcta ctacacgtgc aggcatgtta tctaatcccc
      661 tcagagctcg ttttggtatc actggtcaca tggaatacta tcaagaaaag gatttgacag
      721 agattgtgga gcgtacagct actatttttg aaataaaaat agaccatgaa gctgcacgga
      781 aattagcttg tcgcagtaga ggaacgcccc gcattgctaa tcgattacta aaacgagtgc
      841 gtgactatgc tcaaattatt ggtgatggca tcataacgtc tcaaataact gatagagctt
      901 tgactatgtt agatgttgac cgcgaaggtc tcgattacat tgatcagaaa attttacgaa
      961 cgatgattga gatgtatcag ggaggaccag ttggtttggg gacattatct gtgaatattg
     1021 ccgaggagcg taatacagtt gaagagatgt atgaacccta cttaattcaa aaaggctttc
     1081 tcatgcgaac acgaacaggt cgtgtagcga cgcaaaaggc ttatcgtcat cttggctatc
     1141 cctatcaaaa cacttaatga tttttcaaat ctaagaaaat taatctgaac ctgctttttg
     1201 tgttcggatt ttttgataag ttgacagaaa attattagat tgatttctcc gatatcagtt
     1261 agaaatttga tataataatt caaaggagta tttaatgaaa aaagtatgtt ttgtctgtct
     1321 gggtaatatt tgtcgaagcc ccatggctga gtttgtaatg aagtcaatag tatcaagtga
     1381 tgttatgatg atagaaagtc gtgctacttc tgattgggag catggaaacc ctattcactc
     1441 aggtacacag tccatattaa aaacttacca aattaattat gatataacta aatgctccaa
     1501 gcagataact ataactgatt ttaacacctt tgactatatt attggtatgg atagcgataa
     1561 tgtgaaaaat ctcaaagaga tgtcgcaaca tcaatgggac tctaaaatct acctgtttag
     1621 agaatggggt gttcctgatc cttggtacac taacgatttt gaagaaacct accaattagt
     1681 tagaaaagga tgccaggatt ggctttctcg tttaatgagt aaagagtatt gatattagaa
     1741 gttgagtaaa agaaatgata gatatgcaag atatagtaaa aaaatggagt attacacggg
     1801 cgaagttgga acttgtctcg gtgattgtca tattggtttg tgcagtttct gtttttagcg
     1861 taagaatttc gaataagaca agtttgacct atgataaagg acgtatgcac tatactggtt
     1921 acgtgattaa ccataaaatg aatggagaag ggaagcttgt ctaccccaac ggtgatatct
     1981 atgagggtac ttttaagaat ggcctatttg aaggaaaagg aacttttact gctaagacgg
     2041 gctggttata taacggggaa tttcataaag gacaagcaaa tggcaaaggt gtgttaaaag
     2101 caaagaataa taaagtctat aaagggattt ttaaacaggg gatatttcaa aaatgagaat
     2161 aaaatggttt tcgtttgtta gggtaacagg acttttactg gttttactct accacttttt
     2221 taaaaatgta tttccaggag gttttattgg tgttgacatt ttcttcacct tttcaggata
     2281 tctaatcaca gccctcttga ttgatgaata cacaaaaaaa gagtctatcg atattatcgg
     2341 ttttttaaaa agacgctttt atcgaattgt tcctcctttg gtgttaatga ttttattgac
     2401 cattcctttt acgtttttaa ttaagaagga tttcattgct aatataggaa gtcagattac
     2461 agctgtttta ggatttacca ctaatattta tgaaatattg acgggaagta gttatgaaag
     2521 ccaatttatt ccacatctct ttgttcatac gtggagctta gctattgaag ttcattttta
     2581 cttgttgtgg ggtgtatttg tttggttatt agctagacgc aaggagactc agaaacaact
     2641 gagaggacta ctgtttttga tttctttggg aatatttgct attagtttct tgagcatgtt
     2701 tataagatct tttatgacct caaatttttc cctgatttat ttttcgagtc tttctcacag
     2761 ttttcccttc tttttagggg cgatgtttgc cacaataaca ggtattaacg agactactgt
     2821 tcgatttcaa aaaaatgtac gtttatggcc gcgccagtat gtccttgctg ctatgattgg
     2881 agcatttaca cttttgcttg tgctaacggt cacattggat tttaaccata tcacgaccta
     2941 tctatttggc tttgctttgg ctagcttatt cgcatcaatt atgatttatg cagcgcgtgt
     3001 tcttcatgag caaacaccag atgtacaaga gcctaaagct atcacttata tagctgatat
     3061 tagctatggg atttacctct tccattggcc attttacatt atttttagcc aattaatgtc
     3121 tcatattttg gcagttattc taacagtttt cttttcaatc ttatttgcca ctgtgtctta
     3181 ctatattgtg gaacctttgg tccaagggaa aaaaccaaac ctgcttggtt tagaaattga
     3241 ctgttctcct tactataagt ggattgtagg agggtctctc gccttagcat tattaacttt
     3301 aggaacttgt atgatagcgc ctaaagttgg taagtttgag aaacaattgt tggttagttc
     3361 cctacaacag gctcaaagta atatggagcg gacacataca ctagctgcag gagacgctaa
     3421 tgctttgagt gacgtaggta ttattggtga ttctgtagca ttacgctcta gtgcagcatt
     3481 ctcaaaaatt atgccacaag cgcaactgga tgcagcggtc agtcggaatt ttaaagaagc
     3541 tttcgattta tttaataacc aaattaaatc taagagtcta tctaagactg ttgtcttagc
     3601 agtaggtgtt aactcgcttg acaactattc tcaagcagtt cagtctttta ttgaggcatt
     3661 gccaaaaggt catcgtttag ttttggtttc tccatataat gccaaaaatg ccagtcaggt
     3721 tgcagaagct agagactatg gtcttaaatt gtccaaaaaa tacaagtatg tcactattgc
     3781 agactggtat aaagttgctg ttgaacatcc agatatttgg tatggcagtg acggtgttca
     3841 ttatagtgaa gatagtcaag gagcagaact atatgtctcc acaatccaga ctgctgttga
     3901 gaagtcagct aaaaaaccag ctaaataaag ttaaaaaatg tctaagagat aagtcagatc
     3961 tattgtgctc tggctttttc ttgtgaacaa atgatgatgg taaaatagta gtactttgag
     4021 gtttttaaaa gaaagcggtt tcagaaataa aacggctttt tttagatata tttctgaaaa
     4081 aaagttaatg aaaaatattg ataccatagg atttcagaaa taatttgaaa aaaacttcac
     4141 aaatttattg tgaaatgatt tacaaaattt caaaaagtgc tataataatt ttgtaaacaa
     4201 aattgtgaaa cttttaacaa tataaaggag tctcaaatga ctgaaggaca taatactgtt
     4261 gaaacaacct ctgtttctgt gacaattgat gctcttgttc aaaaaggttt agctgctctt
     4321 gaagagatgc gtaagcttga ccaagaacaa gttgactata tcgtagcaaa agcttctgtt
     4381 gctgccttgg atgcacacgg tgaacttgct aaacatgctt acgaagaaac aggtcgtggt
     4441 gtctttgaag ataaagcaac aaaaaatcta tttgcttgtg agcatgtggt taataacatg
     4501 cgccatcaaa aaacagtagg aattattgaa gaggatgacg tgacaggttt gacgttgatt
     4561 gctgagccgg tgggtgttat ctgtgggatt acaccaacaa ctaacccaac ttcaacagct
     4621 atctttaaat ctttgatctc gttaaaaaca cgtaatccaa ttatttttgc ctttcaccca
     4681 tcagcacaag aatcatcagc acatgccgct cgtattgttc gtgatgctgc tattgccgct
     4741 ggtgctcctg aaaattgtgt gcagtgggtt gaaacaccat cacttgaagc aacaaatgct
     4801 ttgatgaatc atgacggcat tgccactatc cttgcgacag gtggaaatgc gatggttaaa
     4861 gcagcttact catgtggaaa acctgctctt ggggtaggtg caggtaatgt tccagcctat
     4921 gtggaaaaat cagctaatat ccgtcaagcc gctcatgata ttgtgatgtc taaatcattt
     4981 gataatggta tggtctgtgc ctctgaacaa gctgttatca tcgataaaga aatctatgac
     5041 gattttgtag cagaattcaa atcttaccat acttattttg taaataaaaa agaaaaagcc
     5101 cttcttgaag aattctgttt tggtgtaaaa gcaaatagca aaaactgtgc tggtgcaaaa
     5161 ttaaatccaa atattgtagg aaaaccagct acttggattg cagcacaagc tggatttact
     5221 gttccagaag gaacgaatat tcttgctgca gaatgtaaag aagtttcaga aaatgagcct
     5281 ttaacacgtg aaaaattatc tccagtcatt gctgttttga aatctgaatc acgtgaagac
     5341 ggtgt
//
LOCUS       DAUCWU010000093         5313 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUCWU010000093 DAUCWU010000000
VERSION     DAUCWU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.093"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            141..1412
                     /locus_tag="VGY24_001540"
     CDS             141..1412
                     /locus_tag="VGY24_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ4284507.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            1412..2122
                     /locus_tag="VGY24_001541"
     CDS             1412..2122
                     /locus_tag="VGY24_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284508.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            2134..3354
                     /locus_tag="VGY24_001542"
     CDS             2134..3354
                     /locus_tag="VGY24_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284509.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGKGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKRACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            3608..>5313
                     /locus_tag="VGY24_001543"
     CDS             3608..>5313
                     /locus_tag="VGY24_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEQ4284510.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SR"
BASE COUNT         1789 a          799 c         1020 g         1705 t
ORIGIN      
        1 cagtaagcta ttaatttgtc atagcctata agtcattatc ttgtggcttg ttttcgacta
       61 agggctaaga atttcttgac aacaatataa aaaggtcata tacttgtctt atacaaatag
      121 tacaaaaggg gaggatagaa atgtctaaaa gaggaaaaat taaaataaca acgaaaacaa
      181 agcttattac agctagtgtt ataacgctag tattaattat aactggagta gtcttgtgga
      241 aacaacaaca aaatacgcta acagctgata tcgctaaaga accttactct actgttagtg
      301 taactgaagg gagtattgct tcttcgactt tactatcagg tactgtaaag gctttatcag
      361 aggaatatat ttattttgat gctaataaag gaaatgatgc aactgttaca gttaaagtag
      421 gtgatcaggt aacgcagggc cagcaattag ttcaatataa tacaacaaca gctcagtcag
      481 cttatgatac tgctgttagg agtcttaaca agattggccg acaaattaat catcttaaaa
      541 catacggagt tcctgctgtt agtacagaaa ctaataaaga tgaagctacc ggtgaagaga
      601 cgacaacaac agttcaacca tcagctcagc aaaatgctaa ttataaacag cagctgcaag
      661 atttaaatga tgcttatgca gacgcacaag cagaagtaaa taaagcgcag atagcgttaa
      721 atgatacagt agttatcagt agtgtctctg gaactgttgt ggaagtaaat aatgatattg
      781 atccttcttc aaagaacagt caaacacttg ttcacgtagc aaccgaagga cagcttcagg
      841 tgaaaggaac attgacagag tatgatttag caaacgttaa ggttggtcaa tctgtaaaaa
      901 ttaagtctaa agtttattct aatcaagaat ggactggaaa aatatcatat gtttcaaact
      961 atccaactga gtctaatgca ggttcaacaa cgccagcagg tagcactgga gcgggaagtt
     1021 ctacaggagc tgcctatgat tacaagattg atattataag tcctctcaac cagcttaaac
     1081 aaggtttcac tgtttctgtt gaggttgtta atgaagctaa acaggcctta gttcctttaa
     1141 cggctgttat taagaaagat aaaaaacact atgtttggac ttatgatgat gctactggca
     1201 aagccaaaaa agtagaggtg acacttggaa acgcagatgc acaacaacaa gaaattcata
     1261 aaggagtagc tgttggtgac attgttattg ccaatccaga taaaaatatc aaaccggata
     1321 aaaaactaga aggggttatt tcaataggta ccaacacaaa accggaaaaa gattctcaat
     1381 caaagaataa aaaatcaggg gtggataaat agtgtcgcat gaaaaaaaac aattaatgca
     1441 attatcaaat attgtgaaga gctatcaaaa tggggatcaa gttcttaaag ttttgaaagg
     1501 tattaattta acagtatatg agggtgaatt tttagctatt atgggtccgt ctggctcggg
     1561 aaaatcaaca ctaatgaata ttattggttt attagatcgg ccaacatccg gagattacac
     1621 attacataat actaaaatag aaatccttaa tgatcgtgaa ttagcaaaag tacgaaacga
     1681 tgaaatcggc tttgtctttc aacaattctt tctgttagct aaattaactg cattacaaaa
     1741 tgttgagttg cctcttattt atgctggtgt aaacgtctca aaaagacgtg aacaggctaa
     1801 gcaatttctt gaaaaagtag gacttggacg acgtatcaaa catctaccgt cagaactgtc
     1861 aggaggtcaa aagcaaaggg tggctatcgc acgagcttta gtaaatgacc catctattat
     1921 tttagcggat gaaccaacag gtgcactaga tactaaaaca ggtcagcaaa ttatggaact
     1981 cttgacagag ttaaacaaag aaggtaaaac aattattatg gttactcatg aaccagaaat
     2041 tgctgatttt gcaactcgga aaattattat tcgtgatggt gatatcacaa cggatactac
     2101 agctagtgtt gttattgact aggaggaaaa ataatggaaa attggaaatt cgctcttagc
     2161 tcaatttggg ggcataagat gcgctcaatc ctgacaatgt tgggaattat catcggtgta
     2221 gcagcagttg tgattattat ggggttggga aatgctatga aaaatagtgt aacaagcaca
     2281 ttttctagca agcaaaaaga tattcaactc tattttcaag aaaaaggaga agaagaagac
     2341 ctgtatgccg gattgcatac gcatgaaaat aatcacgaag tgaagccaga gtggttagaa
     2401 caaattgtta aagatatcga tggtattgat tcttattact ttaccaattc tgctacttct
     2461 actatttcgt atgaaaaaaa gaaagtagac aatgcttcga tcataggggt aagtaaagat
     2521 tattttaata taaaaaacta tgatatcgta gctggtcgta cactaacaga taatgattat
     2581 tctaattttt cgcgtatcat tcttttagat acagtattag ccgatgacct atttggcaaa
     2641 ggcaattata agtcagcttt aaataaagtg gtaagtttat ctgataaaga ttatttagtt
     2701 attggtgttt ataaaactga tcagacgcca gtttcttttg atggattatc aggcggcgct
     2761 gtcatggcaa atactcaggt agcttcagag tttggcacca aagaaatagg atctatctac
     2821 attcatgtga acgatattca aaacagtatg aacttaggaa atcaagcagc tgatatgcta
     2881 accaacatat cgcatattaa agatggacag tacgctgttc ctgataatag caaaatcgtt
     2941 gaagaaatca atagtcagtt cagtatcatg acaacagtca taggttcaat tgcagcgatc
     3001 tctcttttag tgggaggaat tggtgttatg aatattatgt tggtatctgt cacagagcga
     3061 actagagaaa ttggtttacg taaagcactt ggtgcaacac gcctgaaaat tttatcacaa
     3121 tttttgattg aatccgttgt tctaacagtt cttggaggct tgataggatt attattagct
     3181 caattaagtg taggggcttt aggaaatgcg atgacattaa aaagagcttg tatttctctt
     3241 gatgtggcct taatagcagt tttattttct gcctcaatag gtgtcttttt tggaatgttg
     3301 ccagctaata aagctagtaa gttagatcca attgaggcat tacgttatga atagaatagt
     3361 tcgtatcaat taatctatag acgctgacta gtttaacgtc tgattatatc tagtttttgt
     3421 attagcacag tagttatatc attgaagtgt tagtgcattt tttgaggtga taatatctag
     3481 caaagctatc attggtatga tgatgtggat agcgtataag tgatacatct cttatttcta
     3541 gctgaacttc ggttcggcta ggaagttgtc aggcaatgag ttgtttaaaa taaaggagat
     3601 tagttcaatg acatttttat ctgatttgat atcattaatg acaaaaatca gattatcttg
     3661 ggtaataaag gcgggtattt ttcaattatt atttgtaacg attgctaata ttgtcttatc
     3721 agaatttttc tattttatat tagacgttac tggtcaatat catttagata aagacaatgt
     3781 tgtgactttt ttaaaaaatc ctatagcact tgctttatta ggtgcctatt tatttttatt
     3841 agctgctttt attcaccttg agttttttgc tctatatcga attattgcgg atcaagaaat
     3901 tagtttctat ctttttagaa aacagttttc ttattaccta agggggcttt ggaaaacatt
     3961 ttctggttac caattattac tttttttgct ttatatccta ctgactattc cagtcttaca
     4021 tattggttta tcttctgtga ttactcaaaa gctttatctt ccagaattta ttgttgggga
     4081 attatcaaag ataactagca caaagtactt gctttatggc agtcttattc ttgtgtttta
     4141 ccttaaccta agattagtat attttttacc attgatagca atcaaccatc gtacggttgc
     4201 tcaagcatgg agagagagtt ggcaaaagac taaaaagaaa catgtattgt tatggatgaa
     4261 actttttgca atcaatggtc ttacgcttgt agtcttatcg ctagctattt ccatgattct
     4321 tatttttgtt gatatgttta atcctaaggg gaataatatt attgttcagc tgggagcttt
     4381 gacctttaca tgggagctca ttttttttac tactattttt tttaaactct gttcagcgat
     4441 gattttaaaa gaggcaattg aaccacaaaa gcaatatgat gagccaagaa gaagtaataa
     4501 ggcatatgtt gtaatcttta tcgtggtgac agtaggtttt gcttatcaat ctcttgaacg
     4561 tttaactttt tttgacacat ctcactctaa gacagttatc gcgcatagag gacttgtatc
     4621 agcaggtgta gaaaattctc tagaagccct tgaaggtgct aagaaagcag gaagtgatta
     4681 tgtagaactg gatctaatct tgactaagga taatcacttt gtggtgtctc atgataatcg
     4741 attgaagcgt ttagctggag taaataagac gattcgcaac ttaaccttaa aagaagttga
     4801 acatctaacg agtcatcaag gacatttttc agggcgtttt gtttcttttg acacttttta
     4861 tcaaaaggct aagaagttga atatgccatt acttattgaa ctcaagccaa ttggtacaga
     4921 acctggaaat tatgtcgatt tgtttttaga aacttatcat cgacttggta taagcaaaga
     4981 taataaagtc atgtctttag atttagaagt aatagaagct atcaagaaaa aaaatccatc
     5041 aattacgact ggttatatca taccaattca atttggcttt tttggagatg aatttgttga
     5101 tttctatgtc attgaagact tttcttatcg gtcttatttg tcgtcccaag ctttttggaa
     5161 taataaagaa atttacgttt ggactattaa tgatcccaag cgcatagagc attatctcct
     5221 aaagcctatt cagggaatta ttacagatca accagcttta actaatcaat tgattaaaga
     5281 cttaaaacaa gataattctt attttagtcg att
//
LOCUS       DAUCWU010000094         5223 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUCWU010000094 DAUCWU010000000
VERSION     DAUCWU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.094"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            328..530
                     /locus_tag="VGY24_001544"
                     /pseudo
     CDS             328..530
                     /locus_tag="VGY24_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            730..1110
                     /locus_tag="VGY24_001545"
     CDS             730..1110
                     /locus_tag="VGY24_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ4284511.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            1110..1958
                     /locus_tag="VGY24_001546"
     CDS             1110..1958
                     /locus_tag="VGY24_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4284512.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMANSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            2083..3291
                     /locus_tag="VGY24_001547"
     CDS             2083..3291
                     /locus_tag="VGY24_001547"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ4284513.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGRVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEATTFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDR
                     INTLDQALVIHDKHDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            3288..5165
                     /locus_tag="VGY24_001548"
     CDS             3288..5165
                     /locus_tag="VGY24_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ4284514.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDAASVLDTVLSADVKAIQLIRQ
                     YELLMANYTEDKQESLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDAANLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
BASE COUNT         1703 a          826 c         1150 g         1544 t
ORIGIN      
        1 acggtttttt gatgatatgt tgctgcatga ggaagtgagc aataggagag ctgtgataga
       61 agaaagaggg gaagaagcgt tgatgaaatt agacaaataa aaaaagacct catctagaag
      121 aggtcgtgat ataactgggg acgataggtt caattgataa aaaatcttgt tagcttacga
      181 tctatgttgt tttgaatgcc tttaactcaa catcgttaat ggattttgag ataaaaaaat
      241 aaatccaata gtctggtact ttcctatttt ttgtctgtta aaacgttttt attttataat
      301 ggataaaagg agaagaaggg cggagcgatg attaagctct atgggtttaa gtatgggtta
      361 gttggagagg ttttgcggcg gattgaacgc ctaggattta cgtttgagcg attagagcta
      421 agacaagcta gttcgaaata cttggctaag catgatgagg ccttggtgat aaaccttttt
      481 atcctggact taaaacttac ctgaccagtg gttctgtttt aattgaggtg cagtacaaga
      541 ccactaacgg gtgttttttg atataatgtc agtataagga cgactaaata taaataggtg
      601 aaaaaataga tatctgttcg agagatagca ctgtttaaaa cgttatgcaa aaatagtagt
      661 taggggctac tttaataatt ttcaaatcta tgctataatg gatcatattg ataccaagga
      721 gaataaaaaa tgcaattagt tcgtgaaaaa gaatttgtca accagtacca ttatgatgct
      781 agaaatttag agtgggaaaa agaaaacgga acaccagaga ccaattttga agtgactttt
      841 cagttgattg ataaagatga gcagcaaaaa gaaacagtaa ttgtatctgt attgcaattt
      901 gttattgtca aagaagaatt tgttattagt ggggtcatct cacaaatggt tcgtatttta
      961 gatcgtttag tggataaacc aagcgaattt acgcaagagg aagttgaatc attagctgct
     1021 cctttgctag acatggttaa gcgattgact tatgaagtca cagaaattgc cttggatcgt
     1081 ccaggtattc atttggagtt taaaaattaa tgaaattagc agtaattact gacagtacag
     1141 caacactacc aacagatctg aaacaagata aagctatttt tagccttgat ataccagtta
     1201 tcattgacga tgagacctat tttgaaggtc gaaatttgag tatagatgat ttttatcaaa
     1261 agatggctaa ctcccaaaat cttcccaaga caagtcagcc aagtttatct gaactagata
     1321 atcttttagg acttttgtct tctaagggtt atacacatgt tattggcttg ttcttagctg
     1381 gaggaatctc aggcttttgg caaaatattc aatttttagc tgaagaacat cctgagattg
     1441 aaatggcatt tccagacagt aagatcacat ctgctccttt aggtagtatg gtaaaaaatg
     1501 tgctagattg gtcacgtcaa ggaatgacat ttcaagctat tttaaacaag ctgcaagaac
     1561 agattgatgg gacgacagcc tttattatgg ttgatgacct taatcatctg gttaaaggcg
     1621 gacgcctgtc aaatgggtct gctcttcttg gaaatctttt gagtattaaa cctattttac
     1681 gctttgatga agagggaaaa atcgttgttt atgaaaaagt gagaacagaa aagaaagcca
     1741 tgaaacgctt ggttgagatt ttaaatgact tgatagcaga cggtcagtat aatgtgtcta
     1801 ttattcactc aaaagcacaa gacaaagctg attatttgaa acgactctta caagacagtg
     1861 gttatcaata tgacattgaa gaggttcatt ttggagcggt gattgctacc catttaggag
     1921 aaggagccat tgcatttggc gtaacaccga ggctttaatt gtcgattaga aaaaatagtt
     1981 aattgcaaga atcactaaaa caataacaaa atgtgataga acccaagtag cctaatcaca
     2041 ctttagattg ggctagttgg atgataggtt agaaaacaac gtatgaaatt aatgacaatg
     2101 ccttctgagt ttcagaaggc tttacccata ttgactaaaa taaaagaagc tggttatgaa
     2161 gcctattttg tgggaggaag cgtcagagat gttttactcg agcgtcctat tcatgatgta
     2221 gatattgcaa ctagttccta tcctgaagag actaaagcga tttttaacag aactgtcgat
     2281 gttggcattg aacatggtac agtgctcgtg cttgaaaatg gcggtgagta tgaaatcaca
     2341 acctttagga cagaagatgt ctatgttgat tatcgccgtc ctagtcaggt ttcttttgtg
     2401 cgctctttag aggaagactt aaaacgaaga gactttaccg tcaatgcctt ggccttggat
     2461 gaaaatggtc gagttattga taaatttaga ggactcattg atttaaagca aaaacggtta
     2521 cgagcagtgg gtaaagcaga ggaacgtttt gaagaagatg cactacgcat tatgagaggc
     2581 tttcgatttg cggcgagtct ggattttgac attgaagcta caacttttga agctatgagg
     2641 agccattcac ctctattgga aaagatttct gtggaacgtt cttttactga atttgataaa
     2701 ttattgatgg caccgcactg gcgaaaaggg atttctgcca tgattgcttg tcaggcttac
     2761 gactatttac caggcttgaa gcagcaggaa gcaggcttga atcatctgat agtttctttg
     2821 aaagataact ttacctttag tgattatcac caagcttggg cttatgtgat gattagttta
     2881 gccattgagg atcctaaaag cttcctaaaa gcatggaaga cttcaaatga ttttcagcgt
     2941 tatgtcacaa agttgattgc tctttataga attcgtcagg aaagatcttt tgaaaaattg
     3001 gatatttacc agtacggcaa gaaaatggcg agtctagtgg aagacctccg aaaagctcaa
     3061 agcttatccg ttgacatgga tcgtattaat actttagatc aggcactggt tattcatgac
     3121 aaacacgata tcgtccttaa tggttctcac ctgattaagg attttgggat gaaaccaggt
     3181 cctcagctag ggcttatgct tgagaaagtt gaattagcga ttgtagaggg gcgcctagat
     3241 aatgatttca ctacaattga agcatttgtt agagaggaat tagcaacatg agccattttt
     3301 tggtggagaa attaacaaaa acggtgggag ataagaccgt ctttcaagat atttccttta
     3361 ttattcatga ctttgaccgt attgggatta ttggggttaa tggaacagga aaaacaactc
     3421 ttttagatgt gttgtcggga aggcttggtt ttgacggaga tcattcacca ttttctaaag
     3481 ccaatgatta caaaatcgct tacttaacac aagaccctga atttaacgat gcagcttctg
     3541 ttttagatac ggtattatca gctgatgtta aagctattca actgattcgt cagtacgaac
     3601 tattgatggc gaattatact gaggataaac aagaaagctt agagtccctc atgtctgaaa
     3661 tggatcgctt agatgcttgg tctattgaaa gtgatgtcaa aacagtccta tcaaaattag
     3721 gcatcacgga tttggaacaa aaagtgggag acctatcagg tggtatgaga agacgtgtcc
     3781 agttagctca agtgttgcta ggagctgctg acttactctt gttggatgag ccgaccaacc
     3841 atctcgatat tgacaccatc gcttggttaa caacctatct caagacagct aaaaaaaccg
     3901 tcctttttat tacccatgac cgttatttcc ttgatcatgt ggctacccgt atttttgaat
     3961 tggataaagc gggactgaca gaatatcaag gcaattacca agactatgtg cgcttaaaag
     4021 cagaacaaga cgaacgagat gctgctaatt tacacaagaa gaagcaactc tataaacaag
     4081 aattagcttg gatgaggacg cagccacagg cgcgtgcaac caaacagcag gctagaatca
     4141 atcgtttcac tgacttgaaa aaagaagttc accaagacag ttctgcggat aaattggaga
     4201 tggctttcga aacgagccga attgggaaga aggtgattca ctttgaagac ctttcttttg
     4261 cttatggcga ccgacagttg attaaggatt ttaacttgat tattcaaaat aaagatcgta
     4321 ttggaattgt tggagataac ggcgtcggaa aatctaccct gttaaatatc atcaacggtg
     4381 atctcaaacc aacttctggg aagcttgata ttggagatac tattcgtgta ggctatttct
     4441 ctcagcagct caaagatatg gatgagacca aacgggtcat taattacctt caagaagtgg
     4501 ctgatgaggt taaaacaagc gtgggaacaa cgagtatcag tgagctttta gaacaatttc
     4561 tttttccgag atcaagtcat ggcacgctga ttgctaaatt atctggtggg gagaaaaaac
     4621 ggctctactt gctaaaactg ctcattgaaa agccaaatgt tcttctcctt gatgagccaa
     4681 caaatgattt agacattgcc accttgacgg tcttagaaaa ctttttagct aactttgcag
     4741 gaccagtcat tacggttagt cacgaccgtt atttcctaga caaagtagcc actaagattt
     4801 tagcttttga agaaggcgat attcgtattt tttatgggaa ttacagtgat taccttgatg
     4861 aaaaagcttt tgaaaaagaa acagttgagg ctgacttagc taaagcaata gtcactgaag
     4921 aagtgccact tcctccgaaa gaggagaaaa agcgcatgtc ctaccttgaa aaacaagaat
     4981 gggctcagat agaagataaa atagcaacta ttgaggctaa tattgaagaa attgaaaacc
     5041 aaatgttaac tgtagttagt gattatggcc agttagctca attacaaaag gaactcgatc
     5101 agcgcaataa tgatttatta ttggcgtatg aacggtttga gtatttgagt ggactagaca
     5161 tataagtata ctatggtaat gttatgttta taataaacaa gtgataatag aaaagaaggg
     5221 ctc
//
LOCUS       DAUCWU010000095         5221 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUCWU010000095 DAUCWU010000000
VERSION     DAUCWU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.095"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(236..544)
                     /gene="rpsJ"
                     /locus_tag="VGY24_001549"
     CDS             complement(236..544)
                     /gene="rpsJ"
                     /locus_tag="VGY24_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ4284515.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(743..2032)
                     /locus_tag="VGY24_001550"
     CDS             complement(743..2032)
                     /locus_tag="VGY24_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ4284516.1"
                     /translation="MIYSRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(2420..3436)
                     /gene="adhP"
                     /locus_tag="VGY24_001551"
     CDS             complement(2420..3436)
                     /gene="adhP"
                     /locus_tag="VGY24_001551"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ4284517.1"
                     /translation="MKAVVVNEASTGVEVVDHEMPSIGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLQVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(3720..>5221)
                     /locus_tag="VGY24_001552"
     CDS             complement(3720..>5221)
                     /locus_tag="VGY24_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEQ4284518.1"
                     /translation="LGHSAAIHTADAELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNA
                     FLPSLTLGCGSYGRNAVGDNVSAINLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYL
                     QKCRDVERVMIVTDHAMVELGFLDRIIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGT
                     ELMRTFKPDTIIALGGGSPMDAAKVMWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELG
                     KKTKFVAIPTTSGTGSEVTPFAVISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGF
                     IAADTGMDVLTHATEAYVSQMANDFTDGLALQAIKIVFDNLEKSVKTADFEAREKMHN
                     ASTMAGMAFANAFLGISHSMAHKIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWP
                     KYNYYRADEKYQDIAKLLGLPASTPEEAVESYAKAVYDLGCRVGIKMNFKDQGIDENE
                     WKEHSRALAYLAYEDQCSPANPRLPMVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         1609 a         1151 c          924 g         1537 t
ORIGIN      
        1 ccttttctta ataatatttc aaaaagtttt ttatgagcgt tttttgtgct caaaaaagtt
       61 ttttacgagc gttttttgtg ctcatagcaa gatacaaaga gcgtcaaact caaaataaaa
      121 atagaaaatc taacacaaaa acttctgttt cgaggaagat ttatcttttt catgtagagt
      181 ttagctcgtg ttcaagttcc atcaaacact aactgtactt gcaaaatctc accgattaaa
      241 gtttgatttc tacgttgaca ccacttggaa gatcaagttt catcaaagca tcaacagttt
      301 tttgtgttgg attgatgatg tctaccaaac gtttgtgtgt acgcatttca aattgttcgc
      361 gagaatcttt gtatttgtga gtcgcacgaa taattgtgta aagactgcgt tcagttggaa
      421 gtggaactgg tccagcaact gtagcacctg tacgtgttgc agtttcaacg attttttctg
      481 ccgctgtatc aagtgtacgg tgttcgtacg ctttcaaacg gatacggatt tttttgtttg
      541 ccatcttttc tcctctcgtc tatttacgat aataggctag ctcctcaaga aaaccgacac
      601 ctgttgcgtg gcaatgcaac cgagcgtgtc gcaacctctt gtatcattgc taaagctgta
      661 tttttacagc accacaatat aataacagaa tcatttagtc attgcaagct ttttaggcat
      721 ttttttcaaa aaaactacct tcctaatgaa aggtaatttc ttggtacttt ttaaattgtt
      781 tagagagaat aaaccaccga ctggtattat ctaacactgt cgccatccag acccctatga
      841 gtccatattg ccaaaccaca ccaatcacat aaccaaggcc aatccgaatc acccacattc
      901 ctatggttgt tgcatagaaa ggaagctttg cttttcccaa accctgccag acggctgtat
      961 aaactaaagt gcctgccgtt gcgggagctc ctagtaaaga aaaaagtaaa acaaccatgg
     1021 cggacctttg agcatcagta ttctgagtaa atagcggcaa gaggctagga cctagcagat
     1081 aggtcaaggc tcccataacc agcatcatga gggtggataa aataaaagct tctctaataa
     1141 tataccttat ctcagtcacc ttaccgcccc ccagttgcct agccactaga ataattgttg
     1201 ccgttgccat ggctaagcca ggcatgtaat taaactgagt caaggtttct ccaatagcat
     1261 tacctgccaa ggccgttgtc ccaaagcgaa caacaatgcc aataattagg acatctcctg
     1321 ctctcatcat caaacgttca ccagcagcag gtagtgataa atcaaaaata attttgtcca
     1381 aaggtctcat caatcgtttc gcaacctgtt taataggaat aaactgacaa agtaaaaaca
     1441 cacccactaa acgagataaa accgtcgccc aagcaacacc aagaagacca aatccccata
     1501 catagataga caaggcagaa aaaatggcat tgagtacgtt tattaataag ctcacctgca
     1561 taggaatctt aggataccct tgagctcgaa caatagctcc taagctagtt aaaagtccta
     1621 gactaacaat cataccgccg acaatggata gatattgccc tccaaccaat gttacagaag
     1681 cttcagctcc tagccattct aacacctgat gatgtccaac aactgataac aaccctaagc
     1741 cgaccgaaag gaggagtgtt acttgcaaga cacctgccat atagtttaat tgtttgttct
     1801 gattattttc tccaatactt cgagcaatca gactagatac agcagctccc agcgcgataa
     1861 agagggactg ataaatactg ataatgttgt tagctatgga gacgccagag actgctacaa
     1921 gtccaatttg agccaccagg taattatcaa ccattcccat taacatctgt aaaatatttt
     1981 ctatcataga aggcagagct aaagagaaaa tttttcttct actatatatc atttatgtcc
     2041 cctaataaaa aattcttgat caaacgattt tttcaaacac agtcactgga ctcattcagt
     2101 tattttggtc cattaatgaa ttcataaagt ctatcttaat agcagagtcc caagatacac
     2161 gacacttagt gctacagcaa caattgacca catgccaatc cttaacattt acctaacgtt
     2221 tataactaag aagacgaaga acaaaaggac tccaaacgaa ttttttctat tatataatat
     2281 attcttcatt tagcaacttt caatgtatca aaatagcact ctcgagtcaa aaatgttttc
     2341 tgatactcga gagtgctatt ttatttggaa tcaagtttaa acaacttagg cttatctcat
     2401 atttaataca tttgtatgtt tagctgatga aatctaagac cttgcgccct tggattaaac
     2461 cacgttccat ttcatcaaag acttctggag cagtgtcaac tggaactttt tcgacaaccg
     2521 gaacaaccag accttctgca ccaaaggcaa aggcttcttc caagtcttta cgtgtcccta
     2581 caagtgatcc gaccactttg ataccatcta aaacagtttt tacgatagac agttccatgt
     2641 attctgatgg aagacctacg gctacaactg ttccaccggc acggacacta tcaattgctt
     2701 ggttaaaagc aactttagaa acagccgtta ccacaacacc atgtgctcca ccggtttttt
     2761 cttgaatata gcctggaaca tcttcaattt ctttgccgtt aactaaaata tctgcgccaa
     2821 cttctttagc taactcaagt ttatcattat taatgtccac tgcgacaaca tgtgcattaa
     2881 agactttttt agcgtattga acagccagat ttccaaggcc acctgcccca aagataacaa
     2941 tccattggcc tggagcagca cctgcctctt taatagcttt ataagtagtg acacccgcac
     3001 atgtgattga tgaagcttgg gcaggatcaa gtccttcagg gacttttaca gcgtaatcag
     3061 ctgttacgac agcatattcg ctcattcctc catcaacgct gtaacctgca tttttaacag
     3121 aacggcagag agtttcacga ccagtcgtac aatattcaca atggccacaa ccctcaaaga
     3181 accaagcaat agagacacga tcaccaactt gtaatgaggt tacaccttca ccaatttctt
     3241 cgacaatacc aatgccttca tggcctagaa tacgcccagg aacttgtcca aaatcgccat
     3301 gcgcaacgtg caaatctgta tggcaaacac cacaatattc gactttaaca agtgcttctc
     3361 cgtgcccaat tgatggcatt tcatgatcaa caacttcaac acctgtacta gcttcattaa
     3421 caactacggc tttcatataa gtcctccata ttttaatgtt taattgttac ttctgtaacc
     3481 gtttacaatt aagattgtat atcttttcac aattattgtc aactcatttt cgaaagtttt
     3541 ctgaaaatta agttaactag ttaagttgtt gaaacctctc tttgattatt ataagcaaaa
     3601 tcctttcttc cttttagagg ggattttggg tatgttgtat tcaactcaat acacgaaaaa
     3661 aacgacctct tatggttcta gtcataagag gtcgttgcat cacaacactt taaaagagat
     3721 tatttacgac gtcctgggcg ctctgcgtaa ccatagtaag catcctcaat gatttcttgc
     3781 atatggtcaa ccattggtaa gcgtgggtta gcaggcgaac attggtcttc ataagcaagg
     3841 taagccaagg cacgtgaatg ttctttccac tcgttctcat caattccttg atctttgaag
     3901 ttcatcttaa tgccaacacg gcaacccaga tcataaacag ctttcgcata agattccact
     3961 gcttcttctg gtgtagaagc tggaagccct aacaatttcg caatgtcttg gtatttttca
     4021 tccgcacggt agtaattata cttaggccat gtcgccgttt tagctggacg agttccatta
     4081 tagcggataa catacggcaa aagaattgca tttgtacgtc cgtgtacggt atggaattgt
     4141 gcgccaattt tatgtgccat tgagtgagaa atacctaaga atgcattggc gaaagccata
     4201 cctgccatag ttgacgcatt gtgcatcttt tcacgtgctt caaaatcggc tgttttaact
     4261 gatttttcaa gattatcaaa aacaatttta atggcttgaa gagctaaacc atccgtaaag
     4321 tcattggcca tttgtgacac ataagcttcg gttgcgtgtg tcaaaacgtc cataccagta
     4381 tcagcagcaa taaagcctgg aacagtcaat accaaagctg ggtcaacaat ggctacagtt
     4441 ggtgttaatg agtaatcagc gattggatat ttacgattat ttgctttgtc agagataacg
     4501 gcaaatggtg tcacctctga acctgttcca gaagttgttg ggatagcaac aaattttgtt
     4561 ttcttaccaa gttctgggaa tttgaacgca cgtttgcgga tatccatgaa tttttgaacg
     4621 aggtcatgga aatcaacttc tggttgttca taaaagagcc acataacttt ggctgcatcc
     4681 ataggagatc ccccgccaag ggcaataatg gtgtctggtt tgaaggtacg cattaattca
     4741 gtacctttca taacagttgt aatgtctgga tctggttcta cttcagcaaa gatctgataa
     4801 acaactttat tgcgacgaag atcaagttgt tcaatgatac gatccaagaa accaagctca
     4861 accatagcgt gatctgtaac aatcatgaca cgttcaacgt cacgacattt ttgcaaatat
     4921 tgaattgaat cacgttcgaa gtatgttttt gaaggaactt taaaccattg catattattt
     4981 ctacgtcttc ctactttttt gatgttcaag agatttatag cactaacgtt atcaccaact
     5041 gcgttacgtc catacgaacc acaaccaaga gtcaatgatg gcaagaaagc attgtaaaca
     5101 tccccgatac caccaaatgt agaaggtgaa ttccagatga cgcggatagc acggattcta
     5161 gttccaaatt cttttgccaa ctctgcatct gctgtatgaa tggcagctga gtgaccgagt
     5221 c
//
LOCUS       DAUCWU010000096         5154 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUCWU010000096 DAUCWU010000000
VERSION     DAUCWU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.096"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            101..1649
                     /locus_tag="VGY24_001553"
     rRNA            101..1649
                     /locus_tag="VGY24_001553"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VGY24_001554"
     tRNA            1701..1773
                     /locus_tag="VGY24_001554"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            2072..4974
                     /locus_tag="VGY24_001555"
     rRNA            2072..4974
                     /locus_tag="VGY24_001555"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5060..>5154
                     /gene="rrf"
                     /locus_tag="VGY24_001556"
     rRNA            5060..>5154
                     /gene="rrf"
                     /locus_tag="VGY24_001556"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1464 a         1062 c         1478 g         1150 t
ORIGIN      
        1 agacgaacca aacgtgaggg tgatatggag acatattacc cgtcaagaaa cgagaaataa
       61 atctgtcagc gacagaagga acgagtaagt tcaaacgcaa attaaagaga gtttgatcct
      121 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gagaactggt
      181 gcttgcaccg gttcaaggag ttgcgaacgg gtgagtaacg cgtaggtaac ctacctcata
      241 gcgggggata actattggaa acgatagcta ataccgcata agagagacta acgcatgtta
      301 gtaatttaaa aggggcaatt gctccactat gagatggacc tgcgttgtat tagctagttg
      361 gtgaggtaaa ggctcaccaa ggcgacgata catagccgac ctgagagggt gatcggccac
      421 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttcggcaa
      481 tgggggcaac cctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaagc
      541 tctgttgtta gagaagaatg atggtgggag tggaaaatcc accaagtgac ggtaactaac
      601 cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc ccgagcgttg
      661 tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgaa gttaaaggca
      721 ttggctcaac caatgtacgc tttggaaact ggagaacttg agtgcagaag gggagagtgg
      781 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg gcgaaagcgg
      841 ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata
      901 ccctggtagt ccacgccgta aacgatgagt gctaggtgtt aggccctttc cggggcttag
      961 tgccggagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca
     1021 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg
     1081 aagaacctta ccaggtcttg acatcccgat gcccgctcta gagatagagt tttacttcgg
     1141 tacatcggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
     1201 gtcccgcaac gagcgcaacc cctattgtta gttgccatca ttaagttggg cactctagcg
     1261 agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat
     1321 gacctgggct acacacgtgc tacaatggtt ggtacaacga gtcgcaagcc ggtgacggca
     1381 agctaatctc ttaaagccaa tctcagttcg gattgtaggc tgcaactcgc ctacatgaag
     1441 tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta
     1501 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taacctatta
     1561 ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta
     1621 tcggaaggtg cggctggatc acctcctttc taaggaaatg gaacacgttt atcgtcttat
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccatcaggat acaatcctac taaacttaat
     1801 acaagtgaag ttgaacacgc aactcacttc ctaggaaaat agacaatctt cccttgtgtg
     1861 caaggcccac atggtcagat tcctaatttt ctacagaagt ttcgctaaag cgagcgttgc
     1921 ttagtatcct atataatagt ccattgaaaa ttgaatatct atatcaaatt ccacgatcta
     1981 gaaatagatt gtagaaaagt aacaagaaaa taaaccgaaa acgctgtgac tatttaataa
     2041 gttttctagt ttaaaaaaac taggttaata aggttaagtt aataagggcg cacggtggat
     2101 gccttggcac tagaagccga agaaggacgt gacaaacgac gaaatgcttt ggggagctgt
     2161 aagtaagcgc tgatccagag atgtccgaat ggggaaaccc actaactaat ggttagtatc
     2221 cataactgtt aaggttatga gaaggaagac gcagtgaact gaaacatcta agtagctgca
     2281 ggaagagaaa gcaaacgcga ttgccttagt agcggcgagc gaaacggcag gagggcaaac
     2341 cgaagtgttt acacttcggg gttgtaggac tgcgacgtgg gactttaaaa ggatagaaga
     2401 attacctggg aaggtaagcc aaagagagta aaagcctcgt atttaaaatt cttttgagcc
     2461 ctagcagtat cctgagtacg gcgagacacg tgaaatctcg ttggaatctg ggaggaccat
     2521 ctcccaaccc taaatactct ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg
     2581 aaaagcaccc cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg
     2641 agcccgttaa tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgatatgat
     2701 gcgaggttaa gttgaagaga cggagccgta gggaaaccga gtcttaatag ggcgatttag
     2761 tatcatgttg tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgaggtaa
     2821 aactcactgg aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc
     2881 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
     2941 cgtcgatgtt aagtctcttg gaggtagagc actgtttggg tgaggggtcc atctcggatt
     3001 accaatctca gataaactcc gaatgccaac gagatatcat cggcagtcag actgcgagtg
     3061 ctaagatccg tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataact
     3121 gttaagtgga aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct
     3181 attcattcaa agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc
     3241 ggggctaaaa cagtttaccg aagctgtgga tgacacaaaa gtgtcatggt aggagagcgt
     3301 tctatgtgtg aagaaggtgt accgtgagga gcgctggaac gcatagaagt gagaatgccg
     3361 gtatgagtag cgaaagacag gtgagaatcc tgtccaccgt aagactaagg tttccagggg
     3421 aaggctcgtc cgccctgggt tagtcgggac ctaaggagag accgaaaggt gtatccgatg
     3481 gccaacaggt tgatattcct gtactagagt atatagtgat ggagggacgc agtaggctaa
     3541 ctaaaccgga cgattggaag agtccggcta agcagtgagg tgtaagatga gtcaaatgct
     3601 tatctttata acattgagct gtgatgggga gcgaatttta gtagcgaagt tagtgatgtc
     3661 acactgccaa gaaaagcttc tagcgtttaa tgatactcta cccgtaccgc aaaccgacac
     3721 aggtagtcga ggcgagtagc ctcaggtgat cgagagaact ctcgttaagg aactcggcaa
     3781 aatgaccccg taacttcggg agaaggggtg ctgacttagg tcagccgcag tgaataggcc
     3841 caagcaactg tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg
     3901 tgacgcctgc ccggtgctgg aaggttaaga ggagggttta gcgcaagcga agatctgaat
     3961 tgaagcccca gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     4021 cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac
     4081 tcggtgaaat tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc
     4141 atggagcttt actgcagttt gatattgagt atctgtacca catgtacagg ataggtagga
     4201 gccattgact tcgggacgcc agtttcgaat gaggcgttgt tgggatacta cccttgtgtt
     4261 atggctactc taacccagat aggttatccc tatcggagac agtgtctgac gggcagtttg
     4321 actggggcgg tcgcctccta aagagtaacg gaggcgccca aaggttccct cagattggtt
     4381 ggaaatcaat cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga
     4441 gcagggacga aagtcgggct tagtgatccg gtggtaccga atggaagggc catcgctcaa
     4501 cggataaaag ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg
     4561 gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt
     4621 tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc
     4681 ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac
     4741 cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat
     4801 gtagggaagg gataagcgct gaaagcatct aagtgcgaag cccccctcaa gatgagattt
     4861 cccatgattt tatatcagta agagccctga gagatgatca ggtagatagg ttaggagtgt
     4921 aagtgtagcg atacatgtag cggactaata ctaatagctc gaggacttat ccaaaaagaa
     4981 atattgacaa cgttacggat tcttgttaga ctatagatat tcaattttga gtgggttatg
     5041 aaaatagcac atttacaagt taagtgacga tagcctagga gatacacctg tacccatgcc
     5101 gaacacagca gttaagccct agaacgcctg atgtagttgg gggttgcccc ctgt
//
LOCUS       DAUCWU010000097         5093 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUCWU010000097 DAUCWU010000000
VERSION     DAUCWU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.097"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(323..1519)
                     /locus_tag="VGY24_001557"
     CDS             complement(323..1519)
                     /locus_tag="VGY24_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ4284519.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPACKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCNVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGAASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            1685..2344
                     /locus_tag="VGY24_001558"
     CDS             1685..2344
                     /locus_tag="VGY24_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ4284520.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            2366..4648
                     /locus_tag="VGY24_001559"
     CDS             2366..4648
                     /locus_tag="VGY24_001559"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ4284521.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSIIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(4728..4949)
                     /locus_tag="VGY24_001560"
     CDS             complement(4728..4949)
                     /locus_tag="VGY24_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284522.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
BASE COUNT         1683 a         1033 c          861 g         1516 t
ORIGIN      
        1 gtattataac attttttatt agctctcact atcaacagat caatcttaac atgacaaagc
       61 ctatattctg atgttaatcc aattgtcatg tatctaaacg tgcttaatcc aattgtacct
      121 actaaaaata aggccacatt ttctctttca cgaatggctt ttgcgagtca tttttattga
      181 catgtacgaa tagcaaacta gaattatttc aaagcgctat agtaatggct ttacccacag
      241 ctaatcccgg ctcataggaa ctatctacag ctgttaagat acgtttgtat ttaagtataa
      301 gactaaactc cttttcttgt ccttaagaca caacctgtct tgtctttgtt tttaataagt
      361 aaagcaaggt acaaatcagt ggaatcacag cagctaacca gaataagtgc ctaaaggaag
      421 ccgcaccact accaagtgcc aaatctttaa tcaagcccaa gaggtaaggc ccagcaccta
      481 aacctaaatc aagaccaatc atataagtag acatagccgt attaaagcgg tgctcatcca
      541 ctccttgtat ggaggcggct tgaccacaag acataaaagt gccataacca aagccaatca
      601 aggctcctga aagcaaatag cttcctccag acgatacact acctagcaag aagagtccca
      661 ttgctaaaaa aagataactt gggtacaaga cccatttatc gcctttagca tccattaaac
      721 gtcccattgc tggacgagtg atggtaataa ttaaggcata gaccacaaag aaataagcgc
      781 ctactgttgt caaatgaatt tcagacgtgt ataatttttg aaatcctaaa actgaagcat
      841 aggcaatccc cattaaaaag gcgattgcag tgataaacaa ggcttttttc tcaatgaaac
      901 tatccacagt ccacgatttg gttttagcca attgctccgc atttaaagac atgtttttga
      961 ctggaaaagc aaaagcacca acaaccacac acccaatcaa gacgttacat aagacaataa
     1021 tcatgcgaaa atcaatatga agattatcca acataaaggt tcctacaaaa gggccaatag
     1081 ctgctgctag actagttgac aagccataaa aattaattcc ttctcctctt ttacaagctg
     1141 gaatataggc tgttacaatg gtatttgtcg cagttgatac cacaccataa ccaaatccgt
     1201 ttaagaaacg aaccaagtac atcatgctaa tcgttggcat ataaaagtag gctaaggtcg
     1261 ttaagaggta aaaaatagca cctcctctta aaaccaaacg acgaccaaac acctccaact
     1321 gctttccaaa aatcaaacgg gctaataaag tccctagaat ataaatccct gttgccaatc
     1381 ctgcctggct agtctgagct cctagctctc tagtcgccac aaaggcaata ataaccgtaa
     1441 aaagatagta gaccatatag actataaagt ttattactgt gatggccaca aaatgtttgt
     1501 taaacaattt ttcttccatt gaaaatgaac tcctctctat tttcataaac attttacaaa
     1561 aaaagcagaa tataatatat ttcaatggta taatataagt gttactatca aagggcatta
     1621 gcaaatgtta atttctcttg aagatacaaa ttatacattg gtaaaagaat atttggagaa
     1681 aagcgtgaat catcacattt tgcaacggta tattgataat cataactttc ctatcattga
     1741 aaaaagttat cacaagtact tgacttttga aagtctcgaa gaggacttca cttatatttt
     1801 aaaagacggc attgtgaaac agagtgttct ttcaaaatat ggaatggaat ttaatttgag
     1861 atatgttaca ggacttgaaa ttacttctgt tctcaacact ggttattcaa aagatatggg
     1921 agagccttat aatgttcgca tcgagtcaga gaaagctagt ttttacaagg ttcgccgctc
     1981 agcattttta aaagatataa acgaagatat tgaactacaa ggatatgtga aggactttta
     2041 ccataatcgt cttcaaaaat ccatgaaaaa aatgcaatgt atgttaacta acggccgtat
     2101 cggtgccatt tctactcaaa tatatgactt aatgacctta tttggcgaag aactaccaaa
     2161 tggccagatt ttaattaatt ttgtcattac taatgaggaa ctcggtaaat tctgtggcat
     2221 ttctaccgct agcagcgtca gccgtattct aaaacagttg aaagaaaaaa acatcattcg
     2281 cattgataaa caacatatta tcattaccaa tcttgacaaa ttaaaagata atatcgtttt
     2341 ctaagccatt agaaaggaaa ctcctatgcg ctataatcaa ttttcctata ttccaactag
     2401 tctagaaaga gcggctgaag aattaaaaga attgggcttt gatctggatc tgcaaaaaac
     2461 tgccaaagct aacctagaaa gctttttacg taaactcttc ttccactacc ctgacagtga
     2521 ttatccttta agtcatctaa tcgccaaaaa tgacatggac gcattgagct tttttcaatc
     2581 agagcaagag ttatccaaag aggtttttga tttactggcc ctgcaagtcc ttggctttat
     2641 tcctggagtt gattttacag aggccgatgc tttccttgat aaactggcct ttcctatcca
     2701 ctttgacgaa acagaaatta tcaagcatat ccaccactta ttagcgaccc gttgtaagtc
     2761 tggcatgacc ctgattgatg atttggtcag ccaaggaatg cttactatgg acaatgatta
     2821 ccacttcttc aacggtaaat cattagctac ctttgacacg tcacagctga ttcgagaagt
     2881 tgtttacgtt gaagcacctc ttgataccga tcaagatggc caacttgatc ttattaaggt
     2941 taatatcatt cgtcctcaat ctcaaaaacc attaccaact ttgatgacac ctagtccata
     3001 ccatcaaggt atcaatgaag tagccaacga caaaaaactt tatcgtatgg aaaaagagtt
     3061 agtcgttaaa aaaaggagac aaatcactgt cgaagacagg gattttattc ctcttgagac
     3121 acagccttgc aaacttccta taggtcaaaa tctagaaagc tttagctaca ttaactccta
     3181 tagcctcaac gattatttcc tcgcacgtgg gtttgctaat atctatgttt caggtgtggg
     3241 aactgctggt tccactggct ttatgacgag tggagactat gctcaaattg aaagctttaa
     3301 agccgttatt gattggctaa atggaagagc cacagcctac accagccact caaaaaccca
     3361 ccaagtgaga gctgattggg caaatggtct agtttgtacg actggaaagt cctacttggg
     3421 aactatgtct actggtttag ccactacagg cgtagacgga ttagccatga ttattgcaga
     3481 atcagctatt tcttcttggt acaactacta ccgcgaaaat ggcttagttt gtagtcctgg
     3541 tggctatcct ggagaggatc tagatgtcct aactgagctc acttactcgc gtaatttatt
     3601 ggctggcgat tatcttcgcc ataatgatcg ttatcaagaa ttgctaaacc aacagtctca
     3661 ggcgttagac cgacaatctg gtgattataa tcaattttgg cacgaccgca attacttaaa
     3721 aaatgctcat caaataaagt gtgatgtcgt ttatactcat ggcttacaag actggaatgt
     3781 caaaccaaga caggtctatg agattttcaa tgcgcttcct tccacaatca acaagcacct
     3841 ctttttacat caaggtgagc atgtttacat gcataattgg caatccattg attttcgcga
     3901 aagtatgaat gcccttttat gtcaaaaact cttaggccta gctaatgatt ttagccttcc
     3961 tgaaatgatc tggcaggata acacctgccc acaaaactgg caagagcgta aagtctttgg
     4021 aacctcaatc atcaaagagc ttgacctcgg tcaggaactg cttttaattg ataatcacta
     4081 tggtgaagat gaatttaaag cttacggtaa agatttccgt gcgttcaaag cagctctctt
     4141 taaaggcaag gccaatcaag ccctgattga tattttactg gaagaggact tacctattaa
     4201 cggtgaaatt gtcctacagt taaaagtgaa atcaagcgaa aataaaggac ttctatctgc
     4261 acaaatcctt gactatggta agaaaaaacg cttgggcgat cttcctattg ctctcaccca
     4321 atcaagtatt gacaatgggc aaaatttctc tagagaaccc cttaaagagc taccatttag
     4381 agaagattct taccgtgtta tttcgaaagg tttcatgaat ttacaaaatc gcaataattt
     4441 gtcgtcaatt gaaactattc ctaataataa atggatgact gttagacttc ctttgcaacc
     4501 aactatctat catctagaaa aaggagatac ccttcgagtc atcttataca caaccgattt
     4561 tgaacatact gtccgtgaca acagcaacta tgctttaact atcgatctca gtcagtctca
     4621 attgattgtg ccaatagcat caaattaaca aagaaaagtg cttagcaata taactaagca
     4681 cttttttgtt aattggtctt actaatagca aacagaaaac gatcgtctca gttcataaat
     4741 tccgaatcta ttcctaaaaa aatatcatca acggaaaccg ctaatgcttg tgcaatacct
     4801 tttagcttct catattttgc tcgccttagc tttttagtat ctttttcata cctagcaatc
     4861 gttctgacag aaatgcctgt taagtgagac aattcctgct gtgtcatttt ctttgttgtt
     4921 cttaactctt ttaaagttga tttggtcaca attttccctc cacactaaaa tgagattagt
     4981 taatcatgtt aagtttatta taaacttcgg tttttatgaa gtcaagtttt tagagagttt
     5041 ttagaccatc ttttacgata ccttttgctt taacctcttt tatggtatca tat
//
LOCUS       DAUCWU010000098         4998 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUCWU010000098 DAUCWU010000000
VERSION     DAUCWU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.098"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..596
                     /locus_tag="VGY24_001561"
     CDS             <1..596
                     /locus_tag="VGY24_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ4284523.1"
                     /translation="LELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQPWKF
                     VVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDDLIG
                     YYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTTNEI
                     LDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            721..2130
                     /gene="pepV"
                     /locus_tag="VGY24_001562"
     CDS             721..2130
                     /gene="pepV"
                     /locus_tag="VGY24_001562"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ4284524.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKNDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNLDFGFSPDAEFPIINGEKGNITEYLHFAGDNNGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLDYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(2198..3574)
                     /gene="mnmE"
                     /locus_tag="VGY24_001563"
     CDS             complement(2198..3574)
                     /gene="mnmE"
                     /locus_tag="VGY24_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ4284525.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPNTGTIVDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    3746..3881
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3910..4410
                     /locus_tag="VGY24_001564"
     CDS             3910..4410
                     /locus_tag="VGY24_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ4284526.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            4475..4840
                     /gene="rplL"
                     /locus_tag="VGY24_001565"
     CDS             4475..4840
                     /gene="rplL"
                     /locus_tag="VGY24_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ4284527.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
BASE COUNT         1508 a          935 c         1086 g         1469 t
ORIGIN      
        1 ttctagagtt aaacaaaaaa cgccatgcca ttaagacctt taatgaccag ccagttgact
       61 acgaggattt gcgaacagcc attgagattg caaccttagc acctagtgcc aataacattc
      121 aaccttggaa atttgtagtc gttcaagaga aaaaagctga gttagcaaaa gggttacctc
      181 tagcaaataa agtgcaggtt gaacaggctc aatatgtagt ggcgctattt tcagacactg
      241 atttagcttt acgttctcgt aaaattgccc gtatcggtgt caaatcgtta ccagacgact
      301 taattggtta ttatatggag actttgccac cacgttttgc tgcttttaat gaggttcaaa
      361 ccggtgagta cttagctatt aatgcaggca ttgtggctat gaacttggtg ttatccttga
      421 cggatcaaaa gattgcttcc aatattatcc ttggttttga taagtcaacg accaatgaaa
      481 ttttagacat cgatccgcgt tttcgtccag aattattgat tactgttggc tatagtgacg
      541 agaaaccaga accaagttat cgtttgcctg ttgatgaagt catcgaaaga agatagagtc
      601 aggacaatta gttaaatgta atgtccagat gcgctactga gcgaccacta ttgttagact
      661 gatactattt tggcttattt taagttaaac attatcccta aaacgaataa ggagagtata
      721 atgacaacca ttgattttaa agcagaagtt gacaaacgta aagaagctat gctagcagat
      781 ttagttgacc tattacgcat taattcagaa cgtgatgacc aattagctga tgataaacac
      841 ccatttggcc caggtccagt taaggccttg gagcatttcc tcgcaatggc tgagcgtgat
      901 ggctacaaaa cgcgtaatat tgataactat gcaggtgact ttgaatttgg tcaaggtgat
      961 gaagtactag gaatctttgg tcaccttgat gttgtgccag ctggtagcgg ttgggataca
     1021 gacccttatg agccagtgat taaaaacgat cgcatctatg cgcgtgggtc ttcggatgat
     1081 aagggaccaa ccatggcgtg ctactacgct ctgaaaatca ttaaagaatt gggtcttcct
     1141 gtgtctaaaa aagttcgttt cattgttggt accgatgaag aatctggctg gggggatatg
     1201 gattattact ttgctcataa tggcttgaaa aatctagatt ttggtttctc accagatgct
     1261 gaatttccaa ttatcaatgg tgagaaagga aacatcaccg aatacctcca tttcgctggt
     1321 gacaataatg gtgcttttgt gcttcatcgc ttccaaggtg ggttgcgtga aaacatggta
     1381 ccagaatctg caacagctgt cattacggca ccgcatgacc ttgatgtgct agaagcagcc
     1441 ttggagcaat ttttatcaga acatggtgtt aaaggttcta tgaaggctac tgatggtaaa
     1501 attgaagtaa ctattattgg gaagtctgct cacgggtcga ctccagaagc gggtgttaat
     1561 ggtgccacct tgttggctaa attcctgaat caatttacct ttgaaggggc tgctaaggat
     1621 tatttacatg tggcaggtga ggtgcttcat gaagactttg cggcagaaaa actaggcctt
     1681 gattataccg atgaccgtat gggagccctg agcatgaacg caggtgtctt cacctttgat
     1741 agtcaatcag ctgacaatac catcgccttg aatttccgtt atccaaaagg gactgatgcg
     1801 gcaaccttaa aagcaggatt ggaaaaatta ccaggattaa ctaaggtaag cctttctgag
     1861 catgaacata cacctcatta tgtgccaatg gatgatgaat tagtagctac cttacttgct
     1921 gtttacgaaa aacaaacagg tcttaaaggc tacgaacaag ttatcggcgg aggaaccttt
     1981 ggtcgtttgc tagaacgtgg tgtggccttt ggagcgatgt tcccaggcga tgaaaacact
     2041 atgcaccaag ctaatgaata tatgccttta gaaaatattt atcgctcagc agctatttat
     2101 gctgaagcaa tctacgaatt aattaaataa gatgcttatg acagtaaaaa gggctggaac
     2161 attgttccag ctcttttgtg atgattagaa aatggtctta tttaccaaga caaaattggc
     2221 taaagagttg ggtaatgagt tcatcaggag cagcatcccc tgtgatttct ccgagaattt
     2281 cccaagttct ggttaaatca acctgtagta aatcaactgg catgccaagc gccaagccgt
     2341 cattgacagc ttctaaactc tggactgcct tttcaatcaa agaaatgtgg cgggcatttg
     2401 acaggtaagt ggcatcttgt tctactagtc cagcattatc aaagaagagc tggttgatac
     2461 gatcttcgat cagattaatg ttctggttgg ttaacaccga aataggaata aggtccgctg
     2521 gcaactgttc caattcaatc ttttgttcca aatctgtttt attcagcaat ataatgcggt
     2581 tactgtcttg actgagattt aacaaagccc ggtcttggtc agtcagtttt tcagaagcat
     2641 tcagcacaag aagaacaagg tcagcttctt gaagagcttt tttagaccgt tcaactccga
     2701 tttgttcgac cagatcatct gtctcacgaa taccggccgt atcgacaagt tttagaggaa
     2761 ctcccttgat attaacgtat tcttcaatga catctcgggt ggttcctgca atatcagtca
     2821 caatagcctt gtcttctcgc aacaaattat taagtaagct tgacttaccg acgtttggac
     2881 gaccaataat agctgttgat aagccctcac gcagaatttt accgcgttta gctgttcgaa
     2941 ggagattttc caaaaggctt tggaattctt gtgttttctc acgtagcaga gcagtggtca
     3001 tttcttccac atcgtcgtat tcagggtagt caatattgac ttcgacttga gctaaggtgt
     3061 tgagaatttc ttgtcgggtg tcattgataa gctgggaaag ggagccatcc agttgcttga
     3121 ctgcaatcgt catagccttg tctgttttgg cacgaataat gtccatgaca gcctcagctt
     3181 gagtcaaatc aacacgacca tttaagaagg cacgtttggt gaattcgcca ggctctgcca
     3241 ttctagcgcc ttggcgaatc aatagttgca agatttcatt tgtcacagca ataccaccgt
     3301 gcgtattgat ttcaaccaca ttttcacggg taaaggtttt gggagccagc atgactgaca
     3361 ccataacctc atcgacaata gttcctgtgt taggatcaat gatatggcca tagttaatgg
     3421 tgtgtgaagc tacttgctct aagttctttc ccttaaaaac agattgtgca atggcaagcg
     3481 catctgtccc ggatagacga acaatgccaa tagctccttc accaagaggg gttgaaatgg
     3541 cggtaattgt atcaaattct ttagttatac tcatgccctc tattttaaat caagtcctag
     3601 tagaaagcaa ggtattattt tgtatagaaa ataggactag caccattttg agtttagcta
     3661 atactaaaaa agaataaatg taaaaaaatc atttttttaa taatttttat tgacaatagt
     3721 attataaaag aatataatga tataggtttt gataaaaaac tgacctaaga cagcagggga
     3781 gcatgctcat aatattcctg ccgaggcaca aaacgtgatt aaagagaata acgtatttgt
     3841 actttcgtgt ctaggtttag gtgcgatttt tttgtaccta gcccaaaaat aaaaacggag
     3901 gtaaaactaa tgagtgaagc aattattgct aaaaaagctg aacaagtcga acttattgct
     3961 gagaaaatga aagcagcagc aagtatcgtt gttgttgatt cacgtggtct tacagttgat
     4021 caagatactg ttcttcgtcg ttcacttcgt gaaagtggcg ttgagttcaa agttatcaaa
     4081 aactcaattt tgactcgtgc agctgaaaaa gcaggtcttg atgagttgaa agatgtattc
     4141 gtaggaccat ctgcagtagc attttctaac gaagatgtta tcgcaccagc taaagttatc
     4201 aacgacttta ctaaaactgc tgacgcactt gaaatcaaag gtggtgcaat cgaaggcgct
     4261 gtttcttcaa aagaagaaat ccaagcactt gcaacattgc caaaccgcga aggaatgctt
     4321 tctatgcttc tttctgtact tcaagctcca gtccgcaacg ttgcatacgc tgtcaaagct
     4381 gttgcagaaa acaaagaagg tgctgcttaa gacgtctcag agtagcttaa ggctacgctt
     4441 aacattttaa attaattatt tggaggaaat cacaatggca ttgaacattg aaaacattat
     4501 tgctgaaatt aaagaagctt caatccttga gcttaacgat cttgtaaaag ctatcgaaga
     4561 agaatttggt gtaactgcag ctgctcctgt agctgctgca gctgctggtg gtgctgaaga
     4621 agctgctaaa gattcattcg acgttgaatt gacatctgct ggcgacaaaa aagttggcgt
     4681 tatcaaagct gttcgtgaaa tcacaggtct tggtcttaaa gaagctaaag gtcttgttga
     4741 tggagcacct gctaacgtta aagaaggcgt tgctgctgca gaagctgaag aaatcaaagc
     4801 taaacttgaa gaagctggag caacaatcac tcttaaataa gaagcagata gcaatttgag
     4861 agcgaaatcc cgatttatca gttttttaga gcttatagcg atttgaagaa actgataaaa
     4921 tgatttggtt tcttgccaaa aatcctgcca aaaatttttt ggcaggattt ttattttcta
     4981 taaaaattaa gctgagta
//
LOCUS       DAUCWU010000099         4843 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUCWU010000099 DAUCWU010000000
VERSION     DAUCWU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.099"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            39..554
                     /gene="lepB"
                     /locus_tag="VGY24_001566"
     CDS             39..554
                     /gene="lepB"
                     /locus_tag="VGY24_001566"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ4284528.1"
                     /translation="MVIIGAILLRIFVFSTFKVSPETANTYLKSGDLVTIKKNIQPKY
                     KDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYLEKMKAHYLNHAPFG
                     TLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLINASQIKGLVTFRVL
                     PLSDFGFVEVE"
     gene            730..2544
                     /gene="glmS"
                     /locus_tag="VGY24_001567"
     CDS             730..2544
                     /gene="glmS"
                     /locus_tag="VGY24_001567"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ4284529.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2726..3061
                     /locus_tag="VGY24_001568"
     CDS             2726..3061
                     /locus_tag="VGY24_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ4284530.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            3168..3809
                     /locus_tag="VGY24_001569"
     CDS             3168..3809
                     /locus_tag="VGY24_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ4284531.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFITGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3819..4448
                     /locus_tag="VGY24_001570"
     CDS             3819..4448
                     /locus_tag="VGY24_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284532.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKAFDLLTRLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            4464..>4843
                     /locus_tag="VGY24_001571"
     CDS             4464..>4843
                     /locus_tag="VGY24_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4284533.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKGLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQEKVAFTDSYMR"
BASE COUNT         1474 a          793 c         1037 g         1539 t
ORIGIN      
        1 taaagcgaga ttttataaga aatatattat tgttattaat tgtgattatt ggggctattt
       61 tgttgagaat ctttgttttt tcaactttta aagtttctcc agaaacagct aatacttatt
      121 taaagagtgg tgatttagtc acaatcaaaa aaaatattca gcccaaatat aaagattttg
      181 tagtttatag agttggaaaa aaagattatg tcagtcgagt cattgctgtt gaaggcgata
      241 gcgtgactta tatggacgat attttttacc tcaataatat ggtagagtca caggcttacc
      301 ttgaaaagat gaaagcacat tacttgaatc atgcaccgtt tggcacattg tatacagatg
      361 attttacagt tgctaccatc acagctgata agtatcaaaa agttcctaag gggaagtatc
      421 ttcttttgaa tgataaccgg aaaaatacga atgacagtcg tcgatttggg ttaataaatg
      481 cctcgcagat taaaggttta gtgaccttta gagttttgcc tctcagcgat tttggatttg
      541 tagaagtaga gtagttgtca ggctactcta ctttttttat aattagacta taccaatttt
      601 atgtttgaca agacattgtg gatattatat actgtaaaca tactgttttt atcggtataa
      661 tatgactata ccaattattg aaattatgag agaaagtagc aagttaacga acttgttaag
      721 gtaattaaga tgtgtggaat tgttggagtt gttggaaatc gcaatgcaac ggatatttta
      781 atgcaaggcc ttgaaaagct tgaataccgg ggttatgatt cagcaggaat ttttgtggct
      841 aatgccaatc aaacaaactt gattaaatca gtggggcgga ttgctgattt gcgtgccaag
      901 attggcattg atgttgctgg ttcaacaggg attggtcaca cccgttgggc aacgcatggc
      961 caatcaacag aggataatgc ccatcctcac acgtcacaaa ctggacgttt tgtacttgtt
     1021 cataatggtg tgattgaaaa ttaccttcac attaaaacag agttcctagc tggacatgat
     1081 tttaaggggc agacagatac tgagattgca gtacacttga ttggaaaatt tgtggaagaa
     1141 gacaagttgt cagtactgga agcttttaaa aaagctttaa gcattattga aggttcctac
     1201 gcctttgcgt taatggatag ccaagcaact gatactattt atgtggctaa aaacaagtct
     1261 ccattgttga ttggacttgg tgaaggttac aacatggttt gttcagatgc catggccatg
     1321 attcgtgaaa ccagtgaatt tatggaaatt catgataagg agctagttat tttaaccaaa
     1381 gataaggtaa ctgttacaga ctacgatggt aaagagctga tacgagattc ctacactgct
     1441 gaattagact tatctgatat tggcaaaggg acttatcctt tctatatgct gaaagaaatt
     1501 gatgagcaac caaccgtaat gcgtcaatta atttcaactt atgcagatga aactggtaac
     1561 gtacaggttg atccagctat cattacctct atccaagagg ctgaccgtct ttatatttta
     1621 gcggcaggga cttcctacca tgctggtttt gcaacaaaaa atatgcttga gcaattgaca
     1681 gatacaccag ttgagttggg agtggcttct gagtggggtt accacatgcc tctgcttagc
     1741 aagaaaccaa tgtttattct actaagccaa tcaggagaaa ccgcagatag tcgtcaagtt
     1801 ttagtaaagg caaatgctat gggcattccg agtttgacag taactaacgt tccaggatca
     1861 accttatcac gtgaagcaac atacaccatg ttgattcatg ctggacctga aattgctgtt
     1921 gcgtctacaa aagcttacac tgcacaaatt gctgcccttg cctttttggc taaggcagtt
     1981 ggtgaggcaa atggtaagca agaagctctt gactttaact tggtacatga gttgtcattg
     2041 gttgcccaat ctattgaggc gactttgtct gaaaaagatc tcgtggcaga aaaggttcaa
     2101 gctttgctag ctactactcg taatgctttt tacatcgggc gtggcaatga ttattacgtt
     2161 gcgatggaag ctgctttgaa attaaaagag atttcttata ttcaatgcga aggctttgcg
     2221 gctggtgaat tgaaacatgg aaccatttca ttaattgagg aggacacgcc agtaatcgct
     2281 ttaatatcgt ctagtcagtt ggttgcctct catacgcgtg gtaatattca agaagttgct
     2341 gcccgtgggg ctcatgtttt aacagttgtg gaagaagggc ttgaccgtga gggagatgac
     2401 attattgtca ataaggttca tcctttccta gccccgattg ctatggtcat tccaactcaa
     2461 ctgattgctt actacgcttc attacaacgt ggacttgatg ttgataagcc acgtaatttg
     2521 gctaaagctg taacagtaga ataaatgatt ataatggata aggcaaccat ctgttaggtg
     2581 gttgcttttt gtttttagga gaatagcaga gcttagaaaa tggccttgag aagaatagtc
     2641 atcttctgat gagaacgtat tcatcttgtc aaatcattga ttctaaagta aaatggtaac
     2701 attaagaaat gtttaggagt gtaagatgtc attaccaaat tgtttgcaat gccagtctga
     2761 atatgtttac gaagatggtc ttttattaat ctgtccaatg tgtgcttttg aatggacacc
     2821 aggcgaagaa gcgactgaag aagaaggact tgttgttttg gatagcaatg gtgtccgact
     2881 gtctgatggt gataccatta ctgtggttaa agatttgaaa gtaaaaggcg ctccaaaaga
     2941 cctgaaacaa gggactcgtg ttaaaaatat tcgcctggtg gaaggtgatc ataacattga
     3001 ttgtaagatt gatgggtttg gcgcgatgaa attgaaatca gaatttgtca agaaaatcta
     3061 ataataatac gcaaccagct gatctggttg tatttttata aaaaaacctc tccccaaata
     3121 cgttcaaatt tggtataata gtaaaatatt cagttttagg aggcaacatg acctatattc
     3181 agcaagtttt acccagctta ttagatggtg ccttggtgac cttacaagta ttctttattg
     3241 ttatcattct ttctataccc ttaggggcta ttttagcttt cttgatgaag attcccttta
     3301 aaccgctaca gtggtttttg accctatacg tgtggatgat gcgagggaca ccattactac
     3361 ttcaattgat ttttttctat tatgttttgc caagtgtggg gattagtttt gatcgaatgc
     3421 cagctgctat tttggcgttt actttgaatt atgctgccta ctttgctgaa atttttagag
     3481 gtgggattga ggctattcca aaaggtcaat atgaagcagc taaagtatta aagttaaaac
     3541 ctcttcaaac cattcggtat attattttgc ctcaagtgtt taaaattgtg ttaccaagtg
     3601 ttttcaatga agtcattaat ttggtcaaag attcttccct tgtctatgta ctcggtgtag
     3661 gagatctttt attagcaagt aagacggcag ccaataggga tgcaacctta gcccctatgt
     3721 ttatcactgg tcttatctat ttgcttttaa ttggactggt cacgattatt tcaaaacaag
     3781 ttgaaaaacg gtttaattat tatcagtaag gaggcttcat gttagaactc aaaaatattt
     3841 ccaaacagtt tggtcaaaaa accatttttg atggctttaa tctaaccgtt caagacggag
     3901 aagtgctctc tttggtaggt ccatcaggtg gcggtaagac aacactctta cggatgttgg
     3961 ctggtcttga atctattgat tcaggtcaag tcttttacaa tggagaagat gtcggtattg
     4021 atcatttgga aaatcgtaac ttgcttggat ttgttttcca agattttcaa ttgtttcctc
     4081 atcttaccgt tttagacaac ctaacgctgt ctccaacaat tacaatgggc aagcaaaagg
     4141 ctgacgctaa agaaaaggct tttgatttac tgacacgttt aggattaaaa gaacatgccc
     4201 aagtttatcc ctattcgtta tctggaggtc aaaaacaacg ggttgctttg gcgagagcca
     4261 tgatgattga tcctcaaatt atcggttacg atgaaccgac cagtgcccta gatcctgaat
     4321 tacgtcagac ggtagaagcc ttgattgttc aaaaccgtga aatgggaata actcaaattg
     4381 tagtcactca cgatcttgtt tttgccgaag ccatctcaga tcgtattatt agggttaatc
     4441 ctaaatagaa gaggacccaa aagatgatta taaaaaaaag aaccgtagca attttagcca
     4501 tagctagtag ctttttcttg gtagcttgtc aagctactaa aggtcttaaa tcaggagatg
     4561 cttggggagt ttaccaaaag caaaaaagta ttacagttgg ttttgacaat acgtttgttc
     4621 ctatgggcta taaggatgaa agcggcaaat gcaaaggttt tgatattgat ttggctaaag
     4681 aagtttttca ccaatatgga ctcaaggtta actttcaagc tattaattgg gacatgaaag
     4741 aagcagaact aaacaatggt aaaattgatg taatctggaa tggttattca ataactaagg
     4801 agcgtcagga aaaggttgcc tttactgatt cttacatgag aaa
//
LOCUS       DAUCWU010000100         4697 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUCWU010000100 DAUCWU010000000
VERSION     DAUCWU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.100"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            292..819
                     /locus_tag="VGY24_001572"
     CDS             292..819
                     /locus_tag="VGY24_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ4284534.1"
                     /translation="MTNTLVENIYASVTHNISKKEASNNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            961..1164
                     /locus_tag="VGY24_001573"
     CDS             961..1164
                     /locus_tag="VGY24_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ4284535.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLN
                     KRDYLNAELILRREHRLELEKEG"
     gene            1169..2140
                     /locus_tag="VGY24_001574"
     CDS             1169..2140
                     /locus_tag="VGY24_001574"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ4284536.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGVIADGNLIHGVTGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            2150..2539
                     /locus_tag="VGY24_001575"
     CDS             2150..2539
                     /locus_tag="VGY24_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ4284537.1"
                     /translation="MSPITLILWLLLVGIVGYHTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRTKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            2722..4563
                     /gene="typA"
                     /locus_tag="VGY24_001576"
     CDS             2722..4563
                     /gene="typA"
                     /locus_tag="VGY24_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ4284538.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDSSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
BASE COUNT         1461 a          842 c         1018 g         1376 t
ORIGIN      
        1 gtagtaaaca gtaaaaaaga tgggtcaata agatgcccga acatatacta aaaaaagcta
       61 tcatacttat ttattcgaaa agaaatcaaa tacttgcaat ctttccttaa tcattgtaga
      121 atatagatat aaaacgataa tgattctaat taagtatcta atcatccaat taggattatt
      181 ccgttattgt cgaataggat taggtcctta accctttctt atgtttatca cgttagggtt
      241 agttccccct ccattcttgc cctttaagct gaaaagtaag gagatgttat tatgacaaac
      301 acactcgttg aaaatattta tgcttcagta actcacaata tttctaaaaa agaagcatca
      361 aacaatgaaa aaactaaagc tgttttaaac caagctgtcg ctgacttatc tgtggctgca
      421 tctattgtgc accaagtaca ctggtatatg cgtggtccag gtttcttgta ccttcacccc
      481 aaaatggatg aattacttga tagcttgaat gctaatttag atgaaatgag cgagcgtttg
      541 atcactattg gcggagcgcc ttattctacc ctagctgagt tttctaaaca ctctaaattg
      601 gacgaagcaa aaggtactta cgataaaaca gttgctcagc atttagcaag attagtcgaa
      661 gtttaccttt acctcagcag cctttaccaa gttggtttgg atattaccga tgaagaaggc
      721 gatgctggaa caaacgattt gtttacagca gctaagacag aggctgaaaa aaccatttgg
      781 atgctccaag cagagcgtgg acaaggccca gcactctaat ggattatgcc ttaggataaa
      841 agaagtcaat gataaaattg gcttcttttt tatggaatca tcgattcatt ttcagaaaca
      901 tagaaagcga ttccatactt gtcttgaaat ctgccgtaga atttgctaaa atctacttat
      961 atgaaaacac tatatgatgt gcaacaattg ctaaaaaact ttggcatttt tgtttacctt
     1021 ggcaaacgtc tttatgatat tgaaatgatg aagattgaac ttcagcgtct ctatgatagc
     1081 ggcttattga ataagaggga ttacttaaat gcggagttaa ttttgcgacg tgagcataga
     1141 ttagaattgg aaaaagaagg atagacaaat gagtcaaaaa ttactgggga ttgatttagg
     1201 tggtaccacc attaaatttg gaattttaac agcagcagga gaagtccaag aaaaatgggc
     1261 aattgagact aatattttag aaggaggcaa acatattgtc cctgatatca tcgcttccat
     1321 caaacatcgc ttagacttgt atggtctaag cagtgctgac tttgtgggaa ttggtatggg
     1381 atcaccagga gcggttgacc gtgatactaa tacagttact ggggcattca accttaattg
     1441 gaaagaaacc caagaagtcg gttccgttgt tgaaaaagaa ttgggcattc cttttgccat
     1501 tgacaatgat gccaatgtgg ctgcccttgg tgaacgttgg gtaggtgctg gtgaaaataa
     1561 cccagatgtc gtcttcatga cacttggaac aggtgtcggt ggaggcgtta ttgctgatgg
     1621 taacttgatt catggtgtta caggagcagg tggtgaaatc ggccacatga ttgttgagcc
     1681 agaaaatggc tttgcttgta cttgtggctc acacggctgt ttggaaacag tagcttcagc
     1741 aacaggagtt gtcaaagtgg cacgtttact ggcagaagcc tacgaagggg attcagccat
     1801 caaagcagct attgacaatg gtgaaggtgt caccagcaaa gacattttca tggcggctga
     1861 agcaggggat tcctttgctg attctgttgt ggaaaaggtt ggttactacc ttggccttgc
     1921 ttcagcaaat atttccaata tcttgaatcc agactcagtg gttatcggtg ggggtgtgtc
     1981 agcagcagga gaatttctac gctcacgtat tgaaaaatac tttgtgacct tcactttccc
     2041 acaagtgagg tattcaacta aaattaagat tgcagagctt ggcaatgatg ctggaattat
     2101 cggtgcagct agccttgctc gacaatttat cacaaaatag gagggggata tgtctccaat
     2161 cacacttatc ttatggttac ttcttgtcgg cattgttggt tatcatacat ggaactattt
     2221 ctcttttcga aaaatggcaa agcaggttga taacgagact tttaaagacg tgatgcgtca
     2281 aggtcaattg attgatttac gagagccagc ggcttttaga actaagcata ttttaggagc
     2341 tcgaaatttt ccagcgcaac aatttgatgc tgctatcaaa ggtcttcgca aggacaaacc
     2401 tgttcttatt tacgaaaata tgcgtcccca gtaccgtgtg cctgcggtca aaaaacttaa
     2461 aaaagctggc tttgaagacg tttatgtctt aaaagacggt attgattatt gggatggcaa
     2521 agttaaacaa acaacctagt taagagtaac gtggagttaa ggggaataaa ggcagtcact
     2581 gtctcaaaaa ccttaattcc tttttttgct gtatccagac ttgctgaaag tctgaaaaca
     2641 tttacaattg attaaaacca gttttttaaa acattttgtg ttatacttat ctagttaaaa
     2701 tatatttact tagaggaaca aatgactaac ttaagaaacg atatccgtaa cgtagcgatt
     2761 attgcccacg ttgaccacgg aaaaacaaca cttgtagatg aattattaaa acaatcccat
     2821 actcttgatg agcgtaaaga gcttcaagag cgtgccatgg attccaatga ccttgaaaaa
     2881 gaacgtggga ttacaatcct tgcgaaaaat acggcagtag cctataacga tgttcgtatt
     2941 aacatcatgg ataccccagg acacgcggac ttcggtggtg aagttgaacg tatcatgaaa
     3001 atggttgacg gggttgttct tgttgtggat gcctacgaag gaacaatgcc ccagacgcgt
     3061 ttcgtattga aaaaagcact tgagcaaaac cttatcccga tcgttgtggt gaacaagatt
     3121 gacaaacctt cagctcgtcc agcagaagtt gtagatgaag tgcttgaatt attcatcgaa
     3181 cttggtgccg atgatgagca attggaattc ccagttgttt acgcatcagc tattaatgga
     3241 acatcatcat tatcagatga ccctgctgac caagagcata ctatggcacc gatctttgat
     3301 acgattattg atcatattcc agcgccagtt gatagttcag atgagccttt gcaattccaa
     3361 gtgtcacttt tggactacaa cgatttcgta ggtcgtatcg gtatcggtcg tgttttccgt
     3421 ggtactgtta aagtgggtga ccaagtaact ctttcaaaac ttgatggtac cactaaaaac
     3481 ttccgtgtta caaaactgtt tggtttcttc ggtttggaac gtcgtgaaat tcaagaagct
     3541 aaagcaggtg acttgattgc tgtttcaggt atggaagata tctttgttgg agaaaccatt
     3601 acaccaactg actgtgtgga agctctgcca attcttcgta ttgatgagcc aacacttcag
     3661 atgactttct tggtcaataa ctctcctttt gcaggtcgtg aaggtaaatg gatcacgtca
     3721 cgtaaggttg aagaacgtct tttagcagaa ttgcaaacag acgtgtcact tcgtgttgac
     3781 ccaacagatt cgccagataa atggacggtt tcagggcgtg gagaattgca tttatctatc
     3841 ctcattgaaa ccatgcgccg tgaaggctat gaacttcaag tatcacgtcc agaagttatc
     3901 atcaaagaaa ttgatggtgt caaatgtgaa ccgtttgagc gtgttcaaat tgatacacca
     3961 gaagaatatc agggtgcaat catccagtct ctttcagaac gtaaagggga tatgcttgat
     4021 atgcagatgg ttggtaatgg tcaaacgcgt ttgattttct tgattcctgc acgtggtttg
     4081 attggttatt caacagagtt tctttcaatg acacgtggat atggtatcat gaatcatact
     4141 tttgatcagt atctaccggt tgttcaagga gaaatcggtg gccgtcatcg tggtgccttg
     4201 gtttctattg aaaatgggaa agcaacgaca tattcaatca tgcgtattga agaacgtgga
     4261 actatctttg tcaacccagg tacagaagtt tacgaaggga tgattgttgg tgaaaactca
     4321 cgtgataacg accttggtgt taacattaca acagctaagc aaatgacaaa tgtccgttca
     4381 gcgactaaag atcaaactgc agttatcaag acgcctcgta ttctaacgct tgaagaatca
     4441 ttagagttct tgaatgacga cgaatacatg gaagtaacgc cagaatctat ccgcttgcgt
     4501 aaacaaatct tgaataaagc tgcgcgtgat aaagctaata aaaagaaaaa atcagccgaa
     4561 tagcttttga agttaaacta actaaataaa atagcaaaaa gatatttaag aaaagtcatt
     4621 ttttaaaggt ctttttgcgt ttacaagttc ctatggaata taaaccaaaa taaaggagga
     4681 cggaatgttt tacctta
//
LOCUS       DAUCWU010000101         4654 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUCWU010000101 DAUCWU010000000
VERSION     DAUCWU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.101"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            108..380
                     /gene="rpsP"
                     /locus_tag="VGY24_001577"
     CDS             108..380
                     /gene="rpsP"
                     /locus_tag="VGY24_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ4284539.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            390..629
                     /locus_tag="VGY24_001578"
     CDS             390..629
                     /locus_tag="VGY24_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ4284540.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
     gene            1561..4587
                     /locus_tag="VGY24_001579"
     CDS             1561..4587
                     /locus_tag="VGY24_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ4284541.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKTVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
BASE COUNT         1629 a          734 c          872 g         1419 t
ORIGIN      
        1 agaaaaaaac ttgacacttt ctttaaactg agttatactg tttaacatag gaatttagcg
       61 tagctgtatt tcttatacat tactaaaaga aaagagacta tttaaaaatg gcagtaaaaa
      121 tccgtttaac tcgtatgggt tctaagaaaa aacctttcta ccgtattaat gttgcagatt
      181 cacgtgcacc acgtgatggt cgttttatcg aaacagttgg aacatacaac ccattagtgg
      241 ctgaaaatca aattactatt aaagaggatc gtgttcttga gtggttatct aaaggagcgc
      301 agccttctga taccgttcgt aacatcttgt ctaaagctgg tgttatggct aagttccacg
      361 accaaaaatt ctctaaataa taacagccta tggataccat tgaaaatctt attatcgcta
      421 ttgtgaaacc tttgatttca caaccagata atcttaccat taaaattgaa gatactcctg
      481 acttcttaga gtatcatctt gacttggatg ctcaagacat cggtcgtgtt atcggcaaaa
      541 aaggtcgaac tattactgcg ataagatcga ttgtctactc ggttccaaca ctaggaaaaa
      601 aagttcgctt agttattgat gagaaataat cactaacttg agatggattg aaaagcttgt
      661 tgactttaag tcaacaagct ttttgaatag caaaaatctt aatatttctg aaatgaaaag
      721 attagatttt tctgtcttta actaaaaaga agttaaagaa tggcttagct gattgccttt
      781 ataagagatt atactggttt ttgatagctg ctgtcatgta aaatagctac aattcatctt
      841 acgagattga ccttagcatt gccagtgcag tctttatttt ccaacctgct gtttgttgta
      901 tgtttatagt ttcctaaaaa atgctctctc agttttttgg gggacattcc taaaattaag
      961 tatacttaat tttaggaatg tttatattct ttacattctg atgaaaatac aaattatcct
     1021 aacatgatgt attgaaaggg gaaatagttt atgttagtat actgattaag tgaagctgta
     1081 atgaggctta tacgatcaaa aaagttagag taactttata aaacagtggt gttgaagcaa
     1141 gtttagcttc ttctcagaaa tagattatct ataaaaaatc ccaaaagtag tttgtttcca
     1201 gttaatgaac ttcttttggg aaggcttttt tgctgatcgc cacttcactt gtatatcggg
     1261 aacattcacg ctaagatgta taagttgaat cgtttgtcta tggcttgatg aaaattaatc
     1321 aaaagataat gttcaactct ataattagta caagcaaaat gagataactt attcattagt
     1381 aagcataaga tataacaaga gaaattacct taaaacagga agtactagag tatttaaaat
     1441 aagtgataga cttggatatt attaggtcga ctagtgtcct tttcaataat ttaatatcgt
     1501 tattattagt atcagccaat caaatgtgga taagttggct tttagaaagg ttttaccaca
     1561 atgaagaaac atcttaaaac agttgccttg accctcacta cagtatcggt agtcacccac
     1621 aatcaggaag tttttagttt agtcaaagag ccgattctta aacaaactca agcttcttca
     1681 tcgatttctg gcgctgacta cgcagaaagt agcggtaaaa gcaagttaaa gattaatgaa
     1741 acttctggcc ctgttgatga tacagtcact gacttatttt cggataaacg tactactcct
     1801 gaaaaaataa aagataatct tgctaaaggt ccgagagaac aagagttaaa gacagtaaca
     1861 gagaatacag aatcagaaaa gcagattact tctggatctc aactagaaca atcaaaagag
     1921 tctctttctt taaataaaac agtgccatca acgtctaatt gggagatttg tgattttatt
     1981 actaagggga atacccttgt tggtctttca aaatcaggtg ttgaaaagtt atctcaaact
     2041 gatcatctcg tattgcctag tcaagcagca gatggaactc aattgataca agtagctagc
     2101 tttgctttta ctccagataa aaagacggca attgcagaat ataccagtag ggctggagaa
     2161 aatggggaaa taagccaact agatgtggat ggaaaagaaa ttattaacga aggtgaggtt
     2221 tttaattctt atctactaaa gaaggtaaca atcccaactg gttataaaca tattggtcaa
     2281 gatgcttttg tggacaataa gaatattgct gaggttaatc ttcctgaaag cctcgagact
     2341 atttctgact atgcttttgc tcacctagct ttgaaacaga tcgatttgcc agataattta
     2401 aaagcgattg gagaattagc tttttttgat aatcaaatta caggtaaact ttctttgcca
     2461 cgtcagttaa tgcgattagc agaacgtgct tttaaatcaa accatatcaa aacaattgag
     2521 tttagaggaa atagtctaaa agtgataggg gaagctagtt ttcaagataa tgatctgagt
     2581 caactaatgc tacctgacgg tcttgaaaaa atagaatcag aagcttttac aggaaatcca
     2641 ggagatgatc actacaataa ccgtgttgtt ttgtggacaa aatctggaaa aaatccttat
     2701 ggtcttgcta ctgaaaatac ctatgttaat cctgataagt cactatggca ggaaagtcct
     2761 gagattgatt atactaaatg gttagaggaa gattttacct atcaaaaaaa tagtgttaca
     2821 ggtttttcaa ataaaggctt acaaaaagta aaacgtaata aaaacttaga aattccaaaa
     2881 cagcacaatg gtgttactat tactgaaatt ggtgataatg cttttcgcaa tgttgatttt
     2941 caaaataaaa ctttacgtaa atatgatttg gaagaagtaa agcttccctc aactattcgg
     3001 aaaataggtg cttttgcttt tcaatctaat aacttgaaat cttttgaagc aagtgacgat
     3061 ttagaagaga ttaaagaggg agcctttatg aataatcgta ttgaaacctt ggaattaaaa
     3121 gataaattag ttactattgg tgatgcggct ttccatatta atcatattta tgccattgtt
     3181 cttccagaat ctgtacaaga aatagggcgt tcagcatttc ggcaaaatgg tgcaaataat
     3241 cttattttta tgggaagtaa ggttaagacc ttaggtgaga tggcattttt atcaaataga
     3301 cttgaacatc tggatctttc tgagcaaaaa cagttaacag agattcctgt tcaagccttt
     3361 tcagacaatg ccttgaaaga agtattatta ccagcatcac tgaaaacgat tcgagaagaa
     3421 gccttcaaaa agaatcattt aaaacaactg gaagtggcat ctgccttgtc ccatattgct
     3481 tttaatgctt tagatgataa tgatggtgat gaacaatttg acaataaagt ggttgttaaa
     3541 acgcatcata attcctacgc actagcagat ggtgagcatt ttatcgttga tccagataag
     3601 ttatcttcta caatggtaga cctcgaaaag attttaaaac taatcgaagg tttagattat
     3661 tctacattac gtcagactac tcaaactcag tttagagaca tgactactgc aggtaaagcg
     3721 ttgttgtcaa aatctaacct ccgacaagga gaaaaacaaa aattccttca agaagcacaa
     3781 tttttccttg gccgcgttga tttggataaa gccatagcta aagctgagaa ggctttagtg
     3841 accaagaagg caacaaagaa tggtcagttg cttgaaagga gtattaacaa agcggtatta
     3901 gcttataata atagcgctat taaaaaagct aatgttaagc gcctggaaaa agagttagac
     3961 ttgctaacag gattagttga gggaaaagga ccattagcgc aagctacaat ggtacaagga
     4021 gtttatttat taaagacgcc tttgccattg ccagagtatt atatcggatt gaacgtttat
     4081 tttgacaagt ctggaaaatt gatttatgca cttgatatga gtgatactat tggcgaggga
     4141 caaaaagacg cttatggtaa tcctatatta aatgttgacg aggataatga aggttatcat
     4201 gccttggcag ttgccacttt agctgattat gaggggctcg acatcaaaac aattttaaat
     4261 agtaagctta gtcaattaac atctattcgt caggtaccga ctgcagccta tcatagagcc
     4321 ggtattttcc aagctatcca aaatgcagcg gcagaagcag aacagttatt gcctaaacca
     4381 ggtacgcact ctgagaagtc aagctcaagt gaatctgcta actctaaaga tagaggattg
     4441 caatcaaacc caaaaacgaa tagaggacga cactctgcaa tattgcctag gacagggtca
     4501 aaaggcagct ttgtctatgg aatcttaggt tacactagcg ttgctttact gtcactaata
     4561 actgctataa aaaagaaaaa atattaaatt gatttaagga gaaaaaatga taacacaaga
     4621 aatgaaagat cttattaata accaattagc aatg
//
LOCUS       DAUCWU010000102         4597 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUCWU010000102 DAUCWU010000000
VERSION     DAUCWU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.102"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..532)
                     /locus_tag="VGY24_001580"
     CDS             complement(<1..532)
                     /locus_tag="VGY24_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ4284542.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLASMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLIT"
     gene            complement(615..2234)
                     /locus_tag="VGY24_001581"
     CDS             complement(615..2234)
                     /locus_tag="VGY24_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284543.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(2313..3185)
                     /locus_tag="VGY24_001582"
     CDS             complement(2313..3185)
                     /locus_tag="VGY24_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ4284544.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    3308..3549
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3604..>4597
                     /gene="trpS"
                     /locus_tag="VGY24_001583"
     CDS             3604..>4597
                     /gene="trpS"
                     /locus_tag="VGY24_001583"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ4284545.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEV"
BASE COUNT         1567 a          805 c          834 g         1391 t
ORIGIN      
        1 ctgtgataag tttatttaat ccaagtacaa caagtggaag aagaatgaaa acatctaacc
       61 aagaatttag ttccatttga cttgtcaaga agctcattaa gctataaaaa actgagatgg
      121 aaatcatcaa gaaagcactg atttttggat ataatctatg aaaagaatag catgcagcta
      181 atcctattaa cccaaatttt atcaaggtaa acaaatagat agcatctggc atagaggcta
      241 aattaaaaaa gaaaagtaat ggagaaaaga aactgccaag ataataacac attaaagcat
      301 aaaaatttat ccctagtccg cttgtaaagg tataaaaaaa actatcagaa ccgtgcatga
      361 tgttacgaaa gttctgcgca aaaataacat actgatgaaa agcatcacta gctagaattg
      421 ttttatcact attataataa atgcccatcg atagaaggat gataaatata atactaagag
      481 ggaacaaaaa actagcaagt ccagcaatta tccatttatt attatttttc ataaattata
      541 tggtctttcg tttacaaatt ttaaaaaaga ggctaggaag agatcttctt agccacctca
      601 tttatcataa agttttattt ccaaagttca gcaactcggg cttggacttc tggattgtca
      661 aggaattcgt cataagtttc atctatacgg tcgataacac cattttttga aataacaacg
      721 atgtggttag caatcgtttg aataaattca tggtcatgac tggcaaagat aacagattct
      781 ttgaagtctt tgatcccatc attaagactt gaaatagatt ccaaatccaa gtgatttgtt
      841 ggatcatcaa gaataagaac gtttgatttc aatagcatca acttagaaag catgacacgc
      901 actttttcgc caccggaaag aacgttaaca gattttttaa cttcgtctcc agagaaaagc
      961 atccgtccta ggaaaccacg taaaaaagta tcgtcatctt caccttttga ggcaaattga
     1021 cgtaaccatt caagaataga ttcttccgtg gcaaaatctt ttgaattgtc tttaggcaag
     1081 taagaacgac ttgttgtcac tccccattta atggttcctt cgtagtcaat atcatctgct
     1141 aaagcacgca taagagcggt tgtttgaata tcattttgac caataatagc agctttgtca
     1201 cctggacgta aaatgaaact gatattatca atgattttct ctccatcgat cgttacagaa
     1261 agattttcaa cagtgaggaa gtcattcccc atttcacgct ctgctttaaa attaataaat
     1321 gggtatttac ggcttgaggg aacaatttct tctaattcaa tcttatcaag cattttttta
     1381 cgagaggttg cttgttttga tttagatgcg tttgctgaga aacgagcaac gaattcttgt
     1441 aattctttga ttttttcttc ggctttagca ttgcggtcag cttgtaaacg agcggctagc
     1501 tcagaagatt gtttccagaa atcgtagtta cccacaaaga gtttgatttt accaaagtca
     1561 aggtcggcca tgtgagtaca tactttattc aagaagtgac ggtcatggga aacaacgatg
     1621 actgtatttt caaaatcaat caagaaatct tccaaccatg aaatagattg aatatcaaga
     1681 ccattggttg gctcgtcaag taataaaacg tcaggcttac caaagagcgc tttagcaaga
     1741 aggactttca ctttatctcc attagccagt tcactcatat tttggtagtg aaggtcttct
     1801 ggaatattta gattttggag aagttgagaa gcttcacttt ctgcctccca gccacctaat
     1861 tcagcaaaga taccttctag ctcagcggca cgaaccccat cttcttctga aaaatctgct
     1921 ttcatataga tagcgtcttt ttctttcatg atattgtaaa gttgctcatt acccatgatt
     1981 acaacatcaa tcgcacgttc ttcttcataa tcaaaatgat tttggcgaag aacagataag
     2041 cgttcgtcag gtcctaaaga gatatgacca gtacttggtt caatatctcc tgcaaggatt
     2101 ttaagaaaag ttgattttcc ggcaccattc gcaccgatta agccgtaagt attgccggct
     2161 gtgaatttga tattaacatc atcaaatagt tttcgatcac taaagcgtag tgatacatca
     2221 gaaactgtaa gcaatttgtc acctcatttt atttatctaa gcttcattgt acctaaaata
     2281 gtcccctttt tcaatcagaa aagggctaat cactacatag gcaataaaac gtctttgtct
     2341 tcacgtttgt attgtttagt caaactaaat cctcttccca taacttgagc tgctggcatg
     2401 acaacaataa aggctgtgtc gtcaatttcg aggatttttt cttggagagt gggtaattcg
     2461 tgacttgata caatagccat taacataatt ttatcagaag tagtataacc accgcgaata
     2521 ggaagtttag tcactccacg atccataaca gttgtgatat attctcgaat agcttgataa
     2581 tctttggaaa taatcataac atttttggat gagtcaaagc cattttccat aacgctaatc
     2641 acataaccaa ttacgaaaag tccaatcgta gaatacatca cgtcatctgc agaaagtgca
     2701 ataaatccca taaggacact aataccatct acaattgtca ttgcaactcc aagagataaa
     2761 ggagagtatt tatgaagtat ttgtgttaga attccagttc ctcctgtcga agagtttccc
     2821 caaaaaacca ttcctaaacc aatgccacag ataataccac caaaaatagc tgctaacaac
     2881 tgattgtgtg tcagagtagg taatgaattg gttgaacgga tggcaatagg atatatccaa
     2941 gaaccataaa gtgttttaat aaaattttgt tttccaagaa agaagtaaca cattaataat
     3001 aagggaatat tactagccat taaaaaaagc gacggactaa tgccaaatag agcttttata
     3061 acaactgcaa taccaaccat accccctgat gcaatatgat tgtcgacaaa cattgtattg
     3121 aaaccaatag ctgttatgaa tgagccaatt gtgacaagta gtaaatcaat ccctcgtttt
     3181 ttcatgcttt taatctcctt tataactggt taaaaatagt agactcatta tctcttaaat
     3241 gtactgagac cgtcaagtca tattttcaaa aaatctggtg aacttgacaa gaagtttaaa
     3301 atttcataga ataaatctaa attagaagag tattcttttc atgtttttag agagttagtg
     3361 gttggtgcaa gctaacaaca gaaaaggtga aatggactaa tgattatttg aaaggaaacc
     3421 ataactttca ggttggcacc ccttaccgtg cagcttcttt cattgagaag attgagatag
     3481 tatctcgttt tgataaagca gatgctaatt gaggtggtac cgcgtattac ttgtaataac
     3541 gccctcacgt tttaatagcg tggggacttt ttgctatatc aagagatagt tagggagaaa
     3601 atgatgacaa aacctattat tttaactgga gatagaccaa ctggaaaatt acatttaggt
     3661 cattatgtcg gaagtcttaa aaatcgtgtc tttttacaaa atgaaaacaa gtataagatg
     3721 tttgttttct tggccgatca acaggcactg acggatcatg ctaaagaatc cgaattaatt
     3781 caagaatcta ttggaaatgt tgccttggat tacctctctg tcggtttgga tccgaagcaa
     3841 tcaacgattt ttattcaaag tcagattcca gagctagctg aattgagcat gtattatatg
     3901 aatctggtat cattagcacg tttggaaaga aatcccactg ttaaaaccga aattgctcag
     3961 aaaggttttg gcgaaagtat tccatctggt tttttggttt atccagtatc acaggccgct
     4021 gatattacag catttaaagc gaatttagta cctgtaggta acgaccagaa accgatgatt
     4081 gaacaaacac gtgaaattgt gagaagtttt aatcatactt accatacaga ctgtttagta
     4141 gaacctgaag gtatttatcc agaaaatgaa aaagctggac gcttacctgg tcttgatggc
     4201 aatgccaaaa tgtctaaatc attgggaaat ggaatctatc tctcagatga tgcagatacc
     4261 gttcgcaaaa aagtgatgag catgtatact gatccaaatc atattaaaat agaagatcct
     4321 ggtcaaattg aagggaatat ggtctttcat tatttggata tttttgctag aaaagaagat
     4381 caagctgata tcgaagcaat gaaagagcat tatcaaagag gtggtttagg agatgtgaaa
     4441 acgaaacgct accttctaga tattttagaa cgtgaattag cacctattcg tgaaagacgt
     4501 ttggagtacg ctaaagatat gggagaggtg ttccgtatgt tacaagaagg tagtcaaaaa
     4561 gcaagaactg tggcagccaa gactttatca gaagtga
//
LOCUS       DAUCWU010000103         4452 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUCWU010000103 DAUCWU010000000
VERSION     DAUCWU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.103"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(30..1298)
                     /locus_tag="VGY24_001584"
     CDS             complement(30..1298)
                     /locus_tag="VGY24_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ4284546.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            1388..2254
                     /locus_tag="VGY24_001585"
     CDS             1388..2254
                     /locus_tag="VGY24_001585"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ4284547.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2232..2789
                     /locus_tag="VGY24_001586"
     CDS             2232..2789
                     /locus_tag="VGY24_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ4284548.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRVPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2923..>4452
                     /locus_tag="VGY24_001587"
     CDS             2923..>4452
                     /locus_tag="VGY24_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ4284549.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DF"
BASE COUNT         1430 a          665 c          872 g         1485 t
ORIGIN      
        1 attttttata ttaatggtac gaagaaaagt tagttgctct ttccacaagt atttttgatt
       61 agtgagttta atttgctttg tatttgttga ttgatagcaa tagagagata gcgttttttt
      121 ccagttatta ttattctatc agatttatct aatgtaaagt ttctataaga gtctgatgtc
      181 gtgtaatcaa tcactaaatg agtaggggtt agttgatagt gaatatcagg gcaattttgt
      241 tttaaaaaga gagccaattg acgttcccgc tgttttagat aacgcttaat aaagcgaaga
      301 tttttttgaa acatcccatt ttctaaataa agagctaatg ctttttgcat tagtaagttc
      361 gtgtctaaat caatcagtga tttttgggtt aaaaaatgag gttttaaacc tgacggtaaa
      421 actagagctc cgatacggag tgcaggaaaa acactcattg aaaaagattt taggtagatg
      481 attcggtgat gagtgtcata ataatgaatt ggtggctctt taagtttcac aaaatctcct
      541 aaataatcat cttctaaaat ataaacctgg tatcgttgtg ctagacgaac aatagcttcc
      601 ttttccttag tgctataaga aagtcccaga ggatgtgaaa atcgtgaaat cgtgtaaaaa
      661 aatttaatat caccagtttg aaataaagat tctaagagtt ctaaatcaag gccattaaag
      721 tgtctagaga tagtttgata gggtaatcct aactgagcaa caatagcttc catccgatga
      781 taagtaggct tttcaaggag aattgttttg cccgcattag gaaaaggcat ttgtgataaa
      841 atataaagcg cttgctgcgt cccagaggtg attaatagtt ggtctttgtt actgtaaaca
      901 ctattttcag ctaagtaaag aagcaaagct tcccttagtt cttctagtcc ttcggttttg
      961 tgataataat gaaagaggta tttgtcttta gcacttaaag cctcatttaa gcagagtcgg
     1021 aaatcttcat aagccatatt attataatct tcaagactta gattaagaac attatcgggc
     1081 atgcttacgt tacccaaaac ataataacca cttttaggaa cagcataaat taagtgacga
     1141 tattttaatt ctaaaagagc ccgctgaaca gtatctttgc tacaatgata aaccttactt
     1201 aataccctaa ttgaaggtag cttgtcccct ttttttagtc gctgtttctg gatatcttgt
     1261 tcgatattgg aaatgattgt ttgatatttt gttgtcataa attgtcccca tacagttaag
     1321 tgttataact attgtatctt atgcctcttt ttcttacaat agttattata gaaaagaggt
     1381 aatagctatg aaacggatta ttgtggcaaa tgatctggtt ggtgttggta aggtagcttt
     1441 gtctgctagt attcctttaa tggcttcttg tttgttagag caagttttat taccaacttg
     1501 ccttttatca agccatactg gcggaggaca gagccctgtg aagcatacta catcaagtat
     1561 attagacgtt tttttaacag attgggacaa caaaagaatt aagtttgacg gcatttttat
     1621 aggttatttt acgacaacaa gagatgggca aaagctgcta gaatggcttt caagaaagca
     1681 acttccctta attttagatc caattatggc agataatgga aaactttatc aaggcttcag
     1741 tcaaaaacat atagagatta tgataaaact tactcaaaaa gctgagcttg ttttacctaa
     1801 tattagtgag gcttgtctat taacacaaac agcttattta gcaaaaaatt atgatgaaga
     1861 agatattaga caggtggcta gaaaattagc taagcttgga cctaaacagg ttgtcattag
     1921 cggaatctct tttgaggagg gcagcattgg ttttgcttac tatcagtcag attcagatca
     1981 attaacattc cattttggga agcaattccc ttaccatttc tatggtacag gagatatagc
     2041 atcagctatt ataacagcag gattctatta tcagctacca ctaacacaaa ttctagatct
     2101 tgttgtttca tttcttgaaa aagtattgca aaataccctt acattaaaaa gagatttaat
     2161 cttaggtgtt gcttatgaat ctcatttaaa ttacttaact aaatcgttta aaaagttgtt
     2221 ggaggaaaaa aatgtcatct caaaaattag ctaattatat tgaattgagt atgtatgccg
     2281 ctttaatttt tatttcagta caagtgttgc gtgtccctgt cggtatgcag tttattcatc
     2341 ttggcaatgc attagttgtt attgcagtct taatctatgg tagtaaaaaa ggagctctag
     2401 ttgctagtct tggtttaggg atctttgatg ttttgaacgg ctatgcttca gtcgtttgga
     2461 taactatctt agaatccttg gctatttgtt taattttgca tttggtatat gaaagagcta
     2521 tgcaatctag taaaaaacca aaagccatta taacaataac agttattgct gcattgtcaa
     2581 aattaactat taaccttata aaatacttac tgttagctta tataggtact cagctaccac
     2641 tttcggtatc tttttgggta gctcttggaa aaattggtgg tagtttcgga acatcttttg
     2701 tgactttagt tgctgttcct cttttgtatc ctgttatcaa aaacttaaaa gaaaaggtta
     2761 caatcggaca agcaaaaaga ggtttttgat acaagtacct tatcgataaa aaagcaaggt
     2821 acacttttag tcaaatattg acaaaaggtt acaattttgc cataatgaaa taactataaa
     2881 gccgttttga cggctttttt gatgacagtt aaggaagaaa cgatgagtat tttagaagtt
     2941 aagcagttga gtcacggttt tggggatcgg gctatttttg aaaatgtgtc atttcgcctc
     3001 ttaaaaggtg aacatattgg actagttggg gcaaatggtg aaggaaaatc aacctttatg
     3061 agtatagtca caggacattt acagcctgac gaaggaaaag tagagtggtc gaagtatgtc
     3121 actgcaggtt acctggatca acatacagtg ttggaatcag gacaaaccgt tcgtgatgtc
     3181 ttgcgaactg cttttgatga gttatttaag accgagaatc gtattaatga gatttacgcg
     3241 tcaatggcag atgataaagc tgatattgct gttttgatgg aagaagtagg tgagcttcaa
     3301 gatcgtttag aaagtcgtga tttctatact ttggatgcta agattgatga agtagcgcgt
     3361 gcgcttggtg ttatggattt tggaatggag tcagatgtta catccttatc aggtgggcaa
     3421 cgaacaaagg ttttattagc caaattacta ttagaaaaac ctgatattct gctattagat
     3481 gaaccaacta accatttgga tgctgagcat attgaatggt taaaacgcta tttacaacat
     3541 tatgaaaatg cttttgtgtt gatttcgcat gatatttctt tcttaaatga tgtgattaat
     3601 attgtttatc atgttgaaaa tcaaagttta gttcgctata ctggggatta ctaccaattt
     3661 caagctgttt atgagatgaa acaatctcaa cttgaagcag cctatgaacg tcaacaaaaa
     3721 gagattgcta acttgcagga ttttgtcaac cgaaataagg ctcgggtagc gacacgtaac
     3781 atggcaatgt ctcgccaaaa aaaacttgat aagatggata taattgaact tcaagctgag
     3841 aaaccaaaac caaattttga atttaagcaa gctagaactc ccagtcgatt catttttcaa
     3901 acaaaaaatc ttgtgattgg ttatgattac ccattgacca aagaaccctt aaatataacg
     3961 tttgaaagaa atcaaaaaat tgctattgtt ggggccaacg gtattggaaa atctactttg
     4021 ctaaaaagtt tattaggtgt tattgagcct ttagaaggtc atattgtcac aggggatttt
     4081 ttagaagttg gctactttga acaagaagtg acaggtgtta accgacaaac tccgctagaa
     4141 gtagtttggg atgcttttcc tgccttaaat caggcagaag ttcgagcggc actagctcgt
     4201 tgcggactaa catcaaaaca tatcgaaagt caaattcaag tactttcggg tggtgaacaa
     4261 gcaaaagttc gtttttgttt gttgatgaat cgtgaaaata acgtgcttat tttagacgaa
     4321 ccaacaaatc atcttgatat tgatgctaaa aatgagctca aacgtgcttt aaaagcatat
     4381 aagggttcta ttttaatggt ttgtcatgaa cctgatttct acaatgggtg ggtaaccgat
     4441 acttgggatt tt
//
LOCUS       DAUCWU010000104         4446 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUCWU010000104 DAUCWU010000000
VERSION     DAUCWU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.104"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            347..961
                     /locus_tag="VGY24_001588"
     CDS             347..961
                     /locus_tag="VGY24_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEQ4284550.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKDKSKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(1375..2247)
                     /gene="sdaAA"
                     /locus_tag="VGY24_001589"
     CDS             complement(1375..2247)
                     /gene="sdaAA"
                     /locus_tag="VGY24_001589"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ4284551.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(2259..2930)
                     /gene="sdaAB"
                     /locus_tag="VGY24_001590"
     CDS             complement(2259..2930)
                     /gene="sdaAB"
                     /locus_tag="VGY24_001590"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ4284552.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            3228..4349
                     /gene="mnmA"
                     /locus_tag="VGY24_001591"
     CDS             3228..4349
                     /gene="mnmA"
                     /locus_tag="VGY24_001591"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ4284553.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT         1425 a          778 c          848 g         1395 t
ORIGIN      
        1 tattagtaag aagtgaattg tacctcaaaa gttagataca acatctaatg attgaggtac
       61 ttttcttcat ttgaggaatt gaaaaccgct atctcaacta tattaatcct cccaataata
      121 acaaatcgaa cgaaatctaa aaggactgag tcctgttcaa tacagaacta aattattagt
      181 ttaatgatat tatcccgctt tttaatgttg ctaagaagaa gagttttatg attttttaca
      241 acttcttata atatcaatgt agttgtaata tttttaatat atcttcaata acgaggtaat
      301 aattaaaaga tattatggat aacggagaaa agtgaggtat tattgaatgt ttaagaaaga
      361 aaatttaaaa caacgttatt ttaattttgg attagtagcg ttagctctaa caatattagc
      421 catcattttt gccttctcaa gtaaaaatgc tgatactaag tcttatgcta agaagtcaga
      481 aagtaaaatg gtaacaatcg acaaggctcc aaaaaataat catgctatta ctaaagaaga
      541 aagcaaagat aaatcaaaga gcattgcttc ggagcctatt cccacagtag aaaactctgt
      601 agctccgaca gtaacagagg aagcaccggt tgttcagcaa gaagtgactc aaactgttca
      661 gcaggtatct tcagtagcct ataatccaaa caatgtggta ctttccaatg gaaatactgc
      721 tggtattgta ggaagtcaag cggcggcaca gatggcagca gcaacaggtg ttccacaatc
      781 aacttgggaa catataattg cgcgtgaatc taatggaaat cctaacgcag ctaatgcttc
      841 tggggcatca gggttgttcc agacaatgcc aggttggggt tctacagcaa cggttgaaga
      901 tcaagtcaat gcagccttga aagcctatag tgcacaaggt ttatcagctt ggggttacta
      961 aataaacata gctggaagaa ataaaaccta ttgttagtat ttactaacaa taggtttttg
     1021 gtatttaatt gtctttatga tttgtagtga tgaattagtc tcaaaacaaa acattgcctt
     1081 atcagggaat atagctcgta ttatttagga aaactgacca gcacttaagt gcctctgata
     1141 tttaaggcag ttatactgat aacaagcagg aaaaaaactc ttaaatgttt ataagatcat
     1201 tagcaatttt cagtatttta cactactaca cttttgagtt ttaacaattt actagaagtt
     1261 gtttctaagg ggaaacaaca tagcttgata gctatctatt agtcatttta gtgaatatat
     1321 agcagaaaaa taaaacgctt tcaaatattt ctaagaattt ttctaactat ctatttattc
     1381 gccaaatatt tctacgctgt aacgtcgtcc agttggagta gcggctaaac caccttctgc
     1441 tgtttcacgg aaagcagttg gcatggcaga gccgacttga tacatagcat caataacttc
     1501 gtcaacagga atttgagaat caatatcagc taaagccata tcagctgcga caagggcgaa
     1561 acttgctcct agggcattgc gtttgacaca aggcacttct actaggccgg caacaggatc
     1621 acagacaagt cctaataaat ttttgataac aaacgcaata gcttgactag cttgatgaga
     1681 tgttccacca gctgctttaa caagagcagc tgcactcata gcagctgcag aaccaacttc
     1741 tgcttggcat ccaccttctg caccagaaat agaggcgtta tttccaataa caagaccaaa
     1801 agctcctgca gtaaataaaa actctaattg ttctttttct gataagtcaa gtttttcaat
     1861 agctgttgcg agcactgctg gtaagcatcc tgcacttcct gcagtgggtg ttgcacagac
     1921 aagtcccatt ttagcattta attcgtttac agctatagca tttctaacag cattaagaat
     1981 agtagtatca gaaaggctgt tccctttttt aatgtagtta tccattttga ccgcatcgcc
     2041 ccctgtcaaa ccactgatag atttggttga ggttagtcct tctgttacag ctgctttcat
     2101 aacttgtaaa ttgcgtgaca tgattttgat aatatcttct cgatttcttc cagacatctc
     2161 cacttccgtg gcaatcatga gttcagcaat atttccatta aattgttggt cagcttgttt
     2221 gacaagttct tcaatagtat aaaacatagc tttcctttct agtcaaagaa gttgacatta
     2281 taaatatgag gaatttttgc aatttggttg gctgcttctt ggcactctct agaatcgact
     2341 tctataatca tagtagcttt ttcaccagcg gattcacgtg taacattcat tgtggcgata
     2401 ttaatattat tggaagatag gatatcagtt acctttgcga tcattccggg aatatctttg
     2461 tgtacagtaa ctatagtagg ggtattcatg gataacgaga cagaaaaacc atttagttct
     2521 gttacttgaa tgtttcctcc tccaattgaa acaccagtaa cactaagtgt tttgtcagct
     2581 tttttgacac tgattttaac agtattggga tggggagcat tactatcttt taagatatcc
     2641 caatagattt ttatcccttt ttgatgagca atttctagag aatttttaat atcaggatta
     2701 tcagtatcca tacccataat tcctgcaaca agagccttat cagtaccatg tcctctgtag
     2761 gttttagcaa atgaattata aaggtgaaac gtcacttcat caggaatatc tccaaagatg
     2821 gaatggacta ccttaccaat tctgacggca ccagctgtgt gactacttga tggaccaatc
     2881 attactggcc caatgatgtc aaaaactgat tgaaattttt gtgtattcat aggcaactcc
     2941 gttttttatt tctatgttta ttataacaaa aaaatatcaa aaaacagcaa tataattata
     3001 aaaaattatt tttataataa aaaattattg agcaataaaa tgattggcat tccacgcagt
     3061 aattgttata ataaggtttg cgctgatggc gcttcaaaat cgaccttcaa atcgactttt
     3121 gaaaacgacg accttcaatc gtacgttaac tgataagaaa aagaatcata atattcataa
     3181 tatgatgatt atctttttct atcttattat tgatgggaga attttttatg acagataact
     3241 caaagatacg tgttgtcgtt ggtatgagtg gtggcgttga ttcatctgta acagctctac
     3301 ttttaaagga gcaaggttat gatgttattg gtgtcttcat gaaaaactgg gacgatacag
     3361 atgaatttgg tgtttgcaca gctactgaag attacaaaga tgtagcagcc gttgctgatc
     3421 aaatcggtat tccttactat tcagtcaatt ttgaaaaaga atattgggac agagtatttg
     3481 aatatttctt ggccgaatac cgagcaggtc gtactccaaa tcccgatgtc atgtgtaata
     3541 aggaaataaa atttaaagct ttccttgact atgccatgac tttaggtgca gattatgtag
     3601 caacgggaca ctatgctcaa gtcaagcgtg atgaaaatgg tacggttcac atgttacgtg
     3661 gagctgataa tggcaaagac caaacctact ttttaagtca actatcacag gaacaactac
     3721 aaaagacttt attccccctt ggtcatttgc aaaagtcaga agttagagaa atcgctgagc
     3781 gagcagggct tgcaacggct aaaaagaaag attcaacagg tatttgtttt attggtgaaa
     3841 agaatttcaa acagtttttg agccagtact taccggcaca aaaaggtcgc atgatgacta
     3901 ttgatggtcg cgatatggga gaacatgctg gtcttatgta ttatactatt ggtcagcgtg
     3961 gtggtctggg cattggtggt cagcatggtg gggataatca accttggttt gttgtcggta
     4021 aagatttgtc gcaaaatatt ttatacgttg gtcaaggttt ctaccatgaa gccctaatgt
     4081 caaatagtct tgacgcctca gtcatccatt ttacacgtga gatgccagaa gagttcacat
     4141 ttgaatgtac tgctaaattt cgctatcgtc aacccgattc acacgtggcg gtgcatgttc
     4201 gaggagataa ggcagaagtt gtttttgcag agccacagcg cgcgattact cctggtcaag
     4261 ctgttgtttt ctatgacgga aaagaatgcc ttggtggtgg tatgattgac atggcttata
     4321 aaaatgggca gccttgtcag tacatttaat gaatctttta ttaagataaa aagaaaggga
     4381 cattcaacaa ttcctttgac atcatatgat attatttata aaaaccaatc atgcgttaat
     4441 ggatta
//
LOCUS       DAUCWU010000105         4327 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUCWU010000105 DAUCWU010000000
VERSION     DAUCWU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.105"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..707)
                     /locus_tag="VGY24_001592"
     CDS             complement(<1..707)
                     /locus_tag="VGY24_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ4284554.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQICLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            complement(773..1969)
                     /locus_tag="VGY24_001593"
     CDS             complement(773..1969)
                     /locus_tag="VGY24_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ4284555.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            complement(2346..4151)
                     /gene="pepF"
                     /locus_tag="VGY24_001594"
     CDS             complement(2346..4151)
                     /gene="pepF"
                     /locus_tag="VGY24_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ4284556.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFA
                     GHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPIVRNDKGENVELTHGNFISLMESKDRSVRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            complement(4164..>4327)
                     /locus_tag="VGY24_001595"
     CDS             complement(4164..>4327)
                     /locus_tag="VGY24_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ4284557.1"
                     /translation="WRPVQSSSGFCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKY
                     YVKMDSNRK"
BASE COUNT         1411 a          864 c          720 g         1332 t
ORIGIN      
        1 taatctggta atacaatatc tgcttgattt ttacgaatca ttaataatcc atcactaaga
       61 gggaccaggc tagttgttag gtttggatga gttaaggtgg catcaaacaa agactgtaag
      121 ccacgataaa tagtacgctg acctcgtcga atgtcttcga tcggttttgt aatgtcacct
      181 ccttgaaaga catcatccaa aatgactaca ccaccgactt ttagcagtct taaaatctct
      241 ggtagaaaga caatatattt ggatttagcg gaatccataa acacaaagtc aaaatttcct
      301 tcaagtgtag ataatatatc tgctgcatct ccttctaaca ggcagatctg ttggcgactg
      361 tcgtacttag caaaatttgc tttagcaaaa tcgatcatct ctcgattacg atcaatagtc
      421 acaatcgtcg catctggtgc attttcagcc attaaaagcg cagaaaaacc aatggctgtg
      481 ccaatctcca aaatgtgttt tggctgtaag ctttgcaata aaaacctgaa ataagccacg
      541 acctcaggct gaataatagg aatgttttct tgtcttgcga attgctctaa ctctgctaaa
      601 aaacctgtgg tttgcttctg ccgtgttcgc atataatgaa caagctcttc tttgacaaca
      661 ggacgacgca tattatgatt tgctgtttta ctgtatgatt taaccataaa cctaattata
      721 taaaaaaacc ctaggctttg ctagggttta cttttaaatt ttttatagga catcatttct
      781 ttaaccttaa tgctaccatt aatcctaaaa tgtataagac aataaaaaca cataaggtaa
      841 gaagataatt atgagtccat tcaacagtga tagataataa cataggtcca gttaatgcag
      901 ctattgccca agctgtcaat atatacccat gtaatgtagc cagttccttt gccccaaaaa
      961 gatcacttaa ataaggcgga atgagagaaa aacccgcacc ataacaagtc ataagggtgg
     1021 caatgcttat catgaaaatt agggaactat gtgcaaatat taaactaata gtcataatta
     1081 tgcttaccaa aaataaaagt attactgtca cacggcgtcc aatatagtct gataaactag
     1141 cccacactaa acgaccgaaa ccgttaaaaa tccccatagc accaaccaca attgctgaca
     1201 tttctggact cataccagtt agatcttgtg ccattggcgc aacaactgag ataagtccta
     1261 agccacatgt gatattgata aataaaatta cccaaagaca ataaaatgat tttgttttaa
     1321 gtgcttcttt agcggtcatt ccttcgatta aataactgtt attttgtaat ctttttttat
     1381 ctagtatagc tatctcagca gcagttggtt ttattatcaa ttgagaagca aaaagcataa
     1441 ctattagata aatcagccct aaaaggtaaa atgttgctac caaaccttct gtttcaatta
     1501 gccactgggc aatcggactt gttaataaag acgcaaaacc aaaccccata atagcaaacc
     1561 ctgttgccat gcctctttta tctggaaacc attttataat agttgatatc ggtgtgatat
     1621 atcccgctcc aagtcctaag ccaccaataa caccataacc aatatataaa agccaaattt
     1681 ctttacgatc aatcgctaaa ccagtcagca tatttccaga agcataaaga atagctgaca
     1741 ccgttccggt tagacgagga ccatattgtt caactaaatt tcccataaaa gcagcagata
     1801 aacctaaaca aaaaatagct aaagaaaagg caaaagcgac aggagcttgg tcccaaccgg
     1861 tctcttgtaa aattggatta cgataaacac tccaagcata ggtcgatccc aacattaagt
     1921 ggagaagtat ccctgctgtt gcaatgatat aacgttttgt tttttccatg aaaaactcct
     1981 tttttattat acgaacgttt attttaaaaa aaacataaat gttagtattt taattctatt
     2041 atctctttta taaaatgtca aatataataa aaatatgttt tatgcgaacg ttatctctaa
     2101 aacaatagaa tagcgttaat ctgaagcgcc ttactcaact attggtttta tttaagtgag
     2161 tgaccacaca ataaaaaaag acaaaaaaga aaccgtcaaa ctaaggtcaa tgaagcctgc
     2221 ttttcaggac tgataatcca ctaaaatata aaaagaaaat gaccgataga aatggagatt
     2281 cacaccaact gtgaatctcc atttctatca atcctaaact ctttttgtcc ttgacttttg
     2341 tgattttagc ttttgtgtat gcctttggac acgagtactt caagttctgt tagacgttcg
     2401 tcaaagactt taaaggcggc ttccaaataa tcaccttttg ccatatcaac gcctgcttta
     2461 gcaatcactt caagtgggta atcagaattt cctgacttga ggtaggccaa gtaatgatcg
     2521 atatcatctt gtgtgccatg cacaatctta tccgctaaat agcttgcagc tgcaaaacct
     2581 gttgcatatt ggtaaacata ataattgtaa tagaaatgag gaatacgtgc ccattcatac
     2641 tgaataaaat gattatcttt tttacttaaa ccataatatt tttcatttaa atcagcgtat
     2701 agctggttaa ggtattcact tgttaacact tctccttttt gatcagcttg atgaatggca
     2761 tgttcaaact cagcaaattg ggtttgacgg aaaactgtcc cacggaaacc atcgaggtaa
     2821 tggtttaaaa tggcaaagcg ttctttctca tcttgcactt catttaagag agcttctgtc
     2881 ataatatttt cattcgttgt ggatgcaatt tctgccaaga agatactata atccccatac
     2941 acataaggct gtgtttcacg agtgaaagta gaatgcaaac tgtggcctgt ttcgtggaca
     3001 agtgtgtaaa gattatcaag tgtatcttgc cagttcaaca gcatgaaggc attggtatca
     3061 taggaaccac ctgaataagc cccagagcgc ttccctttat tgacgtgaac atcaatccaa
     3121 cgttcggtaa aagcacgatg tacacgatct gcgtaatcct taccaaaaac agccaaaacc
     3181 ttctcagcct tttccaaagc ctcatcataa ccaatggcta aatctgtttc agataatggt
     3241 gtgtaaacat catacatctt caaatcgtct aaaccaagaa cctcttgacg caatttcaaa
     3301 taacggtgta agagtggtaa atgtttgtta accgtttcta ataaagtgtc gtaaacagct
     3361 tctggaataa agttagcagc catggcagct tgacgtgctg aatcgtattt atgaacacgc
     3421 gccttatagt tttgcacttt cacattggtt tgcaaggttt tagcgtaagt atgttggaac
     3481 tgttcataag tactatacat ggcttcataa gcagcttggc gaacgctacg gtccttagat
     3541 tccattaggc taatgaagtt accatgggtt aattcaacgt tttcaccttt atcatttctg
     3601 actatcggga aaacaatgtc agcgttatca agaatactaa aggtttcttc cgcaccattg
     3661 aaaatttctt gagctcctgc taataattcc tcttctgctt gactaagaac atgttcgcgg
     3721 gcttggaata atttgtcaaa gaaatggttg tacactttca actcaggggt ttcagtaagg
     3781 aaagcctgat agtcttcttg atgaagcatc atcacttctg ggtcatagaa tgaaaagact
     3841 tcgctaaact ttgcatacaa gcctgaagcc ttagcttggt attcttgata cttggcaaca
     3901 gtagtgtctt ggtcattttt catgtgagca taaacataga ctttctccac acgacgagcc
     3961 aattctaaat atgtttttgt aacctttaac aaattagcac tagaatccag caaatgacca
     4021 gcaaaacctt tagatgcctc aacttcagta gctaagtcac tgacttcagc ttcccaatcc
     4081 ttatctgttg caaaaatagt gctcaaatcc caagtgtatt tttcttctaa atggctgcga
     4141 ttatcagtca taacatctcc ttctcatttt ctattactat ccattttaac ataatatttt
     4201 ggtgaaaaaa gcgtttgcac cttcttatcc ttttcttttt tgtaatagac tttaaaactt
     4261 tcataataag ggcgaagatt tcctttaatt tgacaaaaac cgctggaaga ttgaactggt
     4321 ctccatt
//
LOCUS       DAUCWU010000106         4288 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUCWU010000106 DAUCWU010000000
VERSION     DAUCWU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.106"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     regulatory      148..234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            362..1684
                     /locus_tag="VGY24_001596"
     CDS             362..1684
                     /locus_tag="VGY24_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ4284558.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKIPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITYLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     gene            1804..3039
                     /locus_tag="VGY24_001597"
     CDS             1804..3039
                     /locus_tag="VGY24_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ4284559.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(3409..4182)
                     /gene="cfa"
                     /locus_tag="VGY24_001598"
     CDS             complement(3409..4182)
                     /gene="cfa"
                     /locus_tag="VGY24_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ4284560.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIETTVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
BASE COUNT         1208 a          807 c          797 g         1476 t
ORIGIN      
        1 tttttgctat tttggttgac ttcattcgtt ttttgaggta tgatagtcct aatttaataa
       61 accgaatgat gtcatgcagg agaagaattt ttttcgccga aggagttata ctctcaggtg
      121 ttcagttttt gaacgggact gtttgatgga cggacttctg gagagacctt attaggcgcc
      181 gaaggggcaa ggcatactgc tcaatctctc aggcaaaagg acagaaggta aaatacaaac
      241 accattaaga acagtcttag tcttttttgt gtttgctgtt ttatcatcgc ttcagaagtt
      301 gtctcaaaga aaaagatagc ttttttcttt tggcttcttc gatgactttt aggagagaaa
      361 gatgatagca ctcgttaaat taattgataa ccttgtttgg ggaccgcccc tcttaatttt
      421 attggttggg acggggattt accttaccag tcgtttagga ttaattcaaa tcttaaaaat
      481 accaagagcc tttaaactca ttttttcaga tgacgaagga catggagata tttcatcctt
      541 tgctgctctt gcaactgccc ttgccgctac tgtcggaact ggtaacattg ttggggttgc
      601 cactgctatc aagtctggtg gtcctggagc gctcttttgg atgtgggttg ccgctttttt
      661 tggaatggcc actaagtacg ctgaaggggt actagcgatt aaataccgta caaaggatgc
      721 caacggtcat atttctggtg gtcctatgta ctatattgtt aatggcatgg ggacaaaatg
      781 gaaaccctta gccattcttt ttgcgggttc agggatccta gttgccctat ttggaatcgg
      841 aacctttgct caagtgaact cgatcacctc gtctttaggt catagctttg gcttatcccc
      901 acaaatggtc agtattgttt tagctatctt tgtggctgcc attatttttg gtggcattca
      961 ttccatttcc aaagtggctg aaaaagtcgt tccttttatg gctatttttt atattctatc
     1021 tagtttagca gttattttta gtcactacca gcagctactt cctgttattc gtttagtctt
     1081 tcaatcagcc ttcactccga cagctgctat tggaggcttt gctggaagtc tcatgaagga
     1141 tgccattcaa aaaggaattg cacgcggtgt cttctccaat gagtctggac taggatctgc
     1201 acctattgct gcagcagcag ctaaaaccaa tgaacccgtg gagcagggac tgatctccat
     1261 gacaggaact tttattgata cgattattat ctgtacccta accggcctat ctattttagt
     1321 aacaggccaa tggactgggc agctcgaagg ggctcctctg acccaatctg cctttgccac
     1381 tgtgtttgga aatttaggga cttttggcct aaccttttcc ctagttctct tcgcctttac
     1441 aacgatttta ggatggagtt attatggaga acgctgcttt gaattcctct ttggcattac
     1501 atatctcacc tacttccgta ttgtctttat cttaatggta ggcctaggag gcttccttaa
     1561 gttagaactg atttgggttt tggctgatat tgtcaatggt ttaatggcac ttcctaacct
     1621 gattgccctc ctagctctct cgccagttgt tattttagaa accaagcatt actttattaa
     1681 gtaaacatta cgttagtact acccagttat acctaggctt cttctgccaa gttcaagaag
     1741 ctaggatttt ttcttaagcc cctttttatg gtataatata aagttgaaat caaaggagtt
     1801 attatgacac aggatccgat tgcaaattta aaactagcca gaaagggccc aatcgttagt
     1861 attattgtct acttgttgct tagtgttgcc aaattattag ctggttattt gttaaatgca
     1921 agttccctta ttgctgatgg atttaacaat ttatcggata ttgtgggaaa tgtagccctg
     1981 cttattggtc ttcacttagc tagccaacca gccgatgcca atcataaatt tggtcattgg
     2041 aaaattgaag acttatccag ccttgtcact tcttttatta tgtttcttgt aggtttccaa
     2101 gtactgattc acacaattaa aagtatcttt agtggtcagc aagttgatat tgaccctctt
     2161 ggggctattg tcggtatcgt ttcagctttt gttatgttag gggtttatgt ctttaacaaa
     2221 cgtctttcca aacgtgtaaa atccagtgcc ttagtcgctg cttctaagga taatctagct
     2281 gatgctgtta cttctatcgg aacatcaatt gctattatag cagcttcttt gcatttacca
     2341 gttatcgatc atatagctgc tatgatcatt acgttcttta ttcttaaaac agcttttgat
     2401 atctttatgg aaagttcgtt tagtttatct gatggatttg atagccgtca tttgaaaaaa
     2461 tacgaaaaag ccattttaga aatccctaaa attgtcgccg ttaagtctca acgagctagg
     2521 acctatggta gcaatgtcta tcttgatatt gtacttgaaa tgaatcctga tctttcagtc
     2581 tatgaaagtc actctattac agagaaagtg gagcagttat tgagtgacca attttctatt
     2641 tatgacattg acattcacgt tgagcctgcc atgattcccg aagaagagat ttttgataat
     2701 gtcgccaaaa agctctaccg ctacgaaaaa ttaattttga gtaaggttcc tgactatgac
     2761 cactacattg ctaagtcttt ccaactgatt gatgcgaatg gccaaacagt taactatgaa
     2821 caatttttga accaagaaat ttattatcca agtaacttca accattttca gattgaatcc
     2881 attagtcaaa aaacgatgtt ggtaacttac caattaaatg gcaatcaaca taccagtatt
     2941 tggaggcgtc atgaatcttg gtccttactc ttccaccaaa ttacccctat cgctaagaga
     3001 caattacatc acacacacta tcgtattgta aaaatgtaaa aacattactt ctcttataac
     3061 ataactatac ttcatgatat tcctaaagcg attcctagtc ccaaaatgat aagtgaaata
     3121 gttaaaaaaa tccctacaat ttgtttttaa acagatcata gggatttttt taagaataca
     3181 cgttttcata ttgaatctta ttagtttagg aggttaaaca agattcaata gtgacggttg
     3241 aacaatacct taaattggta catcatgtta aaggcaaaaa gggctacagg tgactcattg
     3301 tttatgagag catcacctca aactctacaa tggtaagata tttaaaatct ttacctattc
     3361 catatcctaa tataatcatc tacctataca acacattact tatacactct atgacttaag
     3421 agctgtttcc aaagcatttt gcacagcaat aacttcatcg tcaacttgtt gaacagtggt
     3481 tgtcggattt agttggacac caaccgcatg tgtgatagct ttgttaagtc tattgtaaac
     3541 gtcaaatggt ttgatcccca aaaccttttt atctctttct aaacgagtgt tccaaatagc
     3601 tttatttagt ttagctttag tatagatagt agcacggtct gttggctgta aatcaggata
     3661 attgataact ttttcctcaa gtgctttaat ttcttgtact tgagctttga tagcgtcaac
     3721 agatgcaaat ggatctataa tacgaatgac taatttagta atagcaaacc ccatatcagt
     3781 atgcgctttt tctactttat gagttaattg ggttgtggaa aatacgattg ctttgatagc
     3841 atctgataat gcttctactc ttgccccaat tgaactaaaa tcatagatcg tttcagtatt
     3901 gaagcgtata gaactcttca tgtgatcaac agcatcgata gctttgttaa cagtagtctc
     3961 aatatcagta cctttagctt catgtttaat agcttctaat tgttgatgtc cctcatcaag
     4021 cttttgcact agagcatctc tttgaagatt cgcttggttg ttattcatta aaatagtatc
     4081 gggatttgaa gcatcatcag ctaaaactga gtgcgtcata gttgaaaata aagctaatcc
     4141 agcaacaact gttgaaagac agagaaggtg tttgggtttc atattattcc tcctgaatat
     4201 aataaaccta tttaaatagt taagtgaagt taaattaatt tataagcgct tataacgtta
     4261 tcatactact tgtttttact aaggtcaa
//
LOCUS       DAUCWU010000107         4285 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUCWU010000107 DAUCWU010000000
VERSION     DAUCWU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.107"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            141..989
                     /locus_tag="VGY24_001599"
     CDS             141..989
                     /locus_tag="VGY24_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ4284561.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(1324..2220)
                     /locus_tag="VGY24_001600"
     CDS             complement(1324..2220)
                     /locus_tag="VGY24_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ4284562.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            2518..3270
                     /locus_tag="VGY24_001601"
     CDS             2518..3270
                     /locus_tag="VGY24_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ4284563.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKINQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            3255..4205
                     /locus_tag="VGY24_001602"
     CDS             3255..4205
                     /locus_tag="VGY24_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEQ4284564.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKGNKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
BASE COUNT         1374 a          740 c          819 g         1352 t
ORIGIN      
        1 ttttctttac cgcttttatt attgtgtgaa aataacactt aatgatcatt ttatttatta
       61 ttttattacc tttacattca tcaataagcc tgttaaaatg gtatttgtta tattttatta
      121 tttggaggaa accagaaaac atggaaatga catggactgt gaaatacatc acagaattta
      181 tcgcaacggc ttttttgatt attttgggaa atggagctgt tgctaacgtc gacttaaaag
      241 gaacaaaagg acataactca ggttggttag tgattgcttt tggctatggc ttaggagtta
      301 tgatgccagc tttgatgttt ggtaatgtat ctggaaacca tattaatcct gctttcacag
      361 ttggactagc agtatcaggc ttgttcccat gggctcacgt cttacaatac gttgtggcac
      421 aattacttgg agctatcttt ggtcagttgg tagtggtaat ggtttataaa ccttacttta
      481 tgaaaactga aaatccaaat catgttttag gctcattctc aacgatttca tctcttgata
      541 atggtcaaaa agacagtcat aaagcctctt atatcaatgg tttcttaaac gaatttgtag
      601 gttcatttgt tcttttcttt ggtgctttgg ctttgactaa aaactacttt ggggtagagt
      661 tagttggtaa attgattgaa gcaggctacg accaaacaac tgcagcaaca caaatttcac
      721 catacgtgac aggctcattg gcggttgctc acattggtat tggtttctta gttatggtct
      781 tggttacttc attaggtgga ccaactgggc cagcactaaa cccagcgcgt gattttgggc
      841 ctcgtttgtt acaccacttc ttaccaaaat cagttcttgg tcaagccaaa ggtgattcaa
      901 aatggtggta tgcatgggtt cctgtagttg cacctatctt agcggcgata gtagccgtag
      961 cagcgtttaa atacctttat atcagataaa aaaagaacta aagcttcttt tagactcaag
     1021 acaagctcgc ttaaggaact tgtctttttt atatgagtta agcgaaatct aatgcaaact
     1081 tgactgtctt gtatagcgta ttttgtaaat tttattagac ttcttgtaaa aagcctctcc
     1141 tcagataaga tgactaatgc ctctaaacgg tgttacaaat aaacgaggca tccctaactt
     1201 cctttatctg gtgttgaaac tattttgcac ggactaaatc actgacagag taaaaaacga
     1261 tttttcattt ttgttgtaaa atattaaaaa cgtctctggt tagtgatttc cagaaacgtt
     1321 atcttatcta tttgtttgtt gcttatgaga ggtaaaggag gaacgaatca gcattccacc
     1381 agtgaagaaa acgatagcga tgaccagaag tttcaagcta ttaatatcta aatttgttaa
     1441 gaaaaacaca gctagtaaaa cgccaataat accaccagaa acaattccag caaaaccatc
     1501 ccaactgaca cggtttgctt tgataaactg gctactagaa gctgtcataa tcatagctgc
     1561 atctagcatc ataacaggca tagcaactgt tgggctaaga cccattaatg aaaagaaaat
     1621 caattctgga gcataattgc ctaaccccat agtcatcaag actccaacga taaaattaaa
     1681 accaattcca acaaaaagcc agatgccatg gaggccatga atggtgtctg aaaggtgttc
     1741 tcctggatgg tagattattc taatgatcat gattatagct gctatgatta aaagactacc
     1801 aaggattcgt tggacagttg gagcatgcca gttttttgta atatgagtcc ccaaataagc
     1861 tccaataaag gctgctgcag ccatagcaag taaagttaga acctcaactt ttacgacaaa
     1921 aataaagcaa agagattgaa tgagcaccgg aagcacatga gctacggtca tagtgccagg
     1981 caattgtcta tcgtcagtga ctaatttagt taacttaaag cacgttgtgg tagtagcaaa
     2041 agaccctatc ccaagggtgt caagggcatc tgttagcagg ccaatccaaa accccgtcca
     2101 aaagcgttta aaaggattga tcttattttt tttaagatgt tttactaaca taaagaaaat
     2161 aaaaagcatt gccgaaacta gcaaagcttg tatcagtcta aggataagca tttcgttcat
     2221 aaagactagt ttacaaaaaa aaaccttagc cgtcaacgtg atttacttca ttaaaacatg
     2281 ccactatgct tagaagagct ttggttcgat gtattttgtt aaaattaagc taaaatatta
     2341 atattgcatg aaaggggaca ccttcctttt tcccttactg agcttagctg ttattgtagt
     2401 gaatgtttaa aaggctagtt tgtcgctctt tatcaaaaat ctagtaggat gaatacgtat
     2461 aatttcttgt caaatagagg aattttgtct ataatagcag aggattagga ggaaattatg
     2521 actttaatgg aaaatattac ctttaaaact ccagtattac gcgttaatga tcgcgacctt
     2581 aatattgcat tttatcaaaa taatttaggg cttcgtttag tatctgaaga aaatgcgatt
     2641 gctatctttt catcttgggg agaagggcag gaatgttttg taattgaaga gtcaccttca
     2701 gttagaacgc gtgcggtaga aggccctaaa aaggttaata cgattgtcat caaaataaat
     2761 caacctaaag aaatcgaaca attattagct catggagcac attatgacgc tctttttaaa
     2821 ggtcaaaatg gctatgcctt tgagactatt tccccagaag gagaccgatt cttgttgcat
     2881 gctgaacaag atattaaaca tctccaaggt actgatttac catcgcttga aaaagatgcg
     2941 acttttaaag gtttaactca atttaagttt gatattattg ttttaaatgt tattagtgaa
     3001 gaacgctcaa aagcttttta tcgggatctg tttagtgatc agttacctat tactatggat
     3061 tttattcaag aagaaggtcc cgatttggca attgatcccc atattgcttg ggatttagaa
     3121 attttagaat ttcaagtctc aaaggactat gatatgaagg tcctaaaagc aactttagaa
     3181 gaagacgggc acaaggtcta cattgataaa aaacacaaag tattggtcct ttctgatcca
     3241 agtcagatcg aagtatggtt tacgaaatga ctttggcagt cattaaatgt attgaaaacc
     3301 atcttcacaa aaaagtatac aaaggggcca gtctggctct ctttcaatct ggtcgatggc
     3361 aagaatacca tatcggaaca attgatggaa gacgaccagt tgatgctaac ttagtttatg
     3421 acttggctag tgtttcaaaa gttgttggtg tggccacaat atgcaatatt ttattgaata
     3481 atggtacgtt agcattagat gatcccttaa aagtatatta tcctagtatt gctgatgcga
     3541 ctgtcactat tcgacagtta ttgactcata ccagtggtct tgatccttat attcctaaca
     3601 gagatgtttt aaatgcacaa caattaagaa aagcacttaa tcatcttact caaaaaggaa
     3661 ataaaaactt ttattacaca gatgtcaatt tcctcttgtt aggctttatg ttagaagagc
     3721 tctttagtga atctttagac cagatatttg acaagactat ctttactccc tttgggatgt
     3781 atcatacgtc ttttggccct cgtccagaag ctgtaccaac attaaagggt gtgagtgatg
     3841 gggaagttca tgatcccaaa gcaaaaatct taaaaaaaca ttctggatct gcggggctat
     3901 tttcaactct tgcagattta gaaagtttta gcaatcatta tcttaatgat ccattttcag
     3961 attgtctgtg gcgcaactac agccaacaaa caattgagcg gtcactaggg tggaatttag
     4021 atggcgattg gattagtcat acaggttaca cagggccgtt tctaatgctt aataaaaaag
     4081 agcaaacggc agctattttt ttgaccaacc gtacttacga cgaggatgat aaaagtaaat
     4141 ggttaaaaga acgtcagttg ctttacaatg cactaaagca tgatctgaca acacccgtat
     4201 cgtaacgtct aaaggggcag ttttagtgat cttattactg tttttaaagt ttcttctaaa
     4261 aaatagccat ttcatttgtc tgatg
//
LOCUS       DAUCWU010000108         4176 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUCWU010000108 DAUCWU010000000
VERSION     DAUCWU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.108"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            13..1260
                     /locus_tag="VGY24_001603"
     CDS             13..1260
                     /locus_tag="VGY24_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ4284565.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            1534..2895
                     /locus_tag="VGY24_001604"
     CDS             1534..2895
                     /locus_tag="VGY24_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4284566.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            2895..3731
                     /locus_tag="VGY24_001605"
     CDS             2895..3731
                     /locus_tag="VGY24_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ4284567.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            3918..4160
                     /locus_tag="VGY24_001606"
                     /pseudo
     CDS             3918..4160
                     /locus_tag="VGY24_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1270 a          847 c          715 g         1344 t
ORIGIN      
        1 gaggaaatag ctatgaaatc atggcaaaaa gttatcgtcg gcggagcaag tttgacactt
       61 gcaagtacct tgttagtggg atgtggatca ggttcaaaag ataaaaagga agctggcgct
      121 gactcaaaga ctattaaact ttgggtaccg actggctcta aaaaatcata tgcagatacc
      181 attgcaaaat ttgaaaaaga ttctggttac accgttaaag tggttgaatc agaagatcca
      241 aaagcacaag aaaaaatcaa aaaagatgcc tcaactgctg ccgatgtctt ttcgcttcct
      301 catgatcaac ttggtcaact tgtagaatct ggtactattc aagaagttcc agaaaagtac
      361 aataaagaaa ttgctgctac ttctactgat caggcgctcg tcggtgcaca gtacaaaggg
      421 aaaacgtatg ctttcccatt tggtattgaa tcacaagttc ttttctacaa taaatcaaaa
      481 ttagctgctg aagacgttac ttcatatgat acaattacta caaaagcaac ttttggtggt
      541 acctttaaac aagctaacac ttacgctact ggtccattat tcatgtcagt tggtaacact
      601 ttatttgggg aaaacggtga agatgtaaaa ggtactaact ggggcaatga aaaaggcgct
      661 gctgttctta aatggatcgc agaccaagct tcaaataaag gttttgttag cttagatgct
      721 aacaacgtta tgtctaaatt tggtgacggt tcagtagctt cctttgaatc aggaccgtgg
      781 gactatgaag ctgctcaaaa agctatcggt aaagaaaacc ttggtgttgc tatttatcca
      841 aaagtaacta ttggaggcga aacggttcaa caaaaagcat tcttgggtgt aaaactttac
      901 gcagtcaacc aagcaccagc taaaggtgat acaaaacgta tcgcagctag ctacaaattg
      961 gcatcctact tgactaatgc tgaaagccaa gaaaaccaat tcaaaactcg taacattgtc
     1021 ccagccaaca aagaagttca atcttcagaa gctgttcaat caaacgaact tgctaaaacc
     1081 gttatcacta tgggttcttc ttcagactac actgtcgtaa tgccaaaact tagccaaatg
     1141 ggcacattct ggactgaaag tgctgctatt cttagtgatg ccttcaacgg taaaatcaaa
     1201 gaaagcgact accttactaa gttgcaacaa ttcgacaaag atatcgctgc aacaaaataa
     1261 tatgatctca tgtcatcact gctatactaa aacaagcaca aggtaagtac ttctaaaaag
     1321 ttctttccac ttaccttatg aagagtggaa ctaggctcca tcacatttta ttcaagctta
     1381 gtctcactct tacttgtttt tgttttacta gtagcttagt gctacttaaa tttttaactc
     1441 tttaaaagtt tacgtaacac ttagctattt catcctaaca agagattgaa tctgaactac
     1501 cactctagta tcctaaaata aaggagaatc tatatgactc agtcaaactt gacaccaaat
     1561 atatctgtaa tagaagcctt aaaaagaggc agttgggata ttaaactttc gagtattatc
     1621 atgggatttg ccaactttgc taacaaacag tttatcaaag gaatcttatt tttaataagt
     1681 gaacttatct ttctagtagc ttttgtctcc caaatcatcc ctgctatccg tggccttgtc
     1741 actcttggta ctcaaactca gggaatgact acaaaaacta ttgatgggat taacatccaa
     1801 gttgctgttg atggcgataa ctctatgcta atgcttattt ttggtttagc atcactcatt
     1861 ttctgcctag tctttgctta catttactgg tgtaatctca aaagtgctcg taacctttat
     1921 cttttcaaac aaaaaggtca gaagatacca agcttcaaag aagatcttgc cacacttaca
     1981 aatggtcgct ttcatatgac tttaatggct atcccactga ttggtgtgct tttattcacc
     2041 attcttcctt tgatttacat gatctgtttg gcctttacca attttgatca taatcacttg
     2101 cctccaaaat ctttatttga ttgggtggga cttgctaact ttggaaatgt tttaagcggc
     2161 cgtatggcag gaaccttttt tcctatcttc tcatggactt tgatttgggc tgtttttgcc
     2221 acagtaacta atttcttctt tggtattatt cttgctttgt taatcaatac caaaggatta
     2281 aagtggaaaa aaatgtggcg aaccatcttt gttatcacca ttgcagttcc acaattcatc
     2341 tcattattga ttatgcggaa cttactcaat gacgaaggcc cactaaatgc tcttctcaat
     2401 aaaatcggct tgattaatgg ctcactgcca tttttatctg atcctctttg ggcaaaattc
     2461 tcaatcattt ttgtcaacat gtggatcggt attccattta ccatgttgat tgcgactggt
     2521 attatcatga accttccaag tgaacaaatt gaggctgctg aaattgatgg cgctagcaaa
     2581 ttccaagtct tcaagtctat cacgttccct caaattctct tgatcatgac accaaacttg
     2641 attcaacaat ttatcggaaa tatcaataac tttaacgtca tttacctcct tactggtggt
     2701 ggtccaacaa attcagaata ctatcaggca ggaacaacag acttgctggt cacttggctc
     2761 tataaattaa ccgtcacagc tgctgactac aatttagctt ctgttatcgg tatcttaatc
     2821 tttacagttt cagctatctt tagcttactt gcttatacaa ggacagcatc ctacaaggaa
     2881 ggagcggcta aataatgaaa aacaaacgtc gatttcaact tggacttgtt tatgccactt
     2941 taatcatcct ctcaatcatt tggctcttcc caatcgcttg ggttattctt actagcttcc
     3001 gtagcgaagg aactgcttac gtcaattatt ttattcctaa gacttttaca ttaaatcact
     3061 acattaatct ttttactaat gaaacctttc cttttggaaa atggtttatg aatactctta
     3121 ttgtagccac ttttacatgt attatttcaa ccttcataac tgttgccata gcctactctc
     3181 ttagccgaat taagtttaag tttcgcaatg gctttttaaa actagccctt attttaaata
     3241 tgttccctgg tttcatgagc atgattgcca tctactatat cttaaaggcc ttaggtttga
     3301 cgcaaaccct aacagcactt gtccttgttt attcctcagg tgcagccctt ggtttttaca
     3361 ttgcaaaagg attttttgat accattcctt attcacttga tgaatcagct atgattgatg
     3421 gcgctactcg tatggatatt ttctttaaaa taacccttcc tttagcaaaa ccaatcattg
     3481 tctatactgc ccttcttgct tttatggggc cttggattga ctttatcttt gcacaagtta
     3541 ttttaggtga tgctacaagt aaatataccg ttgctattgg actattttca atgcttcagc
     3601 cagataccat taacaactgg tttatggcct tcactgctgg ttctgtctta attgccgtcc
     3661 caatcactct cctcttcatg tttatgcaaa aatactatgt tgaaggcatc acaggtggtt
     3721 ctgttaaata agttacaaca taccaaacct tctaagggca agccctttag gaggtttttg
     3781 ataaataagg ccgtagataa tcatattagt cctcacattt atcttcagat cccgcgttat
     3841 aaaatgaagt acaacaaaaa agacatgttc gtctgatata ctagaattcc ccaattcagt
     3901 atggaaagca gatgaacatg tctaatatta attctaccag aaaatcatcc tattctcatc
     3961 tttcagccac agaacgcggt gaaattgctg cttatcttaa aatgggaaag aaacccgttg
     4021 agattgctcg acttctgggt tgtcaccgtt ctacaatctg ccgagaaata aaacgtggtt
     4081 cagtagatca ggtaaaggat aagaatggaa aacaaacctt cttcaacgcc tattttgctg
     4141 atagtgggca acgtgtctat taaaccaatc gatttg
//
LOCUS       DAUCWU010000109         3994 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUCWU010000109 DAUCWU010000000
VERSION     DAUCWU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.109"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            366..1433
                     /gene="pepA"
                     /locus_tag="VGY24_001607"
     CDS             366..1433
                     /gene="pepA"
                     /locus_tag="VGY24_001607"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ4284568.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            1481..2251
                     /locus_tag="VGY24_001608"
     CDS             1481..2251
                     /locus_tag="VGY24_001608"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ4284569.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYVMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(2334..2642)
                     /locus_tag="VGY24_001609"
     CDS             complement(2334..2642)
                     /locus_tag="VGY24_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284570.1"
                     /translation="MYVFIEPNDLKDYLITIYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLPLIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(2828..>3994)
                     /locus_tag="VGY24_001610"
     CDS             complement(2828..>3994)
                     /locus_tag="VGY24_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ4284571.1"
                     /translation="SSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQI
                     LDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVVVNDKVLEQIE
                     ELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLYPIPQKYYTDY
                     KVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGF
                     TPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRD
                     IEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGENAPLMRQDVI
                     GGLSWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK"
BASE COUNT         1238 a          886 c          750 g         1120 t
ORIGIN      
        1 aaactaaaag tacattgatt aggtgtggtt tattattaat ttaaccatac tataaaaatc
       61 gctgattaac ctaaaatgga aggttctcct tatgactcca ttatccttac ttttcgctag
      121 tgaagagtct tttcgcatcc taagcgccct actattctta ttaaccagct tgtgctttat
      181 cattttaatg atttccttgc tgcctaaaaa aacaggttat cgccaaaaga caaacacttc
      241 ttccagagat ctctagtctc tgggattttt ctttgctgtg aaacccattc ttacttaaaa
      301 agtggtagaa tatagggaga tgaccgcgtt gaactggtct gaaaccttgt aaatggagat
      361 gaaaaatgac agacttattc tcaaaaatca aagaagttac cgaactggat ggcattgcgg
      421 gctatgaaca tagcgttcgt gactacctac gcaccaaaat aaccccgctg gttgaccgtg
      481 ttgaaacaga cgggcttggt ggcatttttg gtatcagaga tagtaaagct gaaaaagccc
      541 cccgtatttt agtagctgcg cacatggacg aagtcggttt tatggtcagt gatatcaaag
      601 ttgacggaac gctacgcgtg gttggtatcg gtggttggaa cccacttgtt gtcagttcac
      661 aacggtttac cctttacaca cgcactggcc aagttattcc ccttatttca ggatcggtac
      721 ctccccattt tttacgtggg gcaaatggct ctgctagtct accaaatatc gaaaatattg
      781 tgtttgatgg tggctttacg gataaggcag aagctgaaag ctttggcatt acaccgggtg
      841 atattattgt ccctcaatct gaaacgatcc taacagccaa tcaaaaaaat attatttcaa
      901 aagcttggga caatcgctat ggcgttctca tgataacaga aatgcttgaa gcgttaaaag
      961 gacaagacct taacaacacc ctaattgcag gtgctaacgt tcaagaagaa gttggtctgc
     1021 gcggagccca cgtctcaacc accaagttcg accctgaact ctttttcgca gtagattgtt
     1081 cgcctgctgg tgatatttat ggcaatcctg gaacaatcgg ggatggcacc ttgttgcgtt
     1141 tctacgaccc aggccatgtc atgctcaaag atatgcgcga cttcttactg actactgctg
     1201 aggacgctgg tgtcaatttc caatactatt gtggcaaggg aggcacagat gcaggtgctg
     1261 cacaccttca aaatggtggt gtcccatcaa caaccatcgg agtctgtgca cgctacattc
     1321 actctcatca aaccctctac gctatggatg atttcgtaga agcccaagcc ttcttacaag
     1381 ccattatcaa aaaactggat cgctcaaccg ttgacttgat taaacgttac taaactagca
     1441 ttagcctgat taaccataat atcccacaag gagcaacaga atgaaaattg gcattattgg
     1501 tgttggcaaa atggctagcg ctatcatcaa aggccttaaa caaacacccc atgaacttat
     1561 catttcagga tcatctttag aacggtccaa ggaaattgcg gagcagttaa cactgcctta
     1621 tgttatgtcc caccaagacc ttattgacca ggttgatctt gttattttag gcatcaagcc
     1681 tcaactattt gaaacggtac tcaaaccgct tcacttcaaa cagcctatta tatctatggc
     1741 agcaggcatt tcccttcaac gactagcaac attcgtagga caagaccttc cgctgctacg
     1801 tatcatgcca aacatgaatg cacaaattct ccaaagcagt accgctttaa cgggaaatgc
     1861 tttggtgtcc caggaattac aagcacgtgt tcgagactta acagatagct ttggtagcac
     1921 atttgatatt agtgaaaagg attttgacac ctttaccgct ttagcagggt caagtcctgc
     1981 ctatatttat ctctttattg aggctttggc taaggctggc gtcaagaatg gcatgcctaa
     2041 agcaaaggcg ctggagattg ttactcaaac agtcttggct agtgccagca atctcaagac
     2101 cagttctcaa agtccgcacg atttcattga cgctatttgt agccccggtg gcacaactat
     2161 tgctggtctg atggagttag aacgccttgg cctcacaact actgtcagct ctgccattga
     2221 caaaaccatc gataaagcta aaagcttgta aaataagtgc cccaatacaa atcagacaac
     2281 aatacaaaca cctccgttga ccagacagga aactcaaccg ttagacaaaa catctaacaa
     2341 agaacctgac tcgtatcaaa cggaggtttt tgactattag ttgttatttt ccctagtgcc
     2401 tccattttct tagccaccag acttctgata atcaagtaaa ggtaagcaaa aatcaaaggt
     2461 agcttataag aaacaagatc tcgaatggtt aagcaaggga agaaaaagaa taaggcaaca
     2521 aatagaacaa tcatcaggta aaaacagtaa acttgtgtct gttttcgaat cgcacgatta
     2581 cgctcatcat aaatcgttat caaatagtct tttagatcat tgggctctat aaacacatac
     2641 aagaaaagcg ttgaagcaat taaaaactct aaataaggct gacatccaaa tcgcctagca
     2701 caagattaca aaaagcaagg agactgatca gaactaatac ctaggcacca taactaacag
     2761 ataaacctca taaaaaacca agtaaagtgt tgccttactt ggttaatcta ttgagactta
     2821 gtctctttta ttttgtattt ttcaaacgct caacgtcacg cgcgatgcac aattcttcgt
     2881 ctgttgagat aacaagaacc ttcacttttg actcaggtgt tgaaatatca ccacggtatc
     2941 caaaaacatt tttctcagga tcaatgtcca tgccaaacca tgacagacca ccaataacat
     3001 cttgacgcat caatggtgca ttttcaccca taccagctgt aaagaccaaa gcatctgctc
     3061 cattcaaaac agcaaaatat tgaccaatgc attttttaat gcgatcaata aagatattgt
     3121 aagctaaaac agcatctgga ttatcttctt gtaaaccagc ctcgatatca cgcatatcac
     3181 tagaaatgcc tgacacccca ctaagacctg atttcttatt taacatattc acaacatcag
     3241 cagcgtcttt taattctggg tcttgctcaa ttaaataagg aatgatagct gggtcaatgt
     3301 caccagaacg tgttcccatc ataggaccag caagtggtgt aaagcccatc gaggtatcaa
     3361 ctgatttacc gtggtaatta gctgtaattg agacaccatt tccaatatga gcagtaatta
     3421 gttttaattc ttctaatgga cgaccaagca ttttagcagc ttcttgcgct acatatttat
     3481 gacttgtccc atgcgcccca tatttacgaa ccttgtagtc cgtataatat ttttgaggaa
     3541 ttgggtaaag gtaagtatgc tttgccatgc tagtgtgaaa tgacgtgtca aacacacaaa
     3601 cactggtaat atctggcaaa atatcacgga aggcacgaat ccctgccgcc gctccaggat
     3661 tgtgaagtgg agctaaaacg gataattctt caatctgttc aagaactttg tcattaacca
     3721 caacggattc tttgaaaagt tcaccaccag caacaacacg gtgtccaact cctgtaattt
     3781 catcataagc tgcaatgatt ccaaagtgaa tcaaatcgtt caataagatt ttaacagctt
     3841 ctgtatgatc gtgaatatca agaatttgct cttctttttt gccatcgtac ttaactgttg
     3901 aaatagaatc cttgagaccg atacgctcaa taattccttg tgctaacact gcttcttctg
     3961 gcatctgata aagttgccat tttaaactag aact
//
LOCUS       DAUCWU010000110         3950 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUCWU010000110 DAUCWU010000000
VERSION     DAUCWU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.110"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..97
                     /locus_tag="VGY24_001611"
     CDS             <1..97
                     /locus_tag="VGY24_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEQ4284572.1"
                     /translation="KQANIIVTNRYDNELQDVKNKVYSRDIFNRD"
     gene            279..1193
                     /gene="hasC"
                     /locus_tag="VGY24_001612"
     CDS             279..1193
                     /gene="hasC"
                     /locus_tag="VGY24_001612"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEQ4284573.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGSGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PQVKEDLKNYIIKLGKALEKSKVPTHSK"
     gene            1501..1914
                     /gene="yaaA"
                     /locus_tag="VGY24_001613"
     CDS             1501..1914
                     /gene="yaaA"
                     /locus_tag="VGY24_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ4284574.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIITIVEPSQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKIKGKLTAPVRFPGI"
     gene            1916..3022
                     /gene="recF"
                     /locus_tag="VGY24_001614"
     CDS             1916..3022
                     /gene="recF"
                     /locus_tag="VGY24_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ4284575.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(3078..3941)
                     /locus_tag="VGY24_001615"
     CDS             complement(3078..3941)
                     /locus_tag="VGY24_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ4284576.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT         1382 a          756 c          687 g         1125 t
ORIGIN      
        1 gaaacaagca aatattatcg taactaatcg ctatgataat gaattacaag atgttaaaaa
       61 taaagtttac agtagagata tttttaatag agactagcca tgtcttcatc tataacaata
      121 atattcattt ttactttaaa agaactgcag ttggtggtat gattgagcac tatgaaatag
      181 tcgaaaaatc atatgaaaac gtaacatgat gtcaaatgaa ttccgttctg aaaacgaatg
      241 tcactaagcc atagattata aaaagtgagg agttactgat gaccaaagtc agaaaagcca
      301 ttattcctgc tgcaggtcta ggaacacgtt ttttacctgc taccaaagct cttgccaaag
      361 agatgttgcc catcgttgat aaaccaacca tccagtttat cgtcgaagaa gcgctaaaat
      421 ctggcatcga ggaaatcctt gtggtgaccg gaaaagctaa acgctctatc gaggaccatt
      481 ttgattcaaa ctttgaatta gaatacaacc tccaagctaa ggggaaaaat gaactgttga
      541 aattagtgga tgaaaccact gccattaacc ttcattttat ccgtcaaagc cacccaagag
      601 gactgggaga tgctgtctta caagccaaag cctttgtggg caatgaaccc tttgtggtca
      661 tgcttggaga tgacttaatg gacattacaa atgcatccgc taaacctctc accaaacaac
      721 tcatggagga ctatgacaag acgcatgcat ccactatcgc tgtgatgaaa gttcctcatg
      781 aagatgtgtc tagctatggg gttatcgctc ctcaaggcaa ggctgtcaag ggcctttaca
      841 gtgtagacac ctttgttgaa aaaccacaac cagaagatgc gcctagtgat ttggctatta
      901 ttggtcgtta cctcctaacc cctgaaattt ttgatatttt ggaaagacag acccctggat
      961 caggtaacga agtgcaactc acagatgcta tcgataccct caataaaact cagcgtgtct
     1021 ttgcacgaga atttaaaggc aatcgttacg atgttgggga taaatttgga ttcatgaaaa
     1081 catctatcga ctatgcctta gaacacccac aggtcaaaga ggacttgaaa aattacatta
     1141 tcaaactagg aaaagctttg gaaaaaagta aagtaccaac acattcaaag taatgtgttg
     1201 gtactttact tttttccttg tttatagcaa cttaagttta catcttgaaa attgtcaacg
     1261 ttccaaaata taaaagcttt gtgttataca actttactag agggacatat taataatagg
     1321 atttatacgt agaaaaatga taaacataag tacagctttt gttaaaacta attaacaaga
     1381 acttcaaaac taaatagtta tttggacgag tatgatactt tataaatagc tcttattcag
     1441 ctattattat ttatttctgt tttatgctat aatgacttga atgacaaaat ggagatgaca
     1501 atgatataca aattatttac agaatttatc accctacaag cccttcttaa agaattagga
     1561 attatccaaa gtggtggcgc tatcaaggga tttctcgctg aaacaaccgt tctatttaat
     1621 ggtgaagatg agaaacgtcg aggcaaaaaa atcagagtag gggataagat ttctctacct
     1681 gaccaagact taattatcac tattgttgag cctagtcaag aagaaaaaga acaatttgcc
     1741 gaagaaatgg ctgaaaagac acgtgtagcg gctctagtga aacaaatgaa ccaagctaac
     1801 aaaaagactt cttctaaaca taaaaatcgt caatctacta ccaaaaaatc actaagagca
     1861 actaagaaaa ttaaaggtaa actaactgca cctgtccgct tcccaggtat ctagtatgtg
     1921 gattaaagag ttagagttaa aacattatcg caattatgac cacctgcttg cctcattttc
     1981 gtcgggttta aatgtcttta tcggtaataa tgctcaagga aaaaccaatt ttctcgaggc
     2041 aatctatttt ctatcactta ctagaagtca tcgcacgagg gctgataaag aattgattca
     2101 ttttgatcac tcaactgttt ctcttactgg taagattcag cgtattagtg gtactgttga
     2161 tttagaaatc aatctatcag ataaaggacg tgtgacaaag attaatgcgc taaagcaagc
     2221 aaaattatct gattatattg gaacgatgat ggttgtgctt tttgcaccag aagatttgca
     2281 actagttaag ggagcaccta gccttcgtcg caaatttatc gacattgacc ttggtcaaat
     2341 taagcctgtt tacttatctg agttatccca ttataaccac gtgcttaaac agcgtaatag
     2401 ctatttaaaa agtgcccaac aaattgatgc cgcttttcta gctgttctag atgagcaatt
     2461 agctggctat ggagcccgtg tcatggaaca tcgtattgat tttatcaatg cgttagagaa
     2521 agaagcaaat actcatcacc aagccatctc aaatggttta gaaagtctct ctctctccta
     2581 tcagtcgtca gttgtctttg acaaaaaaac taatatttac caacaatttt tacaccaact
     2641 tgaaaaaaat catcagaaag atttcttccg taaaaacact agcgttggtc cacatcgcga
     2701 tgacttagcc ttttacatta acgggatgaa tgctaacttt gctagtcaag gtcagcaccg
     2761 cagccttatt ctttccttaa aaatggctga agttagttta atgaaggcct tgactggaga
     2821 taatcccatt cttttactag acgatgtcat gagcgagcta gacaatacac gacaaacaaa
     2881 gttacttgaa acagtaatta aagaaaatgt ccaaaccttt atcacaacaa caagtttgga
     2941 tcacctgtca cagttgccag aaggtatccg tatttttcac gtgacaaaag gaaccgttca
     3001 aattgattct gatattcact aataacatca aaaaagggtt cagttaaact gagccttttt
     3061 ttgatattat gtggcgatta agatttgacc acacctaata aaatagcacc tacgataaaa
     3121 caaataattc ctgtgacaac ccaacgcatt tcttttttgg ttttggtttc gcctaggaat
     3181 aaaatgcctc cgacaatcga aatgatagca cctaattgtg agaaactaaa ggcaatagca
     3241 agtcctgctt ttgatgcagc taaaagcata aaaatattcc caatgcccca aagtaatcca
     3301 acaacgctat ttttaatcac atactggtca atagaaattt taaatgacat gaaagttata
     3361 gctcctaaaa ccatacctac tgccatgggt aaaatgactg acaaaacttc aaatttcatg
     3421 atgttattaa ataatacagc atacatcacg taaccaattg ttgaataagt gagcgcccta
     3481 aatccttttg aaaaattatg aaggtgattg acttgtgcat tagcatcatc ttgtttacta
     3541 gaaaagtaaa aaccaacaat cagaagtaat agtgctagac ttcctactac aaattgcata
     3601 ggtcttgtcc attcatgaaa aacgagcaca ccaatcaaac ttcccagaac aagttgagag
     3661 ccacttgaca aaggattggc aacggagact cccatgtatt gcatggcatg aaattgacca
     3721 gtttgaccaa ttgaccagat aaaaccaccc aatattccaa aaagccaaag ttgcagtgtc
     3781 atctcaggtc taacaattaa ccaaacagct aatgaaaata ataaagcccc aaaagtcatt
     3841 cctagagttt gttgcgaagg ttttcctcca attttgttac taacaaatcc aatgcttccc
     3901 catgtaaaca tgggaataag tgcataaaaa ataccttcca aaatgttatc
//
LOCUS       DAUCWU010000111         3939 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUCWU010000111 DAUCWU010000000
VERSION     DAUCWU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.111"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..484
                     /locus_tag="VGY24_001616"
     CDS             <1..484
                     /locus_tag="VGY24_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEQ4284577.1"
                     /translation="NITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGKIPIF
                     GICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLPDT
                     LMVTHEDINDKTVEGVKHRDFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK
                     "
     gene            730..3906
                     /gene="carB"
                     /locus_tag="VGY24_001617"
     CDS             730..3906
                     /gene="carB"
                     /locus_tag="VGY24_001617"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ4284578.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
BASE COUNT         1273 a          650 c          833 g         1183 t
ORIGIN      
        1 caacatcact gcagaagaag tgctccaact aaatccggat ggcttgatgc tatctaatgg
       61 cccaggaaac ccagaagatt tgccagaggc tttagacatg attcgtgggg tacaaggtaa
      121 gattcctatt tttggtattt gtatgggaca tcaactcttt agtctagcaa atggtgctaa
      181 gacttgtaaa atgacctttg gtcatcgtgg ctttaaccat gccgttagag aaattgcaac
      241 gggaagaatt gattttacca gtcaaaacca tggttatgcc gttgaacgta gtagtctacc
      301 tgatacttta atggttacgc atgaagacat taacgataag acggtcgaag gagtgaaaca
      361 tagagatttt ccagcttttt cagtccaatt tcaccctgat gcggctccag gacctcatga
      421 tgccagctac ttatttgacg aatttttgga aatgattgat tcttggagat gtacgagtaa
      481 atagtaaatc agttgcaatt tcacgactat atcacctctt gttactctat ttattaacat
      541 ttacagttga tttagtaccc tatcaaaaat attatttaat gttgctctgt gaggcaacaa
      601 atagagaaag gaaggcaagg agaacacaag taaataattt agtaaaaagt taggttttca
      661 aagagataaa gctcttattg agaagtgctg actttaccgc ttcttgtggt attcttggta
      721 tcattaatta tgccaaaacg aaaagatatt caaaaaatca tggttatcgg gtctggcccg
      781 attattattg gacaagctgc tgaatttgat tatgcaggga ctcaggcctg tttggctcta
      841 aaagaagaag gctacaaagt catacttgtt aattcaaatc ctgcaacgat tatgacagat
      901 aaagaaatcg ctgataaggt atatattgag cctttgactc ttgaatttgt taatcgtatc
      961 attcgaaaag aaaggccaga tgccatactg ccaaccttag gtggtcaaac gggcctcaat
     1021 atggcaatgg cattatctaa agctggtatt cttgatgatt tagagattga attgttaggt
     1081 actaagttat ctgcgattga tcaggcagaa gacagagacc ttttcaaaca attaatgcaa
     1141 gaactagacc aacccattcc agaatctaca attgttaaaa cagttgatga agctgttaca
     1201 tttgctagag atatcggtta tccagtcatt gtgcgcccag cttttactct tggtggaact
     1261 ggtggaggaa tatgtagtag tgaagaagaa ctttgtgaaa ttactgaaaa cggcctaaag
     1321 ttatcaccgg tcacacagtg tttgattgaa cgctcaattg ctggcttcaa agaaattgaa
     1381 tacgaagtaa tgcgagatag tgctgataac gcgctggttg tttgtaatat ggaaaacttt
     1441 gatcccgtag ggattcacac aggagattct attgtatttg caccaacaca gaccctttct
     1501 gatattgaaa atcaaatgtt acgtgatgct agtttaaaaa taattcgagc tctgaaaatt
     1561 gaaggtggct gtaacgtcca gttagcacta gatccttata gtttcaaata ttatgttatt
     1621 gaagtaaatc ctagagtgtc tcgttcctct gcccttgctt caaaagcaac aggttatccc
     1681 attgccaaat tggctgcaaa gattgctgtt ggcctaacac ttgatgaaat gattaaccct
     1741 atcacaggca ctacttacgc tatgtttgaa cctgctcttg attacgttgt tgctaaaatt
     1801 ccgcgttttc cgtttgataa atttgaacat ggtgaacgcc aactgggaac tcaaatgaaa
     1861 gcaacaggtg aagtcatggc tattggccgt aatttggaag agtccttact aaaagcctgt
     1921 cgttctttag aaattggtgt ttgccataat gagatgacaa gtttaagtaa cattagcgat
     1981 gaagagttag tgactaaagt gattaaagct caagatgatc gactttttta cttgtcagaa
     2041 gcaatacgac gaggttatag tatcgaagaa cttgagagct tgaccaagat tgatttattt
     2101 ttcttggata aattacttca tattgttgaa atagagcaag aattacaaat gcatgttgac
     2161 catttggaaa gcctcaaaaa agcaaagcgt tatggatttt cagatcaaaa aattgcagaa
     2221 atttggcaaa aggatgaaag tgatatcaga gcgatgagac attctcatag cctgtatcct
     2281 gtctataaaa tggtagatac ttgtgcagct gaatttgatg ctaaaacccc ttatttttat
     2341 tcaacatatg agttggaaaa tgaatctgtt cagtcgaata aagaatcaat tttagtttta
     2401 ggttcaggcc ctattcgtat tggtcaaggg gttgaatttg actacgcaac ggttcattct
     2461 gttaaagcca tccaaaaagc tggctatgaa gccattatca tgaattcaaa tccggagact
     2521 gtatctacag atttttcggt atcagataaa ttatattttg aaccgttaac ctttgaagac
     2581 gtcatgaatg tgatagattt agagcagcct aagggagtaa tagttcagtt tggaggacaa
     2641 actgctatta acttagcaca agcactttct gaggcgggtg ttacaatatt aggaacacaa
     2701 gtagaggatt tggatagggc tgaagacaga gatttatttg aaaaggcgtt gaaagagctt
     2761 ggaatcccac aaccacaagg tcaaacggca actaatgaag aagaagcttt agaggcagct
     2821 aaaaaaattg gttttccagt cttagttaga ccttcttacg tacttggtgg ccgggctatg
     2881 gaaattgttg aaaacaaaga agatcttaga gaatatataa gaacagcggt taaagcaagt
     2941 cctgaacatc ctatcttagt ggactcttat attttcggta aggagtgtga agtggatgcc
     3001 atttcagatg gaaaatcagt cttaattcct ggtattatgg agcacatcga aagagcaggt
     3061 gttcattcag gagattcaat ggcagtttat ccgccacagc aattatctaa gcaaattcaa
     3121 gaaacaatag ctgagtatac taaacgttta gccattgggt taaattgtat tggtatgatg
     3181 aatgttcaat ttgttattaa aaatgaacaa gtttatgtta ttgaagtgaa tccacgcgct
     3241 agtcgtaccg tacctttctt atcaaaagta acaggtattc cgatggctca gattgctact
     3301 aagttgattt taggtcaaac attaaaggat ctaggatatg aagatggtct ttatccccaa
     3361 agtcaactgg tgcatatcaa agcgcctgtg ttttcattta caaagttggc acaggttgat
     3421 agtcttttag gtcctgaaat gaaatcaact ggagaagtaa tgggttcgga tactagtctt
     3481 gaaaaagcac tttataaagc gtttgaagcc aataattctc atttatcaga atttggtcaa
     3541 attgtcttta ctatagcaga tgatagtaag gcagaagctc taagtctagc aagacgattt
     3601 aaggcgattg gctatcaaat tatggctact caaggtacag cagcttactt tgctgagcaa
     3661 ggcttaagcg cgtgtttagt tggaaaaatt ggtgatgctg ccaatgacat cccgacactc
     3721 gtgcgacatg gtcacgttca ggcaattgtt aatacagttg gaattaaacg cacagctgat
     3781 aaagacggac agatgattag aagttcagct attgaacaag gagttccttt atttacagct
     3841 ttagatactg caaaagctat gttaacggtt ttagaaagca gatgctttaa catcgaagcg
     3901 atataaaaat taaaaaatca tttttaagaa aacttaaaa
//
LOCUS       DAUCWU010000112         3935 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUCWU010000112 DAUCWU010000000
VERSION     DAUCWU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.112"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..314
                     /locus_tag="VGY24_001618"
     CDS             <1..314
                     /locus_tag="VGY24_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284579.1"
                     /translation="KKKISAVLEKVGLSSDYLNQKIYELSGGQAQRVAIARMLMKPRK
                     IILADEPTGALDGEIGKEIINLLLSETAENKYVIIATHDPAVYNKVDFIIDMKDIGSK
                     K"
     gene            311..802
                     /locus_tag="VGY24_001619"
     CDS             311..802
                     /locus_tag="VGY24_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284580.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPVVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGVVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            1006..1536
                     /locus_tag="VGY24_001620"
     CDS             1006..1536
                     /locus_tag="VGY24_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ4284581.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            1585..2172
                     /locus_tag="VGY24_001621"
     CDS             1585..2172
                     /locus_tag="VGY24_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ4284582.1"
                     /translation="MQHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKITAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIGLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            2324..3052
                     /locus_tag="VGY24_001622"
     CDS             2324..3052
                     /locus_tag="VGY24_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ4284583.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     EIAVKILDRLRKHDPQSLDFLMDISLSMKNQKAWKKRFITLIVCLTVETLLFVGGAYF
                     LQLTVWKYLLIPVFLLVLLGTLYWYYQNICYLCPSCHRTFEAKWTEFALAGHTPRTRK
                     LTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(3109..3408)
                     /locus_tag="VGY24_001623"
     CDS             complement(3109..3408)
                     /locus_tag="VGY24_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ4284584.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(3601..>3935)
                     /locus_tag="VGY24_001624"
     CDS             complement(3601..>3935)
                     /locus_tag="VGY24_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284585.1"
                     /translation="LRLKKNMEQLELANLLGRKSSSSISEWEKGKYTPKSGLLSDIAA
                     IFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTKWVDFAQILLEQQAK
                     EDRELDNH"
BASE COUNT         1297 a          680 c          797 g         1161 t
ORIGIN      
        1 acaaaaaaaa aatatctgct gtacttgaaa aagtagggct ctctagtgac tatttaaatc
       61 aaaaaatata tgaattgtct ggagggcagg ctcagcgtgt agctattgca agaatgttaa
      121 tgaagccacg taaaataatt ttagcagatg agccaacagg agctttggat ggtgaaattg
      181 gaaaagaaat tattaatttg ttgttgagcg aaacagccga aaataaatac gttattatag
      241 caactcatga tccagctgta tataataagg ttgattttat aattgatatg aaagatatag
      301 ggagtaagaa atgaaaaaga aaatttatat tggcttgatt gtcattgttg gagtttttgt
      361 agtgttcttg tctggaaaac aaatcacaca aaaagaaact tcaaataagc ctgtggtgac
      421 cctaacagtt tataccatct cctctagtga tacccaaaaa tggagtaaag taaagcagtt
      481 tgaaacgaaa gaagcaatat acctagtaaa ggtaaaagaa actacctctt cagaagaagt
      541 cttttcaaac atcattgcag atggaatggc agttggtttt ggagttagtg aagaagacgt
      601 taaacagttt aacgataatt tatgtagccc ggtagaagat gctaagaata ataaattaat
      661 tggaatagaa ttcatcaatt tttctagtgt tgataagggg gttgtggttg caaattttga
      721 ctacggcaaa aaagcgttta attctcagaa aacagaaaat aaaaaattat ataaaaagct
      781 ctatgaagcg tataaaggat agctatttat agtttcttga gagtgatacc acttctgaat
      841 aataaatgaa ctaacttggc ataccgtttt ctattcatct aacaatgttt tcaagaaata
      901 attgatgcct ttagcacgac tgaaatgagg tcaaattgac agaatactcc tattatggta
      961 ctatttttaa taagaatatt cagtgatagt gataaagagg aaaatatggg aattgaaaag
     1021 acagttagtg aactagctga tattttggga gtgagtcgtc aggccgtcaa taatcgtgtt
     1081 aagtctttac ccgaagaaga tcttgacaaa aatgaaaaag gcgttactgt ggttaagcgc
     1141 agtggtcttg ttaagctgga agagatctat aaaaaaacaa tttttgacga tgagcctatc
     1201 agtgaagaaa cgaaacaacg tgagctttta gagattcttg tggatgagaa gaacactgaa
     1261 atcacgcgcc tttatgagca actcaaagcc aaagatgctc aacttgcctc aaaagatgag
     1321 caaatgcgtg ttaaagacgt gcagatcgct gaaaaagata aacaattgga tcagcagcaa
     1381 caattaactg ctaaagccat ggctgataaa gaaaccctaa aactagaatt ggaagaagct
     1441 aaagcagaag ccaaccaagc tcgcttacaa gtcgaagaag ttcaggctga agttggccct
     1501 aaaaaaggct ttttaacccg cttgtttgcg aaataatcac aaaaaatgac gaaggcccta
     1561 tcagggtctt ttttggagga aaacatgcaa cacttagata cttatattgc ctttgattta
     1621 gaatttaaca ctgtcaatga cgttagtcat gtcattcaag tatcagcagt caaatatgac
     1681 caccataaag aagttgacag ctttgacacc tatgtttaca cggatgttcc cttacagagc
     1741 tttatcaatg gcctaacagg aatcacatct gacaagatta ctgcggcacc aaaagtagag
     1801 gaggttatgg ctgcgtttaa aaattttgtg ggagagttac cactgattgg ttataatgct
     1861 caaaaatctg acctgcctat tctagctgaa aatggcttgg atttaaggga ccaataccag
     1921 attggcctct ttgatgaagc ttacgataga cgtagcgcag acttaaatgg gattgctaac
     1981 cttcgtctgc aaacagtggc aaccttttta ggcattaagg gtcgaggtca taatagctta
     2041 gaagatgcca gaatgacagc agcaatctat aagtcttttt tagaaacaga tacaaacaaa
     2101 gcatatctca gccagcaaga agaggtaacc acagataacc ctttcgcagc tttgggagac
     2161 tttttcgact aaaggctctg ctatttttga aaaataaaaa aatccctagg gttagccctt
     2221 gcttgtaacg ctaggtcatg tgataagcta ggggcatatc aagagggagt cgggtgcaca
     2281 cgagtcgctt ttgatcacta aatgatagaa agaaggtgtg gaaatgtctc aattctattc
     2341 gactggagaa ttggccaact tagctggcgt ttctattcga actgtccaat actatgacca
     2401 acgtgggatt ttgattccga cagcattgac agcaggtgga cggcgactct atacggattc
     2461 tgatttagaa caattacgca tgatttgctt tctgcgcgat ttgggatttt ccattgagca
     2521 gattcgcaaa gtcttagcag aggaaaatgc agcccaggtg ctggaattgc ttctagttga
     2581 tcatattgcg actgccaagg aagacttggc cgcaaaagag caacaagttg aaatagctgt
     2641 caagatactt gacagacttc gaaaacatga cccccagtca ctagattttc tgatggacat
     2701 ttcgcttagc atgaaaaatc aaaaagcttg gaaaaaacgc tttattactt taattgtctg
     2761 tttgacagtg gaaactctac tttttgtggg tggagcctat tttctccaat tgacagtctg
     2821 gaaatattta ttaatcccgg tgttcctttt agtcttacta ggaaccttat attggtatta
     2881 ccaaaatatc tgttatcttt gtccttcttg tcatcgaaca tttgaagcaa aatggacaga
     2941 atttgccctt gcaggccata caccacgaac cagaaagtta acctgctccc attgccatca
     3001 aaaatcgtat tgcttggaac tggcaaaaga ggacactgtc tcaagaggat aagcactaac
     3061 tcataaaaaa actagcctga gatccttctc aagctagttt ttgttttttt agaatggtaa
     3121 tttaccagca aaagcgccta atgttttctt agttgcttca tcaatttgcg taagggcatc
     3181 attaatggct tgagtggtca tgtcttgaag cgtttctaca tcttcaggat ctacaacagc
     3241 ttctttgaag tcaatcccaa ccaatttttt atcacccgtg aaagtggcag tgacaaggtc
     3301 ttgagctgat tttccagtaa attgcatagc agccaaatcc gcctgctttt gctccatttg
     3361 tttctgaagc ttttgtgctt gcttcatcat gttttgcatg ttcatcataa gactatactc
     3421 tccttaattt aatactaact attataacac ataaaaagag gcaataaaaa caatgtcagt
     3481 tagtagacta gcctgtatca gtaagaaagt cgctatggat aagatggatt agcagcaagg
     3541 gtctggctaa tgacagaata agtctagaaa tggactattt ttgtcataga ctagtccgac
     3601 ttagtggtta tctaattcac gatcttcttt agcttgttgt tctaaaagaa tttgtgcgaa
     3661 gtcaacccac ttagtgtagt tattttcgga taattgttct gaaatcatcg ctaactctcg
     3721 ttgtggcaac tgataagaga ctcccttact ttctaaggtt aaatcttctt ccattagggc
     3781 ggttaaacta acatcaaaga tagcagcaat atcacttaat agaccagatt ttggagtata
     3841 ttttcctttt tcccactcac tgattgaaga agagcttttt cgtcctagga gattagcaag
     3901 ttctaattgt tccatgttct tttttaatct aaggt
//
LOCUS       DAUCWU010000113         3825 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUCWU010000113 DAUCWU010000000
VERSION     DAUCWU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.113"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..334
                     /locus_tag="VGY24_001625"
     CDS             <1..334
                     /locus_tag="VGY24_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEQ4284586.1"
                     /translation="PPFTVTNTMLNKVVEISKIIGNLELQVQKDLKLRKENRIQSIHS
                     SLAIEQNSLTVEQITAIIDGKRVLGNPREIREVKNAYEAYEEILTLTPYDESHFLKMK
                     EFQQYIYR"
     gene            358..1182
                     /locus_tag="VGY24_001626"
     CDS             358..1182
                     /locus_tag="VGY24_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine ligase"
                     /protein_id="HEQ4284587.1"
                     /translation="MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYL
                     MPTFRGEAVDHRHLSQFYHSECEIEGDLHDVMNLVDRYIKFLVSEILRECRDSIFAIT
                     KDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
                     VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQ
                     VDASEYSWYLEMKKTRPMKSSGFGLGVERLIAWILKCDDVRKVQLISRENGIDYLP"
     gene            1196..2443
                     /locus_tag="VGY24_001627"
     CDS             1196..2443
                     /locus_tag="VGY24_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HEQ4284588.1"
                     /translation="MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFV
                     ETIETSFRNFGKTIEQIKELKYSIDAVACWDEALTHIADDISKELGLNPISSLDSQSF
                     RFKDRMRMVCEAGGLKMPKYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNF
                     SELQQAVSQMLNVKFPVYIASGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIY
                     TPLGITKKIVDEKLFMDEIGHIFPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFR
                     IGRNGDIILIEIGARIGGDCIPNLMKNSFIVKGFDFYNTYLKARLGLQEELNTDTNKF
                     SGIVFFRLNPENYGKIFNGVSYDAPKTSLIFEEKIYKKEGTVLPNPINWSNERVGYVC
                     LENKEYAVLEKEMKEILKSLSIE"
     gene            2368..3387
                     /locus_tag="VGY24_001628"
     CDS             2368..3387
                     /locus_tag="VGY24_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEQ4284589.1"
                     /translation="MFGKQGIRCARKGDERNIKIIVNRVNSERNIKSIGICSPSYPGT
                     FHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGEISGGVQDRAEELNQLIPRVDVIM
                     TSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNF
                     GEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFN
                     KGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGI
                     FDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKI
                     TLSLS"
     gene            3400..>3825
                     /locus_tag="VGY24_001629"
     CDS             3400..>3825
                     /locus_tag="VGY24_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEQ4284590.1"
                     /translation="MNNRESKLTGFYDVFEEEVEKINHIHNVFVEESEKFGFQQMKTA
                     MVEHRKKYLDATDVHYTKIFEVHRVKEESVYALQSDLALSMSRCIANLPQQIPVKWIQ
                     EGVLFRDRIPTYKGYRRQFNQILLGAWGIESAYVDAEIIV"
BASE COUNT         1427 a          453 c          756 g         1189 t
ORIGIN      
        1 accacctttt acagttacga ataccatgtt aaataaggta gtagagattt ctaaaattat
       61 tggtaattta gaattacaag tacagaaaga tttaaaatta agaaaagaaa atcgtattca
      121 gtctattcac tcatcactcg ctattgaaca aaattcttta acagtagaac aaataacagc
      181 cattattgat gggaaaagag tgttagggaa cccccgtgag ataagagaag taaaaaacgc
      241 ttatgaagct tatgaagaaa ttttaacgct aactccctat gatgaatcac attttttaaa
      301 aatgaaggaa tttcaacagt acatttaccg gtaacaacat cgtcaatcac aagtcctatg
      361 gggttgggga gtgattcgct acctgtaaaa atttccttat ttgatgttga tacctatctt
      421 gcagattcga tgcagtttca cttagagtat atacttagac tttttccaaa gtcaggagtt
      481 cattacttaa tgcctacttt tagaggagaa gcagtagatc atcgacattt atcgcaattt
      541 tatcatagtg agtgtgagat agagggagat ttacacgatg tgatgaattt agtggataga
      601 tatattaagt ttctggttag tgaaattctt agagagtgtc gagatagtat ttttgctata
      661 acaaaagatg taagtcattt ggaaagtttt attgatcgaa agataccaat tattacgttt
      721 gaagaggctt gtactatatt ggaaaatgaa atagattcat tggaatataa tgatgaacat
      781 catttttatg tgttaacaga tgttggggaa aagaaattaa tggaacattt tgagggagta
      841 gtttgggtaa aaaattatcc ctgtaaaagt gtcccttttt atcaaaggtt ctcaacagat
      901 catctatttg cctataatgc agatttgttg ttaggaatag gcgaaactgt aggttgtgga
      961 gaaagatgcg aaacatatga agaagttgta gaaaatatga attatttaca agtagatgct
     1021 tctgaatata gttggtattt agaaatgaaa aaaacaagac ctatgaaatc ttcaggattt
     1081 ggtttaggag tagagcgttt aatagcgtgg atattgaagt gcgatgatgt tcgaaaagtt
     1141 caattaatca gtagagaaaa tgggattgat tatttaccat aaaggaggaa gaaaaatgaa
     1201 aaagaatatt ttaattgtag gtggaggtcc agagataact atatgttatg atttaattaa
     1261 caatagtacg gagtataatt tcttttcagt atcagaaatt cctacgatgt atccagagtt
     1321 gtttgtagaa acaattgaaa ccagctttag aaattttgga aagacgattg aacaaattaa
     1381 agagctcaaa tattctattg atgctgttgc ttgttgggat gaagctttaa cgcatatagc
     1441 tgatgatatt tcaaaagaat taggattgaa ccctatttca tcgctagatt ctcaatcatt
     1501 caggtttaaa gatcgaatga gaatggtatg tgaagctggt ggactaaaaa tgcccaaata
     1561 taaaattatc aatcaatttt cagatacaaa taaaataatc aattggaaat atccattaat
     1621 agtgaaacca acctcttttt tagcaagtat aggagtgaaa aaagtttata atttttcaga
     1681 attacaacaa gcagtttctc aaatgcttaa tgtaaaattt ccggtttaca tagcttcagg
     1741 ggtatatgaa ttgggggaat tatataattt agaacctaga gttttggtgg aagagtttat
     1801 tgatggagaa gaatatagct tagagagcgt ggtaagaaat ggaatctata cacctctagg
     1861 gataactaaa aaaatagtgg atgaaaaatt atttatggat gaaataggcc atattttccc
     1921 ttcaaattta aacaaagaag aaaaaagtag agtatattct tgggcagaaa aattacatca
     1981 aattttgcaa ttaaatcata ttacgactca tacagaattt agaattggaa gaaatggaga
     2041 tattatttta atagaaattg gagcaagaat cggtggagat tgtattccaa acttaatgaa
     2101 aaacagcttt atagtcaagg gatttgattt ttataatact tatttaaaag ctagactagg
     2161 tttacaagaa gagttaaata cagacactaa taagttttca ggaattgtat ttttcagact
     2221 caatccagag aattacggaa aaatatttaa tggagtgagt tacgatgcac ctaaaacttc
     2281 actaatattt gaagaaaaga tatataaaaa ggaaggaact gtattgccaa atccgattaa
     2341 ttggagtaat gagagagtag gatatgtctg tttggaaaac aaggaatacg ctgtgctaga
     2401 aaaggagatg aaagaaatat taaaatcatt gtcaatagag taaatagtga aagaaacatc
     2461 aaatctatag gtatttgttc accctcctat ccggggacat ttcattatca gaaacgatat
     2521 aagagaggag tagaattttt acaaaataaa ggatttacta tagtagaggg aaatttaaca
     2581 ggaaaagaag gttatagagg ggaaatttca ggaggagttc aagacagagc tgaagaatta
     2641 aatcaactta ttccgcgtgt ggatgtcatc atgacttcca taggtggcta caattctaat
     2701 tctgtattaa agtatataga ctacgatcta tttaaacaga aatctcctat ctttattgga
     2761 tattctgaca caacagctct tgctttagcc ttatataaaa agactggatg tataacttac
     2821 ctgtctcaga gcgttatctc taattttgga gagtttgaac cgtttaatga attaaattat
     2881 ttctattttg acttcatgct tcagagtaag tgcgaaacct taatggtaca aataccagat
     2941 gtttggacgg atgagtggat aaattgggaa acctatgaaa gaaccaaaaa aacaaataaa
     3001 aatgagtgga ttatttttaa caaaggtgaa tttaatggta ctttgatcgg aggaaatctc
     3061 gataccattg taggaataat aggaactgag tatatgccaa aaataacaga ggataccata
     3121 ctcttgttag aagatgtata tacggatcta ggaagactat ataggaattt cacaacgctt
     3181 gccttacatg gtatttttga taaaatagga ggacttatta tttcaaaatt tgaaacaata
     3241 ggggaaaata gcgatgttat taatgatatc attaatgaat ttgtagggca tagaaagatt
     3301 cctattttat tgaattttga ttgtggtcat actcatccta gctgtttaat gcctattggt
     3361 ggtaaaatta cgttgagttt aagctaaaag aggtggtata tgaacaatag agaaagtaaa
     3421 ttaacgggtt tctatgatgt ttttgaggaa gaagtagaaa aaataaatca tattcataat
     3481 gtttttgtag aggagtcaga aaaatttggc tttcaacaga tgaagactgc tatggtagag
     3541 catagaaaaa aatacttaga tgcaaccgat gttcattata caaaaatatt tgaagttcat
     3601 agagtaaaag aagagtcagt atacgccctc caatcagatt tggcacttag tatgtcgcgt
     3661 tgtatagcta acttaccaca acaaatacct gtaaagtgga ttcaagaggg agtgttattt
     3721 agagatagaa ttcctacata taaaggttat agaagacaat ttaatcaaat attattaggt
     3781 gcatggggaa tagaatcagc ttatgtggat gcagaaataa tagtt
//
LOCUS       DAUCWU010000114         3591 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUCWU010000114 DAUCWU010000000
VERSION     DAUCWU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.114"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(54..425)
                     /locus_tag="VGY24_001630"
     CDS             complement(54..425)
                     /locus_tag="VGY24_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ4284591.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            complement(492..1043)
                     /locus_tag="VGY24_001631"
     CDS             complement(492..1043)
                     /locus_tag="VGY24_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ4284592.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            complement(1306..2502)
                     /locus_tag="VGY24_001632"
     CDS             complement(1306..2502)
                     /locus_tag="VGY24_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ4284593.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(2695..3549)
                     /locus_tag="VGY24_001633"
     CDS             complement(2695..3549)
                     /locus_tag="VGY24_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ4284594.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
BASE COUNT         1134 a          724 c          554 g         1179 t
ORIGIN      
        1 ctactgtgat tgccattaaa taggtctcct ttaaatttta tacaattcat atcttaaata
       61 cgaaaattac caatatgctg tgagggagta gaaaaaccgc tttgtgtcag catttcatca
      121 tgtaagatcc gtctgacatc tgcatctgtt agagcttttt gtttttgcat taacttacct
      181 tgacgctcga catattcgcg tttaatagct gcaatattta atccctcaga caaaaaatct
      241 ttaatttcca aaagtcggtc catgtcattt aaggaaaaca tacgccgatt cccttgcgtt
      301 cgttcaggct taattaaacc ttgatcttca taatagcgaa tttgtctcgc agagagatct
      361 gttaatgtca tcaccgttcc aataggaaaa acagccattg agcgtctaag ttctttttct
      421 ttcatgacaa actcctttca tcttccttat tataggctga aaaaaataac ctgtcaagta
      481 tatatgttat attatctgac atcactgtgt tttttgttaa accatttatt tttaataagt
      541 tcaacatcag tattaactaa aatagctaca aagacgccac aaaatgtttc aaatacccgc
      601 gaagtcacat aaataaacgt ttgtcctgtt ggaatagaca gtgtaataat caaaagagca
      661 gccactgctc caataatacc tgatttatta ttacatgcaa cattaaccat gatacataac
      721 atggtacaaa taggaacaat taataaagta acccaaaaag cttgatgaaa tatctcattg
      781 aatgcaaaaa atactaagga gagtaggcca cctatactat taccaataat tctagaaaag
      841 ccaaagtgta cgcttttatc aaagtcttct cttaaactaa atacagcagt tagagcacca
      901 atttggagcc ctttccaacc aaataaatga aaaaccaata aaactaaaaa gaccgataag
      961 cctgttttca atgttctcat tcccaattta aactttttaa tatcaaactt gtaattagat
     1021 aataattttc ttaacgtttt catacctatc ccttcactat ctattttaac atatcaatcc
     1081 ataaaaccct tttggtaaac gttttattta tgattaggat atcttttata aaccaattga
     1141 tatgaaatcg tcataaactt ttgaaaattg cagtgtcagc caaataccac tatatctaaa
     1201 aactagtaaa tgataagtga agttagggaa agttcaataa ctgtacatga tcaactaaaa
     1261 aaagacatgc atcagcatgc cctttactat atattataat gactattatt tttcagtcaa
     1321 tgctgccaaa cctggtaata ctttaccctc gagaagttcc atgctagcgc ctccgccagt
     1381 actgatccat gagaatttgt cagcacggcc aaggttgata gcagcagctg ctgaatcacc
     1441 accaccgatg atggatttaa cgcctggttg tttaacgata gcgtccatga caccgattgt
     1501 accagcttgg aagtcagggt tttcaaagac acccattgga ccattccaaa caactgtttt
     1561 agcaccagta agtgcttggt caaactcagc gattgattta ggaccgatgt caagaccaag
     1621 gaagccttct gaaactgctt caccttcagt gtcgcgaact tcagtgtaac cagcaaatgc
     1681 gtttgcttct ttcgagtcaa ctggcaagat caatttacca tttgattttt caaggaggtc
     1741 tttagcaaca tccaatttgt cttcttctac aagtgagtta ccgatttcga taccttgagc
     1801 tttgtagaat gtgtaagtca taccaccacc gataagaact ttatcagctt tttcaagaag
     1861 attttcgata acaccaatct tatcagaaac ttttgagcca ccaagaatag ctacgaatgg
     1921 acgttctgga gtttcaactg cttcttggat gtaagcaatt tcgttttcaa gaaggaaacc
     1981 agctacagct ttttcaacgt ttgctgaaat acctacgttt gatgcgtgag cacggtgtgc
     2041 tgtaccaaat gcatcgttaa cgaagattcc atctccaagt gaagcccagt atttaccaag
     2101 ttcttcgtca ttcttagatt ctttcttacc gtcaacatct tcaaaacgag tgttttcaac
     2161 caaaagaact tgtccatctt ccaaagcatt gattgcttct tctaattttg aaccacgagt
     2221 aacacctggg aatacaacat cttgaccaag tttagcagct aaatcagcag ctaccggtgc
     2281 aagtgatttt ccttctttgt cagcttcttc tttaacacgt ccaaggtgag agaagaggat
     2341 agcacgacca ccttgttcga tgatatactt gattgttgga agagccgcag tgatacggtt
     2401 gtcgttagtg ataacgccgt ctttcaaagg cacattaaag tcaacacgaa cgaggacttt
     2461 tttacctttc aaatcaacgt ctttaacagt caatttagcc atgtgaatag actccttaat
     2521 attttttata ctcctccatt ataccacaat tcctagcata aagataaaat atgaaaataa
     2581 taaaataatc atttcacaaa atcaacattt ttttcagtca ttaaccctat tttcagaaaa
     2641 taatagtact taaaaagagg ttacattttc ttttgcacct ctttttcaat tactttattt
     2701 aaagcttttt aaacttttac ggcgttcctc tagttgctta agcacatcca gcttttcacc
     2761 tttataaatg gcaccttccc atgaaccata cataggatta gggaaaatga taaagcgtct
     2821 tccaaactct tcttgcaaat ctgataataa agctgttcta tcttcttgag atttttttga
     2881 aaaatcagca aaatctagaa gattatcacc aaatagcatc gttacattag ttgtttcttt
     2941 gaccttttga cgacgactct cctttgattt tacgcctttt tctaagaata gaagatgatc
     3001 acgaccttgt actggaatac cttctttttg gagattttcc attgtagcat ctacttgagt
     3061 agttgatctg tctgaaatgt agtaaatttg aacaccattt tggtctgcaa attgcaaaaa
     3121 gtctttagca ccagcaaccg gttttgcttc tttcttttgt acccaataat cccagctttc
     3181 aggtgtaaat cctgttcctt ccaaaacatt tttagcttga taagggctat tatcaagaac
     3241 tgtttcgtca atatctaata caattgaata aggtttatcc gttggtttat tgagttgttc
     3301 ttttaagcga tccgttgcta actgataccc ctgtaaataa agcgcttgag tttccgcagc
     3361 tcgctggtac cataaaacag acatggtatt ttcacgcgat cttaattggt catcactata
     3421 gctattatag gtttgtttag tagcaggctt acgattaact gatttgttgt tatcaacttt
     3481 agcacaacct gtcaccaaaa aaagggataa ggtaagtgat ataacactaa caactttttt
     3541 ggatttcatt gaaaatctcc tttttgctta ttaaattgat tttaaactta t
//
LOCUS       DAUCWU010000115         3486 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUCWU010000115 DAUCWU010000000
VERSION     DAUCWU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.115"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..580)
                     /locus_tag="VGY24_001634"
     CDS             complement(<1..580)
                     /locus_tag="VGY24_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4284595.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVY"
     gene            complement(926..3463)
                     /locus_tag="VGY24_001635"
     CDS             complement(926..3463)
                     /locus_tag="VGY24_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ4284596.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGFWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALARLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         1134 a          710 c          526 g         1116 t
ORIGIN      
        1 tgtaaacttc aacaaccgaa atcgtagtat cactatcgaa tccccataaa cgatcaaaaa
       61 tttgtgtctt tggtaaaata acgttttgat tttgcaaaag atacaccaac aaatcaaatt
      121 ctttgccaag taattcaact actttccctt caaccttcac ctcttttcga gctaagtcaa
      181 ccactaaatt gccaaaacta atatttttat ctgcaaattt ccctgttctt tttaacagtg
      241 cttgaattcg catttttaac tcttctaaat agaacggctt ggtcagataa tcatcagcac
      301 ctaattcaaa tccgtgacct ttatcatcta aaccctcttt agctgtcatg atcaacacag
      361 gaattttaat atctttttct cgcaattctt ttaagacttg aaagccattt ttctccggca
      421 acataaggtc taacaaaatc aaatcataaa taccactttc tgcttcgtat aaaccttcat
      481 cgccatcaaa aacttgcatg acgtctgcaa aatcatctaa aaaatcaaaa atggaatttg
      541 ataagctcaa gtcatcttct actaataata tttttatcat gtctctacct caattgttct
      601 taaataaagt aactatcact tcatttaact tagtctcatt tctgataaca atttctaacg
      661 agactatact acaaaaagct taaggtttac taaaatatta atactccttt aaggcattta
      721 aagcataatg attaaatcat ttataattgt tcttattcac cacctggtaa aaaagtattt
      781 aagaggctaa tagccttcgc tttaaagtgc aatctccttt tgaattaaac tatggataat
      841 catttagtta tagacggaac taagaaagga agctagctca ctacaaaaaa gagctggaaa
      901 gtcccccaac tcttttgctt atggtctagt aatgactgag agctgcaata actgcttctt
      961 tttctttagt gatcaaagca acacgtgctt caatttcatt aattcccatg gtaatattac
     1021 ggctaatagc catatcatct agcttaggtt caaagaaaga tttatattct gctagatgtt
     1081 ttggtgtttt aaagttactt gctggataaa taacaaattt atcaaagctc atatctccac
     1141 caagtgcaga tttaatccaa tcccaatttt ctctagccca ttcccaaatg atatcttgag
     1201 taaagctttg tccaagcaaa gcattgtacc aataagataa atcctgaggt ttaataatat
     1261 ctttatcttt taaactaaca agaatacgtc taatagtagt tggttgtttt gtttgactaa
     1321 aagcaaccgt taaatcactg cgtaaattat tatcagtagt tgctacataa gtctcaaaat
     1381 aaatatcaac caagctatct gtttcaaaat atttcatttg gttggctaaa accaagcgac
     1441 gaacagctgc tggaatactt gcaatattgt tttgatatgc ttcaaaaata gttctcaatc
     1501 cgtcaataat cgtctgattt tctgctagcc acaagcgggc caaggcaacc tgacgtacca
     1561 tctcatcttc atctgattcg tcagcttttt tctcaaaacc atgctgatta aagtcttctt
     1621 gatagatggt ggttaccaag cgattaaagg atttttctgc taagcttcct tcatcaatga
     1681 agcgtttcaa accagagacc acttgctgaa ccgcttctgc taccatataa gacttagaat
     1741 cgtccaattg agcaattaag tcaaccaatt cagcatagga aatcaagcca ctatctgcca
     1801 ataaacgacg ttcttgaata acttgtaaag cagaagtatt atcaagttgg gcaagttcag
     1861 tgactaaggc atctaataat gatccttggt agtctgtaat gtaatgagct gtattatcaa
     1921 tattgaaacg tagagctcct tcattttctg ctgccaattg actaaagttt ggaatgacca
     1981 tttctgcctc tgtcaaagtt tcaggaaggc catgccaatt agtgtttaaa ggaatcggcc
     2041 aaaggcgacc tttttcttca ccttttccaa taaaaaattg tttttgactc aaaatcaatt
     2101 gatcgttttc aaggcgagct gttaaaactg gataacctgg ttgttcaagc caagcatcca
     2161 taaaagcagc tacgtctttg cctgaagtat ctgataaaat attccataaa tcacgcccga
     2221 cagtatttcg atattgatat ttttcaaagt agtgatgtaa gccaatggca aaatcacgat
     2281 caccaataaa acggcgtaac atatgcatca aacgactacc ttttgcatag acaatagcag
     2341 gatcaaacaa tgtatttatc tcatctggat gattgacttc aacatgcact gattgaacac
     2401 catcagtagc atcacgtttt aaagcaagag gaataccgcc tgtttggaaa tcttctatta
     2461 ttttccaaga cggctcaata gcctcgatag agacatattc catcatgttg gcaaaactct
     2521 catttaacca cagatcgtcc caccatttca ttgtaaccaa gtttccaaac cattggtgtg
     2581 ctatctcgtg ggcgattaca agtgcaactt gttgtcggct ttgaacagtc gaattttcat
     2641 ctactaataa ataaatttca cgataagtga ttaaacccca attttccata gcaccagatg
     2701 aaaaatctgg aagcgcaata ttaagagatt gcgggattgg gtaatgtact ccaaaatagt
     2761 cttcataaaa gttgataaca cgaactgcaa tatccaatga aaaatcaagc gagctaagag
     2821 gatgcgctgt tgtcgcatac acaccaacag ttgttccttt tttgctttct acagtctttc
     2881 catgaagttc tcctagcgca aacgctagca agtaagatga cattcttggt gtcgtatcaa
     2941 atgtccaaaa accagtctcc tcgcgacggt caatattgac ttctggcata ttcgataacg
     3001 ctatctcacc aatttcttga tcaaaggtca agcttaaatc aaaagtagct tttgcttgtg
     3061 gttcatcgat acatgggaaa gcttcacgtg caaaatggct ttcaaactga gttgaaatga
     3121 cttctttttt ctcaccattt ttagtgtaat atgaagggta aatacctgtc atattatctg
     3181 taatgctacc tgaaaattca atgacaagcg tcatcattcc tgtttcaggt aattcaacac
     3241 gcacaacttc attgtcgtta tccacttgat aaataacagc ttcattatcg agcaaaatag
     3301 acttaatgtc caaatctttt tggtggaaag ccacatgatt atctaaagct tcaccattaa
     3361 tcgcaacatt ccccgtaaat gttttagtct gacgattaat atctaaaaag atgttataat
     3421 tttcaggcac aaatgtttcg atgagatgtt ctactgtctt catataactc cttaattcta
     3481 ataaca
//
LOCUS       DAUCWU010000116         3438 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUCWU010000116 DAUCWU010000000
VERSION     DAUCWU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.116"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..790
                     /gene="purR"
                     /locus_tag="VGY24_001636"
     CDS             <1..790
                     /gene="purR"
                     /locus_tag="VGY24_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ4284597.1"
                     /translation="NNPYKLTSLNTFATKYEAAKSSISEDIAIIKKAFEEANIGDIDT
                     LTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGYIYLSDLLSTPKILQNIG
                     RIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVRRDLKITEGSTVSVNYAS
                     ASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISLLTEFDSTLVGVAVFAEN
                     AQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKEREWT"
     gene            781..3402
                     /locus_tag="VGY24_001637"
     CDS             781..3402
                     /locus_tag="VGY24_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ4284598.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIEAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVSTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLEGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPSLTGVKLLSYSKVETTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
BASE COUNT         1201 a          656 c          693 g          888 t
ORIGIN      
        1 taacaatccg tataaattaa ctagtttaaa cacctttgca actaaatatg aggccgcaaa
       61 atcttctatc tcagaagata tcgctattat caagaaagct tttgaagaag ctaacattgg
      121 tgatatcgat acacttacag gagcaagtgg aggcgtaatt tttaccccca gtatttcaga
      181 aacagaagcg cgtaccatcg ttgaagacct gtgtcaaagg ttatcagaga gtgatcgcat
      241 cttacctgga ggctatatct atttgtcaga cttgctgagt acacctaaaa ttttacaaaa
      301 tattggtcgt attattgcca atgcctttaa aggagaaaag attgacgctg tgatgacagt
      361 tgcaaccaaa ggagttcctt tagcaaatgc agtggctaat attttaagtg ttccttttgt
      421 cattgttaga cgtgatttaa aaataacaga aggatcgaca gtgtctgtca attatgcgag
      481 tgcttctagt gatcgtattg aaaagatgtt tctgtcaaag cgtagtttga aacccaatag
      541 tcgtgtattg attgtagatg atttcttgaa gggcgggggg acgattacag gaatgatcag
      601 tctcttgaca gaatttgaca gcactttagt tggtgttgct gtgtttgcag aaaacgctca
      661 atcagagcgt gagcaaatga ccttcaaatc attgctgaaa gtttcagaga ttgatgtcaa
      721 aaacaacaat gttgttgtag aagttggtaa tattttcgat aaatggaaaa aggagagaga
      781 atggacttag aacaaacgaa gccaaaccaa gttaagcaga aaattgcttt aacctcaaca
      841 attgctttat tgagtgccag tgtaggcgta tctcaccaag tcaaagcaga tgatagagcc
      901 tcaggagaaa cgaaggcgag taatactcac gacgatagtt taccaaaacc agaaacaatt
      961 caagaggcaa aggcaactat tgaagcagtt gaaaaaactc tcagtcaaca aaaagcagaa
     1021 ctgacagagc ttgctaccgc tctgacaaaa actactgctg aaatcaacca cttaaaagag
     1081 cagcaagata atgaacaaaa agctttaacc tctgcacaag aaatttacac taatactctt
     1141 gcaagtagtg aggagacgct attagcccaa ggagccgaac atcaaagaga gttaacagct
     1201 actgaaacag agcttcataa tgctcaagca gatcaacatt caaaagagac tgcattgtca
     1261 gaacaaaaag ctagcatttc agcagaaact actcgagctc aagatttagt ggaacaagtc
     1321 aaaacgtctg aacaaaatat tgctaagctc aatgctatga ttagtaatcc tgatgctatc
     1381 actaaagcag ctcaaacggc taatgataat acaaaagcat taagctcaga attggagaag
     1441 gctaaagctg acttagaaaa tcaaaaagct aaagttaaaa agcaattgac tgaagagttg
     1501 gcagctcaga aagctgctct agcagaaaaa gaggcagaac ttagtcgtct taaatcctca
     1561 gctccgtcta ctcaagatag cattgtgggt aataatacca tgaaagcacc gcaaggctat
     1621 cctcttgaag aacttaaaaa attagaagct agtggttata ttggatcagc tagttacaat
     1681 aattattaca aagagcatgc agatcaaatt attgccaaag ctagtccagg taatcaatta
     1741 aatcaatacc aagatattcc agcagatcgt aatcgctttg ttgatcccga taatttgaca
     1801 ccagaagtgc aaaatgagct agcgcagttt gcagctcaca tgattaatag tgtaagaaga
     1861 caattaggtc taccaccagt tactgtcaca gcaggatcac aagaatttgc aagattactt
     1921 agtaccagct ataagaaaac tcatggtaat acaagaccat catttgtcta cggacagcca
     1981 ggggtatcag ggcattatgg tgttgggcct catgataaaa ctattattga agactctgcc
     2041 ggagcgtcag ggctcattcg aaatgatgat aacatgtacg agaatatcgg tgcttttaac
     2101 gatgtgcata ctgtgaatgg tattaaacgt ggtatttatg acagtatcaa gtatatgctc
     2161 tttacagatc atttacacgg aaatacatat ggccatgcta ttaacttttt acgtgtagat
     2221 aaacgtaacc ctaatgcgcc tgtttacctt ggattttcaa ccagcaatgt aggatctttg
     2281 aatgaacact ttgtaatgtt tccagagtct aacattgcta accatcaacg ctttaataag
     2341 acccctataa aagccgttgg aagtacaaaa gattatgccc aaagagtaag cactgtatct
     2401 gatactattg cagcgatcaa aggaaaagta agctcattag aaaatcgttt gtcggctatt
     2461 catcaagaag ctgatattat ggcagcccaa gctaaagtaa gtcaacttga aggtaaatta
     2521 gcaagcacac ttaagcagtc agacagctta aacctccaag tgagacaatt aaatgatact
     2581 aaaggttctt tgagaacaga attactagca gctaaagcaa aacaagcaca actcgaagct
     2641 actcgtgatc aatcattggc taagctagca tcgttgaaag ccgcactgca ccagacagaa
     2701 gccttagcag agcaagccgc agccagagtg acagcactgg tggctaaaaa agctcatttg
     2761 caatacttaa gggactttaa attgaatcct aaccgccttc aagtgatacg tgagcgcatt
     2821 gataatacta agcaagattt ggctaaaact acctcatctt tgttaaatgc acaagaagct
     2881 ttagcagcct tacaagctaa acaaagcagt ctagaagcta ctattgctac cacagaacac
     2941 cagttgactt tgcttaaaac cttagctaat gaaaaggaat atcgccactt agacgaagat
     3001 atagctactg tgcctgattt gcaagtagct ccatctctta cgggcgtgaa actgctatca
     3061 tatagtaagg tagagactac tccgcttgtt caagaaatgg ttaaagaaac gaaacaacta
     3121 ttagaagctt cagcaagatt agctgctgaa aatacaagtc ttgtagcaga agcgcttgtt
     3181 ggccaaacct ctgaaatggt agcaagtaat gccattgtgt ctaaaatcac atcttcgatt
     3241 actcagccct catctaagac atcttatggc tcaggatctt ctacaacgag caatctcatt
     3301 tctgatgttg atgaaagtac tcaaagagct cttaaagcag gagtcgtcat gttggcagct
     3361 gtcggcctca caggatttag gttccgtaag gaatctaggt gatgcattcg tcataagcaa
     3421 ttgcatcaaa gaaacacg
//
LOCUS       DAUCWU010000117         3306 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUCWU010000117 DAUCWU010000000
VERSION     DAUCWU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.117"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..800
                     /locus_tag="VGY24_001638"
     CDS             <1..800
                     /locus_tag="VGY24_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase"
                     /protein_id="HEQ4284599.1"
                     /translation="KQKASEEEIKKLKGELDKKIEEAKKLIEEANKKSKEELEKQAKD
                     EKEKNQNQDLSKKLDELLKLQKENREKKEDKKSQDKKWDELLKTDDKNILKQFDLNKM
                     KKQEEQQDKKQAKDEKEFAVFQVDKNFYNIINKDGKTTVYMDVKTYVDQGRTMIPVRY
                     IAYTLGFNVEYDNSTREAIFSNKENNILAKKTLRLNIDTGVMKDSDGKVYNSDVKPVI
                     INGRIHASISNIAKAFGASHGDIKDGKNQTIEWDNARKAVYVFKNVK"
     gene            815..1675
                     /locus_tag="VGY24_001639"
     CDS             815..1675
                     /locus_tag="VGY24_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEQ4284600.1"
                     /translation="MNKSIKRGVAIALLLFTFTVPATTFAMTNEGQIESQNKSIYEPQ
                     KEEFEKMLKDDVFTPSKEEIPYQDVPRIPGNTGEANNPSNNPPKKTPLVKGGNTKAVN
                     SLATQENKARGSVIENVDRNGKDITPSGDTEKDKENPVDVRQFLTFQTKSGKTMHLIV
                     DHSSNQDNVRLLTEVGEQDLLNMIESEDKNTIKVEEPKKEEVKKEEPKTVPVKEEKKS
                     GIGSFLIIALVIGGVIGAGYYFKVVKAKEDRMLEDFEEDDEDYISESEDESDNEESHE
                     ESLDEDDELL"
     gene            1749..2360
                     /locus_tag="VGY24_001640"
     CDS             1749..2360
                     /locus_tag="VGY24_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284601.1"
                     /translation="MSSYTEKISDKINDFDSHKVFFANDFLDIASYETARKTLNRMVN
                     ERKIKRVIYGFYYNPRYSELIGEYEAVSIHELALAIARKYNWNIAPYSSTALNLLGLS
                     TQVPTHYKYISSGRYKEYKIGDTVLEFKKVNPGEIANMSLKTATVIQAIKSLGKENIT
                     NEVIQKIRENLSEKERIDLMNESKSVPAWIYEVIREICEGKNE"
     gene            2353..>3306
                     /locus_tag="VGY24_001641"
     CDS             2353..>3306
                     /locus_tag="VGY24_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEQ4284602.1"
                     /translation="MSKFYIENKEDLRVLIVNTARKKNISEAVIEKDYWVTFILDYLF
                     NENKWKDCFTFKGGTSLSKCFGLIERFSEDIDLILDWRVLGYEEKEPWLERSNTKQDK
                     FNKAVNEKTEVFLRDEFLKVLEQDLKDFDFEFSIDSIDSQTILCKYPKIFESNYLTQN
                     IRLEIGSLAAWTPAIDVEVLPIISEVYPNIFKEKTNIRTVSAERTFWEKTTILHHEAN
                     RPESSPMPHRYARHFYDLYKIANSDFKNKALEDKELLKKVTEFKMKFYPRKWARYEDA
                     LIGKLKLVPREFRFSEIEKDYKAMEEMIYGDYPNFEEIIKVL"
BASE COUNT         1442 a          391 c          604 g          869 t
ORIGIN      
        1 aaaaacaaaa agcttctgaa gaagaaatta aaaagctaaa aggagaatta gataagaaaa
       61 ttgaagaagc aaagaagtta atcgaggaag caaataaaaa atcgaaagaa gaacttgaaa
      121 agcaagctaa agatgaaaaa gaaaaaaatc aaaaccaaga cctatctaaa aaattagatg
      181 aacttctaaa actccaaaaa gaaaacagag agaagaaaga agataagaaa tctcaagata
      241 aaaaatggga cgaacttttg aaaacggatg acaagaacat cttaaaacaa tttgatctta
      301 acaagatgaa aaaacaagag gaacaacagg acaaaaaaca agctaaagat gaaaaagaat
      361 ttgcagtttt ccaagtagac aaaaactttt ataacattat caacaaagat ggtaagacaa
      421 cagtctatat ggatgtaaaa acttatgtgg atcaaggaag aactatgatt ccagtaagat
      481 atattgctta tactctaggt tttaatgttg agtatgacaa ctctactcgt gaagccattt
      541 tctcaaacaa agagaataat atcttagcta aaaagacatt aagactaaat attgatactg
      601 gtgttatgaa agattcagat ggaaaggtct acaattcaga tgttaagcca gtgattataa
      661 atggaagaat tcatgcttca atttcaaata ttgctaaagc ttttggagca agtcatggag
      721 atattaaaga tggcaaaaac caaacaatag aatgggacaa tgctagaaaa gcagtctatg
      781 tatttaaaaa tgtaaaataa gaaaggatag aaaaatgaat aagagtatta aacgaggagt
      841 agccatagcg ttactccttt ttacatttac agtaccagca actacatttg cgatgacaaa
      901 tgaaggacag attgaaagtc aaaacaaatc aatctatgaa ccacaaaaag aagaatttga
      961 gaaaatgttg aaagatgatg tttttacacc atcaaaagaa gaaattcctt atcaagatgt
     1021 tccaagaata ccaggaaata caggtgaagc aaataatcct tcaaataatc ctccaaagaa
     1081 aaccccactt gtaaaaggtg gtaatacaaa ggcagtaaat agtctagcaa cacaggaaaa
     1141 taaggcaaga ggttcagtaa ttgaaaatgt agataggaat ggaaaagata ttacaccaag
     1201 tggagataca gaaaaagata aagaaaatcc agttgatgta agacaattct taacatttca
     1261 aaccaaaagt ggaaaaacta tgcacctaat agtggatcac tcatcaaatc aagataatgt
     1321 aagattatta acagaagtag gagagcaaga cctacttaat atgattgaat cagaagataa
     1381 aaatactata aaggttgaag agcctaagaa agaagaagta aaaaaagaag aacctaagac
     1441 tgttccagta aaagaagaaa agaaaagtgg aataggttca tttctaatta ttgcacttgt
     1501 aattggagga gttataggag caggatacta ttttaaggta gtcaaagcca aggaagatag
     1561 aatgttggaa gactttgaag aagatgatga ggattatatt agtgagtcag aagatgaatc
     1621 tgacaatgaa gaaagtcatg aagaatcttt agatgaagat gatgaactat tataaggata
     1681 ttaaatttaa gtacaattga acttttgtcc cagatatgat atgatatttg ggacaaagga
     1741 ggattgtaat gagttcatat acagaaaaaa tatcagataa aataaatgat tttgactcac
     1801 ataaagtatt ttttgctaat gactttttag atattgcttc ttatgaaact gctagaaaga
     1861 cattgaatag aatggttaat gaaagaaaaa ttaagcgtgt catttatgga ttttattaca
     1921 atccaagata tagtgaatta attggagagt acgaagcagt atcgattcac gagttagcac
     1981 ttgccatagc aagaaaatac aattggaata ttgcaccata tagtagcaca gccttaaact
     2041 tattaggact ttcaacacaa gttccaactc actataaata tatatctagt ggaagatata
     2101 aagaatataa aattggggat acagttttag aatttaaaaa agtaaatcca ggagaaattg
     2161 ccaatatgtc tttgaagaca gcgacagtta ttcaagcaat taagtctttg ggaaaagaaa
     2221 atataactaa tgaagttata caaaagataa gagaaaattt aagtgaaaaa gaaagaatag
     2281 acttaatgaa cgagtcaaaa tcagtgccag catggatata tgaagtaata agggaaattt
     2341 gtgagggcaa aaatgagtaa gttttatata gaaaacaaag aagatttaag ggtcttaata
     2401 gtcaatactg caagaaagaa aaatatatct gaagcagtca ttgaaaaaga ctattgggtt
     2461 acttttatct tagactatct atttaatgaa aataaatgga aagattgttt tacctttaaa
     2521 ggtggcacat cactttcaaa gtgttttgga cttatagaaa gattttctga agatatagat
     2581 ctgatcttag attggagagt attaggctat gaagaaaaag aaccatggtt agaaaggtca
     2641 aataccaagc aagataaatt caacaaagca gttaatgaaa agacagaagt atttttaaga
     2701 gatgaatttt taaaagtttt agaacaagat ttaaaggatt tcgattttga attttctata
     2761 gatagtatag attcacaaac aattctatgt aaatatccta aaatttttga atctaattat
     2821 ttaacacaaa atataaggct tgaaatagga agccttgcag catggacacc tgcaattgat
     2881 gttgaagttt tgcctataat tagcgaagtc tacccaaata tatttaaaga aaaaactaat
     2941 ataagaacag tatcagcaga gagaactttt tgggaaaaga caactatact tcatcatgaa
     3001 gcgaatagac cagaatcttc tcctatgccc cacagatatg caagacactt ttatgactta
     3061 tataaaatag caaattcaga ttttaaaaac aaagccttag aggataaaga attactaaag
     3121 aaagtgactg aatttaaaat gaaattctac cctagaaagt gggctaggta tgaagatgca
     3181 ttaattggaa aattgaagtt agttccaaga gagtttcgat tttctgaaat agaaaaagat
     3241 tataaggcta tggaagaaat gatttatgga gactatccaa actttgaaga gattataaaa
     3301 gttctt
//
LOCUS       DAUCWU010000118         3268 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUCWU010000118 DAUCWU010000000
VERSION     DAUCWU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.118"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(94..603)
                     /locus_tag="VGY24_001642"
     CDS             complement(94..603)
                     /locus_tag="VGY24_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284603.1"
                     /translation="MELFNTIIGVIALIVAVIALVHSIYYNMVKIKLSDCYISRVDKG
                     YDWMYDFSISNLSNVSVIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLS
                     MPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQL
                     FFPHFDNNS"
     gene            complement(862..1071)
                     /locus_tag="VGY24_001643"
     CDS             complement(862..1071)
                     /locus_tag="VGY24_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284604.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(1247..1465)
                     /locus_tag="VGY24_001644"
     CDS             complement(1247..1465)
                     /locus_tag="VGY24_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015990842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284605.1"
                     /translation="MSKLSPKPTRRTKFKTWKDLDRTLKNSFGECNFDSATIILDEYE
                     ISKEEIILEATQQGYKVIDNNDNYLTFE"
     gene            1608..1784
                     /locus_tag="VGY24_001645"
     CDS             1608..1784
                     /locus_tag="VGY24_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284606.1"
                     /translation="MKKKLDKILIDKGMSKKELSEKTGISYNTIMNIGKKDISFNKMK
                     KIADVLGVSLDEFR"
     gene            1862..2158
                     /locus_tag="VGY24_001646"
     CDS             1862..2158
                     /locus_tag="VGY24_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284607.1"
                     /translation="MAIEIFGPEFRKKLLEDLIALNMEAIKIAQTKNAKSIEWITMKR
                     LEKETGWGRTKLTQWREQGKFNFKRSSENGKVLYDLADVNRFLRTSGYEKGETT"
     gene            2155..2292
                     /locus_tag="VGY24_001647"
     CDS             2155..2292
                     /locus_tag="VGY24_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284608.1"
                     /translation="MKLLDFIFAKPKKQELIKHDTLRASSEKQWAEFDAYMRKQYGRQ
                     A"
     gene            2373..2702
                     /locus_tag="VGY24_001648"
     CDS             2373..2702
                     /locus_tag="VGY24_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284609.1"
                     /translation="MKTKIEIMRDFFKENPESTQRQASEKLGFTENTVKQYIWRDVKR
                     GYCIKDEEGRVTYLNIEDELSLINEWKSEIRRELIEQLLSANRHETSSEQIRMNAKTI
                     NQILGEI"
     gene            2702..2896
                     /locus_tag="VGY24_001649"
     CDS             2702..2896
                     /locus_tag="VGY24_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284610.1"
                     /translation="MEGMKKYINGQIKLIDTIEECVKEKWPEFDDKKVHKISLAIYQG
                     WSLTDINSTLEAIEMDLDRK"
     gene            2893..3177
                     /locus_tag="VGY24_001650"
     CDS             2893..3177
                     /locus_tag="VGY24_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284611.1"
                     /translation="MRIYVNKKGKPSVEFEFEDRRGGMFDTRLMLKESPLKEEFKADM
                     YKAIDGVLKKYEDIFDTPIFKELIIGKEEDIRKMIDYDDKFTELFGGIRH"
BASE COUNT         1271 a          468 c          592 g          937 t
ORIGIN      
        1 gcacgcagga ggaacaaaac taataaaagg agaaaagaaa aaatgctact aacggcagca
       61 cttatcttaa ttttattaac gaatatcgct atcttaacta ttattgtcaa aatggggaaa
      121 gaataactga tattttcgga attgatgaat acgcttatca gttgtgattt tgattttaac
      181 tggcttttca tctaaatagt aagagaaatt ttctcgacta gatggatata cttcagtatc
      241 tgacttaaat ggcgatgact cccattggta atctaatggc atataataat tcggcattga
      301 caatccataa taatcatcag cttcattctg catgtcagct tcgtattttt gaaagggatt
      361 gaagccatta tcgcttatta gttttccatc tttgttgtaa agttcaattt ttttaataat
      421 gactgaaaca ttcgataagt tactgatact aaaatcatac atccaatcgt aacctttatc
      481 tactcttgaa atgtaacaat cagataattt tatcttaacc atgttgtagt agatagagtg
      541 aacaagagca atcacagcaa caattagcgc aatgacgccg ataattgtat taaataattc
      601 catttttatt cttccaatcg tttttatttt aattatacca aaatagaaag gacacatatg
      661 aacgaaatag aaaacaaact agaagaactt gaagaaatgg taataaacat ggatgaagta
      721 gatgtagtga ttccatggaa gatagcaaaa aacctgctac aaagagcagg ttacttatca
      781 gaagaggaac atcgcctact aagttggagg ctcggaaaat caaaatatgc aggcaaacgt
      841 tccgaaaaag tccgcaattt gctagaggga cttaggaata gtggaagttc cgagaatagc
      901 tgataatttt aattctgttg tttggtcatt gtgcatgaaa atgagagttt catttttgcg
      961 attaggctca atccaatgat caataacaaa gtctccgaca agactatgta aaataacagt
     1021 ttcaaaatca tcaatactat tcaatagagg agttaattct tctaaattca tttagttacc
     1081 ctccttccta ataggataac tacattatac aacagaaagg aaaccacatg agaccaaaac
     1141 gatatccgta tagcggagag aaaaagcaac ctgataaaca gattgccgag ttgcaatata
     1201 aggttatgaa taattcgagt aatttggcaa aattaacaac catactttac tcgaaagtca
     1261 aataattgtc attgttatct atgactttat aaccctgttg tgttgcttct aagataattt
     1321 cttcttttga aatttcatat tcatctaaaa taattgtcgc tgaatcaaaa ttacattcac
     1381 caaaactgtt ttttaaggtg cggtctaagt ctttccatgt tttgaatttt gttcttcttg
     1441 taggttttgg actcaattta ctcatgcaat atcctccttt ccataatttt ggaatacaac
     1501 ggtgagaggt catattcaaa taaattatat cagaaaggga ctaaaaacac aatatattgt
     1561 gttatcgata tataaaaaaa cacaacatat tgaagtttgg ataatctatg aagaaaaaac
     1621 tagacaaaat tctcattgat aaaggaatga gtaaaaaaga gttatcggag aaaacaggaa
     1681 ttagttacaa caccattatg aatatcggta aaaaagatat ttcgtttaac aaaatgaaaa
     1741 aaattgccga tgtcttgggc gtcagcttag acgaatttag atagtaaaaa agtccgacgg
     1801 gaaacggact caaaacaaat tttaatttac ttaattataa cataagagag agggaaaaac
     1861 tatggccatt gaaatatttg gacctgagtt tagaaaaaaa ctgcttgaag atttaatcgc
     1921 tctaaatatg gaagcgataa aaatagcgca gaccaaaaac gccaagtcta ttgaatggat
     1981 aaccatgaag cggctagaaa aagaaactgg atgggggcga actaaattga cccagtggag
     2041 agaacaaggg aaatttaact ttaaaaggtc atcagaaaac gggaaagtac tatatgacct
     2101 agcagatgtt aatagatttt tacggaccag tggatatgaa aaaggagaaa caacatgaaa
     2161 ttattagatt ttatttttgc aaaaccaaaa aaacaggaac tgattaagca cgacacgctt
     2221 agagcatcat ctgaaaaaca gtgggccgaa tttgatgcct atatgaggaa acagtatgga
     2281 cgacaagctt aatcagatat tgatagccat gcgaaactac cacacaaatg gtgatgatag
     2341 caagtattta gaggatatgg aggtcatttt aaatgaaaac aaaaattgaa atcatgcgtg
     2401 atttttttaa agaaaatcct gaatcaactc aacggcaagc ctcagaaaag cttggattta
     2461 cggaaaatac agttaaacaa tatatttgga gagatgttaa gcgtggctac tgtattaaag
     2521 acgaagaagg tcgtgtaact tatctaaaca ttgaagatga attatcgctt attaatgaat
     2581 ggaaaagtga aattagaaga gaattgattg aacagttact atcagctaat cggcatgaaa
     2641 cgtcaagcga acaaattcgt atgaacgcta aaacaatcaa tcagatttta ggagaaattt
     2701 aatggaaggt atgaaaaagt atatcaatgg tcagattaaa ctaatagata ctattgaaga
     2761 atgtgtcaaa gaaaaatggc ctgaatttga tgataaaaaa gtacataaga tatctttagc
     2821 tatttaccaa ggatggtccc ttacagatat taatagcaca ctcgaagcga tagaaatgga
     2881 cttggataga aaatgagaat ttatgtaaac aaaaaaggta aaccatctgt tgaatttgag
     2941 tttgaagatc gacgcggtgg catgtttgat acaagattaa tgttaaaaga atcaccactc
     3001 aaagaagaat ttaaagccga tatgtacaag gcaattgatg gtgttttaaa aaaatacgaa
     3061 gacatttttg atactcctat ttttaaagaa ttaataattg ggaaagaaga agatattaga
     3121 aaaatgattg attacgatga taaatttacc gaactttttg gagggattag acattgaaga
     3181 ttactaaagc gacagaaatt acaaaaaccc ataattggcg catattaatc tatggtaaac
     3241 ccggaaacgg gaaaacttac ttaactaa
//
LOCUS       DAUCWU010000119         3216 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUCWU010000119 DAUCWU010000000
VERSION     DAUCWU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.119"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..663)
                     /locus_tag="VGY24_001651"
     CDS             complement(<1..663)
                     /locus_tag="VGY24_001651"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ4284612.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKYITYKELADQQILNDIEIIINTTPVGMINYEE
                     SLP"
     gene            complement(732..803)
                     /gene="shp3"
                     /locus_tag="VGY24_001652"
     CDS             complement(732..803)
                     /gene="shp3"
                     /locus_tag="VGY24_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ4284613.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            883..1734
                     /gene="rgg3"
                     /locus_tag="VGY24_001653"
     CDS             883..1734
                     /gene="rgg3"
                     /locus_tag="VGY24_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ4284614.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     SLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1987..>3216)
                     /locus_tag="VGY24_001654"
     CDS             complement(1987..>3216)
                     /locus_tag="VGY24_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ4284615.1"
                     /translation="EALTAIAKKKNALTCDIDDIKENKDLIRQKTQNIHQALSQARLQ
                     ERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQDSIQRLPQWQKQLQDA
                     TEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTFIRRQTKLETHLEQVAN
                     RLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQKTIRALGPINSDAINQY
                     EEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVTFEAIRKSFKETFTQMF
                     GGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKALSALALLFAIIRVKTI
                     PFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGTMAAADSIYGITMQESG
                     VSKIVSVKLKEAQEMTN"
BASE COUNT         1016 a          591 c          467 g         1142 t
ORIGIN      
        1 aggtaaactt tcttcataat ttatcatacc aactggtgta gtatttatta tgatttctat
       61 atcatttaaa atttgttgat cagccaattc cttatatgta atatacttta aatcagggaa
      121 actctttatc aaacttcttg tattctcact aattggctcc ctgtatacaa ctgttggtga
      181 gatttgtagt ttttgtaaag tgaaaatagc agctctagca gtaccgccag tacctaaaac
      241 acaacatttt gttatatggc taatattaaa gaaaactaat gtattataaa tacctttcca
      301 atcagtatta gttcctgtaa ttatatttcc agatttataa atattgttaa cagatccagt
      361 tatctctgct actctgtcta aattgtcaat atacctcatt acctctcttt tataaggtaa
      421 agttatattt aatccaatac attttggatc atttatcaga tgtttgattt tatcttctaa
      481 acacactgag tctaccaaaa tttttttatg tttatattca tccagaactc ctatgttgtg
      541 atttagatat tcaaataatt catctgatat agaatgttca actggattac caagcaatgc
      601 agaatacata aatttcatcc tcctacgttt aattagttta ctactaacat aaagtacaag
      661 cattacacag agctaaccat tacctataag tctttagata ctctaaagac ttaggtgtac
      721 acctaagtaa cttacccacc aacaataatg ataatatcca ttgctaaaat taaaatcggc
      781 aaaaattttg aaattttctt cataagtgga cttcctttca gtttttattt attgtacaat
      841 aaaaaaaaac tgaattgatt tttgttggga aagtgggaaa atatgaaatc aaaacttggt
      901 gccacactta gaaaagttcg aaaaggaaag caaattagtc tgtgcttcgt ggcagatgaa
      961 cacctttcaa aatcacaaat ctctcgtttt gagcgcgggg agtcagagat atcgtgtatc
     1021 cgactgatca atattctaga caaattacac attactttgg atgagtttct tattttacat
     1081 gatgaagact ataccaattc cgaaacattt gctaacctgg tacagtatat tcgtaagcaa
     1141 tattcatcac aaaatatcaa taatatagca tgtttacttt ccgacacttc tgattacact
     1201 ctgaattctt ttgagaaaac aatggtaaag tccattttac acacaatgga ttcaagtatc
     1261 attccatcag atgatgaatt acttcaacta acagattatc tctttaaagt tgaaaaatgg
     1321 ggctactatg aaatcctttt gttgggtaac tgtgtacgaa caattaacta caactcctac
     1381 ttcctattga caaaggagat gttaaataat tacatttact cttcacggaa caaaactaat
     1441 aaacgaattg tcactcagct tgctataaat tgtttcattc ttagtatcaa taaggaggaa
     1501 ttttctaatt gctcctattt aattgatgag ataaaatcat tattagataa tgaattaaac
     1561 ttctatgaac agacagtttt tctctatgca acaggttact ttgaatttaa gcgccaatta
     1621 gaaactggag ttgaaaagat gaaacaagcc atacaagttc ttgatatttt aaatgaagac
     1681 aagttaaaat tacattacgc tgaccatttt gataaactag tcaacaaaga ttaagtgaaa
     1741 tcattcttta ccttttaacg aaatgccaat caagagtgtc tcaataaaaa atcatctact
     1801 ctgacaactt ttctctttta cctatcgaca gctaaaaagc cttggaaact gctatttcca
     1861 aggctttctt tgtactctat tgtatcaatt agcttattcc gatttctgtt gctattagtt
     1921 taatcatgaa atctcctaac ttatgaccga acatccaaaa aaacagaagg aactcccttc
     1981 tgttttttaa ttggtcatct cttgagcttc ttttaatttt acagatacaa ttttagagac
     2041 ccccgattcc tgcatggtaa ttccataaat actatctgct gcagccattg ttcctttacg
     2101 atgggtcacg acaataaatt gactatcttt gtcaaagcga ttgaggaaat ccccaaagcg
     2161 cttaacattt gcttcatcaa gagccgcttc tacctcatct aaaatcacaa acggaatcgt
     2221 tttaacacgt ataatagcaa acaataaagc cagtgctgac aaagctttct ctccgcctga
     2281 catcaagtta agagattgaa ttttcttacc aggaggctgc acagaaatct ctataccagc
     2341 agacaagaga tctgtttccg ttaaaaccaa atcagcagaa cccccaccaa acatctgagt
     2401 aaaggtttcc ttaaaacttt ttcgaattgc ctcaaaagtc actttgaaac gagccttcac
     2461 ttcgctatcc atactattaa tagtctctag caaaagattt ttagcttttg tcagatctgt
     2521 cttttgactt gttaaaaaag tcaaacgctc atgcacttcc tcatactgat taatagcatc
     2581 actattaatt ggtcctagtg cacgaatagt cttttgtaaa tggtgtaatt tttctttggc
     2641 actttctaag tgatcaatac tatttgttac ctcctttgcg tctgctagtg tcatttgaaa
     2701 atcttctgat aaacttttag cataggctcg taagcgatta gccacttgct ctaaatgtgt
     2761 ctctaacttg gtttgacgac gaataaatgt gtcatttttt tcgctttctt tggttatttt
     2821 ttctgctgtt tcttctaagc gggcctcata gtcctctatt tcaaatctta attggactaa
     2881 acgtttttga gcaccagact tatgctctgt ggcatcttgt aactgttttt gccactgggg
     2941 tagtctttga atgctgtctt gacttacgtt attgtttagt atgctttcaa gtttgagaat
     3001 attttgctgg cattgcttca gctgagttct tagacgagat tgattagctt gctcaaattt
     3061 cttttcatta agtaaatctc tttcttgtaa acgagcttga gataaagctt ggtgaatgtt
     3121 ttgtgttttt tgcctaatca aatctttgtt ttctttgata tcatcaatat cacaagttaa
     3181 ggcatttttt ttcttagcaa tagctgttaa tgcttc
//
LOCUS       DAUCWU010000120         3080 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUCWU010000120 DAUCWU010000000
VERSION     DAUCWU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.120"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(57..1010)
                     /locus_tag="VGY24_001655"
     CDS             complement(57..1010)
                     /locus_tag="VGY24_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4284616.1"
                     /translation="MTFEKIEEAYQLLLENCQLIKNDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMESFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            complement(1108..1434)
                     /locus_tag="VGY24_001656"
     CDS             complement(1108..1434)
                     /locus_tag="VGY24_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ4284617.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            complement(1418..1852)
                     /locus_tag="VGY24_001657"
     CDS             complement(1418..1852)
                     /locus_tag="VGY24_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ4284618.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            complement(1845..2081)
                     /locus_tag="VGY24_001658"
     CDS             complement(1845..2081)
                     /locus_tag="VGY24_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ4284619.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            complement(2086..2514)
                     /locus_tag="VGY24_001659"
     CDS             complement(2086..2514)
                     /locus_tag="VGY24_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ4284620.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            complement(2489..2815)
                     /locus_tag="VGY24_001660"
     CDS             complement(2489..2815)
                     /locus_tag="VGY24_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ4284621.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            complement(2817..>3080)
                     /locus_tag="VGY24_001661"
     CDS             complement(2817..>3080)
                     /locus_tag="VGY24_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ4284622.1"
                     /translation="QAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQ
                     FFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
BASE COUNT          939 a          582 c          516 g         1043 t
ORIGIN      
        1 ttaacctctc ttgaaatcgt tttctgtgat gcttttttat tataacaaaa aatcaattaa
       61 ttgacattac tcagtttcca ttttttgaaa ttttccataa aatcatgaat attctctgca
      121 agcttcaaat cccgaattgg atagacaaag ctttccattg ggtggtctgt ttgtttttgt
      181 aagacaataa tggacttggc atttgaggga tgaccaaaaa tagaatctgg tagcgtaatc
      241 aaggccacca cctgagcata atcttttaac cactgtttca ataactggct ctgagggctc
      301 gtcaataaat tgactggtgc cagaaaaatc gcaaaaccgt cttttttcaa gtattttaaa
      361 gactgttcca ttaataaatg atgggcatag gtatgcttat ctgaactagc caccttgtac
      421 cgtttggcaa tatcatcgtt aggataataa ccaactggta agtcactgat gacaatgtca
      481 ctttctttta gtaattgagg ccttaccgca tcttcttgaa taaaatgagc tgaagaatcc
      541 attatctcag caatactggc tgacagatca atcaagagat catcaagttc aatccctaca
      601 taatcaaggc tcttgctcgt gttgttgaga agggtttggg ctaggttccc tgttccactt
      661 ccaatctcaa gtacctctaa gctatcttta tcactcaatt gttccaaaag atacagcaga
      721 atgaagccaa tagcatctgg tgtgaactga tggttggctt ggagttgctc cgtctgagct
      781 gccttaataa aaagaaactg gtaggcctta cgccattctt cttttgtcag gcacaaggct
      841 ttcagtttat cactattttg agcaacttga ggactagctc cctcagcccc taaataaaag
      901 gagttttgtt caacaatggc atcgtatata tgtgttttta gatcattttt aattagttga
      961 cagttctcta agagcagctg ataagcttct tcaatttttt caaaagtcat aaccttatca
     1021 taccaaatta cacttgaaat gaaaagaagc gtcaccttta attactatat attggatcat
     1081 tttaaggatt tatgacaaca atactattta ttgactatat tgtttgggtt taaggtattc
     1141 aaactgatac tgtcgttgct gatctaactt aacaattaac atccatctct cttttcgctc
     1201 tcgacaaatg acttgaccac ctttaaaaaa gacaggatta gaagctttat atctatgatc
     1261 ttgataaact atttctgcta tcagtcgggc tttaacatag tcagtttgag cttggtattc
     1321 cttgtgtaga gctgtcactt gagaaaggta aacctgtaaa accagcaaaa aaatactagc
     1381 cataaacata gcatacaaaa gaatgcctgc tttgagatta gtcttgaaag gcataataaa
     1441 aagtcctttc taacccactt ttccacttta aagtaatgca aatctgtgac tgctctatgt
     1501 gaatatgact acgtgatatt ccaaataaca tgggttgata cccttttcca ttactagctg
     1561 attttcgaaa atcatgactt ttaaattggc caaaagctag tactttcttt cctttttcaa
     1621 cgtatagttt attatcagct actttgtaaa atcttgctcc acttaactcc tctcgcaatt
     1681 gatgagaaaa taagagccaa ttgtcttgat catgacgggc tagataatgg ctacgtttaa
     1741 ggagggtttg ggtcaaacct tgataaacca ataaagaccc tgatatcacg agtaaggcta
     1801 ttaacgcctc tagaagggta aaagctttta tgttacttaa ttgtttactc aatcttcatc
     1861 acttctcctt cgtcatcgtg aatactaatg tatctatccg tacgtaaaat agtaatatga
     1921 caaccattca atgttatctc tttagttcta gtttgtactg ccatcaaggc tagattaagc
     1981 ttttcttgct gctttcgata gtacgttaga gcagcctgac tctgctgtaa actgcttaag
     2041 acgagaatga caatactaaa gaggatgcct gttgcaatca tactttcaag ggctatgtaa
     2101 gctttgactt gttttttgat aattcccact ccctagataa aattggtatt taaagcgtcg
     2161 atcagtcatg tcaaaaataa ttttggctaa ggaatggttg cctcccatct gatcaataac
     2221 taaccgataa ggatgattca ctgttattga tttaggaact gttaggctat taccttctgt
     2281 aacaattttt gttgaagaaa tatccaagac gcgctgtttt tgctgtaata tggctaactt
     2341 ttgctgatgt cgatacaaat gttcaaaatg gctaaaaaac agatgctctt ctactttttg
     2401 atagcttttt gtcactggta cagacaatcc caatataatg aaactcatca ctgacaaact
     2461 tagtaaagtt tcaagcaaag taaaagcttt aattgctaag acgggctttt tcatttttat
     2521 gtttgtcata atagtcttga taagcttttt cttgtttctc agtgatacta ccatctgcct
     2581 ttaactggct caaacttggt tttgagcctt gagataattc atatagttct gcttgattct
     2641 ccactaattt aacaacagcg gcattacctg tttctgtaac cctgtctttt tgcttgctta
     2701 aattaggcac aaataatagc atcaaaacac tgatgacaag aatcaccaat aacatttcta
     2761 gaagagtaaa tccttttagc ttcttgtgtc gtaagttgtt ccattgatta atcatcttaa
     2821 aaaatacctc ccatattttg gtaaattggt aataggattg ccgcataaat catgacgatt
     2881 gtcacagcaa ctactaaaaa aatagctggt tgaataagtt gtgtcacttg ataaagctga
     2941 ctaaaaaatt gttcccagct ttcctgggca tagatttcca actcagcccc cagtttcgat
     3001 ttgatttcac cgtattcaat cattaaactt aattcttttt taaaaaaagg gtaggtagct
     3061 actttaatgt gaaaggcttg
//
LOCUS       DAUCWU010000121         3053 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUCWU010000121 DAUCWU010000000
VERSION     DAUCWU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.121"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            39..410
                     /locus_tag="VGY24_001662"
     CDS             39..410
                     /locus_tag="VGY24_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ4284623.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            410..1108
                     /locus_tag="VGY24_001663"
     CDS             410..1108
                     /locus_tag="VGY24_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284624.1"
                     /translation="MAHLLQIHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            1118..1903
                     /locus_tag="VGY24_001664"
     CDS             1118..1903
                     /locus_tag="VGY24_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284625.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2030..2644)
                     /locus_tag="VGY24_001665"
     CDS             complement(2030..2644)
                     /locus_tag="VGY24_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ4284626.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIALFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            2736..>3053
                     /gene="ssrA"
                     /locus_tag="VGY24_001666"
     tmRNA           2736..>3053
                     /gene="ssrA"
                     /locus_tag="VGY24_001666"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1043 a          532 c          501 g          977 t
ORIGIN      
        1 ttagtacaaa tatctatatt actcagaaag gagaccacat gtcttggaaa tttgatgaaa
       61 aatctcctat ttatgcgcag atagcacaac atgtcatgat gcaaattatt agccaagaaa
      121 tcaagagtgg tgatcaactt ccaactgttc gtgaatatgc agaaatcgca ggtgttaatc
      181 ctaacaccat gcaacgagcg tttacagaac ttgaacggga aggaatggtt tattcgcaac
      241 gtactgctgg ccgttttgtg actgacgatc aaaaattaat tgctcgtaaa agacgagagt
      301 tagcaattag tgaattagaa tcatttatca ccaatatgac aaaaatggga tttagtcaca
      361 ctgagattat ccctgttttg acgagctttt taaaggagga ttcacaataa tggcacacct
      421 attgcaaata catcatgttt caaaatctta ccgtgaaaaa aaagctattg atgacttaac
      481 cattactatc ccgaacggta aaattatcgg attattaggt ccaaatggaa gtggtaaaac
      541 aactttaatt aaactcatta atggcctttt acaaccaaat aaaggagaaa ttgtcattga
      601 tggctaccgt ccctgtgttg agacaaaaaa aattatttct tatttaccag atactactta
      661 tctcaatgaa aatatgagaa ttaaggacat gttggagttc tttagtgatt tttacagcga
      721 ctttgacaaa tccaaagcaa caagccttct aagagattta gaactagacc ctgaagaccg
      781 cttcaaaact ctatccaaag gaaacaaaga aaaagtacaa ttaattcttg tgatgagtcg
      841 taaagccaga ctctatgtac ttgatgaacc tattggtgga gttgaccctg ctgcaagaga
      901 ctacatccta aaaaccatca ttaatagtta ttgtgaaaat gcttctgtta ttatctcaac
      961 ccacttgatt tcggacattg aacctatcct tgacgaagtc attttcttga aacaaggaag
     1021 acttttctta tctggcaatg ctgatgatct aagacaagaa taccagcaat ccattgattc
     1081 cctctttagg gaaacttata aggtttagga ggatatcatg tttggaaaat tattaaaata
     1141 cgaatttaga tctattggaa aatggtactt tgcgcttaat gctttcgtta ttgccatcgc
     1201 tgccattttg tcatttacga taaaactgtt tgctcaaagt aatagtgatg gactatttgg
     1261 agtactaacc aataagatgt tacctctgac attaggttta acttttggct ccctgatcgc
     1321 tggttccctc ttatcaacat tacttatcat tatcaaacgt tttagcaaaa gtgtttttgg
     1381 acgggaagga tacttaacgt tgactttacc cgttaattcg catcaaatta ttttatcaaa
     1441 actactagct tcttttattt gcagtgtttt caatactatc atccttgcat ttgctatcgc
     1501 tattgtaatt gtaccaatgt ttaacatcaa cgaactatta gaaggattct ttaatagttt
     1561 taagatggat tatttcatca atatgctaac tgtactagcc tatgtcctat tatcaacatt
     1621 tacgagtatc ttattaattt atctttccat ttctataggt caactttttt ccaatcggcg
     1681 aggcttgatg gcctttattg catattttat attagttatt ctgattagcg ttgctgcaac
     1741 atatgttcac agtcacatct ttaatattaa tacaagtgcc gatagtttcc catttactga
     1801 gcaaaaaaca atttatcttc ttattttgga acaatttatt gaaatgataa tgttttacct
     1861 cgccactaat tttattatca aaaataaact caacctacag taagcgttaa gactttcttt
     1921 taaactatga cacactatag tttaaaagaa agtttttttc agtgttcata gtaaataaaa
     1981 aaaccgtctt ccatcaaata gaagcggttt atcaaattaa caccaaacct taatgctgta
     2041 agaaccaaga tataacatct tttccaaaaa taaataaata actgtagccg ataatagaaa
     2101 ttggtttagt tattaaaaga atagttacaa agcgctttaa agacatgtcc gtcaatcccg
     2161 taatcataac cataatatca gctggagaaa caggcgatgc catacaaaaa ataaataatt
     2221 tttcatatcc tggtgtctcc aaacgccttt catacttgta aaatgttttg tcatttacaa
     2281 agagtaagat aaatttacga ccataagtct taacgagcaa gaagagggct atactgccaa
     2341 taatgatgcc tacataatta taaataaaac ctgttacagg gccaaagatt aaaaaacctg
     2401 caactgtcgt aaggcctcca ggaatcacag gaaaaacaat ctgtatgatc tgcacgacaa
     2461 taaaaacaaa cggcccccat aatctatagc gctgcactaa gtctttaaga gcattactat
     2521 cgtttaatat acctagtcta tacagccaaa aagcgaggaa aaaagtacca attagtgcaa
     2581 taattcctaa aattttgata attttttgcc ataatatata ggcttttgaa ttacgttttg
     2641 tcatagacta atcataccat aagctgtaaa attttgctat ttaaaaaaag ttttgataga
     2701 atagttagtg taagaaattg gttaattatc tatttggggt tgttacggat tcgacaggca
     2761 ttatgaggca tgttttgcgt cccatcggca gatgtaaatt gccagttaaa tataactgca
     2821 aaaaatacaa actcttacgc tttagctgcc taaaaaccag ctagcgtgac ttctacaaga
     2881 ttgcttgtgt cctgttagaa gtctcaaaat agcaagctac ggttacgaaa ttgtctagtt
     2941 tcgtgacaag agattgatag actcgcaaac taatggcttg agttatgtgt ctttagtttg
     3001 ttaaatgaag acataaccta tggacgtaga caaatatgtt ggcaggtgtt tgg
//
LOCUS       DAUCWU010000122         3050 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUCWU010000122 DAUCWU010000000
VERSION     DAUCWU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.122"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            52..1842
                     /gene="sfbI"
                     /locus_tag="VGY24_001667"
                     /pseudo
     CDS             52..1842
                     /gene="sfbI"
                     /locus_tag="VGY24_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
     gene            2130..2893
                     /locus_tag="VGY24_001668"
                     /pseudo
     CDS             2130..2893
                     /locus_tag="VGY24_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
BASE COUNT         1020 a          505 c          651 g          874 t
ORIGIN      
        1 tttcttgaca ataacgtggt aagctcatat atgtttttga gaggagagaa aatgaataac
       61 aaaatgtttt tgaataaaga agccggtttt ttggcacaca caaaaagaaa aaggcgattt
      121 gctgtcactt tagtgggagt cttttttatg cttttggcat gcgcgggtgc tatcggtttt
      181 ggtcaagtag cctatgctgc ggatgagaag actgtgccga atcatagtag tccaaatcct
      241 gagtttcctt ggtacggtta tgatgcttat agaggttggt ttctgagata tcatgattta
      301 aatgttaatt tggaaggtag cacgtcttac caagtttatt gctttaactt agtaagacag
      361 gaaccgagta aggttaatgg tttaagaaaa aattggttca aaaaagttga tggtgataat
      421 gctgtgttta aaaaatatgc agctaatcct agagtaattg atggagattt agaacgtaac
      481 attttaaatg taatttacaa tggatattca agtgatgcaa atgggattat gaaggggttg
      541 gaccggtaca atgctatttt agtaactcaa actgcgattt ggtattattc cgatagctca
      601 cccatctcgt tgtctgcagc tgaaacaatg tgggaaaagg aagctgaaag tggagatatt
      661 tcctgggagc aagtgaaact aatgcgtgaa gcgctgaaaa aattaattga tcctaaatta
      721 ggcgaaactg ttgtaaagaa gctacctaaa aattataaat taaatatttt tgtttcaaaa
      781 gatgaatcaa ttcaaaatct tttaagtgct gaatatgtac cagatagtcc tccaatgcca
      841 ggtgaagtac ctaaagagta gagtccaaaa gaagagaacc ccaaaattcc agatttagat
      901 gggcatgaaa tcccagaagt tccacaagaa ccttcagacc cagttatccc tccagttatt
      961 ttggatggtg aagaagtccc agaagttcca agcgagagct tagaacctgc gcttccccca
     1021 ttaatgccag aactagatgg tgaagaagtt ccagaagttc caagcgagag cttagaacct
     1081 gcgcttcccc cattaatgcc agagctagat ggtgaagaag ttccaagcga gagcttagaa
     1141 cctgcgcttc ccccattgat gccagagcta gatggtgaag aagtcccaga agttccaagc
     1201 gagagcttag aacctgcgct tcctccatta atgccagagc tagacggtga agaaatccca
     1261 gaagttccaa gcgagagctt agaacctgcg cttcccccat tgatgccaga gttagatggt
     1321 gaagaagtcc ctgaaaaacc tagtattgac ttacctattg aagttcctcg ttatgagttt
     1381 aacaataaag accaatcacc tctagcgggt gagtctggtg agacggagta tattaccgaa
     1441 gtctatggaa atcaacagaa ccctgttgat attgataaaa aacttccgaa tgaaacaggt
     1501 ttttcaggaa atatggttga gacagaagat acgaaagagc cagaagtgtt gatgggaggt
     1561 caaagtgagt ctgttgaatt tactaaagac actcaaacag gcatgagcgg tttcagtgaa
     1621 acagtgacca ttgttgaaga tacgcgtccg aagttagtgt tccattttga caataatgag
     1681 cccaaagtgg aagagaatcg ggaaaagcct acaaaaaata taacacctat ccttcctgca
     1741 acaggagata ttggaaatgt tttggccttt cttggaatcc ttattttgtc agtactttct
     1801 atttttagcc ttttaaaaaa caaacaaaac aataaagtct gacgtaaaag tgttccataa
     1861 gaaataatgc gcaaatatgc tgaatagtgg ataactgcta cagtcaagac aagtgttaat
     1921 tcaaaaggta ctacttatac atctgataat aataaggtga cagataattc agagcaagta
     1981 ggatggctaa aaaatgatgt attctatgta taatagactt acaagaaatc aagttattgt
     2041 taaacgtcgt aaagcttaac gctttcaaaa gtcgaaatca atcgagactg tctttaggca
     2101 gtctcgattt ttattgataa ggaactgcaa tgacagaaag actaaaaaat ctagggatac
     2161 tcttattatt tttattggga acagccattt ttctttaccc tacgctaagt agtcagtgga
     2221 atgcctatcg tgatcgtcaa ttgttatcaa cttatcacaa acaagtgact caaaagaagc
     2281 cctcagaaat ggaggaggtg tggcaaaagg ctaaggcgta taacgctagg cttgggattc
     2341 agcccgttcc agatgctttt tcatttaggg atggcattca tgataaaaac tacgaaagcc
     2401 tacttcaaat tgaaaacaat gatattatgg gatatttgga agtgccttac cagttggtgg
     2461 cgacgggact cacacggtta tttctgctca tagaggcctt ccatcggcag aaatgtttac
     2521 taatttgaat ttagtgaaaa ggggagatac tttttacttt tgtgtgttaa ataaagtatt
     2581 ggcttataaa gttgatcaga tactaactgt tgaaccagat caagtcacat ctctttctgg
     2641 ggtaatggga aaagattatg ctactctggt tacctgcaca ccatatggtg ttaataccaa
     2701 gcgtctattg gtcagaggtc atagaattgc atatcattac aaaaagtatc aacaggctaa
     2761 aaaagcaatg aaactcgtgg ataaatcaag aatgtggaca gaagtagtat gtgctgcttt
     2821 cggggtggtt atagctatta ttttagtatt tatgtattca aagatgactg ataaaaaagt
     2881 gattaaaaac taacttgccc tgaaccttct ttatcgtatt gaaactgtca taaaagtaaa
     2941 ataatctggt agttactatc ccctttgatg aagtttaatt aaatctcatc aagagaaaaa
     3001 tattaggcga ttacaaatga ttttcccaaa aattattttt tggaacgcat
//
LOCUS       DAUCWU010000123         3031 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUCWU010000123 DAUCWU010000000
VERSION     DAUCWU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.123"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..577
                     /locus_tag="VGY24_001669"
     CDS             <1..577
                     /locus_tag="VGY24_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ4284627.1"
                     /translation="INPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLT
                     SYARLLSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYF
                     IWSNGTNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVK
                     AIQNDLKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            756..1451
                     /locus_tag="VGY24_001670"
     CDS             756..1451
                     /locus_tag="VGY24_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ4284628.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1453..1794
                     /locus_tag="VGY24_001671"
     CDS             1453..1794
                     /locus_tag="VGY24_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ4284629.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSMTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
     gene            1787..>3031
                     /locus_tag="VGY24_001672"
     CDS             1787..>3031
                     /locus_tag="VGY24_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ4284630.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVCIVSLVFIMYYDIGHSKKFQKLMFSELLSNISFQNSLKLLKESFP
                     LFLNGFLIIYIYTQPKYAIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHIT
                     QMAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSMLYGTNLTDYWVD
                     FMLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAA
                     LSFLITMLVWLGLSIMIYLFIMN"
BASE COUNT          981 a          433 c          520 g         1097 t
ORIGIN      
        1 aattaatcct agtgaaagcc aatttttctc agttattaca atgcaaaatc atattccttg
       61 gtcatccgat tatcctgaag aaattgttgc tgaaggaaaa aatttcacgg aagaagaaaa
      121 tcacaaccta acaagttatg ctcggttatt atcgtttact gataaggaaa caagagcatt
      181 tttagaaaaa ttaacacaaa ttaacaagcc tatcacagtg gtgttttacg gagatcattt
      241 acccggttta tatcctgata gtgcttttaa caagcatatt gaaaataaat accttactga
      301 ttattttatt tggagtaatg gtactaacga gaaaaaaaat catccgctta tcaactcaag
      361 tgattttact gcagctttat ttgagcatac tgattcaaaa gtatcacctt actatgcttt
      421 gttaacagag gtactgaata aagctagtgt cgataaatca ccagatagtc ctgaagttaa
      481 agctattcag aatgatttaa aaaatatcca atacgatgtg actataggaa aaggttacct
      541 tttgaaacac aaaacttttt ttaagatatc acgttaagaa aaatgctagg ctgtccgagt
      601 atagacgagc atcaaggttt ggtaatttgc tcaaatgatt gtcaacagag tatctagggc
      661 caggtttaaa agattataat tcttgaggtc ttattcgttt ataaaaaatg aatactcttt
      721 taaaatctta attgaaatga ataggagaag ttttcgtgaa aaagttaatc attattcctg
      781 cttacaatga aagcagtaat attgtcaata ctatacgtac tattgaatca gatgccccgg
      841 attttgacta tatcattatt gatgattgct caacggataa tacgttagca atatgtcaaa
      901 aacaggggtt caatgttatt tctttgccca ttaacctggg aattggcggt gcggtgcaaa
      961 ctggctatcg ttatgcacaa agatgtggat atgacgttgc agttcaagta gatggagatg
     1021 gtcagcacaa tccatgctat ttggaaaaaa tggttgaggt attagttcaa tcttcagtaa
     1081 atatggtaat tggatcacga tttatcacaa aagaagggtt tcagtcatca tttgctcgac
     1141 gtatcggtat aaagtatttt acttggctta ttgcgctact aacaggaaaa aaaataacag
     1201 atgcaacgtc aggtctaagg ttaattgacc gttcattgat tgaacgtttt gctaatcatt
     1261 atccagatga ttatcctgaa cctgaaacag ttgttgatgt attggttagt cattttaaag
     1321 tgaaagaaat tcctgttgta atgaatgagc gacaaggcgg tgtgtcatct atctcgttga
     1381 cgaaatcggt ctattatatg attaaagtga ctttagctat tttagttgtt agattgaaag
     1441 gaaatcgtta aaatgacggt aacagccagt ttatttataa gttttattgt attaaccttt
     1501 gttttctttt tgattaactt aatcaaaaaa gacaaactag cgattaagta ctctttattg
     1561 tggtttattt tagctttatt aattttacta tttacgtggt tacctaacat attaaataaa
     1621 atgtctcact tcctgggtat tcattcacca accaatatgt tattcttttt aggtttttgt
     1681 ttgagtttag ctattatttt ttcaatgact aataatatct cacttcaaaa tgataaagta
     1741 aaacgtctga cccaagaagt ggcccttatg aaaaaagaga aaaccaatga ttaatccttc
     1801 aaaacaaaac caaacgattt ttttatggaa tatgttagga agtttatcaa cagctgtaat
     1861 atcagttata ttacttatgg tggttactag gttactaact tcggctgatt cagatattta
     1921 cgcctttgcc tattcttttg ctaatatgat ggtagttgtt ggactttttc aggttcgtaa
     1981 ttatcaagca acagatatta atgaaaaata ttcctttagt cagtacttag tggcaagact
     2041 gatgacttgt ttattaatgt tagccataac agtaatttat ttaacgttaa ctaagacaga
     2101 tagctacaag agtacaattg tctttctagt ctgtttctat agatcgacag atgctttttc
     2161 ggatttatat cagggaatgt tccaacaaca tgaacgactg gatattgcag gtaaatcgct
     2221 agcctacaga aatactctta tttttatggt gtacacagca ataatattat attccaaaaa
     2281 tcttacttta gcattagtag ctgtctgtat agtttcatta gtttttataa tgtattatga
     2341 cattggccat tcgaaaaaat ttcaaaagtt gatgtttagt gaattattaa gtaatatttc
     2401 atttcaaaac agtttaaagt tattaaaaga aagttttcca ctttttctaa acggtttttt
     2461 gattatctat atttatactc aaccaaaata cgctattgaa ctaatgacga ctttaggtga
     2521 agttgctttg ggctctcaaa caatttttaa tattttattt atgccagcct ttgttatgaa
     2581 tttgctaatt ttatttttta gacctcatat tacacagatg gctattgctt taatcagagg
     2641 tcaaataaaa gagtttaata agatacaagt tcaattgttt gcttatttag gagttttttc
     2701 actgatagcg ttagttggaa gtggtttgtt tggtattccc tttttatcaa tgctatatgg
     2761 tactaacctt acggattatt gggtagactt tatgttgatt atgctaggag gctcgattgg
     2821 tagttttgcg acagttatcg ataatatctt aaccgctatg agaaaacagc aacttcttct
     2881 tattccatat acaggaggat ttttgatttc gttattaatt accaatcttt ttgttatgaa
     2941 atatcatatt ttaggagctg ctttgagctt tttaataaca atgttggttt ggttgggatt
     3001 atccattatg atttatctat ttattatgaa t
//
LOCUS       DAUCWU010000124         2954 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUCWU010000124 DAUCWU010000000
VERSION     DAUCWU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.124"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..545)
                     /locus_tag="VGY24_001673"
     CDS             complement(<1..545)
                     /locus_tag="VGY24_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEQ4284631.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGENIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLI
                     ALINLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKI
                     NVYFAMIINIISPLIIGTSFSL"
     gene            complement(551..1240)
                     /locus_tag="VGY24_001674"
     CDS             complement(551..1240)
                     /locus_tag="VGY24_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ4284632.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            complement(1246..>2954)
                     /locus_tag="VGY24_001675"
     CDS             complement(1246..>2954)
                     /locus_tag="VGY24_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEQ4284633.1"
                     /translation="LNFNIKIINNDVKLYLGANIGSGQAGRSFGRFYGLSETVRETIK
                     NLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDIYIRYS
                     DGKFHFTTIDGKNELKFTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWDILAQ
                     ERVYIPEILFEDITIATAQWNLSVIREEIIHQKIFTGKKDIFNKFRKTYKIPKDIILK
                     YADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDNLGNYNTEIVVPILK
                     ESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSEL
                     FSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSS
                     YKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCATYLVLYYLNHFFKD
                     KETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSY
                     MKEYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIE
                     WE"
BASE COUNT         1027 a          525 c          334 g         1068 t
ORIGIN      
        1 agagaaaaac ttgtaccaat aattagtgga gatataatat taataatcat agcaaaatac
       61 acattaattt ttcttgatag ccataaaata aacgggattt gccatagtgt agtcaaccaa
      121 attaacaaga tagttaatag tatcctacct atactaataa gacttggagt aggtaggaca
      181 aaactaatta caataagcat taagcttaaa atcatactaa ttattagatt tataagagct
      241 atcaatagaa ctctggcata ttctaccttt tttaaatcaa catcccttga taaaaaaaca
      301 gaatacttat tagaatctaa ttctaattta tttaacaaac cagaatttac agcaataata
      361 acattaatcc aaataactcc ccactgattt actgttgttt ccgcagttaa acgtaggata
      421 ttttcaccac ctaatgctga aaacaataaa gataacatca cacaaataat agggaaaata
      481 agaatcaact tgctaatact cgtatttttt atttttaata aatttgataa aaaaatttgt
      541 accatagccc ctacctcctc ttttcaaata aaatttgatt attgaataac tcttcaagat
      601 tctcagagcc gtcatattct ttttccagaa taatctttcc ttcatagata aatccaataa
      661 aatcagctag atgttcaact tctgacagta tatggcttga aatcataatt gtcattcctt
      721 ctgatttaaa tgactctata atttccctta attcttgaat tccaattggg tccaagccat
      781 tagtaggttc gtctaatata agtagtttag gtgaatttat taaagagata gctataccta
      841 accgttgctt cattccaaga gaaaattgtt ttactggtct cttgtcaata tttcctagac
      901 caactttatt taataatgga agtatagtgc tttctgaaac accaagcatt gttgtaacta
      961 ccttcatatt atcgtaagca ctcaaatttt tataaagtgg tggttcttca ataatactcc
     1021 cgatttttcg cagatcccga cgtgaccatt ctctaccttc aaaaataata gaaccacttg
     1081 tcttactaac taagcctgtc aaaattttca taatagttga ttttccagca ccatttggac
     1141 cgattaaagc atacactttt cccttaggaa tattcatatt gacattattc aatatggttc
     1201 tcttaccata agattttttt agattttgta ttttcaacat attagttact cccattctat
     1261 atattgctga gcgtttagtg tatagtaagc aaaacaatac actttctctt caaactctct
     1321 atcaatcccg tttaatcgat tcattgatag atgtaaaaaa cttcctacta aattgaattt
     1381 agtattatag atatcatttg taccaccaaa tcgattatac tctttcatat aagacatgag
     1441 atatctatcc atttcttgga aaaaatcata tctatcaaga gctgaggtga ctccattcaa
     1501 aatcttcata tactcaatag gtaaatcaat tggcaaattt ttgaaatctt tcttatactt
     1561 tccggtataa ttctcaaata agaaagagcg ccttgtttct ttatctttga aaaagtgatt
     1621 tagataatat agaaccaagt aagtagcaca aactaatctt tcatcatctg aaaattctat
     1681 tgtattatta agaaataaga tagattcttt acaaaaaatt tcctctgcat aacttattaa
     1741 atgaggtcct ccatatctat ttacttctgg tttatagcta ctcacttcta tgtcagagat
     1801 aatacaactc tcaatcagtt gaggtaatag atcttgaact actgacaaat acaaagaaaa
     1861 gttattttca atcgaatcac aatttatccg aattcttata tgatcattgg tatcattata
     1921 tctcatataa aacatgttac tttggctaaa cagttctgat ggcaggttta gccaaaattt
     1981 tttccagtta tttagtaatt cttcttgtcg ctcttttgga ccataaaatt tgaaaaatag
     2041 ccagtcacaa aatggtaaat atattttctt aacttcattt ttaaattgaa cattatcgtt
     2101 tgtattgttt attccattag attccttaag aattggaact actatctctg tattgtagtt
     2161 ccctaaatta tccttaaaaa tagactctcc ctgctcaatt cctctcaaag aaacaaacct
     2221 atatttatta atgtttttaa ataaaatctc taaatcacta tctatagata aatctaacct
     2281 caattcatta tctgcatatt tcaaaataat atctttaggt atcttataag tctttctaaa
     2341 cttattgaat atatcttttt taccagtaaa tattttttga tgtataatct cttctctaat
     2401 tactgataaa ttccactgag cagtggcaat cgtaatgtct tcaaataaaa tttcaggaat
     2461 atatactctt tcctgagcca aaatatccca aggaaataat gaccaatgcg ataaaccgta
     2521 ctcaaactca gataaatcaa ccaacaatct caaaaccctg ctttggctat cggaatttag
     2581 catattattc atggtaaatt tcaactcatt tttcccatcg atagttgtaa aatgaaactt
     2641 accatcacta tatcttatat aaatatcctc caatcgcagt tcgttctctg aatcccatga
     2701 tgtggtaaaa aatgaagtat tataagactc atcactataa ttttgaatta cattcgcaag
     2761 tcttatctgt ttaggaacaa atgacaactc tacattcgta ttattttgat ggtttaaatt
     2821 cttaattgtt tctcttactg tttcgcttaa tccatagaat cttccaaagc ttctacctgc
     2881 ttgtccagaa ccgatattag ctcctaaata taacttcaca tcattattta ttatttttat
     2941 attaaaattt aatt
//
LOCUS       DAUCWU010000125         2882 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUCWU010000125 DAUCWU010000000
VERSION     DAUCWU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.125"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..2229
                     /locus_tag="VGY24_001676"
     CDS             <1..2229
                     /locus_tag="VGY24_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ4284634.1"
                     /translation="ICQLDEKNYSKTISFQDINYQLALEEDRDLIFNQFANFLNSFDP
                     SVHIELSYVNQLGRNKDLQDAIKIADKGDFYDDVRKEFREMLKLQLAKGNNGLKKMKY
                     ITFTTEADNLEQARAKLNRLEVDILSNFKSMGVRAESLDGEERLRLVHDMLNPDKNFD
                     FSYKELKKKESTKSHITPNIFNFAPANNFKFGKFIGAVSHFQILASELSDRMLSEFLD
                     IDDNIYVSFHIDVVEQAEAIKLIKRKNTDLDRMKIEEQKKAVRAGYDMDIIPSDINTF
                     GADVKSMLSDLQNRDERLFVVTIVMMNFARTNQRLENTIAQISSIANRHNCQVKRLSH
                     KQEQGLVSVLPLGVNQVEIKRFLTSSSTAVFMPFTTEELFIDSANSLYYGLNALSQNL
                     IMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEGEYSNLVKQF
                     NGEVIKVSAKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGSGLTAAEKSV
                     IDRCLPKIYQKYFEDPKPENMPILGDLYDMLLSQEEGVGRKLATEMEIYVKGSLNVFN
                     NRSNVDLNRQLLCFDIKELGTQLKKIGMLVIQDQVWNKVSLNRGSKSTRYYIDEFHLL
                     LKDPQTASYSVEIWKRFRKWGGIPTGITQNVKDLLTSQEIENIFDNTDFVLMLNQASG
                     DRDILAKKLKISPYQLNYITNSNAGEGLLFFGNTIVPFIDKFPKDTMLYKLMTTKPEE
                     AK"
     gene            2237..2882
                     /locus_tag="VGY24_001677"
                     /pseudo
     CDS             2237..2882
                     /locus_tag="VGY24_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839222.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1175 a          394 c          511 g          802 t
ORIGIN      
        1 atttgccagt tagatgaaaa aaattattca aagacaattt catttcaaga tataaattac
       61 cagttggcat tggaagaaga tagagattta atctttaacc aatttgccaa ctttttaaat
      121 tcttttgatc caagtgtcca cattgaacta tcatatgtaa atcaattagg aagaaataaa
      181 gacctgcaag atgcaattaa aattgctgat aagggagact tctatgatga tgtaagaaaa
      241 gagtttaggg agatgttaaa gcttcaactt gcaaagggaa ataacggact taaaaagatg
      301 aagtatataa cctttaccac agaagccgat aacctagagc aagcaagagc aaagttaaat
      361 agacttgaag tagatatttt atctaacttt aaatctatgg gagtaagagc agaaagcctt
      421 gatggagaag aaagactaag acttgttcat gatatgttaa atccagacaa aaactttgat
      481 ttttcatata aagaattgaa gaagaaagag tctacaaagt cacatataac tcccaatatc
      541 tttaattttg caccagcaaa taattttaaa tttgggaaat tcattggagc agtaagtcat
      601 tttcaaatac ttgcaagtga gctatcagac agaatgttat ctgagttttt agatattgat
      661 gacaatattt atgtatcatt tcatatagat gtagttgaac aagcagaagc tattaaactc
      721 attaagagaa aaaacacaga cctagacaga atgaaaattg aagaacaaaa gaaagctgtt
      781 cgtgctggat atgatatgga tattatccct tcagatataa atacttttgg agccgatgtt
      841 aagtccatgt tatctgactt acaaaataga gatgaaaggc tctttgtagt taccatagta
      901 atgatgaatt ttgctaggac taatcaaaga ttggaaaaca ctattgctca aatttcatca
      961 attgcaaaca gacataattg tcaggtaaaa agattatctc ataagcaaga acaaggactt
     1021 gtttctgtct tacctttagg agttaatcaa gtagagataa aaagattttt aacttcatca
     1081 tcaacggcag tatttatgcc ttttacaaca gaagagctat ttattgattc agcaaattct
     1141 ttgtactatg gattaaatgc ccttagccaa aacttgatta tggcagatag gaagaaacta
     1201 aagaacccta acggattaat attaggaaca ccaggttctg gtaaatcttt ctcagcaaag
     1261 agagagatgg caaatgcaat tcttgtaaca gacgatgatg taattatttg tgatcctgag
     1321 ggcgagtatt caaaccttgt aaaacaattt aatggagaag ttattaaagt ttcagcaaag
     1381 tcaaaggatt acctaaatcc actagatata aatatgaact atggagatgg agacgcacct
     1441 ttaaaggata aggcaaactt cataatgtct atgcttgaac ttgtagtagg aggatctggt
     1501 cttactgctg cagaaaaatc tgttatagat aggtgcttac caaagatata tcaaaaatat
     1561 tttgaagacc caaaaccaga aaatatgcca atattaggag atttatacga tatgctccta
     1621 tctcaagaag agggagttgg taggaagctc gcaacagaaa tggaaattta tgttaaagga
     1681 agtcttaatg tctttaataa taggtcaaat gttgatctaa ataggcaatt gctctgcttt
     1741 gatataaaag agctaggaac acaacttaaa aaaataggta tgcttgtaat tcaagaccag
     1801 gtttggaaca aggtttcctt aaatagagga agcaagtcca caagatacta tatagacgag
     1861 ttccaccttt tattaaaaga cccacaaact gcttcatatt cagtagaaat ctggaaaaga
     1921 tttagaaaat ggggtggtat tccaacaggc ataactcaaa acgtaaaaga cctattaaca
     1981 agtcaggaaa ttgaaaatat ctttgataat acagactttg ttctaatgtt aaatcaagca
     2041 tctggagata gagatattct tgctaagaaa ttaaaaatat cgccttatca gctaaactat
     2101 ataactaatt caaatgcagg tgagggatta ctattctttg gaaatactat tgttcccttt
     2161 atagataaat tccctaaaga caccatgcta tataagctga tgacaacaaa gccagaagaa
     2221 gctaagtagg tgtgatatgg ataaaaaact aaaaaaagat tttcaaaaga aaattatacg
     2281 aaatagggac gctcctgaaa agaatcttga aagtaaactt gttcattcag atgattatac
     2341 caataagatt attaagacta aggataggtt cggagataaa atttcagaaa aagaatctaa
     2401 acttattcat gagaatgtat tagctaaaga tcaaaaacaa gataaactaa aagattttca
     2461 gaagtctaaa aacaaggaaa gaattagaaa agaagtttta gataataagg ataatgttga
     2521 agaaacaaaa caaactaatc cttaaataag gacagatgaa agctttaaac ttgatgaaga
     2581 cttagatgta gacttcaaaa aggtaaattt tgatagtgaa aacagtagaa atattaattc
     2641 taataaatta acaacagatg atatttcagc taagactcaa ccaataagca ataagaagac
     2701 atcaagtaaa agacaagttt ttaaaaatta cgaggataaa tttatccata gtaaggataa
     2761 attccaagac aaaatcaacg agaaagagtc taagcgaatc cagacaagcg aagataaacc
     2821 tatcgaagca aagaaaagca agagaatata cagaaaagat aagcttgtta aagatgaagt
     2881 aa
//
LOCUS       DAUCWU010000126         2821 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUCWU010000126 DAUCWU010000000
VERSION     DAUCWU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.126"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            231..395
                     /locus_tag="VGY24_001678"
                     /pseudo
     CDS             231..395
                     /locus_tag="VGY24_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            432..728
                     /locus_tag="VGY24_001679"
     CDS             432..728
                     /locus_tag="VGY24_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEQ4284635.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            729..1145
                     /locus_tag="VGY24_001680"
     CDS             729..1145
                     /locus_tag="VGY24_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEQ4284636.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1165..2709
                     /locus_tag="VGY24_001681"
     CDS             1165..2709
                     /locus_tag="VGY24_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ4284637.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKLIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKTRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHLPQ
                     YYVENSHEAIIPKELFLQVQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRTYWNIHGRKEFVWRCVTRIEQGPGVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
BASE COUNT         1005 a          484 c          614 g          718 t
ORIGIN      
        1 taggtgatga aaaaagaacc cctttctgat ttagcggaca aatactttga cctaatctca
       61 gaagatataa aagaaaaaat aataaaatat ccgatttata ctttgtataa agaactaaag
      121 tagttagcaa atcatttaca gcaaatcact atgttccaca taccatttta catacttacc
      181 ttatccacga cacatgttga gatagtatcg aagataaaga aagttgctcc atgttgtttt
      241 tgcgagatgg ggaaatcaaa gccacattga caaccatgat gaataagctt gctttcagtc
      301 ataagctaat cctagaacca cttttcaagt cagttagcca aatcgatgaa gaaagcgacc
      361 gtgaaagaat ggacgctatt gataagctaa tggagcaact attggaagaa cgcaataccc
      421 ttattgcact gatgtccaaa ggtttcctag aacctgctct ttttaatcag gaacgaaatg
      481 ttttagatag tgagattaaa aatcttacta ccgaaaaaac aaacctggta acgaactctg
      541 cgagtggggt tttgcgagca aacgagataa aggacctcat taattatgtg tcagcagata
      601 attttaatgg tgactacacg gaagaactat ttgaagaatt tgtagtgaac atcattgtaa
      661 attccaggga tgagctgaca ttcaatttga aatgcggtct ttccctgaaa gaaaaggtgg
      721 tgagataaat ggcatatatt ccatatggat acaaaattca agatggagtg gttactgtcg
      781 atgaaaaggc agcaggtcaa gtaaaggtat tctttgagaa atacatatca ggactatccc
      841 ttacagtggc tggcgaacag gcaggtattg aaaagacaca ttctgtgatg gggcgcattt
      901 tgaaaaacgt caactacctt ggaaatgata cgtatccagc aattattgat aaagagatat
      961 ttgataaagc tgaagaagtt agagataaac gtgcaaagga tttaggacga gtggtagagc
     1021 ttgccgcttt cacctctccc cctcccaaag aacgattcaa aatgaaaaag gcagataata
     1081 agatgccagt ggatcctttt gaacgagcag aatacttata cagtctgata gaaagcgagg
     1141 aataaagtga cagagaaaaa tataatggtt attcctgctc gtaaaagagt aggaagtaca
     1201 gccgcaaaag aaaagataaa gaaacttcgt gttgctgcct attgccgtgt ttctacagaa
     1261 acagaagaac aaaattctag ctatgaggtt caggtctcac actatacaga gtttataaag
     1321 aaaaataatg aatgggagtt tgctggcatc tttgcagatg atggtatctc tggtacgaac
     1381 actaaaaagc gtgacgaatt taatcgcatg attgcagagt gtatggacgg taacatcgac
     1441 atggttatta caaaatccat cagccgattt gcgcgtaaca ccctagactg ccttcaatac
     1501 attagacagc tcaaggataa gaacatatca gtttattttg agaaagagaa catcaacacc
     1561 acggatgcca aaggtgaggt tttgctgact attatggcat ctttggcaca gcaggaaagc
     1621 cagagccttt cacaaaacgt taagcttggg ctacagtacc gataccaaca aggaaaggtg
     1681 caggtcaacc ataagcgatt tatgggctac acgaaagatg aagatggaaa tttaactatt
     1741 gtccccgaag aagctgagat tatcaaactc atctaccggg aataccttga gggtcagagt
     1801 ctagtgggca ttggtcgagc ccttgaaaag gatggtattt taacagcagc gggaaaaaca
     1861 agatggcgac cggaatcagt taaaaaaatc ctccaaaacg aaaaatacat cggagatgcc
     1921 ctcttacaaa agactgttac tgtagatttt ctaaccaaaa aacgagttaa gaatgaaggc
     1981 catcttcccc agtattatgt tgaaaatagc catgaggcga ttattcctaa agagttattt
     2041 ttgcaggtgc aggaagagat tcatcggaga agcaatatct acacaggaga aggcaagaac
     2101 aaaagaattt atagtagtaa atacgcttta agtgccatta ccttctgtgg agattgtggc
     2161 gatatttata ggagaaccta ttggaatatt catggcagaa aagaatttgt ctggcgatgt
     2221 gtgactagaa tcgagcaggg tcctggggtc tgtaagaacc gaaccgtaaa agaagatgaa
     2281 ctctatggtg cagtaatgac tgcgattaat aagctacttg caggtggcaa taacatgata
     2341 aagacactgg aagaaaatat tcatgcggtg attggcgaaa cgacagagta ccaaatttca
     2401 gagattaaca actcactaga ggaaaaacaa aaagaactaa tcaagctggc gaacaagggt
     2461 caagactatg accacttagc agatgagatt gatgagctga gagacaagcg acagatcctt
     2521 ttagtagaag atgcctccct cagtggcgag aacgagcgaa tcaatgagct gattgaattt
     2581 atccgcaaga acaaattccg caccttagaa tatgatgata agcttgtaag gaagataatc
     2641 cagagcgtta cagtctatga aggccacttc gtcatagcct tcaaatctgg catcgaaatg
     2701 gaaatatgaa aaccagaagt aaatagccca tgactctaca gtagagttgt gggttttctt
     2761 ttgcttgctg attataataa tattttaata actattgtgt ttattaacca aatggtttat
     2821 a
//
LOCUS       DAUCWU010000127         2772 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUCWU010000127 DAUCWU010000000
VERSION     DAUCWU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.127"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            99..170
                     /locus_tag="VGY24_001682"
     tRNA            99..170
                     /locus_tag="VGY24_001682"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Glu,seq:ttc)
     gene            204..277
                     /locus_tag="VGY24_001683"
     tRNA            204..277
                     /locus_tag="VGY24_001683"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Asn,seq:gtt)
     gene            complement(774..>2772)
                     /locus_tag="VGY24_001684"
     CDS             complement(774..>2772)
                     /locus_tag="VGY24_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ4284638.1"
                     /translation="QNYYFGYMIALFCILYALVCLLRLNDFNKMFIAFVRFTAVSICA
                     ALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIPAKLSIGVYDTTKFNALPMI
                     YVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFYLQPLDLFWQGMHSPNMFLH
                     RYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIILTSLPYSFSQQYNFLPLTLF
                     LLSVFLLLGYTISLFSFRNSQIPSTFISAFILIFSLLESGLNTYYQLQGINKEWGFPS
                     RQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDSMKFNYYGISQFSSVRNRLS
                     SSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSEGPLNKFGFTKLKTSGNTTL
                     YQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSGKSLTYFNLQPAQLISGANQ
                     FNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIFSNPDAKEVRIQTDNHNFIY
                     TTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHFYSLSIESYLEAMNSIKQKN
                     VHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGKNLPVKKAQGGFLSVTIPKG
                     KGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSKLL"
BASE COUNT          985 a          383 c          492 g          912 t
ORIGIN      
        1 ttttaaaaaa atcaaatttt ttttaaaaaa agtatagaca aaaaaaatca atctgctata
       61 atagtaagag tgttaaggag atatcctcca aaatttctgg tccgttggtc aaggggttaa
      121 gacaccgcct tttcacggcg gtaacacggg ttcgaatccc gtacggacta tcgcaattgt
      181 cattttggca atttttttct ttttccggca tagctcagtt ggtagtagcg catgactgtt
      241 aatcatgatg tcgtaggttc gagtcctact gccggagtga taagatattt cctaagataa
      301 tcttaggatt tttttattta gtaaaacaga tactcaggtc aaatgctcaa tttactaatt
      361 attaaaccac taaatacagc attgtgaact aacaggattg tattttgcat taaaatagaa
      421 gttctgttct ttaattgtat agatgataac attaagttta attacaaaca ttaactgaag
      481 cttaattttt tacattttga aagcatgtct ccaatttaat actgatgaat ataagattat
      541 tgtaaggagt caaaagattg aaatcgtatc agcgatgatt attgtcattt caatctttga
      601 aagagagtag tttaacatta accctcacga aaaatctgac ctttatatta tttgagttta
      661 ttagtaaaat cgataaaagc atctcaagta ttagaatatt tgtctagctc ttgagatgct
      721 tttagtatat tgactcattt gccaaattga taaaaaaatc tgattttttt ggactaaagt
      781 aatttagact ttatatctat gtacttatac aaaagcatat aagcgataat tcctacgcaa
      841 gatagagata accctaattt aaaaccatta ggaataaagg taaggataac acgtcccttt
      901 cctttaggaa tagtaactga tagaaatcct ccttgtgctt ttttgactgg aagatttttc
      961 ccatcttttt gtgctgacca acctttatcg taaggaagtg taaaaataag agaacctttc
     1021 gtttttgaat tataatcagt gattaccgta ttacttttag cgtaagtatg aacatttttt
     1081 tgtttaatac tattcattgc ttcaaggtaa gattcaatag acaaactata aaaatgaggt
     1141 tccttaaaac taatttgttt attttttgga aaaacaaacg aaaatgtagc aactttggca
     1201 tcggcgaaat atcctaaatc aaaaaaagag taagcgttat ctgtagtata tatgaaatta
     1261 tgattatctg tctgaatacg cacctcttta gcatcaggat ttgaaaatat aatattgggt
     1321 atgctaacat agagttgact atgtttaggg atgttaattt gataattaag ggtaacggag
     1381 ttttgataat cagaagcttg tgcagatatt tgtccgttaa attgattagc accagaaata
     1441 agttgagctg gctgtaagtt aaaataggtt aaagatttcc cacttagttg gttaagtaat
     1501 ttggtttggt tatcaagggt attgacattt aggttgacat ctttgtaaac attacgtgtt
     1561 aatatagcta aagggctact atagtgattt tgataaagag tagtattccc gctagttttt
     1621 agttttgtaa atccaaattt atttagaggt ccttcgctaa gattatattt tataccaagt
     1681 agactgtcca taataatagt attgttttgg tatctaaggt ttaaatttgt gcctttagac
     1741 tgaaatccca atcgatccaa taaagaacta gatagtctat ttcttacaga ggaaaattgt
     1801 gaaatgccgt aataattaaa tttcatgcta tcgttccctg tttggggaag tagcctttcc
     1861 attctaaaaa aaggttgact attttttgac acagagttga caaggttgtt aatatctttt
     1921 aattgactat tatatatctg tcgtgatggg aatccccact ccttattaat tccttgaagc
     1981 tggtagtagg tgtttaaccc tgattcaaga aggctaaaga taagtatgaa agcagaaata
     2041 aaagtagatg ggatttgaga atttctaaac gaaaatagtg aaatagtata acctaataat
     2101 aaaaaaacac taagtaaaaa aagagttaaa ggtagaaaat tatattgttg agaaaagcta
     2161 taaggaagag atgtcagtat aatgaggaaa ataaaagcaa aacctgcttt tatttgagtc
     2221 acttctttta gtcgagagag agtctcacat gcgagtaata ggataactat ggaaaaagac
     2281 caagcgtagc gatgcaaaaa catatttggt gagtgcatcc cctgccaaaa aagatcaagt
     2341 ggctgtaggt aaaaacttat tataataaaa gttaacaagc aggcattggc taattttatt
     2401 tttaaaggga tactttctaa agtaaaataa ataacactaa gcattagggg aaataatcct
     2461 acgtaaatca taggcagagc attaaacttg gtagtatcgt acactcctat tgagagctta
     2521 gcaggtatat ccaaaaacca agcattgttc gtaactaact gttttaccgg ggatagattc
     2581 tctccataag ttgacaaatc tagataggta ggaagtatta ctagagcact tgttaaagca
     2641 gcacatattg acacagctgt aaacctaaca aaagcgataa acattttgtt aaaatcattg
     2701 agacgtaaaa gacaaactaa ggcgtaaaga atacaaaaaa gagcaatcat gtagccaaag
     2761 tagtaatttt ga
//
LOCUS       DAUCWU010000128         2630 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUCWU010000128 DAUCWU010000000
VERSION     DAUCWU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.128"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            19..939
                     /locus_tag="VGY24_001685"
     CDS             19..939
                     /locus_tag="VGY24_001685"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ4284639.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            993..1241
                     /locus_tag="VGY24_001686"
     CDS             993..1241
                     /locus_tag="VGY24_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ4284640.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1366..2589)
                     /locus_tag="VGY24_001687"
     CDS             complement(1366..2589)
                     /locus_tag="VGY24_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4284641.1"
                     /translation="MTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYF
                     DTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKHRLG
                     SIETTSLMNFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANERYERIIIIV
                     MSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNRLESLFRK
                     PNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENY
                     ELVAKEYGKIFYFQNQVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIAKTTDK
                     NLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNIT
                     VKDNDPKGSIFEVKL"
BASE COUNT          921 a          484 c          373 g          852 t
ORIGIN      
        1 aaatgattag gagctaccat gtcaaatgaa tttattaact ttgaaaaaat ttctagagag
       61 tcttggaaaa cattacatca aaaggcaaaa gctctcctca cacaagagga acttaaaagt
      121 attactagct taaatgataa catcagtatt aacgatgtca ttgatatcta ccttccatta
      181 attaatctga tccaggttta caaaattgcg caagaaaatc tttctttttc taaaagcctt
      241 tttctaaaaa aagacattca attaagacct tttattattg gaatttctgg ttcagtagct
      301 gttggtaaat caacaactag tcgtctttta caacttttat tatcacgaac gcaccccaat
      361 agtcaagtcg aactggtaac gactgatggt tttttatatc ctaatcagtt tcttattgaa
      421 caaggactgt taaatcgtaa aggatttcca gaatcttata acatggaact tctcttagat
      481 tttctagatt ccatcaaaaa tggtcaaact gcttttgcac ccgtctattc tcatgatatt
      541 tacgatatta tcccaaatca aaaacaatca tttaataacc ctgacttcct tatcgtcgaa
      601 ggtattaatg tttttcaaaa tcaacaaaac aatcgtttat atatgagtga ttactttgat
      661 ttttctattt atattgatgc agacagcagt cacatcgaaa cctggtacat tgaacggttt
      721 ctaagcattt taaaattagc aaaacgtgac cctcataatt attatgccca atatgcacaa
      781 ttacctcgat ctgaggctat agcatttgcg cgcaacgttt ggaaaaccgt taatttagaa
      841 aatcttgaaa aattcattga acctacacgt aaccgtgctg aattaatttt acataaaagt
      901 gctgatcata aaattgatga aatttacctc aaaaagtgat aaagggttgc caaacgcata
      961 tattttctat ataataatgt agttagatta atttggaggt gaaaacattg gcaaatatta
     1021 aatcagctat taaacgcgct gaacttaacg ttaaagcaaa cgaaaaaaac tcagcacaaa
     1081 aatcagctat gcgtactgct atcaaagcat ttgaagctaa cccatctgaa gagcttttcc
     1141 gcgctgcttc ttcaagcatc gataaagctg aatcaaaagg tttgattcac aaaaacaaag
     1201 caagccgcga taaagcacgc cttgctgcaa aacttggcta ataaatatcc aatgaaaact
     1261 ccagctggag tttttttata cggtaaaaac tcaaaaaata caataaagcg ttgacagtta
     1321 aaaataactt taaaagggct gaccttattg gtcgcccttt ttagcttata gtttgacttc
     1381 aaaaatacta cctttaggat cattatcctt tactgtaata tttcctttta aagacatcac
     1441 gatttgctga gctaaagcca accccaagcc aaatccacct gtttgccgtg ttctagcttt
     1501 gtcaactcga taaaaacgat caaaaatctt tttcttttct tcatctgtta tccctggacc
     1561 attatcaata acagaaatta ataaattttt gtccgttgtt ttcgctataa tttcaataat
     1621 accattttta tctgtatatt taatagcatt gtcaaataaa atcgttatta attgttttag
     1681 taaagcctta tccattctta acgatctatt gacttggttc tgaaaataaa atatttttcc
     1741 atattcttta gcaactagtt cataattctc aaaaatagca ttaaaaaaat ctgtatctaa
     1801 atgagtccac tgtggattaa tgccatcatc tcgtcttgct aaatttaata aattagttgt
     1861 taagatgcgc atgttgcgaa cctcgtctaa actagaagcg agatgctcac tattttctaa
     1921 tatcgtttcg ttgggcttac gaaaaagcga ttccagacga ttctgtaaga ccgccaaagg
     1981 ggtccttagt tcatgactag cattttcaac aaacattttt tgtttttcat agctttctaa
     2041 aataggtttt ctgctccact tggctaaata aatacttgct aaaatagaaa ttagccaaaa
     2101 aacactcata actataataa taatgcgctc gtaacgttca ttagcgcggt ctaattgttc
     2161 cacatttact actgccatca tataggcaac tgcaggataa tttttgatat gaacccttac
     2221 ggttatcgta tggtattttt cttcttgtcc ataaaaattc attaaactgg tggtttcaat
     2281 actccccaaa cggtgtttat ctaaatgaaa attttgatag ttagaaaacg catcaaaagc
     2341 atttaaaatt gttccattag cactaaacaa aatgatatct gtattagctg caggcgttcc
     2401 taataactta gaactatctg aatcaggcag cgccttaata atgttatttt cagtatcaaa
     2461 gtaaaaagaa gatattctag ccatcgtacg attagcatag ctacttgcat tattactaac
     2521 agaaactaaa ctactgtcaa ctgacgaata aacgccaaac cgcataatct gtaagataat
     2581 aatagtcata atgacaaaaa tacctgtaaa aaccgcaaaa aaatgaaaaa
//
LOCUS       DAUCWU010000129         2569 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUCWU010000129 DAUCWU010000000
VERSION     DAUCWU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.129"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            180..2498
                     /gene="pcrA"
                     /locus_tag="VGY24_001688"
     CDS             180..2498
                     /gene="pcrA"
                     /locus_tag="VGY24_001688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ4284642.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT          823 a          485 c          541 g          720 t
ORIGIN      
        1 ggcgtgcaaa aaaataagga atgctttaag aaatcgctat tatttttaaa tatctgatcc
       61 taaactagaa aaaaggaatc tctttacttt tggggccttc tttgctattt ttcttggaaa
      121 catgatataa ttttaaggtt gataaaagag atgtcactga acgagattaa ggagaaagca
      181 tgaatccttt attaaatgga atgaatgatc ggcaggcaca agctgttcaa acaactgaag
      241 gccccttatt gattatggcg ggggctggtt ctggtaaaac aagggtactg acccatcgta
      301 ttgcctattt aattgatgaa aaatttgtca atccttggaa tattttagct attaccttta
      361 ctaataaggc tgcgcgtgaa atgaaagaaa gggccttggc cttaaatcca gctactaaag
      421 ataccttaat tgctaccttt cactccatgt gtgtacggat tctgcgtcgt gaggctgatc
      481 acattggtta caaccgaaac ttcacaattg tggatccagg tgagcaacga acgttgatga
      541 agcgcatttt aaaacaatta aatatagacc ctaaaaagtg gaatgaacgt tctattttag
      601 gaactatttc aaacgctaaa aatgatttgc ttgatgaaaa aggatacgag gcacaggctg
      661 ctgatatgta tagtcaaatc gtagcgaggt gttacaaagc ttatcaggaa gaattacgac
      721 gtagcgaagc acttgacttt gatgatttaa tcatgatgac gctacgcctg tttgacgcta
      781 atcctgacgt gttagcttat taccagcagc gttaccaata cattcatgtg gatgagtacc
      841 aagataccaa ccatgctcaa tatcagctaa ttaaattact agcttctcgc tttaaaaata
      901 tttgcgttgt tggagacgct gatcagtcta tctatggttg gcggggagct gacatgcaaa
      961 atattcttga ttttgaaaaa gattacccag atgctaaggt agtgctttta gaagaaaact
     1021 atcgctctac caagaaaatt ctgcaagcag ctaacgacgt gattaataat aaccgtaatc
     1081 gccgtcctaa gaaactttgg acacaaaatg cggatgggga acaactggtt tattacaggg
     1141 caaatgatga gcgagatgag gcagtatttg tggcctcaac cattagcaat atgagtcaag
     1201 agcttggaaa aaacttcaaa gatttcgctg ttttataccg taccaatgcc caatctcgga
     1261 ccattgaaga agcactctta aaatccaata ttccttacac catggttgga ggcaccaagt
     1321 tttacagccg taaagaaatt cgggatttga ttgcctattt aaccattgtt gctaaccctg
     1381 ctgataatat ttcttttgag cggattgtga atgagcctaa acgaggtgtt ggtccaggaa
     1441 cgcttgataa actgcgtcag tttgcctatg agagtgacca gtctttatta gaagcagctt
     1501 caaatctatt gatgtccccc ttgaaaggta aagcagcaca agctatcatg gacttggcca
     1561 atattctcgg tcagctacgt caagacctag atcagatgag tatcaccgac ttagcagaag
     1621 ctttgttaga aaaaacaggc tatttagata gcttacgcct gcaaaataca ttggaaagcc
     1681 aagcccgtat cgaaaatatc gaagaattct tatctgttac caaaaatttt gatgagtcaa
     1741 gtgcctctca agaagaagac gaaaccggtg ttgatcgctt gggacgtttt ttaaacgatt
     1801 tagcactgat tgctgataca gatgattccc aagcagaagc tgctgaagta accttaatga
     1861 ccctacatgc tgccaaaggc ttggagttcc ctgttgtttt cttaattggt atggaagaag
     1921 gtgtcttccc cttatcacgt gcctctgaag atccagatga attggaagaa gaaagacgtt
     1981 tagcttatgt cggaatcaca agagctgaag aagttctctt tatgaccaat gccaacacca
     2041 gaaccctctt tggtaaaagt agttataacc gtccaacacg cttcttgaaa gaaatttcag
     2101 aggaactact ctcttataaa ggattggcaa gaccagcaca atcttcattt ggtgtgcgtt
     2161 tttctactga aacacacaca cagtttggac aagggatgag cctatcagaa gccttacaag
     2221 ctcgaaaggc ccaggctcaa gtaagacaat cagcacagcc aatgcaggca catacgatac
     2281 catcagcttc aactagctca gttttaccgt ttggttctaa tagctctgta gaagaggtca
     2341 cttggcagat tggcgatatc gctcaccata aaaaatgggg agacgggact gtgcttgagg
     2401 tttcaggctc cggaaaaacg atggaattga aaatcaaatt tccagaagtt ggtttaaaga
     2461 aattactagc aagtgttgct cctattgaga aaaaataaga aaaaaatgag ctccctacta
     2521 tataagtgag taggaagctc atttttttag atttctgtat catataaaa
//
LOCUS       DAUCWU010000130         2480 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUCWU010000130 DAUCWU010000000
VERSION     DAUCWU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.130"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            1036..1173
                     /locus_tag="VGY24_001689"
     CDS             1036..1173
                     /locus_tag="VGY24_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEQ4284643.1"
                     /translation="MKKRLTITLSESVLGNLEKMAKEMGLSKSAMISVALEDYKKGQV
                     K"
     gene            1233..1865
                     /locus_tag="VGY24_001690"
     CDS             1233..1865
                     /locus_tag="VGY24_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEQ4284644.1"
                     /translation="MAKEKARYFTFLLYPESIPNDWELKLETLGVPIAVSPLHDRDKS
                     NVEGQKYKKSHYHVIYIAKNPVTADSVRKKIKLLLGEKSLAMVQVVLNVENTYLYLTH
                     ESKDAIAKKKHVYDKADIKLINNFDIDRYVTLDVEEKAELFNVVVSLIRAYTLQNIFD
                     LYDFIDENGETYGLTINLVNEVISGKTGFMKLLFDGAYQRSRRGEINRGK"
     gene            complement(2133..2441)
                     /locus_tag="VGY24_001691"
     CDS             complement(2133..2441)
                     /locus_tag="VGY24_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284645.1"
                     /translation="MKKKKIYSFLATSLIAISTSMTFSTSTVYAQSDSLGNSPADAII
                     DKIYRNLSPQEKRRIDQARANNAKCLAKAGISAVGAYATGGSASAASAFLTTLASCGW
                     "
BASE COUNT          792 a          358 c          510 g          820 t
ORIGIN      
        1 acagtgtttt tgtcttgttg ggattctcct aaaaccataa taaaaaagat cagtattatt
       61 taaaaatgct ggtctttttt taatgtcatt gcattcgatt ttttactcac tagtgttcgt
      121 atgattttat ttttagtcga gcgtagcgac aaattcagat gacatcatct gaataaggtt
      181 tcagccactc taataagccg tttaagcgat ttgtccgcac aaatccgttg cttgcctgaa
      241 tcagattgtg aaatgcctta aaacgcaaat atgagccttc tgagaggttt tagagataat
      301 ctaagcgatt tatgccgtga aagctccttg acgataagca gggacaaggt agacttagct
      361 ggggcggttt tgtcagaact ttgttcgacc gaccggcaaa gcaagtatcg tcgtccctgg
      421 tttcttgtcg tcaaggagac catggaataa agtaagcgtg catgatataa taaccaagtc
      481 gcattcacta aacagcgaaa gttgggaggt gaaactttat gctaaaagta tttcttgaac
      541 ttgttttgct accgctgaca gtcagtgtag ttgcagaact aattgcggaa tggttaatcc
      601 gtatgtggca aaacaaaggc gacaaataac ttaacttgag caccttgagc aagtgcaata
      661 aaaaatacct aggttgcccc ctaggtattt tctttatgct tatatttcct gaacttttaa
      721 gcaccttcat tgtatcacgt gtgagataat gtgtcaaaaa ttgggttggt tggaggaatt
      781 ttgagggggt ttttcgttag aaaaatccag aatatggggg gctactacga ccccccatat
      841 agtgccgagt gccaaattcg aaaaaaaagt gggttgaagc ttagagcctc aagcgtttga
      901 ggggtgttaa acctcggcga cttttcggcg actttttagc gactttttga taatttttta
      961 gaaatagtac gaagaaagtt gcatttaatg cgtagttttg ctaatatgaa cttataaaat
     1021 tttgagaggt gacttatgaa aaaaagatta acgataacgc tcagtgaatc tgtacttgga
     1081 aatcttgaaa aaatggcgaa agagatgggg ttatcaaaat ctgcaatgat ttcagttgcc
     1141 ttagaagatt acaagaaagg tcaagtaaaa taaaaaagcc gagctggcag gcgggctttt
     1201 agatatatta taacttgggg atattatact ttatggctaa agaaaaagca agatacttca
     1261 cttttttgct ctatccagaa tcaattccga atgattggga attgaagctt gaaactcttg
     1321 gagttccgat agcggttagt ccgcttcatg atagagacaa gagtaatgtt gagggacaga
     1381 aatataaaaa atctcactat catgtgattt atattgctaa aaacccagtt actgcagata
     1441 gtgttcgtaa gaaaattaag ttgttacttg gagaaaagag tttagcaatg gtgcaagttg
     1501 ttttaaacgt tgaaaataca tacttgtatt tgactcatga gagcaaagat gcaattgcta
     1561 agaaaaagca tgtttatgat aaggctgata taaagcttat caataacttc gatatcgacc
     1621 gctatgtgac gttagatgtg gaagaaaaag cagaactttt caacgtggta gtttctctta
     1681 ttcgtgcata tactcttcag aatattttcg atttgtatga ttttattgat gaaaatggag
     1741 agacttatgg attaactata aatttggtta acgaagttat ttcaggaaaa actggtttta
     1801 tgaaattgtt gtttgatgga gcttatcagc gttctagacg tggtgaaatt aataggggta
     1861 aataagtttt agttttgata tttaaaatag gcaagtagtt aaaattttat ataaagatat
     1921 atgtttatgg ggtgtaaaaa tgagtaaatt gttaagactt ttagttcaat cattgatttt
     1981 tttattatta ctttttgtta tagaaaaaat aaatttgaat gaactgttca gatatgtatc
     2041 tatatttttt gcagcactta ttgtgttcaa taaaaaatgg tttagtaaat aaaaaatagc
     2101 gtatatacgc tattttttta tttactaaac gttcaccaac cacaacttgc taaagtagtt
     2161 aagaatgcac ttgctgcgct cgctgaccct ccagtagcgt aagcaccaac tgcagaaatt
     2221 ccagctttag ctaaacattt agcattattt gctctggctt gatctattct tcttttttct
     2281 tgaggactta aatttctata tatcttatct attattgcgt cggcgggtga attgcctagg
     2341 ctgtctgatt gagcgtaaac ggttgaggtg ctgaaagtca tagaggtaga tatagcaatt
     2401 aaagaggttg ctaaaaaact ataaattttt ttctttttca taaaatatct cctgataatc
     2461 taaaggtatt gaaagtgtat
//
LOCUS       DAUCWU010000131         2420 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUCWU010000131 DAUCWU010000000
VERSION     DAUCWU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.131"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(292..492)
                     /locus_tag="VGY24_001692"
     CDS             complement(292..492)
                     /locus_tag="VGY24_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEQ4284646.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            complement(505..732)
                     /locus_tag="VGY24_001693"
     CDS             complement(505..732)
                     /locus_tag="VGY24_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ4284647.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1049..1735
                     /locus_tag="VGY24_001694"
                     /pseudo
     CDS             1049..1735
                     /locus_tag="VGY24_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1923..2168
                     /locus_tag="VGY24_001695"
     CDS             1923..2168
                     /locus_tag="VGY24_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ4284648.1"
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGA
                     GMATLGVGGVAGAFAGAHVGAIAGGLTCVGGMLFNGK"
     gene            2183..2365
                     /locus_tag="VGY24_001696"
     CDS             2183..2365
                     /locus_tag="VGY24_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEQ4284649.1"
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITG
                     GAAAFVCVASGIAAYQ"
BASE COUNT          785 a          415 c          416 g          804 t
ORIGIN      
        1 ttttaaagtc tttttcattt taatttcact cttttgtatt ttaaaaagct aagagtgata
       61 tctaaattca ttggcataat cactattttc ttttatcgat aaaaacaaat aatgacgctc
      121 ccaaagctac gatagataac atgtttaccg atttttccga aagggataat tgattgaaaa
      181 ttaaaaacac taaatatggt atagatttat caacattttt ttatgaatgg catcaacaat
      241 cattaaaacc attatagatg cattcataat aataaaaatc gtatagatca tttaatttgc
      301 tgtatatccc aaatatgctc caccaactgt aaatcctaca acacatccac ctgtcaatac
      361 tggatgtggc agtttacaaa atttcaatcc agaagctaag cctccaagac caccaattac
      421 agcatctcct ggagcgtttc ctccacttat actagccatg tcattaattg ttaattcctt
      481 cattgttgtc atcctttctt tcctctagaa tccgcctgta gctcctgtta caccagccca
      541 aagagtaata gctgttttag ctccaatata accacaggct acaccgcaaa ctggaaaacc
      601 tatagcagct ccagcactac ctgcagcgat aaccccactg acatttgcct gccaattatt
      661 cttaccaccc tcaaccttgg caagcttttc tatagtcatt tggtgaaatg tttccagttt
      721 ctttatctcc atatcgaata acttcttttc ataattctta aatttgtctc gttgcaacct
      781 cgactagtac agaatatcat aaagaacagt atcatggttt caaaatctta aaaggctgtt
      841 tttcatcatg aaatgtcttt ttatcctctc tacacttacg ctgtgacaat agttaattaa
      901 ttaagaagtt taaataatac cattcatgct tttataaata ccttttaaga tttttgatgg
      961 tagtgtaaaa tatcttgtgt aaacacacaa aaaggaataa atcctgtata gtagagttgc
     1021 gaaacttact agaaagagat ttattcccat gactcagttt accacagaat tacttaactt
     1081 cctagcacaa aaacaagata ttgatgaatt ttttcgctcc tctttagaaa tagctatgaa
     1141 tgacctcttg caagttgaac tatcagcttt tcttggttat gaaccttatg aaaaagaagg
     1201 ttacaacaca ggtaatagcc gtaatgggac ttattctcgt cagtttgaaa caaagtatgg
     1261 cttagtcaat ttaatcatcc caagagatcg aaacggcgag ttttcaccag ttttattacc
     1321 atcttatgct agacgagagg accacttgga agagatagtg atcaaactct atcagactgg
     1381 cgttacgaca cgcgaaatca gtgacattat taagcgcatg tatggtcacc acttgtttct
     1441 gagtggaagc ctcgttacaa aaaggttatg acatcacttg aaacaacgga aaatctccta
     1501 actttctatc aatttccaca ttacatttgg tctagtattt actctacaaa cttgattgaa
     1561 tcactcaata aagaaatcaa gcgacaaagc aagaaggagg gtggtttttc caaatgaaga
     1621 agccttagaa cgatgtcttg taagtatttt cgaagactat aatattaagt tcggagctcg
     1681 tattcataaa ggattcggag tatgttttga cacacttgat agcttatttg actaatattc
     1741 aaaaactcta ctcttgtgtt tacacaacat tattgacagt ctctaatatg ttaaaaagtt
     1801 tatttgagat gtttatacaa ccattcacga catagaaaaa aatttttata ataatatgct
     1861 atatttagtt tgtcaaagtt gcaacaagat gaatataaca ttttagaaag gtttttaaag
     1921 ttatgataaa atttgcagaa gaaatacaaa aagaagagtt gttccatatt attggaggtt
     1981 atagtgctac agattgtaaa aatcatttaa ttggtggtat tacttcaggt gctattgcag
     2041 gtggtgttgg agcaggtatg gctactttag gtgttggagg agttgcaggt gcgtttgcag
     2101 gtgcacatgt aggtgctatt gcgggtggat taacttgcgt tggtggtatg ttatttaatg
     2161 gaaaatgatt ttgaggtgaa aaatggataa ttttttagaa ttacagtttg aagaattagt
     2221 aaatatttct ggtggaaaag gaaatattgg atctgctatc ggagggtgct taggtgggat
     2281 gcttatagct gcagcaggag gtccaataac tggaggtgcg gcagcatttg tatgtgtagc
     2341 atctggaatt gcagcttatc aatgagatga ttatagttat aatactcggt ttaatttgtc
     2401 taatactttc ccaaattttt
//
LOCUS       DAUCWU010000132         2411 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUCWU010000132 DAUCWU010000000
VERSION     DAUCWU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.132"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1344
                     /gene="cls"
                     /locus_tag="VGY24_001697"
     CDS             <1..1344
                     /gene="cls"
                     /locus_tag="VGY24_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ4284650.1"
                     /translation="ISRMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVDLS
                     APYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIYNNTRVTYFPTGETFFDSLKE
                     QLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAK
                     RLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFG
                     HWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSP
                     LDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERGVDIRIIMPGVPD
                     KGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHF
                     ECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL"
     gene            1684..2022
                     /locus_tag="VGY24_001698"
     CDS             1684..2022
                     /locus_tag="VGY24_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284651.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT          769 a          366 c          446 g          830 t
ORIGIN      
        1 atttcaagaa tgacgtggtt aattttgatt atgattgctc cattactagg gtcactgttt
       61 ttaatttata caaaattgga ttggggttac agagggctaa aacagagaat aaatcatctt
      121 gtagatctgt ctgcacctta ccttagcgat gatgacgcta tattagaggt attaaaggat
      181 agcacatcaa ccacatatca tttagtacag tacttagaaa gaagtcgtgg caactttccg
      241 atttataata atacaagggt aacttacttc ccaacaggtg aaactttttt tgatagttta
      301 aaagaacagt tattcttagc taaaaagtac atttttcttg aattttttat tattgcagaa
      361 ggccaaatgt ggggagaaat ccttagtatt ctagaaaaaa aagtcagtga aggtgtagaa
      421 gttagggtgt tgtttgatgg catgaacgaa ctatcaacgc tatcatcaga ttacgccaag
      481 agattagaac aaatagggat taaagctaaa tcatttttac cgatttcacc ctttatctct
      541 acctattaca attatcgaga tcaccgaaaa attgtcgtta ttgatgggga ggtatcattc
      601 actggaggta ttaatctagc agatgagtac attaatgaag tagagcgttt tggccactgg
      661 aaagatgctg gtttaatgct tgagggtgaa gcaacagaca gctttttaat tttgttttta
      721 caaatgtggt ctatcacaga aaaagaactg attattgatc cttatctttc agatcattct
      781 ttaaaacttc cttcagatgg ctatgttatt ccctacggtg attccccgct tgatactgat
      841 aaaataggta aaaatgttta tatagacatt ttaaatcatg ctaaagagta cgtttatatc
      901 atgacacctt accttatttt agatagcgag atggaacacg ctttacggtt tgcatcagaa
      961 cgtggagtag atatccgaat tattatgcca ggagtgccag ataagggggt accatatgcc
     1021 ttagctaaaa cctattataa agctttgatg tcttctggag taaaaattta tgagtatcaa
     1081 ccagggttcg ttcattcaaa ggtatttatc tctgataata caaaagcagt tgtcggaaca
     1141 attaacttag actatcgtag cctttatcat cattttgaat gcgcgactta tttatatcgt
     1201 gtgtcagtta ttgctgatat tgtgaatgac tttaatgaag ctcaaaagca atcactattg
     1261 atgacatcag atcatttgac gcaacgtcct tggtatcaaa aattgatagg attattagta
     1321 cgaacaattg cccctctctt gtaattttcg taaaatatgt tataatatct gtatgcgatg
     1381 agtcgattgt gactgcaaag tcacttttgc gcaggggagg tcattaaggc gggaagccga
     1441 ctttgataat ctgtgtgaac cattttatca caatcttatt tattaagaat gccccttgga
     1501 ttgagatatt ctcaatcctt tttttaattt tattatctta gttaaaattc agaatgtatc
     1561 agtgattact gtgtttcaaa ataagaaagt agtttttcgg gtatgagtgc tatgacttga
     1621 caaatgctaa taacatttgc ataatgtaag tagagatgaa aacttatgaa aagagatgat
     1681 cttatgcaaa ttattaagcg tgatggccac gttgctgatt ttgatccaga taaaatttat
     1741 caagcaatta ttaaagcagc aaggacggtc tatgttattg atgacacgtg gcgacaaaac
     1801 ttaacacatg tcactaaaaa agtagttatt gatttagaag aagcacatgt agaacgtcct
     1861 actatcaata tggtccaatc ccttgtggag aatcgtttga tggatgctgg atatattacc
     1921 attgcagaac attacatttc ttatcgtctt cagcgagatt tagagcgcaa tggctatggt
     1981 gataagattg tggttcactt gcgttttgaa caaataaaat aaaaacgctt ctaatggcat
     2041 tttagtgtct ttgaggggat aaggtttaaa tacctaacag ataaaaatct gttaggtatt
     2101 tttttgctac tattaggtgt attctatatt acatagaact taagcattca agctttaatt
     2161 taactttctg tttatttttt gctacatttt gggaaactcc cctcctttct tagttgaggt
     2221 gaataaactt ttatttgata aatcatatcc ttagtcttaa tgaaacagtc taagataata
     2281 ctacttcgaa gtgacagcta gtagtttcca atttttaagt agtctaaaat tatgtttttt
     2341 taatttcaaa accatgcttt cgtttgttat aaaaaccgat cttccatagt tagatttttt
     2401 atccaggcat t
//
LOCUS       DAUCWU010000133         2340 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUCWU010000133 DAUCWU010000000
VERSION     DAUCWU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.133"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            66..917
                     /locus_tag="VGY24_001699"
     CDS             66..917
                     /locus_tag="VGY24_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ4284652.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            910..1752
                     /locus_tag="VGY24_001700"
     CDS             910..1752
                     /locus_tag="VGY24_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ4284653.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            1730..2317
                     /locus_tag="VGY24_001701"
     CDS             1730..2317
                     /locus_tag="VGY24_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ4284654.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
BASE COUNT          787 a          362 c          447 g          744 t
ORIGIN      
        1 gcactaccag ttttgcttta tttagtaagg agtgttagaa taaagaggtg agaaactagt
       61 ttagaatgga aaaatacatg ggaactataa aaattgttac agattcatca ataactattg
      121 aaccagaatt aattaaagct ttagatatta ctgtagtgcc tttatctgtc atgattgata
      181 gcaaattata ttctgataat gatttaaaag aagaaggtca ttttttgagc cttatgaagg
      241 ctagcaaatc ccttcccaaa accagtcagc caccggttgg tctctttgct gaaacttacg
      301 aaaatttggt caaaaaagga gtaactgaca ttgttgctat tcacctctca ccagctttat
      361 caggtactat tgaagcttcc cgtcaggggg ctgaaatcgc tgaggcacct gttacagttt
      421 tagattcagg atttactgat caagccatga aatttcaggt cgttgaggca gcaaagatgg
      481 caaaagcggg tgctagtttg aatgagattt tggctgctgt tcaagctatc aaatcaaaaa
      541 cagagctcta cattggtgta tctaccttag aaaatttagt aaaaggaggt cgtattggtc
      601 gtgtgacggg tgttttgagc tcgctactta atgtcaaagt tgttatggca cttaaaaatg
      661 atgagttaaa aacgcttgtc aaaggtcgag gtaataaaac atttacgaaa tggttagata
      721 gttacctcgc taaaaatagt catcgcccga ttgcagagat agctatttct tatgctggtg
      781 aagctagcct ggcattaacc ttaaaagaaa gaatcgcagc ttattataac cactctattt
      841 cggttttaga aacaggctct atcatccaaa cacatacagg agaaggagct tttgcggtta
      901 tggttcgtta tgaataatcg tcatttattt agtgggatat ttttctttgt tattagttta
      961 tgtctggcct ttttattgtt aaatattatt atccctaagt caaattcacg tttgaaaaag
     1021 agtgattttc tgaaaaaaga acaagtagct atccaatatg ttgctatagg agattcattg
     1081 acagaaggag taggtgatct aactcatcaa ggtggttttg ttcctttgtt aacgaatgat
     1141 ctcagtgaat attttaaggc taatgttaat catcaaaatt acggcgtatc tggtgatacc
     1201 agtcaacaaa ttcttgatag gatgataaaa caaaagcaga tacagttatc tttaaaaaaa
     1261 gcagatataa tgacgttaac cgttggtggt aatgatgtta tggcagttat tcggaagaat
     1321 ttagcggatt tgcaagtttc tagttttaga aagccagctc gtcagtatca aaaacgatta
     1381 agacagatta tcgagttagc cagaaaagat aataaagatc ttcctatttt tattttaggc
     1441 atctataatc cgttttattt gaattttcca gaactaactg atatgcaaaa agtgattgat
     1501 gactggaata ccaaaactaa ggaggttgtt ggagaatacg atcgtgtgta ctttgtgcca
     1561 ataaatgacc tcttgtataa agggataaat ggacaagaag gaattgtcca ttcttcagga
     1621 gatcaaacta caattgtcaa tgatgccttg tttactgggg accattttca cccaaataat
     1681 actggctatc aaatcatgtc aaatgcagta atggagaaaa taaaaaagca tgaaaaaaaa
     1741 atcaaacctt aattggtgga agtggagctt cttatgtctg cttgctttta ataccgcctt
     1801 tttgatggtt attgcaagtc gtctcattca agtaagagaa cctgagtcag aactgattgc
     1861 taaaaaacca gttaaaaata tcaaaattgg tacatttgtc acaaccagag aacagcttaa
     1921 cgaaactgta gcaagttatc ttaaagatta tcagactaaa aaaatgtctt ataaatttta
     1981 tgcgacatct tcttctattt tgtttgaagg aacttatcaa ttattagggt atgaagtccc
     2041 tttatatatc tattttcaac ctcatcgtct agaaaatgga gctgtccaac tacaggtgat
     2101 ctctttttca gtgggaactc tgccactccc agaaaaagat gttttgcagt atttgaaatc
     2161 aagctacaaa ttgccaagct ttgttaaagt gatgccaaat caatcagcta tcgttgttaa
     2221 tttacaagat atccaaaatg atgctaaggt ttatttaaag gccaagaaaa ttgatttatt
     2281 taatgatgaa atcagtttta acatctataa gaaataagct caaataacgt taaatacttg
//
LOCUS       DAUCWU010000134         2291 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUCWU010000134 DAUCWU010000000
VERSION     DAUCWU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.134"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1562)
                     /locus_tag="VGY24_001702"
     CDS             complement(<1..1562)
                     /locus_tag="VGY24_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ4284655.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTTQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVIATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVTAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLIIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGL"
     gene            complement(1559..2248)
                     /locus_tag="VGY24_001703"
     CDS             complement(1559..2248)
                     /locus_tag="VGY24_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis protein F domain protein"
                     /protein_id="HEQ4284656.1"
                     /translation="MLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDMLVKP
                     ADSILSAFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQIDFQ
                     AMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYIAWGN
                     SYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPAKAMK
                     YIFLRLMEKLK"
BASE COUNT          799 a          409 c          321 g          762 t
ORIGIN      
        1 atgagtcccg cagttgtgct atttttgatt gccttgatat taggtaaaac atcgtggcta
       61 acagtaacat ttgatactct atcaggatct gacaagcttt cacttaaaag ataaataacc
      121 gtttggtcag caatattatt ttttctgtct ttattaatat cttctgctaa tttttggtac
      181 ttctgagcga tactcgctat tgtttcttgg ctataatttg tcggtttttc cattattttc
      241 ttggtcacct gtcttgtcca aacatatgct aaagatttgt acctagcatt tgttgaaaaa
      301 cctaaccagt ttatgtcatt atcattattt aaagccgaaa taatcggaag accagataat
      361 attttacctt ctttttcata agaaaatggt attaacatac tacaagatac aataattaaa
      421 cctaatacag cacttgcccg tttgacaggg ctcataaata atttccctgc taaaactcgg
      481 ccatgtagat accaagccac cacaatacaa atagcaagtc ctacaaccat ttctacaatc
      541 accgattttt taacaaagct tgtcactaaa cctaattctt gtaaccaaac aaaatcagtt
      601 actaataatg gctcgcttct cattgattct tttaaatcat taacaacaga aataattgtt
      661 cccagaataa gcaaaaagaa ggtagttggc caatatctat tcgttatcac gtatgctaaa
      721 agagcaacta gtgtaatagc tactatctga aaaagagtag ctccagggaa aacataatat
      781 cctagcaatg cttcatcacc ttttactcca tactggaacg tatagttaaa aactaatgct
      841 aaaaacaaac tagtagtagc tgctactgaa aatgaaggtt tatttgactt aaggtgtttt
      901 agagcattta aaaccacgta tatgattgtt gcaaatatga caagtaaaac tatcaacagt
      961 ccacttattt ttaacagtgc aatttggcta ttacctgctg ttactaattc agaaaatcga
     1021 gaacttatta gttttaacag atgtatgtca ttaattggca caactgctaa aatcagagct
     1081 acagaaaaag tattaccttg atagcctaat agcccaaatc tatctttttc tattgcaaaa
     1141 tatgctacca atatttttat taaagtggct atcactaaaa taatgagtaa agaaggtaga
     1201 gcttgaaatt gataaaattg atttttagtt aatgaccaga tatcaaaccg tttatcattc
     1261 aggtgctgtg ttatttgtac ccaatagctg acaattagat aagatatcac aataaaacta
     1321 gttttcaaaa acaactgtcg actaacttgg cgactcaaat aataagataa tccaatcagt
     1381 gatataatat atcccacaaa agaaacattt agtaaatgag tataatagtt taccggttgt
     1441 gtcgtggagc ttactagttc tttcgccaaa ctgtaaaaac tgaaataaaa accgaaaaca
     1501 agaaggataa tatggtgact aatatttaac caattggttt ttaaaaatgt gtctttaatc
     1561 atttaagttt ctccattaaa cgcaagaata tatacttcat tgctttagcg ggaccaacga
     1621 taatatattt taatgctcct ttgataccgc ccatttgatt aaagttaacg taggtatgag
     1681 ctccttcatc ttcacgtaaa tttaacagtt tattgtcaat aaaaggtgtt aactcatagg
     1741 gatttttaac aatcctaaaa tcataagaat tcccccacgc tatatagaca agtaaccgct
     1801 caatcgcatg taatatagag ttttgtggta aaggttcact tggaatatca ttttgagtta
     1861 attctaaatc aaaaagtgat tttagagcat cgtatttaaa ccatacaaat gttccataac
     1921 tcataacaaa tgtatccata gcttgaaaat caatttgttt tttaacatcc atttttctcc
     1981 acaaagacat catttcttga gcgattaaat gctcattcca agcattgaca atcttattaa
     2041 atctaaaaaa acttggaata tctgcgatta taataccaat atcatctgtc tcaaaagcgc
     2101 taagaatact atctgctggt ttcacgagca tatcaatcaa ttcttttcgc caagattctc
     2161 ctgcccaaaa atcagcttct tttgattttt tcgtatggaa atgaccaata taatcatatt
     2221 gagaaagttt atcttttagt aataacattg gataaatatc tcttcctcta tttcccgtaa
     2281 cgcgtatatc a
//
LOCUS       DAUCWU010000135         2284 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUCWU010000135 DAUCWU010000000
VERSION     DAUCWU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.135"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(225..740)
                     /locus_tag="VGY24_001704"
     CDS             complement(225..740)
                     /locus_tag="VGY24_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284657.1"
                     /translation="MYADSNLKFEEISSAAKLILSLYRQVTWAVDSRANFMMFESKEN
                     YGSTSESAYLYLSTFAPERVEEKFNNRVSNVMDSKLMLLIIYDACVRLKVYPEYGEIY
                     HKIIYNYYISEKKITDKACMRSVSLERTVYYQRKKEAIALVGVIIWGYTLPTAISQLE
                     DGRSIEEIMNI"
     gene            935..1336
                     /locus_tag="VGY24_001705"
     CDS             935..1336
                     /locus_tag="VGY24_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284658.1"
                     /translation="MAFADRLKEFREKEKLSQADFAKMIGISTRTLVHYEDGERYPRD
                     VEVYKKIAEVMDCDYNYLLEESDEFLNRVYNMGGKRELEKARALTEGLSSLFAGGEIS
                     DEDKDAAFEAITRAYWEAKRENKKYGRKKKD"
     gene            1345..1893
                     /locus_tag="VGY24_001706"
     CDS             1345..1893
                     /locus_tag="VGY24_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ4284659.1"
                     /translation="MSMNRYIYDKVNRLIKKYKTRDPVELIEALNINLVYLPETKILL
                     GMYHYIQRNRFIFISSNTNFNRKTILAHELGHDQLHRDYCMSGGAFHDQTVLNPTNKF
                     ETEANIFAAHLLISDEDVLNNINDQVCDYEIAGKLGVDINLLNLKISELAKMGKFNKP
                     INVNTPQGDFLKNYRPEHDDYY"
     gene            1902..>2284
                     /locus_tag="VGY24_001707"
     CDS             1902..>2284
                     /locus_tag="VGY24_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284660.1"
                     /translation="MGIFIAGYILLILSGIVFYYLITTYSVKISAFLIDFIVVGLGFY
                     ISIKDKLDNKLAIPLSITVVIIYGILLVLINRKLPKVSRFLNYIIAFIGSSVALWLAL
                     DFITNTLAAFKIIGQTYHQLPITKN"
BASE COUNT          832 a          342 c          350 g          760 t
ORIGIN      
        1 aaattcacac aaggagggct tctaattctt tgcatacatt ctcctgggca taaaaaaaga
       61 ccgaaagaaa ataaattctt tcagtcaata aatgccaata ttaaatttag gtgcagatca
      121 cccatcatgg atattatata tgcacttaag tacacataaa attcgtatat ggtacttttt
      181 tcattcggaa atagtacgca ataagttcgt aataaaataa tttcttagat gttcattatt
      241 tcttcaatgc tacgaccgtc ttcaagctgg ctaatagcag tcggtaaagt atatccccaa
      301 attattacgc caaccaatgc aattgcttcc ttttttcttt ggtaataaac agttctctct
      361 aaagatacac ttctcataca agctttatca gtaatctttt tttcagaaat gtagtagtta
      421 taaataatct tatgatagat ttctccatat tctggataaa cttttagcct aacacaggca
      481 tcataaatta ttaaaagcat aagtttacta tccattacat tggaaactct attattaaac
      541 ttttcttcaa ctctttctgg agcaaatgta gaaagataca agtaagcaga ttctgaagtt
      601 gacccatagt tttctttact ctcaaacatc ataaaatttg ccctgctatc tactgcccag
      661 gtaacttgtc tatataaact taaaataagt tttgcagctg aactaatttc ttcaaatttc
      721 aagttgctat ctgcatacaa gttcattata ttctttaaag ttttacttat tctcaatcta
      781 atcaccccaa tacaacttat ttgcaaactt gaatgtttgt gctataatat gaatataagt
      841 tcagtttgta ttatatctca aatcgaaaaa taattcaagc actattgttc gtattaaaag
      901 ttataaatgc acattagtac aggaggttaa agttatggct tttgcggata gattaaagga
      961 atttcgtgaa aaagaaaagt taagtcaagc agattttgca aaaatgatag gaataagtac
     1021 aagaactctt gtacattatg aagacggaga aagataccca agagatgtcg aggtttacaa
     1081 aaaaatagct gaggttatgg actgtgatta caattatctt ctagaagaaa gtgacgagtt
     1141 tttaaatcga gtttataaca tgggtggcaa aagagaatta gaaaaagcta gagcattaac
     1201 agaaggtcta agctctttat ttgcaggtgg agaaatttct gacgaagata aagatgctgc
     1261 ttttgaagct attactcgtg cttattggga ggctaaaaga gaaaacaaga aatacggtcg
     1321 taagaaaaaa gattaggtga ttttatgagc atgaatcgct atatatatga taaggttaat
     1381 cgacttataa aaaaatataa aacacgagat cctgtcgaat taatagaagc tttgaacatt
     1441 aacctggtat atctacctga aacaaaaata cttttaggaa tgtaccatta tattcaaagg
     1501 aatagattta tttttatcag tagtaacaca aattttaata gaaagactat attagcacac
     1561 gaattaggtc acgatcaact tcacagagat tattgtatga gtggcggagc ttttcatgac
     1621 caaacagtgc taaacccaac aaataaattt gaaactgaag caaatatttt tgcagctcat
     1681 ttattaatta gtgatgaaga tgtcctaaat aatattaatg atcaagtatg tgattatgaa
     1741 atcgctggaa agttaggtgt agatataaat ttactaaatc taaaaatttc tgagttagca
     1801 aaaatgggca aatttaataa accaattaat gttaatacac ctcagggaga ttttctcaaa
     1861 aactatcgcc ctgaacatga tgattattat taggagactc tatgggaatt tttattgcag
     1921 gatatatttt attaatcctt tctggaatag ttttctacta tctcatcact acctactctg
     1981 taaaaatctc agcattttta attgatttta tagttgtagg tttaggattt tatatatcta
     2041 taaaagataa actagataac aaattagcta ttcccctttc aattacagtg gtaataattt
     2101 atggaatatt gctagtttta ataaatcgaa agctaccaaa ggttagcagg tttttaaact
     2161 atataattgc ttttataggc tcatcagttg ccctatggtt ggcacttgat tttataacaa
     2221 acactctagc cgcttttaaa ataataggtc aaacttatca tcaattacca ataacaaaaa
     2281 atat
//
LOCUS       DAUCWU010000136         2252 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUCWU010000136 DAUCWU010000000
VERSION     DAUCWU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.136"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..286
                     /locus_tag="VGY24_001708"
     CDS             <1..286
                     /locus_tag="VGY24_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEQ4284661.1"
                     /translation="ANYYIVEPQVDKVIPKLKKYIYYIHCKNYVNNDLGIHYVELGKG
                     IIDYKTIIHEIHEHFIDLIFSLEVHEKEIDKKAEVVRKSYLTLNEYINGG"
     gene            288..1022
                     /locus_tag="VGY24_001709"
     CDS             288..1022
                     /locus_tag="VGY24_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ4284662.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIA
                     SKVVSVAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            1015..2001
                     /locus_tag="VGY24_001710"
     CDS             1015..2001
                     /locus_tag="VGY24_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ4284663.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFIIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKIFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            2011..>2252
                     /locus_tag="VGY24_001711"
     CDS             2011..>2252
                     /locus_tag="VGY24_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ4284664.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEP"
BASE COUNT          839 a          273 c          442 g          698 t
ORIGIN      
        1 tgcaaattat tacatagttg aacctcaggt agacaaggtt attccaaaat tgaaaaaata
       61 tatttactat attcactgta agaactatgt taacaatgat ttaggaatac attatgtaga
      121 actaggtaaa ggaattatcg attataaaac aatcattcat gaaatccatg aacattttat
      181 agacttaatt tttagtttag aagtacatga aaaagaaata gacaaaaagg cagaagtagt
      241 gaggaagtct tatctgacac taaatgagta tattaatgga ggctaaaatg aatttgagta
      301 tatttggttt aaagaatatt ccttatttga aagaagggga tagtatagaa aagcttattg
      361 aagaaagtat taaaacttcg gaatttttta tcgaagacaa tgatgtgctt tgcatagctt
      421 ctaaagttgt atctgtcgca gaaggccagg tgatgtcttt aaacgagata caagtgagtg
      481 atgtagcaaa agaaatccac aggaatattc ctcgtaaaga tcctagaata attgaaatta
      541 tgctaaattt agtcaataga gatctgtcac gtttagatat caaaaaaaat tacattggtt
      601 gtcgcttgga aaatggttta aagttgacaa gtggtggaat cgatagaaaa tcagtagatg
      661 aagtatttct tttacctaat aatccggatg cttctgcaaa aagaatttcg gaatatctta
      721 aaaaatcact tggaaaaaat gttgcagttg ttataacaga tagtgatggt agagaggata
      781 aaagaggtgc tactcaagta gcaattggta tttatggtat tcatcctttg agaaaaacag
      841 aagtaataga ttcacaagga gagacaataa aattccaaga agagactttg tgtgatatga
      901 ttgcagcttg tgctggctta gtaatggggc aacgtggaac tggaattcca gctgtcctca
      961 ttaagggctt ggattataaa tggtctgaaa atactagtat taaggaagcg ttaaatgagt
     1021 agaaatcagg actgtaagaa tattctaatc attggttttg gaaaaattgg gaaaattaaa
     1081 gcgtacaagt ggctatcacg cggatatgag gtatatgtca aagatttgaa attctccagc
     1141 atgaaatacc ttaatacaaa tagtgatgtg ttggatgatt tatcacgaca atattttatt
     1201 attgagattt gtactccgac aaatcaccac cttacgctat tgaaaaccgt tataacaagg
     1261 tatgtctatt cttatattag cattgaaaaa ccactgtgta acaggttaga agatttagaa
     1321 gaaatgaagt cgctagtaga aagtcaccca catctagcaa aaaaaatttt tgccagtgaa
     1381 caatacttct tttcgaaaat tttggaaaaa ctgttacaag tagattgttt ctctctagat
     1441 aaaatcaagg aaataacagt taatttttct aaagatagga ttgaggataa cgagaatggg
     1501 agatttttag ataaagagat attgggctat ggaatagaga ttcctcatat cattgctgtc
     1561 atttcttatt taggaatttc attagaagaa ttcaaaaaag gaatctttgt gaatgctatt
     1621 tatataaaag acttagagaa acacgattat tcaatagaaa tattatcttg tattaatcaa
     1681 acagtgatta aaattattag taatttggga agttttgcaa taagagaggg caagattgaa
     1741 ggaggtgatg ctaaaacagt aagaagagtt accattgatg acaaaacaat actatttgat
     1801 ccacatccta aactcgagag gtataagagt gaattggtga ctgggacaaa tacgtttcaa
     1861 atacacgatg atatgatgga cacgtatatc agtttgttag aaggaaatgc tattcctgaa
     1921 gattgctata tcaataatgc aattgagata acaaaattat taattaactt atattcagaa
     1981 tgtgaaaaaa tttatttatg aggtgtaact atggatttaa aaattacaaa cggcttttat
     2041 gacccaagtc atttatcata cgaagtagta gaacgcaaag gcttaggtca ccctgatacc
     2101 ttagcagatg gaattgctga gcaaattgaa attgattact cactttattg tttggacaag
     2161 tttggtgtta ttccacatca taattttgat aagattatta tccgaggggg acattcagtt
     2221 caggattttg gtggaagtga ctttattgaa cc
//
LOCUS       DAUCWU010000137         2227 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUCWU010000137 DAUCWU010000000
VERSION     DAUCWU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.137"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1175
                     /gene="hasA"
                     /locus_tag="VGY24_001712"
     CDS             <1..1175
                     /gene="hasA"
                     /locus_tag="VGY24_001712"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HEQ4284665.1"
                     /translation="TSTVGIYGVILITYLVIKLGLSFLYEPFKGNPHDYKVAAVIPSY
                     NEDAESLLETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHR
                     SLVNKGKRHAQAWAFERSDADVFLTVDSDTYIYPNALEELLKSFNDETVYAATGHLNA
                     RNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPNLERYKNQ
                     TFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFRE
                     SIISVKKILSNPIVALWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIV
                     ALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            1211..>2227
                     /gene="hasB"
                     /locus_tag="VGY24_001713"
     CDS             1211..>2227
                     /gene="hasB"
                     /locus_tag="VGY24_001713"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEQ4284666.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLNSHMVIQGISYDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEEQESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILK"
BASE COUNT          758 a          358 c          376 g          735 t
ORIGIN      
        1 gaacatcaac tgtaggaatt tatggagtaa tattaataac ctatctagtt attaaacttg
       61 gattatcttt cctttatgag ccatttaaag gaaatccaca tgactataaa gttgctgctg
      121 taattccttc ttataatgaa gatgccgagt cattattaga aactcttaaa agtgtgttag
      181 cacagaccta tccgttatca gaaatttata ttgttgatga tgggagttca aacacagatg
      241 caatacaatt aattgaagag tatgtaaata gagaagtgga tatttgtcga aacgttatcg
      301 ttcaccgttc ccttgtcaat aaaggaaaac gccatgctca agcgtgggca tttgaaagat
      361 ctgacgctga cgttttttta accgtagatt cagatactta tatctatcca aatgccttag
      421 aagaactcct aaaaagcttc aatgatgaga cagtttatgc tgcaacagga catttgaatg
      481 ctagaaacag acaaactaat ctattaacgc gacttacaga tatccgttac gataatgcct
      541 ttggggtgga gcgtgctgct caatcattaa caggtaatat tttagtttgc tcaggaccat
      601 tgagtattta tcgacgtgaa gtgattattc ctaacttaga gcgctataaa aatcaaacat
      661 tcctaggttt acctgttagc attggggatg atcgatgttt aacaaattat gctattgatt
      721 taggacgcac tgtctaccaa tcaacagcta gatgtgatac tgatgtacct ttccaattaa
      781 aaagttattt aaagcaacaa aatcgatgga ataaatcttt ttttagagaa tctattattt
      841 ctgttaaaaa aattctttct aatcccatcg ttgccttatg gactattttc gaagtcgtta
      901 tgtttatgat gttgattgtc gcaattggga atcttttgtt taatcaagct attcaattag
      961 accttattaa actttttgcc tttttatcca tcatctttat cgttgcttta tgtcgtaatg
     1021 ttcattatat gatcaaacat cctgctagtt ttttgttatc tcctctgtat ggaatattac
     1081 acttgtttgt cttacagccc ctaaaacttt attctttatg caccattaaa aatacggaat
     1141 ggggaacacg taaaaaggtc actattttta aataatatgt gcatcgagta gttagagaag
     1201 gagtaatttt atgaaaatag cagttgctgg atcaggatat gttggattat cactaggagt
     1261 tcttttatca cttcaaaacg aagtcactat tgttgatatt cttccctcta aagttgataa
     1321 gattaataat ggcttatcac caattcaaga tgaatatatt gaatattact taaaaagtaa
     1381 gcaattatct attaaagcaa ctttagatag caaagcagct tataaagaag cggaactggt
     1441 cattattgcc acacctacaa attacaacag tagaattaat tattttgata cacagcatgt
     1501 tgaaacagtt atcaaagagg tactaagcgt taatagccat gcaactctta tcatcaaatc
     1561 aacaattcca ataggtttca ttactgaaat gagacagaaa ttccaaactg atcgtattat
     1621 cttcagccct gaatttttaa gagaatctaa agctttatat gacaacttat atccaagccg
     1681 aattattgtt tcttgtgaag aaaacgattc tccaaaagta aaggcagacg cagaaaaatt
     1741 tgcactttta ttaaagtctg cagctaaaaa aaataatgta ccagtactta ttatgggagc
     1801 ttcagaagct gaagcagtaa aactatttgc caatacttat ttagcgttaa gggtagctta
     1861 ttttaatgag ttagacactt acgcagaatc gagaaaatta aatagtcaca tggttattca
     1921 aggaatttct tatgatgatc gaataggaat gcattataat aacccatcat ttggttatgg
     1981 aggttattgt ctacctaaag atacgaagca attattggca aattacaata atattcccca
     2041 aacgctaatt gaagctatcg tttcatcaaa taatgtgcgc aagtcctata ttgctaagca
     2101 aattatcaac gtcttagaag agcaggagtc cccagtaaaa gtagtcgggg tttaccgttt
     2161 aattatgaaa agtaactcag ataattttag agaaagtgct atcaaagatg ttattgacat
     2221 tcttaaa
//
LOCUS       DAUCWU010000138         2138 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUCWU010000138 DAUCWU010000000
VERSION     DAUCWU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.139"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..295
                     /locus_tag="VGY24_001714"
     CDS             <1..295
                     /locus_tag="VGY24_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ4284667.1"
                     /translation="NINANLVKEAEISEFEKDGESVQVANFALVKKYGKGKEYTNCSV
                     YGEKVEIVKEFEKGDLIHVFGYFKENKKGDKVYKNFIVKSLNKIENKKENEEE"
     gene            297..512
                     /locus_tag="VGY24_001715"
     CDS             297..512
                     /locus_tag="VGY24_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEQ4284668.1"
                     /translation="MEFFTAGVGVLKTLVTAIGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGIKLIPLLANVLN"
     gene            523..1386
                     /locus_tag="VGY24_001716"
     CDS             523..1386
                     /locus_tag="VGY24_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284669.1"
                     /translation="MFGIFDKLTEFFKDMLLGGIKANLESMFLDINDKVGVIATDVGK
                     TPMGWNGEVYNFIKNINDNVIVPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMFIAVYLASHAFEFSMAVFDVAQNLVNKAAGVITTSATVSGDQIVAMVDTLKEKE
                     IGELLMILVETSLVRIAIQCISLTITLIVYGRMFEIYVYSSVSSIPFATMGNKEWGQI
                     GTNYIKGLFALGLQGLFLMVCLGIYTVLIRTVQITDIHASLFSILGYALLLGLMMFKS
                     GTVAKSIMNTH"
     gene            1396..1686
                     /locus_tag="VGY24_001717"
     CDS             1396..1686
                     /locus_tag="VGY24_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284670.1"
                     /translation="MLKKKNCALLGLVILLGGTYEILKEKKRNEEIRNLKRRINRLGR
                     CHNEFVMYQGEYNDRNEERQEELEERISYLEEETISNYDHILELSKEEVEGD"
     gene            1688..2098
                     /locus_tag="VGY24_001718"
     CDS             1688..2098
                     /locus_tag="VGY24_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEQ4284671.1"
                     /translation="MAYVPIPKDLDKIKTKVAFNLTKRQLIGFSVAGVIGIPTYLFMK
                     KYLPNDVSIIVMLIVTLPIFFITLYEKDTLTFEKYFKFFYLHKFYQPTKRIRKEAYLE
                     AKKKANQRLKSKRKTIKKRPKGSKKAKNKERSNK"
BASE COUNT          835 a          255 c          418 g          630 t
ORIGIN      
        1 aaatattaat gcaaatttag ttaaggaagc tgaaatttca gaatttgaaa aagatggaga
       61 aagtgttcaa gtagcaaatt ttgctcttgt aaaaaaatat ggaaagggca aagaatatac
      121 aaattgctca gtatatggag aaaaggtaga aattgtaaaa gaatttgaaa aaggagatct
      181 aatccatgtc tttggatatt tcaaagaaaa caaaaaagga gataaggtct acaaaaactt
      241 tatagttaaa tcactaaaca aaatagaaaa taagaaagaa aacgaggagg aataatatgg
      301 aattttttac agctggagtt ggagttttaa agacacttgt aactgcaatt ggtgcaggtt
      361 taggagcatg gggagttatt aacttaatgg aaggttacgg taatgataac cctggtgcta
      421 aatctcaagg tattaagcag cttatggctg gtggaggaat tgtactaatc ggtattaaat
      481 taattccact acttgcaaat gttttaaatt aggagaaagt ctatgtttgg tatctttgac
      541 aagctaactg aattttttaa ggatatgcta ctcggaggta tcaaagcaaa tcttgagtcc
      601 atgttcttag atataaatga caaggttgga gttattgcaa ctgatgttgg gaagacacca
      661 atgggttgga atggagaagt atataacttc ataaaaaaca ttaatgataa tgtgattgtt
      721 ccaatagcag gtcttatcat aacagcagtt ttatgcattg aactcataaa tatggttatg
      781 caaaagaata atatgcacga tacagatact tttgagtttt ttaaatatat tataaagatg
      841 tttatagcag tctaccttgc aagccatgcc tttgaatttt caatggcagt ctttgatgtg
      901 gcacaaaatc ttgtaaacaa agcggcaggg gtaatcacta cttctgccac tgtttcagga
      961 gatcagatag ttgcaatggt tgatacatta aaagaaaaag aaataggtga gcttttaatg
     1021 atattagttg aaacaagcct agtgaggatt gcaattcaat gtatatcttt aactattacc
     1081 ttaatagtat atgggcgtat gtttgaaata tatgtctact catcagtatc atccatacca
     1141 tttgcgacta tgggaaataa agaatggggt cagattggaa caaattatat caagggactt
     1201 tttgccttgg gattacaagg tttgtttttg atggtatgtt taggtatcta caccgtttta
     1261 ataagaacgg tacagattac agatattcac gcaagcttgt ttagtatatt aggatatgct
     1321 ctactacttg gacttatgat gtttaagagt ggaacagttg caaaaagtat tatgaatacg
     1381 cactaggagg aataaatgtt aaaaaagaaa aattgtgctt tattaggact tgtaatttta
     1441 ttgggaggaa cttatgaaat cttaaaagag aaaaaaagaa atgaagagat tagaaactta
     1501 aagagaagaa taaataggct cggaagatgt cataatgaat ttgttatgta tcaaggagaa
     1561 tacaatgaca gaaatgaaga aagacaggaa gaattagaag aaagaattag ctacctggaa
     1621 gaagaaacaa tatctaatta tgaccatata cttgaacttt ccaaagaaga agtagaggga
     1681 gattaaaatg gcgtatgtac caataccaaa agatttggac aagattaaaa caaaggttgc
     1741 ctttaactta actaaaagac aacttatagg tttttctgtg gcaggagtaa ttggcatacc
     1801 aacctattta tttatgaaga aatatctacc taatgatgtt tctatcattg taatgctaat
     1861 agtaaccctg ccaatctttt ttataacctt atatgaaaaa gacacattaa cctttgaaaa
     1921 atattttaaa tttttctatc ttcataagtt ttatcaacca actaagagaa taagaaagga
     1981 ggcatacctt gaagcaaaga aaaaagcaaa tcagcgactt aaatctaaga gaaaaacaat
     2041 taaaaaaaga ccgaaaggaa gtaagaaagc taaaaacaaa gaaagatcca acaaatagtc
     2101 ttctaagtgt acttttaaag aaagagaaga agagattt
//
LOCUS       DAUCWU010000139         2076 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUCWU010000139 DAUCWU010000000
VERSION     DAUCWU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.140"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..633)
                     /gene="speh"
                     /locus_tag="VGY24_001719"
     CDS             complement(<1..633)
                     /gene="speh"
                     /locus_tag="VGY24_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeH"
                     /protein_id="HEQ4284672.1"
                     /translation="MRYNCRYSHIDKKIYSMIICLSFLLYSNVVQANSYNTTNRHNLE
                     SLYKHDSNLIEADSIKNSPDIVTSHMLKYSVKDKNLSVFFEKDWISQEFKDKEVDIYA
                     LSAQEACECPGKRYEAFGGITLTNSEKKEIKVPINVWDKSKQQPPMFITVNKPKVTAQ
                     EVDIKVRKLLIKKYDIYNNREQKYSKGTVTLDLNSGKDIVFDLYYFGNGDF"
     gene            complement(839..1126)
                     /locus_tag="VGY24_001720"
     CDS             complement(839..1126)
                     /locus_tag="VGY24_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284673.1"
                     /translation="MKKKLFLVSALALLTTGIALNTGSTVSASTTRLSSLNVRNILNE
                     YDRQLNWDANTYNSNLRYWMNQVWVKKATANDIRRELDKALHGEDYVSDYY"
     gene            complement(1292..2044)
                     /locus_tag="VGY24_001721"
     CDS             complement(1292..2044)
                     /locus_tag="VGY24_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ4284674.1"
                     /translation="MATLDEVLSFAKGLADAGQGVDLDNVYGTQCVDLPNWITTKYFG
                     IALWGNAIDLLDSAAAQGMEVVYNAPGVNPRAGAIFVMVTYAHGYGHTGLVIVTSDGY
                     VLHNIEQNVDGNADALYIGGPARYVDRPFEDGTGYILGWFYPPYDSTPAAVTEPSAPV
                     VAQSDGTYVANPETGTFTVRVAALNVRSAPRLDAEIVATYGENMEFNYDGWIDSDGYI
                     WVTYISVTGVRRYVSVGNSENGRRVTNFGTFR"
BASE COUNT          656 a          421 c          318 g          681 t
ORIGIN      
        1 aaagtctcca ttgccaaaat aatacaaatc aaaaacaata tctttacctg aatttaaatc
       61 taaggtaaca gttcctttag agtatttttg ttcccggtta ttatagatat cgtatttctt
      121 aatcaataac tttctaactt ttatatccac ttcctgagcg gttactttcg gtttattgac
      181 tgtaataaac ataggcggct gttgtttact tttatcccac acgtttatag gaactttaat
      241 ttcttttttt tctgaattag ttaatgtaat tccaccaaac gcttcatacc ttttccctgg
      301 acattcacaa gcctcttgtg cagatagagc ataaatatct acttctttat ctttgaattc
      361 ctgtgatatc caatctttct caaaaaaaac tgacaaattt ttatccttga cactatattt
      421 caacatatgg cttgttacaa tatctggaga attttttata ctatcggctt caattaagtt
      481 agaatcatgc ttataaagcg attctagatt atgtctattg gttgtattat aagaatttgc
      541 ttgaacaaca ttggaatata aaagaaatga caaacatata atcatgctgt agattttctt
      601 atcaatatgt gagtagcgac aattatatct cattttttct ccttaaagat tttaactaaa
      661 tgctttgttg gtgtttgttt aagtcattaa aagtaaggtc caaacattta atgaacattc
      721 gctcaaataa atacaaaaag ccctcctgta agaggttagg ggagccaaag aaaatatacc
      781 acggccgtaa caatattatc tatattaact actctattca taaattttat gttaattatt
      841 aatagtaatc actcacataa tcttctccat gaagagcttt atctagttcc cgacggatat
      901 catttgcagt tgctttttta acccaaactt gattcatcca atagcgaaga ttagagttat
      961 aagtgtttgc atcccaattt agttgacgat catattcatt tagaatattt ctaacattta
     1021 aactagatag gcgagtagtg ctagcactga ctgtgctacc agtgtttaga gcaatacctg
     1081 tagttaaaag tgctaacgca ctaacaagga ataatttctt tttcatatta attcaaatac
     1141 aactttcttt ttttatatgt cgacataata ccattatata agtttaatat aattaaatca
     1201 atattatatt atattttatt ttttgtgaaa aaacttaaaa accgcccaga aattatctga
     1261 gcggttttgt cttatctcgg agctttacct cctatctaaa agtaccaaag ttggtcacac
     1321 gtcgtccgtt ttcggaattt ccgacggaca catatcgtct aacaccagta acactgatgt
     1381 atgtcaccca aatataacca tctgagtcaa tccaaccatc atagttaaat tccatgtttt
     1441 cgccataagt tgccacaatt tctgcatcta gacgaggcgc agaacggacg tttaaagcag
     1501 caacacgaac agtaaaagta cctgtttcag ggttagctac ataagtacca tctgactgtg
     1561 caaccactgg agcgcttggc tctgtcactg ctgctggcgt actatcgtaa ggagggtaaa
     1621 accaacccaa aatatatcca gtgccatctt caaatgggcg gtcaacatat cgagctggac
     1681 caccgatgta aagggcgtca gcattaccat ccacgttttg ctcgatgtta tgcagaacat
     1741 atccgtctga cgtcacaata actaatccag tatgaccgta accatgagca taggtcacca
     1801 tcacaaagat agccccagct cgtggattaa ctccaggagc attatagacc acttccattc
     1861 cttgggcagc tgcactatct agtaagtcaa tagcattgcc ccaaagggca atgccaaaat
     1921 attttgtcgt gatccagttt ggcaagtcca cgcactgcgt accgtaaaca ttatcaagat
     1981 caactccttg gccagcatcc gctaatcctt ttgcaaaaga caagacttcg tctaaagttg
     2041 ccatatcagt tacttccttt cttcaaaaaa tgggct
//
LOCUS       DAUCWU010000140         2061 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUCWU010000140 DAUCWU010000000
VERSION     DAUCWU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.141"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..204
                     /locus_tag="VGY24_001722"
     CDS             <1..204
                     /locus_tag="VGY24_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284675.1"
                     /translation="PNYFHVQFKLGEKVSYNTPSAEGREVIPIKGAEVTKMIFADGNE
                     LLSVIHNETVDVYASFPIVLEYH"
     gene            complement(314..514)
                     /locus_tag="VGY24_001723"
     CDS             complement(314..514)
                     /locus_tag="VGY24_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284676.1"
                     /translation="MTRFAIQRLREEKKMSQEELANKSGVSRTTISLIETDKSTTVKL
                     STLQKLAVALDVPIGYFFKHNV"
     gene            607..1248
                     /locus_tag="VGY24_001724"
     CDS             607..1248
                     /locus_tag="VGY24_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284677.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            complement(1347..1505)
                     /locus_tag="VGY24_001725"
     CDS             complement(1347..1505)
                     /locus_tag="VGY24_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284678.1"
                     /translation="MNWKKLMLGNLEHTFTSHDGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            1862..>2061
                     /locus_tag="VGY24_001726"
     CDS             1862..>2061
                     /locus_tag="VGY24_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284679.1"
                     /translation="MKLGEIIKNFREEKKLSMDRFAEKSGLTKGYISMLEKNEHPKSK
                     KPIIPTEETLLKVAKGMGVDID"
BASE COUNT          653 a          362 c          349 g          697 t
ORIGIN      
        1 cctaattatt ttcatgtcca atttaagtta ggcgaaaaag tatcgtacaa cacgccttca
       61 gcagaaggaa gagaagtcat ccctatcaaa ggtgctgaag ttacgaaaat gattttcgcc
      121 gatggtaacg aattgttaag cgttattcac aacgaaaccg ttgacgttta cgctagcttc
      181 ccaattgttc ttgagtatca ttaattcgtt atttctaaac cagcaatcgc ccgctactgc
      241 gtttgttggt ttttcttttc cgtaaaaaat tcgatttgct tgcatagtgt cctttccagt
      301 tttgtttaac acgttaaaca ttatgtttaa aaaaatatcc aataggtacg tctaaagcga
      361 ctgccaattt ttgaagagtg cttagcttaa ccgtagtaga tttatcggtt tcaataagag
      421 atatagtagt tcgtgaaact ccagatttat tggctagctc ttcttgagac attttctttt
      481 cttcacgcaa tctttgaatt gcaaacctcg tcattccccc acctcctttc taatttggaa
      541 ttatccaaaa caacatagct ttaaaatttt ctgtggtata atttaaataa aaattgtgag
      601 gttgaaatga atttttttaa ttttttattg tgtgttttta agtttacaag tgaatatcta
      661 ataaaaaatt ggatagcttt aatagctctg tttctatctt attcaaacta ccgaagaaat
      721 aacttacaag tcgagttaat tgctgctcct gtttcagatt ggattttgag cgttatttta
      781 gacaacggtg aaagcatata taatccaaat ggtacattaa gagctaacat taaaatcatc
      841 aatccttcta atgttgatgt aagctacttc gacttgattg tttttgataa aaacagaaaa
      901 tatcagcatt attaccaaaa gcaaaataat ataattaacg atttaacagg tagagaggct
      961 atagccgcag tacagcctga tggcaataca atccttatcg aggttccaga ggcagattgt
     1021 ggagtattaa aagcccacag tatgacaagg atggatttaa tcatacaaac gtctgaaatc
     1081 acagatagac tctttgttgc ttttaaagta gctaaaaaga aaaaactatt taaagctaat
     1141 aaagcaggat atgttaattc accttatcaa tcattttctg cgtcattccc tgtggaatta
     1201 tcaaaaaaac cgcactacga ggatatccta aaagatttgc atgagtgaga gcagattttc
     1261 ttgtgtgaaa tatcttggaa gaacctagta caccgtattt aattttttcc atgccttccc
     1321 tcctttccac tccttttggg gagtttttat tttgtaataa accaagcaat cagccaagtg
     1381 ataccgccta gcactaacag cgctggtaat acgccgcctt caaattcgat gcttgttttt
     1441 tctttgccat catgactagt aaacgtgtgt tctaagttgc caagcattag ctttttccaa
     1501 ttcatgcaac ctctcctttc attcttgcgg agatacagcc aatgtgctaa actaaactta
     1561 ccccgttagg gggagaggac ttcttagccc tctaattatc ctcaccactc tattgagtag
     1621 tgaatcttaa gcttaaacca aagaatcttg atttcgactt ctagttcttt gcgtttaggc
     1681 tttttgttta gcctagattt catcagctgt acctcctttc gttttgctta atcccttaag
     1741 cttgattata gtttaacacg ttaaacattc attgtcaagc gtgttaaaca aaaatattga
     1801 attatttttt gttgtgttgt ataatgtatt aaacaatcat tctaggaaga ggtttttaac
     1861 attgaaatta ggggaaataa taaaaaattt cagggaagaa aaaaagttat caatggatag
     1921 gtttgctgaa aaatctggtc ttactaaggg gtatatttca atgcttgaaa aaaacgagca
     1981 tccgaaatct aaaaaaccaa ttatccctac agaagaaact ttgttaaaag tagcaaaagg
     2041 gatgggggtt gatattgatt t
//
LOCUS       DAUCWU010000141         2002 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUCWU010000141 DAUCWU010000000
VERSION     DAUCWU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.142"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            87..1049
                     /locus_tag="VGY24_001727"
     CDS             87..1049
                     /locus_tag="VGY24_001727"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ4284680.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            1235..1990
                     /gene="recO"
                     /locus_tag="VGY24_001728"
     CDS             1235..1990
                     /gene="recO"
                     /locus_tag="VGY24_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ4284681.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFTHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
BASE COUNT          626 a          300 c          398 g          678 t
ORIGIN      
        1 acattcttta acggtttttc aatttcattt gataattcac ttgaaaagcg ttaaaatggt
       61 ataggattaa aactttggag gaaaaaatgt cttattctga tttaaaattg tttgctttat
      121 cgtcaaacaa ggaattagca gaaaaagttg cgtctgcaat gggtattcaa cttggaaagt
      181 ctacggttcg ccagttttca gatggtgaaa ttcaagtaaa tattgaagaa tcaattcgtg
      241 gtcatcatgt ctttattttg caatcaacta gctcacctgt caatgacaat ttaatggaaa
      301 ttttgattat ggtcgatgct ttaaagcgtg ctagtgctga aaaaatttca gttgtgatgc
      361 cttactacgg ctatgcacgc caagaccgta aagcgcgttc tcgtgaacct attacttcaa
      421 aattagttgc taatatgtta gaagttgctg gtgtagatcg cttattaacg gttgatttac
      481 atgctgctca aattcaaggg ttctttgata ttccagttga tcacttgatg ggtgcacctt
      541 taattgcaga ctattttgat cgtcatggtt tagtgggtga agatgttgtc gttgttagtc
      601 ctgaccatgg tggtgtgact cgtgcgcgta aattagcaca attcctccaa acaccaattg
      661 caattatcga taagcgtcgt agtgtggata agatgaacac cagtgaagtg atgaatatta
      721 tcggaaatgt ttcgggcaag aaatgtatct tgattgacga tatgattgat accgcaggga
      781 ctatttgcca tgcagcagat gcgcttgctg aagcgggggc aacagctgtg tatgcgtcat
      841 gcacgcatcc cgttctttct ggaccagcac ttgataatat tcaacgttcg gctatcgaaa
      901 aactaattgt tcttgatact atttatctgc caaaagaacg tcttattgat aaaattgaac
      961 aaatttcaat tgctgatttg gttgcagaag cgattattcg tattcatgaa aaacgtccgt
     1021 tgtcaccttt atttgagatg ggcaattaaa cttaacgaag cctgagataa tagatctcgg
     1081 gctttttgac ttgaataaaa tgtaagggta atgtggtatt aattattgtg ggatgaacta
     1141 tgcaattcta aaggattttg ggtataataa gaaagataac aacagtttat tgtaaactaa
     1201 gataaaaggt aagagaaaaa tacgacgagg taatatgcaa ctaacagaat cactaggcat
     1261 cgttcttttt aataggaatt atcgagaaga tgataaatta gtcaaaatat ttactgaagt
     1321 agcaggtaag cagatgtttt tcgtgaaaca tattagtcgt tccaaaatgt cctcaatcat
     1381 tcaaccacta acgattgctg attttatttt caagttaaat gatacaggct tgtcttatgt
     1441 tgttgactat agtaacgtta acacttatcg gtatattaat aatgatattt ttcgattagc
     1501 ctatgctagt tatgtcttag cattagctga tgctgcgatt gcagataatg aatcagattc
     1561 gcatttgttt acgtttttga aaaaaacact tgatttgatg gaagagggcc tagattatga
     1621 aattttgaca aatatttttg aaattcagat attagatcgt tttggtatta gtctaaactt
     1681 tcatgagtgt gccatttgtc atcgcactga tttaccgctt gattttaccc atcgtttttc
     1741 agctgtactt tgttctgaac attattacaa agacaaccga cgtaatcatt tagatccaaa
     1801 tgttatctac ttgttgagtc gatttcaaaa aatcacattt gatgatttga gaactatttc
     1861 attgaataaa gacatcaaaa agaagcttcg tcagttcatt gatgagttgt atcacgacta
     1921 tgtaggaatc aaattaaaaa gtaaaacatt cattgataat ttagttaagt ggggagatat
     1981 tatgaaatga tagggtctct tt
//
LOCUS       DAUCWU010000142         1966 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUCWU010000142 DAUCWU010000000
VERSION     DAUCWU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.143"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..705
                     /locus_tag="VGY24_001729"
     CDS             <1..705
                     /locus_tag="VGY24_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ4284682.1"
                     /translation="LKSLIEREILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTT
                     LNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDL
                     LNINNIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQ
                     IHFEIWNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNN
                     PARGGASAIISIKKKI"
     gene            862..1002
                     /locus_tag="VGY24_001730"
     CDS             862..1002
                     /locus_tag="VGY24_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ4284683.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
     gene            1117..>1966
                     /locus_tag="VGY24_001731"
     CDS             1117..>1966
                     /locus_tag="VGY24_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284684.1"
                     /translation="MIKAQLKNLIKTIHLVKEAGFSLLVFSIAISLFQGILPIFSMLL
                     VQKMLNIITTDIKNFHTLMIAFISYIALTLLTIIIGEVDSYIDTKLQILLHYKMNHLV
                     MQKTVKLTLAEFEILYKRSSDERANWYISHLLTHDFAVKELRLGTLESYFLKKYDKNN
                     QLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFLGILLVGNVTTFIRSLS
                     TMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQSVEFERVSFS
                     YNGKDNV"
BASE COUNT          709 a          236 c          339 g          682 t
ORIGIN      
        1 ttgaaaagtt tgattgagcg agaaatattg gaaaagcaag atctatcctt tcaaattgca
       61 gcactttcgc atgatataaa aacaccttta acggttttga aaggaaatct tgagttatta
      121 gaattgacta ctttaaataa aaatcaggaa ggttatattg tatctatgaa caatagtatt
      181 tctgtttttg aaggctattt caattcttta atatcttata cacgcatgct ttcagaggat
      241 agatcagtga aactaatttt agttgaggag ctattaagtg agttacattt tgaagtcgat
      301 gacttattga atatcaataa tattgaattt tctatttgta atagacttat tatcacttct
      361 ttttatggag atgaggaaaa tctgataaga gctctttcta accttttagt taatgcaata
      421 agatttatgc ccgtattaga taaaaaaatc gaagttatac tgtctgagtc aggagagcaa
      481 attcattttg aaatatggaa taatggtgag cgtttcagtg atagtacact gaaaaaggga
      541 gataaattat tttacacgga agattatagc agaggtaaca aacattatgg aatcggtttg
      601 gcatttgtta aaggcgttgc tatcaaacac ggtgggaatc ttcaattgaa taatccagct
      661 agaggaggtg ctagcgccat aatctcgata aaaaagaaaa tttaatagat ttgattgaaa
      721 ctggagtatt tcaggcaaaa aatcgtaaat tttggtgatt tttggctaaa atactttttt
      781 tctgaacaaa ttctgaactt ggggtgtcat aataatcatg taaatatatc ggatgcaaaa
      841 acgtaagaga ggaactagaa aatgaataac acaattaaag actttgatct cgatttgaaa
      901 acaaataaaa aagacactgc tacaccttat gttggtagcc gttacctatg tacccctggt
      961 tcttgttgga aattagtttg ctttacaaca actgttaaat aataaagtta tcattatcat
     1021 caacattata atttcaaata atttgatata tttataaatt taggggagta gactgatttt
     1081 agtttgctcc tttattagta gataaggaag ttatacatga ttaaagctca gctaaagaat
     1141 ctaataaaaa caattcatct cgtaaaagag gctggtttct ccctactggt gttctcaatt
     1201 gctattagct tatttcaagg gattttaccc atttttagta tgctactagt tcaaaaaatg
     1261 ttaaacatta taacgactga tataaaaaat tttcacacat tgatgattgc ttttatctca
     1321 tatattgctt taacattatt gacaattata ataggagaag ttgatagtta tattgatact
     1381 aaattacaga tacttcttca ctataagatg aaccatttag ttatgcagaa aactgtaaag
     1441 ttaacattgg ctgaatttga gatactatat aaaaggagta gcgatgagag agcaaattgg
     1501 tatattagtc atcttttaac acatgatttt gctgtcaagg agttgaggtt aggtacgtta
     1561 gaaagttatt ttctaaaaaa atatgataaa aataatcaat tatttattaa tcaggaaaat
     1621 agtataaata gatctaaaat gaaatacggt atagtattag gtgtattgga gattgctata
     1681 caatctatta ttatgttcct tgcagttaga gaagcatttc taggaatatt attagttgga
     1741 aatgtaacta cttttataag gtctttatct acaatgcaaa caagtacaca aaatatagta
     1801 aataatatct attctttcta taatggttca ttgtacatgg agttattgta tgattttacg
     1861 catgatgagt tagtacataa ccctataatc gaaagtacgg ttgatacaat tcagtcagtt
     1921 gaatttgaaa gagtttcttt ttcatataat ggtaaggata atgtaa
//
LOCUS       DAUCWU010000143         1885 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUCWU010000143 DAUCWU010000000
VERSION     DAUCWU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.144"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1470
                     /locus_tag="VGY24_001732"
     CDS             <1..1470
                     /locus_tag="VGY24_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEQ4284685.1"
                     /translation="VLLSGAKSSELVRDYLSSGSDNTGVESGEKVANVSSKLQHGIRK
                     YKLDKKKKSLKKLSKLDKKIRNRKSKLEFKSGLEDLKKSDAYIKKNRFKKFYQRKQMK
                     SMIAKKHETRLVDRVKKAILSLGKASKELIVRKSKMVLFLVIGLGLMLSIMIGGGSVG
                     MSGLSNSVNSVMTTTYLSQDTVLSEVNQEFSSMEYDLQSQIESVKTSHPGYDEYIINK
                     EGEIGHNTHELLSYITSRCGEVKSASEVKGILKEFFDKMYKLDFKEEIEIRTRTVART
                     RYDSRGNPYTSYEKEEYEYKKLIVTLKKKEMDEVVREIFKDYPDNVVHYEALLEAKGN
                     MGDVFGSGNGDLGEIVDNPNFGNPGLAFDSATAKALFNEAEKHIGKRYVFGASGPSNF
                     DCSGFVCWSFTKSGVKSMPRTTAWRIYKDYCNPVSPSEAQPGDIIFFHSTYNSGTPIS
                     HVGIYAGNGMMIHAGDPIQYTSINSKYWKSHFYGFGRPR"
     gene            1485..1724
                     /locus_tag="VGY24_001733"
     CDS             1485..1724
                     /locus_tag="VGY24_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284686.1"
                     /translation="MNRKLISIRNKKKKMEEKLKDLNAKYKEICDEEIQVENEEIIVT
                     LRRNNISLEELMEKINDRKREEKLKEKENIHNEEI"
     gene            1711..>1885
                     /locus_tag="VGY24_001734"
     CDS             1711..>1885
                     /locus_tag="VGY24_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase"
                     /protein_id="HEQ4284687.1"
                     /translation="MKKFNTNRLRAFFMALLLVLTSTSTTNVLAKADDTDGSKKVIES
                     SISKISDLENQIKD"
BASE COUNT          785 a          222 c          372 g          506 t
ORIGIN      
        1 gttttattat ctggagctaa gtcttcagag ttagtgagag attatttaag ttcaggatct
       61 gataatactg gtgtagaatc tggagaaaag gtcgctaatg taagctctaa actccagcat
      121 ggaataagga agtataagtt agacaaaaag aaaaagtcct taaaaaagct atctaaactt
      181 gataagaaaa taagaaatag aaaaagcaag ttggagttta aaagtggctt ggaagattta
      241 aaaaaatcag atgcctatat aaagaaaaat agatttaaga agttttacca aagaaaacaa
      301 atgaagagca tgatagctaa aaagcatgaa acaaggcttg tagatagagt taaaaaagct
      361 attttaagtt taggtaaggc ttctaaagag ttaatcgtaa gaaaaagcaa gatggtttta
      421 tttctagtaa taggattagg tcttatgcta tcaattatga ttggcggcgg aagtgttggt
      481 atgagcggac taagcaattc agtaaactca gttatgacaa caacatacct atcacaagat
      541 acagttctaa gtgaagtaaa tcaagaattt tcatcaatgg aatatgacct acaatcacaa
      601 atcgaaagtg taaaaacaag tcatcctgga tatgacgaat atatcataaa caaagaagga
      661 gaaattggtc ataataccca cgaactttta tcctatataa cttcaagatg tggagaagta
      721 aaatcagcat ctgaagtaaa aggaatttta aaagagtttt tcgacaagat gtataagctt
      781 gattttaagg aagaaattga aattagaaca agaacagtag caagaacaag atatgatagt
      841 cgtggaaatc cttatactag ttatgaaaaa gaagagtacg aatataaaaa gttaattgta
      901 actttaaaga aaaaagaaat ggatgaagtt gttagggaaa tatttaaaga ttatccagac
      961 aatgtagttc attatgaagc tcttcttgaa gctaagggaa atatgggaga tgtttttgga
     1021 tcaggaaatg gggacttagg agaaatagta gacaatccaa actttggaaa tcctggtctt
     1081 gcttttgatt cagctactgc aaaggctctt tttaatgaag cagaaaaaca tataggcaaa
     1141 agatatgtgt ttggagcaag tggaccatct aactttgact gctcaggatt tgtatgttgg
     1201 tcatttacaa agtctggtgt aaagagtatg ccaagaacaa ctgcatggag aatatacaaa
     1261 gactattgca atccagtaag tccaagtgaa gctcaacctg gagatattat atttttccac
     1321 tcaacatata acagtggaac accaatatct cacgtaggaa tatacgcagg taacggaatg
     1381 atgattcacg caggagatcc aattcaatac acatcaataa attcaaaata ttggaaatca
     1441 cacttttatg gttttggaag accaagatag aagggagata ttatgtgaac agaaaactta
     1501 taagtattag gaataaaaag aaaaaaatgg aagagaaatt gaaagattta aatgcaaaat
     1561 ataaagaaat ttgcgatgaa gaaatacaag ttgaaaatga agaaataatt gtaactctta
     1621 ggaggaacaa tattagcttg gaagaattaa tggagaaaat caatgataga aaaagagaag
     1681 aaaaattaaa agaaaaggag aacattcata atgaagaaat ttaatacgaa cagactaaga
     1741 gcctttttta tggctctttt attagtttta acatcaacct caactactaa tgtactagct
     1801 aaagctgatg atacagatgg cagtaaaaaa gtgatagaaa gttctattag taaaattagt
     1861 gatttagaaa atcagatcaa agact
//
LOCUS       DAUCWU010000144         1879 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUCWU010000144 DAUCWU010000000
VERSION     DAUCWU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.145"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1209)
                     /locus_tag="VGY24_001735"
     CDS             complement(<1..1209)
                     /locus_tag="VGY24_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEQ4284688.1"
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFD
                     KSLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGI
                     ALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSG
                     ITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPML
                     ILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMA
                     WCYGTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIY
                     DLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVS
                     GIVLFLLNPYS"
     gene            complement(1193..>1879)
                     /locus_tag="VGY24_001736"
     CDS             complement(1193..>1879)
                     /locus_tag="VGY24_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284689.1"
                     /translation="ERLAIVGENGSGKSTIFKLACGLYDNYEGNIYINGINLRSIQKK
                     SYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTGVDSIFYFNQEKL
                     NQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSSALDPESETKIF
                     KTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCISETGSFADLNIKGMKFKNLLDK
                     EIGATYDTSN"
BASE COUNT          619 a          356 c          222 g          682 t
ORIGIN      
        1 actgtaagga tttaataaga ataacactat ccctgatacc ccttgcaata tccctattga
       61 ctccagttca acttctattt ctccaaattt ttctctgtca gtatacccaa atacttttgt
      121 agttgaaaac gatgtaatta tttgttggcg aagctcttct gcaaagcgag ttagcctctc
      181 acttgaataa tttttaccaa tcaagtcata tataaaagtt actcctgcca aaccatggca
      241 cattgtaaga gaatagacat ttttgtctga acttctcatc tgagcaatta aactttcatt
      301 aatatcgtca ataaaattta aatctatcaa ttgacacatt ttaaaaaggg caactgcagt
      361 tccaggcgta ccgtaacacc atgccattct agtaggaaga taatttaaat caagatccaa
      421 aatattacta gtataaattt ttccaggcca tagtttgtca tcccttctaa ataaaaacat
      481 taaatttata gctttattca gtatatctcc tacttctaca ctcaaaattt tttctgattt
      541 taattttgct aggattagca taggaccagt aattccatgt gcaattccta gatcaaattc
      601 agcttctcta tcttcagaac aattagttac cttatttaaa attgtctcaa tatatttcaa
      661 aagataattt ccaatttgat ttaactctac agaaaaaaat tcttgttcaa ggagtaagta
      721 attagttatt ccactaaatc cgttaaccat atcataatca tatggctcaa taattccttt
      781 ctcaatatta gaaaaactta ctaccaattt gtctttaatt tgttctttta gaagacaatt
      841 taaggatgat aataatttcg gataatactt tccagatata ctcgcttctt taatagctaa
      901 tgcaatacct gcagtgccag catagagaga cggagattct ataccatctc tagagattat
      961 gtctactaat ttctcaacgt atatgttgga gaatgttaga tatctctcat cttttgtcac
     1021 tttatatagt gaactataca ttaagattaa tgatggcaca ccatgactca gagatttatc
     1081 aaaaaaggta tttctatatt tagattcaat attaattagt actttattct cactttcagc
     1141 tactatctgt tcataattta taaaactatc tgcaatgtaa tctactatct gtttaatttg
     1201 aggtgtcata tgtggctcca atttctttat ctaataagtt tttaaacttc attcctttaa
     1261 tatttaaatc agcgaaactt cctgtttcag atatacatcc attatctaga tacaaaattt
     1321 tattcacaga ttgagtaatg cttggcttat gcgtaataaa aataccaatt ttattttgcg
     1381 ataattcaaa aaatgtttta aaaatttttg tttcagactc aggatctaac gcagaactag
     1441 gctcatctag caaatagcaa tccgcttctt tgagatatac tcttgataat gcaatttttt
     1501 gccactgtcc accagataac tgtctcccat cctcaaacca attccctaat tgctgttcca
     1561 gatctatctg atttaatttt tcctgattaa agtaaaatat cgaatcaact cctgtatact
     1621 tcagtgcact tattaaatcc tcatcactat ataactttga taattcaccc aatccaacat
     1681 tttcacgcag tgtcaattca tactttaaaa aatcttggaa caatgcggat attctcttgt
     1741 agtatgactt tttctgtatt gaacgaagat taataccatt tatatatata ttgccttcat
     1801 aattatcgta aagaccgcag gctaatttga aaatcgtact ttttccagaa ccattttcac
     1861 caacaattgc taacctctc
//
LOCUS       DAUCWU010000145         1863 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUCWU010000145 DAUCWU010000000
VERSION     DAUCWU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.146"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            12..>1863
                     /gene="cadA"
                     /locus_tag="VGY24_001737"
     CDS             12..>1863
                     /gene="cadA"
                     /locus_tag="VGY24_001737"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ4284690.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKAK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLL"
BASE COUNT          529 a          339 c          420 g          575 t
ORIGIN      
        1 gggaactaaa aatgacaatc agacaatgga tggcagacca tcttcatttg atggagactt
       61 tagcgtgttt ggtattaata ataattggcc tagccttttt acactcattt ccacaagtgg
      121 cttccgctat tttcattaca gccttcttga ttggaggata tgcgtctgca aaaacgggta
      181 tattggattt ggtgaagaac aaacacttgt cagtggatat tttgatgatt ttggcagcta
      241 tcggtgctgg aattatcggc tattggctgg agggtgctct gcttattttt atcttttcgt
      301 tgtccaatac gcttgaagaa atggccatgg aaaaaagtaa ggatgctatt tcagccttga
      361 tgtccttgac gccagataca gctcgtcaat accaagaaga tggccatatt ttagaagttg
      421 agaccagatc cttgagtgtt ggtgaccgtt tacaagttcg taaaggcgaa gctgttccaa
      481 ttgacgggca gttgcttagt ccttttggtc aatttgatga atctatggtc actggtgagc
      541 ctatcactgt tgataaggca gaaggccagg atctgattgg gggaaccatt aaccaaggac
      601 aaaccataga tatgttggtt actattgaaa atgacgatac tctctttgcc aagattatta
      661 atctggtgga atctgcccaa gaaaagaaaa gcaaaaccgc cacctttatc gaaagcttag
      721 aagatggtta tgtcaaattt gtgctcgtcc ttattcctgc ctttatcctc tttagtcact
      781 ttgtgctttc ttggacttgg ttggctgctt tttaccgagg gatgattctc ttaacagtag
      841 cttcaccatg tgccctaatt gccagttcca cacctgctag cttggctgct atttctcgtg
      901 cagccagaaa gggattgatt atcaaaggag gggatattgt cgataacatg ggagatatta
      961 aggctgttgt catggataaa acgggaacgc tcacccaagg aaaaccttct gttgtgaatg
     1021 ctcattattt ggaagatgag ttgctcgtga atagactggt aaaaggagca gaggctgcta
     1081 gtacccatcc tatttctaaa gcccttcttg aatacactga aaagttggag ccactgacct
     1141 ttgaccactt agaagaaatt tctgggaaag gttttcaggg cttctatcaa gggcaagaat
     1201 ggcgaattgg caagaaaacc ttcattttgg aaaaggttca agacctatca gcttttgaag
     1261 aaactattca agtggaagaa aatcaaggga aaaccctaat ctttgtttca cgtgaccatc
     1321 aattgatagc ttactatgcc ctcttggatg atatcaaaat agaatcaaaa cgtgctatta
     1381 agtctcttca tgccatggga atcaaaacag tcatgttaac aggtgaccaa gaacgaaccg
     1441 ccaattatgt ggcacaaaaa cttggtattg atgaagtggt agccaactgt atgcctcaag
     1501 ataaggtggc taagttagca gaattaaagg ctaaatatgg ttttgtggct atggtaggag
     1561 atggtattaa tgatgctcct gcccttgctc aagcagatgt ttcttatgct attggatcag
     1621 gaacagatat tgcaatggaa agtgcagaca gtgtgattat ggatgacttg actcgtattc
     1681 cattttcgat tcaactttcc cgcaaaatga agaccattat caaacaaaat attgtttttg
     1741 ccttatctgt gattacctta ttgattttag ccaatgtttt tcaggtagtt aacttgccgc
     1801 ttggtgttgt tggacacgaa ggctcaacga ttttagtgat tttaaatggc ttgcgtttac
     1861 ttt
//
LOCUS       DAUCWU010000146         1819 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUCWU010000146 DAUCWU010000000
VERSION     DAUCWU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.148"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            173..1777
                     /locus_tag="VGY24_001738"
     CDS             173..1777
                     /locus_tag="VGY24_001738"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ4284691.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
BASE COUNT          554 a          311 c          423 g          531 t
ORIGIN      
        1 agaggtcagg caaaagctct ctagttaata gggggctatt tttgttttct agaataatgc
       61 cagtttgcct ctatatttag aaaactaagg tgatacctaa ccaatgtgga accaactaat
      121 agggaattaa caagaaagca atgcttttat tcagcttata taaaggagta ctatgacgaa
      181 atacattttt gtaactggtg gtgttgtatc atcaattggc aaaggaattg ttgcggcaag
      241 tcttggccga ttgttaaaaa atcgtggact taaggtgacg attcaaaaat ttgatcctta
      301 tattaatatt gatccaggga cgatgagccc ctatcagcat ggagaagtat atgtgacaga
      361 tgacggtgct gaaaccgact tggatttggg tcactatgaa cgttttattg atatcaatct
      421 taataaatat tccaatgtta caacaggtaa aatttatagt gaagtccttc gtaaagaacg
      481 taaaggcgag taccttggag cgactgttca ggtgattcct catatcacag atgcccttaa
      541 agaaaaaatc aaacgcgcag cttcaacaac ggactctgat gtaattatta ctgaggtcgg
      601 ggggactgtt ggcgatattg aaagtttacc gttcttagaa gctcttcgtc agatgaaagc
      661 tgatgttggt tctgaaaatg tgatgtatat tcacaccacc ttgttgccat atttaaaggc
      721 agcgggtgaa atgaaaacta agccaacgca acattccgtt aaagaattgc gtggtttagg
      781 gattcaaccg aatatgttgg ttattcgtac ggaagagcca gttgagcagg ggattaaaaa
      841 taaattggct cagttctgtg atgtgaattc agaagctgtt atcgagtcac gtgatgtaga
      901 acacttgtat caaattccat tgaacttgca agctcaatca atggatcaga ttgtttgtga
      961 tcatttgaaa ttgaatgcgc cacaagctga tatgaccgag tggtcagcta tggttgacaa
     1021 ggtgatgaat ttaagaaaaa caacaaaaat tgccttggtt ggtaaatatg tggaattgcc
     1081 agatgcctac ttatctgttg tggaagcctt gaaacattct ggttatgcca atgataccgc
     1141 aattgaccta aaatgggtca atgctaacga tgtgacggtg gataatgcag ctgacttgtt
     1201 gggagatgcg gatgggatta ttgtaccagg tggttttggt caacgtggta cagaaggcaa
     1261 gatccaagcg attcgctatg cccgcgaaaa tgatgtgcca atgcttggta tctgtcttgg
     1321 catgcagttg acctgtgttg aatttgcccg ccatgtcctc aatatggaag gtgcaaactc
     1381 ttttgaatta gagccaagta ctaaataccc catcattgat atcatgcgtg atcaaattga
     1441 tattgaagat atgggaggta cgcttcgtct gggattgtac ccatgtaaat tgaaaccagg
     1501 ttcaaaagca gcaatggctt acaataatca agaggtggtg caacgtcgtc atcgtcaccg
     1561 ttatgagttc aacaacaaat tccgtccaga atttgaagca gctggttttg ttttctcagg
     1621 ggtatcacct gacaaccgct tagttgaaat tgtagaattg aaagagaaaa aattctttgt
     1681 agcagctcaa taccacccag aattgcaaag ccgtccaaat cgtcctgagg agctatacac
     1741 cgcttttgtt acagctgcta ttaaaaatag caattagtgg ttttaagggt aagtttgtgg
     1801 cattctactt ggctggtag
//
LOCUS       DAUCWU010000147         1819 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUCWU010000147 DAUCWU010000000
VERSION     DAUCWU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.149"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            93..790
                     /locus_tag="VGY24_001739"
                     /pseudo
     CDS             93..790
                     /locus_tag="VGY24_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
     gene            941..1627
                     /locus_tag="VGY24_001740"
     CDS             941..1627
                     /locus_tag="VGY24_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ4284692.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
BASE COUNT          663 a          230 c          361 g          565 t
ORIGIN      
        1 ataaagagga ggtatccact gaagagagta aggaagtctt caagaaatac aaggggcaac
       61 gagatgccat ccatgagttt gaagttgaac ttgtggaaca tcaaattgag aggttaattg
      121 ctgaggacat ttatatcaag gtgtcttttg gtgtaaagca gagtggtctt attttcattc
      181 ctaactatca attggatatt aggaaagaag aaaatcataa aaaatataaa gtctatattc
      241 gtgagaccgc acaattcttc atttacaaca aggaggcgtc taaacttaat cgctatgtgc
      301 gagggcgtga gctcatttac cagttaacca atgatagcaa gagtattcca aaaagaagac
      361 gaccaaaacc attgatacac ttaagaaaaa gattgaagaa ataagtctcc tcattgagtt
      421 agatacggag aataagcctt atcaagatat taaagatgac attgttaaag atatggctca
      481 attagatttg accattacag agttgcagga ccatattgct catctcaata aggttgcgga
      541 ggtcttgctt aatctgaaca acaacgatat agagaaccgc cgtttggcca gatatgacta
      601 tgccaagatg aacttgactg cagctataaa aatagaagaa gttgagaaag agattgaaac
      661 ttctcaaaat gaacttaata tatccataga tgagtatgaa tatctagtaa gaaggttgga
      721 aaagtttgga gagatcttga gtgatagcaa aattatcgat acttctcgaa atgaaataca
      781 atgggagtaa ttatgatata atttgggtgg tagcaatttt tatatcagat attaaaatct
      841 agttgatgtt tcgtaaataa ctatattaaa gaaatgattc ctgccgctta cattaagaat
      901 agtttatttg taagtatttc attatttaaa aggggtaata atgtataaga ttttagccat
      961 agatgatgat aaagaaattc tgaagcttat gaagactgct ttggagattg aaaattatca
     1021 tgttataacc tgtcaagaga tagaattacc aatagttttt gatgatttta aaggatatga
     1081 tttgatctta ttggatatca tgatgcctaa tataagtgga actgagtttt gttataaaat
     1141 tcgggaagaa gttcattctc caattatttt tgttagcgct ttagatggcg ataatgaaat
     1201 tgtccaagct ttaaatatag ggggagacga ttttattgtg aaaccattta gcttaaaaca
     1261 attcgtagct aaagttaact ctcatttgaa gagagaagag agagcaaaga taaagaatga
     1321 ggctgaggag agagtgaagc gcagttttcc acctatagaa atctatctag aggaacgtat
     1381 gttatatatt gataaacagc cgttattcct gacttataga gagtacgaaa ttttagaatt
     1441 actgtcacgt catccgtata aagttttcac aaaagaagag atatatgaac aagtatatag
     1501 cgatgaagct tcagcattgt ttcattctat ttcagaatat atctatcaga ttagaatgaa
     1561 attttcaagt tttggaatta atccaataaa aactattcgc gggattggtt ataaatggga
     1621 tgtttaaaaa atattcaata aaaaatcgtg tttggcgcgc cgtcgttgaa attgtctttg
     1681 gagtttgtat cactgtttta atgattgcga taatatcgct ttctttttca aagttaaata
     1741 tagtgacatc tcataatgtg ggagaagaat tttatattaa agataaacag tcaatcaaac
     1801 agttgaacaa ttatatgaa
//
LOCUS       DAUCWU010000148         1754 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUCWU010000148 DAUCWU010000000
VERSION     DAUCWU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.150"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..568)
                     /locus_tag="VGY24_001741"
                     /pseudo
     CDS             complement(1..568)
                     /locus_tag="VGY24_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(566..876)
                     /locus_tag="VGY24_001742"
                     /pseudo
     CDS             complement(566..876)
                     /locus_tag="VGY24_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(1036..1656)
                     /locus_tag="VGY24_001743"
                     /pseudo
     CDS             complement(1036..1656)
                     /locus_tag="VGY24_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
BASE COUNT          513 a          384 c          276 g          581 t
ORIGIN      
        1 tgagtatctt cttctaggat aaaagttaca ttttttgttt taagtcaatg gttagattca
       61 cttcttttgc ttttaatagt agctcctcta atgaattggc acggttaaac aaaaatttta
      121 atcgactttc aatttctatc ttggcaaaat gtgtacgaaa aaattcttca tcatataaat
      181 ctcgtttact gagttgacga cctcgaattg gttttttcat tactcgatcc gtcatcatga
      241 atctgctatg cttctgacta aaatcaatat gaacatgtaa ttactctgct ttttctaaaa
      301 aatcatcaaa gaactttgac tgctgcatca aaaaataaag acgttgcttt agttcaaact
      361 tatggctagt cgctctatat ttttgatagt cacggtaaga gaaatgcttt tcaataattt
      421 tcgccccagc cattttagaa atgcggtctg aaatcatacg aaggtttcgt tccaaggcat
      481 aattccatat caacttttta tctgaattac agtcaatggc gttgattaag atgtgattat
      541 gaatatgatc tttgtctgta tgagtcgctc gagtaatctc aaagaagaga gttctcctag
      601 tgccctcaaa tcccagtcgt ctgcctgcga gtgaaaatgc ttgacaaggg aaaaaccacc
      661 gcagatgata tccacttgcc ctctaagttg tctaaagtct tggtctgtga cttgtctaat
      721 gtcatgaaat tctatttctc cttcggtctc atagatggct ttgtaagatt gcctcgcaaa
      781 cttatcaatc tcacaaaagc caatacactc atggcactga ttaattaaac ccaatcgaaa
      841 gcctccaatt cctgcaaaca aatctaaaaa cttcatatct ctcattcctt tctgacaaaa
      901 taaaaggacc ttactgctta tgtaacgtcc ttactcaact ataatcttcc atttctggtg
      961 cagtgataac ttcaataatc aaccaaattg tccttagacc ccaaacacct aataatagcc
     1021 accaaaacat cttactcacc ttagttgctt ggtccattcg gataagacac cattaaagac
     1081 atattccgca atagcttcag ctgttcttgc gaaccgtatg tgttccagcg cataagcata
     1141 gcgttcttca aaatagctca tgactaagtc actagcagct tgtgataaac caccttcacg
     1201 taattgggaa tgaaccaaac tccaaatata atctttgtca tatttggtga cctgatcaac
     1261 ttgacgggta aagtgaccct cagtcttttt cacactagtg cactcctttc tttcctcctc
     1321 gtcctcaatg actaaattac tctctttagt ttcactacct tcagtttcac tcttttcagt
     1381 ctcactaggg gctgaattta acacggggct tgtctgtttt tgagactggt cccgtgtttt
     1441 tttagactac ccccgtctga acttaagact ggggtggaat catgttccaa ctttcctaaa
     1501 taaatcttat tagccaaacg ccctttttca ctgggagact gctggccttc atcgatcaaa
     1561 ccatagtctc gtaggttttc ttaatcacta acaatttcga cttggaacag ccaaggagta
     1621 ctatcatttt gagttggaat aaatgagata gatggcaatc accatttgtc gtgatgactg
     1681 ttcattcatt tgcatgattt tcctcctgaa cataaaaaaa acgagaacac ttattaaaat
     1741 gttctcgttc acat
//
LOCUS       DAUCWU010000149         1661 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUCWU010000149 DAUCWU010000000
VERSION     DAUCWU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.151"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..743
                     /locus_tag="VGY24_001744"
     CDS             <1..743
                     /locus_tag="VGY24_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ4284693.1"
                     /translation="EGISLYYIGEYLAECLLIEHSDTLLIHAAAVYNPLSGHSILLLG
                     DKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYAGTSFFKIRRTAVLSDNLL
                     FKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTEISKIYYIKTDKKEKQIYH
                     SIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPLINYEKNNRVREQIVKGIF
                     AKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            798..>1661
                     /locus_tag="VGY24_001745"
     CDS             798..>1661
                     /locus_tag="VGY24_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ4284694.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDDYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAISHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FLEVSQSFFETINDNSLNQDKLRLNINFESHSRQFIKSSDILSSNDIENLKLRALFLL
                     QEEKQYPHGLSE"
BASE COUNT          615 a          201 c          299 g          546 t
ORIGIN      
        1 aagaaggaat ttcattatac tacattggag agtacttagc tgaatgtctc ttgattgaac
       61 atagtgacac cttattaatt catgcagcag cagtttataa tcctttatct ggccattcaa
      121 ttttactatt aggagataaa ggtgctggaa aaactacggt tgcaataagg ctttgtatag
      181 aacatgggta tcaccttatt ggtaatgacc aagtaatatt tggttcgaac tctggaatac
      241 tactaactta tgcagggaca tctttcttta aaataagaag gacagctgtt ttgtcggaca
      301 atttactgtt taaattattt agcaaatttt ttaatcgttc attacaattt aataaggcaa
      361 gttgggatga taaaatttca attttcccaa aggagttggg aatacgaact tgtaattttt
      421 ctactgaaat ctcaaaaatt tattatataa agacagacaa gaaagaaaag caaatttatc
      481 attcaatttg gagtgatagt ttagcaagtc tatatctaaa tgagctattg ggaaggcata
      541 tttctggaca ggttgcatgt tttcaatcag ataatggaaa atatttttct acaatgcctt
      601 tgataaatta tgaaaaaaat aatcgggtac gagaacagat agtgaagggt attttcgcta
      661 aacaaaaatt atttaaaatt acagcagcta cgccagctaa gattgcagaa gcgattatag
      721 aaaatgttaa gaaaaactca tagtgtgtgt gaatttgggg attaatatga agtattgaaa
      781 aaggagtaag agtaattatg tttgtgatag gcattccaac tttaaatgag gcagataata
      841 tcagtagatt agttaaacaa attgatgact atgctgtaaa tttagggaaa gaaattatta
      901 ttattaattc ggatagtaaa agtacagatg gaactccgca gattttttta gaaaccaaaa
      961 cttataacac caaagtttcg atagtatcag aagctaaagg aaaaggttat aatgtgagaa
     1021 atattttcga atatgcaata agtcatgtgc cgaatttctc cggtttaatt ttgattgatg
     1081 gagacgttgt ctcaatgaaa aaaatgtggt tagaaaagat gtttatagca attgaatctg
     1141 gaaatgatct tataatccca aattatgcta gaaaatcttt tgaaggaaat gcgacaaacc
     1201 attttattta tcccatgtta gtgaaatttt tcaaacgtga tatgccttat caatgcatct
     1261 ctggtgattt tggattttca agaggattga taaaagattt aactttgaag tgtaattggc
     1321 acaaatatac attaggttac gggatagata tttttttaac cttaacagct atattaaagt
     1381 catacaaaat taaggaaatc gatttacaat caaagattca taaaaaaagc tttgagaaaa
     1441 ttgaaaaaat ttttttagaa gtttcacaaa gtttcttcga aactattaat gataattctt
     1501 tgaatcaaga taagttacgt ttaaatataa attttgaaag tcattcaaga cagtttatta
     1561 agtccagtga tatcctatcc agcaatgata tcgagaattt aaagttaaga gcgttgtttt
     1621 tattacagga agaaaaacag tatccacatg ggctgtcaga g
//
LOCUS       DAUCWU010000150         1634 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUCWU010000150 DAUCWU010000000
VERSION     DAUCWU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.152"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            107..631
                     /locus_tag="VGY24_001746"
     CDS             107..631
                     /locus_tag="VGY24_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEQ4284695.1"
                     /translation="MQLIREDFSLPFLKQLKQVLRKECASLPMDLKCLLGAHIKPLEQ
                     SIDRVEGLSEILRRSNPKMALCHTDIHNWNLMQRDEQLVLIDWEGLKLAPVKADLMFF
                     VDKPYYDVFMNIYLKLHKDFLINTDALLFYHIRRKLEDIWEFIEQLLYDNQEDKERNE
                     TIKVLDGELNNLVF"
BASE COUNT          631 a          211 c          307 g          485 t
ORIGIN      
        1 acttatttgc atatttgcac gaaaatatag ttatcgacga ctattctgat aatagcaact
       61 aatatagtca atgataactc aaaatttatt gatatagtag gtatatgtgc aactaattag
      121 ggaagatttt agcttgccat ttctgaaaca gctaaaacaa gtattgcgta aagaatgcgc
      181 cagtcttccc atggacttaa aatgcttgct tggggcacac ataaaacccc ttgaacaatc
      241 tattgacaga gttgaaggac tgtcggagat tctaagacga agtaatccta aaatggccct
      301 atgccataca gatattcata attggaactt gatgcaacgg gatgagcagt tagttctaat
      361 tgactgggaa ggcttgaaat tggctccagt aaaagctgat ctcatgtttt ttgttgataa
      421 gccatattat gatgtattta tgaacatata cctgaaatta cacaaagatt ttttaatcaa
      481 tacggatgct ttgttattct atcatattag acgtaaacta gaagatatat gggagtttat
      541 tgaacaactt ttatatgaca atcaagagga taaggaaaga aatgaaacta taaaagttct
      601 tgatggtgaa ctgaataact tagtgttttg aaaaaagaat aatgtattat taagctaaat
      661 aatattggag aagaaaatga ataaacaaga attatatcaa attgctttca gtataattaa
      721 tcataaaaag ttacggaact ttggaacttc agggcatgtg gagacggtgg tattgatgtc
      781 aaggaaaata agataatagg ctataaaagt gttgaaatta atggttttgg ggttttagaa
      841 gaaatcctaa aagccatttt tctatttaga taaatatctt aattataatt aaagaataaa
      901 ttgaggatca agaacctcaa tattatatag aaaatagcca cgaagcgatc ataccaaaag
      961 aaatcttcat gcaagtctaa gaagaaatgg atagacgagc taatatggta agtggcaaag
     1021 ggaaaagaag aatctattcc agtaaatacg ccctttcaag tattgtctac tgctctatat
     1081 gtggggatat ttacaggaga atagcatgga ataatagagg aaagaaaaaa acaagttatt
     1141 tatagtgtta aattaccaac actcttatta cttcctatgt aatgtttaca gttctagtat
     1201 cataacttgt tcaattagag gatttaaaca gatttttgtg ggctgttttt tagtaccata
     1261 aatagtgtaa gaaaggaagt aattcctatc ttgcactatt tttgtataat gaagaagtag
     1321 tatgacgagg ctgttttagg gttgaaacat ccgccacaaa aactgtcaac taccttttca
     1381 ttattcatat tcggttaagc aggttgggca aaacgcccgt gtcacaagct aaaaagagtg
     1441 taatggtgca ggtagaaact acaaaataaa aagaaagagg aaaaaacatg atatctgtag
     1501 gaatagacat atcaaaagaa aaaatcacag tttgtgcatt aggacaagga agcaaaataa
     1561 tctggaaacc ctttgatgtt tctctaaaaa aacagaagta gaaaaatttt tagagaaact
     1621 agaaaaaata aaag
//
LOCUS       DAUCWU010000151         1601 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUCWU010000151 DAUCWU010000000
VERSION     DAUCWU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.153"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            106..1587
                     /gene="guaB"
                     /locus_tag="VGY24_001747"
     CDS             106..1587
                     /gene="guaB"
                     /locus_tag="VGY24_001747"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ4284696.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          451 a          278 c          370 g          502 t
ORIGIN      
        1 taggaatatt ttattgacaa atgattttag cgtgatatga tagtaacaat aaaaaataga
       61 ccagggtaat attccctaaa aataaaagaa aagaggatta tctggatgtc aaattgggac
      121 actaaatttt tgaaaaaagg ctacactttt gacgatgtct tgcttattcc ggcagagagt
      181 catgttcttc caaatgaagt tgatttaaag acaaagctcg cagacaattt gacattaaat
      241 attcctatta ttacagcagc tatggatacg gttactggta gtaaaatggc tattgcaatt
      301 gcgcgtgcgg gtggtttagg tgttattcat aaaaacatgt ctattactga gcaagcagaa
      361 gaagttcgca aggtaaaacg ttctgaaaat ggtgtcatca ttgatccatt cttcttaaca
      421 cctgaacaca aggtttcaga agctgaagaa ttaatgcagc gctaccgtat cagtggtgtg
      481 ccaattgttg aaactttagc aaaccgtaag ttggtcggta tcattactaa tcgtgatatg
      541 cgttttattt ctgattataa tgcaccaatc tctgaacata tgactagtga gcacctcgtt
      601 acggctgctg ttggtacaga tcttgaaact gcagaacgta ttcttcatga acaccgtatt
      661 gaaaaacttc ctttggttga taatagcggt cgtctgtctg gtttaatcac tatcaaagat
      721 attgaaaaag taatcgaatt cccacatgct gctaaagatg aatttggtcg tcttctggtc
      781 gctgctgcgg taggtgttac ctcagatact tttgagcgtg ccgaggcctt gtttgaagct
      841 ggtgcagatg cgattgttat tgacacagct catggtcatt cggctggtgt tcttcgtaaa
      901 attgctgaga ttcgtgctca tttcccaaat cgtactttga ttgctggaaa tattgccact
      961 gctgaaggtg cgcgtgctct ttatgatgca ggtgttgatg ttgttaaagt gggtattggt
     1021 cccggatcta tctgtaccac tcgtgtggtt gcaggtgtcg gtgttcctca agtgactgct
     1081 atttacgatg ctgctgctgt tgctcgtgaa tatggcaaaa cgattattgc tgatggtggt
     1141 atcaagtatt ctggcgatat tgttaaggct cttgcagccg gtggtaatgc tgttatgctt
     1201 ggatcaatgt ttgcaggtac tgatgaagct ccaggtgaaa ctgaaatcta ccaaggccgt
     1261 aagttcaaaa cttatcgtgg aatgggttcc attgcagcta tgaagaaagg atcaagcgat
     1321 cgttacttcc aaggttccgt caatgaagct aataaactag ttccagaagg tattgaaggc
     1381 cgtgttgctt ataaaggagc tgcttcagat attgtcttcc aaatgttagg tggtatccgc
     1441 tcaggtatgg gctatgttgg cgcaggtgac attcaagaat tacatgaaaa tgctcaattt
     1501 gttgaaatgt caggtgcagg cttgattgaa agtcatccac atgatgtcca aatcacaaat
     1561 gaagcaccaa actattctgt gcactgataa tttaatttag t
//
LOCUS       DAUCWU010000152         1553 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUCWU010000152 DAUCWU010000000
VERSION     DAUCWU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.154"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            92..850
                     /gene="mf2"
                     /locus_tag="VGY24_001748"
     CDS             92..850
                     /gene="mf2"
                     /locus_tag="VGY24_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ4284697.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
     gene            961..>1553
                     /gene="speC"
                     /locus_tag="VGY24_001749"
     CDS             961..>1553
                     /gene="speC"
                     /locus_tag="VGY24_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ4284698.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEID
                     FKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKHEQI"
BASE COUNT          571 a          252 c          244 g          486 t
ORIGIN      
        1 gtttttttaa tttaaagtga tataattaac tagaaaagtg caatatttga tccactctaa
       61 aagctaaaaa tggaaaggtt taggtgttat catgaaatta tctaaacaaa aggcaagttt
      121 gcttaccgct gttttactgt tactttctct ttctataacg acaataactg ttgacgcagc
      181 taaggtacga acttatccga atgtatccca tgcaaacaca cattataaaa atactgcttc
      241 tagtaagctc ttacctttta ctgctaatta tcagttacaa cttggtgagt tggataacct
      301 taaccgcgct accttttcac atatccaact gcaagataga cacgaaacta aagatgtacg
      361 cactaaaata aattatgacc ctgtgggatg gcataactat caatttccat acggagatgg
      421 ctcaaaatct tcatgggtaa tgaatcgtgg gcatctagtc ggatatcaat tttgtggatt
      481 aaacgatgaa ccaagaaatt tagtgacaat gacagcctgg ctaaacacag gtgcttattc
      541 aggagcaaat gacagcaatc ctgaagggat gttatactat gaaaaccgtt tagattcttg
      601 gcttgcactt caccctgact tttggctaga ttataaagtc actcctatat atagtggcaa
      661 tgaagtagtg cctcgacaaa ttgaattaca gtatgttgga attgattcat ctggtgagtt
      721 gcttactata aggctaaata gtaataaaga aagtattgat gaaaatggtg ttactacagt
      781 aatcttagaa aactctgctc caaatatcaa ccttgactat ttaaatggaa ctgccactcc
      841 taaaaactaa aatataaata catttataaa atttctaaat aaacagaaat ctgattttta
      901 actacttact gctatttcat gtattctcgt acgagtaata catttaatta aggagaaaaa
      961 atgaaaaaga ttaacatcat caaaatagtt ttcataatta cagtcatact gatttctact
     1021 atttcaccta tcatcaaaag tgactctaag aaagacattt cgaatgttaa aagtgattta
     1081 ctttatgcat acactataac tccttatgat tataaagatt gcagggtaaa tttttcaacg
     1141 acacacacat taaacattga tactcaaaaa tatagaggga aagactatta tattagttcc
     1201 gaaatgtctt atgaggcctc tcaaaaattt aaacgagatg atcatgtaga tgtttttgga
     1261 ttattttata ttcttaattc tcacaccggt gagtacatct atggaggaat tacacctgct
     1321 caaaataata aagtaaatca taaattattg ggaaatctat ttatttcggg agaatctcaa
     1381 cagaacttaa ataacaaaat tattctagaa aaagatatcg taactttcca ggaaattgac
     1441 tttaaaatca gaaaatacct tatggataat tataaaattt atgacgctac ttctccttat
     1501 gtaagcggca gaatcgaaat tggcacaaaa gatgggaaac atgagcaaat aga
//
LOCUS       DAUCWU010000153         1535 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.155, whole genome shotgun sequence.
ACCESSION   DAUCWU010000153 DAUCWU010000000
VERSION     DAUCWU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.155"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..593
                     /locus_tag="VGY24_001750"
     CDS             <1..593
                     /locus_tag="VGY24_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284699.1"
                     /translation="QINISPKNMLNMDNPSTPTTPKVELIPSTLQKINSTSSQLEHSR
                     QIIVLDTAETLLEQQKEIKNNEDTIAELFSYNYYDHAASAGTGQYLNDVQVETIELPV
                     DYDADFVIPVYGDSMEPKYHSGDYVFVKLSIDLSDGDIGVFEYYGDAYIKQLLINAEG
                     AFLHSLNSKYDDIPIDKDSDFRIIGEVVGSFTPVEM"
     gene            778..>1535
                     /locus_tag="VGY24_001751"
     CDS             778..>1535
                     /locus_tag="VGY24_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019769798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEQ4284700.1"
                     /translation="MKEYKTFNQQMMILRGRGMTVPTDGRPKRFLEQENYYNVINGYK
                     DLFLKRDANGRAIVPEEYLDGTHFNELKKLFLFDRELRILFLKYLLIFENSIKTTIAH
                     TFSKKYPRKNSYLDLSNFVDDNPKQVLNQISILTKTIHDKVGQHGSVKHYIEGHGEIP
                     LWVLVNYLTIGNISHFYSILTQPLKEEITKFYSNKYNKQIYTDNELRLSPEDLGAALK
                     ATNLIRNACAHDERLYNISLKNIRIRNLSQFYGI"
BASE COUNT          544 a          274 c          267 g          450 t
ORIGIN      
        1 tacagattaa tatttctcct aagaatatgt taaatatgga taatccctcc actcccacca
       61 ccccaaaagt cgaacttatc ccatctactc tacaaaaaat aaactctact tcttctcaac
      121 tagaacacag tagacagata attgttttag atacagctga gactttattg gaacaacaga
      181 aagaaattaa aaacaacgaa gatactattg ccgaattatt ttcttacaac tactacgacc
      241 acgcagcttc agcaggtaca ggtcagtatc taaatgatgt gcaagtggaa acaattgaac
      301 taccagtcga ttatgacgca gactttgtta tccccgtcta tggtgattct atggagccta
      361 agtaccattc tggggactat gtatttgtca agctgtccat agacctctca gacggcgata
      421 taggcgtgtt tgagtattac ggtgacgctt atatcaaaca gttgcttata aacgctgagg
      481 gggcatttct gcatagcttg aacagcaagt atgacgatat accgatagat aaagatagtg
      541 actttagaat tattggtgaa gtcgtcggca gttttacacc cgtagaaatg taattgaata
      601 gatagcgtta acgacgattt ttgccaaaag taaaattgaa tagctattga tgaaaaatga
      661 aatgaacgtt tttgttgact ttatcgcaca atagttgtac tatatgtaca taggattagg
      721 tcaggaagcg tacggcgccc tggccatttt tgtttttata aaaaatggag caacaacatg
      781 aaagaatata aaacttttaa tcaacaaatg atgattttaa gagggcgagg gatgactgtt
      841 cccactgacg ggagacctaa acgttttcta gaacaagaaa attactataa tgttatcaat
      901 ggatacaaag atttattctt aaagcgagac gccaatggta gagctattgt tccagaagaa
      961 tatctagatg gaactcattt taacgaatta aagaaattat ttttgttcga tagagagcta
     1021 cgcatattat ttttaaagta tttattaata ttcgaaaact caataaaaac aactatcgct
     1081 cacacatttt ctaaaaaata cccacgtaaa aactcttacc tagatttaag taactttgta
     1141 gatgataatc ctaaacaagt cctcaatcag atttcaatac tcaccaagac tattcatgac
     1201 aaagttggac agcatggttc tgttaagcac tatatcgaag ggcatggaga gattcctctg
     1261 tgggtgcttg ttaattactt aaccatagga aacatttctc atttttacag cattcttact
     1321 caaccgctga aagaagagat aacaaaattt tacagcaata aatataataa acaaatttac
     1381 acagataacg aactacgctt gtctccagaa gatttaggag cggcattaaa agcgactaat
     1441 cttatcagaa atgcttgcgc acacgatgaa agactgtaca atattagctt gaaaaatatt
     1501 agaattcgca acctatctca attttatggt atcca
//
LOCUS       DAUCWU010000154         1474 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.156, whole genome shotgun sequence.
ACCESSION   DAUCWU010000154 DAUCWU010000000
VERSION     DAUCWU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.156"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(113..1447)
                     /locus_tag="VGY24_001752"
     CDS             complement(113..1447)
                     /locus_tag="VGY24_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ4284701.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
BASE COUNT          420 a          308 c          247 g          499 t
ORIGIN      
        1 gaactttagt catttcgata tttatcttga aaaataattc atcatacaca aaaaaccgcc
       61 cagaaattat ctgagcggtt ttgtcttatc taaaggagct ttacctccta ttttatactt
      121 gtgttgcatt agataaatac ttatcttcga cccactggtc agactgagaa gcgttaatac
      181 gtgaccatcc attcactttt tcatagactc ttacacgagt tcctgccttg ataaattctt
      241 tatcagcgct acttgcgttt ggcttagact ctacataata gtctgttcta agggttgctt
      301 cgtagtaagg tacattcgag ttgtctaatt tagtattagt atctagcttt tgattaaaag
      361 taagctggct ttgtggtttg tcaatcttag gtatatccac tttgctacta tcatctgcta
      421 ataatacaat atttttatct aaaccacctg ctactcctac acttgtgaac tgccaccagc
      481 gcacaccatc cattgaaggg aagaactccc aaagtggctc tgttcgtact tcgtagtctg
      541 gataaccagc tatccaaatg ctgtttgggt acttagcgat aatttgctga taatcaatat
      601 tattaagcgt aaatggttta tagctgtaat aaacaggctt atatccagcg tttgcaattt
      661 tatccataaa cgcaataact gcattagtgt tagcttcttt gtcggcactt gcagagtctt
      721 cataatcaat gactaagtaa gagacttttt tggttggtaa attggacaga aataaatctg
      781 cttctcgttg cgctaagttg ctatctcctc caaatcgtcc aaagtgataa taaccaatag
      841 ggtcactagt attagcttgt tgctgatgcc tatcagacag ccaagcgagt gactcggata
      901 ctttgataat cgttttagta gtgccagctt gctgacaagt agcggttaag tctgcttgtt
      961 gataagctga tacatcaata aaataatcgc ctttatttag tcctatatta cctataacag
     1021 taactgcatt tttaaaaact tttggtctaa atgcagtagg ataagtcgtt gaatatggta
     1081 tttttactaa attatatgct ccgtttgctc caccctggtt ttgacctaaa aaccatccgt
     1141 atccactacc tgcatcacta tcaaaaatag ccacatggct atacggtgta acacctgcaa
     1201 ctaccataaa aattgcgaca tctccagctt gcatagtctc tacttcgtca aagtagttta
     1261 aaataccatt tttgtgacgt tgttcccata tatcccttgc atatcctgta tttgtacagt
     1321 ttgcgtatgg cagtcctaga tacttacagt agtctgcgta gccatcccaa cattgcgcac
     1381 caagcgatcc atcaatatcg taagcattgc catttgaacg acttttatat tcctgatatg
     1441 tagccattta tccctcctta tctaaaaatg gata
//
LOCUS       DAUCWU010000155         1471 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.157, whole genome shotgun sequence.
ACCESSION   DAUCWU010000155 DAUCWU010000000
VERSION     DAUCWU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.157"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1400)
                     /locus_tag="VGY24_001753"
     CDS             complement(<1..1400)
                     /locus_tag="VGY24_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEQ4284702.1"
                     /translation="MKLVIAEKPSVAVTIAKVIGARTRKNGYYEGNGYIVSWCVGHLI
                     QMASPDKIDEKWKKWTIDTLPIIPEDYIYEVSKSTKKQYGILKKILNDKNIDTVINAC
                     DAGREGELIFRLVYNQAKCKKKIQRLWISSMENKAIEDGFRNIKDGENFEDLYRSASA
                     RAIADWLVGMNLSRLYSCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLS
                     YEGLKLVSDRIDRIEVAEQLLNLLEDEIVITEVEDKDISTKPDKPYDLTTLQREANKY
                     FGYSANDTLNLAQGLYEKKLITYPRTDSRYLTDDMVNTMKELLEGLEDDFKINESNFK
                     SIFNSSNVTDHYAIIPTISGIGKAKDLSDRESKIYNLIKNKLLASCSDNLKESSRKIR
                     YEYDKFNFNASGKTIIDEGYTKYLKTYGKEKQENELPDVKTGDKIKLTSKKISEKFTK
                     APSHYNEDTLLKAMENA"
BASE COUNT          411 a          275 c          178 g          607 t
ORIGIN      
        1 gcattttcca tagcctttaa aagtgtatct tcattataat gacttggagc ttttgtaaat
       61 ttctccgata tttttttaga agttagctta attttatctc cagtctttac atctggtaat
      121 tcattttctt gtttttcctt tccataggtc tttagatact ttgtataacc ttcatcgatt
      181 atagtcttgc cacttgcatt aaagttaaac ttgtcatatt catatctgat ttttctacta
      241 gattccttta agttatccga acatgaagca agtaatttat tctttattag attatagatt
      301 ttactttctc tatcagataa atctttggct tttccaatac ctgatatagt aggaataatc
      361 gcatagtggt ctgtaacatt agatgaatta aaaatagact taaaatttga ttcattgatt
      421 ttaaagtcat cttcaagtcc ttctaataat tctttcatag tattaaccat atcatcggtt
      481 aaatacctac tatctgttct tgggtatgtt attagctttt tttcatacag accttgtgcc
      541 agatttaaag tgtcatttgc tgaatatcca aaatatttat ttgcttctct ttgtaaggta
      601 gtgagatcat aaggcttatc tggttttgtg cttatatctt tatcttctac ctctgtaata
      661 actatttcat cttctaacag atttaatagt tgttctgcaa cttcaattct atcaatccta
      721 tctgatacaa gcttcaatcc ttcataagat aggtcaactg tataatactt ttgcttctta
      781 aataggttta tttcactatc ccttttagct attaaatata gagttggtgt ttgtactcta
      841 ccaacagaat atgtttcctt gtaaatgcaa gaataaagcc tacttaaatt cattcctacc
      901 agccaatccg caattgctct tgcacttgct gatctatata agtcttcaaa gttttctccg
      961 tctttaatat ttctaaagcc atcttcaata gctttgtttt ccattgaaga tatccaaagt
     1021 ctttgaatct tcttcttaca tttagcttga ttatatacaa gcctaaaaat aagttctccc
     1081 tctcttccag catcacaagc atttattact gtgtcgatgt tcttatcgtt taaaattttc
     1141 tttaaaattc catattgttt tttagtgctt ttagatactt cataaatata gtcctctggg
     1201 attataggaa gagtgtctat tgtccatttc ttccattttt cgtctatctt atcgggactt
     1261 gccatttgaa ttaaatgacc tacacaccaa gaaacaatgt atccattccc ctcataatat
     1321 ccgttttttc ttgttcttgc tccaattact tttgcaattg taactgctac acttggcttt
     1381 tctgctatta ctaacttcat taatacctcc ataaataaaa aaaagcgaaa gatttctctc
     1441 tcgctcaaag tttttctccc gaatattgta t
//
LOCUS       DAUCWU010000156         1400 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.158, whole genome shotgun sequence.
ACCESSION   DAUCWU010000156 DAUCWU010000000
VERSION     DAUCWU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.158"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..661)
                     /locus_tag="VGY24_001754"
     CDS             complement(<1..661)
                     /locus_tag="VGY24_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284703.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNQFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YA"
     gene            complement(664..1389)
                     /locus_tag="VGY24_001755"
     CDS             complement(664..1389)
                     /locus_tag="VGY24_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284704.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEVFGDRSASLNDIYLALTKED"
BASE COUNT          472 a          256 c          236 g          436 t
ORIGIN      
        1 tagcataaag ggacagccaa aggtttgaag gagtttgctt aactaatttt aaaagaggat
       61 ttagataagt tctttctttc acttttgtag aaattccttt gactgttgta aaaagagaat
      121 aaaactttaa aatactttgc ttgcgattgc tttcaaatgc gacagctttt gcccaatcta
      181 aacgatttcc tctcaaaaaa tatcttgtct ttcgactaag cactactctt tttagtgcca
      241 ataatatcaa aactaagata ataaaaataa ataaagacag acctagacgt ctaaaaatag
      301 gatataaaaa gagcaataca gcagtctgca aagttcccca aagcaaaaaa gagagtcgct
      361 cagcttgttt gatataagaa atcacttctt cttcttttgg aagtaagaaa tgctgatctg
      421 cctcttctag atacgttgct atagatccca tactgagcaa taatgggatc aatatcccaa
      481 gacaaacctg aataggtaga tgattcgttg gaaactggtt tagtagctga ccatactgca
      541 ccatagcaaa gcctaataaa aacatcaaca ccaaaacaaa gtgatcattt aagacgtaac
      601 gaaggtattt gttttgttgt ttttgaaagt cttgacggcg tttcagaaat aatgctttca
      661 tacttaatcc tcttttgtta atgcaagata gatatcattt aagctcgctg aacggtcacc
      721 aaaaacttct tgcaagtcag caagagttcc ttgggcacga acttgtccat gatgcaaaat
      781 cacaaagcga tcacacattc tttcagcaga gtccaaaaca tgtgtactca tcaaaataga
      841 ttttcctttt gctttttcga cttctagcgt ttggattaag tctgaaatag ctaaagggtc
      901 taaccctaag aatggttcat caagaataaa tagacttgga tcaatgacaa aggcacaaat
      961 aatcatgact ttttgtttca tccctttaga aaaattgaca ggaaaccatt caagtttgtc
     1021 agttaaacga aacatttcca agaaaggttg tgctcgtttt tgtgccactt ctaagtcgat
     1081 atcataagcc atagctacgg tgttgatatg ctctgataag gtcaattctt catagagact
     1141 aggagtttct gggataaaac ctattttttg acggtaggca actgcatttt ctgctagagt
     1201 taaaccatca atagaaatac ttccttgata aggcttcaaa aagccaataa tttcgttgat
     1261 agttgttgac tttccagcac catttagccc gatcaaaccg actaactctc cattgtctac
     1321 agaaaatgag acatcgttta aaacagggat attgtgatat cctcctgtta gatttttaat
     1381 gtttaacata taatttcttt
//
LOCUS       DAUCWU010000157         1379 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.159, whole genome shotgun sequence.
ACCESSION   DAUCWU010000157 DAUCWU010000000
VERSION     DAUCWU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.159"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>1379
                     /locus_tag="VGY24_001756"
     CDS             <1..>1379
                     /locus_tag="VGY24_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284705.1"
                     /translation="FNMPLGDILNESTGKLKDIIVNQVDNTETTLAHMIPEMTANLVG
                     PIILFVYMLILDYRLSLISLIPLVIGGFFMAGPMKRMKVKFPQAVKIGQDMNNSVVEY
                     INGIEVIKTFNQGEKSYRKYRDNVYKKANYYYDWMGENTRDYAISMSIAPVGILTIIP
                     FGLYFCMNGSLDGGVFLTLIILSFGTIQNIMRVMTFEDDIGRMSTIFEEIKNILSARE
                     LSHKNANLDIKNHNIEMKNVDFSYEKDKQVLSNINLNIEEGSVNALVGESGSGKSTIA
                     KLISGFWDVDSGSISIGNVNIKDMSLEKLSTIISYVAQDNFLFDMSIKDNIRIGNKNA
                     SDEEIIEVCKKAACHDFIMKLSDGYDTRVGEAGKHLSGGERQRISIARAMIKNAPIVI
                     LDEATSYIDPENEALIQDAISELVKGKTLIIIAHRLKTITDVDNIFVIKNGELACKGS
                     HEELLKESK"
BASE COUNT          527 a          154 c          268 g          430 t
ORIGIN      
        1 tttaatatgc cattaggaga tatattgaat gaatctactg gtaagttaaa agacataatt
       61 gtaaatcaag tcgataatac tgaaacaaca ttggctcata tgatacctga gatgacagct
      121 aatttagtcg gacctataat attatttgtt tacatgttaa ttttagatta tagactaagt
      181 ttaatatctt taattccatt agttattggt ggatttttta tggcagggcc gatgaaaaga
      241 atgaaggtaa agtttccaca agcagttaaa attggccaag atatgaacaa ttctgtagtt
      301 gaatatataa atggaataga ggttattaaa acatttaatc aaggagaaaa atcttataga
      361 aaatacaggg acaatgtgta taaaaaggca aattactatt acgattggat gggagaaaat
      421 acaagagact atgcgataag tatgtctatt gctccagttg gtattttgac aattatacca
      481 tttggtttat atttctgtat gaatggttca ctagatggtg gtgtattttt aacattgata
      541 attctttctt ttgggactat ccaaaatatt atgagagtaa tgacttttga agatgatatt
      601 ggaagaatgt caactatttt cgaggagatt aaaaatatac tttctgcgag agaactatct
      661 cataaaaatg caaatttaga tataaaaaat cataatatag agatgaaaaa cgtagatttt
      721 tcttatgaaa aagacaagca agtcctaagc aatataaatt taaacataga agaaggaagt
      781 gtgaatgctt tagtaggtga atcgggttcg ggtaagtcga ctattgcaaa actaatttca
      841 ggattttggg acgtagatag tggttctata agtataggaa atgtaaatat taaagatatg
      901 tcccttgaaa agctatctac tattatttcc tatgtagcac aagataattt tctttttgat
      961 atgtcaatta aagataacat cagaatagga aataaaaatg ctagtgatga ggaaataatt
     1021 gaagtatgta agaaagcagc ctgccatgat tttataatga agctgtcaga tggatatgat
     1081 acgagagtag gagaagccgg taaacattta tctggaggag aaaggcaaag aatatcaatt
     1141 gcaagagcta tgataaagaa tgctccaata gttattttag acgaggctac ctcttatatc
     1201 gatcctgaaa atgaagcctt gattcaagat gcaatatcag aactggtcaa agggaaaact
     1261 ctaattataa ttgcccatcg tttaaagaca ataacagatg tagataatat atttgtaatt
     1321 aaaaatggag aacttgcatg taaaggaagt catgaagagc ttttgaagga atcaaaaga
//
LOCUS       DAUCWU010000158         1365 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.160, whole genome shotgun sequence.
ACCESSION   DAUCWU010000158 DAUCWU010000000
VERSION     DAUCWU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.160"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            64..151
                     /locus_tag="VGY24_001757"
     tRNA            64..151
                     /locus_tag="VGY24_001757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Ser,seq:gct)
     gene            complement(627..908)
                     /locus_tag="VGY24_001758"
     CDS             complement(627..908)
                     /locus_tag="VGY24_001758"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ4284706.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAIIRFRIRIINTSAIKPIAYRTLKLY"
BASE COUNT          453 a          163 c          285 g          464 t
ORIGIN      
        1 tagagcgcac gcctgataag cgtgaggtcg gtggttcgag tccactcgtg cccatatata
       61 tttggagaat tactcaagag gctgaagagg acggtttgct aaatcgttag gtcgggtaac
      121 tggcgcaagg gttcgaatcc cttattctcc gttttgtaaa gatattttgg ttaatatgtt
      181 aaatgaaaca tattaaagga tgaatcataa ataaagggga aagaagacgg ttatcgtctt
      241 tttttgtaat cttcaaaacc aactaaatag aatatttaaa tcgttcatta tttcgataac
      301 taaaatgata ggctctttga ctactgtaga ggagtaaaac gtttaacttg atggctttga
      361 taaggttgtt gactgtttct aatttggcct tagagtagtt gagtttaagt atattgctaa
      421 tcttgttttc ttttctttta gaaaaatttt aaaaattgtc tgaaaaatgg gaagagcaag
      481 agttgataga gttaggaata attattgaaa ttcagagatc ttgtgagtaa gagcttttct
      541 tttctagatg ttagttatgg gcgtacgaaa aattgggcaa taaagctatt attgatgagc
      601 ttccgtgaat catgctgtat gagttttcag taaagtttga gagttctata cgcgataggt
      661 tttatagcag aagtatttat gattcggata cgaaagcgaa ttatagcttg tctaagacgt
      721 tgggggatat gaaaacaatc gaggataatt ttagcctgta gaaaaagcat agtaatctca
      781 tagtagtggg cactgagtat gcctatggta ataactttga catgccttct aacctgtctg
      841 gtataatgaa agagatgatt atgaatgctt tttgcgtccc cagcatttac ataatttaaa
      901 accgtgatgc taaggtttta gattcctaat gaaattatac tataatcgga ttattctata
      961 tgagtctttc tgaaatgata gtttggtcgc ttttcattat agatcgtcat atggaacttt
     1021 ttacatatgt ttaaaaagac tctaaaatct ttgaggtgga tttacctact acagaaagta
     1081 tagaattaaa agttgtgaaa gtttgcgagt ctttatgaat tgtgattgag tgatggaata
     1141 ctaagattta tagatgacca gttttcgata gttagcttat aaaaaaacaa ggtctgacta
     1201 tacttatatt aataatgata aggtcaaaaa agatgtctaa tattctaaat agaaaatgtc
     1261 actattgtgg ataatgtttt aggcgaaaag aagttattaa cctgtgaata agtaactaaa
     1321 aattcattct aagaaggata aggtgtctga gtgaagataa acaat
//
LOCUS       DAUCWU010000159         1304 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.161, whole genome shotgun sequence.
ACCESSION   DAUCWU010000159 DAUCWU010000000
VERSION     DAUCWU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.161"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            11..1207
                     /locus_tag="VGY24_001759"
     CDS             11..1207
                     /locus_tag="VGY24_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ4284707.1"
                     /translation="MKKRILSAVLVSGVTLGAATVVSADNFDAKISDANSRISALTAE
                     QQSAQNQVNALQAQVSSLQAEQDKLTAKNSELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT          425 a          249 c          265 g          365 t
ORIGIN      
        1 tttttttatt atgaaaaaaa gaattctatc agccgttctt gtaagtggtg taacccttgg
       61 agcagctact gtagttagcg cggataattt tgatgccaaa atctctgatg ctaactcaag
      121 aatctcagcc ttaactgcag aacagcaatc tgctcagaat caagttaatg cattacaggc
      181 acaagttagt tcattacaag ctgaacaaga taaattaaca gcaaaaaatt cagaacttga
      241 ggcgctttca aagcgatttg agcaagaaat taaggctcta acaagtcaaa ttgttgctcg
      301 taatgaaaaa ttaaaaaatc aagctcgtag tgcttataaa aacaatgaaa cttctggtta
      361 tattaatgca cttttgaatt ctaaatcaat ttctgatgtt gtaaaccgtt tagtagcaat
      421 taatagagct gtctctgcta acgctaaatt gttagaacaa caaaaagctg ataaagtttc
      481 ccttgaagaa aagcaagctg ctaaccaaac agctattaat accattgccg ctaatatggc
      541 aatggctgaa gaaaaccaaa atacattacg tactcaacaa gctaatttgg tagctgcaac
      601 tgcaaattta gctctccaat tagcatctgc tactgaagat aaagctaatt tggtagctca
      661 aaaagaagct gcagaaaaag ctgctgctga agccttagca caagaacagg ctgctaaagt
      721 taaggcacaa gaacaggctg cacaacaagc agcatctgtt gaagcagcaa aatctgctat
      781 tactccagca ccacaagcta ctccggcagc gcaaagtagt aatgctattg aaccagctgc
      841 actcacggct ccggcagctc cttctgcagg accacaaaca tcatatgatt cttctaatac
      901 ttatccagtt ggacaatgca catggggagc taaatcttta gctccttggg caggaaataa
      961 ttggggaaat ggtggtcaat gggcttatag tgctcaagca gctggttatc gtactggttc
     1021 aacgccgatg gtaggtgcga ttgccgtttg gaacgatggt ggttatggac atgtcgccgt
     1081 tgtagttgag gttcaaagtg cctcaagtat tcgtgtgatg gagtctaact acagtggtag
     1141 acagtacatt gctgaccacc gtggctggtt taatccaaca ggtgttacat ttatttatcc
     1201 acactaacat attatttagg ttagaacagt tgttctaacc tttttgatta ctcctattgt
     1261 ggaatagttc tgagagtatt gacgatatag ttaatttata agag
//
LOCUS       DAUCWU010000160         1284 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.162, whole genome shotgun sequence.
ACCESSION   DAUCWU010000160 DAUCWU010000000
VERSION     DAUCWU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.162"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            376..492
                     /locus_tag="VGY24_001760"
                     /pseudo
     CDS             376..492
                     /locus_tag="VGY24_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(678..>1284)
                     /locus_tag="VGY24_001761"
     CDS             complement(678..>1284)
                     /locus_tag="VGY24_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ4284708.1"
                     /translation="VAKETKALISGDRYLSETEKAVQKQAVEQALAKALGQVEAAKTV
                     EAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNEAKQAAIEALKQAAAETLAKITTD
                     AKLTEAQKAEQSENVSLALKTAIATVRSAQSIASVKEAKDKGITAIRAAYVPNKAVAK
                     SSSANHLPKSGDANSIVLVGLGVMSLLLGMVLYSKKKESKD"
BASE COUNT          407 a          249 c          209 g          419 t
ORIGIN      
        1 tttttaatat tatcaaaaat gacaaaaaaa taataattta ttaatatata gacaattatg
       61 atctaatata ctgaatttta taatagattc tatcaataac tatgtgtaaa caatcaagta
      121 gaaatgtctt tattaagcga atcaatcaag gttgtagagt aaatactgga ctagatttca
      181 tgtagaaatt agtaaaagtt aggagatttt ttggtcgttt caaacgataa catcatcttc
      241 ttataacaag aacttcactc agagataaac tattctaata ctttagtagc ctcttttgag
      301 cttgtagcac gaaaattcgc tcgaactgat ttaaaatagg cattttatct gctcgcttga
      361 tccctactgc gcgaccaaga aaccttttat tcactagaac aattaaccct taaaaccaag
      421 gaggaagtcc actggtggaa ctaccatcac cttcacagta atcttaatta tcaaacaccc
      481 ataactaaat gagtcatcgt ttgaacaaaa aacgccttat aaattctgta cagaaaactg
      541 ttgccttttc aattgaactt ttaaatgttt tcaatacact ccgttcttgg gcccttacga
      601 tccaaaaaac caaggtgatt tcaaaatcac cttggtttga aaatatagta gaaagcaatt
      661 tggataaagc caggttctta gtctttactt tcttttttct tgctataaag caccatacct
      721 aaaagaagag acataactcc taagccaaca agaacaattg agtttgcatc acctgatttt
      781 ggaagatggt tcgctgacga tgattttgcg actgccttat taggcacata ggctgcacgg
      841 atagcagtaa tacctttatc ttttgcttct ttcacagacg caatagattg tgctgaacga
      901 acagtcgcaa tagccgtctt aagcgctaat gatacatttt ctgattgttc agctttttga
      961 gcttcagtca atttagcatc agttgtaatc ttagcaagtg tttctgccgc agcttgttta
     1021 agagcttcaa tagcagcttg tttcgcttcg ttaagagcag ctgttgcttg atcagtatct
     1081 ttagctaaac cagcaacata tgctgaacgg atagctacag taccaaggtt ttctgccaac
     1141 ttaacagctt caactgtctt agcagcctca acttgaccaa gtgctttcgc aagagcttgc
     1201 tcaacagctt gtttttggac tgctttttca gtttcgctca agtaacggtc tcctgaaata
     1261 agagctttag tttctttagc tact
//
LOCUS       DAUCWU010000161         1172 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.164, whole genome shotgun sequence.
ACCESSION   DAUCWU010000161 DAUCWU010000000
VERSION     DAUCWU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.164"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..918
                     /locus_tag="VGY24_001762"
     CDS             <1..918
                     /locus_tag="VGY24_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ4284709.1"
                     /translation="YMKNTNFAEDFFKKLNNDGITDPEVNKEMIYINKSGANIQNKVN
                     GTDYQLLDNKVATIYIPDKFKEKKKSIENTVVAEQFTGTKYTKENLAVQIIPNEKKIF
                     YFNENGNEHDENKEASPMANVTDSKGNIVVVLDTDKMVASKDFSLASNIVNSSLFSPE
                     AIKKINDLSLHLNFSINPVDVYQLVKLNIQSLKHQIFLSKVLQKIIYGIVFLLIYQYA
                     QLFISSKQNDYVKKIILGLSKTRIAISSLKYFIMTIAIVIICTFMVTEQIELLYIGIA
                     SLVVLILSTVMSFRKLSKKYTQILKGDEQ"
     gene            915..>1172
                     /locus_tag="VGY24_001763"
     CDS             915..>1172
                     /locus_tag="VGY24_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284710.1"
                     /translation="MTIEIFNVTKKFSSKKIFTDLNLTFEAGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDNGKVLVDEQDIWKIKERTFFKNIIGYVFQNY"
BASE COUNT          471 a          146 c          182 g          373 t
ORIGIN      
        1 tacatgaaaa atactaactt tgcagaagat tttttcaaaa aattaaataa tgatggtata
       61 acggatccag aagtaaataa agagatgatt tatatcaata aatcgggcgc taatattcaa
      121 aataaagtca atggaacaga ctatcagctg ttagataata aggtagcaac aatctatatt
      181 cctgataaat ttaaagagaa aaagaaatca attgaaaata ccgttgtggc agaacaattt
      241 actggaacta agtatacaaa agaaaacctt gcagtgcaga taattcctaa tgagaaaaaa
      301 attttttatt ttaatgagaa tggaaacgag catgatgaaa ataaagaagc ctcacctatg
      361 gcaaatgtaa cagatagtaa aggtaatata gtcgttgtgt tagatactga taaaatggta
      421 gcaagcaaag atttttcttt ggctagcaat atagtaaata gctctctttt tagtcctgaa
      481 gcaataaaaa aaattaatga cctgagtcta catttaaatt tttcaattaa tccagtagat
      541 gtttaccaac ttgtaaaact taatattcaa tccttaaagc accaaatttt cctttcgaaa
      601 gttttgcaga aaattattta cggcattgta tttctactaa tttatcaata tgctcaatta
      661 tttatttctt caaaacagaa tgactatgtg aaaaaaataa ttttaggcct ctcaaaaaca
      721 cgcatagcaa tttcgagtct caaatatttt attatgacta ttgcaatagt tataatatgt
      781 acatttatgg tgacagagca gattgagtta ttatatattg gtattgcttc attagtggtt
      841 ttaattcttt cgacagtaat gagttttaga aaattatcaa aaaaatacac tcaaatttta
      901 aaaggagatg aacaatgaca attgaaattt tcaacgttac aaaaaaattc agctcaaaaa
      961 aaatatttac agatttaaat ttaacgtttg aagctggaaa aagctatgcc ttgattggag
     1021 gttctggctc tggtaaaagt actctgctta acattatagg aagactagaa aaaattgata
     1081 acgggaaagt tttagttgac gaacaagata tttggaaaat aaaagaaaga actttcttta
     1141 aaaatattat cgggtatgta ttccagaatt at
//
LOCUS       DAUCWU010000162         1150 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.165, whole genome shotgun sequence.
ACCESSION   DAUCWU010000162 DAUCWU010000000
VERSION     DAUCWU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.165"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..132
                     /locus_tag="VGY24_001764"
     CDS             <1..132
                     /locus_tag="VGY24_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ4284711.1"
                     /translation="IESLFYKEETIREVAAKLNISHPAVIKRRNKVLEKLKEMLEDF"
     gene            670..807
                     /locus_tag="VGY24_001765"
     CDS             670..807
                     /locus_tag="VGY24_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284712.1"
                     /translation="MKAIKVYSMRVAMLCRLYDLKLINDKEYTKIKNRIENDYKKVDR
                     K"
     gene            863..>1150
                     /locus_tag="VGY24_001766"
     CDS             863..>1150
                     /locus_tag="VGY24_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ4284713.1"
                     /translation="MNTKVKVIKASNTGARNRNSIHLDLKRVAAYCRVSTDSKDQLES
                     YKSQVDYYTNLIKNNKSWTLAGIYADEATTGTTATKRADFIRLISDCQNGDI"
BASE COUNT          464 a          140 c          215 g          331 t
ORIGIN      
        1 atagaaagtc ttttttataa agaagaaaca attagagaag tagctgctaa actgaatatt
       61 tctcatccag ccgttattaa aaggcgtaat aaagtcttag aaaaactaaa agaaatgcta
      121 gaagactttt agatgacagt taccaaatgg gtctaatttt cactgttact catacagtgg
      181 gaattggacc tttttattta tccaaattta tttttgaaaa ttggttacca agaaagactt
      241 cttttcactg ttaagtataa gagggtaatt ttaaacaatt ttgtttcatc aagataagta
      301 ttttctgatg gtcaaatttt aaaaagatta atagctctcg ataaattttg aaccttaaca
      361 attgaataga ccaagacaag acgttaatca tttttggagc gtgataacct atccgccaag
      421 atctttctgc tgaataattc ggcaaaacaa gtactgctaa gctaaaagca agggaagaag
      481 aaagggagcg ataagtaaga gtttgaatat agggagggat accctattcg ctatgaagaa
      541 aatgaggata atgatacttc taaggttgaa gccggctcat cctgaacaga ggcggaggtg
      601 agattcctat gttgctaatc caaggcactt gtcaatgtca aaaaacttaa atttacatag
      661 gaggttatta tgaaagcaat aaaagtttat agcatgagag ttgctatgtt atgtagactt
      721 tatgatctta aattaattaa tgataaggaa tatacaaaaa taaaaaatag aatagaaaat
      781 gattataaga aggtagatag aaagtagtta atcttgtgat ataataacac tgtagaaaga
      841 tacaaaatgg gaaggaggta aaatgaatac taaagtaaaa gtaataaaag cttcaaatac
      901 tggagcaaga aatagaaatt caatacattt agatttaaaa cgtgttgcag catattgtag
      961 agtaagtaca gatagcaaag accaacttga atcatataaa tcgcaagttg attattatac
     1021 aaatctaata aaaaataata agagttggac tttggctggt atatacgcag atgaagcaac
     1081 cacaggaaca actgcaacta aaagagctga ttttataaga ctaataagtg attgtcaaaa
     1141 tggagatata
//
LOCUS       DAUCWU010000163         1117 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.166, whole genome shotgun sequence.
ACCESSION   DAUCWU010000163 DAUCWU010000000
VERSION     DAUCWU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.166"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            34..1116
                     /locus_tag="VGY24_001767"
     CDS             34..1116
                     /locus_tag="VGY24_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEQ4284714.1"
                     /translation="MSKEIDKNKDENQEKKELQIRSSAAEYLTFIAANGDDQEAIEIR
                     YEDENIWLTQKMMAALYDVSVATINEHLKKIYADSELQEEATIRNFLIVQKEGSRNVS
                     RETKHYNLQAIIAVGFKVNNERAVQFRKWANQIVKDFTIKGWAMDDERLKNSGTKLTK
                     DYFEKLLVKIREIRLSERRFYQKITDIYATSLDYDPSAKATKRFFAAIQNKLHYAIHG
                     KTAAELIVDRANHKTKNMGLTTWEGSPDSKIHKYDVVVAKNYLNEEELNQMQRIVSSY
                     LDMAELQAERHIPMTMEDWEKRLNGFLQLWDKEILNDAGKVSAALAKKHAESEFEKYR
                     IIQDRLYKSDFDKFLELENDTKKLEE"
BASE COUNT          478 a          146 c          197 g          296 t
ORIGIN      
        1 aaggtagaat gaaaattata catggaggaa attatgagta aagaaattga taaaaacaaa
       61 gacgaaaatc aagaaaaaaa agaacttcaa atcagatcat ctgctgctga atacttgact
      121 tttattgcag ctaacggtga tgatcaagaa gctatagaaa ttagatatga agatgaaaat
      181 atttggctaa ctcaaaagat gatggcagct ctttatgatg ttagtgtcgc cacaataaat
      241 gaacatctta aaaaaatcta tgccgatagt gaattacaag aagaagcaac tattaggaat
      301 ttcctaatag ttcaaaaaga aggttctaga aatgtttcaa gggaaacaaa acactataac
      361 ctacaagcaa ttattgcagt aggctttaaa gtaaataacg aacgtgccgt ccaatttaga
      421 aagtgggcaa accaaattgt aaaagatttt acaattaaag gttgggcaat ggatgatgaa
      481 agattaaaaa attctggcac aaaacttacc aaggattatt tcgaaaaatt acttgtaaaa
      541 attcgtgaaa ttagattatc tgaaagaaga ttctatcaaa aaatcacaga tatttatgcc
      601 acatctttag actatgaccc atctgcaaaa gcaacaaaaa ggttttttgc agctattcaa
      661 aataaattgc actatgcaat tcatggaaaa actgcagctg aattaattgt agatagagcc
      721 aaccacaaaa ctaaaaatat gggacttact acttgggaag gatctccaga ttcaaaaatt
      781 cataaatatg atgtagttgt tgcaaaaaat tatctaaatg aagaagaatt aaatcaaatg
      841 caaagaattg tttcatcata tttggacatg gcagaactac aagcagaaag gcatatccct
      901 atgactatgg aagattggga aaaaagatta aatggatttt tacaattatg ggataaagaa
      961 atcttaaacg atgcaggaaa agtgtctgca gctcttgcta aaaagcacgc agagagtgag
     1021 tttgaaaaat atagaattat acaagataga ctttacaaaa gtgattttga taagttctta
     1081 gaactagaaa atgatactaa aaaattagaa gaataac
//
LOCUS       DAUCWU010000164         1070 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.167, whole genome shotgun sequence.
ACCESSION   DAUCWU010000164 DAUCWU010000000
VERSION     DAUCWU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.167"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>1070)
                     /locus_tag="VGY24_001768"
     CDS             complement(<1..>1070)
                     /locus_tag="VGY24_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ4284715.1"
                     /translation="DFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTK
                     GTTSNVQKVSFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTV
                     NESEEDRNISFSKPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTN
                     DFLISDLRPPICNINSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQI
                     FDKMESIYKYDNGGNYLKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRG
                     GRKNSLEEYQLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQM
                     FEKKYEEALLNGGHIEFYSKDL"
BASE COUNT          324 a          195 c          122 g          429 t
ORIGIN      
        1 tctaaatctt ttgaataaaa ttcgatatgc cctccattta atagagcttc ctcatatttc
       61 ttttcaaaca tttgcattat tggatcgctt aactctattg cttcaccttc tcctcctttg
      121 taatattcag gaaaacctat gcctgcatct ttatttatta cttcaacaaa gggaattgag
      181 cagttttctc catatttgcc aataaatttc aactgatact cttcaagtga atttttcctc
      241 ccccctcttc tctttacatt tgtatttaat tttacaaaca tctctgcgag tcgttctata
      301 ctttcccgag ccgtttcaga tagaatatta aaatcgcctt catactctgc taatgaatca
      361 acttttaagt aatttccacc gttatcatat ttataaatac tttccatttt atcaaaaatc
      421 tgcaaaagta agtctctacc ttccccaata tttaaatcgt tgtaatcttc aatcatcttc
      481 tttaaagtag ttaagtcagt acatagtgtt ccttcttcta atttagatag tagataatct
      541 aaggaattga tattacaaat tggtggtctt aaatctgaaa ttaaaaaatc atttgtaact
      601 aagtcgtgta tatatgactc tagcatgtct gacactatat ctggataatt atttttaaat
      661 acatctatta actcttcata ttttatatat cgagttttac atttttctac aagtaactta
      721 attggttttg aaaaacttat gtttctatct tcctctgatt cattcactgt aaatggtagt
      781 acatatctgt caccttgagt aaacgccgct gtatttattt ttaatgaaag tttttcaagg
      841 ttacttattt catacgattt tactagagaa tatatccatg aataatctgg gactgctttt
      901 ttaataaaac taaccttttg tacattagaa gtggttccct ttgtcaaatc aactgtatta
      961 atagcagtaa agataccaaa tggagtagtt ctaaacgctg ctcttttaga ataactattt
     1021 aatgagactt ttacattttg ctttttcttt tcattacatt tatcaaaatc
//
LOCUS       DAUCWU010000165          981 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.168, whole genome shotgun sequence.
ACCESSION   DAUCWU010000165 DAUCWU010000000
VERSION     DAUCWU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.168"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..167
                     /locus_tag="VGY24_001769"
     CDS             <1..167
                     /locus_tag="VGY24_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ4284716.1"
                     /translation="INGGGKDGANIFLTGMAGAAEGVTVCAQTGVFIPWQGYILCGAA
                     GAATNIIWPH"
     gene            217..497
                     /locus_tag="VGY24_001770"
                     /pseudo
     CDS             217..497
                     /locus_tag="VGY24_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            691..>981
                     /locus_tag="VGY24_001771"
     CDS             691..>981
                     /locus_tag="VGY24_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284717.1"
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDL
                     FVFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVS"
BASE COUNT          312 a          125 c          189 g          355 t
ORIGIN      
        1 tcataaatgg aggcggaaaa gacggagcaa atatcttttt gacagggatg gcaggtgctg
       61 cggaaggagt gacagtttgt gctcagacag gcgtatttat cccttggcaa ggttatattt
      121 tgtgtggagc agctggtgct gctacaaata ttatctggcc tcactaaata tataaatgac
      181 tatctatcac tgatgtataa gtagtagagg agtggtatga aaattttaga tattattttt
      241 aacctatctg atagaaagat gatatttcta gaaaatgttg aaaagctggg aggttacatt
      301 gtccctttta cccttatcat cttgagtgta gcctacaact ttaggcaccc ttatcctatt
      361 tgggctgctg tactaggagg tatatccctt attgtgatct ttatttattt tatacttatt
      421 gtttatctat atttgcgtta tagatatttg gtatggaaaa aataccagaa attaagatta
      481 attttactga gctataatgc aatcacattt aaggggaaaa tggatgactt atacaatatc
      541 agtacttatg atatttaact ttagagacta aaaagttaaa agcaaaatgc gtattgaaaa
      601 ttggttaaaa aagttataac tgtctctgat aacaggaata acaatttgtt cggttatatt
      661 tgctaccgat agagtagagg aggtgaagat atgcgtaata tattaagatt tttaggtatt
      721 gttattattt ctgcagtgat tctatttagt ataggtagtt tctacgattt accattaatg
      781 aaaaatatac aacttatctg ttggagtttt atccttgatt tattcgtttt tgtctacaaa
      841 caaagacaaa cgacagaagt gctcacatgg tatcaagttg ttaagcaatt ttggttattt
      901 attaagtgta ctattcttat tcctacctta attgctttta ttataataat aggatgtttg
      961 acgagtatat cagaggtatc a
//
LOCUS       DAUCWU010000166          974 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.169, whole genome shotgun sequence.
ACCESSION   DAUCWU010000166 DAUCWU010000000
VERSION     DAUCWU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.169"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            172..>974
                     /locus_tag="VGY24_001772"
     CDS             172..>974
                     /locus_tag="VGY24_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ4284718.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQIPSNPNLYQETCAYKDSDLTLPVGRLGVNQPLPIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYG"
BASE COUNT          374 a          155 c          144 g          301 t
ORIGIN      
        1 aatgatttat cctattccag gactattacc aaaatgatga tggacaatat tgtaaaaaaa
       61 atagaagcat ttcttgcttt ttctgataaa aaattagcag agctgcaaca ggaaaatcaa
      121 aaagttaaag aagaaagtca gtatattcaa aaaaactaga aaggagtcaa tatgagaaaa
      181 ttattagcgg ctatgttaat gacttttttt ctgactcctt taccagtgat tagtacagaa
      241 aaaaaactta tattttcaaa aaatgctgtt tatcaattga aacaagatgt cgttcaatca
      301 acacaattct ataatcaaat accctctaat ccaaatcttt atcaagaaac gtgtgcctat
      361 aaagacagtg atttaactct accagtagga agattaggtg taaatcaacc attacctatt
      421 aaatcgcttg tgcttaacaa agaatcttta ccggtttttg agttagctga tggtacctat
      481 gttgaggcta atcgacaatt gatttatgac gatattgtac ttaatcaagt agatatagat
      541 agctattttt ggacacaaaa gaagcttagg ctttattcag ccccttatgt tttaggtacg
      601 caaacaattc cttcttcttt ttcatttgct caaaaagttc atgccactca aatggcacaa
      661 acaaaccatg gaacttatta tcttattgat gataagggct gggcatcaca agaagatcta
      721 gttcaatttg ataaccgcat gttaaaagtc caggaaatgc tcttacaaaa atataataac
      781 ccaaattatt caatttttgt aaaacaactc aacacacaaa caagtgctgg tattaatgct
      841 gataaaaaaa tgtatgctgc aagtatctcg aaattagcac cactttatat tgttcaaaaa
      901 caattacaaa aaaagaaatt agcagagaat aaaactttga cttatactaa agatgttaat
      961 catttttatg gaga
//
LOCUS       DAUCWU010000167          970 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.170, whole genome shotgun sequence.
ACCESSION   DAUCWU010000167 DAUCWU010000000
VERSION     DAUCWU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.170"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>970
                     /locus_tag="VGY24_001773"
     CDS             <1..>970
                     /locus_tag="VGY24_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ4284719.1"
                     /translation="ARNTLDTLKYVRLLKENNVGVVFEEENIDTLTMDGELLLTILSS
                     VAQQEVENTSAHVKKGLKMKMEKGELIGFQGCLGYDYDPTTKSISINEEEAKIVRYIF
                     KRYLEGNGGSVIGRELEEQGYLTPRGKTKWSDTTVLGIIKNEKYIGDILMGKTFTVDP
                     ITKRRLANFGESDKYHIENHHEPIISREDFEKAQEIRLRRAQNRNTIANKDRKREKLS
                     RQYAFSSMLECGFCGEILSRRTWHTSSIYKKINWQCVKSTKKGKKYCPHSKGIQEAAI
                     EKAFVESYRQLCHADSTVIDDFLKIVEEEINDNTLVKDLKKLENQLN"
BASE COUNT          414 a          132 c          190 g          234 t
ORIGIN      
        1 tgcaagaaac acattagata ccttaaagta tgtaagactt ctaaaagaaa acaatgttgg
       61 cgtagtattt gaagaagaaa acatagatac tttgactatg gatggagagc tattactaac
      121 aatattaagt tccgtagctc aacaagaagt agaaaatacc tcagcccatg taaaaaaagg
      181 actgaaaatg aaaatggaaa aaggggagct aattggtttt caaggttgtc ttggatacga
      241 ctatgaccct acaacaaaaa gtatctctat aaatgaagaa gaagccaaaa tagtcagata
      301 catctttaaa agatatttag agggcaatgg tggctcggtc attggcaggg aactagaaga
      361 gcaaggatat ctcaccccga gaggtaaaac aaaatggtct gatactacag tgttaggaat
      421 aattaaaaat gaaaagtata ttggagatat actaatggga aagaccttca cagttgatcc
      481 cataacaaaa agaagactag caaactttgg tgaatctgat aaatatcata ttgaaaatca
      541 tcacgagcca attatatcaa gagaagactt tgaaaaggca caggagatta gactcaggag
      601 agcacagaat agaaacacca ttgctaacaa ggataggaag agagaaaaac tatcaagaca
      661 atacgctttt tcaagtatgc tggaatgtgg attttgtggt gaaatacttt caagaagaac
      721 atggcacact agttcaattt ataaaaaaat taactggcag tgtgtaaagt caacaaaaaa
      781 aggtaaaaaa tattgccctc attcaaaagg aatacaagaa gcagcaatag aaaaagcatt
      841 tgttgaaagc tataggcaat tatgccatgc agattcaaca gtaatagatg attttttaaa
      901 aattgtagaa gaagaaataa atgataatac tttagtcaaa gatttgaaaa agttagaaaa
      961 ccaattaaac
//
LOCUS       DAUCWU010000168          936 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.171, whole genome shotgun sequence.
ACCESSION   DAUCWU010000168 DAUCWU010000000
VERSION     DAUCWU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.171"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>936
                     /locus_tag="VGY24_001774"
     CDS             <1..>936
                     /locus_tag="VGY24_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEQ4284720.1"
                     /translation="TDEKVLVSSFKCHPSTAHIQFLKTREDNDTKGTVLARHLIQSFL
                     PGEVDPIKAHEIGMELCKKILKEDYEFVLATHIDRGHIHNHIIFNNVNYKTGKCYQSN
                     KKSYHKIRYQSDELCKENKLSVIDKYYEAYKRKYKTAGKSWYEYDQNKKGNSWKSKLQ
                     FDIDRMINKSNSWEEFLDNMKSIDYEIKFGKHIAFRHKDKQRFTRAKTIGEDYTEEKI
                     KERIDLAIKNKANPIKKRVGNVIDISTNEKAQSSKGYEVWARKHNIKTMADSIIKLRE
                     QGIKSITKLDDLIKKSADDRQDLLDKIKKIESEMK"
BASE COUNT          400 a          120 c          149 g          267 t
ORIGIN      
        1 aaactgatga aaaagtctta gtatcttcat tcaaatgtca tccatctact gcccatattc
       61 aatttttgaa aactcgagaa gataatgata ctaaaggaac agtcttagct agacatttaa
      121 ttcaatcttt tctaccagga gaggttgatc ctataaaagc tcacgaaatt ggaatggaat
      181 tatgtaagaa aattttaaaa gaagattatg aatttgttct tgcaactcat atagatagag
      241 gacatatcca taaccatata atttttaata atgttaatta caagactggt aaatgctacc
      301 aatctaacaa aaaatcttac cacaaaatca ggtatcaaag tgatgaatta tgtaaagaaa
      361 ataagctttc agtcattgat aaatactatg aagcttacaa gagaaaatat aaaactgctg
      421 gtaagtcttg gtacgaatat gatcaaaaca agaaaggaaa ttcttggaag tctaaacttc
      481 aatttgatat agatagaatg atcaataagt ctaactcgtg ggaagaattt ttagataata
      541 tgaaatctat tgattatgaa attaagtttg gtaaacacat tgcttttcgt cataaagata
      601 agcaaagatt tacaagagct aagactatcg gagaagatta tactgaggaa aaaatcaaag
      661 aacgaataga tttagcaatt aaaaataaag ctaatcctat taaaaagcgt gtaggaaatg
      721 ttattgatat atccactaat gaaaaagctc aatcctcaaa aggttatgaa gtttgggcaa
      781 gaaaacataa tatcaaaaca atggctgact cgataattaa gcttagagaa caaggaatta
      841 aatctattac taaactcgat gatctaatca aaaaatctgc tgatgataga caagacttgt
      901 tagataaaat aaagaaaatc gaaagtgaaa tgaaaa
//
LOCUS       DAUCWU010000169          927 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.172, whole genome shotgun sequence.
ACCESSION   DAUCWU010000169 DAUCWU010000000
VERSION     DAUCWU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.172"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..844
                     /locus_tag="VGY24_001775"
     CDS             <1..844
                     /locus_tag="VGY24_001775"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ4284721.1"
                     /translation="LDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKANDTA
                     TMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVVRNDLEYSIRIN
                     FPLISKFFNNDIESQLYVDKHVEKIKKYIEQKYKNISFSIDYHYYLTTTGSCIDFGEE
                     GAVGRGNKTHGIISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTPCQI
                     IMQLNIGSKLYRPTHLFIQTEESVDQERVLEIVNRHLNNGKQNTNLILSTQHFIPKTN
                     VYDG"
     gene            828..>927
                     /locus_tag="VGY24_001776"
     CDS             828..>927
                     /locus_tag="VGY24_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ4284722.1"
                     /translation="MFMMDKTVLYFGLVEISFDVPDFIFQKIKEQNN"
BASE COUNT          352 a          108 c          155 g          312 t
ORIGIN      
        1 tttagatgtt gaaaattatg tagaatttga gacattgacc tcagatttta ctacaaaaac
       61 taattggttc tcgcctgaag caatagaaga tttaccggag tatctagatg taccaaaggc
      121 aaatgataca gctacaatga taagttattg gccattaaca atttctgaag aattagcatt
      181 aatgattgaa gggtattttt ataaattaga taagaatgaa ttaccaacgc caagatttac
      241 taaaatgggt ggtgatatta aagtaatggt cgtaaggaac gatttagaat attctattag
      301 aataaatttt cctttaataa gtaaattttt taacaatgat attgaaagtc agctgtatgt
      361 tgataaacac gtagaaaaaa ttaagaaata tattgaacaa aagtataaaa acataagctt
      421 ttctattgat taccattatt atttaactac tacaggttct tgtattgatt ttggagaaga
      481 aggtgcagtt ggtcgaggta ataaaactca tggtatcata tcaagtttta ggccaaatac
      541 tatggaagca ccagctggaa aaaattgtac ttattttgta ggaaaggttt ggggattttt
      601 gtctgatact attgccaaag aaatttatga agcattcaat actccctgtc aaatcattat
      661 gcaattaaat attggatcaa aattgtatag accaacccat ttatttattc agacagaaga
      721 aagtgttgat caagaaagag tattagaaat tgttaacagg catttaaata atggtaaaca
      781 aaatactaat ttaatcttgt caactcaaca ttttattcct aaaaccaatg tttatgatgg
      841 ataaaactgt tttatatttt ggattggttg aaatttcttt tgatgtgcct gactttatat
      901 ttcaaaaaat aaaagagcag aataata
//
LOCUS       DAUCWU010000170          903 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.173, whole genome shotgun sequence.
ACCESSION   DAUCWU010000170 DAUCWU010000000
VERSION     DAUCWU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.173"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>903
                     /locus_tag="VGY24_001777"
     CDS             <1..>903
                     /locus_tag="VGY24_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ4284723.1"
                     /translation="KNINATSSEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNI
                     LKINKKATIVIKSTVPIGFTEYLRNQFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGN
                     ESRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEM
                     QNLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAPIITSISE
                     SNLLRKIHIAKTILNSSAKTIGIYRINSKKDSDNCRESSTIDVAKLLKSSGKDVIIFE
                     PLINQKKFLGCPLINDFNEFIKYSD"
BASE COUNT          327 a          131 c          144 g          301 t
ORIGIN      
        1 aaaaatatta atgcaacttc ttctgaagaa ttagcatata aggatgcgac tttcataatc
       61 ttgtctttgc caaccaacct aaaatttaat aagcttgata cttccattat cgaaatttct
      121 gtaagtaata ttttaaagat aaacaaaaag gctacaattg taataaagtc gacagttcca
      181 attggtttta cagaatattt aaggaatcaa tttcactaca acgatatcat tttttcacct
      241 gagttcctta gggaaggatc aactattcat gatcaattgt atccttcgag aactatagtt
      301 ggaaatgaat ctagaaattc tcaattattc ttagacatac taacagatat atcggttgaa
      361 aaagactcgc catctttatt agttggctct tctgaagcag aagcgataaa gttattttcg
      421 aatgcatact tggcacaaaa aattgctttt tttaatgagt tggatacgtt tgctgaaatg
      481 caaaatttgg actcaaaaaa aattattgag gctatgggat atgaccagag aataggaaat
      541 tcgcacaata atccttcttt cggttttggt gggtactgtc ttcctaagga tattaagcaa
      601 ttagagtatc attttaaaga aattccagca ccaattatta ccagtataag tgagtctaat
      661 ttattaagaa aaattcatat agcaaaaacg attttgaaca gctcagctaa aacaatagga
      721 atttatagaa ttaattccaa aaaagattcg gataattgta gggaatcttc tacaattgat
      781 gttgctaaac ttctaaaaag cagtggtaag gatgttatca tctttgagcc cttaattaac
      841 caaaaaaagt ttttggggtg ccctttaatt aatgatttta atgaatttat taaatattcg
      901 gat
//
LOCUS       DAUCWU010000171          892 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.175, whole genome shotgun sequence.
ACCESSION   DAUCWU010000171 DAUCWU010000000
VERSION     DAUCWU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.175"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            61..594
                     /locus_tag="VGY24_001778"
     CDS             61..594
                     /locus_tag="VGY24_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ4284724.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            complement(748..>892)
                     /locus_tag="VGY24_001779"
     CDS             complement(748..>892)
                     /locus_tag="VGY24_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ4284725.1"
                     /translation="EATLGRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYN
                     KML"
BASE COUNT          328 a          148 c          149 g          267 t
ORIGIN      
        1 agagaaaaaa aatcactttt ggacattatg tctaaaagtg ttcaaaagga gagatggtag
       61 atggtcaacc gtaaagaaaa tacgaagcaa gcaattttaa aagcaatggt tatgttatta
      121 aaaaccgaaa gttttgacga tattaccaca gttaaattat ctaaaagagc tggaattagt
      181 cgctctagtt tttacacaca ctataaagat aaatatgaaa tgattgacta ttatcagcaa
      241 accttttttc acaaattaga atatattttt gagaaaaaat atcaaaataa agaacaagcc
      301 ttccttgaag tatttgaatt tctccaaaga gaacagcttc tctcttccct tttgtctgct
      361 aacggtacca aagaaataca ggcctttatc atcaataaag ttcgcctact catcactact
      421 gatttacagg acaagttccg caccgaagag ttatctcaaa cggaaaaaga ataccaaagt
      481 atctacttag cccatgcttt ttttggtgtc tgtcaaagtt ggattgctaa aggtaaaaaa
      541 gaatcacctc aagaaatgac acaattcgtt ctcaaaatgc ttacgagtac ctaaatagta
      601 acccttacga aagtatcatt ttacaagtca tcagccttag tgataacttt tttagaagcc
      661 atagtaaaat gaagggatca gaatagattt gttatgagat acaaaaaaag cctgttaaat
      721 aggctttttg taagtttaat cttaaaatta aagcattttg ttatagaatt caacgacaag
      781 tgcttcattg atttctgggt taatttcatc gcgttctggg aggcgagtca atgaaccttc
      841 aagtttttca gcatcaaatg atacgaaagc tggacgtcca agagtagctt ca
//
LOCUS       DAUCWU010000172          837 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.176, whole genome shotgun sequence.
ACCESSION   DAUCWU010000172 DAUCWU010000000
VERSION     DAUCWU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.176"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..72
                     /locus_tag="VGY24_001780"
     CDS             <1..72
                     /locus_tag="VGY24_001780"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEQ4284726.1"
                     /translation="GGKGMGMFSIFVIERFHYQPNQK"
     gene            130..>837
                     /gene="erm(A)"
                     /locus_tag="VGY24_001781"
     CDS             130..>837
                     /gene="erm(A)"
                     /locus_tag="VGY24_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEQ4284727.1"
                     /translation="MKQKNPKNTQNFITSKKHVKKILKYTNINKQDKIIEIGSGKGHF
                     TKELVEMSQRVNAIEIDEGLCHATKKAVEPFQNIKVIHEDILKFSFPKNTDYKIFGNI
                     PYNISTDIVKKIAFDSQAKYSYLIVERGFAKRLQNTQRALGLLLMVEMDIKILKKVPR
                     AYFHPKPNVDSVLIVLERHKPFILKKDYKKYRFFVYKWVNREYHVLFTKNQLRQVLKH
                     ANVTDLDKLSNEQFLSVF"
BASE COUNT          322 a          101 c          152 g          262 t
ORIGIN      
        1 ggaggtaaag gtatgggtat gttttctata tttgttattg agagatttca ttatcaacca
       61 aaccaaaaat aattggttat aatgagctct taagattagt taattataac cggcaaggag
      121 aaggttatta tgaaacagaa aaacccgaaa aatacgcaaa atttcattac atctaaaaag
      181 catgtaaaga aaatattaaa atatacgaat atcaataaac aagataaaat aatagaaatt
      241 gggtcaggaa aaggacattt taccaaggaa cttgtggaaa tgagtcaacg ggtgaatgct
      301 atagagattg atgaaggttt atgtcatgcc acgaaaaaag cagttgaacc ttttcagaat
      361 ataaaagtta ttcatgagga tattttgaag tttagctttc ctaaaaatac agactataaa
      421 atatttggta atattcccta caatattagt actgatattg taaaaaagat tgcttttgat
      481 agtcaagcga aatatagcta ccttattgta gagaggggat ttgctaaaag gttgcaaaat
      541 acccaacgag ctttaggttt gctgttaatg gtggaaatgg atataaaaat tcttaaaaaa
      601 gtgccacgag catattttca ccctaagcct aatgtagatt ctgtattgat tgtacttgaa
      661 aggcataaac catttatttt aaagaaggac tacaaaaagt atagattttt cgtttataaa
      721 tgggtaaaca gggaatatca tgttcttttt actaaaaatc aattaagaca ggtgctgaag
      781 catgcgaatg ttactgatct tgataaatta tccaatgaac aatttttgtc tgttttc
//
LOCUS       DAUCWU010000173          812 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.177, whole genome shotgun sequence.
ACCESSION   DAUCWU010000173 DAUCWU010000000
VERSION     DAUCWU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.177"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..812)
                     /locus_tag="VGY24_001782"
                     /pseudo
     CDS             complement(1..812)
                     /locus_tag="VGY24_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          216 a          181 c          157 g          258 t
ORIGIN      
        1 gaatgccaaa gaaggaatcc atcatgccgt tatccgggct attccctttg cgggacatgg
       61 atggcagaat acctttagac tctaaaaaat catggtagga ttggtgttgg tattgccacc
      121 cctggtcact atgaagaata gttccgctgt atgaatccgc tggaaaagtc ttctcaagca
      181 tggtttgaac ctgtttcaag ttaggagacc gagatagagt gaaatcaata atctcactgt
      241 tatagccatc aagaacaggt gataagtaca gttttccttc aggtaaggcc aactccgtca
      301 catcagtgta acatttttcg taaggtttag agccttcgaa gtgacgttta atcagattat
      361 cagccttctt accaacctct cctttgtaag aagaatactt gcgcttccga cgaatgcggg
      421 ctgctaggcc catgactttc atgagacgtt gaactttctt atgattgacc acaaatcctc
      481 ggttacgcaa ctccatatga atccgacgat aaccgtaatt acctttatgc tcatcataaa
      541 tctctcgaat cacatgcttt agttttatat ccttatctcc ttgagctagt cgcttcactt
      601 gatagtaata ggtggagcgt gataaatcaa ggatctcaag tagcatctct aagggaaatt
      661 gattccttaa tgcttgaatg atttctgttg ctctttgagt tttgcttcgt ctctcaagcg
      721 gtattctctc agctttttta gcacagcatt ctccgctcta aggtattcta attccttctg
      781 aagacgttca acctctgtca tctcttccaa at
//
LOCUS       DAUCWU010000174          787 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.178, whole genome shotgun sequence.
ACCESSION   DAUCWU010000174 DAUCWU010000000
VERSION     DAUCWU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.178"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..271
                     /locus_tag="VGY24_001783"
     CDS             <1..271
                     /locus_tag="VGY24_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284728.1"
                     /translation="YKYHKKNPEDNQFAEEYYSELSVYKIAAKEILENYKKLPSTKEI
                     LSNLDKLQEKKNTLMQEYSLNKEQFSDLVQYRKNYENYYGKEVER"
BASE COUNT          304 a          116 c          106 g          261 t
ORIGIN      
        1 ctataaatac cacaagaaaa atcctgaaga taatcagttt gcagaagaat attatagtga
       61 actttctgtt tataaaatag ccgctaaaga aattttagaa aactataaaa agcttccaag
      121 cacaaaagaa atactatcaa atctcgataa attgcaagaa aaaaagaaca cccttatgca
      181 agagtattct ttgaataaag aacaattttc tgaccttgtt cagtatagga aaaactatga
      241 aaattattat ggaaaggaag tggagagata aaaagctata agcggataaa cttatagctt
      301 ttttaaaatt atttacaaat ttttattcta aacttattat cttacttaaa gagatcctac
      361 tagatgacta gcaaacattg ctataggaag taataagata aaaaggacat ttaatgccat
      421 aaaaaaccta tctttttccc ttaccccaag tattaaacct actagtccaa taattggaca
      481 gacaagcatg tgagaataag caataaatac gcatctcata atttattata tcatttttta
      541 taaaagaaac ctgctaacaa aagtcaagta taacctgata taaatttcag gttatttgat
      601 tatttgagtt attaaattag cgtatagcaa acaagccgag tgtaatcaat cggcttgctt
      661 tttagtgtta ttttaaatct tatgctactt ttaattcttt tttctctgct tttgattcca
      721 taactcttgc atagtagatt cttaagaatt tgtttaagcc tgcaaatttg caaacgtttt
      781 ggctttc
//
LOCUS       DAUCWU010000175          785 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.179, whole genome shotgun sequence.
ACCESSION   DAUCWU010000175 DAUCWU010000000
VERSION     DAUCWU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.179"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..419
                     /gene="rlmH"
                     /locus_tag="VGY24_001784"
     CDS             <1..419
                     /gene="rlmH"
                     /locus_tag="VGY24_001784"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ4284729.1"
                     /translation="EYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEAQRIHK
                     KIGERDFVIALAIEGKQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKRANM
                     LMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(575..648)
                     /locus_tag="VGY24_001785"
     tRNA            complement(575..648)
                     /locus_tag="VGY24_001785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(612..614),aa:Arg,seq:tct)
BASE COUNT          287 a          122 c          130 g          246 t
ORIGIN      
        1 tagaatatca aaaaagactt tcccgttttt gtcaattcga aatgattgag ttaactgatg
       61 aaagaacacc agataaagca agttttgctg ataatcaatt gattatgagt aaagaagctc
      121 agcgaatcca taaaaaaatt ggggaacgtg attttgtaat tgcattagct attgaaggaa
      181 aacagtttcc ttcagagaca tttagtgaat taatatcagg ggttactgtt aagggatatt
      241 caactatcac gtttattatt ggtgggagtc ttggattaga ttcaattatt aaaaaaagag
      301 ctaatatgct aatgagtttt ggattgttaa ccttacctca tcagttaatg cgtctagtcc
      361 ttactgagca aatttatcgt gcttttatga ttacacaagg cagtccttat cataagtaag
      421 agagtgtgaa ctggaataac taactcttac cactttatta gataatagtt tggtataatt
      481 aaaagcaaat attaaaaaaa gacagactaa atcctatctt ttcaatacac taaggtaaat
      541 acaaaaaagc actaaaacag tgctttttta tctttgatcc cagcaggatt cgaacctgcg
      601 accgttcgct tagaaggcga atgctctatc cagctgagct atgagacctt aaccacatca
      661 ttttaacaaa aattatctaa aactgtcaat atttatttta aaaaactaga aaataacatt
      721 atattccgcg ttgtaaaatg aagtgcaaca aaaaagacat gttcgtctga tatactagaa
      781 ttccc
//
LOCUS       DAUCWU010000176          760 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.180, whole genome shotgun sequence.
ACCESSION   DAUCWU010000176 DAUCWU010000000
VERSION     DAUCWU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.180"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>760
                     /locus_tag="VGY24_001786"
     CDS             <1..>760
                     /locus_tag="VGY24_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ4284730.1"
                     /translation="AGVIISKNSSMLKQTHAGYFMAGHKRIELGEKIKKVPMGFFSSL
                     SLGNLTTIATTNLDNVETWVPTLCIMVFGGMLNAIVFILSLFLFSYKVGLVAIVGSIV
                     FLMIIARMQKRSSKNADKIHEIQVSLTKEVLSTLQGMQIIKSYNLRGSNNKKLDKSFD
                     GASKYSFDLEKTIMPYSLLAKIIIAITICLMLFISIKLYFVENIQIVNTIMILIASFV
                     IFDGLITSGSAMAMLRMVENAIDSYSYVNDMEDM"
BASE COUNT          270 a           91 c          129 g          270 t
ORIGIN      
        1 tagcaggagt aattatttca aaaaattcct ctatgctcaa acaaacacat gccggatatt
       61 ttatggctgg acacaagaga attgaattag gtgaaaaaat aaaaaaagtt cccatgggat
      121 tcttctcaag tttaagttta ggaaatttaa caactattgc gactactaac ttagataatg
      181 tagagacttg ggttccaact ttgtgtatta tggttttcgg tggcatgctt aatgctattg
      241 tttttatctt atccttattt ttgttttctt ataaggtagg tcttgtagca atagttggtt
      301 ctattgtatt tttaatgatt attgctcgta tgcaaaaaag gtctagtaaa aatgcagaca
      361 agattcatga gattcaggta tcacttacca aagaagtctt atcaacattg caaggaatgc
      421 aaattataaa atcatacaat ctaagaggaa gtaacaataa aaaacttgat aaaagctttg
      481 atggtgcaag taaatattct tttgatttag aaaaaactat aatgccatat agtttattag
      541 ctaaaataat aatagctata acaatttgtt tgatgttgtt tatatcaata aaattgtatt
      601 ttgttgaaaa cattcaaata gtcaatacta ttatgatact tatagcaagt tttgtaatat
      661 ttgatgggct tataacatct ggttctgcta tggcaatgct aagaatggtt gagaatgcaa
      721 tagattctta ttcctatgta aatgatatgg aagatatgaa
//
LOCUS       DAUCWU010000177          726 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.181, whole genome shotgun sequence.
ACCESSION   DAUCWU010000177 DAUCWU010000000
VERSION     DAUCWU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.181"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            150..>726
                     /locus_tag="VGY24_001787"
     CDS             150..>726
                     /locus_tag="VGY24_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4284731.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLKTLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEI"
BASE COUNT          268 a          102 c          132 g          224 t
ORIGIN      
        1 ttaatacaat taactcaaaa aagccacaca ttcctagtac aattatcctg ctatattcta
       61 acaaatagtt aaagttcggt ttttatttgt aatatagata aaattgttaa aaaacaagcg
      121 tcttgtaaac catttcaaag tatgatagaa tgaaaaagta ccttaaaaag ataggaaaaa
      181 tgatgacaaa gatgtattat gatgaaaatc cagatagcct acacgatatt catgaagtaa
      241 aagtagagct gcttaatcat ccctttactt ttttgacgga ttcaggtgtc ttttcaaaga
      301 aaatggttga tttcggtagc caagtattat taaagacact aaatttcaag gagaacgaac
      361 gtgtactgga cttaggttgc ggctatggtc ctcttgggat tagtctagcc aaggttcaaa
      421 gggtagacgc aaccttagtg gacatcaata atcgtgctct tgatttagct agaaaaaatg
      481 caactaataa ccaagtggca gtaactattt ttcaatcaaa tatttatgaa aatattagtg
      541 gccattttga gcatattatt agtaatcctc ctattagagc aggaaagaga gtagttcata
      601 gtattattga aaaaagcata gattttttag tggttaatgg tgacttaacg attgtgattc
      661 aaaaaaaaca aggagctcct agcgctaaag caaaaatggc aactattttt ggtaatgttg
      721 agatat
//
LOCUS       DAUCWU010000178          689 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.182, whole genome shotgun sequence.
ACCESSION   DAUCWU010000178 DAUCWU010000000
VERSION     DAUCWU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.182"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..239)
                     /locus_tag="VGY24_001788"
     CDS             complement(<1..239)
                     /locus_tag="VGY24_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEQ4284732.1"
                     /translation="MANRIRNERIEIKLTKEEKEIFEKKMKLANCKTMSHFLRKCVLE
                     KEIYVVDLEPFRNLQWLLSNATNNINQIAKATNTTG"
     gene            534..>689
                     /locus_tag="VGY24_001789"
     CDS             534..>689
                     /locus_tag="VGY24_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ4284733.1"
                     /translation="MKMEADERKKQIRQAAIKVFLDKGFRNTVMNDIMEATGLSRGGL
                     YHHYGSTH"
BASE COUNT          219 a           93 c          117 g          260 t
ORIGIN      
        1 ccagttgtat tagtagcttt tgcaatctga tttatattat ttgttgcatt tgaaagtaac
       61 cattgtaggt ttctaaatgg ttctaaatct actacataaa tttctttctc taatacacat
      121 tttctaagaa agtgggacat agttttgcaa ttagcaagtt tcattttctt ttcaaaaatt
      181 tctttttctt ccttagttaa ttttatttct attctttcat ttctaattct atttgccatt
      241 ttagtctcct ttataaaata tatttcgggg tcttagggtt ctccctaaca aggtaaaaat
      301 taaaaaaatg gagcattccg taaaggtctg ctccattaat ttttttcgta agtgtccgta
      361 cacttactgt gcttgctatt aaagttataa gcgttaagcg tgtattaagt tatgattaat
      421 tataccgcat ttaaaaaaat taataaagaa gtataagcga agctattgac aatgattatc
      481 agagttgata caataatacc gacaagagtg tcggtttgga ggttatttat gaaatgaaaa
      541 tggaagctga tgaaagaaaa aagcagatta gacaagcagc tattaaagtc tttttggata
      601 agggatttag aaatacagtt atgaatgaca ttatggaagc tactgggctt tctagaggtg
      661 gcttgtatca tcattatggt tctacgcat
//
LOCUS       DAUCWU010000179          599 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.183, whole genome shotgun sequence.
ACCESSION   DAUCWU010000179 DAUCWU010000000
VERSION     DAUCWU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.183"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          200 a          115 c           76 g          208 t
ORIGIN      
        1 tcacttttaa tttctctatt caataaagtt ctgtgctaat ctacctcacc ctatggattt
       61 ttaatcccga aacatcaaat gtcatctgtc aaatgcccta tttaaaaggg tcaaaaaacg
      121 gtatttttta gcctgttttt gacccaattt tcgtactcaa accactacat gttgtggttg
      181 actcctatta ttccttgtcc aaaccacaat acgtcatcag aattgctgct tcaacgtggg
      241 acgagttgat agaattgatg tatttatgat ttttagcgaa actgttgtat atgggaacat
      301 atcgactaat ttgtacgttc gtgggaacat aacgactagc tttctaatgg tcgccaaaag
      361 aatcgaaata taattctagt ttagaatact tctatcctta tcaaatatat gaaactgtaa
      421 taataaaatt taattgcttt catcgactaa tgttaaaacc tacaacacaa gaatttaaat
      481 cattagatta cactatttac tattcaatca atttaagctc cttcattcat atcccttcta
      541 ctatacccca agaaacctat tactattaaa gcaatgctaa ttatagtaac tgtaagaaa
//
LOCUS       DAUCWU010000180          598 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.184, whole genome shotgun sequence.
ACCESSION   DAUCWU010000180 DAUCWU010000000
VERSION     DAUCWU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.184"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(280..555)
                     /locus_tag="VGY24_001790"
     CDS             complement(280..555)
                     /locus_tag="VGY24_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ4284734.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT          200 a          118 c           89 g          191 t
ORIGIN      
        1 ataaataagt caagtaattt agaaacattc taaataaact tttttaaact taccttttgc
       61 tttcaaaaat cttaacttca actatttgca aatatgaaaa aaactccccc tacctgcgga
      121 aatgatatac taatagaagg atgactgacc attatagagt gcatgcttta gaacttggct
      181 taactgaaga gaaaactact aactttatgc cccaaatata aaaaaagaac cttgtcgcta
      241 tcaataaaca tcgatagcaa caagattttt ctagacgaat tatttaacag cgtctttaag
      301 agctttacca gctttgaagg ctggaacttt tgaagctgca atttcaattt ctgcaccagt
      361 ttgtgggtta cgacctttac gagctgcacg ttcgcgtact tcgaagttac caaaaccgat
      421 caattgtact ttttcacctt cagcaaggaa agcttcgatt gtagaaaaca cggcatcaac
      481 tgctgctgct gaatcttttt tagtcaattc agttgcttct gcaacctttg cgattaaatc
      541 ttgtttgtta gccatttaac aaatcctcca aataattagt aagcttaatg ctttaaca
//
LOCUS       DAUCWU010000181          582 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.185, whole genome shotgun sequence.
ACCESSION   DAUCWU010000181 DAUCWU010000000
VERSION     DAUCWU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.185"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          205 a           53 c          105 g          219 t
ORIGIN      
        1 atttatagat atagtagtga ggaacattcg ttatatttat taccacttta tttattgaga
       61 gtatacaatt atctaaagta caactaagtt ttcaattttt attaatcttg agaagaagtg
      121 tgattggtgg aatatatttc aatatgttta gcttatctaa ataataaata cagtatgtaa
      181 gtaaatctat ttataatatt tttgatactt tgagtagtaa cgataatatt taattttttg
      241 atggattatt ttaattgact ttagtgggtt agatgccagt ttggattttt gatatttgtg
      301 ttgagtttaa aagataaaaa cacttaatga aaatctgttt tttcttagaa aggggaccag
      361 tatgattgag acgctaatga gtacgttgga aatggttaga aaaaaaattc tagtaggact
      421 gcaatcgaag atgactttga ggaatataaa aagagaaatc taccaatctt tgtcagtgaa
      481 gatgatagtg tgatctatta tagactaggg ttcattttta atgaattcta tctcaatatt
      541 tcaggctgga gacaagaact aaagactaag cttactagta tt
//
LOCUS       DAUCWU010000182          575 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.186, whole genome shotgun sequence.
ACCESSION   DAUCWU010000182 DAUCWU010000000
VERSION     DAUCWU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.186"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>575
                     /locus_tag="VGY24_001791"
     CDS             <1..>575
                     /locus_tag="VGY24_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ4284735.1"
                     /translation="IKNHDIVFKNVSFSYDDRPILKNVSAEIKENTMTAIVGPSGSGK
                     TTFCNLIARFWDVNSGEIFIGGKNIKDYKIENLMNSISMVFQDVYLFEDTIENNIKFG
                     KQNASHEEVVQAAKKARCHEFIEALPEGYDTIIGEGGASLSGGEKQRISIARAMLKDA
                     DIIIFDEATANIDPENEDKLKEAIETLTKNK"
BASE COUNT          239 a           72 c          104 g          160 t
ORIGIN      
        1 tataaaaaat catgatatag tctttaaaaa tgtttctttt tcttatgatg atagaccaat
       61 tttaaaaaat gtatcagcag agataaaaga aaataccatg actgctattg taggaccatc
      121 tggatctggc aagactacat tttgtaatct tattgcaaga ttttgggatg taaactctgg
      181 agaaattttc ataggtggaa agaatattaa ggactataag atagaaaact taatgaattc
      241 tatttccatg gtctttcagg atgtatacct atttgaagat acaattgaaa acaatataaa
      301 atttggaaaa caaaatgcaa gccatgaaga agtagttcaa gctgcaaaaa aagcaagatg
      361 tcatgaattt atagaagctt taccagaagg atatgacact attattgggg aaggtggagc
      421 aagcctatct ggtggagaaa aacaaagaat ttccatagct agagccatgc taaaagatgc
      481 agatataatt atctttgatg aagcaactgc aaatattgac ccagaaaatg aagataaact
      541 taaagaagca atagaaacat taacaaaaaa taaga
//
LOCUS       DAUCWU010000183          536 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.187, whole genome shotgun sequence.
ACCESSION   DAUCWU010000183 DAUCWU010000000
VERSION     DAUCWU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.187"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(19..92)
                     /locus_tag="VGY24_001792"
     tRNA            complement(19..92)
                     /locus_tag="VGY24_001792"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Ile,seq:cat)
     gene            complement(113..186)
                     /locus_tag="VGY24_001793"
     tRNA            complement(113..186)
                     /locus_tag="VGY24_001793"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Met,seq:cat)
     gene            complement(192..265)
                     /locus_tag="VGY24_001794"
     tRNA            complement(192..265)
                     /locus_tag="VGY24_001794"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Pro,seq:tgg)
     gene            complement(272..345)
                     /locus_tag="VGY24_001795"
     tRNA            complement(272..345)
                     /locus_tag="VGY24_001795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Arg,seq:acg)
     gene            complement(362..446)
                     /locus_tag="VGY24_001796"
     tRNA            complement(362..446)
                     /locus_tag="VGY24_001796"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Leu,seq:taa)
     gene            complement(455..526)
                     /locus_tag="VGY24_001797"
     tRNA            complement(455..526)
                     /locus_tag="VGY24_001797"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Gly,seq:gcc)
BASE COUNT          145 a          155 c          122 g          114 t
ORIGIN      
        1 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
       61 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      121 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      181 accgccatta atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta
      241 ctctaccaag ctgagctact tcccgaactg atgcacccta gaggagtcga acctctaacc
      301 gcctgattcg tagtcaggta ctctatccag ttgagctaag ggtgctattt caaaaaatta
      361 atgccgagga ccggaatcga accggtacga tgtaaaccat cgcaggattt taagtcctgt
      421 gcgtctgcca gttccgccac cccggctcct caaaagcgaa cgacgggatt cgaacccgcg
      481 acccccacct tggcaaggtg atgttctacc actgaactac gttcgcaact aatctc
//
LOCUS       DAUCWU010000184          521 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.188, whole genome shotgun sequence.
ACCESSION   DAUCWU010000184 DAUCWU010000000
VERSION     DAUCWU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.188"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>521)
                     /locus_tag="VGY24_001798"
     CDS             complement(<1..>521)
                     /locus_tag="VGY24_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ4284736.1"
                     /translation="LLRNIYSTIVYEYSDTVIDFKTSHNLVTKKLDVRDARDFFINSE
                     MDEYAANDFKAGDKIAVFSVPFDWNYLSEGKVTAYTYGGITPYQKTSIPKNIPVNLWI
                     NGKQISVPYNEISTNKTTVTAQEIDLKVRKFLIAQHQLYSSGSSYKSGKLVFHTNDNS
                     DKYSFDLFYTGYR"
BASE COUNT          163 a           79 c           85 g          194 t
ORIGIN      
        1 tctatatcct gtatagaaaa gatcgaaaga atatttatct gaattatcat ttgtatgaaa
       61 aactaattta ccacttttgt agctagaacc agaagaatat aattgatgtt gtgctattaa
      121 aaattttcta acctttagat caatttcttg agctgtaact gttgttttgt tagttgatat
      181 ttcgttgtaa ggaacagaga tctgctttcc attaatccat aaattaacag ggatattttt
      241 aggtattgaa gttttttggt agggtgttat tccgccgtac gtatatgctg tgacttttcc
      301 ttctgacaag tagttccaat caaatgggac ggagaacaca gctattttat ctccagcttt
      361 aaaatcattg gctgcatatt cgtccatttc ggagttaata aaaaaatctc tagcatctct
      421 aacatcaagt ttcttagtca ctaagttatg actggtttta aaatcaatta ctgtatctga
      481 atattcatat acaatcgtac tatagatatt ccttagaagg g
//
LOCUS       DAUCWU010000185          511 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.189, whole genome shotgun sequence.
ACCESSION   DAUCWU010000185 DAUCWU010000000
VERSION     DAUCWU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.189"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            170..442
                     /locus_tag="VGY24_001799"
     CDS             170..442
                     /locus_tag="VGY24_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEQ4284737.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            429..>511
                     /locus_tag="VGY24_001800"
     CDS             429..>511
                     /locus_tag="VGY24_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ4284738.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEES"
BASE COUNT          226 a           53 c           87 g          145 t
ORIGIN      
        1 ttaaataata atttgaaaca acttttagac tgaatagtat taatgacaat ggaacgaact
       61 agctctttat acttttgtca aaaatctaag atctttgctg tgaaattagg atcttttcta
      121 ccatttaaaa aataaaaatg ataaaatgaa gaggattgta ggagaaatta tgagactaga
      181 taaatatcta aaggtatcgc gccttattaa acgtcgttca gtagcaaaag aagttgcgga
      241 taagggacga attaaagtta atgggatact tgctaaaagt tcaacgaata taaaactaaa
      301 tgatcacatt gaaattagtt ttggaaataa attactgaca gtaagagtca ttgaaataaa
      361 agatagtaca aaaaaagaag atgctcttaa gatgtatgag ataatcagtg aaacaaggat
      421 aacattaaat gaagaagcct agtattgttc aattaaataa ccattatatt aagaaagaga
      481 atctcaaaaa aaaatttgaa gaagaagaat c
//
LOCUS       DAUCWU010000186          482 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.190, whole genome shotgun sequence.
ACCESSION   DAUCWU010000186 DAUCWU010000000
VERSION     DAUCWU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.190"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>482)
                     /locus_tag="VGY24_001801"
     CDS             complement(<1..>482)
                     /locus_tag="VGY24_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEQ4284739.1"
                     /translation="GYDTLRVLLCDKAILVEGPSDELIIQRAYKDKYGKLPIFNGIDI
                     ISVGTSFLRFLELGDKLDLNLYVVTDNDGDVEALMRKYKDYEYNEKIKIYYDEKTYSG
                     DMDKFNYNTLEPCILRANDLKTLNSIFNTNKTDSDSILKYMEKNKTNCAVSIFESESK
                     "
BASE COUNT          152 a           77 c           49 g          204 t
ORIGIN      
        1 tttttgattc tgattcgaat atacttacgg cacaatttgt tttatttttc tccatgtatt
       61 ttaatataga gtcactatct gttttattag tattaaatat actattgaga gttttcaagt
      121 catttgctct taaaatacag ggctctaaag tattgtaatt aaatttatcc atatctccac
      181 tataagtctt ttcgtcataa tatattttaa tcttttcatt atattcataa tctttgtatt
      241 ttctcattaa agcttctaca tctccgtcat tatctgtaac tacatagaga tttaagtcta
      301 atttatctcc taattccaaa aatcttaaaa aagatgttcc aacagaaatt atatcgatac
      361 cattaaaaat tggtaatttg ccatatttat ctttatatgc tctttgtata attagttcgt
      421 ctgaaggacc ttcaactagt atagctttat cacataataa tactcgtaat gtgtcatatc
      481 ca
//
LOCUS       DAUCWU010000187          466 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.191, whole genome shotgun sequence.
ACCESSION   DAUCWU010000187 DAUCWU010000000
VERSION     DAUCWU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.191"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..87
                     /locus_tag="VGY24_001802"
     CDS             <1..87
                     /locus_tag="VGY24_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ4284740.1"
                     /translation="DSFFKRYKDNKIFNSEEISHFDIYLKTY"
BASE COUNT          156 a           64 c           83 g          163 t
ORIGIN      
        1 gacagtttct ttaaaaggta taaagataat aagattttta atagtgaaga aattagtcat
       61 tttgatatct acttaaaaac gtactagtgc tattctcccc ctttgctttt taagtattca
      121 aatgattgcc atttttagat aatgagagta agtaatgtta agaagatagc atgagaaagt
      181 atttacaggt gattggggct tcaattgcct ttgtaggtgt ggccttatgc attgatcata
      241 tgcatgatgg ggttattacc cgtgttgaaa tgataaaatc cttaaaataa gttattacgg
      301 taaagcggcc tcagggcctt ttttcatgat atttttagga aatatgaaat cctattttct
      361 cttaaaaaac cactatgatg agaatatgct tgcattgaca cattttttat gtctgaaaaa
      421 acgaaaagct tttcaaaaaa cctagctttt tcacaggtga aaacca
//
LOCUS       DAUCWU010000188          421 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.192, whole genome shotgun sequence.
ACCESSION   DAUCWU010000188 DAUCWU010000000
VERSION     DAUCWU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.192"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(52..133)
                     /locus_tag="VGY24_001803"
     tRNA            complement(52..133)
                     /locus_tag="VGY24_001803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Leu,seq:tag)
     gene            complement(139..211)
                     /locus_tag="VGY24_001804"
     tRNA            complement(139..211)
                     /locus_tag="VGY24_001804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Lys,seq:ttt)
     gene            complement(243..315)
                     /locus_tag="VGY24_001805"
     tRNA            complement(243..315)
                     /locus_tag="VGY24_001805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Asp,seq:gtc)
     gene            complement(333..405)
                     /locus_tag="VGY24_001806"
     tRNA            complement(333..405)
                     /locus_tag="VGY24_001806"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Val,seq:tac)
BASE COUNT          108 a          117 c           91 g          105 t
ORIGIN      
        1 tgattacaaa tcagatgctc taccaactga gctaagccgg ctaatctttt atgcgggtta
       61 agggacttga acccccacgc cgttaagcgc cagatcctaa atctggtgcg tctgccaatt
      121 ccgccaaacc cgcttttatg acccgtactg ggctcgaacc agtgacccat tgattaaaag
      181 tcaattgctc taccaactga gctaacgagt ctttccttca aaaaatatga ctactctttg
      241 aacggtcccg acgggaatcg aacccgcgat cttcgccgtg acagggcgac gtgataaccg
      301 ctacactacg ggacctgtat tttccaatac tatgggagtt aacgggatcg aaccgctgac
      361 cctctgcttg taaggcagat gctctcccag ctgagctaaa ctcccattga gtgtgtcatt
      421 a
//
LOCUS       DAUCWU010000189          363 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.194, whole genome shotgun sequence.
ACCESSION   DAUCWU010000189 DAUCWU010000000
VERSION     DAUCWU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.194"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(66..>363)
                     /gene="mscL"
                     /locus_tag="VGY24_001807"
     CDS             complement(66..>363)
                     /gene="mscL"
                     /locus_tag="VGY24_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ4284741.1"
                     /translation="IGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLSWNGVKY
                     GSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK"
BASE COUNT          121 a           68 c           62 g          112 t
ORIGIN      
        1 agggagcttt ttttgatatc aaaaaaagca gttagacatg cctaactact ttatcaaaaa
       61 tcgctttatt tttgagccaa caaatcacgg atttctgtca ataattcttc ttgagtagga
      121 gctgcagctt ctttttcttt tttaggcata gctttttcag cggcttttac aacaaagaaa
      181 agactggtac cgatgataag aaaattaata acggctccta aaaagctacc atatttaaca
      241 ccattccatg acaattgagt gatgttttca acatttgcag ctttcaaagc tgggtttaaa
      301 ataagcggtg tgataatatc gttcacaaaa gaagtgacaa tagcaccaaa agctcccccg
      361 atg
//
LOCUS       DAUCWU010000190          351 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.195, whole genome shotgun sequence.
ACCESSION   DAUCWU010000190 DAUCWU010000000
VERSION     DAUCWU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.195"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>351
                     /locus_tag="VGY24_001808"
     CDS             <1..>351
                     /locus_tag="VGY24_001808"
                     /inference="COORDINATES: protein motif:HMM:NF014430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEQ4284742.1"
                     /translation="KLETNIREGINLIKQINQILNRILSQMIEISEPLEEYKEAKKFS
                     GISEKLAVQIVAEFGDLSKFKNKKSLISFIGIDAPPYESGNFKADQRKISKRGNAILR
                     KVGYQAMKCMMSAK"
BASE COUNT          175 a           50 c           55 g           71 t
ORIGIN      
        1 caaaactaga aacgaatatc agagaaggaa taaacctgat aaaacaaata aatcagattc
       61 taaatagaat tttatcacaa atgatagaaa tatccgagcc cttagaagaa tataaagaag
      121 caaaaaaatt ctcaggcata tcagaaaaat tagcagtaca aatagtagca gaatttggag
      181 acctatcaaa atttaaaaac aaaaaaagcc taatatcctt tataggaata gacgcaccac
      241 catatgaatc aggaaacttt aaagcagatc aaagaaaaat aagtaaaaga ggaaacgcaa
      301 tactaagaaa agtaggctac caagcaatga aatgtatgat gagtgctaaa a
//
LOCUS       DAUCWU010000191          334 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.198, whole genome shotgun sequence.
ACCESSION   DAUCWU010000191 DAUCWU010000000
VERSION     DAUCWU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.198"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(46..>334)
                     /locus_tag="VGY24_001809"
     CDS             complement(46..>334)
                     /locus_tag="VGY24_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ4284743.1"
                     /translation="FTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFE
                     NEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS"
BASE COUNT          104 a           66 c           49 g          115 t
ORIGIN      
        1 ctgctagatc tcacctgtta aacaaaaaaa ggacaagtgt cctttttaag atttaatatt
       61 ggcagtggca acatcatctc caaaccattt ctcagaaata gcttgaaatt tgccattttg
      121 ataaagcacc ctgaaagcac gattaatttt tgcttgcaac gttttgtctt cttttctaag
      181 tccaaccgaa aatgcttcat tttcaaaggt cgttgggatc atccgataat tctccaattg
      241 cccttctttt gctaaatagt aattggcata tactttgtca atcaatattc catcgatacg
      301 atctgatttt aaatcaataa aagcttgtgt aaag
//
LOCUS       DAUCWU010000192          332 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.199, whole genome shotgun sequence.
ACCESSION   DAUCWU010000192 DAUCWU010000000
VERSION     DAUCWU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.199"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>332)
                     /locus_tag="VGY24_001810"
     CDS             complement(<1..>332)
                     /locus_tag="VGY24_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEQ4284744.1"
                     /translation="SQYLFNREVVDEYLTNINSNSPCALPEIIIEIYFSELDDANLEG
                     SGNGLGLKEEIGFAFKVLFDNSYEDEYKLFMEKGELSSLPIEYYKSEWLGFNRENITT
                     RSIPSKSF"
BASE COUNT          110 a           56 c           34 g          132 t
ORIGIN      
        1 aaaaatgatt ttgaaggaat gcttctagta gtaatatttt ctctattaaa gcctagccat
       61 tcacttttat aatattctat aggtaaactg cttaattctc ctttttccat aaatagttta
      121 tattcatctt catacgaatt atcaaataat actttaaatg caaatcctat ttcttctttt
      181 aatcctaagc cgttaccact accctccaaa tttgcatcat ctaattcaga gaagtatatt
      241 tctattataa tttctggtaa tgcacaagga ctgttggaat ttatattggt taaatattca
      301 tctacaactt ctctattgaa taaatattga ga
//
LOCUS       DAUCWU010000193          304 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.201, whole genome shotgun sequence.
ACCESSION   DAUCWU010000193 DAUCWU010000000
VERSION     DAUCWU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.201"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            5..193
                     /locus_tag="VGY24_001811"
     CDS             5..193
                     /locus_tag="VGY24_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ4284745.1"
                     /translation="MLTYDEFKQAIDNGYITGDTVIIVRKNGQIFDYVLPGEKVRPWE
                     VVTEEVVEEVMVELDYIK"
BASE COUNT          119 a           37 c           72 g           76 t
ORIGIN      
        1 ctttatgcta acatacgacg aatttaagca agcaattgac aacggatata tcacaggaga
       61 cacagtaata atcgtgcgca aaaacggaca gatttttgat tatgtgttgc ctggtgagaa
      121 agtcagacca tgggaggttg tgaccgagga ggtagtggaa gaagtgatgg tggaattaga
      181 ctatatcaaa tgaagaaaac tttgagtaga cagaaaaata attgaagagt tgtcaattta
      241 gttgtcaata atatagaaag ttatggtaac tcatgaacaa gacaaaaaga aaaaaccttg
      301 atgt
//
LOCUS       DAUCWU010000194          289 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.202, whole genome shotgun sequence.
ACCESSION   DAUCWU010000194 DAUCWU010000000
VERSION     DAUCWU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.202"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>289
                     /locus_tag="VGY24_001812"
     CDS             <1..>289
                     /locus_tag="VGY24_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEQ4284746.1"
                     /translation="KLYIAAAGAGKTTLIVKDALKNKDKKILITTYTNANCDSIKKKI
                     IKINGAIPKNIEVLTWFSFLLKHGLRPYQDIMFSQRINGIVPVNGKSAIFKN"
BASE COUNT          126 a           29 c           45 g           89 t
ORIGIN      
        1 aaattatata tagctgctgc tggagcaggt aagacaacat taattgtgaa ggatgcttta
       61 aaaaataaag ataaaaagat tttgataaca acatatacaa atgcaaattg tgattctata
      121 aaaaagaaaa taattaaaat aaatggagcc attcctaaaa atattgaagt tttaacatgg
      181 ttttcatttt tacttaaaca tggattaaga ccatatcaag atataatgtt tagtcagaga
      241 attaatggaa tagtacctgt caacggtaag agtgctatat ttaaaaata
//
LOCUS       DAUCWU010000195          262 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.denovo.208, whole genome shotgun sequence.
ACCESSION   DAUCWU010000195 DAUCWU010000000
VERSION     DAUCWU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.denovo.208"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          106 a           24 c           54 g           78 t
ORIGIN      
        1 gataagaggg acattagata acgaagattt aatttccata tcaaaagctt ttagagaaac
       61 taaggatgat tttagtaaga atgaaagtgt taagaatatt aacgaaaaat taagtggatc
      121 caaatcaaat ttgggaaaaa aagttagttt aggtgttaat ttagggagta atttcaactg
      181 ggaatcaagt atgataacgg aatttgatag tattccatat tccaatgctg gtagaggact
      241 acaatgctta ttaaaaactg ag
//
LOCUS       DAUCWU010000196         2151 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.guided.138, whole genome shotgun sequence.
ACCESSION   DAUCWU010000196 DAUCWU010000000
VERSION     DAUCWU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.guided.138"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            46..162
                     /locus_tag="VGY24_001813"
     CDS             46..162
                     /locus_tag="VGY24_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEQ4284747.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            178..2097
                     /gene="tet(M)"
                     /locus_tag="VGY24_001814"
     CDS             178..2097
                     /gene="tet(M)"
                     /locus_tag="VGY24_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ4284748.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLKALELEQEESIRFHNCSLFSVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          737 a          330 c          431 g          653 t
ORIGIN      
        1 ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat
       61 ccagagaata tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa
      121 agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg
      181 aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa
      241 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
      301 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      361 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      421 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      481 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      541 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      601 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      661 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      721 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat taaaagcatt ggaactcgaa
      781 caagaggaaa gcataagatt tcataattgt tccctgttct ctgtttatca cggaagtgca
      841 aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt ttattcatca
      901 acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa
      961 agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
     1021 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
     1081 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
     1141 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
     1201 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
     1261 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
     1321 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     1381 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
     1441 gaaagaccgt taaaaaaagc agagtatacc attcacatcg aagttccacc gaatcctttc
     1501 tgggcttcca ttggtctatc tgtagcaccg cttccattag ggagcggagt acagtatgag
     1561 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata
     1621 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
     1681 aagtatggct tatactatag ccctgttagt accccagcag attttcgaat gcttgctcct
     1741 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1801 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1861 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1921 cctgctcgat gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1981 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     2041 cgtcgtccaa atagtaggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     2101 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat t
//
LOCUS       DAUCWU010000197         1856 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.guided.147, whole genome shotgun sequence.
ACCESSION   DAUCWU010000197 DAUCWU010000000
VERSION     DAUCWU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.guided.147"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..356
                     /locus_tag="VGY24_001815"
     CDS             <1..356
                     /locus_tag="VGY24_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEQ4284749.1"
                     /translation="HELLRMISWKVGIETGFKLSVGKNYKFIERYISEDLWEKLLSTY
                     RMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMYKKYTGKTGC
                     LDSTYAADIEERREQ"
     gene            353..895
                     /gene="sat4"
                     /locus_tag="VGY24_001816"
     CDS             353..895
                     /gene="sat4"
                     /locus_tag="VGY24_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEQ4284750.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            988..1782
                     /locus_tag="VGY24_001817"
                     /note="aph(3')-IIIa"
     CDS             988..1782
                     /locus_tag="VGY24_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEQ4284751.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          616 a          309 c          453 g          478 t
ORIGIN      
        1 gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa
       61 gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac
      121 ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc
      181 atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg
      241 agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa
      301 ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac
      361 agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat
      421 aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga
      481 agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg
      541 ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag acgataaatg
      601 cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc
      661 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
      721 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
      781 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
      841 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
      901 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
      961 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     1021 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     1081 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     1141 gggaccacct atgatgtgga acgagaaaag gacatgatgc tatggctgga aggaaagctg
     1201 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     1261 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     1321 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     1381 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     1441 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     1501 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     1561 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     1621 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccgatcgat cagggaggat
     1681 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     1741 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
     1801 taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta catccg
//
LOCUS       DAUCWU010000198         1279 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.guided.163, whole genome shotgun sequence.
ACCESSION   DAUCWU010000198 DAUCWU010000000
VERSION     DAUCWU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.guided.163"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..189
                     /locus_tag="VGY24_001818"
     CDS             <1..189
                     /locus_tag="VGY24_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ4284752.1"
                     /translation="LEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGM
                     ADEMRRPMMLIVSAKKKM"
     gene            222..1130
                     /locus_tag="VGY24_001819"
     CDS             222..1130
                     /locus_tag="VGY24_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEQ4284753.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1127..>1279
                     /locus_tag="VGY24_001820"
     CDS             1127..>1279
                     /locus_tag="VGY24_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ4284754.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQ"
BASE COUNT          459 a          184 c          283 g          353 t
ORIGIN      
        1 ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa tacactgctt
       61 tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa catgatggat
      121 attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag
      181 aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca gaaaaagaaa
      241 tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt gtgacccttg
      301 aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat gatattacat
      361 attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat caatttggga
      421 atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa gaaaagggat
      481 tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta ttgcccttgg
      541 aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt gataaagatt
      601 gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga aagccaagcg
      661 caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat gttattaaag
      721 gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt gttcgccatg
      781 agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag
      841 gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag aaacttttgt
      901 ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta tgccatcaat
      961 tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat ccggagtatg
     1021 ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt aaaaccggct
     1081 gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga ttacagaaat
     1141 gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa
     1201 gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc
     1261 atatttaaaa agctaccaa
//
LOCUS       DAUCWU010000199          899 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG134112
            SAMN37094604-rid19215533.guided.174, whole genome shotgun sequence.
ACCESSION   DAUCWU010000199 DAUCWU010000000
VERSION     DAUCWU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37094604
            Sequence Read Archive: SRR25711375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001859349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001859349.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/23/2023 18:29:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,822
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 16 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37094604-rid19215533.guided.174"
                     /strain="TG134112"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA"
                     /collection_date="2019-07-18"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..103
                     /locus_tag="VGY24_001821"
     CDS             <1..103
                     /locus_tag="VGY24_001821"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEQ4284755.1"
                     /translation="FISFIMKKIKGGKGMGMFSIFVIERFHYQPNQK"
     gene            161..892
                     /gene="erm(A)"
                     /locus_tag="VGY24_001822"
     CDS             161..892
                     /gene="erm(A)"
                     /locus_tag="VGY24_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEQ4284756.1"
                     /translation="MKQKNPKNTQNFITSKKHVKKILKYTNINKQDKIIEIGSGKGHF
                     TKELVEMSQRVNAIEIDEGLCHATKKAVEPFQNIKVIHEDILKFSFPKNTDYKIFGNI
                     PYNISTDIVKKIAFDSQAKYSYLIVERGFAKRLQNTQRALGLLLMVEMDIKILKKVPR
                     AYFHPKPNVDSVLIVLERHKPFILKKDYKKYRFFVYKWVNREYHVLFTKNQLRQVLKH
                     ANVTDLDKLSNEQFLSVFNSYKLFQ"
BASE COUNT          354 a          106 c          154 g          285 t
ORIGIN      
        1 ctttatctca tttataatga aaaaaattaa aggaggtaaa ggtatgggta tgttttctat
       61 atttgttatt gagagatttc attatcaacc aaaccaaaaa taattggtta taatgagctc
      121 ttaagattag ttaattataa ccggcaagga gaaggttatt atgaaacaga aaaacccgaa
      181 aaatacgcaa aatttcatta catctaaaaa gcatgtaaag aaaatattaa aatatacgaa
      241 tatcaataaa caagataaaa taatagaaat tgggtcagga aaaggacatt ttaccaagga
      301 acttgtggaa atgagtcaac gggtgaatgc tatagagatt gatgaaggtt tatgtcatgc
      361 cacgaaaaaa gcagttgaac cttttcagaa tataaaagtt attcatgagg atattttgaa
      421 gtttagcttt cctaaaaata cagactataa aatatttggt aatattccct acaatattag
      481 tactgatatt gtaaaaaaga ttgcttttga tagtcaagcg aaatatagct accttattgt
      541 agagagggga tttgctaaaa ggttgcaaaa tacccaacga gctttaggtt tgctgttaat
      601 ggtggaaatg gatataaaaa ttcttaaaaa agtgccacga gcatattttc accctaagcc
      661 taatgtagat tctgtattga ttgtacttga aaggcataaa ccatttattt taaagaagga
      721 ctacaaaaag tatagatttt tcgtttataa atgggtaaac agggaatatc atgttctttt
      781 tactaaaaat caattaagac aggtgctgaa gcatgcgaat gttactgatc ttgataaatt
      841 atccaatgaa caatttttgt ctgttttcaa tagttacaaa ttatttcaat aaattaaaa
//
